LOCUS       DAVBDG010000001        72202 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBDG010000001 DAVBDG010000000
VERSION     DAVBDG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 72202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.001"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..275
                     /locus_tag="VQ526_000001"
     CDS             <1..275
                     /locus_tag="VQ526_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2553785.1"
                     /translation="YQHDSYHRFLESKGIQASMSRKGNSQDNGMMESFFGILKSEMFY
                     GYEKTFKSLNQLEQAIIDYIDYYNNKKIKLKLKGLSPVQYRTKSFG"
     gene            complement(400..603)
                     /locus_tag="VQ526_000002"
     CDS             complement(400..603)
                     /locus_tag="VQ526_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2553786.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(596..1678)
                     /locus_tag="VQ526_000003"
     CDS             complement(596..1678)
                     /locus_tag="VQ526_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2553787.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1804..2586)
                     /locus_tag="VQ526_000004"
     CDS             complement(1804..2586)
                     /locus_tag="VQ526_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2553788.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2586..3929)
                     /locus_tag="VQ526_000005"
     CDS             complement(2586..3929)
                     /locus_tag="VQ526_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2553789.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKKCTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4028..5344)
                     /locus_tag="VQ526_000006"
     CDS             complement(4028..5344)
                     /locus_tag="VQ526_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2553790.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQNLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5537..6763
                     /locus_tag="VQ526_000007"
     CDS             5537..6763
                     /locus_tag="VQ526_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET2553791.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYILWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6962..8536
                     /locus_tag="VQ526_000008"
     CDS             6962..8536
                     /locus_tag="VQ526_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2553792.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8800..9588
                     /gene="codY"
                     /locus_tag="VQ526_000009"
     CDS             8800..9588
                     /gene="codY"
                     /locus_tag="VQ526_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2553793.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGQLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9588..10163
                     /locus_tag="VQ526_000010"
     CDS             9588..10163
                     /locus_tag="VQ526_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2553794.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10245..10330
                     /locus_tag="VQ526_000011"
     tRNA            10245..10330
                     /locus_tag="VQ526_000011"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10279..10281,aa:Leu,seq:aag)
     gene            10613..11743
                     /gene="ugpC"
                     /locus_tag="VQ526_000012"
     CDS             10613..11743
                     /gene="ugpC"
                     /locus_tag="VQ526_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2553795.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            12244..13002
                     /locus_tag="VQ526_000013"
     CDS             12244..13002
                     /locus_tag="VQ526_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2553796.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            13089..13601
                     /locus_tag="VQ526_000014"
     CDS             13089..13601
                     /locus_tag="VQ526_000014"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2553797.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13783..14727
                     /locus_tag="VQ526_000015"
     CDS             13783..14727
                     /locus_tag="VQ526_000015"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2553798.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14736..15413
                     /locus_tag="VQ526_000016"
     CDS             14736..15413
                     /locus_tag="VQ526_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2553799.1"
                     /translation="MTYLDAFKSGILVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            15511..16269
                     /locus_tag="VQ526_000017"
     CDS             15511..16269
                     /locus_tag="VQ526_000017"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2553800.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16307..17812
                     /gene="lytC"
                     /locus_tag="VQ526_000018"
     CDS             16307..17812
                     /gene="lytC"
                     /locus_tag="VQ526_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2553801.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKVIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18309..18827
                     /locus_tag="VQ526_000019"
     CDS             18309..18827
                     /locus_tag="VQ526_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2553802.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEASEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            18957..19463
                     /locus_tag="VQ526_000020"
     CDS             18957..19463
                     /locus_tag="VQ526_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2553803.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEL"
     gene            19665..20897
                     /gene="clpX"
                     /locus_tag="VQ526_000021"
     CDS             19665..20897
                     /gene="clpX"
                     /locus_tag="VQ526_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2553804.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20906..21493
                     /locus_tag="VQ526_000022"
     CDS             20906..21493
                     /locus_tag="VQ526_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2553805.1"
                     /translation="MELNIHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21504..21884
                     /locus_tag="VQ526_000023"
     CDS             21504..21884
                     /locus_tag="VQ526_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2553806.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21936..22826
                     /gene="rapZ"
                     /locus_tag="VQ526_000024"
     CDS             21936..22826
                     /gene="rapZ"
                     /locus_tag="VQ526_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2553807.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22823..23800
                     /locus_tag="VQ526_000025"
     CDS             22823..23800
                     /locus_tag="VQ526_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2553808.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVVGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQKYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23797..24708
                     /gene="whiA"
                     /locus_tag="VQ526_000026"
     CDS             23797..24708
                     /gene="whiA"
                     /locus_tag="VQ526_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2553809.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24742..25710)
                     /locus_tag="VQ526_000027"
     CDS             complement(24742..25710)
                     /locus_tag="VQ526_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2553810.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25846..26703
                     /locus_tag="VQ526_000028"
     CDS             25846..26703
                     /locus_tag="VQ526_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2553811.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26690..27469
                     /locus_tag="VQ526_000029"
     CDS             26690..27469
                     /locus_tag="VQ526_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2553812.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27493..28845
                     /locus_tag="VQ526_000030"
     CDS             27493..28845
                     /locus_tag="VQ526_000030"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2553813.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28919..29293
                     /locus_tag="VQ526_000031"
     CDS             28919..29293
                     /locus_tag="VQ526_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2553814.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPNELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29295..30143
                     /locus_tag="VQ526_000032"
     CDS             29295..30143
                     /locus_tag="VQ526_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2553815.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30420..31187
                     /locus_tag="VQ526_000033"
     CDS             30420..31187
                     /locus_tag="VQ526_000033"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2553816.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLACFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRTQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEIRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31199..32383
                     /locus_tag="VQ526_000034"
     CDS             31199..32383
                     /locus_tag="VQ526_000034"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2553817.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32380..34251
                     /locus_tag="VQ526_000035"
     CDS             32380..34251
                     /locus_tag="VQ526_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2553818.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTILSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELVWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34394..35578)
                     /gene="mntE"
                     /locus_tag="VQ526_000036"
     CDS             complement(34394..35578)
                     /gene="mntE"
                     /locus_tag="VQ526_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2553819.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLATGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35882..38578
                     /locus_tag="VQ526_000037"
     CDS             35882..38578
                     /locus_tag="VQ526_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2553820.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLVEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILAIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38591..39382
                     /gene="yghU"
                     /locus_tag="VQ526_000038"
     CDS             38591..39382
                     /gene="yghU"
                     /locus_tag="VQ526_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2553821.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39498..39908
                     /locus_tag="VQ526_000039"
     CDS             39498..39908
                     /locus_tag="VQ526_000039"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2553822.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39920..41515
                     /locus_tag="VQ526_000040"
     CDS             39920..41515
                     /locus_tag="VQ526_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2553823.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            41582..41746
                     /locus_tag="VQ526_000041"
     CDS             41582..41746
                     /locus_tag="VQ526_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2553824.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            41825..42553
                     /gene="yaaA"
                     /locus_tag="VQ526_000042"
     CDS             41825..42553
                     /gene="yaaA"
                     /locus_tag="VQ526_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2553825.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPVLSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDETLKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTLKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(42830..43378)
                     /locus_tag="VQ526_000043"
     CDS             complement(42830..43378)
                     /locus_tag="VQ526_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2553826.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            43699..44187
                     /locus_tag="VQ526_000044"
     CDS             43699..44187
                     /locus_tag="VQ526_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2553827.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            44184..45371
                     /locus_tag="VQ526_000045"
     CDS             44184..45371
                     /locus_tag="VQ526_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2553828.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            45364..45729
                     /locus_tag="VQ526_000046"
     CDS             45364..45729
                     /locus_tag="VQ526_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2553829.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKMAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            45745..47091
                     /gene="asnS"
                     /locus_tag="VQ526_000047"
     CDS             45745..47091
                     /gene="asnS"
                     /locus_tag="VQ526_000047"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2553830.1"
                     /translation="MMTKRVTIIDVKDYIGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            47244..47534
                     /locus_tag="VQ526_000048"
     CDS             47244..47534
                     /locus_tag="VQ526_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2553831.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            47546..48016
                     /gene="ssbA"
                     /locus_tag="VQ526_000049"
     CDS             47546..48016
                     /gene="ssbA"
                     /locus_tag="VQ526_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2553832.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            48048..48287
                     /locus_tag="VQ526_000050"
     CDS             48048..48287
                     /locus_tag="VQ526_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2553833.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            48582..49982
                     /locus_tag="VQ526_000051"
     CDS             48582..49982
                     /locus_tag="VQ526_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2553834.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGLLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            49984..50343
                     /locus_tag="VQ526_000052"
     CDS             49984..50343
                     /locus_tag="VQ526_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2553835.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            50397..51146
                     /locus_tag="VQ526_000053"
     CDS             50397..51146
                     /locus_tag="VQ526_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2553836.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            51136..51435
                     /locus_tag="VQ526_000054"
     CDS             51136..51435
                     /locus_tag="VQ526_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2553837.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            51506..52441
                     /locus_tag="VQ526_000055"
     CDS             51506..52441
                     /locus_tag="VQ526_000055"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2553838.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            52527..53039
                     /locus_tag="VQ526_000056"
     CDS             52527..53039
                     /locus_tag="VQ526_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2553839.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            53076..53671
                     /locus_tag="VQ526_000057"
                     /pseudo
     CDS             53076..53671
                     /locus_tag="VQ526_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            53772..53966
                     /locus_tag="VQ526_000058"
     CDS             53772..53966
                     /locus_tag="VQ526_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2553840.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(54012..55457)
                     /locus_tag="VQ526_000059"
     CDS             complement(54012..55457)
                     /locus_tag="VQ526_000059"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2553841.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HSSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            55563..57185
                     /locus_tag="VQ526_000060"
     CDS             55563..57185
                     /locus_tag="VQ526_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2553842.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            57388..59346
                     /locus_tag="VQ526_000061"
     CDS             57388..59346
                     /locus_tag="VQ526_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2553843.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVVSKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGIESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            59518..60078
                     /locus_tag="VQ526_000062"
     CDS             59518..60078
                     /locus_tag="VQ526_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2553844.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTG"
     gene            60079..60453
                     /locus_tag="VQ526_000063"
     CDS             60079..60453
                     /locus_tag="VQ526_000063"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2553845.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALENLLAVPKEKFE"
     gene            60478..61200
                     /locus_tag="VQ526_000064"
     CDS             60478..61200
                     /locus_tag="VQ526_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2553846.1"
                     /translation="MNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPSGSGKSTIV
                     RLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRL
                     VTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGQSQRLQIARAYLKDAEIL
                     IFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLEEGKITGSG
                     THKELLENHERYARFVQEQMIE"
     gene            61358..62452
                     /locus_tag="VQ526_000065"
     CDS             61358..62452
                     /locus_tag="VQ526_000065"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2553847.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            62460..63626
                     /locus_tag="VQ526_000066"
     CDS             62460..63626
                     /locus_tag="VQ526_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2553848.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMSKSLLQEVCQRIVATFAKR"
     gene            63719..66817
                     /locus_tag="VQ526_000067"
     CDS             63719..66817
                     /locus_tag="VQ526_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2553849.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELSDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLSGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            66865..67482
                     /locus_tag="VQ526_000068"
     CDS             66865..67482
                     /locus_tag="VQ526_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2553850.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            67493..68827
                     /locus_tag="VQ526_000069"
     CDS             67493..68827
                     /locus_tag="VQ526_000069"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2553851.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLIRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            68837..69301
                     /locus_tag="VQ526_000070"
     CDS             68837..69301
                     /locus_tag="VQ526_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2553852.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            69298..69792
                     /locus_tag="VQ526_000071"
     CDS             69298..69792
                     /locus_tag="VQ526_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2553853.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            69873..71528
                     /gene="mltG"
                     /locus_tag="VQ526_000072"
     CDS             69873..71528
                     /gene="mltG"
                     /locus_tag="VQ526_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2553854.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTINELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTDIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            71599..72081
                     /gene="greA"
                     /locus_tag="VQ526_000073"
     CDS             71599..72081
                     /gene="greA"
                     /locus_tag="VQ526_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2553855.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT        21380 a        13472 c        16382 g        20968 t
ORIGIN      
        1 aataccaaca cgattcttat catcggttcc tagagagtaa gggaattcaa gcatctatgt
       61 cacgcaaggg aaacagccaa gacaacggta tgatggagtc cttctttggc attttaaaat
      121 ccgaaatgtt ttatggctat gagaaaacat ttaaatcact taatcaattg gaacaagcca
      181 ttatagacta tattgattat tacaacaata agaaaattaa gttaaaacta aaaggactta
      241 gccctgtgca atacagaact aaatccttcg gataaattaa ttgtctaact ttttgggggc
      301 agtacacaat acctgatagt atgcgtttta ttgtgggaag attttcctca ttttctagtg
      361 aaattgaact ttttctaaca tcttttgata gatgacttct taatcaatta tcatttccat
      421 tgttgtttct tggggtttca tcccttgtct ttgataaaag cggagagcac cctcgttatc
      481 attccagaca cttagggtta gattattgca gccaatttct ttagcataat agcagaaaaa
      541 tgacaataaa atagccccta gatattttga aaggaaagca taggggctat atagtttaat
      601 caaaataaag caaatctttg ctggtgcttg taaagaggtc gaagccatcc ttggtaacaa
      661 caccgcagtc ttcaatacga acaccgactt taccagggat atagatacct ggttcaacag
      721 agaagcacat gccttcttcg atgaccatgt cgtttccttc catgatagat gggaattcat
      781 ggacatccat accgatacca tgcccgagac ggtgattgaa gtactcacca taaccagctt
      841 tttcgatgac ctcacgggca gcgcggtcca cttcatgagc agtcacacct ggcttgataa
      901 agtcaagagc agcttgttgg gcttcaagag tcaagttgta aatatctttc ttgaattggt
      961 ctggtttgcc gacagcgact gtacgagtca tatctgacgc atagccattg accagaacac
     1021 ccaggtcaaa gaggagaaga gcatcatttt caaccttatt agctgctgga atgccgtgtg
     1081 gattcgcagc attatcacca gtcaagacca tggtatcaaa gctcatttca taaccttcac
     1141 gtttcatggc aaagtcgatt tgtgcgatga tatctgtctc agtcttatca agagaaatat
     1201 tgtcaaaacc aacatgaaca gccttgtcag cataaagacc tgcaaccatc attttttgca
     1261 cttcatcagc tgatttgatg aggcgcatgc gttggatacg aggagtgagg ttgtcaaact
     1321 cagcagtctc aaaaactgtt ttcaaaccat ggtatttggt caagatgaga ttgtcaaact
     1381 caacagcgac acgtttgaag tcaagttgtg gaagagcatg tttgattttt tgccatggat
     1441 tttcagaatc gacatagccc actactggga aggaaacggt gctacttgca cgttctactt
     1501 caagagctgg gacaaagagg agaggttcct gatccgctag gacaaagagg aacatttggc
     1561 gttcatgggg atcactgtaa aaaccagtga ggtaattgat tgtgacgggg tcagatacga
     1621 cagcgacgtc tagtttttct gattcaagat atgttaggat ttgttgtaat ttagacatat
     1681 gctaccttct ttctacccct ctattttggc aaaaaatgag agaaaatgca agggagaagg
     1741 gtaaaagaaa agacaaaaaa ctccgacaag ataacggagt tctttgatat cattttcctt
     1801 tgtttaagaa aagtcagcct tgcttttgac gtcactgtac tcttcagcga tttcttggct
     1861 gaggatatcc tcataggcag ggagttggat atcctgacca tatttcttct tgagggcagt
     1921 agtaactagg cgataagcca gattggcatt acgagagagg ataggcccgt ggaagtagga
     1981 accaaagaca ttcttataat gaaccccttc accgaccttt tcttcgttgt ttccatttcc
     2041 atagacaacc tgtcccagcg gtttttggtc atcagagagg aaggtacggc cttggtgatt
     2101 ttcaaatcca tagtaggttt catcgaaatc ttcattgtga atcttgatgt caccgataaa
     2161 acggttattg gtctggttga gcgtgtagtg tcccatgacc cctagccctt cgatacgttt
     2221 tcctgaagct tcaacataat attgacccaa tagttggaaa ccaccgcaga tagccagaac
     2281 tacaccgtcg ttttggatgt agttgtcaat gctctctttt ttagcaggta ggtcgtctgc
     2341 aatgatactt tgttcaaagt cttgaccacc accgaaaaag gcgatgtcgt agtgattttc
     2401 atcaaagtca tcatggagag aaacgatgtc aacggtcaca tgggctccca gtttttcagc
     2461 cacatatttg agcatgagga tgtttccatt gtccccgtag gtattcatga gatttccgta
     2521 gaggtgggca atgttgagct gataggggta attgccatct tttgaggaaa gtgaagtata
     2581 aaccattagt tcatctcctt tctaacaatc tgacgactag ccagcagttc acgaaattcc
     2641 agcatggcag tataagttgc cagaatatag gcatgcttgc agtcttgatt ctcaatggtc
     2701 ttgagaactt gctccagatt actcgtttca gtgattttct cagctggata gccagtcact
     2761 cggaggcgac gagcgatttc agaatgacga acaccgccag cgttgatttc aggaatgtcc
     2821 atgtcagtga tttgctcaaa gtctgcatcc cagatccagc tagtgtcaat tccatctgca
     2881 tagttggcat taaggaggac agataggcta aatggataag gtgctagttt gatcatttcg
     2941 atagcttggg ttgcaccgac tggattttta atcaagacaa gggtacattt cttgtcaccg
     3001 atatgaaagg tttcttggcg tccaaagaca gcacggctct tgtcaaatcc ctgtttgatg
     3061 agttgcgaat cggcacctag gaaacgggcg atggccacag cagctagggc gttatagata
     3121 ttatagagcc cgccgatttg gataccgtat tcttggccgt ctatgacaaa gcgagagcga
     3181 ttgttggtca actcaaccag ttttgtcaaa cgatagtcga gatcaggacg tttacatcca
     3241 caaccttcac agatataggc acccaagttt gcataggtat tatgctcata tttgaggatg
     3301 ccttggcagt caggacagag aatcccttcg gtattgtagt gagccagttg ggctggtcct
     3361 ttttccaagt caaaaccaaa atactctata gggtttggaa tagttggctt gtagaaaagt
     3421 ggactgtctc cgttaaggag aacagtagca gttggaactt ttcgaatggc atccaatatc
     3481 atgttatagg tagtatagat ttcaccgaaa cggtccatct ggtcacggaa gatattagta
     3541 atgacaaaaa gactaggctg gatatagtca cagatacgag atagactggc ttcgtcaatt
     3601 tcgaggacgg caatattttt cccagttttt gaagatttgg ctgttaggaa ggttgttgca
     3661 atccctgtaa tcatgttggc accgcttggg ttggttagaa cttgaccata aacctctttt
     3721 aaaatgccga cagtgagggc agttgtcagg gtttttccat ttgttccagt gacaacgaca
     3781 atctcgtagt tcttagctag gttttgtaaa atatctttat caaattgaag ggcgacttta
     3841 cctgggagcg tacttccacg tccaagacgg cttaaaacga agtgggaaga acgcccagca
     3901 agaaggccca aagtagtttt taagttcatg tttctattat atcacaaaag agggaaaaga
     3961 cagatttggt gtttcgtaga agtaaaaaca gcccaaagag ggctgtcagt taagatgaga
     4021 tttcaactta aatcgcaaac aagtcattca agttctcagc caaggttggg tgagtgaaga
     4081 tttgttttgt gaagtaagtg taaggaatct tgttgtccat agcaacagtg atgatgttga
     4141 tgatttcttg agaaccttct gagaagatgc ttgctccaag aatttctttt gtttcagtat
     4201 tgacaacagc tttgaaggca ccgcgaaggt ctccatttac gtgaccacga ggcattgctg
     4261 caacggggat ttccttaaca gcgtatggaa gtttcaaatc agctgcttgg ctttcagtca
     4321 aaccaacttg tgaaagtgca ggtgtgatga acatagtatt tggcacattg agacggtctt
     4381 caagtgtgta gctgccatct ccagcaaggt agctgtaaac aacacggaag tcatcaagtg
     4441 aaatgtaagt aaattgaagg ccaccgttga catctccaac tgcaaagaca ccaggaacgt
     4501 ttgtttgaca gtgtttgtct actttaatag caccacgttc agttagttca atatctgtat
     4561 tttcaagttg aagtggttct acatttggtt tgcgtccagt tgcgtagaga agggcgtcga
     4621 aacggtaagt ttcgtcttca gttacgacaa gcacttggtc accatcgttt ttgatttcag
     4681 tagtatggat attttgaagc aattcaatgc catcttcttc catgtattgt ttagcaagag
     4741 ctgcgatgga aggttctgca cgaggaagga aagtatccaa ggcatctagg actgtgacct
     4801 tgcttccaag tttgttgtaa aggccggcaa attcaagacc gatatttccg ccaccaagga
     4861 ttccaagttt ttcaggtaat ttgtccaagt tttggatacc tgttgagtca aagatgtttt
     4921 tgcttgtagc aagtccaggg attggcaaga cgtttgaaac agcaccagtg ttgatgacga
     4981 ttgtttcagc agtcagttct ttcttttcat caccagcttg gatttcgatg actttatttg
     5041 aaaggaagtg agcttccgca tcaaagatat ctacgcctgt accagcaaca gtcgcatagt
     5101 ttttaccgtt gaggcgacca gtgatcgtgt ttttagtagc aatcacttct tcaaaagaca
     5161 agtccttttc agcagcaact agcaaggttt tagttgggat acaaccaatg ttgatacaag
     5221 ttccaccgta catagccttg ctgcgttcaa cgagggcaac ttttttgcca gctgaagcca
     5281 atttacctgc tagtgtttta ccagctttac caaatccaat aacaattaaa tcgtatgtta
     5341 acatttatct tcctcctatt cgaatttcat tctagcacaa gaaaaaaact tttgcacaat
     5401 tctgctacgg aaaagacaga aaaagataaa aacgctttcc agacaaaaaa ctagtaattt
     5461 tttgacaagc ttgataaact aaaatcctat ctatttttat ataagataga ataaatgttc
     5521 gaaaatagga ggttttatga aatcgaatcg ttatattatt gcctttgctg gggtcatttt
     5581 acacttaatg ctgggttcga cttatgcctg gagtgtttat cgtaacccta ttattgaaaa
     5641 aacgggatgg gatcaggctt ctgttgcctt cgcctttagt ctagcaatct tttgtttggg
     5701 cttatcggct gcatttatgg ggcgtttggt agaaaaattt ggtccgaaag tcatgggaag
     5761 tctatctgct tttctatacg caggtggaaa tatcttaaca ggatttgcaa tagaccgtca
     5821 ggagctgtgg ttgttgtatc tcgcttatgg cattttaggt gggcttggtt tgggagcagg
     5881 ctatattacc cctgtgtcga cgattataaa atggtttcct gataaacgtg gtctcgcaac
     5941 aggtttagcg attatggggt ttggttttgc ttctttattg actagtccca tagcgcaaca
     6001 cctcatcgca ggggtagggc ttgtagaaac tttttatatt ttaggagcaa gttactttat
     6061 tatcatgctc ctagcttcac aattcattaa gcgtccaaat gagcaagagc ttgcaatttt
     6121 atcttcttca gggaaagaaa aaacagcctc tttgacgcaa ggaatggctg caaatcaggc
     6181 tctaaaaagc aatcggtttt atatactttg gattattttc tttatcaaca tagcttgtgg
     6241 tttaggctta atttcagcgg catcgccaat ggcacaggag atggctggct tgtctacaag
     6301 tcatgcagca gtaatggtgg gcgttttagg gattttcaat ggatttggtc gcttgctctg
     6361 ggcgagctta tctgactata tcggtcgccc tctaaccttt agtatattac tgcttgttaa
     6421 tcttttcttt tctctctcac tttggctctt tacagattcc gttttatttg tagtcgctat
     6481 gtctattttg atgacttgct atggagctgg tttttcattg atcccagctt atctcagtga
     6541 tatttttgga accaaggaat tggccgctct gcatggatat attttaacag cttgggcaat
     6601 ggctggttta gcgggaccta ttttattagc agagacttat aaaatggctc attcgtacac
     6661 acaaaccttg ttcgtttttc tcattttata cagtatcgcc ttggctttgt cttattatct
     6721 aggtcgttca atcaaaaaag aaagtcaaaa accgcttaca tgattttctg tagaaatggg
     6781 gctatctttt gctctggtct tgtgttatac tagataggtt gcaaagaaaa cagtactttt
     6841 cttttgtgga aaagaagcaa cacgatttta tatctagtta tatgaaaata cgtcataaaa
     6901 agaaaagtat aaactaagcc ctatagggtg gcttcgcacc acctttagaa agaagaataa
     6961 cgtgaaattt aatgaattaa acttgtctgc tgatttgctg gcagaaattg aaaaagctgg
     7021 ttttgtagaa gctagtccta tccaagaaca aactattcct ttggcccttg aaggcaagga
     7081 tgttatcggt caagctcaga ctggtacagg aaaaactgca gcctttggct tgcctaccct
     7141 tgaaaaaatc cgtacagaag aagcgactat ccaagccttg gtcatcgctc caactcgtga
     7201 actagctgtc caaagtcaag aagaactctt ccgctttggt cgtagtaagg gagtcaaagt
     7261 ccgttcagta tatggcggat caagcattga aaaacaaatt aaggctctta aatctggtgc
     7321 ccatatcgtg gtgggaactc caggtcgcct cttggacttg attaaacgca aggccttgaa
     7381 attacaaaac attgaaaccc ttatccttga cgaagcggat gaaatgctta acatgggctt
     7441 ccttgaagac atcgaagcca ttatttcccg tgtacctgag aaccgtcaaa ctttgctttt
     7501 ctcagcaact atgccagatg ccatcaaacg tatcggtgtt cagtttatga aagcccctga
     7561 acatgtcaag attgcagcta aggaattgac aacagaattg gttgaccagt actatatccg
     7621 tgttaaggaa caagaaaaat ttgacaccat gactcgtctc atggatgtgg cacaaccaga
     7681 actcgctatt gtatttggtc gtaccaaacg ccgtgtggat gaattgactc gtggtttgaa
     7741 aattcgtggc ttccgtgcag aaggaattca tggcgaccta gaccaaaaca aacgtcttcg
     7801 tgtccttcgt gactttaaaa atggcaatct tgatgttttg gttgcgacag acgttgcagc
     7861 gcgtggtttg gatatttcag gtgtgaccca tgtctacaac tacgatattc cacaagatcc
     7921 tgagagttat gttcaccgta tcggtcgtac aggtcgtgct ggtaagtcag gtcaatctat
     7981 tacttttgtt gctccaaacg aaatgggtta ccttcaaatc attgaaaact tgactaagaa
     8041 acgcatgaaa ggtctcaaac ctgcaagtgt agaagaatcc ttccaatcaa aaaaacaggt
     8101 agctctcaag aaaatcgaac gtgattttgc agatgaaacc attcgtgcca actttgagaa
     8161 atttggtaag gatgctcgca aattggctgc tgagtttact ccagaagaat tggcaatgta
     8221 tatcttgagt ctgacagtcc aagacccaga tagccttcca gaagtggaga ttgcacgtga
     8281 aaaaccacta ccgtttaaac catcaggtaa tggtttcggt ggtaaagcta agggaggtcg
     8341 tggaggccgt cgtggggacg accgtcgaga gcgtgatcgc cgtggcaatg gtcgccgtga
     8401 tgagttcaaa aaaggaagtc gtggcaacga tcgttttgat aaggaaaaac gttaccgtaa
     8461 ggataataaa aaaccacgca atactttaag cgaaaagcaa acaggctttg ttattcgtaa
     8521 caaaggcgat aaataagaaa aagcggttca aaatgaatcg cttttttata taaggagacc
     8581 gaatgtgaaa agacaaataa aattattata ttatctttta taatgttatg atacacaaaa
     8641 ataggcaact tgtcaataga aaaggaacaa tttaattaaa aatattctgg tttttaatta
     8701 aattgtaagt caatgcaagt caataaggaa ttttcagata attattgaaa aagagacgtt
     8761 tttatgttat aatgatagcg attaaattcg aggtgaaaaa tggcacattt attagaaaaa
     8821 actagaaaaa ttacatcaat tttgaaacgc tcagaggagc agttgcagga tgagctccct
     8881 tacaacgcta ttacgcgtca gttagcggat attattcatt gcaatgcctg cattatcaat
     8941 agtaagggac gtctgcttgg ctattttatg cgttataaaa caaatacaga tcgcgtagag
     9001 caattcttcc aaactaagat tttcccagat gactacgttc aaggggctaa tatgatttac
     9061 gaaacagaag caaacttgcc tgttgagcat gatatgagta ttttccctgt tgagagtaga
     9121 gatgattttc cagatggctt gacgactatt gcaccgattc atgtatcggg gattcgcctt
     9181 ggttctttga ttatttggcg taatgataaa aaattcgaag atgaggactt ggttcttgtt
     9241 gagattgcca gtaccgttgt tgggattcag cttcttaact tccaacgtga agaagatgag
     9301 aaaaatattc gtcgtcgtac tgctgtcacc atggcggtca ataccctttc ttactccgaa
     9361 ctccgtgctg tttcagcaat tttaggggaa ttaaatggaa atgaagggca gttgactgcg
     9421 tcagtgattg cagatcgtat cggaatcact cgctctgtga ttgtcaatgc tcttcgtaaa
     9481 cttgagtctg cggggattat tgaaagtcgc tcacttggaa tgaagggaac ctatcttaag
     9541 gtcttgattt cagatatttt tgaagaagtg aagaaaagag attactaatg acaaaggctt
     9601 taatttcgat tgattataca gaagattttg ttgctgatag tgggaagctg acagcaggtg
     9661 ctccagctca ggcgatttcg gatgccatta gcaaggtaac tcgattagct tttgaacgag
     9721 gagattatat cttctttact attgatgctc atgaagaaaa cgattgtttc catccggaaa
     9781 gtaagttatt tcctcctcat aatctgattg ggacgagtgg acggaattta tatggagatt
     9841 tggggatctt ttatcaagag catggttcag acagtcgtgt cttttggatg gataaacgcc
     9901 attactcagc tttttcaggg actgacctag atattcgttt gagagagcgt agagtgtcta
     9961 ccgttatctt aacaggtgtc ttgacggata tctgtgtcct acatacagct atagatgcct
    10021 ataatctagg atatgacatc gagattgtta aaccagctgt tgcttccatc tggcctgaaa
    10081 atcatcaatt tgccctaggt catttcaaaa atacactcgg agctaagtta gtagatgaaa
    10141 atctaaatga actttctgag taacctggtt ggtgaaaatg aaaaaatttg aaaaaaagtg
    10201 ttgacaagca tcccaaaagt tgatatacta gtaaagtaat cgacgcgggg atggcggaat
    10261 tggcagacgc gcaggactaa ggatcctgtg accgctttag gtcgtgaggg ttcaagtccc
    10321 tctctccgca taggaaaaga gttagctaaa gctagctctt ttgtatttct ctaaaaaatt
    10381 agaatccagt caaatgtagt ggagaatcag cgaaaatatg ggaaaactga tccttgtttc
    10441 tgcttttgtc atagaagaat aataaagatt atcttcttca aattctccga tattctctaa
    10501 agttttgtgc aagttgcaca gaactttttt atttttttgg tcatcttgcc atagaaatat
    10561 aaagcgtttt catctataat ataattatca aaagacaaaa ggagttcacc tcatggtaga
    10621 attgaatctt aaaaatattt acaaaaaata tccaaacagc gaacactatt cagttgaaga
    10681 tttcaacttg aacatcaaag ataaagaatt tatcgttttc gtaggacctt caggatgtgg
    10741 taaatcaact acactccgta tgattgctgg tcttgaagac attacagaag gtactgcatc
    10801 tatcgatggc gtggttgtca acgacgtagc tccaaaagac cgtgatatcg ccatggtatt
    10861 ccaaaactac gctctttacc cacacatgac tgtttatgac aacatggctt tcggtttgaa
    10921 attgcgtaaa tacagcaaag aagacattaa caaacgtgtt caagaagcag ctgaaatact
    10981 tggattgaaa gaattcttgg aacgtaaacc agctgacctt tcaggtggtc aacgtcaacg
    11041 tgttgccatg gggcgtgcga ttgtccgtga tgcgaaagta ttcttgatgg acgaaccttt
    11101 gtcaaacttg gatgccaaac ttcgtgtatc aatgcgtgct gaaatcgcta aaattcaccg
    11161 tcgtatcgga gctacaacta tctatgtaac tcacgaccaa acagaagcga tgacacttgc
    11221 agaccgtatc gttattatgt cagctactaa gaaccctgct ggtacaggta ctatcggacg
    11281 tgtagaacaa atcggcactc ctcaagaagt ttacaaaaat ccagttaaca aattcgttgc
    11341 aggattcatc ggaagcccag ctatgaactt catcaacgtg aaattggttg gtagcgaaat
    11401 tgtttctgac ggtttccgtt tgaaagtgcc agaaggagca ttgaaagttc ttcgtgaaaa
    11461 aggctacgaa ggaaaagaat tgatctttgg tatccgtcca gaagacgtga atgcagaacc
    11521 tgctttcctt gaaacattcc cagactgtgt tgtaaaagcg actatctctg tatcagaact
    11581 gcttggttca gaatctcacc tttactgcca agttggtaaa gacgagtttg ttgcaaaagt
    11641 tgatgctcgt gactacttgc aaacaggtgc aacagttgag cttggatttg acttgaacaa
    11701 agcacacttc ttcgatgtag aaactgaaaa aacaatctac taaaataaat aaaattcaaa
    11761 gcactacaag aaaagatatt tctttatcaa ttgtagtgga gagatatcag ttaatctagg
    11821 gagagaaaca aaatgcttct ctcctttttg ctagggaagt catattatgc atctatattg
    11881 tgatgctctt taatactctt cgaaaatctc ttcaaaccac gtcaacgtcg ccttgccgta
    11941 cgtatgatta ctgacttcgt cagttttatc tgcaacctca aagctgtact ttgagcagtt
    12001 tacggctagt ttcctagttt gctctttgat ttccattgag tattatttgt gggtaccatc
    12061 tacaagtgaa gctatatgcg taaactacgt gagcaattga attcgaacta gagaggtaat
    12121 aataaattta tgctatagtt atggtgactt gtatgctttt gattctagtt tatcaaataa
    12181 tagattagaa ttgtcagata atatcatttt gtgttataat gaagaaaaaa cagaggtgtt
    12241 caaatgtcag aagcaggtca taagttttta gcaaaattgg ggaaaaaacg cttacgtcca
    12301 ggtggaaagc gtgccacaga ttggttaatt gcagaaggag gattttcaaa agaaaagaga
    12361 atactagagg ttgcgtgtaa taggggaact acagcaattg agttggcaca gcgttttggt
    12421 tgcaagataa ctgctgttga tatggatgct caagctttag aagtggctaa aaaatctgct
    12481 ggaacggcag gtgttgctca tttaatcagt tttgaaagag caaatgcaat gaaacttcct
    12541 tatcaagatg ctagttttga tattgttata aatgaagcta tgctgactat gcaagccgat
    12601 caagctaaga aaaaatgtgt aatggaatat ctaagggtat taaaacctgg aggtcttctc
    12661 ttgacacatg atgtacttct taaggaagct aaagagtcta tcagacagga attatcacaa
    12721 gcaattcatg taaatgtagg tcctttaact caagatggtt gggaacaggt gatgatagaa
    12781 tcaggttatt gtgatgtgaa agcattgact ggtgaaatga cattaatgaa attatcgggt
    12841 atgatttatg acgaaggttt gctaggaact ttgaaaattt gtgtaaatgc ttgtaaaaag
    12901 gagaatagaa agcagttttt aactatgtat aaaatgtttg ctaagaataa acagaaattg
    12961 ggctttattg cgatggctag ttataaatcg tcaaaacgtt agataattat tgaagttaac
    13021 ttttcctttt ttctttctta aaaaatatgc tataatagag agtaaaaaac tttgaaagaa
    13081 agaaaaagat gaatttaaaa gattacattg caacaattga aaattatcca aaggaaggca
    13141 ttaccttccg tgatattagt cctttgatgg ctgatggaaa tgcttatagc tacgctgttc
    13201 gtgaaatcgt tcagtatgct actgacaaga aagtcgacat gatcgtggga cctgaagctc
    13261 gtggatttat cgtgggttgt ccagttgcct ttgagttggg aattggtttt gcgcctgttc
    13321 gtaagccagg taaattgcca cgcgaagtta tttctgctga ctatgaaaaa gagtacggtg
    13381 tcgatacctt gactatgcac gcggatgcca ttaagccagg tcaacgtgtt cttattgtag
    13441 atgacctttt ggcgacaggt ggaactgtta aggcaactat cgagatgatt gaaaaacttg
    13501 gtggtgttat ggcaggttgt gccttccttg ttgaattgga tgaattgaac ggccgtgaaa
    13561 aaattggtga ctacgactac aaagttctta tgcattatta atgaaaacag tccctagggc
    13621 tgttttctct acactaggat ataaaaatag actataacta gttagagaaa aactataatt
    13681 gaaaactata tcttcttaca gtataataaa aggactaagt gtttgagatt tgtcttcaaa
    13741 catatgcaat tattcctgaa agagtacagt taggagaggg ttatgccgat tcgaattgat
    13801 aaaaaattgc cagctgttga gattttacgg acagagaata tctttgtcat ggatgatcaa
    13861 cgtgctgccc accaagatat ccgtcctttg aagattttaa ttttaaatct catgccacag
    13921 aaaatggtca cagagaccca gttgttgcgc cacttggcta atacacccct acaactggat
    13981 attgattttc tctatatgga gagccaccgt tctaaaacaa ctcgttcaga gcacatggag
    14041 accttctata aaacttttcc tgaagtcaag gatgagtatt ttgatgggat gatcatcacg
    14101 ggtgctccag ttgagcattt accatttgag gaagtggact attgggagga atttagacag
    14161 atgcttgagt ggtctaagac tcatgtctat tcgacccttc atatctgttg gggggctcag
    14221 gctgggcttt atctgcgcta tggtgtagaa aaataccaga tggacagtaa gctatcaggt
    14281 atttatcctc aggacaccct aaaagagggt caccttctat ttagaggctt tgatgatagc
    14341 tatgtatccc ctcattcacg gcacacggag atttctaagg aagaggtctt aaacaagacc
    14401 aatctcgaga ttttatcaga aggacctcag gttggggttt ctattttggc cagtcgtgat
    14461 ttacgagaaa tttatagttt tggtcatttg gagtatgacc gtgatacctt ggcaaaagag
    14521 tattttcgag atcgtgatgc aggtttcgac ccacatattc cagaaaatta ctttaaagat
    14581 gatgatgtta atcaggtacc ttgtctttgt tggtcttcat ctgcagccct ctttttcagt
    14641 aattgggtaa accatgcggt ctatcaggag acgccttttg attggagaaa gatagaagat
    14701 gatgcatctg catatgggta tttataagag gaattatgac atatttagac gcttttaaat
    14761 caggtatctt ggttttaccg agtgccctgc tcttgcattt taaggaactc tttccttcta
    14821 gcgacgattt tctggtttgg caatttttct atttgcaaaa tacgacaggc ttagaagaaa
    14881 tgtcgccaag ccagattgct gaaaggattg gcaaggaaat ttcggatgtc aaccagtcca
    14941 tttctaatct gacggaaagg ggactgctcc agtatcgtac tatcgaatta aatggcgaaa
    15001 ttgaattgct ctttgatgct agtttggcct tggaacgttt ggatgacctg tttggagcag
    15061 ttcattcaag ttcagaccag ctgacacctc aaaaccagct caaggatttg gtggaaacct
    15121 tccagcagga gttgggacga ttgttgacgc cttttgagat tgaggatttg accaagacac
    15181 taaaggaaga tggaaccagt gctgacttga ttaaggaggc tcttcgtgaa gctgttttga
    15241 atggaaaacc aaactggaag tacattcagg cgattttgag aaactggcgc catgaaggaa
    15301 tcaagagtgt ggctcaaatt gaggccaaga gagcagaaag agaagcaagc aatcctcagt
    15361 tgacacaggt atctgcagat ttcataaatg ccatggatct ctggaaggat taatccatgc
    15421 aagtaggctt gaaatccgag taagatttgc aagctgtgta taattgtgat agaataaata
    15481 gaaaataaat tgaaaaaaga ggtatgtgaa atgtcacgta aaccatttat cgctggtaac
    15541 tggaaaatga acaaaaatcc agaagaagct aaagcattcg ttgaagcagt tgcatcaaaa
    15601 cttccttcat cagatctggt tgaagcaggt atcgctgctc cagctcttga tttgacaact
    15661 gttcttgctg ttgcaaaagg ctcaaacctt aaagttgctg ctcaaaactg ctattttgaa
    15721 aatgcaggtg ctttcactgg tgaaactagc ccacaagttt tgaaagaaat cggtactgac
    15781 tacgttgtta tcggtcactc agaacgccgt gactacttcc atgaaactga cgaagatatc
    15841 aacaaaaaag caaaagcaat ctttgcgaac ggtatgcttc caatcatctg ttgtggtgaa
    15901 tcacttgaaa cttacgaagc tggtaaagct gctgaattcg taggtgctca agtatctgct
    15961 gcattggctg gattgactgc tgaacaagtt gctgcctcag ttatcgctta tgagccaatc
    16021 tgggctatcg gtactggtaa atcagcttca caagacgatg cacaaaaaat gtgtaaagtt
    16081 gttcgtgacg ttgtagctgc tgactttggt caagaagtcg cagacaaagt tcgtgttcaa
    16141 tacggtggtt ctgttaaacc tgaaaatgtt gcttcataca tggcttgccc agacgttgac
    16201 ggtgcccttg taggtggtgc gtcacttgaa gctgaaagct tcttggcttt gcttgacttt
    16261 gtaaaataat cagtaagtag caaaagctag gtggaacagc attcagatgt ctgttacatt
    16321 ttttatagga gaggaaagat tgaaaacaaa aattggatta gcaagtatct gtttactagg
    16381 cttggcaact agtcatgtcg ctgcaaatga aactgaagta gcaaaaactt cgcaggatac
    16441 aacgacagct tcaagtagtt cagagcaaaa tcagtcttct aataaaacgc aaacgagcac
    16501 agaagtacag actaatgctg ctgcccactg ggatggggat tattatgtaa aggatgatgg
    16561 ctctaaagct caaagtgaat ggatttttga caactactat aaggcctggt tttatattaa
    16621 ttcagatggt cgttactcgc agaatgaatg gcatggaaat tactacctga aatcaggtgg
    16681 atatatggcc caaaacgagt ggatctatga cagtaattac aagagttggt tttatctcaa
    16741 gtcagatggg gcttatgctc atcaagaatg gcaattgatt ggaaataagt ggtactactt
    16801 caagaagtgg ggttacatgg ctaaaagcca atggcaagga agttatttct tgaatggtca
    16861 aggagctatg atgcaaaatg aatggctcta tgatccagcc tattctgctt atttttatct
    16921 aaaatccgat ggaacttatg ctaaccaaga gtggcaaaaa gtgggcggca aatggtacta
    16981 tttcaagaag tggggctata tggctcggaa tgagtggcaa ggcaactact atttgactgg
    17041 aagtggtgct atggcgactg acgaagtgat tatggatggt gctcgctata tctttgcggc
    17101 ctctggtgag ctcaaagaaa agaaagattt gaatgtcggc tgggttcaca gagatggtaa
    17161 acgctatttc tttaataata gagaagaaca agtgggaacc gaacatgcta agaaagtcat
    17221 tgatattagt gagcacaatg gtcgtatcaa tgattggaaa aaggttattg atgagaacga
    17281 ggtggatggt gtcattgttc gtctaggtta tagcggtaaa gaagacaagg aattggcgca
    17341 taacattaag gagttaaacc gtctgggaat tccttatggt gtctatctct atacctatgc
    17401 tgaaaatgag accgatgctg agaatgacgc taaacagacc attgaactta taaagaaata
    17461 caatatgaac ctgtcttacc ctatctatta tgatgttgag aactgggaat atgtaaataa
    17521 gagcaagaga gctccaagtg atacagacac ttgggttaaa atcatcaaca agtacatgga
    17581 cacgatgaag caggcgggtt atcaaaatgt gtatgtctat agctatcgta gtttattaca
    17641 gacgcgttta aaacacccag atattttaaa acatgtaaac tgggtagcgg cctatacgaa
    17701 tgctttagaa tgggaaaacc cttattattc aggagaaaaa ggttggcaat atacctcttc
    17761 tgaatacatg aaaggaatcc aagggcgcgt agatgtcagc gtttggtatt aagcgatgat
    17821 ttgaaagagg gatgtgatag tagcaccctc tttttctttg ttttatgata gttcatcctc
    17881 gagtaaattc aagatcttgc tcggaaatga agcttatata gtagattgaa tatagacaaa
    17941 gaccttgtga ttggtaaaac attttagaaa ttcatttacc tttcctaatc gacttggttt
    18001 catcttattt caatctatta tagtattggg gaatttcttc aaaccacatc agcttggtca
    18061 gttctacctg cgacctcaaa acttgtgctt tggtcaagct gggtttagtt tcctagtttg
    18121 ctgatggatt tccattgact ataagcatcc aaccctcttt tttgtcttct aaagaattct
    18181 taaattatca gtctattgca acttttctca tataagttct ttgtcttgct attagttttc
    18241 cttagtagta tactaaggta gtaatcatta agaagtggtt acaaaaaata atgaatgagg
    18301 taaagaaaat ggtagaattg aaaaaagaag cagtaaaaga cgtaacatca ttgacaaaag
    18361 cagcgccagt agcattggca aaaacaaagg aagtcttgaa ccaagctgtt gctgatttgt
    18421 atgtagctca cgttgctttg caccaagtgc actggtatat gcatggtcgt ggtttccttg
    18481 tatggcatcc aaaaatggat gagtacatgg aagctcttga cggtcaattg gatgaaatca
    18541 gcgaacgctt gattacactc ggtggaagcc cattctctac attgacagag ttccttcaaa
    18601 atagtgaaat cgaagaagaa gctagtgaat accgtaatgt tgaagaaagc ttggaacgtg
    18661 ttcttgttat ctaccgttac ttgtcagaac ttttccaaaa aggtttggat gtcactgatg
    18721 aagaaggtga cgatgtgaca aacggtatct ttgcaggcgc taaaactgaa acagataaaa
    18781 caatttggat gcttgcagcc gaacttggac aagcacctgg tttgtaagaa ataaggaaac
    18841 atagataaat ctgtaggaaa tttcttacag attttttcta ttttgtaagc tattccaaac
    18901 cagtcttttt ttgagttttt tgataaaata gtactatcag tgaaaaggat ggaagtatga
    18961 ctaagaaaat cgtagctatt tgggcccagg atgaagaggg tgtgattggt aaggaaaatc
    19021 gtctgccttg gcatttgcca gcagaattgc agcactttaa agaaacaact ctgaatcatg
    19081 ctatcttgat ggggcgtgtg acctttgatg ggatggggcg tcgcttgctt ccaaaacggg
    19141 aaaccttgat tttgacgcgt aatccggaag aaaagataga tggggttgct acttttcagg
    19201 acgtccagtc tgttcttgac tggtatcagg ctcaagaaaa gaatctctac attattggtg
    19261 ggaagcaaat ttttcaggct tttgaacctt accttgatga agtgattgtc actcacattc
    19321 atgctcgtgt ggaaggagat acctatttcc ctgaagaact tgacttgtct ctttttgaga
    19381 cagtttcaag caaattttac gccaaagatg agaagaatcc ttatgatttt accatccaat
    19441 accgcaagag aaaggaactc taatggaacg gagtttattt ggtctattca cggcttttct
    19501 gtgtttcatc tgctttctgg caggagcaca agcttttcgt aaaaagcgtt acgggctttc
    19561 tatcctcctt tggttaaatg cctttaccaa tctggtcaat agtattcatg ctttttacat
    19621 gaccttattt tagatagaat gacagtatag aatggaagga aatcatgtct acaaatagaa
    19681 aaaatgatat gatggtttat tgctcatttt gtggcaaaaa ccaagaagaa gtacaaaaaa
    19741 taattgctgg caacaatgct tttatttgta atgaatgcgt ggagttagct caggaaatca
    19801 ttcgagaaga attggttgag gaagtcttgg cagacttgtc tgaggtgcca aaaccaattg
    19861 aactcctcca tatcttgaac cactatgtaa ttggtcaaga tcgtgccaag cgtgccttgg
    19921 cagtggcggt ttataaccac tacaaacgca tcaatttcca cgatacacgc gaagagtcag
    19981 aagatgtaga tttgcagaag tcaaacattt tgatgattgg cccaactggt tcagggaaaa
    20041 ctttccttgc ccagaccttg gctaagagct tgaatgtgcc ttttgctatt gcggatgcga
    20101 cagctctgac ggaggctggt tatgtgggtg aggatgtgga aaatatcctc ctcaaactct
    20161 tgcaggttgc tgactttaac atcgaacgtg cagagcgtgg cattatctat gtggatgaaa
    20221 ttgacaagat tgccaagaag agtgagaatg tgtctatcac acgtgatgtt tctggtgaag
    20281 gggtgcaaca agcccttctc aagattatcg agggaactgt tgctagcgtg ccgcctcaag
    20341 gtggacgcaa acatccacaa caagagatga ttcaagtgga tacaaaaaat atcctcttca
    20401 tcgtgggtgg tgcttttgat ggtattgaag aaattgtcaa acaacgtctg ggtgaaaaag
    20461 tcatcggatt tggtcaaaac aataaggcga ttgatgaaaa cagctcatat atgcaagaaa
    20521 tcatcgctga agacattcaa aaatttggta ttatccctga gttgattgga cgcttgcctg
    20581 tttttgcggc tcttgagcaa ttgaccgttg atgacttggt tcgcatcttg aaagagccaa
    20641 gaaatgcctt ggtgaaacaa taccaaacct tgctttctta tgatgatgtt gagttggaat
    20701 ttgacgacga agcccttcaa gagattgcta ataaagcaat cgaacggaag acaggggcgc
    20761 gtggacttcg ctccatcatc gaagaaacca tgctagatgt tatgtttgag gtgccgagtc
    20821 aggaaaatgt gaaattggtt cgcatcacta aagaaactgt cgatggaacg gataaaccga
    20881 tcctagaaac agcctagagg tgactatgga acttaatata cacaatgctg aaatcttgct
    20941 cagtgcagct aataagtccc actatccgca ggatgaactg ccagagattg ccctagcagg
    21001 gcgttcaaat gttggtaaat ccagctttat caacactatg ttgaaccgta agaatctcgc
    21061 ccgtacatca ggaaaacctg gtaaaaccca gctcctgaac ttttttaaca ttgatgacaa
    21121 gatgcgcttt gtggatgtgc ctggttatgg ctatgctcgt gtttctaaaa aggaacgtga
    21181 aaagtggggg tgcatgattg aggagtactt aacgactcgg gaaaatctcc gtgcggttgt
    21241 cagtctagtt gaccttcgtc atgacccgtc agcagatgat gtgcagatgt acgaatttct
    21301 caagtattat gagattccag tcatcattgt ggcgaccaag gcggacaaga ttcctcgtgg
    21361 taaatggaac aagcatgaat cagcaatcaa aaagaaatta aactttgacc caagtgacga
    21421 tttcatcctc ttttcatctg tcagcaaggc agggatggat gaggcttggg atgcaatctt
    21481 agaaaaattg tgaggaaaag aaaatggcaa aaacaattca tacagataag gccccaaagg
    21541 ctatcggacc ctatgttcaa ggaaaaatcg ttggcaacct tttgtttgct agcggtcaag
    21601 ttcccctatc ccctgaaact ggggaaattg taggagagaa tatccaagaa cagacagagc
    21661 aagtcttgaa aaacatcggt gctattttgg cagaagcagg aacagacttt gaccatgttg
    21721 tcaaaacaac ttgcttcttg agcgatatga acgactttgt tccttttaat gaggtttacc
    21781 aaacggcctt caaagaggaa ttcccagctc gttcagctgt ggaggtagct cgtcttcctc
    21841 gtgatgtaaa agtcgaaatt gaagtcatcg cagagattgg ataagctagt tgaagtttgg
    21901 tgttgccaaa cttcttttga tataaggaga aaaagatgac aaagaaacaa cttcacttgg
    21961 tgattgtgac agggatgagt ggcgcaggga aaactgtagc cattcagtcc ttcgaggatc
    22021 taggttattt caccattgat aatatgccgc cagctctctt gcctaagttt ttgcagctgg
    22081 ttgaaattaa ggaagacaat cctaagttgg ccttggtagt ggatatgcgt agccgttctt
    22141 tcttttcaga gattcaagct gttttggatg agttggaaaa tcaagatggt ttggatttca
    22201 aaatcctctt tttggatgcg gctgataagg aattggtcgc tcgttacaag gaaaccagac
    22261 ggagtcaccc actagcagca gacggtcgta ttttagatgg aatcaagttg gaacgtgaac
    22321 tcttggcacc tttgaaaaat atgagccaaa atgtggtgga tacgactgaa ctcactccac
    22381 gtgagctgcg caaaaccctt gcagagcagt tttcagacca agaacaagcc cagtctttcc
    22441 gtatcgaagt catgtctttc ggatttaagt atggaatccc gattgatgcg gacttggtct
    22501 ttgatgtccg tttcttgcca aatccctatt atctaccaga actgagaaac caaacgggtg
    22561 tggatgaacc tgtttatgat tatgtcatga accatcctga gtcagaagac ttttatcaac
    22621 atttattggc cttgattgag ccgattctgc caagttacca aaaggaaggt aagtccgttt
    22681 tgaccattgc catgggatgt acgggtggac aacaccgtag tgtggcattt gctaaacgct
    22741 tggcgcagga cttatccaag aattggtctg ttaatgaagg gcatcgcgac aaagaccgca
    22801 gaaaggaaac ggtaaaccgt tcatgagaaa accaaagata acggtgattg gtggagggac
    22861 tggaattccc gtcattctaa aaagtctgcg ggaaaaagat gtggaaatcg cagctatcgt
    22921 gacggtggca gatgatggtg gttcttcagg tgaactccga aaaaatatgc aacagttgac
    22981 accgccaggt gatcttcgta atgtccttgt ggccatgtcg gatatgccta agttttatga
    23041 gaaggtcttt cagtatcggt tctctgagga tgccggagcc tttgctggcc atccattggg
    23101 aaatctcatc attgctggct tgtcagaaat gcagggttca acctataatg ccatgcagtt
    23161 attgagcaaa tttttccata caacagggaa aatttatcct tccagtgacc atcctttgac
    23221 ccttcatgca gtctttcagg atgggacaga agtggttgga gagagtcata ttgtagacca
    23281 tcgaggcata attgacaatg tctatgtgac taatgctcta aacgatgata cgcctctggc
    23341 cagccgtcga gtagtgcaga ccatccttga aagtgacatg attgtcctag ggccaggttc
    23401 cctctttacc tctattttgc ccaatatcgt gattaaggaa attgggcggg ctcttttgga
    23461 aaccaaggca gaaattgcct atgtctgcaa tattatgacc caacgtggag agacggaaca
    23521 ctttacagat agcgaccacg tggaagtctt gcatcgtcac cttggtcgcc cttttatcga
    23581 cactgtcttg gtgaatattg aaaaagtgcc tcagaaatac atgaattcca accgttttga
    23641 tgaatacttg gtgcaagtgg aacacgattt tgtaggtctt tgtaagcaag tttcgcgcgt
    23701 gatttcatct aacttccttc gtctggaaaa tggcggtgcc ttccacgatg gagatttgat
    23761 tgtggacgag ttgatgcgca ttatacaggt gaaaaaatga gtttcacagt agcagtaaaa
    23821 gaagaaatcc tgggccaaca ccatctgagc cggcatgaat tatctgccat tatcaaaatg
    23881 tctggtagca tcggtctctc gacttcgggc ttgactttgt cagttgtgac agaaaatgcc
    23941 aaactggccc gtcacctcta tgagtccttt ctccatttct atgaaatcaa atctgaaatt
    24001 cgtcaccacc aacggagcaa tcttcgcaag aatcgcgtct ataccgtttt tacagatgaa
    24061 aaggtgcagg atttgttaag tgatttgcac ttggcagact ctttctttgg cttggaaaca
    24121 ggtattgatg aggcgatttt atcggatgag gaagcaggtc gtgcctatct ctgtggcgct
    24181 ttcttggcaa atgggagcat tcgtgaccct gagtcaggca agtaccagtt ggaaatcagt
    24241 tctgtttatc tggaccacgc gcaaggaatt gcctcccttc tccaacagtt tttactggat
    24301 gccaaggtgc ttgagcgcaa gaagggggct gtgacctatc tccagcgagc agaagacatt
    24361 atggacttct tgatagtaat cggagccatg caggcacgtg atgattttga gcgggttaag
    24421 attttgcgag aaactcgtaa cgacctcaat cgggctaata atgccgagac agctaatatc
    24481 gctcggacag tttctgccag catgaagacc attaacaata tcagtaaaat caaagatatc
    24541 atgggcttag aaaatttgcc agtagatttg caggaagtgg cgcaactgag gattcagcac
    24601 ccagactact ctatccagca gttagcagat agcctcagca cccctctgac caaaagtggt
    24661 gtcaatcaca gactcagaaa aatcaataag atagccgatg aattataaaa ccacgaatcc
    24721 tatgtgactc gtggttcttt tttataaact agtagagtgt tttggttgta ctttttgttc
    24781 agggtcaatg tagttgatag cgttgttgac agcagttgga gcttctccga ggcctgtcgc
    24841 aatcagatca atttttccgt catagtagca gcagtcaccg atagcataga tacctgcttg
    24901 gctggattcc tgtttgctgt tgacgataat cttgtgacgg ttgaggtcga gcccccagtt
    24961 tttaaggtta ccgacagaag atttgaaacc atagttgaca aagaggtggt ctaggtcaat
    25021 ggtttcagtt tcatcagatt tgacttttgt gatttcaagt ttatcaagtg tttttccatt
    25081 tccaaggagt tggctagggg cgaatggtgt cttgatggtt acagatgatt cttgcaaggc
    25141 ttgaacactg tgttccaagg cacggaaatt atctctgcgg tgaacaaggg tagttggtgc
    25201 gattttttca aaagccaaag cccaatccac agccgagtct cccccaccaa gaatcgtcac
    25261 tttcttacca gcgtattgct gaatgttaga aacgtggtag tggatatttt catagccctc
    25321 aaccccttca agttccagcg gacgtggttt gaaggcaccg ccacccatag cgatgatgac
    25381 tgttttagtc aagtgacttc ctttagaagt tgtgatggca aattcttctt cttgtttgtc
    25441 aatctcaaga accgtttcat tgagatgaat aggggtatca aatccattta gctgttcaat
    25501 caagcggtta gtcaactctt ctccagtcag gtttgggaag cctggtacgt ctaggatttc
    25561 cttttcaggg tagagaatag caggttgtcc acctagctgg ggaagagagt cgatgatttg
    25621 aaccttggct tggcgtaggt gggcataaaa ggctgcaaaa agcccgacag gaccaccacc
    25681 cacaatggta atatcataga gttgagacat agtttctcct ttgttttttc tagtcagttt
    25741 attttatcat atttttgttc aatgtaaact ggactaggat agggaaaaaa atgccagaaa
    25801 atggtataat aggacgaaag tgttttgata gggaggatat cagggatgaa ttttcaacaa
    25861 ttatccaatc tgcaatattg gaccagtttg tttgcaagtc catggacgat agctatcaat
    25921 ctgattgata ttttgattgt tgcttatatt ttataccatt ttacaaaagc tattgcagga
    25981 accaagatta tgattttggt acgtggagtt ttggtgttta ttttagctca aatccttgca
    26041 aatatgattg gtttgactac gatttcttgg ttaatcaatc aaattattac ttatggggtt
    26101 attgcggcgg ttgttatctt ctctccagag attcggactg gtttggaacg tttgggaaga
    26161 gcgacagatt tcttttccaa tgcccctatt agtgctgagg aacagatgat tcgtgccttt
    26221 gttaagtctg ttgaatacat gagtcctcgt aaaatcgggg ccttggttgc tattcagcgt
    26281 gtacgtacct tgcaggagta tatttcgaca ggaattccct tggatgctaa gatttctgca
    26341 gaacttctca ttaacatttt tattcccaac actcccctac atgatggtgc ggtgattatc
    26401 aaagaagaac gtatcgctgt gacgtctgcc tatctgccct tgacaaaaaa cacagggatt
    26461 tccaaggaat ttgggaccag acaccgggcg gctatcggtt tatcagaagt ctcagatgcc
    26521 ttgacttttg ttgtatcaga ggaaacggga ggaatttcga taacctataa tggaaggttt
    26581 aagcacaacc taacacttga tgaatttgaa acagaattac gtgaaatctt acttccaaaa
    26641 gaggaagtgg gtcttagttt taaagaacga ttgctaggag gatggaaaca tgaaaaaaaa
    26701 tagtttatat atcatatcct cactcttttt tgcttgtgtc ttatttgtct atgctacggc
    26761 gacgaatttt caaaacagta ccagtgctag gcaggtaaaa acggaaacct atactaatac
    26821 agtaacaaat gtccctattg acatacgcta taatagtgat aagtatttta ttagcggttt
    26881 tgcttcagaa gtatcagtgg tcttgactgg tgcaaatcgc ctatcgctag ctagtgaaat
    26941 gcaagaaagt acacgtaaat tcaaggttac tgctgaccta acagatgccg gtgttggaac
    27001 gattgaagtt cctttgagca ttgaagattt acccaatggg ctgaccgctg tggcgactcc
    27061 gcaaaaaatt acagtcaaga ttggtaagaa ggctcagaag gataaggtaa agattgtacc
    27121 agagattgac cctagtcaaa ttgatagtcg ggtacaaatt gaaaatgtca tggtgtcaga
    27181 taaagaagtg tctattacga gtgaccaaga gacattggat agaattgata agattatcgc
    27241 tgtcttgcca actagcgaac gcataacagg taattacagt ggttcagtac ctttgcaggc
    27301 aatcgaccgc aatggtgttg tcttaccggc agttatcact ccgtttgata caataatgaa
    27361 ggtgactaca aaaccagtag caccaagttc aagcacatca aattcaagta caagcagttc
    27421 atcggagaca tcttcgtcaa cgaaagcaac tagttcaaaa acgaattaaa aatagaaaag
    27481 gattttataa caatgggtaa atattttggg actgatggag tccgtggaga agctaaccta
    27541 gaactaacac cagaattagc ctttaaacta ggacgttttg gaggctatgt tcttagtcaa
    27601 catgaaacgg aagcgccgaa agtctttgta ggacgtgaca cacgtatttc aggggaaatg
    27661 ctggaatcgg ccttggtggc aggtctcctt tcagtaggga ttcacgtata caaacttggt
    27721 gtccttgcaa caccagcagt agcttacttg gttgaaactg aaggagcaag tgccggtgtc
    27781 atgatttctg ctagccacaa cccagccctt gataacggaa tcaagttctt tggcggtgat
    27841 ggcttcaaac tagatgatga aaaagaagca gaaattgaag ccttgctaga tgctgaggaa
    27901 gacactcttc ctcgtccaag tgcagaaggc ttaggaatct tggtagatta tccagaaggc
    27961 ttgcgtaagt atgaaggata ccttgtttca actggaactc ctcttgatgg aatgaaggtt
    28021 gccttggata cagctaatgg agcagcttct accagtgccc gtcaaatctt tgcagacctt
    28081 ggtgcccaat tgacggttat cggggaaaca ccagacggtc ttaacatcaa ccttaatgtt
    28141 ggttcaacac atccagaagc ccttcaagaa gtggtcaaag aaagtgggtc agctattggt
    28201 ttggcctttg atggagacag tgaccgcttg attgctgttg atgagaatgg tgacatcgtc
    28261 gatggtgaca agattatgta catcatcgga aaataccttt ctgaaaaagg acaattggct
    28321 caaaatacaa ttgtgacaac tgttatgtct aaccttggtt tccacaaggc cttgaatcgc
    28381 gaaggtatta acaaggcagt tactgcagtt ggtgaccgct acgttgttga agaaatgaga
    28441 aaatcaggct acaaccttgg tggtgaacag tctggtcacg ttatcttgat ggattacaat
    28501 accacaggtg atggtcaatt atcagcagtt caattgacta aaatcatgaa ggaaactggt
    28561 aagagcttat cagagttggc ggcagaagta acgatttatc cacaaaaatt agttaatatc
    28621 cgagtggaaa acgtcatgaa ggaaaaggcc atggaagtgc cagctatcaa ggccatcatc
    28681 gagaagatgg aagaagaaat ggcggggaac ggccgtatcc ttgttcgtcc aagtggaaca
    28741 gagcccctct tgcgtgttat ggcagaagcg cctacaacag aagaagtaga ctactatgtt
    28801 gataccatca cagatgtagt tcgtgctgaa attgggattg attaaatcct agaaaactag
    28861 atgaaaataa aaaattatgg tacaatagct tataatattg tagccgatgg agaaagacat
    28921 gaatcttaaa cgtgaacaag aatttgttag tcagtatcat tttgatgcgc gtaattttga
    28981 atgggaaaat gaaaatggag ctcctgaaac taaggtggat gtgaatttcc aattgctcca
    29041 acatgaccaa gaaaatcaag tgacttcctt gattgtcatc ttgagcttta tgattgtatt
    29101 tgataagttt gtcattagtg gaacaatctc acaagtaaac catatcgatg gccgtattgt
    29161 caatgaacca aatgaattga accaagaaga ggtggaaacc ttggctcgtc catgtttgaa
    29221 catgctcaat cgtttgactt acgaagtgac tgaaattgca ttagacttac caggaattaa
    29281 tttggagttc taatatgaaa ttagctgttt tcacagattc ctctgcttat ctcagtgcag
    29341 agaccttgca aagagaagac ttgtttgtct tggatattcc tgtcaatatt gatggtgagg
    29401 aatatgtcga aggcatcaat ctgtctgctg aggaatttta ccaaaaaatg gctcaggctt
    29461 ctgaattgcc taagaccagt caaccaagta ttgccaagtt agatgaaatc ttaacttcgc
    29521 tcaaagaaca aggctataca catgccttgg ggcttttcct atcttctgga atttcaggtt
    29581 tttaccaaaa tatccagtat atggtcgatg actatgaggg cttaaccatt gctttcccag
    29641 acactttgat tacaagtgct cccctaggca tcatggttga aagcgtcttt aattggcgtg
    29701 atcagggcga tgattttgcc agcattcagg ataagctagc cattcaaatc agccgtacgt
    29761 cagcctttat catggtagat gacttggatc atttggtaaa aggtggacgt ctttcaaatg
    29821 gggctgccat tttgggcaat ttgcttagca ttaagccaat cctttatttt aacgatcaag
    29881 gtgtgattga agtttacgaa aaagttcgta ctgaaaagaa ggccaccaag cgcttaattg
    29941 aaattatcaa ggaaacaacg gcttcaggtc aataccgggt cattgtcatt catgggaatg
    30001 ctcctgaaaa ggctgaagaa ttgcgtcagc atttgcttga ttttggcttg ggttcggatg
    30061 tttcacttgc tacatttggt agtgtcattg gaacgcacct aggagcagga agtattgctc
    30121 tgggttatat tccagtgatt tagttggcgc ttctaggcta gttaagatgc cttgtatctt
    30181 agcaattgaa cacggactac ctgcttcttg aaaaaagatg cctgtcttgc ctttcgtgga
    30241 aagtcagtgc cattccctat ttttcagggg cagctaaggt cctttgtatc ttgataattg
    30301 aacacgcctg gaaccctgtg tgaaaaagat agttcttcca agaagtagac acttcttgat
    30361 tggaattcct attttcactt tgtgttctta cggactttgt atcttagaca ggagtagaga
    30421 tgagtattcg agtaattatt gccggtttta agggaaagat gggccaggct gcttgtcaga
    30481 tggtattgac tgatccagac ttggacttgg tagcagtttt ggatcctttt gagtctgagt
    30541 cagaatggca gggtattcct gttttcaagg ataaggctga tttagcttgt tttgaagcgg
    30601 atgtctgggt agattttact actccagctg ttgcctacga aaatacacgt tttgctcttg
    30661 aaaatggctt tgctccagta gttggaacga ctggtttcac gagtgaagaa attgcagagc
    30721 taaaagaatt ttctcgtacc caagacttgg gtggcctgat tgcccctaac tttgccttgg
    30781 gtgctgtctt actcatgcaa tttgcgacgc aggctgccaa atatttccca aatgtggaga
    30841 ttattgagct ccatcatgac aagaaaaagg atgctccgag tggaacagcc attaaaacag
    30901 ctgagttgat ggcagagatt cgagagtcaa ttcagcaagg tgcagcagat gaggaagagc
    30961 tgattgctgg tgctcgtggt gctgactttg atggtatgcg catccactca gttcgtttgc
    31021 caggtttggt agcccatcag gaagtcatct ttggcaatca aggagaagga ttgaccctcc
    31081 gtcatgactc ctatgatcgc atctccttca tgacaggagt caatttggga attaaagaag
    31141 ttgtcaagcg tcatgagctt gtctatggat tagaacactt attatgagat taatgcaaat
    31201 gccttctgaa tttcagaagg ctttaccagt attagaaaaa attaaagaag caggctttga
    31261 ggcttatttt gttgggggct ctgttcgaga tgcccttctc catagcccta tccacgatgt
    31321 ggatattgcg acgtcttctt atccagaaga gaccaagcag atttttccgc gaacagccga
    31381 tatcggaatc gagcatggaa ccgtcttggt cttagatggg gatgaggagt atgaggtaac
    31441 aacctttcgg acagaggatg tctatgtgga ctatcgcaga cccagtgcgg tttcctttgt
    31501 gcgctcgcta gaagaagacc tcaaacgccg tgatttcaca gtcaacgcct ttgccttgga
    31561 tgagacagga gaaatcgttg acttgttcca tggtttagaa gatttggaaa agcaagtctt
    31621 gcgagcagtt ggagtggcta gtgagcgttt caacgaagat gctttacgga ttatgcgtgg
    31681 tttccgtttt caggctagtc ttggttttgc acttgaacca gaaacattta aagctatgaa
    31741 gaccttgacg ccgcttttgg agaacatttc tgtagagcgt accttcgtcg agtttgataa
    31801 actcttgctg gctccatttt ggagaagggg gttggcttcc atgattgaga gtcaagctta
    31861 tgactatctc cctgatatgg catctagcca ggacaagctc aacagactgt ttgatttgga
    31921 gactgatttt acttttgaat cctctgaaca agcctgggcg gctttactat gggctttgga
    31981 gattgaaaat gcgcagtcat ttttgaaatc ttggaagacc tcacgccagt ttgccaagca
    32041 agttcaggat ttgctgatta ttttggctct gcgtgaaaat ggagaattga gcaagcgaga
    32101 ttgttatcgc tttgacatag atttgctttt acaggctgaa aatcttcgtc aagctcaagg
    32161 aaaagaagtc aacccacaag ccatcacaga aaaataccaa agcttgacta tccatgacaa
    32221 gaaagagatt cagattaatg gcggtatttt gatcaaggaa tatggctatc agccaggccc
    32281 agacttggga gagattttaa cagagattga gtttgccatt gtcgatggag aattggagaa
    32341 taatcgtgaa gccatccatg cttacctgag ggagaaaaaa tgagtgattt tatcgttgaa
    32401 aaactaagta aatctgttgg tgacaagacc gtttttaggg atatttcctt tattatccat
    32461 gacttagaca gaattggttt aatcggtgtc aatgggactg gcaagaccac ccttttggac
    32521 gtcctttctg gtgtttctgg ctttgatggg gatgtcagtc ctttttcagc taaaaatgat
    32581 taccagattg gttacttgac tcaggatcct gattttgatg atagaaagac agttttggat
    32641 acgattctat ctagtgaact caaggaaatc cagctcattc gtgagtatga attgattatg
    32701 ctcgactata gcgaggacaa gcaggcgcgt ttggaacgtg tcatggcaga gatggactct
    32761 ctccaagctt gggaaatcga gagtcaggtc aagaccgttc ttagcaaatt gggcattcaa
    32821 gacttatcta ctcctgttgg ggaattgtca ggtggtctga gaagacgggt acagttggca
    32881 caagtcttac ttggcaacca cgacctcttg cttttggatg agccgaccaa ccatctggat
    32941 attgcgatta ttgagtggct gaccctcttt ttgaaaaatt ctaagaagac cgtccttttt
    33001 atcactcacg atcgttattt cttagacgct ttgtcaacac ggattttcga gttggagcga
    33061 gcaggcttga cagaatatca gggcaattac caggactatg ttcgccttaa ggcagaacag
    33121 gacgagcgcg atgcagctct acttcataaa aaagaacaac tctacaaaca agaactggtc
    33181 tggatgcgca gacaaccgca ggcacgtgcg accaagcaac aagctcgtat caatcgtttc
    33241 catgatctga aaaaggaagt ttcaggcagt agtgctgaga cagacttgac tatgaacttt
    33301 gaaaccagtc ggattgggaa gaaagtcatc gagtttcagg ctgtttcctt tgcctatgaa
    33361 aataagccca ttttgcaaga ttttaatctc ttagttcagg ctaaagaccg tattggaatt
    33421 gttggggaca atggtgttgg aaaatcaacc ctacttaacc tgattgcagg aagtcttgag
    33481 ccgacagcag gacaagttgt gattggggaa actgttcgca tcgcctattt ctctcaacaa
    33541 attgagggtt tggatgaaag caagcgtgtg atcaattacc tgcaggaagt ggcagaggag
    33601 gtcaagacca gtggtggttc tacgacttcc atcgctgagt tgctggagca attcctcttc
    33661 ccacgttcga cgcatgggac cttgattgag aaattgtcag ggggtgagaa aaaacgtctt
    33721 tatctcctca aactgctttt ggaaaaacca aatgttcttc ttttagacga gccaaccaat
    33781 gacctagata ttgcaacttt gacagtctta gagaatttct tgcaaggttt tgcaggtccc
    33841 gttttaacag tcagtcacga ccgctatttc ttggataagg tagcgaccaa gattctcgct
    33901 tttgaggatg gcaagattcg tcctttcttt ggtcattaca ccgactatct tgatgaaaaa
    33961 gcttttgaaa cagatatggc caatcaagtg caaaaggccg aaaaggaaaa agtggtcaag
    34021 gttcgagaag acaagaaacg catgacctac caagaaaagc aggagtgggc aagtattgaa
    34081 ggtgatattg aaaccttgga aaaacgtatc gctgctattg aagaggaaat gcaggctaac
    34141 ggctctgact ttggtaagct ggctactctc caaaaagaat tggatgagaa aaatgaagca
    34201 ctccttgaaa aatacgaacg ctatgagtat ctcagtgaat ttgatagtta atactattcg
    34261 aaaatcaaag tgtaaactat gtcggcttca cattgccgac tcaagtacag cctgtggccg
    34321 gcttcctagt ttgcactttg atttttattg agtataaaag aatagaaaaa tcccatctca
    34381 tgaacggatt tctctattct tttttggttt cttgatgaaa gatattttgc caggtttcgt
    34441 ggcgacgcca gatactggta tggatgatac catctaactc atagcagatg agtttggttt
    34501 tttgactgat ggaagtaatt tgaatgtcct tgatagcaga atttaactct tttttggtct
    34561 tataagcctc tttatccatc tgctctccat cttggcgaat atagacaaaa tcatcagtca
    34621 agagttcttc tagttggttt ccttggtcaa tcaattgttc acgcataagc aattttttat
    34681 agacattgtc taaaatttca tcctcaggga taggtgctgg ttcgatatgg acatcggtat
    34741 caaagacgcc aaaacgattc tccagcatag actcgacctg atccgcgatt tcatggcttt
    34801 caaaaacaga caagtcagga ttcatctcta gtgtaatatc caggtagatg ttgctaccgt
    34861 aggtgcgacc tctttgcgat ttgaccttgc tgattttggg aatttccatg atagcctttt
    34921 ggtagtcctc gagcaggcgg tcgtcaaagc catctgaaag actaaaggaa gactcgatga
    34981 agatatcata ggcagtcttc aagataaaga aagtgatgat gatagcaacc agtttatcca
    35041 caatcggata attgaaacta ctagctagga tggcaatggc ggttccaagt gaggtaacag
    35101 cgtcagaaag attgtcctta gcagctgctt tcagcgcctt ggagttggat ttcttactga
    35161 ggcgagtatt gtagagatag accacaaaca taatcgctgc agaaatgatt cctagagttg
    35221 caccaagagg atcaatgacc gtttcttccc gactgagaat cttttgaatg gtatctctta
    35281 gaacatcgaa accgacatag aacatgatga tagaagtgat caagcttgcc aaatcttcaa
    35341 tcttccaatg accaaaacgg tggtcacggt ctgcaggctg gcgcgccatc cgaatcccga
    35401 ttaagagggc cacatttcca atgatgtccg atacgttatt aaaaccatcg gccaccaaac
    35461 tggatgaatg aaggagatga ccagttgcta atttggctgc agacaagatc aaataggtcg
    35521 aaatactgat aatggctcca cgctcagcta acttgagatt tgagatagat tgcttcattc
    35581 aacttccttt ctagtcacat agcattctac cattatactg gttttcaagg aaaaattcaa
    35641 ataaggcagt ttttactctt tgaaaactcc ccaaaatttg gtataatagt actactcaag
    35701 ggagtagctg gcagaaacct gtgatagtgt cgtcattccg aattttatgc tgaaaagtat
    35761 gctttccggc cctatcttaa acagcgagac ttgttatgat taacaggtct ctttttgttt
    35821 gtcgtgaaaa gatatgacag aaagagagtc tgttttgcac acaatgtcaa ggaggagaca
    35881 catgtcaaaa gaacaaaaac gccaagcgtt ttatactcag agccctgaag aggtcttgca
    35941 ggctgtggat gcgaccgagc aaggtttgtc atcaagtgag gcggaaaagc gccttgtcga
    36001 atttgggcac aatgaactcg aagaaggcga gaaacgatca atcttggtca aattcatcga
    36061 gcaatttaag gatttgatga ttatcatcct agttgcggca gcaatcttgt cagtcgtgac
    36121 ttctggtggg gaagatatcg cagatgccat tatcatccta gctgtggtta tcatcaacgc
    36181 tgcctttggt gtttaccaag aaggaaaagc tgaagaagct attgaagccc tcaaatccat
    36241 gtctagtcca gttgcccgcg ttcttcgtga tggacacatg gcagagattg actctaaaga
    36301 attggtacca ggcgatatcg ttgcccttga agcaggtgac gtggtaccag cggacctacg
    36361 tttgatagaa gccaactctc ttaaaattga agaagcagcc ttgacaggtg aatctgtacc
    36421 agtcgaaaaa gacttgtcag tcgagcttgc gacagatgct ggtattggtg accgtgtcaa
    36481 catggccttc caaaactcaa acgtaaccta tggtcgtggg atgggtgttg ttgtcaatac
    36541 aggtatgtac actgaagttg gtcatattgc tggtatgctt caagatgcgg atgagactga
    36601 tacaccactc aaacaaaact tgaacaacct ttctaaggtc ttgacctatg ctatcttggc
    36661 cattgccctt gttacttttg tagtgggtgt cttcattcaa gggaaaaatc cacttggtga
    36721 gttgttgact tctgttgccc ttgccgttgc agccattcca gaaggacttc ctgctatcgt
    36781 taccatcgtt ctttctcttg gtactcaagt tttggccaaa cgacattcca tcgttcgtaa
    36841 gttgccagca gttgaaacac ttggttcaac tgaaatcatc gcttctgata agactggtac
    36901 gctgactatg aacaagatga cagtcgaaaa agtcttctac gatgcggttc tacatgactc
    36961 agctgatgat attgaactag gtcttgaaat gccactactt cgttcagttg tcttggccaa
    37021 tgatacgaaa atcgatgtgg aaggtaactt gattggtgac ccaaccgaaa cagcctttat
    37081 ccaatatgcc ttggacaagg gctatgatgt caaaggtttc ttagagaaat atcctcgtgt
    37141 agctgaattg ccatttgact ctgaccgtaa gctcatgtca acagttcacc cattgccaga
    37201 tggtcgtttc cttgtagcag tcaagggtgc gccagaccaa ctcttaaaac gttgtcttct
    37261 tcgtgataag gctggggata ttgctccgat tgatgagaag gttacaaatc tcattcatac
    37321 aaacaattct gaaatggctc atcaagcctt gcgtgtcctt gcaggtgctt ataagattat
    37381 cgatagtatt ccagaaaatc tcacttctga agagcttgaa aatgatttaa tttttactgg
    37441 tttgattggg atgattgacc ctgaacgtcc tgaagccgct gaggctgttc gtgtggctaa
    37501 ggaagcggga atccgtccaa ttatgattac aggtgaccac caagacacag cggaagccat
    37561 tgccaaacgt ttgggaatca ttgacgcaaa cgatacagaa ggtcacgttt taactggtgc
    37621 tgaactcaat gaactgtcag atgaagaatt tgaaaaagtc gttggtcaat actctgttta
    37681 tgcccgtgtg tctccagaac acaaggttcg tatcgtcaag gcttggcaaa aacaaggtaa
    37741 agtcgttgcc atgacaggtg acggtgtcaa tgacgcgcca gctctgaaaa cagccgatat
    37801 cggtatcggt atgggaatca ctggtacaga ggtttctaag ggggcttctg atatgattct
    37861 tgcagatgat aactttgcga ctattatcgt cgcagtggaa gaaggacgta aggtcttctc
    37921 aaacattcaa aagactattc agtacctact ttctgctaat actgctgaag tattaaccat
    37981 cttcctatca accttgtttg gttgggatgt cttgcagccg gttcatcttt tgtggatcaa
    38041 cttggtaacg gataccttcc cagctatcgc tcttggtgtt gaacctgcgg agcctggtgt
    38101 catgaatcat aaaccacgtg gacgcaaggc aagcttcttc tcaggtggtg ttttgagttc
    38161 tatcatttat caaggtgtac tccaagcagc tcttgttatg agtgtttatg gccttgcgat
    38221 tgcttaccca gttcatgtgg gtgacaatca tgctattcat gcagatgccc taacgatggc
    38281 ctttgcaacc cttggtttga ttcagctctt ccatgcctac aatgtcaagt ctgtctacca
    38341 atccatcttg acagttggcc cattcaagtc taagaccttt aactggtcca tcttggtatc
    38401 ttttatcctt ctcatggcaa caatcgttgt agaaccgctt gaaggaatct tccacgtaac
    38461 caaactagac ttgtcacaat ggggaattgt tatggctgga agcttctcaa tgattatcat
    38521 cgtcgaaatc gttaagttta ttcaacgcaa actcggtttt gacaagaatg cgatttaagg
    38581 gaggtttcgg atgtcagcat atcaattacc gaccgtatgg caggatgaag ctagtaatca
    38641 aggagctttt acggggctaa acagaccaac agcaggtgcc cgtttcgaac aaaacttgcc
    38701 aaaaggagaa caagcttttc agctttattc actgggaaca ccaaatggtg tgaaggttac
    38761 tatcttattg gaagaattac tagaagctgg ttttaaggaa gcggcttacg acttgtataa
    38821 gattgctatc atggatgggg atcaattcgg ctcagacttt gtgaagctca atccaaattc
    38881 caagattcca gccttattgg accagtcagg tactgaaaac gtaagagtct ttgagtctgc
    38941 tcatattctt ctttaccttg ctgagaaatt tggagccttt ttaccaagta atcctgtgga
    39001 aaaggtagaa gttttgaatt ggctattctg gcaagcaggt gcagcacctt ttctaggtgg
    39061 gggatttgga catttcttca attatgctcc tgaaaaattg gaatatccta ttaaccgttt
    39121 tacgatggaa gtgaaacgcc agttggattt attggataag gaattggctc agaaacctta
    39181 tattgcaggc aatgactata cgattgcaga tattgctatc tggtcttggt atggacagtt
    39241 agttcaagga aatctttacc aaggttctgc aaaattcttg gatgcctcaa gttatcaaaa
    39301 tctagtaaaa tgggcagaaa aaattgccaa tcgtccagct gttaagcgtg gattggaagt
    39361 aacttataca gaaattaaat agcaaaaaag tcatcttcgg atggcttttt gctacagttg
    39421 agggaaaggg tttgcagtta tcggcttttg tgctataatt gctataatat agtaaaaagc
    39481 aagaggagtg tgaggaagtg gaaaagaaaa ttgtgaagga tatcttattt ttatctcaag
    39541 tgtctcagcc ggcaagtcag gaggaccttt atcttgccag agatttgcag gatacactct
    39601 tagcaaatcg tgatacctgt gttggtctag ctgccaatat gattggggtg cagaagcgcg
    39661 tgattatctt taatcttggc ttggttcctg tggtcatgtt taacccagtg cttctgtcct
    39721 ttgaaggatc ctatgaggca gaagaaggct gtttgtcctt ggtaggtgtg agatcaacta
    39781 agcgttatga aaccataagg cttgcctatc gtgacagcaa gtggcaggaa cagaccatta
    39841 ccttgacagg cttcccagct cagatttgcc agcatgagct ggatcacttg gaaggacgaa
    39901 tcatttagga ggaaagcaaa tgaaacgaat agtctttgaa cttattttta tcgcaacgac
    39961 ctggtatatc tttttaccgc cccttaacct gaccagctgg gaatttctct tcttcctctg
    40021 tgggcatttg ttagttgtgg caatattatt tggctttggc aaggggataa accttgtcaa
    40081 aacggttcat gtgcgccacg gtaaggcgga agctgcctta aatcttgagg gtttcaaaat
    40141 caatcggtta gggaaaattc tgttagcttc gattggagga attcttctct tggcagcttt
    40201 ggtttccttg gtaacttcca gcatgtttca ggctaaaaat tatgccaatg tagtcacggt
    40261 tacggaaaaa gactttactg aatttcctaa gagtgacacc agtaaggttc ctatcctaga
    40321 tagaagtact gctgaaaaaa ttggagaccg ctacttgggt tccctaaccg ataaggtgtc
    40381 gcaatacgta gcggcagata cctataccca attgacaatt gatgggaaac cttatcgggt
    40441 cacaccacta gaatatgcag accctatcaa atggtttaac aatcaagcca agggaatcgg
    40501 tgagtatatt aaggtggaca tggtaactgg aaatgcggat ttggtggact tgaagacacc
    40561 aatcaagtat tcagactcgg agtattttaa ccgtgatgtc aaacgtcacc tgcgcttgaa
    40621 gtacccgacc aaaatcttta aaactccatc ttttgaggtg gacgatgagg gcaatccttt
    40681 ctatgtagca acggtttacc aaaagcaatt tggacttgct gttcctcgtc ctgcttcagt
    40741 cattatcttg gatgctacaa atggagaaac caaggaatac agcttatcag atgttccaga
    40801 atgggtggac aggatctatc cagcagagga aaccattgag caaatcaact acaacggcaa
    40861 gtacaaggac ggtttcttga atgccatgat ttccaagaaa aacgtgaccc agactaccaa
    40921 tggctataat tacttgtcta tcggtaatga catctatctc tacacaggtg tgacgtcggc
    40981 taatgcggat gagagtaatc ttggtttcat ccttgaaaat atgcgaacag gagaaatcac
    41041 taagtatagc ttggcttctg cgacagaaga atcagcccgt gaatcagcag aaggtgctgt
    41101 tcaggagaaa tcctacaaag caaccttccc aatcctcatc aacctcaatg acaagcctct
    41161 ctacatcatg ggcttgaagg acaatgctgg cttggtcaaa gagtacgccc tggtagacgc
    41221 agtcgagtac caaaatgtta tcgttgctac tacagtggaa gagatgctca gcaagtatgc
    41281 caataaaaac gaccttgaaa ttgacaatgc aacgacagaa agcatcaatg gagtagtagc
    41341 agacctcaaa tcagctgtta tcaagggaga cactgtctac ttctttaaag ttgatggcaa
    41401 gatatacaag gtcaaggctt cagtatccga tgaccttcct taccttgaaa atggtaaaac
    41461 cttcgaaggt caagtaggaa aagacaatta tctcaagacc tttaagctac ggtaaaaata
    41521 ggtttttttc agaaagtata tgttataata aggtaaatta agcctaatgg aggacaaaga
    41581 aatgggtttt tatttgatgg ttgcttcaat gctactcgga ctgctcgctt tgaagatagg
    41641 tttttctcag ttcaaggaga aaaaagataa attcttatct atcttaacaa gtttagctgg
    41701 cacagccctt gttctcgtgg ctgtctggtt aggatggccc aaataaatag aaaaacctcg
    41761 gtcaaaccga ggtttttcaa atgaatttct tggttgtggc taaaaaatat gctacactat
    41821 caatatgaaa attttaatcc caacagcaaa agaaatgaac acagacttgc caagtatcga
    41881 ggcaattcct ttaaaaccag aaagtcaggc cgtgcttgat gccttggctc tctattctgc
    41941 cagtcaattg gagagtttct acaaggtatc agctgagaaa gcggcggaag aatttcaaaa
    42001 tatccaagct ttgaaaaggc aaactgctca acactatcca gccttgaaac tttttgatgg
    42061 gcttatgtac cgcaacatta agagagataa gctgaccgag gcggaacaag attatcttga
    42121 aaatcatgtt ttcattacct cggctttgta cggtgttgtt ccagtcttgt cacccatggc
    42181 tcctcaccgt ttggattttt tgatgaaatt aaaagtcgct ggtaagactt tgaagagcca
    42241 ttggaaggca gcctatgatg aaactctgaa gaaggaagaa gtgattttct ctctcttgtc
    42301 atcagagttt gagactgtat tttctaagga aatcagagca aagatggtga ccttaaaatt
    42361 catggaggat agaggcggtc agctgaagat tcactcaact atctccaaga aagcgcgcgg
    42421 ggcctttcta acagctttaa tagaaaatca agtacaaact gtgggggaag cacgtcgctt
    42481 gaactttgct ggatttgttt accgagaaga tttgtcacaa ccacagggat tggtttttgt
    42541 aaaggaagta tagaaatcag atattttgta tttggcaata tgtatcatca gctattttag
    42601 tttataaatg agaaaaaagc gaacaagcta gtcttctgag tgtcagaaaa gtagtcttgt
    42661 tcgcttttat tatgttatac tcttcgaaaa tctcttcaag ccacgtcagt gtcgccttac
    42721 cgtatatatg ttactgactt cgtcagttct atctacaacc tcaaagcagt gctttgagta
    42781 gcttgcggct agcttcctag tttgcttttt gatttttatt gagtgttagt tacttgatag
    42841 cttcgaccaa gtcttgagct tgtttttcaa gtgagtttag gactgtttct tcaagaacca
    42901 attttccgtc tgcccaggca gagtcattaa cacgtgcagc agtgaaatca ccaacgcctt
    42961 gtgtacggat aaatggcaag aggtctttgt agatagcgaa aagttgatcg tgccctgcat
    43021 tggctacaga tgatactgtg acaaacttgt cttgaagggc agaaacgcca cgtgtatcag
    43081 acaagtcaag ggcacgagat agccagtcaa gcaagttttt cactgtacca gggatagaga
    43141 agttgtagac tggagagaaa atccagatag catccgcaac gagaactgct tcacgagcag
    43201 cagctacagc tggatgtgtt ggaacttcca aatcttggct gaagagagga agggctgaat
    43261 aatcaaggta gctaacttcc gctttaccag caagtgcttt ctcagcttcg agcgccattt
    43321 ggtggttgaa agaaccttga cgtagtgatc cgacgataaa taatactttt ttagacatga
    43381 tgtgtctcct ttttcttaaa atttcgagaa ctctctcgtt gttataaaat atattacaac
    43441 aaaacaaaag aacttgcaag aaatttgctc agagtttatt tgaaaattgt aaaatttcgg
    43501 aaactctagt catttgtgac tgaaaaagta agaactgttt cactttcaaa atcggttttg
    43561 tttgaaggga gaaatagaat tggttgctca aagcacagct ttgaggttgc agataaagct
    43621 gacgtggttt gaagagattt tcgaagagta ttcttatatt cacttgtaat aaagcttaat
    43681 agatggtaaa atagacgagt gaaaaaaaga caaaacaaag caaaaaataa tctactgtgg
    43741 cagtatggtc tagggatgac gattttgttt gtggttatca gtgcttcctt tctgtatatg
    43801 gtttctctta gcatgaaacc ctatcaaaca gctaaaagtg aaggagaaaa attagctcag
    43861 cagtatgcag gattagagca ggccgatcag gttgatttat acaatggctt ggaatcttat
    43921 tacagcgttc ttggtcgtaa taaacagcaa gaagcacttg ctgttctgat tggaaaagat
    43981 gatcataaga tttacgttta tcagctaaat cagggtgttt cacaagaaaa agcagaaacg
    44041 gtttctaagg aaaagggagc tggcgaaatt gacaagatta tctttggtcg ttatcaagat
    44101 aagccaatct gggaagtcaa gtcaggatct gatttttatc tagtagattt tgaaacagga
    44161 gcattggtca acaaggaggg cctatgaaat tatccaaccg tgttttagaa atggaagaaa
    44221 gtgtgacttt ggctgctgga gcccgtgcca aagcactgaa ggctgagggc agagatattt
    44281 tgtctctaac cttgggtgag ccagatttca ctactcccaa aaatatccaa gatgccgcca
    44341 ttgcatcgat tcgagatggg cgtgcttctt tttatacagt aacctcaggt ctgccagagc
    44401 ttaaggcggc ggtcaatagc tactttgagc gcttttacgg ctattctgta gcgtcaaatc
    44461 aagtgacagt cgctgcggga gccaaatatt ctctctatac cttctttatg gctgtggtca
    44521 atccaggtga tgaagtcatc atcccaaccc catactgggt tagctatgga gatcaggtca
    44581 agatggcaga aggcgttccc gtctttgttt ctactaagga agacaatcac tttaaggtga
    44641 ccgtagatca gttagaagca gctcgcactg acaagaccaa ggttttggtg ctgaattcgc
    44701 catctaatcc cacaggtatg atttacaccc gtgaggaact cttggcaatt ggaaactggg
    44761 ctgtagaaaa tgatattctc atcctagcag atgatatcta tggccgcttg gtttataatg
    44821 gtcatgagtt cacaccgatt tctagcctat cggaagcgat tcgcaagcaa acagtggtca
    44881 tcaatggtgt gtctaaaacc tatgccatga ctggttggcg gattggttat gccgttggag
    44941 aagcagacat tatcgctgcc atgtccaaga ttgcaggtca aacaacttcg aatccgtcag
    45001 cagtagccca atatgcagca gttgaggctc tatcaggtga gcaagatact gtagaaagca
    45061 tgcgtcaggc ctttgaggaa cgtcttaata ctatctatcc cctccttgca gaggtgccag
    45121 gatttgaagt ggtcaaaccg caaggggcct tctatctctt cccaaatgtc aaaaaggcca
    45181 tggagatgaa aggctacacg gatgtgacag actttacaac tgctatctta gaagaagccg
    45241 aagtggcctt ggtaacagga gctggatttg gagcaccaga aaatgtccgc ctcagctatg
    45301 cgacagacct agacacgctt aaagaagcag tcgaacgctt gaaagcattt atgggtagtg
    45361 agaatgattg atcattttga gattaaggta aaggatttac aaatttcaga aggattttat
    45421 aggagttttc tcgctccttt ggactataaa atggctttta agactagttc tctaattagt
    45481 tttctatccc cgaacagccc tcatcctggt ggtgattttt ggctgactca gggaacacaa
    45541 gatcctgttc attttgcttt tttagcagaa aataaggaag aagttcaggc ttgttatgag
    45601 gctggcttag aggcaggtgg gcgagacaat ggggttcctg gttatcgaag tgagcatccg
    45661 atttattatg ctgcttttat gattgacctg gatgggaaca atatagaggt ggtttgccat
    45721 aaagaataaa aaagaaaagg aaaaataatg acaaaacgtg taacgattat tgatgtaaaa
    45781 gactatattg gtcaagaagt gacgattggc gcttgggttg ccaacaaatc aggaaaaggg
    45841 aaaatcgctt tcttacaatt gcgtgatgga acagccttct ttcaaggtgt ggcttttaaa
    45901 ccaaactttg tcgaaaaatt tggtgaagaa gtgggacttg agaagtttga tgttatcaaa
    45961 cgcttgagcc aagaaacgtc tgtttatgtg acaggtattg tcaaagagga cgaacgttct
    46021 aaatttggct atgagttgga catcacagac atcgaagtga tcggtgaatc tcaagactac
    46081 ccaatcacac caaaagaaca cggaacagac tttttgatgg ataaccgtca cttgtggcta
    46141 cgctctcgta agcaagtagc tgtgttgcaa atccgtaacg ctattatcta tgcaacttat
    46201 gagttctttg acaagaacgg ttttatgaag tttgacagcc caattctttc aggaaatgcg
    46261 gcagaagatt ctacagaact ctttgaaact gactacttcg gaacgccagc ctacttgagc
    46321 caatcaggtc aactttacct agaagcaggg gctatggctc ttggtcgtgt ctttgacttt
    46381 ggtccagttt tccgtgctga aaaatcaaaa acacgccgtc acttgactga gttctggatg
    46441 atggatgctg agtactcata cttgacacat gatgagtcgc ttgacttgca agaagcttat
    46501 gtgaaagctc ttctacaagg tgttcttgac cgcgcgcctc aagccttgga aaccttggaa
    46561 cgtgatacag aactcttgaa acgctacatt gcagagccat tcaaacgtat cacttacgat
    46621 caagccattg acctcttgca agagcatgaa aatgatgaag atgctgacta cgagcatctt
    46681 gagcatggtg atgactttgg gtcaccacac gaaacttgga tttcaaacca ctttggtgtg
    46741 ccaacatttg tcatgaacta tccagcagcc atcaaggctt tctacatgaa accagttcct
    46801 ggaaatccag agcgcgtgct ttgtgcagac ttgcttgctc cagaaggcta tggagaaatt
    46861 atcggtgggt ctatgcgtga ggaagattac gatgcccttg tcgctaagat ggatgaactt
    46921 ggcatggatc gtacagaata tgaattctac cttgaccttc gtaaatacgg tacagttcca
    46981 cacggaggat ttggtatcgg tatcgaacgt atggtaacct tcgcagcagg aacaaaacat
    47041 atccgtgaag ctattccatt cccacgtatg ttgcaccgta tcaaaccata agaacaggaa
    47101 aacgcccatg tggcgttttt tcgaattcta gtgtttttac ttgcgtaaaa aaattttttc
    47161 tagtataata gtttattgtg agcgaacctc acttacccct tgcaaagtct tggggtcatt
    47221 agaccaaaag gaggaacata tcaatggcta aatacgaaat tctttatatc attcgtccaa
    47281 acattgaaga agaagctaaa aacgctttgg tagcacgttt tgactctatt ttgactgaca
    47341 acggtgcaac tgttgttgaa tcaaaaactt gggaaaaacg tcgtcttgca tacgaaatcc
    47401 aagatttccg tgaaggactt taccacatcg ttaacgttga agcaaatgac gatgcagctc
    47461 ttaaagagtt tgaccgtctt tcaaaaatca acgctgacat tcttcgtcac atgatcgtca
    47521 aaattgacgc gtaagaaggt aaactatgat taacaatgtt gtacttgtag ggcgtatgac
    47581 acgtgacgct gagttgcgtt ataccccatc aaatgtagca gttgcgactt ttactcttgc
    47641 agtaaaccgt acatttaaga gtcaaaatgg tgaacgtgag gctgatttta tcaatgtcgt
    47701 tatgtggcgc caacaggctg aaaatcttgc taactgggct aaaaaaggct cacttatcgg
    47761 ggtgacaggt cgtatccaga ctcgtagtta cgataaccag caaggacaac gtgtctacgt
    47821 gacagaggtc gtggctgaga atttccaaat gttggaaagc cgtagtgtgc gtgagggtca
    47881 cacaggtgga gcttactctg caccaactgc aaactattca gcacctacaa attcagtacc
    47941 agacttttca cgtaatgaaa atccatttgg agcaacaaac ccattggata tttcagatga
    48001 tgatttacca ttctaatgga caactaaact tataaaggag aaaaaacatg gctcaacaac
    48061 gtcgtggcgg attcaaacgc cgtaaaaaag ttgattacat cgcagcaaac aaaattgaat
    48121 atgttgatta caaagatact gagcttctta gccgtttcgt ttcagaacgt gggaaaatcc
    48181 ttcctcgtcg tgtaacagga acttcagcta aaaaccaacg taaagtaaca acagctatca
    48241 aacgcgctcg cgtaatggct ttgatgcctt tcgtaaacga agattaaaga aaagacccgg
    48301 ttgggtctct ttttcatgag cttgaaaaca agaaagcgaa ttaagacccc aacgggtctt
    48361 ttttcacaag ctttgaaatg agagagcgag ttaggctcag tggcttcctt tttacgagtt
    48421 tgaaaacaag agaacacatt aaaagcacga tggagctttt tcataaatat gtctcttttc
    48481 tcatccaaga aattaaaata aaaatgataa actttttgaa aataggggat ttgcctactt
    48541 tatgatataa tggaaagagt tagattgaaa gaggtagtga gatggacgcg aaattaagat
    48601 acaaggcaaa gaagatcaag attgtctttt ttgatattga tgatacattg cggaattcaa
    48661 agacagggtt tattccaact acaattccca ctgtttttaa acagttgcgt gaaaaaggaa
    48721 ttttaacagg aatcgcctct ggacgtggca tttttggtgt tgttccagag attcgtgatc
    48781 tcaagcctga cttttttgta actttgaatg gggcttatat cgaagataaa aaaggtcagg
    48841 tcatttatca gcatcagatt gaaaagtcag atgttgagga gtatatctct tgggctaagc
    48901 aagaaggaat tgagtatggc ttggttggga gtcatgatgc caagttgtcg actcgcaccg
    48961 atatgatgag tgaagctatc aatccaattt atcccgactt agatgtagat cccgatttcc
    49021 atgaaaaaga agatatctat cagatgtgga cttttgaaga taagggagat gacttgcact
    49081 tgcctgacag tctctcagac aaacttcgca tggttcgttg gcatcaacat tcgtctgata
    49141 ttgtgccgat ttcaggctcc aaagcgacgg gggtggaaaa ggttgtggaa caccttggct
    49201 tgaaaccaga gaaggtcatg gtttttggag atggtctcaa tgacttggaa ctctttgatt
    49261 atgctggaat cagcgttgcc atgggaattt ctcatgataa aatcaaagaa aaagcagatt
    49321 atattacaaa aacattagaa gaagatggca tttttgatgc cttagaagta tttggtatgg
    49381 tagaaaaaga attgcatttt ccacaagtag acattgaaac agtagaaggt cttcttgcga
    49441 ctattaagac caatcacgga gacttacgta tcaagctttt ccctgaacat gctcctaaaa
    49501 cagtggctaa ctttgtatct ctttcaaaag atggctacta tgatggtgtc attttccacc
    49561 gtattatcaa ggactttatg atccaaggtg gagacccaac tggaactggt atgggtggcg
    49621 agtcaatcta cggcgaatct tttgaggatg aattctcaga agagctttac aatatccgtg
    49681 gtgctctttc catggcaaat gctggtccaa ataccaacgg cagccagttc tttatcgtgc
    49741 aaaaccaaca cctaccttat tcgaagaaag aaattactcg tggtggttgg ccagaaccga
    49801 ttgcagaaat ctatgccaat caaggtggga cacctcacct agaccgccgt cacacggttt
    49861 ttggtcagtt agctgatgaa gcatcttacg ctgtcttgga tgccattgct gctgttgaga
    49921 caggagctat ggacaagcca gttgaagatg ttgtaattga aactattgaa atcgaggact
    49981 aagatgaaaa tcggtgataa gctaaagggg cgtattacag ggattcagcc ctacggtgcc
    50041 tttgttgagt tagagacggg tgatacgggg ctgattcaca tttcagaaat tcggacagga
    50101 tttattgaaa atattcatga aaccttgaaa gtcgatgaag aggttcaagt tcaggtagtg
    50161 gatttagatg aatttacggg gaaagccagt ctttctatcc gcactttgga ggaagaaaag
    50221 taccagtttc ctagacggag acgcttttca agtgaccgct ttaactacgg ttttgctccc
    50281 tttcgacgaa tgctaccaat ctggactgga gaagctctgc accatttaaa aaagaagaag
    50341 tagtttagag ttttatattt tttgtaatgt taatataaat ttattataat aggaccatgg
    50401 aagaagaaca attattaaaa tcaggagagc gcattaacca gctcttttcg acagatatca
    50461 aaatcattca aaatagagag gtttttagct attcggtgga tagtgttctc ttatcacgat
    50521 ttccacgttt tcctaagaag gggttgattg tggatttctg tgctgggaat ggagcagtgg
    50581 ggctttttgc tagcactcgt actcaagcac agatattgtc tgttgagatt caggagcgtt
    50641 tggcggatat ggctgaacgc tctgtccgtt tgaatggatt agaggagcaa atgcaggtca
    50701 tctgcgatga tttgaaaaat atgcctgctc acattcaggg gagtaaggta gatatgattt
    50761 tgtgtaatcc accctatttc aaggtggatc cttattctaa tctgaacgag agtgaacatt
    50821 atctcttggc tcgacacgaa atcacgacta atttggaaga aatctgtcgt agtgcccaga
    50881 gtattctcaa gtctaatggg cgtttggcca tggttcatcg tcctgatcga cttctggata
    50941 ttttggatac gttaaaacga cataatctag cccctaagcg cctgcagttt gtttatccaa
    51001 aaagagaaaa ggaagccaat atgcttttga ttgaggcgat caaggatggc tcaacaagtg
    51061 gctttaaggt cttacctcct ttgattgtcc acaatgatga tggctcttat acgcccgaaa
    51121 tcgaagagat ttattatgga tcataaggct tatatgtacg ttctggagtg tcgtgatgga
    51181 tcctactata taggctatac gactgatgtg agaagacgcc tcgctatcca caatagtggg
    51241 aagggagcca aatatacacg agcacgcttg ccagtcaaac ttatctatgc tcaaggtttt
    51301 gccagtaagg aagaagccat gtcggctgaa gctcttctca agcgtaagaa gaggccacag
    51361 aaggaagaat ttttatctga aaatcaagat agaaatttac tcagttattt tgaagaatcg
    51421 tggggagtcc tttgactcct cttacctttg tcaaaaagcg aaaaaaatgc tacaataaag
    51481 aaaataaaca aaatgaggta ttatcatgtc caagattcta gtatttggtc accaaaatcc
    51541 agactcagat gccatcgggt catctgtagc ttttgcctac cttgcaaaag aagcttacgg
    51601 tttggatacg gaagctgttg cccttggaac tccaaatgaa gaaacagcct ttgtcttgaa
    51661 ctattttggt gtggaagcac cacgtgttat cacttctgcc aaagcagagg gggcagagca
    51721 agttatcttg actgaccaca atgaattcca acaatctgta tcagatatcg ctgaagtaga
    51781 agtttacggt gttgtagacc accaccgtgt ggctaacttt gaaactgcaa gcccacttta
    51841 catgcgtttg gagccagttg gatcagcgtc ttcaatcgtt taccgtatgt tcaaagaaca
    51901 tggtgtagct gtgcctaaag agattgcagg tttgatgctt tcaggtttga tttcagatac
    51961 ccttcttttg aaatcaccaa caacacaccc aacagataaa atcattgctc ctgaattggc
    52021 tgaattggct ggtgtgaact tggaagaata tggtttggca atgttgaaag ctggtaccaa
    52081 cttggctagc aaatctgctg aagaattgat tgatatcgat gctaagactt ttgaactcaa
    52141 cggaaataat gtccgtgttg cccaagtgaa cacagttgac atcgctgaag ttttggaacg
    52201 ccaagcagaa attgaagctg caatgcaagc tgccaacgaa tcaaacggct actctgactt
    52261 tgtcttgatg attacagata tcgtcaactc aaactcagaa atcttggctc ttggtgccaa
    52321 tatggacaag gtcgaagcgg ctttcaactt caaacttgaa aacaatcatg ccttccttgc
    52381 tggtgccgtt tcacgtaaga aacaagtggt acctcaattg actgaaagct ttaatgcgta
    52441 agattttggg tgtcagctca aaatcggaaa gtctagtttg ccttatatcg aaaggagttt
    52501 cggctccttt tttctaggag tgaagtatgt tagaaaatgg cgatttgatt tttgtgagag
    52561 atgggtcaga catgggacag gccatccaga cttccacagg taactatagc catgttgcca
    52621 tttatttgga tgggatgatt tatcatgcta gtggacaggc tggtgttgtc tgtcaagaac
    52681 cggcagactt ctttgagtcc aatcatttgt acgacctcta tgtttaccca gaaatggata
    52741 tccagtcggt gaaggaaaga gcttgcagac atcttggagc accctacaat gcttctttct
    52801 atccagatgc agctggtttc tactgctccc agtatatagc agaaatccta cctatttttg
    52861 aaactattcc tatgaaattt ggagatgggg aacaggagat tagtgatttt tggagggagt
    52921 attacataga actaggtctg cctgttcctc tgaaccaagc tgggaccaat cctagtcagt
    52981 tggcagcatc gcctctgtta caatgtaaag aaaggaatct tcatgattca gatttttaat
    53041 ccatctcgtt tgacgagaca gccatttttg gagaattgat ccgctatctg gaccagtatg
    53101 aggatgtgat tctacgggaa attaaggctc aatttccaga tgttgcagtt gataaactca
    53161 tggaagagta tataaaggca agcttgattc tacgtgaaaa taagcgctat tacctcaatt
    53221 ttcctacgct tgaatcactt gatagtcttg aactggatca agagattttt gtcagagaag
    53281 ctagtccggt ctatcaagcc ttgttggagc agagttttga gacggaattg cgcaatcaaa
    53341 tcaatgcagc tattttagtt gaaagacgga ctttgcgcgc attaaaatga ccctgtccaa
    53401 ttatttttac aaggtcaaac agcagtatcc tttgacagaa aaacagcagg agctctatga
    53461 cattttagga gatgttaatc ctgagtatgc actcaagtat atgacggctt ttttgttgaa
    53521 atttctcaaa aaagaccagc ttatgcagaa atgccgtgat atctttgtgg acagtttggt
    53581 tgtcttaggc tatattgtgc aaaatgaaga tagaaagtat gagttggcta tcgattttga
    53641 taaggagaga ttaactttct acttagcgtg atttcttgtt tctgagcaca ttgtttgact
    53701 ttccttagta ttcggtataa actatatgta accggtaaca catatcggaa taaactaaag
    53761 gagacaatca tatgtcactt gaaaacaaat tggaacaagc aacaggcgct gtcaaagaag
    53821 gttttggtaa agttactgga gacagcaaga cagaacttga aggagctgtt gaaaaaacag
    53881 ttgctaaggc aaaagacgtt gtagaagacg caaaaggtgc tgtagaaggt gccgttgaag
    53941 gtttgaaaaa cgtttttact aaataatagg aaaaaatcaa gggtttcatt ttcccttgat
    54001 tttttctatt cttataaata attttctgcg acggctgtat ctcctgggta ggattctttc
    54061 ttgccctgga tgatttggta acaatcggct cccttacccg caataataac tgcatctaat
    54121 tcgtgatttg tgatagccat tgccgccttg atggcttctt ggcgatccgc aatcttttca
    54181 acaggatgat tgatgtagct actaatttca tctgcaatgg ccattgggtc ttcatagtta
    54241 gggtcatcag cagtcagaaa gacttgaatc tcagggtgtt gattgaggag gaggccaaag
    54301 tccttacgac gactttctcc cttgtttcct gttgatccca gaaccagagc aatctttccg
    54361 gtttgatgag tttcaaccac attgatgagt tttttcagac tatccccatt gtgggcatag
    54421 tcgatgaaga ccttggctcc atttttctga gtgaggactt ccatacgacc aggaacgcgg
    54481 gttgcagcga tgcctttttt gatgtcctca agacttgctc cgagacggag acaagcaagt
    54541 ccagcagcaa ctgcattttc ttggttgaag ttgccaatga gttggatatc ataatctcca
    54601 gcgagtttac ccgtagctga aaagctaaag gctttggaat tctcgatttg gttatcaaat
    54661 tggctaccat agaaatcatg gtcttgatct tcaacctgtt ctttcaagac tgagaagtgg
    54721 tccatgtcac tgttaatgat gactgctcgg ctattttcca tcaagagacg cttgtggtag
    54781 aaatagtctt caaagctaga gtgttcaatc gggccgatat ggtcaggagt gatgttaaga
    54841 aagactccta catcaaaggt cagtccatag actcgatgga ctagataggc ttgactggag
    54901 acttccatga tgaggtgggt acggtcattt tgcacagcct gattcatcat gtcaaagagg
    54961 tcaatactct caggggttgt caacgctgac ttaaaaaaag tctcgccatc aagagttgtg
    55021 ttcatggtcg acaacatagc aggtctatgc ccttgagata agatgttata ggcgaaatag
    55081 gttgctgttg tcttaccctt agtaccagta aaggcaagga gtttgagttt ctcttgtgga
    55141 ttaccataga actccatggc aatcaaactc atggctttct ttatatcgtt cacaatgatg
    55201 acagggatat cgacttcgta gtccttttca gctacatacc aagctaaacc ttgtgttata
    55261 gcagaaagaa ggtattcttt tttaaaggca gcgccttttg caaaaaaaag agtgtcttct
    55321 gttacttttc ggctgtcgta gctgatgcta tcaaaaataa ctttgctgta gttgtagtgg
    55381 taatgacctt ggtcaataat ttcgcgaaaa aggccatctt tctttaaaat atctaatacg
    55441 gtttcaatct taatcatact ttctattgta aaccgaaagt cgtaaattta caagtaacaa
    55501 ggaaaagttt ataatggaag ataaggagtt tttcctagtt atcaaaattg aatgaggaat
    55561 ctatgtcgca cgaaaacaat caccagcagg cccagatgtt acgggggact gcttggctaa
    55621 cggctagtaa ctttatcagt cgcctactcg gggctgttta cattatccct tggtacatct
    55681 ggatgggggc ttatgcagct aaggcaaatg gtctctttac catgggttac aatatctatg
    55741 cttggttctt gttggtttca acagcgggga ttccagttgc ggtggccaag caagttgcca
    55801 agtataatac catgcgagaa gaagagcata gctttgccct gattcggagc ttcttaggct
    55861 ttatgacagg actaggtctg gtttttgctt tagtcttgta tgtctttgct ccttggctag
    55921 cagacttgtc tggcgtgggc aaagacttga tcccaatcat gcaaagcttg gcttggggag
    55981 tcttgatttt cccgtctatg agtgttatcc gaggattttt ccaagggatg aataacctca
    56041 aaccctatgc tatgagccaa attgctgagc aggtcattcg tgttatctgg atgctcctag
    56101 caacctttat cattatgaag ctcggttcag gagattatct agcagccgtt acccaatcaa
    56161 cctttgctgc ctttgtcggt atggtagcca gttttgcagt cttgatttat ttccttgccc
    56221 aagaaggttc actcaaaaga gtctttgaaa caggagataa gattaacagt aagcgtctct
    56281 tggttgatac cattaaggaa gccattcctt ttatcctgac agggtctgcc atccagctct
    56341 tccagatttt ggatcagctg acctttatca atagtatgag ctggtttacc aactacagca
    56401 atgaggactt ggttgtcatg ttttcttatt tctcagccaa tcctaataaa atcacgatga
    56461 ttttgatttc tgtaggggtt tcgattggga gtgttggttt gccacttttg acggaaaact
    56521 atgtcaaggg ggacttgaaa gcagcttctc gtctcgttca ggacagtctc accctactct
    56581 ttatgttctt gctaccagca acggttggag tggttatggt aggagaacct ctttatacgg
    56641 tcttctatgg taagccagat agtttggctc tgggcttatt tgtctttgca gttttgcagt
    56701 ctattatttt aggcttgtac atggtcttgt ctccaatgct tcaggccatg ttccgcaacc
    56761 gcaaggccgt tctctatttt atctatggtt ctattgccaa gctagtcttg caactaccta
    56821 ccatcgccct cttccacagt tatggtcctt tgatttcaac aaccattgct ctcatcattc
    56881 ctaacgtctt gatgtatcgg gatatttgta aagtaactgg tgtcaagcgc aaggtgattt
    56941 tgaagcgaac cattttaatc agtttgctga ccctagtcat gtttctgtta ataggaacca
    57001 tccagtggct gttaggattt ttcttccaac caagtggacg tttgtggagc ttcttttatg
    57061 tagctcttgt cggtgccatg gggggtggac tttatatggt tatgagtctg cgtacctatt
    57121 tattagataa ggtaatagga aaagcccaag cagatcgcct gcgagcaaaa tttaagcttt
    57181 cgtaaggtag catttataaa taaaaggaat aagttgaaca atcttttagg agaacttgaa
    57241 acttattcct tttttagcgt caaaatagct aaaattatct ttcaaaaata gaaaatgagg
    57301 tttttttgat tttttggaaa aaacaattga ctaaacaaac ccagagttgt ataataaaac
    57361 aaatatttaa agcaggaggt tctggaaatg aaaaagtcta agagcaaata tctaaccttg
    57421 gcaggtcttg tcctgggtac aggagtttta ttgagcgcgt gtggaaattc tagcactgca
    57481 tcaaaaacct ataactatgt ttattcaagt gatccatcta gtttgaacta tctagcagaa
    57541 aaccgcgcag caacatccga tattgttgca aatttggtag acgggttatt agaaaatgac
    57601 caatatggga atattattcc atcattagca gaggattgga ctgtttctca ggacggtttg
    57661 acctatacct acaaacttcg taaggatgcc aagtggttta cttctgaggg agaagaatat
    57721 gcgcctgtaa ctgcccagga ttttgtgaca ggtttgcaat atgcagctga taaaaaatca
    57781 gaagctttgt atctagtgca ggactctgtt gctggtttgg atgactatat cactggtaaa
    57841 acaagcgact tttcaactgt cggtgtcaag gcacttgatg accaaacggt tcaatatact
    57901 ttggttaaac cagaacttta ctggaattca aaaacacttg caacgatact ttttcctgtt
    57961 aatgcagatt tcctgaaatc aaaaggggat gattttggga aggcggatcc atctagtatt
    58021 ttgtacaatg gacctttctt gatgaaagca cttgtctcaa aatctgctat tgaatataag
    58081 aaaaacccta attactggga tgctaagaat gtctttgtag acgatgtgaa attgacctac
    58141 tatgatggta gcgaccaaga atcactggaa cgtaatttta cagctggtgc ttatactacg
    58201 gctcgtcttt ttcctaacag ctccagctat gaagggatta aagaaaaata caaaaacaat
    58261 atcatatata gtatgcaaaa ttcaacttca tatttcttta attttaacct agataggaag
    58321 tcttacaatt atacttctaa aacaagtgac attgaaaaga aatcgactca ggaagcagtt
    58381 ctcaataaaa acttccgtca ggctatcaat tttgcttttg acagaacatc ttatggggct
    58441 cagtctgaag ggaaagaagg tgcaacaaag attttgcgta acctagtggt tcctccaaac
    58501 tttgtcagta tcaagggaaa agactttggt gaagttgtag tctctaagat ggtcaactat
    58561 ggtaaggaat ggcaaggtat caactttgcg gatggtcaag acccttacta caatcctgag
    58621 aaagccaagg ctaagtttgc ggaagctaag aaagaactcg aagcaaaggg tgttcaattc
    58681 ccaatccact tggataagac tgtggaagta acagataaag taggcataca aggagttagt
    58741 tctatcaaac aatcaattga atctgtttta ggttctgata atgtagtgat tgacattcag
    58801 caattaacat cagatgagtt tgacagttca ggctactttg ctcaaacagc tgctcagaaa
    58861 gactatgatt tatatcatgg cggttgggga cctgattatc aagacccgtc aacctatctc
    58921 gatattttta atactaatag tggaggagtt ctacaaaatc ttggactaga gcctggagaa
    58981 gccaatgaca aggctaagga agttggactg gatgtctata ctcaaatgtt ggaagaagct
    59041 aataaagagc aagatccagc caaacgttat gagaaatatg ctgatattca agcttggttg
    59101 attgatagtt ctttagttct tccaagtgtt tcgcgtgggg gaacaccatc attgagaaga
    59161 accgtaccat ttgctgctgc ctatggttta accggtacaa aagggattga atcatataaa
    59221 tacctcaaag tacaagataa gattgtcaca acagacgaat atgcaaaagc tagagaaaaa
    59281 tggttgaaag aaaaagaaga atccaataaa aaagcccaag aagaattggc aaaacatgtc
    59341 aaataaaatc taattgtaga taagtttgtg taagaagtct ttgtgcttaa aaacgcataa
    59401 tatcaggtat ataaaatcct agatgttatg cgttttgtta taggaagatt tattagattt
    59461 tatcctcaaa ttgagttatt cccgacttta tccgttgaaa tattataagg agtatttatg
    59521 aaattaaaat tattaagagt agatactaag gtgattatgg ggagtttctt acttgttctg
    59581 tctagtctac ttgctttgtt gcttcccctt atcttaaagg gtttaataga tgggagttct
    59641 attgaaaata taggttccaa agtatttcaa tcgtttttga tttttattgg tcaagccttg
    59701 ttttcttcta ttggttacta tctgtttagt caatcgggtg aaaaaaagat agcaaaaatc
    59761 aggaaaaaag tgatagaggg gttgatttat gcagagaaat ccttctttga taagagccaa
    59821 agtggggagt tgacttctgc cattgtcaat gacacgagtg tcattcgtga gtttttaatt
    59881 acgactttcc caaatattat tctgagttta gttatggtac ttggttccat tgtagtctta
    59941 tttagtcttg attggaatct ttctctactt ttattcatca ctcttccttg tatgatgttt
    60001 attatcttgc tcctttccaa tatcagtgaa aagtatagtc gtcgtttaca ggaggaaatc
    60061 ggatttttaa cagggtaatt gactgaaaag attcaagaac atgaattaat taagactaac
    60121 caagcagaga aaagtgtaca ggatgttttg gataattgta ttgaaagggt acaaaacaat
    60181 tcactgaaat cagatagggt tacttctttt gagaccccgt ttgctctctt atttatcttt
    60241 gcgactatag ctgtgatgct aacctatggg ggttatcggg tcagcgcagg atatatatct
    60301 gtgggaacct tggtttcgtt tttgatttac ctctttcaat tacttaatcc tattagtaat
    60361 atagctaatt ttgtaactgt ttattctagg agcaagggat cttcagttgc actggagaac
    60421 ttgcttgcag ttcctaaaga aaaatttgag tgaggaaaat cggtatcagg acaagggttg
    60481 aattttaacc atgtctattt tggttatgat gaaaatcgac ctgtcttaaa ggatattact
    60541 tgttcaattt tcaaggggca aaaaattgct tttgttggac catctggatc aggaaaatca
    60601 acgattgtgc gtttgttaga gcggttttat aaaccgcttt caggagatat tctaatggag
    60661 caatcaagta tatatgattt taacttaaaa gaatggagaa gtaaaatcgc ttgggtttca
    60721 caaaataatg cagtcttatc tggcagtatt cgtgacaatc tttgtctcgg tttgaatcgc
    60781 ttagtaactg atgatgaatt gatgaaagtg ctagacttag tatcactagg tgatgagatt
    60841 cgctccatga aagagggact agatactgaa gttggtgaac gcggacgact cttgtcaggg
    60901 gggcaaagcc aaagacttca aatagctaga gcctacttaa aagatgctga aattcttata
    60961 tttgatgaag ccactgctaa tcttgatgcg gattctgagt atgcgattat cagtagcctc
    61021 tattctgtat taaaggagaa gacggttgtg attatagcgc atcgtttgtc aacggtaaaa
    61081 gatgtggatt gtattttctt cttagaggag gggaaaatca ctggctcagg aactcataag
    61141 gaactactgg aaaatcatga gcgttatgct cgttttgtgc aggagcaaat gatagagtga
    61201 aggtgtcttt tgagattcac cattttatag tctattaaag ggagcaggaa aaactccctt
    61261 tttatatagt ttgaaactat aactagctct tgaaaagaag aaaatgagtt gatgaaaata
    61321 agtggtacaa tagttactat agatttggag gtattgtatg agcaaggaat tacacattaa
    61381 cacaattttg gcccaggcgg gtattaagtc agatgaagcg acaggtgcat tggtgacacc
    61441 gcttcatttt tcaacgacct atcagcatcc agagtttggt cgatctactg ggtttgacta
    61501 tacgcgcact aaaaatccaa ctcgtagtaa ggctgaggaa gtcttggcgg ctattgagtc
    61561 agcagactat gccctagcga ctagctcagg gatgtcagct attgtactgg cctttagcgt
    61621 ctttccagta ggaagtaagg tcttggcagt gcgtgatctt tacggtggtt cttttcgctg
    61681 gtttaaccaa gtggagcagg aaggtcgttt ccattttacc tatgccaata cagaagaaga
    61741 gttgattgcc gagttagaaa aggatgtgga tgttctctat atcgaaaccc caaccaatcc
    61801 cttgatgttg gaatttgata tcgaaaaact agcaaaattg gctcatgcta agggtgccaa
    61861 agtggtggtg gacaatacct tctatagccc tatctaccaa cgtccgattg aagatggagc
    61921 agatatcgtt ctccattcag caaccaagta tctagcaggc cacaatgatg tcttggctgg
    61981 agtggttgtg accaatagtt tagaactata cgagaagctt ttttacaatc tcaatacaac
    62041 aggggcagtc ttgtctccat ttgacagcta ccagttgctt cgtggtctca agaccttatc
    62101 tcttcgtatg gagcgctcaa cagctaacgc ccaagaagtg gttgcctttt tgaaggattc
    62161 tccagcagtt aaggaagttc tctacactgg tcgtggaggc atgatttcct ttaaagtagc
    62221 cgatgaaaca cgcattcctc atattttgaa cagtctcaag gtcttctctt ttgcggaaag
    62281 tttgggcgga gtggaaagtc ttattactta tccaacgact caaactcatg ctgatattcc
    62341 agcagaagta cgccattctt atggtttgac agatgacctc ttgcgtttgt ctattgggat
    62401 tgaggatgct agagatttga ttgcagattt gcgccaagcc ttagaaggat aagacaaaga
    62461 tgggaaaata tgattttaca agcctgccca accgtttagg gcaccatacc tataaatgga
    62521 aagaaacaga aacggatagt gaagttctac cagcttggat agcggatatg gactttgtgg
    62581 tcttgcctga aatccgccaa gccgtgcaaa cttacgcaga ccaactggtt tatggttata
    62641 cctatgccag tgaagactta attaaggaaa ttcaaaagtg ggaagctaca caatacggtt
    62701 acaactttga caaagaggct cttgtcttta tcgagggtgt ggtaccagcc atctcaacag
    62761 ctattcaaac ctttacaaaa gaaggcgagg cggttttaat taacacgcct gtctacccac
    62821 cctttgctcg cagtgtcaag ttgaataatc gtagattgat tactaattcc ttagtggaaa
    62881 aggatggtct gtttgagatt gactttgacc aacttgaaaa ggatttggtg gaagaggagg
    62941 ttaaactcta tattctttgc aaccctcaca atcctggtgg acgtgtttgg gaaaaagaag
    63001 tgttggagaa gattggccaa ctttgccaaa aacacggtgt tttgttagtt tcggatgaga
    63061 ttcaccaaga tttgaccctc tttggtcaca aacaccagtc tttcaatacc atcaatcctg
    63121 ccttcaaaaa ttttgctatc gtcttgagca gtgccactaa aacatttaat attgctggaa
    63181 caaaaaattc ctatgcagtc attgaaaatc ctaagttgag actggctttc cagaaacgcc
    63241 tgttggccaa taatcagcat gaaatttcag gcttgggtta tttggcgaca gaagctgcct
    63301 atcgatacgg taaagattgg ctagaggaac tcaagcaagt ctttgaagac cacatcaatt
    63361 atgtggtgga tctatttgga aaagagacta aaatcaaggt catgaaaccg caaggtacct
    63421 acttgatttg gcttgacttt tcagcctatg acctgactga tgaaacattg caagagttgt
    63481 tgagaaatga agccaaggtt atcctcaacc gtggtttgga ttttggagag gaaggaagtc
    63541 tccatgcccg catcaatata gctatgtcca aatctctgtt gcaagaagtc tgtcagcgga
    63601 ttgtggctac ttttgccaaa cgttaaaaat ccagccttct aggagaaaag tcttcctaga
    63661 aggctatttt cataggcgaa aatatggtat aataaaaaga taaggtaaag gtgaaaatat
    63721 ggctaaattg attccgggga aaattcgtat cgaaggtgtt gccctttatg aaactggtaa
    63781 ggttgatatc atcaaggaaa agaacaatcg gctctacgct cgcgttgcag aagaagaact
    63841 gcgctatagt ttagaggatg atttggtttt ttgtgcctgt gattcttttc aaaagagggg
    63901 ctactgtgtg catttggcag cgctagagca ttttctgaaa aatgatgagc gtggtcagga
    63961 aatcttgtgg agtctggaag aaggtcatga agaaaaagag gccgttgaaa ccaaggtgac
    64021 cttgggtggc aagtttttga atcgaatttt atctccaaaa tcagaatgcg cctatgagtt
    64081 atcagctgtg ggtcaggtgg aagcaggaac caatcatatc ttgtggactc tgagaatcgg
    64141 ccaaatcaat agccaaaaat actatgtcat tcgggatatt ccactttttt taaggattgt
    64201 cgagcagaga aagtcgtata tgattggcaa gatttatgag gagtctcttt cttgggaagc
    64261 ctttgatgat gccagccaag aacttttgac tttcttgcac ggactaacgg aggaagggct
    64321 ggttccagac ctctttttcc aaaaccaagg tcggcacctc tttttccctc tgaccttttt
    64381 tgagcagggt gtgaatttac tgatggcctt gccccatttt caatttgacc atcaagtgga
    64441 tagctaccaa accctgcttt ttcaggatat gcatgctgat gccaatctat ttgcctttac
    64501 agtaacagaa tactcggact attttgaaat ggaaatcagt gagagtccta gggtcaatgt
    64561 cttttatcag ggagcagtac ttttccataa aggtcaggtt tattttttaa cagaccagca
    64621 gatgcgtctt ctcaaggaaa ttaaagcgtt acctgtgggt caacaaggaa agaaatacct
    64681 gcaatttgat agcagtgatc gagacaaatt ggcctcctgt ctgactcttt ttatccagat
    64741 gggcaccgtt tcagctccag aacgtctaca aatcaaaact tttgcgccct ctttttactt
    64801 tgacagggaa gaagataatc ggattcgttt agaaattcag tttgattatg gaaatagaca
    64861 ggtatctagc cgacaagagc tagaagaatt accattttca agtgacgcgg acttagaaga
    64921 aagaattttc caagtctgtt tggctgcggg ctttgaagcc gattttcaat cttggcgtca
    64981 ggccttaaaa gcggagtctg tctatcattt ttttcatgat atcattccag tctttgaaaa
    65041 actcgggcat gttgacctat cagataaact agaagagctt tatagcctag cgagtcctca
    65101 ggtgcagatt gcctccaagg gaggtctctt ggaaatccag tttgattttc aaaatattgc
    65161 ccaggaggaa atcgaccaag ccatgcaggc cttggttgcc aatcaggatt tttatattga
    65221 ttcgtctaat caagtttact ttttcgatga agaaaccaag aaaattcgcc aaaatctaca
    65281 ggaactgggg caatttgaat taaaagatgg gaccttgcag gctcggaaat ccttggccta
    65341 cagtttagct catctctttg aagggcgaga ccgtgtttct ttttcacaag aattccagaa
    65401 tctggcccag gatttgacgc atccagagga ctttcctttg caagcaactc aggtcaaggc
    65461 tgacttgcga gattatcagg aaaagggaat tggctggttg cagatgctcc atcattatgg
    65521 ttttggtggg attttggctg atgatatggg acttgggaaa acccttcaga ccattgcttt
    65581 tttgactagt caagtgacaa aagaaagtcg agtcttgatt ctcgctccgt cgggtttgat
    65641 ttacaactgg gcggatgagt ttcagaaatt tgccccacag ttggatgtgg ctgttgttca
    65701 tggtttgaaa gcaagtcgtg aggagattct tgccgagagc catcaaatct atgtgactag
    65761 ctatgccacc ttccgtcagg acagtgagtt ttatcagggg atggcctttg acttcctttt
    65821 cttagatgag gctcaggtca tgaaaaatgc ccagaccaag attgcccaga ccttgagaca
    65881 atttgtggtg ccgtcggtct ttgccttgtc aggaactccg attgaaaacc atctgggtga
    65941 gttgtggtct attttccaaa tcgtcatgcc cggacttttg ccaagcaaga aagaatttat
    66001 gaaattgcca gcagagcgag tggctcagtt tatcaagcct ttcgtgatgc gacgcaagaa
    66061 agaagaagtt ctgactgaat tgccagactt gattgaggtg gtttataaaa atgaactgga
    66121 agaccagcaa aaggctattt accttgccca gttacaacag atgcgagacc atctggctca
    66181 agtgtcagaa caggaatttc agcgaagtcg tgtggaaatt ttatctggtt tgatgcgctt
    66241 gcgtcaaata tgtgacactc ctgccctgtt tatggaagat tatcagggag ccagcggcaa
    66301 actagatagt ctccgagacc tactggtaca ggtggcagac ggcggacacc gtgtcttgat
    66361 tttctctcag ttcaagggaa tgttggaaaa aattgaacaa gaactgtcag acttgggcct
    66421 gacttccttt aaaattacgg gttcaacccc agccaaggaa agacaagaca tgaccaaggc
    66481 ctttaatcaa ggagaaagag atgcctttct gatttccctc aaggctggtg gggtcggtct
    66541 gaacctgtca ggtgcagata cagtgatctt ggttgacctt tggtggaatc ctgcggtgga
    66601 agcgcaagcc attggccgtg cccatcggat gggtcaggaa gaaacggttg aggtctatcg
    66661 cttggtgacc aagggaacca ttgaagaaaa aattcaggaa ctccaagaac aaaagaaaca
    66721 tctggtgtca caagtattgg acggcacaga gtcacgtggt agtctgaccc tagcagaaat
    66781 tagagaaatt ttgggaattt ctgaagccag cacttgaaaa atcaagacaa tacgctataa
    66841 tgaagtgttg aaaggaaata gaaaatgaaa caagaacgat ttccattggt gtcagatgac
    66901 gaggtcatgt tgactgaaat gccagtcatg aatctctatg atgagtctga tctgatcagt
    66961 aatatcaagg gtgagtatcg agataaaaat tatttagaat gggctcctat tgctgaagaa
    67021 aaaccagtaa aaccgattga aaagcaagta gggaaaccta aaaaagctcc tttaggggtt
    67081 aaaaaagaag gaaagagcta tgcggaggtg gcgcgtgaag aagcgcgtgc agacttgaaa
    67141 aagaaacgct ctgctaacta tctaactcag gatttcagcc ttgcgagacg tcattctcag
    67201 cccagtctag ttagacaggg caatcaaccg acaactcctt tccaaaagga aaatcctggt
    67261 gaatttgtca aatatagcca aaaattgacc cagtctcatt atatcttggc ggaagaagtt
    67321 cattctatcc ctaccaagaa tgaagaagtg tcagcacctg ctccaaagaa aaacaattat
    67381 gattttctaa agaagagcca aatctacaat aaaaaaagta aacaaacaga acaagaacgt
    67441 cgggttgccc aagagttgaa tctgaccaga atgacagaat aggggagaaa acatgtcaaa
    67501 gacatatcat tttatcggaa ttaagggatc agggatgagt gccttggcct tgatgttgca
    67561 ccagatgggg cacaaggttc agggatcaga tgttgaaaag tactacttta cccaacgcgg
    67621 tcttgagcag gcaggaatta ccattcttcc ttttgatgaa aagaatctag acggtgatat
    67681 ggaaattatc gctggaaatg cctttcgtcc agataacaac gtcgaaattg cctatgcgga
    67741 ccaaaatggt atcagctata aacgttacca tgagtttcta ggtagcttta tgcgtgactt
    67801 tgttagcatg ggagtagcag gagcacatgg aaaaacttca acgacaggta tgttgtctca
    67861 tgtcttgtct cacattacag ataccagctt cttgattgga gatgggacag gtcgtggttc
    67921 ggccaatgcc aaatattttg tctttgaatc tgacgaatat gagcgtcact tcatgcctta
    67981 ccacccagaa tactctatta tcaccaacat tgactttgac catccagatt atttcacaag
    68041 tctcgaggat gttttcaatg cctttaacga ctatgccaaa caaatcacca agggtctttt
    68101 tgtctatggt gaagatgctg aattgcgtaa gattacgtct gatgcaccaa tttattatta
    68161 tggttttgaa gctgaaggca atgactttgt agctagtgat cttattcgtt caacaactgg
    68221 ttcaaccttc accgttcatt tccgtggaca aaacttgggg caattccaca ttccaacctt
    68281 tggtcgtcac aatatcatga atgcgacagc cgttattggt cttctttaca cagcaggatt
    68341 tgatttgaac ttggtgcgtg agcacttgaa aacattttcc ggtgttaaac gtcgtttcac
    68401 tgagaaaatt gtcaatgata cagtgattat cgatgacttt gcccatcatc caacagaaat
    68461 tattgcgacc ttggatgcgg ctcgtcagaa atacccaagc aaggaaattg tagcagtctt
    68521 tcaaccgcat acctttacaa gaaccattgc cttgttggac gactttgccc atgctttaaa
    68581 ccaagcagat gctgtttatc tagcgcaaat ttatggctcg gctcgtgaag tagatcatgg
    68641 tgacgttaag gtagaagacc tagccaacaa aatcaacaaa aaacaccaag tgattactgt
    68701 tgaaaatgtt tctccgctcc tagaccatga caatgctgtt tacgtcttta tgggagcagg
    68761 agacatccaa acctatgaat actcatttga gcgtctcttg tctaacttga caagcaatgt
    68821 tcaataggat gttcccatgg aaattccaat taagatcatt caggcaagca agtctgattt
    68881 gcctgagata ggggcacttc agacctcgtc ttttccagct gaaaagcagc aactttccca
    68941 tattttagaa gaaagtatcc gtaagtgtgc ggataccttt cttctagcta gggatgaaaa
    69001 tcaactttta ggctatattt tatcaagtcc ccagtcagac aatccgcaat atctaaaagt
    69061 acattcttta gtcatcgagt ctgaccatca gagacagggc ttgggaacac ttcttcttgc
    69121 agccttgaaa gaggtggcag ttgatctgga ttacaaaggg attcgtttgg agagtcctga
    69181 tgagctgctt tcctattttg aaatgaacgg ttttgttgat gaagaagcaa ctttgctcta
    69241 tgcaactagc cagggctata gtatgatttg gtttaatccc ttttatctgg aggaacaatg
    69301 aaaatcagac aagcaagatt agaagatttg gatcggattg ttgaacttga atttgaaaat
    69361 ttttcggtcg aagaagccat tcctccttct gtctttgaag cacatttgag agaaattcag
    69421 acctcttttc tggttgctga aaaagaagga agaatcatgg gttatatcga aggaccagtt
    69481 ggcctgcacc gccatctgca agaccagtct tttacagaag aaataaaaga ctatagtcat
    69541 gagcctggtg gttatatctc tgtgacctgt ctgtctattg ccaaggaggc acagggactc
    69601 ggactgggtc agaaattgct gacagccttg aaagaagttg ctcttgaaga tgagagagat
    69661 ggcattaatc taacctgtca tgactatctc atcgcctatt atgaaaaaca tggatttgtc
    69721 aacgaaggcc agtcccagtc aacctttgca ggggaaacat ggtatgatat ggtctgggaa
    69781 atgaaaaaat aagttaggaa aagtatcata gattgctttt tttggacttt taagatataa
    69841 tattatggat tatcaacaag gaggaaaaac ttttgagtga aaagtcaaga gaagaagaga
    69901 aattaagctt taaagagcag attctgagag atttagaaaa agtaaaaggc tatgatgaag
    69961 ttctgaaaga agatgaggca gtagttcgca ctcctgcaaa tgaaccttca gctgaagaac
    70021 tcatggctga ttccttgtca acggtagagg agattatgag aaaagctcct accgtgccta
    70081 ctcacccaag tcaaggtgta ccagcttctc cagcagatga gattcaaaga gaaactcctg
    70141 gtgttccaag tcatccaagt caagatgtac cttcttctcc agcggaagaa agtggatcaa
    70201 gaccaggtcc aggtcctgtt agacctaaga aacttgaaag agaatacaat gaaaccccaa
    70261 caagggtagc tgtttcctat acgacggcag agaaaaaagc agaacaagca ggtccagaaa
    70321 cacctacgcc tgctacagaa acagtggata tcatcagcga tacatcacgt cgtagccgta
    70381 gagaaggagc aaaacccgtt aaacctaaga aagagaagaa gtcacatgtg aaagcttttg
    70441 tgatttcatt ccttgtattc cttgccttgc tctcagcagg tggttacttt ggttaccagt
    70501 acgtgctaga ttctttgtta cctatcgatg ctaattctaa gaaatatgtg acggttggaa
    70561 ttccagaagg ttcaaacgtt caagaaatcg gtacgacgct tgaaaaagct ggtttggtaa
    70621 agcatggtct gatttttagt ttttatgcca agtataaaaa ttataccgac ttgaaagcag
    70681 gttactacaa tttgcaaaag agtatgagta cagaagactt actcaaagag ttgcaaaaag
    70741 gtggaacaga tgaaccgcaa gaacctgtac ttgcgacttt gacaattcca gaaggttata
    70801 ccttggatca gattgctcaa actgtgggtc aattgcaagg tgacttcaaa gagtctttga
    70861 cagcggaggc tttcttggct aaagttcaag atgagacgtt tatcagtcaa gcagtagcga
    70921 aatatcctac tttactggaa agtttgcctg taaaagacag cggtgcgcgt tatcgtttgg
    70981 aaggatacct tttcccagct acatactcta tcaaggaaag cacaactatt gagagcttga
    71041 ttgatgagat gttagctgct atggataaga acctatctcc ttactatagt actatcaaat
    71101 ctaaaaactt gactatcaat gagttgttga ccattgcttc cttggtcgaa aaagaaggtg
    71161 ccaagacaga agatcgtaag ctcattgcag gtgtattcta caatcgttta aatcgtgata
    71221 tgccacttca aagtaatatt gcaatcttgt atgcccaagg aaaactgggg caaaatatca
    71281 gtctagctga ggatgttgcg attgataccg acattgattc accttataat gtttataaaa
    71341 atgtaggtct catgcctggt ccagtcgata gtccaagtct ggatgcgatt gagtcaagca
    71401 tcaatcaaac taagagcgat aacctctact ttgtagcaga tgtcacagaa ggcaaggtct
    71461 actatgctaa caatcaagaa gaccacgacc gcaatgtcgc tgaacatgtc aacagcaaat
    71521 taaactaaac aaactaaaat tatgtgatac ttcacataat tttctttaga aaatattatc
    71581 agaagaaagt tgagaaaaat ggcagaaaaa acatatccta tgacccttga ggaaaaggag
    71641 aaacttgaaa aagaattaga agaattgaaa ttggttcgtc gaccagaagt ggtagaacgc
    71701 attaagattg cccgttcata cggtgatctt tcagaaaaca gtgagtacga agcagctaag
    71761 gatgaacaag cctttgtcga aggacaaatc tctagcttag aaacaaaaat ccgctatgct
    71821 gaaatcgtca atagcgacgc agttgcccag gacgaagtag cgattggtaa aacagtcacc
    71881 atccaagaaa ttggtgagga cgaagaagaa gtttatatta tcgtaggttc agctggtgcg
    71941 gatgcctttg caggtaaggt ttcaaatgaa agcccaattg ggcaggcctt gattggcaag
    72001 aaaacaggtg atacagcaac cattgaaacg cctgttggta gctatgatgt aaaaatcttg
    72061 aaggttgaaa aaacagccta aaaacagaaa aaggagtggg gaggcgatgt gcttcactca
    72121 ctcctttttc cattttgcta ctcttcgaaa atctcttcaa accacgtcag cgtcgccttg
    72181 ccgtatgtat ggttactgac tt
//
LOCUS       DAVBDG010000002        70160 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBDG010000002 DAVBDG010000000
VERSION     DAVBDG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 70160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.002"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            1..342
                     /locus_tag="VQ526_000074"
                     /pseudo
     CDS             1..342
                     /locus_tag="VQ526_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            345..1357
                     /locus_tag="VQ526_000075"
                     /pseudo
     CDS             345..1357
                     /locus_tag="VQ526_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182321.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            1332..1910
                     /locus_tag="VQ526_000076"
     CDS             1332..1910
                     /locus_tag="VQ526_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HET2553856.1"
                     /translation="MVRNYWKARSWLLVLALSFLILSPVGAQESLSSYFVKITDTSKA
                     VKNGNQSEAQKLVQEMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSIAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(1967..2251)
                     /locus_tag="VQ526_000077"
     CDS             complement(1967..2251)
                     /locus_tag="VQ526_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2553857.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(2305..3240)
                     /locus_tag="VQ526_000078"
     CDS             complement(2305..3240)
                     /locus_tag="VQ526_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2553858.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVSAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3344..3787
                     /locus_tag="VQ526_000079"
     CDS             3344..3787
                     /locus_tag="VQ526_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2553859.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            3903..4169
                     /locus_tag="VQ526_000080"
     CDS             3903..4169
                     /locus_tag="VQ526_000080"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2553860.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVGSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            4166..4540
                     /gene="crcB"
                     /locus_tag="VQ526_000081"
     CDS             4166..4540
                     /gene="crcB"
                     /locus_tag="VQ526_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2553861.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            4534..4863
                     /gene="crcB"
                     /locus_tag="VQ526_000082"
     CDS             4534..4863
                     /gene="crcB"
                     /locus_tag="VQ526_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2553862.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQVSKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            4982..5329
                     /gene="rplS"
                     /locus_tag="VQ526_000083"
     CDS             4982..5329
                     /gene="rplS"
                     /locus_tag="VQ526_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2553863.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            5371..5442
                     /locus_tag="VQ526_000084"
     tRNA            5371..5442
                     /locus_tag="VQ526_000084"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5404..5406,aa:Arg,seq:cct)
     gene            complement(5608..6606)
                     /locus_tag="VQ526_000085"
     CDS             complement(5608..6606)
                     /locus_tag="VQ526_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2553864.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNKLECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLKVVELKTILSKHN
                     LATSGKKAELITRIINNINVDKLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(6649..7455)
                     /gene="yidA"
                     /locus_tag="VQ526_000086"
     CDS             complement(6649..7455)
                     /gene="yidA"
                     /locus_tag="VQ526_000086"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2553865.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKSNDESGVAHAIRTWVL"
     gene            complement(7468..8772)
                     /locus_tag="VQ526_000087"
     CDS             complement(7468..8772)
                     /locus_tag="VQ526_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2553866.1"
                     /translation="MNEKVFRDPVHNYIHVINQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMIAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQSPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPKDKDFFARTSPHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN"
     gene            8844..9221
                     /locus_tag="VQ526_000088"
     CDS             8844..9221
                     /locus_tag="VQ526_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2553867.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKNSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            9312..9644
                     /locus_tag="VQ526_000089"
     CDS             9312..9644
                     /locus_tag="VQ526_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2553868.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            9656..11227
                     /gene="ffh"
                     /locus_tag="VQ526_000090"
     CDS             9656..11227
                     /gene="ffh"
                     /locus_tag="VQ526_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2553869.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(11422..12705)
                     /locus_tag="VQ526_000091"
     CDS             complement(11422..12705)
                     /locus_tag="VQ526_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2553870.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(12959..13672)
                     /gene="rsmG"
                     /locus_tag="VQ526_000092"
     CDS             complement(12959..13672)
                     /gene="rsmG"
                     /locus_tag="VQ526_000092"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2553871.1"
                     /translation="MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            13766..14326
                     /locus_tag="VQ526_000093"
     CDS             13766..14326
                     /locus_tag="VQ526_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2553872.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            14328..15227
                     /gene="htpX"
                     /locus_tag="VQ526_000094"
     CDS             14328..15227
                     /gene="htpX"
                     /locus_tag="VQ526_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2553873.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(15271..16830)
                     /locus_tag="VQ526_000095"
     CDS             complement(15271..16830)
                     /locus_tag="VQ526_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2553874.1"
                     /translation="MHYEHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIHSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLELVNLEDL"
     gene            17555..18097
                     /locus_tag="VQ526_000096"
     CDS             17555..18097
                     /locus_tag="VQ526_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2553875.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            18097..18726
                     /gene="nth"
                     /locus_tag="VQ526_000097"
     CDS             18097..18726
                     /gene="nth"
                     /locus_tag="VQ526_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2553876.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            18937..19458
                     /gene="pyrR"
                     /locus_tag="VQ526_000098"
     CDS             18937..19458
                     /gene="pyrR"
                     /locus_tag="VQ526_000098"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2553877.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            19477..20400
                     /locus_tag="VQ526_000099"
     CDS             19477..20400
                     /locus_tag="VQ526_000099"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2553878.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            20450..21529
                     /gene="carA"
                     /locus_tag="VQ526_000100"
     CDS             20450..21529
                     /gene="carA"
                     /locus_tag="VQ526_000100"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2553879.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            21842..25018
                     /gene="carB"
                     /locus_tag="VQ526_000101"
     CDS             21842..25018
                     /gene="carB"
                     /locus_tag="VQ526_000101"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2553880.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELSVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAVLAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(25293..26102)
                     /locus_tag="VQ526_000102"
     CDS             complement(25293..26102)
                     /locus_tag="VQ526_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2553881.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKYDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(26104..26907)
                     /locus_tag="VQ526_000103"
     CDS             complement(26104..26907)
                     /locus_tag="VQ526_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2553882.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(26917..28404)
                     /gene="tacF"
                     /locus_tag="VQ526_000104"
     CDS             complement(26917..28404)
                     /gene="tacF"
                     /locus_tag="VQ526_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2553883.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVVPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            28740..29447
                     /locus_tag="VQ526_000105"
     CDS             28740..29447
                     /locus_tag="VQ526_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2553884.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            29452..30474
                     /locus_tag="VQ526_000106"
     CDS             29452..30474
                     /locus_tag="VQ526_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2553885.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            30491..31360
                     /gene="licA"
                     /locus_tag="VQ526_000107"
     CDS             30491..31360
                     /gene="licA"
                     /locus_tag="VQ526_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2553886.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            31350..32228
                     /locus_tag="VQ526_000108"
     CDS             31350..32228
                     /locus_tag="VQ526_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2553887.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            32240..32929
                     /locus_tag="VQ526_000109"
     CDS             32240..32929
                     /locus_tag="VQ526_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2553888.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            33025..33873
                     /gene="dprA"
                     /locus_tag="VQ526_000110"
     CDS             33025..33873
                     /gene="dprA"
                     /locus_tag="VQ526_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2553889.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            34757..35800
                     /locus_tag="VQ526_000111"
     CDS             34757..35800
                     /locus_tag="VQ526_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2553890.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEHFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            35967..38054
                     /gene="topA"
                     /locus_tag="VQ526_000112"
     CDS             35967..38054
                     /gene="topA"
                     /locus_tag="VQ526_000112"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2553891.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            38275..38634
                     /locus_tag="VQ526_000113"
     CDS             38275..38634
                     /locus_tag="VQ526_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2553892.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            38727..39359
                     /locus_tag="VQ526_000114"
     CDS             38727..39359
                     /locus_tag="VQ526_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2553893.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            39403..40059
                     /locus_tag="VQ526_000115"
     CDS             39403..40059
                     /locus_tag="VQ526_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2553894.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            40078..40710
                     /locus_tag="VQ526_000116"
     CDS             40078..40710
                     /locus_tag="VQ526_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2553895.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            40738..41172
                     /locus_tag="VQ526_000117"
                     /pseudo
     CDS             40738..41172
                     /locus_tag="VQ526_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
     gene            41184..42221
                     /gene="leuB"
                     /locus_tag="VQ526_000118"
     CDS             41184..42221
                     /gene="leuB"
                     /locus_tag="VQ526_000118"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET2553896.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VEWIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIYGSA
                     PDIAGQGIANPISMILSVAMMLRDSFGGYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            42218..42487
                     /locus_tag="VQ526_000119"
     CDS             42218..42487
                     /locus_tag="VQ526_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2553897.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            42491..42838
                     /locus_tag="VQ526_000120"
     CDS             42491..42838
                     /locus_tag="VQ526_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2553898.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNGMLPIVQPREV
                     REKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQYEELI
                     AAYEKQRPAYWQD"
     gene            43057..43200
                     /locus_tag="VQ526_000121"
     CDS             43057..43200
                     /locus_tag="VQ526_000121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2553899.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            43300..43473
                     /locus_tag="VQ526_000122"
     CDS             43300..43473
                     /locus_tag="VQ526_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2553900.1"
                     /translation="MTDVRGTSCFVIKFGKADEQLAAKLWEEGKMVYASSTSMIKRLK
                     LAMRARCNGVYGR"
     gene            43463..43624
                     /locus_tag="VQ526_000123"
     CDS             43463..43624
                     /locus_tag="VQ526_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2553901.1"
                     /translation="MVDKNGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
     gene            43787..45718
                     /locus_tag="VQ526_000124"
     CDS             43787..45718
                     /locus_tag="VQ526_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2553902.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEKLLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYSGGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            45705..47021
                     /locus_tag="VQ526_000125"
     CDS             45705..47021
                     /locus_tag="VQ526_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2553903.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            47196..48182
                     /locus_tag="VQ526_000126"
     CDS             47196..48182
                     /locus_tag="VQ526_000126"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2553904.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            48246..48317
                     /locus_tag="VQ526_000127"
     tRNA            48246..48317
                     /locus_tag="VQ526_000127"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48279..48281,aa:Arg,seq:ccg)
     gene            48500..49198
                     /locus_tag="VQ526_000128"
     CDS             48500..49198
                     /locus_tag="VQ526_000128"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2553905.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            49189..52728
                     /gene="smc"
                     /locus_tag="VQ526_000129"
     CDS             49189..52728
                     /gene="smc"
                     /locus_tag="VQ526_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2553906.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELTQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGSSSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDVIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMATLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEKDRLSDGDWQADKENCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNFQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVRNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            52725..53519
                     /locus_tag="VQ526_000130"
     CDS             52725..53519
                     /locus_tag="VQ526_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2553907.1"
                     /translation="MTIKLVATDMDGTFLDENGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            53519..54337
                     /locus_tag="VQ526_000131"
     CDS             53519..54337
                     /locus_tag="VQ526_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2553908.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKSTLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYADVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            54341..55630
                     /gene="ftsY"
                     /locus_tag="VQ526_000132"
     CDS             54341..55630
                     /gene="ftsY"
                     /locus_tag="VQ526_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2553909.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEMEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPNSQEVTEEEKLEHE
                     GTVEENNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(55676..57163)
                     /locus_tag="VQ526_000133"
     CDS             complement(55676..57163)
                     /locus_tag="VQ526_000133"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2553910.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGIKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(57312..58052)
                     /locus_tag="VQ526_000134"
     CDS             complement(57312..58052)
                     /locus_tag="VQ526_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2553911.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(58052..60217)
                     /locus_tag="VQ526_000135"
     CDS             complement(58052..60217)
                     /locus_tag="VQ526_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2553912.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            60390..61343
                     /locus_tag="VQ526_000136"
     CDS             60390..61343
                     /locus_tag="VQ526_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2553913.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            61420..63408
                     /gene="uvrB"
                     /locus_tag="VQ526_000137"
     CDS             61420..63408
                     /gene="uvrB"
                     /locus_tag="VQ526_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2553914.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEILVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            63418..63981
                     /locus_tag="VQ526_000138"
     CDS             63418..63981
                     /locus_tag="VQ526_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2553915.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            63947..64435
                     /locus_tag="VQ526_000139"
     CDS             63947..64435
                     /locus_tag="VQ526_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2553916.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDDTIQAGGIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            64509..64976
                     /locus_tag="VQ526_000140"
     CDS             64509..64976
                     /locus_tag="VQ526_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2553917.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(64973..65488)
                     /locus_tag="VQ526_000141"
     CDS             complement(64973..65488)
                     /locus_tag="VQ526_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2553918.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(65500..66027)
                     /locus_tag="VQ526_000142"
     CDS             complement(65500..66027)
                     /locus_tag="VQ526_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2553919.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(66309..66872)
                     /locus_tag="VQ526_000143"
     CDS             complement(66309..66872)
                     /locus_tag="VQ526_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2553920.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(66856..67407)
                     /gene="coaC"
                     /locus_tag="VQ526_000144"
     CDS             complement(66856..67407)
                     /gene="coaC"
                     /locus_tag="VQ526_000144"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2553921.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETLDEKTL"
     gene            complement(67419..68108)
                     /gene="coaB"
                     /locus_tag="VQ526_000145"
     CDS             complement(67419..68108)
                     /gene="coaB"
                     /locus_tag="VQ526_000145"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2553922.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            68338..70008
                     /locus_tag="VQ526_000146"
     CDS             68338..70008
                     /locus_tag="VQ526_000146"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2553923.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT        21288 a        12587 c        15383 g        20902 t
ORIGIN      
        1 ttcaagaaac acgtggtggt cgtaaccatg catatatgac ggttgagcaa gagaaagcct
       61 ttcttgctcg ccatttgaag gctgcagagg caggagaatt tgttacaatt gatgccttat
      121 atcaggctta taaaaaggag ttaggtcgtt cctacacacg tgatgccttc tatcaactgt
      181 tgaagcacca tggttggcga aatattatgc cacgtccaga acatcctagg aaagcagacg
      241 ctcaaaccat tgtcgcgtct aaaaataaaa tcttaattca agaagacaag aaagcgcttt
      301 aaatatagta gacgttttcg taaggttcgc ttgatgtacc aaatagtacg cccatgcaaa
      361 agaatatcta ttttgttgtc ttggacctgc atacgacgga tagagataaa attatccagc
      421 tattcaagga ttggacggat tatagtgcca agttggtcga gggggaattg gttaaaaaag
      481 atggacagaa tgctctcttt cctccgagcg atactggtga gacagttggg ctcaatcctc
      541 atcgtttgac gctgactttt ggtgtatctg caagtttctt gaaaaagatg aacttggaaa
      601 acaagcgccc tcgactgttc agggatttac ctctatttcc taaggagcaa ttacgtgaaa
      661 aatatactgg tggagatatt gtgattcatg cttgtgcaga tgatgagcaa attgcctttc
      721 acgctattcg caatctcata cgtaagggga gaaatgcggt gccccttcgt tggagtcagt
      781 ctggatttgc agctatcggg gaccgaatgg agactccgcg gaacctcttt ggttttaagg
      841 atggaacagc aaatccaacc aaggagcaag actttgaccg cgttatctgg gctgatagta
      901 aggactggat ggaaaatggt tcttatatgg cagttcgtcg gattcagatg tttttagaga
      961 cctgggaccg cactggtctc taagaacaag aaaatacctt tggtcgttac aaggaaagta
     1021 gtgccccttt cggtaaaaaa atgagtttga tgaagtgaat ttgagttttt tgctagatga
     1081 ttcgcatgtt cgtttggcta agtaagtgga caagccactt ttgcgttgtt cttactctta
     1141 ctcagatggc attgatgaaa agactggtca gtttgatacg ggtttgctct tcatttcttt
     1201 ccagaaggat cctgataact ttgttaaggt tcaaactaat ctaggtgcta ctgataagat
     1261 gaatgaatac atcactcaca taggtagtgg actttttact tgctttggtg gtgtggagaa
     1321 aggagactac attggtcaga aattattgga aagctaggag ctggttgctt gttttagctt
     1381 tgtcattttt aattctttcc ccagtaggtg cccaagaaag tttgagttct tatttcgtaa
     1441 aaatcacaga tacttctaaa gcggtcaaaa atggcaatca gtctgaggcc cagaaacttg
     1501 ttcaagagat ggcatcagac ttcgaaagag tagaaaataa ggattctgag gttggtaaga
     1561 ttgttaagga aaagttagct ctatctggag atattactga agccaaactc accgaaattt
     1621 cctcagctct attagctttt gaaaaagaac aaaatcctgt tgacctagat gcggaaaagg
     1681 aaaaattggt aaaccgtcta agtcctcgtt ttgaaacctt agagcaggcc attgcctcca
     1741 aagatttgga aaaagtccga gaagctttta agaaaatgaa ttccacttgg accatcaatg
     1801 aaagtgtggt tcgtgacaat agtatagctc attatggacg tgttgaaaca gctatttcct
     1861 tcctgcctag tagcatggaa accgaaccta cagatgagtc tggaacatga aaacagcagt
     1921 tgatttcaag aaacaaaaag gaacagccga aactgttctc ttatcattat ttgagaccgt
     1981 attttttgtt gaaacgatcc acgcgtccat ctgcttgagt gaacttttga cgtccagtgt
     2041 agaatgggtg tgagtctgat gaaatttcca cacggatcaa tgggtaagtc tcgccttcga
     2101 actcaactgt ttcgttagag cgttttgttg aaccgctaag gaattggtaa ccagtagttg
     2161 tgtccatgaa gacaactggg cgatattctg gatggatatc ttttttcatt ttgcaatatt
     2221 tcctttctgc catggtctct ttgcgagcca tagattgtta ccatactagt ctatcagatt
     2281 ctgaaaagtt tggcaagtat tttatcagtt tttaagcaag ttttttaact tttggtagat
     2341 gatttcgttt tcctctaggc tataggaatt agcaccactt gctagagggt ggcctccacc
     2401 atcatgttcc ttggcaattt cattgatagg atggatttta ctgcgtaagc gaacgcggta
     2461 gtggccatca gcctgttcca caaaaattcc ccagagacta acactatcaa tacgcccagg
     2521 tgcacttaca atggctgcag tttcagcatc ggttacattg tattgtttca agacttcttg
     2581 actcaggata acgcgagcag caccattttc atccacttcc agatggtcgt agatgtagcc
     2641 ttgcagttta gcaattttgt agctcatagt gtccattttg cgagtgagag ccgcaaagtc
     2701 aaagttatgt tctctcaaat aagcagccag gcgaagagtc cgtgcagtgg tagaagggta
     2761 gaggaagcga cctgtatcac cgacaattcc tgcaaagagc aactcagcat cgcgatctgc
     2821 caaggctagt tgggttgttt gggcaaatag ggtaatcatc tcgctagcgc tacttgaact
     2881 agtatcgacc caagacaggt caccgtatac atcatcattt ggatggtggt caatcttaat
     2941 gagaaaatca ccttgactat agcgtttatc atcgatacga gcagtattag ctgtatcaca
     3001 gacgatgaca agtgcgcctt ggtaggccct atcttcaaca agatccatct cagccatcca
     3061 agtaagagtt ggttcatcaa aaccgacggc tttgatggtt ttttctggga aatgatgttc
     3121 cagcaaggct ttcaatccca cctgacttcc caaggcatca gggtctggtt tcatatgacg
     3181 atgaatgata atggtgtcgt attctttaat tttttctaaa atttgttggc aaatctccat
     3241 aaataacctc aattctttct catttcattg tagcacaaga atttttattt ttaaaatgaa
     3301 aaagatgtga tataatggag aaagataaat tgaggaggac gatatggcat tagcaaaaat
     3361 tgtatttgcc agtatgaccg gtaataccga agaaattgca gatattgtag cagacaaatt
     3421 acgtgacttg ggcttggatg ttgatgttga tgaatgtaca actgttgacg cttcagactt
     3481 cttggaagca gacatcgcta tcgttgcgac ctatacttat ggtgatggtg aattgccaga
     3541 tgagatgatg gacttctacg aagacctagc agatctcaac ttgaatggca aaatctacgg
     3601 agtggtcggc tcaggagata ccttctacga tgaattctgt aaggctgttg atgactttga
     3661 ccgtgtcttt gtgtcaacag gagcagaaaa aggttcagag tgtgttaaag ttgatctttc
     3721 tgccgaggaa gaagatattg aacgcttgga acaattcgca gaagaattgg ttgctaaagt
     3781 aggataagca taaaagtgcg ctgatgtaat caatcagtgc atttttctta tcgagagttg
     3841 ggattttact agcctaattg atggcttttt gatacaataa ttcaaataaa ggaggagact
     3901 gtatggattt agatattatt cgtcaagaaa tcgatcaaat cgacgaccaa attgtcaaac
     3961 tgttagaaga acgaatgcat ttagttgagg gggtggttgc ttataagaaa gcttcaggta
     4021 aaccgatttt agatactaag agagaagaag ttatttttga aaaggttggg agtcgtgtag
     4081 aggacaagcg ctatcaggag actattgtag cgactttttc ggacatactc aaacgttcgc
     4141 gtgattatca ggatcaaaat atcaaatgaa aaaagaacaa ttttatccgc taggaatttt
     4201 tctagctgct atgttgggcg gacttgtccg atatctagtt tccacctggt taccagccag
     4261 tccagacttt ccttggggca ctctctttgt caactatctg ggaattttct gcttgattta
     4321 tcttgtcaag ggctatctgg tctataaggg gactagtaag ggcttgattt tagcactggg
     4381 gacgggtttt tgcggaggtt taacaacttt ttccagccta atgcttgata ctgtgaagct
     4441 gcttgataca gggcgttatc ttagtttgat actgtatttg cttttgagta tcggtggagg
     4501 cctgctttta gcttactatt tggggaggaa gaaatggtaa tcgtctatct tgcaatcgcc
     4561 tgtggcttgg gagcactcgt acggtatttc ttttctcgct ataatcaagt ttctaaattg
     4621 ccactcggaa cgctcatagc taatctttta ggttgttttt taattggagt attctacaat
     4681 catgtagagt ctaaggaagt atatgctatt ctagcaacag gattttgtgg cggtttaaca
     4741 actttttcga ccttgaatga cgaacttcaa agactgctaa gtgataagaa ggtcttttat
     4801 tcttacctga ctttgactta cataggtggt ttggttgcga tttttttagg aattttgcta
     4861 taaatttctt tacttttttt tagaaatttg gtaaactgta tcttgtgtgt aatggacgca
     4921 caaaaataca gtttatccgc tgaggaagat tcctcaagat tgacaaagat aggagaataa
     4981 aatgaatcca ttaatccaaa gcttgactga aggtcaactt cgtacagata tcccatcatt
     5041 ccgtcctggt gatactgttc gtgtacacgc gaaagttgtc gaaggtaacc gcgaacgtat
     5101 ccagattttt gaaggtgttg ttatcgcacg taaaggtgct ggaatctcag aaaactacac
     5161 agttcgtaaa atctctaacg gtgtaggtgt tgagcgtatc ttcccaatcc acactccacg
     5221 tgttgaaaaa atcgaagttg ttcgttacgg taaagtacgt cgtgcgaaat tgtactactt
     5281 gcgtgctctt caaggtaaag cagctcgtat caaagaaatc cgtcgttaat tcgactaaaa
     5341 aactctgctt ttcagcagag tttttatcta gctcccttag ttcaatggat ataacaactc
     5401 cctcctaagg agtagttgca ggttcgattc ctgcaggggg cagtaattag atatcaaaga
     5461 agtttgtcaa aaagtctttg atattaaaag aacgcataat ataggggatt gtctccataa
     5521 tattatgcgt tttattattg aaaaatttgg gcaatcatca gataaaattg agactatatc
     5581 gagtatataa atgtttgtat agtcaattta ttcaaaatcg tcaagttcta aattatttaa
     5641 aggactataa cattcatatt tctttaagta attgtttatt tcttcagcag tggaacgagg
     5701 taaattaact cttaaatagt caatatcttc cttagataaa aaatgatttg cattcaaaat
     5761 tgagaagcga aataatgctt catcaaaagc aaaatcataa tcgtcatcag ataatgattc
     5821 cattaatgtt tgaatatgtt ttagtgagta gtcaggtatc gaatcgggat aatatactgg
     5881 ataatcagtg gctgaaaaat tgttttctaa tcctgaaata gtaattatca aagattctat
     5941 gtaatatatt aaagcatgtt taatattacc ttcttgttcc aaatgtcttc cctgagcctt
     6001 tcgtgtgtta gataagcatc caaaatctcc tgatacagta ttcctatgag cttgtttatt
     6061 taagagactc cattttatat caccaaaagt tgctttaaaa gagatattgt ttttttcttt
     6121 acaataatct tccatactta tgtctttatc atagtatgct ttgatatagt cactatgttc
     6181 gataataaga ttttgtgctt cttttgttag tttaaattcg aacggaatat ctaatttgtc
     6241 aacgttgata ttattaatta ttcttgtaat taattctgct ttttttccag aagtagctaa
     6301 attatgttta gataatatag tttttaattc tactacttta agatatgata aagtcttatt
     6361 tttagtgaag tatcctaatt tgtataattg attgtgtgta gattcagggt caatttgaag
     6421 agagtattta aaatatcttg ggaaattata atttctccct gttttttgag tccaatctat
     6481 gagtatgagt tgcccaggag taaggccgca ctctaatttg tttaggtaaa aattgttaca
     6541 atcagaattt gtgtctctat tatgattatt ttgttttaaa ttaaatagtt tgctaaaaaa
     6601 attcattgga ttttattgct aatcaattct tattgaaaaa tgatactttt acagtaccca
     6661 tgtacggatg gcatgggcaa cgccagattc atcattagat ttagtgatgt atttggcgat
     6721 ttttttgatt tctggatttc cattttccat gacaacaggg ttgccaacga cttccagcat
     6781 ggcacggtca ttttcttcgt ctccgatagc catggtttca tctttggtta atcctagttt
     6841 ttcagctaag tgagtaatag ctgaaccctt gtctacattc tttttaagga gttcgaggta
     6901 gaaaggagca gatttgttga tagagtagtg ctcgtaaaat tcggctggta ttttttcaat
     6961 cgcagcatcg agaatttctg gttcatcgat aaacatacat ttaacaattt ctttgccagc
     7021 catttcttca ggggtacggt agaagatagg catgctgacg agggttgatt cgtgtacagt
     7081 gtatgttccg atattgcgat ttgcagtata gataccgtcc ttggtaatgg catgcatgtg
     7141 gacaccgagc ttgcgactga ggaattccat atccagataa tcctcatagg tcaaggattc
     7201 gctgataatc tcatggccag tagcagtttc ttggacaagg gcaccattga aggtcaccac
     7261 atagtcacct tggtctctca actgcaagtc gtctagaagt ttggcaacac ctgcgatggg
     7321 gcggccagtt gcaatcacga ctttgacacc agcttctttg gcatcttgga tggcagaaaa
     7381 gacttcagga gtgatttcct tttggctgtt gacaagggtt ccgtcgatat caacggcaat
     7441 tagtttaata ctcataaatt tcctcttcta gtttttattt ggggtaaaat gatcgttctc
     7501 aatcaagtat aaaaattgct gggtaatgct agcaaagata ctgttttgat ctaacatttc
     7561 tttcggaaaa tagaaacgat tatctccgtg acgactacca gcaagggatt ggacgatagg
     7621 agacaggcta gagagttcgg ccagttctcc attcttttgt aaaatctcaa tctgtgtccg
     7681 tggattttca gattcgggac gatagatatc ataagggagg tcaaagttct tatgaatggc
     7741 agtatagtag tcgggatcaa agccaatgtc ctcaaccaat tttctcatgc tagcgagttg
     7801 gtcttgatct tcttgtgaaa aggtaatgga tttaaagacc ttgcggttga caaagcgttg
     7861 cgataaatct gcgagaattt tgtcaggact ggtcatccag agctggaagt aggtattcat
     7921 cacaccatca tctagagcca gatagtcaga caaggtcaca tttttttcaa agaaaggcag
     7981 gaggtgtggg gaagttcgtg caaagaagtc tttgtcctta gggtagagtt ccttagcgcg
     8041 cttgagaaga ttctgtagga gaacttccat agcgcgtgtt gctgggtgga aatagacctg
     8101 catatacatc tggtagcgac tgaggacata gtcttcgatg gcgtgcatgc cattgcgctg
     8161 aaaggcgata ccattttcga taggacgaat gactcggagg attcgagtca ggtcaaattc
     8221 cccataggat gctcctgtaa aataggagtc acgcaagaga tagtccatgc gatctgcgtc
     8281 aatctgacta gaaatgagtt gtacgacctg cttgttagga taggtatggt ctatgacact
     8341 ggcaactttc tctggaaaat cgggcgctac ttgtagcaag acttggtgaa tctctgtctc
     8401 aggactttga ataatctcct gagtaatagc ttcatggtct gtatcaaaga gatgttcaaa
     8461 agtatgggag taggcgccat gcccaaggtc atgtaggaga gcagcgatca tggtcaagag
     8521 agactcggca ggattccatt cctcaggata tttttcttcg aaaatctctg tgatgcgtcg
     8581 tgcaatttca tagactccta gacagtgaga gaagcgactg tgttctccac cgtggaaggt
     8641 ataactggaa gttcccagtt gtttgatccg gcgcaaacgc tgaaattctt ttgtattaat
     8701 caagtcatag atgatttgat taatgacatg gatgtagttg tgaactgggt cacggaatac
     8761 tttttcgttc atatgaccat tatagcaaaa tcctgctctt tttggtaaaa tagtaggaca
     8821 agagacaaga aagagggcct gatgtgaaga ttcggatgcg aaatacaatt cagtttgatg
     8881 agcagttgga agtgattgac cagctttatg atgtggaagt gcatgaaaaa ggagattata
     8941 gctacctgct tttctataat gaggaaaagg aaaaagtagt gattaaattt catggtcaag
     9001 aacttgtgat gagccgattt tctaatccca agaccattat gcgctttcta aagaatagcg
     9061 atagtttagc ctatattcct acacctatgg gcatgcagga gtttatcatc caaacgagcc
     9121 attatcaagt tgataggcaa aagattgaac tagattatca actacaaaat caagagggac
     9181 atccttttgc cagctatcaa ttggaaatta cttggggcta ggctcatgtc tttttcaaat
     9241 ttagctatct atcttcttgg atcggtttcc tagtgtggta gattaggcta atttttggta
     9301 taataaaagt tatggaaatc gaaaaaacca atcgtatgaa tgcgctcttt gaattttatg
     9361 cggcgctttt gacagataag caaatgaatt atatcgagct ctactacgct gatgattaca
     9421 gccttgctga aattgccgag gagttcggtg tcagtcgtca ggctgtctat gacaatatca
     9481 agcgaacaga aaagattctg gaagattatg agatgaaatt gcacatgtac tcggactata
     9541 ttgtccgcag tcagattttt gatcagattt tggagcgcta tcccaaggat aactttctgc
     9601 aggagcagat agaaatttta acaagcattg ataatagaga ataagaggaa gaaaaatggc
     9661 atttgaaagt ttaacagaac gtttgcagaa cgtctttaaa aatctacgta aaaaaggaaa
     9721 aatctctgaa tctgatgtcc aagaggcaac caaagaaatt cgcttggcct tgctcgaggc
     9781 cgacgttgcc ttgcctgttg taaaggactt tatcaagaaa gttcgtgagc gtgcagtcgg
     9841 gcatgaggtc attgatacac ttaatcctgc gcaacagatt attaaaatcg ttgatgagga
     9901 actgacagcc gttttaggtt ctgatacggc agaaattatc aagtcaccta agattccaac
     9961 catcatcatg atggttggtt tacaaggggc tggtaaaaca acctttgctg gtaaattggc
    10021 caacaaactc aagaaagaag aaaatgctcg tcctttgatg attgcggcgg atatttatcg
    10081 tccagctgcc attgaccagc ttaagacctt gggacaacag attgatgtgc ctgtctttgc
    10141 acttggaaca gaagtaccag ctgttgagat tgtacgtcaa ggtttggagc aagcccaaac
    10201 taatcataac gactatgtct tgattgatac tgcgggtcgt ttgcagattg atgagctcct
    10261 catgaatgag cttcgtgatg tgaaagcatt ggctcaacca aatgaaatct tgcttgtcgt
    10321 tgatgctatg attggtcagg aagcagccaa tgttgcgcgt gagtttaatg ctcagttgga
    10381 agtgactggg gtcatcctta ccaagattga tggcgatact cgtggtggtg ctgctctgtc
    10441 tgttcgtcac attactggaa aaccaatcaa gttcactggt acaggtgaaa agattacgga
    10501 cattgaaacc ttccacccag accgcatgtc tagccgtatc cttggtatgg gggatatgct
    10561 cactttgatt gagaaagctt ctcaggaata cgatgaacaa aaagcccttg aaatggctga
    10621 gaagatgcgc gaaaacacct ttgattttaa tgatttcatc gatcaattag atcaggtgca
    10681 aaatatgggg ccgatggaag acttgctcaa gatgattcca ggtatggcca acaatccagc
    10741 ccttcaaaac atgaaggtgg atgaacgcca gattgctcgt aaacgtgcca ttgtgtcttc
    10801 gatgacacct gaagagcgtg aaaacccaaa tttgttaaat ccaagccgtc gccgtcgtat
    10861 tgctgctggt tctggaaata cattcgtcga agtcaataaa ttcatcaagg actttaacca
    10921 ggctaaacag ctcatgcagg gtgttatgtc tggggatatg aataaaatga tgaagcaaat
    10981 ggggattaat ccaaataacc ttcctaaaaa tatgccaaat atgggaggaa tggatatgtc
    11041 tgcccttgaa ggaatgatgg gacaaggcgg tatgcctgac ttatcagctc tcggaggagc
    11101 aggaatgcca gatatgagcc agatgtttgg tggcggtttg aaaggtaaaa ttggtgaatt
    11161 tgccatgaaa cagtccatga aacgtatggc taacaaaatg aagaaagcga agaagaaacg
    11221 caagtaagac aagagattga tgcaaatctc atttgatgag aaaataaagt aaaaatttct
    11281 atgataaaac gtatagtatc aaggttttag aatatctgat attatacgtt ttttgatttt
    11341 aataattttg ttaagacact tttattatgg tatgcgaaaa atccactcag ctgtctgagt
    11401 ggattaggtg tatttagact cttagtcttt attttcgtat ggcaagatca agttcaagat
    11461 gattcctgtc atggctgaaa gggcagtacc tgaaagtgta actggaccaa gtttaaggat
    11521 agctcctcca agtccaagaa ccaacatagc acttgcgatg atgaggtttc gcatttgagc
    11581 gaaatcaaca cgttctttaa tcaagacttt caaaccattg ctggcgataa ccccatagag
    11641 aaggattgac ataccaccaa gtacagcgtt tggaatagtt gaaatcaagg cagtgaattt
    11701 accaaggaag ctgagggcaa tcgcgatgaa ggcagcgtta cggataactg agacagaagc
    11761 gatacgagtc ataccgataa cccctgtatt ttctccgtaa gttgtattgg ctggtccacc
    11821 aaggaaggca gaaacagaag ttgcgatacc gtcaccaaga agagtacggt gaagacctgg
    11881 ttcttttaag aattgacgac cacagatttg acccaaaaca gtatggtctc cgatatgttc
    11941 agaaattgtt acgatagcga ttggcaagat agcgatggct tctggaccaa agtaaagatt
    12001 gtactcttta aaggcaccac ctgtgctaaa tggcaagtag aaaccaggaa tttcgaacca
    12061 gttggcttta agaactggtg taaagtcaac caagccaaga gttagtgcga aaaggtaacc
    12121 accgataatg gcaaagagga atggaatgat tcgtaggaag ccttttcctt ttgtattgat
    12181 aaaggcagca attaggaaag taacaacggc taccagagca tttttccaat ttccgtctgc
    12241 tacaagacct gcattggtaa cagctgaacc tgcaagtcca agaccgataa cgatgatcat
    12301 aggaccgata atgattggtg gcaagagttt atcaatccat tttgttccta caaatcggat
    12361 gctggtagca acaaggacat agaccaaacc agtcaagata acccctgttt gggcagcaga
    12421 tacatccccc cccatttctt tcatagccag tgacatagct gtgataaagg caaatgaaga
    12481 acctagataa actggaactt taaaaccagt agcaatcatg tagatgagtg ttccaacacc
    12541 tgaagcaaaa agggcaacag atacaggcat tcccaaaatc aatggtacca agatggtcgc
    12601 accaaacatg gcgaaaacgt gttggaagct aaggagaatt ccttttccat ccgaaggacg
    12661 ttggtcaacg tctagtaaca aatcaacagt tgattcttgt ttcatataaa cctcactttt
    12721 gggcatcaga aaagagccca ttattttaca gcaataggcc ctcagagtaa gacttctcta
    12781 aaatctagac tttaaagagt ttcaaatatt tttgcgtctt cgtcacctca cgggatgaca
    12841 ttaaaaacct ttgcttagga tagtatagca gaaaaaaatg attttgtaaa tcattttttc
    12901 ccgagcctag aaataaaaga gcgaggctga ttttgtaaac atttgtttac taaaaaattt
    12961 aaagtgggcg tttatttggc ataccagcct tacgtggata tttatttggt gtttcttttt
    13021 tcttttctac cactgtgata tagcgcggat ctctattcgg tagggcgtag ctgagattgt
    13081 cttcgacctt actaaaaagg agattgaggg cattcttagc ttctaataat tcctcaggcg
    13141 cattgctggc cttgagtgcc aatagtttgc caccaacctt aaggtaggga atagtcaatt
    13201 cagataggac ctgcatacgg gcaaccgcac gagctgttac aaaatcatat tgagcacgga
    13261 agttcttgtc ttgggcaaaa tcttcggcac gtccgtggta gaaatgaact ccgttcaaat
    13321 ccagttcttg agccaagagt tgtaggaagt tgatgcgctt attgagtgaa tcaataatgg
    13381 tcacatctaa ctccggatag agaattttca ttggtagact aggaaatcct gccccagccc
    13441 cgatatcaag aagtttgata gtttcattgg gaatcaaacc ttgaagaatg ggtgcaatcg
    13501 aatcgtaaaa atgtttgaga taaacttctt ccttgtccgt aatcgccgtc aaattaatct
    13561 tctcattcca ctcgaccaag agctcaaaat aacgttcaaa ttgttctttt tgctggttcg
    13621 aaagtggaag attctgctcg gcaagcaagt tgtaaaatgt ttctggtttc atagttattt
    13681 ccttctttag tatcatctta ttttaccaca atcatagaaa atttgatata ctggtactaa
    13741 tctaaaagca gaaaggactt atattatgac ttggattatt cttggagtta tcgctcttat
    13801 tgttattttt gtgattgtta gctataacgg tttggttaaa aatcgtatgc aaaccaagga
    13861 ggcttggagt cagattgatg ttcagttgaa acgtcgaaat gacctcttgc cgaacttgat
    13921 tgagactgta aaaggttatg ccaaatatga aggttctacc cttgaaaagg tggcagaact
    13981 acgtaaccaa gtggcggcag cgacttcacc agcagaagct atgaaagcca gtgatgccct
    14041 cactcgtcag gtttcaggta tttttgcagt tgcagaaagc tatccagatt tgaaagctag
    14101 tgctaacttt gttaaattgc aagaggagtt gacaaacaca gaaaataaaa tttcttactc
    14161 tcgtcaactc tataacagtg ttgtcagcaa ctacaatgta aaattagaaa ctttcccgag
    14221 caatattatc gctggaatgt ttggatttaa agcggcagat ttccttcaaa cacctgaaga
    14281 ggaaaagtcg gttcctaaag ttgattttag cggtttaggt gactaagatg ttgtttgatc
    14341 aaattgcaag caataaacga aaaacctgga ttttgttgct ggtatttttc ctactcttag
    14401 ctcttgttgg ttatgcggtt ggttatctct ttataagatc tggacttggt ggtttggtta
    14461 tcgcactgat tatcggcttt atctacgctt tgtctatgat ttttcaatcg acagagattg
    14521 tcatgtccat gaatggagcg cgtgaggtgg atgagcaaac ggcaccagac ctctaccatg
    14581 tagtggaaga tatggctctg gtcgctcaga ttcctatgcc ccgtgttttc atcattgatg
    14641 atccagcctt aaatgccttt gcgacaggtt ctaatcctca aaatgcggct gttgctgcga
    14701 cttcaggtct actagctatc atgaatcgtg aagaactaga agctgttatg ggacatgaag
    14761 tcagtcatat tcgtaattat gatatccgta tttcgactat tgcagttgcc cttgctagtg
    14821 ctatcaccat gctttctggt atggcaggtc gtatgatgtg gtggggtgga gcaggtcgca
    14881 gacgaagtga tgatgaccga gatggaaatg gtcttgaaat cattatgcta gtggtttccc
    14941 tactagctat tgtactggca cctctcgctg caaccttggt tcagctcgct atttctcgtc
    15001 agagggaatt tctggcagat gcatctagtg ttgagctgac tcgcaatccc cagggaatga
    15061 ttaatgccct agataagttg gacaatagca aaccgatgag tcgccacgtc gatgatgcta
    15121 gcagtgctct ttatatcaat gatcccaaga aaggtggggg gttccaaaaa ctcttttata
    15181 cccacccacc tatctcagaa cggattgaac gtttaaaaca gatgtaaaag aatccagagg
    15241 tcgtctctgg atttttgcta tgttgcaaat ttataaatct tctaaattaa ctaactccaa
    15301 accgtgttct gtcatgcgat agatgatcga gcagacttct tttaagaaac gacggtcatg
    15361 cgaaacagtg atgagaccgc ctggataggt tgcaaagagt tttctgattt ggggttgaga
    15421 agtgggagaa aagtttcgtg tgggttcatc cagcaggaga aagtttggtt tgcgcaggac
    15481 tagatccaaa agcaggagtt ttccctgttg tccgccagat aaggagcgaa tttgatgctg
    15541 catttctgga taactgaaat tgagactggc taggtgagag tggattttct gaagttcctc
    15601 tttttcccca gttttgctga tataagctat tggtgataaa tccaattgta gttttttgtg
    15661 gtaatcttgt ggcataaaac caagggaaat ctctctttta tcattcagaa gttgctttaa
    15721 tttggctaac agagttgatt tcccaacacc atttggcccg ataataccga ttttttcttg
    15781 cccacggaca gttagttgta gtttttgaac caaaactcgg tcgtcaatgg acaaattttc
    15841 tttttccagt tggactaaga ctttagaagc tggtaatggt tggatgtctg aaaagaaaag
    15901 ttggatttgt tcctcttcaa gtggtttttg agtcatggac tgagctgcct tttcgtagcg
    15961 tttttcttgt gagaggacag ttttcatctt tttagccaat aggcgaccgg cagtactatc
    16021 tttggtagct cgaagcgcag tttctacatt ttgcttaact ctccgatgtt tttccatggt
    16081 tttatcgtag gctctttggt tgttagcagc ttgctgactt tgtttggcaa aattagcctt
    16141 tctctgctca ctatagctat catagtctaa atgctctact agcgtttccg cttctttacg
    16201 gtgtttgacc agtcgcaagt gaacaatagt gtctgccgtt tcagaaagaa agtcttcatc
    16261 atgggaaatg aaaataacgg tttgcctggt cttttgaatc tggcctttta gccaatcaac
    16321 tgtctcaagg tctaggtcat ttgaaggttc atctaaaaat agaatctcaa aaggtttggc
    16381 taactcatgg ataagctgaa ttttcaaagc ttctccccct gatagattgc caatctcttg
    16441 gtcacttgcg aaacgattgc tatcaaaatg caattcctcc gccaaacgat agaggatact
    16501 gtagtctaaa tcaatagaat ctaaaaagaa gtagtcgtgt aaagttttct ttttcagctc
    16561 ctcgggaagt ttttgaggaa tgtaggccag tgactgatag tcagattgga tgtttccctt
    16621 gatagtgaaa tcagacaaag cttcccccat taaagtttta agtaaggttg atttaccatt
    16681 tccttcttcg ccaataatag ctaccttttc cccgtcttgt atggtcatgc ttaggtcaga
    16741 gacaaggtct cgtaaatctt tgttttgtgt gatggtcagg tgattgattt ttatcatact
    16801 attgcccttt ctagaatgtt catagtgcat aacaaaaagc tctactttac ctagtagagc
    16861 ccaaagtcta tgtcaaaaaa actctattaa catgccagaa aaagggcata aaagagcaca
    16921 caaaaagaga caaaaacaag atctactaaa taaaggttct ttatttgata gacttagttg
    16981 ttcatgtctc ccttacctcc tagtattagt atctttatac tatactttta aggaaatttt
    17041 gtcaacagaa aactggaaat tctatgctct aaaatccaaa aacaggtcta tagagactta
    17101 gtacgtgttt gacatgctcg taatggattt tatcatagtt tcgccaagca gtgcgttctt
    17161 gattgtttag ttttaggcta cccagtccaa tcataggact ggtgggttgg taaaatttct
    17221 caagatcaat taagatactg ttgttcaact tttccgtttt ttgagttctt gcatagtagt
    17281 gtttagcagc tcttgtagtc gaatatcatc tgccgtaccc tgtttacagt tttggtagct
    17341 tttatttaac ttggatagga gttgaatgag tttcttgtct tccgtgtgag catcctcctt
    17401 gatctgatgt actctttccc tatagtatag cactaaaaaa taaaactgtc atagtgaata
    17461 tgttaaaatt tcgaatttta attacaaagt ttggtgtgct gatttgtagc cttttcatgg
    17521 tataatcaag agagtaaatc tttatggagg aagtatgaaa ttaaatattc aagaaattcg
    17581 taagcagtct gaaggtttga actttgaaca aacgttagat ttagttgatg acctgcgtgc
    17641 acgtaatcaa gaaattttag atgtaaaaga tatccttgca gttgggaaag tacaatatga
    17701 agaccgtatg tatttcttag attatcaact atcttatacc attgttcttg cttcgagtcg
    17761 cagtatggag ccagttgagt tagttgaatc ttatccagtc acggaagttt tcatggaagg
    17821 cgcaactaac cagctagatc aagaagtttt agatgatgac ttggtcttgc ccatcgaaaa
    17881 tggggagctt gaccttgctg agagtgtatc agacaatatc ctgctaaaca ttcctatcaa
    17941 ggtcttgacg gctgaagaag aagctggtca aggatttgtc tcaggaaatg actggcaaat
    18001 catgacagag gaagaatacc aagctcaaca agcagtaaag aaagaagaaa acagtccttt
    18061 tgctggctta caaggactat ttgacggaga tgaataatgg tcttgtcaaa aaaacgagca
    18121 cgaaaggtgc tagaagaaat cattgccctc ttcccagatg ccaagcctag tcttgatttt
    18181 accaatcatt ttgaactcct ggttgcggtc atgttatcag cccagacgac agatgcagcg
    18241 gtaaataagg ccacaccagg tctctttgtt gcctttccaa caccccaagc tatgtctgta
    18301 gcgacagaga gtgagattgc ttcacatatt tctcgcttgg gattgtatcg gaataaagct
    18361 aaattcctta aaaaatgtgc ccaacagtta ctagacgatt ttgatggtca agtccctcag
    18421 acacgtgaag aattggagag tttggcaggt gttggtcgca agacagccaa tgttgtcatg
    18481 agtgtaggat ttgggattcc agcctttgca gtggatactc atgtggagcg tatttgcaaa
    18541 caccacgata ttgtcaaaaa atcagcgacg ccacttgagg tggaaaagcg ggtcatggat
    18601 atcttgccgc ctgagcagtg gttagctgcc catcaggcca tgatttattt tggaagagcc
    18661 atttgccatc caaaaaatcc agagtgtgac cagtacccac aattatatga ttttagcaat
    18721 ttgtaagatg ctacattgaa actttttgct tgattttaag catgctttgt gctatagtaa
    18781 atgataacta aatattcctt taaacctgtc ccgtgaggca ggcaaggagc gcgataaagt
    18841 agaagggtga agtctatact gtgtgcagta gggctcgctt ggctataaat ttcaagtctc
    18901 cttgtttaag gagacttttc tttttggagg tttgtcatga aaacaaaaga agttgtagac
    18961 gaattgaccg tcaaacgagc gattacgcgt attacttatg agattatcga acgcaacaaa
    19021 gatttgaata agattgtctt ggctggtatt aaaactcgtg gtgtctttat tgcccaccga
    19081 atccaagaac gtttgaagca gttagaaaat ctttcagttc ctgttgtgga attggatact
    19141 aaacctttcc gtgatgatgt taaaagtgga gaagatactt ctttggtttc tgtcgatgtg
    19201 acagaccgcg aagttatctt ggtggatgat gtgctctata caggtcgtac catccgtgct
    19261 gctattgata atattgtcgg tcatggtcgt cctgcgcgtg tgagtttagc agttctagtc
    19321 gatcgtggac atagagaatt gccaatccgt ccagattacg ttggaaaaaa tatcccaacc
    19381 agtcgttctg aagaaatcat cgtagagatg acagaacttg atgaccaaga cagagttctg
    19441 attactgaag aagcttagaa agttaaagga gtagccatgt cagaaaatca acaagcattg
    19501 aaccatgtgg tgtccatgga agacctcact gtcgatcaag taatgaaatt gatcaagcga
    19561 ggaattgagt ttaaaaatgg agcccagctt ccctatgaag accatccgat tgtttccaat
    19621 ctcttctttg aggattctac acggacacat aagtcctttg aagtcgcaga gattaaactt
    19681 ggattggaac gacttgactt tgatgtgaag actagttcgg ttaataaggg tgagacactt
    19741 tatgacacca ttttgactct gtctgcttta ggagtggatg tctgtgtgat tcgccatcca
    19801 gaggtcgact actatagaga attgattgct agtccaacga ttacgacttc catcatcaat
    19861 ggtggagatg gttcgggcca acaccctagc cagagcttgc ttgatttgat gaccatttat
    19921 gaggaatttg gccactttga gggtcttaaa gttgctattg caggtgactt ggaccactca
    19981 cgcgttgcca aatccaatat gcagattttg aaacgcttgg gatctgaact ctttttcgct
    20041 ggacctgagg aatggagaag tcaagagttt gcagactatg gacagtttgt aaccattgat
    20101 gaaatcattg atcaggttga tgtcatgatg tttctccgtg tgcaacacga acgccatgat
    20161 agtggagctg tattttcaaa agaagactac catgcccaac atggcttgac tcaagaacgt
    20221 tacgatcgtt tgaaagaaac agcaatcctc atgcacccag ctccaatcaa tcgtgatgta
    20281 gaaatagcag accacttggt tgaagcacca aaatcacgga ttgtccaaca aatgaccaat
    20341 ggtgtctttg ttcgaatggc aatcttagaa tccgtactag cgagtagaaa cgccaactaa
    20401 aacacaagac gttaaaagac gccagtgagc gtccacgaga ggtgaaaata tgacaaaaag
    20461 aattctagta ttagaagatg gcacagtttt tgaaggcaag gccttcggag cagatattga
    20521 tgtaacaggc gaaatcgtct ttaatacagg gatgaccggc taccaagaat ccattacaga
    20581 ccagtcttat aatggacaaa tcttgacctt tacttatcct ttggtcggaa actatggaat
    20641 taaccgtgat gattacgaat ctattattcc aacttgtaag ggagttgttg ttttcgaaga
    20701 agcgcgtaga gcaagcaact ggcgcaacca aatgaccttg gatgaatttt tgaaagccaa
    20761 gaaaattcct ggtatttcag ggattgatac gcgtgcactt accaagatta ttcgtaagca
    20821 tggtactatg cgagcaacct tgactcatgt tggagatagt atggaccatg tgactgacca
    20881 gctccaagca acagtattgc cgacagataa tattaagcag gtttctacta aaacttccta
    20941 tccagctcca ggagttggtt tgagcgtggt gttagtagac tttggtctca agcactcaat
    21001 cctacgtgaa ctttctaagc gcaactgtaa tgtgacggtt gttccttatt cgacaacggc
    21061 tgaagaaatt ctccatctca atcctgacgg agtgatgttg tcaaacggtc caggtaaccc
    21121 agaagatgtt ccacaggcac tggatatgat tcgcggtgta caaggaaaaa ttccaatctt
    21181 tggtatctgt atgggacatc aactctttgc tatggcaaac ggggctaaga cctataagat
    21241 gaagtttggc caccgtggat tcaaccatgc ggtacgtgaa atcgcaacag gacgagtaga
    21301 cttcaccagc cagaaccatg gttatgcagt cagccgcgag gatttgccag agcacttgat
    21361 tattacccac gaagaaatca atgacaagtc agtggaaggt gtgcgccaca gataccaacc
    21421 tgctttctct gttcaatacc acccagatgc agctcctggg ccacacgacg ctagctacct
    21481 atttgacgag tttatcgaga tgatggaagt ttttaaacaa tcaaactaag aacgagtaaa
    21541 agctcgtcgg agaatttatg taactgaaca cggacggaaa gctcagaatt tagagaaacc
    21601 aacttggatt gtcaatcctt ccttgatttc ttaaaattca atcgctttct attcgtcctt
    21661 tgtatcttat ttaatgtcgc cctgagaggc gacgaataga aaggagaagg gtgctccgta
    21721 tttgctgaac tgaatacggg ctacggacgg tgctaaaaag atagttattc ctagaactga
    21781 cagttcttcg tcataactcc tattttagct ttgtccgctt gacgccctta atatcttata
    21841 tatgcctaaa cgtactgata ttcaaaaaat tatggtgatt ggttctggtc cgattattat
    21901 tggtcaggct gctgagtttg actatgctgg gacccaggct tgcttgtcgt tgaaagagga
    21961 aggttatgag gttgttttgg ttaactcaaa tcctgcaacc atcatgacgg acaaggaaat
    22021 tgctgacaag gtttacattg aaccgattac acttgagttt gtgacacgta ttcttcgtaa
    22081 ggaacgtcca gatgccttgc taccaacact cggtggtcag acaggtctca acatggccat
    22141 ggaattgtct aaaaatggta tcttagatga gcttagcgtt gaacttctgg gtactaaatt
    22201 gtctgccatt gaccaagcgg aggaccgtga cctctttaaa caattgatgg aagagcttga
    22261 gcaacccatt ccagaatctg aaattgttaa cacagtagaa gaagcagttg cctttgcagc
    22321 gacaattggc tacccagtca tcgttcgtcc agcctttacc cttggtggta ctggtggtgg
    22381 tatgtgtgcc aacgagaaag aattgcgtga aatcactgaa aatggtttga aattgtcacc
    22441 tgttacccaa tgtttgattg agcgttcgat tgctggtttc aaggaaattg aatacgaagt
    22501 gatgcgtgac tcagctgaca atgccttggt tgtttgtaac atggaaaact ttgacccagt
    22561 tgggattcac acgggggatt ccattgtatt tgcccctgcg caaaccatgt cagactatga
    22621 aaaccaaatg cttcgtgatg cgagcttgag cattattcgt gccctcaaga ttgaaggtgg
    22681 atgtaatgtt cagctagctc ttgatcctaa cagcttcaag tattatgtta tcgaagtaaa
    22741 ccctcgtgta tcacgttcgt cagcccttgc ttctaaagcg acaggatatc cgattgctaa
    22801 gttggctgcc aagattgcag taggtttgac cttggatgag gttataaacc cagttacagg
    22861 ttcaacctat gccatgtttg aaccagccct tgactacgta gttgctaaga ttccacgttt
    22921 cccatttgac aagtttgaaa agggtgaacg ccgtctcggt acacaaatga aggcgactgg
    22981 agaagtcatg gcaatcggtc gtaacatcga ggaatctctt ctcaaggcct gccgctccct
    23041 tgaaattggg gtgcaccaca atgaaatacc tgaacttgca gcagtttctg atgatgcctt
    23101 gattgaaaag gttgtcaaag cccaagatga ccgtctcttc tacgtatcag aagcaattcg
    23161 tcgtggctac acacctgaag aaattgcaga gcttacaaaa attgatatct tctatcttga
    23221 taaactcttg catatctttg aaattgagca agaattgggt gctcatccac aagatctaga
    23281 agttttgaaa acagccaaac ttaatggttt ttcagaccgt aagattgctg aactctgggg
    23341 aacgactgat gataaagttc gccaacttcg cttggaaaac aagattgttc cagtttataa
    23401 gatggtagac acttgtgcgg cagagttcga ctccgaaacg ccatatttct attcaaccta
    23461 tggttgggaa aatgaatcta tcagatctga taaagaatct gtacttgtcc taggttcagg
    23521 tccaatccgt atcggtcaag gggttgagtt tgactacgca accgttcact ctgttaaggc
    23581 tatccaggca gctggttatg aagctatcat catgaactca aacccagaga ccgtttcaac
    23641 agacttctct gtatcagata agctttactt tgagccattg acattcgaag atgttatgaa
    23701 cgtcattgac ttggaacaac caaagggagt tatcgttcag ttcggtggtc aaacagccat
    23761 caaccttgcg gagccattgg caaaagcagg tgtgaccatc cttggtacac aggttgctga
    23821 cctagaccgt gccgaagacc gtgacctctt cgagcaagct ctcaaagaat tggatattcc
    23881 acagccacca ggacaaacgg ctaccaatga agaagaagca gttcttgcag ctcgtaagat
    23941 tggcttccca gttcttgtcc gcccatctta tgtacttggt ggacgtgcca tggaaatcgt
    24001 tgaaaacgaa gaagaccttc gttcttacat gcgaactgct gttaaggcta gtccagacca
    24061 tccagttctt gtcgactctt atatcgttgg gcaagagtgc gaagttgatg ccatttcaga
    24121 tggtaaaaat gtactcattc ctggtatcat ggagcatatc gaacgtgctg gtgtccactc
    24181 aggtgactca atggctgttt acccaccaca aaccttgtcg caaaaggttc aggagaccat
    24241 cgcagactac accaaacgct tagcaatcgg tcttcactgc cttggaatga tgaacatcca
    24301 gtttgttatc aaggatgaaa aagtctacgt tattgaggtc aatccacgtg ccagccgtac
    24361 cgttccattc ctatctaagg taaccaatat tcctatggct caagtagcga ccaagctcat
    24421 tcttggtcaa agtttatcag aacttggcta ccaaaatgga ctttaccctg aaagcacccg
    24481 tgttcatatc aaggcacctg tcttctcctt taccaaacta gctaaggtag acagcttgct
    24541 cggtcctgaa atgaagtcaa caggtgaagt tatgggttct gatgctactt tggaaaaagc
    24601 tctctataaa gcctttgaag cttcttatct acacttgcca acttttggta atgtcgtctt
    24661 taccatcgct gatgatgcca aagaagaagc cttgaacttg gctcgtcgtt tccaaaacat
    24721 tggttatgga atccttgcga cagaagggac agcagccttc tttgctagtc atggcttgca
    24781 agcccaacct gttggtaaga ttggtgatga cgataaggat atcccaagtt ttgtacgcaa
    24841 aggaagaatt caagctatca ttaacacagt aggaacaaaa cgaactgctg acgaagatgg
    24901 tgagcaaatt cgtcgttcag ccattgaaca cggggtgccc ctcttcacag cgctagatac
    24961 agctaatgcc atgcttaagg tacttgaaag tcgtagcttt gttacagaag cgatttaaat
    25021 agaaaaataa gatatagcag actgaattta gaacagtaca cagggatact aaaatatttc
    25081 tagaaattaa tttgaatttt ctaattgatt cgttcgcatc ttatttcaat ctactataga
    25141 aaaaagtctt taaaattata aaacgcatca tatcaaggtt tttcaaaaac cttgatatga
    25201 tgcgttttat tgtgggaata tgtatttcat tttctactaa aattatgttt ttgaataacc
    25261 tctatcttag tagtttgtat aatacccctc aatcagtttt tgcgataagc tttaatgcta
    25321 tgactatacc actcttgcat ttcttttgat ggtggtgtca tataatcgcc atacatctgg
    25381 gttaaaaatt ggtcatattt tttgggaaca ggcaacatac ggccctcaaa ctcagttaaa
    25441 atcagttctt taaaggtatc aactgggaag atttctttca tcccttcctt accgatccca
    25501 actcctcctt catattgagg agtgttggtt acagcatttt tgactagttg atcaattttc
    25561 ttgtaaaagt agcgaggatt gacaaatcgg agagcgtacc agctacataa tctaagaaaa
    25621 tcttttagtt tgctatcacc gtgaactgct cgtgattttt tgatataagc tagttgacga
    25681 agggccacat acttatagct cttgtcgaca atgctcaagt ctgtaaatcg atcaattggg
    25741 aagacatcga tgaaaaggct ggtatcatga cgcttgtact taacatggtc ttctataaca
    25801 gtagaagtgt ccaaaatcga tgcgaaatta tggaagtacc aagaagatgt atcgtaggaa
    25861 agaaccttgt agcgagggtg attttcttct tcaataatct tcagtaaacg ctcataatcc
    25921 tcacgataaa gggaaatatc aatatcatca tcccaaggaa tcataccttt gtggcggatg
    25981 gctccaagca tggttccata actgagaaaa taaggaatat catatttctt acaagtctca
    26041 tcaatatagt ccagcagggc tagttgaatt tctttaattt cttttttttc taaatattgc
    26101 atcctaatcc tccaatttat aagcgtgaaa ttcatgactg tagaagcgtt tttcttctgg
    26161 tggtagggtc atataatctc cataaaattg tgtcaaaata gtatcaaatt tttcaggtgc
    26221 aggaaggctt aaattctcaa agggtaaatc gattgtttta tcaaaggtac cacttgggaa
    26281 gacttccttt tccttaaatt ttgaagggat aaaagccata tattgcccat tttcacgact
    26341 atatttttga atttctttct cgattttatt tgcaaaataa cgaggagaaa ccggtcgaag
    26401 gagtaaccag aaggctgttc gtatccaatc ttttaaaagg ctatccttat agacaatatt
    26461 tttatgttta ctgaaagaca gcagtttgaa gctttccagt ttataacaag tatcaatgac
    26521 cttaggatca tcaaagcgat ctatagggaa aatatcgata aagataccag actcataggt
    26581 ttttgtattt cgagtatcaa taattttagt cgtactgtcg gttatcttga taaagttgtt
    26641 aaagtagttc ttatcagttt ctaaggatag gagcttatac ttgctttttt ccttttgaaa
    26701 aatgttaata aatcgttggt agtcttctct aggcatggat aaatcaatat cgtcgtccca
    26761 agggataaag ccctcatgtc gaaccgcccc aatcagagta ccgtagttaa taatatagtt
    26821 gatattgtgc tttttacaga gagtatcaat ataatccaaa atttctaatt caatttgttt
    26881 ggcatcttca atggttagtt gtttcatttt aaactcctat gattttttga atttattttt
    26941 taaggctagg acatggttta aaaattcata gaaaatgcta tcttttgtga agacaagtag
    27001 actaatataa gagatagctg atagcaagac aatcaaacca gtattaatta aaaatggcaa
    27061 attaatgacc atatccacag gatacacgaa attaatcagg aaataaattg ctacaaagga
    27121 aagtgaaaag agagagtatc gaacagtata gctaaagata tgtcccaagt ggatgagttg
    27181 ttttctatgg atgaaaataa tatagaaaac aagtagagaa gtctctgata acatagttgt
    27241 cagtaagtag tattcaggaa ccacgatatg gttgaaaaag aggagactat tcaagcccaa
    27301 attgagtagc ccagcaaaga ctgtatagac tgtgatacgt ttttcatagc cattggtaaa
    27361 gagaatttgg gaaccaagaa tggtatctaa ggccaggata atcgtacgaa aagcgaagag
    27421 agaggtcaag atgccgcctc cgatatattt ttcactaccg taaagtagga tggcatttgg
    27481 tcctaaaacc atgagtccaa aactcagtgg aatgataaag aagttaaaga ttcgactacc
    27541 tctattaacc agagaaacat aggcttcttt gtctcctttc cccagatagt aactgagacg
    27601 aggcacactc actccaattg cacctgttac aaccccagct ataacggtca caattcgctg
    27661 agctatggta tagtaactaa cgttgacatc aatccctgtt ttaacgagga agaggcgatc
    27721 taaaaaagtg aagagcatat tggcattggc aaagactaac atggctgtca gagggagaaa
    27781 gagtggttta aaatcactta ggtgaatctt aacaagtttg atgtctcttt taatccaaaa
    27841 ataactaatc aggtagttaa tcagcgtcga taaactcatc acaagtgtat agacaacaat
    27901 atcgtgttca tttttaacaa ataagaaaat agagaccagc atcaggatac ggatgaaggc
    27961 agttttgtaa aagagaaaac tgtaattttc cagagcttca ttgacccatt cgattgaaaa
    28021 aatctgggca atgagttgaa tccccataac aaggtagacc tttttgacga ttggattatc
    28081 aataaagaag agaggatagg ctaggatata gacagcagtg gtcaaaatcg tacaagcgat
    28141 gcacaaataa aaaagactag aaaaggttct gttaagatct tttttgttat ccttgacatt
    28201 actgatagcc cttaaaccgt agttatagac accataagtt gcaaagggca agaaaaatga
    28261 caaaatagtg tcgactgagt tgaagtaacc atagtcagtt cggtccaaga cacgcgcgac
    28321 ataggttcca gttaggatgg gaaaaataat attcaagaca cgaattccca tgtaagatag
    28381 agcatttaat tttatacttt tcattcaatt tacctcgttt ttcattatat cataaagtta
    28441 gctaataaga aatgaagggc agtaagtcaa gtaatcactt tgaagtttca aatcttaagt
    28501 tttaagtttt ctttaaggaa agtatattat tctgaaggac tctaaaattt cgcagccatt
    28561 tattagtaat tgctacagaa ttcctagtca ttactagaaa tggactagtt tctttgaata
    28621 atagaactgc ataattctcc tattctagaa ggggaggacc agtatttctt ttatgatagg
    28681 actagattgt ggtataatag agagaataag tttttttagt aagacaaagg agaaaataga
    28741 tgatttatgc aggaattctt gccggtggaa ctggcacacg catggggatc agtaacttgc
    28801 caaaacaatt tttagagcta ggtgatcgac ctattttgat tcatacaatt gaaaaatttg
    28861 tcttggagcc aagtattgaa aaaattgtag ttggtgttca tggagactgg gtttctcatg
    28921 cagaagatct tgtagataaa tatcttcctc tttataagga acgtatcatc attacaaagg
    28981 gtggtgctga ccgcaataca agtattaaga aaatcattga agccattgat gcttatcgtc
    29041 cgcttactcc agaggatatc gttgttaccc acgattctgt tcgtccattt attacacttc
    29101 gcatgattca ggacaatatc caacttgccc aaaatcatga cgcagtggac acagtggtag
    29161 aagcagttga tactatcgtt gaaagtacca atggtcaatt tattacagat attccaaatc
    29221 gtgctcacct ttatcaagga caaacacctc aaacattccg ttgcaaggac ttcatggacc
    29281 tttatggatc tctttctgat gaagagaagg aaatcttgac agatgcatgt aaaatctttg
    29341 tgatcaaagg aaaagatgtg gccttggcca aaggtgaata ctcaaatctg aagattacaa
    29401 ccgtaacaga tttgaagatt gcaaaaagta tgattgagaa agactagtaa aatgattaat
    29461 caaatttatc aactaactaa gcctaagttt atcaatgtca aatatcagga agaggctatt
    29521 gaccaagaga atcatatcct tatccgtccc aactacatgg ctgtctgtca tgcggatcag
    29581 cgttactatc agggaaaacg tgatcccaag attttgaata aaaagcttcc aatggcaatg
    29641 attcacgagt catgtggaac cgtcatttct gacccgaccg gaacctacga ggttggtcaa
    29701 aaagttgtca tgattcccaa tcagtctcct atgcagagtg atgaagaatt ctatgaaaac
    29761 tacatgacag ggacccattt cttgtctagt ggatttgatg gctttatgag agagtttgtt
    29821 tctctcccta aagatcgtgt ggtggcttat gatgctattg aagatacggt tgcagccatt
    29881 acagagtttg tcagtgtggg catgcacgct atgaatcgtc tattgactct tgctcatagc
    29941 aagcgggagc ggatcgccgt tattggagat ggaagtttag cttttgtggt tgccaatatt
    30001 atcaactata ctttgccaga atcagagatt gtggttattg gtcgtcattg ggaaaagttg
    30061 gaactcttct catttgccaa agaatgctat attacggata atattcctga agagttggcc
    30121 tttgaccatg cttttgaatg ttgtggtggt gatggtactg gaccagctat taatgacttg
    30181 attcgctaca ttcgtcctca gggaacgatt ctcatgatgg gagttagcga atataaagtc
    30241 aatctcaata ctcgcgatgc cttagaaaag ggcttgattt tggttgggtc atctcgttct
    30301 ggtcgcattg attttaaaaa tgctatccaa atgatggaag tcaagaaatt tgccaatcgt
    30361 cttaaaaata tcctttatct agaagaacct gtaagagaaa ttaaagatat tcatcgtgtc
    30421 tttgcaaccg atttaaacac agcctttaaa acagtgttta agtgggaagt ataagtactg
    30481 gaggttaatt gtggagaaaa tcattaaaga aaaaatttct tccttactta gtcaagaaga
    30541 ggaagtcctc agtgttgaac aactgggtgg aatgaccaat caaaactatt tggccaaaac
    30601 aacaaataag caatacattg ttaaattctt tggtaaaggg acagaaaagc ttatcaatcg
    30661 acaagatgaa aagtacaatc ttgaactact aaaggattta ggcttagatg taaaaaatta
    30721 tctttttgat attgaagctg gtatcaaagt aaatgagtat atcgaatctg cgattacgct
    30781 tgattcaacg tcaatcaaga ccaagttcga caaaattgct ccaatattac aaactattca
    30841 tacgtctgct aaggaattaa gaggagaatt tgctcctttt gaagaaatca aaaaatacga
    30901 atccttgatt gaagaacaaa ttccttatgc caactatgaa tctgttagaa atgcagtctt
    30961 ctccttagag aaaagactgg ctgacttagg tgttgacaga aaatcttgtc atatcgattt
    31021 ggtgcctgaa aactttatcg aatcacctca aggacgactt tatttgattg actgggaata
    31081 ttcatcaatg aatgatccaa tgtgggattt ggctgccctc tttttagagt ctgaattcac
    31141 ttcccaagag gaagaaactt tcttatctca ctatgagagt gaccaaacac cggtttctca
    31201 tgaaaagatt gctatttata aaattttaca agatactatt tggagtctat ggactgtcta
    31261 taaggaagag caaggtgaag attttggtga ctatggtgtg aatcgttacc aaagagctgt
    31321 taaaggtttg gcttcttatg gaggttcaga tgaaaagtaa aaacggagtt ccttttggcc
    31381 ttctctcagg tattttctgg ggcttgggtc taacggttag tgcttatatc ttttcgattt
    31441 ttacagattt gtcacccttt gtggtggctg cagctcatga ttttttgagc atctttatct
    31501 tactagcttt tctcttggta aaagaaggga aagttcgcct ctcaattttc ttaaatattc
    31561 gcaatgtcag tgttatcatc ggagccttgc tagcaggccc tatcggtatg caggccaatc
    31621 tttatgcagt taagtatatc ggaagttctt tagcttcatc tgtatcggct atttaccctg
    31681 cgatttcagt tctattggct ttcttctttt tgaagcacaa gatttcgaaa aatactgtat
    31741 ttgggattgt cttgattatt ggagggatta ttgcccagac ctataaggtt gaacaggtta
    31801 attccttcta cattgggatt ctctgtgctt tggtttgtgc tattgcatgg ggaagtgaga
    31861 gtgttcttag ctcctttgct atggaaagtg aactgagtga aatcgaagcc ctcttaatcc
    31921 gtcaagtgac ttcgttcttg tcctatcttg tgattgtgct cttctctcat cagtcattta
    31981 ctgcagtagc caatggacaa ttgctaggtc tcatgattgt ttttgcagcc tttgatatga
    32041 tttcctactt ggcttattat atcgctatca atcgcttgca accagccaag gctacaggct
    32101 tgaacgtgag ctatgtagta tggacggtct tgtttgcagt tgtttttttg ggtgcaccgc
    32161 tagatatgct gaccattatg acgtcacttg tcgtcattgc tggagtttat attattatta
    32221 aagaataaag gagattcgtg tgaaagccat tatcttagca gcgggattgg gaactcgctt
    32281 gcgtcctatg actgaaaata cccctaaagc cttggttcag gttaatcaaa aacctttgat
    32341 tgaataccaa attgagtttc tcaaagaaaa aggaatcaat gacatcatca tcattgttgg
    32401 ttatcttaaa gaacaattcg attacttaaa agaaaaatac ggtgttcgcc tcgttttcaa
    32461 tgataaatac gctgactaca ataactttta ctctctctat cttgtaaaag aagaattggc
    32521 caacagctat gttattgatg ctgacaatta tctctttaaa aatatgttcc gcaatgattt
    32581 gacacgttcg acttatttta gtgtttatcg tgaagattgt accaacgaat ggttcttggt
    32641 ctatggagat gactacaagg ttcaagacat tattgttgat agcaaggcag gtcgcatcct
    32701 tagtggtgta tccttctggg atgctccaac tgcagaaaag attgtcagct ttatcgacaa
    32761 ggcttatgca agtggtgaat ttgttgatct ctattgggac aatatggtta aggataatat
    32821 caaagagcta gatgtctatg ttgaagaatt agaaggcaat agcatttatg agatcgatag
    32881 tgtccaagac tatcgtaaat tagaagaaat tcttaaaaac gaaaattaaa gattccaaca
    32941 tctgacaaaa tagtcggatg ttttttgatt ttttacgaac ttttacgaat agatagatga
    33001 gtagaaaaag aaatggagtt atttatgaaa atcacaaact atgaaatcta taagttaaaa
    33061 aaatcaggtt tgaccaatca acagattttg aaagtgctag aatacggtga aaatgttgat
    33121 caggagcttt tgttgggtga tattgcagat atatcaggtt gccgtaatcc agccgttttt
    33181 atggaacgtt attttcagat agacgatgcg catttgtcga aagagtttca aaaatttcca
    33241 tctttctcta ttttagatga ctgttatcct tgggatttga gtgaaatata tgatgcgcct
    33301 gtacttttat tttacaaggg aaatcttgac ctcctgaaat tcccgaaggt agcggtcgtg
    33361 ggcagtcgtg cttgtagcaa acagggagct aagtcagttg aaaaagtcat tcaaggcttg
    33421 gaaaatgaac tggttattgt cagtggtctg gccaagggca ttgacacagc agctcatatg
    33481 gcagctcttc agaatggcgg aaaaaccatt gcagtgattg gaacaggact ggatgtgttt
    33541 tatcctaaag ccaataaacg cttgcaagac tacatcggca atgaccatct ggttctaagt
    33601 gaatatggac ctggcgaaca acctctgaaa tttcattttc ctgcccgtaa tcgcatcatt
    33661 gctggacttt gtcgtggtgt gattgtagca gaggctaaga tgcgttcagg tagtctcatt
    33721 acgtgtgagc gagcaatgga agaaggacgc gatgtctttg ctattcctgg tagcatttta
    33781 gatggactat cagacggttg ccatcatttg attcaagaag gagcaaaatt gctcaccagt
    33841 gggcaagatg ttcttgcgga atttgaattt taaaaatgac ctaagctaga attctaagaa
    33901 aaaatcaatt ttaagagaaa atgaacccaa catttccata ataaaacgca tattagcaag
    33961 tttttaacac ttgctaatat gcgttttttc taagtggatt agtagagtag aggatttttc
    34021 tcatataata ctcttcgaaa atctcttcaa actacgtcag cttccatctg caacctcaaa
    34081 acagtatttt gagctgagtt cgtcagtctt atctacaacc tcaaagcagt gctttgagca
    34141 acctgtggct agcttcctag tttgcgcttt gattttcgtt gagtataagg gaaagtatag
    34201 tgaattgaaa taagatgtga acaactctat caggaaagtc aaattaattt atagaaatat
    34261 tttagcagcc aaggtgcact gttatagatt caatacacta taatttagtg taattgagaa
    34321 aggagaaatg attgtgattg atgttggcta ggttatgttc aatgattcct accatctcaa
    34381 atcttgttag taaggaaaaa taaattcttc aaaagtagag attacaaggc ttgtttaaga
    34441 aagaattcaa agaccttgac aaataaaaat aaaatggtta ttataaaaaa tggtctgaaa
    34501 tagatgatga tacttttcga aaatcttttt acgtcagctc agctttgcct tgctgtgttt
    34561 tgagcaagct acggttagct tccgagtttg attttcattt actagaaatg aaactgatga
    34621 gagatatcag tagacatttg agtcaggata ttatggaaaa tgataaaaag agctcgtgag
    34681 attggcatat cagactacta aagtattgag tttgttagga ttttagcgac tagttagctg
    34741 ggaaaggaag atatttgtga caaataataa actgtattcg ttgatagaat ttagaaataa
    34801 aatatatgaa gaattagaac tttccagaag tgatttagcg attttactat gtgccatgct
    34861 tatcgcctct atcggattaa atatggattc gactcccgtg attattggag ccatgttaat
    34921 ctctcctttg atgacaccta ttctgggagt ggggctctct ctagctatat ttgattttaa
    34981 attgttaaga aaatctttta aaatattagc tattcaaatt cttgccagtt taatagcttc
    35041 aacactttat ttttatcttt ctcccatttc gtatgctagt tcggaaattg ttgctagaac
    35101 ctctccgact atttgggatg ttctcattgc ttttgtagga gggatagcag gtattattgg
    35161 tgctaggaaa aaagagacca ataatattgt tcctggtgtt gctattgcaa ccgccttgat
    35221 gcctcctctt tgtacagtag gttatgctat tgcttctgct aatctaaaat ttatcatagg
    35281 ctcctcttac ctattcctca tcaattgtag ctttattgtc attgcgactt atataggtgt
    35341 taggttgatg atggttaaga aacattattt taaagataat gaagaagact ctaaaatgcg
    35401 taggattttg cttctagttg ctgttttgct gatgattccg agtttcatct ctgcaacgac
    35461 tttagtgaga gaaacgttga aaaaagagtc ccttaagaaa tttatatcag agcagtttca
    35521 ggggtataat attttgaaaa aaacctattc taaaaagact cataccctaa agctaaccat
    35581 ttcaggaaat tatttgacag aagaagaact cgatatgatt tcaagtaaga gaggtgacta
    35641 tggtttaagt gatgtttctg ttcaagtttc acaattgtct gattcagaac aacttagtaa
    35701 ggaagagctg gtggagcatt tcttccagta tatcaaggat aaggaagcaa aagaaaagga
    35761 gaaagccaat aaatttgata cagagtctga ggagcaataa tttcttgaga atagctggtt
    35821 tctctcgtga gtcttctatg tatatcaaag gaagactgag gttttaagta taaaactttt
    35881 cttctattat agtagctaag tcttgacagg gttcaaaaaa tggtttacac tttataaagt
    35941 ttattacttt gaaaaggtgt gatactgtgg ctacggcaac aaaaaagaaa aaatcaacag
    36001 ttaaaaaaaa tctagtcatc gtggagtcgc ctgctaaggc caagacgatt gaaaaatatc
    36061 taggcagaaa ctacaaggtt ttagccagtg tcgggcatat ccgtgatttg aagaaatcca
    36121 gtatgtccgt cgatattgaa aataattatg aaccgcaata tattaatatc cgaggaaaag
    36181 gccctcttat caatgacttg aaaaaagaag ctaaaaaagc taataaagtt tttctcgcga
    36241 gtgacccgga ccgtgaagga gaagcgattt cttggcattt ggcccatatt ctcaacttgg
    36301 atgaaaatga tgccaaccgt gtggtcttca atgaaatcac caaggatgca gtcaaaaatg
    36361 cttttaaaga acctcgtaag atcgatatgg acttggtcga tgcccaacaa gctcgtcgga
    36421 tcttggatcg cttggtaggg tattcgattt cgcctatttt gtggaagaag gtcaagaagg
    36481 gcttgtcagc aggtcgcgtt cagtccattg cccttaaact catcattgac cgtgaaaatg
    36541 aaatcaatgc cttccagcca gaagaatact ggacagttga tgctgtcttt aaaaagggaa
    36601 ccaaacaatt tcatgcttcc ttctatggag tagatggtaa aaagatgaaa ctgaccagca
    36661 atgacgaagt caaggaagtc ttgtctcgtc tgactagtaa agacttttca gtagatcagg
    36721 tggataagaa agagcgcaag cgcaatgctc ctttacccta taccacttca tctatgcaga
    36781 tggatgctgc caataaaatc aatttccgta ctcgaaaaac catgatggtt gcccaacagc
    36841 tctatgaagg aattaatatc ggttctggtg ttcaaggttt gattacctat atgcgtaccg
    36901 attcgactcg tatcagtcct gtagcgcaaa atgaggcggc aagcttcatt acggatcgtt
    36961 ttggtagtaa gtattctaag cacggtagca aggtcaaaaa cgcatcaggt gctcaggatg
    37021 cccatgaggc tattcgtccg tcaagtgtct ttaatacacc agaaagcatc gctaagtatc
    37081 tggacaagga tcagctcaag ctatataccc ttatctggaa tcgttttgtg gctagccaga
    37141 tgacagcggc cgtttttgat accatggctg ttaaattgtc tcaaaaaggg gttcaatttg
    37201 ctgccaatgg tagtcaggtt aagtttgatg gttatcttgc catttataat gattctgaca
    37261 agaataagat gttaccggac atggttgttg gagatgtggt caaacaggtc aatagcaaac
    37321 cagagcaaca tttcacccaa ccgcctgccc gttattctga agcaacactg attaaaacct
    37381 tagaggaaaa tggggttgga cgtccatcaa cctacgcgcc aaccattgaa accattcaga
    37441 aacgttatta tgttcgcctg gcagccaaac gttttgaacc gacagagttg ggagaaattg
    37501 tcaataagct catcgttgaa tatttcccag atatcgtaaa cgtgaccttc acagctgaaa
    37561 tggaaggtaa actggatgat gtcgaagttg gaaaagagca gtggcgacgg gtcattgatg
    37621 ccttttacaa accattctct aaagaagttg ccaaggctga agaagaaatg gaaaaaatcc
    37681 agattaagga tgaaccagct ggatttgact gtgaagtgtg tggcagtcca atggtcatta
    37741 aacttggtcg ttttggtaaa ttctacgctt gtagcaattt cccagattgc cgtcataccc
    37801 aagcaatcgt gaaagagatt ggtgttgagt gtccaagctg tcatcaggga caaattattg
    37861 agcgaaaaac caagcgtaat cgcctattct atggttgcaa tcgctatcca gaatgtgaat
    37921 ttacctcttg ggacaagcct gttggtcgtg actgtccaaa atgtggcaac ttcctcatgg
    37981 agaaaaaagt ccgtggtggt ggcaagcagg ttgtttgtag caaaggcgac tacgaggaag
    38041 aaaagattaa ataagaggag gggcccgaaa gtaaatttca ggctcctttt ggttagaact
    38101 tgacaaaata catcatttta tgcgaaacta gaagaagatt attttatact caatgagaat
    38161 caaagagcaa attagaaagc tagtcgcagg ttgctcaaag cactactttg aggttgaaga
    38221 tggaagctga cctggtttga agagattttc gaagagtatt aactaggaga agttatgcgt
    38281 cttatctatc taattattgg ttttttatcg ctggccttgg ctattgttgg ggttgtttta
    38341 cccttgttgc cgacaacgcc tttccttttg ttgtctattg cttgtttctc cagaagttcc
    38401 aagcgtttcg aagactggct ttatcatacc aagctctatc aaacatatgt agctgatttt
    38461 cgcgagacta agtctattac gcgtgaacga aagaaaaaaa tcatcgtctc tatctacgtc
    38521 ttgatgggaa tttctattta ttttgcacct cttttaccag tcaaaatcgg tctgggtgct
    38581 ttgaccatct ttatcactta ttatctcttc aaggtcattc cagacaaaga atagttaaaa
    38641 cagtagttat ttgccttgat aaaattgaaa gcatattcat aacaatatga tataataaat
    38701 ttgaagtaat attcaaggag aatcaaatga tttacgaatt ttgtgctgaa aatgtgactt
    38761 tacttgaaaa agcgatgcag gctggagctc gtcggattga actctgtgat aatctagcag
    38821 ttggtgggac aacacccagc tatggagtga ctaaggcagc ggttgaactg gcagctaact
    38881 acgatacaac catcatgacc atgattcggc cacgtggtgg tgactttgtc tataatgacc
    38941 tagaaattgc tatcatgcta gaagacattt gtttgactgc tcaggctgga agtcaagggg
    39001 ttgtatttgg agctttaact gctgataaaa agttggataa gcctaatctg gaaaagttaa
    39061 ttgctgcatc aaaaggaatg gaaattgtct ttcacatggc ctttgatgaa ctaagtgatg
    39121 aagatcaacc ggaagctatt gactggctca gtcaagccgg tgtcactcgt atcctaactc
    39181 gtgctggtgt gtctggcgac tccttagaaa aacgttttgt tcactatcac agaattttgg
    39241 agtacgctaa aggtaaaatt gaaattctac caggtggggg gattgacctt gacaaccgtc
    39301 aaacctttat tgaccaggtg ggggtaacac aattgcatgg cactaaggtt gttttttaaa
    39361 aaatagaaag gaactgctag ccttgggtag cagttttcac ttatgtttga aatttttaaa
    39421 tcctatcaat ttaatcaaga aaaggctcat gattatggtt ttatagaaaa tagcgaagtc
    39481 tggacatata gttgccagat tttgcaaggt gactttgtca tgactgtgtc catcactgct
    39541 gataatgtga actttcaagt ctttgaccaa gagactggtg acctctatcc tcacgttcat
    39601 atggaaagca tgaggggaag ttttgtcgga aatgtccgtg aggcttgtct ggagattctt
    39661 taccagattc ggaaggcttg ttttgatgtg caagatttta tctgtcatca gactaagcgt
    39721 atcatgactc aagttcagga aaagtatgga aaccagttgg agtatctgtg ggaaaaatcg
    39781 cctgatacag ctgtattgcg ccatgaaggc aatcaaaagt ggtatgccgt cttgatgaaa
    39841 atctcttgga ataagctgga aaagggcaga gaaggacaag tggaagcagt caacctcaag
    39901 catgaccaag tagctaattt gctttcacaa aaggggattt atccagcctt ccatatgagc
    39961 aagcgctact ggattagtgt gtcccttgat gatactttat cagatgaaga agtactggaa
    40021 ttgatagaaa aaagttggaa cttaacctct aaaaaatgaa atattttaat aattttcatg
    40081 aactttcaat tagctaaata ttctttactg aagaaatttt cagaaaatat aggatttacc
    40141 acaccagagg aatgtggtgc catcttcaaa tacctgattg agaatgtcaa gacggatcgt
    40201 cagatcatct attcgcctca ctgtcatgat gacctcggaa tggcagtggc aaatagcctt
    40261 gctgctgtca agaatggtgc aggacgtgtt gaagggacta tcaatggtat tagggagcga
    40321 gctgaaaatg ctgctttgga agaaattgca gtggctctca atattcgcca agattactac
    40381 caagtagaaa ccagtattgt cctaaatgag accatcaata cgtcagaaat ggtttctcgc
    40441 ttctctggta ttccagttcc taaaaacaaa gccgtcgttg gtggcaatac cttctcccac
    40501 gaatctggta ttcaccaaga tggagtcctt aaaaatcctc tcacttatga gatcatcaca
    40561 cctgaattgg ttggtgttaa gattccgctt ggaaaattat ctggtcgcca tgcttttgtt
    40621 gagaaactga gagaattggc cctagatttt acagaagagg atatcaaacc actctttgct
    40681 aagttcaagg cactggccga taagaagtaa gaaatcacag atgcagatac tcgagctttg
    40741 gtagctggaa ccatggttga aaatccagaa ggcttccact ttgatgattt acaacttcaa
    40801 actcatgcag ataatgacat tgaagcgctc gttagcctag ccaatatgga tggtgagaaa
    40861 gtcgaattta atgcgacagg gcaaggttcc gttgaagcaa tctttaacgc tatcgataag
    40921 ttctttaact aatctgttcg tttggtgtcc tacactatca atgcggtaac agatggaatc
    40981 gatgcccagg atcgggtttt ggtcactgtt gaaaacagag atacagaaac catctttaat
    41041 gcagcagggc ttgattttga tgtgttgaag gcttctgcta ttgcctatat aaacgctaat
    41101 acctttgttc aaaaagagaa tgcaggtgag atgggacgca gtgtttctta ccacgatatg
    41161 cctagtgtgt aaaggagaag gctatggcaa agaaaatagt agctctagta ggagacggaa
    41221 ttggcccaga aatcatggag gctggtttag aagttctgga ggctctagct gaaaaaacag
    41281 gttttgacta tgagattgac agacgaccgt tcggaggtgc agatattgat gcagcaggac
    41341 ctcccttacc tgatgaaacc cttaaggcaa gtagggaagc agatgctatc ctactagcag
    41401 ctatcggtag tcctcagtat gatggagcag cggttcgccc tgaacaaggc ctgatggctc
    41461 tccgtaagga actcaatctt tacgctaata ttcgtcctgt aaaaatcttt gacagtctca
    41521 agtatttgtc accactcaaa ccggaacgaa tttctggtgt agactttgtc gtggtgcgtg
    41581 aattgacagg cgagatttac tttggagatc atattcttga agagcgcaaa gcgcgtgata
    41641 tcaacgacta tagctatgag gaagtggagt ggattattcg caaagccttt gaaattgcaa
    41701 gaaatcgcag aaaaatcctt actagtatcg ataagcaaaa tgttctagcg acctcaaaac
    41761 tctggcggaa agtagctgag gaagtcgcac aggatttctc agatgtaacc ttggaacacc
    41821 agctggtaga ctcagctgct atgcttatga ttaccaatcc tgctaagttt gatgttattg
    41881 taacggagaa tctttttgga gatattttat ctgatgaatc aagcgtctta tctggtacac
    41941 ttggggttat gccatcagcc agtcattctg aaaatggacc aagtctctat gaacctattt
    42001 acggttcagc acctgatatt gcaggtcaag gaattgccaa tcctatttcc atgattttat
    42061 cagttgccat gatgttgaga gatagtttcg gaggttatga ggatgcagag cgtatcaaac
    42121 gtgctgttga gacaagtctg gcggcaggaa ttttaacgag agatatagga ggtcaggctt
    42181 caacaaagga aatgacggaa gctattattg caaggttatg aagttagacg aaaaaattac
    42241 tctagtcctt ttgatttgga atgtcatcat tttcttgatt tatggcattg acaaattcaa
    42301 ggcaagaaga agagcttggc gcatccaaga gaaaatctta ctcattttag cccttacttg
    42361 tggtggtttt ggggcctggt tagctggaat cacttttcac cacaagactc gaaaatggta
    42421 ctttaaaaca gtttggtttc ttgggatggt gaccacacta gtagccttat attttatttg
    42481 gaggtaatgg atggcagggt cttcgaggga atacgctgct tgggctctag cggactatgg
    42541 ttttaaggtc gtgattgcag gatctttcgg tgacattcat tacaataatg gcatgttgcc
    42601 aatcgttcag cctagagagg ttagagaaaa gctagcccaa ctcaaaccga ccgaccaggt
    42661 aactgtagac ttggaacaac agaaaattat ctcaccagtt gaagaattca ccttcgagat
    42721 agatagcgag tggaaacata aactcctaaa tagtttggat gatatcggta ttaccttgca
    42781 gtatgaagag ttgattgctg cttatgaaaa acaacgacca gcctactggc aggattagaa
    42841 aaaatagaaa aggaaatata gtaaactgaa ataagatgta aacaaatgaa ttggagctta
    42901 acatccattt ccagcaattt tttagaaact acagtggact attctggatt caacacatta
    42961 taaaattatg acaaaacaca ttcacaagaa ggctacgaca ttttaaaagg tgagggcgga
    43021 tgtatcgttt gccctactaa agttggttac attatcatga ccagcgataa ggcaggactt
    43081 gagcgtaagt ttgcagccaa agaacgtaag cgtaacaaac caggtgttgt tctctgcggt
    43141 agcatggatg aactttgcgc tttagcgcaa ctcaacccag aaattgaagc attctactaa
    43201 aaacattggg atgaagatat tcttcttggt tgtatccttc cttggaaacc agaagccttt
    43261 gaaaaattca aagcatacgg ggatggccgt gaagaactta tgactgatgt acgtggtact
    43321 agctgttttg ttatcaagtt tggaaaagca gatgaacaat tggctgccaa gctttgggaa
    43381 gaaggtaaaa tggtctacgc ctcatctact tcaatgataa aacgattgaa actcgctatg
    43441 agagcaaggt gtaatggtgt ctatggtcga taagaacggc aaactcatcc cagaacaagg
    43501 aggagcacgt tcaacttcac cagctccagt tgtgatccgt aaagggcttg acattgataa
    43561 aatcatgatg cacctgtcag atacttttaa ctcatgggac taccgtcagg ttgagtatta
    43621 ttaggataga gaagaagtct agtgttaagg acattacagc tcataatact aggctttttt
    43681 atagaaattc ttttcttttt ctataggata tggtattcta tgtagaaaat atatgttaat
    43741 aagtaatgcc aatatttaaa catcattagt aaaaggagtt agattgatga ataaaagaaa
    43801 agttagttta gaagattttt ataaatggta tagtctaaat aaagaaaagt tattaaataa
    43861 ggcaactgtt ggtgaaaagt ttaatgataa attaaaagaa gagtttctcc aggaatggcc
    43921 tttggatagg attttaacaa tgtcaatcga tgaatatgta ataggaaagg gacagcaaaa
    43981 taagtcttta tgctacgctc ttgagaaggg aaaatacaaa aatctatttc ttggaatttc
    44041 tggtggctca gcttcaaaat ttggtattta ttggaataaa aaaacaaaca aatataaaga
    44101 tcaagctaat aatgagattt cagagttgga tcagcgattt tcaaaattaa aatcagattt
    44161 gtatgaaatt atcaaagaag gtattcgttt taactttgaa aatcctattt ttgatatgaa
    44221 aagatcaaca aatgaattta ttggtcgttc tgctatggtg acaaaattac tttgtatcta
    44281 ttctggggga gatcctttct ttggtgtaaa tattaatagt cagaaagaat tttggaacca
    44341 ctttgtttct cagacaaatc aaggtggacc ttatctgcaa aatcataaaa taattgaact
    44401 ggtgtccaaa acttatcctg agttggagcc atcgaaatta ggaactatgc tttttgagta
    44461 ttctaagctt tttatggaaa ataaggaaga caatagtaca atggattcat caaacaattt
    44521 tcgtcatcaa ttaactcaat ctctattaaa gtctccaaac cttatccttc gtggtgctcc
    44581 tggcacagga aagacttatc ttgctaaaga aattgccaaa gaattaacgg atggcaacga
    44641 agaccaaatc ggatttgtac aatttcaccc atcctatgat tatacggatt ttgtagaagg
    44701 tttaagacca gtatcaaatg gggatggagc tattgagttt aggctacagg acggtatttt
    44761 taaagatttt tgtcagaaag caaaagaaac ccaattgatt ggaggacaag ataattttga
    44821 tgaggcttgg gattcttact tagaatatat aaatgttgct gaagaaaaag aatatataac
    44881 aaaaacatct tacttatctg ttaatagtag acaaaatttg tcagtaaatt atgatagtgg
    44941 tgttccagga tggtcactac ctagcaaata tgtttacgag ttgtataaag ataaaaatta
    45001 taataagcaa gaatattaca aaagtggtgg aaaaactgtc ctagaaacat tgagaaagag
    45061 atttggtttg aaagactatg tttccccaac agaaattgat actgataaga aattcgtctt
    45121 catcatcgat gaaatcaatc gtggggagat ttctaagatt tttggcgaac tctttttctc
    45181 tatcgaccct ggctatcgtg gtgaaaaagg aagtgtttct acccaatatg caaatctaca
    45241 cgaaactgat gaaaagttct atatccccga aaatgtttac atcatcggaa ctatgaatga
    45301 tattgatcgt tcagtggata cctttgattt tgctatgcgt cgtcgttttc gttttgttga
    45361 agttactgcc gagggtcaag ttggcatgtt ggataaagag ctgaatatcc atgcagaaga
    45421 agcaaaaatt cgtctaagaa acttgaacgc tgctatcgaa aatgttcagg aattaaacag
    45481 tcattatcat attggaccaa gttattttct taagttgaag gatgtagatt ttgactatga
    45541 attactctgg tctgattata ttaagcctct cctagaagac tacttgcgag gttcttatga
    45601 tgaggttgaa actttggaaa ctttgaaaaa agcatttgaa ctgacaaata atgagcaaaa
    45661 agatcaggca gtagctgatg acaatgaagg tgatgaaaac gatgatgcgg attactgata
    45721 atcaacacaa gattattaaa gaaaaatttg ttgaagaata tcctaaacta agcaatcttc
    45781 ttttagacag aaccttggaa agtctatccc aagatgaacg tattttcatt tttccaaatg
    45841 atttgactca tactcctgat ttggataagg accaaaagat ttttgaaaca gtcaatcaga
    45901 aaatcaagac agggaacgtg attggttttc ttggatatgg tcaggaaaga ttaacgattt
    45961 cctcacgatt ttctgatgag agtaatgacc attttttgca ttatctctta aacaaggttc
    46021 ttcatatcaa tctcactagt ttagatgttg ctttgtctcg tgaagagagg ctttatcaac
    46081 ttttggtgta tctctttccc aagtatctac aagctgctat tcgaaaaggt ctttataagg
    46141 aatatcatcg attttctcat aacgacagta atgttaaggg agtgattgat gtaagaagcc
    46201 atctcaagaa aaatcttcct ttcacgggaa atattgccta cgcaacgaga gagttcacct
    46261 atgataatcc cctcatgcag ttggtccgtc acactattga atacattaag aatcagaaaa
    46321 gcattggtca aggggtacta gataatctct caactagtcg tgaaaacgta tctgaaatcg
    46381 tgcgtgtaac gccctcttat aaactagctg atcgtgctaa gattattcgg ggaaatcaat
    46441 ctaaacctat acgtcatgca tactttcacg agtacagaaa cttacaagaa ctttgtctga
    46501 tgatcctaaa ccaagaaaag cacggtttag ggtatcaaga tcaaaaaatc tatggtattc
    46561 tctttgatgt tgcctggctt tgggaagagt atgtttacac cttgttgcca aaaggttttg
    46621 tacatcccag aaataaggat aagacggatg gaatttcagt attttctgtt gggaaacgaa
    46681 aagtatatcc agatttttat gacagagaac gaaagattgt tctagatgca aaatataaaa
    46741 aactggaatt gactgaaaaa ggaatcaacc gtgaggactt attccagctg atttcctatt
    46801 cttatatttt aaaagctgag aaggctggac tgatttttcc tagtatggag cagtcagtaa
    46861 atagtgaaat aggaaaagta gctggctatg gagctcaatt gaagaagtgg tctattcgaa
    46921 tccctcagaa tgcctcattc tatagtacat tttgtaaaat gatggaaaat tcagaagaaa
    46981 attttaaagc gattattgat gaagaagtgg ggagaaaata atttcccctc tttcttttat
    47041 ttttaacaca agtaaaaccc gaacgatata ttttaattca taaaataatt tgacaaaaac
    47101 tgtactttgg tttataataa attaaggaaa cgtcggaaaa ggcgtagtga tgcaagagca
    47161 taggtaggtc attacaaaag aaacgagaca tcgatatgtt aaatgaattt ccaatttttg
    47221 attacgaaga tattcaattg attccaaata aatgtgtcat taaaagccgt gcagaagcgg
    47281 atacaagtgt cactttagga aatcacacct ttaaactacc tgttgtgcca gcgaatatgc
    47341 agacgatttt ggatgaaaat gtagcagagc aactggctaa aggtggttac ttctacatta
    47401 tgcaccgttt tgatgaggca ggacgcattc cttttattaa acgaatgcac gatcaagggc
    47461 tcattgcttc tatctctgtc ggtgttaagg attatgagta tgatttcgtt agacagctca
    47521 aagctgatgc tcctgagtat atcactattg atattgctca tggtcatgcg gatagcgtga
    47581 tttctatgat tcaacacatc aagaaagaat tgccagatac ctttgtcatt gctggaaatg
    47641 tgggaacacc agaagctgtg cgtgaattgg aaaatgctgg tgcggatgct actaaggtcg
    47701 gaatcggtcc tggtaaggtt tgtatcacca aggttaagac tggttttggt acaggtggtt
    47761 ggcagttggc tgctctacgc tggtgtgcca aggctgcgcg taaaccgatt atcgctgatg
    47821 gaggaattcg tactcacggt gatattgcta agtctatccg cttcggtgct agcatgatca
    47881 tgattggttc cctctttgca ggacacattg aaagtccagg gaaaacgatt gaagtcgatg
    47941 gtgaacagtt caaagaatat tatggttcag cctcacaata tcaaaaaggt gcttacaaaa
    48001 atgtggaagg caaacgtatc ttgcttcctg ctaaaggtca tctgcaagac actttaactg
    48061 agatggaaca agaccttcaa agtgctattt cttatgcagg tggacgtcag gttgctgacc
    48121 ttaaacacgt tgattatgtg atcgtgaaaa actccatctg gaatggggat gcttcccact
    48181 aagacggggt ctttgaccaa aaaacttgtt attagagcaa atttctgtta taataaaaca
    48241 agtttccacc cttagtgtaa tggatatcac gtaagattcc ggttcttgag atgggggttc
    48301 gattccctca gggtggatgt aaacatccta aaaagccttt aatagggctt ttttctttat
    48361 cctgacccaa atttgccgct aaaactgaga atctctgtct aaagaattcc caaatgcttt
    48421 ccagtcccgt tttagagtga ctcagggggc tttttttgat ataataaaaa ggactattat
    48481 cagttagaaa gaggttggta tgaaagaatt acaaactgta ctaaagaatc attttgcaat
    48541 cgaatttgca gacaaaaatt tactggaaac tgcctttact catacgagtt atgccaatga
    48601 gcaccgcctc ttaaaaattt cacacaatga acgcttggaa tttttaggag acgctgttct
    48661 acagttattg atttcagaat atctatataa aaaatatcct aaaaagcctg aaggtgacct
    48721 atcaaaactc cgtgctatga ttgtccgtga ggagagttta gctggttttg cgcgtgattg
    48781 ccagtttaac cagtttatca agttgggtaa aggggaagaa aaatcaggtg gtcgcaatcg
    48841 tgacaccatt cttggtgatg cctttgaagc ctttcttggt gcccttcttt tggataagga
    48901 tgtggccaag gtcaaggaat ttatctatca agtcatcatt cctaaggttg aagcaggcga
    48961 gtttgagatg attacagact ataaaaccca tctccaagag ttgcttcagg tcaatggtga
    49021 tgtggctatt cgttatcagg tgatttctga aacagggcct gctcatgata aggtctttga
    49081 tgtagaagtt cttgttgaag gtaagagcat cggtcaaggc caaggtcgtt ctaagaaatt
    49141 agcagagcag gaagctgcca aaaatgccgt tgagaaggga ctggattcat gtatttaaag
    49201 gaaatcgaaa ttcaggggtt caagtctttt gctgataaga ccaaggtcgt ttttgaccaa
    49261 ggtgtgacgg cagttgttgg acccaatgga tctggaaagt ccaatattac agaaagtctg
    49321 cgttgggctt tgggggagtc tagtgtcaag agtctccgtg ggggcaagat gccggatgtc
    49381 atctttgctg gaaccgaaag tcgcaaaccg ctcaattatg cttctgtagt tgtgactctg
    49441 gataatcatg acggatttat caaggatgca ggtcaagaaa tcagggtgga acgccatatc
    49501 tatcgtagtg gagatagcga atacaagatt gacggcaaga aagtccgtct gcgtgatatt
    49561 catgacctct tcttggatac tggattggga cgagattcct tctctattat ttcccaaggg
    49621 aaggttgagg agatttttaa ttccaagcct gaggaacgac gagctatttt tgaagaagct
    49681 gctggagttt taaaatacaa gactcgcaga aaagaaaccg agagtaaact gcagcaaact
    49741 caggataatc tggaccgctt agaggacatt atctacgagt tggataatca aatcaagcct
    49801 cttgagaagc aagctgagaa tgcccgtaag tttttagact tggaaggaca acgtaaggct
    49861 atttatttag acgttctggt tgctcaaatc aaggaaaata aggctgaact agagtcgaca
    49921 gaagaagagt tgactcaggt tcaagaactc ttgatgagtt attaccaaaa gcgtgaaaaa
    49981 ttagaagaag aaaatcaaac tcttaaaaag caacgccaag atttacaggc tgaaatggcc
    50041 aaagaccaag gcagtttgat ggacttgact agtctgatta gtgatttaga aagaaaatta
    50101 gccctatcga aactggagtc cgagcaagtg gccttgaatc aacaggaggc acaagcccgt
    50161 ttggctgctt tggaggataa gagaaattca ctcagcaaag aaaagtctga taaagaaagc
    50221 tctttagctc tgttagaggg aaatctagtc caaaataatc aaaaactcaa tcgtttagaa
    50281 gctgaattgc tggctttttc agatgatcct gatcagatga ttgagctttt gcgtgaacgc
    50341 tttgtggcgc ttttacaaga agaagcggat gtctcaaacc aactgacccg catcgagaat
    50401 gagttggaaa atagtcgtca gctttctcaa aaacaagcag atcaactaga aaagctgaaa
    50461 gagcaattgg ctatagctaa agagaaggct agtcagcaaa aggaagagct tgaaactgcc
    50521 aaggagcagg ttcagaaatt attggctgac tatcaagcta ttgccaagga gcaagaggag
    50581 caaaaaactt cctatcaagc tcaacaaagt caactctttg accgtctgga tagtcttaaa
    50641 aacaagcagg ctagagctca aagtttggaa aacatcctta gaaatcacag taacttttat
    50701 gcaggtgtaa agagtgttct ccaagaaaaa gaccgccttg gtgggattat tggtgctgtt
    50761 agtgagcatt tgacctttga tgtgcattat caaactgcct tagagattgc acttggatcc
    50821 agcagtcagc atatcatcgt agaagatgaa gagtcggcaa ccaaagctat tgatttcctt
    50881 aaacgaaaca gagccggtcg tgcaaccttc cttccgttga caacaatcaa ggctcgtacg
    50941 atttctagcc agaatcaaga tgttatcgct gtaagcccag gtttccttgg gatggcagat
    51001 gagttggtga cttttgatac tagactggaa gccattttca agaatttgct agctacgacg
    51061 gctatttttg atactgtaga gcatgcgcgt gcagcagctc gccaagttcg ttatcaggtt
    51121 cgtatggtga cattggatgg gacagaatta cgcacgggtg gttcctatgc gggtggtgcc
    51181 aatcgccaaa ataacagtat tttcatcaag ccagaactgg agcaattaca aaaagaaatt
    51241 gctgcagatg aagcaagctt gggttcagaa gaagcggctt tgaagacctt gcaagaccag
    51301 atggctacat tgacagaaag attagaagcc atcaaatctc aaggagaaca ggcacgtatt
    51361 caggagcaag gcttgtccct cgcttatcag caaactagtc agcaagttga agaactggaa
    51421 actctttgga aactccaaga agaggaaaaa gatcgtcttt ctgatggaga ttggcaagca
    51481 gacaaggaaa actgccaaga gcgtcttgct gcaatcgcca gtgacaagca aaatctggaa
    51541 gctgagattg aagagattaa gtctaataaa aatgccatcc aagaacgcta tcaaaacttg
    51601 caggaagagc tagcgcaagc tcgtttgctt aagacagaac tgcaagggca aaaacgttat
    51661 gaagtggctg atattgaacg cttaggcaag gaattggaca atcttgattt tgaacaagag
    51721 gaaatccagc gccttcttca agaaaaggtt gacaatcttg agaaggttga tacagaattg
    51781 ctcagtcaac aggcggaaga atccaaaact cagaaaacga acttccaaca aggtttgatt
    51841 cgcaaacagt ttgagttgga tgatatagaa ggtcagctgg atgatattgc tagtcatttg
    51901 gatcaggctc gccagcagaa tgaggagtgg attcgcaagc aaacacgtgc tgaagccaag
    51961 aaagaaaagg tcagcgagcg cttgcgccat ctacaaagtc aattaacaga ccagtaccag
    52021 attagctata ctgaagctct agaaaaagcg catgaattgg aaaacctcaa tctggcagag
    52081 caagaagtta aggatttaga gaaggctatt cgctcactgg gtcctgtcaa tatagaagct
    52141 attgaccagt acgaagaagt tcgcaaccgt ctggactttc taaatagtca gcgagatgat
    52201 attttgtcag cgaaaaatct gctccttgaa accattacag agatgaatga tgaggtcaag
    52261 gaacgcttta aatcaacctt tgaagctatt cgtgagtcct ttaaagtgac cttcaagcag
    52321 atgtttggcg gaggtcaggc agacttgata ttgactgagg gcgacctttt aacagctggt
    52381 gtggagattt ctgttcaacc tccaggtaag aaaatccagt cgcttaacct catgagtggt
    52441 ggtgaaaaag ctctatcggc tcttgccttg cttttctcca ttattcgtgt caagactatt
    52501 ccatttgtta tcttggatga ggtggaagct gcgctggatg aagctaatgt taaacgtttt
    52561 ggggattacc tcaaccgctt tgacaaggac agccagttca tcgtcgtaac ccaccgtaag
    52621 ggaaccatgg cagcggctga ttctatctat ggagtgacca tgcaagaatc aggtgtctca
    52681 aaaattgttt cagttaagtt aaaagattta gaaagtattg aaggatgaca attaaactag
    52741 tagcaacaga tatggatgga accttcctag atgagaatgg gcgctttgat atggatcgcc
    52801 tcaagtctct cttggcttcc tataaggaaa aagggattta ctttgcggtg gcttcgggtc
    52861 ggggatttct gtctctggaa aaattatttg ctgatgttcg tgatgacatt attttcatcg
    52921 cggaaaatgg cagtttggta gagtatcaag gtcaggactt gtatgaagcg actatgtctc
    52981 gtgactttta tctggcaact tttgaaaagc tgaaaacgtc accttatgta gatatcaata
    53041 aactgctctt gacgggtaag aaggggtcat atgttctaga tacggttgat gagacctatt
    53101 tgaaagtgag tcagcattat aatgaaaata tccaaaaagt agcgagtttg gaagatatca
    53161 cagatgacat tttcaaattt acaaccaact tcacagaaga aacgttagaa gctggtgaag
    53221 cttgggtcaa tgataatgtc cctggtgtca aggctatgac aactggcttt gaatctattg
    53281 atattgttct ggactatgtc gataagggtg tagctattgt tgaattagct aaaaaacttg
    53341 gtatcacaat ggatcaggtc atggcttttg gagacaatct taatgactta catatgatgc
    53401 aggttgtggg acatcctgta gctcctgaaa atgcacgacc tgaaatttta gaattagcaa
    53461 agactgtgat tggtcaccat aaggaacggt cggttatagc ttatatggag ggcttataat
    53521 ggcagatata aaattgattg cattggactt ggacgggacc ttgctgacta ctgataaaag
    53581 gctgacggat cgtaccaagt caaccttgaa agctgcgcgt gatcgtggta tcaaggtcgt
    53641 attgacaact ggtcgtccct taaaagccat ggacttcttt ctccatgagt tagggactga
    53701 cggccatgaa gatgagtata ccattacttt taatggtggt ttggttcaga aaaatacagg
    53761 tgaaatcctt gataaaacag tcttttcata tgctgatgtg gcacgcttgt atgaggaaac
    53821 agagaaatta tcactgcctc ttgatgccat ctcagaagga acagtttatc aaatccaatc
    53881 ggaccaagaa agtctttatg ccaaattcaa tccagctttg acttttgttc cagttgactt
    53941 tgaagacctg tctagtcaaa tgacctacaa caaatgcgtg actgcctttg ctcaagaacc
    54001 cttggatgca gccattcaga agatttctcc agaattgttt gaccaatatg aaatctttaa
    54061 atcacgtgaa atgttgctag aatggtcacc aaagaatgtt cataaagcaa caggtttggc
    54121 aaaactaatc agccatcttg gaatcgacca aagccaagtg atggcttgtg gtgacgaggc
    54181 caatgacctc tctatgattg aatgggcagg tcttggtgtt gctatgcaaa acgctgttcc
    54241 tgaagtaaag gcagccgcaa atgtagtgac gccgatgacc aacgatgagg aagctgtcgc
    54301 ctgggctatc gaagaatatg tgttaaagga gaactaagat atgggattat ttgaccgtct
    54361 attcggaaaa aaagaagaac ctaaaatcga agaagttgta aaagaagctc tggaaaatct
    54421 tgatttgtct gaagatgttg atcctacctt cacagaagtt gaggaagttt ctcaggaaga
    54481 agcagagatg gaaattgttg aacaagctgt gttccaagaa gaggaaatcc aagacacagt
    54541 tgaagaaagt ctggatttag agccagttgt agaagtttct caaaaagaag tagaagaatt
    54601 tccaaactca caagaagtca cagaggaaga gaagcttgag cacgaaggaa ctgtagaaga
    54661 aaataattct gaagtgcttg aaccagaagc tcctcaaaca gaagaaactg ttcaggaaaa
    54721 atatgaccgc agtcttaaga aaactcgcac aggtttcggt gcccgcttga atgccttctt
    54781 tgctaacttc cgctctgttg acgaagaatt tttcgaggaa ctggaagaac tgctgattat
    54841 gagtgatgtt ggtgtccaag tcgcttctaa cttaacggag gaactacgtt acgaagccaa
    54901 gcttgaaaat gccaagaaac ctgatgtact tcgtcgtgtc atcattgaga aattggttga
    54961 gctttatgaa aaggatggta gctacgatga aagcatccac ttccaagata acttgacagt
    55021 tatgctcttt gttggtgtga atggtgttgg gaaaacaact tctatcggaa aactagccca
    55081 ccgctacaaa cgagctggta agaaggtcat gctggttgca gcagatacct tccgtgcggg
    55141 tgcagtagct cagctagctg aatggggccg acgagtagat gttccagtag taactggacc
    55201 tgaaaaagct gatccagcca gcgtggtctt tgatggtatg gaacgtgccg tggctgaagg
    55261 tatcgatatt ctcatgattg atactgctgg tcgtctgcaa aataaggata accttatggc
    55321 tgagttggaa aagattggtc gtattatcaa acgtgttgtg ccagaagcac cacatgaaac
    55381 cttcttggca cttgatgcat caacaggtca aaatgcccta gtacaggcca aagaattttc
    55441 gaaaatcaca cctttaaccg gaattgtttt gactaagatt gatggaactg ctcgaggagg
    55501 tgtggttcta gccattcgtg aagaactcaa tattcctgta aaattgattg gttttggtga
    55561 aaaaatcgat gatattggag agtttaactc agaaaacttt atgaaaggtc tcttggaagg
    55621 tttaatctaa tcagaagcaa aaatcctgca aggcataaac ttgcaggaaa tttttttatt
    55681 ctaagcgacc atcttgacga taggcgatat ctggttgcca agtccatttg gcaccgaatt
    55741 tttcaagtag gtcaaagctg gcttgaggtc ccatgcttcc agctttatag tcatgaagtg
    55801 gggcaccatt ttcagcccag agcttttcaa tacgatcaat caacttccat gacgcaccaa
    55861 cttcatccca gtggctaaag ttagttgagt tgttatttag gacatcataa atcaattttt
    55921 cgtatggttc tggagaagca ccagttgcag tcgcgtctgt acgataatca agtgagttag
    55981 gagccaagtt aaattcttct cctacttgct tcccatttag gctaagagag aagccttctg
    56041 ttggttgaat atagatggtc aaaatatttg gagcaagtgg ttctccaaag atagaatcca
    56101 tttgtttaaa gacgatgttg acatgagttc ctttttcagt cagtcgttta cctgtacgga
    56161 aaaagaaagg aacaccacgg aatcgatcgc tgtctacaaa gaaggcacca gatgtaaagg
    56221 tttcagttgt tgattctgga ttcacatttg gctcgctacg ataagagatg tattttatgc
    56281 catcaatctt accagagcgg tattgcccac ggataaagtg ttctttgagt tcttcatcag
    56341 ttggatgata gaggttttta aagaccttaa tcttttcagc acgaatctcg tcttttgtga
    56401 agcttgctgg tttgtccatg gcgagaagcg aaagaagttg tagagtgtgg ttttggacca
    56461 tgtcacggag ggcaccggat tggtcatagt agccaccacg ttcttctaca cccaagcgct
    56521 ccgcaaaggt aatttgaaca ttgtcgataa aatccttgtt ccaaacgttt tcaaaaatca
    56581 agtttgcaaa gcgaactgca aagatgcttt ggatcatttc cttaccaaga taatggtcga
    56641 tacggaaaat ttgttcttcg tcaaatgttg ctaggagttc gtcattcaac ttgcttgcag
    56701 ttgcgtaatc tgtaccaaat ggtttttcaa cgatcaagcg ctcaaaacct ttgccatcga
    56761 caatgttttc agatttgagg tgtttggcaa tggttccaaa gaactgaggt gccatagaca
    56821 agaagaagag cttattgtgt tcagcttggt atttttcatt aagctcagct tgtaattgac
    56881 gcaaagcaat ataatgttcc gaatcattga catcatggct ttgatagtag aagtggctag
    56941 caaattcttg ggcttgctcg gtactatctg ccaaatcaag gatggactcg acaactacag
    57001 attcaaaata ttccttactc caaggtctac gggcagttcc aataacggca aagtgcttgg
    57061 aaagattgcc ggatttatat agtctaaaaa gggaagggta gagcttgcgt ttagccaggt
    57121 ctccactcgc accgaaaatt gtaacaataa ccttagatga catctagcta cctaatttct
    57181 atttttattc ctagtcttgc taggtatgag gaaacctcat ttcataaact taattctaac
    57241 ataattttcc gaaaaatcaa aaattccaat acgaggtaga aaaactgcaa ggaaatcctt
    57301 acagtttgag tttagacgtt taagacctta tctaagaact ctttcagacg agggtgttgt
    57361 gggttatcaa agatttggtc aggtgttccg tcttcaagga actcgccatc tgcagtaaag
    57421 ataacgcggt tggcaacctg acgagcaaat cccatctcat gggttacgat aatcatggtc
    57481 atgccttgct cagccaattc cttcataacg ttaagtacgt ctccaaccat ctcagggtca
    57541 agggcagaag ttggttcatc gaagagcatg atgtctggat tcattgctag gccacgagct
    57601 atggccacac gttgtttttg accacctgat aggctatctg gattggcatt agctttatct
    57661 gctagtccaa ccttttcaag caactccatt cccaatttct cagcttcttc cttagtcatc
    57721 aacttgtgct caataggagc aaaggtgatg ttgtccaata cagacatatg agggaagagg
    57781 ttgaagtgtt ggaataccat gccgatattt tcacggacgt ggtcaacatt ggttgttttt
    57841 tcagttaaat catagccgtt cacagtgatg tgaccgctag tgacttcttc taaaagattg
    57901 aggctacgga ggaaagttga cttaccagaa cctgaaggac cgatgataca aacaacatct
    57961 ccttcataga acttagtcgt aattcctttt aggacttcat tttttccata gtgcttgtgt
    58021 aaatcattta catcaatttt taattttgcc attaacgaat cctcttttct aagcgtttcg
    58081 ctagtctagt caaaagtgtg ataattacaa gatagaagat agcaaggatt gcatacatct
    58141 tgaaactttg gtagttacga gcaatgataa tcttaccagt ttggaagagt tcaaccaaac
    58201 cgatagcaga tacgatagtt gtatctttaa gagcgataac gaattggttg acaaagtttg
    58261 gcaacatcaa tttagttgct tgtggcaaga taatcttacg catggttttt ccataagaga
    58321 tacccaagct tcggctggct tccatttggc caactggaac ggcctgaata ccaccacgaa
    58381 cgatttcagc gatataagca gccgcattga gtgagagggc aattgttcca gctacaaagt
    58441 cgttaattgg gctttgttgg cctgtgatag actcgatgaa gtttggaatt ccccagaaga
    58501 tgaaggctgc aagaatcatc aatggaatac cacgaataac gtcaacgaaa atctcagaga
    58561 tgacgcgaag agatttgtat gggctaacgc taaacatacc gaagataatt ccgatgacaa
    58621 tggcaatagc aaatgagata agagctagag caagagtgat accaagaccg ctaaggagtt
    58681 gtttgtagtt gttttgaagc aagccccaga gcgttgtttc gtcaacagta cttgttgaag
    58741 cagttgaaga ttcgctagct aggtatttgt caagaatctt ttggaattca ccgtttgctt
    58801 taaggtttgc aagtccgttg ttgaacattt caatcagttc tggatttgct ccttttttaa
    58861 cggcaaaggc tgtttcaccg attggagttc cagagattgg agttttcaat ttttgacctt
    58921 ggctgataga atatttgaga acaggttcat catccataac ggcatcaatg gcaccagtgt
    58981 ttaaactgtc atacattgaa gaaccatcag caaaggtttt gattttgtag ccgtatttgc
    59041 tttgattttc tgttaggaag gtttgagaag cagttccgtt tttaacaccg actgtctttc
    59101 cttttagatc ttcataagaa gcaatattac ttgattcttt gacaccaaga atggtattag
    59161 cagtgtagta tgattctgag aagtcaaaag ttgccttacg agcatctgtg acagacatac
    59221 cagcgatgat accatcggct tgaccagctt ggacagcact gatagcagca tcaaaaccag
    59281 ggttggtgat ttcaatttca aaaccttggt ctttagcgat tgccttaatc aattccatat
    59341 caataccagt gtattggttg cttgaatttt ggaaaacaaa aggggcaaaa gaagaatcgc
    59401 tggcaatgat atatttagcc ttaacaggaa tagcttttaa tcctgcgaga gtagttgtag
    59461 ttggcactgc tgaagatgat gaagcagtcc atttcttgat aattttatca agactaccat
    59521 cttttttcat ttcagacaag gcttggttaa attcagtaac caggtgctcg tatttacttc
    59581 cttttttcac accgaaagca aaacttccta cagcttcacc atccatttca atatggaggt
    59641 cttgaccttg gttaatggca tattcgataa caggtttgtc atccatcatg gcatcgatgg
    59701 caccagcact caagctgttg ttcattaaat caccagtgtc aaatgtttta atagtaaagc
    59761 catatttatc tttgattgtt tcaaggaaac gttgagcggc agttccgttt ttaacaccaa
    59821 cggttttgcc agttaattgg tcgtacttgc taattttgtg tgactttgta gtagcaatga
    59881 caacttttgt atcatagtaa gtatcagaca tggtgaagac tttttcacgt tctttagtct
    59941 ttgtcatccc tgccatgata gcgtcggctt gcccagcttg aaccgcattg actgctgcgt
    60001 caaatccagg ataggacatc tgaatgttcc agcctttaat ctcagcgact ttgttaataa
    60061 tgtcaacatc aattccttta taagtttgat ctgaatcttt aaactcaaaa ggtgcatagg
    60121 cggtatcaga aacaatctta atcgtttctg ctttggcaat acctaatgag aaaattggga
    60181 ataaaattag caaaaatgct agaaattttt tcttcatttt tagtctcctt ttccgaatat
    60241 tctctcatta tatcagaaaa agtaaaaaaa ggcaaatttt tatatttttc gtgtcagaaa
    60301 atataacatt gatttttcct tttctttctt gaattgctat taaaaagtgc tataataata
    60361 gtatatatag aagaaaagag gacggggata tgaagaacaa aagaatattt aaagacttcc
    60421 aagcttcaaa aatgagttta aacatttaca caagcccctt gttagccttt gtttttgtct
    60481 tcataggaga gtttgtggct tttactttgt atggtattgg cttgttagct ctcatcggac
    60541 ttgctagaaa ttttggagag gctggtcaaa atcttgcaac ctacttgcag accttgcatc
    60601 agagcttgac ggataaaaca agtgactttc gtttaatttt aggattactg gcctttggtt
    60661 ttattcttaa cactgtgttc agatggacaa gaaaagttga gaaaagacct attcgaacct
    60721 tgggatttta tagagagaat ttcctcagca atcttctgaa aggatttagt ctaggcttgg
    60781 cactttttct tctgaccttg ttaggtttag tggtcttagg tcaatatcgt ttggaatcca
    60841 ttcacttgaa tccttattct cttgcctttg tcgtctttac tatcccattt tggattttac
    60901 aggggacagc agaagaagtg gtggcccgtg cttggctact tcctcaattg gcctcaagaa
    60961 ccaatctaaa actagctatt cttatatcta gcctgttctt taccctgctt catatgggca
    61021 attctggcct cacccctcta tctctagtaa atctcttttt attcggagtt gccatggctc
    61081 tttatcttct caaaactgat acagtttggg gtgttgcagg tattcatggt gcttggaatt
    61141 ttgctcaggg taatctcttt gggattttag ttagtggtca accgtcagga acgtctctga
    61201 tgaccttttt accacaaggc aatcaagatt ggctatcagg tggttctttt ggcatagaag
    61261 gttccattat gacaagtctg gtattactac tgctgattgt ctatcttgct aataaattaa
    61321 agaaagaaaa tgaaaggatg tgacttcggt ccgtcctttt cttcgtgaaa atactataag
    61381 tatgctaaaa taggaatagc acatggagag aggattctta tgatcaatca cattacagat
    61441 aatcaattta aactagtatc aaaatatcaa ccatcaggag atcaacccca agctatcgag
    61501 cagttggtgg ataacattga ggggggagaa aaagctcaga ttctgatggg ggcgactgga
    61561 acagggaaga cctatactat gagtcaggtc atttctaaag tcaataaacc aactctggtt
    61621 attgcccaca ataaaactct ggctggtcag ctctatgggg agtttaagga atttttccct
    61681 gaaaatgcag ttgagtattt cgtatcctac tatgattatt accagccaga ggcctatgtc
    61741 ccttctagcg atacctatat tgagaaggat agttctgtca atgacgagat tgacaagctt
    61801 cgccactcag ctacctcagc ccttttggag cgtaatgatg ttattgtcgt ggcctcagtc
    61861 tcttgtatct atggtttggg ttcgcccaag gaatacgctg atagtgtcgt tagtctccgt
    61921 cctggtctag agatttctcg tgataaactc ttgaatgact tggtcgatat tcagtttgaa
    61981 cgtaatgata ttgatttcca acgcggaaga tttcgcgttc gtggggatgt ggtagagatt
    62041 ttcccagctt cccgagatga acatgccttt cgagtagaat tttttggaga cgaaattgac
    62101 cgtatccgtg aggttgaggc tctgacaggt caggtattgg gagaggtgga tcatttggcg
    62161 attttcccag ctactcactt tgtgaccaat gacgaccata tggaagttgc cgttgccaag
    62221 attcaagcag aattagaaga gcagttagct gtctttgaaa aggaaggtaa actgcttgaa
    62281 gcccagcgtt tgaaacagcg gacagagtat gatatcgaaa tgttgcgtga gatgggctat
    62341 accaatgggg ttgaaaatta ttctcgccac atggatggac ggagcgaagg agagcctcct
    62401 tatacgcttc tcgacttctt cccagatgat ttcttgatta tgattgacga gagtcatatg
    62461 accatagggc aaatcaaggg catgtacaat ggagaccgtt cgcgtaagga aatactagta
    62521 aattatggtt tccgtttacc gtctgccttg gacaatcgtc ctctccgtcg tgaggagttt
    62581 gaaagccatg tgcatcagat tgtttacgta tcagcgacac ctggtgacta tgaaaatgaa
    62641 cagactgaga cggtgattga gcaaatcatt cgaccgacag ggcttttgga tcctgaggtg
    62701 gaagtccgtc cgactatggg acagattgat gatctcttag gtgaaatcaa tgcccgtgtt
    62761 gaaaagaatg aacgaacctt tatcaccacc ttgaccaaga aaatggcaga ggacttgacc
    62821 gactatttca aggaaatggg catcaaggtc aagtacatgc actcggatat caagaccttg
    62881 gaacggacgg agattatccg tgatctccgc ttgggtgtct ttgatgtctt ggttggaatc
    62941 aatctccttc gtgaagggat tgacgttcct gaggttagct tggttgccat tctcgatgct
    63001 gacaaggaag gtttccttcg taacgagcgt ggactcatcc agaccattgg gcgtgccgcc
    63061 cgtaacagtg aaggtcatgt catcatgtat gcagatacgg ttacccagtc tatgcaacgt
    63121 gctatcgatg aaactgcccg ccgtcggaaa attcagatgg cctataatga agagcatggt
    63181 atcgtaccac agacaatcaa gaaagaaatc cgtgacctga ttgccgtgac caaggcagtt
    63241 gctaaggaag aggacaagga agtcgacatc aatagcctca acaaacaaga gcgcaaagaa
    63301 ctcgtcaaga aactagaaaa acaaatgcaa gaagccgtcg aagtgcttga ctttgaactg
    63361 gcggctcaga tccgtgatat gatgttggaa gtcaaggcct tggattaggg gaatagtatg
    63421 atttatttaa gaaagttaaa gaaagaagat ttgatgtctt tatgggaaat ggcttattca
    63481 cagcttaatc cagtttggaa acagtatgat gctccctatt atgatgatta tcagtatttt
    63541 tcaaatttta aagaattcga actacaaaaa tcagaatcca ttttaagcaa ctcaaatcgc
    63601 cttggtattt ttgttgatga taaactagtt gggactgttt cgcgttattg ggtatgtaaa
    63661 gaaacaagat ggatggaatt gggaattggt atttatgata aaaaattctg gaacactggt
    63721 attgggaaag ttgctatgtt gcagtggata gataggacgt ttcaggatta cttggagttg
    63781 gagcatctgg gtttgacaac ttggtcaggg aatcttggta tgatgaaact tgctgaaaaa
    63841 ttaagaatga aaaaagaagc tcatattcca aaagttcgtt attatcaagg taaatatttt
    63901 gatagtatta aatatggtat tttgagagaa gactgggaga aaataaatga cggttattat
    63961 caaatcaatg gaaactcctg aagagataga aggtaaatcc ctcgttcact ggcaaacgtg
    64021 gagagaggct tatgatgacc ttttgcctgc agaatttcag gagacaatga cattagaaag
    64081 atgtcgattc tttagtcaaa agtatccaga aaatacattg attgcgatag gtgatctgaa
    64141 ggtagttggt tttatcagtt atggtaactt tcgtgatgat actattcaag ctggtggaat
    64201 tattgcttta tatgttttaa aagactatta cggaaaaggc atagcacaaa agttaatgaa
    64261 agcagctttg actgctctta atcatttttc tgaaattttc ttatgggtat tgaaagataa
    64321 caagcgcgcc attgctttct atcaaaaaat gggttttact tttgatggac aagaaaaaat
    64381 acttgaactt ggaaagccta taaaggaaaa acgaatggtg ttctattcta aataatttta
    64441 aaaaagtaaa agataatatc tctggtagta agtctgaaaa tttaataaat tagaaagcga
    64501 gtaaatttat gtcccgttcc caattaacaa ttttaacaaa tatctgtctg attgaagacc
    64561 tcgaaacgca gcgcgtggtg atgcagtatc gcgcccctga aaacaatcgc tggtctggtt
    64621 atgcctttcc tggaggtcat gtagaaaatg atgaggcttt tgcggagtct gtcattcgtg
    64681 aaatctacga agaaacaggg ttgactatcc aaaatcctca acttgtcggc attaaaaatt
    64741 ggccactaga tacaggtggg cgctatattg tcatttgtta taaggcgact gagttctctg
    64801 gtacccttca atcttcagaa gagggagaag tttcttgggt gcaaaaagac cagattccaa
    64861 acttaaatct ggcctatgat atgctaccat tgatggaaat gatggaagct cccgacaagt
    64921 cagagttttt ctaccctcgc cgtacagaag acgattggga aaagaaaatc ttctagtctt
    64981 ttactaaata acctagctga tccaaggcct cctcgatata gtggaggtct tgttgtgttt
    65041 cggcttcgac taggtggtaa tggatgccat ctgtcaattc agatagaggt ctaaagtcag
    65101 aatgctcgac ttgttctaga aaatgttgca catctctgcg acaagacagt ttaagcaagg
    65161 tttcaatttc tccataaacg ggatggtcaa tcaaggtatt ctggacatgc ccaccattat
    65221 cgacaatagc aaggagttcc tgaccgattt cttcaacttc atgtttcact ttgaaaagtt
    65281 tgtgaacata aggattggta tcaatttgct tatagatgta gccacgattg gtggatagga
    65341 taggagcgcc atcggctctc aaaattgcaa tgtcctgcac aatgacctga cgtgtgacat
    65401 gaaaatgttc cgccaaactt tggccattaa gggctttagg agcttctttc aagagttgga
    65461 gcagggcttg tttgcgatct tttgtcatag cttttccttt taacggcgtt ttcgaagcac
    65521 tttatagaca gctagtgcta atgtatagtc taccatacta tggataattg tgccaaatcc
    65581 aactagtaca aatagaacat agaacatgtt ttctacattg gtacccgaag ttgcgtaaaa
    65641 aaggacacag gctaatactt cagcaagggc atgaacaaca gccaaaacaa aattgaaaac
    65701 ccaggaagat tttggtttat ctagggtatc ggggaatttt tgtaggtaaa gagctcctaa
    65761 agcaccaaaa gatatatggg aaaaagcccg aaaaacgata accataggat agccagccat
    65821 caaaaatcca aaactagagg ctaggatgac aaaaactgcc atcaagggcg acaagaacat
    65881 ggctataaaa atagcgatgt ggctccccaa agtataggaa gcaggtggaa tgacaatctt
    65941 gaaaggcata acaattggaa tcaaaatcgc aatagccgtt aaaagggctg tcattgtcat
    66001 aaattgtgtc tttttccgtg tattcacaag aatctccttt ttaactgcat atacactagt
    66061 atggtacaat aaaccagaca ataaagcaag aatttacttg ggtttataga tcatttttta
    66121 gttaaaagtt atagtagatt gaaactggaa tagtacacct ctgcttctaa aacattgtta
    66181 gaaatcgatt tgactgtcct gatcgatttg tcctgttctt atttcgtttt actatagtaa
    66241 agatttcatt aaaaagaaac tgtatagagc aaaatctcca ccttcaggtt tgaaagcgga
    66301 gattgttttt attttttcaa ggtttgtagt cgtggaacaa tggctagggt tagaattgca
    66361 gaaatgacca attcagcaat tgaatttgtt gagataacgg ttgccagaag aagttggata
    66421 tttccattat aaacatttcc aaataggaag aagattcctc caaggacaaa gatagtattt
    66481 gtcaaggaac caagagcccc agctaaaatc agaccagtct tgtttttcat cagtttatag
    66541 actaagtaag gagttaaacc aatcaaaata cgtgggacaa tggcaatgat agctgagtag
    66601 atgtttccgt ttggtacgaa gggagagaag aggtagcttg tcggtagaat cgtaatcgtg
    66661 ttaaccgtca agctaagtaa tcccatcaaa aatccaagtg taaccccaac tcgtggacca
    66721 taaataatgc tggcaataat gacaggaata tgaacaatgg tcggtttgat tggaaatgga
    66781 aaaaggttaa agataagtga gctcagaaag tgtatcacga gcatggtagc aaaaaagata
    66841 gcaatgggtg caatattaga gcgttttttc atcgagagtt tcctttattc tttctaaaat
    66901 aattgtgagg tcagctaaag ctcctcgtcc gtggtctcca caagctagta gggattcctt
    66961 aggagcaatc agctgatagc cgtaggtttc taatgttttc agattattct gagttactgg
    67021 atggtcatac atttttgtat tcatagcagg agctattagt ttgggaatat gacttggtag
    67081 ggctagagct gtactggtta ccatgttgtc cgcaaatccg tgagctagtt ttgcaatagt
    67141 gttagcagtt gcaggtacca cgataaataa atctgctttt tttccaagtt cgatatgatt
    67201 gacctgatca ggatagggtt ccttcatgac atccaagtgg acaggattct gtgagagtac
    67261 ctgtagtgtc aaaggttgga taaactctgt agcagcctga gtcattaaga cagtgacttg
    67321 atggccttgt ttttttagag aactgactaa atctgccgac ttataagagg cgattgaacc
    67381 cgttacagcc aagagaatgt ttgccatagt tttcctttct aagaatgata ggcttgaatt
    67441 ttttcaagga ggagttctgc aatttcttct ttagtctgga ctgtttgaag ctgatttttc
    67501 tcaacaaata tagctcggtg ctgatctgct gaaatttgag tcaggtcatt cgcgatgatt
    67561 aaatctgctt gattcttgat aagacttttt cgtgcaatgt caaccagatg atcttcggta
    67621 acatcaacca gcagtttgaa accaatcaga tgaatagtag gattccattc cttgactagg
    67681 gatatgattt tgggtgtctt tttaaggaac aaaacctgaa cctcatcagt tgaagaaatc
    67741 ttggcctgat gattctgctt gcttaaaaat tcttttagat tggagctagc ctgaacttcc
    67801 tcaagccctg tcatataaac aggagtgtag tcagaaacag ccattgagtg gatcaagacc
    67861 tgataatcct gaacacgttc ttgcatttct attagaaggt ccttggtatt ggtaatttct
    67921 cgaatactta ggttaggatg aggctctggc ttcagagctc gttttgtcgt aattaaacaa
    67981 acttcatgcc ctgcagaaag caaagtctct gtgataattt tccccaagtg acctgtagaa
    68041 tggttagtga tagagcggac gctatcgata gcttcactgg taccgcccga tgtaactaaa
    68101 attttcatag cactattgta cacaaaaata agcggtaagt aaaatgaaag tgataaattt
    68161 ttggtaaaaa cgaagattta ttatagtttc aaactataaa gccatataaa atttaagaaa
    68221 ggactctaaa agtcttaaaa acagagatat ttacgaacgt ttagagagtt ttaggatgtt
    68281 aaaaatcttg ttttgtgata taatgaatta tcatataaga ggttagagga attttgaatg
    68341 aaaacagata ttgaaatcgc acagagtatt gagttgaagc caattgttga tgttgtagag
    68401 aaacttggta tttcttatga cgatttggag ttgtacggaa agtacaaggc taaactcagc
    68461 tttgataaaa ttcgtgcagt tgagagcaat ccagtcggta aattgatctt ggttactgcc
    68521 atcaacccaa cacctgcagg tgaaggaaag tcgacgctta ccattggtct tgcggatgcc
    68581 ttgaacaaga ttggcaagaa aaccatgatt gctatccgcg aaccgtctct tggtccagtc
    68641 atggggatca agggtggtgc tgctggtggt gggtatgcac aagttctgcc aatggaagac
    68701 atcaacctcc actttactgg agatatgcat gctattacaa ctgccaacaa tgccctttct
    68761 gccttgattg acaaccactt gcaccaaggg aatgagctgg gaattgatca acgtcgtatc
    68821 ctctggaaac gcgttgtgga cttgaacgac cgtgcgctcc gccatgtgac tgttggtctt
    68881 ggtggtcctc taaacggtat tccacgtgag gacggttttg atattacagt tgcttcagaa
    68941 atcatggcaa ttctttgctt ggcaacggac atcgaggact tgaaacgtcg tttggcgaat
    69001 atcgttattg gttatcgcta tgaccgtacg cctgtttctg taggtgattt gcaggttgag
    69061 ggtgccttgg ctttgatttt gaaggatgct attaagccaa acttggttca gacaatttac
    69121 ggtacacctg cctttgtaca cggtggtcca tttgccaata tcgctcatgg ctgtaactct
    69181 gttttggcga cgacaacagc ccttcacttg gctgattaca ctgttactga agctggtttt
    69241 ggtgcggatc ttggtgctga gaaattcctt gatatcaaga caccaaactt gccaacatct
    69301 ccagatgcag ttgttattgt cgcaaccctt cgtgccctta agatgaatgg tggtgtggct
    69361 aaagacgctc tgactgaaga aaatgtagag gcagttcgtg caggttttgc taacttgaaa
    69421 cgccacgttg aaaatatccg taagttcggt attccagcag ttgtagctat taacgaattt
    69481 gtatctgata cagaagctga aattgcagtc ttaaaagaac tctgtgcctc aatcgatgta
    69541 ccagttgaat tggctagtgt ctgggctgat ggagcagaag gtggagtagc acttgccgaa
    69601 acagttgtta agacaattgc tgaaaatcca gctaactata aacgtttgta cgataatgac
    69661 ctttctgtcc aagaaaagat tgaaaaaatt gtcactgaaa tctatcgtgg tagcaaagtg
    69721 aactttgaga agaaagctca aacacaaatt gctcaaatcg ttcaaaacgg ttgggacaaa
    69781 ttgccaattt gtatggctaa aactcaatac agtttctcag acaatccaaa tgcgcttgga
    69841 gcaccagaaa actttgaaat taccattcgt gaattggtac caaaattagg tgcaggcttc
    69901 atcgttgcct taactggtga tgttatgacc atgccaggtc ttccaaaacg tccagcagct
    69961 ctcaacatgg atgttgaaag cgatggaact gtgctaggct tgttctagtg gattgaaatt
    70021 gactttaagt gagtttggaa ataatatcca agctcatttt ttctcagact gcaagcagtt
    70081 catcaataag atttatactc ttcgaaaatc ttttcaaact acgtcagctt caccttgccg
    70141 taggtatatg atactgactc
//
LOCUS       DAVBDG010000003        62278 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBDG010000003 DAVBDG010000000
VERSION     DAVBDG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 62278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.003"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(141..1010)
                     /locus_tag="VQ526_000147"
     CDS             complement(141..1010)
                     /locus_tag="VQ526_000147"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2553924.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(1012..2298)
                     /locus_tag="VQ526_000148"
     CDS             complement(1012..2298)
                     /locus_tag="VQ526_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2553925.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(2449..3186)
                     /gene="mecA"
                     /locus_tag="VQ526_000149"
     CDS             complement(2449..3186)
                     /gene="mecA"
                     /locus_tag="VQ526_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2553926.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(3386..4420)
                     /locus_tag="VQ526_000150"
     CDS             complement(3386..4420)
                     /locus_tag="VQ526_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2553927.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(4449..5882)
                     /locus_tag="VQ526_000151"
     CDS             complement(4449..5882)
                     /locus_tag="VQ526_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2553928.1"
                     /translation="MIDGKRLLFSLTIVSYTLTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(5898..6884)
                     /locus_tag="VQ526_000152"
     CDS             complement(5898..6884)
                     /locus_tag="VQ526_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2553929.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAKKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGYAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(6886..7989)
                     /locus_tag="VQ526_000153"
     CDS             complement(6886..7989)
                     /locus_tag="VQ526_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2553930.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFNVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(7979..8824)
                     /locus_tag="VQ526_000154"
     CDS             complement(7979..8824)
                     /locus_tag="VQ526_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2553931.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKKVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(9103..10377)
                     /gene="psr"
                     /locus_tag="VQ526_000155"
     CDS             complement(9103..10377)
                     /gene="psr"
                     /locus_tag="VQ526_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2553932.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(10374..11222)
                     /gene="pheA"
                     /locus_tag="VQ526_000156"
     CDS             complement(10374..11222)
                     /gene="pheA"
                     /locus_tag="VQ526_000156"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2553933.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(11219..11695)
                     /locus_tag="VQ526_000157"
     CDS             complement(11219..11695)
                     /locus_tag="VQ526_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2553934.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(11688..12971)
                     /gene="aroA"
                     /locus_tag="VQ526_000158"
     CDS             complement(11688..12971)
                     /gene="aroA"
                     /locus_tag="VQ526_000158"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2553935.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQVKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLTVESSDLKGIEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(13063..13401)
                     /locus_tag="VQ526_000159"
     CDS             complement(13063..13401)
                     /locus_tag="VQ526_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2553936.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(13412..14515)
                     /locus_tag="VQ526_000160"
     CDS             complement(13412..14515)
                     /locus_tag="VQ526_000160"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2553937.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(14525..15691)
                     /gene="aroC"
                     /locus_tag="VQ526_000161"
     CDS             complement(14525..15691)
                     /gene="aroC"
                     /locus_tag="VQ526_000161"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2553938.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLVELDVEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(15701..16768)
                     /locus_tag="VQ526_000162"
     CDS             complement(15701..16768)
                     /locus_tag="VQ526_000162"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2553939.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            complement(16787..17641)
                     /locus_tag="VQ526_000163"
     CDS             complement(16787..17641)
                     /locus_tag="VQ526_000163"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2553940.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATDTNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(17631..18308)
                     /locus_tag="VQ526_000164"
     CDS             complement(17631..18308)
                     /locus_tag="VQ526_000164"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2553941.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(18305..19468)
                     /locus_tag="VQ526_000165"
     CDS             complement(18305..19468)
                     /locus_tag="VQ526_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2553942.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     KSAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHHFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(20155..21789)
                     /locus_tag="VQ526_000166"
     CDS             complement(20155..21789)
                     /locus_tag="VQ526_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2553943.1"
                     /translation="MRLKVIKKLVDINILYSSQDANLANPRKKQAKNTGKKVNVSARV
                     LSSYIFSSLLMLIFFSTIAIHFPFEEMPVYFSFMVAILLVIAFSTSLTAFYNVFYESK
                     DLASYSPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLPSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(21770..22519)
                     /locus_tag="VQ526_000167"
     CDS             complement(21770..22519)
                     /locus_tag="VQ526_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2553944.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            22698..24152
                     /locus_tag="VQ526_000168"
     CDS             22698..24152
                     /locus_tag="VQ526_000168"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2553945.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAEDAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHMEAFQVIEVDPVDRTVELGEPFTINGWTSFTFYGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKSVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(24241..26859)
                     /gene="alaS"
                     /locus_tag="VQ526_000169"
     CDS             complement(24241..26859)
                     /gene="alaS"
                     /locus_tag="VQ526_000169"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2553946.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLVIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDVVAGKL"
     gene            complement(26881..27366)
                     /locus_tag="VQ526_000170"
     CDS             complement(26881..27366)
                     /locus_tag="VQ526_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2553947.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(27641..27880)
                     /locus_tag="VQ526_000171"
     CDS             complement(27641..27880)
                     /locus_tag="VQ526_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2553948.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(28133..29203)
                     /locus_tag="VQ526_000172"
     CDS             complement(28133..29203)
                     /locus_tag="VQ526_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2553949.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(29200..29973)
                     /locus_tag="VQ526_000173"
     CDS             complement(29200..29973)
                     /locus_tag="VQ526_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2553950.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGNDMNSFTGFSLSH
                     FKTMFGDGRLMLIVTQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(29970..30776)
                     /locus_tag="VQ526_000174"
     CDS             complement(29970..30776)
                     /locus_tag="VQ526_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2553951.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(30757..31914)
                     /locus_tag="VQ526_000175"
     CDS             complement(30757..31914)
                     /locus_tag="VQ526_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2553952.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGIMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(32074..32979)
                     /gene="murB"
                     /locus_tag="VQ526_000176"
     CDS             complement(32074..32979)
                     /gene="murB"
                     /locus_tag="VQ526_000176"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2553953.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(33091..33843)
                     /locus_tag="VQ526_000177"
     CDS             complement(33091..33843)
                     /locus_tag="VQ526_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET2553954.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFTKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALDLVLVAYGTKEIFCKKS"
     gene            complement(33853..34584)
                     /gene="budA"
                     /locus_tag="VQ526_000178"
     CDS             complement(33853..34584)
                     /gene="budA"
                     /locus_tag="VQ526_000178"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2553955.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKESGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADIATHQPEYSRDNV
                     AGTIVGFWTPQIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            34726..35997
                     /locus_tag="VQ526_000179"
     CDS             34726..35997
                     /locus_tag="VQ526_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2553956.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFDQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRAILDLAAKYSVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNTIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(36017..36841)
                     /locus_tag="VQ526_000180"
     CDS             complement(36017..36841)
                     /locus_tag="VQ526_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2553957.1"
                     /translation="MFIYEIKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGK
                     LVVATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQ
                     TGKADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQ
                     KGTVPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAV
                     ATVSLKMKDGDANVVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(36988..37641)
                     /gene="phoU"
                     /locus_tag="VQ526_000181"
     CDS             complement(36988..37641)
                     /gene="phoU"
                     /locus_tag="VQ526_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2553958.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(37653..38411)
                     /gene="pstB"
                     /locus_tag="VQ526_000182"
     CDS             complement(37653..38411)
                     /gene="pstB"
                     /locus_tag="VQ526_000182"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2553959.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(38424..39227)
                     /gene="pstB"
                     /locus_tag="VQ526_000183"
     CDS             complement(38424..39227)
                     /gene="pstB"
                     /locus_tag="VQ526_000183"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2553960.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(39238..40122)
                     /gene="pstA"
                     /locus_tag="VQ526_000184"
     CDS             complement(39238..40122)
                     /gene="pstA"
                     /locus_tag="VQ526_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2553961.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(40112..41029)
                     /gene="pstC"
                     /locus_tag="VQ526_000185"
     CDS             complement(40112..41029)
                     /gene="pstC"
                     /locus_tag="VQ526_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2553962.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(41029..41907)
                     /gene="pstS"
                     /locus_tag="VQ526_000186"
     CDS             complement(41029..41907)
                     /gene="pstS"
                     /locus_tag="VQ526_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2553963.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(42481..43785)
                     /locus_tag="VQ526_000187"
     CDS             complement(42481..43785)
                     /locus_tag="VQ526_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2553964.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(43786..44559)
                     /locus_tag="VQ526_000188"
     CDS             complement(43786..44559)
                     /locus_tag="VQ526_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2553965.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(44549..44827)
                     /locus_tag="VQ526_000189"
     CDS             complement(44549..44827)
                     /locus_tag="VQ526_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2553966.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(44827..45228)
                     /locus_tag="VQ526_000190"
     CDS             complement(44827..45228)
                     /locus_tag="VQ526_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2553967.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQQ
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(45438..46055)
                     /locus_tag="VQ526_000191"
     CDS             complement(45438..46055)
                     /locus_tag="VQ526_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2553968.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(46045..46818)
                     /locus_tag="VQ526_000192"
     CDS             complement(46045..46818)
                     /locus_tag="VQ526_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2553969.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(46829..47566)
                     /locus_tag="VQ526_000193"
     CDS             complement(46829..47566)
                     /locus_tag="VQ526_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2553970.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWKEIKKA"
     gene            complement(47571..48701)
                     /locus_tag="VQ526_000194"
     CDS             complement(47571..48701)
                     /locus_tag="VQ526_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2553971.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLNLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(48785..49174)
                     /locus_tag="VQ526_000195"
     CDS             complement(48785..49174)
                     /locus_tag="VQ526_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2553972.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(49190..49573)
                     /locus_tag="VQ526_000196"
     CDS             complement(49190..49573)
                     /locus_tag="VQ526_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2553973.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(49574..50362)
                     /locus_tag="VQ526_000197"
     CDS             complement(49574..50362)
                     /locus_tag="VQ526_000197"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2553974.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(50355..51290)
                     /locus_tag="VQ526_000198"
     CDS             complement(50355..51290)
                     /locus_tag="VQ526_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2553975.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINIADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(51420..51596)
                     /locus_tag="VQ526_000199"
     CDS             complement(51420..51596)
                     /locus_tag="VQ526_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2553976.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(51736..52443)
                     /locus_tag="VQ526_000200"
     CDS             complement(51736..52443)
                     /locus_tag="VQ526_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2553977.1"
                     /translation="MKVIKVENQVQGGKVAFEMLKEKLANGAQTLGLATGSSPLEFYK
                     EIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            52594..53622
                     /gene="queA"
                     /locus_tag="VQ526_000201"
     CDS             52594..53622
                     /gene="queA"
                     /locus_tag="VQ526_000201"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2553978.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGSHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(54198..54587)
                     /locus_tag="VQ526_000202"
     CDS             complement(54198..54587)
                     /locus_tag="VQ526_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2553979.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(54641..55348)
                     /locus_tag="VQ526_000203"
     CDS             complement(54641..55348)
                     /locus_tag="VQ526_000203"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2553980.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            complement(56048..56599)
                     /locus_tag="VQ526_000204"
     CDS             complement(56048..56599)
                     /locus_tag="VQ526_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2553981.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(56846..57670)
                     /gene="nadE"
                     /locus_tag="VQ526_000205"
     CDS             complement(56846..57670)
                     /gene="nadE"
                     /locus_tag="VQ526_000205"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2553982.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(57667..59127)
                     /locus_tag="VQ526_000206"
     CDS             complement(57667..59127)
                     /locus_tag="VQ526_000206"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2553983.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIYNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFPEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(59243..59731)
                     /locus_tag="VQ526_000207"
     CDS             complement(59243..59731)
                     /locus_tag="VQ526_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2553984.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(59721..59930)
                     /locus_tag="VQ526_000208"
     CDS             complement(59721..59930)
                     /locus_tag="VQ526_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2553985.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(60316..60795)
                     /locus_tag="VQ526_000209"
     CDS             complement(60316..60795)
                     /locus_tag="VQ526_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2553986.1"
                     /translation="MKLNKQKNRMIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMM
                     MNTSNDLFIKAIELLPFYSLPVFVASNTMSISQYLVVITTIILLYFFTLKKSKKYSF"
     gene            complement(60811..60933)
                     /locus_tag="VQ526_000210"
     CDS             complement(60811..60933)
                     /locus_tag="VQ526_000210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2553987.1"
                     /translation="MSGNIQNSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            complement(60955..61689)
                     /locus_tag="VQ526_000211"
     CDS             complement(60955..61689)
                     /locus_tag="VQ526_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2553988.1"
                     /translation="MRIKEKTNNINGGIKNVSKHYGHSIILKDINFALNKGEIVGLAG
                     RNGVGKSTLMKILVQNNQPTSGNIISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGV
                     DYNQERFRCLIQELDLTQSINKKVKTYSLGTKQKLALLLTLVTEPDILILDEPTNGLD
                     IESSQIVLAVLKKLALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHN
                     FLFEIAFSSATDRDIFITKQEFWDIV"
     gene            complement(62024..>62278)
                     /locus_tag="VQ526_000212"
     CDS             complement(62024..>62278)
                     /locus_tag="VQ526_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET2553989.1"
                     /translation="IRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNP
                     MELLTIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT        18565 a        14058 c        11446 g        18209 t
ORIGIN      
        1 acaacctcaa aacactgttt tgagcagcct gcggctagct tcctagtttg ctctttgatt
       61 ttcattgagt ataaacttct tttcacaaaa aggatatgaa accctctggt caagagtttc
      121 cccatcctat cttctattct ttattttgct tctacacgga caccttgggt atcaactctc
      181 aagtcatgca gttttccttt gaaaggttgc ttttccaatt ctgccttaat tgttggcatc
      241 ttgtcatgag aagccagaac cataactgtc ggcccagcac cagaaaggta ggttgcatag
      301 gccccatttt ctttggtcac ttgcttaatc atcgcaaatt ctcttaccaa gtcctgacga
      361 tagcgctcat ggaagaggtc tccctcgatt gcttgcccag cggtcaccat gtctcctgcc
      421 aacaaggcag caaccgctac attggcgata gaacttgcag caacagcttc cttataagac
      481 aattttttag gcaagacact acggctgtcg cgagtacgta attcatagtt tggaatgtaa
      541 gctagaaaat cacactctgg aaagtctgct acgatagcag agacttgccc ttcaacagaa
      601 cttgcaataa cgagattacc ataaatggct ggagccacat tgtcaggatg cccttcaatc
      661 ttggtcgcta actgcaattt ttcatggtct gataagttga gttgacccag ttggttggct
      721 agttcaatcc cagcaacgat aaccgagctg gaagaaccca aaccgcgcgc caaagggaca
      781 tcactggtca ttttcaagcg tcttggttgc aagtctggta caatttgcaa agcgattttg
      841 agcaagagat tacgctcatc atgtggaatc catttgccaa tctggtgttc aatcagccac
      901 tcatctcgtt cttcgcagac ctcaatttga agatacttgg ttacagctac accgaccgag
      961 tcaaaacctg gcccgatatt ggcactggtt gcaggtacaa taatcttcat cttattctcc
     1021 tagcaccttg aaggtattca agaggtcgaa ttctgaaacc ttcttcaatt cagctgagac
     1081 attttcaagc tgggctttat taatcttgtg tgtgatgata acgacacgcg ccttgtcacc
     1141 ctctttgcca tcttgaagga tttgcttaaa ggaaatatct tgagcattga agatttcagc
     1201 caacttcaag acctgacctt ttgagtctag agccaagatt gagaaatagt agtttgcttt
     1261 gacatcttca ggatttgcca agaccaagtc acggctatat tcgttgaagt ctttgccaat
     1321 agtaccatca ttcaaacgac gaacgatacg gacaatatca gctacaacac ttgttgcagt
     1381 tggtttttga ccagcacctg gtccgtagta catagactca ccaataccga tagattctac
     1441 aaagacagcg ttcattacgc cattcacact agcaagtggg tgcgctttag gtaggaaggt
     1501 tggagtcact tctgcagcaa tacctgaaga agtttcctca atagaaccaa ccaatttcac
     1561 tacgtaacca agctcttgag ctacagctac gtcttctggt gtgatattgc ggattccctt
     1621 gtgggctaca tcatcaaagg caatcttcat gccaaaggca aattggctca aaataaccat
     1681 cttgtaggct gcatcaatcc catctacgtc attcgtcgga tcgctttctg caaatcctag
     1741 acgttgtgct tccgcaagag catcatcgta agaccagcct tcttccacca tcttggtcac
     1801 catgaagttg gaagttccgt tgactactcc aagcacgcgc gtaattttat cagaagccaa
     1861 ggaatttgct aaagtacgaa gaattggaat cccaccagca actgctgctt cgtagtaaag
     1921 tgctaccttg ttagcttgag cgatttctag caattctgcg ccatggacag ctaaaaggtc
     1981 cttgttagca gtaacaacgt gttttccagc ttccaaggca cgagtgataa aggttttagc
     2041 aggctcaata cgccccatca attccactac gatagtaata tcctggtctg ataaaatatc
     2101 atccacattg gttacaaagt taaagtcatt ccctgctgca agcaagcgat ttttttcatc
     2161 ttcatccttg accaatacct tagcaacttt gatatctgaa tgtgctgatt gattaatttt
     2221 tcctccattt tcctttagga ggaaaggcac accacttgca acggtaccaa atcctagtaa
     2281 agcaatttta actgtcatct ttatctcctc gaaattttct aatatagcca ttataacaga
     2341 attttgtgaa aattcctatt atagtaaatc actatttcag tataaaaaga aaaaacgaat
     2401 cagacgattc gctcttctta aaatctgaaa atagctttcc agaaaggatt agccgatttt
     2461 ttgcagattg agcactgcat cgtgactcat caagacttga ccatactctt gtaagactga
     2521 gcgactgata tcactatcgt ctgcaaactc gcgcatacgg gccaacagcc aagctggata
     2581 tgggcttgga tgattttcaa tatccactaa aatggtcaaa taatagcgct cgttcatttt
     2641 gtagagttca gaagtttcca tttcaaaagt cactgtcttg gcaaaagcta ccaagtcagc
     2701 caacttagca aaagaaagga tgtagtagat gtaaggttct ttcttactct cagcttcttg
     2761 ttcagcctgc tcttgctctt cttccttggc ttcaacttgc tcaagagatt gaatggcttc
     2821 gatatcatcc ttggttttgt ctgctatgct tttttccagg gttttgataa attcatctgg
     2881 agacatttga gccaattctt ccatatctgg caaatccgat aagtcttcaa aatctagatt
     2941 ttggtcaatc tttgacttgg tcacaaagac atctacctta tcaggttttg gagtcacacg
     3001 gaagctcaac atgcctgtat ccagaaagct atcaggcatc tctagctcat ccaagatagc
     3061 ataaaagaac tcttctgttt tttcttgagg aacgagaaag tcagcaatct ccattccacg
     3121 atccatcaaa tcctctaaag acatcgtgat ttttaaagtt gtatcactaa tttgtttcat
     3181 tttcattgct agtaacctca tactttcagt tctatctatt atactagatt tttacgattt
     3241 tatcaaaaga aggctcctct atacggatag attttcccta gggtctttct ataggagact
     3301 ccaaaagaaa atttctgcag acagatagaa aaagccttca aaatcggctc ttagccgatt
     3361 ttgaagacct tatacatcag aatacttata atttaaaggt tgctacaccg aggatagaac
     3421 gatttaagtt tctgagaatt tgaagacttt gctcaaattt cttataacga gtcactccgt
     3481 actcttcaac aagaaggact gtatctcttt ccaaaagaga tgatacatcc tgtaaatcta
     3541 caaaatgcat tccttttaaa gcttcttgac tctgtttcaa tttatctagg atagctttat
     3601 ttgagctaac gatggtcaat tcctgtccag tatttttgta tgacaaaaca tctgctaggt
     3661 tagcaattgt tgtaatctct gttacaaaat caatttgata ctgagaaaaa tcacctactc
     3721 tattgattgt tggattaaag agataaacta acacatttcc catcacaacc aaaatcacac
     3781 aaaccactcc aataacaact aaacgaagaa tcagattttt cacatttaag ccaagcgctg
     3841 tttcaccatt tgcgttcaat tctttagagt tgatggtttc cagtttttca attttcacat
     3901 ttgcataggc atgtttaaat ttctcaatca acccatcaat ttttttctct aacaagtcat
     3961 tggcatcttt acttgatgtc aaaattttca caccaacccc tgcatcgtca atcatatagt
     4021 agacggtcaa ttttttccac caatagtcat tcgttgaatt tttcaaggtt gtttctgtcg
     4081 tgtctaattc actggcaatt tttttcaact cactgggttc tacatcattg aaaagataag
     4141 ctccattcaa attaccatca atcaatttcc cataaaaatc actataacca ccaatttgat
     4201 gattcaaaat cgttttggcc gactcttttg gaggagtgat tttatagata agataagttg
     4261 aataacttgt tgtatctttg acagtgtttt tattcctaac tgctttaatt gtaaatggta
     4321 cagcaatgag agcaaataaa gcgatgagag ctaaaatatt tgcttttcgc tttttataaa
     4381 gatttgcaaa caaatcagct actgaataat gttcaaacat gatttttttc tcctttgttt
     4441 agtagatact agttttcctt tgtaagcatt tttgctacaa atataatcac aagaacaatt
     4501 ccccagaatt gcattgtaaa taaattgaag aaactttctg aaaagctgct tcttggcata
     4561 aagaatagat tattcaagat gagtagggat aaagcaaata ggattgtcct tgagcgatag
     4621 gctacttgca gcatggctat aaataatacg ccgagtaaga aactaagcag aaagactcca
     4681 atcataccat agtcggtata caactccatg atataactac ttccgatacc atgccctttc
     4741 aagtattcct tgttcaagac aagataggat agattgtggg cataactatt actatcaata
     4801 gctagttcca cactattggt tgtatgttca aaggcttttc ctccgaaaat ggctcccaaa
     4861 ctcccccttg caaaataatc aagaacagga ccaaaagtaa aattacggaa atctcggtaa
     4921 gggaggctac tgttaaatag aaaacctcga gccagaacac caaaactagt tccttgttta
     4981 tagataaagt caagtaagat atcccagaaa cctgtatggg aaacttggac attatcccgt
     5041 acataattaa gtactcccat cgctaacatg agaataggag aacctacaaa aatcgctaac
     5101 ttttctttaa acccaatcca ttttcctttt tcagtttgct cccgcataaa gtaataaaca
     5161 aaagcaaata aaatacttaa aataaaggga tttcgtgtcc caattgccaa atgaatagta
     5221 ttagctgcaa taaaggagac aagcactgct gtggcctgca atttctttgg cttggttgcc
     5281 agatacatac acattgcata gaccgtaaag gtagacaaaa tgtaggtaaa ataaggcagt
     5341 ttactttcaa aatttgcata gtaggcatag taggaagtct gcaaacgata caagagccgt
     5401 tcaaataacc gaatgaaata gaaaggatag gttagaagaa aaactcctag tgatacaaag
     5461 cgtaaccgct tgatataaac ctcttttaga gaatttccta tatttgctac ttttattttc
     5521 ttcctagcta tgaagtaacg agccaggacg cctcctgtgg tcaagcccag aatcgaaacc
     5581 atgacaacta taaaggcaaa acgataggct attggatgat aggtatccaa agcaccatcc
     5641 ctaaaataat caatggtcgg tcttgatacc agaaatacaa aaatggttaa atagaaaata
     5701 aaatggatta agtaatactt gatatcattc caacaagcaa ttaagctact aaccaacaag
     5761 aacaataaag tagaaagtaa gctaacatta ttattattaa acagatacac aattccactt
     5821 actagcgtca aggtataact gactatggtc aaactaaata ataatcgttt cccatcaatc
     5881 acttggtcac ccccgttcta atgtaatttt ttagattttt caatattttt cagtaataag
     5941 aatcggtata aggaaatatt tatgaatagg gccaaagcac taattcttct ccccttacgg
     6001 aaaattggat tcctagaaat agcaaaagca tagcctttta aaaaacgatg aatctgagaa
     6061 taggcttcaa actgtttata ctgatcatct agcaacatct tatccagaat aaagaagtga
     6121 gcataggcca atctgaaaaa agcgacctct ttcaagtcag gatagttttt cacaacttca
     6181 ttataaaact tttggtagat atcaatatag gctaaatcct tctttgcata gggtttggtc
     6241 gtaatactat cccctctatg gaaatagtaa taatagggtt tagtattaac cacatacttc
     6301 ttggccaact tgattaaatc aaaatggtaa taggcatctt cgtaaatcaa ccccttagga
     6361 aaggataggg cagttgcaat ttgtctcttg attagcttat tgcaaatcgt cccaggtatt
     6421 ttttcaccta tgaggtattc ctttagaaat gtttgagaat cacagacaaa atagtcatcc
     6481 tgattggctg actgtgggct ttcatcatta gcatagacat tcatgacacc acagcttgaa
     6541 acatccgcat cttcttgaac taattgctca tataagctct gaatcatttc tggatggata
     6601 taatcatctg agtcaataaa aatcagataa tccccgtgag cctgcttcat cccatcattt
     6661 cgtgcttgcg acaatccttc gttcttttta tgaagcactg acaccctgtc atcttgttca
     6721 gcgattgaat cacacaagcg accactttca tctgttgcac catcatcaac aagaataatt
     6781 tccagatttt gataggtctg cttctgaatg gaagctatcg atttttctag gtactgcgcc
     6841 acattataga ctggcacaat cacactaatt aatgcagttt ccatgctact cctctaatag
     6901 tttttctact tgttcgattt gttttgtaat tgtaaattgt tgaatgaatt ggctagcctc
     6961 atcgacatta aagtttgagg cagaagtcat gtaattagta atcgcctgag ctgcctcttg
     7021 attgctctca atgatttgtc caaatcgtcc ttcttgggat aattcctcag cccctccaac
     7081 gtccgtagag ataaaaggga gtcccagact caaggcctcc acatacactc caggaaagcc
     7141 ttcttgttta gacatagaca agagaacttt cgtctgagat aaatactgat aaggattttt
     7201 ttgataacca aggaaatgta catagtcctc aatctcatac tctttgactc gttttttcag
     7261 ttcctcttcc atatcaccag ccccgataaa atagagatga tagttttttc cctcttggtg
     7321 taataatcgt atcacttcca ctacacggtc agaaccctta ttttcctcaa tccgtccgat
     7381 agtacagata ctttgaggag caatctcgat atcgatcttc tcttgagatt tttctagaat
     7441 agtctgaaaa tcatatccat tgtagattgt ctgtaattta gaagcataat ctggataaac
     7501 ttccttgata gaattgctgg tcttttttga aatccctaca attgtattcg cagcatccaa
     7561 ctggcttcta tgtgattctc ttttagagct atccttaaga agttcttcaa tacttccatg
     7621 aatccaagat atcttcttga cttctcttct tttagagaac aacagtggtg gattcataat
     7681 ggtaaaagaa acttcaacat cataatcatc ttttacaagc aaacgacgag tcagtcttgg
     7741 aaaataaatt ctcattctcc acaaaaaagc tcgtaaccat ctggtttggc gataatcttg
     7801 aagggatttt aaaatgcgta catgctttgg aacagattca tatcccttgt caaagtgctc
     7861 catttcaaga atatcaatat catacttttc tggatccaga tttgaaacaa tggttgatag
     7921 aatcttctct gcaccacctc caagagaaaa agaccacata aaaaataaga tttttttctt
     7981 agccaccata ttctcccttg tattctgtat aagacttatc catatcagcg atgacagcat
     8041 catgatgcgg taccttcttg tctgctggtg gaggcgtcat ataatcccca aaagcagttc
     8101 tgagatagac atcatagccg attggaatag gcatctctgt tccttcaaat ggcaagaaaa
     8161 gattgtcttc aaaagacgtg attgggtact tgtttctcat gtagccagga cctgagcata
     8221 attctgtaat gccatcacaa tcagccaaat catacttagt catttctttc tcagcttttt
     8281 tccagatgcg ataacggaga gattttggag tcaaacccag taaaatgcga cttccccatt
     8341 tcatgagagc accatgcttt tctggaatag tttgcgcaca aaagagtgaa taaatcaagg
     8401 cccaacgaac ctgttttttc cgctcagctg gatttttcgg ataataatcc aaaggcaaaa
     8461 catccaaggc cagaccatgt ggcaaatcca aatcctgctg ataaggcttg atacaggtgg
     8521 tttccttgtc acgaatggta ataaaaagat tacgatcaac aaaatccttg tgactctttg
     8581 acaagaaata acgttcatct gcataacgag gccataattc tgctaatttc tcataatctt
     8641 tacgaggcat aaaaaagtct aggtcgtcgt cccaaggaat aaatcccttg tttcgaaggg
     8701 caccaatagc gcctccgcca cagagataac agagcaaatc atgttcttta caaaaggcca
     8761 caaaatattc agccatctcc agactacgag cctgaattgc ttttaaatca gtcatattgt
     8821 tcattattct ttctatcgta tcgtttcatt ataccacaaa caaggagtga aaatctattg
     8881 cagactgtaa aaaatcaaag cctgactgct atccaaatag ctatcaaact ttgatttttc
     8941 tgtcttatac tcttcgaaaa tctcttcaaa ccacgtcagc ttcaccttgc cgtaggtata
     9001 ggtaactgac ttcgtcagtc ttatctacaa cctcaaaact gtgtttttag cagcctgcgg
     9061 ctagcttcct agtttgcact ttaattttca ttgagtatta tcttatctca agcccatttg
     9121 agcgagcttg gtttgatatt tgttttgatc aaccagcaag cccaagcctc cataaacatc
     9181 ataggcatct acccagtcac ccagttctgg aatcgtcaat ttttcaatac cattttttgc
     9241 tccatccaaa acagataaac cgtttgttag gaggaaagta tagggtacgt tggttgaggt
     9301 catagcaaaa acctttccaa gagcttcaga accagtgaaa agtttagtgg gatctttaat
     9361 ttgctctaaa attgctgtta aaacttgttg ctgtcttttt gtacggccgt aatccgcctc
     9421 atcatcatca cggaaacgag cataattgag cagggtcgag ccattcatct gctgttttcc
     9481 gactttaatg gtttgggttg gagactcagt ctcggtagcg tataaatcat ctccgactgt
     9541 agcttctgtt aggggacgcc cattcaatgt tgaaaattga gcatcaatcg tcaccccatc
     9601 agggaaaagc gtgtcaatcg ctgtggcaaa ggcctggaaa tcaaccaagg cgtagtactt
     9661 aatgtccaag tcaaaattat ctttcaagac ttggcgaacc atttctgccc ctttttgccc
     9721 ctcttgttct cctaactcgt aggctacgtt taacttgtta tctgtctgtt ttctaccgtt
     9781 aatcacttga ctataaccat ctatatagac caaattatca cgcatgaaac tgactagctt
     9841 cattttctta tctgagcccc cgacatttaa taccataata gagtcagttc gtgtctcaac
     9901 actgttctgg ccgattcgac catcagtacc catgattaaa atattaactc catctctagt
     9961 gtcctgacca ttaaagactt ctacttgagc tgcccgggca tcagcagttt tctttgcgct
    10021 agcatcttgg taaccacgca aaaacatgaa taccatggcc aaagccacac agaccaaaag
    10081 tgaaaaaatc accataaaaa ttcgtttaag acggagcttc cgtcttttct tttttggagg
    10141 gaaagagagt gcttgtgatt tggattgtga gcgactccgg ttcgcatagc ttggtaagtc
    10201 aacctgctct tctctttctt gttccaagct agagctacta tttcccctag caagagttag
    10261 cttttcttgc aaatagacaa actcattttt ttctctctca ttgagatagt gaatattttt
    10321 tagcaaataa tcataacgca actgctcatg atgacttaag ggattttctt tactcatctt
    10381 ctctcctttc catggtctga tattggataa ataggatagg cacccagaat tttatactgg
    10441 attccaatcg cttctaattc tttttgggca aagtggacca agtccttatc ggtataatcc
    10501 acatcgataa tgaaaaagta ttcacccagt gctgtcttga gtggacgact ttcaattttt
    10561 gtcaagtcaa ttcctcgcca agcaaaggtc gacagggcct tataaagtgc acctggaagg
    10621 ttgtcaggta atgtcaaggc caaactcatc ttttcagttt gtgcttgcaa gggaatacta
    10681 ggcttttcag ctcctagaac ccagaaacgt gtgaaattgg cttccatttc ctgaatatcc
    10741 tcggcaatca gttccaatcc atattcttca gcagaacttc taggtgcaac tgctgcaaag
    10801 ggctggtctg gatgttcgga aataaaacgg gccgcataag ctgtactagc tgttacctcg
    10861 atttgagcct ctggatattg ttcatcgatg aatttctttc cttgagccaa ggcctgtgga
    10921 tgtgaaaaaa tcttttcaat cttagtatgg cctggaacca ccatcaactg ctgatgaata
    10981 ggctgaacga tttctgctac tgcttggatg tgagcctgat gaaaaagata gtccaaggtt
    11041 tcatgaacac taccctcaat agaattttca actggcacca cagaatagtc cactaatcct
    11101 tgctcataag ccttgatgac atctgtaatg ttggcaaaag cctgcaattc ctcatgagga
    11161 aaagctgtct gcacaacgtg gtgtgaaaat gatcctttgg gacctagata agcaattttc
    11221 atcttagttc ctctataatt tcctctgggc ttagcttggt cacatccaaa acccgactag
    11281 ccacttcctc ataccaagcc tgtctttctt gaaaaatagc tactagttct tccttgctat
    11341 tatttagaaa aagcggtcgc tgattgtcct tatcagctgc gatacgttgg tagagggttt
    11401 caaaatctgc tttcaggtag atgttatctg tattagtctt gagtaagtca cgatttctct
    11461 gagaaataac cactcctcct ccagttgaca cgacttggtc tgtttgtagt aaatcagcta
    11521 ggacttctga ttctacctga cgaaaggctg cttctccctt ttcagcgaaa aaattcgcaa
    11581 tggacatacc taggcgcttc tcaatcagag catccatatc aaggtaatta gggtccaagc
    11641 ctcttgcaat agtcgatttt ccagccccca taaaccctaa taacacctta gccatgaatc
    11701 aagctctcca aatcatcaaa gaaactagga tagctggtat tgatggcttc tgcacggtca
    11761 agctccacct ctccatctgc aaccaatagg gctgcgatag ctgtcatcat gccgatacgg
    11821 tggtcaccaa acgtattgac tctagcaccg tgaagagctg attttccttt gataatcatc
    11881 ccatctgccg taggagtaat atctgctccc atactattta aggcgtctgc cacaacctga
    11941 atacggtctg tttccttgac cttgagctcc tcagcatcct tgataactgt tacaccttgg
    12001 gcttgggtcg caagtagggc aataataggc aattcatcaa tcaaacgtgg aatcaaagcg
    12061 ccaccaatct ctattccttt caagtcagaa gactcaacag tcaaggtagc agatttagcg
    12121 actggatcga tttcagttat ttccaatttt ccacccatgg cacgaatgac atcaataata
    12181 ccggtgcgag tttcgttgat ccccacattc tgcagcacta gacgagaatt tggaacaatc
    12241 aaacctgcga ctaaccaaaa ggctgcactg gaaatatctc ctggtacgac caccttctgt
    12301 cctgtcaatt tttgtggccc ttggactgtg attttcttac cgtccacact taaatgacca
    12361 ccaaattgtt tcaacatatc ttcagtatga ttacgggtgc actctttttc gataataact
    12421 gactccccct taacttgtaa ggctgcaaac atcaaggctg acttgacttg ggcagaggca
    12481 attggcaact cataatgaat aggtcttagg tttttcgtcc cttttaagcg aaggggaggc
    12541 aagtctcgtt cagtttgccc tgaaatgctg acgcccattt ttttcagtgg aagggtcaca
    12601 cggtccatag gacgtttgga aagactatca tctccaaaca tctctacttc gaaatctgca
    12661 ccagcaagga cacctgaaat caggcgaatc gaggtgccag aatttcccat attaagggca
    12721 ttttgtggcg cttttaagcc agccatgcct acaccttgaa tggtaataac cccatcttta
    12781 tcctcaattt caacaccaag gtcacgaaaa acctgcatgg tcgaaagaac gtcttcacct
    12841 cgcagaatat cataaacctt ggtctcaccc tcagccaaac ttccaaagat aatggaacgg
    12901 tggctgatag acttgtcacc tgggacgcgg atactaccat gtaaatggcg aatgtttgtt
    12961 tttagtttca tactggacct catacttgca atacttttac ctattttatc ataaaaagcc
    13021 agaaattcct taaaaattcc tgactttagg atcgttcttt tcttatttca gcaattctga
    13081 aactggttca aaaacaattt tttcaatgtc agaaaggtaa atggccaatt gttgttgctt
    13141 ggtaaagaat tctgacaaga ggctatttcc ttgaatctgt ttaccaaagc cttccatctt
    13201 agcttggaag gacgcatctg gcatttgacc tgtctgtgct agtttttgaa tttcctcttg
    13261 aaaggcaaga tattctgtaa agattttgct tgcctcagca tctgctgcaa tcgcatcttt
    13321 agctgcttta acagccttgt attctggtaa tccgcgtaga ccgcgactga gttcgtttgc
    13381 actatcgtaa atatttgaca tgttcttctc cttatttgat gacgactgta tagtcggtat
    13441 tttctgttat gagatgctca gctctttcca agtcttgagc atttttaaat gaaatttgta
    13501 ggattccgtg aatatcctca cgattttcct cgttgatgtg gatgttaacc aaggaagttc
    13561 cacgtagcag ttccaaaatc cgcaggatga catcttcttc atcaggaacg tcaacataga
    13621 ggtcgtaaga gctatccaca ccaccacgct tatggatttc cattgtctgg cgttgttcac
    13681 gcgcttggtt aaaaaagttc caaatttgct cttcatctcc cttactaatg gcctgaccaa
    13741 tcacttccaa acgttccttg aaatcctcaa ttctatccag aatggtctcg ctattggaca
    13801 agagaatgga ggtccacatt cctggctcgc tttccgcaat tcgggtcata tctcgaaaac
    13861 cacctgccgc aaagcgcctt gccatctcat gctcttgagc atagaccgca gtctgctcca
    13921 tgagactaga agccaaaata tgaggaaaat ggctaatctg agaagtgaca cgatcatgct
    13981 ccttggcatc aatctcgata aaacgagcat gaagacctga aagcagatcc ttcatttcct
    14041 taagcgtgtc ctgacttgtc aggcttgaag gtgtaaagat ataataggca ttttcaaaaa
    14101 gattgacatc tgccgaagca gcccctgtct tgtgactacc agccatggga tgggccccga
    14161 caaagcgaac agacttgcca gccaaatact gctccgccgc atccacaata gttgacttgg
    14221 tcgaaccagc atctgaaata ataacgcctt ctcgcaaatc caaattggcc aactccttaa
    14281 tgaaagcaat agtttgtttg attggcaagc tgaggataat gacatctgcc aaaggagcaa
    14341 aactagcaaa atcatccgtt gcacggtcaa tcatgccttc tttcaaggcg atatctctcg
    14401 aagcttgact acgattataa cctaaaattt cataatctgg atgatcgcgt ttgataccaa
    14461 gtgccataga ggctccaatc aacccaagac ctgcgatata gattgttttt gccataggaa
    14521 ctccttaata gttctttgta tagtctcggt gtttggctac cgcttctttt agttcctcaa
    14581 gattatctga tgagaatttt tcgaggattt cttgcgccag aaccgttgct acaactgctt
    14641 ccatgaccat tcctgcagct ggaagagcag tcggatcact tctctccacg gttgccttgt
    14701 aaggttcgtg ggtttcgata tccacactca taagaggttt ataaagagta ggaatgggtt
    14761 tcatgacccc acgaacaacg atgggttgcc cattagtcat accaccttca aaaccaccta
    14821 gattattggt acggcgagta taaccgtctt ctttagacca gagaatttca tccataactt
    14881 ggctgccttt acgataacca gcctcaaagc caagaccaaa ttccacccct ttaaaggcat
    14941 tgatagagac aacagcttga gccaatcttg catccaattt tctatcccat tggacatagg
    15001 aaccaagacc aactggaacg cctccgacga ctgtctccac aaccccaccg atggtatcac
    15061 catcacgttt gatttggtca atatagtcct tgatttcctg ttctcgttct tggttgacaa
    15121 tagaaacttc agactgggca gctctttgct taatttcagc gactgtcaga ttttcaggaa
    15181 catcgatttc cttgccacca aagaccacga catggttggc aatctccaca tccagctcaa
    15241 ccaagaggcg tttggctact gcaccaactg ccacccgcat ggtggtttca cgagctgatg
    15301 aacgctccaa agaatttcgc aaatcatcaa aacggtactt aatcccccca accaaatcgg
    15361 catgacctgg gcgaggatga gtaattttcc gcttgctttt aaggcggtct tcaatgtcct
    15421 ccgcagacat gatgtccagc catttctggt ggtccttatt gatgacatcc atagtaatag
    15481 gcgcccctgt cgtcttcccg tggcgaacgc ccgaagtaaa gacaacctgg tcactctcaa
    15541 tcttcatacg accaccacga ccgtagccac cctgacggcg tctaaggtcc tcattgatat
    15601 cctcagctgt caatggaagt ccagctggaa ttccctcaat aatagctgtt agacgggggc
    15661 cgtgtgattc tcctgcagtt aaatatctca tacactctcc ttattttacc aagtagtctt
    15721 tcatctcttc cagagaaact gggtgaatga tcgctgaacc aagctctggc accaagacca
    15781 atttcaaggt gttaccacgc gctttcttgt catgagtaag agcctgataa agcttgtcaa
    15841 cttcccaatt ttcatagtca acaggcaagc cgaatttctg acacatctct gtgatagatt
    15901 gggtaatgcc agctggcatg aggccttttt cctcagcaac cttggaaatc tgtaccattc
    15961 ccatggcaac agcctctcca tgcatgacct tgccataacc ggcagtcgct tcgatggcat
    16021 ggccaatagt gtggccaaaa ttgaggtaaa gacgaatacc attgtccaac tcatcttcaa
    16081 ccaccatctt gcgcttcacc tgacaagaat gttcaatcaa ggtctctgca tgttccaaaa
    16141 tactctcaac agaaccattc agtcccgtca agagagccca cagttctgga tcctcaatca
    16201 agccatactt gataacttca cccatccctt caatcaactc tctttttccg agggtttcaa
    16261 gaacaagtgg atcaatcaga accccatctg gttgggcaaa ggtccccacc atatttttag
    16321 caaatggtgt gttaacgcct gtctttccac cgatagaaga atcaacctga gctgtcaaac
    16381 tagtcggaat ctgaacaaag tgaatacccc gcatataggt agaggctaca aatccagcca
    16441 ggtccccaac aacgccacca ccaagagcaa cgattccatc gctacgagtc agaccttgct
    16501 tgactagaaa ttcatagact ttctgaacag tagttaaatt ctttctttct tcaccttcta
    16561 agaaatcaaa aacagctacc tgaaaaccag catcttctag gctgagcttg accttctctg
    16621 catagagaga ggctacatgg ttatctgtca caatgactac cttttgcggt tgccagagtt
    16681 ctcgcaacca ctgaccagcc tgggccatac aacctttttc aatctgaata tcataaggat
    16741 gatgaggaat atcgattctg attttcatag gagagtttcc ctttctttat tggtattttt
    16801 ctgttaaaga ctgccaaatc tcttctgtcg gcatttcctt gcctgtccac agttgaaaag
    16861 cttctgcagc ttgatagagt aacattccca gaccattgac tgctggattg ccctgacttc
    16921 tagcccattt caaaaacggt gtttcaaagg gttggtatat gatatctgca actaaaagag
    16981 tttctggtaa gactatgttt tcaggaacag gagaggattg gccatccatg cccacactgg
    17041 tggcattaac tagcaaatcc gactcggcaa tccttgcttg cagttcagaa acatattcta
    17101 aagcacacaa atccacttta aaacctgtct gctcctgtaa cttgtctagg taaggtcttg
    17161 ttttttccat agaaacggaa cgaacaaaga ccgaaatctg actgacgcca tccaaaatag
    17221 cctgtgccaa gattgattta gccgcaccac ctgcacccag cagggtcatc tttttacctg
    17281 aaattgtaaa agaaggcaag cacttaaaaa atcccttgcc atctgtatta tatccaatta
    17341 aattgccatt ctcattgaca accgtattaa ccgcaccaat caagcgcgct tcatcgctca
    17401 gcttatccaa ataaggaatc acctgctcct tatagggcat ggacagattg atgccaaaca
    17461 tctggtagcg acgaatattg gccactgttt ctaccaagtc actcgcttca atctcccaag
    17521 ccacataagc accgttggta tctgtcgcct caaaggctct attgtggatg aagggagaaa
    17581 tagaatgctt aataggattg gcaacaactg cagctaaacg tgtatagcca tcaagcttca
    17641 tccaaaatct ccctgatttt tttcatgcta gctagagaaa tctgcccagg ggcactaacc
    17701 tcatccagac tggcaaaaga ccaactcgaa ccagtcacat ccgcagtgat acgagagacc
    17761 ttgcccacct tacccataga aatggtcaca tattcctgtt caggattgag ggttttaaag
    17821 cctcgtgtat agttcatcaa gtctaagaca tcctgctccg tgtgagccat caccgcaacc
    17881 ttaacaagtt ttggatttag gatcgtcaac tctgacaaga tttccatcat gttctcaggt
    17941 gtttcttgga aattatggta actcaaaaca agatttggga agtccagcat ttcctcaaaa
    18001 acatccttgt agctatagta ctcaaaatca atatagtctg gttgatagag ttgcgcaact
    18061 tccttgatta gatggatata ctcttctgga gaaaggtcga tttctccacc ttcggagcga
    18121 gttcgtagcg tgaaaaccaa ctcacggcct gcgaattttt caaaaatggc tggagctacc
    18181 tgcaaaatcg cttctttagg cagatagtcg gcacgccatt caatgatgtc ggcatccagg
    18241 tacctcgtgg catccagagc ctgagcctcc tctaaacttc ttggcattac tgaaacgatt
    18301 aatttcattt actaaccttc atactaatca ccttgaggta attactactt tcatcttttt
    18361 tattataggc aaaatctgct ggaagaccat atttgtttaa aatctggtaa cttcttcctg
    18421 caaaaccttt atcaatttgt tctgtaaatt tctgacggga aacattggca gcattggtac
    18481 tggcaatgat aatccctccc ggatttaaaa tctcaagact ctgggaaatc aacttgtgat
    18541 aatccttggc cacagagaaa gtttgttttt tattccgagc aaagctaggc ggatctagga
    18601 caatcacatc gtaggtcaag tctttgcgtt tggcatattt gaaatactca aagacatcca
    18661 tgactataaa atgatgctcg tctgtgctga gcccatttgc ctgaaaatgc gcttgagaca
    18721 attctcgtga acgtttggct agatcaacag aagttgtctg gctagctcct cccatggccg
    18781 cagctactga aaaagccgct gtgtaggaaa acatattgag caaggattta cccatagcca
    18841 agccgtcaac taaactaccg cgaacctcat gctggtctag gaaaatgcct gtcatcaagc
    18901 catcattcat aaagacttga tacaggacac cattttctaa aacagtgaaa aagtcaggtg
    18961 cttcttgacc ataaacatgg gcagatttat agtccaaacc cttaaagcgg attttctcat
    19021 aagctcctaa aacctcaggg aaaacctgtc taaaggcttc tgatatagtc tgacgaatct
    19081 gataaacata agagttatac caagaaaaga cggcgtagtc gccataaagg tccactgtca
    19141 gacctccaaa gccatctccc tcttgattaa agagacgaaa ggcagttgtc aaatcatctt
    19201 gatagtaggc gtttctcttt tctttggctt ttctaaacaa cgtttcaaag aaagtttgat
    19261 tgaaggccac cttgtctttg ctgacaaacc agcccaagcc cttgttttgc tgagaaaggt
    19321 aggcagtccc aagaaagttt ccttcctgac cctgcacctc tacttcctga tccttaagat
    19381 tgacattctc aagatcactg gcttctagta aaactagccc cttagcaagc ttcttttcaa
    19441 cccttttgct gactcttatt ctattcataa ctaccattat atcaaacttt tagacaattc
    19501 tcaaaaaaga aactaccctt gcttttttac tcttctttta aaaaatggta tactagactt
    19561 cctgcaaaac taggaagtaa atgtgtaaga atcacaataa aaaatgctct tccgtcttgg
    19621 aggagcattt ctttttatca acgaaaatca aatagcaaac tatgaaacta gcctcaggtt
    19681 aactgtgaga ttataggtag agaggttgaa tcagcaatat gtgtctgtca aatttagtga
    19741 caaaggtagt agaagaaaga tgaagaaata aatctgcttc aataggtatc tggaaaattt
    19801 gattttatag agaagccttt tgttacaaac tcaatatact atcaataaat aatattatag
    19861 aagcaacaat aattataatt tcacctatct gcatcattct atttcgaact ctaaatatat
    19921 gttctatcaa aaatacttgg atcacacaca ttataggaat taacgttttt gaaattgaaa
    19981 aatatcccaa taaataaact ataaacaaca aaaatagaac tatgttatat ttcttattca
    20041 aaacattcct ccctatatat ttttgattac caatcttaat catttacaac tacattctaa
    20101 caaactataa aagcgtttgt cgaactgaat ttatcaagca agcgaccaac cagctcatct
    20161 tttttctatt tctgccaata tgcgtgacag gtaataatga tagccaaaaa tagcaagagc
    20221 aagcaggagg ataagagctc ctactcccaa gctgatggca aggatagggg agagagactg
    20281 aaccaagaat atactcccaa ttacaagggc catcaggatt gcactataaa taaacaataa
    20341 aactatggcg actatgccat ttgaacgatt caccaggtcc gtaatgctac tccaattggt
    20401 tgacagattt ttcacatcct taaaataatg gtgacaagaa aggatgacac tggcaaggat
    20461 ccatgccaca ataaggtaaa tcatcgaaat gatgggcaag cctagatata gagaaagacc
    20521 aagtaaagtc agaattggta aaaaggactg aacagcaaag ataatccaaa atttcacttt
    20581 cacataacga gcaaagtcaa agggtaaact cttcagaaaa tcaacatttt ccctctccaa
    20641 ggacaaggca attgaaggca ggctggtgat attgttattg acaactgcta taaagagagc
    20701 tataaaaaac aagggtaacc agtatggagg atgaatgtct ggaactatct gagaatctcg
    20761 gattttggaa atcagaccga tcatcatgag ataaggaagg aaagcacttg taaaaagcac
    20821 tgtaaccacg ccagtcccct gtcccaagag ggtgaggtgg tagcgtaaaa ccatgcggaa
    20881 aaatcccttt ttagtggttg aaattctctc cttgctgcga cgttcttttt tgaccttctc
    20941 ctcactatta agcaggatca cgtcataaaa acgaggaagg accttctttt tggtcagata
    21001 aagcaggaag agagttagtc ctatccaagc gagcagaccc aatatggctt ctattgaaaa
    21061 aggctccact gctattttgt aaaagatatg aagaggataa aggagaaatg gaatgtctct
    21121 aactttgtca acaatacttc caaaagtcga ctgaagaaag aagataaata ttaaaggtat
    21181 gagaactcct atcccaatca tcacattcga aaaaatagac tgatactttc tgaagaccct
    21241 agtctgagcc aagaaatgta ctgccactac cgtcactaaa gtaacagaga caaataataa
    21301 ggtcaaggac agtagcatca aaggcaaacc cagccaaaga gaaggagcta gactaatata
    21361 gagggctaga aaataagcta ggattggtac aattccagtt agagctggca agaggacaga
    21421 cagtccttta gcaattataa tctctgattc tttaaaagca tagggactat aggaggccaa
    21481 atccttactc tcataaaaga cattgtaaaa tgcagttaaa gaagttgaaa aggcaatcac
    21541 tagtaaaata gcaaccatga aactaaaata aacaggcatt tcctcaaaag gaaaatgaat
    21601 ggctatagta ctaaaaaaga tgagcatcaa gagactggaa aaaatgtaag aacttaggac
    21661 tctagcggaa acatttactt tttttccagt atttttagct tgcttctttc gtgggttagc
    21721 cagattagcg tcttgagatg aatagaggat attgatatca actaattttt taatgacctt
    21781 gagacgcatc tgaaacctcc tcttttctac cagcaaggct aaggtagata ctttccaaag
    21841 actggtctgg gttgtctttt ctcaagtcct ctaccttacc acaataaatc aaatgcccct
    21901 ttttcaaaat agcaatccga tcacagactt gctcagctac ctctagcaca tgagttgaaa
    21961 acaaaactgt cttacctttt tgcgcatgct ccttcatcat ttgcttcaaa tcaaaggcgg
    22021 cctggggatc caaaccagtc aagggttcgt ccaaaaccca aatatcagga tcagacaaga
    22081 gtgccccgat gacaaagact ttctgacgca ttccgtgaga aagagtttca ataacctgat
    22141 agcgattttc agcaaaatca aaaacgttca atagcctagc tagactagcc tccaagtcag
    22201 atctactcag atcataggat gaggcgatca attcccaaaa ttcattggcc gttaagcgta
    22261 aaaataagtc aggcgagtct gctacgtagc caatctttcg tttaatagcc aagcgatttt
    22321 ccgataactc ctgaccgtct accaaaatac gaccactgct gggtgaaatg atactgacta
    22381 gggattttat agtggtcgat tttccagccc cattatgacc aatcaagccc ataatctctc
    22441 cattttcaat ctgcaaattg agattactca aggcctcttt atcaccatac aacttactaa
    22501 cattttgaaa ttcaatcatg ggacaactcc tatctttatc tgtattatat atcgctatac
    22561 tatcactttg atacaaaaaa tcaatacttt attttaagta gttaaaacag tttccttcta
    22621 ttcttacgca aacgtttgcg ctaaccaata ctttatgata gaataaagaa caagattgac
    22681 aagtaagagg aaaaatcatg caaaatcaaa cgctcatgca atactttgaa tggtatctgc
    22741 cccacgacgg tcagcactgg acgcgtctgg ctgaagatgc tccacaccta gctcatctgg
    22801 ggattagcca cgtctggatg ccaccagctt ttaaggcaac caatgaaaaa gatgtcggct
    22861 atggtgtcta tgacttattt gacctaggag agtttaacca aaaagggact gtccgcacta
    22921 agtatggttt caaagaagac tatcttcaag ccattcaagc cctaaaagca cagggaattc
    22981 aacctatggc cgatgtggtt ctcaatcaca aggctgcagc cgatcacatg gaagcctttc
    23041 aggttatcga agtggatcct gtagaccgta cggttgaact tggagaaccc ttcaccatca
    23101 atggctggac tagctttacc ttctatggtc gccaagatac ctacaatgac ttccactggc
    23161 attggtacca cttcaccggt acagactacg atgcaaaacg ccgtaagtct gggatttatc
    23221 tcatccaagg ggacaacaag ggctgggcta acgaggaatt ggtcgataac gaaaacggaa
    23281 actacgacta cctcatgtat gccgacctag actttaaaca tcctgaagtc atccaaaaca
    23341 tctatgactg ggctgattgg ttcatggaaa cgactggtgt agctggtttc cgtttggatg
    23401 ccgttaagca tattgactct ttctttatgc gcaacttcat ccgcgatatg aaggaaaaat
    23461 acggtgacga tttctatgtt tttggtgaat tttggaaccc agacaaggaa gccaatctgg
    23521 actatctcga aaaaacggaa gaacactttg accttgtcga tgttcgtctc caccagaatc
    23581 tctttgaagc cagtcaagct ggcgcaaact atgaccttcg tggcattttc acagatagcc
    23641 tggttgaact caagcctgac aagtctgtga cttttgtcga caaccacgat acccaacgag
    23701 gacaagccct tgagtctacc gttgaagaat ggttcaagcc agcagcctat gccctcattt
    23761 tgttacgcca agacggcctt ccatgtgtct tttacggaga ctactatggg atttcaggcc
    23821 agtatgctca acaagatttc aaagaaatcc ttgaccgcct cctagccatc cgaaaagatt
    23881 tggcctatgg agaacaaaat gactactttg accatgctaa ctgtatcggt tgggtacgtt
    23941 caggtgctga aaatcaatcc ccaatcgcag tcttgatttc aaatgaccaa gaaaacagca
    24001 agtcaatgtt tgtcggtcaa gaatggacta atcaaacctt tgtagattta cttggtaacc
    24061 accaaggtca agttacaatt gatgaggaag gttatggaca attccctgtc tcagctagat
    24121 ccgtaagtgt ctgggcagtc aataccatct aatagctcat aataaccaag ctaggtccaa
    24181 gcggatttgg cttttttgta gtcaaaaaag acctacccaa atggatagat ctttacttga
    24241 ttacaattta cctgctacta catccaataa ttcttggatc ttagcttggt tgcttcctcc
    24301 tgccatggcc atgtctggtt taccaccacc acgtccatcg atgattggtg ctaattcttt
    24361 gacaaggttt cctgcatgaa ggtcttttgt cttgcttgct acaaggacat tgactttgtc
    24421 accgatagcg gcaactagga caagaagatc agagtagtct ttttgtttcc agttatctgc
    24481 aaaagtacga agggcaccgg catcggatac agacacttga ctagcaatgt aacgatgacc
    24541 gttgacttcc ttagcatctt tgaagatatc gcctgcggct gcagctgcgg ctttttcttt
    24601 caactcagca ttttcttttt gaagttgacg aagttgttct tgaagtcctt ctaccttgtg
    24661 aggtacttcc ttgacttgag gtgctttcaa ggttgctgcg acagctttaa gagcatcctc
    24721 ttgttcacga taggcttcaa aggcttcctt accagtcact gccaagatac ggcgagttcc
    24781 tgaaccgatt ccttcttctt tgacaatttt gaagagacca atctcagaag tgttgccaac
    24841 atgagtacca ccacaaagtt caatagagta gtcaccgata gtcacgacac gaacttcctt
    24901 gccgtatttc tcaccaaaga gggccatagc tcccatttct ttagcagtgt caatatccgt
    24961 ttcaactgtc ttcacttcaa gtgcttccca aattttctcg ttaacttgct gttcaatcgc
    25021 acgaagttcc tcagcagtta ctgcttggaa gtgggtaaag tcaaagcgaa ggaattcaac
    25081 ttcgttaaga gatcctgcct gtgttgcgtg gtttccaagg atattgtgaa gggcagcgtg
    25141 aagcaaatga gtcgcagtgt ggtttttcat gacacggtga cggcgattgc tatcaattac
    25201 caaggtatat tcttggttca aggcaagcgg tgcaaggact tcaactgtat gaagggcttg
    25261 accatttggg gctttctgaa cattggtcac agtagccaca accttacctg actcatccaa
    25321 gatttgtccg tagtcagcta cctgtccacc catttcagca taaaatgacg tttccgcaaa
    25381 gataagagag gcagttcctt ctgaaacagc tcctacttct gcattgtcag caacgatagc
    25441 taccaattta gaagacaatt ggctagcatt gtagttgaag acactttcta cagtgatgtt
    25501 ttgaagagtt tcattttgca tacccattga gccacccttg acagctgacg cacgcgcgcg
    25561 ttcttgctgt tctttcatgg ctgcttcaaa accttcacgg tctacagtca taccagcttc
    25621 ttcagcaatt tcttcagtca attcaactgg gaatccgtat gtatcataga gtttaaagac
    25681 atcttgacca gcaatgacag attgaccttt ttctttcaag tctgccacaa tgccttgggc
    25741 aaagtgttga cctgagtgaa gggtacgggc aaatgattct tcttcgctct taacgatttt
    25801 ctcaataaag tcacgtttct caagcacttc tgggtagtag ctttccatga tttttccaac
    25861 agttggaaca agtttgtaaa ggaaaggctc gttgataccc aatttttgac catgcataga
    25921 agcacgacgg agaagacgac gaagaacata accacgacct tcatttcctg gaagggcacc
    25981 atcaccgata gcaaatgaaa gagaacgaat gtggtctgcg ataaccttga agctcatgtt
    26041 gtcgccatct tggtcataaa ccttaccaga caatttctcg acttcacgga taatcggcat
    26101 gaagaggtcc gtttcaaagt tggtcttagc cccttggata acagccacca aacgctccaa
    26161 accagcgccc gtatcaatgt tcttatgtgg caattccttg tattcgctac gaggaacagc
    26221 agggtctgcg ttaaattgtg acaaaacgat gttccagatt tcaatataac ggtcgttttc
    26281 aatatcttct gcaagcaggc gaagaccgat attttctggg tcaaaggctt ccccacggtc
    26341 aaagaagatt tctgtatctg gtccagaagg tcccgcaccg atttcccaga agttgtcctc
    26401 aattggaatc aagtgacttg gatccactcc cacttcaatc cagcggttgt aagaatcttt
    26461 atcgtctgga tagtaggtca tgtaaagttt ttcagcaggg aaatcaaacc attcagggct
    26521 tgtcaaaagc tcataagccc aagtgatagc ttcgtcacgg aagtaatccc cgatagagaa
    26581 gttccccaac atttcaaaca tggtatggtg acgcgcagtc ttccctacgt tttcgatgtc
    26641 gttggtacgg atagcctttt gggcattggt aatacgtgga ttttcaggga taatggtccc
    26701 gtcaaagtat ttcttaaggg ttgctacccc agagttgatc cacaaaagag ttgggtcatt
    26761 tacaggaacc aaacttactg atggttctac tgagtgacct ttggtcgccc agaaatcaag
    26821 ccacatttgg cgtacttgtg cactagatag ttgtttcata ttgtctcctt atttacttgt
    26881 ttaatgtgat tggctttcca gcatttccac atagtcaatc gcgacacaga gggaaatgac
    26941 taggtctgca taagcgtctt caagaaccgt tacggtatag gtagaggtca gatggaagag
    27001 ttccttctta atttccgcaa tcaactgatc gcgatcatcc agcaatttga aattcaaatc
    27061 ccagatattg ccctcgatac gaagacctag attatcaaac tcatacttat ctcgccagaa
    27121 ggtcaacttc ttacgaatga caaaactcga gccatcccga agctgaatct caaaacgagg
    27181 aagcaaggtc aagatttctt tactaatctc actgacttgt tcaccagccg catcatagat
    27241 ggtaaaggtt ttaggaatct taaaaaatga tccctccacc tgataggcaa tttctcccct
    27301 gtcatccttg atagcgaagc gttcgcctcc aagacgaaac ttttgtttga caagaaatgt
    27361 tttcatcaac acctccaaaa atcaaaagac aagctcatat cacgaagggc gaaaaaccgc
    27421 ggtaccacct tcattcaatg aacttgtcat tctcttgttc ttatgcaatt gtatgattga
    27481 gtagcatgac ttcctagctt agatggctcg cagcaccgcc atttctctgg actaagacaa
    27541 gtgaaaatca attctcaact ttcttattat aacgtttttt taagcttgcg tcaactggaa
    27601 atgatctccg ttgaattaga ccaattccct acatctctga ttactttttc aggatatatt
    27661 ttttcttact gccatttttc tttttatccc aaattttcat attactaaac acagctacta
    27721 gaatatttcc aaatataaag gtgcctatca cccaatatat ggactcagtt gttaggtatt
    27781 gtcgatccaa gccatccttt aaatggaata gtataacagt ttggttaaca atcataaagg
    27841 ttggccagaa actttttttg aaaaaagtag acattttcat tatttgttgc cgctttctgt
    27901 aaggttaata ctcaataaaa atcaaaaagc aaactaggaa gctagcctca agctgtactt
    27961 gagtacggca aggcaacgct gacatggttt gaagagtata ggcttagtat actactaggc
    28021 aagcaaataa gcaaataaac aactagaata gaaaaagata gggctctaaa aactgacttc
    28081 tattccttaa aaacgaacca gcttgactga ttcgtcttct tacgtttatc tcctacttcc
    28141 gatacatttt aaactgtagg aagaggtcgc tatatttccc tgtccattta tggtcaaatt
    28201 tctcataaac ttctaggtgt ttcatggttt caacatcggg atagaaggcc ttatcttcct
    28261 ttgtttcctc tgggagcaat tccttcgctg gtaggtttgg tgttgaatag ccgacatact
    28321 ccgcattttg gagagcattt tcaggtttca acataaagtt gataaaggca taggctgagt
    28381 tttggttttt aactgttttg ggaatgacca tattgtcaaa ccaaagattg ctggcctctg
    28441 tcggtaccac ataacgtaga ttttcatttt tttctaacat ttggctggct tcaccagaga
    28501 aggtcacgcc gattgcaaca ttattctgaa tcatatagcc cttcatctcg tccgcaacga
    28561 tagccttgat atttggagtc agtttgtaga gcttatccac tgtctcttcc aactgctgca
    28621 gatccttgga gttgaggctg tagccgaggg aattgagtcc tagtcccagc acctcacgcg
    28681 ccccatcaaa gagcatgata gaattcttat actccggctt ccaaaggtca tcccaatgct
    28741 caggcgcttc atctaccatg gtttcgttgt agacaattcc taaggttccc cagaagtaag
    28801 ggatggagaa tttattacct gggtcaaagg actggttgag aaactctggt ccgatatttt
    28861 cgattccttc aatttttgaa taatcaagcg gaaccaagag gtcttcgtcc ttcatcttgt
    28921 taatcatgta ttcacttgga atggcaatat cgtaggtcgt tccaccctgc tttatcttag
    28981 tgtacatggc ttcgttggag tcaaaagtct cgtactgaac ttgaattcct gtttcttctg
    29041 taaactgagt caagagttca ggatcgatat agtctcccca gttatagata accaattttt
    29101 gactatctcg actattgatt ttactatcta aatgagtcgc aattccccac aagacaagga
    29161 taatcgctgc aattcctgct aaaaatgaat agattttttt catgcttgct cctccttctc
    29221 acgagagata aagtaataac ctacaactag gataatacta aagagaaaga ctagagcaga
    29281 cagggcattg atttctaagg aaatcccctt gcgagcacga gagtaaatct cgactgatag
    29341 ggttgaaaag ccatttcctg ttacaaagaa ggtcacggca aagtcatcta acgaataggt
    29401 gaaggccatg aaataaccag taatgataga cggagtcagg taaggaagca tgatttcctt
    29461 gaacatctga aattgactag ctcccaagtc ataggccgca tgaatcatgt cgccattcat
    29521 ttccttgagt cgaggcaaga ccatcaagac cacgatagga atggagaagg ccacgtgact
    29581 agatagaacg gtcaaaaagc caagtgaaaa cttgagttgg gtaaagagaa tcaagaagct
    29641 agcaccaatc ataacgtcag gcgcaaccat gaggatatta ttgagtgata gaaaggcttc
    29701 ttggtatttc ttacgagact ggtagatgta aatggcacca aaagtcccga taatggtcgc
    29761 tatcaaggct gataggaagg ccaagaaaaa ggtctgagtc acaattaaca taaggcgacc
    29821 atcaccaaac atggttttaa aatggctcaa gctaaagcct gtaaagctgt tcatgtcgtt
    29881 tcctgcatta aaggcgtagc caatcaagta aaaaatcggc aggtagagga caagaaagac
    29941 cagtcccaga taaaggttgg caaatttttt catcgttctc tcctttcctt ggtcacccac
    30001 atggtgatga acatggtcaa gatgagaatt acaccgatgg ttgaacccat accgtagttg
    30061 tcattggtta ggaaattctg ttcaatagcc gttcccagcg taataacacg gtttccacca
    30121 atcaaacggg tcagcatgaa gagactcaag ctggggataa agacagattg gaccccactt
    30181 ctcactccgt tcatagacag agggaagatg acatgacgga aagtctccca cttggtcgca
    30241 cctaggtcat agctggcatt gatgagattg ttatccatat cgtccaagac attgaaaatc
    30301 ggcaaaatca taaagggaag ctcgatgtag cttgcgacaa agataaagga gaaatcggta
    30361 aaaagcaact gctgtgaacc aattccgata aattccaaga attggttaat agagccattc
    30421 tgaccaaaaa tcccgataaa ggcataagcc ttaagaagaa ggttgatcca agttggcaga
    30481 ataatcagca taatccagag ttgacggtgt ttgagacggg tcaaaaagag ggccgtcgga
    30541 taactgataa gcagtgtcac aaaggtcaca atgcctgcat aaagcactga gttgaaactc
    30601 attttaagat aggtcaagtt ttgtgacgca aagtaagatt tgtaattttc taaactgaac
    30661 tggccttcga tgttgaaaaa ggattgaccg aaaatcaaga ccaagggtgc caatacaaag
    30721 agggcaatcc aaagcatgta gggcactaca aagagtttag agcttgtttt cttcatctct
    30781 ttcctcctcg attgcattga tcaaacctgc ttcttgctct tcgatttcta cgtactcctc
    30841 aatacgagca tcgaactctt cttcggtttc attgagacgc atgatgtgga tgtcttctgg
    30901 ttcaaagtcc agaccgattt cctcacccac gatagcctta cgggttgagt ggatcatcca
    30961 ttcatttcca agttcgtcat aggcgataat ttcataatga actccacgga aaagctgggt
    31021 atcgacctta acttggagct tgccttcttc aggaagggta atgcgcaagt cctctggacg
    31081 aataacgacc tcaacaggtt catttggctt catcccacca tcaaccgctt caaagcgttt
    31141 gccgttaaat tcgaccaagt agtcctcaat catgatacct ggcaagatgt ttgactcccc
    31201 gataaaggtg gcaacaaagt ggttgattgg ctcatcgtag atgtccacag gggttccaga
    31261 ctggacaatc tcgccatcat tcataacgaa aatccagtca ctcatggcca gggcttcttc
    31321 ctgatcgtga gtgacaaaga caaaggtaat gcccaatcgt tgttgtaatt cacgcagttc
    31381 gtactgcatg tctgttctta atttcaagtc cagcgctgac aagggctcat ccaacaaaac
    31441 cacacggggt tggttgatga tagcacgggc gatagccaca cgctgacgtt gtcctccaga
    31501 aagtttgcgg atggaacgtt tttcataacc ttccaactga accatcttga gaacttccgc
    31561 tacgcgttgc tcgatttctt tcttgtcaat cttgcgcaag cgaagtggaa aggcaacatt
    31621 ttcaaacaca ttcatatgtg gaaacaaggc ataggattgg aagaccgtat ggacatcacg
    31681 cttgttggtt ggaatatcat tgatacgaac accgtctagc atgatatctc ctgtcgtcgc
    31741 atccagtaaa cctgcaataa tgtttaggat agttgatttc cccgaaccag atgcacctag
    31801 aagggtgtag aatttccctt cttccaactc aaagttgatg tctttgagaa ccttggtgtt
    31861 gctgtcttca aaaactttag agacgttttt gaattcgata attggttttt tcaattggta
    31921 taaattcctt ctttttcata gattaacaga tcggggctct gtcaggtcgc cactacctct
    31981 tgcagggagt aaaaccacct gcatacatct tcgctaccga taggctttca ccccctttcc
    32041 atgacatagc agcagctttt caaatacagc ttcctacttg ctttcaccca agatccggac
    32101 ttctctttca agcgtaatac ctgagtgttc cttgactttt tcgataaccg attggatcaa
    32161 gtcctcgtag tctttggccg ttccatctgc gacattgatc ataaatcctg catgcttttc
    32221 tgacacttct acgccaccga tacgatagcc tttcaagcca gcttctgaaa ttaactgacc
    32281 tgcaaaatgc ccgactggac gcttaaagac cgagccacaa gatgggtatt ccaaaggttg
    32341 cttgagttca cgtaggtgcg tcaagcggtc catttcctgc ttgataacct gatgggttcc
    32401 tggagctagg gcaaatttaa ctgacaagac aactgcacca gactcctgaa tagctgaatg
    32461 gcggtaacca aaagccaagt ctttagcaga cagggtttcg atttctccat ccttggtcaa
    32521 gaccttacaa gactgcaaga tgtgagcaat ctcgccacca taggcacccg cattcataaa
    32581 gacagcaccg ccaacgcttc ctggaatacc acaagcaaac tcaaagccag ttaaactatg
    32641 acggagggca atgcgagttg tttcaatcaa gttagcccca gcttctgctt caatggtata
    32701 gccatcaaca gaaacgttat tgagcttgtc acacaagatg acaaatccac gaatcccacc
    32761 atcacgaacg atgatattgc ttgcattgcc aagaaccatc caagggatat tttcttggtt
    32821 ggcaaatttc acaaggcgcg ccaactcaaa acgatttcgt ggaaagacca aataatcagc
    32881 ctctccacct acttttgtat aactatagct atgcaagggt tccttaaaac ggatatcaat
    32941 tccttctaag atttcaagca ttttttctct tacagacatg tcactcttcc ttttacaaaa
    33001 ttcattccat tataccattt ttagagacat ttgacgacca taaaaatacc ttgtttggat
    33061 tttgcataag aaaaagaggt tccccccttt ttatgatttt ttacaaaaga tttccttggt
    33121 tccataggca accagaacga gatccagtgc tagaatcact tcaaccaaga ctggatttgt
    33181 caaccagcct acttggaaaa gagatggtgc cagatcaaag aaggcatgca agccataggc
    33241 tgctaggaga taaatccatt tcttctggcg aacagcttgg taaacccaaa ctgtcaaaag
    33301 taattggaaa ccaagcgcca agattcgctc aaaaccaagc aaataaatct gccagaccga
    33361 aagtgactga atggttttta acatattttc agacagtaat tgcataacct gtggattctg
    33421 agtttgaact gccgaaagaa cgatgtaaag attgagtaaa ctaataaggc ctagaaaaat
    33481 caactccaag ccaccatgcc ctaggccata ggccaaggca tctgcctttt ccaaacttct
    33541 ctttttctcc aaccatttga agaaaacaag acgagccgtt tcctcaaaaa atgctgccat
    33601 ggctaggcca tagataatat agataagcgg atgatcttgc aagagggcaa tactaccgtc
    33661 tttttgagga tgcaaaatca agatatgcac cagtttttcc aaaatctgcg aagagacaaa
    33721 gaaagcgact gcacccaagc ccaagacagc tagattaatc tggtatttct ttttggtaaa
    33781 ccaaatgctt cctatcagaa aagctagcaa cagcaacatg gtaataatga tatgaatggt
    33841 cattttcttc tcctattctg ccttttcaat atcttttttc atctcgtcaa cattgaactt
    33901 agcaaacaag tattgacggt cttggactgg gaaacgttgg tccaactggt caactgctcc
    33961 cacctcgata atgccttcct tgatgacaaa atccatgaca tgtccaccga aggtcaaatc
    34021 atctgatatg aagtgcagat ggtagcctgc cacactgacc ccatggaaaa tctgaggcgt
    34081 ccagaaacca acaatagtcc ccgcaacatt gtcacgacta tattccggtt gatgggttgc
    34141 gatatcagca aacttggtat cgggtgttga cttaggaatc atacgcacat gcatatgtga
    34201 aaattccccc cgaatcttga tagagcggaa aagattttcc ccatcataat aagactcaat
    34261 tcgttctttc aattccttgt ctgtcatctc aaagcgctgg cggaaaatga cctctgcctg
    34321 atgcggtacc actgcagcgt aaggaataag ggcatctggt gacacttcca caatttctgg
    34381 ctggtctcct gactctttgg cctgataagc cttgccatcc aagacaatca attccccatc
    34441 aatggaatcc aaggttccca aaccaaggtc accatgttct agcaattctc ctactgtcat
    34501 agtaccacca taaaggccag ccatcaaggc tccaagagta ttgtattgaa ataatttcac
    34561 tggttcctgc acgttcttat ccattcactt tcttgtctgt tattctataa atcttactcc
    34621 taagtatacc acatttgccc ctagatgtga acgagagaaa cgctctagac attgccaaga
    34681 aggaaaaaaa agggtacaat gtaacaaaat caagggaggt ctggaatgaa gaaacaaagc
    34741 aagtacaaag aggtcgtttc ctatctgaaa aatggtatcg agtctggacg atttccgacg
    34801 ggtagtcgcc tgccttctat ccgtcaactg agccttgact ttcactgcag caaggacacc
    34861 attcaacgag ccctgctgga attacggcac gaacaatacc tctatgccaa gcctcagagt
    34921 ggctactatg tattagaaca agggcaacat caagacctag aaatcgaggt taccgacgaa
    34981 catgccagtg cctatgacga tttccgactc tgtgtcaatg aaaccttgat tggccgagaa
    35041 aactacctct tcaactacta tgacaatcaa gaaggattag aagacctaag acaatccatt
    35101 cacaaactcc tctttgacca agctctctac tgcaaggcta accaactagt actgacttct
    35161 ggaacccaac aagccttgtt tatcctctct caaatatcct ttcctagcca agccaaggaa
    35221 atcttggtgg aacagccaac ctaccatcgg atgaatcgcc tcttgattgc acaggggctg
    35281 gactatcaaa cgattgaacg aggcattgat gggattgact tggaggagct ggaaggccac
    35341 ttcaaaacag gaaaaattaa gtttttctac accattcctc gatttcacta tcccctaggc
    35401 cattcctatt ctgagcagga caaacgagct attcttgact tagcagccaa atatagtgtt
    35461 tatatcgtcg aagacgacta tctgggggat ttggactcta aaaagggcca gaccttccac
    35521 tatctagata cagaggaacg cgtcatttat atcaaatcct tctcaaccag cctctttcca
    35581 gccttgcgga taacagcact cattcttcca aatactatca aagaagcctt cgtggcctac
    35641 aaaaatatcc tagactacga cagcaacctc atcatgcaaa aggccctgtc actctatatt
    35701 gacagtcaat tgtttgaaaa aaatcgtttg gctcgcttga ccaatcatga atcttaccaa
    35761 aaacaaatcg aggaaaggat aactaaaaca ccttgtcccc ttcctcatta tcccctacac
    35821 gatggtttat tgctagacct gagacagtat cctaaaatcg ccagtctcaa acacagtcaa
    35881 ctgggcttgg acttctttga agaggcctat ttaagcacct gtccttatca atttgccaag
    35941 gtgtccctag acaatctgga aaatgtttta aactatttaa aagcagaatt ggaatgacat
    36001 ccaactctgc tttttcttat tcttcaactt cagttaaact tgctgctttt tcaagatagc
    36061 tttggtaagt tccttcatcc ttaagttttt ggatgacctt gtctaccact tctttcaaat
    36121 catcactatt ttttctaaga gcaacgacat tggcgtcgcc gtccttcatc ttcaagctga
    36181 cagttgcgac agctaagcca gcgtttttag cagcataact aagtgcaaca ggctcatcca
    36241 tatgaacagc atctattttt ccagcctgca attcattgac tgcttcaccc atattagtta
    36301 gggaagttaa ttgagctttt ggcaattgtt ccttgaccat tgattctgga acagtccctt
    36361 tttgggctgc aatattagca ctttctaggc tagttaaatc cttgtatttt tccacatcag
    36421 ccttacgaac caagaaacta atcttgtttt catagtatgg gattgaaaaa tcaaagactt
    36481 cttttctctc gtcagtagca ctaattcctg caactgctag gtcagcctta ccagtttgaa
    36541 gactggtcaa aacattgtca aaactcatgc ttaagatttc caacttaacc ccaagttcat
    36601 cagcgatagc ctgagccatg tcgatgtctg caccgactac ctggttcttt ccatcaacca
    36661 atgattgaaa ttcaaagggt gcatagtcag gactggttgc cacaactaat ttcccctttt
    36721 gcttaatggc ctcaacagct gactgagaac cattagaacc tgactggcaa gctactaaga
    36781 agaaacttgc aagaaaatta cataaaacaa atatccattt tttgatttca taaataaaca
    36841 acctttctgt taattttgta taattataac gatatccaag ttacttgtca agtgtttttt
    36901 aaatttttat ctcaaaaata ttttttcgtt caaaaaaagg agccatcagt tgatttcaag
    36961 ctccttttta tacagaatta aactatttta tagttcgaca atcttacctg tttcaaagta
    37021 gacaacccat tcacagatat ttttagcata atcaccgata cgctccaagt aggaaataac
    37081 ttggaaataa tcacgacccg taacaatggc ttctggattt ttcttaatct cttcagtcgc
    37141 aaggtcacgg atagtttcaa aatagtggtt aatttgctca tccatggagg ccacccggta
    37201 tgcgtcgtca acagaaccat taagataaag atcaagtgct gcttcaacaa cgcttttaac
    37261 ttcacgtccc atttttttaa tttcttcctc tacagctggg atgcgttctt cccccttcat
    37321 acggatggtt gcctgggcaa tggctacagc gtgatccccc atacgctcca catctgatac
    37381 agccttaagg acagtcaaga ctgtacgcaa atcttgagag actggttgtt ggagtgcgat
    37441 catttcaaat gatttctttt ccagtttcac ttcgtattca tttacttctg catcatcttc
    37501 tatgacctct tttgccaggt cacggtcatg cgtgacaaaa gcacgtaccg tacgattgat
    37561 ttgtgagagc acttcttgtc ccatagcgta gaactggtta tgtaatttct ctaaatcttc
    37621 ttcaaattga gatcgtaaca tctttcatct ccttatccaa attttcctgt aatatagtct
    37681 tccgtttcct tgtgttgggg atcaaggaac atctgcttgg tatcattaaa ttcaatcaaa
    37741 tctccatcta ggaaaaatcc tgtcttatca gagatacgtg aagcttgctg catggaacgg
    37801 gttaccagaa gcatggtgta cttgtctttt agaccataca aggtttcctc aattttacca
    37861 gctgaaatcg gatccaaagc cgaagttggc tcatccaaga ggatgatttt aggactagtt
    37921 gccaagacac gggccacgca gacacgctgc tgttgaccac ctgacaatcc aatagctgaa
    37981 tcatatagac gatccttgac ctcatcccag atagaggcac cttgcaaggc tttttctacg
    38041 gcttcatcca gaacctgctt atccttaatt ccattgatac gaagcccgta gacaacattc
    38101 tcatagatag tcatagggaa aggattaggt tgttggaaaa ccattccgat ttccttacgt
    38161 aattcaaccg tatctgtacg cggactgtag atgttgtgac cattgtacac cacggatcca
    38221 gttgtggtca cctctggatt gagatctccc atgcggttga gagacttgag gagggttgac
    38281 ttccctgatc cagatggacc aatcaaggct gtaatttcct taggttggaa agatagggaa
    38341 acactattca aagccttctt tttattataa taaacggaca ggtctgatac ctgtaaaatc
    38401 gcatctgtca tacggtttcc tttctaacca aagtgaccag atacatagtc attggtggac
    38461 tgtagcttgg cattttggaa aatagttgca gtcttatcat actcaatcaa atcacccaag
    38521 taaaagaagc ctgtatagtc acttgcacga gcagcctgct gcatattatg cgttacaatg
    38581 atgatggtaa agtttttctt gagctcaaac atggtctctt ctagttgcat ggtcgcaatc
    38641 ggatccaagg ctgaggctgg ctcatccatt aagaggatat ctggcttaac agagatggca
    38701 cgagcgatac agagacgttg ttgctgacca cctgataagg tcaaggctga cttgtggaga
    38761 tcgtctttaa cctgatccca gagggcagcc tgacgaaggg aggtttctac gatttcatct
    38821 aggacttgct tatccttaac tccagcacgt tcatgcgcaa aggtgatatt acggtaaatt
    38881 gacttagcaa atggattggg gcgttgaaaa accattccaa tgtgtttacg catttcataa
    38941 acgttgattt ctggacggtt gacatcaatt ccacgataga gaatctgccc agttacttta
    39001 gcaatatcaa tagtatcatt catgcgattg agactgcgta agtaggtaga tttccccgac
    39061 cccgacggac caatcaaagc tgtaatttta tttctttcaa attgcatatc aatcccctta
    39121 atggattcat ttttaccata gtaaacatgg acatccttag tagaaagggc tactttttct
    39181 tcaggaaagg taaggatatg cttctcatcc cagttatatg ttgacatggc ttctccttta
    39241 ggcagcggtt aatttcttgt gtagatagct tccgaactta cgagctccaa agttaaaaat
    39301 caggataaag atcaggagca cagcggcaga acctgctgat acaatggttc catctggaat
    39361 agtgccttca ctattgactt tccagatatg gacagccaag gtttctgctt gacggaagat
    39421 agagatgggg ctagtcacac tgaggatatt ccagttagac cagtcaagag ctggcgccga
    39481 ttgccctgct gtatagatca gagctgcagc ttcgccaaag atacgaccag atgccaagac
    39541 gacacccgtt acaatacctg gaagcgcttc tggaataaca acatgaacca ctgtctccca
    39601 gcgagaaatc ccaagagcca gaccagcctc acgttgggta tggtgaacgt gtttcaaact
    39661 atcctctaca ttacgcgtca tctgaggcaa attaaagact gtcaaggcca aggcacctga
    39721 aatgattgaa aatccatact caaactggac tacaaagatc aagtaaccaa agagacccac
    39781 caccactgat ggtaaagagg acaagatttc aatacaagtc cgcacaaagt tggtaacagg
    39841 acctttttta gcatattcag ccaagtaaat cccagctccc atagaaagag gtacagaaat
    39901 aatcaaggta atgaccaata ggaaaaagga attgtaaagc tgaatgccaa tcccaccacc
    39961 tgcttgaaaa gcagaagacc ttccagtcaa gaaagaccaa gagatatggg gcaagccccg
    40021 aaccaagata tagagaatca aggaagccaa gattgtcaca atgatgctag caatcgtata
    40081 gaggacagct gttgcaagtt tatctaattt cttagcgcgc ataatttttc tttcctcttt
    40141 ctttcgtaat caatttaatc acactgttaa aaactaaact catcaagagc agtaccaagg
    40201 ccagtgacca gagaacatta ttatttacag ttcccatgac agtgttccca attcccatag
    40261 ttaatataga agttaaagtt gcagctggtg tggtcaagga agttgggata acagctgagt
    40321 ttccgacaac catctggata gctaaagcct caccaaaggc acgcgccatc ccaaagacca
    40381 ctgcagtgaa aataccagaa cgggccgcct tcaagatcac acgccagata gtctgccagc
    40441 gagtggctcc catagcgaaa cttgcctcac gataataacg aggaaccgca cgcaagctat
    40501 ccgttgtcat aaaggttacg gtcggcaaaa tcatgacaaa gaggacggaa atccctgaca
    40561 aaatcccaaa accagtccca ccaaagacac tgcgaacaaa gggaacgacg acttgcaagc
    40621 caataaatcc gtacactact gaaggaatcc caaccaggag ttcaatagct ggttgcaaaa
    40681 tcttcgcccc ttttggtgat acttcggtca taaaaactgc tgcaccaata gcaaagggtg
    40741 ttgcgataag ggctgagaga atggtaacga taaaggaacc caaaatcata ggaagggcac
    40801 caaattcttt actagaagga ttccaagttc ctcccaaaag aaagtcaaag atattcacac
    40861 cattgacaaa gaaggtcgac aagccttttt gcgctacgaa aaccaaaatc atggccacaa
    40921 ggatgactat caaagaaaga caggcaaagg tcaaaccttt tcctaatttc tccagacgag
    40981 aattctttga tggaagcaac attttcttag ctaattcttc ttgattcatt attgtctccc
    41041 ttccaacact gtcacagttc cggcagcatc tttttcaacc ttcatttcct taatcggaat
    41101 atacttcaat cctttgacaa tcccttcttg ggtctcatcc gagagaacaa aattgagaaa
    41161 ttctgcagct aactcattgg gctgccccaa tgtatacata tgctcataag accacaaggg
    41221 ccaattattg ctacttatat tttctggact taagtcatag ccattcaact tcatgctttt
    41281 gaccgaatca tctatatagg taagagataa ataagagata gctcctggac tttttgatac
    41341 gattgatttt accgctccat ttgaatcctg ctcctgactt tgcatggcag actgaccttc
    41401 cataatgaca gtatcaaagg tagcacgaga gccagagccg gctgcccgat tgataacaga
    41461 gatgggtaag tccttaccac caacctcttt ccaattggtt acctcaccta tgaagatttg
    41521 acgaagttgc tctgtcgtta gattatcaac atcaacctcc ttattgacaa tcagagccaa
    41581 gccagctacc gcgaccttgt ggtcaacaag agcagaagca tcaattccgt ctttttcctc
    41641 agcaaataca tctgagtttc ctatatcaac tgccccagac tgaacctggg acaagcctgt
    41701 accagaacct cccccttgga cattgaccgt ttttccaaca tggatcgtgc caaattcatc
    41761 tgccgctact tcaaccaagg gttgcaaggc agttgagcca acagccgtta tggattctcc
    41821 acgatcaatc cagctagcac agcctactaa acaagccgtc agccaaaaag cgataagaga
    41881 cagagcaagc ttttttcttt ttttcactgt ttttctcctc gaaaataatt atgaatactg
    41941 tgaatttttt aagtagttct ttatgagttg acgcatgaat tcttaccaaa tttctgcgca
    42001 attgattatt tatataatat aggctatatt actctttcct aacctccttt tttcatatgt
    42061 ggataaaatc tcttgtctat cccttccccc attgtcaccc attatagtca tttcgtgtct
    42121 ctttttcccc tttttaatgc aagggaaatt actctcctta gatgataatc taaaagctag
    42181 aaaggtatct caaatctctc tactctccca gactagttta caactaaaag gaaaagattc
    42241 tattttatga gaaatctagt ttacaagcgg taagaacgct aataactaaa cttcttgtac
    42301 tctttgaaaa tctcttcaaa gcagtgtttt gagctatcta tggctagctt cctagtttgc
    42361 tctttgattt tcatcgagta gtaaaactac atgtgatggc aatcaagata tcaagaatca
    42421 tcctactaaa aaaatccata ctttcactat aacatagaat aagatatttg actagcattt
    42481 tcatttgaat ctgaggcctt ttggaaaata atttttcaaa acatttccag taacctttgc
    42541 aaagcccaag ccattgcctt taaccaaaac ttggtaccaa ccatttggca gactttctgc
    42601 cagctgaacg gtttctccag ccgcatactt gacaaactct tcttggccaa tttcaaccga
    42661 ctgttcgacc tgactcggtt tcaaggctaa accaagagcg aaactgggct caaagcgttt
    42721 cttcttaaaa gtacccagat gcagtccatt gcgagcaatc ttgagcttcc ctaaatctgg
    42781 caaaagttct ggcaagagat aaagctggtc tccaaaaatc tgcaagatac ccggtagatt
    42841 gaccttcaaa tggttttggg caaattcctg ccacaaggca acttgttcac ggctgaggtt
    42901 actcttactt gccttaaatt taggagctgg attgttaccc ttaaactgta gatgggcaac
    42961 aaactgaccc tctcccttaa actgatgagg gtacatccga gccgtttctg gcaggtcaat
    43021 accagctacc attccattga tatgctctac tggcaacaag tcaaaatcat actcttccag
    43081 caaccaattg acaatctctt cgttttcctc gggtgcccag gtacaggtcg aataaaccag
    43141 acgaccacct tcagctaaca tggtcactgc atcctccaga atttctcttt gcaagctagc
    43201 acattgactc ggataatcta agctccaata gtccatagca tcaggttgct tacgaaacat
    43261 tccttcacca gagcaagggg catcaagaac gattaagtca aaatagcctt taaagacctt
    43321 gaccaagcgg tcggcagatt cattggtcac cacgacattt gtcgctccaa aacgctccat
    43381 gttttcaacc aaaatcttag cccgtttgct tgaaatttca ttggaaacaa gtagcccctc
    43441 ccctgctaga taggctgcca gttgagttga tttgcccccc ggtgcagcag ccaagtccaa
    43501 gaccttcata ccaggactgg gttgggctac ttgagccacc atttgagcag caggttcttg
    43561 cgaataaact aaacctgtag catgctcagg cgatttccct gaaaccttcc catagtggcc
    43621 ccaaggggtt tgagtaatgg catcagaaaa ggaaagttgc tcttctttta agggattgac
    43681 ccgaaaggcc gaaaccgctt cctcctcaaa agaggcaaga aaatctcttg cctcatctcc
    43741 tagtatttct ttatattttt caacaaatcc ttctggaaat ttcatttaag ttcttttcct
    43801 ttcgtaaata taggactgaa tttcctcctg catctcaaga ggcaccatca tgaccggctg
    43861 tctggtttga aaatcaggag cttcaccaaa aagggtcaca acccgatagc ccagactttc
    43921 ccctaaaata ctagctgcgg cataatccca tggttgcaga taggtgagat aggtcaacaa
    43981 acgccctgac aaaatcttgg caaaactaat ggccgcactt ccatagacac gaacaccaag
    44041 aaccgctcgg ctcaaatcag ccagccccca ttcattggtt tccagcatac cactattccc
    44101 tgcaatgaga aaatctccaa gtggtttagt tttaaaagaa gctaggggcc tatcatttag
    44161 acaaactgga aattccccac caccgtggta acaatcccct ttgaccacat cataaatcag
    44221 accaaactgt ccctgaccat tttcaaaata agccatcata acagcaaaat cttcctgctg
    44281 ggctacaaaa ttattggtac catcaatggg atcaatgacc caaaccttgc cctcttgaac
    44341 cgaggctcgc agacaacctt cttcagcaca aatcttatcc tcaggataac gggacaaaat
    44401 ctcaccaacc aagagttcct gaacttcttt gtccagtctg gtcaccaaat ctgttggaga
    44461 ggacttggtt tcaacacgca agtcttcctg catatggtca agaatgtact gacctgcttt
    44521 cttaacaagc tctttagcaa attcaaattt actttccaag agaaatcttt ccttcccctt
    44581 tttctttggc agcctgaact gctcggtaaa gtgaatagcc actaaccgtt tcaaattctc
    44641 gtcccaaacg tttctcttcg ctcttgcttg gcacgaccgc tttgaatccc ttataggagt
    44701 ccagtaactt tttagcctct actttgcctt cataggcagc ttcaacatca ttaaaaaaag
    44761 aaagcactga agcaagttct tcagtgctcc acgacaaatc tagtgggtaa ctatactgtt
    44821 tgttcattaa ctaataccag ctctcattct tgcttctttt agttcttgct tacgataact
    44881 acgagggaga aaagcacgaa tctcatcttc attaaaaccg atttgcatac gcttggcatc
    44941 aataataatt ggacgacgca aaagactagg atactgctca atcaaatgaa gcaattccga
    45001 taccgaaata ctctctacat caatattcaa tttttgaaaa atttttgaac gagttgaaat
    45061 gatgtcatca gtaccatttt cggtcaagga aaggatttgt tgcaattctt ttcttgttaa
    45121 aggactggtc ataatattgt gttccacaaa gggaacctta tgtttttcta accaggcctt
    45181 agccttacga catgatgtac agctcggtga tagaaatagt gtaatcatgc ttttctcttc
    45241 ttatctatac tttgctactt ctattataca aaaaaataag gcgcttgact agggattttt
    45301 agaaaaaaaa gcctattttt caagaaaaat aggctgtttg cgaacgattg acacaattgg
    45361 atttggttaa ttcactctta acgatggttt taaacgatat atatttttat atatgcaaat
    45421 taaaaacatc tttcatttca tttcctacga cttttcagac acagatagcc aaagaagctt
    45481 tcatagaggg caaaaaagag gaggaaggca tgaagaaaga aggtctctgg caaaatcata
    45541 ataacaggat ccttggctgg atcaaaaagc caggtatcat ctcccacaaa gagaatttga
    45601 tggaaaagag taaagaattg gtcaaaacca atcaaaactc ccccaagtcc aatcatcaca
    45661 ggtaagacta ctagagtcag gagacttttt cgataaagag acaaaaagtc ctttttcaca
    45721 attctattga caaagacata gaaacttggc agtgtcacta gagctactag ctgaaccaaa
    45781 tgaaagagat tcttgaccac tgcgaaatgg tgcagaccag ctgctgacga acgaaaatca
    45841 ggcatctgta agacctgact aaaaggattg gtcagataat tcatcaagat atgaaaattg
    45901 tattgaatgg tttctggttt tagatagact cgattcgtta agtttagcca ctgaatctcc
    45961 ataggataga aaatccaagc cagataaatg gtcagaagga ttgagaggga gaggagaaag
    46021 agcatagagc cccaaaagat caatttagtt ttcatcaaaa tcccactccg caaggctaga
    46081 aaccacatgt gtcggtgcga ttggcaggcc agctacttct tctgccttag taaaacctgt
    46141 cgtcaccaag agcgttggaa tgccattgtc aatcccagcc cggatatcag tcaaataatt
    46201 gtccccaacc ataattaact cttcacgttc caaacctaag tgctcaaccg ccttgtccat
    46261 aatgatggca ttgggttttc cgatataaac cggcttcact cgtgtcgcta cttcaagcag
    46321 cgtaatcagg gagccagcac ctggcaaaag accacgttcc gtcgggatgt tgaggtcagg
    46381 attggttccg ataaaatggg cacccttttg aatagcaaga gttgctgtgg caaatttttc
    46441 atagtcgact tgccaatcca gaccaactac cacgtaggca gggttttcct tgtcttccac
    46501 ataaccagcc gccttgatgg cttccttgag tcctgcttct ccgacgacat agacggtctt
    46561 ttcaagcccc aaatcattca tatagtcgat ggttgccaaa gtcgctgtgt agacagtcga
    46621 taggggcgta tcgatattaa aattctgagc caacatctcc ttaacactct ctggagtgcg
    46681 agttgtattg ttggttacaa agagataggg aatgtcccgc ttttgcaatt catgaacaaa
    46741 agtctctcca gcagggattc ggtctttccc cttataaatg gttccgtcta aatcaattaa
    46801 atagccttta tatttcatct atttctccct aagccttttt tatttctttc caagtaatga
    46861 ttgcttgggc attgataacc ccatcacttg taatttcatg cttgctttcc agtccagtcc
    46921 gttcaacagc cgatgtaatc accccacctg gtcgaacttc cttgacatac ttgaggttga
    46981 ttttcttggg aatatagtgg gtcaaaaaat ccgctcccat gacctcaaaa atccagtcca
    47041 agtatttact gttattgaca tgaccattca tatccaagtc gtaaaaacga acatggtaat
    47101 ccttgctgat cggctcttcc aaggactcat acttcggtcc acggataagt tttttatcaa
    47161 aatcagactg gtaaggagcc acaatctcag gttcaacagc atggactttt cgactgtcgc
    47221 ggtccatgag aacaaaggtc gccatcatat ggatgagctc ctgccccgct tcattataaa
    47281 tagtaaagcg acggtagcaa aaaagtcgat tgtagctcaa ggcttccgtt tcgatggtaa
    47341 tttcttccgc aaaacgaggc aaacgaacca cctcaatatc atattctgcg ataatccaga
    47401 ccagattata gtcttccaaa atggccttat cactaactcc cagttcaatc gactgcatcc
    47461 ctgaaacttg cagtgacagc aaaatcacat ctggaagttt gatatgaccg ttcatatcag
    47521 ccatatcaaa aggaattttc attttcattt gataagttaa gcccatgatc ctactccaaa
    47581 ataaatcgtt ctgctacagt atctcccaaa aagagacctc tctttgtcat gcgaacgtgg
    47641 tcaccctcaa tctgcatgag gccttgttga accaaatctc tgacaatttc tccataaagt
    47701 ccagcaaaag actgtccaaa tttttcctca aatcgcgcca tggaaacccc ggatttcttg
    47761 cggagtccca agaacatttc ttcttccatt tgctcctttt gactcaggtg atcttctgta
    47821 atacaagcat tgccttcctc aaccgcactg agataatgac gaatgggacc atgattttta
    47881 taacgtactc cattgacata accagatgcc cctgcaccaa taccatagta ttcagcattg
    47941 tcccagtaca tgagattgtg acgactttca aagccgggtt tggagaaatt agaaatctca
    48001 taatgctcaa aacctgctcg ctccagctct gcaatgatgt actcaaacat ctccgcttct
    48061 agttcctcct taggcagagg caatttccca cgtcgcatcc ggttcataaa gaccgtatgg
    48121 ttttctaaaa tcaaactata caaactcatg tggggaatat ccagtccaat ggctttagcc
    48181 acattttcct ttacttgctc catggtctga ccaggcagag cataaatcaa atcaatggag
    48241 atattgtcaa aaccagccag tttcaggcga tcgatatttt cataaatatc cttctccaaa
    48301 tgactgcgcc caattttttt caacatctta tcatcaaagg tctgaacacc tagcgaaaca
    48361 cgattgacag ccgaattttt caaaacagct atcttatccg catccaaatc gcctggattg
    48421 gcttcaatgg tcaactcttc caagacagac aagttcaagt ttttagtcaa gccattcagt
    48481 aacacctcca gttgcggagc cgacagggct gtcggtgttc caccaccgat ataaagggtt
    48541 gacaactttt caatatcata agaacgaaac tcttccagca gatgctctaa atagctgtcg
    48601 actggctgat ttttgatgaa gacctttgaa aaatcacaat aataacaaat ctgggtacaa
    48661 aatgggatgt gcacataggc tgacgttggt tttttctgca tagtaattat tataccacaa
    48721 agactagatt ccagataaaa atcaccatcc ccagatacat agtccgtccg gagatggtga
    48781 tggtttattc ttctgttata tcaatcacaa tctcttctga gtcatcaaga gcttcggctt
    48841 tttcttgcca ttgctccttg agattattta attgattttt tgatgcttct gtcgcttgaa
    48901 aagcatagga tttagcttga gcaagtatac tgtccacagt gatttcacct gactcaacct
    48961 gttcttttgt tttcagaaca aaatctgtag cctgctcctt aacttctgtc agtttttcac
    49021 agacttgctc cttggcatac tccggatctt ctctcaaatc atctagaaaa tcttgagcct
    49081 gactgcaaac ttgtttgccc ttatcacttg ttaaaaacaa ggcaagagct gcacctgaaa
    49141 ccgttcctaa aaggattgag gataatttac ccataaggat tctccttttt tattttttga
    49201 aaaatttact tgcaagacga agagctgaca gacttgcacc agtcttgagt gtttttgaac
    49261 cagctgatga agctttcttg ctcaagacac gcgcatggtc attgaggtct gaaacagata
    49321 gagataaatc tgcaacagca ctgaagagtg gatcaatcgt agccaccttg acattgatat
    49381 catctgccaa gacattgacc ttagccaaca actcattggt atgatgcaag gtcacatcca
    49441 catctgaagt caaggtttta atcgtctttt ctgtttcatc gatgacacga ccaagctttt
    49501 gtacagtaat gatcagatag accaaaaaga caatcaaagc tagggcaaca agaatatatg
    49561 caacttctaa catttagttt tcctcctctg taatatagta aggggccttc tttcgatttt
    49621 gataaataac gatcattata ccgagaccga taaggacaac tgacagccat tgggacactc
    49681 gaaggccgaa gaacatgaga ctatctgttc gcataccttc gatgaccata cgaccgaaac
    49741 cataccaaat caagtaaaag gccgtgatat gacctcgtct gagactcttc catttccgtc
    49801 taaaaatcag aatcaaggca aagccaagca gattccatag agactcataa aggaaagtcg
    49861 gttgacggta gctcccctca atatacatct ggtcacggat aaagccaggt agataatcaa
    49921 gattatccac tgttgcacca taagcttctt ggttaaagaa attaccccaa cgccccaaac
    49981 tttgagcaat cataacgcta ggcgccgcaa tatctagaaa atcccaagta ttgatgagtt
    50041 tacggtcagc aaagatatag agcacaagag ccccagttat caaaccaccg taaatggcca
    50101 aaccaccatt ccaaatggca aaaatctctc ctaaattctg actatagtaa tcaaatcgga
    50161 aaataacata gtagagacga gctcctaaaa tagccaaggg aaaggctact aagataaaat
    50221 ctaaaatatc gtctggtatg atcttctttc taggtgcttc tttcatggtc aaataaaccg
    50281 caagaatcaa gcctgtcaca atacataagg cataccaacg aatggctagg ggtcctagtt
    50341 gaatagcaat tggatcaagc attttgcacc tcatttcgag cgattagact tgtcagtcgt
    50401 tcgtcgaaca aacgggtcgc atcaaagccc atttccttgg cacgataatt catggcagct
    50461 gcctcaatca caacagagat attacgacct gttttaactg gaatacgaat acgaggaatg
    50521 gctacgccag aaacttcaag ttcctctgca ttgtttccaa gacgatcaaa ggtcttatgt
    50581 gtatcgtaat tttccaaata gacagcaagc tgaacctgtg aagaatcctt gacagcactc
    50641 gcaccgtaga gactcataac atcgataata ccaaccccac gaatttcaat caagtgtttc
    50701 aaaatttcag ctggttcacc ccagagagta atctcatcct tggcaaagat atcgacacgg
    50761 tcatcggcta ccaaacggtg accacgtttg acaagctcaa gacctgtctc gctcttacca
    50821 attccactat ctccctgaat caagacgccc atcccataaa tatccatcaa gacaccgtgc
    50881 acactggtac gttctgccaa acgagaatcc agatagctag ataactctcc agataaacga
    50941 ctggtagctg cacggctggt taaaatagca atcttacatt ctctagcagc ctttaacatc
    51001 tcctctggaa ccaccaaacc acgggcaaca atgactgctg gtgtctcagg tagaaacatt
    51061 tttttcaaaa cttcataacg gctgttggaa ggcatgctga tcagataaga ccactccttc
    51121 atccccaaaa gttgaatccg ctctggtgta tagtagtcaa aatagcctgt catttcaaga
    51181 ccaggtcgcg taatatccgc tatattgatt tccttttcaa gcaattctgg ttcaccatag
    51241 acaatgtcta gtctaagctt ttcaatcact tcttttacta aaaccgacat catttcctcc
    51301 ttcaaatgag ttcttctctc tattatatca aattgtagga ggaagtttca gatttttgca
    51361 ggctttggag tatttattta aaagaaaaag gactagattt ctctagcctt tcattccaat
    51421 tagaattttt tacgtttacg agctacttct gatttacgtt tacgttttac agaaggtttt
    51481 tcatagaatt cacgtttgcg tgtttcttga agagtaccag ctttagtaac cgcacgtttg
    51541 aaacggcgaa gtgcgtcgtc aagagattca ttcttacgta ctactgtttt agacattttt
    51601 ttcactccct tcaagttcaa tatctatttc attctacacg taaaaggaat aattgtcaag
    51661 caaaaatgcg gtttaatttt gattttttct tccatttata caagagttga aagcgttcaa
    51721 tagaacattg cttttttatt tttcgagtaa gctaagcgct tcagcatctg cgatgatggt
    51781 tacatcaggg tgattttgga ggctacttgc aggtaggttc tcagtcactg ggccagatac
    51841 tgttccggca atggcttctg ctttcgactc accgtaagca aaaagaataa tagacttggc
    51901 atccaaaatg tttttaatcc ccattgaaat agcttgggtt gggacgtctt caatcttggc
    51961 aaagaagcgt gcattggctt caatagtaga ctggtcaagt tctactagat gcgtttgact
    52021 gtcaaatgga gtaccaggct cattaaagcc gatatgccca ttgcgaccaa ttcccaagat
    52081 ttggaggtca actggatggt cagccaaaat ttggttgtag cgttcaactt cagcttcagc
    52141 attgtcctta acaccacgag gcaagaaact ttctttaaat ggtttttggt tgaacaagtt
    52201 ttcttgcatg aagtaacgat aagactgtgg attgtcccca tcaaggccca catactcatc
    52261 aaggttgaca ctggttagat ttgaaaaatc aaggttactc tcaacaattt ccttgtaaaa
    52321 ttcaagtggg ctacttcctg tcgcaagtcc tagagtttga gctccattag ccaatttttc
    52381 cttcaacatc tcaaaagcaa cttttccacc ttgaacttgg ttttcaactt taataacttt
    52441 catcttttca tcctccattt ctgtctatat ttattatatg gtatagacca atttttgtca
    52501 agtgaaaacg atttaatttc taaaaaaaga gcgtccaaac ttgaaacgtg ttataatgga
    52561 taggattaga acttagaaag aatgaacaga tatatgaata cagctgattt tgatttccac
    52621 ttgccagagg aattgattgc ccaaacgccc cttgaaaaac gtgatgcctc caaactcctc
    52681 atcgtcaacc gtgagacagg agaaatgcaa gataaacact tccactctat tattgatatg
    52741 ctggaacctg gtgatgccct tgtcatgaac gacacccgag ttctccctgc ccgcctctat
    52801 ggtcaaaaag tggagacagg aagtcatgtg gaacttctcc tccttaagaa cactagtgga
    52861 gacgagtggg aagttctggc taaacctgcc aaacgcctca aggtcggtac tcgtatcagc
    52921 tttggtgatg gccgcctcag cgctgtcgtt acagaagaat tgacccacgg gggacgcatt
    52981 gtccgctttg aataccaagg aattttccta gaagtcttgg aaagtctggg agaaatgcct
    53041 ctgccacctt atatccacga aaaattagat gaccgtgaac gttatcaaac cgtctacgcc
    53101 aaggaaagcg gctctgctgc agcaccgact gctggtcttc acttcaccaa agaactgctg
    53161 gcagaaatcc aagctaaggg tgttcatcta gtctatctga ctctccatgt cggactcgga
    53221 acctttagac ctgtttctgt ggataatctg gacgaacacg aaatgcattc agagttctat
    53281 caactttctg aggaagctgc tgccaccctt cgctctgtca aagaaaatgg tggtcgtgtc
    53341 atcgctgtcg gaaccacttc tatccgcacc ttggaaacta ttggttccaa gtttgatggg
    53401 caaatccaag cagattctgg ttggaccaat atctttatca aacctgggta tgagtggaag
    53461 gtcgtggatg ccttctcaac caacttccac ctgccaaaat caactctggt catgttggtt
    53521 tctgcctttg caggccgtga attagtctta gatgcctacc accatgccat ccaagaacac
    53581 taccgcttct tcagttttgg tgacgccatg tttatttatt gagaaagaat ttgtctaaat
    53641 cttcgaaatt aaaatgcttc cccctagctt cgaaaatatt gccatagatt gcgttgactc
    53701 tccaaattga ttcatctata ttttatttca gcttcctata ttttcttcgc tgtttgtaaa
    53761 tcaaaatgca agacacatga gtagcaccat atttgttact cttatctgtc ctctcaagag
    53821 actattatga gttatttcag aatcattcac tactttgacc ctgactctcc ttagtctcaa
    53881 aatcaaagac ttatactctt caaaaatctc ttcaaaccgc gtcaacgtca ccttggatta
    53941 tatatgtgac tgacttcgtc agttctatct acaacctcaa agcagtactt tgagcaacct
    54001 gcgactagtt ttctagtttg ctctttgatt ttcattgagt attaaacaaa aagtgaacaa
    54061 atctgaattc taatgtacag aagactaggc ttgttcactt ttttatagtc gctataagat
    54121 gaccttatct atagcttttt atatataatt atatattcag acatactatt atcaattttg
    54181 tcgcagggag gaatctgtta acgcacccat tcaccattat cattgactct atagccatct
    54241 atacttgtat tgaccgctaa ctcacccgat gcatttacat aataccattt accaccaact
    54301 tggaaccatt gattgacttt catagaaccg ttgctgttga ggtagtacca tgaaccatta
    54361 acttgtaccc aacctgttgc catggaacca tcagtattat aaaaatacca cataccattt
    54421 tcttgtttcc agtctgttgt tggagcaact gctttagctg gttctactgc tacatctgtt
    54481 ccttggttag atgtaacaga tacaggatac gaaggaatag atgattgctc aggaacaaca
    54541 actttttcag gttctctcgt ccctctcctt atacgtcttt ttaccatctc tttagtaatt
    54601 tgacgagaag tagtttcttc aattgttcca tcacgttcat ctacagtata gattgtagta
    54661 agagtaattt accaatttct cctacttctt ctacttcttg acttttatca agagttgggt
    54721 catcgagata ttctgtttcg attggaattt cttggacaag aacttggggc ttggttcctt
    54781 ttttaacaac tcttgtttga gagtcttctt tttgacttaa agtactctca gttacttgtc
    54841 cactctttcc atctacatca taagttatcg ttgtaactgt tttcccattc tttcctagag
    54901 taatctcttg ctcctgtcct gcagaaaggt cattgtctgc ttcatattta gtagcacatg
    54961 gaacaagaac ttcttcaacc ttgcttttag ctggaacttt gataactgta tccgtggctt
    55021 gttttctatc aacagtaacc tgttcggtaa catgaccagt ctctggatta acatcgtagg
    55081 tccttgtcgt agttacatag ccatcctctc catcaattgt aacaggattt tcactacggt
    55141 cttttgtttc atctttttca taacgaattc gcgtacttga aattttcttg gttactacct
    55201 taggtttagt cgctactttt acaataatac ccccattgtc agcgtcatca tactctattc
    55261 cctcttcttt atctctagta tcatctctga catattgaat cccatcagca gcatgaacaa
    55321 aacttgtatt cagattcctc ctaaaaataa agttagcccg attaccgcag aaccaaaaat
    55381 ctttccgagt ttacgtaatg catagcgctt attagtatta gattttgcca ttacatccta
    55441 cttctagtat agcatctttt ctatcaaacg ttaaacaata tactttatat ataaaataga
    55501 cttagaatga tatattgatt attgaactaa cactttaact atatcgtaat caatctcata
    55561 tataaaggat tgcagacatc ttatctaaat acatgctaat atatttagat acaaacattc
    55621 caacttgata attttcactc atctttcatc attccttata caactatgca gtataaatag
    55681 aatagttttc tcatcagaat gagactattt taatattaga taatttgata gaaaaaacat
    55741 gaataaaatt ggttgaattt aattccctaa ctcgatagag gattctattt taaatttaac
    55801 aacagctagg attacactat aaaaagtaca ttaccacctg ttcggctgga gaatctagtc
    55861 gattagctcc ctcttagtta caagcttaaa gcgatttttt aactaatact aaaccagatt
    55921 aaagaaggaa ctagaacatc tgtcttgtac taatcaatat gctattttaa agtttcaaat
    55981 aaacttggaa tgctaattat tatatttaca ataaatagtt gacttcgata gaaacagaaa
    56041 atagaattca acattcttcc ctacttatac cataataaat aagatccgca agatagcctt
    56101 ttctctctac accattaaca atggtttgta gataggacat tccagccttt tccatgacac
    56161 gacctgaagc tgggttgaga ctggcataac gtgctctgac cttttgaaaa cctgcttgag
    56221 taaaacaaaa gtccaagata gctttcaaag tctctgtcat cataccattt ccccagtaag
    56281 ccttgcctaa cacatagcca atttcacagc ttaaatcagc ctcgtctatc ttaacgatgc
    56341 tgatatctcc tatcacttgc tctgggtttt cttttagaca aatggcccat ttataatagt
    56401 tgagattagt ataggaagca acccaattgc gaatcgagtt tcgagtgatt tcgatatcag
    56461 gatggggatc ccaggtaaca taggtcagat tctcagcaga tgaagcccaa ttttgaaaca
    56521 tggcttctgc atcactctcc acaaatcttc ttaaaatcaa acgttctgtc tgtaatattt
    56581 gcgtaccgat agatttcatg atgctacctc acttccttat ttctatgctc gtagtaaaaa
    56641 ggctcaactc agtaacagct agaaaacgag tttgtcttct aatttcttga cgcagtcgct
    56701 gcctggtttg aaatgcgctc tagcaagctt tttcaaacct agtcatcgtt gcaggggtaa
    56761 gacgatgaaa tcgaactctt cgagttaccg aatctgcctc attttcttat ttctaaactc
    56821 ggggttcaag atcccccgga cctttttact cccaaaagtc atcaaataca gtgatgggta
    56881 agtggcgttt gtgttggcct ttgtgccacc agttttcaat ggtcgcttga gcttctgggc
    56941 tgattgtttt gccttctagg tagtcgtcaa tctctgcgta ggtgactcca agtgcgactt
    57001 cgtcagctag gcctggttta tcttcttcta ggtctgccgt tgggattttt tcataaaggg
    57061 ctggctctgc gccaagtttc tgcaagagct gttttccttg gcgtttattg aggcggtaaa
    57121 gagggagaat atccgcaccg ccgtcaccaa acttggtaaa gaaacctgtg atattttccg
    57181 cggcgtggtc tgttccaatg accgctccgc tatgggaacc agcaagggca tactgagcaa
    57241 tcatacggca acgtgccttg atattcccct tgttgaagtc tgaaacagga ctacctgtcg
    57301 cttcaactgc agctgtcatg gcatcagctg attccttgat attcacaacc aagctgacat
    57361 ctggctggat gaaggctagg gctttttgag catctgcttc atcagcttgc actccgtatg
    57421 gcaggcggac agcgataaat ttgtagctgt cgtctcccgt ttcagctcgc agttcttcca
    57481 tagctaattg cgccaaacgc cctgccaagg ttgagtcttg tcccccagaa atccctagta
    57541 caaaggtttt taggaaggga tgttttttca gatatctttt taagaaatca atagaacgac
    57601 ggatttcttc ctgggcatca atcactggtt tgacacccag ctcttggata atcgtttctt
    57661 gcaaactcat tcttcttctc cttcaccaag ggcttccttg cgcatcttat caatcaagtc
    57721 catcttatct tgccatacat cacgcgccaa atccactgga tagtgctgcg gattgagcac
    57781 acgcttatac tcatcccaca acttgtcaaa ttccttacgg gcataatcct gaatgtcagt
    57841 caaactaggc aagttgtaaa ctaatattcc ttctttgaag atatccacca agagaggaac
    57901 ggcatcaaaa ttacgaaccg tcttcttgat gtatgtatag gtcggatgga acatcttaat
    57961 ttctgtcatg tcgctaatat ccacaccatc ataagtgatg tagtcacctt ctgacttgcc
    58021 tttttcacga ctggtaatgc gccacacctg cttcttacct ggcgtcgaca ctttttccgc
    58081 attattagac agcttaatcg tattgcgcat ctggccgttt tcatcttcga ttgcaacaat
    58141 cttgtaaacc gccccaagag ccggctggtc ataggctgta atcagcttgg tacccacacc
    58201 ccagacatca atcttggcct tttgcatctt gaggttaagg atggtatttt catctagatc
    58261 attagaagca taaatcttag cctctggaaa tccagcctcg tccagttgct gacggacttt
    58321 cttagaaatg taggcaatat ccccagagtc aatccgcaca cccataaagt taatctgatc
    58381 acccagctca cgcgccacct gaatggcagc tggtacaccg atgcgaaggg tatcataggt
    58441 atccacaaga aagacacaat ttttgtgggt cgcagcgtaa gccttgaaag cctcatagtc
    58501 attgccataa acctgtacca aggcatgggc atgggttccc aaaacaggaa tgtcaaagag
    58561 cttacccgca cgcacgttgc tggttccatt ggcgccacca atcaccgctg cgcgtgttcc
    58621 ccagatggcc gcatccattt cttgagcccg acgtgtccca aactccatca agggttcatc
    58681 ttcgataacc gaacgaatac gagctgcctt cgtcgccacc aaggtctggt agttgacgat
    58741 gttcaaaaga gccgtttcga ccaactgaca ttgggctaga ggtccttcca cctgcacaat
    58801 cggttcatta gcaaaaacca aatccccttc ttgggcagaa cgaacggtca actccaactt
    58861 gaaattgcga aggtaatcca agaacgcccc atgataacca agcgactcca aataggctat
    58921 atcactatct gaaaaacgca agtcttcaag atagttcaca attctttcca aacctgcaaa
    58981 aaccgcatag ccgttcttaa aaggctgttg gcggaaatac acctcaaaga ccgccttctt
    59041 attgtaaatc ccttgatcaa agtaaacctg catcatgttg atctggtaca agtccgtgtg
    59101 caatgtcaaa ctatcatctg gatacatact tttcctactt ccttagctag aaacccatga
    59161 aaattttcaa gaactttcat gtattccaat aaattagtac tattatatca cattttagct
    59221 ggattgagaa aagagtaaca agctattctc cactctccaa ttcatccata tcttgttcaa
    59281 attttttctg agcccattcg ccatagctct taagaccaag attgccaata aagacccacg
    59341 gaaggtaaat gacataagta atgacccaag cagacaggta tttaaaattc aaaggattgt
    59401 gctgataaat ttctatgttg aattgataat tctgcaacat caaaagagcc gtaatagcca
    59461 aggttaggaa aaaacaaccc aaaatcgtaa aatgaaaacg actatagtag gtcactccca
    59521 gataacgggc acggttaaaa aagtaaaatg tccctatgat gataacgatt agcagcatat
    59581 tagaattaaa aaggcttggt gctaatactg aaatgatata agataggagc gacaaagcga
    59641 tgcagatata gaaactttca gagcccgctt tattgatcag ttgttcttct ctttcgtcta
    59701 gtaattgata ataataaaat ctatttttca tcttcttcct cccaaaatag ttggtctagg
    59761 gttttcccta aacatctgca aatagactgg cagagcgaga gactgggatt gtattttccc
    59821 gcctctatca aaccaatagt ctggcgtgtc accccgacag cctctgccag ttgaccttgt
    59881 gttaaatcac gctctacccg agctaatttt aattttaaat ttttagccac cttcgtcctc
    59941 cttatagttt taatactcat ctactcttaa aaaatccaaa accaacacaa gctatcagct
    60001 atttattata accccattaa tttgcactcc agtattatct tcagccgaaa atcagtattt
    60061 taggttgttt gaaataaact tcctagttta ctcttttatt tttatttagt attagcttta
    60121 gtaggatctt ttaaacgagt ttttattgct tatatttctt gtttagttta gttggtttca
    60181 tatcactatt atataatgct tttggaaatg agcctagtat aagtcaaaaa cgtgttgaat
    60241 agtgaaagca ggagcatacc aagatgactg atttttttat tgtattttag gtatgcaaaa
    60301 taatactaaa tgaaactaaa atgaatactt cttgcttttc ttgagagtga aaaagtacaa
    60361 taaaatgatt gtagtgatta ccacaagata ctgactaata gacatcgtat ttgaagccac
    60421 aaatacaggt aaagaataaa aaggaagaag ttcaattgct ttgataaaca aatcattact
    60481 agtattcatc atcatcgtat ttaatatatt agggataaag agtagtaaag ctactaaaat
    60541 accaaatacc aagccacttt tcaatttgta taaatagaaa atgtaagata atagaaaata
    60601 aaacaggact aacaaaattg tattgactac aatagctact ataaaatcta atcctaaatc
    60661 acctggaata cccaatttac ttactatgac tagtaacaca atgccattca aagcataaat
    60721 aatggaaact gagatagcat atagaaaatt agacaagacg taaatcatcc gattcttctg
    60781 tttatttaat ttcatgatta taaccttcct ttattttgaa cgatttaggt aaatatcttt
    60841 aagcgtctct tttttagttt caaaatcaac tactttaata gagttttcgt taaaaaattt
    60901 aaaaagctca ctattttgaa tattcccaga catagtaatt ctcaatccct cttcctaaac
    60961 aatatcccaa aattcttgtt tggtaatgaa aatgtctcta tctgtagctg atgaaaaagc
    61021 tatctcaaac aagaaattat gactatcttt tcctactttt tgaaatgtca aaagcccgtt
    61081 ctccaagaaa agaactctct cacaaatttc ttcaatgtct tctaatttat gactcgatat
    61141 taaaattccc acattttcat gtaaagctaa tttttttaga accgctaaaa ctatttgtga
    61201 tgattcaata tctaaaccat tagtcggttc atctaaaatc aatatatcag gttccgtaac
    61261 gagagttaga agcaaagcta atttttgttt tgtacccaaa gaataggtct ttactttttt
    61321 attaatagac tgagtcaaat ctaactcttg gatcaaacat ctaaatcttt cttgattgta
    61381 gtcaacacca tataaatttg acaaatattt taaattctct aaacctgttt tagataaaaa
    61441 taattttggt tcttcgatta aatacccaac attatcactg cttataatat tacctgaagt
    61501 cggttgatta ttctgaacaa gaattttcat caacgtactc ttaccaactc catttctccc
    61561 tgctagacca acaatttcac ccttgttaag tgcaaaattt atatctttga gaatgattga
    61621 atgaccataa tgcttactta cattttttat tcctccatta atattattgg ttttctcttt
    61681 gattctcatt aattttaaaa agttgcgcct ctttttaaca atattatatc atatatctaa
    61741 cttaatgcaa tatataaatg tcattttgtg aaattttatt aacaaaaaac tctctacaac
    61801 aaaataatag agagttttct gcttattaat ttttttcaaa atatctaaga catcgcttct
    61861 ttgtttaatt tgataggctt tgatgcctaa actccgactt tatcacttaa aaaataaaaa
    61921 agacagctaa tctcttgcgc atcaagggat ttcgccattc tttaatctcg tttaagtgtt
    61981 gtatatggag ctttgcatag ttttttaaaa ctaaacaact acattaagca cgaactgtga
    62041 cgctggttcc atcttcctta taaagattga taagcccctc tttaagagct cgaaccatgt
    62101 ctcctgattg aacaggttgt gggactttaa tagtcaatag ttccattgga ttgggagcgc
    62161 ggtcgatttt attgccttta gcatcatgca aatcttcaat ataggtttca aaatgacgga
    62221 aacctggacc ataaaactca acttggtccc cttcgttaat gacgtttcgt tgacgaat
//
LOCUS       DAVBDG010000004        59959 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBDG010000004 DAVBDG010000000
VERSION     DAVBDG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.004"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..129
                     /locus_tag="VQ526_000213"
     CDS             <1..129
                     /locus_tag="VQ526_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2553990.1"
                     /translation="LKINEGLNIGSVRSPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            147..1031
                     /locus_tag="VQ526_000214"
     CDS             147..1031
                     /locus_tag="VQ526_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2553991.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(1047..1898)
                     /locus_tag="VQ526_000215"
     CDS             complement(1047..1898)
                     /locus_tag="VQ526_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2553992.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            2098..4155
                     /gene="pbp2b"
                     /locus_tag="VQ526_000216"
     CDS             2098..4155
                     /gene="pbp2b"
                     /locus_tag="VQ526_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2553993.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGN
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            4166..4762
                     /gene="recR"
                     /locus_tag="VQ526_000217"
     CDS             4166..4762
                     /gene="recR"
                     /locus_tag="VQ526_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2553994.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            4957..6000
                     /locus_tag="VQ526_000218"
     CDS             4957..6000
                     /locus_tag="VQ526_000218"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2553995.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFAKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            6084..7457
                     /locus_tag="VQ526_000219"
     CDS             6084..7457
                     /locus_tag="VQ526_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2553996.1"
                     /translation="MKLTIYEVAQAVGAKNDVSLFADAQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLTP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQIILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            7444..8055
                     /locus_tag="VQ526_000220"
     CDS             7444..8055
                     /locus_tag="VQ526_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2553997.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDFLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            8146..8850
                     /locus_tag="VQ526_000221"
     CDS             8146..8850
                     /locus_tag="VQ526_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2553998.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            9069..10442
                     /gene="ftsA"
                     /locus_tag="VQ526_000222"
     CDS             9069..10442
                     /gene="ftsA"
                     /locus_tag="VQ526_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2553999.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            10459..11718
                     /gene="ftsZ"
                     /locus_tag="VQ526_000223"
     CDS             10459..11718
                     /gene="ftsZ"
                     /locus_tag="VQ526_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2554000.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            11723..12394
                     /locus_tag="VQ526_000224"
     CDS             11723..12394
                     /locus_tag="VQ526_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2554001.1"
                     /translation="MNVKENTELVFREVGEASLSAHRESSSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            12404..12943
                     /locus_tag="VQ526_000225"
     CDS             12404..12943
                     /locus_tag="VQ526_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2554002.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            12943..13206
                     /locus_tag="VQ526_000226"
     CDS             12943..13206
                     /locus_tag="VQ526_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2554003.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            13203..13988
                     /locus_tag="VQ526_000227"
     CDS             13203..13988
                     /locus_tag="VQ526_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET2554004.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELNLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLFQDKGQTKKEKKKITVQLLLSK"
     gene            13997..14785
                     /gene="divIVA"
                     /locus_tag="VQ526_000228"
     CDS             13997..14785
                     /gene="divIVA"
                     /locus_tag="VQ526_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2554005.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            15133..17925
                     /locus_tag="VQ526_000229"
     CDS             15133..17925
                     /locus_tag="VQ526_000229"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2554006.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKNLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAAVSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            18328..18708
                     /locus_tag="VQ526_000230"
     CDS             18328..18708
                     /locus_tag="VQ526_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554007.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGW
                     VGGILVIIGGSLFLATLKKFKSEE"
     gene            18902..19192
                     /locus_tag="VQ526_000231"
     CDS             18902..19192
                     /locus_tag="VQ526_000231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554008.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            19243..19935
                     /locus_tag="VQ526_000232"
     CDS             19243..19935
                     /locus_tag="VQ526_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2554009.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            19988..22399
                     /locus_tag="VQ526_000233"
     CDS             19988..22399
                     /locus_tag="VQ526_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2554010.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            22649..23350
                     /locus_tag="VQ526_000234"
     CDS             22649..23350
                     /locus_tag="VQ526_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554011.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            23352..26060
                     /locus_tag="VQ526_000235"
     CDS             23352..26060
                     /locus_tag="VQ526_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2554012.1"
                     /translation="MIKRKTYWKDLIQSFTDSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDREIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGRAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(26156..26647)
                     /gene="tpx"
                     /locus_tag="VQ526_000236"
     CDS             complement(26156..26647)
                     /gene="tpx"
                     /locus_tag="VQ526_000236"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2554013.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(26770..27699)
                     /gene="psaA"
                     /locus_tag="VQ526_000237"
     CDS             complement(26770..27699)
                     /gene="psaA"
                     /locus_tag="VQ526_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2554014.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(27731..28579)
                     /locus_tag="VQ526_000238"
     CDS             complement(27731..28579)
                     /locus_tag="VQ526_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2554015.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(28576..29298)
                     /locus_tag="VQ526_000239"
     CDS             complement(28576..29298)
                     /locus_tag="VQ526_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2554016.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            29589..31481
                     /gene="pepO"
                     /locus_tag="VQ526_000240"
     CDS             29589..31481
                     /gene="pepO"
                     /locus_tag="VQ526_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2554017.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(31604..32233)
                     /locus_tag="VQ526_000241"
     CDS             complement(31604..32233)
                     /locus_tag="VQ526_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2554018.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            33229..35451
                     /locus_tag="VQ526_000242"
     CDS             33229..35451
                     /locus_tag="VQ526_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2554019.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            35481..35924
                     /locus_tag="VQ526_000243"
     CDS             35481..35924
                     /locus_tag="VQ526_000243"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2554020.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGFLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(36191..36376)
                     /locus_tag="VQ526_000244"
     CDS             complement(36191..36376)
                     /locus_tag="VQ526_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554021.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRELTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(36395..36808)
                     /locus_tag="VQ526_000245"
     CDS             complement(36395..36808)
                     /locus_tag="VQ526_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554022.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLD
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGILTIDFLFFVLLAFISSQRFSSLMP
                     IITARHSTFYYMTADYLGENYPDFYDKHISLWECL"
     gene            complement(36939..37586)
                     /locus_tag="VQ526_000246"
     CDS             complement(36939..37586)
                     /locus_tag="VQ526_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HET2554023.1"
                     /translation="MVVGIAIASGYLNSRLHKFVDWGLWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQE
                     GRPNQMSQKQVKLAPIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            complement(37612..37803)
                     /locus_tag="VQ526_000247"
     CDS             complement(37612..37803)
                     /locus_tag="VQ526_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554024.1"
                     /translation="MSRKSTNFSSPLPTLKVLFSGFYFYHFILLSFGVRIFEKNGRIF
                     EKKKGSLTYEKITKNFCYI"
     gene            complement(37784..38434)
                     /locus_tag="VQ526_000248"
     CDS             complement(37784..38434)
                     /locus_tag="VQ526_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2554025.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            38546..39388
                     /locus_tag="VQ526_000249"
     CDS             38546..39388
                     /locus_tag="VQ526_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2554026.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEDFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            39464..39901
                     /locus_tag="VQ526_000250"
     CDS             39464..39901
                     /locus_tag="VQ526_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2554027.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            39969..40271
                     /locus_tag="VQ526_000251"
     CDS             39969..40271
                     /locus_tag="VQ526_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554028.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(40362..41435)
                     /locus_tag="VQ526_000252"
     CDS             complement(40362..41435)
                     /locus_tag="VQ526_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2554029.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            41591..42268
                     /locus_tag="VQ526_000253"
     CDS             41591..42268
                     /locus_tag="VQ526_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2554030.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            42261..43235
                     /locus_tag="VQ526_000254"
     CDS             42261..43235
                     /locus_tag="VQ526_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2554031.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALVFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(43284..45227)
                     /gene="thrS"
                     /locus_tag="VQ526_000255"
     CDS             complement(43284..45227)
                     /gene="thrS"
                     /locus_tag="VQ526_000255"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2554032.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFIISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVNYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(45285..45608)
                     /locus_tag="VQ526_000256"
     CDS             complement(45285..45608)
                     /locus_tag="VQ526_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2554033.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            46467..47096
                     /locus_tag="VQ526_000257"
     CDS             46467..47096
                     /locus_tag="VQ526_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2554034.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     FYIDETKTTRLLDTSDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            47237..47506
                     /gene="rpsO"
                     /locus_tag="VQ526_000258"
     CDS             47237..47506
                     /gene="rpsO"
                     /locus_tag="VQ526_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2554035.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            48322..48936
                     /locus_tag="VQ526_000259"
     CDS             48322..48936
                     /locus_tag="VQ526_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2554036.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAILGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(49229..49978)
                     /locus_tag="VQ526_000260"
     CDS             complement(49229..49978)
                     /locus_tag="VQ526_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2554037.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            50111..52447
                     /locus_tag="VQ526_000261"
     CDS             50111..52447
                     /locus_tag="VQ526_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET2554038.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            52976..54439
                     /locus_tag="VQ526_000262"
     CDS             52976..54439
                     /locus_tag="VQ526_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2554039.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKV
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIEMTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFTELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            54432..54860
                     /locus_tag="VQ526_000263"
     CDS             54432..54860
                     /locus_tag="VQ526_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2554040.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            54838..55287
                     /locus_tag="VQ526_000264"
     CDS             54838..55287
                     /locus_tag="VQ526_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2554041.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            55315..55626
                     /locus_tag="VQ526_000265"
     CDS             55315..55626
                     /locus_tag="VQ526_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET2554042.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            55652..56737
                     /locus_tag="VQ526_000266"
     CDS             55652..56737
                     /locus_tag="VQ526_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HET2554043.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            56748..57443
                     /locus_tag="VQ526_000267"
     CDS             56748..57443
                     /locus_tag="VQ526_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2554044.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLKSTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            57480..59450
                     /gene="tkt"
                     /locus_tag="VQ526_000268"
     CDS             57480..59450
                     /gene="tkt"
                     /locus_tag="VQ526_000268"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2554045.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYKDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            59823..>59959
                     /locus_tag="VQ526_000269"
     CDS             59823..>59959
                     /locus_tag="VQ526_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2554046.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YG"
BASE COUNT        17665 a        10987 c        13617 g        17690 t
ORIGIN      
        1 ttgaaaatca atgaaggctt gaatattgga tctgttcgtt caccattgac accagtaact
       61 gaagaagatc gtccagttgt agaagcggct gcagccttga ttcgtgaaac caaggagcgc
      121 ttcctctaat ctaaaaggag gtatttatga catactacgt tgcaattgat atcggtggaa
      181 ctaacatcaa gtatggtttg gttgatcaag aggggcaact tcttgaatcg catgaaatgc
      241 caactgaggc gcataagggt ggacctcata tcttacaaaa gaccaaagat atcgtagcta
      301 gttatttaga aaaaggccca gtagcaggtg ttgccatatc ttctgctggg atggtggatc
      361 cggataaggg tgagattttc tatgctgggc cgcaaatccc taactacgca ggcacccagt
      421 tcaaaaagga aatcgaagaa agctttacta ttccttgtga gattgaaaat gatgtcaact
      481 gtgcaggtct tgctgaggca gtatctggtt caggcaaggg agcaagtgtg acactttgct
      541 tgaccattgg aacaggtatc ggtggttgct tgattatgga taggaaagtc ttccatggtt
      601 ttagtaattc agcctgtgaa gtcgggtata tgcatatgca ggatggagct tttcaagact
      661 tggcttctac aacagcttta gtgaaatatg tagctgaagc ccatggagaa gatgttgatc
      721 agtggaatgg ccgacgtatc tttaaggaag ctactgaagg aaacaaaatc tgcatggaag
      781 gtattgaccg tatggttgac tatctaggaa aaggtctggc aaatatttgc tacgttgcca
      841 atccagaagt ggttattctt ggtggtggta tcatggggca agaggctatc ctcaaaccta
      901 agatccgcac agccttgaaa gaggctttgg taccaagttt agcagaaaaa acacgattag
      961 aatttgccca tcaccaaaat acagcaggga tgttgggtgc atattatcat tttaagacaa
     1021 aacaatccta gtttggctca gccaaactag gattttctta cacgtttttg tctacgatag
     1081 ccgttgagtt ttttattttc ccagtagcta ttaaagattt tttccttgct ttcgcgattg
     1141 atttccaaaa agtaggcata aatcaaatcg ataaagaaga gcataggaag ttgagcggat
     1201 attcgttgga tataggaggg ttggctgtgg gtggctacaa gaacagtctc tgtataggtc
     1261 tggctatctt tattgggaac acttgtaaag agtacagtct ttgcccccat ctccttggca
     1321 tctaatagac tatctaaaat agaaggagtt gagcctgaaa gtgagaagcc cagtactaga
     1381 caattttcat ccatgatgct ggttgtccag gcaaagccgt cttggtctgt caaagcttcg
     1441 cagaccacac ctagtcgcat aaaacgtaat ttcatttcac gggcgacgag gccagaactc
     1501 cctgttccga agaagtagat acgctcagca tcttcgatta gctgggcaat tcgttctagt
     1561 tggatttcgt caatcaagtc ttgtgtttgt tccctcatgt tgctataact tctgaggact
     1621 cgtttggtca gtggactgtg cttggagact tggttggctt gattttctgc ctgatgttgg
     1681 tattggaaaa taaattctcg gtagccagta aagccacact ttttagcaaa gcgggtcaaa
     1741 gcagcttgag aaatatgtaa tttttgggtg acttgttgag aagataaatc atctgtaatc
     1801 gtttcagctt gcaaaaaata gcgagcgatt tcttgttcta ggtctgtcat ttcttcaaaa
     1861 tgtgaatcaa tgatagttgc gatatctggt ttgtccatag ggaaagctcc tttacatgag
     1921 tcatactgga agactagatc agggaatagt cacacttcat tataacacat aatataagga
     1981 tagataaata aaaacgcatc tctgttttaa aaacgaaaaa atcgaaaaag cttctctctt
     2041 ttccataatt ttctactcaa attgtggtac aattaagagt aagattttaa gttagaaatg
     2101 agactgattt gtatgagaaa atttaacagc cattcgattc cgattcggct taatttattg
     2161 ttttcaatcg tcattttact ctttatgacc attattggtc gtttgttgta tatgcaggtt
     2221 ttgaacaagg atttttacga aaaaaagcta gcctcagcta gtcagaccaa gattacaagc
     2281 agttcagccc gtggggaaat ttatgatgct agtggaaaac ctttggtaga aaatacgtta
     2341 aagcaggttg tttcctttac gcgtagcaat aaaatgacag ctacagactt aaaagaaaca
     2401 gctaaaaagt tactgactta tgtgagcatc agttctccaa atttgacaga acgccagctg
     2461 gcggattact atttggctga tcctgaaatc tataaaaaaa cagtggaagc tctcccaagc
     2521 gagaaacgct tggattcaga tggcaatcgt ctatccgaat cagaactgta taacaatgcg
     2581 gtcgatagtg tacaaacgag tcaactaaac tatacagagg atgaaaagaa agaaatctat
     2641 ctttttagtc agttaaatgc tgttggaaac tttgcgacag gaaccattgc gacagatcct
     2701 ctaaatgatt ctcaggtggc tgttattgcc tctatttcaa aggagatgcc tggcattagt
     2761 atttctactt cttgggatag aaaggttttg gaaacttccc tttcttctat agttgggagt
     2821 gtatccagtg aaaaagctgg tctcccagcg gaagaagcag aagcctatct taaaaaaggc
     2881 tattctctaa atgatcgtgt aggaacttcc tatttggaaa agcaatatga agagacctta
     2941 caaggaaaac gctcggtaaa agaaatccat ctggataaat atggcaatat ggaaagcgtg
     3001 gatacaattg aggaaggtag taagggaaac aatatcaaac tgaccattga tttggctttc
     3061 caagatagcg tggatgcttt actgaaaagt tatttcaatt ccgagctaga aaatggtgga
     3121 gccaagtatt ctgaaggtgt ctatgcagtc gcccttaacc caaaaacagg tgcggttttg
     3181 tctatgtcag ggattaaaca tgacttgaaa acgggagagt tgacgcctga ttccttggga
     3241 acggtaacca atgtctttgt tccaggttcg gttgtcaagg cggcgaccat cagctcaggt
     3301 tgggaaaatg gagtcttgtc aggaaaccag accttgacag accagtccat tgtcttccaa
     3361 ggttcagctc ccatcaattc ttggtatact caggcttacg gttcattccc tatcacagcg
     3421 gtccaagctc tggagtattc atcaaatacc tatatggtcc aaacagcctt aggtcttatg
     3481 gggcaaacct atcaacccaa tatgtttgtc ggcaccagca atctagagtc tgctatggag
     3541 aaactgcgtt caacctttgg cgaatatggc ttgggtactg cgacaggaat tgacctacca
     3601 gatgaatcta ctggatttgt tcccaaagag tatagctttg ctaattacat tactaatgcc
     3661 tttgggcagt ttgataacta tacgccgatg cagttggctc agtatgtagc aactattgca
     3721 aataatggtg ttcgtgtggc tcctcgtatt gttgaaggca tttatggtaa taatgataag
     3781 ggaggactgg gtaacttgat tcagcaactg caaccgacag agatgaataa ggtcaatata
     3841 tccgactccg atatgagcat cttgcaccaa ggtttttatc aggttgccca tggtactagt
     3901 ggattgacaa ctggacgtgc cttttcaaat ggtgccttgg tatccattag cggaaaaaca
     3961 ggtacagccg aaagctatgt ggcagatggt cagcaagcaa ccaataccaa tgcggtggcc
     4021 tatgccccat ctgataatcc ccaaatcgct gtcgcagtgg tctttcctca taataccaat
     4081 ctaacaaatg gtgtaggacc ttccattgcg cgtgacatta tcaatctgta tcaaaaatac
     4141 catccaatga attagaaagg aaattatgct ttatccaaca cctattgcca agttgattga
     4201 cagttattct aagttaccag gtatcgggat taagacggct acgcgtctgg ccttttatac
     4261 gattgggatg tctgctgatg atgtcaatga atttgcaaaa aatctccttt ctgctaagag
     4321 agaattgaca tattgttcta tttgtggacg tttgacagac gacgatcctt gttctatctg
     4381 tactgatccg actcgtgacc agacaacaat tttagttctt gaggatagta gagatgtggc
     4441 agccatggaa aatatccaag aataccatgg actctatcat gtccttcatg gcctcatttc
     4501 tcctatgaat ggtatcagtc cggacgatat caatctcaag agccttatga ctcgtcttat
     4561 ggatagtgag gtttcagaag tgattgtggc gactaatgct acagcggatg gtgaagcgac
     4621 ttccatgtat ctttcacgtt tgctcaagcc ggctggtatc aaggttacgc gtctagcacg
     4681 aggtctcgct gtgggagcgg acattgagta tgcggacgaa gtgacactct tacgagcgat
     4741 tgaaaatcgg acagagttgt aagtgtaggc aaatttacga actccattca tttataaaaa
     4801 atcaaagagg ctgaaaatcg ttcctatcgg cctctttttg tatagtgtga tgagtaggat
     4861 caggttcaag ttttaaaaaa ccaagcaaat atgatatact aaagagcgag tattctagta
     4921 gaattaggac aaataagtag aattaggaca aataatatga aacaaacgat tattctttta
     4981 tatggtggac ggagtgcgga acgcgaagtc tctgtccttt cagctgagag tgtcatgcgt
     5041 gcggtcaatt acgaccgttt cacagtcaag actttcttta tcagtcagtc aggtgacttt
     5101 atcaaaacac aggaatttag tcatgctccg gggcaagaag accgtctcat gaccaatgaa
     5161 accattgatt gggataagaa agttgcacca agtgctatct acgaagaagg tgcagtggtc
     5221 tttccagtcc ttcacgggcc aatgggagaa gatggctctg ttcaaggatt cttggaagtt
     5281 ttgaaaatgc cttacgttgg ttgcaacatt ttgtcatcaa gtcttgccat ggataaaatc
     5341 acgactaagc gtgttctgga atctgctggt attgcccaag ttccttatgt ggctatcgtt
     5401 gaaggcgatg atgtgactgc taaaatcgct gaagtggaag aaaaattggc ttatccagtc
     5461 ttcgctaagc cgtcaaacat ggggtctagt gtcggtattt ctaagtctga aaaccaagaa
     5521 gaactccgtc aagccttaaa acttgccttc cgatatgaca gccgtgtctt ggttgagcaa
     5581 ggagtgaatg cccgtgaaat tgaggttggc ctcttgggta actacgatgt caagagcacg
     5641 ctacctggag aagttgtcaa ggacgttgcc ttttatgact acgatgccaa gtatattgat
     5701 aacaagatta ctatggatat tcctgccaaa atcagtgatg atgtggtggc tgtcatgcgt
     5761 caaaatgcag aaacagcctt ccgtgccatt ggtggccttg gtctatctcg ttgcgatttc
     5821 ttctatacag ataagggaga gatttttctc aacgagctca ataccatgcc aggtttcacc
     5881 cagtggtcta tgtacccact actttgggac aatatgggga tcagctaccc agaactaatc
     5941 gagcgtttgg ttgaccttgc caaggaaagt tttgacaagc gcgaagcgca tttgatataa
     6001 aatgaaagag agggtagaag ccagaaccat cactgcaagg tgactagagt tctcggactt
     6061 cgcccctttt taaaggagta gaaatgaaat taacaatcta tgaagttgcc caagctgttg
     6121 gagctaaaaa tgatgttagt ctttttgcgg acgcccagtt agaaaaggct gagtttgaca
     6181 gtcgattgat tgggactgga gatttatttg tgccacttaa aggtgcgcgt gatggccatg
     6241 actttattga aacagccttt gaaaatggtg cagcagtaac cttgtctgag aaagaggtct
     6301 caaatcatcc ttacattcta gtagatgatg ttttgacagc ctttcaatcc ttagcatcct
     6361 actatcttga aaaaacgact gttgatgtct ttgctgttac aggttcaaat ggcaagacaa
     6421 cgactaagga tatgttggcg catttactgt caacaagata caagacctac aaaacacaag
     6481 gcaattacaa taatgagatt ggccttcctt acacagttct tcatatgcct gaaggaacag
     6541 aaaagttggt tttggagatg ggacaggatc acttgggaga tattcatctc ttgtctgaat
     6601 tggctcgtcc aaaaacagcc atcgtgacct tggttggaga agctcatttg gcctttttca
     6661 aagatcgttc tgagattgct aaaggaaaaa tgcaaattgc agacggaatg gcttcaggtt
     6721 ccttgctttt aacgccggct gaccctatcg tagaggacta tttgccaact gataaaaagg
     6781 tggtccgttt tgggcaaggg gcagagttgg aaatcacaga cttggttgag cgtaaggaca
     6841 gtctgacctt taaggctaat ttcttggaac aagcccttga tttgccagtg actggtaagt
     6901 acaatgctac caatgctatg attgcgtcct atgttgccct acaagaagga gtttcagagg
     6961 agcaaattca tcaggccttc caaggtcttg aattgacgcg taatcgtact gagtggaaga
     7021 aagcagccaa tggagcagat atcctgtcag acgtatataa tgccaatcca actgctatga
     7081 agctgatttt agagactttc tctgccattc cagccaatga aggtggcaag aaaattgcag
     7141 tgttggcgga tatgaaggag cttggtgacc agtctattca actccataac cagataattt
     7201 tgagcctttc tccagatgtg cttgataccg ttattttcta tggagaagac attgctgaat
     7261 tagcccaact tgctagtcaa atgttcccaa tcggacacgt ttattacttc aagaaaacag
     7321 aagaccaaga ccaatttgaa gacctagtta agcaggtcaa agaaagcctt ggggctcatg
     7381 accaaatcct gctcaaaggc tctaactcta tgaatctagc caagttggta gaaagtttag
     7441 aaaatgaaga caagtgattt tgtcaagtat ttgcaaagaa tgattgccat tacagatact
     7501 ggcttaacct ttacaaaaga tccgtttgac cgtgagcgct acgaagactt gcgagacttt
     7561 ttatctgaaa tgttgaatca agcatcagac cttgattccg aagaagtggc agaagtcttg
     7621 aagccaactt ctgcttatgc gactccgtta atggacgtcc gtgcttggat tgttgaggac
     7681 gaaaagattt gtctggttag gggacaagga gaggatagtt gggctttgcc aggtggcttt
     7741 ggtgaggttg gctattctcc aacagaaaat attctcaagg aaattgaaga agaaaccggt
     7801 tttaaagcca aagttgaaag actgctagct gtttttgata ccaatcgttt ccaactacag
     7861 agcaaacagt atacaaagtt tgtttttgga tgtaagcttc tcgatggaca attccaagaa
     7921 aatcaagaaa ttgctgacct tcaatttttt gccattgacc aactgccgaa cttatctgaa
     7981 aaacgcatta ccaaggagca aatagagctt ctttggcagg tttatcaagg tcataggggg
     8041 caatatcttg actaagaaga tgattatcgt atttctaaat ccatttttaa caactagcat
     8101 ggtataataa tatgcaggaa aattttgaat tatgaggaag actagatgaa tttatgggat
     8161 attttcttta cgactcaggc aaccgagccg cccaaatttg accttttttg gtatgttagc
     8221 ctatttacgc tcttagcctt aaccttttat acagcccatc gctatcgtga aaagaaggtt
     8281 taccaacgat ttttccaaat cttgcagact gttcagttaa tccttcttta tggttggtac
     8341 tgggttaatc atatgccact gtcagaaagc ctaccctttt accattgccg tatggctatg
     8401 tttgtggtac tcttgcttcc tggtcagtcc aaatataaac aatactttgc attattggga
     8461 acatttggga cattagcagc ctttgtttat ccagtgccag atgcttaccc ttttccacat
     8521 attaccattt tatcctttat ctttggtcat ttagcactct tggggaactc tctagtttat
     8581 ctattgagac agtataatgc gcgattgctg gatgtgaagg gaatttttct catgaccttt
     8641 gccctaaatg ccttgatttt tgtggtcaat ttggtgacag gtggcgatta cggatttttg
     8701 acaaaaccgc cattggttgg ggatcacggt ctagtagcta attatttact tgtttcaatt
     8761 gtgctggtag ctactatcag tttgactaag aaaatcttag aattcttttt agctcaagaa
     8821 gcagaaaaaa tgattgcaaa ggaagcttaa cacagagctt tcttttttgc tcttagagag
     8881 tttttacaag cagcttataa aataagaatt tctgaataga caaactcaaa aaatggctgg
     8941 gaaatttagg aaaaaagcaa gcacgattaa attttttgtg ttataatatt ttgtgaatag
     9001 ctatgcctat gtttagctat ggaataatac gaagtgcgaa acttggaaga tagagaggaa
     9061 gcgatgtaat ggctagagaa ggctttttta caggtctaga tattggaaca agctctgtca
     9121 aggtgcttgt ggccgagcag agaaatggtg aattaaatgt aattggcgtg agtaatgcca
     9181 aaagtaaagg tgtaaaggat ggaattattg ttgatattga tgcagcagca actgctatca
     9241 agtcagccat ttcccaagcg gaagaaaagg caggcatttc gattaaatca gtgaatgtcg
     9301 gcttgcctgg taatcttttg caggtagaac caactcaggg gatgattcca gtaacatctg
     9361 atactaagga aattacggat caagatgttg aaaatgttgt caaatcagct ttgacaaaga
     9421 gtatgacacc tgaccgtgaa gtcattacct ttattcctga agaatttatt gtggatggtt
     9481 tccaagggat tcgtgaccca cgtggcatga tgggggttcg ccttgaaatg cgtggtttgc
     9541 tttatacagg acctcgtact atcttgcaca atttgcgtaa gacggttgag cgtgcaggtg
     9601 ttcaggttga aaatgttatc atttcaccac tagcaatggt tcagtctgtt ttgaacgaag
     9661 gggaacgtga atttggtgct acagtgattg atatgggggc aggtcaaacg actgtcgcta
     9721 caatccgtaa tcaagaactc cagttcacac atattctcca agaaggtgga gattatgtaa
     9781 ctaaagatat ctccaaggtt ttgaaaacct ctcgcaaatt agcggaaggc ttgaaactga
     9841 attacgggga agcctatccg cctcttgcaa gcaaagaaac cttccaagta gaggttattg
     9901 gagaagtaga agcagtcgaa gtgacggaag cctacttgtc agaaattatt tctgcacgaa
     9961 tcaagcacat ccttgaacaa atcaagcaag aattagatag aaggcgtcta ttggacctcc
    10021 ctggtggtat tgtcttaatc ggtgggaatg ccattttacc aggtatggtt gagcttgctc
    10081 aggaagtctt tggcgtccgt gtcaagcttt atgtcccaaa tcaagttggt atccgtaatc
    10141 cagcctttgc gcatgtgatt agtttatcag aatttgcggg tcaattaaca gaagttaatc
    10201 ttttggctca gggagcgata aaaggtgaga atgacttaag tcatcagcca attagttttg
    10261 gtgggatgct gcaaaaaaca gctcagtttg tacaatcaac gcctgttcaa ccagctcctg
    10321 ctccagaagt agagccggtg gcgcctacag aaccaatggc ggatttccaa caagcttcac
    10381 aaaataaacc gaaattagca gatcgtttcc gtggcttgat cggaagcatg tttgacgaat
    10441 aaagaggaaa aataaattat gacattttca tttgatacag ctgctgctca aggggcagtg
    10501 attaaagtaa ttggtgtcgg tggaggtggt ggcaatgcca tcaaccgtat ggtcgacgaa
    10561 ggtgttacag gcgtagaatt tatcgcagca aacacagatg tacaagcatt gagtagtaca
    10621 aaagctgaga ctgttattca gttgggacct aaattgactc gtggtttggg tgcaggaggt
    10681 caacctgagg ttggtcgtaa agccgctgaa gaaagcgaag aaacactgac ggaagctatt
    10741 agtggtgccg atatggtctt catcactgct ggtatgggag gaggctctgg aactggagct
    10801 gctcctgtta ttgctcgtat cgccaaagat ttaggtgcgc ttacagttgg tgttgtaaca
    10861 cgtccctttg gttttgaagg aagtaagcgt ggacaatttg ctgtagaagg aatcaatcaa
    10921 cttcgtgagc atgtagacac tctattgatt atctcaaaca acaatttgct tgaaattgtt
    10981 gataagaaaa caccgctttt ggaggctctt agcgaagcgg ataacgttct tcgtcaaggt
    11041 gttcaaggga ttaccgattt gattaccaat ccaggattga ttaaccttga ctttgccgat
    11101 gtgaaaacgg taatggcaaa caaagggaat gctcttatgg gtattggtat cggtagtgga
    11161 gaagaacgtg tggtagaagc ggcacgtaag gcaatctatt caccacttct tgaaacaact
    11221 attgacggtg ctgaggatgt tatcgtcaac gttactggtg gtcttgactt aaccttgatt
    11281 gaggcagaag aggcttcaca aattgtgaac caggcagcag gtcaaggagt gaacatctgg
    11341 ctcggtactt caattgatga aagtatgcgt gatgaaattc gtgtaacagt tgttgcaacg
    11401 ggtgttcgtc aagaccgcgt agaaaaggtt gtggctccac aagctagatc tgctactaac
    11461 taccgtgaga cagtgaaacc agctcattca catggttttg atcgtcattt tgatatggca
    11521 gaaacagttg aattgccaaa acaaaatcca cgtcgtttgg aaccaactca ggcatctgct
    11581 tttggtgatt gggatcttcg ccgtgaatcg attgttcgta caacagattc agtcgtttct
    11641 ccagtcgagc gctttgaagc cccaatttca caagatgaag atgaattgga tacacctcca
    11701 tttttcaaaa atcgttaagt aaatgaatgt aaaagaaaat acagaacttg tttttcgaga
    11761 agttggagag gctagtctga gtgctcatcg agagagtagt tcggtctctg tcattgcagt
    11821 taccaagtat gtagatgtac cgacagcgga agccttgctt ccgctaggtg ttcatcatat
    11881 cggtgaaaat cgtgtagata agtttctgga aaaatatgaa gctttaaaag atcgagatgt
    11941 gacttggcat ttgattggta ccttgcaaag acgtaaggtg aaagatgtca ttcaatacgt
    12001 tgattatttc catgcattgg actcagtaaa gctagcaggg gaaattcaaa aaagaagtga
    12061 ccgagtcatc aagtgtttcc ttcaagtaaa tatttctaaa gaagaaagca aacacggttt
    12121 ttcgagagag gaactgctgg aaatcttgcc agagttagcc agactagata agattgaata
    12181 tgttggttta atgacgatgg caccttttga ggctagcagt gagcagttga aagagatttt
    12241 caaggcgacc caagatttac aaagagaaat tcaagagaaa caaattccaa atatgcctat
    12301 gaccgagtta agtatgggaa tgagtcgtga ttataaagaa gcgattcaat tcggttccac
    12361 ttttgttcgt ataggtacat cattttttaa gtaggagaga accatgtctt taaaagatag
    12421 attcgataga tttatagatt attttacgga ggatgaggat tcaagtctcc cttatgaaaa
    12481 aagagatgag cctgtgttta ctccagtaaa ttcttcacag gaaccggctc tcccaatgaa
    12541 tcaaccttca cagtcggctg gtacaaaaga gaacaatatc accagacttc atgcaagaca
    12601 acaggaattg gcaaatcaga gtcagcgtgc aacggataag gtcattatag atgttcgtta
    12661 tcctagaaaa tatgaggatg caacagaaat tgttgattta ttggcaggaa acgaaagtat
    12721 cttgattgat tttcagtata tgacagaggt gcaggctcgt cgttgtttgg actatttgga
    12781 tggagcttgt catgttttag ctggaaattt gaaaaaggta gcttctacca tgtatttgtt
    12841 gacaccagtg aatgttattg taaatgttga agatatccgt ttaccagatg aagatcaaca
    12901 gggtgagttc ggttttgata tgaagcgaaa tagagtacga taatgatttt tttaattcgt
    12961 atgatttata atgcagtgga tatttactcc ctgattttgg tagccttcgc tgtcatgtct
    13021 tggtttccag gtgcctacga atccagttta ggtcgttgga ttgtagcgtt ggtgaaacca
    13081 gtacttgctc ccttgcaacg cctgccttta cagatagcgg gtcttgattt atctgtttgg
    13141 gtcgcgattg ttttggttcg atttttagga gaaaacctag tgcgttttct ggcgatgata
    13201 ggatgaataa agggatttat cagcatttct ccatagaaga tcgtccattt cttgacaagg
    13261 gaatggaatg gataaagaag gtagaagata gctatgctcc ttttttaact ccttttatca
    13321 atcctcatca ggagaagcta ttaaagattt tggccaaaac ctatggtctt gcttgtagca
    13381 gtagtgggga attcgtctcg agtgagtatg ttcgagtttt attataccca gattatttcc
    13441 aaccagagtt ttcagatttt gaaatatctc tccaagaaat tgtgtattcc aataaatttg
    13501 aacatttaac gcatgctaag attttaggga cagtcatcaa tcaattaggg attgaacgga
    13561 aactttttgg agatatccta gtagatgaag aacgggcgca gattatgatt aatcagcagt
    13621 ttcttcttct ctttcaagat ggactaaaga aaattggtcg tatacctgtt tcgctggagg
    13681 aacgtccttt caccgagaaa atagataagc tagaacagta tcgagaactg aatttatctg
    13741 tgtctagttt tcgattagat gttcttttat caaatgtttt gaaactatct aggaatcaag
    13801 caaaccagtt gattgaaaag aaacttgtcc aagtaaatta tcatgtggta gacaaatcag
    13861 attacactgt tcaagttgga gacttgatta gtgtgagaaa atttggtcgc ttgagattat
    13921 ttcaagataa gggacaaacg aaaaaagaga agaaaaaaat aaccgtccag ttattattaa
    13981 gtaagtgagg aatagaatgc caattacatc attagaaata aaggacaaga cctttggaac
    14041 tcgattcaga ggttttgatc cagaagaagt cgatgaattt ttagatattg tggttcgtga
    14101 ttacgaagat cttgtgcgtg cgaatcatga taaaaatttg cgtattaaga gtttagaaga
    14161 gcgtttgtct tactttgatg aaataaaaga ttcattgagc cagtctgtat tgattgccca
    14221 ggatacagct gagagagtga aacaggcggc gcatgaacgt tcaaacaata tcattcatca
    14281 agcagagcaa gatgcgcaac gcttgttgga agaagctaaa tataaggcaa acgagattct
    14341 tcgtcaagca actgataatg ctaagaaagt cgctgttgaa acagaagaat tgaagaacaa
    14401 gagccgtgtc ttccaccaac gtctcaaatc tacaattgag agtcagttgg ctattgttga
    14461 atcttcagat tgggaagata ttctccgtcc aacagctact tatcttcaaa ctagtgatga
    14521 agcctttaaa gaagtggtta gcgaagtact tggagaaccg attccagctc caattgaaga
    14581 agaaccaatt gatatgacac gtcagttctc tcaagcagaa atggaagaat tacaagctcg
    14641 tattgaggta gccgataaag aattgtctga atttgaagct cagattaaac aggaagtgga
    14701 aactccaact cctgtagtga gtcctcaagt tgaagaagag cctctgctca tccagttggc
    14761 ccaatgtatg aagaaccaga agtagctcca gtgcatccga caggtccaac accagctaca
    14821 gaaactgttg attcagcatc aggatttgaa gcaccgcaag aatccgttac aattttataa
    14881 gaaacgatgt gagaacaata tcttatcctt atatttccag cgagcaggag atggtgtgag
    14941 tcctgcattc cctaacgata agattatcct ctcaaaaact caagtctgaa gctagtaaga
    15001 tttgacgttt cccacgttac gggataagag ggagaaagac taaatctttt tccgaataaa
    15061 ggtggtacca cgattttcgt cctttttgga agtcgtggtt tttaatttgt tattatttat
    15121 aaaggagata ccatgaaact caaagatacc cttaatcttg ggaaaacagc tttcccaatg
    15181 cgtgcaggcc ttccgaccaa agagccagtt tggcaaaagg aatgggaaga tgcaaaactt
    15241 taccaacgtc gtcaagaact aaatgaaggg aaaccgcatt ttgtcttgca tgatggccct
    15301 ccatacgcaa acggaaatat ccacgttgga catgccatga atcatatctc aaaagatatc
    15361 atcattcgtt ctaagtctat gtcaggtttt aacgcacctt atatcccagg ttgggatact
    15421 catggtctgc caatcgagca agttttggca aagcaaggtg tgaagcgcaa agaaatggac
    15481 ttggttgagt acttgaaact ctgtcgtgag tacgctcttt ctcaagttta taaacaacgt
    15541 gatgatttca aacgcttggg gatgtcaggt gactgggaaa atttatatgt gactttaact
    15601 ccagactatg aagcagccca aatccgtgtc tttggtgaga tggctaataa gggttatatc
    15661 taccgtggtg ccaagccagt ttactggtca tggtcatctg agtcagccct tgccgaagcg
    15721 gaaattgaat accatgattt ggtttcgact tcactttatt atgccaataa ggtaaaagat
    15781 ggcaaaggta tcctagatac agatacttat atcgtagtct ggacaacaac tccatttacc
    15841 atcacagctt ctcgtggttt gactgttggt gcagatattg attacgtttt ggttcaacct
    15901 gctagtgaaa ctcgtaagtt tgtcgttgct gcagaattgt tgactagctt gtctgagaaa
    15961 tttggctggg ctgatgttca agtgttggca acttaccgtg gtcaagaact caaccacatc
    16021 gtaacagaac acccatggga tacagctgta gatgagttgg taatccttgg tgatcacgtt
    16081 accactgact ctggtacagg tatcgtccat acagcccctg gttttggtga ggacgactac
    16141 aatgttggta ttgctaatgg tcttgaagtc gcagtgactg ttgacgaacg cggtatcatg
    16201 atggcgaatg ctggtcctga gtttgaaggt cagttctacg acaaggttgt accaactgtt
    16261 attgaaaaac ttggtaacct ccttctagcc caagaagaaa tctctcactc atacccattt
    16321 gactggcgta ctaagaaacc aatcatctgg cgtgcagttc cacaatggtt tgcctcagtt
    16381 tcaaaattcc gccaagaaat cttggatgct attgataaag ttaaattcca tacagaatgg
    16441 ggtaaagtac gtctatataa tatgattcgt gaccgtggtg actgggttat ctctcgtcaa
    16501 cgtgcttggg gtgttccact tccaatcttc tatgcagaag acggtacagc tatcatgaca
    16561 gctgagacta ttgaacatgt ggcgcaactc tttgaagtac atggttcaag catttggtgg
    16621 gaacgtgatg ccaaaaacct cttaccagaa ggatttacac atccaggttc tccaaatggg
    16681 gagttcaaga aagaaacaga tattatggat gtatggtttg actcaggttc atcatggaat
    16741 ggtgttttgg taaatcgtcc aaatctgact tatccagctg acctttacct tgaaggttct
    16801 gaccaatacc gtggttggtt caactcatca cttattacat ctgttgccaa ccatggcgta
    16861 gcaccttaca aacaaatctt gtcacaaggt tttacccttg atggtaaagg tgagaagatg
    16921 tctaaatcac ttggaaatac cattgctcca agtgatgttg aaaaacaatt tggtgcagaa
    16981 atcttgcgtc tctgggtaac aagtgttgac tcaagcaatg acgtgcgtat ctctatggat
    17041 attttgagcc aagtttctga aacttaccgt aagattcgta acactcttcg tttcttgatt
    17101 gccaatacat ctgactttaa cccagctcaa gatgtagtcg cttacgatga acttcgttca
    17161 gttgataagt acatgactat tcgctttaac cagcttgtca agaccattcg tgatgcctat
    17221 gcggactttg aattcttgac aatctacaag gccttggtga actttatcaa cgttgacttg
    17281 tcagccttct accttgattt tgccaaagat gttgtttaca ttgaaggtgc caaatcattg
    17341 gaacgccgtc aaatgcagac tgttttctat gacattcttg tcaaaatcac caaactcttg
    17401 acaccaatcc ttcctcacac tgcggaagaa atctggtcat atcttgagtt tgaagctgaa
    17461 gacttcgttc aattgtcaga attgccagaa gctcaaactt ttgctaatca agaagaagtc
    17521 ttggatacat gggcagcctt catggacttc cgtggacaag ctcaaaaagc cttggaagaa
    17581 gctcgtaatg caaaagttat cgggaaatcg cttgaagcac acttgacagt ttatccaaat
    17641 gaagttgtga aaactctact cgaagcagta aacagcaatg tggctcaact tttgatcgtg
    17701 tcagacttga ccatcgcaga agggccagct ccagaagctg ctgttagctt cgaagatgta
    17761 gccttcacag ttgaacgcgc tgcaggtcaa gtatgtgacc gttgccgtcg tatcgaccca
    17821 acaacagcag aacgcagcta ccaggcagtc atctgtgacc actgtgcaag catcgtagaa
    17881 gaaaactttg cggaagcagt cgcagaagga tttgaagaga aataaggttg aaaagtctag
    17941 gcaaaattca atttgagaag aaaagacaac taattttata gtctattaaa cgcattgtat
    18001 cacgtttttg aatacctgat atgatgcgtt ttttatttat tttaaaaatt tgcgaggtat
    18061 gactttttat actcaacaag aatcaaagag aaacttagca agctaacagt agtaagataa
    18121 aataggaatt tgatattagg gataagattg ctaaatagtg taatattttt acaataataa
    18181 atttatatag ttatttttgg tttctgaaaa gtattatatt ttatttcata ttatacaaat
    18241 ttttatttta taatatcaga aaatactttt tttaaaagca aatatgatac aattttattt
    18301 gaaaaaaata aaaaaggaga ttttattatg aaattaaaaa cacttgcttt aattagtggt
    18361 atcgtcggtc ttgtgggagg aattttactt cttattggtc cttttgtctt gttgggaaca
    18421 gcggtaaaca cagttgctac aactcttaat ggaggagcta ctgcaggggc tttttcaggt
    18481 gttgccttac tcttgaatgc cttgaagatt gcaaatcttg ttcttggtat cattgctatt
    18541 gtttactata aaggagataa tcgtgtaggt gcagctccgt ctgtactaat gattgttttt
    18601 ggtggagtta gtctcattcc attcttagga tgggttgggg ggattcttgt tattatcgga
    18661 ggatctctat tccttgcaac attgaagaaa ttcaaatcag aagaataaaa ggtattttag
    18721 catgaaaaga acaaaaaagt ttatcggtat aggagtagct ctattatctc tttttcttct
    18781 agttgcatgt ggaacataaa gttcaaagga tacttcaaca agtaatgatg agaagacagt
    18841 agcaacatcc aatagttcaa aagaaacaat cactttcgat acaccggttg taacagacga
    18901 tgtgattgaa tcaatacgca cttatgcaga ttatatagat ctttataaaa atatttttga
    18961 tgattatttt actaaagctg aggaaggttt caaaggcaca gctatggaaa ataatgactc
    19021 gtttactaaa ctaaaagagt caactcaaaa attattcgat gcgcagaaaa aaaggttaaa
    19081 taatgaagat agaatagaaa caactaaaaa caatgtgatt gccaaacatt gtcaaacagt
    19141 cctttccttt ttggttttga ctagcttttt tgtgaaaaat tgtgtaaaat agaatagata
    19201 aacgagggga aacctcggaa aatttaaagg agaatccatc taatggtaaa attggttttt
    19261 gctcgccacg gtgagtctga atggaacaaa gctaaccttt tcactggttg ggctgatgtt
    19321 gatttgtctg aaaaaggtac acaacaagcg attgacgctg gtaaattgat caaagaagct
    19381 ggtatcaaat ttgaccaagc ttacacttca gtattgaaac gtgctatcaa aacaactaac
    19441 ttggctcttg aagcttctga ccaattgtgg gttccagttg aaaaatcatg gcgcttgaac
    19501 gaacgtcact acggtggttt gactggtaaa aataaagctg aagctgctga acaatttggt
    19561 gatgagcaag ttcacatctg gcgtcgttca tacgatgtat tgcctccaaa tatggaccgt
    19621 gatgatgagc actcagctca cacagaccgt cgttatgctt cacttgacga ctcagtaatc
    19681 ccagatgctg aaaacttgaa agtgactttg gaacgtgctc ttccattctg ggaagataaa
    19741 atcgctccag ctcttaaaga tggtaaaaac gtattcgtag gagctcacgg taactcaatc
    19801 cgtgcccttg taaaacacat caaaggtttg tcagatgacg agatcatgga cgtggaaatc
    19861 cctaacttcc caccattggt attcgaattc gacgaaaaat tgaacgtcgt ttctgaatac
    19921 taccttggaa aataaaaaat tgtaagtcta gaattgattt ctaggctttt tatgttagta
    19981 tggaagtatg ataaggaata aaaaacaaga ttatgtactg gcctacaagc aaccagtttc
    20041 aaccacttac atgggttggg aagaagaagc tttaccgata ggcaatggtt ctttaggagc
    20101 aaaagtattt ggccttatag gggctgaacg gattcaattt aatgaaaaaa gtctctggtc
    20161 tggaggtcca cttcctgata gttcagatta tcagggtgga aatcttcagg atcagtatgt
    20221 ttttttagct gagattcggc aggctttgga gaagagagat tacaatctgg ctaaggaact
    20281 ggctgagcag cacctaattg ggccaaaaac gagtcaatat gggacctatc tgtcttttgg
    20341 ggatattcac attgagttca gccagcaagg tacgactttg tctcaggtga cggactatca
    20401 gagacagctg aatattagta aggcacttgt gacgacttct tatgtctata agggaacgcg
    20461 atttgaacgt gaagcttttg cgagttttcc agatgatctc ttggttcagc gttttactaa
    20521 ggaaggggcg gaaactctag attttactat agaactatcc ttgacctgtg atttggcttc
    20581 tgatggaaag tatgagcagg aaaaatctga ttacaaggag tgtaagttgg atattactga
    20641 ttctcatatc ttgatgaagg gaagagttaa ggataatgat ctgcggtttg ctagttatct
    20701 agcttgggaa acggatggag atattagagt ttggtcagat agggttcaga tatcaggagc
    20761 cagttatgcc aatctcttct tggccgctaa gacggatttt gcccaaaatc ctgctagcaa
    20821 ttatcgcaag aaactagatt tagagcaaca ggtgatagac ttggtggaca cagctaaaga
    20881 aaagggctat acccaattga aatcaaggca tatcgaggac taccaagcct tattccagcg
    20941 tgttcaattg gatttggaag ctaatgttga cgcattcact acagatgatt tgttaaaaaa
    21001 ttataagcca caagaagggc aggctttgga ggagctgttc ttccagtatg gacggtattt
    21061 attgattagt tcgtccagag actgcccaga tgctctacca gctaacctac agggagtctg
    21121 gaatgcggtc gacaatcctc cttggaattc ggactatcac ttaaatgtca atctgcagat
    21181 gaattattgg ccagcctatg ttaccaatct cctagaggcg gtctttccgg tcatcaacta
    21241 tgtagatgat ttgcgtgtct atggtcgtct agcggctgta aagtatgcag gaatcgtctc
    21301 tcaaaaaggt gaggaaaatg gttggttggt tcatactcaa gcgactccct ttggttggac
    21361 ggcacctggt tgggattact attggggttg gtcaccagct gccaatgcgt ggatgatgca
    21421 aaccgtttat gaagcctatt cattttatag ggaccaagac tatctcaggg agaaaattta
    21481 tcccatgttg agggaaacgg ttcgtttttg gaatgccttt ttacataagg atcagcaggc
    21541 gcagcgttgg gtgtcttctc cgtcttattc cccagaacat gggccgattt cgattggcaa
    21601 tacttatgac caatctctga tttggcagtt atttcatgat tttattcagg ctgctcagga
    21661 attgggactg gatgaggact tgttgactga ggttaaggag aagtctgatt tactaaatcc
    21721 tttgcaaatc actcaatctg gtcgaatcag ggagtggtat gaggaggaag agcagtattt
    21781 tcaaaatgag aaagtggagg cccagcatcg gcacgcttcc catctagtgg gactctatcc
    21841 tggcaatctc tttagctaca agggacaaga gtatattgaa gcggcacgtg ctagcctcaa
    21901 tgatcgtgga gatggcggca caggctggtc caaggctaat aagatcaatc tctgggcgcg
    21961 tttgggagat ggcaatcgag cccataaatt attggcagag cagttaaaaa catccacctt
    22021 gccaaatctt tggtgtagcc atcctccttt tcagatagat ggtaattttg gtgctactag
    22081 tggcatggca gaaatgttac tccagtctca tgcagcttat ctggtacctc tagctgccct
    22141 acctgatgct tggtcaacag gttctgtttc aggcttaatg gcacgtggac attttgaagt
    22201 gagcatgagc tgggaagata aaaaactctt acagttgacc attttatcaa ggagtggagg
    22261 agatttgcga gtttcttatc cagatattga gaagagtgtg attaaaatga atcaagaaaa
    22321 aataaaagcg aaatgcatgg ggaaagattg tatttcggtg gcaacagcag aaggtgatct
    22381 tgttcaattt tatttttaag aagatgttat aaggcagtaa tttgaaactt ccttttaata
    22441 aggatttaag aatataggca gtttccaact agttgaaaaa gcgttataat gataatagga
    22501 agtaatactc aatgaaaatc aaagagcaca aactaggaag ctagccgcag gttgctcaaa
    22561 acagtgtttt gaggttgtag atggaagctg acgtggtttg aagagagatt ttcgaggagt
    22621 ataatttgtt tgatagaggg tgggtctgat ggcttatatt gagatgaaac actgttacaa
    22681 gcgttatcag gttggggaca cggagattgt ggccaatcgt gatgtgaatt ttgagattga
    22741 aaagggggag ctggttatta tccttggtgc ttcaggtgca ggcaagtcaa cagttcttaa
    22801 ccttcttggg ggaatggata ccaatgatga aggggaaatc tggattgatg gtgttaatat
    22861 tgcggattat agttcccacc agcgcaccaa ttaccgtaga aatgatgtgg ggtttgtttt
    22921 tcagttttat aatctagttt ctaatctgac agctaaggaa aatgtggaac tggcttctga
    22981 aattgtgaca gatgccttga atcctgatca ggtcttgaca gatgtaggtc tggctcatcg
    23041 tctcaataac tttccagccc agctttctgg aggggagcaa cagcgagtct ccattgcacg
    23101 cgcggtagcc aaaaatccta aaattctcct ttgtgatgaa ccgactggag ccttggatta
    23161 tcagacgggc aagcaggttt tgaaaattct ccaagacatg tctcgtcaaa agggagcgac
    23221 ggtgatcatc gtgactcata atggagcttt ggcgcccatt gctgatcgcg tgattcatat
    23281 gcacgatgcc agtgtcaagg atgtggtgct caaccagcat cctcaggata ttgacagttt
    23341 ggagtactag catgatcaag cgaaaaactt attggaagga cttaattcag tccttcacag
    23401 actccaaggg gcgtttttta tccatcttga tcctgatgat gttgggatct ctagccttag
    23461 taggcctcaa agtaaccagt cccaacatgg aggcgacagc taatgcttat ttaacaactg
    23521 ctcaaacctt ggatttggca gtcatgtcta actatggctt ggatcaagca gaccaagaag
    23581 aactaaaaca gacggagggc gcagaggtcg agtttggcta tttgacagat gtgactatgg
    23641 ataatgggca ggatgccatt cggctgtact ccaaaccaga gcgaatttca acctttcagc
    23701 taagaaaggg acgacttcct cagtcagaca gggaaatcgc tttggccact catttgcaag
    23761 gccaatacag cgtgggacag gagattagtt ttaaagaaaa agaagagggt cattcctctt
    23821 taaaagacca tacttatacc attactggtt ttgtggattc ggctgaaatc ctctcccagc
    23881 gagatatggg ctacgcagga agtggaagtg ggactctgac agcctatggg gtgattttac
    23941 ctagtcaatt tgatcagaaa gtctacaata tagctcgttt gaaatatcaa gatttagcag
    24001 gtttaaatgc cttttcatca gcttatgaag aaaaatccaa gcaacatcag gaagaccttg
    24061 aacaaacttt atcagataat ggcaaggtac gtctgcaact tttgaaaaaa gaaggacaag
    24121 agtctctaga caaggggcaa gagactcttg acaaggctca gactaatttg caggaaggca
    24181 agcgtcgttt agcagctgct caagctcgta tacaggctca agaaagtcaa ctagccttgt
    24241 ttcctcaagc tcagagagag caggctagtg ctcaacttac ccaagccaag caggaactgg
    24301 gcaaggaaga ggacaaacta aagcaagctg aacaaaatct agcccaagaa aaggaaaaat
    24361 tagaaaaaca tcagcaagtc ttggatgatt tggcggagcc aaggtatcag gtttataatc
    24421 gtcagaccat gccaggtggt cagggctatc ttatgtatag caatgcttca tccagtattc
    24481 gagcagtggg caatatcttt cctgtggtac tttatgccgt agcagccatg gtgaccttta
    24541 cgaccatgac tcgctttgta gacgaagagc gaactcatgc agggattttt aaggccttgg
    24601 gttatcgtag taaggatatt atcgccaagt ttctccttta tggactagta gctgggactg
    24661 tcggaacggc tctaggtagt atacttggtc attatttgct agccagtgta atttcaagtg
    24721 tcattacaaa aggcatggtg gtgggagaaa cccagattca gttctattgg acctatagct
    24781 tactagctct tgtcttgagc tggttggcga gtgtgttacc agcctatctg gtggcttgga
    24841 gggaacttca tgacgaagca gcccagcttc tacttcctaa acctcctgtc aaaggagcta
    24901 aaatcttatt ggagcgtatc ggttttatct ggcgtcgtct cagttttact cataaggtaa
    24961 cagcccgcaa catctttcgt tataagcaga gaatgttgat gacaatcttt ggtgtggcag
    25021 gttctgtagc tctgctcttt gcaggtttgg gaatccaatc ttctgtagca ggagttccgt
    25081 ctaaacagtt tcaacaaatc caacagtatc agatgcttgt ctctgaaaat cctagtgcga
    25141 ccaatcagga caaggtagag ctagcagaag tgttgaaagg gcaggagata ctagcctacc
    25201 agaaaatcta ttctaaaacg ctagacaagg atttcaaggg cagggctggt cttcaaaaca
    25261 ttactcttat gatgatagag aaggaagatt tgactccctt tatccatctt caacatcatc
    25321 agcaggagct gacattaaaa gatggcatcg ttattacagc taaactcgcc cagctggcag
    25381 gtgtcaaggt tgggcagact ttagaaattg aaggtaagga actaaaggtc gctgctatta
    25441 ctgagaacta cgttggtcac tttatttata tgagtcaggc tagctatgag caactttacg
    25501 gacagctacc ccaagccaac acttatctgg tctcattaag ggataccagt gcaactagta
    25561 tcgaaagtca ggcgggcttg cttatgaatc aatctgcggt gtccagtgtt gtccaaaatg
    25621 cttcagccat tcgactcttc gactctatcg ctagctcact caatcagacc atgaccatct
    25681 tggtcatcgt atcggttcta ttagctattg tcatccttta caatctgacc aatatcaacg
    25741 tagctgagag aatccgtgaa ctctccacta tcaaggttct cggttttcat aataatgaag
    25801 tcaccctcta catttaccgt gagacgattg tgctgtccct tgtgggaatc gtacttggtc
    25861 tggtagctgg tttctattta caccaatttt tgattcaaat gatttcgcct gcgactattc
    25921 tcttttatcc gcaggtaggc tgggaagtct atgtaatccc agtggcagca gtaagcatca
    25981 ttttgacctt gcttggtttc ttcgtcaatt attatctgag aaaggttgac atgttagaag
    26041 ccctgaaatc tgtagagtaa ggtagttatt tttagctgat tgaacttcta tttactaata
    26101 ttcaaaaatc ctccgtttca aagagcaggg aactctttgt gacagaggat tttttctata
    26161 gggctttagc agctgcaatt gcggcttcga agtttggctc agaattgata ttatccacgt
    26221 attcaacgta gcgaatcgta ttgtcagtat cgaggacaaa gactgcgcgt gctaataggt
    26281 gccattcgtt gatcaagagg gcataatcgc gtccgaaaga atggtcaaag tagtctgaaa
    26341 gcataatggc attgtcaagg ccttcagcac cgcaccaacg tttttgagca aaaggtaggt
    26401 ccattgaaac agtcaatacg accgtgttgt ccagtccagc caattcttca ttaaaacgac
    26461 gtgtttgagt tgagcagatg cctgtatcga tagaaggaac gacactcaag acttttttct
    26521 tgccatcaaa atcagccaga gattttttag aaagatctgt tgtagtaaga gaaaaatcaa
    26581 gcgccttgtc gccgacttgt agttgtttac ctgtaaagct cacaggattt ccgagaaaag
    26641 ttaccatagg atactccaat cttttttctt ccattgtatc tgaaacagtc agaattttcc
    26701 aatgatttga ccggaaatgt gtgcatagaa aaaacgccag ctcacatgag aatgacgttt
    26761 ttcagaggct tattttgcca atccttcagc aatcttgtca aggttgtatt tcatcatgct
    26821 gtagtagctg tcgccttctt taccttgttc tgcgatagag tcagtaaaga tttgtgcgta
    26881 gattgggatg tttgtgtctt gagaaacagt tttcattgga cggtcatcca cacttgattc
    26941 tacaaagagt gatggaactt ttgtttggcg aagtttttca accaaggtct tgatttgttc
    27001 aggagttcct tcttcttcag tattgatttc ccagatgtag gcacttggga caccataggc
    27061 tttagagaag tatttgaatg ctccttcgct ggttacaatg agtttctttt cagcagggat
    27121 cttattaaat ttatccttac tttctttatc aagtttgtct aacttatcag tatattcttt
    27181 gagatttttt tcatagaatt ctttattgtt agggtctttg gcgctcaatt gtttggcgat
    27241 atttttagca aaaataatac cgttttcaag gttaagccaa gcgtgtgggt cttcttttcc
    27301 tttttcattt tgaccttcaa ggtagataac atcaacgccg tcgctgactg cgaagtagtc
    27361 tttgttttca gttttcttgg cattttctac caattttgta aaccaagcat tgccacctgt
    27421 ttcaaggttg ataccgttat agaaaatcaa atcagcctca gaagttttct taacgtcttc
    27481 aggaagtggt tcgtattcgt gtgggtcttg cccaatcgga acgatactat gaaggtcaat
    27541 tttgtcacca gcaatatttt tagtaatatc agcgatgatt gagtttgtag caacaacttt
    27601 tagtttttga ccagaagttg tatctttttt tccgctagca catgctacaa gaatgattgc
    27661 agaaagaaag agaacgagta atgtacctaa ttttttcatt agatcctcca atttattagg
    27721 gctttgcccc ttattttaac aaatgtttat ttttcagttt caaatatcgt tgtttgggag
    27781 cgataaagaa gctaatgaga aagaaactag cagctgtaag cacgatacta gaacctgccg
    27841 caacattaaa actatagcca ataaagagtc ccaaaactga agcagtagct ccgaaggttg
    27901 aggaaaggaa aatcatgctt ttcagactat tagcatacag ataagcagtt gcagctgggg
    27961 taatcagcat ggctacaatc aggatagttc cgacactttg catggctgtc acagacacga
    28021 gagtcaggag taccatgaga aggtagtgat agaaattgac aggcattccc atggctttag
    28081 ccaagagttc atcaaaggaa gttatcaaga gttgcttgaa gaaaatccag attaacaaga
    28141 ggatggctgc ccccacaccc atagtaataa acatatccgt atcttggacg gccagaatat
    28201 taccaaaaag gatatggaaa aggtcagttg aacttttagc gacaccaatc aagatgatac
    28261 cgagggctaa gaaagaagaa aaggtaatgc cgatggcggt atcgcttttg ataatcgagt
    28321 ttcctttgat gtaggtaatg atgatggcag ctagcaatcc aaagacaatg gctccgataa
    28381 agaagtcaag gcccaagatg aaggagaggg ctacacctgg taagacagca tgtgaaatgg
    28441 catctcccat gagtgacatc ccgcgtagaa taatgaaaca tcccacggct ccagctacaa
    28501 tcccgacgac aatagctgtt atcaaggcat tttgtaggaa atggaatttt tgcaatccat
    28561 cgataaattc tgcaatcata ggtcacctcc attgaaaaag agttgattac cgtaagcttc
    28621 ttttagattg gtttcggtaa aagtttcttt tgttggacca aaggcaatca cttctcgatt
    28681 gacaagtaag acttgatcga agtagtgggg aatcttgctg aggtcgtggt gaacgatgag
    28741 aaccgtcttc ccagcttttt tcaaatctct cagcgtattc atgatgattt cctcactgac
    28801 agagtcaatc ccagcaaagg gttcatccaa gaggatatag tcggcttcct gcaccaaaca
    28861 tctggcaatc aagacccgct ggaattgacc tccagacagt tgactaattt gacgttcagc
    28921 gtagtcagct aggccgacga tttcaagggc ctcttgcact ttcttccaat gtttagcctt
    28981 taaacttcga aagagaggaa tagagggaaa tagtcctaac gagacgcatt ccttgacctt
    29041 gatgggaaag ttgtagtcga tattgatttt ttgttcgaca taggcaattc ggtgtaagga
    29101 ttttttaact tccttgtcat cgagaaatgc ctgaccttga tgtgggataa ttcccagcat
    29161 accttttaat agtgttgatt tcccagcgcc gtttggacca atgatgccgg taattgttgg
    29221 tccatggagc actagtgaaa tatccttaag tgccaacgtt tctttgtagg agacactgag
    29281 gttttcgata cgtatcataa acttgtattc ctcctgtctc ttaatataca ttaaaaaaaa
    29341 aattaagtca agttaatttt tgaaaaaatt aaaataataa ctgaaaaata gattctaaag
    29401 ataactttca ggataaattt ctaaattata aaacgcatag tatcaagtgt aaaaaacttg
    29461 gaattatgcg ttttatcatg gaaagatttt ttataatagc taaaaaataa tagttaaatg
    29521 gttattaatt gcattcccta gtgatttttg ttaagataaa tgcaaataca aatgaaagcg
    29581 agaacaagat gacacgttat caagatgatt tttatgatgc tatcaatgga gaatggcaac
    29641 agacagctga aatcccagca gataagtctc aaacaggagg ttttgttgat ttagaccagg
    29701 aaattgaaga cctgatgctg gcgacaacag acaagtggtt agcaggtgaa gaagtgcctg
    29761 aggatgctat cttggaaaac tttgtcaaat accaccgcct agttcgtgat tttgacaaga
    29821 gagaagctga cggtatcaca cctgtcttac cactccttaa agaattccaa gaattggaaa
    29881 cttttgcgga ttttacagct aaactagcag agtttgagct tgcaggaaaa ccaaacttcc
    29941 ttccttttgg tgtatcgcca gactttatgg atgctagaat caatgttcta tgggctagcg
    30001 ctccaagcac aatcttgcca gatacgacct actatgcaga agaacatcct cagcgcgaag
    30061 agctcttgac tctttggaaa gaaagcagcg caaatctcct caaggcttat gatttctctg
    30121 atgaagaaat tgaagacttg ctagaaaaaa gacttgaatt ggaccgccga gttgcggcag
    30181 tggtgctctc taatgaagaa agttcagaat atgctaaact ctatcatcca tattcttacg
    30241 aagatttcaa gaaattcgcg cctgccctac ctttggatga cttcttcaaa gcagttattg
    30301 ggcaattacc agacaaggtt attgtagacg aggaacgttt ctggcaagca gcagagcaat
    30361 tttacagtga ggaagcctgg tctctcctta aagcaacctt gattttgagt gttgtcaatc
    30421 tttcaaccag ctatttaaca gaggatatcc gtgttttgtc tggtgcctac agccgtgccc
    30481 tttctggagt tccagaggca aaagataagg tcaaagcagc ttatcatcta gcacaggaac
    30541 ctttcaagca agccctgggg ctttggtacg cccgtgagaa gttctctcca gaagccaagg
    30601 cggatgtgga gaaaaaagtg gcaaccatga ttgatgtcta taaggagcgt ctgcttaaga
    30661 atgactggct cactccagaa acctgtaaac aggctatcgt gaagctcaat gtgatcaaac
    30721 cttatattgg ctatccagaa gaattgcctg cacgttacaa ggataaggta gtgaatgaaa
    30781 ctgccagtct ttttgagaat gctctagcct ttgcgcgtgt ggaaatcaag cacagttgga
    30841 gtaagtggaa ccagcctgta gactataagg aatggggcat gcctgctcat atggtcaatg
    30901 cctactacaa tcctcagaag aacctgattg tctttccagc ggccatttta caggcgcctt
    30961 tctatgactt gcatcagtca tcttctgcta actacggtgg tattggggca gtgattgccc
    31021 atgaaatttc ccacgccttt gatactaacg gggcttcctt tgacgaaaat ggtagcctca
    31081 aggattggtg gacagagagc gactatgctg ccttcaagga gaaaacacaa aaagtcattg
    31141 accaatttga tggacaggat tcttatggag caaccattaa cggtaaattg actgtatcag
    31201 aaaacgtggc tgacttggga ggaatcgcag cagcgcttga agcagctaag agagaagcag
    31261 acttctcagc agaagagttc ttctacaact tcggtcgcat ctggcgcatg aaaggtcgac
    31321 cagaatttat gaaacttttg gctagcgtcg atgtgcacgc accagccaaa ctccgtgtca
    31381 atgtgcaagt accaaacttc gacgatttct ttacaaccta tgatgtcaaa gaaggagacg
    31441 gaatgtggcg ttcaccagag gagcgcgtga ttatttggta atacaaaaaa tctagtcatc
    31501 agaaaaaagg catccaatca cgtgtgaaaa ccttgatagg atgccttttg taatagaaag
    31561 atttgctgga tagtttactt aaatttcgat tgtcatcatc ttgctagaaa aagggattga
    31621 aggccttttc gtgagcgata gtagtagctg gaccatgtcc tggatagaca tcgtagtttg
    31681 gtagggtgaa gagttgggtc tggatactat gaaggagttg ctccatgcta ccagtcggaa
    31741 ggtcggtccg tccgatagtt tcgcggaata gagcatctcc cgtcaagact aggtgagcat
    31801 caggaaagac tagggaaaca ccaccgatag agtgccctgg ggtcggtaga accttaaaac
    31861 gaaattcctc tagttggtat tcttcgtgaa agacaaaggt gtgttctgca ggttttgtga
    31921 ccacatctgc catatcatcg tggcgaggga gaccggagag attatcgaca ggagtgtaga
    31981 gccagctggc ttcgctctct gcgatataga caggaggatt gccaaacgtc tcgcgaacca
    32041 agtccagact catgatatgg tcataatggg cgtgggtcaa gagaatagca cagatcggtt
    32101 tgttgatctt ctcgattgtc tgacgaatgg cttcccaatg actaccagga tcgacgacga
    32161 tgaggtgctt ttcgccttct agataatagg tattttcata ggcaacagga ttcacggttt
    32221 tatggatttt catactagct ccaatctcaa agaatgtact aatactcttc gaaaatctct
    32281 tcaaactgcg tcaactttat ctgcaacctc aaagcagtgc tttgaacagc ctgcggctag
    32341 ctttctagtt tgctctttga ttttcattga gtataaatta agtatagcac agttagggag
    32401 aataggtaag gatttaaaat gaacttaaaa accagcatga ctagatgaag acaggctggt
    32461 tatcaattta tcaatattgg aaaggtgtca attgcccttc ataggttgca taaactccgt
    32521 cattagcttg tttgatagac gtgatgttta gagtgctacc gtagttggct tctcgtttgt
    32581 cgctatattc actataagtt atcctactgg gcaggttctg gtcgttagca tagtattgaa
    32641 cgactgtttt ttatagcttt gctggctgaa taggaacagg cagcaaactc ctaacattag
    32701 gagagcaaag atagaaatag cagttttttt cataggtatt ctccttataa actaaaggat
    32761 agaagaaagt atcttgagga tttttatact cttcgaaaat ctcttcaaac cgcgtcaacg
    32821 tcgccttgcc gtatatatgt gactgacttt gtcagtctta tctacaacct caaagcagtg
    32881 ctttgagcaa cctgcggcta gtttcctagt ttgctctttg attttcattg agtattaatt
    32941 ccaaaatcca gtcaaggtac cagtgtatgt gatacgagtt ccaccgttta tctttcgtat
    33001 cttcaatgga attggggaaa tcagaaagtc tttgggttac aatatggttt ccgttcttct
    33061 atctctcctt ctatcaccta agtttaaaat ctaacttcgt agtatattat gggccaataa
    33121 ttcagaatta tcaagcaatt ttgaattatt tttttcttat tctgttccaa tctagttttc
    33181 atattcttaa aaaagtgata aaatggtagg aagaattgga gagtagagat gccgaaagaa
    33241 gtgaatttaa caggcgaaga agttgtcgct ttaaccaaag aatatttaac ggaagaggat
    33301 gttcattttg tccataaggc cttggtctat gctgttgaat gccacagtgg tcaatatcgc
    33361 aaatcaggcg agccttatat cattcaccct atccaagtgg caggtatttt agctaagcta
    33421 aagctggatg ctgtaacagt agcttgtgga ttcttgcatg atgtggtgga agatacagat
    33481 gcgaccttgg acgatttgga aagagagttt ggtcctgatg tgcggatgat tgttgacgga
    33541 gttaccaagc ttggcaaggt cgagtacaaa tcgatcgagg agcaattagc ggaaaatcat
    33601 cgcaagatgc tcatggccat gtctgaggac atccgcgtta ttttggtcaa actgtctgac
    33661 cgcttgcaca atatgcggac cctgaaacat cttcgaaaag acaagcagga gcgtatttcc
    33721 aaagaaacca tggaaatcta tgccccgctt gcccatcgtt tggggatttc cagtgtcaaa
    33781 tgggaattag aagacttgtc tttccgttat ctcaatccaa cggagtttta caagattacc
    33841 catatgatga aggaaaagcg cagggagcgt gaggccttgg tggatgaggt agtcacaaaa
    33901 ttagaggagt atacgacaga acgtcacttg aaagggaaga tttatggtcg tcccaagcat
    33961 atttactcaa ttttccgcaa aatgcaggac aagagaaaac ggtttgagga aatctatgat
    34021 ctgattgcta ttcgttgtat tttagatacc caaagtgatg tttatgccat gcttggttac
    34081 gtgcatgaat tttggaaacc gatgccaggt cgcttcaaag actatatcgc caaccgcaag
    34141 gccaatggtt atcagtctat ccatacgact gtttatggac caaaagggcc gattgaattc
    34201 cagattcgaa ccaaggaaat gcacgaggtg gctgagtacg gggttgcggc tcactgggct
    34261 tataagaaag gtataaaggg gcaagttaac agcaaggaat cagctattgg aatgaactgg
    34321 atcaaggaga tgatggagct ccaagaccag gctgatgatg ctaaggaatt tgtggactct
    34381 gttaaggaaa actatttggc tgaggagatt tacgttttta ccccagatgg agctgtccgt
    34441 tcccttccca aagattcagg accgattgat tttgcctacg aaatccatac caaggtcggt
    34501 gaaaaagcaa ctggtgccaa ggtcaatggc cgcatggttc cactgacaac caagttaaag
    34561 acaggggatc aggttgaaat tatcgccaac ccgaactcct ttggacctag ccgtgactgg
    34621 ctcaatatgg tcaagactag caaggcgcgc aataagattc gccagttctt taaaaaccaa
    34681 gataaggaat tgtctgtcaa caagggtcgt gagatgctga tggctcagtt ccaagaaaat
    34741 ggctatgtgg caaataaatt tatggacaag cgccacatgg atcaagttct gcaaaagacc
    34801 agttacaaga cagaagactc cctctttgcg gccattggtt ttggggaaat cggtgcgatt
    34861 accgtcttta accgtctgac tgaaaaggaa cgccgtgagg aagagcgtgc caaggccaaa
    34921 gctgaggcag aggagcttgt caaaggtggc gaggtcaagg ttgaaaataa agaaaccctc
    34981 aaggtcaagc atgagggggg agtggttatt gaaggtgctt ctggtctcct agtgcggatt
    35041 gctaagtgtt gtaaccccgt gcctggtgac gatattgttg gctacattac caagggtcgt
    35101 ggtgtggcta ttcaccgtgt ggactgtatg aacctgcgtg cccaagaaaa ctacgagcaa
    35161 cgtctccttg atgtggaatg ggaagaccag tactctagct caaataagga gtatatggcc
    35221 catatcgata tctacggtct caaccgtaca ggactgttga acgatgtact gcaagttctt
    35281 tcaaatacaa ccaagaatat ttcaacggtc aatgcccaac caaccaagga tatgaagttt
    35341 gctaatatcc atgtgtcctt cggtattgcc aacctctcta cactgaccac ggttgtcgat
    35401 aaaattaaga gtgtgccaga agtttactct gtcaaacgga ccaacggcta gttgtcctag
    35461 ctcttactag aaaggctatt atgaaaatca ttatccaacg ggttaaaaaa gcccaagtga
    35521 gtatagaagg ccagattcag ggaaaaatca atcagggatt tttattgctg gttggtgttg
    35581 gaccagagga ccaagaggaa gatttggact atgctgtgag aaaactggtc aatatgcgga
    35641 ttttttcaga cgcagaaggc aagatgaacc tgtctgtcaa agatattgaa ggagaaatcc
    35701 tctctatttc tcagtttacc ctctttgcgg atactaagaa aggcaatcgt ccagccttta
    35761 caggggcagc taaacctgat atggcatcag acttctatga tgctttcaat caaaaattag
    35821 cgcaagaagt gcccgttcag acaggcatct ttggagcaga tatgcaggtt gagctggtta
    35881 ataacggacc tgttaccatt atcctagata ctaaaaagag atgagaaaga ccaagcccag
    35941 taggcttggt ctttctcatc tatcataaaa tactccaaaa agaaatcggt tcttgatatg
    36001 cttgggggaa gtctcttttc aggctttggc agatgcgata ggaagggatg agatgtccta
    36061 ggatgaggag agttccctga aggaaaagtg gaatatcact taaagacacc aaggagagaa
    36121 tcatcaggct atcccatagg agaatttgta aagcaaaacg ccagaatctc ggtctaatct
    36181 gggaatagag ttaactaaag tggtcacaaa gttggtttga aagataggtc aatagcacag
    36241 gatgaaagaa aatgagccaa ccgctataaa tcaaaatcca gtcccaagta agctcccttt
    36301 taaatgtcaa aaatgccagc atgattccat caatgacaag gagttgaatg gttttgatga
    36361 atatgatttt tttcatgcta aaacctcctt ttccttagag acactcccat aaagagatgt
    36421 gtttgtcgta aaagtctgga tagttttctc ctaggtagtc agctgtcata taataaaaag
    36481 tagaatggcg agcagtgatg attggcataa gagatgaaaa acgctgagaa ctaatgaagg
    36541 ctaagagaac aaaaaataga aaatcaatgg tgagaatccc taaatagtag aagcgaatct
    36601 ttttattgat ttgaggataa atctcagcga attgatgttt gagtcggacg accaagagaa
    36661 atagcaggag tccttgatca aggaagaaaa ttaaaccaga gagcaagagc cactcaaaag
    36721 atgtctcgag tctagtgata aaaaatgcaa atagtagcaa atcaataact gcaattgcta
    36781 taaaatggat tctatagagt tttttcatga caagaccttc ctcttttatc taacttcatt
    36841 ctactccaaa agaatgggag ttacaactaa aatgataaaa atagcagagg ggagattctc
    36901 ttaagttggc tagtattctt tatttgagtt tccttctatt atctaacttc ttcatcattc
    36961 cagacaaata aagctccgat tgcattgagg atataaaaga tgtatttacc gatattggcg
    37021 aagtttcctt gaataccagc ttttgtcagc tgaacgaaat tgtaaatcaa ccaaaagccc
    37081 cactgagttg ttagtttcaa tgcattcaaa gcattggcaa tgagggacag tgcaaaggca
    37141 atagttgtta cgtaggcaag gagattcatc ttgcccccat atccgatata gttggtcaca
    37201 aaggcaaaga ggaaggcgat gatggaaatg atgatgggcg ccaattttac ctgtttttgg
    37261 ctcatttggt tgggtctgcc ttcttgcgaa gctttccatt tctttatagc aaaggtataa
    37321 atgaggaagg tgacgggata ggtaatgatg gccgccttat ttccaaggat ataatcaata
    37381 gcaccggaca aaatggtatt aacaatacca aagtaatttc cccatttgct taatttcccc
    37441 gtgaaacgag tggacaacat ggaaatccca acgttagtta cggaaatcaa tccaaagggt
    37501 acaagagctg tccatagtcc ccagtctaca aatttatgga ggcgtgagtt gagataacca
    37561 gatgcaatcg caatcccaac gaccaaagca accccgaaga ggtcaaacta tttagatgta
    37621 gcaaaaattt ttagtgattt tttcataggt taaactacct ttcttttttt caaatattct
    37681 cccatttttt tcaaatattc tcacaccaaa tgaaagtaaa ataaaatgat agaaataaaa
    37741 ccctgaaaat aaaactttca gggttggtag gggacttgag aaattagttg attttctcga
    37801 catagagttg ttttgcaatg tccatattca cttgtaaatc ctcgtcgtta ctgaggatag
    37861 tgaaggtatt gctgaagcca tcaaactgct tgacttggag ctggtcaccg atgtgaagtg
    37921 agtgcttgtc cagataatgg agaatgtcaa aactatcgtg cacccgagtc aggcggtagg
    37981 cgccagcttc cttgatatca gctagtggga ggttattgat ttcaacgagg agttctccct
    38041 tggcaggaat agttcctccg tgggggcagg ttttagggaa acctagcagt ttatctagtc
    38101 tttccacgaa caggtcagag acagtgtgtt ccaagacctc agcttcctcg tgaatctggt
    38161 cacttgtata gtctaaatga tgaactagaa aaacttcaat caagcggtgc ttacgataga
    38221 gctcagagac cagtttgaga ccgaggtcag tcagtagata gccacattcc ttgtccttta
    38281 ggatgagatt ttcacttttc attcgtttga tcatttcagt tacggcaggg ggagagactt
    38341 gcatgcgagc cgcaatttcc ttgttggtaa tcttatgcag gtctatgcca atttcataaa
    38401 tacattttag atagtcttct ttgtttgggg tcattcgttt cctcttgtct aatatcttta
    38461 tctagtatat caaaaaaagc tagatttctc tagctgtgac tttttatgtt atacttattg
    38521 caagagaaaa aacgaggaga tggatatgac gaaaatagct cttctttcag atattcatgg
    38581 aaataccacc gccttggagg ctgttttggc agatgctcgg cagataggag tggatgaata
    38641 ctggcttttg ggagatattc tcatgccagg gacagggcgt agaaggattt tggacttgtt
    38701 ggatcaacta ccgattacgg ctagagtttt gggaaactgg gaagacagtc tttggcatgg
    38761 tgtccgtaag gaattggata gtactcgccc cagtcaacgc tatctcttgc gccagtgcca
    38821 gtatgtttta gaggaaattt ccctagaaga aattgaagtg ctccacaatc aacctcttca
    38881 aattcatcgt cagtttgggg atttgacggt gggaattagt catcatctgc ctgataagaa
    38941 ctgggggcga gagttgattc atactggcaa acaagaggat tttgaccgct tggtgactca
    39001 tcctccctgt gatattgctg tttatggaca tattcaccag cagttgcttc gttacgggac
    39061 tggtgggcaa ttgattgtca atccgggttc gattggccaa cctttctttt tggacgctca
    39121 gctacggaag gacttgcggg cccagtatat gattttggag tttgatgaca agggcctggt
    39181 agatatggac ttccgacggg tagactacga tgtggcagct gaattgcagc tggctaaaga
    39241 cctgagactt ccctattttg aggtttacta tgaaagtctg gtcaatggga tccaccatac
    39301 tcatcatcag gaatttttga gagaattggc tcagaaagag ggctgcgacc gggagttaga
    39361 cgactggttg aaaagtggta acgattgaca ttacaactaa aaagggtata ataatagaaa
    39421 aagaagagta gaggcagggt agcctcgtaa aaaggagaag aggatgcaaa ttccaagtag
    39481 atttaccatt gcgactcata tgctgataat cattgccctc gaggggaagg aaagcaaggt
    39541 gaccagtgat tttctggctg ctagtgtcgg ggtcaatcct gtcattatca gaaagatctt
    39601 gtcccagttg aagaaggcag agctgatttc agtagcgcgt ggaacgggcg gaacagagat
    39661 tgtcaaggac cttaaggata ttagtctttt agatgtttat caggcggtcg aatgtcttgg
    39721 taagacaggt caactcttca gtttccatga caatccgaat ccaaattgcc ctgtaggagc
    39781 tcatattcat gatgttttgg atcaaaaatt ggagagaatt cagttgacta tggaggcaga
    39841 acttggtcaa accagtctag aaaaagtcgt ggccgatgca gagagtcaga tgaaggatta
    39901 agagggataa atgccctctt ttttctttgg ataattatga taaaatgtag ctattaaagg
    39961 agtaagaaat gtatctcgtt ataggaattg tattagcctt tatagtgtca ttttggaagg
    40021 ataatagaag tttgtggaat ccagtattat ttttattatc cctcatttca agttattttt
    40081 atctatccta ccttttttat aaaaatggat acgagaatgt tcagttagct ttttatgtat
    40141 ttgcctttgt cttacttcct tttttgcttt ttctaagtgg catcttttta atctataatg
    40201 gggtcatttt gttgaagcga gagggacgtt ccaagcccca ttatctctca atgctctttg
    40261 atttttattg actattagat tctatttaaa aagacttaga atgcacatga aagtatgtat
    40321 tctaggtctt ttgttttgta tacatttaat tttcaagagt attatctatt tttccatgcc
    40381 tggtaacgag tatcaatcaa taactgggta aggcgaccca aggtttctct ttcttctgga
    40441 gtgagggatt gagcttggac aaagagatga tgaatgtatt caagcgcctt taaggaagcc
    40501 aagtcattga tagagctaat accagcctca agaatagtgc caaagaagag gtcgaagtag
    40561 agctgaagtt cttcgtcagg gactgtggcc acccattctt taaaggttgt gtctacttgc
    40621 tggctatcac tgttggtctt atccagttgg acgaagtgct tgtcctcaat ctgccaacta
    40681 aaggtatcgt gctgggcgat gccacccagg gcagtactct gaacgatgat ttggtgagca
    40741 ggaatttcca gcatcatacc gataatggaa ccttgtggaa tgaatatctt gcttctatcc
    40801 attatccttt gataacccgc agtctgtgtc aattcttgat ggagaccagg tgcatcaaat
    40861 gtataaactg ctgtgatctg attttgcaaa ctttgctcaa tttggctagc agcatagata
    40921 gcgagatttc ctcccttgga atgcccagct agaataacct tttgcttagg atgatgggca
    40981 aaaaagttct ttaaatagcg aagggcgtgc ttttgagcag gaatttcctt catataggtc
    41041 aggtggaaat cttccttcca gccaatgata ctgtcatctg tcccacgaaa gacaatcaga
    41101 taggtatcga ggctgacacg ataagtcata gccgcaaatt gcttttgcag ttcagggtcg
    41161 atgtcgttga taaaatggga gagtttgcaa tttttgaagc gcttgtgttg agccaattca
    41221 tctaataatt gaaggcgatt tttgctagta agcatggtgg gatctcttgg aacctgaggt
    41281 gctagatcta aaagacgttg aggaagtgtg gagaccagat tatcaaagga gaggtaggtg
    41341 atttctatta aggttagaat gtctaactca ttcaagggaa ggtcgtaata agaatcatat
    41401 gcgacatctt tcagatagtc aaaaatattg gccataagag ctcctttctt tttatttatc
    41461 ttagcaaatt tccctcaaat tagctagtag cattgcctgt ttgtactggc taaaaacagg
    41521 ctatttcaaa ttcagtttca gaccatctgg catggaaaaa tctgttataa taatggaaaa
    41581 ggagaagcgc atgcacaaga ttttattagt ggaagatgat caggtcattc gtcaacaggt
    41641 cgggaaaatg ctctctgaat ggggatttga agtggtcctg gtagaagact ttatggaagt
    41701 tttgagtcta tttgttcagt cggaacctca tctggtcctc atggatattg gtttgccctt
    41761 gtttaatggt tatcactggt gtcaggaaat ccgcaagatt tccaaggtac ctatcatgtt
    41821 tctttcttcg agagaccagg ctatggatat tgtcatggca atcaatatgg gggcggatga
    41881 ctttgtgacc aagccttttg accagcaggt tcttttagct aaggttcagg gcttgttgcg
    41941 tcgttcctat gagtttgggc gtgatgagag tttgctggaa tatgctggtg ttatcctcaa
    42001 taccaaatcc atggatttac attatcaagg gcaagtcttg aatttgacca agaatgaatt
    42061 ccagatttta cgcgtgttat ttgagcatgc aggcaacatc gtagcacgtg acgacctgat
    42121 gcgggaactt tggaacagtg actttttcat tgatgataat accctctctg tcaatgtggc
    42181 tcgtttgcgt aaaaagttgg aggagcaggg attggtagga tttatcgaga ccaagaaagg
    42241 aatagggtac ggattgaagc atgcttgatt ggaaacaatt ttttctagcc tatctgcgct
    42301 cccgtagtcg tctttttatc tatctgcttt ctttggcatt tctggtctta ctctttcagt
    42361 ttttatttgc cagtctagga atttacttcc tctacttttt cttcttgtgt tgctttgtaa
    42421 ccatcttatt tttcacttgg gatatattgg tggaaacgca ggtctatcgc caggaacttc
    42481 tctatggaga gagggaagcc aagtctcctt tggaaatagc tttagcagaa aaattagaag
    42541 cgcgtgagat ggaactctat cagcagaggt caaaagcaga aagaaaactg acggatttgc
    42601 tggattacta taccttgtgg gtccatcaga taaagacccc cattgcagcc agtcaactct
    42661 tagttgcaga agtggtcgac cgccaactga agcagcaact agaacaggaa attttcaaaa
    42721 tcgactccta taccaaccta gttttacagt acctgcgttt agaaagtttc catgatgatt
    42781 tggtcttaaa gcaggttcaa attgaggact tggtcaagga aataattcgt aaatatgctc
    42841 ttgtctttat tcaaaaaggc ttaaatgtca atctacatga ccttgataaa gaaatcgtga
    42901 cggataaaaa gtggctgcta gtggttattg agcaaatcat ctcaaacagt ctcaagtaca
    42961 ccaaggaagg tggtctggag atttatatgg atgaccaaga gctttgtatc aaagatacgg
    43021 gaatcgggat aaaaaacagt gatgtcctcc gagtatttga acgtggcttt tcaggataca
    43081 atggccgttt gacccagcag tcctctggac ttggccttta tctatctaag aaaatttctg
    43141 aagaactggg gcaccagatt cgtatcgagt ctgaggtcgg aaaaggaacg acagtgcgga
    43201 ttcagtttgc tcaagtgaac ttagtccttg agtaaaaata gatgagaaaa agcctccaga
    43261 ttggaggctt ttatgctaga ctcttatttc tcaacgcgtg atttgttggc gatatcagct
    43321 aggatagctt gaacaaaatt atcaactgag acagtttgtg tttctttttg gccgtagcga
    43381 cgaacgttga ctgtttcgtc ttccatttct ttgtctccaa caattaattg gtaaggaatc
    43441 ttgctggttt gtgaagcacg aatcttgaac tgcatttttt cattgcgctc atctacgtct
    43501 gcacggacac cgcggtcacg gagtttcttg gccacttccc aagcgtagtc cacgtgtttt
    43561 tcgttagata ctgggatgag ggttacttgg tgtggtgcca gccatgttgg gaaggccccc
    43621 ttgtagttct caatcaagat agctgtgaag cgttccatag ttgagataac cccacggtgg
    43681 atcatgactg gacggtgatc ttcgccatca gctccgatgt atttgaggtc gaagcgttct
    43741 ggcaacaaga aatcaagttg gatagtagaa agggtttctt cttttccaag ggcagtttta
    43801 atctggatat ccaattttgg tccgtagaag gctgcttcac cttcggcttc aaagtagttc
    43861 acgcccattt catcaagagc tgcacgaagc atggtttggg cattttccca catctcatcg
    43921 ttatcaaagt acttatgagt atcttgaggg tcacgaagag agaggcggaa gcggtagtca
    43981 gtcaagttga agtcttcata aacatcgata atcaactgaa gggcacgttg gaattcttct
    44041 tggatttgtt ctggagtaac gaataggtga ccgtcgttga gtgacatttc acgtacacgt
    44101 tgaaggccag tgagggcacc agatttttcg taacggtgca tcataccgat ttcagcgata
    44161 cggattggca attcacggta agagtgaaca tggtgtttga aaacttggat gtggtgcgga
    44221 cagttcattg gacgaaggac aaattcttcc ccgtcaccca tgtccatggt tgggaacatg
    44281 tcttcttggt aatgatccca gtgaccagaa gtcttgtaaa gctcaacaga agcaagtggt
    44341 ggagtgtaga cgtgttggta gccagaagcc aactctttgt ttacgatgta gcgttccaat
    44401 tcacgacgga tagtcgcacc atttggcaac cagaatggca aaccttgtcc cacttcttgt
    44461 gaaattataa agaggtcaag ctctttacca agtttacggt ggtcacgttc cttagcttct
    44521 tcacgcattt gaaggtagtt tttcaagtct ttcttgtcaa accaagctgt accgtagata
    44581 cgttgcatca tagcgttgtc gctgtttcca cgccagtacg caccagctac atggagaagg
    44641 tggaagattt ggatacgacc tgttgatgga acgtgaggtc cacggcagag gtctacatat
    44701 tcaccctgac gatagatagt caaaccgcct tcgtcttctg agtgttcttc aatcaattcc
    44761 aacttgtaag ggtcattttt gaagatttca cgtgcctcgt ctttagtcac ttcttcacga
    44821 atagatggga agttttcttt gacgattttt tgcatttctt cttcgatacg aggaaggtct
    44881 tcgttagaga tttgaccagc tgtgttgtca gtatcgtagt agaaaccatc ttcgatggct
    44941 ggaccaactc ccaagtgaat gtctgggaaa agacgacgag ctgcttgggc gaacaagtga
    45001 gctgctgagt gacgcaagat tggaagggca tcttcgtgat caggtgtcac aatttcgatg
    45061 cttccatctt cagtgatagc gcgagtagtg tcgatgagtt tgccgttgaa tttaccagcc
    45121 aaggcttttt tagctaggga attgctgata gattgggcaa tttcaaaagt tgttacgcca
    45181 gattcgaatt cacgaacagc gccatctggg aaagtaatgt taatcatgat gttctccttt
    45241 tttattattt catagtttct aagcattgat tttttatcag cagactactt ccaagtccta
    45301 acgttcaatt gcttttcttt atcagagagg aagttttcca tttccgtcag aatgtctata
    45361 tccaaacgtt gagaaagttc taatagccac cagatatttt ctgaaagttt ttgttccagt
    45421 gtgtagggtg tttcatcata gtagcgtcct tgctttgtca tcaccagtcg ttggaaattt
    45481 ccaatatcat tagataaagc caagaggtct tcttctaccg tccacttgga atcatgatgc
    45541 ttaacttcca gttcgtgata agcttgtcgg attgcccaac tgcgctccac taattcttgc
    45601 aactccataa ttagtatcct aataaaaaaa tgcgacatcc tcgaaaggac gtcgcaacgt
    45661 ggttccacct tcatttatgt acctcaaaag agagggacac ctctgattgg ctctaacgtg
    45721 gccaccgttt tatgttttca taaaaaactc aagagtagta tcagtttaga cttcctatgc
    45781 gtttccagca accacgcact ctctagtaga aagggactaa gtgacttgtc tctatggatt
    45841 attatagcat gattgtgaga tttttcaagg attttgtgaa agttttttgt ttttcatatt
    45901 ttgtagaata tgcttgatgc taataccaga atccatgccc accagagcaa ctgtttcata
    45961 gattagcaag taaattagta taaactcacc aaaggttatg atagtaaagt aaaagagcag
    46021 tccgttacca actaaccatc aaagtgagaa acggaaacgg tagctataga gtaacgatac
    46081 aatcaagatt accagaggtg taaagaagag aagattgttt atatagtgta agggggtcag
    46141 aaacaactag caagaacaac tcataaagtg gccaataaaa gaataatatg accagatagt
    46201 aggggagatg ggaatagaat ctacgcatga taatcacctc gttttaaaaa aacagacaac
    46261 ttagatctct tgctatgatt attatagcat gtttaaaaca agaaagtaaa tttgttgaca
    46321 aagaagattt ttcttttcac tagaatcatg gttagttttc ttggtagaaa tattttgata
    46381 ataattaagc aagactattg acattgatta ttaagagtgt cacaattgtt gtaactaaaa
    46441 caattacaac aagagaggta taaaaaatga aactagcagt tattgcagca aatggacaag
    46501 cggggaaggc aattgttgaa gaagcagtca aacgaggaca tgaagtaacg gctattgttc
    46561 gttctgaaaa caaaagtcaa gctgagtcta ttattaaaaa agatttattt gagttgacaa
    46621 aagatgatct tactggattt gatgctgtta tttcagcgtt tggtgcttat acaccagata
    46681 cgctaccgct ccacagcaaa tcaatcgaac tatttaatca gcttcttgct ggaacacaga
    46741 cacgttttct agttgtaggg ggatcaggaa gcttttatat agatgaaaca aaaacgacac
    46801 gacttttaga tacgtctgat tttcctgaag aatttaagcc actagcaaaa gctcaagcgg
    46861 atgaattgga tcttcttcgt acaaaaaata atctcaactg gacttttgtc agtcctgctg
    46921 tagattttat tcccgatggt gaaaaaacag gaaactacat tctagcagga gaaattttta
    46981 ctactaatga aaaaggaata agccagatta gttatgcaga ttatgctatt ggattggtag
    47041 atgaacttga aaaaggtcat catataaaag aacgtatctc tttacttgaa aaataataac
    47101 tatgagagta ttaatgtttg acagaaaatt taaatcttgg taaaataggg attgtcgtaa
    47161 agacaaataa cttcttcttg gttacaggca tgccaacctg tcactcggat gaagccaaat
    47221 aaaaaggaga aacatcatgg caatctcaaa agagaaaaaa aatgaaatca tcgcacaata
    47281 tgcacgtcac gaaggtgata caggttcagt agaggttcaa gttgctgtcc ttacttggga
    47341 aatcaaccac cttaatgaac acatcaaaca acacaaaaaa gaccacgcta cttaccgtgg
    47401 attgatgaaa aaaatcggtc gccgtcgtaa cttgcttgca tacttgcgta aaaacgacgt
    47461 taaccgttac cgtgagttga tcaactctct aggacttcgt cgctaattca agatacaaag
    47521 gctgtcaaaa gcacaaagca aaaataggaa aattgacgaa gaaacttcag tttctaggag
    47581 attttatctt ttttgccaag tgcttaggcc gtgttcaatt gagcatatct tgataatgaa
    47641 gctactctaa attgggtagc ttttttgcgt tgtcacctta cctcgatata ctcaagtata
    47701 atcttcggtt acggttccta gcactgtaag gtaaaataaa ccagcttttc tcccttcggg
    47761 gagatttttt tttgtttcac tgaaagtttc gtacgatctt cgcctcgtcg cctagtctat
    47821 aaacgattta tccagcaaga atcatgatgc taagggcgtc aaaaatccgt atgaaaatag
    47881 ggaaatggca cagtgttcga tgaacgcaag gagtttcatc tttttcacta ggattttagt
    47941 ccgggctcaa atcagctctc tgacttcaga gggctttttt attgaggttt tatcggtaac
    48001 aattttggag actacaaaaa cctcaaatgg tatcagctaa ttacaccata aaggtgcgca
    48061 gttactcggc ttttcaagcc gagtagctgt atgcaagccc cctagaagag cccactcttt
    48121 acaaagtaaa gtatagtatg ttatacttta catggaagta gtcactgaat tccagttaga
    48181 aattactttg taactacgtt ttgaggagga gtaaaatgct ttcctacgtt cgacattacc
    48241 cactagcgat agctaaatta atgtgtctgt gctctcctaa aatctgctga tttattactg
    48301 actaatacag gaggtttttt tatgggacag acaatcatat ctgctattgg tgtttatatt
    48361 tccaccagta tcgattattt aattatttta attattttat ttgcacaact atcacagaat
    48421 aaacagaaat ggcatattta tgcggggcaa tatctaggca caggcttact tgtaggggcg
    48481 agtttagttg ctgcttatgt cgttaatttc gtgcctgaag aatggatggt tggattgctt
    48541 ggtttaatcc ctatctattt agggattcgc tttgcaattc ttggagaaga tgcggaagaa
    48601 gaagaggaag aaattattga aagattggaa caaagcaagg caaatcaact gttttggaca
    48661 gttacattgc tgacaattgc gtctggcgga gataatttag gtatctatat accttatttt
    48721 gcttcgttag attggtcaca gaccctcgtg gccttgcttg tgtttgtaat cggcataatt
    48781 atcttttgcg agattagtcg ggtgttatcc tctattccgt taatattcga gacaattgaa
    48841 aaatacgagc gaatcattgt gcccttagta ttcattctac ttggactata catcatgtat
    48901 gaaaatggca cgatagagac ttttctgatc gtgtagattt ttttgtttca ctaggatttt
    48961 agcccgagct caaatcagct ctctgatttt cagagggctt tttcattgag tataaatgga
    49021 caatcttaaa gttactctat tgtagagtgg cttttgattt gattaggaga ttcttataaa
    49081 tatagtaaaa tgaaacaaaa acaggacaaa tcgatcgtga cagtcaaatc gatttctaaa
    49141 aatgttttaa gaagtagagg tgtactattc tagtttcaat ttgatatata aatgaaaacc
    49201 ttagctgttt ggccaaggtt catttcttct atgcaagttc ttctcttttc ttatctgggt
    49261 tccagatgaa gcttgcgata aagctaaaga tgatggttag gatagcgagg ataatagcaa
    49321 ggatgagggc agggatgcgg ataaaccacc agagtggatt tggtttttta tcattgtgcc
    49381 attgtttcgt ttcttcgtcc agacgttgga attctgtttg aatacgattg gcgactgttt
    49441 caatgccttc atcattcatt ttcttgatat ctgagatatc gattggattt ccaaagttca
    49501 tatcgacacg ttcacggcta atcaagccct tcaaagtcat gggaccggtg taggtaaccg
    49561 gcatgatacg gaccttggcc attttggcaa tcagtgctgc gccccccttg acatcgtttg
    49621 agtggcggct accacttgga aacatgatga gagagcggtc actttttttg agaacgttga
    49681 taggatattt gatggctgag gcgctgggat tttcacggtc gatgggaaag gcgccacaca
    49741 tacgaatcca ccaaccaaag atacggttgg taaagagttc tttttttgcc ataaagatga
    49801 actgttttgg cttggtcgca aaggccatat aaacaggatc ccaccaggta cggtgaggcg
    49861 caactaaaat ataattttca tcttgattag gaattttatc agtattatga tagtgagcat
    49921 tgccattgat ggaccataag agcaatacaa ctaatccacg caaataagta taaaacatgc
    49981 gatctccttc gattgttttc ttgttattat tataccttat caaaggaggg ctggcaaact
    50041 tttcccttga ctagatgcat atttgggatg aaattagaat tctgttaaaa aaaatgatat
    50101 aatagaattt atggataaaa ataagattat gggattaacc caaagagaag tcaaggaaag
    50161 acaggctgag ggtttggtca atgactttac cgcatcagcc agtaccagca cttggcaaat
    50221 cgttaaacga aatgtcttta ccctttttaa cgctttgaac tttgccattg ctttggccct
    50281 tgcctttgtg caggcttgga gcaatctggt cttctttgct gttatctgct ttaacgcttt
    50341 ttctgggatt gtgaccgagc tacgagccaa acacatggtg gacaagctca atctcatgac
    50401 caaggaaaag gtcaaaacca tccgtgatgg tcaggaagtt gctcttaatc ctgaagaatt
    50461 agtgctagga gatgtcattc gtttgtctgc aggagagcag attcctagtg atgccttggt
    50521 tttggaaggc tttgcggaag tcaatgaagc catgttaacg ggagaaagtg atttggtgca
    50581 aaaggaagtt gacggcttac ttttgtcagg aagtttccta gccagtgggt cagttttatc
    50641 tcaagttcac catgtcggtg cagacaacta tgctgccaaa ctcatgcttg aggctaagac
    50701 cgttaaaccc atcaactccc gtatcatgaa atcgctggac aagttggctg gttttactgg
    50761 gaagattatc attccctttg gtctggctct cttgctggaa gccttgcttt taaaaggcct
    50821 gcctctcaag tcatccgttg taaactcgtc gacagctctt ttgggaatgt tgcctaaggg
    50881 aattgccctt ttgaccatta cttcgctctt gactgcagtg attaagttgg gcttgaaaaa
    50941 ggtcttggtg caggagatgt actctgttga gaccttggcg cgcgtggata tgctctgtct
    51001 ggacaagacg ggcaccatca cccaaggaaa gatgcaggtg gaggctgttc ttccactgac
    51061 ggaaacttac ggtgaagagg ctattgccag catcttgact agctacatgg cccatagtga
    51121 ggataagaat ccaactgccc aagccattcg ccagcgtttt gtgggagatg ttgcttatcc
    51181 tatgatttcc aatcttccct tctcgagcga ccgcaagtgg ggggctatgg agttagaagg
    51241 cttggggaca gttttcttag gggcacctga gatgttgctg gattctgaag tcccagaagc
    51301 cagggaggcc ttggagagag gatcacgtgt cttggtctta gctctcagtc aggagaaatt
    51361 agaccatcac aaaccacaga aaccatctga tattcaggct ctagccttgc tggaaatctt
    51421 ggatcctatt cgagagggag cggcagagac gctgaactat ctccgttctc aggaagtggg
    51481 gctcaagatt atctctggtg acaatccagt tacggtatcc agcattgccc agaaggctgg
    51541 ttttgcggac tatcacagct atgtcgattg ttcaaaaatc acggatgagg aattgattgc
    51601 tctggcggag gagacagcga ttttcggacg tgtttcccct catcaaaaga aactcattat
    51661 ccaaacgctg aaaaaagcgg gtcatacaac agctatgact ggggatggag ttaatgatat
    51721 cctggctctt cgtgaggcgg attgttctat cgtaatggcg gagggagacc cggcaacccg
    51781 tcagattgcc aacctggttc tcttgaactc agactttaat gatgttcctg agattctttt
    51841 tgaaggtcgt cgtgtggtca ataacattgc ccacatcgct ccgattttct tgataaagac
    51901 catctattcc ttcctgttag cagtcatctg tattgccagt gctttactag gtcggtcaga
    51961 gtggattttg attttcccct tcattccgat ccagattacc atgattgacc agtttgtgga
    52021 aggtttccca ccatttgttc tgacttttga gcgaaatatc aaacctgttg agccaaattt
    52081 cctcagaaaa tccatgcttc gtgccctacc aagtgccctc atggtcgtgt ttagcgttct
    52141 ttttgtgaaa atctttggaa cgagtcaagg ttggtctgag ttagaaatct caactctcct
    52201 ctattatctc ttggggtcaa ttggtttctt atccgtattt agagcctgca tgccattcac
    52261 tctatggcga gtcctcttga ttgtctggtc agtaggaggc ttcctagcca cagctctctt
    52321 cccaagaatc caaaaactgc ttgaaatttc aaccttgaca gaacaaacgt tgcctgttta
    52381 tggtgtcatg atgttggtct ttacagtgat tttcatcctg actagtcgct atcaagctag
    52441 aaaataaaga aagactgcaa tctgtggatt gcagtttttt atacgatgga tctatcgtag
    52501 atctgatgtg caaggcctac gtgtctatta tctatcggtg aacccaagag tgattctcaa
    52561 gcctgcttgg attgaggtaa tagattcaaa tatctgtagt tagactattt gaagtttgat
    52621 gtaagaaaga gaaagcgaca gattgaagta attttaactc tcttctattg ctagaacaaa
    52681 tggtcggata ggttggtagt ttgaaaatga agatgcaatc tattgttaaa tggaacatag
    52741 tgttatttat tagaaaatcg tttggtttat ttcttatcaa atacgaaaag caacttaaat
    52801 atttcaacta aaatagatgt tatgaagaaa aggtaaaatg attttggtat agtgaggttc
    52861 tgttctattt gatatcatat ttttgataaa aacaaaaatg tccattgcaa aggacaaaat
    52921 gcgaagtata ttattttttg aaagcgatat aatggaatca taaaggaggt gtatcgtgtc
    52981 tagaaaacaa gaacaaatgg aaacgttgtt gctccttttg cgagatagta aggattatat
    53041 atctgctaaa gtattgggag aaaaattaaa ttgctctgat aaaacggttt atcgccttgt
    53101 caaggtaatc aacaaagatt gtccggtaga agcattcatt ttatctgaaa aaggcagagg
    53161 tttcaaatta aatccaagaa gttccctcgt ggacgttgat gggaatttta cagaggcttt
    53221 tgatcctgaa gtaaggcgtg aaaaattact agaacgtctc ttgttgactg ctcctaagcc
    53281 acattctatt tatgatttag gagaggaatt ctacgtaagc gagtcagtag tactaaaaga
    53341 tcgtcagata ttacaagaga gtctagcaat ttatgggtta gatttaaaaa tgagacaacg
    53401 aaagcttttt attgatgggg atgaggctca aattcgttca gccattctaa atctactgcc
    53461 aatgtttaat cagttggatt tagagcaaat tacacagaat aaggttcagc ctcttgacgg
    53521 agaacttgct cacttttgtt tgggattact gattacactt gagagagaat tgggggtaaa
    53581 cattccctat ccatataata taaatatttt ctctcacctg tatattttta tcagtaggaa
    53641 tcgtcgtagt actagtattc atgttgtagc accttcaaaa cctactattg ttgatgagaa
    53701 aatttacagt gtctgtcaaa aaattattca agaaattgaa caatatttta agatgaaggt
    53761 tgatgcagtt gagattgact atctttatca atacgttgta tcttcgagat tgcaaaaacc
    53821 attttcttcc gggaagcttc ctttttctca gcgagtttta gatgtcactc attactattt
    53881 tagccgtatg tgtatggaca atagagagat tgaaatgaca gatcctgact ttgttgactt
    53941 ggcgagtcat atcagtccct tactgaggag attagataat agagtacaga ttaagaatag
    54001 tcttttatca caaattcttt taacctatcc taatctagtt aaagagttaa caactatttc
    54061 taaagaagtg agtctagtat ttggttttgc gtccttgagt ctggacgaga ttggttttct
    54121 agtcttatat tttgcacggt ttcaagaaaa gcgagcacgt cctctaaaaa cagtagtgat
    54181 gtgtacatca ggtgtcggaa cttcagagct tttacgagca cgattagaaa agcaatttac
    54241 tgaattggat attattgatg tagttgctta tcatcaatta gatgagctga taaatctata
    54301 tccagattta gatttcattg tgacgacggt agctttgcag gaaccagcaa gtgtcccgtt
    54361 tgtcctagtt agtgtttttc taaccgaggg tgataaacaa cgtcttcaag caaaaattca
    54421 ggagataaac tatgaataat ctttcgcttg tccttatgga tatatctgtt caaaatcgtc
    54481 aagaagccta caaagaatta gcaaatcaaa tcagccttct tgtttctgaa gatacagaaa
    54541 aaatagaaga gcttctatat taccgtgaga gacagggaag tatagaggtt gctaaaggtg
    54601 ttcttctacc acattgtgaa ggaaactttc aacatcatgt cttagtgatt actagattaa
    54661 aatcacctat cagagaatgg tcgaaggata tccagtgtgt tgaccttatt atcggtttgg
    54721 ccattgcagt atcacaggac aagtcatgta ttaaaacatt gatgagaaga ctagcagatg
    54781 aatcattcat aaatcaatta aaacagttaa caaaagaaga attacgggag ataatatatg
    54841 gaaatcaaag atattcttaa tgtgagtctg atccagacgg atttacagat gcagagcaaa
    54901 gaagaggttt ttgaggcatt agctcaacta ttggttgaga cgggttatgt gtctgataga
    54961 gaccaattta tcgaaggtct gtatcagaga gaggcagaag gacagaccgg tattgggaat
    55021 tatattgcta ttccccatag caagagttct gctgtggaga aggcgggggt agtcatagct
    55081 ataaatcaca atgagattcc ttgggagacc attgatggga aaggggtcaa agtaattgta
    55141 ctctttgcag ttggtgatga tacagaagct gctagggagc atttgaagac cttatcactc
    55201 tttgctcgaa aacttggtaa tgacgaagtt gttgccaaat tagttcgggc tcagacatct
    55261 gatgatgtga ttgcagcttt ttgttaataa gaaaaaattt tggagggtat ccgtatgaaa
    55321 attgttggtg ttgcagcttg tactgtggga attgcccaca cttatattgc acaggaaaaa
    55381 ttagagaatg ccgcaaaggt agctggacat gtgattcatg ttgagactca ggggacaata
    55441 ggggtagaaa atgaattgag tcaagagcag attgatgcag cggatgtagt tattttagca
    55501 gttgatgtta agatttctgg tatggaacgc tttgagggta aaaagattat caaggttcca
    55561 acagaagtgg cagtcaaatc tcccaataaa ctgattgcta aagctgttga gattgttacg
    55621 aaataactga aaatatttaa ggagaaaata tatgttgaaa cacttaaact taaaaggtca
    55681 cttattgaca gccatttcct atatgattcc aattgtttgt ggtgcaggat tcttagttgc
    55741 cattggttta gcaatggggg gtggtgttcc tgacgctctt gtagcaggaa aattcactat
    55801 ctgggatgct ttagcaacta tgggtggtaa agcccttggt ctcttgccag ttgttattgc
    55861 tacaggtttg tcttactcga ttgctggtaa gccagggatt gcaccaggtt ttgttgttgg
    55921 tctaattgcc aattctgttg gttcagggtt tatcggtggt atcttgggag gttatatagc
    55981 tggtttcttg gttcaagcga ttattaaaaa ggtcaaagta ccaaactgga ttaaaggttt
    56041 aatgccaacc ttgattattc cttttgtagc ctctttggta agtagtttga ttatgattta
    56101 tattattgga gcgcctatcg cagcctttac caactggttg acgagcttat tacaaagctt
    56161 gggaagtgct tcaaatggtt tgatgggggc agttattgga gttctcagtg ctgttgactt
    56221 tggtggccca cttaataaaa cagtctatgc gtttgtgttg actttacagg ctgaaggtgt
    56281 gaaagaacca ttgactgctt tgcaattggt gaatactgct acaccagttg gatttggatt
    56341 ggcctatttt atcgcgaaat tactcaaaaa aaatatctat actcaagagg aaatcgaaac
    56401 attgaaatcg gctgttccta tggggattgt caatattgtt gaaggtgtaa ttccgattgt
    56461 tatgaataac ttggttccag gtctcattgc aacaggtatc ggtggtgctg ttggtggtgc
    56521 tgtttctttg acaatgggtg ctgattctgc cgtgccattt ggtggagtgc ttatgttacc
    56581 aaccatgact cgtccagtag ctggtatttg tgctttgtta gccaacattg tagtcacagg
    56641 acttgtctac gcgattttga aaaaaccaat aaaacataca gaaccagtta tgactgttga
    56701 agaagagatt gatttgtcag atattgaaat tttgtaagag ggtaacgatg tcaagaattg
    56761 aattttcacc atctttgatg accatggatt tggacaaatt caaagagcag attacttttt
    56821 tgaatgataa agtagcatct tatcatatcg atattatgga tggccatttt gttcccaata
    56881 ttaccttgtc tccttggttc attcaagaag ttcaaaaaat tagtgacaca cctttatcag
    56941 ttcatctgat ggtcacagac ccaacctttt gggtagatca agttctcgat ttacaatgtg
    57001 agtatatttg tattcatgct gaagttctga atggtcttgc ttttcgtttg attgataaaa
    57061 ttcatgatgc aggtctaaag gctggtgttg tccttaatcc tgaaacacct gtttctacaa
    57121 tctttcccta cattgattta cttgacaaag taactattat gactgtagat ccaggttttg
    57181 caggacaacg ttttttgaag tctaccttgt ataaaatcca agaactccgt cagcttagag
    57241 ttcagaatgg ttatcactac atcattgaga tggatggttc ttcgagtcgt aagactttca
    57301 aacaaattga tgtggcagga ccagatattt atgttatagg tcgcagtgga ttatttggtt
    57361 tggatgacga tattgccaaa gcctgggata tctgttctag agattacgaa gaaatgaccg
    57421 gaaaaacaat gccaatcaaa taatggtttg agaagaaatt tattagttag gaggaatata
    57481 tgtcactaca atcagttaac gccattcgtt ttcttggcgt agatgctatt aacaaatcta
    57541 attctggtca cccgggaatc gtcatgggtg ctgcgccaat ggcttatagc ttatttacaa
    57601 aacaccttag aattacacct gagcagccaa actggattaa ccgagatcgc tttatcttgt
    57661 ctgcgggtca tggatcaatg ctactgtatg ctctcttgca tttaacaggg tataaggatg
    57721 tatccatgga cgagattaaa aatttccggc aatggggatc taagacacct ggtcatcctg
    57781 aagtgacgca tacgtctggt gtggatgcga catctggtcc gcttggtcag gggatttcta
    57841 ctgccgttgg tttcgcccaa gcagagcgtt ttttagctgc taagtacaac aaagatggtt
    57901 ttcctatttt tgaccattat acttatgtta tcgctggaga cggtgacttc atggaaggag
    57961 tgtctgcgga ggcggcttct tatgcaggtc atcaagcttt agataagctt atcgtcctct
    58021 acgactccaa cgatatctgc ttggatggtg agaccaaaga tactttctct gaaaatgttc
    58081 gcgcccgtta cgatgcttat ggttggcata cagttctggt agaagatgga acagatttag
    58141 cagcaatttc tacagcaatt gagacggcca agttttctgg taagccgagt ttgattgaag
    58201 tgaaaacggt aattggttac ggctcaccca ataaaagtgg tacaaatgct gttcatggtg
    58261 caccactagg agcagaagaa acaggagcaa ctcgtaagtt tttgggatgg gattacgatc
    58321 catttgaagt accagaggaa gtatattctg atttcaagac aaatgtagcg gatcgtggtc
    58381 aggaggcata cgatgcttgg gctagtttgg tgtctgatta caaggttgct tatcccgaag
    58441 ttgctagtga gattgacgct attgtagctg gaaaatcccc tgtaaccatt actgaaaaag
    58501 acttccctgt ctatgagaat ggcttctctc aagcaactcg taattcgtcc caagatgcta
    58561 ttaatacagc agcagcagtt ttaccaacct tcttaggtgg atcggcagac ttagctcact
    58621 ccaacatgac ctacatcaag gcagatggct tacaagataa atataatcca ttaaaccgca
    58681 atattcagtt tggggtacgt gaatttgcca tgggaacaat cctcaatgga atggctcttc
    58741 atggtggttt acgagtttat ggcggaacct tctttgtttt ctctgactac gtcaaagctg
    58801 ctattcggct atcagccatt caggagttgc ctgtaactta tgtctttacc catgattcaa
    58861 ttgccgttgg tgaagatggt ccaactcatg aaccagttga acatttggca ggtttacgct
    58921 caatgccaaa cttgactgtt atccgtccag cggatgcccg tgaaactcaa gcggcttggc
    58981 atcatgcctt gaccagtacc accactccaa ctgtcattgt cttaacccgt caaaacttgg
    59041 tagttgaaga agggacagac tttggtaagg tcgctaaagg agcctacgtc gtgtatgata
    59101 ccccgggatt tgatactatt atcattgcta caggatctga ggtcaatcta gctatcaaag
    59161 ctgctaagga attggtttta caaggtggta aagtacgtgt ggtatctatg ccctcaaccg
    59221 aactatttga tgctcaagat gctacctaca aggaagacat tttaccatct aagactcgtc
    59281 gtcgtgtggc cattgaaatg gcagcgaccc aaagttggta caagtatgtt ggtttggatg
    59341 gcgcggtcat cggtattgac atcttcggtg cgtctgcccc agctcagact gtgattgata
    59401 attatggatt tacggtagag aatatcgttg ctcaagttaa gtccctatag aaaccaatta
    59461 caatgaagat acagctgttg tcagactagc agatgtagtg atagacacta atcagatgat
    59521 tggttattta aaaactataa tgagaatgta ataatttatc tacgaaagtt atagtagata
    59581 gtatacacaa tagagtatac cctgaaacgg ttgcgaagta cgctaatcac tttgctactg
    59641 atctagatag tttctttaat caataaacac agcatccaca gattgactta ggatattgta
    59701 agttttttga aagctagaga gaaggtctct aaaattaaaa aacgcatagt ataggatgtt
    59761 gaaatgatga actgcccccc aaaagttaga cagaaaaaat ctaacttttg gggtgtttta
    59821 ttatgaaatt aagttatgaa gataaagttc agatctatga actaagaaag caaggacaaa
    59881 gcttcaaaca gctttcaaaa agatttggtg tggatgtttc tggtctaaag tacatggtga
    59941 aattaattga ccgttatgg
//
LOCUS       DAVBDG010000005        55657 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBDG010000005 DAVBDG010000000
VERSION     DAVBDG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.005"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            3..308
                     /locus_tag="VQ526_000270"
     CDS             3..308
                     /locus_tag="VQ526_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2554047.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            356..1831
                     /locus_tag="VQ526_000271"
     CDS             356..1831
                     /locus_tag="VQ526_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2554048.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAVIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2091..8792
                     /locus_tag="VQ526_000272"
     CDS             2091..8792
                     /locus_tag="VQ526_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2554049.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKLEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIANTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLVTVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLVTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIHP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVDLTIQANTEKKIAQSIRP
                     VNVVTDLHQEPTLPSTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARARVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEITVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKDIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSASSQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9040..9896)
                     /locus_tag="VQ526_000273"
                     /pseudo
     CDS             complement(9040..9896)
                     /locus_tag="VQ526_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10107..10466)
                     /locus_tag="VQ526_000274"
     CDS             complement(10107..10466)
                     /locus_tag="VQ526_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2554050.1"
                     /translation="MGMILMKLASILLLILTLVICIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLTLSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYVEMIVELFLMK"
     gene            10558..11514
                     /locus_tag="VQ526_000275"
                     /pseudo
     CDS             10558..11514
                     /locus_tag="VQ526_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11498..12055
                     /locus_tag="VQ526_000276"
     CDS             11498..12055
                     /locus_tag="VQ526_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2554051.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12048..12302
                     /locus_tag="VQ526_000277"
     CDS             12048..12302
                     /locus_tag="VQ526_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554052.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12314..14368
                     /locus_tag="VQ526_000278"
     CDS             12314..14368
                     /locus_tag="VQ526_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2554053.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEKSKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14547..15407
                     /locus_tag="VQ526_000279"
     CDS             14547..15407
                     /locus_tag="VQ526_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2554054.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            15626..16336
                     /gene="ccdA2"
                     /locus_tag="VQ526_000280"
     CDS             15626..16336
                     /gene="ccdA2"
                     /locus_tag="VQ526_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2554055.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16356..16922
                     /locus_tag="VQ526_000281"
     CDS             16356..16922
                     /locus_tag="VQ526_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2554056.1"
                     /translation="MKKWQTCVLGAGSLLWLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            16933..18045
                     /gene="msrB"
                     /locus_tag="VQ526_000282"
     CDS             16933..18045
                     /gene="msrB"
                     /locus_tag="VQ526_000282"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2554057.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18358..19095
                     /locus_tag="VQ526_000283"
     CDS             18358..19095
                     /locus_tag="VQ526_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2554058.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19092..20783
                     /locus_tag="VQ526_000284"
     CDS             19092..20783
                     /locus_tag="VQ526_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2554059.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            20899..21462
                     /locus_tag="VQ526_000285"
     CDS             20899..21462
                     /locus_tag="VQ526_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2554060.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            21484..27126
                     /locus_tag="VQ526_000286"
     CDS             21484..27126
                     /locus_tag="VQ526_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2554061.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQFPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQQENPKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETKTAKGTQEKGHVGEALAQEQLPEYKVTEGTLVETSTTDLDYKTET
                     TEDPTKYTDEETVLRTGEKGSQVTKTTYKTVEGVKTDQVLSTSTEVTKEPVNQQVIRG
                     TKPIEGTLIEESLEKIPFKEVVKEDDQLKKGLEVVAQEGKEGQKKITKTFNTIKGVKT
                     EDAPKVTEEILEEPQDKILKRGTKSFEKPVLTITEIESKDLKRTSDVKYSLENPSKAA
                     IKSITLTLKKGDEIVKTLNVSPEDLTTTLTDLQYYKDYKLETKMVYDRGEGDEEEVLK
                     EEPLRIDLKKVEIKDIIRTDLVKYENQVETDETRLTSVPTDLSNYYLKVTSNDHKTNL
                     LAVQKIEESTIDGKQVYKVIAVADNLVQRNVDNRFEETYTYYIEKPKASQAGIFYDFE
                     ELIQAIQTNPAGEFKLGQSMSARNVVPKGKSYITSEFTGKLLSDGDKRYAIHNLEHPL
                     FDKIKAGTVKNINFENIDIDLSNQDNIAAIANEISGTTRIENIKAIGTITARNNVAGF
                     VNNIKEDATLENVAFIGSINSAGNNSTVGGIAGSNYMGFVNRAYVDATITANNANASM
                     LVPFVQYKAHSWLSGTKGKLTNSVAKGILDVKNTKYVGGITSKTWPYGAVQDNVTYAK
                     VIKGQEIFGSNDVNDGDANPFVSNLFGVIGYSSSEDGTGKDNKNKNKLKHLSKSEADA
                     KVASFNITAAKFVSEPYELNKLNNVSSREAAYESIQDYNADYKLAYKNIEKLQPFYNK
                     DFIVNQANKLDKNNKLNTKEILSVTPMNNNNFVTDLGDANKIIVHYADGTKDYFNLSS
                     SDEGLSNVREYTITDLGIKYTPNIVQKDHSALINGIVNILSPIELQSNPIYQKLGRTG
                     SNRVNAIKDLYLEESFDHVKANLNILVTKLVENEDHQLNNSSAAQQMILDKVEKNKAA
                     LLLGLTYLNRYYGVKFGDVNIKELMLFKPDFYGKNVSVLDRLIEIGSREDNIKGHRTY
                     DAFSEIISKTTLSENLVNFLNYNRRLFTADVNIYDWFKKATEGNVYIAERASEVDEIK
                     AAKYKVWDNLQSATHRKMILPLLNLNKSHVYLISNYSAMAVGTAEKLGHSSFDGIKNQ
                     IEKAADSYRNYFDFWYRLSLDKVKNMLVKNTVTPLWEGYQAPGGWPDKYGKRNNKEDY
                     APLRELFGPIGKYYGNNGTGAYAVIWDNPLDTRTEVNYIALSMIDEFGISVYTHETTH
                     VNDRAIYLGGYGRRSGTHAEAYAQGMLQTPVPSTWFDEYGALGINMTFYRPNDGNQWY
                     ITDPKTLKTREDIDNYMKGYIDTLSLLDYVEGDAVISKHDDKLNQLWFKKVDPIERTV
                     GSKDQYDKVRNMSSDDWKGITLNSVNDLVDNNLMTNRMMNNNIYKPEVFDPGIAYVGI
                     KMMNSIYAGNTSEGAPGAVTFKNNAFRLWGYYGYDKGFLGYASDKLKSQANKEGKQFG
                     DQYIIQKISDGRFQSFADFKKEYYKELLEKANAGFTPFTIDGETIHNYDELKSLFAKA
                     LEEDMVTNKVNGKLVQVKEKVYKKLLQQTNSFKTSIFK"
     gene            27187..28908
                     /gene="pabB"
                     /locus_tag="VQ526_000287"
     CDS             27187..28908
                     /gene="pabB"
                     /locus_tag="VQ526_000287"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2554062.1"
                     /translation="MHRKTVIDFRILGERYTFTQPIKEIKTRNVAEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGKLLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            28909..29526
                     /locus_tag="VQ526_000288"
     CDS             28909..29526
                     /locus_tag="VQ526_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2554063.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            29576..30573
                     /locus_tag="VQ526_000289"
                     /pseudo
     CDS             29576..30573
                     /locus_tag="VQ526_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            30700..31659
                     /locus_tag="VQ526_000290"
     CDS             30700..31659
                     /locus_tag="VQ526_000290"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2554064.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            31756..32595
                     /locus_tag="VQ526_000291"
     CDS             31756..32595
                     /locus_tag="VQ526_000291"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2554065.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(32643..32813)
                     /locus_tag="VQ526_000292"
     CDS             complement(32643..32813)
                     /locus_tag="VQ526_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2554066.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            32934..33818
                     /gene="miaA"
                     /locus_tag="VQ526_000293"
     CDS             32934..33818
                     /gene="miaA"
                     /locus_tag="VQ526_000293"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2554067.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            33811..35049
                     /gene="hflX"
                     /locus_tag="VQ526_000294"
     CDS             33811..35049
                     /gene="hflX"
                     /locus_tag="VQ526_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2554068.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            35042..35665
                     /locus_tag="VQ526_000295"
     CDS             35042..35665
                     /locus_tag="VQ526_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2554069.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            35680..36609
                     /locus_tag="VQ526_000296"
     CDS             35680..36609
                     /locus_tag="VQ526_000296"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2554070.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            36630..37385
                     /locus_tag="VQ526_000297"
     CDS             36630..37385
                     /locus_tag="VQ526_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2554071.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(37439..38347)
                     /locus_tag="VQ526_000298"
     CDS             complement(37439..38347)
                     /locus_tag="VQ526_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2554072.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            38411..38680
                     /locus_tag="VQ526_000299"
     CDS             38411..38680
                     /locus_tag="VQ526_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2554073.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            38680..39060
                     /locus_tag="VQ526_000300"
     CDS             38680..39060
                     /locus_tag="VQ526_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2554074.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            39149..39376
                     /locus_tag="VQ526_000301"
     CDS             39149..39376
                     /locus_tag="VQ526_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554075.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(39416..40141)
                     /locus_tag="VQ526_000302"
     CDS             complement(39416..40141)
                     /locus_tag="VQ526_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2554076.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTNLKRIQFGDFTLNPDLA
                     EGNYHPLNQKELQIIKNYLEMSR"
     gene            40303..42144
                     /gene="typA"
                     /locus_tag="VQ526_000303"
     CDS             40303..42144
                     /gene="typA"
                     /locus_tag="VQ526_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2554077.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            42164..42418
                     /locus_tag="VQ526_000304"
     CDS             42164..42418
                     /locus_tag="VQ526_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2554078.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            42784..43083
                     /locus_tag="VQ526_000305"
                     /pseudo
     CDS             42784..43083
                     /locus_tag="VQ526_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            43154..45175
                     /locus_tag="VQ526_000306"
     CDS             43154..45175
                     /locus_tag="VQ526_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2554079.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            45177..45818
                     /locus_tag="VQ526_000307"
     CDS             45177..45818
                     /locus_tag="VQ526_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554080.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            45922..47274
                     /locus_tag="VQ526_000308"
     CDS             45922..47274
                     /locus_tag="VQ526_000308"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2554081.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            47276..48334
                     /locus_tag="VQ526_000309"
     CDS             47276..48334
                     /locus_tag="VQ526_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2554082.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            48344..49543
                     /gene="divIB"
                     /locus_tag="VQ526_000310"
     CDS             48344..49543
                     /gene="divIB"
                     /locus_tag="VQ526_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2554083.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            49776..50477
                     /gene="pyrF"
                     /locus_tag="VQ526_000311"
     CDS             49776..50477
                     /gene="pyrF"
                     /locus_tag="VQ526_000311"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2554084.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            50511..51143
                     /locus_tag="VQ526_000312"
     CDS             50511..51143
                     /locus_tag="VQ526_000312"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2554085.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            51323..51726
                     /locus_tag="VQ526_000313"
                     /pseudo
     CDS             51323..51726
                     /locus_tag="VQ526_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51822..52586
                     /locus_tag="VQ526_000314"
     CDS             51822..52586
                     /locus_tag="VQ526_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554086.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            52586..53338
                     /locus_tag="VQ526_000315"
     CDS             52586..53338
                     /locus_tag="VQ526_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554087.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            53348..54046
                     /locus_tag="VQ526_000316"
     CDS             53348..54046
                     /locus_tag="VQ526_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554088.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            54043..54690
                     /locus_tag="VQ526_000317"
     CDS             54043..54690
                     /locus_tag="VQ526_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554089.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(54777..55643)
                     /locus_tag="VQ526_000318"
     CDS             complement(54777..55643)
                     /locus_tag="VQ526_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2554090.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHLTYGDAAK
                     ADDLVVEGGKVD"
BASE COUNT        16980 a        10115 c        12225 g        16337 t
ORIGIN      
        1 aaatgattaa aattcttgct gcctgcggtg caggtgttaa ctcaagtcac caaattaaaa
       61 gtgctctaga agaagaactt tcaaaccgtg gttatgatgt tcactgtgat gcagtcatgg
      121 tgaaagatgt aaacgaagac cttatgaaag gttacgatat ctttacacca atcgctgcaa
      181 cagaccttgg ttttgaacca ggtatcccag ttatcgaagc tgggccaatc ttattccgta
      241 tcccagcaat gagcgctcca gtatttgaca atattgaagc agctattaaa gaacacggat
      301 taagctaatt ctaatttgtt aacattcata taacataaaa aagggaggaa ctgttatgga
      361 tgcaatcttt gacctaatcg gaaaggtttt caatcccatc ttagaaatgg gtggacctgt
      421 catcatgttg atcattttga cagtattggc tttacttttt ggagtgaaat tctccaaagc
      481 gcttgaaggt ggtatcaaac ttgccatcgc tcttacaggt atcggtgcta tcatcggtat
      541 gctaaatggt gctttctcag catcacttgc aaaattcgtt gaaaacactg gtatccaatt
      601 gagtattaca gacgttggtt gggcaccact cgcaacaatc acttggggtt ctgcttggac
      661 actatacttc ttgctcatca tgttgattgt caacgtagtg atgcttgcta tgaagaaaac
      721 cgatacactt gatgtcgata tatttgatat ctggcacttg tctatcacag gtctcttgat
      781 taaatggtat gctgataaca atggtgtgag tcaaggggtt tcactcttta ttgctacagc
      841 agttatcgtc cttgtcggtg tgttgaaaat tatcaactct gacttgatga aacctacatt
      901 tgatgacctt cttaacgccc caagttcatc accaatgaca tcaactcaca tgaactacat
      961 gatgaaccca gttatcatgg ttttggataa gatttttgaa aaattcttcc caggccttga
     1021 taaatatgac tttgatgctg ctaaattgaa caagaaaatc ggtttctggg gatctaaatt
     1081 cttcatcggt ttcatccttg gtatcgttat cggtattatg ggaactccac atccaattgc
     1141 aggtgttgca gatgcagata aatggcgtct tgttatcaaa ggatggttgt ctcttggttt
     1201 gactgccggt gtatctttgg aactcttctc acttatcggt tcatggttca tcgcagccgt
     1261 agaaccacta tcacaaggta ttacaaacgt tgctactaaa cgtcttcaag gacgtaaatt
     1321 caatatcggt cttgactggc cattcatcgc tggtcgtgct gaaatctggg cttgtgccaa
     1381 cgtacttgca ccaatcatgt tgattgaagc agtgcttctt tcaaaagttg gaaatggtat
     1441 cttgccactt gcaggtatca tcgctatggg tgttactcca gctctcttgg ttgtaactcg
     1501 tggtaaattg ctccgtatga ttatcttcgg aacactcttg ttgccactct tccttctttc
     1561 aggtaccctt attgcaccat ttgcaacaga acttgctaaa ggtgtaggtg ccttcccaga
     1621 aggtgtgagc caaactcaat tgattactca ctctactctt gaaggaccaa tcgaaaaact
     1681 tcttggttgg acaattggta acactacaac tggtgatatc aaagcaatcc ttggtgcagt
     1741 agccttcctt gtattctata tcggtatctt tgcttggtac agaaaacaaa tgatcaaacg
     1801 taacgaagag tacgcagcaa aagcaaaata atgcgctcct ataaaatata aactcaatga
     1861 aaatcaaaga gcaaactagg aaactagcca caggttgctc aaaacacagt tttgaggttg
     1921 tagatgaaac tgacgaagtc agtaatcata tctacggcaa ggcgacgctg acgaagtttg
     1981 aattggattt tcgcagagtg taaattgttg aaactaggtt gtcataccat gtataccact
     2041 tgggctttgg tagtagctaa ctgcactaaa tataatataa ggagggtttt atggggaaag
     2101 gccattggaa tcggaaaaga gtttatagca ttcgtaagtt tgctgtggga gcttgctcag
     2161 taatgattgg gacttgtgca gttctattag gaggaaatat agctggagaa tctgtagttt
     2221 atgcggatga aacacttatt actcatactg ctgagaaacc taaagaggaa aaaatgatag
     2281 tagaagaaaa ggctgataaa gctttggaaa ctaaaaatgt agttgaaagg acagaacaaa
     2341 gtgaacctag ttcaactgag gctattgcat ctgagaagaa agaagatgaa gccgtaactc
     2401 caaaagagga aaaagtgtct gctaaactgg aagaaaaagc tccaaggata gaatcacaag
     2461 cttcaagtca agaaaaaccg ctcaaggaag atgctaaagc tgtaacaaat gaagaaatga
     2521 atcaaatgat tgaagacagg aaagtggatt ttaatcaaaa ttggtacttt aaactcaatg
     2581 caaattctaa ggaagccatt aaacctgatg tagacgtatc tacgtggaaa aaattagatt
     2641 taccgtatga ctggagtatc tttaacgatt tcgatcatga atctcctgca caaaatgaag
     2701 gtggacagct caacggtggg gaagcttggt atcgcaagac tttcaaacta gatgaaaaag
     2761 acctcaagaa aaatgttcgc cttacttttg atggcgtcta catggattct caagtttatg
     2821 tcaatggtca gttagtgggg cattatccaa atggttataa ccagttctca tatgatatca
     2881 ccaaatacct tcacaaagat ggtcgtgaga atgtgattgc tgtccatgca gtcaacaaac
     2941 agccaagtag ccgttggtat tcaggaagtg gtatctatcg tgatgtgact ttacaagtga
     3001 cagataaggt gcatgttgag aaaaatggga caactatttt aacaccaaaa cttgaagaac
     3061 aacaacatgg caaggttgaa actcatgtga ccagcaaaat cgccaatacg gacgacaaag
     3121 accatgaact tgtagccgaa tatcaaatcg ttgaacgagg tggtcatgct gtaacaggct
     3181 tagttcgtac agcgagtcgt accttaaaag cacatgaatc aacaagccta gatgcgattt
     3241 tagaagttga aagaccaaaa ctctggaccg ttttaaatga caaacctgcc ttgtacgaat
     3301 tgattacgcg tgtttaccgt gacggtcaat tggttgatgc taagaaggat ttgtttggtt
     3361 accgttacta tcactggact ccaaatgaag gtttctcttt gaatggtgaa cgtattaaat
     3421 tccatggagt atccttgcac cacgaccatg gggcgcttgg agcagaagaa aactataaag
     3481 cagaatatcg ccgtctcaaa caaatgaagg agatgggagt taactccatc cgtacaaccc
     3541 acaaccctgc tagtgagcaa accttgcaaa tcgcagcaga actaggttta ctcgttcagg
     3601 aagaggcttt tgatacttgg tatggtggca agaaacctta tgactatgga cgtttctttg
     3661 aaaaagatgc cactcaccca gaagctcgaa aaggtgaaaa atggtctgat tttgacctac
     3721 gtaccatggt cgaaagaggc aaaaacaacc ctgctatctt catgtggtca attggtaatg
     3781 aaataggtga agctaatggt gatgcccact ctttagtaac tgttaaacgt ttggtcaagg
     3841 ttatcaagga tgttgataag actcgctatg ttaccatggg agcagataaa ttccgtttcg
     3901 gtaatggtag cggagggcat gagaaaattg ctgatgaact cgatgctgtt ggatttaact
     3961 attctgaaga taattacaaa gcccttagag ctaagcatcc aaaatggttg atttatggat
     4021 cagaaacatc ttcagctacc cgtacacgtg gaagttacta tcgccctgaa cgtgaattga
     4081 aacatagcaa tggacctgag cgtaattatg aacagtctga ctatgggaat gatcgtgtgg
     4141 gttgggggaa aacagcaacc gcttcatgga cttttgaccg tgacaacgct ggctatgctg
     4201 gacagtttat ctggacaggt acggactata ttggtgaacc tacaccatgg cacaaccaaa
     4261 atcaaactcc tgttaagagc tcttactttg gtatcgtaga tacagccggc attccaaaac
     4321 atgacttcta tctctaccaa agccaatggg tttctgttaa gaagaaaccg atggtacacc
     4381 ttcttcctca ctggaactgg gaaaacaaag aattagcatc caaagtagct gactcagaag
     4441 gtaagattcc agttcgtgct tattcgaatg cttctagtgt agaattgttc ttgaatggaa
     4501 aatctcttgg tcttaagact ttcaataaaa aacaaaccag cgatgggcgg acttaccaag
     4561 aaggtgcaaa tgctaatgaa ctttatcttg aatggaaagt tgcctatcaa ccaggtacct
     4621 tggaagcaat tgctcgtgat gaatctggca aggaaattgc tcgagataag attacgactg
     4681 ctggtaagcc agcggcagtt cgtcttatta aggaagacca tgcgattgca gcagatggaa
     4741 aagacttgac ttacatctac tatgaaattg ttgacagcca ggggaatgtg gttccaactg
     4801 ctaataatct ggttcgcttc caattgcatg gtcaaggtca actggtcggt gtagataacg
     4861 gagaacaagc cagccgtgaa cgctataagg cgcaagcaga tggttcttgg attcgtaaag
     4921 catttaatgg taaaggtgtt gccattgtca aatcaactga acaagcaggg aaattcaccc
     4981 ttactgccca ctctgatctc ttgaaatcga accaagtcac tgtctttact ggtaagaaag
     5041 aaggacaaga gaagactgtt ttggtgacag aagtgccaaa agtacagacc attattggag
     5101 aggcacctga aatgcctacc actgttccgt ttgtatacag tgatggtagc cgtgcagaac
     5161 gtcctgtaac ctggtcttca gtagatgtga gcaagcctgg tattgtaacg gtgaaaggta
     5221 tggctgacgg acgagaagta gaagctcgtg tagaagtgat tgctcttaaa tcagagctac
     5281 cagttgtgaa acgtattgct ccaaatactg acttgaattc tgtagacaaa tctgtttcct
     5341 atgttttgac tgatggaagt gtacaagagt atgaagtgga caagtgggag attgccgaag
     5401 aagataaagc taagttagca attccaggtt ctcgtattca agcgaccggt tatttagaag
     5461 gtcaaccaat tcatgcaacc cttgtggtag aagaaggcaa tcctgcggca cctgcagtac
     5521 caactgtaac ggttggtggt gaagctgtca caggtcttac tagtcaaaaa ccaatgcaat
     5581 accgcactct tgcttatgga gctaagttgc cagaagtcac agcaagtgct aaaaatgcag
     5641 ctgttacagt tcttcaagca agcgcagcaa acggcatgcg tgcgagcatc tttattcatc
     5701 ctaaagatgg tggccctctt caaacctatg caattcaatt ccttgaagaa gcgccaaaaa
     5761 ttgctcactt gagcttgcaa gtggaaaaag ctgacagtct caaagaagac caaactgtca
     5821 aattgtcggt tcgagctcac tatcaagatg gaacgcaagc tgtattacca gctgataaag
     5881 taaccttctc tacaagtggt gaaggggaag tcgcaattcg taaaggaatg cttgagttac
     5941 ataagccagg agcagtcact ctcaaagcgg aatatgaggg agctaaaggc caagttgatc
     6001 tcactatcca ggccaatact gagaagaaga ttgcgcaatc tatccgtcca gtaaatgtag
     6061 tgacagattt acaccaagaa cctactcttc cgtcaacagt aacggttgag tatgacaaag
     6121 gtttccctaa agctcacaaa gtcacttggc aagctattcc gaaagaaaaa ctagactcct
     6181 atcaaacctt tgaagtacta ggtaaagttg aaggaattga ccttgaagcg cgtgcaagag
     6241 tctctgtaga aggtatcgtt tcagttgaag aagtcagtgt gacaactcca atcgcagaag
     6301 caccacaatt accagaaagc gttcggacat atgattcaaa tggtcacgtt tcatcagcta
     6361 aggttgcatg ggatgcgatt cgtatagagc aatacgctaa ggaaggtgtc tttacagtta
     6421 atggtcgctt agaaggtact caattaacaa ctaaacttca tgttcgcgta tctgctcaaa
     6481 ctgagcaagg tgcaaatatt tctgaccaat ggaccggttc agaattgcca cttgcctttg
     6541 cttcagactc aaatccaagc gacccagttt caaatgttaa tgacaagctc atttcctaca
     6601 ataaccaacc agccaatcgt tggacaaact ggaatcgtag taatccagaa gcttcagtcg
     6661 gtgttctgtt tggagattca ggtatcttga gcaaacgctc cgttgataat ctaagtgtcg
     6721 gattccacga agaccatgga gttggtgcac cgaagtctta tgtgattgag tattatgttg
     6781 gtaagactgt cccaacagct cctaaaaacc ctagttttgt tggtaatgag gaccatgtct
     6841 ttaatgattc tgccaactgg aaaccagtta ctaatctaaa agcccctgct caactcaagg
     6901 ctggagaaat gaaccacttt agctttgata aagttgaaac ctatgctgtt cgtattcgca
     6961 tggttaaagc agataacaag cgtggaacgt ctatcacaga ggtacaaatc tttgcgaaac
     7021 aagttgcggc agccaaacaa ggacaaacaa gaatccaagt tgacggcaaa gacttagcaa
     7081 acttcaaccc tgatttgaca gactactacc ttgagtctgt agatggaaaa gttacggcgg
     7141 tcacagcaag tgttagcaac aatggtctcg ctaccgtcgt tccaagcgtt cgtgaaggtg
     7201 agccagttcg tgtcatcgcg aaagctgaaa atggcgacat cttaggagaa taccgtctgc
     7261 acttcactaa ggataagagc ttactttctc ataaaccagt tgctgcggtt aaacaagctc
     7321 gcttgctaca agtaggtcaa gcacttgaat tgccgactaa ggttccagtt tacttcacag
     7381 gtaaagacgg ctacgaaaca aaagacctga cagttgaatg ggaagaagtt ccagcggaaa
     7441 atctgacaaa agcaggtcaa tttactgttc gaggccgtgt ccttggtagt aaccttgttg
     7501 ctgagatcac tgtacgagtg acagacaaac ttggtgagac tctttcagat aaccctaact
     7561 atgatgaaaa cagtaaccag gcctttgctt cagcaaccaa tgatattgac aaaaactctc
     7621 atgaccgcgt tgactatctc aatgacggag atcattcaga aaatcgtcgt tggacaaact
     7681 ggtcaccaac accatcttct aatccagaag tatcagcggg tgtgattttc cgtgaaaatg
     7741 gtaagattgt agaacggact gttgcacaag gaaaagttca gttctttgca gatagtggta
     7801 cggatgcacc atctaaactc gttttagaac gctatgtcgg tccagagttt gaagtgccaa
     7861 cctactattc aaactaccaa gcctacgacg cagaccatcc attcaacaat ccagaaaatt
     7921 gggaagctgt tccttatcgt gcggataaag acattgcagc tggtgatgaa atcaacgtaa
     7981 catttaaaga tatcaaagcc aaagctatga gatggcgtat ggagcgtaaa gcagataaga
     8041 gcggtgttgc gatgattgag atgaccttcc ttgcaccaag tgaattgcct caagaaagca
     8101 ctcaatcaaa gattcttgta gatggaaaag aacttgctga tttcgctgaa aatcgtcaag
     8161 actatcaaat tacctataaa ggtcaacggc caaaagtctc agttgaagaa aacaatcaag
     8221 tagcttcaac tgtggtagat agtggagaag atagccttcc agtacttgtt cgcctcgttt
     8281 cagaaagtgg aaaacaagtc aaggaatacc gtatccactt gactaaggaa aaaccagttt
     8341 ctgagaagac agttgctgct gtacaagaag atcttccaaa actcgaattt gttgaaaaag
     8401 atttggccta caagacagtt gagaaaaaag attcaacact gtatctaggt gaaactcgtg
     8461 tagaacaaga aggaaaaact ggtaaagaac gtatctttac agcgattaat cctgatggaa
     8521 gtaaggaaga aaaactccgt gaagtggtag aagctccgac agaccgcatc gtcttggttg
     8581 gaaccaaacc agtagctcaa gaagctaaaa aaccacaagt gtcagaaaaa gcagatacaa
     8641 aaccaattga ttcaagtgaa gctagtcaaa ctaataaagc ccagttacca aatacaggta
     8701 gtgcgtcaag ccaagcagca gtagcagcag gtttagctct tctaggtttg agtgcaggat
     8761 tagtagttac taaaggtaaa aaagaagact agaaattttg ataccttctt tatcattaaa
     8821 gaccaaagaa aaactcttgt cctaaataga agataaagag tctggaacaa aatagttctc
     8881 aaccacaaaa aagctagata tttccaattg cggaactcta gctttttaat tttgagtcgt
     8941 tctctgattg tatagtagat tgaaactaga gtagtacact tctacttcta aaacattgtt
     9001 agaaatcgat ttgactgtcc ttatcgattt gtcctgttct tatttgattt cactatgttt
     9061 taggaggtta ttggccataa gtaccaatcc catgtcaatt ctcacttgac gcttgcctct
     9121 cagattacat ctcttgtaac ccaaacaagc ctttatctgc ccaaagacag gttccacatc
     9181 aatcttgcgt tgagcgaaaa tctgtctatc ttggggagat aaaagcgcct gacattcttt
     9241 agctttcaag ttttgatagc gttcgttcat atacagtccc ttttgagggg ctgattcagg
     9301 ttcatcagcg tagtaaacct tgatttcctg ttgaaagtct gtctgtgttt tctggtactt
     9361 gatatggtga aaacgatagc accagccatc aggatgtatg tagctatcct ccttgtcatt
     9421 atagtgccaa ttcgctaagt ttttagctga ctgtttatac cctctctttt gtttcttatc
     9481 aaacatggca tatttaatca gaaggggagt gatttcttct tgaaaataga cttgtatctg
     9541 ttcttgaagt ttggcggaga atttctctgt cgttttcttc cacacaaaac tatacttgtt
     9601 ggcattggct tcaatcttag ttccgtcaat aaacaaacaa tctaaggtca ctaactcttc
     9661 catttttcaa tttttcgacc agagaaaatc ccttgtgaat aggcaaatag aagagtagat
     9721 acaagcattt taaggtgata agacgggcga tcaaaggcat gatagaaggc gtggaaatga
     9781 caatcctcca aggtattcac cactttttca atagtaaaaa cgagatggtc ttgtggcaag
     9841 aaggaactga tttctagtgg taaagttgtt tgatttgtgt tatagtgaat atgcatggga
     9901 tagcctttct aaatggttta ttgtgcaatt ctattttacc aagactatcg caaccatgca
     9961 aaaaagcaac ggcaaatccg ttgctttttt tggagataag agactattgt cccagactct
    10021 tttctttgta taaaattgta tagtgttatt gtctatacta ttttgtagag aaagcaaaaa
    10081 tccctagaaa atgcttaggg attcgactat ttcattaaga acaattcaac aatcatttct
    10141 acatacatga taagggttaa taagaatcct gcacgccaga agaatttgat gaatttagga
    10201 tagtaaaagc tgcgtttttt caaaaggaag aaaaagacga gaataatggc taggatcgag
    10261 agggttagcc ccagtctagg gaggaaatta tgggtaaagg ttttagctgt aatcaagtaa
    10321 tactcaaata ccaagactgg aaaagccaaa tccgcaaagt ttcgtcctag tttttttaat
    10381 ctaaaaagtt tggttaggat aatgcagatg actaaggtca gtatcaataa taaaatagat
    10441 gctaatttca ttaaaatcat acccatattg tatcataaaa aaaggctaat ggaaacgaga
    10501 aacagaggaa tttataagaa agtcaggctt ttgagattgt gggtgatttt tgttataatg
    10561 aaagttataa aatcttataa taccttgaat gattattatc gaaaactctt tggagaaaag
    10621 acttttaaag tccctattga tgcgggattt gactgtccca atcgtgatgg aactgtggct
    10681 catggaggct gtactttttg tacggtttca ggttctggag atgctattgt agccccagat
    10741 gcgcctatcc gtgagcaatt ttataaggaa attgacttta tgcaccgtaa gtggccggat
    10801 gttcagaagt atctggttta ttttcaaaat tttaccaaca ctcatgaaaa ggtggaagtt
    10861 attcgagagc gttatgaaca ggctatcaat gagccaggtg tggtaggaat caatattgga
    10921 acgcgcccag actgtttacc agacgaaacc atcgaatatt tggctgagtt atcggagtgc
    10981 atgcatgtga cgtttgaatt gggcttgcaa actacttatg aagcaacctc tgacctgatt
    11041 aaccgtgcgc attcttatga attgtaggtg gaaacggtca agcgtttgag aaagtatcca
    11101 aaaattgaga ttgtttccca tttgatcaat ggtttgcctg gtgaaaccca tgagatgatg
    11161 gttgaaaatg tccgccgttg tgtcacggat aacgatattc aagggattaa actgcatttg
    11221 ctccatctta tgacaaatac tcgtatgcaa cgagattacc atgaggggcg tttacaattg
    11281 atgagtcagg acgaatatgt cagggtcatc tgtgaccaac tggaaattat tcccaaacat
    11341 atcgtcatcc atcgaattac aggagatgcg cctagggata tgctgcttgg gcctatgtgg
    11401 agcctcaata aatgggaagc tctcaacagc attgagatgg agatgcgacg tcgtggaagt
    11461 gttcaaggat gcaaggctgt aaaacaggag tttgaaaatg aaaagaccac ttgagatggc
    11521 acatgatttt ttggctgagg tcgtgacaaa agaggatgtc gtagtggatg cgactatggg
    11581 aaatggtcat gacacgcttt ttttagccaa gctagccaag caagtctatg cctttgatat
    11641 tcagaagcaa gccttggaaa agacccaaga gcgtttgcat caggctgact tgacaaatgc
    11701 ccagttaatc ttgcaaggcc atgagacact ggaccagttt gtgataaaag ctaaggcagg
    11761 gatttttaat ctgggctatt tgccgtcagc tgataagtct gtcatcaccc gaccgcagac
    11821 aacgattgag gcattagaaa agctatgtgg cttacttgtc aaaggtggac gaattgctat
    11881 tatgatttac tatggtcatg aaggaggcga tctcgagaga gatgctgtct tggattttgt
    11941 gatccagttg aaccaacaag agtacacagc tgccatttac cgaactttaa accaagtcaa
    12001 caacccgccg tttttagtga tgattgaaaa attagagaga tacagacatg gataaacaat
    12061 acttacacga aaagctggat gccatgcgcc agaattttgt tgaatcaacc caccacgaac
    12121 gagcggtggg cgtgctagac caagcgcata tgagcaaaaa aatgcttaaa atcaagaaaa
    12181 aattagttgc tcttgaaatg gaacggtgcc agagaaaaat tgagcacaaa gactgttcca
    12241 agattgacca aaaaatcaaa gagcagaagg agatatttga atcctgttgt aaaaaagatt
    12301 aaggaggacg tgcgtggaat tactgattta cctcatccta tttttactgg ttttgattgt
    12361 ctcaagtaca accaataagc tcctgccctt tttgcctctc cctttggtgc aaattctttt
    12421 gggaattgtg attggtctct ttttacccaa tactgacttt caccttaata cggagttgtt
    12481 tttggcactg gttatcggac ccttgctttt ccgagaggct gaagaagcag atgttacggc
    12541 tattttaaaa cactggcgaa tcattgttta tctcatattt ccagtgattt ttatctcgac
    12601 cctgagtttg ggtggcttgg cccatcttct ttggttcagc cttcccttgg cagcttgctt
    12661 ggctgttggg gcagcccttg gtcctacgga cttggtggcc tttgcctctc tttcggagcg
    12721 ttttagcttt cctaagcgcg tgtccaatat tcttaagggc gaaggactct tgaatgatgc
    12781 ttctggtttg gtggcttttc aggtagcttt gacagcttgg acaactggag ctttttctct
    12841 ggggcaagct agcagttcgc tcatcttttc aatcctaggc ggttttttaa ttggattttt
    12901 aacagccatg accaaccgct tcctccatac cttcttgcta agtgtgcgcg caacggatat
    12961 tgccagtgaa cttttattag aattgagttt gcctctagtg accttctttc tggcagaaga
    13021 agtccatgtt tcaggtatta ttgccgtcgt agttgctgga attttaaagg caagtcgctt
    13081 caagaaaatc acgctcctcg aagcccaagt ggatacggtg accgagacgg tctggcatac
    13141 agtgaccttt atgctcaacg gttctgtctt tgtgatttta gggatggagt tggaaatgat
    13201 agcagaacct atcttgacca atccaatcta taatcctcta cttttattgc tatctctcat
    13261 cgcccttacc tttgtcctct ttgtcattcg ttttattatg atctatggct attatgccta
    13321 tagaacccga cgcctcaaga aaaagctaaa taagtatatg aaggacatgt ttctcttgac
    13381 cttttcaggt gttaagggaa cggtgtcgat tgctacgatt ctcttgatac caagtaatct
    13441 agaacaggag tatcctcttt tgcttttcct tgttgcaggt gtgacgcttg tcagcttttt
    13501 aacaggtctc ttggtcttgc ctcatctttc tgatgaagag gaaaaaagca aggattatct
    13561 catgcatatc gccattttga atgaagtaac gctagagttg gaaaaagagt tggaagacac
    13621 cagaaataaa cttcccctct atgcggctat tgacaattat catggacgta ttgaaaatct
    13681 cattttaagc caagaaaacc aggatgatca agaagactgg gctgctttga aactcttgat
    13741 tcttagtatt gaaagtgatg gtttggaaca ggcctatgaa gaggggaaca ttagcaatcg
    13801 tgcttaccga gtttaccaac gttatctgaa aaatatagaa caaggaatca atcgtaaact
    13861 tgcctcaaga ctgacctatt atttccttgt ttccttgagg attttacgtt ttctccttca
    13921 tgaagttttt actcttggaa agaccttccg tagctggaag gacaaggagc aaagccgtct
    13981 ccgtgctctt gattatgacc aaattgcaga gctctatctt gccaatacgg agatgattat
    14041 tgaaagtttg gaaaacctga agggagtcta cagacgctct ttgattagtt ttatgcagga
    14101 gtctcgtctt cgagaaacag ctattatcag cagtggtgcc tttgtcgaac gggttatcaa
    14161 tcgtgtcaaa cccaacaata tcgatgaaat gctgagaggc tattatctgg agcgcaagtt
    14221 gattttcgaa tacgaagaaa aacgattaat tacaactaag tatgccaaga aattacgaca
    14281 aaatgtaaat aacttagaga actattcctt gaaggaagct gccaataccc tgccgtatga
    14341 tatggtggaa ttggtaagaa gaaattagtt aatactcttc gaaaatctct tcaaaccacg
    14401 tcagcgtcgc cttggattat atatgtgact gacttcgtca gtttcatcta caacctcaaa
    14461 gcagggcttt gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta
    14521 taagattata aatgaaggag tatgacatga aaaagtggtg gaaagagctg atagacaagc
    14581 ctttattaaa agcttttttg cattattatc aagcatcaga tagtgagttg accagtgtcg
    14641 cagtagccta ctattggttg atttcgattt tccccctgct tttggtggtg gtcaatatcc
    14701 tcccctattt tcagattcct gtgggagaat ttctgtgctt tatgaaagac gttctgcccc
    14761 caagcctcta tgaaggtgtg gaaaaaatag ccagagaagt tttgacgcaa ccttcaacag
    14821 gtttattaag tttttctgtt ttatcggcgt tatggagttt ttcaaaatct atgaatttct
    14881 tgcaaaaagc ttttaacaag gcttatggcg tcgaaaagag tcgtggactt atttcccatc
    14941 agatgctaag tctcctagtc agttttggct tgcagctcct ctttgcgttt gccttgtttt
    15001 tgatcttatt tggtcagatg attttggctt tacttgctca ttattggaca aaagatggca
    15061 ttatctatca ggctttacaa gggttggcag gtcctctgat ttacgccctc ctctttgcta
    15121 tcttggtcat gctttattat tttcttccca atatatctaa caggaaaatt agctataccc
    15181 taccaggcag tgcctttgtt ctcttagtga ttttaggttt actaactctg ttctctagtt
    15241 atctcaatta ctatgtccat catttggtgg atgtccgaat tttaggttcc gttcttcttg
    15301 ttgttatgat gttttggttt atcttgattg ctaaaattgt tatcctaggc gcggttatca
    15361 atgccagtat gcagagtttg aaagatccgg tctttaaaaa agactaacta tttatccatt
    15421 ataaaagcgc atagtatcaa gactttatag aaccttgata ctatgcgctt ttgcgctttt
    15481 gtgctttgac agttttttgt atagtttttg tccaagattt ctaaatcatc tgattaaatt
    15541 cctattttcc cctatcattg tccctgtttt ttcgaaaatg gaaggagtat aataaatcta
    15601 tcaataaaat gataggagga actttatggg tcatattttc ttttttctaa gtgtcttttt
    15661 ggcagggatt ctatccttct tttctccttg tatcttacct ttgttaccgg tctatacagg
    15721 agtgttacta gatgataagg atggtgctca ggcttctagc ggcaaatttt caatctcagt
    15781 tactagttta ttacgaactc tggcctttat agcaggaatt tcctttatat ttattttgtt
    15841 gggctatgga gctggttttt taggcgattt gctttatgct tcttggttcc aatatcttac
    15901 tggggcaatt attatccttc ttggtttgca ccaaatggag attctacact ttaaggggct
    15961 ttataaggaa aagaggctac aactgcaagg acaggggcaa aatggtaagg gctatagtca
    16021 ggcattttta ttgggcttga cctttagttt tgcttggacg ccttgcgtgg ggccggttct
    16081 ggggtctgtt ttggccttgg cggcttcagg tggttcagga gcttggcagg gagctggtct
    16141 catgttggtg tatacgctgg gcttggcgct accattcttg cttctagctc tgacctctag
    16201 ttatgttttg aaacatttcc gaaaacttca tccctatctc ggaatcctca aaaaagtggg
    16261 tggttttctc attattgtga tgggactctt ggttctgttt ggaaatgctt caattttaag
    16321 tcaattattt gaataaaatg gaaaggaata tcaatatgaa aaaatggcaa acatgtgttc
    16381 ttggagcagg ttcgctcctt tggttgacgg cttgttcagg caagtccgtg actagtgaac
    16441 accaaacgaa agatgaaatg aagacggagc agacagctag taaaacaagc gcagctaaag
    16501 ggaaagaggt ggctgatttt gaattgatgg gagtagatgg caagacctac cgtttatctg
    16561 attacaaggg caagaaagtc tatctcaaat tctgggcttc ttggtgttcc atctgtctgg
    16621 ctagtcttcc agatacggat gagattgcta aagaagctgg tgatgactat gtggtcttga
    16681 cagtagtgtc accaggacat aagggagagc aatctgaagc ggactttaag aattggtata
    16741 agggattgga ttataaaaat ctcccagtcc tagttgaccc atcaggcaaa cttttggaaa
    16801 cttatggtgt ccgttcttac ccaacccaag cctttataga caaagaaggc aagctggtca
    16861 aaacacatcc aggcttcatg gaaaaagatg caattttgca aactttgaag gaattagcct
    16921 aggaggtgtc ttatgaatga taagttaaaa atcttcttgt tgctaggagt attttttcta
    16981 gccataaccg gtttctatgt tctattgata cgaaatgcag ggcagacaga tgcctcgcaa
    17041 attgaaaagg cggcagttag ccaaggagga aaagcagtga aaaaaacaga aattagtaaa
    17101 gacgcagact tgcacgaaat ttatctagct ggaggttgtt tctggggagt ggaggaatat
    17161 ttctcacgcg ttcccggggt gacggatgcc gtttcaggct atgcaaatgg tagaggagaa
    17221 acaaccaagt acgaattgat taaccaaaca ggtcatgcag aaaccgtcca tgtcacctat
    17281 gatgccaagc aaatttctct caaggaaatc ctgcttcact atttccgcat tatcaatcca
    17341 accagcaaaa ataaacaagg aaatgatgtg gggacccagt accgtactgg tgtttattac
    17401 acagatgaca aggatttgga agtgattaac caagtctttg atgaggtggc taagaaatac
    17461 gatcaacctc tagcagttga aaaggaaaac ttgaagaatt ttgtggtggc tgaggattac
    17521 catcaagact atctcaagaa aaatccaaat ggctactgcc atatcaatgt taatcaggcg
    17581 gcctatcctg tcattgatgc cagcaaatat ccaaaaccaa gtgatgagga attgaaaaag
    17641 accctgtcac ctgaggagta tgcagttacc caggaaaatc aaacagaacg agctttctca
    17701 aaccgttact gggataaatt tgaatccggt atctatgtgg atatagcaac tggggaacct
    17761 ctcttttcat caaaagacaa atttgagtct ggttgtggct ggcctagttt tacccaaccc
    17821 atcagtccag atgttgtcac ctacaaggaa gataagtcct acaatatgac gcgtatggaa
    17881 gtgcggagcc gagtaggaga ttctcacctt gggcatgtct ttacggatgg tccacaggac
    17941 aagggcggct tacgttactg tatcaatagc ctctctatcc gctttattcc caaagaccaa
    18001 atggaagaaa aaggctacgc ttatttacta gattatgttg attaatactc ttcgaaaatc
    18061 tcttcaaacc acgtcagcgt cgccttaccg tatgtagggt tactgacttc gtcagtttta
    18121 tctacaacct caaagctata ctttgagctg acttcgtcag ttttatctac aacctcaaag
    18181 ctatactttg agctgacttc gtcagtttta tttacaacct caaagctata ctttgagaaa
    18241 cctgtggcta tcttcctagt ttactttttg atttttattg agtataagag gtctttccta
    18301 agcagttaga ggagaatttt gctatactga tactagtaag tggtaaagga gcagagcatg
    18361 acctacacaa tcttaatcgt agaagatgaa tatctggtaa gacaaggttt gactaaactg
    18421 gtcaatgtag cagcctacga tatggaaatc atcggtcagg ctgaaaatgg aaggcaggct
    18481 tgggaattga tccaaaagca ggtgccagat atcattttaa ccgatatcaa catgcctcat
    18541 ctaaatggca tccagttggc cagtctggta cgagaaacct atcctcaggt tcatttggtc
    18601 tttttaacag gttacgatga ttttgattat gccttgtctg ctgtcaaact aggtgtggac
    18661 gactacctgc tcaaaccctt ttctcgtcag gatattgagg aaatgttggg gaaaatcaaa
    18721 caaaaactag acaaggaaga gaaagaagag cagttacaag atttattgac taataggttt
    18781 gaaggaaaca tggcccagaa aatccagtct catctggctg atagccaatt tagtttaaag
    18841 tctttagcca gtgacttagg ttttagtccg acctatctga gttccttgat taagaaagag
    18901 ttgggcttgc cttttcagga ttatctggtg agagaacgtg ttaaacaagc caagctcttg
    18961 cttttaacta cagatctgaa gatttatgag atcgcagaga aggttggttt tgaagatatg
    19021 aactatttta cccaacgttt taagcagatt gcaggtgtga cacctcgtca gtttaagaag
    19081 ggagaagacc gatgaagcgt tcttctcttt tagttagaat ggttatttcc atctttctgg
    19141 tctttctcat tctcctagct ctggttggaa ctttctacta tcaatcaagt tcttcagcca
    19201 ttgaggccac cattgagggc aacagccaaa cgaccatcag ccagactagc cactttattc
    19261 agtcttatat caaaaaacta gaaaccacct cgactggttt gacccagcag acggatgttc
    19321 tggcctatgc tgagaatccc agtcaagaca aggtcgaggg aatccgagat ttgtttttga
    19381 ccatcttgaa gtcagataag gacttgaaaa ctgttgtgct ggtgaccaaa tctggtcagg
    19441 tcatttctac agatgacagt gtgcagatga aaacttcctc tgatatgatg gctgaggatt
    19501 ggtaccaaaa ggctattcat cagggagcta tgcctgtttt gactccagct cgtaaatcag
    19561 atagtcagtg ggtcatttct gtcactcaag aacttgttga tgcaaaggga gccaatcttg
    19621 gtgtgcttcg tttggatatt tcttatgaaa ctctggaagc ctatctcaat caactccagt
    19681 tggggcagca gggctttgcc ttcattatca atgaaaacca tgaatttgtc taccatcctc
    19741 aacacacagt ttatagttcg tctagcaaaa tggaggctat gaaaccctac atcgagacag
    19801 gtcagggtta tactcctggt cacaaatcct acgtcagtca agagaagatt gcaggaactg
    19861 attggacggt acttggcgtg tcatcattgg aaaagttaga ccaggttcgg agtcagctct
    19921 tgtggacctt gcttggggcc agtgtcacat ctcttcttgt ttgtctctgc ttagtgtggt
    19981 tcagtcttaa acgctggatt gctcctttga aggatttgag agaaaccatg ttggaaattg
    20041 cttctggtgc tcaaaatctt cgtgccaagg aagttggtgc ctatgaactg agagaagtaa
    20101 ctcgccaatt taatgccatg ttggatcaga ttgatcagtt gatggtagct attcgtagcc
    20161 aggaagaaac gacccgtcag taccaacttc aagccctttc gagccagatt aatccacatt
    20221 tcctctataa cactttggac accatcatct ggatggctga atttcatgat agtcagcgag
    20281 tggtgcaggt gaccaagtcc ttggcaacct atttccgctt ggcgctcaat caaggcaaag
    20341 acttgatttg tctctctgac gaaatcaatc atgtccgcca gtatctcttt atccagaaac
    20401 aacgctatgg agataagctg gaatacgaaa ttaatgaaaa tgttgccttt gataatttag
    20461 tcttacccaa gctggtccta caaccccttg tagaaaatgc tctttaccat ggcattaagg
    20521 aaaaggaagg tcagggccat attaaacttt ctgtccagaa acaggattcg ggattggtca
    20581 tccgtattga ggatgatggc gttggcttcc aagatgctgg tgatagtagt caaagtcaac
    20641 tcaaacgtgg gggagttggt cttcaaaatg tcgatcaacg gctcaaactt cattttggag
    20701 ccaattacca gatgaagatt gattctagac cccaaaaagg gacgaaagtt gaaatatata
    20761 taaatagaat agaaactagc taactcccag tcaattctgg gagttttatg cgtaattggg
    20821 caagctttct aggttgtcta tcttgctaaa aacaagaaaa aacggtatga tagaagagta
    20881 acaacagagg taacagagat gaacgagaca cagattcaaa gagaaacacg tcaggtagtg
    20941 gaagatgttt tagaaaagac caatttgaag cagggggtcc tttttgtttt aggtctatct
    21001 tctagtgagg tgctaggtgg tcagattggg aaagaatcca gtcaagagat tggtgagctg
    21061 atagtggaga ctatcttggg tattctaggt agtagaggta tccatttagc agttcaaggt
    21121 tgtgagcatg tcaatcgggc tctcgttgtt gaacgtcagg tggcagagca gtttgatctg
    21181 gaaattgtca gtgtccatcc tactcttcat gcaggaggtt cggggcagtt ggcagccttt
    21241 aagtttatgc aggatccagt tgaggttgaa tttatcaagg ctcatgctgg attggatatc
    21301 ggagacactg caattggcat gcatgtcaag catgttcagg ttccgattcg ccctattttg
    21361 agagagattg gtcatgccca tgtaacggca ctggctagtc gtccaaaatt aatcggaggt
    21421 gcgcgtgcgc actatccgca agatgctatt agaaagtctt gagaatgaat aaaggagata
    21481 aaagtgttta aaaaagaccg tttttcaatt cgtaagatta agggagttgt aggctctgta
    21541 tttcttggaa gccttttgat ggctccttct gtagtggacg cagccaccta tcactatgta
    21601 aataaagaga ttatttcaca agaagctaaa gatttaattc agacaggaaa gcctgacagg
    21661 aatgaagttg tatatggttt ggtgtatcaa aaagatcagt ttcctcaaac agggacagaa
    21721 gcatctgttt tgacagcttt tggtttgctg actgttggga gcttgctttt aatctacaag
    21781 agaaagaaaa ttgctagcgt ctttctagtt ggagctatgg gattggtagt tcttcctagt
    21841 gcaggggctg tagacccagt tgcgacccta gcgctggcta gtcgagaggg tgttgttgaa
    21901 atggatggct atcgctatgt tggttatcta tcaggtgaca tcctcaaaac gcttggcttg
    21961 gacactgttt tagaagaaac ctcagcaaaa cctggagagg tgactgtagt cgaagttgag
    22021 actcctcaat caacaacaaa tcaggagcaa gctaggacag aaaaccaagt agtagagaca
    22081 gaggaagctc caaaagaaga agcacctaaa acagaagaaa gtccaaagga agaaccaaaa
    22141 tcggaggtaa aacctactga cgacaccctt cctaaagtag aagaggggaa agaagattca
    22201 gcagaaccag ctccagttga agaagtaggt ggagaagttg agtcaaaacc agaggaaaaa
    22261 gtagcagtta agccagaaag tcaaccatca gacaaaccag ctgaggaatc aaaagttgaa
    22321 ccaccagtag aacaagcaaa agtcccagaa caacccgtgc aacctacaca agctgagcaa
    22381 ccaagtacac caaaagaatc atcacaacaa gaaaatccta aagaagatag gggagcggaa
    22441 gagacaccga aacaagaaga tgaacagcca gcagaagccc cagaaatcaa ggttgaagaa
    22501 ccagtagaat caaaagagga gactaaaaca gctaaaggta cgcaagaaaa aggtcacgtg
    22561 ggtgaggctc ttgctcaaga acaattacct gagtacaaag taactgaggg aacccttgtt
    22621 gaaacatcga ctacagacct agactacaaa actgaaacga ctgaggatcc aaccaagtat
    22681 acggacgaag aaactgtcct acgaactggt gaaaaaggaa gtcaagttac aaaaacaacc
    22741 tataaaacag tagaaggtgt taaaactgac caagttcttt caaccagtac ggaagtgacc
    22801 aaggagcctg taaatcaaca ggtaatccgt ggtacaaaac caattgaagg aactcttata
    22861 gaagaaagtc ttgaaaagat tccatttaag gaagttgtta aagaagatga ccaactgaaa
    22921 aaaggcttag aagttgtagc tcaagaagga aaagaaggcc agaaaaaaat caccaagacc
    22981 tttaatacca ttaagggagt taaaacagaa gacgctccaa aagttacaga ggaaatcctt
    23041 gaggaacctc aagacaaaat tttgaaacgt ggtaccaaga gctttgaaaa accagtgttg
    23101 accatcacag aaattgaatc taaggatttg aagcgtactt cagatgttaa gtatagttta
    23161 gaaaatccaa gtaaggcagc cattaaatct attaccctaa ctctgaaaaa aggtgatgag
    23221 attgtcaaaa ccttgaatgt ttcaccagaa gacttgacaa ctaccctgac agatttgcag
    23281 tactacaagg attataaact tgaaacaaag atggtttatg accgtggtga gggggatgag
    23341 gaagaagttc tgaaagaaga accgctaaga attgatctca aaaaagttga aataaaagat
    23401 attataagaa cggatttagt caaatatgaa aaccaagttg aaactgatga aactcgattg
    23461 acttcagtgc caactgattt gtcaaattat tatttaaaag ttacctctaa tgaccataag
    23521 accaatctac tagcggttca aaaaattgaa gagtcaacaa ttgatggaaa acaagtctac
    23581 aaggtaatag cagtagcgga taacttggtc caacgtaatg tcgataatcg ctttgaagaa
    23641 acttatacct actatattga aaaacccaaa gctagtcagg ctggtatttt ctacgacttt
    23701 gaagaattga tccaagccat tcagacaaat cctgccggtg aatttaaact gggtcaaagc
    23761 atgagcgctc gtaacgttgt tcctaaaggg aaatcttata ttacttcaga gtttactggt
    23821 aaactattga gtgatggtga taaacgctat gctatccata atttggaaca tcctttattc
    23881 gataagatta aggctggaac tgttaaaaat ataaattttg aaaatattga tattgattta
    23941 tctaatcaag ataatatcgc tgcaatagca aatgaaatca gtggaacgac acgtatagaa
    24001 aatatcaaag caatcggaac cataacagct agaaataatg ttgctgggtt cgtaaataac
    24061 ataaaagaag acgctacttt agaaaatgtg gcatttatcg gtagtataaa ttctgctggt
    24121 aataattcaa cagttggagg tattgccggt tctaactata tggggtttgt taatagagcc
    24181 tacgttgatg ctactattac agcaaataac gcaaatgcaa gtatgcttgt tcctttcgtt
    24241 caatataaag ctcatagctg gctatcaggg acaaaaggaa aacttacaaa ctctgtagca
    24301 aaaggtattc ttgatgttaa aaatactaaa tatgttggtg gaataacctc taaaacttgg
    24361 ccatatggag cggttcaaga taatgtaaca tatgcgaaag ttattaaagg tcaggaaata
    24421 ttcggttcaa acgatgttaa tgatggagat gcaaatcctt ttgtgtctaa tttatttgga
    24481 gttattggat atagctcttc tgaagatgga actggtaaag ataataaaaa caaaaataaa
    24541 ttaaaacact tatctaaatc tgaggctgat gcaaaagttg ctagtttcaa tattactgct
    24601 gctaaattcg taagtgaacc ttatgaattg aacaaattga ataatgttag cagtcgtgaa
    24661 gcagcttacg agtctattca agattataat gcagactata aacttgctta taagaatatt
    24721 gaaaaactac aaccattcta taacaaagat tttatcgtta atcaagctaa taaactagat
    24781 aagaacaaca agttaaatac taaagagatt ctatcggtta ctcctatgaa caataataac
    24841 tttgtaactg atttaggtga tgcaaacaaa attatcgttc attatgctga tggaacaaaa
    24901 gactacttca acctatcttc tagtgatgaa ggtctaagta atgttagaga atacactata
    24961 actgacttag gtattaaata tacacctaat atcgttcaaa aagatcatag tgctttaatt
    25021 aacggtatcg taaatatctt aagccctatt gagcttcaat ctaatccaat ttaccaaaaa
    25081 cttggcagaa cagggtctaa tagagtaaat gcaattaaag acctttattt agaagaaagt
    25141 tttgaccatg taaaagctaa tttaaacata ttagtaacta aattagttga aaatgaagat
    25201 catcaactga ataattcatc tgcagctcaa caaatgattc ttgataaagt tgagaaaaat
    25261 aaagctgccc tattattagg tttaacatac ttaaaccgtt actacggtgt taaattcggt
    25321 gatgttaaca ttaaagaatt aatgttattc aaaccagatt tctatggtaa aaatgtaagt
    25381 gtattagata gattaattga aatcggttca agagaagata atataaaagg tcatcggaca
    25441 tatgatgctt tcagcgagat tatttctaag acaaccttat cagaaaattt ggtaaacttt
    25501 ttaaattata atcgtcgact atttactgca gatgtaaata tttatgattg gtttaagaaa
    25561 gctacagaag ggaatgtcta tatagcggag cgtgcttcgg aagtcgatga aattaaagcg
    25621 gcgaaatata aagtttggga taatttacaa tctgctactc atcggaagat gatactccca
    25681 ttattaaatc taaataaatc acatgtgtat ttaatctcga attatagtgc aatggctgtt
    25741 ggaacagcag aaaagttagg gcatagctcc ttcgatggaa taaaaaatca gattgaaaaa
    25801 gcagcggaca gttatcgaaa ttattttgat ttttggtata gattatcttt agataaggtt
    25861 aagaatatgc tagtcaaaaa tacagtaact cctctttggg aaggttatca agcacctggt
    25921 ggatggcctg ataagtatgg taagagaaat aataaagaag actatgcccc attgagagag
    25981 ttgtttggac ctattggtaa gtattatggt aacaatggaa caggtgctta tgcagttatt
    26041 tgggataatc ctttggatac gcgaacagaa gtaaattata ttgcattaag tatgattgat
    26101 gagtttggaa tctctgttta tacacatgaa actactcatg ttaatgatcg tgcaatctat
    26161 ttaggaggat atggacgtcg ctcagggact catgcagaag cctatgcaca aggaatgtta
    26221 caaacaccag taccaagtac atggtttgat gaatacggtg ctttgggaat taatatgact
    26281 ttctatcgtc caaatgatgg aaatcagtgg tatattaccg atccgaagac tcttaaaaca
    26341 agagaagaca ttgataatta tatgaaaggc tatatagata ccttgtctct tcttgattat
    26401 gttgaaggtg atgcagtcat tagtaaacat gatgataaat taaatcaact gtggtttaag
    26461 aaagttgatc ctattgaacg cactgtaggg tcaaaagatc aatatgacaa ggttcgaaat
    26521 atgagctctg atgattggaa aggaataaca ttaaacagtg ttaatgactt agttgataat
    26581 aatctgatga ctaatcgtat gatgaataat aatatatata agccagaagt atttgatcca
    26641 ggtattgcct atgtaggaat taaaatgatg aatagtatct atgcaggtaa cacaagtgag
    26701 ggtgcacccg gtgctgttac atttaagaat aatgcattta gactctgggg atattatggc
    26761 tatgataaag gattcttagg atatgcttca gataaattaa aatctcaagc taataaagaa
    26821 gggaaacaat ttggagatca atatattata caaaaaatat ctgacggcag attccaatcg
    26881 tttgctgatt ttaagaaaga atattataaa gaattgttag aaaaagccaa cgctggtttt
    26941 actcctttta ctattgatgg tgagaccatt cataactatg atgaattaaa atcattattt
    27001 gccaaagctc ttgaagaaga tatggttact aataaagtta atggtaaact tgtgcaagta
    27061 aaagaaaaag tttataagaa acttcttcaa caaacaaata gctttaaaac ttcaatcttt
    27121 aaataattga aatctctcat ctgctttgcg gatgagggtt ttcttatttt atgctatact
    27181 taaggtatgc atagaaaaac agtgattgat tttaggattt tgggggagag atacaccttt
    27241 acccagccta tcaaagagat aaaaacgaga aatgtagcag aagtggcaga tttgctggca
    27301 caagtggaaa gctaccaaga gcaaggttat tatgtggtgg ggtatgtcag ctacgaggct
    27361 gcccctgctt ttgaggaaaa attagcagtt cacaaggatc ccctactggg agagtacctg
    27421 ctttacttta ctgttcatga tagggtagag acctccccta ttcctctgac ttatgaggat
    27481 attgatttgc cctcaaattg gcaggaagtg acgtctgcag cagactatga aaaggccatt
    27541 gcccagatac accatcactt gaggcaggga gacacctatc aggtcaacta caccgtccaa
    27601 ctcaagcaaa agttaagtgc caatcctttt gccatctaca atcgtatggt ggtagagcag
    27661 gaggcgggct acaatgccta tgtggaacat gacgagatgg cagtgatttc catgagccca
    27721 gagctctttt ttgagcaaaa tgatcgcgag ttgacaacac gaccaatgaa ggggacgact
    27781 cagcgtgggg taactgacca agaagatctt gaacaggcca gttggttgga acaggatccc
    27841 aaaaatcgct ctgaaaatat gatgattgtg gacctcttgc gcaatgatat gaaccgtatt
    27901 tctgaagttg ggagcgagca cgtggagcgt ctgtgccagg tagagcagta ttcaactgtt
    27961 tggcagatga cttcgaccat caagagtcag ttgcgagagg atgtggacct tgttgaaatc
    28021 ttccgctcac tctttccttg tggttccata acgggtgcac cgaaaattgc gacaatggag
    28081 attatcaagg acttggagcc tcaaccgcgt ggagtctact gtggaacgat tggtctcttg
    28141 cttccaaatg gacgacggat ttttaatgtg gccattcgta ccattcaact tcacaaaggt
    28201 caagccattt atggagttgg cggagggatt acttgggata gcacatggga atctgaatac
    28261 cgagaggttc atcaaaaggc agcagttctt tatcgtaaac aagcccgctt ccaactgatt
    28321 acgacagggg aaatcagcca gaagaacctg ctgtttgaag atcaacatct ggaaagactg
    28381 agaaaagcta gtcgttattt tgcctttcct tttgatgcag aagacttggg acacaagatt
    28441 gaggaagagt gtcaggattg tgaagctaat caagattacc gcttgcgaat cagccttagc
    28501 aaatctggag agatagaagt caatcgtcaa gtattaaccc ctctcagtac aagcttttgt
    28561 caggcccaag tctgccttca ggaagctgct ttgaatcaat cctttaccta ctttaaaacc
    28621 actcaccgac cgcatttgag cctaggagaa caagagaaga tttaccacaa taagtcagga
    28681 aaactgcttg aaacctctat aggaaatttg gttctgaaaa tcgctggaaa actctacaca
    28741 ccgcctatcc gacttggaat cttgccagga atttaccgtc agtatttgct agaaacagga
    28801 caggtagaag agaaagtctt gaccttggca gatttagccc aagcagaagc tatttacggc
    28861 tgtaatgcag tgagaggctt gtatgaacta agccttgagg agaactagat gaaactgata
    28921 tttttgcatg ggttagggca gtcagcagag tcttggaaag aagttcggaa tctactgaca
    28981 gattatcctt ctgaggctat cgagttattt ccttctggag ttagtaacta tcaacaagct
    29041 aaggagcgag tttatcagca cctagctcaa gagacagaac cttttgtttt gattggattg
    29101 tccttaggtg ctgccctggc actagaactt tccagttacg atttaccaaa tcttcgagct
    29161 ttgattctgt caggatgccc gcttaaatta gcagggaata tcctatttta tcttcagttg
    29221 ctgattttta aactacttcc caaaagagta tttgaaaaac agggagcaga caaggctctt
    29281 atggtcggag tttctgagga gttgaagaca cttgatttaa cggatatagc aggaacttgc
    29341 ccttatccaa ccttactaat ttgtggtatc aaagataaac cgaatcttag ttctatgaga
    29401 agtcttcata aactaatatc agaatcccaa tttcagatta tcccagatgg ccctcatgtc
    29461 ttgaataagg caaaaccaaa ggagtttgta gaaaagacca gaagtttcct tgaattgctg
    29521 aaataagttt gataaaataa tattgaaatc gatatcataa ttatgggaga aagaaatgaa
    29581 taaacgtcta tttttaaaaa tgagtctggt gacgttgcca attttagcct tgttttcaca
    29641 acctgtgtta gcagaggaaa acattcattt ttcgagttgt aaggaagctt gggctaatgg
    29701 atattcggat cttcatgagg gagaacctgg ttattctgcc aagttagacc gcgatcatga
    29761 tggtgtggct tgcgaattga aaaatgctcc taagggtgct tttaaagcaa aacaagcggc
    29821 tacaactcaa actgatacaa cttcatccac agcaagtggt tgggttaagc aggacggttc
    29881 ttggtactat tttgatggaa atggaaatct agtgaaaatg catggcaggg aaactattac
    29941 ctgaaagctg atggtaaaat ggcacagagt gaatggattt atgactcttc ttatcaagct
    30001 tggtattatt tgacatcaga tggttcttat gcttacagta catggcaagg agcttattac
    30061 cttaaatcaa acggtaaaat ggcacaaggt gagtgggttt atgattcttc ttaccaagca
    30121 tggtattact tgaaatcaga tggttcatat gctcgcaatg catggcaagg aaactactat
    30181 ttgaaatcag atggtaaaat ggctaaaggt gaatgggttt atgatgccac ctatcaagct
    30241 tggtattatt tgacatcaga tggttcttat gcttacagta catggcaagg aaattactat
    30301 ctaaaatcgg atggtaaaat ggctgtcaat gaatgggttg atggtggacg ttattatgtt
    30361 ggcgctgacg gagtttggaa ggaaggtcaa gcaagtacag cttcttctag taatgatagc
    30421 aatagtgaat attctgctgc tttaggaaag gcaaaaagtt ataattcgtt attccacatg
    30481 tcaaaaaaac gtatgtatag acaattaact tctgattttg ataaattttc aaatgatgca
    30541 gctcaatatg ccattgatca tttagatggt taacagatac tggtaggcga aaaaattcct
    30601 tataattcat ttacgcaaac ctttgcataa gtgaatggat tttgatatac tagtactgta
    30661 agcattttca attactacac aatgaaaaag gagaatatga tgagtcaaaa gattattggg
    30721 attgaccttg gtggaacttc tatcaaattt gcaatcttaa caacagcagg agaaatccaa
    30781 ggaaaatggt caatcaagac caacattttg gatgagggaa gtcatatcgt tgatgatatg
    30841 attgagtcta ttcaacatcg tttggacttg cttggattgg cagcagcgga cttccaaggt
    30901 attggaatgg gatcaccagg tgtggttgac cgtgaaaaag ggactgttat cggtgcctac
    30961 aacttgaact ggaaaaccct tcaaccaatc aaacaaaaga ttgaaaaagc tttgggcatt
    31021 ccatttttca tcgataatga tgccaacgta gcagctcttg gtgagcgctg gatgggtgct
    31081 ggagataacc aaccagacgt tgtctttatg acactcggta ctggtgttgg tggtggtatt
    31141 gtcgcagaag gcaaattgct tcatggtgtt gctggtgcag caggtgaact tggtcatatc
    31201 actgttgact ttgaccaacc aatctcatgt acttgtggta aaaaaggctg ccttgagaca
    31261 gttgcttcag caacagggat tgtcaacttg actcgtcgct atgccgatga atacgaaggc
    31321 gatgcagcct tgaaacgctt gattgataac ggagaagaag taactgctaa gactgtcttt
    31381 gatctcgcaa aagaaggaga cgaccttgct ttgattgttt accgtaactt ctcacgttac
    31441 ttgggaatcg cttgtgctaa cattggttca atcctaaacc catcaacaat cgtcatcggt
    31501 ggtggtgtgt cagctgcggg agaattcctt ctacaaggtg ttcaaaaagt ttacgatgaa
    31561 aatagtttcc cacaagtacg cacatctact aaattagctc ttgcaactct aggaaatgac
    31621 gctggagtta tcggagcagc atcacttgta ttgcaataag ataatgagag aggaaaatgc
    31681 taacctagag ttagtaatgt tcctctttta ttttttatgc tatactagtt aggacaatga
    31741 atgaggtgaa acgaaatgac aaaagcagat acgattttta aagagaatat tgaacgcatt
    31801 ctaaaggaag gtgttttctc agagcaggct cgtcctaagt acaaggatgg gactgttgct
    31861 aattccaagt atgtaactgg tgccttttcc gagtacgact tatctaaagg agaatttcct
    31921 atcacaacct tgcgtcccat tgcaatcaaa tccgccatca aagaagttct ctggatttac
    31981 caagaccagt caaatagctt agaagtgctt aatgacaagt acaatgttca ctactggaat
    32041 gactgggaag ttggagacac gggaaccatt ggtgagcgct atggtgccgt tgttaagaaa
    32101 cacgacatta tcaataagct tctcaaacag ttggaaacca atccttggaa ccgccgcaat
    32161 attatttcgc tctgggatta ccaagctttc gaagaaacag atgggctgct cccgtgcgcc
    32221 tttcagacca tgtttgatgt ccggcgtgtt gatggggaaa tctatctgga tgcgaccttg
    32281 acccagcgct ccaatgatat gctggtggcc caccacatca acgctatgca gtatgtggct
    32341 ttgcagatga tgattgccaa acattttggc tggaaggttg ggaagttctt ctacttcatc
    32401 aacaacctcc atatctatga taatcaattt gaacaagctc aggaattgct ccgtcgggag
    32461 ccgtcaaact gccaaccacg cttggtttta aatgttcctg atgggactaa tttctttgat
    32521 atcaaagcag aagattttga gttggtggat tatgaccctg ttaagccaca gttgaagttt
    32581 gacctagcta tttaaaagaa tagaaaaaag aagttgagaa taatcccaac ttcttttgtt
    32641 tcttaacgtg atacgcggcg acgagctgct tttttacggt tttcttcgat gaaagctgct
    32701 ttttgctctt ctggttcgat tactttcttt ttaaatgcgt atactgcacc tgcaacggca
    32761 gcgacagttc ctgcgacacc tgttacaaga cctttagcga atcctttagc catgagtctt
    32821 cctcctttat attctcaatc agccagcctc ctcaagaggt cacatttttc tgactgacct
    32881 ttttgtgtta taataatagt aacgaaaaaa tgggaatttt tcaaggaaaa aagatgaaaa
    32941 caaaaataat tgtgattgtt ggaccgactg ctgttggaaa gacggctcta gctattgaag
    33001 ttgcaaagtg ctttaatggc gaagtggtta gtggagatag ccaacaagtc tatcgaggac
    33061 ttgatattgg gacggccaag gctagtccag aagagcaggc agctgttcct catcatttaa
    33121 tcgatgttag agagataacc gagtcttact cggcttttga ttttgtttca gaagctaaga
    33181 tgactattga ggatattcac agccgtggca agctagccat tatcgccggt gggactggac
    33241 tttatatcca gagcttgcta gaaggttacc acctaggtgg ggagactcct catgaggaga
    33301 ttttagctta tcgagctagt ttggagccat attcagatga ggaattagcc catttggtgg
    33361 agcaagcagg ccttgagatt ccccagttta atcgtcgtcg tgctatgcgt gccttagaaa
    33421 ttgcccattt tggtcaggat ttggaaaatc aagagatttt gtatgaaccg ctgattatct
    33481 gcttggatga tgaacgtagt caactttatg agcgtatcaa ccaccgagtg gatttgatgt
    33541 ttgaggctgg gcttttggat gaggccaagt ggctgtttga ccattcccct aatgtacagg
    33601 ctgctaaagg aattggctat aaggaactct ttccttattt ccgtggagag cagacctttg
    33661 aggaagctcg tgagagtctt aaacaggcga cccgtcgttt tgccaagcgt cagctgacct
    33721 ggttccgtaa tcgcatgcag gtcacctttt atcagattgg agaatctggt gtacaagacc
    33781 gcattttaag ccagatagag gagtttttag atgattgaaa cggagaaaaa agaggagcga
    33841 gtcctgctga ttggtgtgga attgcagggt atggacagtt ttgacctctc catggaagaa
    33901 ttggctagtt tagcgaaaac ggcaggggca gtcgttgtag atagctacag acaaaaacgt
    33961 gaaaaatatg attccaagac cttcgtcggc tctggtaagt tggaagagat tgcgcttatg
    34021 gtggatgcag aagaaatcac tactgtcatc gtcaacaacc gtctgacccc aaggcagaat
    34081 gtcaatctag aggaagttct cggtgttaag gtcattgacc gtatgcagtt gattttggat
    34141 atctttgcca tgcgggctcg aagccatgaa gggaagctcc aagtccacct agcccaactc
    34201 aaatacctct tgcctcgctt ggttggtcag gggattatgc tcagccgtca ggcaggggga
    34261 attggttccc gtggtcctgg tgaaagccaa ctggagctga accgtcgtag cgttcgcaat
    34321 caaatcacgg atatcgagcg ccagctcaag gtggttgaga aaaatcgtgc gactgtcaga
    34381 gaaaaacgtt tggagtctag cacttttaag attggtttga ttggttatac taatgctggg
    34441 aaatcaacta tcatgaacat cttgaccagt aagacccagt atgaagcaga tgagctcttt
    34501 gcgactctgg atgcgacaac caagagtatt catctgggag gcaatctcca agtaactttg
    34561 acagataccg ttggctttat ccaagatttg ccgacagagt tggtgtccag tttcaagtca
    34621 accttggaag aaagcaagca tgtggacctt ctggttcatg ttatcgatgc tagcaatcct
    34681 taccacgagg agcatgaaaa aacggttctc tccatcatga aagacctgga catggaagat
    34741 attcctcact tgacgcttta taataaagcg gatttggtgg aggatttcac gcctacccaa
    34801 acgccatata ccctcatttc tgccaagtct gaggacagtc gtgaaaactt gcaagcatta
    34861 ttgctagata agattaagga aatttttgaa gcatttaccc tgcgagtgcc tttttcaaag
    34921 tcctacaaga ttcatgattt agagagtgtt gcaattctgg aagaacgtga ttatcaggaa
    34981 gacggcgaag tgattacagg ctacatttcc gagaaaaata aatggaggtt agaagaattt
    35041 tatgactgat attaaaactt tggctctaaa gtatggaggt tatacaagtc tggacaaggt
    35101 ctatctggat cagcttctag ctggtaaaac agagcaggag cagttaacac ttattacacc
    35161 tccgccaagt gttgttaatg cctactttgc agaactctat cagaaaaaaa gtcctcaggc
    35221 tgcgacggat tattttgcag aactcagtca ggaactgaat ctttacaata ctgagccaag
    35281 tttcacccta gaaaataagc cttttattcg gcttaatctg tctggtaaat cctttggttt
    35341 ttgttatgag agtgacggcc ttggtcggat tttctctgaa aatgaggaga aaatctcgga
    35401 tgacttgctc tttgaaattg cgcaaatttt ctcccatcaa ttagtctttg aagagtctgg
    35461 taagatttac atgaaacctg tcaaggacga ggaagttgtc agcctagaaa gtctcacagc
    35521 tttgactgat ttggaaagat tggctgatgg tcgaaaacgt ctcaaaggct acagccaaga
    35581 ggatttattg caagaagctg ctgctttttc tggcaaacgc tatttccgat cggaaaaccg
    35641 cacagccatg ttatatattg attaattaga aagtatcgaa tggatattca atttttagga
    35701 acgggggctg gtcagccctc taaagcccgc aacgtttcaa gtctcgccct gaaacttttg
    35761 gacgagatta acgaagtttg gctctttgac tgtggagaag gtacgcaaaa tcgcattctg
    35821 gaaaccacaa ttcgaccacg taaggtcagc aaaatcttta ttacccatct gcatggagac
    35881 cacatttttg gtttgccagg tttcctttct agccgtgcct ttcaggccaa tgaagagcag
    35941 acagatttgg aaatctacgg acctcaagga atcaagtcat ttgtcttaac cagccttcgt
    36001 gtgtcaggtt ctcgtctgcc ctaccgcatt catttccatg agtttgacca agattctctg
    36061 ggtaaaattc ttgaaaccga taaattcact gtgtatgcag aggagctgga ccacactatt
    36121 ttctgtgttg gctatcgtgt catgcaaaag gatctagaag ggacgctgga tgctgaaaaa
    36181 ctcaaggctg ctggtgttcc gttcggcccg ctttttggta aaatcaaaaa cggccaggat
    36241 cttgttttgg aagacggaac tgaaatcaag gcagcagact atatctcagc gccacgtcca
    36301 ggtaagatta tcactatttt aggagacact cgaaaaacgg atgccagtgt gcgtctggct
    36361 gtcaatgcag atgtcctagt tcatgagtcc acttatggca agggtgatga aaaaattgct
    36421 cgtaaccatg gtcactcaac taatatgcaa gctgcacaag tagcggtaga agcaggtgcc
    36481 aaacgcctcc tactcaacca tatcagtgcc cgtttcctct caaaagatat aagcaaactc
    36541 aagaaggacg ctgccacaat ttttgaaaat gtccatgtgg tcaaagactt ggaagaagtg
    36601 gaaatctagc agtcacagaa aggataagta tgcctactat tctcattacc ggagctagcg
    36661 gtggtctagc tcaagaaatg gtcaaactcc tgcccaatga ccaactcatc ttgcttggta
    36721 gaaataagga aaaattagcc caactctacg gaaattattc ccatgcagaa ttgattgaaa
    36781 ttgatattac cgacgattca gccctagaag ctctggtaac tgatctttat ctccgctatg
    36841 gcaagattga tgtcttgatt aacaacgctg gttacgggat ttttgaggga tttgaccaga
    36901 ttgctgataa agatattcac cagatgtttg aggtcaatac ctttgccctg atgaatctgt
    36961 ctcgtcacct tgcggctcgt atgaaggaaa gcagcaaagg gcatatcatc aacatcgtca
    37021 gcatggcagg tctaatagct actggcaagt ctagtcttta ctcagcgacc aagtttgcgg
    37081 ctattggttt ttcaaatgct ctgcgactcg aacttatgcc ctatggagtc tatgtgacaa
    37141 cagtcaatcc aggtccaatc cgaacaggat tttttgacca agctgaccca gatggaactt
    37201 atcttaaatc ggttgaccgc ttcctcttag aggcagatgc agtggctaaa aagattgtca
    37261 agattatagg caaaaataaa cgagaactca atctcccgat tttgttgaac ctagcccata
    37321 agttttatac tctctttccc aagctagctg ataagttggc aggggaaact tttaattata
    37381 agtaaaaaga accaatgtgc aggttgttgc tagcctacat attggttctt atcatttctc
    37441 aactatcaaa ctgaacttct tctagtagat agtcgataaa gcgttcgccc atcttagata
    37501 ggctggtttt ctcatgctgg atatagacca gctcaatcgg gtcatcaata tccagtggga
    37561 tagaaacgat attgtctccg tttaggttgc tgttcaaaat ccctgtcgca atggtataac
    37621 catccaaacc aatcaagaga ttaaagaggg tggcacggtc actgaccaca atggatttct
    37681 tgtggtgttc ctgagaaaga atctcttctg aaaagtagaa ggagttgtgc gtcccttggt
    37741 catagctgag gtaagggaaa ttctccaaat cagacagttt caccttgtct ttctttgcca
    37801 gagggttggt cttgctgaca aagatatgcg gttgcgctgt gaagagatgg tgggctagca
    37861 ggtgattgtc atccagcatc ttggttaaaa catcacggtt gtaactgttt aagaagagga
    37921 ccccgacctc actgcggaag ttcttgacgt cgtcgataat ctcccaagtc cgagtttcac
    37981 gaaggaagag ttcgtatttc tccatatcgc ttttcttgag caaagagaca aaggcattga
    38041 ccacaaaggc atagtgttga gacgaaacgc taaagagttc gcggtgggcg acaggatttt
    38101 tatagcgttc ctccagaagc tgggtctgct cgacaacctg acgggcataa gagagaaact
    38161 ccatcccatc acgggtcaag gtgattccct tgggattgcg gataaagatc tcaatgccca
    38221 tttcattttc caaatctcgc actgcattgg agagacttgg ctgagtgata aagagttgct
    38281 tggctgcctc attcatggag ccagtttcga cgattttgat aatataatgt aattgttgaa
    38341 ttctcatgtt tttattgtac catacttgcc ttagaatcgc taaagaagat aggaaaataa
    38401 ggagcattgt gtgagtctct tcttgaaaaa aacacaaaat tttgctagaa tgagtcttat
    38461 gaaaacattc tatgatgtgc agcaattcct caaacgattt ggtattattg tttacatggg
    38521 aaaacgctta tatgatattg aactgatgaa gttggaacta tctcggattt acgatgcagg
    38581 gttgatggat aaattagact atctagaggc agaagcagtt cttcgcagag agcacaaggt
    38641 agaattggat tatattgaga aaaatggaga aaagaactaa tggttacttg gattttgtgg
    38701 gcacttatac tagcaatgtt ggcgtggatg ggctttaact atcttcgtat tcgccgtgcg
    38761 gctaaaattg tggacaatga ggagtttgaa gccttgattc gtacgggtca attgattgat
    38821 ttgcgcgacc cagcagaatt ccacagaaaa catatccttg gtgcacgcaa tattccttca
    38881 agtcagttga aaactagtct tgcagccctt cgtaaagata aacctgtcct tctctacgaa
    38941 aaccaacgtg cgcaacgagt tacaaatgca gctctttact tgaaaaaaca aggtttttct
    39001 gagatttata tcctttctta tggcttggat tcttggaaag ggaaagtgaa gactagctaa
    39061 ccttatttcc aaattagtcg aatgatagaa tgaagctatt gttttatagt ataattaatt
    39121 tgtataaaat ttaaagagtt cttagaacat gaaagaaaag aagaaaaatc cactttttgt
    39181 gggtatcttg tcgattattc ttggtttgtt atttccaatt gtaggcctga ttttgggtat
    39241 cataggattg gtcttggcta tttcatacca aaaagagtca caattagact ataaaataga
    39301 aaagattctt aatatcttag gaattgtgat ttctgtagtt aactggattg tagcaatcgc
    39361 cttaattttt cggtaaataa aagaaaaaag ctttagtttt aaagcttttt ttgttttatc
    39421 gactcatctc taaatagttt ttaatgattt gtaactcttt ttggttcaaa gggtggtagt
    39481 taccttctgc taaatctggg ttcaatgtga agtccccaaa ttggattctc ttgaggttag
    39541 tcaccttaac accaaccgag aggaacattt tcttgatttg atgaaatttt ccttctgaaa
    39601 tggtgataga ggcttgactg agggaaggac ttgctgatag aatctctagt cttgcaggtt
    39661 tacagatagt gccatctaaa aagacaattc ctttttgaaa ggtttggata tggtcaggtg
    39721 ttagaagtcc attaacctca acttgataag tcttatcgac atgatattgg ggatggagga
    39781 gttggaagcc caagggaccg ttatcggtca agagaaggag tcctgttgta tctcggtcca
    39841 gtcggccgac ggcatagagc ttgtcagact ggatgtcaga tggaagcaag tccatgacgg
    39901 tcggaagttc cttgtctttg ttggctgtaa cgacaccagc aggtttatga agcataagat
    39961 aggtgtgttc atagccttga atgattcgat cctgaaaaag gagttcttgt agtcctgtat
    40021 cgatattttg agctagggag cgggctgggc aaccatcgac tagaatttcc cctctgagga
    40081 gtgcttgctt catggccttt cggctaactt tttcttgggc taataaatta tctaaacgca
    40141 tactgtgcct atcttatcat aagtcgcttg ctttgaaaag gcttgacgtg aaaagaaaag
    40201 cggagtgaaa acatttacac ttgcttgagt tatgttattt atttgaaatt atggtataat
    40261 cgttcagtta gaaaataaat tttgaatatt atagaggaaa tcatgacaaa attaagagaa
    40321 gatatccgta acattgcgat tatcgcccac gttgaccacg gtaaaacaac cctggttgac
    40381 gaattattga aacaatcaga aacgcttgat gcacgtactg aattggcaga gcgtgctatg
    40441 gactcaaacg atatcgaaaa agagcgtggg attaccatcc ttgctaaaaa cactgccgtt
    40501 gcttacaacg gaactcgtat caacattatg gacacaccag gacacgcgga ctttggtgga
    40561 gaagttgagc gtatcatgaa aatggttgac ggtgttgtct tggtcgtaga tgcctatgaa
    40621 ggaaccatgc cacaaactcg tttcgtattg aaaaaagcct tggaacaaga ccttgtccca
    40681 atcgtggttg ttaacaaaat cgataagcca tcagctcgtc cagcagaagt agtggatgaa
    40741 gtcttggaac ttttcatcga gcttggtgca gatgacgacc agcttgattt cccagtggtt
    40801 tatgcttcag cgatcaacgg aacttcttca ttgtcagatg atccagctga ccaagaagcg
    40861 actatggcac caatctttga cacgattatc gaccatatcc cagctccagt agataactca
    40921 gatgagcctt tgcagttcca agtgtcactt ttggactaca atgacttcgt tggacgtatc
    40981 ggtatcggtc gtgtcttccg tggtacagtt aaggttgggg accaagttac cctttctaaa
    41041 cttgacggta caactaaaaa cttccgtgtt acaaaactct tcggtttctt tggtttggaa
    41101 cgtcgtgaaa tccaagaagc caaagcgggt gacttgattg ccgtttcagg tatggaagac
    41161 atctttgtcg gtgaaaccat cactccgaca gatgcagtag aagctcttcc aatcctacac
    41221 atcgatgagc caactcttca aatgactttc ttggtcaaca actcaccatt tgctggtaaa
    41281 gaaggtaaat gggtaacttc tcgtaaggtg gaagaacgct tgcaggcaga attgcaaaca
    41341 gacgtttccc ttcgtgttga cccaactgat tcaccagata aatggactgt ttcaggacgt
    41401 ggagaattgc acttgtcaat ccttatcgaa acaatgcgtc gtgagggcta tgaacttcaa
    41461 gtatctcgtc cagaagttat cgtaaaagaa atcgacggtg ttaaatgtga accatttgaa
    41521 cgtgtacaaa tcgacactcc agaagaatac caagggtctg ttatccaaag cctttctgaa
    41581 cgtaaaggtg aaatgttgga tatgatttca actggtaatg gtcaaactcg tttggtcttc
    41641 cttgttccag cgcgtggttt gattggatac tcaactgagt tcttgtcaat gactcgtggt
    41701 tacggtatca tgaaccatac cttcgaccaa tacttgccat tgattccagg ggaaattggt
    41761 ggacgtcacc gtggtgccct tgtttctatc gatgctggta aggctacaac ttactcaatc
    41821 atgtctatcg aagaacgtgg tacgatcttt gtcaacccag gtactgaggt ttatgaagga
    41881 atgatcatcg gtgaaaactc tcgtgaaaat gacttgacag ttaacatcac gaaggcaaaa
    41941 caaatgacca acgttcgttc agctactaag gaccaaacag ctgttatcaa gacgcctcgt
    42001 atcttgacac ttgaagagtc tcttgagttc ttgaacgacg atgagtacat ggaagtaacg
    42061 cctgagtcta tccgtttgcg taaacaaatc cttaacaaag cagagcgtga gaaagctaac
    42121 aagaagaaaa aatcagctga ataagagcta gaaagagaga aagatggtct atttagtcct
    42181 aggaatttta ctgctcctac tctatgtatt tgcgacacca gaaagcatta aagggacagt
    42241 caatatcgtc gctatggtat gtattttagt ggcactcttg attttattgg ttctatcttt
    42301 tctgaaaatt tttcaattac caacagaaat attcctagca atagccatgt tgatcctagc
    42361 ttactttagt gttagagaca ttacactcat gccagtcaaa aaaagtaaaa gaagataaaa
    42421 aagagagttt tggctctctt ttttgctagg aattttacaa tagtttgaat tgttttgtac
    42481 tataacatca tctcttttgc ttggatagta tgaatgggaa aaacttgaat aaaattcgtt
    42541 aatggcatct attttatatt tggttagtac tggttgcata ttattttaaa ataaaactac
    42601 tatataatat tattcataga ggcaatgtag taaaatatct aaaaaattcg attttttcct
    42661 tgacaacaaa taataaaaat gatattttta tcacataaga aagcggtttc aacaaaagag
    42721 agaggagggt ttattttgtt gcattgttgc tcaaggtgtg ctttttttga aagaaaaaat
    42781 aaaatgaaaa cgaaaaaaca tagattactt gctctagctc ttatttcaag ttttacatta
    42841 ttgggagctg catcagctgc tgtacaatat ccagatggag gagtatggac gtatggagaa
    42901 ggttcatgag gtggttgggc tttttcaaat tactatcatg gtaaaaaata tcattattct
    42961 tctcttgtaa gtagatggaa tagtcattca gataaaggag aagcttctgc tggaaaaacc
    43021 tcctatgcat ggatttggac taaatgggga gaacaagtag cattttactg tgattatgac
    43081 taatcgttca ttgatttaaa tcgttcccct tgtaagaaat tacaggggga attttgttta
    43141 caaggagtct actatgaaga aactattcat attattatca actttttttc tcagcttctt
    43201 ccttgcttgg attattgtct tacgtgcgcc acaatattta tatgcaagct atgattccgt
    43261 ttctttactt cgtgtcaaaa aagatactca ggaaccgacg cgtgaggtat ttgaacagga
    43321 attggagaat tttgcaaact cagaacagag tttaatagct agaagaattg tagagccgag
    43381 taaggatggg acgactcact ttacttatgc aacttatggt cagggaactt taccaaaaga
    43441 attccaagaa gctagccaag aaagtcgtga acgtagtgat ccgctaaata gttatctcct
    43501 tttgtcaggc tccttgacga aagaaaagct tgccgataaa ttaggagatt tgggttataa
    43561 agcaagtgct gaccgaaaga taccgcccta ttttcttgct tttcgaatat tactaaatcc
    43621 ccttatttta attagtttag caatatttgg cttatctttc tttgctttag tgattatcac
    43681 tcggattaag gaaatgagag cagcaggtat aaaactcttt tctggtcaga ctctcttatc
    43741 catcatgggg cattctttat ctactgatat caaatggctc cttctatcag ccctcctttc
    43801 cttcctaggt gggggagtcg ttctttttag tcaaggtttg ttttatccta tcttgttagc
    43861 cacctatggt tttgggatta gtttctatct gttgttttta ttggcgattt caattttact
    43921 aatgcttctt tatctaatga gtttgagtta caaagcatta gttcccgtta ttaaggggag
    43981 attacccctt aaacgcctga tgattttaac cctattgtgt cagttggttg ctgtctttac
    44041 agtaggctac gctgttaaga caggtttgat gtcttaccaa cgattgaaag aacttgaaat
    44101 ttcaaaacaa gcatggcagg atagagcaga ctattatcaa atttcttttg gcttaggtga
    44161 tagaggaaaa gatacagaaa atcagagcaa gtggtatgcc tttgccaagg aagcaatcga
    44221 agaagaacaa gctctttatg taaaggataa tctgctccat tttgccaatc cacaaggaaa
    44281 aaatgaacag ggagagacac tggataccta tagtccagat gctaatacgc tctatgttag
    44341 tcccagttat ttggacaagg aaaaggtcgt ggtagatgct gagaccaaac agaagttagc
    44401 ccatctccaa aaaggtgagt ttatcctctt gctcccagaa catttgcgct ctcgagaagc
    44461 agaacttaag aaagtttttg aagaaagatt gagttattat ggaaaatctg gtgaggaggc
    44521 aagtgctcct ttggattatg agatgaaagc gcacgttagt tatctttcaa tgggagaaaa
    44581 gcggtttgtt tataataacg gtgagaatcc cgtatctact cagtatttga ctgatccgat
    44641 tttagtggta ttcacgccga cttctacagg tgatagtttt atatccttat ctagttggtc
    44701 tatcaatgct ggaaaacaac tctttatcaa aggatatgag agtgggctag aactcttgaa
    44761 gaaagctgga atttatgagc aagtatccta tcttaaagaa ggaagaagtg tttatctaac
    44821 tcgttataat gaagttcaaa ctgaaacagc aactttaatc ttaggagcta ttgtggggat
    44881 agctagttcc ttgttactat tttattctgt caatcttcta tatttcgagc aattccgccg
    44941 agatattttg attaaacgaa tttcaggttt acgatttttt gaaacacatg ctcagtatat
    45001 ggttagtcaa tttgccagtt ttgtatttgg tgctagtctc tttattttaa gcagtcgaga
    45061 cttggtgatt ggcttgctca ctttattagt ctttctagct agtgcagttc tgacgcttta
    45121 ccgtcaagcg cagaaagaat ctcgtgtttc tatgacaatt atgaaaggaa aataggatga
    45181 ttgaactaaa gaatatatct aaaaaatttg gaagccgtca gctattttca gatacaaatc
    45241 ttcattttga aggtgggaaa atttatgcct taatcggtac aagtggctgt ggtaagacaa
    45301 cattcttgaa tatgattgga cgattagagc catatgacaa aggacaaatc atctatgatg
    45361 gtacttctct taaggacatc aagccttctg ttttctttag agattacttg ggatacttat
    45421 ttcaagattt tggcttaatt gaaagccaaa ccgtcaaaga gaatctcaat ctgggtttag
    45481 ttggtaaaaa gttgaaggaa aaagagaaaa tctctttgat gaaacaagct ctaaactgtg
    45541 taaacctctc ttatttggat ttgaagcaac ctatatttga gttatcagga ggagaagcac
    45601 aacgtgttgc attagcgaag ataattttaa aggatccacc tttgatcctc gcagatgaac
    45661 caaccacttc cttagacccc aaaaactctg aggaattact ttccatccta gaatctttaa
    45721 aaaatccgaa tcggaccatt attattgcga cccacaatcc tctgatttgg gaacaagtgg
    45781 atcaggtcat tcgagttacc gatttatcac atagatgata tggtaagatt cagttagaag
    45841 aaagagtcac aaacacactt tgtggctttt ttatttccat aaaaatggta aaatagtagg
    45901 agtagaaatg gagttcgaga catgaaagta atagatcaat ttaaaaataa gaaagtcctt
    45961 gttttaggtt tggccaagtc tggtgaatct gcagctcgtt tgttggacaa gctaggtgcc
    46021 attgtgacag taaatgatgg gaaacctttc gaggacaatc cagctgccca aagtttgctg
    46081 gaagaaggga tcaaggtcat tacaggtggc catcctttgg aactcttgga tgaagagttt
    46141 gcccttatgg tgaaaaatcc aggtatcccc tacaacaatc ccatgattga aaaggctttg
    46201 gccaagggaa ttccagtctt gactgaggtg gaattggctt atttgatttc agaagcaccg
    46261 attattggta tcacaggatc gaacggtaag acaaccacaa cgactatgat tggggaagtt
    46321 ttgactgctg ctgggcaaca tggtctttta tcagggaata tcggctatcc agctagtcag
    46381 gttgctcaaa tagcatcaga taaggacacg cttgttatgg aactttcttc tttccaactc
    46441 atgggtgttc aagaattcca tccagagatt gcggttatta ccaacctcat gccaactcat
    46501 atcgactacc atgggtcatt ttcggaatat gtagcagcca agtggaatat ccagaacaag
    46561 atgacagcag ctgatttcct tgtcttgaac tttaatcaag acttggcaaa agacttgact
    46621 tccaagacag aagccactgt tgtaccattt tcaacacttg aaaaggttga tggagcttat
    46681 ctggaagatg gtcaactcta cttccgtggt gaagtagtca tggcagcgaa tgaaatcggt
    46741 gttccaggta gccacaatgt ggaaaatgcc cttgcgacta ttgctgtagc caagcttcgt
    46801 ggtgtggaca atcaaaccat caaggaaact ctttcagcct tcggtggtgt caaacaccgt
    46861 ctccagtttg tggatgacat caagggtgtt aaattctata acgacagtaa atcaactaat
    46921 atcttggcta ctcaaaaagc cttgtcagga tttgataaca gcaaggtcgt cttgattgca
    46981 ggtggtttgg accgtggcaa tgagtttgac gaattggtgc cagacattac tggactcaag
    47041 aagatggtca tcctgggtca atctgcagaa cgtgtcaaac gggcagcaga caaggctggt
    47101 gtcgcttatg tggaggcgac agatattgca gatgcgaccc gcaaggccta tgagcttgcg
    47161 actcaaggag atgtggttct tcttagtcct gccaatgcca gctgggatat gtatgctaac
    47221 tttgaagtac gtggcgacct ctttatcgac acagtagcgg agttaaaaga ataatatgaa
    47281 aaaaattgtc tttacaggtg gggggacggt tggacacgtt accctcaatc ttttgttaat
    47341 gcccaagttc atcgaagatg gttgggaagt ccactatatc ggggacaagc gtggtatcga
    47401 acaccaagaa atccttaagt caggtttgga tgtcaccttc cattctattg cgactggaaa
    47461 attgcgtcgc tatttctctt ggcaaaatat gctggacgtc ttcaaagttt gctggggaat
    47521 cgtccaatcg ctctttatca tgttgcgact tcgtccacag accctttttt caaagggggg
    47581 ctttgtctca gtaccgcctg ttatcgctgc gcgtgtgtca ggagtgcctg tctttattca
    47641 cgaatctgac ctgtctatgg gcttggccaa taaaatcgcc tataaatttg cgactaagat
    47701 gtattcaacc tttgagcaag cttcgagttt gtctaaggtt gagcatgtgg gagcagtgac
    47761 caaggtttca gatcaaaaaa atccagaacc agatgaattg gtggatattc aaagccactt
    47821 taatcataaa ttgccgactg tattgtttgt tggcggttct gcaggtgctc gtgtctttaa
    47881 ccaattggtg acagaccata agaaagaact aacagagcgc tacaatatta tcaatctaac
    47941 tggagattct agcctgaatg agttgagcca aaatcttttt cgtgttgact atgtgaccga
    48001 tctctatcaa cccttgatgg aattggctga tattgttgtg acacgaggtg gtgccaatac
    48061 gatttttgag ctcttggcga tagcaaaatt gcatgtcatt gtgccgcttg gtcgtgaagc
    48121 tagtcgtggt gaccagcttg aaaatgcagc ttactttgta aaaaaaggct atgcagaaga
    48181 ccttcaagaa agcgatttga ccttggatag tttggaagag aagctttctc acttactaag
    48241 tcacaaggaa gattaccaag ctaagatgaa ggcttctaag gaattgaaat ctctagcaga
    48301 tttttatcaa ttgttgaaaa aagatttatc ataaggaaag taaatgtcaa aagataagaa
    48361 aaatgaggac aaagaaatcc tcgaagaatt gaaagagtta tcagaatggc agaaacgaaa
    48421 ccaagaatat ctaaaaaaga aggctgaaga agaggcggct ctagctgagg agaaggaaaa
    48481 ggaaagacaa gctcgaatgg gagaagaatc tgagaagtca gaggacaaac aggaccagga
    48541 gagtgaaaca gaccaggaag attcagaatc agctaaggaa gagtctgaag aaaaagtagc
    48601 atcctcagag gctgacaaag agaaagaaga gaaagaagaa ccagagtcta aagagaagga
    48661 ggaacaggat aaaaagcttt ctaaaaaggc tacaaaggaa aaaccagcca aagcaaagat
    48721 tcctggtatc catatcttgc gagccttcac gattttattt ccaagtctgc ttttattgat
    48781 tgtctctgcc tacttgctca gtccttatgc gaccatgaaa gatattcgtg ttgagggaac
    48841 ggtgcaaact acagctgatg atattcgaca ggcttcaggc attcaggatt cggattatac
    48901 gattaacctt ctgctagaca aggcaaaata tgaaaagcag attaagtcta actattgggt
    48961 tgaatcagct caacttgtct atcaatttcc aactaagttc actattaagg tcaaggaata
    49021 tgatattgtg gcctactata tttctggtga aaatcattat cctattcttt ccagtggtca
    49081 gcttgagact agttctgtga gtctgaacag tttaccagaa acttatttat cagttctctt
    49141 taatgatagt gaacaaatca aggtttttgt ctcagaactt gctcaaatta gcccagaact
    49201 caaggcggct atccaaaagg tggaattagc cccaagcaag gtgacatccg atttaattcg
    49261 attgaccatg aatgattcgg acgaagtctt ggttcctcta tctgaaatga gtaagaaact
    49321 gccatattac agtaagatta agccacaatt gtcagaaccg agtgtggtcg acatggaagc
    49381 tggaatttac agttacactg tggcggataa attaattatg gaggctgagg aaaaagccaa
    49441 acaagaggcc aaggaagccg agaaaaaaca ggaagaagaa cagaaaaaac aagaggaaga
    49501 gagcaatcga aatcaaacga cccagcgctc atcgcgtcgc taggtttacc ttttctctta
    49561 tagttcttta gtgaccatgt ttttacttga caagtgctag tagaaataat agaataatag
    49621 aaaaccttta aagcagtcca gagaggcagc taaggttaga cggtgaaagg gtggagacta
    49681 cccatttttc gtggaacctt gctgttggca ggttcctttt ttcgtggctt ctgttggcca
    49741 gactctctca ctagtaaagg taaaaggaga aacctatgcg agaacatcgt ccaatcattg
    49801 ctcttgattt tcctagtttt gaggcggtca aggaattttt agctcttttc ccagcagaag
    49861 aaagccttta tctcaaggta gggatggagc tttattacgc agcggggcct gagattgtgt
    49921 cctacttaaa aggtttgggt catagtgtct ttttggatct caaacttcat gacattccta
    49981 atacagtcaa gtcagccatg aaggtcttgt ctcagcttgg tgtcgatatg actaatgtcc
    50041 atgcggctgg tggtgtagag atgatgaagg cggcgcgtga aggtcttggg agtcaagcca
    50101 aattgatcgc tgtaacgcag ctcacatcaa cgtcagaagc tcagatgcag gagtttcaaa
    50161 atatccaaac cagtctgcaa gagtctgtga ttcactatgc caagaagaca gctgaagctg
    50221 gcttggatgg tgttgtttgc tcggctcagg aagtacaagt catcaagcag gctaccaatc
    50281 cagattttat ctgtctgaca ccagggattc gtccagctgg tgttgcagtt ggagatcaaa
    50341 aacgagtcat gacacctgct gatgcctatc aaatcggcag tgactatatc gtagtgggac
    50401 gtcccattac ccaagctgag gatcctgttg cagcttatca tgccatcaag gatgaatgga
    50461 cacaggactg gaattaaaga actagattag aaaaataaaa ggagaatacc atgacacttg
    50521 ctaaagatat cgctagccac ctcttgaaaa tccaagccgt ttacctcaaa ccagaggaac
    50581 ccttcacttg ggcatctggt atcaagtcac cgatttacac tgataatcgt gtgacactag
    50641 cctatccaga aactcgtacc ctaattgaaa atggttttgt ggaagctatc aaagaagcct
    50701 ttcctgaagt agaagtgatt gcaggaactg caacagcagg gattccacac ggagccatta
    50761 ttgctgataa gatggacttg ccttttgcct acatccgtag caaaccaaaa gaccacggag
    50821 ctggtaatca aatcgaaggt cgcgtagctc aaggtcaaaa aatggtagtg gttgaagacc
    50881 ttatttcaac gggtggttca gttcttgaag ctgtagcagc agccaagcga gaaggagcag
    50941 atgtacttgg agttgtagcg attttcagct accaattgcc aaaagcagat aagaactttg
    51001 cagatgctgg tgttaaactt gtgacgcttt caaactatag cgagcttatc catctagccc
    51061 aagaagaagg ttacatcacg ccagagggcc ttgatcttct aaaacgcttt aaagaagacc
    51121 aagaaaattg gcaagaaggt taggtcagta agataaagag agacgaggct accgagtctc
    51181 ttttaccatt ttatttaaaa tatgacaata ttttaaataa aataattgac aacgctttct
    51241 tttattgata taatgaaaat gtaatatgta tcaaattgat caatcaacta tcttgtttat
    51301 cacttcttaa gaggacgatg tcatgaaaag aagaaaaata tattcctttt ttgcgtagtt
    51361 acaattgcat ctagtttgtt tctccctaca gttttagcta gacagattgg aaattatgat
    51421 ttaacgttgc cgcgttgggg ttcggataca actagtgagc ttgagaaaga aaacgcctct
    51481 gctggaatta ataatagtga cagcactggt ggcggtaaaa ggttaaatac ctctattcgt
    51541 agcgcctata gtgggtcaga tattaccccg gtatattcat tggggtctgg ctctaggatt
    51601 gtcatgtact ataatggagg tggtgacaat tatattggtt ctggtactag attagctatg
    51661 gcgccacaat ttggaaatta tgtaagaatt catacttcag gttcttggag tccagattct
    51721 tattaactta cttgtcagag taagccttaa agatggttga ttgtgggtgt agcatgaaaa
    51781 aagaatgcta caccctattt ttattataag gaggagtaag gatggaattt ttcatttgta
    51841 atcttgtacg agtcgttcaa tcacctcggt tttatatgtc tttatttttg acccttcttt
    51901 gcatgagttt aggaaatttc cttgctttca atggtattta taaaattgaa ggtttatcga
    51961 ttttttttgc cgcttcttct attcgaggat tttcaccgat tagcctagta gctgcactta
    52021 tctgtacact gccctattct agtcagataa tagaggatgc tgagagtcat tttctaacag
    52081 cacaattgtg ccgaatttct aaaaagaggt atctagctat tgtaggtagt actgcaatta
    52141 tttcttcttt tctagtcttt tttctcccct atttattatt attaggaatt aatcttttag
    52201 tgactcctta tcaggaaatt tatattggag attatagtgg tgccttaaaa gaattatttg
    52261 attccaatca gtttctctat agtcttgtga cgactctctg gtatggagtt tggggcgctg
    52321 tgttctctat ttttggacta gctagtgctt tgctagtgaa gaaaaaatta ggagctattt
    52381 tcatcccagt tgcctatatg atggttggtg gtattttatg ggctatttta gggctatctt
    52441 acttagaacc tgtgacaacg ctagctttgg gatatcagaa agatatcagt ctttccttag
    52501 ttagtgggca tcttgctttt attttatttg ttagttgttt ggttgtttat ggtacatttt
    52561 ttctacattc agaggactat gtataatgaa acaatttgtt caattttata aaaaagattt
    52621 cttagcagta ttggtttatt ttatattact gctatcctgt gttttatcta gtacagtata
    52681 tttattgcgc tgtcgccaat attcaatcca tccaaatgta ttagaatgga tcttagtttt
    52741 acttcaagat atgacgactg gagtatattg ctttccgttc acatatatat tattcttttt
    52801 ttatttgatg aataactatt ttaataggtt ggagtgtcgt attcgtctga aatcaattaa
    52861 gcactttacc agttttagtt tcaaattagc agctcttagt acggggattt ggacggcgac
    52921 tttattttta ttgatttttc tgattgcatt tagtaatggt tttagcttct ctttggagat
    52981 aaaggaggtt gattttttaa gagaatttta tggtataagt attgcaaaca atgctagttt
    53041 ttttatagga ttttttttct cttatatagc atactatttc tttttatcct tacttactat
    53101 tagcagtttt tcttggttta aaaaatcaaa catgagctta gtatttctgt ttactttttt
    53161 atttgtagaa tccttattct ggatttatca gttggacaat gggataattg gattattgcc
    53221 aatttttcag tatatggtaa attccaatcc gtatgcattg atttattggc ttacattact
    53281 atctatcata attccattga ctgtattttc tgttcataga aactggagga gagtgtaaaa
    53341 gttggaaatg ggaaagttaa gtagtcacat gtggaggttg aatcagataa tctataccaa
    53401 gtacttttgg ggttatgttc ttttttggat attgatttgt ttaggattat ggtattggtt
    53461 agaaggaaat gatagacttg ttatagaaat tttaaaaggg cctaatctga gtcaaaactc
    53521 ttttttagtc ttatctatat ggttgcttca ttggtttatt attcatacat tttttctagc
    53581 agttgtatat cgtagaagag catccgattt ctttatggaa gtgattcgat tttcttctat
    53641 taagctctgg attaggtatc agatttggac ctgttttctt tatggactca ttttaatcat
    53701 ggtaaaagtt ctagtgattc aatttatgtt acagttacca aactgggata taggagtttt
    53761 gtttatagtt gattctttga atgcttgtgt gttagtcttg ttttgcttta tgttatacgc
    53821 actaggagcg aatgtacaaa tgaactttgc ttgcgttagt ttctttttac tcatgattgt
    53881 gtttggtggt ctatttgtag gaaatcgaac taattatcta ttttatattt tgaatcgagg
    53941 gaatggagat ataggacgag atttatttct tcagttgcta tttttagtct ttctttttaa
    54001 aagtattttc tatttcactc gtcaaaagag gaggtttata gaatgaatga aataatcata
    54061 ttaaaaaata ttgagttgaa attaaaaaaa acatgtgttt ttcaaaacct taattttagt
    54121 tgtaaacagg gggaaattat aggaattact ggtgcgaatg gctcagggaa aagtgtattg
    54181 tttaaattaa tagctggttt atatagtccg tcttatggag aagtgttaat caatggggaa
    54241 aatattgttc ctgagagaaa aattccagct aatttgggag ctttgattga agaacctggt
    54301 tttataaatt attatagtgg ctttaagaat ttacaatatt tggcaagcat acgaggagta
    54361 gttggtaatc aggaaatcaa tgatacactg aaaatagttg gtctatatga gcaaaaagac
    54421 cagaaagtta aaacttattc gctaggtatg aggaaaaagc tagggattgc tcaagcaatt
    54481 atggagaatc cctctattct tttactagat gaacctatga atgccttgga taaatcaagt
    54541 gtagaaaata tgagaacatt gtttagaaag ctctctagtg aaaaagggac aacaattttg
    54601 attgctagtc atagtgaaga ggatattcgt atcttatgtg ataaagtata tgcaatagaa
    54661 gataaagtat gtacactgtg ttcagattga acatcttaga tagactcagg atgttatagg
    54721 taaaatctta aaaagggatt ggatcttaaa atccagttcc ttttaagagt taatgactaa
    54781 tcaacttttc caccttcaac aaccaggtca tctgctttag cagcgtcacc gtaggttagg
    54841 tgaagtgttt tttcataggc cttgtggaag aagttttcct tgcctaattt ttcaatatct
    54901 ttattgatga agtctagcaa ttcttggttg cctttttgaa ctgctgccgc aatggtatcg
    54961 ggatcaccga gggaagtaat tcctacttca aatcctttat tttcaagcgc ccaagctaga
    55021 acttccgtat tgtcagttga aaaggcatct ccacgtccgt caagaagagc ttggtaagag
    55081 tcactgtatt ggtcgtattt ttggagtttg atttctggat gattcttttc aaaataagtc
    55141 tcagcagtcg ttccttttgt gacaattaag gttttacctt caagttgttt gacgtctgta
    55201 atgagaccag tcttaggtga tacgacaccc agagaaactt tcatatatgg aagggcaaaa
    55261 tcaacttgtt tcttacgttc gtcagttact gtaaagttag caagagtaat atctaccttg
    55321 tttgaaatca agtattccgc acggttggca gcatcgactg aaatgtattt aaccttgaca
    55381 ccaaggtctt gagctagttg gttccctagt tcaatatcgt agccttggta agaaccatca
    55441 ttgtcaacgt agccaaacgg ttttttatct ccaaacacgg cgattcgcag ttcaccgctt
    55501 tttttgattt catcgatagt gcgagctttg gcagttgttt ttccagagga tgaaccagcg
    55561 tttccacctg agctacaagc agtgataaag ataagggcaa aggcaagtgc taaaacagtt
    55621 aagagtggtt tgaatagttt cataagaatc tccttta
//
LOCUS       DAVBDG010000006        50394 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBDG010000006 DAVBDG010000000
VERSION     DAVBDG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.006"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(46..1935)
                     /locus_tag="VQ526_000319"
     CDS             complement(46..1935)
                     /locus_tag="VQ526_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2554091.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFRERTVEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVKEKDQSKQLLTWIFPSILSISLLLSICFYFIYRRKTAPSIK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2073..3638
                     /locus_tag="VQ526_000320"
     CDS             2073..3638
                     /locus_tag="VQ526_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2554092.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYN
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKVIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3638..4378
                     /locus_tag="VQ526_000321"
     CDS             3638..4378
                     /locus_tag="VQ526_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2554093.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4487..4702)
                     /locus_tag="VQ526_000322"
     CDS             complement(4487..4702)
                     /locus_tag="VQ526_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554094.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5573..6823)
                     /gene="ilvA"
                     /locus_tag="VQ526_000323"
     CDS             complement(5573..6823)
                     /gene="ilvA"
                     /locus_tag="VQ526_000323"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2554095.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6873..7229)
                     /locus_tag="VQ526_000324"
     CDS             complement(6873..7229)
                     /locus_tag="VQ526_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2554096.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7467..7703)
                     /locus_tag="VQ526_000325"
     CDS             complement(7467..7703)
                     /locus_tag="VQ526_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554097.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERADATKSARYIAKWILRNKKH"
     gene            complement(7810..8832)
                     /gene="ilvC"
                     /locus_tag="VQ526_000326"
     CDS             complement(7810..8832)
                     /gene="ilvC"
                     /locus_tag="VQ526_000326"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2554098.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8898..9374)
                     /gene="ilvN"
                     /locus_tag="VQ526_000327"
     CDS             complement(8898..9374)
                     /gene="ilvN"
                     /locus_tag="VQ526_000327"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2554099.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9367..11067)
                     /locus_tag="VQ526_000328"
     CDS             complement(9367..11067)
                     /locus_tag="VQ526_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2554100.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVKFHA
                     "
     gene            complement(11268..12935)
                     /locus_tag="VQ526_000329"
     CDS             complement(11268..12935)
                     /locus_tag="VQ526_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2554101.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(12938..13303)
                     /locus_tag="VQ526_000330"
     CDS             complement(12938..13303)
                     /locus_tag="VQ526_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2554102.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13460..13648)
                     /locus_tag="VQ526_000331"
     CDS             complement(13460..13648)
                     /locus_tag="VQ526_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2554103.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13764..14249)
                     /locus_tag="VQ526_000332"
     CDS             complement(13764..14249)
                     /locus_tag="VQ526_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET2554104.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
                     I"
     gene            complement(14518..16062)
                     /locus_tag="VQ526_000333"
     CDS             complement(14518..16062)
                     /locus_tag="VQ526_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2554105.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIRDAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMESEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16275..16577
                     /gene="gatC"
                     /locus_tag="VQ526_000334"
     CDS             16275..16577
                     /gene="gatC"
                     /locus_tag="VQ526_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2554106.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16577..18043
                     /gene="gatA"
                     /locus_tag="VQ526_000335"
     CDS             16577..18043
                     /gene="gatA"
                     /locus_tag="VQ526_000335"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2554107.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18043..19485
                     /gene="gatB"
                     /locus_tag="VQ526_000336"
     CDS             18043..19485
                     /gene="gatB"
                     /locus_tag="VQ526_000336"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2554108.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEIQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19609..20652
                     /locus_tag="VQ526_000337"
     CDS             19609..20652
                     /locus_tag="VQ526_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HET2554109.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEGFIELT
                     QDKSQIKILVEPE"
     gene            21030..21590
                     /gene="efp"
                     /locus_tag="VQ526_000338"
     CDS             21030..21590
                     /gene="efp"
                     /locus_tag="VQ526_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2554110.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            21612..22001
                     /locus_tag="VQ526_000339"
     CDS             21612..22001
                     /locus_tag="VQ526_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2554111.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            21994..22416
                     /gene="nusB"
                     /locus_tag="VQ526_000340"
     CDS             21994..22416
                     /gene="nusB"
                     /locus_tag="VQ526_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2554112.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(22466..22702)
                     /locus_tag="VQ526_000341"
     CDS             complement(22466..22702)
                     /locus_tag="VQ526_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2554113.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(22715..23188)
                     /locus_tag="VQ526_000342"
     CDS             complement(22715..23188)
                     /locus_tag="VQ526_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554114.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(23206..23391)
                     /gene="briC"
                     /locus_tag="VQ526_000343"
     CDS             complement(23206..23391)
                     /gene="briC"
                     /locus_tag="VQ526_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2554115.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(23550..24317)
                     /locus_tag="VQ526_000344"
     CDS             complement(23550..24317)
                     /locus_tag="VQ526_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2554116.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(24314..25180)
                     /locus_tag="VQ526_000345"
     CDS             complement(24314..25180)
                     /locus_tag="VQ526_000345"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2554117.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNVGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(25217..26584)
                     /gene="accC"
                     /locus_tag="VQ526_000346"
     CDS             complement(25217..26584)
                     /gene="accC"
                     /locus_tag="VQ526_000346"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2554118.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(26596..27018)
                     /gene="fabZ"
                     /locus_tag="VQ526_000347"
     CDS             complement(26596..27018)
                     /gene="fabZ"
                     /locus_tag="VQ526_000347"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2554119.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(27015..27500)
                     /locus_tag="VQ526_000348"
     CDS             complement(27015..27500)
                     /locus_tag="VQ526_000348"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2554120.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(27503..28738)
                     /gene="fabF"
                     /locus_tag="VQ526_000349"
     CDS             complement(27503..28738)
                     /gene="fabF"
                     /locus_tag="VQ526_000349"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2554121.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(28760..29491)
                     /gene="fabG"
                     /locus_tag="VQ526_000350"
     CDS             complement(28760..29491)
                     /gene="fabG"
                     /locus_tag="VQ526_000350"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2554122.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(29525..30445)
                     /gene="fabD"
                     /locus_tag="VQ526_000351"
     CDS             complement(29525..30445)
                     /gene="fabD"
                     /locus_tag="VQ526_000351"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2554123.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(30438..31412)
                     /gene="fabK"
                     /locus_tag="VQ526_000352"
     CDS             complement(30438..31412)
                     /gene="fabK"
                     /locus_tag="VQ526_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2554124.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(31531..31755)
                     /locus_tag="VQ526_000353"
     CDS             complement(31531..31755)
                     /locus_tag="VQ526_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2554125.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(31815..32789)
                     /locus_tag="VQ526_000354"
     CDS             complement(31815..32789)
                     /locus_tag="VQ526_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2554126.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(32789..33223)
                     /locus_tag="VQ526_000355"
     CDS             complement(32789..33223)
                     /locus_tag="VQ526_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2554127.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(33317..34102)
                     /locus_tag="VQ526_000356"
     CDS             complement(33317..34102)
                     /locus_tag="VQ526_000356"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2554128.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            34427..35791
                     /locus_tag="VQ526_000357"
     CDS             34427..35791
                     /locus_tag="VQ526_000357"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2554129.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            35794..36165
                     /locus_tag="VQ526_000358"
     CDS             35794..36165
                     /locus_tag="VQ526_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2554130.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIARDKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            36379..37653
                     /gene="serS"
                     /locus_tag="VQ526_000359"
     CDS             36379..37653
                     /gene="serS"
                     /locus_tag="VQ526_000359"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2554131.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLANYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(37678..38577)
                     /locus_tag="VQ526_000360"
     CDS             complement(37678..38577)
                     /locus_tag="VQ526_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2554132.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPNWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(38687..39235)
                     /locus_tag="VQ526_000361"
     CDS             complement(38687..39235)
                     /locus_tag="VQ526_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2554133.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(39453..40775)
                     /locus_tag="VQ526_000362"
     CDS             complement(39453..40775)
                     /locus_tag="VQ526_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2554134.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPVITALVLSVFVAIAVFGGLKSISKVSTTVVPFMTIIYI
                     LGTLIVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSQVVIAETKKYFDK"
     regulatory      complement(40866..40954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(41118..43454)
                     /locus_tag="VQ526_000363"
     CDS             complement(41118..43454)
                     /locus_tag="VQ526_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2554135.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(43558..44106)
                     /locus_tag="VQ526_000364"
     CDS             complement(43558..44106)
                     /locus_tag="VQ526_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2554136.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPVTTSWLKQIWVTNLIG"
     gene            complement(44103..44405)
                     /gene="zapA"
                     /locus_tag="VQ526_000365"
     CDS             complement(44103..44405)
                     /gene="zapA"
                     /locus_tag="VQ526_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2554137.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            44492..45364
                     /locus_tag="VQ526_000366"
     CDS             44492..45364
                     /locus_tag="VQ526_000366"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2554138.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNSISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKNA"
     gene            45376..45990
                     /gene="lepB"
                     /locus_tag="VQ526_000367"
     CDS             45376..45990
                     /gene="lepB"
                     /locus_tag="VQ526_000367"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2554139.1"
                     /translation="MNLFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            46118..48484
                     /locus_tag="VQ526_000368"
     CDS             46118..48484
                     /locus_tag="VQ526_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2554140.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGFSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGVALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(48532..49815)
                     /locus_tag="VQ526_000369"
     CDS             complement(48532..49815)
                     /locus_tag="VQ526_000369"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2554141.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(49976..50368)
                     /locus_tag="VQ526_000370"
     CDS             complement(49976..50368)
                     /locus_tag="VQ526_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2554142.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNSFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNITEKLDKLVLEND"
BASE COUNT        14810 a        11472 c         9491 g        14621 t
ORIGIN      
        1 ctatcttcca ttatatcata ctttttcagt ctatggtagc tctctttaaa aggctccgat
       61 actgccgcca cctccgcctc cagagaagcc accgccagaa cttccacttc cagaagatac
      121 ggagtaggta cttgctgtat ttgcgacact agcataatgg ctcatttgcg cgcttgaatg
      181 ataaaacata ctgtgccagc cataagctac atagagattg atatctggat tttcaacttg
      241 aatctgatga accttcatca aatgactaac cttgtccgca tagccaaata aggttgcata
      301 gaccaagaga cgattccaaa caacaatact ttccaattca gccttatcca gtcgtgcaat
      361 ctcacgtaac atattttcaa aactggtcca gagatagtag acctcagctc ccgcttcatt
      421 tagaacacca tcacgattat ctagtcgaag cttccaataa tagaaaacag acaaaactaa
      481 ccctagaaaa ccaagtattg gcaaagggag gtaaagatag ccatgaacgt ctaaactgta
      541 caagaacaaa ccaaatccga taaatagggg caagatagtc aagacaccca tacccacttg
      601 caaagccttt tccaaaccag ttaaaggacg atagtaatct gggagccccc agaaggaaac
      661 tcgttttctc actccttctt gcatctggtt caatacctct tcaaaagaag atttgagttg
      721 aagccctctt gcttgaatcc gtttttcatc agaaactttg gctctacgat aaagactatc
      781 agataccttg taatccgcaa acaaattgga aagagtttct tcttttttac ctgaaaaagc
      841 tagatttagg cagtctttct caaagcttga caaaccatct tcttttacta gcctcaaacc
      901 aactgcatct ccttctgaaa tgatagagac attcccacgg tctatcacat ctagcaaggt
      961 agcttgaata agttgatcaa aggtgaattt tccagctccc ttgaccaagg gactcacttc
     1021 ctccaaggag gtcgagtaga ctgcttctga taaaaccata ggctctaatt ccattggggg
     1081 ttcatagaga cgatgatttt tagcatattt gattgaagga gcggtctttc ttctatagat
     1141 aaaatagaaa cagatgctca ataacaagga gatggaaagg atcgaaggga agatccaagt
     1201 aagtagttgt ttactttgat ctttttcttt aacgatcgaa tcctctatct tattaaactc
     1261 ttctaaacga ttccctttca agccctgatc cctagcgcta gcaaaatcag ttcgcggcca
     1321 gtaggcatgc aattcaactc cacgcttagc cggaagattg tctaaacgaa tagtataatc
     1381 aaggttactc ttttcaaccg ttctctctct aaaaagtttc cctgtatgga aaaagagttt
     1441 ctcagccccc ttgtctcccc ttacatgaaa ttcaaacttt tcaatagact ctgaactatc
     1501 tgtcagaggt tgccaattta attcagcgat atcatcataa aggaaaagta aattttttaa
     1561 gttccagacg aggtcaactt caactatgtc gccctcctga cctggattat agactctcac
     1621 agtataacca tccgcttctt ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt
     1681 tttcgcggcc tgaatctttg gatgagggtc aatgtcaaac ccgctaggca tcttaccagc
     1741 acgtccaagt cccacgattt ggcccttaaa gtcctcctca aactggtaaa ctatcttctg
     1801 tctaaactct gccgtattgt ctgcatgaat atacaaatcc ccttgataag agtttatctt
     1861 gaaatcaatg gcaaaaacag agagtggaag aaggcaaaac aagcctaaca ccagtaagaa
     1921 aaaagttttt ttcatcaact aagccccctt tttatctttc ttatcattat agcatttttt
     1981 cgtaagtaaa ggcttactta tgttgaaaaa ttacgcattt ttcgataaaa taggagacag
     2041 ttatttttta atagatcaga ataggagaaa tcatgagaaa aatatactta tctattttca
     2101 caagtctctt gctgatgcta ggacttgtca atgttgctca agccgatgaa tatttacgca
     2161 tcggtatgga agcagcatat gctcccttta actggaccca ggatgatgat agcaacggag
     2221 ctgtcaaaat cgatgggacc aatcagtatg ccaacggata cgatgttcaa atcgccaaga
     2281 aaatcgctaa ggacttaggt aaagaacctt tggttgttaa aaccaagtgg gaaggtctag
     2341 tccctgccct tacttctggt aagattgaca tgattatcgc aggtatgagt ccaactgcag
     2401 aacgcaaaca agaaattgcc ttttcgagca gttactatac tagcgaacca gttttgcttg
     2461 tcaaaaaaga ttctgcctac gcaagtgcta aatctttgga tgactttaac ggtgcaaaaa
     2521 tcacttctca acaaggggtc tacctttata acttgattgc acaaatccca ggtgctaaaa
     2581 aagaaacagc catgggagac ttcgctcaaa tgcgacaagc tcttgaggct ggtgtcattg
     2641 atgcttatgt ttctgaacgt ccagaagcac tgactgctga agctgcgaac tctaagttca
     2701 agatgattca agtagaacct ggtttcaaaa ctggggaaga agatacagct atcgctatcg
     2761 ggcttcgtaa aaatgacaat cgtattagcc aaatcaatgc cagcattgaa accatttcaa
     2821 aagatgacca agttgccttg atggatcgta tgatcaagga acaacctgcc gaagctacaa
     2881 caactgaaga gactagcagt agtttcttta gccaagttgc taaaattctt tctgaaaact
     2941 ggcaacaact cttgcgtggt gctggtatca ctcttttaat ctctatcgtc ggaaccatca
     3001 taggtctcat tattggactt gccattggtg tcttccgtac tgctcctctc tctgaaaaca
     3061 aagtcattta cggcctacaa aaactagtcg gctgggttct caatgtctac attgaaattt
     3121 tccgtggtac gccaatgatt gttcaatcga tggttatcta ctatggaact gcccaagctt
     3181 tcgggatcaa ccttgaccgt acactggctg ctatcttcat cgtttcaatc aataccggtg
     3241 cctacatgac tgaaatcgtc cgtggtggta tcctagcagt tgacaaggga caatttgaag
     3301 ctgcgactgc tcttggtatg acccataacc agaccatgcg taagattgtc ctacctcagg
     3361 tagtccgtaa catcctacct gcaactggta atgaatttgt catcaatatc aaagatacat
     3421 ctgtattgaa cgttatctct gttgtcgaac tttatttctc aggaaatacc gtggcaacac
     3481 aaacctatca atacttccag acatttacaa tcatcgccgt gatttacttt gtcctcacct
     3541 tcaccgtaac acgtatccta cgctttatcg agcgcagaat ggacatggat acctacacta
     3601 caggtgctaa ccaaatgcaa acggaggatt tgaaataatg acacaagcaa tccttgaaat
     3661 taaacacctc aaaaaatcct atggacaaaa cgaagtgcta aaagacattt cactcactgt
     3721 ccacaaggga gaggtcatct ctatcatcgg aagctctgga agcggaaaat cgaccttcct
     3781 acgctccatt aacctacttg aaacaccaac tgatggacaa atcctttatc atggacaaaa
     3841 cgtcctcgaa aaaggctatg acctcacgca ataccgtgaa aagttgggga tggttttcca
     3901 atcctttaac ctctttgaaa atctcaatgt tcttgaaaac acaatcgtcg ctcagacaac
     3961 tgtcctaaaa cgcgaacgca cagaagctga aaagattgcc aaagaaaacc tggaaaaggt
     4021 cggcatggga gaacgctact ggcaagccaa accaaaacaa ctctcaggtg gtcaaaaaca
     4081 acgtgtggcc atcgctcgtg ccctctccat gaatccggac gctattctct ttgatgaacc
     4141 aacatcagct ctcgatccag aaatggttgg agaagtcctc aaaatcatgc aggacctggc
     4201 tcaggaaggc ttgaccatga ttgtcgtaac ccatgaaatg gaatttgccc gtgatgtctc
     4261 tcaccgtgtt atctttatgg ataagggcgt gatcgctgaa gaaggtaaac cagaagacct
     4321 cttcaccaat cctaaagaag accgaacaaa agagttcctt caacgctatc tcaaataaaa
     4381 agaaaaggct gcatcaaccg tgcagtcttt ttgctgtcct caaaatgaaa aggcagattc
     4441 tctatctcta ctacttgaag gtcaagaatc cgccgttatc caaagattaa tcttcaaatt
     4501 tttcataatt tttttcatag aaatcaatta ggcctagggc tgtttcaaat gaaatatttt
     4561 ttacttttgc acgaccctgc gccagagcaa tgatagacat ttcacgcgca ttcgtttctt
     4621 tagagatacg gtagcctgtg attttcttat cacgaaccca gccaacaact gattctactt
     4681 tttcaaagtt tgacttagcc atgtttttct cctattttct tctttataat atttaattgt
     4741 atacgttttt actacttttg tcaataaaaa aaacatatat tcaacgaaaa taattagttc
     4801 agaattttct tacttccttt ttaaatccga taatatatag gttttgctag cctctacacc
     4861 ataaatattt gctaataaaa atactgtatt attaccctct taaggtgcga aattattcaa
     4921 gttcttaaaa aatgcttctc tatttccgta agatagctta tttcttcgat ttaagaactg
     4981 aagatgatag ttctaacctt tagaaatcca agccttcact agctcaaaaa ctcctatctt
     5041 tcttcctcaa taaatcatct gagagatagg tattccttat gtccttaaaa ttcatcactc
     5101 ctttggatat tgaggtgcta gcctagaagc agacacccga ttatctaaac catccttaat
     5161 agagatgaca agacagataa gctcagttaa ttacttatat atctttttaa tatggtatat
     5221 ttgtcaacaa aaagaaatca aatcatacct tatactcaat gaaaatcaaa gagcaaacta
     5281 ggaagctagc cgcaagttgc tcaaaacact gttttgaggt tgcagataga gctaacgtgg
     5341 tttgaagaga ttttcgaaga gtattaaaga cagtgtgact atgcttgtca atagatagga
     5401 aatcaaaatg aaggatggaa atctgagcca aacatcataa gtgctataat gaacagaagt
     5461 aagaattgca cgataagttt tctaaaacca taggaatcgt cccctttctt catcttcatt
     5521 atagcacata tacatcagtt gtacaaataa tatgatattt ttttattagt cctcagacaa
     5581 gcatattata aagcgtttca ttaccattta agttaatata agctggatca aaaccttcca
     5641 ttctacgaat caaacctgca taatcatgct tatctgctaa agcgatccca attaatactg
     5701 ggcctgttcc cttgctagct cgtttgatat actcaaaacg tgtgatatca tcatttggcc
     5761 ccaggatatc atttacaaac tcacgcaaag ctcctggacg ttgtgggaaa ttgaccacaa
     5821 agtaatgttt gataccatca taaatcaagg cacgctcttc catttctggc atacggttga
     5881 tatcattatt tcctccagaa atgatacaac aaatggtttt ccccttaata tattcagcta
     5941 aaacctctaa agaggcgata ctagccgctc cagcaggttc tgcgactatc ccttgcttag
     6001 agtaaaggtc aatcaaggtt tcagaaatca atccctcatc gacacctacc aaagttttaa
     6061 tatgttgacg agttgcttca taggtcaact gacctacctt ttgcacagca atcccatcag
     6121 caaatttatc aatttccttg agtttgactg gacctccagc ctcaaaggca gctttcatgg
     6181 aacgcgctcc attcgcctct actccgataa cctcaatctc tggacttgtt tccttgatat
     6241 aggtagaaac cccggcaatg agaccgccac caccaacagg aaccaagaca gcatcaaaat
     6301 caatcgattc ttttcgagct tcttctaaaa tctcataagc aactgttcct tgacctgctt
     6361 gaacatgagc atcatcaaaa ggatcaataa aggtacgatt ttcagagact gtaaattctt
     6421 gagctgcttt ggctgaggca tcaaaggtat ctccaactag tttaatagtt acaaaatccc
     6481 caccaaaaaa gcgaacctga ccaatctttt gttgtggcgt agtaatgggc ataaagatag
     6541 tagcaggaat tttcatttca ttacaagtat aggctactcc ctgcgcatga tttcccgcag
     6601 aagcgcagac taccccacgt tcacgttctt ccttgctgag ctgggaaatg gcataatagg
     6661 caccacgaat tttaaaggag cgaacacgct gggcattttc ttttttcaaa taaatcttag
     6721 caccatactt ctccgataaa taatgatcgt aatccagtgg agtattcaca accacaccgt
     6781 tcaagacctt gtgagccttg attatatctt ttgaacttaa catataaaac ctctttttct
     6841 ataaaaaaca gtcactaatg actgtttttt agttattcat ctccgaaaac ttcagtaaca
     6901 ctttcatggt atttatgagc catacgaatc gaaaactctt taggaagttc ataacgattt
     6961 gtggaaatcc aatcatataa ccattctacc aaactttctt cactatcatt acgatggaag
     7021 ataaaatagt ttaaaatttc tttatattct acatcattca aatccaacgt gtctacaaat
     7081 tgactacgag gaacaaaatt agaatctaat tttcttagtt gatctgtcaa ttctcccaat
     7141 tgtaaatcca actgaaaaaa tagtttttgc agttgcctat cagtttcatc acatcctgtc
     7201 ttgatagaaa acaaattggt aatattcata tgatttcaac ccctaaataa gtacatctaa
     7261 tatcccacga atagctgaag ctaaagtttt cgcagtccag tcatttaaac ccgattcttt
     7321 caatccagat gcaataattt gctctatttg atataaatcg ccatctatcg tctcatttat
     7381 tctgcgaaga ggaccttcaa tataggataa agcttctccg aatctaacag ctgatttagg
     7441 tcctaatgat ttaccaacta tatttgttaa tgttttttat ttctcaaaat ccatttcgct
     7501 atataacgag cagatttagt cgcatcagct ctctcaaacg gagaatgaat ttcaagacct
     7561 tcttctctag ctatttgtgc caatttgaag aattgttcaa aggtcatatg caacctacca
     7621 tcttcaaaat tagcatcatc ataaatgtta attacagcat tttcatcata cgaccaatct
     7681 gctttatttt ggtctgctag cactattacc gacggtgtaa ttacattata caactagaac
     7741 caataataac gatgcaattt ttctcataat ctttttcata aaatcatctc cagcgctata
     7801 tatttctaat tagttataga ttttgaatgc atcatcgtcg tttttaccaa cgaatggcat
     7861 tgctttacgc aattctgcac caactttttc aatttcaagg ttagctgctt gttcacggta
     7921 agcagtcaat tttggacgtc cagctttata gtcatttaca aagtcatttg caaacttacc
     7981 attttggatg tctgccaaga cagccttcat attttcttta acttgttcag tgattacacg
     8041 tggacctgat acatagtcac cgtattcagc agtgtttgaa atagactgac gcattttctt
     8101 gaatccacct tcgtagatca agtcaacgat caatttcatt tcgtgaagaa cttcaaagta
     8161 agccaattct ggagcgtaac ctgcttctgt caagacttcg aaacctgctt cgataagggc
     8221 agtcaaacca ccacaaagta cagcttgttc accaaacaaa tcttcttcag tttcttcttt
     8281 gtaagttgtt tcaagaagac ctacacgagc cgctccaaca cctttacacc agtccatagc
     8341 aatgttttta gcatttcctg ttgcatcttg gtatactgca taaagagctg gaacaccaaa
     8401 tccttcttcg taagtacgac gtaccaagtg tcctggtcct ttaggagcac acatgaagac
     8461 atctacatcc gcaggaactt tgataaattc aaagtggatg ttgaaaccat gggcaaatcc
     8521 aactgcgttt ccagcttcca agtttggagc gatttctgct tcgtacaatt cttgttgaat
     8581 ttcgtctggc gccaagatca tgataacatc agccaactta gtagcttctg ctactgtgta
     8641 agtatcaaat ccatcttctt ttgctttatc aaaagattta cctggacgta caccgataat
     8701 aacgtcacga ccggaatcac gcaagttttg agcatgcgca tgcccttgtg aaccataacc
     8761 gataacggcg atttttttac cgtcaagtgc tgctacttta acatcttttt catattccat
     8821 ttgaactgtc atagcttttc tctcttttct attatttatt gccttttagg ctggtttaat
     8881 aaatttaagt tggattttta atcgcgggta aatccagttg cacccgttcg agcaatattg
     8941 cgaataccgt atgggcgaat gactcgcaat agggcttcgc tcttttctgc atttcccgtc
     9001 atctgaatgg taatcgagct tggcgctacg tctaccactg ttgcacggaa aggttgaata
     9061 atcgctaaaa tctcagctct cttctcagct ggcgctgaca tcttaaccaa aatcacctcg
     9121 cgctccaaat gaggcttgtc tgtaatatct cgaatgcgaa tcacatcaat ctgacgattg
     9181 agctgtttga tgatttgctc cacttcatca tgagaagcaa catcaataat aatagtgata
     9241 cgcgatacat tcggatcttc tgttgctcca acagagatgc tttcaatatt aacctgacga
     9301 cgagatagga cacctgtaaa gcgattgagt actcctgaac gattttgtag ttttgctgtt
     9361 aacattctac gcatggaact tcacccccaa catctcatga ttactcttac cagccggtac
     9421 cattggtaac acctgttcct tacgagaaat atctacctca attagcatag gaacatcctc
     9481 agtgatgact tcaaggtctt gagccaaggt ctcaggattg tcaaacttat agtttttaat
     9541 accataagcc tgcgccatca attggaaatc aggaagggta tcaaagaccg actctgatgt
     9601 tctgccttca tagaaggatt cctgccactg gcgaaccatt ccaagtgaat gattgttcag
     9661 cataaccacc ttgattggca ccttgtaaat attcaaaata gccaactcct ggttggtcat
     9721 ttggaaacca ccatccccaa caaacaagac tacttcctta tctgggttag caattttagc
     9781 accgattgct gctggaattc caaagcccat tgttcccaaa ccacctgaag tcactaactg
     9841 acgctcattt tggtagggat aatactgagc tgtccacatt tggtgttgac caacgtctgt
     9901 taccacaatg gcatctccat tcgtcaattc accaattcgt tcaataactg cttgcggttg
     9961 aaccacacgc tctttcttgt cataagaacg aacacgattc ttgtctttag tgactttctc
    10021 aatccacttt tcagtattgt tgtgaactgt tggttctgct agcaacattt gcaaggcctt
    10081 cttagcatct ccaactacag gaatgtctgc actgataatc ttgccaatct cagctgggtc
    10141 aatatcaatg tgggcaacct tagcattctt agcgaaagtc ttaggattcc ccgtcaaacg
    10201 gtcatcgaaa cgagaaccaa tactaatcat aaagtccgct tccgtcatgg caatatttgc
    10261 tgcgaatgac ccgtgcatgc ctcccattcc aagaaagagt gggtgactcg ttgcaatcgt
    10321 tccttgtccc aaaagactgg ttaccactgg aatttgatag cgttctgcaa attcatttag
    10381 ttccgtagca gcctcagcat aactaattcc accaccagct aacaagactg gctttttagc
    10441 cttggacaat tgcttcaaga ttttcttgat ttgcatatca ttcggctcaa gagtcggctg
    10501 ataacttggt aaattcactt ctggtgaata aatgaagtct gtttctaaag cagatatgtc
    10561 ttttggtagg tcaattacaa ctggccctgg acggcctgta gttgcgatat ggacagcttc
    10621 cgtaatgata cgcggaatat cagctgtctc acgaacttgg taattgtact tagtgattgg
    10681 catggtaatt cccacgatgt ctgcctcctg aaaggcatcc ttcccaatcc ctgctcgcgc
    10741 cacctgacct gtaaagacca aaaggggaac gctatcgctc atggcatccg caatccctgt
    10801 aatggcattt gttgctcctg gtccactagt gacgacggca acacccaact ttccagttga
    10861 tttggcataa ccttcagctt catgcaaaca accttgctca tggcgcccta gaatgtggcg
    10921 aatgccttta aaattatata tcgcatcata aaaaggcaaa accgcaccac caggataacc
    10981 aaagatggta tcaactccta aatcacgaag tgtttccaaa actaggtccg accccgtctt
    11041 aggagattct aaactgattt tctccattat tcccctttct ttcctcttaa aaataacttg
    11101 ttactatcat accatttttt caaaattttt caagataaaa gaagaaattt tctgaatttt
    11161 ctattttaac gtttatttat gaatgttatt tttgttttta ttaaaaagat accgatttca
    11221 tttactaaaa aagaaaaaag aactgatttc tcagtccttc attaatctta ttccacacta
    11281 aataggtatg ggtaaacagg ttgttgacct tggtgaatct cgacttcaac gtcttcgaat
    11341 tcttctacga tttcttgagc gatttcattg gcaagttctt cgcttccgtc ttcaccgaca
    11401 tagaaggtta cgatttcact gtcttcatcc aacatatgtt tcaaggtttc agtcaatgtt
    11461 tggtgcatat cagggtttga cacaagaatt tttccatcca ccatacctag attatcgttt
    11521 tcatggattt ctaagccatc gatcgttgta tcacgcacgg ctgttgtgac gcttccgcta
    11581 acaacatcgc taagagcagc tgtcatacgc tcttggtttt cttcaatgga cttgcttgga
    11641 tcaaaggcaa gaagacttgt cataccttga ggaagagtgc gagcctctac cactactgct
    11701 ggttgctcca aaacttctgc cgcagattga gctgccatga agatgttctt gttgtttggc
    11761 aagaagatga tgttacgggc attgacctgt tcaacagcct tgataaagtc ttctgttgaa
    11821 gggttcatgg tttgaccgcc ttcgataaca taatccacgc cttgagaaca gaagatatct
    11881 gctagacctt taccagccac cacagcaatc aaagcatact ctttttcttc agccgacttg
    11941 ataacttgag tagcttcttt ctcaacctgt gcttcgtgtt ggttacgcat attgtcaact
    12001 tttaccttga ccaagctacc atatttgaga ccttcttgca taacaagtcc tggatcttct
    12061 gtatgaacat ggactttgac aatttcatca tcgttaacaa cgaggagaga atccccaagc
    12121 tcatccaagt agttacggaa ttcatcgtag tcaaaatctt tagcataggt tggaccttgc
    12181 ttaagagcta ccatgatttc agtacagtaa ccaaacgtga tgtcctcagt cgctacgtga
    12241 ccagctacag acttatgatg ctctacattg atcatctcac tcatgttggc aggagtcgct
    12301 acaaagtcct cagatgcaat atattcgcca gtaagggctg aaaggaaacc ttcgtagatg
    12361 aagaccaatc cttgaccacc tgagtccaca acgccaactt ctttcaatac tggaagcatg
    12421 tctggtgttt tagctagagc tgttttagca ccttccaagg ctgcgcgcat gacttcaaca
    12481 gcgtcatctg tttgctcagc ttttttctta gcaccgatag cagctccacg agaaactgtt
    12541 aaaatcgttc cttcaacagg tttcatcact gccttatagg caacttccac acctgattgg
    12601 aaggccagag ccaagtcttg acctgttaac tcgtctttat ccttgatagc ttgggaaaat
    12661 ccacggaaaa gctgagacgt aatcactcct gagttcccac gcgcacccat caaaagccct
    12721 ttggcaagaa tgctcgctac ttctccaact gtagaagctg gcttgtctgc aacttcttta
    12781 gcaccatttt caatggtcat tcccatattt gtcccagtat ctccatctgg gactggaaag
    12841 acgtttaatg aattgacata ttcagcttgc ttattcaagc gagttgatgc agcctgcacc
    12901 atttcttgaa ataagctagt agtaattttt gacacggtta ttctcctaca actttgatat
    12961 tttgaatgta gacatttaca gtctgagcag taattccaag ctggttttcc aagctaaagg
    13021 caacacgctc ttgaatgttt tttgacactt cactaatctt tgttccgtag cttaacacgg
    13081 tatatacatc aactgcaata ctgccatctt cggctgcctt tacgacgaca cctttagaat
    13141 aattttcctt acctagcagg gcttggaaat tatctttgag ggcattttta ctagccatac
    13201 cgaccacacc aaaaatctca gttgctgctc cacctacgac ggttgcaatc acttcatctg
    13261 ttagttcgat ttgaccatct tttgtattaa tttttacagt catccttttt acctcaacta
    13321 gttgatactc tattttatca tatttcagcc caagtgtaaa agcagaatga tgtatcagcg
    13381 gatatttact ctatttttca aacgatgtta tacccacaat aaaagaaaaa agaccctaag
    13441 gtctcctttg cttttattat taaacgcgtt caactttacc tgatttcaaa gcacgagctg
    13501 aagcccaaac ttttttaggt ttaccatcga taagaacagt aactttttga aggtttggtt
    13561 ttacggcacg ttttgtttgg ttcatcgcgt gtgaacggtt gtttcctgat acagtcttac
    13621 gacctgtaaa gtaacatact ttagccattg tgttttcctc ctattagatc taatatagcg
    13681 gatgtgctag caccacatac cgtactatgt tatcacactt tcttgttttt tgcaagggaa
    13741 ttggaagatt ttttatttgt gtcttaaatc aggtcttgcg tgacattttc tgctctccac
    13801 atgccattgt tgattaacag aacaccagaa ttaaaattat gtgtataaaa atcatctcta
    13861 actgcagcta agggtatagc cgtcaagtcc aaatcccaca gctcatctat cgattttctt
    13921 acaacaatat ctgaatccaa atacagtaca cgagactcgc ttacatactt tggaataaaa
    13981 tacctaaaaa aagccgcata tgaaagtccc tcaaagggga gacgataacc tttcagaata
    14041 ctactgtcaa tctgaacatt cacaatctca ctattcaaag tctctagtct tttttccatc
    14101 aattggaacc attctcgcgg aaggtcatca ttaaaaacat aaaacttaag attataatga
    14161 tgaacacaaa gagattttat tgttgtttca actttatcca tataagcatt atctgcacct
    14221 aagacaatcg cttttttctc ttctttcact ctttatctta tttcttttta ttcccatcat
    14281 attattccca tcatatgttt ctacgtaacc attattttcg cctattcgtt cgtaaaacca
    14341 taccagtgga gattttagat gaagtcccat tacggtttac attttttaca ttacgacacg
    14401 gagttttaca aatcgatttc atttgccaaa cgtagttagt gaggcagtta gctagttcgc
    14461 caaatagcga ctagcgtcca acaatttgga actttagttc caattgttgg tactgagtca
    14521 catcttctcc tctaactcta cgtctggata cttgtccgca aaccagcgga gggcaaagtc
    14581 attttcaaag agaaagactg gttggtcaaa acggtctttg gctaagatat tgcgacttga
    14641 cgacatccgt tcatccaagt cctcaggctt gatccaacga acggtctttt tacccattgg
    14701 gttcataact acttccgcat tgtactcgct ttccatgcgg tgtttaaaga cttcaaactg
    14761 gagttgacct acagcgccta gcatgtactc acctgtttgg taattcttat aaagctgaac
    14821 ggctccttct tgcaccaatt gctcaatccc cttgtggaag gatttttgct tcataacatt
    14881 cttagcagaa actttcatga aaatttcagg agtaaaggtt ggcagtggtt caaattcaaa
    14941 cttgtttttt ccaaccgtca aggtatcccc aacctgataa gtaccggtat cgtaaacccc
    15001 gataatatca cctgccacgg cattggtcac attctcacga ctctccgcca taaactgggt
    15061 aacattagat agtttggcac ccttaccagt acgagggaga ttgacactca tgccacgctc
    15121 aaattcgcct gatacgatac ggacaaaggc aatacggtca cggtgacgag gatccatgtt
    15181 ggcttggatt ttaaagacaa agcctgagaa atccttgtca taaggatcca caatttcacc
    15241 gtctgttttc ttgtgaccat gtggttctgg agcaaacttg aggaagattt caaggaaggt
    15301 ctgcacacca aagtttgtca gggctgaacc gaaaaagaca ggcgtcaatt ctccagccag
    15361 aatagcttcc tctgaaaact cattcccagc ttcatttaaa agctcaatgt catccttgac
    15421 ttgctcgtag aaaggattgc taccaaaaag tttgtctcca tcttctaggc tagcaaaacg
    15481 ctcatcccct ttgtaaagct ctaaacgttg gttatagagg tcatacaagc cctcaaaggc
    15541 tttccccatc ccgataggcc agttcatagg gtagctagca atgcccaaga tttcttccaa
    15601 ttcttgcaag agatccaaag gctcacgacc gtcacgatcc aacttgttca taaaggtaaa
    15661 gactggaatg ccacgatgtt tcacaacctc aaacaatttc tttgtttgag cctcgatccc
    15721 cttggcagag tccacgacca tgaccgcagc atccaccgcc atcaaggtac gataggtatc
    15781 ttctgagaag tcctcgtgcc ctggcgtgtc taagatattc acgcgcttgc cgtcgtagtc
    15841 aaattgcata acagatgaag taacagaaat cccacgttgc ttctcgatat ccatccagtc
    15901 agatttagca aaagtccctg ttttcttccc ttttaccgta ccagcatcac gaatctcacc
    15961 cccaaagtag agtaactgct cagtgatggt tgttttcccc gcgtccgggt gggagataat
    16021 ggcaaaggta cgacgtttct taatttcttc ttgaatattc ataagttctc tttctttgat
    16081 tctctatttt tcttgtttca atagctgaga atgattttta cattggattt taccattcct
    16141 ttcgacactc cattatatcg gattttagca tttttttcaa tttctatttc ttttcacttc
    16201 cccctccctt atttatagga aaatatggta aaatagaaca gactaaaaat catcatttca
    16261 cgaaaggatg caagatgaaa attacgcaag aagaggtaac acacgttgcc aatctttcaa
    16321 aattaagatt ctctgaaaaa gaaactgctg cctttgcgac caccttgtct aagattgttg
    16381 acatggttga attgctgggc gaagttgaca caactggtgt cgcacctact acgactatgg
    16441 ctgaccgcaa gactgtactc cgccctgatg tggccgaaga aggaacagac cgtgatcgct
    16501 tgtttaaaaa cgtacctgaa caagacaact actatatcaa ggtgccagct atcctagacg
    16561 atggaggaga tgcctaatga cttttaacaa taaaactatt gaagagttgc acaatctcct
    16621 tgtctctaag gaaatttctg caacagaatt gacccaagca acacttgaaa atatcaagtc
    16681 tcgtgaggaa gccctcaatt catttgtcac catcgctgag gagcaagctc ttgttcaagc
    16741 taaagccatt gatgaagctg gaattgatgc tgacaatgtc ctttcaggaa ttccacttgc
    16801 tgttaaggat aacatctcta cagacggtat tctcacaact gctgcctcaa aaatgctcta
    16861 caactatgag ccaatctttg atgcgacagc tgttgccaat gcaaaaacca aaggcatgat
    16921 tgtcgttgga aagaccaaca tggacgaatt tgctatgggt ggttcaggtg aaacttcaca
    16981 ctacggagca actaaaaacg cttgggacca cagcaaggtt cctggtgggt catcaagtgg
    17041 ttctgccgca gctgtagcct caggacaagt tcgcttgtca cttggttctg atactggtgg
    17101 ttccatccgc caacctgctg ccttcaacgg aatcgttggt ctcaaaccaa cctacggaac
    17161 agtttcacgt ttcggtctca ttgcctttgg tagctcatta gaccagattg gaccttttgc
    17221 tcctactgtt aaggaaaatg ccctcttgct caacgctatt gccagcgaag atgctaaaga
    17281 ctctacttct gctcctgtcc gcatcgccga ctttacttca aaaatcggcc aagacatcaa
    17341 gggtatgaaa atcgctttgc ctaaggaata cctcggtgaa ggaattaacc cagaggttaa
    17401 ggaaaccatt ctaaatgccg ctaaacactt tgaaaaattg ggtgctattg tcgaagaagt
    17461 cagccttcct cactctaaat acggagttgc cgtatactac atcatcgctt catcagaagc
    17521 ttcatcaaac ttgcaacgct tcgacggtat ccgttacggc tatcgcgcag aagatgcaac
    17581 caaccttgat gaaatctatg taaacagccg aagccaaggt tttggtgaag aagtgaagcg
    17641 ccgtatcatg ctgggtactt tcagtctttc atcaggttac tacgatgcct actataagaa
    17701 ggctggacag gtccgtacac ttatcattca agatttcgaa aaagtcttcg cggattacga
    17761 tttgattttg ggtccaactg ctccaagtgt tgcctatgac ttggattctc tcaaccatga
    17821 cccagttgcc atgtacttag ccgacctatt gaccatacct gtaaacttgg caggactgcc
    17881 tggaatttcg attcctgctg gattctctca aggtctacct gtcggactcc aattgattgg
    17941 tcccaagtac tctgaggaaa ccatttacca agctgctgct gcttttgaag caacaacaga
    18001 ctaccacaaa caacaacccg tgatttttgg aggtgacaac taatgaactt tgaaacagtc
    18061 atcggacttg aagtccacgt agagctcaac accaattcaa aaatcttctc acctacttct
    18121 gcccactttg gaaatgacca aaatgccaac actaacgtga ttgactggtc tttcccagga
    18181 gttctaccag ttctcaataa aggggttgtt gatgccggta tcaaggctgc tcttgccctc
    18241 aacatggaca tccacaaaaa gatgcacttt gaccgcaaga actacttcta tcctgataac
    18301 cccaaagcct accaaatttc tcaatttgat gaaccaatcg gatataatgg ctggattgaa
    18361 gtcaaactag aagacggtac gaccaagaaa atcggtatcg aacgtgccca cctagaggaa
    18421 gacgctggta aaaacaccca tggtacagat ggctactctt atgttgacct caaccgccaa
    18481 ggggttccct tgattgagat tgtatctgag gcagatatgc gttctcctga agaagcctat
    18541 gcttatctga cagccctcaa ggaagttatc cagtacgctg gcatttctga cgttaagatg
    18601 gaggaaggtt cgatgcgtgt ggatgccaac atctcccttc gtccttatgg tcaagagaaa
    18661 ttcggtacca agactgaatt gaagaacctc aactccttct caaacgttcg caaggggctt
    18721 gaatacgaaa tccaacgtca agctgaaata cttcgctcag gtggtcaaat tcgccaagaa
    18781 acacgccgtt acgatgaagc taacaaaaca accatcctca tgcgtgtcaa ggaaggggct
    18841 gcagactacc gctacttccc agaaccagac ctacccctct ttgaaatttc tgacgagtgg
    18901 attgaggaaa tgcggactga gttgccagag tttccaaaag aacgtcgtgc gcgttatgta
    18961 tctgaccttg gtttatcaga ctacgatgct agtcagttga ctgctaataa agtcacttct
    19021 gacttctttg aaaaagctgt tgccctaggt ggtgatgcca aacaagtctc taactggctc
    19081 caaggggaag tcgctcagtt cttgaatgct gaaggtaaaa cactggaaca aatcgaattg
    19141 acaccagaaa acttggttga aatgattacc atcatcgaag acggtactat ttcatctaag
    19201 attgccaaga aagtctttgt ccatctagct aaaaatggcg gtggcgcgcg tgaatacgtg
    19261 gaaaaagcag gtatggttca aatttcagat ccagctatct tgatcccaat catccaccaa
    19321 gtctttgccg ataacgaagc tgctgttgcc gacttcaagt caggcaaacg taacgccgac
    19381 aaggccttta caggattcct tatgaaggca accaaaggcc aagccaaccc acaagttgcc
    19441 cttaaactac ttgcacagga attggcgaag ttgaaagaaa actagacaga acaaaaccag
    19501 ccctaaggtt ggttttttct tgacaaatag aaagaaaacg tttacaatat tgcgtgtaaa
    19561 gtttccaaac aagaaaacta aaaatcgcaa caataaggag ttatttatat ggctacaatg
    19621 aaggcagctc gctggcatgc agcgaaagat gttcgtattc aagaaataga agttcctgaa
    19681 gtacttccgc accaagtaaa agttgcagtt aagttcacag gaatttgtgg tactgacctc
    19741 catgaatttt tagatggacc tatctttatc ccaacagatg agcacgtcta ttctggtcaa
    19801 aaagcaccgg taacactggg acatgaattt tctggtgaaa tcatagaagt cggaagcgat
    19861 gttacccgtg ttaaagttgg tgatcgtgtt gctgtggaac caattctagc taaaaataac
    19921 ttagttggtg attataattt ggatccaaac cttaattttg tcggtttggc tgcagacggt
    19981 ggatttgcca aatattgtgt tttagacggt gacttagtac atgtcattcc agatagcttg
    20041 agctatgagc aagctgctct tactgaaccc gctgctgttg cagtctatgc tgttcgtcaa
    20101 tcttctttga aggctggcga tacagctgtt gtctttggtt taggcccgat tggtctatta
    20161 attgtagaag ctcttcgtgc agctggtgct tctaagattt atgctgttga actatcacct
    20221 gaacgtcaag caaaagctga agagttagga gctatcgttg tccgtccgga agaaggtgaa
    20281 acagctgttg aagctattca tcgtttaaca aacggtggtg tagatgtttc ttatgaagta
    20341 acgggtgtgc cagtagtttt aggacaagcc ttagctgccg tacataaagc aggtgagtgc
    20401 atggtggtct ctatctggga acgagaagca aatattaatc cgaatgaatt tgcaatccaa
    20461 gaaaagacac taaaaggaat catcgcttat cgtcatatct tccctaaagt attagaattg
    20521 atggaacaag gctacttctc tgctgaaaaa ttagttacta agaaaattaa attggaaaat
    20581 atcgtcgaag aaggctttat cgaattaact caagataagt cacaaattaa aattttggta
    20641 gaaccagaat aaaaaatcaa tctttttaag acaatactaa caggtcggag taaacttact
    20701 tcggcctttt gaaatataat actcttcgaa aatctcttca aaccacgtca gcgtcacatt
    20761 accgtatata tggttactga cttcgtcagt tctatccaca acctcaaaac agtgttttga
    20821 gcagcctgcg gctagcttcc tagtttgctc tttgatttcc attgagtatg agaagaaata
    20881 tagtggatac tctttaacat taaccactcc acctgactag ccaatattct ttttcgctcc
    20941 taccaactcc caataactat tttggcttta tttccagagt attttatggt aaaatgaaga
    21001 gtaataatat ttgttaaaga ggtaaaaaca tgattgaagc aagtaaatta aaagctggta
    21061 tgacctttga aacagctgac ggcaaattga ttcgcgtttt ggaagctagt caccacaaac
    21121 caggtaaagg aaacacgatc atgcgtatga aattgcgtga tgtccgtact ggttctacat
    21181 ttgacacaag ctaccgtcca gaggaaaaat ttgaacaagc tattatcgag actgtcccag
    21241 ctcaatactt gtacaaaatg gatgacacag catacttcat gaatacagaa acttacgacc
    21301 agtacgaaat ccctgtagtc aatgttgaaa acgaattgct ttacatcctt gaaaactctg
    21361 atgtgaaaat ccaattctac ggaactgaag tgatcggtgt caccgttcct actactgttg
    21421 agttgacagt tgctgaaact caaccatcta tcaaaggtgc tactgttaca ggttctggta
    21481 aaccagcaac gatggaaact ggacttgtcg taaacgttcc agacttcatc gaagcaggac
    21541 aaaaactcgt tatcaacact gcagaaggaa cttacgtttc tcgtgcctaa tctctagaaa
    21601 gaggtcattc tatgggaatt gaagaacaac ttggcgaaat cgttatcgcc ccacgtgtac
    21661 ttgaaaaaat cattgctatc gctactgcaa aggtagaggg tgttcactct ttttcaaaca
    21721 gatcagtgtc tgataccctt tcaaaacttt cactcggccg tggcatttat cttaaaaacg
    21781 tggacgaaga actcacagca gatatctatc tctaccttga gtacggagta aaagttccta
    21841 aggtagcggt tgctatccag aaagctgtca aagatgccgt ccgtaatatg gctgatgtag
    21901 aactcgctgc tatcaatatt cacgttgcag gtatcgtccc agataaaaca ccaaaaccag
    21961 aattgaaaga tctatttgac gaggacttcc tcaatgacta gtccactatt agaatctaga
    22021 cgccaactcc gtaaatgcgc ttttcaagct ctcatgagcc ttgagttcgg tacggatgtc
    22081 gaaactgctt gtcgtttcgc ctatactcat gatcgtgaag atacggatgt acaacttcca
    22141 gcctttttga tagacctcgt ttctggtgtt caagctaaaa aggaagaact agataagcaa
    22201 atcactcagc atttaaaagc aggttggacc attgaacgct taacgctcgt ggagagaaac
    22261 ctccttcgct tgggagtctt tgaaatcact tcatttgaca ctcctcagct ggttgctgtt
    22321 aatgaagcta tcgagcttgc aaaggacttc tccgatcaaa aatctgcccg ttttatcaat
    22381 ggactgctca gccagtttgt aacagaagaa caataaaaag aaaaagtgtt tgatagatat
    22441 caaacacttt tttctttgcc tcccactatc taaaaaaatg ataatagata taattgtaaa
    22501 caaaaatcca gataggtttt gcatgattga gaaagttaaa aaaactatgg cagagaatcg
    22561 ttaatctcag attgtcggta gaacgataaa caagggcaaa aaagaaacca atcagactat
    22621 aatataataa actaattgga tctctgtgag atagtatcaa atggctaatc ccaaagatga
    22681 tagcagatag gataacatcc aaatagtact tggactaggg aaagaaggta ttcataaaat
    22741 accctctatc aagagtctcc tcaaaaacag gaccgatgat tacaggcagg acaaaagata
    22801 agatagtcga taaaaaggtt ggttgtccat ttgaaaaaag cacggtaaaa tactcatcat
    22861 gaatattcct atgattaatc aaatgagcat agcgtgccca aaaattaccg agaatctgat
    22921 aaaccacata agttgcaaat aagtagaaga caaatgacca gttccagctc tttttctcaa
    22981 agataaagag catctttttc ttttttaacc tccaaattaa tagaaggaaa cttcccacta
    23041 atcccattgt taaaataaga gaatagacat cagctcctaa ccctaaaatg atcgtcacat
    23101 acaatccaat tgtttgtggt aaataggtag atagtaaaat aataagcaaa aatattccaa
    23161 attgtcttag tttttttgtg tttctcatcg tacttttttg aaagattacc ctgctcggaa
    23221 gccgtacttc caagcatcta tataagaatt aagtgcccct tgcctcatat agggagcaaa
    23281 ttctctataa tataaccatc tactatatcc atcttcccaa acagcaagac cacctgaagt
    23341 ttgctccaag tcctcagttg aaagaactgt aaatgtattt gtacctgtca ttgcaagtac
    23401 cttcttaaaa tagattgttg taggctcaca tttatagtat atttcttttt ttgtctattt
    23461 tatagcccat ctcctcaact ggcaattttt cgacctgaat tacatttttc cataaaaaat
    23521 gagacctttc tagtctcatt tagtcattct tagtattttc taaatcgttg atagcgttct
    23581 tccagcaact cttctagcgg tttttgtgaa agtctagcca gctctgtttg gagttctttt
    23641 ttgacactct taatcagttc tttactagaa agtcctattt cagaaatcac cttatctacc
    23701 acgtccattt ctaacagttc atgcgaagtg attttcatca gttctgctgc ttccatagcg
    23761 cgagtaccgt ccttccataa aatggaagca aagccttctg gactgagaat ggcatagata
    23821 gaattttcca gcatccagac acggtccgcg acagctagag ccagagcccc gcctgaacca
    23881 ccttcaccga taataatggc gataatagga actttcaggt cactcatttc catgagattg
    23941 cgagcgatag cttccccttg accacgttct tccgctccga caccaggata agcacctgct
    24001 gtattgataa aggtcacaac tggacggcca aatttctcag cctgtttcat caaccgcagt
    24061 gcctttcggt agccttctgg atgtggttgg ccaaaattcc gtttgaggtt gtcttgcaaa
    24121 ctcttgcctt tttggatacc aaccactgtt acagcttgat ctccaagcca accaatacca
    24181 ccaacaactg caccatcatc acgaaaagaa cggtcaccat gtaattggat aaattcatca
    24241 aaaatgcctg tcgcaaagtc caaggttgtc aagcgactct gctcacgcgc ttctctgact
    24301 atttttgcaa tattcatcta ggactccctc catgcaatct gactaggcta gcaatcgtat
    24361 ctggtaagtc tcttcttttg acaatagcat ccacaaagcc atgttctaat aggaattctg
    24421 ccttttggaa atcctcaggc aagctttcac gaaccgtatt ttcaatcaca cgacgcccag
    24481 caaaaccaac caagctctgt ggttcagcca gaatgatatc gccttccata gcgaaagaag
    24541 ctgtcacacc accagtcgtt ggatctgtca aaatggtcag gtaaaagaga ccaacatttg
    24601 aatggcgttt aaccgccgca gagatcttag ccatctgcat gagactcatg attccttcct
    24661 gcatacgggc tccaccagag gctgtgaata ggacaactgg caatttttcg acagtcgcat
    24721 actcaaacaa acgagtgatt ttttcaccta caaccgtacc catagaagcc atgataaagt
    24781 tagaatccat aatcccaaga gccacagtct gacctttaat aagagcagtt cctgtcacaa
    24841 cggcttcatg cagacctgtt ttttcacgca tagatgccag tttcttttgg taaccaggga
    24901 aatgcaaggg atccttgctt tcaatccctg taaacaattc tttgaaggtt cccatatcaa
    24961 tcgtcaaagc caagcgttct tgggcagaaa tacgaaaggt atagctacag tgcggacaga
    25021 tacgttcact tcccagatcc ttctgataga tggtatgctt acagcctgga cactgggaaa
    25081 ataattcatc tggaacctct ggcttagctt gaggtttttc cctaaccgaa cgattgggat
    25141 tgattcgaat atacttatct tttttactaa atagagccat tgattcccct tttcggttta
    25201 aactcttaaa gtcattttat tctttttctt gatatttagg taagaaggtt tccatcaaga
    25261 aggaagtatc ataatcccca gcaatgacat tgcgatctga aatgaggtca agctggaaat
    25321 ctgcattggt ctgcactcct tcaatctcta attcatagag ggcacgttgc attttcatca
    25381 aggcgtcaaa acgattttcg ccgtgtacta tgattttggc aatcatacta tcataataag
    25441 gcggaatggt ataacctgga taaactgctg aatccacgcg caaaccaact ccaccacttg
    25501 gcagatagag attagtaatc ttacctggac ttggagcaaa gttaaaggct gggttttctg
    25561 cattgatacg acactcgatg gcatgaccac gtaggacaat atcttcttgc ttaacagaca
    25621 aaggctgacc tgccgcaatg caaatctgtt ccttaacgat atcaacacct gaaacaaact
    25681 ctgttactgg atgttctacc tgaacacgag tattcatctc catgaaatag aaattgctac
    25741 ttgcttcatc aagaagaaat tcaatggttc ctgcattctc atagccaaca aactctgccg
    25801 ctcgaacagc agcagcacct atttcatgac gcagcgtttt tccgattgca atcgagggac
    25861 tttcttccaa aaccttttgg ttattccttt gaagagaaca atcccgttca cccaagtgaa
    25921 tcacatgtcc atgctcatca cctaggattt gaacctcaat gtgccgagct ggatagataa
    25981 cccgttctat gtacatggca ccattgccat aattggcctt ggcctcacta gaggcagttt
    26041 caaaggcaga aacgaggtca tctggttttt caaccttacg aatcccttta ccacctccac
    26101 ctgctgaagc cttgagcata acaggatagc caattttttc agcaacaatc aaagcttctt
    26161 cagagttatg cacttctcca tctgaacctg gtataacagg cacacctgct ttaatcatct
    26221 gagcacgcgc attgatctta tcccccatca tatccataac atgaccagat ggaccgataa
    26281 acttgatacc tacttcttca cacatggtcg caaatttgga attttcactg agaaatccaa
    26341 aaccagggtg aatagcttct gcctcagtca agactgcagc tgatagaact gcattaatat
    26401 tgagataaga ctctgttgcc ttgccaggac caatacaaac tgcttcatct gccaaaagcg
    26461 tatgaagagc ttccttatca gcagttgaat aaaccgctac cgtcgcaatc cccaattcac
    26521 gtgccgcacg gataatacga accgcaattt caccacgatt ggcaattaaa atttttcgaa
    26581 acatggagaa cctccttagt tcccaattgc aaaagtaagg ataccactgg ctgcaagctt
    26641 gccatccact tcagcctttg cttcaaccac agctatggtg ccacgacgtt ttacaaaagt
    26701 cgctgtcata accaattggt cgcctggtac aacttgcttc ttgaacttaa ccttgtccat
    26761 accagcgtaa aagaccagtt ttcctttatt ttcaggtttt gataactcca acacaccagc
    26821 agtttgcgcc aaggcttcca taatcagaac acctggcata actgggtatt gaggaaagtg
    26881 gccgttaaag aaaggttcgt taatggtcac atttttgata gcaacaatgg tatcctcgct
    26941 cacttccaag acacggtcca ctagaagcat aggataacgg tggggaagag cttctttgat
    27001 tccttgaata tcgatcattt gatacgtacc aatcctttac caaactcaac catttcttcg
    27061 ttagagacga gaatttccgt taccacacca tccttaggag ctgggatttc attcatgact
    27121 ttcatggctt cgataattac caatgtttga ccttttttga cactatcacc aactgtaacg
    27181 aaggcaggtt tatctggtcc agcagccaag taaaccactc caacaagtgg actctctaca
    27241 agatttccct cactagccac acttgcttca gctggagctg gaacttcttc tgctacagtc
    27301 tctgctggag cagatgtagg agctactgga ctcggtgttg ctagaacggg tgctggagcg
    27361 acttgagttg caacttcagg cacaggtctc gcttcattct tgctaaactg caactcatcc
    27421 gtcccatttt tataagaaaa ttctctcaaa cttgactggt caaattgagt catcaagtct
    27481 ttaatatcgt ttaaattcat acttatctat tctcccaacg tttgaaagca agaactgcat
    27541 tgtggcctcc aaaaccaaaa gtatttgaaa tagcgtatgg aatttctttc tccaagcctt
    27601 gtccataaac gacattagct tcgatataat ctgatacttc acttgtccca gctgtcattg
    27661 gtacaaagtt atgacgcata gcttcgatgg tgacgatagc ttctactgca cccgcagccc
    27721 ccagcaaatg tcctgtaaaa gacttggttg atgatacagg tacttcctta ccaagaacag
    27781 ctacgatagc accactttct cctttttcat tggcaggagt tgacgttccg tgagcattga
    27841 cataggctac ttgctctgga gaaatctcag cttcttccaa ggctagtttg atggccttga
    27901 tagctccctg accttctgga tgtggagaag tcatgtggta ggcatcacaa gtatttccgt
    27961 aaccaaccac ttcagccagg atagtagctc cacgtttttc agcgtgttca agactttcta
    28021 gaaccaacat ccctgaacct tcacccataa caaacccatt gcgatcctta tcaaatggga
    28081 tcgaagcacg agttggatcc tctgtagtag agagagctgt taaggcttgg aaaccagcga
    28141 tggcaaaagg tgtgatagaa gcttctgttc ctcccaccaa catcacatct tggaaaccaa
    28201 acttaatgga gcggaaggca tccccaatcg catcatttga tgaagagcag gcagtattga
    28261 tagatttaca aacaccgttt gcaccaaaac gcatggctac attcccagaa gccatatttg
    28321 gtaaagcttt tggaagagtc attggtttga cacgtttggg tcctttttca tgaaggcgaa
    28381 gtacctgatc ttcaatttcc ttgattccac caataccaga tgcaacgata acaccaaaac
    28441 gatccctatt aagagcctct acatcaagat tggcatgatt tacagcctct tgggctgcat
    28501 acaaggcata taaagaatag ttatcaaaac ggttggtatc tttttttaca aagtatttat
    28561 cgaacggaaa atcttggatt tctgccgcat tatgcacatc aaagtcacta tgatcaaatt
    28621 ttgtaatgcc accaatgccg attttcccag ttgctaaact attccaaaat tcttctggtg
    28681 tatttccgat tggagatgtt actccataac ctgttaccac tactcgattt agtttcattc
    28741 ttttcacctc tagctttcgc tacatactta agccaccatc aatggcaacc acttgaccag
    28801 ttagataatc ttggcctgct aaaaatactg tcaaatctgc aacctgctct gcctgcccaa
    28861 attctttcat cggaatctga gctagtgtag cttccttaat cttatctgat aagatagctg
    28921 tcatatcaga ctcaatcatt cctggagcaa tcacattgac tcgtatattc cgactagcga
    28981 cctcgcgtgc cacagacttg gtaaagccaa tcaagccagc cttagaagca gcatagttag
    29041 cttgaccaat attccccatc aaaccaacaa cactagacat attaatgata gcaccttctc
    29101 tggctttcat catcggtttc aagactgatt gtgtcatatt aaaggcacca gtcagattga
    29161 ccttgagcac tttttcaaaa tctgcttctg tcatcttgag cataagagta tcttgggtaa
    29221 tccctgcatt gttgaccaaa acatctactg aacccagttc tgcaatagct tgatcaatca
    29281 tacgcttagc gtctgcaaaa tctgatacat ctcctgaaat gggaaccacc ttgataccat
    29341 agtttgaaaa ctcagcgagc aattcttctg agattgcccc acgactgttt aagacaatgt
    29401 tggctcctgc ttgagcaaac ttgtgggcga tggcaagacc aattccacga ctcgaacctg
    29461 taataaagat atttttatgt tctagtttca tttttttcct ttcaaaactt ctacttattt
    29521 tagtctattt ttctaaaagt gctactaaac tcgcttgatc ttccacatga gctaagtgag
    29581 cagtttgatc aattttttta acaaaacctg acaagacttt ccccggtcca atctcgataa
    29641 agttgcttat gcctgcttct tgcatgaccc caatactttc atagaaacga acgggttcct
    29701 tgacctgacg cgtcaagagc tgagcaatgt cctctttttg catcacagca gcttctgtat
    29761 tgccgactag gggacaagta aaatctgaaa aacttacctg agctagagtt tcagctagtt
    29821 tctggctagc aggctcaagg agagcggtgt gaaagggacc tgacacctta agaggaatca
    29881 agcgtttggc acctgcttct tgcaaaagtt caaccgctcg atcaactgca accacttctc
    29941 cagcaatgac gatttgtgca ggtgtgttat agttggctgg agtaaccact ccaagttcag
    30001 aagctttttg acaggcttct tcaatgacct ctactggcgt attgagaact gctaccatct
    30061 tgccagagtc agcaggagcc gcttcttcca tataggctcc acgcttagct accaaggcaa
    30121 ccgcatcttc aaaatccaag gcgccgcttg ccaccaaggc agagtattct ccaagagaca
    30181 aaccagcaac catatcaggc tgatagccct tttcttgcaa taaacggtag atagcaaccg
    30241 aagtcgctag aatggctggt tgcgtatagc gggtctgatt gagtttgtct tcttccgtat
    30301 cgatgagata acgtaaatca taaccgagca cctgactcgc tcgatcaatc gtttctttga
    30361 caatcggata ctgatcatag aaatcccgtc ccatccctag atactgggca ccttgaccag
    30421 caaataaaaa ggctgtttta gtcatttctt acaactcctg cccagcgaga ggcttcttct
    30481 tgaattttct tagcggctcc gtaatacaaa tcttttagga tttcttcagc tgtttcttct
    30541 ttagaaacaa gccctgcgat ttgacctgcc atgacagagc caccatccac atcaccgtga
    30601 acaactgctt tggctagggc acctgctccc atttgttcaa agatttctaa atcaggatct
    30661 tcctgcttaa aggcatcttt ttcagccagt tcaaaatctc tagtcaactg atttttaata
    30721 gcacgaacag catgaccaaa gtgctgagct gaaatcgtag tatcaatatc ccttgctttt
    30781 aaaattttct ccttgtagtt tggatgggca ttcgactctt ttgcaactac aaaccgtgtc
    30841 cccacctgta cagcctctgc acctagcata aagccagccg cagcaccttc accatccgca
    30901 attcctcctg cagcaataac aggaatagat atagctgtgg ctacctgtcg caccaaggtc
    30961 atggttgtta atttaccgat atgcccccca gcttccattc cttctgcaat aacagcgtct
    31021 gcaccgattt tttccatgcg tttagctaaa gcgacactag gaacaacagg aataacgatt
    31081 atcccagctt catggaaacg ttccatatac ttgcttggat ttcctgctcc tgttgtgaca
    31141 actttaacac cttcttcaat aacgagatcc acgatatctt ccacaaaggg agataagagc
    31201 atgatgttga ccccaaaggg tttatcagtc aatgatttga ttttatcaat attggccttg
    31261 acaacttctt tcggggcatt tcccccaccg ataattccta atcctccagc cttggaaaca
    31321 gcccctgcca aatcaccatc agcaacccag gccatccctc cttggaaaat aggatagtca
    31381 atcttcaata attctgtaat acgcgttttc atagtgcctc caaccttcct tgcttacgta
    31441 atagttcgat ttcaccataa tttgacagtc aaactattac ctaaacaaga gggagtgggt
    31501 ttctccctac tccttctact aatattctgc ttattttgct tgctcttcaa cgtaagcaac
    31561 caagtcacca actgttttca agtcattttc tgcttcgatt tggatatcaa aagcatcttc
    31621 gatttctgag attacttgga acaagtccaa tgaatctgcg tccaaatcat caaaagttga
    31681 ttcaagtgtt acttctgatg cgtcttttcc aagttcttca acgataattt cttgtacttt
    31741 ttcaaatact gccatgatag gactccttta aaataaatag tttttttata acaatgtgtt
    31801 caccacatga ttacctaaat tgtaagaatg agcgtgcccc aggtcaagcc tccaccgaag
    31861 cctgatagaa gaacagtctg gctaccatct aaagggatga gaccttgttc tacacactct
    31921 gaaagtaaaa tcgggatact ggctgcactg gtattgccat attccatcat attggctgga
    31981 agtttggctc ggtcaacacc aatttttcta gccatcttat ccaaaatacg gtcattggct
    32041 tgatgaagta gcagataatc caagtctgtc acctctatag gagattcatc aatagtctgc
    32101 ttgatagact tggctacatc tcgaatggca aaatcaaaga ctgtgcgtcc atccatcttc
    32161 aaaaacgaat ctgcactttc ttgatctgaa aatggagaat gcaaacctga atgcccataa
    32221 gttaaacact cgctgcgact tccatcgcta ttgagactct cagctaagaa atgctcttgc
    32281 tcgctagctt ctaacaagac accaccagca ccatctccaa acaacacagc tgttgatcga
    32341 tccgaccaat cgactgcctt agagagggtt tcactaccaa tcaccaagcc tttttgaaag
    32401 cgaccagaag cgataaactt ttcagcagtt gaaagagcaa atacaaatcc actgcaagcc
    32461 gcggttaagt caaaagcaaa ggctttatta gcgccaatat tagcttgaac acgagcagct
    32521 gtagagggca tcatcgaatc tggagtaatg gtagctagga tgataaaatc cagttctttt
    32581 cctgttattc cagcttttgc catcagtttc ttagcaacct ctgtagccaa atcactggta
    32641 gattctgttc ttgaaatatg cctttgtcgt attcccgttc gacttgaaat ccactcatca
    32701 ttggtatcca taatctgagc caagtcgtga tttgtaacca cttgctctgg cacataatga
    32761 gcaacctgac ttatttttgc aaaagccatt atttcaaatc ctccaaaaat tggtaaagat
    32821 tagtcaagcc tttacccatg acagcaattt cttcctcgct catgccatca ataatttttt
    32881 ctaccatggc cttgtggaag cgtttatgca gtctatgaat caagcgaccc ttctttgtca
    32941 aatgcagatg caccacacga cgatcctgtt ctgaccgaac tcgctcaatg tagcccttac
    33001 gctctaaatt gttcaaactt gtcgtaacag ttccaagagt taccatcaac tcttttgaca
    33061 cttgacttgg agtcacatct ggagccttac cgatgacatc aatcgtatgc atttctttga
    33121 tggagatatc cttaaaacga ctacctctca agttcacttc ctcaattaca aggacattgt
    33181 taaatataga tgttaaatat tcattgattc gttggtagtc catttttcat atccctcctt
    33241 cttcaaaaaa cttttacaat caaaacattt gacagaaaga attatatcaa aattcaaaga
    33301 atgatgcaag tattttttat tttcctgtaa atttaggtct tcttctctcc gaatgagctc
    33361 gcactccttc tttgaaatcc tctgtttgag ctaaggattt ctgtaggttc agttctaaag
    33421 tagcgtaacc ctgccagtct ttaaactggc tctcccaaac caacttctta atggcagcat
    33481 aggaattact tgaagcacgt cttaattttt taagaagctg ttctctcgtc ttttcaagtt
    33541 tttcagcttc agaaacgcgg taaaccaacc cccattctag ggctttttct gccgttaaag
    33601 cctctcctgt catagctaat tgagcagcac gcgtcacacc gatactgcgg ctcaagagat
    33661 gaattccccc tgcatctgga gccaaaccaa caccaacaaa ggcttggata aacttagcct
    33721 tatccgtcgc caaacagaaa tctgccgcaa cagccatatt cgctgcggca cctgcaacag
    33781 caccgtcaac ttccattaaa acaggtttgg ctatttgctt gattttataa gaaatcgtat
    33841 tgaccaactc tgcgattttt gtcaatgatg gaatatcatc ctcatccact gcccgcttca
    33901 tctctaccaa atctccccca actgagaaga cctttccatt ggcattgatt aagataaaat
    33961 gcacagctgg attttcttct gccaaagtca gagcttctaa aatctcctca cacatgggaa
    34021 tatgaaaacc atttgcgacc tcaggacggt tcaaggtaag gattgccaag tcctcttcaa
    34081 gctgataaat aatgtgttcc atcagggctc ctttttattt tataagcagt ttatactctt
    34141 cgaaaatctc ttcaaaccgc gtcagcttcc atctgtaacc tcaaaacagt attttgagca
    34201 gtctgcggct agcttcctag tttgctcttt gattttcatt gagtgttaaa ttatttcact
    34261 gtcaaagtat atctttttta ggagcagaaa gcaagaaaaa atcgactgaa agtgtctcag
    34321 ccgataagaa aggctcgctt taggctagaa agcatcttat tttcttcatt gaaaaaagcc
    34381 tttctttctg ctataatcgt ataaaatact tactttagga gttcttatga aagttgttaa
    34441 atttggaggt agttctcttg cctctgctag tcaattagaa aaagttttaa acatcgtcaa
    34501 aagcgattca gagcgtcgtt ttgtagtcgt ttctgcgcct ggtaaacgca atgctgaaga
    34561 tactaaggtt acggatgccc tgattaaata ctaccgcgac tatgttgcgg gtaacgatat
    34621 tagcaagagc caaagctgga ttatcgaccg ctatgctgct atggttagtg aattgggact
    34681 aaaaccagct gtgctagaaa aaatttctaa aagcattcac gccttggcca ctcttcctat
    34741 tgaagaaaat gaatttctct acgatacttt cctagcagcc ggtgaaaata acaatgccaa
    34801 attgattgct gcctacttta accaaaatgg tatcgatgca cgctatatgc accctagaga
    34861 agctgggatt gtggtcacaa gtgaacctgg tcacgctcgc atcattccat caagttatga
    34921 caagattgaa gaattgacaa acaccaatga agtccttgtc attcctggtt tctttggtgt
    34981 cactaaggaa aatcaaatct gtactttctc acgtggaggt tctgatatta caggttctat
    35041 cattgctgct ggtgtcaaag ctgacctcta tgaaaacttt acggacgttg atggtatctt
    35101 tgcagcccac cctggtatta tccaccaacc acactcgatt cctgagttga cctaccgtga
    35161 aatgcgcgag ttggcctatg caggcttctc agtccttcat gacgaggctc ttcttcctgc
    35221 ctaccgtgga aaaattcctc tggttatcaa gaataccaac aaccctgacc atccaggtac
    35281 tcgtatcgtt ctaaaacaca gtaatgatga atttccagtt gtgggaattg ctggtgactc
    35341 aggctttgtc agcattaaca tgtcgaaata cctcatgaac cgtgaggttg gatttggccg
    35401 caaggttctg caaatcctgg aagaacttaa catcggttgg gaacatatgc caacaggtat
    35461 cgacgatctt tctatcattc tccgttctcg ccaactaact cccatcaagg aagaagaaat
    35521 cctgcgtcag ttggttcaaa aggctaaagt agatcatgca gaaatcgaac acgacctttc
    35581 tatcattatg attgttggtg aaaaaatgaa gagccacatc ggagtgactg ctactgcgac
    35641 acgcgctcta tctgaaaaca aaatcaacat ccagatgatg tctcaaggtt ctagtgaagt
    35701 ttctattatg tttgttgtca ataaagacca agagaaagca gcaattaaag ccctctacaa
    35761 tgcctttttt ggagaaagta aggaagacta agcatggccc aatcacttaa caaaacagta
    35821 ctcctcagta cgacaggcac ttcctacctc tctatcgctg ggaaagttgg gaaattcctt
    35881 gtcggagatc aggctttgga attttaccca gatgtcaatg tcgaacaatt tatccagatt
    35941 ccttggagcc atatcaatca gattggagcc aatgtcactg gatgcaaaat cagccgccac
    36001 ttcgaagtct ttacagacaa aggaaaattc ctctttgctt caaaagactc aggtgccatt
    36061 ctcaaaattg cacgcgacaa attgggcaat gacaaggtcg tcaaacttcc gaccctgatt
    36121 cagacaatta gccaaaaatt taaaaatcta tttgcaaaaa aatagaaact ttagtataat
    36181 catagcaacg gataagtagg ttatcttctt ctttcagaaa gtctgcgggt gctgcgagca
    36241 gataggaggg ataggtgaaa ttctaccgtt agtaacaatt acatacacaa tcaagtgcac
    36301 acctgtgaag ttggatggaa ccgtggccct gccactccaa cgttttgtca ggtgtgcttt
    36361 tttcataaag gagttcttat gttagatatc aaacgtattc gtacagattt tgaagctgtc
    36421 gcagaaaaat tagctacacg tggtgtagat gctgctgtct tgaatgaaat gaaagaaatc
    36481 gatgctaaac gtcgtaacat cttggtcaag gttgaaactc tcaaagcaga acgtaacaca
    36541 gtttctgctg agattgccca agctaagcgc aacaaggaaa atacagatga caagattgct
    36601 gccatgcaaa atctatctgc tgaggttaaa gccttggatg ctgaattggc agaaatcgat
    36661 gctaaattga cagaatttac aacgactctt ccaaatatcc cagctgacag cgttcctgtt
    36721 ggggctgacg aagacgacaa tgtggaagtt cgccgttggg gtactccacg cgagtttgac
    36781 ttcgaaccta aagctcactg ggatctcggt gaagaccttg gtatccttga ctgggaacgc
    36841 ggtggtaagg taacaggcgc tcgcttcctc ttctataaag gcctcggtgc tcgtttggaa
    36901 cgtgctatct acaactttat gttggatgaa catggaaaag aaggctatac tgaagtcatc
    36961 acaccttaca tagtcaacca tgattctatg tttggtactg gtcagtatcc aaaatttaag
    37021 gaagatactt ttgaactcag cgataccaac tttgtcttga ttccaactgc tgaagttcct
    37081 ctggcaaact actaccgtga tgaaatctta gacggcaaag atcttccaat ctacttcact
    37141 gctatgagtc cgtcattccg ttctgaggct ggttctgccg gtcgtgatac gcgtggcttg
    37201 atccgtttgc accaattcca caaggttgaa atggtcaaat ttgccaaacc agaagaatct
    37261 tacgaagaat tggaaaaaat gacagccaac gctgaaaaca ttcttcaaaa actcaacctt
    37321 ccataccgtg tcgttgctct ctctactgga gatatgggct tctcagctgc gaagacttac
    37381 gacttggaag tgtggattcc agcacaaaac aattaccgtg aaatctcaag ctgttcaaac
    37441 acagaagatt tccaagcccg tcgtgcccaa atccgttacc gtgatgaagc agatggcaag
    37501 gtgaaactcc ttcatacctt gaacggttct ggacttgcag ttggacgtac agtggctgca
    37561 attcttgaaa attaccaaaa tgaagatggt tctgtgacca tcccagaagc acttcgtcca
    37621 tacatgggtg gagctgaagt catcaaacca taaaaaataa ggtttagcta tttctagcta
    37681 gacctttttt cgtaaccaaa tcagataagc acctagtaca aagaataaaa tagttaggca
    37741 tataatggtt tcagccaata ccaggtaatc cagaaatgga agtttcaaaa ttccctgagc
    37801 catcttgagc gaggtcgctg tgataatggt tgggaaggtg agggctgaga aggctggttg
    37861 aaaaccttgt tttaaaatgt tgggcagacg agttaaaaca aagaaaaaga aggattgaga
    37921 agccaaaatc atgacaatca agacccaatt cggcaggctg gttcctccta ctcgaactag
    37981 agaagccaag agtagagaga aaggagcaca gtagattcct tcttgtccaa gcaaggctag
    38041 tgggagtgga tgtttcttta aatcgctata aataagggga tagagataga aggtcaagag
    38101 aaaaccaaaa ctcaaggtcg cataggcaat ttcgataata cctaccagag gataggtcaa
    38161 ggcagccact gctatcccca catagagaac cgtccaactt ggagtggcat gaaccctccg
    38221 ccctgaacaa gcaaacttga tggtaaaacc agcaatcaag gtcaaatcca agagaaatga
    38281 aaaccaccaa atcccttgtg ctaccaaagg aagataagag aatacgcgaa agacataggt
    38341 cgataaaatc atcccagcca taggaaaggt tgccattcct gacaaaagag ggggcttggt
    38401 caattcttgc ttggtttctt tccaattaaa gagatgcaga attagaaagt aaatccataa
    38461 aaccaaacca gtcagactaa aaagatggga tagaaccggc aacgtatcta aaataagatt
    38521 tccagctcct gccaaaccta gcaaacaacc tgaaaatact aaggggagtt ttttcatcct
    38581 aacctccaat aatcatgtta gtttcagtat aacataaaag cgcttaaatg atgatttaaa
    38641 aaaacgaagt ccgattattt cagacttcat tttactcaga tatgaattag gcataaggtt
    38701 gcaattctgg attaattggt gtattagcta agttgttggc atagttacag aggattgcta
    38761 ggctgacacc aaaaaccaca tccaaggcat tttgttgagt gtagccagct tctaaaaact
    38821 cagacaaggc ttcatctcct acacgaccct tggtattgat aactgccaag gtaaacttag
    38881 ctagggtatc caatttagga tctgtttcaa ttggagtacg attgcgaaga gcttgaatca
    38941 agtcatcatt catctggatt tgtttgatgg aaaaggctgt gtgacctgcg acacagaagg
    39001 cacaaccatt ggtcacggct gccgtgattt gcaccacttc acgctcaacg ggtgtcaggc
    39061 tgttgcgacg gtggatagat gagacaattt ggtaggcttc taaaacagtc ggggcattgg
    39121 ccaagagacc gattaggttg ggaatatagc cattgttgtc tttttctact gtttcaagaa
    39181 tttctttcac ttctgctggt gctgactcta ctgtatggat agtaaatgtt gtcataagat
    39241 acctcttttc ttattattga cactaatatt attggaaaat cttataaaat cctgattcct
    39301 aagtttatct aagataaagc tttattctct cataagattt tcgttgttat attagtttat
    39361 cacacttcca atcacttgca taatatatat tatatatcag gctgataaaa attatttata
    39421 ggcaaaaaaa tcacacgagc tgtgtgattc cattatttgt caaaatactt tttagtttca
    39481 gcaataacga cttgcgacaa gaccaagagg gcaatcaagt ttggcagagc catcaaggcg
    39541 ttaacgatat ctgcgataat ccagaccata tccaactcga taaatcctcc taacaagacc
    39601 atgagcacaa aaaccacacg gtagagccag ataaagcgaa ccccaaagag gaactcaaaa
    39661 cagcgttctc cgtaatagtt ccaacctaga atcgttgtaa aggcaaaaag tacaaggaag
    39721 atggtcaaga gggcaggccc aaagtgtgaa aagactgttg agaaagctga ctgagtcaag
    39781 gcaaccccat tcaagtcacc actccaaact ccagttacca agatggtcaa accagttaga
    39841 gtacaaatga tgagggtatc aataaaggtt cctgtcatgg aaatcaaacc ttgctctact
    39901 ggttcatttg tcttggcagc tgcagctgca ataggagcag aacccagacc agattcgttt
    39961 gagaacacac cacgcgccac accattttga atagccatcc gaacgctagc accagcaaat
    40021 ccacctaccg cagcaagggg actaaaagct gaggtaaaga ctaaagcgat tgtgccaggg
    40081 atttttccga tattaaagaa aataactata agagttccta agatataaat gatggtcata
    40141 aaaggaacaa cagtagttga aaccttagaa atagacttga gtccaccaaa gactgcaatc
    40201 gctacaaaga cagacaagac gagagctgtg atgactggcg aaatcgtcgt tgtattttgg
    40261 atagattctg taatcgagtt gacttgggtg aaggttccga ttcccaagag agcaaccaat
    40321 actcctgcta ctgcaaacaa aacagcaagt ggtcgccact tttctcccat ccctagaagg
    40381 atatagtgca tgggacctcc cgctactgca ccatggtcgt ccttggtgcg gtatttgatg
    40441 gccaagagtc cttccgcata cttggtagcc attccaaaga aagccgccat ccacatccaa
    40501 aatagagctc ctggtccacc aaccttgata gccgtcgcaa ctcctatgat atttcctgtt
    40561 ccaacagttg atgccaaggc tgtacacaga gctgcaaaac tggatacatc accatgtccc
    40621 ttatcctgga taaaaataag ctgaaaggcc ttgggcagac gcaaaacctg caagagtcct
    40681 agccgcatgg ttaggtaaat ccctgttccg accaataaaa tcaagagggg cggtccccaa
    40741 gcaaaatcat cgattgattt aagcaattct aacatttcct tctcctatcg tttcaacccc
    40801 aaaagaaaga gcacatgcaa gatacatgta ctctggaatg cttagataaa tgctaaaaag
    40861 cggtctatcc tagctctgtc cttttacctg agagtttgag cagttgcctg ccttgcccct
    40921 tcggtgcctt tacggtctct ccagagttcc gtccatttac agtcatggaa aatcaaacga
    40981 ttccccactt ctattaaact tcattcggtg ttggtattta attgattcta attttacaaa
    41041 aaatgttggc ttttgtcaat gtgtttatta gtaaaaatta gttcaacagt ttttacttta
    41101 taaagtccag aatactgcta tcctttaaaa gtgacaatag tcgcaccact gcctccagca
    41161 ttttgtgggg catagccgaa actcttgaca tgtttgtttc tttgcaagta tttggtaact
    41221 ccttcacgga tgactcctgt tccgatacca tggatgatat caacttgagc catattgtta
    41281 agcaaggctt ggtcgatgaa ggtatctagc tcattcatgg cttcttcata gcgcttgcct
    41341 cgaagatcca gtctagcttg aggtcctcgc ccagaagttc gtttcacaac attgacctgt
    41401 ttcttcttga ctggtttttc ttgctgggct tgaacaagat caaactcttt ctcttccaag
    41461 gtcatcttaa tcaagccaac ttgggcttcc cagcggccgt ccttgagttg actggtcaag
    41521 gtaccacgct gaccataact gagaaccacg atatcatctc ccacctttgg agctcgtttt
    41581 ttcttggcct tttgaaggac cttgttttta gacaagtcca ctttttcagg agccaatttt
    41641 ttcaacttgg ccttggcttc aatgatttcg tggggcttga gttgggattt actgtggaga
    41701 tttttgagaa tctggtcact ttcacttagg gccatatcca caatctcagc agcctgttca
    41761 cgcgccttgt taagctcggt ttccttttca cgattaagct cgttgtagag tttttttagc
    41821 gcacggttca tcttgagatt ttcttgctcc acctcacgga tattgtccaa acgtttgcgg
    41881 ctttccagcg tctgctcttc taattgctca atgatacgat tgacgtcatt gtcctgatcg
    41941 atctgctgac tggcatctcc tacgataact tcagataggc ctagacgttt ggcaatttca
    42001 aaggcattac ttcggccagg aacaccctgc ataaagcgat aggtcgggcg aagagttgca
    42061 gtatcaaact ccatactggc attttgcaca aaggctgtct caataccgta ggccttgagt
    42121 tctggatagt gggtcgtcgc catggtcttg atttgacgca ggcgaaggtc ctccagaata
    42181 gccatggcaa gggctgctcc ctcttgggga tcagtaccag cccccaactc atccaaaagt
    42241 aagagtgaat gttggttgac cttgccaaga atatccacga tattggtcat atggctagag
    42301 aaggtagaca agctctgctc aatagactgc tcatctccaa tatcagcaaa gatttcttca
    42361 aaaataccaa cacgacttcc cttgtctgct aaaatcggca atcctgactg ggccatgacc
    42421 tgtgtcaagc ccagagtttt gagcatgatg gtcttcccac ctgtattggg acctgtaatg
    42481 acaatagctg ttaaatcttg accaaaatag acatcatttg cgacggcatt tttgaccaaa
    42541 ggatggcaga catggagcag ttgaatctct tgattttctg acagctgagg cacgactgct
    42601 tgtctttctt ggataaatcg aaccttggca cgaatcaagt ccagatgacc gataatccaa
    42661 gcgtcattag caatctcagc cgcatgaggg cggacacgct cagaaatttc ttggagaatg
    42721 cgaagcattt catagcgctc atctgctcgc agactagcaa tttcttcgct cagtttgact
    42781 acctcacgtg gttcgatata gacggtgttt ccactagcag aaatatcatg aacgacacct
    42841 gcaatcttat tgcggtaggt gtttttgact ggtaaaacct gacggccatt tctgctagca
    42901 acaattcctt ccgtcaacat ctgcgctttt tgcttgagta agtcttgtaa aacatcgcgc
    42961 acctgactct cgctatcatg tatttttcga cggattcgcg ccaattcttc actggcaaaa
    43021 ttttcaatga aacccgcatc attaaaggcc tgaagatttc cttgtaattg cggaaaatca
    43081 tgtaatttct caaaccaaag ggctaattct tccaagctga cattttccag attggcgtaa
    43141 aaattttgaa gttctcggct ggcaagaagc acgcgtttca agagtaggaa ctcctcgata
    43201 ttgagatccg ctcccatctc caacctcttg cagactcctg caatttcctt agttgagaga
    43261 atagtaaaat gcggttgctc gacgaaaaga gcctgcattt ctttcatctc agcaaaagcc
    43321 tgtttgattt tatctgcttt ggcagtcgga gccagctgtc tcaattgctc caagccctgc
    43381 tcggtcaaca aatgaggctc aaacaaggcc ttgaccttat cgaactctaa tgtttctaat
    43441 attttcttat tcatctttat ttccttgtct ttgaatgtct aggtgtggta gctttttaca
    43501 ttctgataga ttcgagtcaa atctgtaaac aaagggctag gaaaactcct gcccttttta
    43561 tccgattaaa tttgtcaccc agatttgttt gagccaactg gttgttaccg gtatgctctg
    43621 gatgatgtgt tttgcgacga tactcttttc aagaggattt tgtataactg ccatggggat
    43681 ggtcgccaag attgtcaagg ccatttgcaa gacaaataag gtcaccaaca tggacaagat
    43741 acctgctgaa acttggaaca acttaccacc cagttttttg ctaggaatca agtgtaagag
    43801 aagaccaagt aaacgaccaa tgctatagac aatcccaaat acaagcaagt agccgatacc
    43861 tgcataaaag accttatcca gctgaaagag ttgatccgat gggaaaaaga aagtcccctg
    43921 accttcctgc gaatttgcat aagggagcaa taaatggaat tgctctccaa gccctttata
    43981 aaactggcca gccataaaag ccgatgccat ggctgaaatc aggtaataaa cctgtaagag
    44041 caggcctctc cgatagccga tataaaatcc ccaaaccaag accaatagaa gaaggaatga
    44101 aatcataagg aatcctcaat cttgctctgt tcttgcttac aagtcacaag cttgtgacgg
    44161 agttcttcta gctcttgctc cttatcgtca aattcaatct cacggctgag ctgagttgat
    44221 aaacagttga ctgccaacaa aagagcgatt gtttcatcat ctgcgctagg catttgttct
    44281 ttaattgctt ggtatttttc tgtcgcaacc ttagcgattt cctccataaa aaggttgtca
    44341 tgttcgcttg tcaaggttaa cgattttttc ccgaatgtaa atttgaatcg atttagattt
    44401 gccataaaat tcacctcacg atattatacc aaaattcgct aattttgtca gtttttacaa
    44461 attttactgc ttttgtggta caatagaaac tatggcaagt atcacactca caccaagcga
    44521 aaaggatatt caggcttttc ttgaacacta tcaaaccagt ctggctccta gcaagaatcc
    44581 ctatatccgc tactttttga aactacctca agcaacggtt tctatctata cttctggaaa
    44641 aatcttgctt cagggtgaag gggctgaaaa atacgccagt ttctttggct atcaagctgt
    44701 agagcaaacc agcggacaaa atcttccttt aattgggaca gatgaggtgg gaaatggttc
    44761 ctactttggt gggcttgcag ttgtggctgc ctttgtcaca cctgaccagc acgacttttt
    44821 acgaaaactc ggtgtggggg attctaagac tctgaccgac caaaagatcc gtcagattgc
    44881 tcctattctc aaggaaaaaa ttcagcacca ggcactcctt ctctcaccca gcaagtacaa
    44941 cgaggtcatc ggagaccgct acaacgctgt ttcggttaag gttgccctcc ataatcaggc
    45001 tatctatctc ctccttcaaa aaggtgttca gcctgagaaa attgtgattg atgcctttac
    45061 cagtgctaaa aattatgaca agtacttggc acaagaggcc aatcgtttca gcaattctat
    45121 cagcttagaa gaaaaggctg agggcaaata cttggctgtc gcagtttctt ctgtcattgc
    45181 gcgtgatctc tttctggaaa atcttgaaaa tttgggacga gaactgggtt atcagcttcc
    45241 aagtggagct ggaacggctt ctgacaaggt ggctagccag attttgcaag cctatggtat
    45301 gcagggactc aacttctgcg ctaagctaca ctttaaaaat actgaaaaag cgaaaaacgc
    45361 ttagaaaggt aagttatgaa tttatttaaa aatttcttaa aagagtgggg attattcctc
    45421 ctgattctgt cattactagc tttgagccgt atcttttttt ggagcaatgt tcgcgtagaa
    45481 ggacattcca tggatccgac cctagcggat ggtgaaattc tcttcgttgt aaaacacctt
    45541 cctattgacc gttttgatat cgtggtggcc catgaggaag atgggaataa ggacatcgtc
    45601 aagcgcgtga ttggaatgcc tggcgacacc attcgttacg aaaatgataa actctacatc
    45661 aatgacaaag aaacggacga gccttatcta gcagactata tcaaacgctt caaggatgac
    45721 aaactccaaa gcacttactc aggcaagggc tttgaaggaa ataaaggaac tttctttaga
    45781 agtatcgctc aaaaagctca agccttcaca gttgatgtca actacaacac caactttagc
    45841 tttactgttc cagaaggaga ataccttctc ctcggagatg accgcttggt ttcgagcgac
    45901 agccgccacg taggtacctt caaagcaaaa gatatcacag gggaagctaa attccgctta
    45961 tggccaatca cccgtatcgg aacattttaa gaaacctaag aggccgagaa tcaccaatct
    46021 cagcctcttc ttctatcgtg agaaaatgat tggtaactat ctaaacttac cagaacagaa
    46081 acacctcaac tctcacctat tcatgcaaag gaattttatg gaagtttatt tttcaggaac
    46141 cattgaacgg attatttttg aaaatcccag caatttttat cgcatcctcc tcctagaaat
    46201 cgacgatacg gacgcagagg attttgatga ttttgaaatc attgtcacag gaaccatggc
    46261 tgatgtaatt gagggcgaag actatacttt ttgggggcaa attgtccagc actccaagta
    46321 tggagaacaa ctgcaaatca gtcgttatga tcgcgcaaaa ccaactagta agggcttggt
    46381 caagtacttt tcaagtagcc atttcaaggg gattggtctc aagacagctc agaaaatcgt
    46441 ggatacctat ggcgaaaata ccattgacga aattttgcaa cacccagaaa agttagaagg
    46501 catcgcagga ttctctgcca aaaatcgcga ggctttcgtc tccactctcc gtctcaacta
    46561 cggaacggag atggttttgt ccaaactagc caactacggc attcccaaca aactagcctt
    46621 tcagattcaa gacttttaca aggaagaaac ccttgatgtg gttgaaaatt atccctacca
    46681 gctggttgag gatatcaagg gtttgggctt tacaattgct gaccaactag ctgaggaact
    46741 aggcatcgaa agtcaggctc ctgaacgctt ccgcgccggt ctagttcaca gtctttttca
    46801 ggcctgtatg gaaacagggg acacctatgt tgaagcacgg gatttgctgg aacaaaccct
    46861 tactctcctt gagtcttccc gtcccgtgga actagacccc agccaagttg cccaagagct
    46921 ctcctacctg atcgaagaag acaaggttca gcagattgat accaagatct ttgacaacag
    46981 cctctttttc gctgaggaag gtatccgcag tcacttgatt cgtatccttg aaaaaggaaa
    47041 acagaagagc caagatttag aaactattca aaagcatatc actactgtcg agcaagagct
    47101 gggaattgag tatgataaca ttcaaaaaca ggctatctgt gatgctatcc agaataaggt
    47161 ctttatcctg acaggtggac ctggtactgg taagacaact gttatcaatg ggatcatcgc
    47221 tgtttacgcc ctgttagagg gacttgacct caggaagaaa agcaatctgc ccattcttct
    47281 tgctgctcca actggacgag ccgctcgccg catgaatgaa ttgacaggtt tgcctagcgc
    47341 gaccatacac cgccacttgg gaatgacagg tgacgatgat accagtcatc tggaagatta
    47401 cctagatgct gactttatca tcgtggatga attctctatg gtagatactt ggctggccaa
    47461 tcaactcttc tccaatatct cttctaacag taagatcctc atcgtaggtg acagtgatca
    47521 gctaccttct gtcagtcctg gacaggttct agcggatctg cttcatattc ctttgattcc
    47581 tcagactcgc ttggaaaaaa tttatcgaca aagcaaagaa tcaaccatcg tcaccctagc
    47641 tagtcagatt cgacagggca tcttgccagc tgatttcacc caaaaaaaag ctgaccgttc
    47701 ctactttgaa attgctagtg gccatattcc tgccaccatt gaaaaaatct taggtgtcgc
    47761 cctcagaaat ggtattcctg cccgtgatat ccaagttctg gctcctatgt accgagggac
    47821 ggcagggatt gatgctatca atcagctcat gcaagacctg ctcaatcccc cacaaaaaga
    47881 tcaactcagt tttgaagctc ctcagtgcca ctatcgtaag agagacaagg tcattcattt
    47941 ggttaacgat gctgaaatca atgtctttaa tggggattta ggagctatca cagacctgat
    48001 tcctggtaaa tacaccgaat cgaaacaaga cgagattgtc attgattttg atggtaatga
    48061 agtctcttac ccccgtaacg aatggtacaa gattcgcttg gcctatgcca tgagtattca
    48121 taagtctcag ggaagtgagt tcccagttgt catcctaccg attactagtg ctagccgacg
    48181 tatgctggag cgaaatctca tctacacagc cattacacgt gccaaaagca aactcatctt
    48241 actaggcgaa ttacaagcct tcgactatgc tacccaacac atcggaactg cccgaaaaac
    48301 ctatctgatt gaacgcttca gtgatctact ggagaatgtt gaagaaaagc aacaagctgt
    48361 ctctgaaaca gtcacatcaa gtgcctctga acaatcctac atcctaaccg aagaaaactg
    48421 ggaccgcatc ccagccatga ttgggattac agacacagac ctcaaagaga tttttggaaa
    48481 atagtgcatc agaaaaccca ccaattcagg tgggttttct gtttattaag attattttac
    48541 tgttgctgtg cttgtgatca attcaacagc ttttttgata gtgatatcat gtttcaacat
    48601 gtcagctgaa agcaagtttt gaacttgtgc aacttccatg ttgtagtctg ctgccaattg
    48661 ctcaacttct ttttggattt cttcttctga agcatcaaat ccttcagctt tggcaactgc
    48721 ttcgataaca aggttagtct tagtacgtga ctcagcttct gcttggtatt ggttgtgaag
    48781 gtcttcttga gtagttccag tgatttggaa gtacatgtca gggttgatcc cttgacgttg
    48841 caaattccca aggaattcat ttactgaacg gtgaacttct tcatggatca tttcttctgg
    48901 aagttctacg atttcagcat tttctacagc tgtatcaatt gctgcacctt caactgcatc
    48961 tttgtaagct tcttctttag cagcagccaa ttctttgcgg tatttttctt tcaagtcagc
    49021 aagtgtttca acttcttcat caatgtcttt tgcaagttca tcgtcaagag ctggaacttc
    49081 tttagctttt acttcgtgga tagttgtcac gaatttagct tctttacctg caaggtcttc
    49141 tgcttggtag tcttctggga atgttacgat aacatcaacg gtttcgccag ctgagtgacc
    49201 taccaattgg tcttcgaaac cagggatgaa ttgacctgaa ccaagtccaa gtgagaagtt
    49261 ttcacctttt ccgccgtcaa attcaacacc gtcgatagaa ccaacgaagt cgatcacaac
    49321 agtgtcgccg ttttcagcag cagcttcctt gataaccaat tcagccaggt tgttgcgttc
    49381 gcgttcgata cgctcttcaa catcagcgtc agttacttct ttttctacat caactgatac
    49441 ttcaaggttt ttgtagtcac ccaattttac ttcaggtttt gtaacgactt cagcagcgat
    49501 aacccagtct tgaccttttt ccattgaagt tacgtcaatt tttggttggg caaccacttc
    49561 aagaccagct tcttttacag ctgcttcata agcgtttggc aaaagagcgt tcataacgtc
    49621 ttggtaaagt gattcttcac caaatttttt atcgaagata ggacgtggaa ggtgaccttt
    49681 acggaaacct ggaacattaa gagatttctt cactgacttg aagacacggt ccaattctgg
    49741 tttgatttgg tcttgagaga tagtgaaagt caagacacca cggtttgttt ctttgttttc
    49801 aaatgataca gacattctgt catttctcct taaaattttt taaatacagt ctattataac
    49861 attttctata aataaaatct agactaatgc agccaattat agacaacaag cctttttcct
    49921 aactaaaaca ctcataaagt atagacaatt ttagactact ttggatatct tttggttagt
    49981 cattttccaa aactaattta tctaattttt cagttatatt tgacttcatc ttctcagtaa
    50041 catgagtata aacacttaaa atctttgttt cattagtata atctacacga tccattatcg
    50101 ctgttatagg tacttccaat tcagcaagca aacttatatg cgaatatcta aaaacatgag
    50161 cagtcaatac aaaatcatct ttcttaattt cacttttcct aaaactgttt ttcaaaaagt
    50221 aattgaaaca agttatcatt aagggagaac cattatttgt aaatatataa tcaccttctt
    50281 ctaaaagaag atattctttt aaaatttcta acactctatc actaatacca attactctat
    50341 atgaagctaa acttttcgga ctatccaaaa atacttcgtt attggacgat taca
//
LOCUS       DAVBDG010000007        46991 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBDG010000007 DAVBDG010000000
VERSION     DAVBDG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.007"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(103..1293)
                     /locus_tag="VQ526_000371"
     CDS             complement(103..1293)
                     /locus_tag="VQ526_000371"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2554143.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKAKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(1670..3013)
                     /locus_tag="VQ526_000372"
     CDS             complement(1670..3013)
                     /locus_tag="VQ526_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2554144.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIADSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQSMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(3153..3968)
                     /locus_tag="VQ526_000373"
     CDS             complement(3153..3968)
                     /locus_tag="VQ526_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2554145.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            complement(4020..6200)
                     /locus_tag="VQ526_000374"
     CDS             complement(4020..6200)
                     /locus_tag="VQ526_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2554146.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            complement(6486..7412)
                     /locus_tag="VQ526_000375"
     CDS             complement(6486..7412)
                     /locus_tag="VQ526_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554147.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            complement(7405..8097)
                     /gene="ftsE"
                     /locus_tag="VQ526_000376"
     CDS             complement(7405..8097)
                     /gene="ftsE"
                     /locus_tag="VQ526_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2554148.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            complement(8115..9210)
                     /gene="prfB"
                     /locus_tag="VQ526_000377"
     CDS             complement(join(8115..9137,9139..9210))
                     /gene="prfB"
                     /locus_tag="VQ526_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2554149.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(9318..9974)
                     /locus_tag="VQ526_000378"
     CDS             complement(9318..9974)
                     /locus_tag="VQ526_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2554150.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            complement(10282..10992)
                     /locus_tag="VQ526_000379"
     CDS             complement(10282..10992)
                     /locus_tag="VQ526_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554151.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            complement(10992..11756)
                     /locus_tag="VQ526_000380"
     CDS             complement(10992..11756)
                     /locus_tag="VQ526_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554152.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            complement(11756..12712)
                     /locus_tag="VQ526_000381"
     CDS             complement(11756..12712)
                     /locus_tag="VQ526_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2554153.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            complement(12716..13585)
                     /locus_tag="VQ526_000382"
     CDS             complement(12716..13585)
                     /locus_tag="VQ526_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2554154.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            complement(13853..15013)
                     /locus_tag="VQ526_000383"
     CDS             complement(13853..15013)
                     /locus_tag="VQ526_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2554155.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEVAEVVKP"
     gene            complement(15114..15362)
                     /locus_tag="VQ526_000384"
     CDS             complement(15114..15362)
                     /locus_tag="VQ526_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2554156.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(15441..16031)
                     /gene="clpP"
                     /locus_tag="VQ526_000385"
     CDS             complement(15441..16031)
                     /gene="clpP"
                     /locus_tag="VQ526_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2554157.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAHETLEYGFIDEIMANNSLN"
     gene            complement(16205..16834)
                     /gene="upp"
                     /locus_tag="VQ526_000386"
     CDS             complement(16205..16834)
                     /gene="upp"
                     /locus_tag="VQ526_000386"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2554158.1"
                     /translation="MGKIEVINHPLIQHKLSILRRIDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(16923..17390)
                     /locus_tag="VQ526_000387"
     CDS             complement(16923..17390)
                     /locus_tag="VQ526_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2554159.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(17409..17966)
                     /locus_tag="VQ526_000388"
     CDS             complement(17409..17966)
                     /locus_tag="VQ526_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2554160.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            18091..18936
                     /locus_tag="VQ526_000389"
     CDS             18091..18936
                     /locus_tag="VQ526_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2554161.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            18995..20170
                     /locus_tag="VQ526_000390"
     CDS             18995..20170
                     /locus_tag="VQ526_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2554162.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDNQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDEVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            20190..20645
                     /locus_tag="VQ526_000391"
     CDS             20190..20645
                     /locus_tag="VQ526_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2554163.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(20674..21414)
                     /locus_tag="VQ526_000392"
     CDS             complement(20674..21414)
                     /locus_tag="VQ526_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2554164.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSETAQYTSDAIAAYVQTNAE"
     gene            21519..23023
                     /locus_tag="VQ526_000393"
                     /pseudo
     CDS             21519..23023
                     /locus_tag="VQ526_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(23014..23958)
                     /gene="manA"
                     /locus_tag="VQ526_000394"
     CDS             complement(23014..23958)
                     /gene="manA"
                     /locus_tag="VQ526_000394"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2554165.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(24090..24371)
                     /locus_tag="VQ526_000395"
     CDS             complement(24090..24371)
                     /locus_tag="VQ526_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2554166.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGIDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            24436..25482
                     /locus_tag="VQ526_000396"
                     /pseudo
     CDS             24436..25482
                     /locus_tag="VQ526_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(25599..25946)
                     /locus_tag="VQ526_000397"
     CDS             complement(25599..25946)
                     /locus_tag="VQ526_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2554167.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(26057..27832)
                     /gene="spxB"
                     /locus_tag="VQ526_000398"
     CDS             complement(26057..27832)
                     /gene="spxB"
                     /locus_tag="VQ526_000398"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2554168.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(28039..30282)
                     /locus_tag="VQ526_000399"
     CDS             complement(28039..30282)
                     /locus_tag="VQ526_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2554169.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLVISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(30292..30663)
                     /locus_tag="VQ526_000400"
     CDS             complement(30292..30663)
                     /locus_tag="VQ526_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2554170.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            complement(30674..31069)
                     /locus_tag="VQ526_000401"
     CDS             complement(30674..31069)
                     /locus_tag="VQ526_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2554171.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      31253..31351
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            31435..32226
                     /gene="thiD"
                     /locus_tag="VQ526_000402"
     CDS             31435..32226
                     /gene="thiD"
                     /locus_tag="VQ526_000402"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2554172.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(32435..33067)
                     /locus_tag="VQ526_000403"
     CDS             complement(32435..33067)
                     /locus_tag="VQ526_000403"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2554173.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(33060..33863)
                     /locus_tag="VQ526_000404"
     CDS             complement(33060..33863)
                     /locus_tag="VQ526_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2554174.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(33863..34387)
                     /gene="thiW"
                     /locus_tag="VQ526_000405"
     CDS             complement(33863..34387)
                     /gene="thiW"
                     /locus_tag="VQ526_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2554175.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            complement(34392..35084)
                     /gene="tenA"
                     /locus_tag="VQ526_000406"
     CDS             complement(34392..35084)
                     /gene="tenA"
                     /locus_tag="VQ526_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2554176.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(35095..35745)
                     /locus_tag="VQ526_000407"
     CDS             complement(35095..35745)
                     /locus_tag="VQ526_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2554177.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(35747..37132)
                     /locus_tag="VQ526_000408"
     CDS             complement(35747..37132)
                     /locus_tag="VQ526_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554178.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILLQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREINQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(37133..37693)
                     /locus_tag="VQ526_000409"
     CDS             complement(37133..37693)
                     /locus_tag="VQ526_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2554179.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(37955..38043)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(38252..38881)
                     /locus_tag="VQ526_000410"
     CDS             complement(38252..38881)
                     /locus_tag="VQ526_000410"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2554180.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(38883..39665)
                     /gene="thiM"
                     /locus_tag="VQ526_000411"
     CDS             complement(38883..39665)
                     /gene="thiM"
                     /locus_tag="VQ526_000411"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2554181.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKKFKRVK"
     gene            complement(39947..40615)
                     /locus_tag="VQ526_000412"
     CDS             complement(39947..40615)
                     /locus_tag="VQ526_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2554182.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENNYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(40830..40918)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41172..42308)
                     /gene="lctO"
                     /locus_tag="VQ526_000413"
     CDS             complement(41172..42308)
                     /gene="lctO"
                     /locus_tag="VQ526_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2554183.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(42934..44424)
                     /gene="lysS"
                     /locus_tag="VQ526_000414"
     CDS             complement(42934..44424)
                     /gene="lysS"
                     /locus_tag="VQ526_000414"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2554184.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLKEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(44552..44698)
                     /locus_tag="VQ526_000415"
     CDS             complement(44552..44698)
                     /locus_tag="VQ526_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET2554185.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            44925..45593
                     /locus_tag="VQ526_000416"
     CDS             44925..45593
                     /locus_tag="VQ526_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2554186.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRIPV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPLIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVVLPSFSANVIFLIKKTSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTCHAGFGNPST
                     LSRK"
     gene            45553..45933
                     /locus_tag="VQ526_000417"
     CDS             45553..45933
                     /locus_tag="VQ526_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET2554187.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            45943..46230
                     /locus_tag="VQ526_000418"
     CDS             45943..46230
                     /locus_tag="VQ526_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2554188.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            46231..46991
                     /locus_tag="VQ526_000419"
                     /pseudo
     CDS             46231..46991
                     /locus_tag="VQ526_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
BASE COUNT        13799 a        10254 c         8763 g        14175 t
ORIGIN      
        1 attatacatg taaatctcta ctttcgcaaa atggattttc agaacagtat cccagtatag
       61 ttaaaaacta taaaaaagag aggacgttcc cctcttaaaa tcttatttta ctgcttcttt
      121 caaagcatct accttatcca aacgttccca tggaagatca atatctgtac gtcccatgtg
      181 accgtaagcc gatgtttgac ggtaaattgg acgcttgagg tccagcattt ggataatccc
      241 tgcagggcga aggtcaaaga tttgacgagc cgctttttca agttgacttt cagctactgt
      301 tcctgtaccg aaagtatcga tacgaacaga aacaggttgc gcaacaccga tagcataggc
      361 caactgcact tctgccttct tagcaaggcc tgctgcaacg atattcttgg caatatagcg
      421 agccgcataa gaggctgaac gatccacctt agtcgcatct ttaccagaga aggcaccacc
      481 accatgacga gagtagccac cataagtatc tacaataatc ttacgaccag tcaaacctga
      541 gtccccttga ggaccaccga ttacaaaacg acctgtcgga ttgataaaga attttgcctt
      601 atcatcaaga taagaagatg gaataacttc tttgatgacc ttgtcaatca catcttgatg
      661 gatttgttca ttagtggcct ctggatcatg ctgagtagaa ataacgactg tatctacacg
      721 taccggacgg tcattttcat cgtactcaac tgtaacttgt gattttgcat ctggacggag
      781 atagctaatt tctccagact tacgaagttc tgccagacga cgaaccaatt tatgactgag
      841 tgcaattggc aatggcatga gctcttctgt ttcatctact gcaaatccaa acatgagccc
      901 ttggtcacct gctccaatca agtccagtgg atcttgatca gcatttccac gaacctccaa
      961 ggcttcgtta acaccttgag cgatgtcagg agattgttcc accaaagatg ggtgtactcc
     1021 caccgtctca gcagaaaatc catattctgt attggtataa ccaatctctg caatggtatc
     1081 acgaaccaca cggttaatat ccacataggc atttgtagaa atttcaccaa aaacgtggac
     1141 agaaccagta tatacagctg tttcagcagc aacgtgcgcc tctggatcct ttgctaaaat
     1201 agcatccaaa atcgcatctg aaatttggtc tgcaatctta tccggatgcc cctcagatac
     1261 agattcagac gtgaataatt tacgttctga cataaaaatg tcccccctta aaaaatattg
     1321 ttatagagtt acaaagtact gataggaatt tccattatct aagagttact agcttcagaa
     1381 ataaaaacga tactttttta tttctgaagc tagtaaggcg catagggtga ccgcagtatg
     1441 gcggcaaccg tagaaagacg agcgacctcg aagccgtcat tctttgcgaa ttgcaaacta
     1501 ctgataggaa aactcttcgt aataaatacc taccatctta tcgggactat ataactttat
     1561 cattatacca tttttaatcg caatttgcag tctttaatgt agtgtttcgg tttagatagt
     1621 tcgttctcat aaataaaaaa gttggagctc agtactccaa caatttctat taaaatgttt
     1681 ggcgtttagc tttacgctct gcacgaccgc gagcgcgatt ttcagcacgc ttggttttac
     1741 ggcgcttttc atcaaccgcc cattgaattt tcttcttata acccggtttg acttttttct
     1801 ttttcttttt aaccaaacca atcatttcga tatcaagttt atcttgtttt ttctcacggt
     1861 tggcacgacg atcacggtca taggtatctt gaaattcccc gtctttgacc atcttaggac
     1921 taaacttgat tcccaatttc tccaactcac ggatatccga gtcatcactt ggctgataaa
     1981 gggtaatagc tgtacctggt aggccatttc gtccagtacg accaacacgg tgaacaaaaa
     2041 aagataagtc ttgcggaatg gcatcattga tgacatggct gacaccttca atgtcaatcc
     2101 cacgtgctgc caaatctgtt gcgacaatat actcaaaatc cagattttgc acctgattca
     2161 tgattcgctt gcgttcacga ggggcaatat cgccatggat ttttgcaacc ttcaagcctt
     2221 gagcagtcag atatgaatgc aattcatcag cacgcgtttt agtgttaaca aaaatcattg
     2281 ccaaatacgg ctgcatcgac tgagtcaact ggtaaatttg agcattctta tcatgtccct
     2341 tggtcgaaat caaccaatta tcaatggtgt cagaaataac cgttttggtc ttaattttct
     2401 ccataacagg atttgataag tattttttca agaatggttg cagtttttgt gggatagtcg
     2461 ctgagaagac catgaattgc aagtcttttg gaagactgtc agcaatctta tcaacagttt
     2521 ccaagaatcc catatccaag gtcatatctg cttcatcaac aacaaatgtc ttggctttat
     2581 gaatagctaa atcaccagat ttaaccaagt cgtagatacg gcctggtgtt ccaataacaa
     2641 tatgaggctg attgcttgcc aatttctcaa tctggcgagc cttatccgta ccacccacat
     2701 aattaaccac acgaacttcg acatctgagt gagctgaaat ctgacgcgct acttggtaaa
     2761 tttgagtagc caactcacga ctcggtgcag taatcactgc ttgtacacta tcgctagctt
     2821 catctaattg ctgaaaaatc ggtaacaaga aagtatgagt cttacctgaa cctgtttttg
     2881 attctcctac taggtcacga cctgccaaaa caataggaat caacttgtct tgcacctctg
     2941 ttggagttgt aaattttaac tccttcaagg cttctctaat atagttttta aattgaaatt
     3001 tcgtaaatga cataacatcc tcgattctat ctatcttatc aattatacca tattttattc
     3061 cattacagta gtctcactta tttaggctat ttccagtagc ttctctagta agaaaaggct
     3121 ggaattttat agttccaacc tcttttcagt tattatttcc agtttaatat agcattcaag
     3181 ccatagtgat cactcacttg tggactcttg ttaccatcaa atacgacatg taaattttcc
     3241 accgctaact ctttggtagt aaagacataa tcgattcgaa ggggttcagt gttccctttc
     3301 cagccatcaa tttcaggcgg aacagtatag ctaccacttt tctcttgagc aacttcaaat
     3361 gcgtcttgta agcctaatgg actagctaaa atagcttggt aaccttcctg acctgctggg
     3421 ttgttaaaat ctccagctag taaaagtggc ttgttcaatt ttttcaagac agcctcaaat
     3481 cgtgcccatt cttcttgaaa acctttatcc caccaagaga gatgaacact ggcaactgct
     3541 agctccttgc catcgactac agtttcagct agggcaacac ggcgagtatg atagtctgtt
     3601 ggatcatcca catctgaaac caaaatttct ctggcttcaa taggtgtttt agacaagata
     3661 gccacacctt cgtggtagcg gtcatagccg atatggttat aagcccaggt ccagtagtaa
     3721 tttttccctt gctctaacaa cttttcaacc aagagtctaa cataatggtc ttggtgaata
     3781 ggctcagctg ctggcaaagc ttgataaagg tcattaacct ccacctctga cgaggtcatc
     3841 tcctgattga tttcttggaa acaaatcaaa tcatagtcct tttcaagaat atcttcaagc
     3901 aaaatctgga atttttcctc tgcttctttc tccatccaac tgtgagtatt gagtgttaaa
     3961 aatttcattc ttttctccca aaataagagg ttggaataca gcttccaacc tcaagtatat
     4021 tacaattcta ctttagcaac tgctgtttta gctgcaagag aacctgtttt ttctaattta
     4081 actgatttaa ttgcatcacc atttgtgaag acaactactg ttgaagtttc acgtcctgct
     4141 gcacggatag catccaagtc agctgtgaca aggagatctc ctgctgcaac tttttgtcct
     4201 tcagcaacat gaactgtaaa tggtttacct tcaagactta ctgtgtccaa accaatgtga
     4261 accaatactt caagacctgc ttccgtcaca ataccaaaag catgttttgt tgggaagatg
     4321 cttgacacag tacctgaaac tggagataca atgtttccat ttgcaggttc tactgcaaat
     4381 ccatcaccca tcattttttg agcaaatact ggatccttta cttgttccaa accaacaact
     4441 tgaccgtctg ctactgagta aacttcctca gtaagatttt tgaagtgaac agtgttttgt
     4501 tgtgcttcag tcatttggct tggaagagtt tcaggaatga tttcacctga atcaaggata
     4561 tcttggatat cagatttcaa aatgtcagct tttggaccgt agatagcttg aaccccttgt
     4621 cctttcatca caagacccat agctccttct gctttccatt gctctgcatt tcctacttta
     4681 tctgcatctt taacagttac acgaagacga gtcatacatg catcaacatc aacgatgttt
     4741 acacgtccac caagaaggtt gataatgttt acagcttgag agcctgctgc aactttcact
     4801 tcgctgctgg tttcttctga accttcagca gtttcgtagt ttccgttgcg ccctggagtt
     4861 gcgtagttga atttttgaat catgaagttt gcgataaagt acatgattac agcaaagaga
     4921 acagttaccc aaacgaagtt aacgatatcc ataccaatac cagcactgat tgcaataggt
     4981 gtacgagtca agaactcgat tgaaccgaat gagtgcatac gtaggtttac gacgtcagcc
     5041 atagcgaagg cagcaccttg aacaagtgag taaacaagat acataggtgt tgcgatgaac
     5101 atgaacatgt attcgattgg ttcagtaacc cctgtcaaga atgttgcaag agctgttgca
     5161 atcatcatac ctttgtattt atgtttcttg tcagcatcaa cattacggta gatagcaaca
     5221 atcacaccca tcaagatacc gaatgaaccg atcatttgtc caactttgaa acgagctgga
     5281 tgtactgtat ctaacaagtg ttgatattga ctagtatcag tacctttaag gtttacaagg
     5341 tctgttaccc atgcaagcca tagtgggtct tgaccgaata cttgagtacc tttagctgca
     5401 ccagttaaaa tgtcataagt accaccaaga gctgtgtagt tcattgggat agtcaacatg
     5461 tggtgaagac caaatggcaa gagcaaacgt tccaaagtac catacaagaa tggtgcaaga
     5521 attggagcag tttcttgtga gttggcaatc cagataccga agttattgat acctgtttga
     5581 actactggcc agaaagcagc aagtagaatt gcagcgattg ctgaacgaag aataactaca
     5641 aatggtacga aacgtttccc gttgaagaat gaaagtgcat caggaagttt acggaagttg
     5701 tagtatttgt tgtaagcagt tgcccctaca aaacctgaga taatccctac gaataccccc
     5761 atgttcaagg ctggagcttc aagaacactg ataaagtaat cagcaacttt gattgaacca
     5821 ccaaagaaag tagttaccat agcatctgga tttttcaaca tatcgcctga tacaccaaaa
     5881 attgtaccag tgatacggtt aatcaagatg aaggcaagac cagcggcgaa agcaccacca
     5941 gcacgttctt tagcccagct tcctccaatg gctagggcaa acaaaatgtg aaggttaccg
     6001 ataacccccc aaccgatttg ctcaagaatt ccacctgtga tgacaagtgg tgtaaaggtt
     6061 gggttaatca tcacgataga cttaccgatt gaaatcatca aaccagcagc cggcataacc
     6121 gcgataacta ccatcaaagc cttaccgaat ttttgccaaa attcgaaaga caagacattt
     6181 ttgaatgtat ctttcatcat tcaaatctcc tttattttta tttaggcaaa cgttttacga
     6241 aaacgtttgc accttctatg atttaattat accactttaa ttttttttgt aaaggctttt
     6301 ataaaaaaat tacacttttt tctaaaaaat ttctagtgcg ccaaatttca agttcaaatt
     6361 agccatcaag aagtcttctc tgggggactt ctttggaagc tcttttcttc tacgcatctc
     6421 ttttcaaaaa ctcttgtagc aaaaaaggag attttacaat ctcctcataa aagcagctat
     6481 tttacctaaa tcttcaagaa tcggcgcatg gatattcctg atcccaatga accaatgaaa
     6541 accccaatca caaatagtag ggcaatcatc aacggactaa ataaatctgg actaatcatg
     6601 gatagatttt gccctaccaa cgatttgttg acagattggt aaacaatttg ataaacaata
     6661 aagaccaaaa cagatggtgc gatagctccc aataaaccga taaaggctcc ttctaacaag
     6721 aacggtccac ggatataact gtttttagct ccaaccaagc gcatgatttg aatttcgcga
     6781 ctgcgggaaa taatggtaat acgaatggta tttgaaatca agaaaactgc gataaaaatt
     6841 aacaaagcag caatccctag tccccaaaca cggataaatg aagctaactt gaagagtctt
     6901 tctgtattgg caccgccatc ttgaacctca gagacacctt caattttttt agcatcttcg
     6961 gctatagttt ttacatcatt tggagtgttt gcctctacaa tataggcatc atagagagga
     7021 ttggcatctc cttcaaagat tttccagtta tctcccatta tctcggttaa tttttcatat
     7081 tgttcttctt tacttgaaaa ggtaacactt ttaaccgtag acatgttctt caaagaatca
     7141 tataccttgt ggtagtcatt atttgtaaca gtttgacctt ctttttcaat tgtctgacta
     7201 ttatcttcca catcctttcg gatataaact actacacgga cattattttc aatatctgta
     7261 gctaatttcg ctgtattgaa aataacagat gcaaatattg ccaccaaggt caaagtaatc
     7321 atgactgaac tgacagcagc tactgtcatc caaccatttc gtttcaaact ttttaaggct
     7381 tcaaataaat ggcgaaaaaa tctactaatc atcgtatcca tactctcctt ttgattcgtc
     7441 acgaacgaca cggccatttt caatggcaat gacacggtgg cgcaaggtat ttacaatctg
     7501 gctattatga gtcgccatca aaatagttgt cccttgtagg ttaatccgtt ccaagagatt
     7561 cataatttcc catgaattat ccggatccag atttcctgtt ggctcatcag ctatcaatac
     7621 tttgggatta tttacaattg cacgcgcaat cgcaattcgc tgctgctccc cacctgagag
     7681 ttcatttggg aaagaacgaa ccttatgctt caatccaacc aagtccaaaa cttccatcac
     7741 tcgtcttttg atattacggc gattttcccc gattacttcc atagcgtaag caatattttc
     7801 atagacagtt ttctttggta acaatttata atcttggaag acaaccccaa cactacgacg
     7861 tagaagcggg acatctttct ttttgatctt aaccagatta aaaccagcaa ctgatagact
     7921 tcctttatcg atttttactt cacgatacag agaacgaata aaagttgact tccctgctcc
     7981 tgaaggtcct acgatgtaag caaattcccc cggttgaacg ctaaccgaaa caccgcgtag
     8041 agcagtcgtt ccgttgtcgt attttttaac gacatctctc atttcaataa ttgacatgtg
     8101 agttcctttc tatcttagct aattcgccac ttgagataag catcgataaa accatctagg
     8161 tccccatcca taaccttatc tacctgagca acctcaaagc tagttcggtg atcttttacc
     8221 atagtataag gcgtgaagac ataagaacgg atttggcttc cccaagtgat ctcctttttc
     8281 tcacctttga gagaatctac ctccgcagcc ttcttatctt gctccatttg atagagctta
     8341 gcctgcaaca tcttcatggc acgatctcta tttccatact gggtacgatc cactgttgat
     8401 tggacaacaa ttccagttgg aatgtgggtt aaacgtacac ctgttgaaac cttattgacg
     8461 ttttgtccac cggcaccacc tgaacggaag gtatccatct tgatatcatc ttcacggatt
     8521 tccacttcaa tagtatcatc caattctggc atcacttcta cagatgtgaa agaggtatgg
     8581 cgacgtttgg cagagtcaaa tggtgagatt cgcactaagc gatgaacacc catttctgac
     8641 ttgaggagac cataggcatt aggcccttca aatgataaag ttaccgactt aataccagcc
     8701 tcatcacctg cttggtaatc caacacttcc actttaaagc ctttagcatt accataacga
     8761 gtatacatac gaagcaacat atcaccccag tcctgcgcct cagtaccacc agaacctgga
     8821 tggatttcca agatggcatt gttgtggtca taaggttctg acaatagtag agtcatctcg
     8881 tagctggtca ttatcttatc aagttcggct aactgcgcta ccagttcatc atgcactgac
     8941 tcgtcttcag ccaaaaaatc caataaaatt tcgacttcat cctgcaactc ttccatctta
     9001 tggaaggtat tgtaagtgtt ttttaattca ttcaattctt gcgacgtttt ttgggccgca
     9061 atattatcgt tccaaaaatc aggttctgtc atcttgtttt ccaagatggc aatctcttcc
     9121 tctagccctt cgaggtcaaa gagaccccct gaaagaagct aatttttcac gatttgcgtc
     9181 aattttctga cgaattactg aaatgtccat aaatactcct tttttacatt atttcattat
     9241 agcatatttt atcatttctt tccatctttt gctctaatcc ccttttcatg aaaaaagaag
     9301 cctttgggct tcccttctta caagactttt gctgaagtgc gagcgatttc ttccacttga
     9361 ataccatttg cttcaaattt ttctttcaag gctggtaaat caattgatcc atcgatttgc
     9421 acttcgataa tcaccttacc atccttacgc ggaatattga ctgtatggga gatattcaaa
     9481 ttttcttcta caatcaaaga aacaattttt ccaagaacac caacttcatc ttctgtaaca
     9541 aagcgtacac gaatcccttc ttcgccataa cctgcaattt caagaaaggc ttggaaaacg
     9601 tcacggtcag taataactcc gtatacttga tggttatcta cgacagggag aatactaatc
     9661 ttatttttca acatcagata agttgcatct tctagactag catagcctga gacagtgaca
     9721 acatcgcgaa tcatgacatc ttttactttt gtcttattca gaagataatt catctcatag
     9781 atagaaagac ttgttgcttt agatggactt gcttgtgcaa tggttccctc agtcaccaaa
     9841 ccaactaatt gatcattttc gataacaggc agacggtgca aaccttgctc tctcatcaaa
     9901 tctgctgcat gagatactgt tgtatctgga ctaatataaa ctaccttgcg ggtcataaaa
     9961 tctttaactg ccatgagact tctccttttc tattcttatc cctattatac aatctttttg
    10021 taaatctatc aaacgcttac atttcttttt cgaaattcag aaaagtatga ataagctagc
    10081 aaaaagagct ctgaaataga gctctgtgaa tgaaggtaag aaatttttca ttcgattttt
    10141 tcaattatta gaaattaagc tttcaatgta gatgcatatt tgtttgcaat ctcaatctac
    10201 cgactaaaaa ggtcccccgg actatattga tgattacttc gtaatcttca tctgccgact
    10261 aaaacaatcc actggattgt tttagccacc tagatatgct tttctgactt cttctgatga
    10321 agcgagttct tttcctgtcc ctgataggac gatttttcct gtttccaata catatcctcg
    10381 gtcagagatt gcgagtgctt tattggcatt ttgttcaatc aagaggacgg ttgttccttg
    10441 cttctgaata tcttgaatga tatcaaaaat ctcttggatg aagattgggg caagtcccat
    10501 tgatggttca tctaaaagaa gaagttttgg tgttgacata agagcgcgtc ccatggcaag
    10561 catttgttgt tcccctcctg aaagagtagc tgcatcttgg ttcttacgtt cttcaagacg
    10621 aggaaagcgt gagaaaacct tcttcaagtt tgcttgattt tcttcacgat ttttctttaa
    10681 gaaagctccc atttcaagat tttccataac agtcaagcca ggaaagacgt ggcgtccttc
    10741 tggaacttgt gaaagaccac ctgccacgat tttctgagct ggcatttttt ggatttcttg
    10801 acctaaaaat tcaatctttc ctgaacttgg tcgaaccaaa cctgacaagg tgcgaagaat
    10861 agttgtctta cctgcaccgt tggcaccgat aagggaaaca acttctcctt cattaacttc
    10921 aaagcttaca tcacggactg cttggatcat accgtaatgc acagaaagat tttcaacttt
    10981 taacatagac attaggcttc acctcctaga taagcttcga taacgcgttt attggtctta
    11041 atttcgtctg gagttccttg agcaattaaa cggccatatt caagtacgta gatacgttct
    11101 gttacttcca tgaccagatt catatcgtgt tcaatcaaca tgattgtaat cttaaattca
    11161 tctttgatac gacgaattaa ctcagtcaat tcggctgttt cctgtgggtt catacctgct
    11221 gctggttcat ctaagaagag aattttaggt tccgtagcaa gggcacgaac aatttccaaa
    11281 cgacgttgtt gtccgtagga aagattttta gcaagagtct ctgcatcacc atctaaatca
    11341 aagattttca acaattccaa agctttagcc tttaattctt tttcactctt gtaaaaagct
    11401 ggtaagcgta agaaactagt aaaaacatgc tgtttgtgat ggtttccaaa agcaatcaaa
    11461 acattatcta aaactgttaa atctttaaag agacggatat tttggaaagt acgtccaagt
    11521 cccaaagagg caatcttata aggtgatttc ccattcaaaa ggtgaccatc tagggttact
    11581 gttccctcgc ttggttcata aacaccggtc aaaaggttga aaagggtggt tttcccagct
    11641 ccgtttggac cgattaatcc aaccagttcc ccttcgttca attcaagagt cacatctcca
    11701 acagctgtta gaccaccaaa atgtttggtt aactgtttta cttcaagtaa tgccattagt
    11761 tttgttcctc cttcttagat tttttaaaga aacgtgatag gctcagttcc cacgttccaa
    11821 ggagtccacc tggtctgaaa atcattacca ataccaaggc caaagcgtaa ataatcatac
    11881 gcacactagc aacatcttgg agaagcatat ttaaaattcc cagaacaata gccgaaacaa
    11941 tcgcacctgt aatggaaccg agtccaccaa atacaacaat aatcaaaacg ttgattgagt
    12001 tgatgaaggt gtaatctttc ggtacaacag acccgataaa tcctgcctga agtgacccag
    12061 caatacttgc agtaatggca ccaaagacaa aagcgatgat tttaatttta gtcgtattaa
    12121 ccccaactga ctcagcagcg atttcatctt cacgaacaga gagggttgaa cgaccaattg
    12181 ggctacgcaa gaagttcaag gttgcaatgg ttgtaatcac gacaaagaag taaaccattt
    12241 gccaagttgt aaagttagga atccctaaaa tacctgccgc accatttgta aggcttccac
    12301 cattgatgat aaagatacgg ataatttcag aaacacctag agttgctacc gcaagatagt
    12361 cccccttcaa gcgcaaggtt ggaatgccga caagtaaggc aactgctcct gaaagcaaag
    12421 cccctacaag catagctcca aagaaggcac cgtaggttgg tgatttagaa ccaataatag
    12481 ctgctgcata ggcaccaatc gccatgaaac cagcatgacc aagtgaaaat tgtcctgaaa
    12541 aaccaacgat taagttgaga ccaacagcca aaataatatt aattccaatt tgttgtaaaa
    12601 tctgtacata gaatagatta agtactccga ctgaaaccag tacactaatc aagctatagc
    12661 cagctaacaa aaggagtaac catagaatat taacttttaa attttccttc atcgtttaca
    12721 ccttctcttt cacattctta ccaaggatac cagctgggcg gacaatcaag atcaacaaca
    12781 agattccata aacaatggca tcacggaaat ctgacatccc aaaggcagtc gcaaaggttt
    12841 ccaatagacc aatcacaaag ccaccaagag ccgcaccagg aataattccg ataccaccaa
    12901 gtactgcggc aacgaaagat ttaagacctg gagtaacccc catcaaaggc tcaagagagt
    12961 tataataaag agcaatcaga acaccagccg cacccgcaag agcagaaccc aaagcgaagg
    13021 taaagctaat cgtacggttt acattgatcc ccatcaattg cgccgcgtcg ctatctactg
    13081 atactgcacg catggctttc cccatcttag tcttttggac aatgacttgt aacaaaatca
    13141 tcaaaatcaa ggaaatgccc aaaatcatta actgcacatt tgttaagcta attggtccca
    13201 aatcatatcg aactgtttga atcgcttgag ggaaggcacg ggtattggca ccaaccagat
    13261 agaccattcc atactccaat aggaaagaaa ccccaatagc cgtaatcaaa acagcaatac
    13321 gagtagagtg gcgcaaaggt cggtaagcaa gaaactcaat cacgacacca agaatagctg
    13381 tcgctagcat agctacaata agcgctacaa agaaattcat ttggaaagaa ttgatcaaga
    13441 aataaccgat aaaggctccc atcatataaa tatcaccatg ggcgaagttg atgagcttga
    13501 taattccgta aaccatggta tatcctaggg ctaacagcgc gtaaacacta cctagaatca
    13561 aaccatttac gagttgttgg agcataagat tcactctttc tatttataat tccgaggggt
    13621 ttccctcact ttttgatagg ttcttatact caatgaaaat caaagagcaa actaggaaac
    13681 tagccgcagg ttgctcaaag cactgctttg aggttgtaga taagactgac gaagtcagtc
    13741 acatatataa tccaaggcga cgttgacgca gtttgaagag attttcgaag agtattaaat
    13801 atcgaaacag ggagtgagtc aaaggctcat tccctatttc aacatttttc tattatggtt
    13861 ttacaacttc tgctacttca actttaccat tgttcatggt catcatgtaa gcagttttga
    13921 ctgtgttgtg gtctgcatcg aagcttgttt gaccagttac accttcaaaa tcttttgttt
    13981 tagcaaggtt atccttgatt tcacctgaat ttttagcacc ttttgctgcg tttgctacaa
    14041 ggtgaactga atcataagcc aaggctgcaa atgttgaagg ctcttcattg tacttagcac
    14101 ggtaagcgtc aaggaaggct ttagctttag ctgaaacttc tacagtagtt gagaagcctg
    14161 agataaagta gatgtttgat gctttttcag cagttgcttg ttgtacaaac tcctcaccgt
    14221 tgaatccatc accaccaacg attggtttgt caattcccat gccacgcgct tggtttacaa
    14281 tcttaccagc ctcattatag taaccaggaa caacgatagc atcaaagtct ttccctttca
    14341 tttttgtaag ggctgcttgg aagtctgtgt cacctgctac gaaagtttca tctgcaacga
    14401 tttcaccctt gtatgactcg cggaaagatt ttgcaatccc tttagcatag tcactggcat
    14461 tgtcagtgta aagaacaact ttcttagcat ttaatttttc agaaacatag tttgagataa
    14521 tttttccttg gaagctatct tggaaagttc caataaagag gtaatcttga cctttagtca
    14581 atccatcttg agtcgcactt ggtgagatca atggaacacc tgcttttgta gcgttcgcta
    14641 ccgcagctgc agtcgcacca gatgtcgcag gtcctacgac tgctgatact ttagattggg
    14701 ttacaaggtt agttgtaact gaagcagcct cagctgtttc agacttatta tctttatcga
    14761 ctacttcgat ttgttttcca tcgataccac ctgcggcatt gatttcatca acagccaatt
    14821 gggcaccttt ttgttcagct gttccgtatg cagctaaaga acctgattct tcaaagttaa
    14881 acccgatttt aattgtcttt tcctctactg agttaccagc agtgttgact gctccagact
    14941 tcacttctcc acaggctgca agaagtgcta cacttgcaag cgccacaaac gatagggcaa
    15001 attttttctt catctttttg tctccttatt tcttaaatag tctgttaaga atataccgaa
    15061 ttatcagaaa attgtcaatg ttttccttga actttttcga tgataacgtt ttcctatcga
    15121 taaagattgc ccacaaaggg tgtttccaac tcttggatat gacaaaccct aactttttta
    15181 ataaattttt ccttgctcaa gcgtccgacc aattgctcca cttcttgagt tggaacatag
    15241 agttgtaagt aacgatgttt cttggaatga taggtaatat ctccataatc ctgcagtttt
    15301 ttggcatcac gattatagta aagatagata atcaagccag agcgattgac tttttcaaac
    15361 atgataaatc ctctttctaa gaaatctctc cctattatac caaaaaaagc aaacccagtc
    15421 gagtttgcct tctatcatca ttagttcaat gaattgttgg ccataatttc atcaataaag
    15481 ccatattcaa gtgtttcatg ggcgctcatc cagttatcac gttctgcatc tgcatggact
    15541 ttttccattg actgacctga attttcagcc aagatttttt ccaaggtatt acgagttttg
    15601 agcaagtgtt ctgcagcgat agccatatca gtttgttggg taccaccacc tgtaccgccc
    15661 attggttggt gaatcatgta ttcagcattt ggaagcatga aacgtttgcc ttttgctcca
    15721 cttgatgcga tgacagtccc catagatgca gccattccca taacaatggt ttggacatct
    15781 gccttgataa agttcatggt atctacgatt gccaaaccag ctgaaacaga accacctggt
    15841 gtattgacat aaaggtaaat atcttttgta ctatcttggg catccaagaa aagcaattgg
    15901 gcaataacag agttagccat attgtcttca accggacctg tcagcataat gatgcggtct
    15961 ttgagaagac gtgagtaaat atcgtaagaa cgttctccac ggcttgtttg ttcaataact
    16021 acaggaatca ttcatttctc cttttgagtt ttaattttgt tggtcaaatg actgaagata
    16081 agactattat aatatcttgg tcaaaaaagg tcaaattttt gctctgcttt cattagacag
    16141 aaacaaaaac ccaacctcct ttcgtgactg gaaatacttt tccaagtcat tcttcttttc
    16201 gatcttattt tgtaccgaac aagcggtctc cagcatctcc aagacctgga acgatataac
    16261 cgtgttcgtt caaacgttca tccaaggctg ctgtaaagat ttctacatct ggatgagctt
    16321 cttgaagggc ttttacaccc tctggagcag atacaaggca gacaaatttg atatttgatg
    16381 cgccacgttt tttaagagaa tcaacagcca agattgctga gccacctgtt gccaacattg
    16441 ggtctactac aaaaatttga cgttggtcaa tgtcctcagg caatttcacc aagtattcaa
    16501 ctggttgaag tgtttcttca tcacggtaca taccgatgtg gccaacttta gcagctggaa
    16561 ccaagctcaa gagaccatca accatcccga tacctgcacg caagattggg acgatggcca
    16621 atttcttacc tgccaattgt ttttgaactg tttttgtaat tggtgtttcg atttccacat
    16681 cttctagtgg aagatcacga agtacttcat accccatcaa cattgcaatc tcatctacta
    16741 gctcacgaaa agcttttgta gaagtatcta tacgacgcaa gattgacaat ttgtgttgaa
    16801 tcagtgggtg attaataact tcaatttttc ccatttttgg aattccttct ttcaatttat
    16861 tcttctcatt ataccaaaaa acggtttaaa aatctttcta aaccatttat ttttgataat
    16921 ttttacatta gatcagcctc tttaagagct gtctgtactg tctcaagtgg taaatgggtc
    16981 aattctgtcc ctttttcttg ataaaggtat tgggcgtagt cgtccattcg gtactggttg
    17041 atataaacca cgcgcttgca gccgacctga agcaattgtt ttgtacagtt gagacaagga
    17101 aaatgggtta cataggctgt aaagcctttg ggaacaccac gctcagcacc ttgaaggata
    17161 gcattgacct cagcgtgaag ggtgcgaaca cagtggcctt caatgaccaa acattcgtga
    17221 tcaatacaat gctcagtccc tgacaccgaa ccattgtaac cagtggaaat aaccttatta
    17281 tcttttacca gaatcgcgcc cactttagca cgtttacaag tggaacgatt cgcaattagt
    17341 agggcttggg ctgcaaaata ctcatcccag gccagtcttt tttcagtcat ctcttttctc
    17401 ctttttctct attttttaaa aaatggtaaa cctaaatctg caatcttttc agctggtacc
    17461 ttcatgccat ccttgatcca ttttagaagg acagagacga tggctgagct ccagaaggaa
    17521 tgaagataag agctgacacc ttttgatttc ccatggtatt tttctagaaa ttcctgcatg
    17581 gcttggacaa agattttttc cagatggtaa tccaaggcca attgaattac tctagcttcc
    17641 tttctggcct cccggaaaag gtgaacccaa accaaataaa ggtctgtctt taaatcgtaa
    17701 tgatgcagct gttccataat attgtggaca gttcgtttaa agacgctctc taaaatttcc
    17761 tctttggagt cataattgcg ataaaaggcc gcacgcgaaa cacctgcacg tttgaccaat
    17821 tcagaaatac taatcttggt cagctccttt ttttccaaga gttgcaagag ggctgtttca
    17881 atggcttctc tggttaataa attggattct tggtttgatt ttctgagatt ttcaagagac
    17941 ttttcagaga ttctacgttc agacataaca ttttctttct acttgtcaca acagacggat
    18001 gatgcttttg tttcaagagt tttcatgata taattgtaaa aaaagacaga acctttgtca
    18061 atgtataact gacaaagatg gagaatttct atgacttgga agattattgc tgactctggt
    18121 tgtgattatc gtcaactgcc aacaccagct attaacacaa cctttgtaag tgtcccctta
    18181 accattcaag tagctgatca ggtctttgtt gatgacgcca gtctcgatat tgaccaaatg
    18241 atggaaacca tgtatgcaac tgcagaagct tcaaaatcag cttgtccaag cccagatgac
    18301 tatttgcgag catttgaagg tgccaaaaac attttcctag taaccatcac aggtaccctt
    18361 tctggcagtc acaatagtgc tcaactagca aagaatattt atctggaaga ccatcctgac
    18421 actaagattc atgtgattga tagtttatct gctggtggtg aagttgacct actcgtagaa
    18481 aaattgaatg acttgattga ccagggcttg tcttttgaag aagtggttga agctatcact
    18541 gcctatcaag aaaaaactaa gttgctcttt gtcctagcca aagtcgataa cttggtgaag
    18601 aacggccgtt tgagcaagct tatcggtacg gtcgttggcc ttctcaacat tcgtatggtc
    18661 ggaaaagcta gtgaaactgg aactctcgaa ttgctacaaa aagcaagggg atcaaagaaa
    18721 tcagttcaag ctgcctatga tgagttagta aaagctggat atgctggtgg ccgtattgtc
    18781 atggcccaac gcaataacga aaaatgttgt caacagctct cagagcgaat ccgtgaaacc
    18841 ttcccacaag cggatattaa aattctacca acctctggtc tctgcagttt ctatgcagaa
    18901 gagggcggtt tgctgatggg ctatgaaatt gattaattgc attcttgaca agaggtgacc
    18961 atcatctctt gtttttttgt ggtacaatag agctatgaaa cattttgata ctattgtcat
    19021 cggtggggga cctgctggta tgatggctac gatttccagt agcttttatg gacagaaaac
    19081 cctcctcatc gaaaaaaatc ggaaacttgg aaaaaaatta gctgggactg gtgggggacg
    19141 ttgcaatgtg accaacaatg gtagcttaga caacctgcta gctggaattc ctggaaacgg
    19201 acgctttctt tacagtgttt tctcccagtt cgataatcat gacatcatca acttttttac
    19261 agaaaatggt gttaaactta aggtcgaaga ccacggacgc gtctttccag ccagtgacaa
    19321 gtctcggact attatcgaag ctttggaaaa gaaaatcacc gaactaggtg gtcaagttgc
    19381 tactcaaata gaaatcgttt ctgttaaaaa agtagataac cagtttgtcc ttaagtcagc
    19441 ggatcaaacc ttcacttgtg agaaactcat tgtcacaaca ggtggtaagt cttatccttc
    19501 gactggttcg actggttttg gtcacgagat tgctcgccat tttaagcata ccatcaccga
    19561 tcttgaggct gctgaaagtc ctttattaac agattttcca cataaagcct tacaagggat
    19621 ttctctggac gaggtgaccc taagttatgg taagcatgtc atcactcatg atttactctt
    19681 tacccacttt ggtttgtcag gtcctgctgc cctacgcatg tctagctttg tcaaaggtgg
    19741 ggaggttctc tcactcgatg ttttgcctca actttctgag aaggacttgg ttacatttct
    19801 agaagaaaat cgggaaaaat ccttgaaaaa cgctttaaaa accttgttac cagaacgctt
    19861 ggccgaattt tttgtacaag gatatcctga aaaagtcaaa caactgactg aaaaggaacg
    19921 agaacaactt gtccagtcca ttaaagaact taaaattcct gtaactggaa aaatgtccct
    19981 tgcaaagtcc tttgttacca agggtggagt cagtctcaag gaaatcaatc ctaaaaccct
    20041 tgaaagtaag ctggtacctg gcctccactt tgcaggcgaa gttatggata tcaatgccca
    20101 cacgggtggc tttaacatca cttctgccct ctgtaccggc tgggtggcgg gaagtctgca
    20161 ttatgattaa agattaaagg ttgtgaagaa tgaacagaag agaggcagtc gaatttgtta
    20221 acatgtgtat gattaaaaac ggagataagg tcctggtcca agaccgagtt agtcccgact
    20281 ggtctggcat tacttttcct ggtggtcatg ttgaacgtgg cgaatccttt gtcgatgctg
    20341 tcattcgtga agtgaaagaa gaaactggtc tgatcatttc caaaccccaa ctctgtggta
    20401 tcaaaaactg gtatgacgac aaggattatc gttatgtcgt ccttttttac aagacagaac
    20461 actttactgg tgaactccag tcttcagacg aagggaaagt ttggtgggag gactttgaaa
    20521 atctttctca tctaaaactt gcaaccgatg atatgtctga tatgcttcgt gtgtttctag
    20581 aagaagatct cagtgaattc ttttactaca aaaacggtga cgactggctt tatgatttga
    20641 agtaaaaaat aggctaggaa attcctagcc tatttattct gcatttgtct gaacgtaagc
    20701 cgcaatggca tctgacgtgt actgggctgt ttcagaacca aacttgtcaa tgttttcctt
    20761 aaactctggg ttgtagacgt aacctttacc gatatgaccg aatacctcaa tagagcagtc
    20821 aaatccataa gtgcgaatgg cttgcaagag cttggctgct tgctcctggt tttcggttgc
    20881 tgttgcaggt aaaccaactt gaagattttg tgccaaagtt tgaaagactt ggttaaaggc
    20941 ggccgtagcc tcgtcttcgt gacctttttg gcgttcgagc gcttgtccca tgacttcttg
    21001 accatatttc tctaccgctt cttggtggta tttttgattg tcttgatagc taaatcccgt
    21061 gaatttttcc tcaatggtca tttttctttc tcctttttgt tcttgaatag ttttttgcaa
    21121 ggtggaaatc aaggtatcca gatgttgcct ttcgcgagtt agatagtcca actgcctagt
    21181 caaatggggc aataaatctg tcctttcttc ctttaacagc tctgctattt tctctaaaga
    21241 aaagcctaga tatttgtagt aaagaatgac ctgaaggcgt tccaaatcct cttgactgta
    21301 ggttcgatag ccgttttccg actttaaggg gaccaagagt cctatcttgt catagtgatg
    21361 cagggtcttg acagagacac ctgaaagctg cgcagcttct tttatatggt acatgatttc
    21421 ctccttacaa tgatagtata acccctcccc ttaggtcaga gtcaagcgtt tttgagaaaa
    21481 ttttttaact tttttgagaa aggtgtgaaa acttgaaaat gattttcttg acagacctta
    21541 gaaaacatga taggatagtc aagtctatca atcaaacaga aggctatttg acaactcaag
    21601 tagctttttc ttattttgaa aaaggagatc agagtttaac tatgtcagaa aaatcacaat
    21661 gggggtcgaa acttggtttt attctagcat ctgctggctg gccatcgggc ttggttccgt
    21721 ttggaagttt ccctacatga ctgctgctaa tggcggtgga ggctttttac taatctttct
    21781 catttccact attttaatcg gtttccctct cctgctggct gagtttgccc ttggccgtag
    21841 tgctggcgtt tccgctatca aaacctttgg aaaactgggc aagaataaca agtacaactt
    21901 tatcggttgg attggcgcct ttgccctctt tatcctctta tctttttaca gtgttatcgg
    21961 aggatggatt ctagtctatc taggtattga gtttgggaaa ttgttccaac ttggtggaac
    22021 gggtgattat gctcagttat ttacttcaat catttcaaat ccagccattg ccctaggagc
    22081 tcaagcggcc tttatcctat tgaatatctt cattgtatca cgtggggttc aaaaagagat
    22141 tgaaagagct tcgaaagtca tgatgcccct gctctttatc gtctttgttt ttatcatcgg
    22201 tcgctctctc agtttgccaa atgccatgga aggggttctt tacttcctca aaccagactt
    22261 ttcaaaactg actagcgctg gtctcctcta tgctctggga caatctttct ttgccctctc
    22321 actaggggtt acagtcatgt tgacctatgc ttcttacttg gacaagaaaa ccaatctagt
    22381 ccagtcagga atctccatcg tagccatgaa tatctcgata tccatcatgg caggtctagc
    22441 cattttccaa gctcgatccc ccttcaatat ccagtctgaa gggggaccca gcctgctctt
    22501 tatcgtcttg cctcaactct ttgacaagat gccttttgga accattttct acgtcctctt
    22561 cctctttgcg actgtcactt cttctgtcgt gatgctagaa atcaatgtag acaatatcac
    22621 caatcaggac aacagcaagc gtgctaaatg gagtgttatt ttagaaattt tgacctttgt
    22681 ctttggcatt ccttcagccc tatcttacgg tgtcatggcg gatgttcaca tttttggtaa
    22741 gaccttcttt gacgctatgg acttcttggt ttccaatctc ctcatgccat ttggagctct
    22801 cttcctttca ctttttacag gctatatctt taaaaaggct cttgcaatgg aggaactcca
    22861 tctcgatgaa agagcatgga aacaaggact gttccaagtc tggctcttcc ttcttcgttt
    22921 cttcgttccg tcattccaat catcatcgtg gtcttcatcg cccaatttat gtaatcaaaa
    22981 ggacttgagt aatgaactca ggccctttct tttttatgga tgactaacaa ttaattctaa
    23041 atcttgccct tccagagtcc aagcttcaac atcacttggt aggataaagt gtctcccttt
    23101 ttggattgga tagtttttcc cgtcaacagt tagttgacct tgaccagcca agacactcaa
    23161 caagctgtag tcagctgtct tttcaaagtt aacttttcca gtaatttccc atttgtaaac
    23221 tgcgaagaaa tcattggata caaggagagt ggaacgtaga tcatctgctt tgattgtcac
    23281 tggacggcta tttgcgggtt caccaatgtt caagacatcg atagattttt caaggtgaag
    23341 ttcacgtaaa ttgcccttgt catccttacg gtcaaagtca tagacacggt aagtggtatc
    23401 gctagactgc tgggtttcaa gaatcaagat acccgccccg atagcgtgca tggtaccgct
    23461 tggtacatag aagaaatctc ctgctttaac tggcactttt gtcaacagcg catcccagtt
    23521 cttatcctcg atttgctggc ggagttcttc ttttgacttg gcattgtgac cgtagataat
    23581 ctctgaacct tcatctgctg caatgatgta ccaacattct gtttttccaa gttcgccttc
    23641 atgctcgagt ccataagcat cgtctgggtg aacttggaca ctgagccagt cgttggcatc
    23701 gaggatcttg gtcaaaagtg gaaatacagg ttccggacga ttgccaaata attcacggtg
    23761 ttccgcatac aaagtagcaa gatctgttcc ctcgtaacga ccattggcaa ctttagaaac
    23821 accatttaga tgagctgaga tagcccagta ctctccgact ttttcacttg ggatgtcgta
    23881 gccaaactca tcacgtagct tggtaccacc ccagattttt tcttgcataa ctgattgtaa
    23941 aaataatggt tctgacatct taatctcctg tctgattttt ctcccctcat tatagcaaaa
    24001 aaagagttcc atttgaactc ttttttacat cttataaagc agggagaaga ttttataaaa
    24061 atagtaaaca aatgtgctct actcgatgct tacaccattg ctataaatga catgtaccaa
    24121 tagaaggact tcttcttgct acgtttgaga gtcccatttc ctacattatc tcgatctaca
    24181 tagataaagc catagcgctt attcatttcc cctgtgccag ctgaaaccgg atcgatacag
    24241 ctccaagtcg tataaccaag caagtcaatc ccatcttggt aaatggcatc tcgcatggcc
    24301 ttgatgtggg cctctaagta agtaatccga tagtcatctg ctacataacc attctcatcc
    24361 ggtgtatcca tagcaccgag tccattttct acgataatac taaactaaaa tcaaaaatca
    24421 ttatacaata gtaatatgaa atcaattaaa gaagacatcc aaaccatcaa aacactttta
    24481 aaagactctc gtacagctaa atatcataaa cgccttcaaa tcgttctatt tcgtctgatg
    24541 ggcaaatctt ataaagagat tatagagctt ttagactgta atcaaacaac gatttggcga
    24601 aatgtaaaaa aatatgagga gttcggactc gactctctcc ttcaagaaac acgtggtggt
    24661 cgtaaccatg catatatgac agttgaggaa gagaaagcct ttcttgcccg ccatttgaag
    24721 gctacagagg caggagaatt tgttacaatt gatgccttat ttcaggctta taaaaaggag
    24781 ttaggtcatt cctacacacg tgatgccttc tatcaactgt tgaagcgcca tggttggcga
    24841 aatattacgc cacgtccaga acatcctaag aaagcagacg ctcaaaccat tgttgcgtct
    24901 aaaaataaaa tctcaattca agaagacaag aaagcgtttt aaatatagta gacgttttca
    24961 taaggttcgc ttgatgtacc aagatgaggc tggtttcggt agaatcagta aactgggatc
    25021 ttgttggtct ccaataggag taggtccaca tgtccatagt cactatatac gagaatttcg
    25081 ctattattat ggagctgttg atgcctatac aggcgaatca tttttcttaa gagctggtgg
    25141 atgtaatact gagtggatga acgtcttttt agaagagctt tcacaagctt atccagatga
    25201 ttatttttta ctcgttatgg acaatgctat atggcataaa tcaagtacct taaagattcc
    25261 gactaatatt ggctttgcat ttattcctcc atacacacca gagatgaacc cattgaacaa
    25321 gtgttgaaag agattcgtaa acgtggattt aagaataaag cctttcgaac tttggaagat
    25381 gtcatgaatc aacttcaaga tgtcatacaa ggactggaga aggaggtgat aaagtccatc
    25441 gttaatcgga gatggactag aatgcttttt gaaagcagat gagtattata tgcaatttct
    25501 ttatataaaa agaccggatt gctccgatct ttcaatagtt catattctca atttctattt
    25561 taaaaatagc taaggttaac gtcaaatgac tacgcgacct atttcatacg ataaaaatca
    25621 agcactagac cagcaggtcc ttgaactaat aaggactctg ttccccaatc ggttacagtt
    25681 ggtccgtgta aaacctttat accaagctcg ttcaaccgtt tgtagttctg gtctacatcc
    25741 tcaacctcga tatgaataat gattcctgac tgaaagtttt ccaaaggaac caaatgattt
    25801 tgtgacaaca taaggcagtg actaccaatc gtaaactgag caaaaccatc atcagcataa
    25861 tctgcctttt tatccaagat atgctccaag tcagcacaga cttggggaac atttgaaacg
    25921 ataatatcta attgatttaa attcatttac tctcctccat aaaaagaccg gattgctccg
    25981 atcttttaaa gttctgctct atgaaaatca aagaataaag tctacaagtt tcatatttga
    26041 ttttcggcga gaggaattat ttaattgcgc gtgattgcaa tccttcttct tccaagaaga
    26101 gacggaatgg tacgagttct tctgcttcgt atttttcctt gaaggctttg atagcttctt
    26161 ctgagtgaag ttttggatcc aattcaagta cttctactgg aagtggacgg tgttgagtga
    26221 tgcgagcatc gatgacaaca gttttacctt ctttgttcaa tttaacagct tctgcaacaa
    26281 ctgcatcgat gtcttcgata cggtcaactg tgaatccaac agctccttga gcttccgcaa
    26341 ttttagcgta gtcagcgttt gtgaagtcta caccaaacaa gtgtttgttt gtatcttcgt
    26401 atttgttctt gatgaagccg tactcagcat ttgagaagac aaggttgata actggaaggt
    26461 cgtattgaac gtttgtgata acgtctgggt agcacatgtt gaatgctccg tcacccatga
    26521 tgttccatac ttggcgatct ggattgtctt tcttagcagc gataccacca ggaagggcaa
    26581 tacccattgt cgcaaagagt ggagatgtac gccacatgtt cttaggtgtc atgtgaaggt
    26641 gacgagtaga tgtttgagta gtgttaccta cgtcgattga gtagatagcg tcttgatcag
    26701 catgtttgtt gattgcattg taaacttgat acaattgcaa ttcaccctca gttttacctt
    26761 cgagtttgtt catgtaatca cgccagtttt gattgttctt aacgtttgca cgccaccatg
    26821 gagttgattc aactgggttt actttgtcaa ggatagcttt agctgcttga ccagcatcac
    26881 caaggattga agcgtcaagg gcatgacgtt taccaagttt gtaagggtcg atatcgactt
    26941 ggatgaattt ttcagtgttc ttgaatgctt cgtaaacttc agcgaatggg aagtttgaac
    27001 caaggaaaag aactgtgtct gcttcaaaga ccacttcgtt ggctggtttc caaccaacac
    27061 ggtaagcaga acctgtcaaa ccttcatagt tccattcgaa agcttcaaag tttttaccag
    27121 ttgtgatgat tggtgctttg attttacgtg acaattcagt aatcacttca ccagctttaa
    27181 caccaccaaa tccagcatag ataactgggc gttcagcatt gttcaagatt tcaacagctt
    27241 tgtcgatttc aacttcgttc aaagcaggag cgatgaatga gcgttcgtat gaacctgaac
    27301 cgtagtatga gttttcatcg atttcttgga aaccgaagtt tactggaatt tcaacaacag
    27361 ctggaccttt tttagaaact gcagcacggc aggcttcgtc aattactttt ggcaattgct
    27421 cagcgtaagc tacacgtttg ttgtaaacag cgataccgtt gtacattggg ttttggttaa
    27481 gctcttggaa agcatccatg ttcaattcgt taactggacg tgatccaagg atcgctagga
    27541 atggagtgtt atccatagct gcatcgtaaa caccgttaat caagtgagtc gcacctggac
    27601 cacctgaacc aactgcaacc ccgattgagc cgccgaattt agcttgcata accgctgcaa
    27661 gagcacctgt ctcttcgtgg cgaacttgta agaaacggat atctttgtct tcagccaaag
    27721 catccatcaa tgagctgagt gttcctgatg ggataccgta gattgtatct acgccccatg
    27781 ttttcaatac gttaagcatt gctgcagatg cagtaatttt cccttgagtc ataatgataa
    27841 ctctccttca atttttttaa acttggagaa tacgattaca tagaattgga aacgttctcc
    27901 aaatttttac tattccactg tatcatattt atgctgactt ttctaaaaat ctgctcaaaa
    27961 ctctctattc tctattctaa tacagttttg aaagtgttgt catttctgtt ttataacaaa
    28021 gaaatctagt cattactttt agtctatttt actaaaattt aacagaaggg aactggtcag
    28081 aacagataca gaactaaagg ccatggctag acctgccaat tctgggttga gagccagtcc
    28141 aacacctgaa aagactcctg ctgcaatcgg aattccgaca acattgtaga taaaagccca
    28201 gaaaagattg agtagaattc gatgaaaggt tttcttactc atatcaaagg cacgaaccac
    28261 tcctaaaaga ttattggttg tcaacaccaa atctgctgac tcgatggcga tatctgttcc
    28321 agctcccata gcaatcccca catctgctac actaagggca ggagcgtcat tgataccgtc
    28381 cccaacaaag gctactttcc ctgactgttg cagtttatgg atttcatggg ctttttcttc
    28441 tggcaagacg cctgcaatga cctcttcaat tccgatttga tctgcaatag cacgcgccac
    28501 accagcattg tctcctgtca gcatgactgt tcggagacca cgttttttta gctgactgat
    28561 gactagctta gcattttcct taggaatatc ttgcaaagca agcaagcctt tgatttcatt
    28621 gtcaacagct aagaacacaa ctgtcttagc ttctttttct agttcttcta gtttatcttg
    28681 ataagtatta gaaatatcca tgccatccag cattttagca tttccaagta aaacttgttt
    28741 tccattgatt cgccctgaaa cacctttccc gtgcaaggcc tgaaaatttt caacagtttg
    28801 aaactcaagt ccagcttcac tcgctcgctt aacgatagcc tcagccagtg ggtgttgaga
    28861 agcatcttcc aaggaggctg ccaatccaaa cacttctact tcgtcgccga tgatatctgt
    28921 taccacaggt ttcccttccg tcaaagtccc ggtcttatca aagacaagag tttgaacttt
    28981 ctggatttcc tgtaagacag ttccattttt gaggagaacc cccatcttgg cactacgtcc
    29041 tgtccccacc ataagggctg tcggtgttgc aagtcccaag gcacaaggac aggcgataat
    29101 caaaaccgcc actccgtaga gaagagagga cacaaagcta gctccaagca cgaccacact
    29161 atccctgagc aagacgaacc aaacccaaaa ggtcatgatt cctaaaatga caactactgg
    29221 gacaaaaatc cctgaaatct tatccgtcaa gtcctgaatc ggcgcacgac ttgtctgagc
    29281 tttcttcaca aaatccacaa tctgagccaa aacagtctct gagccaactt tttctgctct
    29341 aaagacaagc gttccactat gattgatggt tgagccaatg acagtatctc caactgtctt
    29401 gtccacaggc agactctcac ctgtcaccat ggattcgtca atactagaga caccttctac
    29461 tacgacacca tcaacagcaa tcttttcacc gggacgcact cgaatcaggt cgcctacctt
    29521 gacttgttcc aaaggaactt ggacataact atcatcactc aagacttctg cggttttagc
    29581 ttgcaagtcc agtaatttct ccacagcttg ggacgtattt tttctcattt tttcctcaaa
    29641 aactgctccc aaaagaacga aaaagaggat aaatccagca ctttcgaagt aaacagggag
    29701 accagcaaag agagcaacta ggctatagaa ataagccact agagttccca gcgcaaccaa
    29761 ggtatccatg ttggcattgt gctttttaaa actggcccaa gcactctgga tatatggctt
    29821 acctgcaact aacataatag gcgttgttgc tagaaaggtt ccccaatgca tgacttgatg
    29881 actaatgcta cctgtcaaca tcccaatcat gagaatcaca agaggcacag taaagatact
    29941 agtaatccaa aaacgttgca ggagagatag agattttcga gtcttctcaa cgactatata
    30001 gcttcccttt tgcatcttca tgccacaaga aaattcatgt cgccctaatt cttgaggtgt
    30061 aaaacgaatg actttctcct catctacgcc gattggttcc aagatacctt cttcttcaaa
    30121 cagaatttcc ttataacagt ttgaaggagt agcacgatga aaggtaatct cagctggaat
    30181 tcccttttga agctggatat gggctggatg atagcctttt tcagctcgga tacggatttt
    30241 ttgaatgcca ttttctaagc ttgctttcac aatttctgtc atagtctcca cctactctac
    30301 aatcatcttg ccgtgcatca tgttcatacc acaagcaaag ccaaactctc caacctgctc
    30361 aggcgtgatt tccactacat actcttcccc cattggcagg ttcgcatgta caccaaaatc
    30421 tggaaaaaca atttgatcca gacatggtga aggatccttg cggtcaaaga caatgcgtgc
    30481 tggcactgat ttcttgagga caatcaactc aggagtatag cctcccatga cttccactcg
    30541 aatctcttgg tatccgtttt tttgctgggc tttttgtcca gatttttcag gctttttgaa
    30601 aaaccaaaac aagataaacg cgataagggc aatacaaata atggttacaa tactatttaa
    30661 catggcgtct cctttacata caattacatc ttacttctgt tacagcactt gatttcttct
    30721 ctgaaatcac agcttccaag tcttccaagt cagtctgagt aaattcacat tctacaatca
    30781 agtcagccaa caaattccta atcctacggg aacaaacctt gtctttgata tcttggacaa
    30841 gtaaatcccg actttggtct agagttaaaa gcgctgaata aacaaaggac ttgccttctt
    30901 ttttccgagt cagacactct ttctcaacca aacgagccaa aagagtttga atggtcgact
    30961 tggaccagtc gaaccgctct gccaaaaccc taatcaaatc tgtactggtc tgctccccct
    31021 gcatccaaat aatcttcatg acctgccatt ctgcatctga aatctgcatt accatacctc
    31081 caaaatctac atttgtcaat tacactcatc agtatactct taaaatctac atttgtcaat
    31141 tatagaaata atattttctt cgaaaaatag aattttaatc atttgaaaaa cgatttgcag
    31201 tcaaatatta ctatataaac aataaaaata tgctatacta aagaaaaaag aaaacaacca
    31261 ctaggggtgc gtaaagctga gattaacgac tgttagatcc ctctgactca atctaggtaa
    31321 tgctagctga tggaagtgga aatgataatg gggactagca gtcttctatt gcctttctaa
    31381 aacagactag cttgttctta agaatacaaa cttcagttgg ttgggaggtt ttagatgact
    31441 tatttacccg ttgctttgac cattgcaggg actgacccta gtggtggtgc tggcattatg
    31501 gcagatttaa agtcattcca agcgagagat gtctatggaa tggctgttgt aaccagtctt
    31561 gtcgctcaaa ataccagagg tgttcagcta atcgagcacg tttctcctca aatgttgaaa
    31621 gcccaattgg agagtgtctt ttctgatatt ccacctcagg ctgtaaaaac tggaatgttg
    31681 gctactactg aaatcatgga aatcatccaa ccctatctta aaaaactgga ttgtccctat
    31741 gtccttgatc ctgttatggt tgctacaagt ggagatgcct tgattgactc aagtgctaga
    31801 gactatctca aaacaaactt actacctcta gcaactatta ttacgccaaa tcttcctgaa
    31861 gcagaagaga ttgttggttt ttcaatccat gaccccgaag gcatgcagcg tgctggtcgc
    31921 ctgattttaa aagaatttgg tcctcagtct gtggttatca aaggcggaca tctcaaaggt
    31981 ggtgctaaag atttcctctt taccaagaat gaacaatttg tctgggaaag cccacgaatt
    32041 caaacctgtc acacccatgg tactggatgt acctttgctg cagtgattac tgctgaacta
    32101 gccaagggca agagtcttta ccaggcagtt gataaggcca aggcctttat cacaaaagct
    32161 attcaagatg cccctcaact cggtcatggt tctggtccag tcaaccatac aacttttaaa
    32221 gattaagaaa aaaactctct agttcccact ttaagggaat tagagagttt ttatactctt
    32281 cgaaaatctc ttcaaactac gtcagcttcc atctgcaacc tcaaaacact gttttgagct
    32341 gacttcgtca gtcttatcta aaacctcaag gcagtacttt gagcaacctg cgactagctt
    32401 tctagtttac tctttgattt tcattgagta ttaattagga aagaatgtca tgcaactttg
    32461 ttaaaaaggc ttgcgttttt gcctcaatat cttctgcttg catcaaatca cgtacaacag
    32521 ctacaccagc tatgccagtg cccataagct gatcaatatt ctccgaagtc aagcctccaa
    32581 tagcaactac tggaatggca accgtttggc aaattgtttt caaggtcgat atcagagtaa
    32641 tgggcgcatt ttccttggtg gtggttggga aaatggctcc tgtacccaag taatctgcac
    32701 ctgatttctc cgcttccaga gctcttttaa ccgttttagc ggtgacaccg aggatttttt
    32761 caggacccaa gactttgcga gctaccgaaa ctggtaattc atcatctccg atatgcagac
    32821 ctgctgcatc aaccgcaaga caaacatcca accgatcatc gattatcaag gggacctgat
    32881 aggcatctgt tatttccttg acttgttttg ccagttgata atattgattg gttgtgagat
    32941 ttttttctcg caattggact atggtaaccc ctgaacggca ggccgtctca acttttgcaa
    33001 gaaagctttc cacggaatct tgatagcgat tggttaccag atatagtcta agtgcttctc
    33061 tattcataaa cctctccttt gatggtatct agccaatttt catctcttct taggagcgaa
    33121 agctgattga gtacttggta acgaaattct tccaatccca ttccttgaac aactattttc
    33181 tcagcagcga tattgagata agagactgct aagcaagaag cttcaaaacc agtctttcct
    33241 tggctgagaa aaacggctgt taaggctcca accaagtctc ctgtccctgt tatccagtct
    33301 aattcagtac agccattctc cagtacagca acttgattct ccgaaacaat aaggtccttg
    33361 gggccagtaa ctaagaaaga cataccagga taggtctgac accagtcttt caagacttga
    33421 agcaaatcct ccgtttcttg atctttagca ctcgcatcga ccccaacgcc gtggtgcttt
    33481 aatccaacaa gacttcgaat ttctgacatg tttcctttaa ggaccgtagg tctatagtct
    33541 aaaaggtctt taactaagct cttacgaatg gatgaagtcg ttacgccaac cgcatctact
    33601 accatcggga gagaagattg gtttgcatac gaagctgcca tgcggattgc tttttccttc
    33661 tcagctgaca aatgccccaa attgatgaag agagcctgac tttgcttagt aaaatcaaga
    33721 acttcacggg gatcatctgc catgacaggt ttgcatccca gagccaaaat cccatttgcc
    33781 agcatctcac aagaaatctc attggtcata cagtgaatga gggaactaga gcctatagga
    33841 aaaggatttg tcaatgcctg catcattcta tcctttcagc aaagaaatat ccttgcactt
    33901 ttttaaagaa ttcctgcttg attaaaaatc taaatgcaat aaaggaaatc gctgtaccaa
    33961 tcaaggttgc tccgaaaaat cgaggagtgt agataaacca actaagctta gcagccgatc
    34021 ctgtaaagag caccataaca ggataggaaa caatagaacc aataatacct gttcccacaa
    34081 tttctcccaa ggcagaaaag taaaattttc gaccgtactt ataaaagaga cctgctagaa
    34141 gggctccaaa agtcgctcct gtgagagcta aaggcggaat cccttgagtc gtcatacgga
    34201 taaaggctgt cactgtagcc atagccaagg cataaacagg tcccatcatg attcccgcta
    34261 gaatattgac tacactggac atcggtgcca ttccctcaat ccgaaagata ggtgtaagga
    34321 ctacatcaag ggcaatcatc atagataaaa tggtcaattt gtgaacttgt agttggtgct
    34381 ttctcatgtt tctattcttc tcctttttct aaagactgta aatcgctctt ccatgtctgg
    34441 tgttggtaag ccatctccca aaacttagct tccatatgaa cactgatgtg gaaggcatct
    34501 agcatttttt gcttatctgt ctcatcactt tctcgataaa gctgattgac cagtgctccc
    34561 tcctctctga tctgttgctc taactcatcc gtaatataag tttcaatcca ttgttgatag
    34621 agaggatttg gtgatggttt aagattaagt gatttgccta tatcatggta taaccaagga
    34681 caaggaagca agcttgcaaa agctatggct aagttcggtt ctgcaaattg cctataaata
    34741 tgagaaatgt aatgataaca ggttggagcg attggatgtt gctccatttc ctggtcgttg
    34801 atttccaatt ccttgaaaaa ttgttggcga ataaataact caccctccac taaaccctga
    34861 gcattttgtt tcaagagtct tttcatctct tggtttgaag tcttatcagc caaaagatga
    34921 tagatttctg agaaagcctt cagatagtag gcatcctgaa tcaggtaata gcggaaaatg
    34981 gcaggttcta aattcccctc ttgtaattgt aaaataaagg gatgatgaaa ggaagcctgc
    35041 caagctttct tggataattc catcgcaata tctgtaaatt ccataataac tcctttataa
    35101 aaatagactg gtttgaagca ataaaaagaa aagcaggtag attaattttg tttttttagg
    35161 aatataaaaa gtccgatagc tattcttcaa ctgtgcatgt tcgtcatatc cgtgagcaga
    35221 tagagctctc aggtaaagat ggcgccacct aaagactgtc atcagaacct tactgtaaat
    35281 caagggcgac caaaaatgta gtttttgacc acgtaatagg caagcttctt tgagggactt
    35341 gatttcttgc tgaatgagag gaaaagaatt gaataccaca atcaaggcat aggaccaaga
    35401 gcgtgatagc cccttttgag ccaagtacaa gagaagctct tttagtgaaa tagaggaaac
    35461 aaagacaagg ccgatacaaa ctgtcacaaa ggccctcgtt ccaagcatga ctgcctgtga
    35521 agcatctccg tgtaactgaa ctgcccagta gttggcaaaa gatggtaaaa tggcaagtat
    35581 gatcatccaa gctaacattt taaatcgacg gtaatagagc ataaagagaa tacaaaatgc
    35641 gactaccgaa agattcagag caatcgaagg aatgaaagat gtttccaagg ataaaatcag
    35701 caagaagaga ctgataatcg gtgtctgggt tgctactttg accatactat ctcacctccc
    35761 cttgggtatt gctactctga gatgtaagtg gtttggtaat ggtcacttct ttcacatgcc
    35821 gaagaccctg actagtcatc tcaatccaat aatcaaccac agaaatcaaa gggtctaaac
    35881 gatgactaat gagcagaaaa cttcttcctt gattcctctc ctccacaatc cacttgcaaa
    35941 aataatggca ggctctatca tccaaacctg caaaaggttc atctagcaag atcacggaag
    36001 ccttactggt caagatggtc aggagctgaa gaattttttg ctgaccacca cttaattgat
    36061 agggactctt atcgactgcc tgctccaaat caaaatatcg taaagcttga aaaatccgct
    36121 gatttctttc agaatcaggt ccatctaatt gaagctcctc tcgcagactg actcggataa
    36181 actgcttctc agcttcctga acaacacccg tcagatcacg gtacaaactc tttttctttt
    36241 tcaggactgt ccccttccat gcaatgcgcc ccttatactt ttgaaattga agaatagacc
    36301 gaaagagggt tgatttcccg acaccattgt cacccaggat acaggaaatc ccttgataga
    36361 atgtgaaatc agcaattgaa aagagggggc gattaccaag ctcaccagtc acacggttca
    36421 tatggaatag ttccgggcta gaagcaactt cctttgaagc aacctgtgtc atctcatagg
    36481 aagggatttg atttatttcc cttagttttc cgtctcttag ctcaaccata tggtcgatat
    36541 aggctttata gtcagataaa tcatggtcgc acaaaataac tgtcttccca tcaagagcca
    36601 actcttttag aatctccaat atctcgattc tgctcttgcg gtcaatggaa gcgaagggct
    36661 catccaagag atatacccta ggattcatgg caaagaggac agccagcgct gctttttgct
    36721 tttccccacc tgataggtga tgaatgggac ggtgtaagat tgcctcgcaa cgacattgct
    36781 ggacaacctc tgctatttta gaatcaattt cctgaaggtg atagccgata ttctccaagg
    36841 tgaaaatcaa ctcctcaaac aagttctcca tagtaaattg atgattggga ttttgcaaga
    36901 gaatgccaac cttctggaca cgtgcgatga tagaaagctg actgacctcg ctcccatcta
    36961 tcaggacttg accactatag ggaagagaac tgacttgggc aatcatttga aagaggctgg
    37021 attttcctga accactgctc ccaactaaca aggtaaaggc ttgcgcatga aaagtaaaat
    37081 caaacggctc agagaagatt ggggactgaa tcgctcgtag ttccagaccc atctatgctt
    37141 ttcctccagt tgcaaactga tgatagagtt tgacaatggc acgaaccaag atggtacaga
    37201 agaaataaac agaaataaaa cgtaccacaa gcaaggaaag gacaaacgga agggaaaagg
    37261 cgtagtaacc taacttaatg tattcataga caaagctaac aagcgtaatc ccaatactat
    37321 tagcagttag agagagccaa ctttcatagc gattcttagt tacgataaaa ccaaattcac
    37381 ttcccaaacc ttgaaccaag ccagacaaaa gagctcctag accaaattgg ctaccataaa
    37441 ggacttcagc aagcgcagct agcacttctc caatcgttgc acttccgact ctcggaacaa
    37501 agatggcagc aatgggcgca gccatacacc agagaccgaa gaggatttca ttggcaaagg
    37561 cctgcaaacc aagaggtgtt aagagtagac tgagaatatt atacacatat cctgaaccaa
    37621 cgaaaacccc accaaaaaag atagacaaga aagcaagcaa gataacatct tttaactgcc
    37681 attttttcaa cataaaaaac tccttttttt aaagaaaagt ggggcactca agaagaccga
    37741 cctaaatact ttgtatagca gactgaattt agaacagtac acaagaacac taaaatattt
    37801 ctagaaatta atttgaattt tctaattgat ttgttcgcat cttatttcaa tctactatat
    37861 catcttcatc cagtttcgta aaagaaaaaa ctctaattac agatacgaat tagagttcag
    37921 cttacaagat tagacagttc ttttcgacat acgaaaaaaa catttcacat ttcccttcgc
    37981 cagtcttaac tgtatcaggt tcaatgggta tcatctcagc ctaaagcacc ccaaatgcct
    38041 ttattattta attatgtgat tattataaca cacattttat actagttcaa gaaattgaac
    38101 tggaaataca gccttgcact cacaaagaca gcagatcttt cttttgcaaa aaacaaatga
    38161 cctgtttgat gaattagcta ttcaagctga atctagacat agctttttaa aaaaggaaaa
    38221 tcctacttac ttagaatcca aggatagata tctattgttc actcatttcc cgaacagttt
    38281 tttctatatt ttttgcatac gatattgccg aaatgattga aacgccgtca acattggtct
    38341 ttataatgtc tttaatatgt ttcgtctgta tcccaccaat tgcaactaaa ggcatttgtg
    38401 gcaatagttt tctcatcaat tcaagacctt cataacctat agcaccacca gcatcatcct
    38461 ttgactgggt atcaaataca ggaccaacac caacataatc tacatattca acttttgatt
    38521 gttgaaattc ttcctcgttt tttatagaaa gaccaattat tttatctggc atcaattttc
    38581 taatttcatc aacaccaata tcatcttgac ctacatgtac gccatcagcg tcaatttcca
    38641 ttgctaaatc tatatcgtca ttaacgataa atggaacatt gtatttttta caaagttctt
    38701 taatttggat agctagctca agtttttcta agccttctaa agcaccctca cctttttctc
    38761 gaaattgaaa taaggttata ccacctttta aggcttcctc aacgactgta tatagatttt
    38821 ttccttggca agtagtcgtt ccacaaataa aatatagttt tagtaattct ttatgaaaca
    38881 tcttacttca ctcttttgaa tttcttgaca tcttcatctg taatttcata taaggcattt
    38941 ataaattcaa ctttaaatgt tccaggaaga tgtccatttg gacgtttttc cgccatttct
    39001 ccagcgatat tgtaaaccaa cattgctgtt tctaatgatt tcaattcttg acctttttca
    39061 agtccgataa agcttgctac tacagctcct aataagcatc ctgtcccaat gactttcggc
    39121 atcatagcac taccattatg aatcgttacc acttctccat taacagcaat ggcatccact
    39181 tcacctgtta ctactattgg aatattgaac ttctcatttg ctgctagagc aatttcgtca
    39241 atattatcta cgcccgcact atctactcct ttagatgcca catctattcc tactaaagag
    39301 gcaatctctc cagcattccc cctaatcgca gctagtttgt aattgttgat taaatcatct
    39361 gctacttttt ttctatattc tcctgctcca caggctacag gatctaaaac tgctgggaca
    39421 ttatatttct ctgcaatttt cagagcagct tggtataatt tccaattttc atctgtcaat
    39481 gttcctatgt ttattaataa accaccagca tactttaata agtcctctaa atctgctgga
    39541 aactcactca tggctggtga ggcgcccagt gctactaatc catttgctgt gaaatttttt
    39601 actacatcat tggtgatgca aatgaccaaa ggtgcttttt cttttaataa ttttaaactt
    39661 gtcatattga aatccttcct tttcacttta tacgatctac taatttcgat ttatctttag
    39721 ttgagaattt ttttcattta cattgaatga tttatactca atgaaaatca aagagcaaac
    39781 taggaggcta accgcaggtt gctcaaaaca ctgctttgag cctgtagata agactgacga
    39841 agtcagctca aaacactgtt ttgaggttgt ggatagaact gacgtggttt gaagagattt
    39901 tcgaagagtc ttacctcatc aaatttgtaa atatcatgag ccttctctag acatcgtaac
    39961 caatatcaaa aaaagctaat tctaaagcga ctgcttgatt ccagcgttgc tgaagttctg
    40021 tcaaatcttc tcgattttta ccgacacgat tgagttcatc aaccagaaat tgaacccact
    40081 ctgcaaagaa aggacctctg tggagattga tccattccga atgaatatag acttcaggta
    40141 gagccaaatc tttagaaccc cagtctaaat agagaccttc tgcaatgacc agcataacca
    40201 aaagatgggc atagtctgat gaagccactg cccaatacat tagatcctga aaggcttttg
    40261 ttacagggtg caaagtcact tctagatagt tattctctgc tacttttaac tctttaaaag
    40321 ccttttggaa ataaccatct tcatctgctt caagaaagcc tagttgcttg gcaaaacgaa
    40381 gcttggattc aagtttatct gcgtgagcta cgcaggcacc cagcatggat aagaaggcat
    40441 caaagaagtg ataatcttga atcagatagt cctttaagac cttattctca attgtccccg
    40501 caaaaagttc cttaacaaaa cgatgattga ttgcagcctg ccaatccttc tgactgcttt
    40561 ttaataattc tccaacagtc aaacctggct gaaatgcata gtcttgtgtt tccatattta
    40621 cttctcctct ctttacttgt tagtaattaa taaaacagca agaaatatca agcaaaatcg
    40681 taattccact tgatcctttt aaagcacatc gagagcattt acagagagct aactaaacaa
    40741 gcctatccag tttatataaa caaaaaactc caattacaat caagaattag agttgactta
    40801 caagattaga ccgttcattt caccatacga aaaaactgtt cacatttccc ttcgccagtc
    40861 ttaactgtat caggttcaat gggtattatc tcagcctaaa gcaccccaaa tgtctctatt
    40921 atttaattac tgaaccagta tagcaaaaaa tgaaagccct agcaagatat ttgaccgaaa
    40981 aatatcttta tatacaatat attgaaacta gaatagtaca cctctacttc taaaacattg
    41041 ttagaaatcg atttaactgt cctgattgat ttgtcctatt cttatttcat tttactatag
    41101 ttttcgatag cgatttattc ttccaataca cgaagaaaaa cctccacatt cagtgaaggc
    41161 aatctgtttt atcaatacaa ttttaagtca cgagggtcaa ctgggaaggt tgggttgtat
    41221 ggattgtgac ggagcttaaa gtgtttgaca tcttcaatgg tctgagctcc agacaattgc
    41281 ataactgtct tcaattccgc attcaagtgt tcaaagactt gacgcacacc gacactacca
    41341 ccgagagcca agccataaat aacagggcga ccaatagcta ccaagtctgc tcctgaagcc
    41401 aaggctttaa agacgtgttg accacgacga acaccagagt caaagacaat cggcacacgt
    41461 ctatcaactg cttctgccac ttcttgaagc gagtcaaagg cagctggtcc accgtcgatt
    41521 tgacgaccac cgtggttggt tacccagata ccagaagctc ctgcagcaag tgaacgttca
    41581 acgtcctcac ggcattgtgg tcccttgaca tacacaggaa gaccagagta ttcagcgata
    41641 aattctacat cgcgtggaga caagcgttgt ttagctgatt tgtaaacaaa gtccattgat
    41701 ttaccagcac cttctggcag gtattcttca acaatcggca tgccaactgg gaagacaaaa
    41761 ccattacgct tatccacttc acgattcccc cctacagtag catctgccgt caagacaatc
    41821 gctttataac cttcagcctt cacacggtcc atgatgtggc ggttgatacc gtcatcctta
    41881 ctaaagtaaa attggaacca atggggtgtc ccttgaaggg cttcagaaat ctctggaagg
    41941 tcaacagtag agtaagagct ggttgtataa agagaaccaa actcatgcac accacgcgca
    42001 gtcgccactt ccccctgttc atttgccaat ttatgagccg caacaggcgc cataatgatt
    42061 ggtgaagata gtttttcacc tgcaaattca atctctgtac ttggattttc aacatcgcaa
    42121 agtgtatgag gaacgatgag tttgtggtta aaggcgcgga tattctctct taaagtgaaa
    42181 gtatcttccg ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc
    42241 attggctcca aatcataggt attgatgaag tctacatgac cttctgcatt gcttgtttta
    42301 tatgacataa aatgccctcc ttgattaagt aagcgtttgc tttttactac acaactataa
    42361 taactctttt ttcaatcatt ttcaagagtt ttgcttaaaa accatcttat tttttcatac
    42421 aatatactag ccctactttc cttaaactct aaaccttagt ctatattaag tgaatcgttg
    42481 agattttttg ctgccaggca tttgaactac ttttttattt gcggattcta tctactaata
    42541 ctataaaaaa aacaagtgaa taagacacca gaacatcctc taataggacc tatggactta
    42601 tatcagtccc atatatactg tttaagcaag atactatcct agttgacgct aacttcctat
    42661 cccacttatt aattttttat ttatagtgaa tctgaacaaa tcaattgggg aatccaagtc
    42721 aatttctagt actttttgaa cagtttcagc gtactatcgt aaaatgaaat aaaacatgca
    42781 caaattgatt agggaattta aaccaatttc taacaatgtc ttagaaatca aagtgtacta
    42841 tttcaacttc aatatactac acttcatttt ctccttaaat tcagtctctt tttgaatcaa
    42901 aaaatcctct ggtaagagcc agagggtaag aatttatttc attgttggga agagcaatac
    42961 atcacggata gttgttgtat cagtgaggag catgcagaga cggtcgatac cgattcccaa
    43021 accacctgtt ggtggcatac cgtattcaag agcctcgatg tagtcatagt cgatgcctgt
    43081 cgcttcatca tcaccaagtt ctttagcttt agcttgggct tcaaaacggc taagttggtc
    43141 gattgggtcg ttgagctcag taaaggcatt accgtactcc ttagtcatga taaagagctc
    43201 gaaacggtca gtaaagcgtt ggtcttcagg atttttctta gcgagtggag atacagctac
    43261 tggatgtcca tagacaaagg ttggttggat taaagtttct tcaacaaact cttcaaagaa
    43321 ggcattgatg atgtgaccaa cctcagtgta gtgtttctca actggaactt tcttctcagc
    43381 agcgatagct ttagcttctt tcaaagtcat gtcttgccag aaatcgacac cagtaatttc
    43441 tctgatagca tccaccatat gaacacgctt aaatggttcg ttaatcttga tttcagtacc
    43501 ttggtagttg actgggccat cgcccttaac tgatttagca gcatgttgga taatgccttc
    43561 cgtcaagtcc atgatatctt ggaagtctgc ataagcttgg taaacttcga tagaagtgaa
    43621 ctcagggtta tgagtagcat ccattccttc gttacggaag atacggccaa tttcatagac
    43681 acgttccata ccacccacga taaggcgttt taagtgaagc tcagtcgcga tacgaagcac
    43741 catgtcaatg ttttgggcat tgtggtgggt gataaatgga cgggcagcag caccaccggc
    43801 ttcattatga agaacaggtg tttccacttc aaggaatcct ttttggtcaa ggtaacgacg
    43861 gatttcagag atgatttttg aacgagtgac aaagcgttca aagctttcac gattagaaat
    43921 caagtcaagg taacgtttac ggtaaattgt ttcaacgtct gtcaaaccat ggaatttctc
    43981 aggaagagga cgaagagcct tagacaagtg tgtgatgtgg gttgccttga tagagagttc
    44041 tcccatatcc gtacgcatca cttcaccttc gacaccaagg aagtcaccaa ggtctgcttt
    44101 tttgaagatt tcgtagtttt cttcaccgac agcatcctta cgaacgtaga tctgaatctg
    44161 gccttcgcgg tcttgaaggt gggcaaaacc aacttttcct ttaccacgtt tggttaccaa
    44221 gcgtcctgcg atagtagctg tttcgttttt atcgtgtaat tgttctttat cgaggttggc
    44281 atatttatct tttaattctt gtgaatttgc agtacgttca aaacgttttc cgaaaggatc
    44341 gattccttgt tcgcggagcg cagccatttt ttcacggcga acgatctgct ggtcatttag
    44401 ttcttccata tgttctgtag acatgggatc ctcctagttt tactcaaaat tgaatacgat
    44461 gagtcatttt ttgcgatact cccattttat cataaataag caagaaattc acggataatc
    44521 tttaaaaact aaaaagaacc tgacgctaaa atcactggaa agaagtgaaa gtatcttccg
    44581 ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc attggctcca
    44641 aatcataggt attgatgaaa tctacatgac cttctgcatt gcttgttttg tatgacataa
    44701 aatgtcctcc ttaataagta agcgtttact ttgtatatta taaaaatata ttaactcttt
    44761 tttcaaaact tttcaaatat tttgtttgga aatttcagaa attttatgtc tatgataaaa
    44821 atccttataa cggcaataaa aaatagatat tatccaaaga agattttaag tgctacaata
    44881 actgtattat ttctagatgg gaggttctat ttttggattg atccattgtt gaacaatatc
    44941 taccactata tcaaaaggca ttctttctga ccttgcatat tgcagtttgg ggaattttgg
    45001 gatcctttct gctcggttta atcgttagta tcatccgaca ttatcgaatc cctgttttag
    45061 cgcaagtagc gacagcctac attgaattgt cacgtaatac gccccttttg attcaactct
    45121 tctttctcta cttcggtctt cccctaatcg ggattgtcct atcttcagaa gtctgtgcaa
    45181 cgcttggact tgtcttttta ggaggctcct atatggcaga atctttccga agtgggctgg
    45241 aagccatcag tcaaacccag caggagattg gcctcgctat tggtctgaca cctctacagg
    45301 tctttcgcta tgtggttctt ccgcaagcaa cagcggtggt actcccctcc tttagtgcca
    45361 atgtcatttt ccttatcaag aaaacctctg ttttctcagc agtggctttg gccgacctca
    45421 tgtacgtcgc caaggatttg attggtctct actatgagac agacattgcg ctagctatgt
    45481 tggtagttgc ttatctgatc atgctgctac ccatctcact ggtctttagc tggatagaaa
    45541 ggaggacctg ccatgcagga ttcgggaatc caagtactct ttcaaggaaa taatctcctg
    45601 agaatcttac aggggttggg cgttacgatt gggatatcca tcctgtctgt cctcttatcc
    45661 atgatgttcg gaacagtcat gggaatcatc atgacctccc attctagaat catacgattt
    45721 ttaacacgat tgtatctgga atttatccgt atcatgcccc agctggtgct actcttcatc
    45781 gtttactttg gcttggctcg aaactttaat atcaatatct caggtgagac ttcagctatt
    45841 atcattttta ccctctgggg aacagctgaa atgggagact tggtacgtgg agctatcact
    45901 tctctcccta aacatcagtt tgaaagtgga taggcactcg gcttgactaa tgttcaactt
    45961 tactaccaca tcatcatccc acaagtctta agaagactgc taccgcaggc tatcaatctt
    46021 gtcactcgga tgattaaaac cacttcatta gttgttttga ttggggttgt ggaagtgacc
    46081 aaagttggac aacaaatcat cgatagcaat cgcctgacca tcccaactgc ttcattttgg
    46141 atttatggaa ccattctagt cttatatttc gcagtttgct accctatttc caaactatcc
    46201 actcacttag aaaaacattg gagaaactaa atgtctgaaa ctatcttaga aatcaaggaa
    46261 ctaaaaaaat ccttcggaga caatcccatc ctccaaggac tttctctaga aatcaaaaaa
    46321 ggggaagttg ttgtcatcct agggccattt ggttgtggga aaagtaccct ccttcgttgc
    46381 ctcaacggct tagaaagtat tcaaggtgga gatattcttc tggatggtca gtctatcgtt
    46441 gaaaataaaa aagattttca cctagttcgc caaaagattg gcatggtctt tcaaagttat
    46501 gaactctttc cccatctgga tgtcttacaa aacctcatcc taggccctat caaagctcaa
    46561 ggaagggaca agaaagaagt aacggaagaa gctttgcaat tactagatcg tgtcggttta
    46621 ctggataaac aacatagctt tgctttgccc gtcaattatc tggtggacag aagcaacgtg
    46681 ttgcaattgt ccgtgccctc ctaatgcatc cagaaatcat cctttttgac gaggtgactg
    46741 cttcgctgga tccagaaatg gtgcgtgagg tgctggaact tatcaatgat ttggcccaag
    46801 aaggccgtac catgatttta gtaacccacg aaatgcagtt tgcccaagcc attgctgacc
    46861 ggattatctt cctcgaccaa gggaaaatcg ctgaagaagg aacagctcaa gccttcttta
    46921 ccaatccgca aaccaaacga gcccaggaat ttttaaacgt ctttgacttt agccaattcg
    46981 gctcatatct a
//
LOCUS       DAVBDG010000008        44576 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBDG010000008 DAVBDG010000000
VERSION     DAVBDG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.008"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..354
                     /locus_tag="VQ526_000420"
     CDS             <1..354
                     /locus_tag="VQ526_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2554189.1"
                     /translation="KPNEEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEES
                     PEESEEPQVETEKVKEKLREAEGLLGKIQNPIIKSNAKETLTGLKNNLLFGTQDNNTI
                     MAEAEKLLALLKESK"
     gene            776..1156
                     /locus_tag="VQ526_000421"
                     /pseudo
     CDS             776..1156
                     /locus_tag="VQ526_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
     gene            1614..2327
                     /locus_tag="VQ526_000422"
     CDS             1614..2327
                     /locus_tag="VQ526_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2554190.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2341..2988
                     /locus_tag="VQ526_000423"
     CDS             2341..2988
                     /locus_tag="VQ526_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554191.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSN
                     FDDFLEKNYLKTIWQVSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            3126..3779
                     /locus_tag="VQ526_000424"
     CDS             3126..3779
                     /locus_tag="VQ526_000424"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2554192.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTSWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3789..4253
                     /locus_tag="VQ526_000425"
     CDS             3789..4253
                     /locus_tag="VQ526_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2554193.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4266..5534
                     /locus_tag="VQ526_000426"
     CDS             4266..5534
                     /locus_tag="VQ526_000426"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2554194.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALFLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5548..5808
                     /locus_tag="VQ526_000427"
     CDS             5548..5808
                     /locus_tag="VQ526_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554195.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5819..7180
                     /gene="pdrM"
                     /locus_tag="VQ526_000428"
     CDS             5819..7180
                     /gene="pdrM"
                     /locus_tag="VQ526_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2554196.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7334..8302
                     /locus_tag="VQ526_000429"
     CDS             7334..8302
                     /locus_tag="VQ526_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2554197.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8318..9310
                     /locus_tag="VQ526_000430"
     CDS             8318..9310
                     /locus_tag="VQ526_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2554198.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9528..10571
                     /locus_tag="VQ526_000431"
     CDS             9528..10571
                     /locus_tag="VQ526_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2554199.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10635..12320
                     /gene="lpdA"
                     /locus_tag="VQ526_000432"
     CDS             10635..12320
                     /gene="lpdA"
                     /locus_tag="VQ526_000432"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2554200.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTSNDDDKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDCIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAIAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIVDKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12384..13373
                     /locus_tag="VQ526_000433"
     CDS             12384..13373
                     /locus_tag="VQ526_000433"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2554201.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13446..14516)
                     /gene="xerS"
                     /locus_tag="VQ526_000434"
     CDS             complement(13446..14516)
                     /gene="xerS"
                     /locus_tag="VQ526_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2554202.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETAGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14845..15797)
                     /locus_tag="VQ526_000435"
                     /pseudo
     CDS             complement(14845..15797)
                     /locus_tag="VQ526_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154109.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            15939..16628
                     /locus_tag="VQ526_000436"
     CDS             15939..16628
                     /locus_tag="VQ526_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2554203.1"
                     /translation="MFSGQRLKSLRKEKKMSQEKLGSLLNISKVAVCNWENAKSFPTM
                     DNLNELAKVLEVEPHYFEEHSEVISIYRQLNKANKNKVVVYSSELLEEQEKFDNKVVD
                     IFSYKTYKGLSAGTGYSYFGDGKYTLTYSTENIPHDFASWVVGDSMEPELPDGDVVLI
                     KQSTNIEDGQIYAIEFNNQTYVKKVYRYKTYLRLVSINEKYDDIYAPYEENPRIIGKI
                     VGHFTPIDDVK"
     gene            16747..18063
                     /locus_tag="VQ526_000437"
     CDS             16747..18063
                     /locus_tag="VQ526_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HET2554204.1"
                     /translation="MPYAIDLFCGAGGCSEGLIQSGFHILFSSDISEMVEKTYVNRHE
                     QLGLIQGKNTWFERRDIRELTGSDIKKHISELEIFKNKEIPEIDLIMGGPSCQGFSRA
                     GRRDKSDPRNMLFGEYVRVVNEIHPKYIILENVEGFMDMQFLGYKGITGIEYPDGSVT
                     PDILKSELNEIGYETLEPRILNAADYGVPQRRNRVIFIGYKKGLKQPQYPAPTHGPEN
                     YISLEEAIGDLIIDKEIKEMVNPSPSTYQNKSKEGRTPSLNGEPIASKKILNNEVSKQ
                     TDIVKERFSLFKQGETGTALRKRIFEEGIDLSGHKALIQLCCEKLNLSKQEVVSKFKD
                     KNVSKEYLDVLLTKKNIRQRWAPDQPSATIVTIADDYISPWENRTFTVREMARCQSFD
                     DSFEFLGKRTTGGLKRRVEVPQYTQVGNAVPPLLAKALADEILKVL"
     gene            complement(18138..18494)
                     /locus_tag="VQ526_000438"
     CDS             complement(18138..18494)
                     /locus_tag="VQ526_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554205.1"
                     /translation="MLWDAVYQADNFRNGISVGINGKSIRYVKRFTYSFVTLPSNKLK
                     KDGKDTFKTTSTSVLRVLNLSGGNYWGLPSKSGVASSVKELVNRNLHTGYNNLVINTI
                     GMAIPQLNSTFNYFKL"
     gene            18639..19535
                     /locus_tag="VQ526_000439"
     CDS             18639..19535
                     /locus_tag="VQ526_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554206.1"
                     /translation="MENPWIDTTSEIYNGEKIIVATADLKYIKKLLNSKKYPPVDKVD
                     DDANEKTKKAAKEKYKLRLNVYPQHFVGDIDNAKIIILSLNPGYSTEYYDAYKNSTNK
                     DGIKYEQTIKENLEMEQPFFHAFELANESDLGYWGNKMKCWVEDHDEKDNEKDKEKYN
                     KKIIKSLKKITKNIALAEFFPYHSISYNDMYDKLAKGTSPNSNRKIKDYLPTQKFLFR
                     KIKKRIDDKNDKVIIILTRSFAKWYEAIPELKNYENCFEVNNPNNPSLKPKNILKVTR
                     ISVESKINNLLNDLNKEVQTQE"
     gene            complement(20244..21794)
                     /locus_tag="VQ526_000440"
     CDS             complement(20244..21794)
                     /locus_tag="VQ526_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2554207.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLVVGIIVGSFRF
                     LIEKGFHLIQGLYQDQAHLFVNLFIIALFYLIICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMTLNWWGILWKKYILGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAGLLFVVEEVYHHFSRFFWVSTLAASLVANFVSLLIFG
                     LTPVLDMPDNIPLMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPHILGGGNQLVLSLTEQDFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGAPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(21810..22589)
                     /locus_tag="VQ526_000441"
     CDS             complement(21810..22589)
                     /locus_tag="VQ526_000441"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2554208.1"
                     /translation="MATIKEIKELLATVKDLDNPIFLDLEKDNRSGVQKEISKRKKAI
                     QAELDEDLRLEAMLSYEKELYKQGVTLIAGVDEVGRGPLAGPVVAAAVILPKNCKIRG
                     LNDSKKIPKKKHLEIFKAVQNQALSIGIGIMNNQVIDQVNIYEATKLAMKEAISQLSP
                     QPEYLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     AANAGYGTAKHLEGLEKLGVTPIHRTSFEPIKSLVLDKKES"
     gene            complement(22576..23427)
                     /gene="ylqF"
                     /locus_tag="VQ526_000442"
     CDS             complement(22576..23427)
                     /gene="ylqF"
                     /locus_tag="VQ526_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2554209.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIKIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGLNYFKEHY
                     PEKLAERFKQMKIEEEAPIIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGDD"
     gene            complement(23681..29098)
                     /locus_tag="VQ526_000443"
     CDS             complement(23681..29098)
                     /locus_tag="VQ526_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2554210.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVQPALPEAVVTDKGESEIQAELPVAVVSEKV
                     ESAVQPSLPEAIVTEKGEPEVQPVLPEAVVTDKGEPEVHEKPDYTQPIGANLVEPEVH
                     EKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYT
                     EPISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQI
                     VTSYEELHGKKISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNR
                     SATLSYHLVNTDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETT
                     MTYDRGKGEETATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDE
                     DVKKMYLKITSRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYV
                     HYIEKPVPKVDDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGH
                     LSSVDGKQYTIHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVE
                     DVKVTGNVVANNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNL
                     AKAYVEADIVANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGF
                     INSNLPFGKLEDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHS
                     YKRSRDKIKEISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKS
                     ERDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDID
                     KIMVHYADGTKEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKS
                     QLSSVELISQEVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLV
                     ENEDHQLNGDEAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDF
                     YGKTPSVIDRLINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDK
                     DINDWFIHSAKNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFL
                     ISTYNTMAYSSFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLK
                     SQNMVPTPVWDNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASI
                     FPNPNNDDKVLFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGM
                     LQTPDSSTTNGEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMML
                     LDHVEADAVLPKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTL
                     SSVNDLVDNNFMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSF
                     KHNAFRMWGYYGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWK
                     RHWYGEVLAKAKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSK
                     VFKALLKNTDGFFNKLFKEDI"
     gene            complement(29142..35621)
                     /locus_tag="VQ526_000444"
     CDS             complement(29142..35621)
                     /locus_tag="VQ526_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2554211.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     QVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVE
                     PEKVEPSKEYTGVQAGAIVEPEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPS
                     AEDTKPNNENTNTPEEMSIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHN
                     FINNSTDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVI
                     KLPETTGREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLKDATVEMEQEYANNTYGVQ
                     YQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVY
                     DELYRHKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENY
                     FMKVKSENFKDVMLPVTSITNDTKDNRDVYKIVASANSLIQHENNNVLENYTYYLPKT
                     QQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELPDGQESYVNNVFHGILVGT
                     NNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHA
                     DGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLID
                     KSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGNLNNVQRFANVGGVVGNLW
                     DPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGTYSNKNNKVVNVVQEDDEI
                     LTKDSTVQRGEVLEDAQIKEKKATFVSKNTIKTEDFNFSSRYVTDYKNLENADSSKEK
                     VYKNIEKLLPFYNRETIVKYGNLVETSSNLYNKELLSVVPMKDKEVISDINKNKSSIN
                     KLLLYYADNTSETLNVNYQTDFSNVAEYRIGGTNLIYTPNTLLRNYQNILDEVLPALN
                     SVEYKSEAIRKVLDVSKDVSLTELYLEEQFNTTKTNLKDSLTKLLTADAAIAENNNKV
                     IDNYVIEKIKNNKEALLLGLTYLERWYDFKYRDTKAKDLVMYHLDFFGKSNSSALDNV
                     IELGKSGYNNLLAKNNVITYNVLLAKNYKTNNLFDALEKYRKAFVPDKTNNEWFKEQT
                     KAYIVEEKSTIKEVSDKQSIAGSPYSIGVYDRLTSPSWKYPSMVLPLLTLPEKSVFII
                     ANISTIGFGAYDRYRSKEHPAGTDLNDYVEKKAKEAAVRFRDHYDYWYRILDDKNKEK
                     LYRSVLVYDAFRFGTDEKEDKDTYQATFETNHPAIKHFFGPAGNNVVHNSNGAYATGD
                     AFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRNGLGPEFYAKGLLQAPDHPN
                     DPTVTINSILKYDQSEESTRLQVADPTQRFGSVDDLNKYMHNMFDVIYMLEILEGKAV
                     AKLDTNQKYDLLRKIENEYKPDPDGNSVYATNVVRRLKPEELTKLTTFNSLIEHDIIT
                     RRGYVDEATYKRNGYYTINLFSPIYSALSSKIGTPGDLMGRRIAFELLAAKGYKDGMV
                     PYISNQYEKEAKAQGKVITSYGKQIGLVTDEIVLSKVFNNQYNSWIDFKKDMYKERED
                     KFGKLNKVSFIDPNGSWARQQKVTIDNINRLEKMIEDAVKFDAEDEVAKLYPETNSRV
                     LKLKKAIFKAYLDQTGDFRSSIFENKK"
     gene            complement(35830..37008)
                     /locus_tag="VQ526_000445"
     CDS             complement(35830..37008)
                     /locus_tag="VQ526_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554212.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(37178..40828)
                     /gene="addA"
                     /locus_tag="VQ526_000446"
     CDS             complement(37178..40828)
                     /gene="addA"
                     /locus_tag="VQ526_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2554213.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(40825..44100)
                     /gene="rexB"
                     /locus_tag="VQ526_000447"
     CDS             complement(40825..44100)
                     /gene="rexB"
                     /locus_tag="VQ526_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2554214.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            complement(44300..44455)
                     /locus_tag="VQ526_000448"
                     /pseudo
     CDS             complement(44300..44455)
                     /locus_tag="VQ526_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT        13270 a         8485 c         8406 g        14415 t
ORIGIN      
        1 aaaccaaacg aggagaaacc tcagacagaa aaacctgagg aagaaacccc tcgagaagag
       61 aaaccgcaaa gcgagaaacc agagtctcca aaaccaacag aggaaccaga agaagaatca
      121 ccagaagaat caccagagga atcagaagaa cctcaggtcg agactgaaaa ggttaaagaa
      181 aaactgagag aggctgaagg tttacttgga aaaatccaga atccaattat caagtccaat
      241 gccaaagaga ctctcacagg attaaaaaat aatttactat ttggcaccca ggacaacaat
      301 actattatgg cagaagctga aaaactattg gctttattaa aggagagtaa gtaaaggtag
      361 cagcattttc taactcctaa aaacaggata ggagaacggg aaaacgaaaa atgagagcag
      421 aatgtgagtt ctagttctca tttttttcat gaaaatgtgc aaaatatagt agattgaaac
      481 tagaatagta tacctctact tctaaaacat tgttagaaat cgatttgact gtcctgttct
      541 tatttcattt tactatatct taacagatag tgtaaataaa gataaactat ttactggcta
      601 attgatcagt taaacactag ttaaggagta atgatgaaaa aaagaacaat actattattg
      661 atggccagtc tgttagctct tgtcttagga gcatgtggtt tcttggacat attgatcctg
      721 gatcattctc atcaggatta ctctttactg ctattttaga aactggggtg gtttgatgga
      781 aagtattggt attggtcttg ttatcgtttc acattccaaa tacattgtag aaggtgttgt
      841 tgaactgatt agtaaagtag ctaaagatgt tccgattact tatgtaggag gaaccgaggg
      901 cggaggaatt ggaacgagtt ttgattaagt agatagggtt gtttccgaaa atacagcaga
      961 tactttactt gccttttttg acctaggttt tgctaaaatg aacttaaaaa tggtgactga
     1021 tttcagtgat aaaagtatca tcatcaacag ggttccaatt gtagaaggtg cctataatgc
     1081 agctgctctt cttcaggctg gtgcagaact gtcagttatt caaacacagt tagcggagct
     1141 tgaaatcaat aaataaggaa ttttactata actcttttta taggtaagct attgattatc
     1201 tcaactataa aatgttaagt aaatgatttc aaaatcagaa agggattgct ttatgcagtt
     1261 cctttttatt ttaacaggag tgaaactata gtgtttctaa attgtgaatc aatcaaaact
     1321 gattgtgatg gggctattct agctttagaa accttcaaaa attaaaattt aaggcaatca
     1381 attacttgga agagtatgaa agcatttagt ttataggaat tctaggtcta gaattacaca
     1441 tatatttatg aatacggggt gctcgatagt tagtattgtt ctattctgaa agatttgagc
     1501 tgtcagttgt atagaaagtg ttcgaatttt tttaagtgat taaattagtt aattgtatga
     1561 ggtgctttat gatataatgt tcttaatgaa ttttcagaaa ggaaaacctc aaattgttct
     1621 acaaatttct actcttcgac ctcgaccaca ctcttcttga ttttgatgct gctgaggatg
     1681 tggctttgac ccaacttcta aaagaagaag gagttgcgga tattcaggct tataaagatt
     1741 attacgttcc tatgaacaag gctctctgga aagacttgga gctgaagaaa atcagtaaac
     1801 aagagctggt taacacgcgc ttttctcgtt tattttctca ttttggacag gaaaaagacg
     1861 gtagttttct tgcccagcgt taccaatttt atctcgctca gcagggacaa acactatcgg
     1921 gcgctcatga tctcttggac agcctcattg agcgtgatta taacttgtat gctgcgacaa
     1981 atggcattac tgccattcag acaggacgtt tggctcaatc tggtctagta ccttatttca
     2041 atcaagtctt tatctcagaa cagttgcaaa ctcaaaagcc ggatgctctc ttttatgaaa
     2101 agattggcca gcaaattgct ggatttagta aagaaaagac gctgatgatt ggagattctc
     2161 taaccgccga cattcaaggt ggcaataatg cggggattga cactatctgg tataatcctc
     2221 atcacctcga aaatcacaca caagcccagc cgacttacga agtctattct taccaagact
     2281 tgctggattg tttagataaa aatattcttg aaaagatcac attttaaagg agattagcta
     2341 atgactacaa aaaaagctaa tattactatt gaagagtaca ttgaaatgtc tgaagttgat
     2401 tttaatgaag ctgttaatta tgaatttaca tctgacactt gtcaattagc aaatagtatt
     2461 tatcaatctc tttttaagtt ttttgataag aaaaatttct ctggcgattt aatttttact
     2521 tggaaatctc catcattagt caaagaaggg gattatattg ggagaaggga ttcacaagta
     2581 gataatctta gagtaatagg aaatatattt ccgaattatc ttactaatcg aaaatatagc
     2641 ctcaatatga atcgtaatgg ctgtatggga gattttcctc atgacttttt tgatatatac
     2701 ctagatcatg tagcaaaata tgcctacgaa caaaaagtta ataatattaa agagtattat
     2761 cctttaaaaa gagcgatttt acaccaagag aatgcattgt attttcgatt tttttctaat
     2821 tttgacgact ttttagaaaa aaattattta aagactatat ggcaagtttc taaagaaact
     2881 cctttttctg aaatggattt taatatgttt aaaaatattt cagaaaagat aatcttcgaa
     2941 agaggtagta aaatgcttaa tgatttaaaa agtaattata aaaaatagcc agtctttata
     3001 aactgtattc acgtattcaa atcgtatata tagtcgattt attaaatctt tagataaact
     3061 gtaaaaagtg gtttccatag ccactttttg ctataataga ggcagtaaaa atgaaggagt
     3121 cggctatgga acactcgtct tggcatgctt tgattaaggc gcaattacct gagggttatt
     3181 tcgggaaaat caatcagttt atggagcagg tctattctca ggggattatt tatccaccca
     3241 aggaaaaggt ttttcaggct ctcttgacaa cactgcttga agaagttaag gtggtaattc
     3301 tagggcaaga cccctatcac ggaccaggtc aagcgcaggg cttgagtttt tctgtacctg
     3361 actctatccc agctccacca tccttgcaaa atatcttgaa agaattgtca gatgatatcg
     3421 gagttaagaa atctcatgat ttgacatctt gggctgagca aggagtcttg cttcttaatg
     3481 cttgtttgac tgttcctgct ggacaggcca atggtcatgc tggtcaaatc tgggagcctt
     3541 ttactgatgc tgtgattcag gtggtcaatc atctagatag accagtcgtt tttgtactct
     3601 ggggagctta tgcacgtaag aagaaggcat tagttaccaa tcctcatcac ttgattatcg
     3661 aatcagccca tccaagtcct ttgtcggttt atagaggatt ttggggttcc aagccctttt
     3721 ccaaggccaa tgcattctta aaagagacag gacaagagcc aatcgattgg cttagataag
     3781 gagaaaatat gcctcagtta gcgacgattt gctacattga taatgggaaa gaactgctca
     3841 tgctccatcg taataagaaa cccaatgatg tccatgaagg gaaatggatt ggtgtgggtg
     3901 gtaagctaga gagaggagag acgccccagg aatgcgcggc gcgtgaaatc cttgaagaaa
     3961 cagggctcaa agccaagcca gttctaaaag gtgtcatcac ttttcctgaa tttacaccag
     4021 atttagactg gtacacctat gtttttaagg tgacggagtt tgagggcgac ttgattgact
     4081 gcaatgaggg gacgctagaa tgggttccct atgatgaggt tttgagcaag ccgacttggg
     4141 aaggtgacca cacctttgtt gagtggcttt tagaggataa acccttcttt tcagccaagt
     4201 ttgtttatga tggggataaa ttgttggata cccaagttga tttctatgaa taaaggagaa
     4261 agcagatgct actaatcaaa aatggtcgtg taatggatcc caagtctggt ttggatcaag
     4321 tttgtgatgt cttagttcaa gatgggaaaa ttatcaaaat tgcgcctgag atcacggaag
     4381 aaggagcaga aacgattgat gctactggtc ttgtggttgc tcctggtttg gtcgatattc
     4441 atgtgcattt ccgtgaacct ggtcaaacac ataaagaaga cattcatact ggtgccctgg
     4501 cagccgctgc aggtggtttt actactgtcg tcatgatggc taataccagt ccaaccattt
     4561 cagacgtgga gactttgcaa gcagttctcc agtcagctgc caaagagaag attaatgtca
     4621 agacagttgc gaccattact aaaaacttta atggtaaaaa cttgactgac tttaaggcgc
     4681 tcttagaagc tggtgcggct ggtttctctg atgacggtat tccgcttgag agcagtaaga
     4741 ttgtcaagga agccatggag gaagccaaaa aactcaatac ctttatcagc cttcatgagg
     4801 aagatccagg tttgaacggt gttcttggct ttaatgaaaa tattgctaga gaacatttcc
     4861 atatctgcgg tgctactggg gtggctgagt acgctatgat ggcgcgtgat gtcatgattg
     4921 cctatgcaac taaagcccat gttcacatcc agcatttgtc taaggaagaa agtgttaaag
     4981 tagtggagtt tgctcagggg ttaggtgcag aagtcacagc agaagtagcg ccacagcatt
     5041 tctctaagac agaagcactt tttttaacac aaggtagcaa tgctaagatg aatccaccgc
     5101 ttcgtttgga atcagaccgt cgtgccgtta tcgaaggtct caaatcaggt gtcatcacag
     5161 ttattgcgac tgaccacgcc cctcatcatg tagatgaaaa aaatgttgag gatattacca
     5221 aagcgccatc tggtatgact ggcttagaaa catccctgtc tctcagcttg acctatttag
     5281 tagaagctgg tgagttgagc ttgatggaat tacttgaaaa aatgacatac aacccagcca
     5341 agctttacaa ctttgaagca ggttacttgg ctgagaatgg tccagcagat atcactattt
     5401 ttgatgccaa ggctgaccgc cttgtggact cccattttgc ttccaaagca gctaattcac
     5461 cattcatcgg tgaaacctta aaagggcagg ttaaatatac catctgtaag ggacaaatcg
     5521 tctatcaagc ttgataggag ttagaatatg aattattttc gaaaacgtag ggagagacaa
     5581 gcgaaatcta atagtggaat ttactgtccc gcagctaatc gttccaaaat tcaatatgaa
     5641 actaaagaaa aagctgacca tgcggttcaa tatgataacg gaggtttagt tagaagctat
     5701 tattgtcgta catgtgcgtg ttggcatact acttcaaaat ctaataaacc tccgctaagt
     5761 ttgaaaaact atggtctgaa actctttggt ttagataaat cagaatagaa agagaagtat
     5821 gtataagaca aagtgtttac gagagaagtt agtattattt ttaaaaattt tcttcccaat
     5881 cctgatttac caatttgcca attattctgc ctcttttgtt gatactgcaa tgacaggtca
     5941 atacaacact atggacttgg ctggtgtatc tatggcaacc agtatctgga atcctttctt
     6001 tacatttcta acagggattg tgtcagcctt ggtgcctatc attggtcacc atcttggtcg
     6061 aggaaaaaag gaagaagttg cgtctgattt ttaccaattt atttatttgg ccttgggcct
     6121 atctgtggta ttgctgggga tggtactttt tttggcacca acaatcttga atcatattgg
     6181 gttagaagca gcagtagcgg cagtagcggt tcgctatctt tggtttttat ctatcgggat
     6241 tatccccttg ttgctcttta gcgtcattcg ttccttgctg gattcgctgg gcttgaccaa
     6301 actgtccatg tacctcatgc ttttgttact ccctctcaat agcggattta actatctctt
     6361 gatttacggt gcctttggtg ttccagaact gggaggggct ggtgctggtt taggaacatc
     6421 cttggcctac tgggtcttgc ttgggatttc tgttctggtt ttatttaaac aggagaagct
     6481 caaagcctta caccttgaga aacgaattcc acttaatatg gataaaatta aggaaggagt
     6541 tcgtttaggt ctgcctattg ggggaactgt cttcgcggaa gtggctatct tttcagtggt
     6601 tggcttgatt atggctaagt tttcgtcctt gattatagct agtcaccagt cagctatgaa
     6661 cttttcaagt cttatgtacg cctttcctat gagtatctca tcggctatgg ctattgtcgt
     6721 ttcctatgaa gtgggagcca agcgatttga tgatgcgaaa acctatattg gtctaggaag
     6781 atggactgcc ctcatttttg cggccttcac cttaaccttc ctttacattt ttaggggaaa
     6841 tgtggccagt ctttatggta acgacccaaa atttatcgat ttgacagtgc gttttttaac
     6901 ttatagtctt ttcttccagt tagcagatac ctttgcggcg ccgcttcagg gaattttgcg
     6961 ggggtataag gatacagtta ttccttttta ccttggtttg cttggttatt ggggcgtagc
     7021 aatccctgtg gctacgctat ttgattctct aacagatttt ggagcttatt cttactggat
     7081 cggcttgatt attagtttga ttgtgagtgg ggcgctctac cgttggcgtt taactgtgat
     7141 tatgaagaga tttgaatctt tagcaaaatc caaacgctaa aaagtttgtg aaaaaatatt
     7201 cttgataaga aaatcccttg ttttcacggg gttttctttt ttattttgtg aaatttcttt
     7261 tagaaaaact ttgacagtat ttgccaaaaa cggtaaaata gtaaggtaag aagaaagtta
     7321 gaaaggcaag aagatgtcaa ctttagataa aaatcttttg ctagaaatgt tccgtaagat
     7381 ggaagaaatc cgtcgcatgg acttaaaaat tgcacaatta gtaaagaaag ggaaagtgcc
     7441 aggaatgacg cacttttctg ttggtgaaga ggcagctaac gtgggggcta tgttagctct
     7501 caatccagat gatctgatta cctcaaacca ccgtggacac gggcaagcta ttgctaaagg
     7561 gattgacctc aacggaatga tggctgaaat ccttggtaaa tacactggaa cctgtaaagg
     7621 gaaaggtgga tctatgcata tcgctgacct tgatgctggg aaccttggtg ccaatggtat
     7681 tgtaggtggt ggtatgggga tcgctgtcgg tgcagccctc agtcagcaaa tgcaaaatac
     7741 cggtaaaatc gttgtctgct tctttggaga tggtgcgacc aatgaaggtg ttttccacga
     7801 agcagtgaac atggcttcta tctggaacct gccagtcatt ttctattgca ttaacaacgg
     7861 ttacggtatc tctgcggata tcaagaaaat gaccaatata gaacatatcc atcaacgtag
     7921 cgccgcttat ggaattcctg gaatgttcat cgaagatggt aacaatgtca tcgatgtcta
     7981 tgaaggattt cagaaagctg tggaccatgt tcgcagtggc aatggtccag tcttgattga
     8041 aagtgtaact tatcgctggc tcggtcactc atcatctgac cctggtaaat atcgtacgcg
     8101 tgaagaagtg gaattgtgga aacaaaaaga tccaatcgaa aacctccgca attaccttat
     8161 tgaaaataac attgcaagtg ccgaagaatt ggaagaaatc caagcgcaag taaaggaagc
     8221 agtagaagct tctgttaaat ttgcagagga aagcccattc ccaccgcttg aatcagcctt
     8281 tgaagatatt tacgcagact aaggagaaag agataaaatg gaaacaaaaa caatgtcctt
     8341 ccgtgacacc attatccttg ctatgtctga ggaaatgcgt cgcgatgaaa atgtgttctt
     8401 gatgggagaa gacgtcggtg tcttcggagg agacttcggt acttctgttg gaatgcttga
     8461 agaatttggt ccagaacgcg tccgtgactg tccgatttct gaagctgcca tctctggagc
     8521 agcagcagga gcagccatga caggacttcg tccaatcgtc gatatgacct tcatggactt
     8581 ctctgttatt gccatggaca atatcgtcaa ccaagctgct aaaacacgtt acatgtttgg
     8641 tggtaaaggt caggttccaa tgactgttcg ttgtgcagct ggtaacggag ttggctctgc
     8701 cgcccagcac tcacaatctc tagagtcttg gtttactcac attccaggac ttaaggttgt
     8761 ggcacctgga acacctgcgg acatgaaagg cctgcttaag tcttctatcc gtgataaaaa
     8821 cccagttatt atacttgagt acaagtcaga atttaaccaa aaaggggaag tgccagttga
     8881 tccagactac acaattccac ttggggttgg tgaaatcaaa cgccaaggaa cggatgtaac
     8941 agttgttact tatggaaaaa tgcttcgccg tgtggttcaa gctgctgaag aattagcaga
     9001 agaaggaatt tcagttgaaa ttgttgaccc acgtaccctt gttccgcttg ataaggatat
     9061 catcataaac tcagtgaaga agactggtaa ggttgttctg gtcaacgatg cccacaaaac
     9121 aagtggctat atcggtgaaa tttcagctat tatttcagaa tcagaagcat ttgactatct
     9181 agatgcacca attcgccgtt gtgcaggaga agatgtgccg atgccttatg cacaaaacct
     9241 agaaaatgca atgattccaa cagttgaaag tatcaaagat gcaatccgaa aaacttataa
     9301 caaagaatag agttacataa gataagttat gtaaataaaa taaagctgac gagaaacttt
     9361 gtatctttta ggtgcagagc tcttcgtcat taatatctta gattagagca cgggctaaaa
     9421 gctcggaaaa aagataaatc tccttggctt catcgaagcc atcgtcgatt tcctattttt
     9481 cagtcgcttt tgacgccctt tgtatcataa atagaattgg agaggtcatg gctgatgaca
     9541 agctaagagc gactcctgca gctagaaagt tagcggatga tttagggata aacctctacg
     9601 acgtttctgg ctcaggtgca aacggtcgtg tccacaaaga agacgtggaa acttataaag
     9661 acacaaacgt ggttcgcatt tcgccacttg caaaacgaat tgccctcgaa cataacattg
     9721 cttggcagga aatccaagga accggtcatc gtggtaaaat catgaagaag gatgttttgg
     9781 ccctgcttcc tgaaaatatc gaaaacgata gcatcaaatc tcctgctcag attgaaaaag
     9841 tggaagaagt ccctgataac gtaacaccat atggtgaaat cgagcgtatt ccaatgacac
     9901 caatgcgtaa ggttattgcc caacgcatgg ttgaatctta cttgactgcg ccaaccttca
     9961 ccctcaacta tgaagttgat atgactgaaa tgttggctct tcgtaagaag gttcttgagc
    10021 caatcatgga agcaactggg aagaagacta ctgtaacaga ccttctttcg cttgcagttg
    10081 ttaagactct tatgaaacac ccatacatca acgcttcatt gacagaagat ggcaagacta
    10141 ttatcactca caactatgtc aatcttgcca tggcagttgg gatggataat ggattgatga
    10201 cacctgttgt ttacaatgct gagaaaatga gtctttcaga actggttgta gcctttaagg
    10261 atgttattgg ccgtaccttg gatggtaaat tggctccaag tgagttacaa aattcaacat
    10321 tcacaatcag taatttggga atgtttggtg ttcagtcctt tggtccgatt attaaccaac
    10381 ccaactcagc tatccttggt gtcagttcga caatcgagaa gccagttgtc gtcaatggtg
    10441 aaattgtgat tcgcccaatc atgagtttag gattaaccat tgaccaccgt gtcgtagatg
    10501 gtatggctgg tgctaagttt atgaaggact tgaaagagtt gattgaaacg ccaatctcaa
    10561 tgttaattta agagcaagag tattcatttt taagatatag ataaaggaag gaagacatgg
    10621 ccttagaagt aattatgcca aaagccggcg tggatatgac agaaggacaa atcgtccaat
    10681 ggaataaaaa agtcggagaa tttgtaaaag aaggagaaat ccttttggaa atcatgactg
    10741 ataaagtcag catggaattg gaagccgaag aagatgggta cttgattgcc attctcaaag
    10801 gagatggtga aactgtccct gtaacggaag ttatcggtta ccttggtgaa gaaagggaaa
    10861 acatcccaac agctggagca gcatcgccag aagctagctc tgtacctgta gcaagtacct
    10921 caaacgatga tgataagagc gatgatgctt ttgatatcgt tgtgattggt ggaggtcctg
    10981 ctggttatgt tgcagccatt aaagctgccc aattcggcgg taaggttgcc cttgttgaga
    11041 aatctgaact tggtggaacc tgcttaaacc gtggatgtat tccaaccaag acctaccttc
    11101 ataacgctga aattattgaa aatatcggtc atgctgcaaa tcgtggtatc gtgattgaaa
    11161 atcctaactt cactgttgat atggaaaaac ttttagaaac taaatctaaa gttgttaata
    11221 ctcttgttgg tggtgttgca ggacttcttc gtagctacgg agttactgtt cataaaggaa
    11281 ttggtacaat cactaaagac aagaacgtct tggtaaatgg ctctgaattg cttgaaacca
    11341 agaaaatcat tcttgctggt ggttcaaaag tcagcaagat caacgtccct ggtatggaat
    11401 ctccacttgt catgactagt gatgacattc ttgaaatgaa cgaagtgcca gaaagccttg
    11461 ttatcatcgg tggtggagtt gtcggtatcg aactcggtca ggccttcatg acatttggtt
    11521 caaaagtgac tgttatcgaa atgatggact gtatagttcc agctatggat gcggaagttt
    11581 ctaagaacct tcgcttgatc cttgagcgta aaggaatgac catcttgact ggtactaaac
    11641 tgcaagaaat cattgaggaa aatggtcaac ttcgtatcaa ggttgaagga aaagacgata
    11701 tcatcgcaag caaagctctt ctttcaattg gtcgtatgcc agaccttgaa ggtattggag
    11761 aggttgagtt tgaattggat cgtggttgta tcaaggtcaa tgaatacatg gaaacttcag
    11821 ttccaggcat ttatgcacca ggtgacatca acggtactaa gatgttggct cacgcagctt
    11881 tccgcatggg tgaagtttcc gctgaaaatg cccttaaagg aaatcatgca attgccaaat
    11941 tgaatttgac tcctgcagcc atctacactc tccctgaagt agcagcagta ggtttgacag
    12001 aagaacaagc ccgtgagaaa tacgatgttg ccatcggtaa gtttaacttt gctgctaacg
    12061 gtcgtgctat tgcatctgac gctgctcaag gtttcgtaaa agttatcgtt gataagaaat
    12121 acggagaaat ccttggtgta cacattattg gtcctgcagc cgcagaatta ataaacgaag
    12181 catcaagcat catcgaaatg gaaatcactg ttgaggaaat gctgaagacc atccacgggc
    12241 acccaactta ctctgaagtg atgtacgaag cgtttgcaga tgttctagga atggccatcc
    12301 attcacctaa gaaaaaataa aacgaagata gattagactg gaaggttatt tccagtttct
    12361 atcatataaa gtaaagggat atcatgaaat acattatcaa tcattcaaat gacactgctt
    12421 ttaatattgc cttggaagaa tacgccttta aacacctttt ggatgaggat caaatcttcc
    12481 tactttggat taacaagcca tctatcattg ttggtcgtca ccagaacact atcgaagaaa
    12541 tcaaccgtga ttatgttcgt gaaaatggta ttgaggtagt tcgccgtatt agtggtggtg
    12601 gagctgttta ccacgattta aataacctca actacacgat catctccaaa gaagatgaaa
    12661 ataaggcatt tgacttcaag agcttctcaa ctccggttat caataccctg gctcaacttg
    12721 gggttaaagc tgagttcaca ggtcgtaatg accttgagat tgatggtaag aagttctgtg
    12781 gcaatgccca agcctatatc aacggccgta tcatgcacca cggttgcttg ctctttgacg
    12841 ttgatttgtc agtcctcgca aatgccctta aggtttcaaa agataaattt gaatcaaaag
    12901 gtgtgaaatc cgtccgtgcc cgtgtaacca atattatcaa tgaattacca aaaaaaatca
    12961 cagtcgaaaa attccgtgat ttactcttgg aatacatgaa aaaagagtac ccagagatga
    13021 ctgaatacgt cttttcagaa gaagaattgg ccgaaatcaa tcgcatcaag gatactaagt
    13081 ttggaacttg ggactggaac tatggtaaat cacctgaatt taacgtccgt cgtggaacca
    13141 aattcactag tggtaaggtt gaagtctttg ctaacgttac tgaatcaaaa atccaagaca
    13201 tcaagattta tggtgacttc tttggtattg aagacgttgc tgcagtagaa gatgtccttc
    13261 gtggggtgaa atacgaacgc gaagatgttc ttaaggcgct aaaaaccatt gatatcacac
    13321 gctacttcgc tggtattagc cgtgaagaaa tcgctgaagc ggtagttgga taaatcaaaa
    13381 agaagttggc caactaacca acttcttttt ttgatattta tttacataat ttaaaatacg
    13441 taaaattata aactatctag agcattcttt tgttcatcat taacaatatg ggtatagagg
    13501 tcagtgactt gtgtgctggc atgtcctagt tggtgactga ctaaaacttg tgatttagtc
    13561 gcatcataga gcctagttgc tagcgtatgg cgcagtttat ggggtgttac acgcacttta
    13621 aaatcctctg agtatttagc aaccattttc tcaacgctag aagcatcgat acgattagga
    13681 acacctctgt agagagttaa aaaaagggct gtatctgttt tttccgtttt atagcgttga
    13741 ttccgaatgg ccagataatt ctctaaataa ggtttagcaa aagcagcgac attgactgag
    13801 tcacgtttgc caccttttcg agtaacatca ataaccatca tttttagatt gagatctctt
    13861 agatctagat taacagcttc agataagcga acaccagatg ccaagagaag ggcaataatg
    13921 gctaaatctc gttctttatt tttgttgaat gatgagagag ctcgatttga aagctgttgt
    13981 gggtgctctt gatcgatata agttagaaaa cctgctgttt catcacctag aaagagtttt
    14041 tgcttgatat tttcagctct ggcagcaagg gtttctttct ttttcttggt tgaaactttt
    14101 ttcattacat tacgatagaa ataaggttcc ccctgatcat tttcaacctc ctcggttaga
    14161 tacttgtaaa gactagaaag tgctgataag gttcgattga tagttgtctg tgaaacacct
    14221 tgttttgttg tattagcatt cagcaaggga cgttcacgta gataaaggat aaaggattcc
    14281 atgtctttct tagacatatt ttccaaaact gataaaggaa tatcggatat tttatcagcg
    14341 tttgaaatac cagactccaa aacccagctg aaaaatcggt catattcctt aaggtattcg
    14401 tacagggttg taaaactgta gggcacagcc agtttagatt ggtagtattc cagaacatac
    14461 cagggcatga gttgttttag tttgtcgatt cgttccagta aaatctcacg tttcatgtat
    14521 tttctccata attaagtata ctagtagtat agcatagact tgtatatttt tcaagaaaat
    14581 tatagttttt cagaaagatg ttatagagct ttttaaaaaa gacttagacc tgagtaatac
    14641 ggatctaagt cagctaaata actaatactc ttcgaaaatt aaattcaaac cacgtcaatg
    14701 tcgccttact atactcaagt acaaagtaca gcctgcggct agcttcctag tttgcttttt
    14761 gatttttatt gagtataaaa aaattttttc gttcaaaatt gcctaaaatg taccttatta
    14821 aagtgaaaat taaaaatttt tcatacgaaa tcgttgatag tatacatcgt atgataatgc
    14881 ttttcctcgt ctattaagat ttaatggtcc atagaccttc tcatacttct taccttccag
    14941 ataagtcata tagtcattgt aggtgtctaa aaagacctct aagaagatat aaggtactgc
    15001 ttgaagtctt tccttttctt ttcaattcgt ccagttggct ttaaatagct tctaagaggt
    15061 atttcttttc gtaagtcaat ttcaatttta aatacttggc catcactttg gtgaaataaa
    15121 atacctggcc ctagaggact atcttcacgt cttacgttgc atgcttctga tggtcttaaa
    15181 ccaacaaagg ctcccaaggc tacaaccata agtagatctt tatgatagtg tgcaatgtga
    15241 gaaaacaaca tctcaaaggc gctatttggc atatcacgaa aaatagcttt ctcagtgttg
    15301 ctatcatcca cgtagatttc aaaatttggt tctttacgct ttacaactcg tcctctacta
    15361 ttcgtaaaag tcgtcgtaga atacaattct tctactttta acttagcctt ctcctttcgc
    15421 tcacttaaat aaagagttaa gaagtcaaga actgcattta cgcatttttc aacagtgctt
    15481 ttctttcttt ttcctctatc ccctggtaat tgagataatc cataaagcgt aaaaaactct
    15541 ctaaccattt caagtgtaag ttgatctaca cttttaagtc caagagtacc aaatataaaa
    15601 tttagaaact taacaacata agaaaagcat ttattcccat catctgagat cgacctgatt
    15661 tttgaagcag atttaacata acgatgaaaa tcagtaaatt gtagattgcc gtcatcgtgc
    15721 tttaaaacga taaattgttt tgttacgatg ttgttgtcac ctaaaaccag cgaatgttta
    15781 taaactacaa atttcatttc ttctccattt gttaattata taattaacta tttgatttta
    15841 ttataacttt ttgatagaat tcttgtaaag tgaaaactta acaaaaagag aaaaataaaa
    15901 aaagttaatt aacaaaagta aaggaaaata agtcatacat gttttcaggt caacgtttaa
    15961 aatctcttcg aaaagagaaa aaaatgagcc aagaaaaact cggctctctg ttaaatattt
    16021 ctaaagttgc agtctgtaac tgggagaacg caaaatcttt tccaaccatg gataatctaa
    16081 atgaacttgc taaggttctt gaggttgaac ctcattattt cgaggagcat agcgaagtga
    16141 tcagcatcta tcgtcaatta aataaagcta acaaaaataa agttgtcgta tattcctcag
    16201 aattacttga ggaacaagaa aaattcgata acaaggttgt tgatatattc agttataaaa
    16261 cctataaagg tctttctgca ggaacaggct actcctactt tggagatggg aaatatactc
    16321 ttacttattc aactgagaat attcctcatg attttgcctc atgggttgta ggtgactcaa
    16381 tggaaccaga acttcctgat ggtgatgtag ttttgattaa acaatccaca aacatcgagg
    16441 atggccaaat ttatgcgatt gagtttaata accaaacata tgtaaaaaaa gtatatcgtt
    16501 acaaaacgta tctacgactt gtttcaataa atgagaagta tgatgatata tatgctccat
    16561 atgaagaaaa ccctcgaatt attggaaaga ttgttggtca ttttacacct attgatgatg
    16621 tgaaataaga ttatattaat gataaagaat tatatttaaa aaggttttaa gtggaaagat
    16681 tctttttcct ggcctttttt aaaaaaataa attataatag agaaaatact tagaaagagg
    16741 ttcttaatgc cgtacgcaat agatttattt tgtggtgcag ggggttgttc ggaaggatta
    16801 atccagtcag gttttcatat tttatttagc tcagatatca gtgagatggt agaaaaaact
    16861 tatgtaaata gacacgaaca acttggattg atacaaggaa aaaatacttg gtttgaaaga
    16921 cgtgacattc gagagttgac aggatcagat ataaaaaaac atatctctga attagaaatt
    16981 tttaaaaaca aagaaatacc tgaaattgat ttaatcatgg gaggtccaag ctgccaagga
    17041 ttttcaagag ctggtagaag agataaatcc gatcctcgca atatgctttt tggcgaatat
    17101 gtgagagttg tcaatgaaat tcatccaaaa tatattattt tagaaaatgt tgaaggattt
    17161 atggatatgc agttcctagg gtataaaggg attacaggta ttgaataccc tgatggatca
    17221 gttacacctg atatattaaa gtctgagtta aatgaaatag gatatgaaac tttagaacct
    17281 agaatattaa atgctgccga ttatggggtt cctcaaagac ggaatagagt tatttttatt
    17341 gggtataaga aaggtcttaa acaacctcaa taccctgctc ctactcacgg tccagagaat
    17401 tatatttcac ttgaagaagc tatcggagat cttattattg ataaagagat caaagaaatg
    17461 gttaacccat ctccttccac ttatcaaaat aaaagcaaag aaggtaggac accaagtcta
    17521 aatggagaac caatcgcttc taaaaagata ttgaataatg aggtatcgaa acaaactgat
    17581 attgtaaaag aacgcttctc gttgtttaag caaggtgaaa ctgggaccgc tttaagaaaa
    17641 agaatttttg aagaaggcat tgatttatct gggcacaaag cgttaattca gttatgttgt
    17701 gaaaaattaa acctttctaa acaagaggtc gtttctaaat ttaaagataa aaatgtaagt
    17761 aaagaatatt tagatgtcct tcttactaag aaaaatataa gacaacgttg ggcaccagat
    17821 cagccatcgg ctactattgt aacgattgct gatgattata taagcccttg ggaaaatcga
    17881 acttttacag ttagggaaat ggcacgctgt caatcttttg atgattcatt tgaattttta
    17941 ggaaaacgta caactggtgg gctcaagaga cgggttgaag ttcctcagta tactcaagta
    18001 ggtaacgctg taccaccact cctagcaaaa gcattagctg atgaaatact gaaagtttta
    18061 taatttagca tcataaaaac aaaaacttat aatcagaatt aaactatttc tgattataag
    18121 ttttttatgt tataaattta taatttaaaa taattaaatg ttgaatttaa ctgaggtata
    18181 gccataccaa ttgtattaat aacaaggtta ttgtacccag tatgtaagtt tctattaact
    18241 aattctttaa ctgagctagc aacaccgctt ttagaaggaa gaccccagta atttccacca
    18301 gaaagattta aaacacgtaa aacggacgta gaagtagttt taaatgtatc tttaccatct
    18361 tttttaagtt tgttggatgg taatgtaaca aacgaatagg taaatctctt tacataacga
    18421 attgatttgc cgtttattcc aacagatatg ccatttctaa agttatcagc ttggtatact
    18481 gcgtcccata acataggaat ttgagcatta tcattccaat ttgttttgca ttgaatgatg
    18541 tgaacttcta tatcattaaa atcacgttcg acgagagcgt taattatttc ttttttatga
    18601 taaaatagtg ttattaattt gaattagagg ttattgtgat ggaaaatcct tggatagata
    18661 ctacttcgga aatatataat ggtgagaaga taatcgttgc tactgcggac ctgaaatata
    18721 ttaaaaagct attaaattca aaaaaatatc ccccagtaga taaagtagat gatgatgcta
    18781 atgaaaaaac aaagaaagca gccaaagaaa aatataaact ccgactaaat gtttatcctc
    18841 aacattttgt tggtgatata gataatgcaa aaattataat cttaagttta aacccaggct
    18901 atagcacaga atattatgat gcttataaaa acagcactaa taaagacggc attaaatacg
    18961 aacaaactat aaaagaaaat ctagaaatgg aacagccatt cttccacgca tttgaattag
    19021 caaatgaaag tgatctagga tattggggaa ataaaatgaa atgctgggtt gaagaccatg
    19081 atgaaaaaga taatgaaaag gataaagaaa aatataacaa aaaaattatt aaatctttaa
    19141 aaaagataac taaaaatatt gcacttgctg agttctttcc ctaccattct atatcttata
    19201 atgatatgta tgacaaatta gctaaaggta cttccccaaa tagtaacaga aagataaaag
    19261 attaccttcc tacacaaaaa ttcctcttca gaaaaattaa aaaaagaata gatgacaaaa
    19321 atgataaagt gataataatt cttactcgtt cctttgcaaa atggtacgaa gcgatccctg
    19381 aattgaaaaa ttacgaaaat tgttttgaag taaacaaccc taataatcct tctctaaaac
    19441 caaaaaatat cttaaaagta acaagaatat cagttgaatc aaaaattaac aatttattaa
    19501 atgaccttaa taaagaagtt caaacacaag aatagtagct atcaatgaaa tagctacttt
    19561 atttttaaaa atgtacaatt gaaccaccac ttcttctgct gttacttgag taaaaaatgt
    19621 acaaatcaat ttcttagatt tgaggatatt atgtactata aaacacacaa aataccactc
    19681 cagaataatg tacaaaacaa aaataaagta cacttttcaa agaatgtact ttatgcttct
    19741 aattcagttg tatcaatggt ttagctttag tttaatcaat tgtacataaa cttttagttt
    19801 ttgagtaaaa cagatctaaa tctttggagt ttcattgtga atcattatct aatgcttttt
    19861 ctagtttcca aatactaaac cagaatgaaa tagtcagcag aataaggaaa ataaaaaaga
    19921 gattatcagt aacgatactt ctgttcattc tacataactg aagagctata taaggagcta
    19981 tgagatacaa aatgcattgt ataattgcta tcgttttctt tttcatgatt ttaatcttac
    20041 tgtacagatt tgatacagta aactcctttc tagcttgatg cattatcatt tctaacttga
    20101 ttatagtctt atttttacca aagttcaatt atttgtatga gaaatatctt atctgtaaaa
    20161 aataaccgat ctcattgctg agaatcggtt atttaaataa attaagttgt tatttaaata
    20221 acataaatta tgtaacaatt atactacaac aacaaatctt tgacttttcc aatttcactt
    20281 tttggaatca ccaggtgaat catatcaccc agatacattc tggttgagcc gttaactgtt
    20341 tggctcttgc cattatggac ttgggtagtg atgaggacgt tgtgtggtaa gttgagttca
    20401 tgaacttgtt tcccagcaat tttatcagaa actggtattt cgataagtgt aacttctcct
    20461 tcgctggata cttcttctgg aagcattttt tccagcatgg cttcatagac tggtgcaccc
    20521 ttgagtaaat ccatgataat ataagaaaca agagtgacaa gaccaagtgg catgaggttg
    20581 cgaatatctc ctaccatctc agttacgagt atcatagcag ttaagggagc cttagatatt
    20641 gctccaaaat aaccactcat tcctagaatg acaaatatag ggaattgctc ttgactgaca
    20701 agtccaagat tgacacagat aacaccaact aaggcaccaa gcaaggaacc gagagccaaa
    20761 attggtagga aaattcctcc tggaagacca cttccatagc taatcatgct ccagataaaa
    20821 cggatcaaaa agtaagctaa tagaacttga aaactaaaat cttgctcagt taaggaaagt
    20881 acaagctgat ttccaccacc aaggatgtgg ggtaaaaaga ttccgactgg tatgatgaga
    20941 atgaaggcca gaattggata atatgctcta tccaaatgga ttttttgacc aatcaagtca
    21001 taaactcttc cgacgtttaa tacagctttc tcatagagaa aacctgaaaa tccaaggaaa
    21061 attcccataa cgagatatat ccaatactga tctagggtca ttagagggat attatcaggc
    21121 atatccagta cgggtgttag gccaaatatg agcagagaaa caaagtttgc tacgagactg
    21181 gctgctagtg tagaaaccca gaaaaagcgt gaaaagtgat gatagacttc ttctaccaca
    21241 aagagaagtc cagcaatcgg tgcattaaag gcagcggcta gtcctgctgc agcaccactg
    21301 gcaatcaagg aacgttcctc cactggactg gatttgagcc acttggcaat tcctttacca
    21361 ccaactgctc ccagttggat acttggccct tcacgcccca gcatgagtcc gctggcaata
    21421 gctagaattc ctagaatata ttttttccag agaatgcccc accagttgag ggtcatgagg
    21481 cctttcagtt cggcttcgac ttgcggaatt cctgagcctt taatatcttt ttcagagcgt
    21541 gtcagtttgg cactgagcca gcagatgatt agataaaaca gggcgataat aaataaatta
    21601 acaaataggt gcgcttgatc ttgataaagt ccttgtatca ggtggaagcc cttttcaatt
    21661 aagaaacgaa aggatccgac gataattcca acgacgaggc cgacaatgat tcctcgacca
    21721 acttgggata gtatagtgct ggatgcaaag gcgaattctt tcttagaacg gactatttcc
    21781 gactgttcct ccatggtatc tccttttaat taactttctt ttttatctaa aaccagtgat
    21841 ttaattggtt caaagctggt tcggtgaatt ggggtaactc ctaatttttc gagtccttct
    21901 aggtgtttag cagttccata tcctgcattt gcagcgaaat catagccagg gaactgctga
    21961 tcgtattcct tcatcaattc atcacgtgtc accttggcta ctatggaagc tgctgcaatt
    22021 gaaagggaat tggcatctcc tttgatgatg gaagtttgtg aaattggtaa atccagtttc
    22081 atggcatcta tcaaaaggta ctcaggttga ggactgagct gggagattgc ttccttcata
    22141 gctagtttgg tcgcttcata gatattgact tggtcgatga cttgattatt catgatacca
    22201 attccgattg acagggcttg gttttgaacg gctttgaaaa tctccagatg tttcttttta
    22261 ggaattttct tgctgtcgtt gagacctcta attttacaat ttttaggtaa aataacggct
    22321 gcagcgacta caggtccagc cagaggacca cggccaacct catcaacacc agcaattaag
    22381 gtcactcctt gcttgtaaag ttctttttcg taggaaagca tagcttccaa acgaagatcc
    22441 tcatccaatt cagcttgaat ggctttttta cgcttgctga tttccttttg aactccagag
    22501 cgattgtcct tttcaagatc taaaaaaata gggttatcaa ggtccttgac tgtagcaagt
    22561 agttctttga tttctttaat cgtcgccatc taggtcttcc aatgtatcta aggtatagtt
    22621 accgagtttg ccatcacgga cttccttcac gaagagactg tagaaacggt catagtcgtc
    22681 tctgaaaccg agggcgcggg tcatgtccat aataataatc ggagcttctt cttcaatttt
    22741 catttgtttg aaacgttcag ccaacttttc tggataatgt tctttgaaat aattgaggcc
    22801 aaaaatagtt acctcatcca taggaagcaa ctggtctttg atagctccag tcaaggccag
    22861 tttcagtgca acagtttcat cttcaaactt aggccaaaga atccccggtg tgtctaggat
    22921 ttcaaggtcc ttattagttt tgagccactg ttgtcccttt gtaacccctg gtttgttgcc
    22981 gacaacggca attttcttgc cagccaaacg attcatgaga gttgatttgc cagcgtttgg
    23041 aatcccgata atcatggtac gcaaggtttc aatcttaatc ccacgttctt tctggcgagc
    23101 aatcttatca gccatgagtt ttttagcagc atctgttaca acttttacag tcacttgctc
    23161 cttggagttg atagccagag tttggattcc ttgtgattca aaatactgac gccattcctt
    23221 ggtcattgct ggatcagcca aatccgcctt atttaaaatc aagagttttg gtttatcacc
    23281 aacaatcttg gtcaacatag gattttgact agatagaggt aagcgtgcat ccaccaaaat
    23341 cgttacaaaa tcaacaaatt ttaaattttc ctgcacctgt cgacgcgctt tagacatgtg
    23401 accaggaaac cattgaatag tagccattga gtttttttcc tttcgtagca agggtttaga
    23461 gatcctattt tattttacta tcgtttgaac ataaagggtt ctcaaatgaa tgatgttcaa
    23521 acagtttttt gttttatgac atagaaatct accttatatt atatcatgcc ttctttattt
    23581 tgtccgatga tagcctttca agtaccctat atagaaaaaa gccatcaaat gatgactttt
    23641 cagagggagc aaaaaactgt tccttctata ttctaaacat ttaaatgtct tctttaaaca
    23701 gtttattaaa gaaaccgtct gtgtttttaa gaagggcttt gaagacctta gatttaagag
    23761 caacagtgtg agagaagtca tctggtttct tcaaatcttc ttcaactgct ttatcaaata
    23821 aaggccttaa ctcatcatag ttactgatat ggacgccatc aatatcgata gcctcaaaac
    23881 ctttcttagc tttagcaaga acttccccgt accaatgtct cttccactct tcaagagtat
    23941 taaagtttcc tttcgatact ttattaatta ttagcttatc gcttagtaaa ccgtggttgt
    24001 ttttatctgc ttctgctttg tatttatttg atacataact aataaagcca ttttcataac
    24061 cataatagcc ccacatacgg aaagcattgt gcttaaatga taatgaacca ggagcacctt
    24121 gactcgtatt cccaccgtaa attcctgcca tcatattgac atttacgtaa gctgagtttg
    24181 gtgtaaaatc ttctggtcta taacgtccat tacctggatt tccatgtttt gtcatgaagt
    24241 tattatccac taaatcattg acgctagata aagtcattac cttttcttca tctttcaaat
    24301 cacggatact atcccattgg tttggaccta aaagatcatt atactgaatc ttagagcgca
    24361 tttctttgtc aatcttcttg aaccacttac tattgtctcc tttaatttta ggaagtacag
    24421 catcagcttc tacatgatca agcaacatca tagcttcatt gtagtttctc atgtagcggt
    24481 caatgtcatc acgtgtcttt aatttgtcag gatctgggtt gtaccattgg ttaccatcat
    24541 ttggacgatg gtaagccatg ttaattccaa gtgcgccgta ttcaccatta gtcgttgagc
    24601 tgtcaggtgt ttgcaacata ccttgagcat aggcttcaac gtctgtacct tgacgatgct
    24661 tatgaccacc gaagtaaagc attctatcgt ttacgtgtgt tgtttcgtga gtaaaggctg
    24721 aaataccaaa tgggctaatc atatccgtca ccataaacaa gaccttgtca tcattatttg
    24781 gattagggaa tattgatgcc atagcgccca tacgccagtc tctatcatgg tgtcgccatg
    24841 ttggaccata taattcacga attggagcta tagggttacc tttactgtca aatcctcgac
    24901 gacctacagg agaaccattg taggtctggt tatcccaaac tggtgtcggt accatatttt
    24961 gactcttaag aagtctatca cgaacattag gcagagctaa tctggaccag aaatctaaat
    25021 aagtttgttg agcatgtgca acttcatcaa tcttagtttt gaactcatta cgagcttctt
    25081 ctgtatgttt tccatatttc tcaaatgaac tgtacgccat cgtgttatat gttgaaatta
    25141 agaacatatg ggcatcttta agcgttagaa gtggtaaaat catacgacca tgaacaccgt
    25201 tatctaaacc atcgaacaca cgatgacgtt tatctttcaa ctctgtattt gttgtcttag
    25261 gttctgaaac ataaacattc ttagctgagt ggataaacca atcattgatg tctttatcct
    25321 ctgtaaacag acgcatgttg tattctaaga agttacgcaa attactttta ccagtattac
    25381 ctgcgatgat ttcacggtaa gcatcctgag tccgatcccc tttcaagttc ttttcacttg
    25441 aaccgatgtt gatgagtctg tcaataacac ttggtgtttt accataaaaa tcaggtttga
    25501 acatcatgat gttttttata cttatgtcac cgtatttgat atcatagtaa cgattgagat
    25561 aagtcagagc catcatgatt ttagctttat tggtttccac tttcttgaga agtgatttta
    25621 tcgcagcttc atcaccattc aattgatggt cttcattttc gaccaaggat ttgactaact
    25681 tatctagatt ttgcttcact tcggcaaagc tttcttctaa gtatagatct ttaatataac
    25741 cattcttacg ttcatccgta ttctctgcag gtttatcacg tctactcatt aaatctctta
    25801 cttcttgtga aatcaattcc acacttgata attgagattt aacatcagca atcaatttat
    25861 cacgattttt aacaaccata ttcggtgtat aaacaacatc accaagacca gatacactat
    25921 attcacgcac ttgttggact ttagaatcag tattcttagt aacatccatt tcttccttag
    25981 taccatcagc atagtgaacc ataatcttat caatatctga taaatcggtc acgaattgac
    26041 catccttcat ccccgtaaca gaaaggactt ctttcttagc taagtttgaa tcttctgcaa
    26101 gtttgttacc ttggttgaca atccattctt tattataaaa tggttgaagt ttctcaatat
    26161 tacgataagc taaatcacgt tcggatttgt agtcttgagt tgacttatat tcatcttctc
    26221 ttctggttaa acgatttaag cgattgacca caggttcgtt gatttcgtat ttatcagcag
    26281 taagtccaaa atttgcaatt ctcttattag cttcatcttg actaatttct ttaattttgt
    26341 cacgagaacg tttgtatgaa tgcttacctg aactgacacc tttgacaaca aagttacgct
    26401 ctaaccagcc atttgtgaaa taaccaccat cgtagtccaa atcggaagaa ccaaagaact
    26461 cttcaccatt ttcaactctc atcattgaaa tattatcttc aagtttacca aaaggaagat
    26521 tgctattgat aaatccacct gtttcaaaca aaactttagt cttaatggtt cctttggtga
    26581 ctgatttacg aattgaaccg tatttaccaa ttcccataga gtcgttacca ttatctgttt
    26641 tagcaactaa accacctata cgggctctgt tagcaacaat atcagcctcg acatatgctt
    26701 ttgcaagatt acctctccaa atctcaccgg caatgccagc catatattga cctttatcac
    26761 caacacctgt aagattaccg atgaaggcta catttgtcaa ctgaccacca ctatcgattt
    26821 tattgacgat acccgcaata ttgttattag caactacatt accagttacc ttaacatctt
    26881 caacagttgc atttttcacc atattggcaa caggtgctat tccgtcagcc caaggcatat
    26941 tgatatccac attacctaaa ttaatatttt tcacagaacc attttcaaca cggtcaaata
    27001 agggacgggc aatgttatga attgtatatt gtttaccatc aacacttgat agatgtccct
    27061 taaacgtacc tggaacatat tgtttgttag gtgttggtac attcgttgca ttcaaatctg
    27121 ctccgatttt aaatgtacca tttttatcag cattcatggc atctactaac tctttaaagt
    27181 tataataaac gtcatcaact tttggaactg gtttttcaat ataatgaaca tacttgttac
    27241 gaaccttcgt tgggtctgta tgttgaatca aatcttgagc ttcggcagtg attttataaa
    27301 gtttcttacc ttcttcagtc acttcttcaa ttttatcaac tgctaaacgt gttactttat
    27361 tatcacgact agtaattttt aagtacattt tcttcacatc ttcatctgaa ggtttttcag
    27421 tcataaaatc actaggtgtt tcagtaccat cgtcatttac cttcactaaa ttagtagatg
    27481 cgatattttt aatttcaact tttttcaaat ccaagcgaag tggtttctct tctaatgttg
    27541 cagtttcttc tcccttgcca cggtcatagg tcatagtggt ctctatctta tagtccttgt
    27601 agaattttaa atcttctacc tgcttatcta attgctctga agtcaaatct actgttttga
    27661 caatttcatt tccatcttta attgtcgcag taattttatt gattgtgact ccatctgtat
    27721 taactaaatg ataagaaaga gttgcggatc ggtttaatac atttgtattt gttctatcta
    27781 aggtcaaaac aggagccgtt ttttctttag gtttttcttt ggtacctttt acaagaattt
    27841 taggcttcat ttctttcaaa atagttactg tttcaactgg gtcactgatt tttttgccat
    27901 gtaactcttc atagctagta acaatttgac gttcgccatc ctcaccaact tgtctaacag
    27961 aatcatcgcc tataaatttg ctaggatcag attcttctat agtggtttta ggaatagtct
    28021 ctgtccgaat atcggtatgt aaagaaggca actcttctct ttctgatgca acttctgaaa
    28081 ctgtagatat tggttcagta tactctggaa tgtcgctaac aggtggctca atcaagtttc
    28141 cattttcatc cactcctgtt gtccctactg gctcagtata ttctgggatg tcgttaacag
    28201 gtggttcaat caagtttcca ttttcatcta ctcctgttgt ccctactggt tcagtgtatg
    28261 ctagtttctc atgaacttca ggctcaacaa ggtttgcacc tattggttgc gtatagtcag
    28321 gcttctcatg aacttccggt tcgcctttgt cggttacaac agcttcgggt aacactggtt
    28381 gaacttctgg ttcccccttt tcagtgacta ttgcttctgg taaggatggt tgaactgctg
    28441 attctacctt ttctgagact acggctactg gcaattctgc ttgaatctca gattcccctt
    28501 tgtcggttac aacagcttcg ggtaaagctg gttgtacttc cggttcgcct ttctctgtta
    28561 caactttggg ttgctcaact ggcgaaacag ttgatgctgg agaaaccgac gaaacagttt
    28621 tatcatcaag ctccaagtaa ccaacatatc tgtaaccaga aatttgcaac acaccatcac
    28681 gtccagactc atggatgtta gcattcaaat taagtgctga agcagttgat agtgtaacta
    28741 aacctgtcgc ccctacaatc aaaaatgtag ctattttttt atttctatta tcttttgtga
    28801 taatcacaat aagactcccg atagctaaca aacccaatgc agtcataata gattgagaac
    28861 ttcctgtgtt tggtagtgcg tctttttcat aatccaaagc atatgactct ttggattcat
    28921 caggtctacc ttgtttgagt tgatcacgtt cagtttgtgt caaactacta taatctaagt
    28981 aatgataagt cgatgcacca acaactgacg gtgcaaataa aagactcccc aagaataccg
    29041 aaccaacaat accctttatt ttccgaatgg aaaatttatc tttttttaat aatgacaatt
    29101 ctaatcctcc accttcactt tttatttcct aatagtaaac actatttttt attctcaaaa
    29161 attgaacttc taaaatcacc ggtttggtca agataagctt taaagattgc ttttttcagt
    29221 ttaagaactc tactatttgt ctcaggatat aatttggcaa cttcatcctc agcatcaaat
    29281 tttactgcat cttcaatcat tttttcaagt ctattaatat tatcaatagt cactttctgt
    29341 tgtcgagccc aagaaccgtt tggatcgata aaactaactt tatttaactt tccaaattta
    29401 tcttctcttt ctttatacat atctttcttg aaatctatcc aagaattata ctggttatta
    29461 aatacttttg aaagaacaat ttcatcagta actaaaccta tttgtttacc atatgatgta
    29521 ataacttttc cttgagcttt cgcttctttt tcatactgat tagaaatata tggaaccata
    29581 ccgtctttgt accccttggc tgccaataac tcaaatgcaa tacgtcgtcc cattaaatct
    29641 cctggtgttc ctattttact gctcaatgct gaataaattg gtgagaatag attaattgta
    29701 tagtagccat tccgcttata agttgcttca tctacataac ctcttcttgt tataatatca
    29761 tgttctatca aactgttaaa tgtagttaat ttagtaagtt cttctggttt taatctacgt
    29821 acaacattcg ttgcataaac agaatttcca tcaggatcag gtttatattc attttctatt
    29881 tttcttaata aatcatattt ttgatttgta tctaatttag ctacagcttt accttctagt
    29941 atttcaagca tgtatattac atcaaacata ttatgcatat acttatttag atcatcaaca
    30001 cttccaaatc gttgagttgg atctgctact tgaagccttg ttgattcttc tgattgatca
    30061 tattttaata tggaattaat agtgactgtt ggatcattag gatgatcagg agcttgtagc
    30121 agtcctttag cataaaactc tggtccaagt ccatttcgtc ttccatagcc tccaagataa
    30181 atttctctat ctgagttatg tgtcatttcg tgtgtataag ttaccgcacc atctttatca
    30241 agcatgcgat atgccatata atagaatgca tcccctgtag catatgctcc attagaatta
    30301 tgaacaacat tatttcccgc tggaccgaag aaatgtttta tagctggatg attagtttca
    30361 aaagttgctt gatatgtatc tttatcttct ttttcatctg taccaaatct aaatgcatca
    30421 tagactagaa cacttctata taatttttct ttatttttat catctagtat tctataccaa
    30481 taatcatagt gatctctaaa tctaacagcc gcttctttag cttttttctc aacatagtca
    30541 tttaaatcag ttcctgctgg atgttcttta cttctatatc tatcataagc accgaaacct
    30601 atagtcgaaa tgttggctat aataaacacc gatttttcag gtaatgttaa tagcggcaac
    30661 accatacttg gatatttcca agatgggcta gttaatctat catatactcc aattgaatat
    30721 ggactaccag ctattgattg cttatcactt acctctttaa tagtagattt ttcttctaca
    30781 atatatgctt tagtttgttc tttaaaccac tcattatttg ttttatctgg tacaaatgct
    30841 tttctatatt tctctaatgc atcaaataaa ttattagtct tataattttt cgctaataaa
    30901 acattataag ttataacatt attttttgct agtaagttat tatatcccga tttacctagt
    30961 tcgataacat tatctaacgc tgaactattt gatttaccga agaaatccaa gtggtacatg
    31021 actaaatctt tcgcttttgt atcacgatat ttgaagtcgt accaacgctc taagtatgtt
    31081 aatcctagaa gtaaagcttc tttattattt ttaattttct cgattacata attatctatt
    31141 accttattgt tattttcagc tattgcagca tctgcagtaa gaagttttgt caaactatct
    31201 ttcaaattag tttttgtggt attgaattgt tcttctaaat ataactccgt taaactaaca
    31261 tccttagaaa catctaatac ttttctaatt gcctctgatt tatattctac actatttaat
    31321 gctggtaata cttcatctaa aatattttga taattgcgaa gtaatgtatt cggtgtataa
    31381 attaagtttg taccgcctat tctatactct gctacatttg aaaaatcagt ttgataattt
    31441 acattaagtg tctctgatgt attatccgcg taatatagta atagtttatt tatacttgat
    31501 ttatttttgt taatatcact aattacttct ttatctttca tagggactac tgacaataat
    31561 tctttattat aaagattact acttgtctct actaaatttc cgtacttaac aattgtttct
    31621 ctattataaa acggtaatag tttttcaata tttttatata ctttttcttt tgatgaatca
    31681 gcattctcaa gatttttata gtcagttaca tatctagaag agaaattaaa atcttctgtt
    31741 ttaatagtat ttttagatac aaaagtagct tttttctctt ttatttgtgc atcttctaat
    31801 acttctcctc tttgaacagt agaatcctta gttaagattt catcatcttc ttgtactacg
    31861 ttcacaacct tattattttt attactgtat gtgccatttg cttttatacc attaaaatta
    31921 taacctgcaa tagcattacc attcgttaca ttaacatcgc ttagtacatt agaaagtcga
    31981 ccggattttt ctaatcctcc aaccggatcc caaagattac caactactcc accaacgttt
    32041 gcaaaacgtt gaacattatt tagatttcct tcagcataac tattactaat aagcgcacta
    32101 ttttctactg caccaactat tccaccaata gactggtctc cttgtgttgc atttgaagat
    32161 aaatcgattg atgcgaacga cttatctatt aatcccctcg gaccagaaag tttgcctact
    32221 aaacctccga tttgataact agcaactgtt ttatatgtat tagttattct acctgtatag
    32281 ctactgttag aaatagtaga attattaact tgtgatacta atcctcctat gctacgttcc
    32341 ccagctatcg ctccatcagc atgaacgtta tcaatatgag tattgttatt tgcttctttc
    32401 gcaagtgttg cagtatcgtc ctttgcggaa atattaacat cttttagtga aagtttttct
    32461 actgtagcac catttaattc cccaaataat ggttttttca aattataaat agcataatat
    32521 ttctcattat ttgttcctac taatattcca tggaatacat tattcacata actttcttga
    32581 ccatctggaa gttcaacttc acgagcatcc atagttgctc ctaaacggaa tgtgccatta
    32641 ggattgctat tcattgcatc tactaaattt ttaaatgatg tatatacccc agtttcactt
    32701 tgctgagttt ttggtaaata gtatgtataa ttttctagta cattattatt ttcatgttgt
    32761 atcagactat ttgcacttgc tactatctta taaacatctc tattatcttt agtatcatta
    32821 gtaatgcttg taactggtag catcacatct ttaaagttct ctgatttaac tttcataaag
    32881 taattttctg cattacttgg taaagaagca agtgaagtta catgtcgata cttattacca
    32941 tcttttgaat atagttctac agaagatata tttctaagtt ctaatttctt aataacttct
    33001 ttttcatctt tagcgtcatc ttttttctca tctttatgac gataaagttc atcgtatact
    33061 ggtggaattt cattacctgc tataatactc ttaatccaag ctaattgtgt aggagataaa
    33121 atcaatccgc ctgagcgatt ttgtgctcca ttttgatgca caccaataac ttcaccatta
    33181 gcattaaata ttcctccacc actcatacca ggatttccac cttgatattg aacaccgtag
    33241 gtgttattcg catattcttg ttccatttct accgtagcat cttttaagtg cgtatatttc
    33301 ccagctggat atccatgaac atttagtttg tcattaagat ttactttcga ataattttta
    33361 actaattcta catgttctct gccagttgtt tcaggtagct taatcactgc taaatcatag
    33421 ataaatcctt ttggataatc ttttttatta taaaaatgaa tattgttagc agtaaatgtt
    33481 cctttttgtc catcaggagt cacccactca tatacgtttt tagatttatc acctctaaat
    33541 tcgccagtag tattatcagt agaattatta atgaaattat gtgctactgt taaaagtaca
    33601 tttggtgcaa taaaagtagc tgatcctact ccagtaacct tcgacgaatc tgtaataaag
    33661 ttcatattaa ttaatgcact agattttttc tgaatactca tttcttctgg tgtattagta
    33721 ttttcattat ttggtttagt atcttctgca ctaggttgtt cgattttacc agtatattct
    33781 ttcagtgctt ctactttctc tggctcgact atcgctccgg cttgcacccc tgtgtattct
    33841 ggtagtggtg ctatctgctc tggttcaaca atagcgccag cctgcacccc tgtgtattct
    33901 tttgatggtt ctactttttc tggttctact attgctccgg cttgcactcc tgtgtattcc
    33961 ttcggtgctt ctaccttctc tggttcgact atcgctccag cctgcactcc tgtgtattcc
    34021 ttcggtgctt ctactttctc tggctcgact atcgctccgg cttgcacccc tgtgtattct
    34081 ggtagtggtg ctacctgctc tggttcaaca atagcgccag cttgtactcc agcatattcc
    34141 ggttcaactt tttcaggttc aacaatagct ccagcttgta ctcctgtata ttctggtagc
    34201 ggttctacct gctctggatc acccttgtca gtcatgactg cctctggtaa cgctggttga
    34261 acttctggta cgcctttatc acttactaca gcttctggca actctggttg gactgctgac
    34321 tcacctttgt cggttaatac agctgctggt agttcaggct gaattgcggg ttcaacaata
    34381 gctccagact gtacttcctt atgttctgca ccagtctcag cttgttcctt tataacttga
    34441 gcttttttag tacctttttc gactattctt gaaacaggcg cagtcgttga agttgaaata
    34501 atttctcgtg aaacttcttc attgtttaca gaaaatattc tgacgatttc aactttctta
    34561 cctaatgcac cttcttgttt tactcttaca gttccttctg ctaaatcagg attttcttga
    34621 atttcttctt gaaaatctat ttttgtttct atagtttcgt cacgatataa gagttccggt
    34681 ttgttcaact gacctgatag cacttcatcc tgtggattta atgtatttac cccagtcttt
    34741 tcctttggag aaatcttctc ctctttcttc gtttctagat tcttatgttc ggctaattgt
    34801 tcttgagaat ctgaagattg tttctcttct tttcttggat tgattaattc agtagagaaa
    34861 ggtttttcaa ctacttgaac ttctgtcggc ttagttgaag aaacaggtgt ttgttcctga
    34921 atagcttgta ctgttgatgg atggtctaca aaatttggtg taacattata atccaccttt
    34981 tgttgttttg taggagtggc tgctgatttt tcttgattac ttacttcaaa atcagaagtc
    35041 gtttttccct ctttgatata tccaatataa gtgtaacctg aaatctcttt aggaagaggt
    35101 aatttttctc cagaggtcaa ttcatagtcc gtattgtaat ttagcaaaag atgattttct
    35161 aaagcatgga ctgaaactaa gacaccattt cccattcccg caaccaatac taaatgtaat
    35221 accgttttat tcttaacctt tttcttggaa acagcaaaaa ttaaaattcc cagagcagct
    35281 aagctagcac cagcaactag ggcttgcctc tcattcttgc ttccagtatt tggtaattcc
    35341 gccagttgat tttgagaatt taacttataa acaagataat aagtttcatc atcattctcc
    35401 acgtatgtcg gaatatcata gacaagctgc ttcttttctt ctgatgatag ctctgaatct
    35461 gccacatatt tatagtgaac tcccgcagtt tcttgagcat ccacagatga actagctaat
    35521 acagacataa aaaataaact tgaaatcgtt gcagatacaa gtcctactga taattttcta
    35581 aatgaaaaac gctcttgttt ttcaccaaaa tacttttcca ttattcctcc ttgaaataaa
    35641 atttatatat gttacaaaga cctttattat attagtgtat tatctattat ctatagaaaa
    35701 ggcagtatac cttaattata ctcttaattt acaaaaaagt cttaaaattg agatgcgctt
    35761 tcatactttg ttttatatta tttgggggta caataacacc taccatgaaa tttacacggt
    35821 aggtgttact catatcacta atcgttctaa aaatggtttg aggcagttga ggagaattcc
    35881 ttctatccag cttccttgtg ctgatgagcg atggtcttcc tgcaggcttt tttttagaaa
    35941 atctcggact tgttctggtg cgatttcaaa ttcaaaggct ttcattttat agaaaaagtc
    36001 gatgagatga tcggacaggt attcagttga aaagggtact tcaccacttt ttcgatattc
    36061 taataagagc ctagaaaatc gagctttttc ttcaggaagc tcacgaaaat aggaattgag
    36121 gagccaagtc tgcttctgtt ttctttcaat tggatcctga ctggcaattc gttggtcttt
    36181 ttccagctct ttttggtatt gtttggcctt gatagcccgt tctgctctat ttttaccaaa
    36241 aagaattttt tcccacttgc gttcttcttg agtcagggtc tctgtaaagc caaagtaatc
    36301 ttgataagca cgctctgcgg gtcccatggc tagaaccaga ttgtctgcat attgcttggc
    36361 gattttatcc ctcttcttgc gttctttctc tgcctggata cggagttctt gttcgtagtc
    36421 aattttctcc ttgcctagct tgacaaggta gagttggtca tccgatttcc caagtaaaaa
    36481 gggtttgata cacttttcaa ggacttcttc catccgagcc tttttctttg gttccgcctt
    36541 ggtccaactt cctccctgaa agacttctag gaaaagctgg tagtctctct caggcgcaaa
    36601 ttgattgcca cgattgggtt tgaaaacacc tttttcccag agccatttta gaagtcgctc
    36661 gtcaaagtca cttttattga ccttgatttt ttcctttttc tgagcttttc tggttagatt
    36721 ttcaaccttt ctgagcagtt tttcttcctc ttccaattgc tggtcaaggg acaatcgatg
    36781 aaaatgacga acacagtcgc taccaattgg aaagaggcgt tggcctgtga caccgttaaa
    36841 gagttcataa gcgtatttga tggcatttcc acagacacaa ttgctacggc cgataccgtt
    36901 aaaaatcaag gaaacttcat tccattcctt ggtagcttgt tcccaagtat ccgctttcga
    36961 agcctgtaaa actgcatcgt gcagggattt tctaactgga agtgtcatga ggtctccttt
    37021 ctaatactca ataaaaatca aagagcaaac tagaaagcta gccgcaatca gctcaaaaca
    37081 ctgttttgag gttgtggata gaactgacga agtcagtaac catatataca gcaaggcgaa
    37141 gctgacgtgg tttgaagaga ttttcaaaga gtataagtta tacttttaca acttgaacct
    37201 cgtctttacc gagtaaaatc aagtattttt caatattttc aatcgaatag gctcgtgata
    37261 aagcctcttc gtatagagct aactgaccac gatagcggtc tacgagttga cttggttcat
    37321 catagcggtc tgtcttgtag tcgaacagaa caattttgtt ttcgtaaagc agatagccat
    37381 caaggatacc acggacaaca aagtcttcct gactcttttg gtctcgtttg agcatggaga
    37441 aaggttgctc gcgatagaga tggtcggtat tagcaagaat ttcctgaccg agtactgtgt
    37501 caaagaaagc aagaatttta tcaagattga tcttgtctct gacagcttgg ctagtttgaa
    37561 cttgtttgag tgtttctgtt aggctagcaa gggttagttg ctggctgagg tcaattctct
    37621 gcatgagttc gtgagtagca ctaccaatct cagctccagt taccttttct ttggttgaaa
    37681 aatctggcaa atcgaagctg attttcttgc ctactgactg accttgacca gcaatctcga
    37741 caccttccat atccataact ggttcgtaga atttcttgat ttgacttggg gtttgaacac
    37801 taggaagttc aatagctgcg cggtgaagag tattataaac ttccacctcc ttcagcattt
    37861 ccagagcttc tttgatggta tctgactgac gattgtctgc ttgggagcta tcttggagag
    37921 gactcttggt ttccaactct ccgatagctt ctctggtcaa ctgatcttcg ccaataaaac
    37981 gataactaaa gttgagcttg tccttagtaa acactttact gatagcccaa agccaatctt
    38041 ggaaattccg tgcttgcagt ctagtattgc tatttagttt cccatttttg gctgctgggt
    38101 attgcttgac ttccagcttt tcacgagaac ccttgccgac aagatagagc tttttctcag
    38161 cccgcgtcat agcaacatac agcaaacgca tctgctcaga atagcttgct agctgtaatt
    38221 cctcttcgtt ctgcctatag gtcagactag gaatggagag tttgatggtt ttaggatagt
    38281 ggtcttctac tgcccctgtc tccatcttgg caatatattt gacaccaaga ccattctgac
    38341 gactgagaat gacttctgac atagagtctt gcttgttgaa atcttgatcc atattgagga
    38401 taaagacgta aggaaactcc agccctttac tcttgtggat ggtcatgagc tctactgcat
    38461 cttttggcgg tgcgacggcc acgcttgcca aatcgtgctg ggcttctaag acttggtcaa
    38521 tcatacgaat aaaacgcgac aaacctttga aattgctctt ttcaaattga tcagcacgca
    38581 gtgctagggc atagagattg gcctgcctag caggaccatt cggcaaagcc ccaacatagt
    38641 cataataaaa acggtcgttg taaatcttcc aaatcaagtc atagagagag tgggttttgg
    38701 catacaagcg ccaagaagct aggatatcca tgaattgctt tagtttttca gctagagctg
    38761 tgtgaatcaa gcctttttga ctacttgcca ttttttgtgc attgaccagt ttctcataga
    38821 gattttcgtg gactttatcc tctgctttct gaagggacaa acgtgctagc tcatcctcat
    38881 caaaaccaaa cattggagac ttcataaggg caaccaaggc atagtcttgc aggggattgt
    38941 gaatgacacg aagagtgtct agcatgactt gcacttctag ggattggaga taattgtttt
    39001 gctctccgtc agttttgaca ggaatcccgt actcagacag ggcgaggaga atctggtcat
    39061 tacgactgcg gctggaggtc agaagggcaa tttccttaaa ggcaacacct ttttcttgat
    39121 gaagtttcag aatctccttg ataactaagc gcatttcgcc tgttagtttc gtttctgttt
    39181 gactctcttc ttcctcacct gtatcgtcct tgtcgtagag gagaaattct gccttgttgt
    39241 ctggattggg agtcagtttg gtattggcaa aaacaagctg gtgcttgtta tcatagttga
    39301 tttcgccgac ctcttggtcc atgagacgtt caaagacatc attggttgct gacagcactt
    39361 ctgaactact acggaaattt tccttgagga gaatgagctt gccttcttgg ggattttgcg
    39421 catagcgttg gaatttctca ttgaaaatct gcgggtctgc ctgacggaaa cgatagatgg
    39481 attgcttgat atctcccacc ataaagcgat tgtggccatt agacaacaat tccagcatcc
    39541 gttcttgaat atggttggta tcctgatact catcgaccat gacttcatgg aagcgctcct
    39601 gataagactc acgaacttgt gggaaatttt ctaaaatctc aatggtgtaa tggctgatat
    39661 cagcgaattc aaaggcattt tcctgtcgtt ttctctgacg ataagcctct acaaaatcgc
    39721 tcatgaaaga ttggaaggtt ttagctagtt tccaagtgtc tccatgataa cgttcttgat
    39781 agtcgagaat cgctatctgg tctgataatt gtcctagttt agtaaactgg gtctttctct
    39841 cttcgttgta ggcatcagcc aggggcttca aatcagcctt acggctggca ttagtcagag
    39901 cacgaccgtt tttctcctta gagatggcga caacacgcgc aagtactgcc tgataagcct
    39961 gactatcgaa ctcctgattt agggagccaa tttcatccag aattaactga acattttcta
    40021 aataggcagc ctttgcaaac tccttggcat cgttatccag atggtaacgg aaaaagcttt
    40081 ccaaatccca gagggcttgt ttgatttgct cagtcagttt ttctttttca ctggtaaaat
    40141 cagctttctc aaagcctttg aggaaagatt cactcagcca cttttgagga ttactggtgg
    40201 attggaggaa gtcatagatt ttatagactt gctgccgcag accccgttcg tccttgccac
    40261 gcccagcaaa gtttttcagc aaatgactaa agctctcttt ctgtttacct tggtaatgcg
    40321 cttcaaagac ctcatgaaag acttcgtttt cgagaagaag ttgctcgctt tggttttgta
    40381 aaatacggaa attaggtgca atatcaagca gataaccatg tttgccaagg aatttttgtg
    40441 tgaaagagtc catggttccg atggcagcgt tgggtaggtc tgccaactgg cgacccaagt
    40501 gttgtttgag atcgacatca tctgtttctt ggattttctt gctgattttt ttctctaaac
    40561 gttctttaag ttcagttgca gccttgacgg taaaggttga gataaagagt tgagaaattt
    40621 cgacaccacg cgccaattgg tccagaatgc gctctgccat gacaaaggtc tttccagaac
    40681 cagccgatgc tgagaccagg atattctgac cagaagtgta gatagcttcg atttgttcgg
    40741 cagttttctt ctgttccttg ctcgaatttg cttctgcttc ttgcagtttt tgaatctcct
    40801 cctcacttaa aaagggaata agcttcatcg atttaactcc tctcttattt tttcaaacca
    40861 agcttgcttg agtttttctc cgaccagacg cttgccatca gctaggtcca atttttctag
    40921 gaaacgggct tggcccagat ggtaattggc ttcaaagcct gtaatagcct gatgttgctg
    40981 gacgtatggg gcaatgcttc tgccattttc agtataagga ttgatggcga accggcctgc
    41041 taaaatcttc tcagcagctt tcttgtaaag ataggcattg tagtccagta ggagctgaaa
    41101 ttcctcatct gtcagttgat tagccttgtt tttgttataa aattcgccta aataactgct
    41161 ttctttttcc aagaagagcc cttggtattt catagatttg ctggcttcta ccactgctcc
    41221 tgccagactt tttaccgcca tcagagattg gacaggttca gccatttcca agtacatggc
    41281 gccgaaaaag ttctgcttcc cttctctttt tagggcagca agataggttg gtaactgaga
    41341 attgagccca ttaaagaaat gaggaaactg gaactgagtc agactggatt tgtagtctac
    41401 tactcctatc gctccattag ctttcaaacg gtcaatccgg tccaccttgc ctcgtacaaa
    41461 gacactgcgt ccattgtcta attgaataaa ggcttggtct tttccaccaa aatttgcttc
    41521 ttctttgatg gtttcgatgg ctggattgtg tcggagaata tgtccagtcg tccgtgcaac
    41581 atcaagcaaa acttccttgg taaactgggc ttccaaactt tcttgataaa tagcttcaaa
    41641 ttcgcgttct tgactggttt cttgaatagc ttgttctaga cgttggtcaa aggaatcttc
    41701 attaggcaac tgtaaggcgc gttcaaagat acgatgcaag aaattcccgt gactacgggc
    41761 atcaggacgc aaacgtaatt cctcctgcaa gcctaaaacg tagcgtagga aataactgta
    41821 ttcattgcga taaaactctg tcaaacccga cgtagacagg taaaactcct gtttggcagg
    41881 atagagagct tgcaaggtgt ccttggctaa ggtcttgctg cttggactgg ttgggatagc
    41941 tggattttcc agaccttgca gatctagttt tttacctatg acacgcgaca gaaccttgac
    42001 aaaagtcaaa tcttgctcag tatcgctcat ctcaccctgc tggtgatagg caaccagact
    42061 agacaaaaga ctgtgatagg accccatatc ctccttagac agtcctttgt gattcatcct
    42121 cttctctctc cgcctaaatc caaaatggat caactcttga agataggcag attccttact
    42181 ttcactttcg ttaaaaaggc ttggagccga caagaacaac tgcttacgag cagaattgac
    42241 caaggaaagc atagtgtagc gatttttctt gagattttca ctgctggcaa tcagtaattg
    42301 aacgccttct tcggtcgctt ggtttaggtt ttgcctttct tcatctgtca gaagactggt
    42361 gttttgagaa atttttggta aattgtcctg agttagtcca atagcataga caaagtcagc
    42421 agtcaatggt gcaatcaaat cgtaactctg caccagaaca gtgtccactg ttgctggaat
    42481 ggtacggtat tgggacaaac tcattccaga atggagcaag gctaggaagt cttccagact
    42541 aacctgtgaa ccagcaaaaa cagtcgcaaa ttgttctaaa acatggcaga aagccttcca
    42601 aacttcggct tgtctttcct gttctacagc ttccaaagtg gttgtcaaat cttgtaactg
    42661 cttggtcaca gctccttctt ttagaaagac actccatttt tgtaggaggt tttcagcctt
    42721 ttgttttcgg ctggcaaaga gggtttcaag aggtgctaaa attctcaggc ggaggacatt
    42781 caaacgctca agattaaatt ttccatggtg ggatttggtg aagatttgct gaaaggctgg
    42841 caagccattg ataccaagat agcggatata ttgctcaaaa gcatcaatat cagactgact
    42901 gaggtcagta tacaaatcag ttctaagaag attaatcaaa tcctcctgac gaaaacggta
    42961 acgttttaaa gctaaaatag actcgacaaa ctgagtcaag ggatgatgag ccatggcttc
    43021 gcttctacca agataaaaag gaatctgata ctggtcaaaa atggttttga gagataactg
    43081 gtaagaagct acatccccca agagaatacg aaaatgcttg tagctcaggt ctgagttctc
    43141 atgtaatttc tgacgaatac tacgggctac tagctccaac tcctcctttt gcgtcaaaca
    43201 agaccagatt tgtaaatttt cacggtcttt ctcatcgaca tccaaagcga gttctgaaaa
    43261 gtcataagaa gactccaaca aacgagaggc cttgtcaaaa ctatccatct tctcatgagt
    43321 ttgagaacag tcctgagcag gcgtttggta tttagaagcc agatgatgga gaaattttac
    43381 gctggcttgg tagagattgc cctcgctaaa aggactggta taggctttct tactagcata
    43441 agccccgata acaatctcaa cacctttgcc gtgaagtaag tccacaaccc gctcttcctc
    43501 agcagaaaaa cgagtaaagc cgtcaatgac caaggtgatt tgattaaaat cactacttac
    43561 cttgtcattc tcaatagcct caatcaaatg ggacaactga cttccctggg ctaactgacc
    43621 ttgattaaga taggctgtta ctttctcaaa aatcaagagt aaatccgccc tcttatcctc
    43681 atctgttaaa ttctccaagt ccaaaaaact catctgggat ttggtcatct catggtaaag
    43741 ctcaattaac tgctggatca attgaggatc ctgcttaata gcgccataaa cacgcaagtc
    43801 cttgggatcg agttcggcaa ggcatttgta aaaggccaac ccaagaccga tatcatcaag
    43861 agtagtttta gctggtaaat cattcaagac cagataacga gccatttgag caaagcgcgt
    43921 gacggtaatc gaaaaagaag cctgctggga caagcattcc agcacggcgc gttccttttc
    43981 aaaagaaaga gagttggggg caatgtagaa gacccgcttg ccagctgcaa ctagctcttc
    44041 tgcctctctt gttagaattt ctgtcaaaga agtccgaata tcagtataaa gtaatttcat
    44101 ctcagcctcg ttggaatttt tcatcaccct atattatacc atgattagcc tcgtaaatct
    44161 gttaaaatat ttaggccatc ctttcttttc ttcatcatct gctaaatctt aaatacttag
    44221 ctttacttgt attagataga ataagtctgg ctactgaaaa tcacataata aaaaagcctc
    44281 ggtaacaagg ctttgagttt tatgattgtt tcttaggtac ggaatacact tcaatgtgtt
    44341 gtcccagtat cttaatgtcg actggtagat tgtctgattt atcgccatca acatcggact
    44401 ctaattcgat atcagaagaa gttttaatat tacgtgcctt tatatattca atattcttga
    44461 tagaatgatt gaactatagt aaattgaaac tataatagta caccgtggat gctaaaatat
    44521 ttctagaaat taatttgatt tccctaatca agctgttcgt atcttatttc aatcta
//
LOCUS       DAVBDG010000009        43064 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBDG010000009 DAVBDG010000000
VERSION     DAVBDG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43064
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.009"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..800)
                     /gene="lytA"
                     /locus_tag="VQ526_000449"
     CDS             complement(<1..800)
                     /gene="lytA"
                     /locus_tag="VQ526_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2554215.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDT"
     gene            complement(1176..2546)
                     /locus_tag="VQ526_000450"
     CDS             complement(1176..2546)
                     /locus_tag="VQ526_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2554216.1"
                     /translation="MNKKRTVDLIHGSILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPRFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(2853..4019)
                     /gene="recA"
                     /locus_tag="VQ526_000451"
     CDS             complement(2853..4019)
                     /gene="recA"
                     /locus_tag="VQ526_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2554217.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(4074..5330)
                     /locus_tag="VQ526_000452"
     CDS             complement(4074..5330)
                     /locus_tag="VQ526_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2554218.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(5415..6431)
                     /locus_tag="VQ526_000453"
     CDS             complement(5415..6431)
                     /locus_tag="VQ526_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2554219.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(6439..6957)
                     /locus_tag="VQ526_000454"
     CDS             complement(6439..6957)
                     /locus_tag="VQ526_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2554220.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKITGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(6947..7390)
                     /gene="tsaE"
                     /locus_tag="VQ526_000455"
     CDS             complement(6947..7390)
                     /gene="tsaE"
                     /locus_tag="VQ526_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2554221.1"
                     /translation="MYIKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLSEELQYGV"
     gene            complement(7476..8096)
                     /locus_tag="VQ526_000456"
     CDS             complement(7476..8096)
                     /locus_tag="VQ526_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554222.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(8479..9342)
                     /locus_tag="VQ526_000457"
     CDS             complement(8479..9342)
                     /locus_tag="VQ526_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2554223.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            9846..11861
                     /locus_tag="VQ526_000458"
     CDS             9846..11861
                     /locus_tag="VQ526_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2554224.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLYVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            11863..12495
                     /locus_tag="VQ526_000459"
     CDS             11863..12495
                     /locus_tag="VQ526_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554225.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            12636..12914
                     /locus_tag="VQ526_000460"
     CDS             12636..12914
                     /locus_tag="VQ526_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2554226.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(13423..13836)
                     /gene="ndk"
                     /locus_tag="VQ526_000461"
     CDS             complement(13423..13836)
                     /gene="ndk"
                     /locus_tag="VQ526_000461"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2554227.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(13947..17624)
                     /gene="rpoC"
                     /locus_tag="VQ526_000462"
     CDS             complement(13947..17624)
                     /gene="rpoC"
                     /locus_tag="VQ526_000462"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2554228.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(17657..21268)
                     /gene="rpoB"
                     /locus_tag="VQ526_000463"
     CDS             complement(17657..21268)
                     /gene="rpoB"
                     /locus_tag="VQ526_000463"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2554229.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            21684..21754
                     /locus_tag="VQ526_000464"
     tRNA            21684..21754
                     /locus_tag="VQ526_000464"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21716..21718,aa:Cys,seq:gca)
     gene            complement(21952..23283)
                     /locus_tag="VQ526_000465"
     CDS             complement(21952..23283)
                     /locus_tag="VQ526_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2554230.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(23574..24398)
                     /gene="endA"
                     /locus_tag="VQ526_000466"
     CDS             complement(23574..24398)
                     /gene="endA"
                     /locus_tag="VQ526_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2554231.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(24437..24625)
                     /locus_tag="VQ526_000467"
     CDS             complement(24437..24625)
                     /locus_tag="VQ526_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2554232.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(24618..25901)
                     /gene="murA"
                     /locus_tag="VQ526_000468"
     CDS             complement(24618..25901)
                     /gene="murA"
                     /locus_tag="VQ526_000468"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2554233.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(25925..26155)
                     /locus_tag="VQ526_000469"
     CDS             complement(25925..26155)
                     /locus_tag="VQ526_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2554234.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(26213..27250)
                     /locus_tag="VQ526_000470"
     CDS             complement(26213..27250)
                     /locus_tag="VQ526_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2554235.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(27234..27722)
                     /gene="coaD"
                     /locus_tag="VQ526_000471"
     CDS             complement(27234..27722)
                     /gene="coaD"
                     /locus_tag="VQ526_000471"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2554236.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(27712..28251)
                     /gene="rsmD"
                     /locus_tag="VQ526_000472"
     CDS             complement(27712..28251)
                     /gene="rsmD"
                     /locus_tag="VQ526_000472"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2554237.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(28316..29308)
                     /locus_tag="VQ526_000473"
     CDS             complement(28316..29308)
                     /locus_tag="VQ526_000473"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2554238.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(29420..30103)
                     /locus_tag="VQ526_000474"
     CDS             complement(29420..30103)
                     /locus_tag="VQ526_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2554239.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(30159..30899)
                     /locus_tag="VQ526_000475"
     CDS             complement(30159..30899)
                     /locus_tag="VQ526_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2554240.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            30933..31211
                     /locus_tag="VQ526_000476"
     CDS             30933..31211
                     /locus_tag="VQ526_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2554241.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            31289..32215
                     /gene="yidC"
                     /locus_tag="VQ526_000477"
     CDS             31289..32215
                     /gene="yidC"
                     /locus_tag="VQ526_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2554242.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(32290..33084)
                     /gene="pflA"
                     /locus_tag="VQ526_000478"
     CDS             complement(32290..33084)
                     /gene="pflA"
                     /locus_tag="VQ526_000478"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2554243.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(33210..34460)
                     /locus_tag="VQ526_000479"
     CDS             complement(33210..34460)
                     /locus_tag="VQ526_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2554244.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(34527..35354)
                     /gene="purR"
                     /locus_tag="VQ526_000480"
     CDS             complement(34527..35354)
                     /gene="purR"
                     /locus_tag="VQ526_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2554245.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(35621..36562)
                     /locus_tag="VQ526_000481"
     CDS             complement(35621..36562)
                     /locus_tag="VQ526_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2554246.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(36552..37808)
                     /locus_tag="VQ526_000482"
     CDS             complement(36552..37808)
                     /locus_tag="VQ526_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2554247.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(37810..38472)
                     /locus_tag="VQ526_000483"
     CDS             complement(37810..38472)
                     /locus_tag="VQ526_000483"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2554248.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(38435..39091)
                     /locus_tag="VQ526_000484"
     CDS             complement(38435..39091)
                     /locus_tag="VQ526_000484"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2554249.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQSKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(39102..39980)
                     /gene="rsgA"
                     /locus_tag="VQ526_000485"
     CDS             complement(39102..39980)
                     /gene="rsgA"
                     /locus_tag="VQ526_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2554250.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(40573..41346)
                     /locus_tag="VQ526_000486"
     CDS             complement(40573..41346)
                     /locus_tag="VQ526_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2554251.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(42036..42908)
                     /gene="rsmA"
                     /locus_tag="VQ526_000487"
     CDS             complement(42036..42908)
                     /gene="rsmA"
                     /locus_tag="VQ526_000487"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2554252.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
BASE COUNT        12524 a         9669 c         8153 g        12718 t
ORIGIN      
        1 gtgtccttgt acttgaccca gcctgtcttc atggcacctt cttcgttgaa atagtaccac
       61 ttatcagcga ttttcttcca gcctgtagcc atttcgcctg agttgtcgaa ccagtaccag
      121 ttgccgtctg tgtgcttcct ccagcggtct gcaagcatat agcctgaact gtcaaagtag
      181 taccaagtgc cattgatttt ctcaaacttg tcttttggat aagagccgtc tgaatgtacg
      241 taccagtagc cagtgtcatt cttctgccag cctgtttcaa tcgtcaagcc gttctcaata
      301 tcatacttaa actgctcacg gctaatgccc cattttgcca agtaagggta tggatccaca
      361 tggtctgagt ggttgtttgg ttggttattc gtgcaatact cgtgcgtttt aattccagct
      421 aaactccctg tatcaagcgt tttcggcaaa cctgcttcat ctgctagatt gcgtaagagt
      481 tcgatataaa ggcggtaatc cgtcatgaac tcttctttag ttgaatggct ttcaatcagt
      541 tcaaccgctg cataggtctc agcattccaa ccgcccccaa cgtcccaggc accattatca
      601 acaggtccta cctgcatgat gcaaccgttc ccaacaatgt gcgagaaaaa acctaattct
      661 gggtctttcc gccagtgata atccgcttca ttctgtacgg ttgaatgcgg attcccagtt
      721 gagtgtgcgt gtacttgcct atatggttgc acgccgactt gaggcaaatc tgttcttaat
      781 ttactcacat taatttccat attctactcc ttatcaatta aaacaactca ttttttacaa
      841 tccagaacca gaaactcctt tatttctacc ttacaaagaa gacaatctta gtcacgatta
      901 ggcttgtaga tagaacctca aaacgcacta ttttgacact gtaaatagga ctgacaaggt
      961 ctgcattcta tctacaataa caccccagac taaaaagctt ttcaaaagta tatttttaca
     1021 gtctctatat gtccttttca taaatactat actttattat atcatataaa agaagtcaaa
     1081 agtctgttaa actattttca acaccaaact aaagaagaga acacaagttt tttcgatgtt
     1141 cactagagga aatggatttt attcagtaaa tccaactagg attgcacttt ggttgccaaa
     1201 attgcctttc cttcttttat caagggatga cggaatagtg agaagtacag ttgaactgtc
     1261 atggcaaccc agataagagg ttcacaaagg ataacaccct tatatcctgc ccaaggaata
     1321 atcaaaacca caaaaacgat ttttccgatt agttcaataa agctagaaac tagaggaagg
     1381 atcttctgcc ccaagccctg caagcaattg cgataaatca acaagaggct caaaatggga
     1441 taaaaggctg aactgatttg cagatagaga cttccatttt ctatcaagta accatctgtc
     1501 gaactagcca agaaggaaac caaggctgga ctggcaaaaa agaggaaaat acaaacaaaa
     1561 actgcccagg atatacttaa acgactgccg attcgaagac cttgaacaat gcggtcaggt
     1621 cgcttagctc ctagattctg agaagcaaag gtcgtcattg atgcagaaat agcggtcata
     1681 ggaagaaggg caaaggtcat aatgcgtcga gctgccgtct gggcactaat aatcactgca
     1741 ccaaatgtat taacagaaaa ctgtaaaatc acactgccga tagatacaat tgaactcatc
     1801 aagcccatag ccaaaccttg ctccaagaga tccgcgtaca agcttttgtc ccatttgaaa
     1861 tgtttaaacc gtggcaagag ttctggcaca cttttacgaa tataataaaa gcagagaacc
     1921 gctgataaac cttgcgaaat aatggtagca agtcctgcgg attgaactcc cagatgcaat
     1981 tgcgtaataa aatagagatc cagaaccaca ttaaccaagg cagagaaaat cagaaatccc
     2041 agggctgcta gactgtcacc aatagaccgc aacaagcctg caaaaagatt ataagcaaag
     2101 ctgacaccta cacaggtcac aatcatagaa atatattgat aagattgagg aagaatttct
     2161 gcaggagtat ctaagtattg caagagagga tacaagccaa gaaagcccag caacataact
     2221 agaatgctca aaagagcacc taaaatccag gtggctgcta ctgcttcctt gattttagtg
     2281 aaattccgag ccccataata acgagcaatg acaatcccca tgccattgcc aacaccaagt
     2341 gtaaaaccta caatcaggtc aaaaatcgct gtcgtcgctc ctactgcagc caaggattct
     2401 tgaccaagaa atcgtccaac aatcaagaca tcagcagtgt tatagagctg ttgaaaaata
     2461 tttgatagca agattggaaa ggtgaagctt aagagcgagg gaagaatcga accatgtatc
     2521 aggtccactg ttcgtttttt attcataaag ctattatatc agctttccag aggagcgaca
     2581 agaaacagca aacttatata tagcggaatg aaataaggac agtcaaatcg atttttaaca
     2641 atgttttaga aatagatgtg tactattcta gtttcgatct attatagttg gttgagtttg
     2701 gaatagtacg ctgtagctgc taaaacattt ctagaaatta atttgacttt cctaataaag
     2761 ttgttcatat cttatttcaa gctactatat ataatctaaa aacttgaatc aaaaatcgaa
     2821 aaagtagcgg atttttccac tgctttaaca gcttattctt caatttcgat ttcaagttca
     2881 tcgcctaagt caagcgtaac ttcttcattc actgcttctt ctttctttgg ctcatctttt
     2941 ttgttttcag tatcttgttc tgaaacttct tctccatcaa tcaagccaaa tttagaacgg
     3001 acttgcttat caatttcatc aaagatttct gggtgctctg ccaagtattt cttagcattc
     3061 tcagaacctt gcccaatttt ttcatctttg taagaatacc aagcccctgc ttttttgata
     3121 atatccaaat cgcttgcaat cttcaaaagc tcaccagtct tagaaattcc ttctccgtac
     3181 ataatttcaa ctacggcttc cttaaacggt ggagctacct tattttttac aaccttaatc
     3241 ttagtttctt taccgacatt ggtttctttt tggtcaccag ttcccttaat ttgtgtatta
     3301 ccacgaacat ccaagcggac tgaagcatag aatttcaaag cacgtccgcc cggtgttgtt
     3361 tctggatttc caaacatcac tccaactttt tcacgcaatt ggttgataaa aatggcaatt
     3421 gttttggttt tattgataga ggcgccaagt ttacgcatgg cctggctcat catacgagcc
     3481 tgcaaaccaa catggctatc tccgatatct ccatcaattt ccgcacgagg aacaagggca
     3541 gcaactgagt cgactacgac aagatcaact gcacctgagt caatcaattt tcccgcaatt
     3601 tcaagacctt gctctcctga gtctggttga gacaagagca attcgtcaat attgacacca
     3661 agggccgcag cataagctgg atcaagggca tgttccgcat cgataaaggc agcaatccca
     3721 ccttcttttt gcgcttgtgc aactgcatga agggcaaccg ttgtcttacc agatgactct
     3781 gggccataga tttcgatgat acgtccctta ggataaccac ctgagccaag ggcaatgtca
     3841 agagctaaag aacctgagct catcacttgc accttttgct ccgcacgttc acccaaacgc
     3901 atgattgatc ctttaccaaa gtctttctca atcaatttaa gagcgtcatt caaggccttt
     3961 tcacgttctg ccccaaattt ttttgaaatt tcttctaatt tttttggttt tttcgccatt
     4021 ctattctcct acattctaat gatcctcaga cctatctact attatatcaa aagttagtca
     4081 cttaataaag ccttgcgaac taggttaaag gcatgcataa ccgcaatatg acgtacatct
     4141 gctcggcttc tgcctccaat attaaccttg ataacctcag ttccttgctc ttgcgccaag
     4201 cctatgaaga ctgtcccaac tgggtgtcct tctaggctat ctggtcctgc cactccggtc
     4261 aaactaattc caaaatcaga ctgggtcttg cttcgtgcct gctcagccat cttctgagct
     4321 gtaaattcag acaccacacc atgttcttcc aaattcttgg caggaatatc caacatcctt
     4381 gatttttcct ccaagctata ggtcacaaaa ccacccttaa atatacttga aactccagaa
     4441 aaattcgcca cggtagcttg gaaaagacct gccgtcaaac tctctgcagc cgcgatggtt
     4501 ttcccttgcc ttttcagttc ttctaccaca atgctggcta aactagtttc ttccccataa
     4561 ccatagcaaa agtctcgtaa agaaattcct tcgaaagtct ggcagtccaa gatttgattt
     4621 tccaagatat ccagcgcttg attcgcctct tcttgactgc tagcctttgt tgacagacgt
     4681 agagtgactt ctcctgtctt ggcataaggg gccaaggtag gatcgatctg attatcaatt
     4741 aaatcagcca aaatcgtaac cagctggctc tcgccaatcc caaagaaacg aagaactcgg
     4801 gaatacagct tgctccctgt catcaacttg ggtagaagtt ggtttaagac catgggtttc
     4861 aattcacttg gcggacctgg aaggacgaca taggtcactc cgtctacttc taattttcct
     4921 ccaacagcca gtcctgtttc gtttggcagt ggaatcgctc cttctacaat ttgagcttgt
     4981 ctttcgttat tcggtgttcg ggcatagtct ggtcgcaggg caaaaaagat atccaacttc
     5041 tcctgagcct gaggatcaaa gactaatgct ttccctaaaa atttagctag ggtttgtttg
     5101 gttaggtcgt cctcagttgg ccccaaaccg cctgtcaaaa tcaccagact gctacgttga
     5161 ctggcaatct caagcaaaga caagagacga acttcattgt ctcctacagc cgtctgaaaa
     5221 tatacatcta ccccaatctc agctagtttt tccgacaaaa actgggcatt ggtgttgaca
     5281 atctgtcctg tcaaaatctc tgttccaaca gcaatgattt ctgctttcat gtttcctcct
     5341 acctatctat tcgtattttt ttgaaaaaat cgcaggaatt ttcctacgat tgattttttt
     5401 atttgtatca aaagttaatt atcttcatca ccaacaggtg ctctgccaaa taaatcttca
     5461 aataaaaccg cattggtttc aagctgagta acttcttctt gtcccaaaga acgtcggagt
     5521 atattttgca tttccaacat atgtgctctc gaaacaatct ggtaagaaac accttgaagt
     5581 atctctcctt caccctgcaa ctgctgagtt tcaatggttt taaatgaatc tttatagcct
     5641 agcaagttag ggatactttt tgcagacaaa tcaatattgg tctgcatatt gtcactcaaa
     5701 gcttttagaa tctcttgata atgaccaatg ctatttaaac tgagagcttt ttccatgact
     5761 ttttgaataa cttcacgttg acgtttttga cgaccataat ccccctcagg atcttggtaa
     5821 cgcattcgtg catagactag ggcttcttct cccccaatat gttgctcccc aacaccgata
     5881 gaaatggtat taaattcttc ttggtcactg atagaaattg ggaaacctag gatattattg
     5941 actgtaatac ctcctactgc atccactaat ttttgcaacc ctctcatatt gaccatcaca
     6001 tagcgatcaa tatggatatt catcattttt tgaatggttt ctatagcaag ctctgctcca
     6061 ccatctgcat atgctgagtt cagtttcgct tcatgagcct gaccattccc tgattcaatg
     6121 cgcgtcagaa tatcccgctc taaactcatc attgttgttt ttttcgtttt aggattcact
     6181 gtcatcaaga tcatgctatc acttctaccg acccaagttt cagttcgttc aacatttccg
     6241 gtgtccactc ccattaacag aatggttaga ggttcagtcg cttcaataac cttggtttct
     6301 tcgccgattt ttttataggt tttagctaag gtttctgtcc cttgttgata aatagtataa
     6361 gcaaaaacac ctactcctac tacagttaca gaaagtaaag ctagcaccat tccaataatt
     6421 tttttaacca tatttctact aacctatcag tttacccatc aagtaaacat cgataaattt
     6481 cccttcttct atatatgccc cacgctcttg gctaccttca atgacaaagc catgcttttg
     6541 ataaagatgg actgctgctt gattacgagt ttggacagtc agttggagac gacgcagaat
     6601 gccacttgct tgtgcccact ctatcgcttc ttctagcaac aaacttccca agccattatt
     6661 ccaatatctt tttccaatca caatgaagag atctccaata tgacggactc tcttacgctg
     6721 atcagctgta atatttacaa taccagtaat tttgccattt aagaatgcaa gtaaggttat
     6781 ctgattgtcc gaactagctt gcttgttgag gaatatttcc atctcctcac tagtcaagag
     6841 aataccatct ccgtctaggc tggtaaagtc tgtctccaaa ctcacacgat ttaaaaaggc
     6901 cactaattca gctgcatctt tgggctctgc ttccctaatg agcaattcat actccatatt
     6961 gaagctcctc tgacaatttc tcagcacgca aaccctttgc ctgaaaattt aaacggcgtc
     7021 catctgcttc ttttagaatt tccaattcta aataagcatc tggcaaggca tctcctaaga
     7081 gatttcccca ctcaataaca gtcacgccgc caccaaagat aaactcatcc aagtcgatag
     7141 aatcagcatc tccttcaata cgataaacat ctaggtgata aagtggaagt cgaccttcat
     7201 actctctcac gatagtatag gtaggacttt taatcatttg agaaatctgt aatccttttg
     7261 caagtccttt agtaaaggtc gttttacctg cacccagttc tccaattaag attaaaacat
     7321 cattctttgc taatagatgg cccaaacgct cccctaaggc ttgcaactct tcttcatttt
     7381 ttatgtacat actcttatta taccaaaaac ttttcttttg tgtctatttt cctactaaac
     7441 ttatcatcat aacatccata aaaaacaggc tttctctaaa agaaaatgag cgtaacaatg
     7501 accaatacaa gatctcggaa aatatgacca taaaaggaaa cttccttctt aaccgaattt
     7561 gggacaagat aggctgcaaa aaacaagccc agtccaatat aaatcagaag tgagacaatg
     7621 gtcattggat ttcttaagaa aagaagtgtt gctaaaatag tcaccaacac tgtctttttt
     7681 ctgtccagca tagcaagaaa atcgcgcacg tattttttca agggtaaaaa aatcagcaaa
     7741 tctagcccaa ataggaaaaa gaaggatggc aataaaaagt caactaattc ttgctgcagc
     7801 gtatttttga tgaacaagtt atctgacaaa acaagaacag ctcctaacaa attaattaag
     7861 agtaacatac tgtaaaaaag cttcaccgac ttcttactgg ctaggacact atggacttct
     7921 tgcttacgag tataaagata atttactcca gcacagattc ctgaaacgaa aaccatgctt
     7981 ccgatgaaaa aagctgtact ttgtttaaag gacaagatgc attccttcca taggaaacag
     8041 ctactcaaac tgatttgaat taaagctaac aaaaataaga ttctcattga tttcatcttc
     8101 tctctccctt cctaccaatc attatactag gagaaaagag agaactgttt ctaatcttct
     8161 caaatgtctc tttaagacgc taaacaaaca ctagagacta atactcaatg aaaatcaaag
     8221 agcaaactag gtagctagcc acaggttgct caaaacactg ttttgagatt gcagatagag
     8281 ctgacgtggt ttgaagagat ttacgaagaa tataaatttg aaatcatgaa aatccgtcaa
     8341 acgggtggtt gttttgtctc gcacctcacg gagcgagacg gactcagagt cacataatta
     8401 taaggctgat agtattaatc taactatcag ccttacaggt tatttaacgt ttcagaaaaa
     8461 ctataatgtc aagattaact aaacagtatc tagttccttc aaataatttt ctatcttcat
     8521 caacattaaa ggattgttat aaatcttaca taactctctt gcttctatat aataattttt
     8581 gacttgttct ctgtctagaa atttggctcc agcatttcct acaagaataa gtagaggagc
     8641 caattggtag cttgtctgtc tttgtttaca gagttcaatc gtttcaagag cttcttggat
     8701 ggcttcatta tatttttcct ttgatactag gtagtgagcg tagttgtaac gaactctgat
     8761 gtagccaaat aaaaactctt gatggtccaa attttttgtc tgatacaact ctattaaatg
     8821 agagtagttt gcctcatatt cttgttcacg acccactaag gaatagaaat tagatagagt
     8881 attcaacgcc tttaaataaa tcagagtatt tgaagagact tttaataata tattttccaa
     8941 tgaagaaatt gcctcacact tactgtcata ttgatagaag tcaataatag ctttaatcca
     9001 ttcaaggtaa gttcggtctt ctagtgttag aaaagtactt cgttcaatct ctattctata
     9061 aatatattcc aaatcgtcat aatttctatc gtctaatagt cgagcagaca attgcttgaa
     9121 attagagagg ttagatttaa tttcaatttg ttcattaaaa aaataatcta atggtacttc
     9181 aagtcgttga gagagtttga acaaaaggtc tgcggaggga atgaaatgcc ctctctcaat
     9241 tttactaatc tgactttgtt cacaaattcc ttctgcaaga gtttgttggg agagtctcaa
     9301 ctcttttctt ttcaatctga atttctctgc gagtgtattc attaaagata ataaaccttc
     9361 ctatttgctt aattgcatta taaaattttt agttcaaaat agcaagttaa aagaataaat
     9421 aattctttat ggtaataaac taaaaaaagt caaaaattac ataaaaatgc ttgaccgaat
     9481 aatatatata tattaaaata aaaacaagga aaaagaaaag ggtttcacga atgaagaaaa
     9541 atcgtggaat tcaaaaatta gctatattag tattactagg tgtttttatg cttagtaaca
     9601 caattcctta ccaacagttt attcagaaga atagacaatt ggagattcga gtccaatcac
     9661 aaaagaagtc caatggtctt gatgttggga aggctgattg atttcgtatt actttaaaat
     9721 tcaactattt agagaaaggt attctgatga gagcttgtaa caataccaga tagatattag
     9781 taagcttgtt agtttataga gggcaggggt atttctgccc tctgttttag aataaggaat
     9841 gcactatgaa aaaattattt atactcatat ccaacctgct tgctagtttg ttctttgttt
     9901 gggtatttac catttggact gatacatatg tctcctatta ttaccccaac gttgttgtac
     9961 atgattcttc ccctgaaaca acttttcagc atgttgctac acgcttggaa aaactagcag
    10021 aagaaacaga tagctttatt gcgattcaac accaagattc taactcagaa ggtactacag
    10081 ttttttctta tacgaccttt ggggacggaa agttgcctga tgggcttcag gaaaaaaaac
    10141 tagaagatgc tcaaagtagt agtgttgaaa caaactattt tgtattcgat ggacacctag
    10201 atattcactt gctaagggag gagctaagtc aacttggctt gactaacatg aacctgacaa
    10261 ttccatctaa actatctacg ttaatggcta tctttagtaa cggatttcag ttaatcagtc
    10321 tattgatttt catcttaacc tttgtagcac tgactttaat tagtcaaatt agccaattac
    10381 ggtcgtcggg cattcgcctc atttcaggag agaaacggtg gtccattttc cttagaccag
    10441 ttggtgagga tttgaaaggg atagctgttg gtttcagttt agctggcgtg ctcgccattc
    10501 ttatgcaaaa aattctgtcg cttccaacgc aatccctaat gaccatagga gcaggcttgc
    10561 tcagttataa tctcatcttg ttgtcgatct ccctgttttt cgctcaactc ttcgcagttg
    10621 ggataaaaaa gatacacctg atgcagatta taaaagggca agtgcctgtc agaggaatca
    10681 ttagtttgat tttaatcggt caactattag cgattattat tgtaacgctg ggaataggga
    10741 gtagcttaaa gtattcccaa gcctggcaac agcatcggat tggacaagag gcttggagtc
    10801 aggaaaggca actgattacc ctatcaatca gccgtgaagg aacgagtcct ggttttgatg
    10861 aacaagctca aaggaaactc agaacttggt atcaattgat ggatctggct gtttcagaac
    10921 aaaaggcttt cttatctaga caccagttaa ttgaccgtac tttgcaaaat ggcatggctt
    10981 cctccaaaaa cttgataacc tctacagaat ggcacgatta caacccgaat ggcaatgtcc
    11041 ttatcgtcac accgcaatac ttggagcgtc aaaacattcc tgtagataca actattgagc
    11101 aaaagatgaa tcacctttat gtaggggagt ttgtcttatt gctgcctgaa cacctccgtt
    11161 cagaggaaga acattataaa tctgtttttg aagacgactt aaccagtcgc atgtctagtc
    11221 aagatgaacg acagcaaatg actgctacgg taggttattt agaatcaggt caggatcgtt
    11281 ttgtgtataa tacgacccct atttcttacc agcagttttt gaaagatcca atcatcattg
    11341 ttataacacc ccaatcaact ggtccacagt ccattttgtt ttggatagac gcagtacaga
    11401 actacgttct ctttaatcaa ttgtctgatg cccaggagct tatccagaga caaggcattg
    11461 aaaattgggt ctcagaaatg caaacaggtt accacaacta catcacatta ttggataata
    11521 ttcagaggga acgttgggta atgctagcag gagctgggct tgggattgca acttcaatct
    11581 tgttgtttaa cactatgaat aggctctact ttgaagaatt tagacgtgcc atttttatca
    11641 aacgcattgc aggtctcagg ttcttagaaa tccatcgcac ttatctcttt gctcaactgg
    11701 gtgtgttttt actgggattt gttgcgagtg tatttcttca ggtagagata ggagttgctt
    11761 tcttagtctt gttactcttt actggtctat ctcttttaca gttacatgtc caaatgcaga
    11821 aagaaaacaa gatgtccatg cttgttttga agggaggtta atatgattga acttaaacag
    11881 gtgagtaaat cttttggaga acgagagtta ttttcgaatc tttcaatgac atttgaggct
    11941 ggaaaagtct atgccttaat tggttcaagt ggtagcggaa aaacaacctt gatgaacatg
    12001 attgggaaat tagaacctta tgatgggacg attttttacc gaggtaaaga cttggccaat
    12061 tataaatcga gtgatttttt ccgtcacgaa ttgggctacc tcttccagaa ctttggctta
    12121 attgaaaacc aaagtattga agaaaacctt aagctaggtc tcattggtca aaagttgagt
    12181 cggtcggaac agcggttgag gcagaagcag gctttagaac aggtcggcct gacttatctt
    12241 gacctagata agcgcatctt tgagttatcg ggcggagaat cgcaacgggt tgccttggca
    12301 aaagttatct taaagaatcc accctttatt ctggcagatg agccaacagc ttcaatagac
    12361 ccagcaactt ctcagttgat tatggagatt ttgctatctc ttcgagatga taataggcta
    12421 atcattatcg caacacataa tccagcaatt tgggagatgg ctgatgaagt gttcacgatg
    12481 gatcgtctga aataaaaatc cttgttttta attgcacgat gagttactga aatattatca
    12541 tgaatcaata attggagtta atttagaatt gtactttatt aatattgagg taactttttc
    12601 ttgataaagg aagaaatagt ggagaggaag ttagaatgaa aaaattcgac aattatattg
    12661 ttgagaagcc ttgcgattct aattcagata aactgcaaaa aatcttaata attgaaaatt
    12721 tggtagatga tattttgcaa tttcctctca gaatcaataa tagtgtagga gagattttcc
    12781 tcctacaacc gtttcaaaag aaaactatct ttattccatg ttattttgag gaagatattg
    12841 tgaaagtcaa agatgatgat aaagttgagt ggaatttgtt agaatttcaa aaatttagag
    12901 catttttggc ttagtaatct gtgttgaagg ctcaaaacct atggtaaaaa agtcgctttg
    12961 aaaacgtatt gcctccaaag atttagttaa ataatgattt aacacaaaaa gaaattattg
    13021 aagttctgga aagatgttgt ttcagtattg agaaaaggtg ggaaaaactt gcgattttca
    13081 cagagaaagg aagaaaaagt atagaaatat agtcaattga aacaagaaca ggacaaaaga
    13141 accttttgtg ccatattttt ctcctttcgc tttacaattg gcttgaacac ctttattgta
    13201 tcgcgtttgg agtttttttg gtataacctt cgacgcgcac ccgcatagcg ggtgtttttt
    13261 tgtctcgcac ctaacggagc gagacgaact aatagtcact taatcaaaaa acgcaccata
    13321 tcaaaaacta aaaagtttga tatcatgcgt catgtcttaa actaattgac tatactttct
    13381 attcaaatga gcttttaacc aattgattga gccaatccac tcttaaaacc aaagagcaat
    13441 ttctcgctta gctgactctt ctgaatctga accatgtaca acattttgga taacctcatt
    13501 ttctccagca gcttttgcaa aatcacctcg aatagtgcct ggtaaagctt cttctggacg
    13561 agttgcaccc atcatggtcc gccaagtttc gattactttg ggaccagaaa tgacacccac
    13621 aagaactgga cctgaagtca tgaattcacg aatcggtggg taaaaactct gaccaaccaa
    13681 gtcctgatag tgctggtcaa tcaactcttc tgaaacttgt gaacgaaact ccaatttttc
    13741 gattgtaaat ccacgttgtt cgatgcgctt taacacttca cccactagcc ctctttttac
    13801 accatctggt ttgatgataa agaatgtttg ttccataccc gtctcctttg tcagcttctt
    13861 tcttttattt taccacattt cgtggaaaaa tggagaaagt tttcagaata gagaatgaga
    13921 gaacccgagg gttctctcat tctctattat tctactgttt cttccacagt ttcaacggca
    13981 gtatccacaa ctacttctgt tgtttcttca tttccttctt cctctactgg aggattaagg
    14041 tattcttctt cgttgacagc atatggttca aggttacggt aacgggccat accagtacca
    14101 gctgggatga tcttaccgat gataacattt tctttaagtc caaggagatg gtctttctta
    14161 ccacggatag ctgcgtcagt aaggacacga gttgtttcct ggaaggaagc cgctgacaag
    14221 aaactgtttg tttcaagtga ggctttggta attcccataa ggactgggcg acctgtcgct
    14281 ggaactccac ctgcgataag gacatctttg ttggcatctg taaagtcatt gatatccatg
    14341 agggtaccca tgagaagatc tgtatcacct ggatccatga cacggacttt acggatcatt
    14401 tgacgaacca ttacctcgat gtgtttgtca ccgatttcta ccccttggct acggtaaact
    14461 ttttgtactt caccgagaag gtacgtttca actgacaaga catcacgaac tgcaaggaga
    14521 cgttttggtt ggatagaacc ttctgtcaga gcagcaccac gcgctacttg gcccccaact
    14581 tcgacacgca tacgagctgt aaatggaacg acatattcac cttcgccagt ttcaccctta
    14641 acaaagactt tcttggtacg agttgatgca tcttcttcga tagcagtaac ttgtccttta
    14701 acctctgtga taaccgcttc ccctttagga ttgcgggctt caaagatttc ttggacacga
    14761 ggaagaccct gagtgatatc ggtatttgag gcaaccccac ctgtgtggaa ggtacgcatt
    14821 gtaagctgtg taccaggttc cccgatagat tgggcagcaa tggtaccaac tgcttcacca
    14881 acttcaaccg catcaccagt cgccaagttg ataccgtaac agtgacggca gacaccgtga
    14941 cgagtgttac atgtaaatac agaacggata gtcacttctt ccacaccagc attgacaatt
    15001 tcacgcgcct tgtcttctgt aatcaattca tttggaccaa taatcactgc accagtttct
    15061 ggatgtttaa cagttttctt agtgtaacga ccgttgagac gctcttcgag agactcgatc
    15121 atctcttttc cttctgcgat agaacggatc aagagaccac ggtcagttcc acagtcgtcc
    15181 tcacggatga taacgtcttg ggcaacgtcg accaaacgac gagtcaagta acctgagtcg
    15241 gctgtcttaa gggccgtatc ggtcatacct ttacgagcac cgtgagttga gaagaacatt
    15301 tccaataccg acaaaccttc gcggaagttt gaaaggattg gcaattccat gatacgtcca
    15361 ttcggagcag ccatcagacc acgcataccg gcaagctgtg agaagtttga gatgttacca
    15421 cgggctccag agtccatcat cataacgatt gggttcttag gatcttggtt agcaatcaag
    15481 cgtttctcaa gtttttcacg ggcagcacgc cattcagctg taacagcatt gtaacgctcg
    15541 tcgtctgtga tcataccacg acggaattgt ttggtgattt gttcgacacg tttgtgtgat
    15601 tcttcaatga tttcagtctt gtcatcaacg actgggatat cggcaatacc cactgtcaat
    15661 cctgcaagag ttgagtggtg gtaaccgagg ttcttcatgc ggtcaagtag ggcagaagtt
    15721 tctgtcgtac ggaaacgttt gaagatttca gcgatgatat ttccaaggtt tttcttcttg
    15781 aatggagggt tgagctcaag attgctgata gcttccttga tatctccacc aagtggcaag
    15841 aagtatttag ctggaacacc ttctgtcaag ttggcattgt ttggttcttg caagtatggt
    15901 agcccctctg gcatgatatc gttgaagaga attttaccaa ctgttgtaag caagacctta
    15961 tgtctttgct cttctgtcca aggcttgttg aggctgtctg ttgcgatacc aacacgtgag
    16021 tggaggtgaa cataaccatt gcggtaagcc ataaccgctt cgtcacggtc tttgaagacc
    16081 attccttcac cttcgcgacc agcttcttcc atggtcaagt agtagttacc caaaaccatg
    16141 tcctgagatg gagtaactac cggtttccca tctttcgggt tcaagatgtg ctcagcagct
    16201 agcatgagga tacgagcttc tgcttgtgct tcttctgaaa gtggtacgtg gatggccatt
    16261 tggtccccgt caaagtcggc attgtaggct tcacagacaa gtgggtgcaa gcgaagagcc
    16321 ttaccatcaa ttaagactgg ctcgaaggct tggataccca aacggtgaag ggtcggtgcg
    16381 cggttcaaaa gcactgggtg ttctttaatc acttcttcaa ggatatccca gatacgctca
    16441 tctccgcgtt ccaccaagcg tttagctgct ttgacgtttt gcacgatatc acgggcaacg
    16501 atttcacgca tgacaaatgg tttaaagagt tcaatcgcca tttcacgtgg cacaccacat
    16561 tggtacatct taagagttgg accaacggcg ataacggaac gtcctgagaa gtcaacacgt
    16621 ttaccgagca agttttgacg gaagcgtcct tgtttacctt taagcatgtg gctcaatgat
    16681 ttcaatggac ggctacctgg tcctgtgatt ggacgaccac gacgaccatt gtcaatcaaa
    16741 gcgtcaactg cttcttgaag catacgtttc tcattttgaa cgatgatacc tggtgcattt
    16801 aactcaagca aacgagccaa acggttgtta cggttgataa cacggcggta aaggtcattc
    16861 aagtcagatg aggcaaaacg gccaccatcc aactgcaaca ttggacgaag atctggtggg
    16921 ataaccggaa ggatgttaag aatcatccat tcaggtttgt ttccagactt gtaaaaggca
    16981 tccaaaacat ccaaacgacg gatggctttg acacgttttt gtccagtagc tgttttcaat
    17041 tcttctttga gttcagcaat ttctttttca agatctactt gcttcaaaag gtcttggatg
    17101 gcttccgcac ccatcttggc aacaaatgaa ccataaccat attcgcgcaa gcgctctcgg
    17161 tattcgcgct ctgtcatgat agacttgtgc tcaagtggtg tatccttagg atcaatcacc
    17221 acataagccg caaagtagat aacttcctcg agggcacgag ggctcatatc aagggtcaag
    17281 cccatacggc ttggaatccc cttgaagtac cagatgtgag atacaggagc tttcaattcg
    17341 atatgtccca tacgctcacg acgaactttc gtacgcgtta cttcaacccc acagcggtca
    17401 caaacaattc ctctgtaacg aatgcgtttg tacttaccac aagcacattc ccagtctttt
    17461 gtaggaccaa agatcacttc atcaaagagt ccttcacgtt ctggtttcaa ggtacgataa
    17521 ttgattgttt caggtttttt gacttctcca taagaccatg aacggacttt acttggagaa
    17581 gctagggtga tttgcatact tttaaaacga tttacatcaa ccactatttc ttccctttct
    17641 attctaagtg aactgcttat tcttgttcag cagcttcttc tgttgcttcc gcttttgttg
    17701 ctttctcagc ttcttcagct tcaaaggctg ctttagcctc ttgggctgct ttttcgcggg
    17761 ctttttcaag gtcatctacg tggatgacat cttcgtccat tccttcatcc aagtcgcgaa
    17821 gttccacttc ttggtcatct tcgtctagga cacgcatgtc aagaccaaga gattgcaatt
    17881 ctttgacaag aactcggaag gattctggaa cacctggttt tggaattggt ttgccttttg
    17941 taatagcttc ataggctttc aaacgtccgt tgatatcgtc cgacttgtaa gtcaagattt
    18001 cttgaaggac atttgacgca ccgtaggctt caagagccca aacctccatc tcaccgaaac
    18061 gttgtccacc aaactgagcc ttacctccga gtggttgttg ggtaacagtt gagtatggtc
    18121 cgactgaacg cgcgtgcaat ttatcatcaa ccatgtggtg gagtttgatc atgtacatga
    18181 ctccgacaga aacacggtta tcaaacggtt caccagtacg tccatcgtaa aggatcgttt
    18241 tggcatcgct atccatacct gcttctttaa cagttgacca aagatcttca gaacttgctc
    18301 catcaaagac tggtgtcgcg atgtgaatac caagagtacg agctgccata ccaaggtgaa
    18361 gctccataac ctgaccgata ttcatacgtg atggtacccc aagtgggttc aacatgatgt
    18421 cgactggagt tccgtctgga aggtaaggca tgtcttctac aggaacgata cgagagacaa
    18481 cccctttgtt tccgtgacgt ccggccattt tatctccgac cttaatctta cgtttttgag
    18541 cgatgtaaac acgaaccaac atgttaacac ctgattgcaa ctcatctcca tttacacgtg
    18601 taaagatctt aacatcacga acgacaccat cggcaccgtg tggtacacga agagaagtat
    18661 cacgcacttc acgagacttg tctccaaaga tagcgtgcaa gagacgttct tcagctgaaa
    18721 gatctttctc acccttaggt gttactttac ctacaagaat atcaccttct ttaacctcag
    18781 caccaatacg gataatcccc atttcgtcaa ggtctttgag ggcatcttca ccaacgtttg
    18841 gaatttcgcg agtgatttct tcaggcccaa gctttgtatc gcgcgtttct gattcgtatt
    18901 cttcaaggtg aacagatgtg tagacatcgt ccttcaccaa gcgttcgctc atgataacgg
    18961 catcctcgaa gttgtaacct tcccaagtca tgtaggcaac gattgggttt tgtccaagcg
    19021 ccatttctcc attttccata gaaggtccgt cagcgatgaa atcgcctttt tcaacgacat
    19081 caccaacttt tacgagagtg cgttggttgt aagcagtacc tgagtttgaa cgacggaatt
    19141 tttggatgtg gtaaacatcc aatgaaccat cttcacgacg aacttctacc ttgtcagcat
    19201 ctgcgtaagt aactttacca tcatactgag caatcacagc cgcaccagaa tcgtgggctg
    19261 cttggtattc cataccagta ccaacgtaag gtgcctgagg attaatcaat ggcacagcct
    19321 gacgttgcat attggctccc atgagggcac ggttggagtc atcgttttcc aagaaaggaa
    19381 tacatgctgt cgcaacggca actacctgtt ttggtgaaac gtccatgtag tcaacaatat
    19441 tagctggata ctcttggttg accccttggt gacgtcccat gacaatcttc tcagcaaagg
    19501 ttccatcttc attcagacga gagttagcct gagctacagt atattcatct tcttcatcag
    19561 ctgtcaacca aacaatttcg ttcgtgacaa cacctgtttc acggtcaacc ttacggtatg
    19621 gtgtttgaac aaaaccatat ttgttcaagt gtccgtaaga tgacaagtta ttgatcaaac
    19681 cgatgttagg tccttcaggt gtctcgattg gacacatacg accatagtga gtgtagtgca
    19741 cgtcacgtac ttcatatccg gcacggtcac gagtcaaacc accaggtcct aaggctgaca
    19801 aacggcgttt gtgagacaac tcagaaagcg ggttgtgttg gtccatgaac tgtgacaact
    19861 gtgatgaacc aaagaattct ttaactgcag ctgttacagg acggatattg ataatttgtt
    19921 gtggtgtcaa gacttcattg tcctgaacag acatacgttc acggacatta cgttccatac
    19981 gagaaagtcc caaacgtact tggttggcaa gcaattcacc aaccgcacgg atacgacgat
    20041 ttccaaggtg gtcgatatca tctacacggc caagtccttc agccaagttg aggaagtagc
    20101 tcatctcagc aaggatatct gcaggagtca ccgtacgaac cttgtcatct gggttagcat
    20161 taccaatgat cgttacgacg cgatctggat cagttggagc aacaaccttg aatttttgaa
    20221 gaacaacagg ctcagtcaca acggctgcat cgtttgggat gtagacaatc ttgttcaagt
    20281 cgccatccaa atggctttca atgctttcaa tcacgctacg agtcataatc gtaccagctt
    20341 ctaccaagat ttctccagtt tcagggtcta ccaatggctc tgcaatggtt tggttgagca
    20401 aacgtgtttt aacattgagt tttttattga ttttgtaacg accaactgct gccaagtcat
    20461 agcgacgtgg gtcaaagaag cgagccacaa gcaagctacg tgagctttca gccgtcttag
    20521 gctcacctgg acgaaggcgt tcgtaaattt ctttcaaggc ttcgtctgta cgagagtcca
    20581 ttggattctt gtggatatct ttttcaacag tgttgcgaac caattcgctg tcaccaaaaa
    20641 tatcaaagat ttcatcatca cctgagaaac caagagcacg aaccaaggtt gtaaatggaa
    20701 tcttacgagt acggtcgata cgagtgtagg tgatatcttt tgagtcgctt tcaagttcca
    20761 accaagctcc acggttaggg ataacagttg aaccatagcc caccttacca tttttgtcta
    20821 ctttgtcgtt aaagtaaaca cctggtgagc ggaccaactg agaaacgata atacgttcac
    20881 caccattgat gatgaaagta cccatttctg tcatgattgg gaaatcacca aagaaaactt
    20941 cttgggtctt gatttcgcct gtttctttat tgatcaagcg gaaggttaca aaaattggtg
    21001 ctgagtagct agcatcgtga atacgagctt cttctagcgt gtattttggt tccttgattt
    21061 catatccaac aaattccaac tccattgtgt ctgtgaagtt tgaaattggc aatacatctt
    21121 caaacacttc cttaagaccg tggtctagga aagctttgaa tgagtcagtt tgaatttcaa
    21181 tcaaatttgg taagtcaaga acttctttga ttcttgaaaa actacgacgg gtacgatgtt
    21241 tcccgtattg aacgtcatgt cctgccaaga tgattctcct ttgtaaataa gttccaagcc
    21301 ttgtcaatca ggcttttcta atcgccatat ggttgtaaac cccttatcac cgtgtcctct
    21361 tgacgaattt tcagaatctt taagcctctg ttacaaatgc tcaaaatctt gaaaaaaagc
    21421 acaaaaagag cagctaaatc tgactttttc agaagattta actgctgtga gccttgtctg
    21481 gacaatattt cagacaaaac ctacgacaaa tgattaccca tattataccc tatttagcta
    21541 gatttttcaa ggggattcag taggtttttg gtaatttttt tcccatagaa aacttggcat
    21601 cacattcgaa tcacgctatg gtacaaaaaa ctgaaaaaac tattgactga aaatcatttt
    21661 caaggtataa taataaacgt taaggcggta tagccaagtg gtaaggcacg gctctgcaaa
    21721 agcttgatcg tcggttcaaa tccgtctacc gccttctata acttgattta tcaggtttca
    21781 aatgaacaga aagcccaatt tgaagggctt tttttatttt ccctcgaata aatacgtata
    21841 actttaaaaa cttttggagc gagtttgtgg cagagttctt tccatggcat aattcccttt
    21901 tgaaatcaat tgaaaaaagc ttcgtcacgc ctggtgacaa aagcctctag tttattctgt
    21961 ttcttcttct atttcgactt ctgtgatttg gacttttacc ttggtaacac gtccattctt
    22021 cactttatca ttggttaaga taaactgttt gttttggctg accaattcat agctgagttt
    22081 ctcagtcgtt ggaatggttc ccactcctgt caaataataa ccagcgatgg tatcaacgtc
    22141 atcactttcc agttcaacat caaagtaatt attgaaatca ttgagggtca tagttccttg
    22201 aacaatgtag gtatcctcgc caatctgatg aacatctatt gctgctttgt ccgtttcatc
    22261 atcaatttca ccaacaattt cctctaagag gtcttccaaa gtcaccaaac cagccatacc
    22321 accgtattca tccagcaaga ttgccatttg tctttgggta tttcgcaatt cttttagcaa
    22381 gtcatccaca aaaatagttt caggtacaaa aagtggatct tgtaaaattc tcttccaaac
    22441 aatattgtca aaaccgtcca caaagcctgc cttaaggaga ctcttggtgt gaatgattcc
    22501 aattacattg tccttatccc catcataaac cgggatacga gaataatttt gttttaaaat
    22561 actttggata atggcttgac tatcatcctg aatatccacc ataaaggcat ccgttcgagg
    22621 aaccataacc tctcgtgcca tcagttcatc gagcgaaaag acaccttgta gcatctcaat
    22681 ctcatcagca tccaatgttt cttcactatt tgtcagcata taggcaattt catcacgggt
    22741 catcttttca tccgcatcat cgaatgacat aggagtcaaa tggctcaaga aattggtcga
    22801 agcagctaaa agccaaacaa aaggactgac tagttttccg atcccaatga taatcggcgc
    22861 tgtacgaatt gccaaggcat cctttagatt aagagcgatt ctcttaggat ataattcccc
    22921 aaaaacgatg gaaatatagg tcaaaaatgc caaggataga aaagttgcca cggcttgtgc
    22981 tgtttcgcca ttcccaagcc aagaggcaat cacacgtcct agagtatcag ttaaactcgc
    23041 ccctgataag attgtaatca gggtgattcc tacctgaatg gttgataaaa agtggttagg
    23101 attttctagt accttcagca ggcggatgta gcgtctgtct ccttcttccg ccttttgttc
    23161 aactcgggca cgattaagag aaaccatggc catttctgtg gctgagaaaa aggcatttaa
    23221 taccgtcaag ataaacaata aaacaaattg tagcaacaaa ttctgactac ttgggtcttc
    23281 catagttctt ctcctaaaat agtatcttat cctttattat atcacaaaat ttaatccaat
    23341 acatattatt tttttcttag cgtaacttcc atctctctcc caaaactgga agttagtcta
    23401 agacggattt ccactgattt cacgcgtttt tctcatacta aaagctaggt tgtgggaaac
    23461 tggaagttag ttttagaagt tactattttt tcttagtacc tgttctagcc ctgcttaaac
    23521 tagtaaaaag aactcctaca tccataagtg acataggagt ttaggcttat cttttactga
    23581 gttacagtta cttctccagt tcggtaatcc agttgaagtc ccttttgaac attgggaact
    23641 agaacattga attccaattc tccatccgaa gacttggctt caatctgtga agctgaggga
    23701 actaaatcct cgtttgaagc gtagtaaagg gttacacggt aacggacacg cttgttttgg
    23761 tccaaggctt tacgcacctt gctttcatag tagttttgac cagtcgaata ctcggcttgt
    23821 gcctgatttg cccaggctgt ctgaacagca atgtttttag gattgcttgt tgaggcatca
    23881 aaaccatcca aaccaccgat taaggcatag cctaacaaat gacctctatc gactgcatgg
    23941 gtataagagc cctttagatt cttgacctga tgccaacctg gaggagtcca agaagttgaa
    24001 ccattcccag tttctttacg attcttatac tgacgagtgg ccttagacaa gagggcatta
    24061 gctacggttg gaacagtttc cttacccact gtctttgttt tattgtcagc gtagggctta
    24121 cttgaaacct tggcatctag atttgtttta ttaccattga cgataaaagc acctgagcca
    24181 ttccactcca gactcccctt tatttgactc ttgactgcgt ctgttaagac actctctgcc
    24241 aatgcttgac taggagcttc aggcgcttgt tttttctgac taagcttggt tttaggacta
    24301 ttagctgtcg actgcatctg cttgatataa tagctccctg tagacaaaag caataacact
    24361 agcagtccga ttagtgtctg tcttgttttt ttgttcatat ttctccttat ctttagaaaa
    24421 aggctggttc tccagcctaa tttcctgtaa atttatgaat caattcctgc cattttgctg
    24481 gagacaggat agcccatgaa tcttgaccct tgcccaagat tccataacct accattaaac
    24541 cgattcctag agccagagta cctaaaatca gtactatgat gactaaaagt aaacgcttga
    24601 ctacgtagct tgatttctta ttcatcttca tcacttgcct caatccgctg aatcttagca
    24661 cctagctgcg ccaacttctc atggaaaccg tagtaacctc tatccaagtg aaccaattta
    24721 ccgaccacag tttctccctg tgctaccaaa cctgtcaaaa tcaaagctgc actggcacga
    24781 aggtcagttg aaagaacttc tgctccctgc aaaggctgtc caccaacaat acgagctgta
    24841 tcacggataa tctcagaatg caagcccatg cggcgcatct cttctaggtg ttggaaacga
    24901 ttttcaaaaa ctgtctccac catggttgat tcgccttttg caactgtcat caaggctgta
    24961 aattgagcct gcatatctgt tggaaatcct gggtggggca aggttttcac atgaacagct
    25021 tttagatttt ccagttgaga acgaacacga attccttcgt cttcttcaat tacttcaaca
    25081 cccatttcaa gtaacttggc aatcaaggga cggttgtgct cccagacagc gtctcgaatc
    25141 aagacatcac caccagtcat ggcagcagct accataaagg ttcctgcttc gatacggtct
    25201 tggactacat tgtgagtcgt accatgaagt ttctcaacac cagtaatggt tatagtctct
    25261 gtaccagcac ctttgacctt ggctcccatt tcattaagga gaatggctaa gtcaacaatc
    25321 tcaggctcac gcgcagcatt ctcaatcact gtcaccccat cagccagagt cgctgccatc
    25381 atcaagttct gcgttgcacc aacacttgga aagtccatat agatatgagc gccatgcaag
    25441 cgttctgcct tggcttcgat gtaaccagct gtctgactaa tcttaacccc catagcttcc
    25501 agacctttca aatgaagatc aataggacgg ctaccaatcg tacaaccacc tggcatggat
    25561 accttggcat gacccacacg ggcaaggatt ggccctaata caacgatgga ggcgcgcatc
    25621 ttgctgacat acttgtaagg ggcttcctca gtgatgtcgc cagtagcatc caccttgaca
    25681 agatgagctt cctcatcaaa gtcaaccttg gcattcaaac caccaactac ctgattcata
    25741 ataaagacat ccgacaaaat cggaacattc tgcaagacgg tctttccttc acttgctaga
    25801 atagtcgctg ccaacaaggg taagactgca ttttttgctc cctcgatcgt cacgcttcct
    25861 accagacgat tatcgccacc ttgaaccaca attttatcca tactcgtttc ctttactctt
    25921 gattttataa taatcctcta agcgcttctt gccacaaatg atacaggcta ataaagaaag
    25981 aactcaggat gtagcctatg acaatgctaa aaaaggctac taataaacga acttttcttg
    26041 tattatcatc tgtcactttt aaaaccttct cccatctaac aagatccttt aaaaggtaaa
    26101 aactcacata aataaagagc atatgactac ttagagtgaa taataattga accatttgac
    26161 tattatacca tcttctctgt ttgtgcgcta gtttggaaac ttcactcaaa aattagggat
    26221 tgtttttcaa gtaatcaatc gcatcttgta gggttttaac gggcacgatt ttcatatccg
    26281 tcttgattgt tttagccgct tctagggctg tttggtagtt gtttttcgcg tccagatgcg
    26341 ccttttgttc ttcttcgcta acagggttat caggagcaaa gaaaatagca gcacctgccc
    26401 tagccgaagc tacaaccttc ttatcaatac ctccaatgtc tcccacatta ccatcgcggt
    26461 caatggtacc tgtaccggca acaatacgac cattacgaag atctgggtga gctatttgag
    26521 tatagatagc tagactaaac atgagaccag cacttgggcc gccaatacca gctgttgaaa
    26581 agctaattgg gacattgctg attacctctg tacggtcaat caagccgatt ccaattccat
    26641 ttttgccatt ttccaaggtg ataatctttc cttctgcaga cttggtttgc ccatcctctt
    26701 cataggtgac cttgacggaa tcccctaatt tttgagaact gacgtaatca atcaagtctt
    26761 tggaactatc aaaggtctga tcattgactg ctgtgactgt atcagagata ttgagaatcc
    26821 ctttaaaggt tgaattatcc gtcacattca aaacataaac tccaaagtac ttgagttcga
    26881 tatccttacc agctgttttt agtccttgat acttggccat attttgcgat gtttgcatgt
    26941 agaattgatt gattcgcata aattcaacat cggaagaacc acctgtagtc tcctgagcac
    27001 tacgaatatc tgtaaaaggt gtcaaccaag cataaatcat atgagctaaa gtggcatgtt
    27061 gaacaccaac cgtaacgaat tgataggcac cagcttcctt atcttctgtg tcatttactt
    27121 taaggacttg gcgaatatct tccgaaccac ctggaacctc tatataataa ggcaagggca
    27181 ctacaaatgc caagaaagtc acaatcaagg ccgcaatgac gtataagggc catctaatct
    27241 tttttttcat ttcttatttc ctccaaaata ctctcgggaa catggcaggc aatatcctga
    27301 ccaaacttca aaagctctcg aacacctgat gaactgatat agagatgttc aggccggcta
    27361 tgtaaataaa tagtctctat atcagaagac agctgatgat tgtagtaatc aaaactagct
    27421 tcatattgca aatccgccgc attcctcaac ccccgtacta ggaaagtagc ccccagtctt
    27481 tttgcaacat cgaccaccaa ttcatcatga gaagccacga cttcaacatt ttccaaatgt
    27541 cccaaagcct tttctagccc ccgtttacga ttttcaagag ggagaaatcc ttgtttgtgg
    27601 ggattaaaaa aaatacccac ataaagctta tcaaaaagtc tgctcgcccg ttcaatgata
    27661 tccagatgcc catttgtcat cggatcaaat gagcctgtga ataagccaat cttatctgac
    27721 atagactgtc accttactaa ttccataaat cttttccttc cagataccca gacaggcaat
    27781 ctcttctgga agttcaacgg ccttatccgt ctcgcacaca accataacat cttcagaaaa
    27841 aagctctctc ttagccattt tttcaatatc tgctacgatt tgttccttgg catagggagg
    27901 gtctaagaaa acgaggtcaa attccccaga tacctgttcc aatgccctct ctgcatccat
    27961 cttgaggagt tgaaattttc caacttcctt ggtcatctgg atattttcag ccacgatggt
    28021 ctgagcctta cggtctcgct ccaccaaaac agcactggac atgccacgcg atactgcttc
    28081 gatagataaa ccaccactac ctgcataaag gtccaagact cgtccccctt caaagtaggg
    28141 accaatcatg ttaaaaatgg ctcccctaac cttatccgaa gtaggtcttg ttgtcttgcc
    28201 ttctagtgtc ttgaggggac gtcccccata gattcctgat acgattttca tactgtttat
    28261 tataccaaat tatagacaaa aagagaaaga aaaccgaacc ttgcggttcg attctctaca
    28321 aaatattttc gtaagtatcg cggacttctt gaggccaaac acttgtttgc acttctccga
    28381 tgtgtctctt gcgaagtagg aacatggcca tacgagattg tccaattcct ccaccgattg
    28441 tcaatgggaa taggccattc aacaaagact tgtgccattc caattccaag cggtcttcat
    28501 cacctgtaat ttccacctga cgtctaagag tttcttcatc tacacgaatt cccatagaag
    28561 acaactcaaa ggctccacct aaagactcat tccagacaag aatatcacca tttagaccct
    28621 tgtagccatt ctcagactcg cttgtccagt catcatagtc tggtgcacgt ccatcgtgcg
    28681 gtttaccatc tggcaactcg ccaccgatac caatcaaaaa gacggctcca aattctttac
    28741 aaatcgcatt ttcacgttct ttaggtgtca agtctgggta gcgttctacc aattcttctg
    28801 tatggataaa ggtgatttgt tttggcaaga tagactcgat gtcatagcgg gcttcaacag
    28861 ctagctcagt caggcgaata gccttataaa tcttctcaac tgtttctttt agataaacga
    28921 tgttacgctt accatttggg ataaccttct cccagtccca ctggtcaaca taaacagagt
    28981 gggttgcatc caaggaatcc tcatctggac gaagggcttt catgtggaca aagagaccct
    29041 ctccttcacc aaagccaaaa cgagccaagg tgtggcgttt ccatttagca agtgagtgca
    29101 ccacttcata agtagcatca gggatttgga gaaccttgac cgatactgga ttttccacac
    29161 cagacaggtt gtcctgcatt ccgtcaccga ccttactcaa gataggacct tgaacttcga
    29221 caacttctag cttatctttc aaatactggg taaaagtgtt tttgacaaag gaaatttctt
    29281 cttgttgatg gataaaactt ttcttcatag acaactcctc aaaaatttaa taaacgtatt
    29341 atacacctat ttccaagaaa agaaaagaga aaatttaaaa aaaatcagtg tcactcaaaa
    29401 cactgatttc atagaccttt tagtcattgc gaccaaagat acgtagaata cttaggaaaa
    29461 gattgataaa atcaagatag atgctgagag ccattgacac cacccagcct gtcgctacac
    29521 gaccttgtga ttgttcatag gcgaggcgaa tcttttggtt atcccaagca atcaaccctg
    29581 aaaaaaccaa aaccatggct acgctaatca tataatcaaa gaagccacta gccaagaaaa
    29641 tattgactac catagcaatc agtaaaccaa tgagagctgc catcattgct cgaccaatcc
    29701 cactcaagtc tttcttggta aaaataccaa ctgccgccat gacaaagaag agaagggcgc
    29761 tcgatacaaa ggcagataaa actgtacccg gagtatagaa agccaccaca aaactaaggg
    29821 taaagccgtt taaaacggag taaagtaaaa atactggaag agcagctgga ctatttcttg
    29881 aagccatgct actggcaacg aaaaccaaag ccaactccgc aaaagtagca atggtcaacc
    29941 agagacgccc ctgcatcaaa aagtaaacca actgagactg aaagaccgtc aacataaggc
    30001 ctgataccaa agcagatagt ccgattccca gaccaacaaa ggcataaacc ttagcgtaaa
    30061 attgattgag acctgcacgg tcatgtataa tagtgtgatt catccatttc tccttttctg
    30121 tgaacatctt tccttgatta taacaaaaaa agttaaaatt agcttaaatg gaagattaaa
    30181 ataccggctg caaccgcaac attcaaactc tcggcctgcc ccttcatgct aatatggacc
    30241 aactggtctg cactttcagc cataagggga ctaattcctt gaccctcatt tcccataact
    30301 agtacaaaat tttctataga aggcagttct ctgtaatcaa cagaatcttt agatagggtt
    30361 gttgctagca ctgggatagc taccttttta gtttcgtcaa gaagcgcttg actagtcatc
    30421 cggtaaatgg gcagatggaa atgactacct tgcatggaac gtagggtttt gagactgtag
    30481 atgtctgccg acttatctga aacaatcact cctgtaaaac ctgctgcatc cgcagttcga
    30541 atgatagttc ctacattacc aggatcttgc acatcttcca aaaacaagaa cttgccctga
    30601 ctcaaatcag cttgtcctac ttcttctttt tgaaccacgg caacaattcc ctgtggagtc
    30661 tgagaatccg ccaaatctag caaaatatcc tctgacaccc agacagtttg cgaaaaagta
    30721 gctaactgat ctcgataatt ttctagggca aagattttct caatcgtcac tccagcttgg
    30781 acagcttctt caaacaagtg ccagccttca atcaagtagg cagacttgcg gtattttttt
    30841 tggcgtaatt tcttggcatt ttttaccaca gaattggctt ttgaggttat aatagtcata
    30901 gaaatattat aacacaatca aaggggtttg gtatgcaaaa ggttagaatg attgcccaag
    30961 gtagggtaca gggagtcggc tttcgttggg gtgtttacag cttggcactt gaaattggtg
    31021 gcatcacagg tcgagtatgg aataacgacg atggcacagt ggaaatctta gcccaagcag
    31081 actcatctgc tatcatggca aaatttatcc aagaaatccg aaaaggaccg acaccttttt
    31141 caaaagtaag ctacttagat gtcaaactaa gcaactttcc tccctactct gactttaaaa
    31201 tcgcaaatta ggtctccaga actattgtat attttgtaaa aaaacagtag aatagaaagg
    31261 tataattttt aaagaaggaa taaaaacagt gaaatctatt aaacgttttg cactctcagc
    31321 tatgggagcg gctatgttgc ttgtcttgac tggttgtgtc aatgtcgata aaaccacagg
    31381 tcagccaaca ggatttattt ggaataccat cggagcccct atggctgaag ctatcaagta
    31441 cttcgctact gataaaggtc taggctttgg tgtcgctatc atcatcgtaa ccattatcgt
    31501 gcgcttgatt atcttgccac ttggtatcta ccaatcatgg aaggcaacgc ttcactctga
    31561 aaagatgaac gccctcaagc acgtccttga gccacaccaa acgcgtctca aagaagcgac
    31621 tactcaagaa gaaaaactcg aagcccaaca agctctcttt gctgctcaaa aagagcacgg
    31681 tatcagcatg tttggcggtg taggatgttt ccctatcctc cttcaaatgc ctttcttctc
    31741 tgctatctac tttgctgccc aacatactga aggggttgct caagcaagct acctaggcat
    31801 tcctctaggt tctccaagta tgattttggt tgcctgtgct ggtgtccttt actatcttca
    31861 atcgctcctt tcacttcacg gagtagaaga tgaaatgcaa agagaacaaa tcaagaaaat
    31921 gatttacatg agcccactca tgatcgtcgt cttctccctc ttctcaccag ctagtgtcac
    31981 actttactgg gttgtcggtg gtttcatgat gattctccaa cagtttatcg tcaactatat
    32041 cgttcgtcca aaacttcgca aaaaagtccg tgaagaacta gccaagaacc caccaaaagc
    32101 aagtgctttc tctaaaccaa gtggacgaaa agacgttacc cctgaacaac caactgctat
    32161 cacaagcaag aaaaaacaca aaaatcgcaa cgctggaaaa caacgttcga gataagaaga
    32221 aaaaggctta gctgatttgc taagtctttt tgcaaatcaa aagcccgatg tttccatcag
    32281 gcttcttttc tatccatgta cacgtttcat ataatcttga taactttcgg tatccatgag
    32341 ttttttagcg ttcttgacgc gatctgctgt tggtggtttg actccttcga gggaatatgg
    32401 aattccaagt tcacgccact tgaactcacc catggtgtga taaggtagaa tttcaaactt
    32461 atcaacattt ttgagggtct tgacgaactt accaagttca atcaagtcat catctctgtc
    32521 tgtcaatcct ggaactagca cgtggcgaat ccagacaggt tttccaatat ctgatagata
    32581 ctgggcacaa gccaagatat ttttattggt ttgtctagtg acaatcttgt gctgttcttc
    32641 gttgatttcc ttgatatcca aaagaaccaa gtcagtgaca gccatgagtt tgtcaaactt
    32701 ctcaaggtaa cgtggtttat tacggaaagg aagagcacag gtatccaagg tacagtggat
    32761 tccttgttcc ttagccttgg tgaagagagc aatcaggaaa tcaatctgca agagagcttc
    32821 tcctccactg actgtaatcc cacccttatt tccccagaaa ccacggtagc gcaaggcctc
    32881 tgtcaagaca tcatctaccg tccgttcacg tgacttattg gactccatag cccaagtgtc
    32941 tgggttgtgg caatactggc aacgcatgtg acagccctgc aaaaagacaa taaagcgaat
    33001 accaggccca tctactgacc caaagctttc tgtcgaatgc accattcctg tcacttgtcc
    33061 ataatcaatt gtttcttcag acatatcgtt accttccttg aaaacgtttt atagttttat
    33121 tatatcacga cttgaaggaa aaacaagatt tttgcctatt ttttagaaag caatctgttt
    33181 ttcaatttca ttttcaatct attatcagat tattcttcaa aatcgaagcc atataaggtt
    33241 gcaaaatagt cctcagggcg ctctgcacgg cggatttgac gggctttacc ttcttcggta
    33301 tagaggattt ccgcagaacg taatttggca ttatactggt agcccattga aaatccgtgg
    33361 gcacctgtat catgaattac cagcaaatca ccgatttctg tatgaggcag ttcgcgattg
    33421 actgcaaatt tatcattgtt ttcacagagt gaaccaacca catctaccgc ttcagctggt
    33481 ccatctggat gggtcacgtt gctaatatga tggtaagctc cgtacatagc tggacgcatg
    33541 aggttgactg ctgaggcatc cacacctaga taggtacggt aggtttcctt cttatgagtc
    33601 actcttgtga ctagagcacc gtgaggtgcc agcataaaac gacccaattc ggtgaaaatc
    33661 ttgacctgac caagacctgc tgacgtaaga acttcttcat acaccttacg aactccctca
    33721 ccaatcaagg cgatatcgtt cggctcctgg tctggacgat aattaacacc aataccgcca
    33781 gaaagattga taaagtctag ccaaatgccc aacttttcct tgatttcaac agccagttca
    33841 aagagctgac gagccaactc tggataatag agatgggtca cggtattgga cgctaggaag
    33901 gagtgaatcc caaaagtctt ggctcctttt tccttcaaga tagcaaaggc ttcaaagagc
    33961 tggtccttgg tcatgccaaa cttagcctcc ccaggattgt ccataatgtc tgtccccagt
    34021 tcaaaaacgc ctccaggatt ataacgacaa gagatgattt ctggaatgcc tgctactctc
    34081 tccagatgtt caatatcttc aaaggcatcc aagttaatgg tcgcacccaa ttcacgtgca
    34141 taggcgtatt ccttgtctgg cgtgttgttg gaagagaaca taatctcaga acccagaaag
    34201 tccagtttat ggctcatcaa aagctctaca taactagagc agtccacacc acaaccttct
    34261 tcttggagaa ttttcaaaat agctggagtt ggagtagcct taactgcaaa atattcctta
    34321 aagcccttgt tccacgaaaa agcttggttg acggctcttg ccttctcacg aatccccttc
    34381 tcatcataca agtgaaaggg agtcgggaac tcggcaacaa tcgcttctaa ctcttctcta
    34441 ttgataaatg gtgttttcat aagcttcctt ctattctgtt tgcaaagaaa ggctgggaca
    34501 atcgttccaa cctttagttc tatctcttat ttatcttcgt caaagatatt gccaacctca
    34561 acatcgatgg cttggttctt gacatcaata ttagttacct tcaagagtga cttgtagtca
    34621 aactgctttt cacgttcttc ttgggcattg tccgcaaaga ccgctacacc tgccagttct
    34681 gagtcgaact cgcgcaagag actaatcata ccgttgaccg ttccgccacc tttcaagaag
    34741 tcatccacaa tcaagacacg gctacctgcc ttaagactac gttttgaaag gaacattttc
    34801 tcgatacggt caccacttga acctgaaaca tagttgacgc taacagttga accttcggta
    34861 attttcaggt cacggcgcac aatgacaaaa gagacattga ggacattggc aactgcattt
    34921 gcaagtggca cacccttagt tgctacggtc ataaccgcgt caattttttg gtccataaag
    34981 cttttggcaa taatacgacc aatatttttc aagatggctg gtgtgctaag caaatcagac
    35041 agatagatat aaccacctgg caagatacgg tcactttctg acaacttggt acgcaagtct
    35101 tcaaccattt ccttagcatc ctgactcgaa atagacggtg tgaagatgac acctccgcca
    35161 gccccagtca ctgtctggat atgaccgatt tcaatttcct caaaggcgcg tttgataatg
    35221 acgatatctt ctgagatgga tgatttagca gactcatact tttcagcaaa agtattgaga
    35281 ctagttagtt tataaggatt attaatcaaa tagttggaaa tgacaaccat ccgatcactt
    35341 cttcttaatt tcatttctat ttcctattcc atttttattc aaaaaatcaa aaagcaaact
    35401 agaaagctgg tcgctggtgg ttcaaaacac tgttttgaga ttgtcaatag aactgacaaa
    35461 ccctgtaata tacctgcata tatacatacg acaaggcgat actaccctag tttgaagaga
    35521 ttttcgaaga gtattcattt ttgtctttta cttattatac catattcaca taaaaaaacg
    35581 aacattctta tcctaaaaaa tgctcatttt tcttaaatta ttaatctaaa tctggtttat
    35641 agaaggaacg attatccata gcgaagattt tattggtcat ctctccttta tccaccaaag
    35701 caagagctgt tgacatcatc atcatgcttg catccagatt gtcaatcata tggataatct
    35761 ctgcttccat aatgcgtgga cggactgggc ttccatactc aagcaagccg tggtgactga
    35821 ggatgacatg acgaagcaaa acgacttctt ccttggtatc atcgatgccg agttccataa
    35881 ctgtcttggt aatttcgcta tcaatgagag cgatatgtcc aagaagatta cctcgcactg
    35941 tgtactctgt ctggtctggc cccgtcaact cgatgacctt agctaagtca tgcaacataa
    36001 tccccgcata gagcaggctc ttattgagct gaggataaac ttcgctaata gcgtctgcca
    36061 aacgcaccat ggtcgccgta tgataggcca agcccgtttc aaaggcatgg tggttggtct
    36121 tggcagctgg ataggagtag aattccttat catacttggt gtagagattt cggacaatcc
    36181 gttgccagac aggattttca attttgaaaa tcatttgcga catgtagtca cgaatttcct
    36241 tgacatcaac tggtgacttg accttgaaat cggctgggtc attgggttca ccagcttgag
    36301 gcaggcggag agtaatttga ttgacttgag gggtattgtt ataaacttct cggcgtcctt
    36361 tcatgtggac aaccttacct gcggtaaagg cctcaatgtt atgaggttgg gcatcccaga
    36421 gcttcccatc aatctcgcca ctatcatctt ggaaggtaaa ggctaggtag tttttcccag
    36481 ctcgagtttg cctcaggtca gctgatttga ttaggtaaaa gccttcaaat agctcatctt
    36541 ttttcatgtg actaatcttc atattcttcc tcattttctt gaaaatggag tagatcaagc
    36601 gcaggctcgc cttctgacaa ctcaatgtga cggagcgtcc gctcgatagc tatggtacga
    36661 cggtttaata attcatcaat attgccagag gcatgttgga gatgtttttg tgccttgacc
    36721 agaataccac caaacttgcc aaactcggtc ttgacactgg caagagtctt gctgatatgg
    36781 tcggcactct tttggatatt aagggtcttg aaaccaactg atagggagtt aagaagggct
    36841 gatagggtac ttggtcctgc aacgataatc tgttcttccc gtctcaaatc atcaaagaag
    36901 accggattgc gcacaatctc tgaataaaga ccttctgtcg gaacaaacaa aactccaaaa
    36961 ttggtcgtcc gaggcggtgc tatgtacttg ctcttgatat ccttggcaaa gcgcttgacg
    37021 cttgctagga gtgacttacg acagcgttca atctcatcct tgtcacctgt ctcataggct
    37081 tcttccaagc ggtaataatc tgccagtgga aacttagagt caattggcag atagacgtat
    37141 tcttggtcgc cttgtccggg taacttgatg gcatactcca ctcgttcact agagttttca
    37201 accgttgcgt attctcgttc gtactgggca ggtgtcatga tgtcttcaat aatttgcccc
    37261 agttgcaatt ctcccagaat ccctcgcgtc ttggttccag agagaacctt gttaagagct
    37321 ccgacatcac gggcaactgt ctgcatttct ccaaggccac gattgacaga ctccagttgt
    37381 ttagaaactg tctcaaagga agcctgtaag cgtgtctgca aggtcttttc tagtttttcc
    37441 tcgaccgtct ggcgcatttg ttccaaacgt tgttcatttg attcctgcaa ggcttggaga
    37501 cgttggtctg tcttgtctct agtttggagg agattatctg tcatttcttg acggacttgg
    37561 gtcagacctt ggtgcaattc aatccgcact tcttgcaaac ggtcgctgac aaccacttcc
    37621 aaatcttttt ggtctaactg gctggcttgt ctggcttggt caaagcggta atccaactgg
    37681 tctgacaaat gatctgcctg atcctccaag ctcttactta agtgtttctc ctgcctatcc
    37741 tgcctttgcc aaatcagaaa gagaccagct aggttggcaa ttaataatag aataagtaaa
    37801 ctttccatcc tacctcctgt ccttgctatg cagtacgacc acataaccat ctgggcaagt
    37861 taccgacact tccctatcta tatattcgtt agaagcgtac acttttttaa agaaaaaatt
    37921 ttcctctgtc aactcatact tggctccaag aatagtcagc tggctatccc gaactggcat
    37981 aaaggctaga tagtcgtagt ctgaacgagg ttctagctga ctggtccctt ctggacaata
    38041 agtaatcaag ttttgcccat cctcaatttc tatttgatgc atatagggtg ccaacttagg
    38101 attgctaggc agaaagacat tggccaacat atggtcaata cggccaccca aggcaccgaa
    38161 aatagtaacc tgagcctgag gatttttctc aaagatggtt aagagagcca attccaaatc
    38221 ggtatcatct ttttctggtc gggcttggac aaaatgctgg gcacgttttt gaatcacctg
    38281 tcgctcttct tcggtcacag aatcaaaatc cccaactgct agagcaagaa gcaagtcttc
    38341 ttccaagacc cagagcgagc ctcgatccac cccaacaaaa gcatcaaaat ctgtccgata
    38401 atgaccgcgg tctccgcctg caaaaacggc aaccctagtc cagttgtttt ctgagagttt
    38461 gtactcgctc attgacatct cccttaaaga cataggaacc tgctacaaaa acggtcgcac
    38521 cagcttcttt ggattgagca atagtttggt catcaatccc accatccact tcaatctcaa
    38581 agttcaaacc tttttcatca cgaagggcaa ccagctcacg aaccttatcc atggtttctg
    38641 gcagaaaggc ttgaccacca aatccagggt taactgtcat gactaggact tggtcaacta
    38701 gatgaagcac atgcttaatc gcttcaactg gtgtaccagg attgataaca actgatggct
    38761 tgacaccgag tgaacgaatc ttttggaggg caccatgaat atgaggcgtt gcttccacat
    38821 ggatactaat aatatccgca cctgcacgca caaagtcctc taaatgatgc tcagggtttg
    38881 acaccatcaa gtggcagtcg aaaaccatct tactatgagg acgaagagct tcgaccacac
    38941 ccgcaccaaa actgatttgc ggcacaaaat gaccatccat aatatcgata tgggcatact
    39001 ctgccccagt tgcttctagg cgtttaattt cacgttcaaa gttggcataa tctgctgcca
    39061 gaattgacgg agcaatcttg tattgagaca taggtttctc cttattttgg aatttttttg
    39121 ctgacttttt tataggtttc tctgcgattt tcaatttcgc tgaggaattg caggtagttg
    39181 tcaaaacgga agggtgcaat ggtaccttct tcaactgctg gcttgacagc acaggacggc
    39241 tcatgggtat gggtacaagt acggaatttg cagtctcgac tgacagtggc aatctctgga
    39301 aaggcctgat tgaggtcttc agccgttgat acttcataat ccagtgatga aaagcctggt
    39361 gtgtctgcga ttttaccccc attgaggttg taaaaactaa cagctcgagt ggtatgacgc
    39421 ccgcgaccca gactgtctga aatttctccc gtttcaagat taagatctgg tgcgatttta
    39481 ttgagaagag ttgatttccc aacacccgtc tgccccataa agaccgtaac cttgcctgtt
    39541 aacaaaggca ggagttcctc tttactggtc acaaagtcat atccgatgtc accataggtc
    39601 tgctggtaaa aatccagttc tcccccgtct tccaacaaat ccattttaga aatatagacg
    39661 atgggatgaa tacccttatg ctccaaaaga accaagaaac gatccagcaa attgctgtta
    39721 aaatcaggtt ccttgacaga catgattact acagcttggt cgatattgac aataggcgga
    39781 cggaccagac tgtttttccg ttcgtgaatc ttgagaatat agccttctga attttcctcg
    39841 gcagagaaat ctacccagtc cccaacgtaa ggggtatgcc cttttttacg gaaattccca
    39901 cgcgcgcgtg tttgatagac ttggccatca ctctctacat agtagaaccc agccaaggct
    39961 ttaatgattt gtccctgcaa aataactcct ttaaaattgt tagttccatt ataacaaaaa
    40021 aaagctattt tagcttagct tctctttaca atttcgataa gtctattcta aaagaaaaag
    40081 atacactcac gctttagata ccaagtttta tacaaaaata acgaatctac ctccttatat
    40141 aaaaacaggc gaacagagag cctaaaactc cctgttcgct ttactttttc tgtgtgtaat
    40201 agaaaaaacc taataaagca agacaaaaaa tatgaatata aactccatac atcatgtggg
    40261 gaaagacaga ctctataaaa tagcctggta gatagatgat tggaaattga tagaaatcat
    40321 aggtcgttag agatagtaaa caaagaacat acataaatac tgctacaatt agaacccatc
    40381 cttgctttcg ccatagaaaa atcatcaata agaacagaca aagtagatag attgaccaat
    40441 ttcgcaacat aaattgaaaa ccataaatca tactttcagt cgtttctcca tcaatcacat
    40501 caactacttt atagaaaggt aaacaatagc tcactaaaaa aagaagggga ctaattattt
    40561 ttttcatatt ctctaccatg gattattttg caagtaagct gaccatctat ctccatatcg
    40621 aatcaaatta acagtataat tagttactac ccgattttca cctggtccaa ccttagaaaa
    40681 acctgtgcta tactcccaac tacttcttgc acctggatga aaccattttt gctgggatga
    40741 ataccaacca ccttgtctaa cttccacact tgaagattct atttcgatat ccctttcatg
    40801 gaagtcaact cctccactta ttttagtaat atttttttgt acacctccat cataactcac
    40861 ccagtgaaca acagtataca attccacact ataagtacta tcaaactgac tttgatgagt
    40921 agtaatgttt tgagtataag cgtgcacttt ctgtatacca aaaatatggt ctaatatatt
    40981 cgttggttta gcaagcttat tgtaaccctc ttcaacaaaa gtagccttct ctaaatcagc
    41041 ggtataaaca gtttttcctt gtactacttg attgtttata gaagtaatac ggctatccac
    41101 aacaatgggg gccaccatat ctccatcgct agtttctatt ttagctgcct ctttcacctc
    41161 atatacaact cgattttctt cagactgaat cacttctacc ccattttttt caggtataga
    41221 taatctagtt tgcccattag aaaccgttat atttggacca atattcttct catttgcata
    41281 agtcgcaata ggaaatcctg atagaaccac tgcacacata aggctattga ctaatttttt
    41341 tctcatgaat atatccttcc cattttcaag aggagtaaaa atagctaata taagatgaac
    41401 gtttccttat atttcagaat aggatatcta tctgaaagca attcacactc ctataggtaa
    41461 ctgttccaaa tcttggctac atatatttca atagtttgct acaaaaaact cctttctaag
    41521 tctattgaac atgcccttat tcacctacta atatatcaca aaaataaata aatacaagac
    41581 tgttttttct actagtttta tgatagaata gtattgttag ggtaaatttc atatataaat
    41641 gtcgagtgaa aaatcgatgg agcagaaatc ggtagacaaa gactcgattt cgttggtcca
    41701 gccccacaag gctaatcagg tatttcacga gtttaaaaat ctaatgaagt gcccaatcaa
    41761 ctactgattc attgatttaa tacgaactat agtggattga acctagaaat agtacactaa
    41821 ggattctaaa acgtttctag aaattaattt gactttccta atctctttgt tcatatctta
    41881 tttcaatcta ctatatatac caaaatgctg ggacaaaagt ctttaaaatc agagaaacgc
    41941 atattatcag gtgttggaaa accttgataa tatgcgtttt atcatagaaa gatctacttc
    42001 attttttacc aaaattaagt ttttgtccct gcatcttaga gtccttgccc tttaagtgca
    42061 tctgctaggc tggcaaactc ttccaagctg agagcttccc cacgcacact tggtgacaag
    42121 cctgcttggt ccaaagcctt ggtcagcttg tccttgacct cttcagtctt gccaaagtaa
    42181 cctgtcaagt tattccacaa ggtcttgcgg cgatgggtaa aactagcctt ggaaacctta
    42241 aagaaaaagt tctcgtcttc cactgctaca gctggcactg gacggcgcac cattttcaag
    42301 atggctgagt ctacatttgg tgctggcaca aagactgtac gcggcacgat aaaggcaacc
    42361 ttggctgtca tgtaatactg cacagcgata gacaagctac cgtaagcctt ggtatttggt
    42421 tgagccgaga tacggtccgc cacttctttt tgcatcatga ccacaaactc actaaaagga
    42481 atgccactct cgattaagtg catgagaata ggcgttgtga tgtagtaagg caaattagcc
    42541 actaccttga ttggcaggtc aggatttttg aaattctgga tatgttgcgc caaatcgacc
    42601 ttgagaatgt cctcgttgac tacagtcaca ttgtcaaaat cacgcaaggt atctgccaaa
    42661 atcggtacca aacgatggtc gatctcaaag gccataactt ctgctgcacg ctcagccaaa
    42721 aactcggtca aggcaccaat acctggcccg atttcaatga cattgacctg gtcatcaatc
    42781 tcagccgtat ccacgatttt ttgtaggata ttggtatccg tcaagaaatt ttgcccaaag
    42841 gactttttaa aggtaaaacc gtgacgctcc agcactgcct tggtcacgct ataatctgca
    42901 attctcatct attctctttt ctattatctg ttactactat tgtaacacat tccccttgta
    42961 ttttgggggt gaaaatcata ccaaaaaact atctttcctc atgaagaaag atagtcttgg
    43021 aaatctttaa aagtggcctt agcgcttctc tcgaaacata cgtt
//
LOCUS       DAVBDG010000010        42876 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBDG010000010 DAVBDG010000000
VERSION     DAVBDG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.010"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..1954)
                     /gene="ftsH"
                     /locus_tag="VQ526_000488"
     CDS             complement(<1..1954)
                     /gene="ftsH"
                     /locus_tag="VQ526_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2554253.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNREIHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
     gene            complement(1970..2512)
                     /gene="hpt"
                     /locus_tag="VQ526_000489"
     CDS             complement(1970..2512)
                     /gene="hpt"
                     /locus_tag="VQ526_000489"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2554254.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2516..3793)
                     /gene="tilS"
                     /locus_tag="VQ526_000490"
     CDS             complement(2516..3793)
                     /gene="tilS"
                     /locus_tag="VQ526_000490"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2554255.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3790..5058)
                     /locus_tag="VQ526_000491"
     CDS             complement(3790..5058)
                     /locus_tag="VQ526_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2554256.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5178..5546)
                     /locus_tag="VQ526_000492"
     CDS             complement(5178..5546)
                     /locus_tag="VQ526_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2554257.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5539..5805)
                     /locus_tag="VQ526_000493"
     CDS             complement(5539..5805)
                     /locus_tag="VQ526_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2554258.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5863..9372)
                     /gene="mfd"
                     /locus_tag="VQ526_000494"
     CDS             complement(5863..9372)
                     /gene="mfd"
                     /locus_tag="VQ526_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2554259.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            complement(9373..9942)
                     /locus_tag="VQ526_000495"
     CDS             complement(9373..9942)
                     /locus_tag="VQ526_000495"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2554260.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(10013..11128)
                     /gene="ychF"
                     /locus_tag="VQ526_000496"
     CDS             complement(10013..11128)
                     /gene="ychF"
                     /locus_tag="VQ526_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2554261.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11212..11406)
                     /locus_tag="VQ526_000497"
     CDS             complement(11212..11406)
                     /locus_tag="VQ526_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2554262.1"
                     /translation="MYQVGNFVEMKKPHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11471..12607)
                     /gene="dnaN"
                     /locus_tag="VQ526_000498"
     CDS             complement(11471..12607)
                     /gene="dnaN"
                     /locus_tag="VQ526_000498"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2554263.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12766..14127)
                     /gene="dnaA"
                     /locus_tag="VQ526_000499"
     CDS             complement(12766..14127)
                     /gene="dnaA"
                     /locus_tag="VQ526_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2554264.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14340..15098)
                     /locus_tag="VQ526_000500"
     CDS             complement(14340..15098)
                     /locus_tag="VQ526_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2554265.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15156..16337)
                     /locus_tag="VQ526_000501"
     CDS             complement(15156..16337)
                     /locus_tag="VQ526_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2554266.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGKIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16520..16999
                     /gene="rlmH"
                     /locus_tag="VQ526_000502"
     CDS             16520..16999
                     /gene="rlmH"
                     /locus_tag="VQ526_000502"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2554267.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17043..17116
                     /locus_tag="VQ526_000503"
     tRNA            17043..17116
                     /locus_tag="VQ526_000503"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17077..17079,aa:Arg,seq:tct)
     gene            17281..17406
                     /gene="comC"
                     /locus_tag="VQ526_000504"
     CDS             17281..17406
                     /gene="comC"
                     /locus_tag="VQ526_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2554268.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            17427..18752
                     /locus_tag="VQ526_000505"
     CDS             17427..18752
                     /locus_tag="VQ526_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2554269.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18749..19501
                     /locus_tag="VQ526_000506"
     CDS             18749..19501
                     /locus_tag="VQ526_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2554270.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19553..19624
                     /locus_tag="VQ526_000507"
     tRNA            19553..19624
                     /locus_tag="VQ526_000507"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19586..19588,aa:Glu,seq:ttc)
     gene            19630..19703
                     /locus_tag="VQ526_000508"
     tRNA            19630..19703
                     /locus_tag="VQ526_000508"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19664..19666,aa:Asn,seq:gtt)
     gene            complement(19744..20286)
                     /locus_tag="VQ526_000509"
     CDS             complement(19744..20286)
                     /locus_tag="VQ526_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2554271.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20465..21232
                     /locus_tag="VQ526_000510"
     CDS             20465..21232
                     /locus_tag="VQ526_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2554272.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21236..21655
                     /locus_tag="VQ526_000511"
                     /pseudo
     CDS             21236..21655
                     /locus_tag="VQ526_000511"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21683..21997
                     /locus_tag="VQ526_000512"
     CDS             21683..21997
                     /locus_tag="VQ526_000512"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2554273.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(22098..24650)
                     /locus_tag="VQ526_000513"
     CDS             complement(22098..24650)
                     /locus_tag="VQ526_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2554274.1"
                     /translation="MKSFLKTYRTYFISLIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDILAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(24712..26334)
                     /locus_tag="VQ526_000514"
     CDS             complement(24712..26334)
                     /locus_tag="VQ526_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2554275.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            26540..27565
                     /gene="trpS"
                     /locus_tag="VQ526_000515"
     CDS             26540..27565
                     /gene="trpS"
                     /locus_tag="VQ526_000515"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2554276.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            27717..29195
                     /gene="guaB"
                     /locus_tag="VQ526_000516"
     CDS             27717..29195
                     /gene="guaB"
                     /locus_tag="VQ526_000516"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2554277.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(29246..30343)
                     /gene="recF"
                     /locus_tag="VQ526_000517"
     CDS             complement(29246..30343)
                     /gene="recF"
                     /locus_tag="VQ526_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2554278.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(30346..30714)
                     /gene="yaaA"
                     /locus_tag="VQ526_000518"
     CDS             complement(30346..30714)
                     /gene="yaaA"
                     /locus_tag="VQ526_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HET2554279.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            30873..32123
                     /locus_tag="VQ526_000519"
     CDS             30873..32123
                     /locus_tag="VQ526_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2554280.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAELEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            32120..33403
                     /locus_tag="VQ526_000520"
     CDS             32120..33403
                     /locus_tag="VQ526_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2554281.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGYVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTIDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            33445..34266
                     /locus_tag="VQ526_000521"
     CDS             33445..34266
                     /locus_tag="VQ526_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2554282.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            34277..34822
                     /gene="pgsA"
                     /locus_tag="VQ526_000522"
     CDS             34277..34822
                     /gene="pgsA"
                     /locus_tag="VQ526_000522"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2554283.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            34819..35646
                     /locus_tag="VQ526_000523"
     CDS             34819..35646
                     /locus_tag="VQ526_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2554284.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            35631..36470
                     /locus_tag="VQ526_000524"
     CDS             35631..36470
                     /locus_tag="VQ526_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2554285.1"
                     /translation="MGIALENVNFIYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            36463..37257
                     /locus_tag="VQ526_000525"
     CDS             36463..37257
                     /locus_tag="VQ526_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2554286.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            37316..38134
                     /gene="mreC"
                     /locus_tag="VQ526_000526"
     CDS             37316..38134
                     /gene="mreC"
                     /locus_tag="VQ526_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2554287.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            38134..38628
                     /gene="mreD"
                     /locus_tag="VQ526_000527"
     CDS             38134..38628
                     /gene="mreD"
                     /locus_tag="VQ526_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2554288.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            38722..39900
                     /locus_tag="VQ526_000528"
     CDS             38722..39900
                     /locus_tag="VQ526_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2554289.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            40124..40903
                     /gene="rpsB"
                     /locus_tag="VQ526_000529"
     CDS             40124..40903
                     /gene="rpsB"
                     /locus_tag="VQ526_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2554290.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            40982..42022
                     /locus_tag="VQ526_000530"
     CDS             40982..42022
                     /locus_tag="VQ526_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2554291.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            42268..42555
                     /gene="tnpB"
                     /locus_tag="VQ526_000531"
     CDS             42268..42555
                     /gene="tnpB"
                     /locus_tag="VQ526_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2554292.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVD"
     gene            complement(42746..>42876)
                     /locus_tag="VQ526_000532"
     CDS             complement(42746..>42876)
                     /locus_tag="VQ526_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2554293.1"
                     /translation="IRKRGFKNKAFRTLEDVIQGLEKEVIKSIVNRRRTRMLFESR"
BASE COUNT        13431 a         7400 c         7899 g        14146 t
ORIGIN      
        1 tttcgtcatt catttttgac tttacttcat cataggatag tgcatgagat tcctcttcta
       61 ctgcttcagg catctttcct gtttcgtaaa gagctttaat ttgtgtacta tccaatgttt
      121 cgtatttcaa taatgcttct gcaattaact tgtgaatttc acgatttgac tgaataattt
      181 cagcagcttt atttcgtgcc tcatttaata atgaacgaac ctcttcatca atttcataag
      241 ctgtttgttc tgaaattgat ttttgaggac tctgtgcacc aaacatagca tggtttcctt
      301 catattgtac tgggccaagt ttttcactca taccgtactc tgtaaccatt gcacgcgcca
      361 tttgtgtcgc ttgttcaaag tcgtttgaag ctcctgtagt ttggacatta aagataattt
      421 cttcagctac acgtccaccc attaagccag ccaattgctc tttcatatct tctttagata
      481 gaagcatttg atcctcttta ggaagtgcaa tcatgtatcc gcctgcacgg ccgcgtggta
      541 caattgtaac cttatggaca acgcgagcat tcgacaagac tagaccaaca atggtatgtc
      601 ctgcctcatg gtaagcaacc aattctcgtt ctttttgtga aactgtctta tctttcttag
      661 aaggtccagc aataactcta tcttctgctt catcaatatc tgaagcatca attatcgatt
      721 tattgcgacg agcagcaact aaagctgctt cattcaagac attctctaaa tcagcaccaa
      781 caaagcctgg agtttgttga gccactaatt tcaaatcaac atcttctgct aaaggcttgt
      841 tcttagcgtg aactttcaag attgcttcac gacctttaac atcaggacga ccaaccaata
      901 cttttctatc aaaacgtcct ggacgcaaaa gggcaggatc aagtacatct gaacggtttg
      961 tcgcagcgat gacgataatc ccttcatttc cctcaaaacc atccatctca atcaaaagtt
     1021 ggttcaaggt ttgttcacgt tcgtcattac ctccgccgag accgactcca cgttgacgtc
     1081 caacagcatc aatttcatcg ataaagatga tagctggtgc tgcttttttg gcatcctcaa
     1141 aaagagagcg aacacgacta gctccaactc cgacaaacat ttctacaaag tcagaacctg
     1201 agatactaaa gaatggaaca cctgcttctc cagcgactgc cttagcaagc aaagttttac
     1261 ctgtccccgg aggtccctcc aaaagaacac ctgctggaat acgggctcca agttttgtga
     1321 atcgttttgg atcttttaag aactcaacaa cttcaactag ttcttgtttt tcttcctcag
     1381 ctccagcaac atctgaaaat cttactttaa tatcttcttt atttgctgct ttagccttac
     1441 tacgtccaaa actcattgga ttacggccat tgcctcctcc catatttccc atcatagaga
     1501 ataggaagaa gaatagaatt ccaaatggca caatggatac gagtagatta atccatatac
     1561 cacttgaact ttcatgctta acagttactt ctgctttatg gtcagtagca agtttttgca
     1621 attctgatac ggtagtatct gcaggaagaa tagtgctggt aaatttctct accttagtaa
     1681 cagatggcgt gaaaaactga atacctgttt cttctttact tgttttagga tttttataga
     1741 caccagaaac ttcgataaca ctaccatttg gttggtaagt taattctttt acattaccat
     1801 cggtaatttc ttgtaccaac tcagtatagt tgatttgctg acttcctcct gagttattcc
     1861 cagaatagaa atactggaat cctgtcacaa ggaaaaagat aaataataac catagaaaag
     1921 gatttttaat taaaccatta ttttgttttt tcattaaaga ttattctttc taatttgaat
     1981 acacttcctc tttcaatact ccaatataag gaagattacg ataattttct ttgtagtcta
     2041 aaccataacc tactacaaac tcatttggga tagtaaagca ggtatagtct gcctcaattt
     2101 ctacaacacg tccttctggt ttatccaaca aggttgcaat tttaacagaa gctgcttctc
     2161 ttgctttaaa catatctcgc aaattcttca aagtttgacc tgtatcaatg atatcttcta
     2221 caaatagaac atgtcttcct ttgatatctt gagtcacatc ttgtttaata ttgataacac
     2281 cactacttgc tgttccacca tggtagctag aaaccatcat gaagtccatt tcaatatgtg
     2341 tatcaatatg tttgaccaat tcagccataa aaggaataga tccttttaaa atcccaacta
     2401 agattggatt ttttcctgca tagtctttag ttaattgagc acctagtttt ttagctgctt
     2461 ctgtaatttc atcgtgtgaa acgaggactt ttttaatatc gttttctaac attttttacc
     2521 tatctatttt ttctatataa agtacagtgt tcattatatc atttttcgtt tttttactca
     2581 aattattggt cgcaattccc aaaattgaga caatttcacc aaattgctca ataataagag
     2641 cagagtttct cttttccata gggattttca aatcaataaa taaacgtctg agtttttttc
     2701 tatgcccatt ttgaatcaaa acatctcctg tttttcgatg acgaatgtgt atggatgttt
     2761 cacgtgaaac aggtatttgt tgaattgatt caccttctaa tggaagtcca aaggaaaata
     2821 aatatccttg ataagctacc tgattttgat agtgtaacac aagttcatct tcctcttcat
     2881 cagcctgcgg actgatttta caaatctgaa actgttggta ctcttttatc aattcatagc
     2941 catttttaat cggatgacga tactggcttt tagattttaa aatctgctga acttcagcaa
     3001 actgagcttt tgtaagattc aaatctggaa aacgattcag ataagtttga agtaaaactc
     3061 tttgtgtaga ctcagagtaa gaaaataact gctgtaaatc ttccacatta atattgttag
     3121 ataattcagc tattgccaaa tcataatcta aaatttcatt gccaatgcct aagattgcat
     3181 ccctaaatcg aggattttct ttttccaatt ctggtaagta agagtttcga atacgatttc
     3241 gaaaataatg attctcctga tttgatgtat cttcaaagtg aaaaattgat ggaaagtctt
     3301 ttttctgaaa atgcaagaag ggacgaatga tttctatctc tccgactact tgcttctcct
     3361 taattcctga tagatagcgc aagcgagttc ctcgaatcaa gcgcatcaaa atcgtttcca
     3421 cctgatcatc agcatggtgg gcagtgacta aagctgtcgc acctgtcttt ttcatgacct
     3481 cttgaaaaaa atcataacga aaatttcgtg cacgcgcttc tgaaaattct cctgaaaaat
     3541 tgctgatata aataggaagc tctgcttcag cagccaactt ccttaattcc ttttcttccc
     3601 aatctgattc aattctctgc ttatgattca catgagctag aatcaattca atctctaact
     3661 ctttttgata agtagacaat accttaaata gaaacatgga atctaatcca ccagaaagag
     3721 ctagaaccgc cttagcatgc tttttgaaat atcccttctt gagaaaatga tttaaaaaat
     3781 ctggttccct cattttagaa cctcataaac atccttggct atcttagaaa tcgtatcata
     3841 atcagaattc ttagtgaaaa tagaaagaat aaatggagaa tctgcataga caacacccgt
     3901 atcatgctta aattcatccg catctccaat tttatgagct actttaacag aaacaccttt
     3961 ggcaattcgc tgactatcaa aatctgtttt agtcaaagac tctaacacaa atccattttg
     4021 attataaata gcttccataa acttcccggc catcttagaa gaaatcaatt tttcttttgg
     4081 atcccaatca tctcccataa tggcagacat cttggatttg aatgtggcat cagattggtt
     4141 tgaaatgtaa tatcccaata gattatgagc tacattatca gattcttttg atactttcgt
     4201 aattaaatcc tttaaagaat attctttatt atcttctttt ttaggaagac taccacttcc
     4261 ctctggttta taagaacctg gaaaatcatt gactgcagat acgtatttta cagtcgtatc
     4321 taactgataa agaccctcat ttattttttc ttgcgtataa tagagataag agagtttcaa
     4381 aacgctggct gcatacatct tttcatcttg attgatacca gcttcttttc cagtagtcag
     4441 ttgcttaaca taaatagaga aagaatcttt ctgatatttt tcagataaca tttcttgaac
     4501 tttactcatc cgattatctt cttcagaagt tgattcttta gctacccatc cagcctgatc
     4561 aatatgtaga aattctcttc cttctacaaa catggtcttg tcgattgata cttgcgaata
     4621 agctgataag gatgatttca cttcttttaa atcataagga ctattgtaca gtttaaagtc
     4681 agattctaac catgcttttt ttattgttgg agttacctct gattgatcat ataaaaatcg
     4741 tttgtccgca gctataaatt gatgatttga tagcttaaat actggaattc cttgtttatt
     4801 taagcgccac tcggttattt gaaaacttgt tttgggagtc aatttcccag attccactac
     4861 taaatcttca ttcgcataaa caggaacctc tccataaacc atgggagaac ttaatttttc
     4921 tctaaaataa ataccaaagt cagattgtga aaggtaataa atttctttcg aagtatagac
     4981 gacttctttt tctgtgctaa cgacttttga aatggtcaaa aaacttggta gcaacaaaat
     5041 aattaagaat ttacgcattc ttcctttcct tttcttcttg taaacgcaat aattgatttt
     5101 tatcagccaa ttcttccagc ttttcatctg acaaactcaa aaattgctct attacgtcta
     5161 ataaattttc cattttatca cctttgaagc aagtcaggaa tcgtataaac tttttccctc
     5221 gacttagaat aatagtactt cgctcgtgta tatttggcag cataatcttc atctttcaac
     5281 ttggtagcaa atgctgtctc cttatccttt tcatcactca aagtttgata ctgagtttgc
     5341 aagtctgcta attgctgacg tctttggagt aattgctgat aactctgcgc taaattaaaa
     5401 gttggcaaga taaataatag cataatcaaa atcaataccc ctcccataaa acgattccgt
     5461 ttttgtcgtt ctttcatcag gtagcgacga cgttggtatt cattttgaat aaaagaatta
     5521 ttcaattgta caatattttt agacattttc ttctacccgt gtttcactga taatttcata
     5581 cattcctgct gcatcttctt tttttgtact atctttcatc tctagtactt ttacaagcag
     5641 caacttattg ccaaagcgaa tttcaacttg gtcattaact ttcaagtccg ttgaactttt
     5701 ggccaagatt ccattaacct tgattctacc tttatctgct acttcctttg cgactgtacg
     5761 acgcttgata attcgcgata cttttaaata tttatctaat ctcatcctta ttacctcaat
     5821 ttattattgt accattttta tctattttat agaagaaaaa tatcaaatgg aattttcttc
     5881 cttagactct tttatctcta ataaactttc tccaaaaatc agcagacctt ctaaaatttc
     5941 ataatctttc ttattttgga catcaaatac aagctccatt aatcccttat tctcagcgat
     6001 gcctgctttt aagttcgtta cggataaagc tttaaaataa tcttgagcta aaaacaatcg
     6061 ttgagtgact ttttcaaatt gaattgtaat tttattatct tttctttcca cacgttgaac
     6121 aaagaccttg tccaagtatg atttaaccaa accaatctct aaaagatagg ctactacatc
     6181 tgggtattct ccaaaacggt ctatcaactc ctcttgtaac tcttcataat tgacacggtt
     6241 gtcaatttga cgaattttct tgtaaatttc aatcttatgt cgttgatcag aaatataagt
     6301 atcaggaaga taggcatcaa tttgcaaaat caactcagca ttccctttgg ttcttgtgtt
     6361 agcgttagca ttaccgtttc gtttagcaat agcttcctct aataactgcg aatacaattc
     6421 aaaaccaaca gaatcaatga aaccagactg ggattttcct aaaagatttc ctgctccacg
     6481 aatcgaaaga tctcgcattg caatcttaaa gccagagccc aattctgtaa atcctttaat
     6541 cgcttctaat ctcttttcag agacttcact gattgatttt tctggacgat acatgagata
     6601 agcataagca atacgattac tacgaccgac tcttcctctt aactgatata aggttgacaa
     6661 gcccatatgg tccgcatttt caataaataa agtattagca tttggaatgt ccacccctgt
     6721 ctcaataata gtagtcgtca ccaagatatc gtattgtccc tcaataaagt ctaatagagt
     6781 attttccaac tggacttcac tcattcgacc atgaacatat ccaatcgaag cctctggaat
     6841 caactcctgt aattctgaaa ccttctgaac aattgtatca actttgttgt aaagataata
     6901 aacttgacct ccacgctcca tttcacgcaa gacagcatca cgaatgacac tatcattctt
     6961 ttccaaaaca taggtctgaa caggatagcg attagtcggc ggagtttcaa taacagacaa
     7021 atctctgatt cccagcatag acatatggag ggtacgaggg attggcgtag cggtcaaggt
     7081 taggacatcc acttgtttct tcagttcttt caaagtttcc ttatgcttga caccaaatcg
     7141 ctgttcctca tcaataatca tcaagcccaa atcagcaaac acaacatctt ttgacaaaac
     7201 acgatgtgtt ccaatcaaaa tatcgacttg accgtttttc aatttttcaa gtgttgcagt
     7261 ctgctctttt ttacttctaa agcgactcaa cacatcaaca ttaactgcaa aattttggaa
     7321 tcgttcctta aaattcgtat agtgctgttg cgctaaaacc gtcgtcggaa ctagaatgac
     7381 aacctgtttg tgatcattga ctgctttaaa ggctgcacgc atagcaactt cagtctttcc
     7441 aaaaccaaca tccccaacta aaagtctatc cattggctga gaagcctgca tatccctctt
     7501 gatttcctca atactacgaa gttggtcatc cgtttcaaca taagggaaag catcatcaaa
     7561 ggcatcttga tcatcatcat cagctgagaa agcaaaaccc ttcaactgac tacgttcaga
     7621 gtagagtttg attaaatcat cagctatatc ctctacctgg ttcttaacct tttgcttggc
     7681 ctttttaaaa tgaccgtcat ttaatttatt gagttttgga gctttaccat cacttgaaat
     7741 atatttggac agtagatgaa tctgttccac gggaatagaa atttgatcac cattttggta
     7801 ttggacactg acataatcac gatgaattcc cttgatttca atggtttcaa ttcctagata
     7861 ttgaccaatc ccatggatat gatggacaac atagtcccct ttttcaagtt cattgtaatc
     7921 ttttaatctc tctgcatttg aaacatgttg tcttcgaaaa cgacgcttta atttcttttg
     7981 aaaaatctca tgttcagtta tcaataaaat cttttcatct acaaaatgaa aaccatgtct
     8041 gagattacct tctattaagt ttacagattc tttacagata cttgtcttat ctctagaatc
     8101 caatttaatc tgatattcct ctaacatatc ctccaatgtt ttacttccca ttgaattgct
     8161 agactgcaga ataatggtgt aatccatttt tttatatcgt tcaatttctt cttttagaaa
     8221 agaaaactga ttgaaaaatt cctgcatagg atattgattg aattgataaa ttttgtcaaa
     8281 tttgagattt cctaaaccct tttgaagatt agagaaaaag gtcactggac tttgtttttt
     8341 atagatttgt tcaatatcag aaaaatactg catatcagaa aatgctttac tattctgtaa
     8401 ttcttctgta aagtactgcg ctaattctct ttcaaagact tcatactgat tcatcaattt
     8461 ttgataatca tcaaagaata ttggagtatc tttttcaata taatcaaaga cagtccatgt
     8521 cttatcatag cacaaagata aaaacttccg agaatctgca tgactttgct tttgatgaaa
     8581 acttgaaaga atttcttcta ggtatgattt caaaataggt gataaagttt ttgaaatttg
     8641 tttttctaaa gctgactgtc ctcgttgata atccttttct ctcaaaagca tatcactagc
     8701 tggaaagata gtgagttctg tcttattttc tttcgataat tgtgtttcta cttcaaatga
     8761 cctgatacca tcaatttcat caccaaaaaa ctcaattcga caaggttcta actgggatat
     8821 ttcaaaaata tctaaaatat ctcctcgaag actaaattca ccctgagttt gtacttgagt
     8881 aacttttcga tagccatttt cctttaactg atggataaac gcgtgttgag catattcttc
     8941 accaactgag atttttacaa tactatcttt gaatgcattg ggagacggta aaatcaatcg
     9001 acttgctgcg atattacaaa ctaaaatccc tttcttagat gaatcagtca aaaaacgcaa
     9061 ggcttcaacc cgtgaaataa ttttttcctg tgaagacatc aaaaactcca ccataggagc
     9121 atcatctacc aaaaatggat agacgagttc ctcacccaag atagaaataa gatcactaac
     9181 aagtccttct gcttctccat aagttgacgt caataacaca atcctatctt ctttttctaa
     9241 actgcttgca attgcaagag ccttagtaga tgttgataaa ccaagtatta gttgtctttt
     9301 cttatctgtt aaattttgat gccatttttt aatctgatca ttttctgaga ataaatctaa
     9361 taaggtcacc atttatccgt tatacctctg cattgttttc tcaaaatttt tctcttgtaa
     9421 atagtagttt acagaatcgt caactttgtc aacagactgt aaaataccga tataatcatc
     9481 cctgtcaaac ttactcaaaa catgatgaac aactgacata ccatttttag gtcttccaat
     9541 tccaatctta acacggttaa agacctgagt tcctatatgt tgaataatag acttgatacc
     9601 attatgacca cctgctgagc cttttgctct taaacgaatt ttcccaactt ccatgtcaag
     9661 atcatcgtaa atgataagta aatcgtcaat atccaaacca tagtaagtta ataaagcatg
     9721 aactgctttt ccactttcat tcataaaggt cgttggttta accagataaa ttttttctcc
     9781 atttaggaaa aaggatgcta ggtcagcttg aaatatctta tcgtgtgtaa aagtgacatt
     9841 ctgtttcttc gctagttgat caatcaacat aaaaccaaca ttgtgttttg tttcaaaata
     9901 tttatcccct ggatttccca agcctacaag taatttggtc atttattttt cctttcaaaa
     9961 gccaaaaggg ttggaatttt tttccaacct aattgacacc atttattaat ttttagacat
    10021 taaagcggaa ttccatgata tcgccatctt gaacgatata ttcttttcct tcttcacgca
    10081 agcgtccagc ttcttttacg gccttttcag atccgtattt cactagatct tcatatgaca
    10141 tggttactgc acgaataaag cctttttcaa agtctgagtg gataatacca gctgcttgag
    10201 gagccttcat accacgtttg aaagtccaag cgcgaacttc tttttcacca gctgtgaagt
    10261 aagttcccaa tccaagcaag tggtaagctg cacgcgtcaa cttatctaca cctgattctg
    10321 tcaaaccaat ggcttcaaga aactcttttt tatcttcatc atccaattca gaaatttctt
    10381 cctcagcacg cgcagaaata acgactactt cagcattttc tgtcgctgca aattcacgaa
    10441 tttgtttgac atagtcgata gagtcaggtt ccgaaaccac atcctcatcc acattagcta
    10501 cataaagaac tggtttagtc gtcaaaagga aaagaccttt gacaaccttt tgttcctcat
    10561 ctgtaaattc aatggtacga gctgatttcc catcttctag gactggttta atcttttgaa
    10621 gaacattgaa ttctgctact gattctttat ctttttgcgt acgtgccatc ttttctacac
    10681 gcgcatatcg tttgttcact gattctaagt cagcaagaat caattccaga ttaatggtat
    10741 caatatctgc aagcggatct acaaaggcgt cttcacgtcc ttgctcgcgc atcacatttt
    10801 catcatcaaa agcacgaact acgtgaacaa tcgcatctac ttcacgaata ttggccaaga
    10861 atttattccc tagcccctct ccttttgaag ctccttttac aatccctgca atatctgtaa
    10921 attcaaatgt tgtgggaact gtctttttag gagttatcat ttcagttagt ttttgtaggc
    10981 gttcatctgg aacttccacc attccaacat ttggatcaat cgtcgcaaat gggtagtttg
    11041 ctgcctctgc tcctgctttt gtaattgcat taaatagtgt tgatttacca acgtttggca
    11101 aaccaacgat acctgctgtc aaagccatat ttctcattct ccgttttcat ttcaatccct
    11161 aatattataa cacaaaaagg gataaagtgc taaccctcta actaagggtt ctcagtcaat
    11221 aattttattc atttttcgct caaaatcata tcgccccatc atgacaacat gctcacaatt
    11281 actgcatttt attttgatat ctgctcctac acgtgtaatt tcccaacgat tagccttttt
    11341 accagtcgac ttgattgtac aagcgtgtgg ttttttcatc tcaacaaaat ttccaacttg
    11401 atacatacta ctctcctttt ctttttcgat tatatcataa aagaggcgag ccaggctcaa
    11461 cctctttcac ttaatttgta cgaactggtg taatgagctg catgaagtct tcgtcagtat
    11521 ctgctggcac aagagtaaat ggacgaacag ctgagataaa gctaatggtc accttttcgc
    11581 tatttaaagc tttaagagaa tcaatcaagt aagttgggtt gaaactaatg gtcaaatctt
    11641 caccagtaac ctgatcagta tcgatttctt cgtttacttt accaacttct ggagagtgaa
    11701 catgggcgct aacaacccca tccttaattt caagtttcac agtaccattt tgagtcgcac
    11761 ttgataaaag acgggcacgc tccattgact ggcgtaagtt taccacatta aaagtaatag
    11821 tagtgttaaa gtctgttgga atcaagcgat ctgtatcagg atagtttcct tctaggagac
    11881 gagtatagaa gctaatattt tcgcttctaa agaggatttg gttattggca aagaaaatct
    11941 ctacagtttc gatatcatct gtaaataccg ctgaaaattc gcgtagagaa cggctaggaa
    12001 ttacgacatc aaaatcatca ctattttttt caagagtcaa ttttttctgg cttaggcgat
    12061 gagagtctgt tgcaactgtt tttaactctt tgtgttgact caatacgaag tggacacctg
    12121 ttaaaatcgg acgactctct tgtgtacttg cagcaaaggc tgtttcatta ataattttct
    12181 tgagtaattt tgtttcaagt attaaaggag tgcttgctga aatttcttgg attcgtggat
    12241 attgttcgct atcttttcct tttagggtaa tttctgattt gccactggtt aaaacaattt
    12301 gattttgttc aatttcttta aaatcaagag ttacatcagg taaactagat actacattga
    12361 taaagaaaga agcttcaaga aggatcgaac ctaaagaagt aatcaacaaa ccagcatctt
    12421 catttttttg agaaataaaa ttttcaattg agatttgacc atttgaacca attaaagtaa
    12481 taccttcatt ggtcacgtca atttttactg ttgataaaat aggaatggca tttttagaac
    12541 taatagctct cttagtagta tttaatgctt gtagaaataa atttttatta attgaaaaat
    12601 gaatcatgga ttctccttta tttattttta gtattagaaa gataatagta ataatagagt
    12661 ctgtgaaatc tgtggaaaac agcctttaaa caagtcctat caagtttttt agcttgttta
    12721 aaaaatgtgg ataaaaaaga taaaaaacta aaagttatcc acaaattatt tgattttctt
    12781 tttgattgat tcaatttcta aacgtaaatt atcgtcttga tcaatcaaag attttatttt
    12841 ggcatgggca tgaatgactg tggtatgatc ttttccccca aattccttcc caatttttgg
    12901 aagactatta tctgttagtt ctctagataa atacatggct acttgacggg ccaaaacaat
    12961 attttgaagg cgtctacttc ccttcatttc tttgatacta acaccataaa agttaccaac
    13021 ttcagtttgg attttatcaa ttgggatgac gagcatttgg ctaacatctt gtttgcgggc
    13081 tctaatggct tctgcagcaa tatcaatagt gatatccttg atttttttta ctctggcaat
    13141 taaagtgatg tcgttgatgg ctccctcaag atctcgaaca tttgaatcaa attgcccagc
    13201 taggtattct agagtatcac tttggaaatt gtagcctaaa tgttccgttt tactttgtaa
    13261 aatggcaata cgtgtttcaa agtcaggggg tgtgatagtt tgtgtcaatc cccaactaaa
    13321 acgcgtgaca agcctctcct cgagcccttc tagatgtttt ggactacgat cactcgttag
    13381 gacaatttgt ttttgcttgt catgaagggc gttaaaggta ttgaaaaatt cttcctgagt
    13441 tgcgactttt tttccgctga gtgactggat atcatcgatt aacaaaagat caagactacg
    13501 ataggtcttt ttaaactttt ccatttcccc aagtcttagg tgatcaagaa agtcattaat
    13561 aaagctttcg gcagggatat atttaacacg cgcattagga atatttttta gaatttcatt
    13621 tccaatagcg tttaataagt gagtcttacc aaggcctggt cctccataga taaaaagagg
    13681 gttataggtc agagccaaat cttcagagac agctaaagcg gctgatacag cccaaacatt
    13741 tccatcccct tgaataaagt tatcaaaggt atacttttct tttaatcccg tatctgaata
    13801 aggaatagat actaactttg gactatagtc ataaagagtt aaatttgtag cttcttcaac
    13861 ttgtgagcta gtcgtatctt gaggtttggt gaaaatatag tggggagtta tttcagcgtc
    13921 ataaatttca aaaccagcta ctacaataat atcttttagt tgtttttccc agaccatttc
    13981 catttcagag cgaggtagaa atatagtggc aacattttcc tctaccttga taagttcagc
    14041 ttgaatagca tagaaatcat acatggatcg agtcagtctt tcttgtgcaa attctaatat
    14101 acgattccaa aattgttttt ctttcaatcc tttctcctcc tttactattt aaaagtttaa
    14161 tactagagat attttaccat agatagcaag aattttccac aagctgtgaa aaataatcca
    14221 caatgttacc aaactttatc cacaggttgg ggataaaaga agaaattatt gatttattaa
    14281 gctttttatc gaaaaacata gtggataacc ttgtgagata aaaaaataaa agaacagcct
    14341 tatttcaggc tgttgataat tctactatat tcttcttgat ttgaaaaaga aataatgatt
    14401 tttccactgt cttttttaga tagtttaatt tctacatcta atccgagtag ttttcttaac
    14461 tgtttttctt cattttgtat gaaatgatta gttttttgct gtttcttttg ttttttctct
    14521 gtcagaagag cttctaattt ccttacagaa atatcttctt ctataatccg ttgaaagaaa
    14581 tagtcttgtt gttccttatt taacccaact agggaacgcg catgggcttg tgatagtttg
    14641 ccattttcta cttctgaaag aatctgttct ggcaaggaaa gtaaacgaat ggagttgctg
    14701 atatatggac gagacttgcc catcttatct gcaatttcag catgggtgaa tcctttctct
    14761 acgagagatt cataggcgcg tgcttcttct attgggttta aattttctct ctgtaaattt
    14821 tcaataatgg actggaccat catctcttgg tctgaaatct gtttaacaac agctgggata
    14881 gaccgtagac cagctaaaag tgaagcccga tagcgtctct ctcctgcaag gatttcataa
    14941 ccaataacag gagattgacg aacaataatc ggttgaatga ccccattttc tttgatagac
    15001 tgtgctagtt catctagttt ttctctatca aattcttttc ggggttgata gggatttttt
    15061 tgtatatctg tgatagaaat catttcaaat ttttccatga ttctacacta acacatcttt
    15121 tctcttatgt aaagctttct ttacatagat gtcaattaag attctaaatc acctgaactc
    15181 ttgttaagtt tgatagaggt agtttcttct ttcccgttac gatagtaggt tatcttaatg
    15241 gtgtctccga tagaatggtt gtaaagagca ctttgtaagt ctgttgatga agcaatctct
    15301 ttgtcatcta cttttgtaat tacatcgtat ttttcaaggt gaccattggc aggcatatta
    15361 ctttgtaccg aacgaacaat tacaccagat gtaacattac ttggaatatt gagtcttctg
    15421 atgtcgcttg tactcacatt agataaatta accatctgga ttcccaaagc tggacgcgtc
    15481 acttttccgt ttttttctaa ctgttcaata atattgatag catcatttgc aggaattgcg
    15541 aaaccaagac cttctacaga tgttcctcca tttgtagcaa ttttacttga ggtaattccg
    15601 ataacctgcc cttgaatatt gatcagtggg ccgccagagt tacctgggtt aatagcagta
    15661 tcagtttgga tggcttttgt agaaatagct tgtccatctt ccgattttaa ggatacattt
    15721 ctattgagac tggatacgat accttgagtg acagtatttg catattcaga acctaacggg
    15781 ctaccgatgg caatagcagt ttctcctaca gttaacttac tagaatcacc aaactcagct
    15841 actgttgtca ctttttctga agagattttg acgacagcaa tatcagagaa agtgtcagct
    15901 ccgacaattt ttccaggtac tttagtccca tctgacaatc gaatatctac tttgctggcg
    15961 ccatttataa cgtgattgtt ggtgacgatg taagcttctt tatcattctt tttataaata
    16021 actccagatc cttcactaga gattcgctga gaatctgtgt cagtatcatc attgccaaat
    16081 acgctatttt gtctgtttgc cgaataagta ataacagaaa caacagcatc ttttactttg
    16141 ttaacagcct gtgttgttga attttcgttc ttataggcag tttgtgtaat agtactattg
    16201 ttgttagagt tgtttacact acttttttga gttagttgag ttattgaaaa actacccaag
    16261 gctccactaa aaaagctaat gacgataacg actaataatt gaaaccattt tttgtaaaat
    16321 gtttttagat gtttcatatt tgcctccata tgtttgaatt actgaaagta taaactgact
    16381 agcttaatta taacttaaac acaaaagttt tacacaaact gtggataact cttttgaaat
    16441 tgtgattttc ttaattgaaa tctatttttt attttgtgaa taagatgtga aaaaatagag
    16501 aatatgttag aatagagtca tgaaaattaa agttgtaaca gttgggaaac tgaaagaaaa
    16561 gtatttaaaa gatggtatcg cagagtattc aaaacgaatt tctagatttg ctaagtttga
    16621 aatgattgag ttatcagatg aaaaaacacc agataaggcc agtgaatcag aaaatcaaaa
    16681 gattttagaa atagaaggtc agagaatttt atcaaaaatt gctgaccgtg atttcgttat
    16741 tgtgttagcc attgaaggga aaactttctt ctcagaagaa tttagtaagc agttagaaga
    16801 aacttctata aaaggatttt ctactcttac ttttattatt gggggaagtt taggattgtc
    16861 atcatctgta aaaaatagag ccaatctttc tgtcagtttt ggtcgcctaa ccttacccca
    16921 tcagttaatg agactagttc ttgttgaaca aatctatcgc gcttttacga ttcagcaggg
    16981 attcccctac cataaataga aaattgactt ttaattgaat ttttggtaga ataattgtat
    17041 taggtctcat agctcagctg gatagagcat tcgccttcta agcgaacggt cgcaggttcg
    17101 aatcctgctg ggatcaatat aatagcaaag ctgggaattt tcccggcttt tttcttaaaa
    17161 aagtacactt tgggagaaaa aaatgacagt tgagagaatt ttatctaaaa cgaaattcca
    17221 ttttgtataa tggtttttgt aagttagctt acaagaaaaa acattttagg agattttatt
    17281 atgaaaaaca cagttaaatt ggaacagttt gtagctttga aggaaaaaga cttacaaaag
    17341 attaaaggtg gggagatgag gttgtcaaaa ttcttccgtg attttatttt acaaagaaaa
    17401 aagtaatgaa ataaggggaa agagtaatgg atttatttgg atttgggacg gttattgttc
    17461 attttttaat tattagtcac agttaccatt ttatttgtaa aggtcaaata aatagaaaag
    17521 aattattcgt ttttggtgct tatacattac taacagaaat agtatttgat tttcccttat
    17581 atattctata tttagatggg ttagggattg aaagattttt atttcctttg ggattatatt
    17641 cctattttcg atggatgaaa cagtatgaga gggatagagg actattccta agtttactac
    17701 tatctctttt atatgagagc actcataact ttctgtccgt aactttctcc tctataacag
    17761 gagataattt tgttttacaa tatcatttcc cattcttttt cgttgtgacg gtgttaacct
    17821 attttgttac attaaaaatc atttactatt tccatttgga actagcctat tttgatgagg
    17881 actaccttta tcctttcttg aaaaaagtat tttttgcttt actattgcta catattgtat
    17941 ctttcgtttc agatatggta agtacgatta aacatttgaa tagttttgga agtattttgt
    18001 catctattgt ctttatctct ctccttttga ccttctttgc aatgaattct cataaagttc
    18061 aaatggagaa agagattgct ttgaagcaga agaaatttga acagaaacat ttacagaatt
    18121 acacagatga aattgttggt ctgtataatg aaatccgtgg ttttcgacat gattatgctg
    18181 gaatgcttgt cagcatgcag atggcaattg acagtggtaa tttacaggaa attgacagaa
    18241 tttacaatga agttttagtc aaagcaaatc ataaattgcg ttcagataag tacacttact
    18301 ttgatttgaa caacatagaa gactcagctt tacgaagttt ggttgctcag tcaattgtct
    18361 atgctcgaaa taatggtgta gagtttacac tggaagtaaa agatacgatt accaagcttc
    18421 caattgaact attggatttg gttcgtatca tgagcgtttt attgaataat gctgtcgaag
    18481 gatcggctga tagctataaa aagcagatgg aagtagcagt tattaagatg gaaactgaaa
    18541 cagttattgt gattcagaat tcatgtaaaa tgacgatgac tccttcagga gatctatttg
    18601 ccttaggatt ctccactaag ggaagaaatc gcggagtcgg attaaataat gtgaaagaac
    18661 tactagataa gtacaacaat attattttag aaacagagat ggaaggcagt acatttagac
    18721 aaatcattag atttaagagg gaatttgaat gaaagtttta attttagaag atgttattga
    18781 acatcaagtg agactagaga gaatattgga tgaaatttcg aaagaatcga atattccaat
    18841 atcatacaag acaacgggaa aagtccgtga atttgaagaa tacattgaaa atgatgaagt
    18901 aaatcagctt tatttcctag atatcgatat tcatggaatt gagaaaaagg gatttgaagt
    18961 ggctcagctc attcgtcatt acaatcctta cgctattatc gtctttatca ctagtcgatc
    19021 agagtttgcg actctaacat ataaatacca ggtatcagcc ctagattttg ttgataagga
    19081 tatcaatgat gagatgttta agaagagaat tgagcaaaat atcttctaca cgaagagtat
    19141 gttacttgaa aatgaagatg ttgtagatta tttcgactac aattacaagg gaaatgattt
    19201 aaaaattcct taccatgata ttttgtatat tgaaacaaca ggggtatctc ataaattgcg
    19261 cattattggt aagaattttg caaaagagtt ttatggtacc atgacagata ttcaggaaaa
    19321 ggacaaacat actcagcgat tttattctcc tcacaagtca tttttggtaa atataggcaa
    19381 tatcagagaa attgatcgaa aaaacttaga aattgttttc tatgaagacc atcgttgtcc
    19441 tatttcaaga ttaaaaatta gaaaattaaa agatatttta gagaaaaaat ctcaaaagtg
    19501 attgacaatt agcaagaaat tgatataatg gttatatctg ctacagatag aaggtccgtt
    19561 ggtcaagggg ttaagacacc gccttttcac ggcggtaaca cgggttcgaa tcccgtacgg
    19621 actattttat ccgccatagc tcagttggta gtagcgcatg actgttaatc atgatgtcgt
    19681 aggttcgagt cctactggcg gagtatagat aaaagggaac acagctgtgt tcctcttttt
    19741 gtatcaattt gtatcaccaa gcattttcat aaggaagtct gttatttctt gagaactttc
    19801 tttttttcca tgtgcaatcc aagtttggca gacaccaaaa agtgcatgag ttagatagat
    19861 gctactatat tctaattcgg tggtatttag attcagttgc ataaatcgct tttgtaaatc
    19921 tgtactaagc atgatatgaa gtttatttcg taagaaattt tggatttctt tagtcccatt
    19981 ttcagaaaga agggcagcca gaagtggttc tgactctaga tattcaaaaa cttctaaaat
    20041 agcgtctctt ttgtgatgag catgtttttg aaaaatatat tcaaatgtat ggaatagctt
    20101 gctttgatag tgctcaatca tatcatactt atccttatag tgagtataga agctggaacg
    20161 actaattccg gctttttcta ctaatttgac agtagaaatt ttatcaaatg gctgttccat
    20221 cagtaattgt accatagcat tttcaatagt tcgctttgtt tttaagcgtt tgttactttc
    20281 ttgcatattt cctccttgta aacaaattag actatatgtc taaaaataga ttttttatct
    20341 tgtaatttag attttttaat gtataatcta ttatatcaaa attttagaca atatgtttaa
    20401 aaaaggagaa actaagttta aagaatggaa agcaatttaa aaaaaaccaa cctttattat
    20461 tgtcatgatc gggatttctc ttattccaga tctgtacaat atcatatttt tgtcatcaat
    20521 gtgggatcca tatgggcaat tgtctgactt acctgtggca gttgtaaata atgataaaga
    20581 ggcttcctat aatggtaata ctatggcaat aggaaaagac atggtgtcca atttaaaaga
    20641 aaataaaacc ttggattttc attttgtaga tgaagaggaa ggaaagaagg gattggaaga
    20701 tggcgattac tatatggtag tgactttacc aagtgattta tctgaaaaaa caactacatt
    20761 atccaatatt caatcgacag cagcttatca atcattgaca agtgagcaac aaactgagat
    20821 aagtgattct gtatctcaaa attcaactga tagtattcaa tcggctcagt caattgtagc
    20881 tttagtacaa gatttacagg gaagtttaga aaacttacaa aatcaatctt ctaatctttc
    20941 gactttaaaa aatcaatcta atcaagtatc acctattact tctacttctt tgataggatt
    21001 gtcaagtgga ttaacagaga tacaaggaga tgttactagc aaattagttc ctgccagtca
    21061 gtcgattgca tcaggtgtaa acgcatatac tacaggtgtt gataaagttt ctcagggcgc
    21121 aagtcaacta agtgaaaaaa atgccacctt gacaggtagt ttggataaac tagtttcagg
    21181 ctcaaacacc ttgacacaaa aatcttctag attgacagca ggagttggtt aattacaatc
    21241 aggatctggg caattagcag acaaatccag tcagttactt tcaggtgctt ctccattaga
    21301 gaatagagct aataaattgg cagatggatc taggaaacta gcagaaggtg gaacaaagtt
    21361 aacttctgga ttggaagatt tacagacagg acttgcttct ttaggacaag gactaggtaa
    21421 tgctagtgat caactcaaat cagtatcaac agaatctaaa aatgcagaga ttttgtcaaa
    21481 tccactcaat ctttcaaaaa cagacaatga tcaagttcct gtaaatggaa tcgcaatagc
    21541 tccttatatg atatcagttg ctcttttttt tgcagcaata tcaacaaata tgatatttgc
    21601 gaaattgcct tcaggacgtc atccagagag ccgttgggct tggttgaaat cttgagctga
    21661 aataaatggt attatagctg ttttggcagg aattttggta tatggaggag ttcagcttat
    21721 tggtttaact gctaatcatg agatgagaat atttattctc atcatcctaa caagtttagt
    21781 attcatgtct atggtgacca ctttagcaac gtggaatagc cgtataggag cttttttctc
    21841 acttattttg cttttactac agttagcatc aagtgcaggt acttatccac ttgctttgac
    21901 aaatgatttc tttagatcta ttaatccctg gttaccaatg agctattcag tttcgggatt
    21961 acgacaaaca atctctatca acaagtcatt ttcctagctg tcatactagc tctatttact
    22021 ggtttaggta tgctagccta tcaacataag aaaatggaag aagattaaaa aaatcgaccg
    22081 attaactggt cgatttttta tgccttagat gactttcgtc tgtgattata gattccaaat
    22141 agtaagagag aagtaaagga acagattgct ccagtaataa aaccattggg aatgaaggaa
    22201 agtgtaatag ttcctttccc cttgggaatg tcaactttca taaatccagt ttgagcttgt
    22261 ttaatttcta ttttcttacc atcttggtag gcagaccaac ctttgtcata aggaatggtg
    22321 aagaaaatag atgtatcttg ttggacatca tatgtagcaa aaaccttgtt tttagaagtt
    22381 gatactgtga caggttgttc tttaattttt tgaattgcct cggtgaaagt tttggtatct
    22441 aaacgataga aggtaggaga ttcaaatgat acttgtgaat ttccagggaa actaacattg
    22501 atattgaaag tttttttctc tttagtatat cctagattaa agaaggagaa gacattatca
    22561 gttgtaaaag tctttttttc accatttaca aggatgtcaa ccttcttttg tttatcgtta
    22621 gaaaagtgaa ggtttatgaa agagagataa acttggctgt tttctggaac ttcaatttga
    22681 tactggattg ctgcatcttc atttgaagaa cttgtgacac taatcaaatc attagtattt
    22741 tctatttttt ctgttttttc ataaggtatt ggagaaaaat aatcaaaatt gacgttagca
    22801 agttgattta aaaatgaggc ctgattatcc aaggtatgtt cattgaactt gacatcattg
    22861 taaacagatt gactagcaac tgcaatcgga agagagtatt gattttcata tagggtaaga
    22921 ttatcttttt gatagatatc tttaaagcca tacttatcaa taggactgtc tgagatattg
    22981 tactggatac caaataaact atcagccaaa atactattat ttgcatatcg gagattgaga
    23041 ttagtcccag aggatttaaa accaagttta tctaaagtag agcttgatga acgatttcga
    23101 acagatgaaa attgagagat tccattgtag ttgaatttca tactgtcatt tcctgtctga
    23161 gtttgtagtt tttcagtacg agtaaattga tttccaatat atgttgagaa agattccata
    23221 gctaggatat ctcgactata agcacttcga gaagcaaatc cccattcctt agcaattccg
    23281 tccatttgag atgaagcatt taaactcatt tcaaccagta taaataaaga gattagaatg
    23341 gcaaatagat tcacagatat aaactttttg ataactgcaa ggagtaaaag agaatagaca
    23401 accaaaaatt caagagtaag cagaatattc aaatctgtta aaaaagaata atgcgatttt
    23461 agatagatgg tagctaaaaa tcctgctact acaagaaaaa gcgaaactaa aaaattccag
    23521 actttaagtt ctttcagacg ctttaagact tctgctgctg tgtaaattaa caaggtagag
    23581 aaaatccaag catagcgatg taaaaacatg tttggagtat gcatgccttg ccaaaataag
    23641 tcaagagctt ctatgtaaaa gcttgcaatt agaaatgcaa agaatattac atatatgagt
    23701 ttcacgtgaa acttaataga tttcagcgta aaaaataaaa tggtcaaaat aaagggaaat
    23761 agtccaacaa aaatcattgg gatggcccca tactttgttg tgtcaaagga accaatgaat
    23821 tgcttagcaa agagatcaag ataccagcta ctttcagttt gaaactttgt aacttcagtc
    23881 aatttttccc catgtgtctg taaatcaaat agagtgggaa gagtcataat caaactagcc
    23941 ataccagcta aaaaggagat aactatgaaa tcaagaacag atgattttcg agtcttaaag
    24001 tcccacgaaa tttgacagag ataccagaaa ataagaaaca atactgtcat atatccaaaa
    24061 taataatttt gaataaataa gattgacaga cttgtaaagt acaataggag tttcttttca
    24121 gttatcagta gatgtaaacc agttataatt aaaggaatca agataaaaac atctagccag
    24181 gtttttatct ctaattgact gacagtgaaa ctcatcagag cataggaagt agataaggct
    24241 agttttaaag tctgagggat agattgaaac aatttattca aactaaaaaa ggttgacaga
    24301 ccaatcaatc caaattttaa gagagtggtc agatagacag catctggcat gttcgttaga
    24361 tcaaaaaagt aaactagagg cgcgagaaaa ctacccaagt aataactaga tagggcatag
    24421 aagtttagcc ctagaccact tgtaaaggtg taaaacagac tactatttcc atgtaggata
    24481 tttcgtaagg ctacatcaaa aataacgtat tgatgaaagc catctcctaa tagaggagag
    24541 ttgtcgctat tccagtagat accttgagat agatatactc cagacataat cactacagga
    24601 atgatgagag aaataaaata ggttcgatat gtttttaaaa atgatttcat gttacctcgt
    24661 agaatgatag aaaactcagt tggttaaccc aactgagttt tgaagtttta tttagtcttt
    24721 ccaaagttct ttaacttttg cttgtacttc tgcattttct aggaattcat cgtaggtttc
    24781 atcgatacgg tcaatgacgc catttttaga taagacaatg atatggttag ccaaagtttg
    24841 aataaattcg tggtcatggc tagcaaagat gattgattct ttaaagtttt tcaatccatc
    24901 attcaagctt gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa gtacaaggac
    24961 atttgatttt aagagcatga gttttgaaag catgacacga actttttctc cccctgacaa
    25021 gacatttaca ggtttgttaa cttcatctcc agagaagagc atacggccga ggaagccacg
    25081 taggaaagta ttgtcatctt cttctttact tgcgaattga cgcaaccagt caagaattga
    25141 ttctcctcct gcaaaatcag ctgagttatc ttttggtagg taagattgac tagttgtaac
    25201 tccccacttg acagttcctt catagtcaat atctcccatg attgcacgaa ttaatgcagt
    25261 cgtttgaata tcattttgtc caataagtgc tgtcttatca tctggacgca agatgaaact
    25321 aatattatcc aagatagttt caccatcaat ctttacagtt agattttcta ctgtcaagag
    25381 atcattacca atctcacgtt ccgctttaaa gttgataaat ggatatttac gactagatgg
    25441 tacaatctct tctagctcaa tcttatcaag cattttctta cgtgatgttg cctgccttga
    25501 cttagaagca ttggcagaga aacgagcaac gaattcttgc aattgtttaa ttttttcttc
    25561 tgctttagca ttacggtctg ctagcaattt agcagcaagc tcagaagatt ccttccagaa
    25621 gtcgtagttt ccgacataga gtttgatttt tccaaagtca aggtcggcca tgtgagtaca
    25681 aactttgttt aagaagtgac ggtcgtggga tactacgata actgtgttat caaagtcaat
    25741 caagaagtct tctaaccaag taatcgattg gatatccaaa ccgttagtag gctcgtccaa
    25801 gagaagaaca tctggtttac caaaaagtgc tttggcgagg agaaccttta ctttttcacc
    25861 gttggccaat tcgctcatgt tttggtagtg taattcttct ggaatgttta ggttttgaag
    25921 tagttgagag gcttcactct ctgcttccca acctccaagt tcggcaaact ctccttcgag
    25981 ttcggcagca cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga tagcatcttt
    26041 ctctttcatg atgctataaa gtttttcatt tcccatgata acgacatcaa tggcacgttc
    26101 atcttcgtag tcaaagtgat tttgacgaag aacagagaga cgttcatctg gaccaagaga
    26161 gatgtgacca gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg ttgattttcc
    26221 ggcaccatta gcaccgatta atccgtaagt atttccttct gtaaatttga tattgacatc
    26281 atcaaaaagt ttgcgatcac taaaacgtag tgaaacatca gatactgtaa gcaatatttt
    26341 tctcctatat gtgtaatata tttattctac tagaaaatac agaaatattc aaatttttat
    26401 ttgtcaattt tgtgtaaatt atatttacag tatcctttac acaaatctgt aaaaagcaag
    26461 gctgatttat tttgataaat tacggttatt tcattaaaaa aatgctataa ttgaaaggac
    26521 tatatcgaag gagaacaaaa tgactaaacc cattatttta acaggagacc gtccaacagg
    26581 aaaattgcat attggacatt atgttggaag tctcaaaaat cgagtattat tacaggaaga
    26641 ggataagtat gatatgtttg tgttcttggc tgaccaacaa gccttgacag atcatgccaa
    26701 agatcctcaa accattgtag agtctatcgg aaatgtggct ttggattatc ttgcagttgg
    26761 attggatcca aataagtcaa ctatttttat tcaaagccag attccagagt tggctgagtt
    26821 gtctatgtat tatatgaatc tagtttcgtt agcacgtttg gagcgaaatc caacagtcaa
    26881 gacagagatt tctcagaaag gatttggaga aagcattccg acaggattct tggtctatcc
    26941 aatcgctcaa gcagctgata tcacagcttt caaggctaat tatgttcctg ttgggacaga
    27001 tcagaaacca atgattgagc aaactcgtga aattgttcgt tcttttaaca atgcatataa
    27061 ctgtgatgtc ttggtagagc cggaaggtat ttatccagaa aatgagagag cagggcgttt
    27121 gcctggttta gatggaaatg ctaaaatgtc taaatcacta aataatggta tttatttagc
    27181 tgatgatgcg gatactttgc gtaaaaaagt aatgagtatg tatacagatc cagatcatat
    27241 ccgcgttgag gatccaggta agattgaggg aaatatggtt ttccattatc tagatgtttt
    27301 tggtcgtcca gaagatgctc aagaaattgc tgatatgaaa gaacgttatc aacgaggtgg
    27361 tcttggtgat gtgaagacca agcgttatct acttgaaata ttagaacgtg aactgggtcc
    27421 tattcgtgag cgccgtattg aatttgctaa ggatatggga gaagtttata atatgattca
    27481 aaaaggtagt gaaagagcgc gtgaagttgc gggtcaaacc ctatctgagg taaaaggggc
    27541 aatgggactt cattacttta actaagttta ttttacaaga aactatcatt tctatctcga
    27601 agaaaagata gttttttatt tacccctgta acatttgaca aatttttata gaatggtatg
    27661 atagatacaa tataaaaaga gtcaagctca aaaagaaaga aaagaggaaa cttcgaatgt
    27721 ctaattggga cactaaattt ttgaaaaaag gttttacctt tgatgatgta ttgcttattc
    27781 cagctgaaag tcatgtgttg cctaacgatg cagatttaac aactaaattg gcagataatt
    27841 tgactttaaa tatcccaatt attaccgctg ccatggacac agttacagag agtcaaatgg
    27901 ccattgctat tgctcgtgca ggtggtctcg gagttatcca taaaaacatg tcaattgctc
    27961 aacaagcaga cgaggttcgt aaggtaaaac gttctgaaaa tggagttatt attgatccgt
    28021 tcttcttgac gcctgaacat acaattgctg aagcagatga gcttatgggt cgttaccgca
    28081 tcagtggtgt tccagttgtt gaaacacttg aaaatcgtaa attggttggt attttgacaa
    28141 accgagatct tcgttttatt tcagattata atcaaccaat ttcaaaccat atgactagtg
    28201 aaaatcttgt tactgctcct gtgggtacgg atcttgcaac ggctgagagt attcttcaag
    28261 agcatcgtat tgaaaaactt ccgttggtcg atgaagaagg cagtctttct ggtttgatca
    28321 ctatcaaaga tattgaaaaa gttattgagt ttccaaatgc ggctaaagat gaatttggtc
    28381 gtcttctagt tgcaggtgca gtaggtgtta cttcagatac atttgaacgt gcagaggctc
    28441 tttttgaggc aggagcggat gcgattgtta ttgatactgc acatggtcat tctgcaggtg
    28501 tcttgcgtaa aattgccgag attcgtgctc atttcccaga tcggactttg attgctggaa
    28561 atattgctac tgctgaaggt gcacgtgccc tttatgaagc gggtgtagac gttgttaagg
    28621 ttggtattgg accaggttct atctgtacta ctcgtgtgat tgctggtgtt ggtgttccgc
    28681 aagtaacagc tatctacgat gctgcagctg ttgcgcgcga atatggtaaa acgattattg
    28741 ctgacggtgg gatcaagtat tctggagata ttgtaaaagc acttgctgca ggtggaaatg
    28801 ctgttatgct tggatctatg tttgctggaa ctgatgaagc tccaggcgaa actgaaatct
    28861 tccaaggacg taaattcaag acttaccgtg gtatgggatc aattgctgct atgaagaaag
    28921 gttcaagcga ccgttatttc caaggttctg tcaatgaagc aaacaagctc gttccagaag
    28981 gaattgaagg tcgtgttgct tataaaggag cggcagctga tattgttttc caaatgattg
    29041 gtggtattcg ctctggtatg ggttactgtg gtgcagctaa ccttaaagaa ctacacgata
    29101 atgctcaatt tattgaaatg tctggtgctg gtttgaaaga aagccatcct catgatgtgc
    29161 aaattactaa tgaggcacca aattattcta tgtaaaaaac aatgaaaaga actccagtga
    29221 aaacaggagt tcttttacaa tgttgtcaat ttccatttac agaagcttta ccatcctgaa
    29281 tagtgaagat acttagattt tctggcagat tttgaagatg gtctaagctt gttgttgtga
    29341 taaaggtttg gattgattga gaaatcgttt ctaataattt taactgtcta gtgttgtcaa
    29401 gttcactcat cacatcgtca agcagtaata taggagattc tgtggtaatg ctttccatta
    29461 attcgatttc tgctaatttt atcgagagga cgagactacg atgttgacct tggcttccga
    29521 aactagcatc catcccattt atataaaaag aaatgtcatc tcgatgagga ccgacaccag
    29581 tattcttttt aaataaatct ctggatctac ttttttctaa agcaattttg aaagattcgg
    29641 ataagttttg tttgtcagtt atattgacag aagattgata ggatattgac aactcttcga
    29701 tctgattaga gagttcaaaa tgtttcttac gcccaaatga ttctagtttt tttatgaaat
    29761 ctaagcggtg attcattaca cgacatccat aatcaactag ctgatcatct aacacagaaa
    29821 ggaatgtttc atctattttt tgagctgatt ttaggtaagt gtttctttgc tttaggatgt
    29881 ggttataatt ggttaagtca gataaataga ttggcttaat ttgcccaagt tccatatcaa
    29941 tgaattttcg tcgaatcgaa ggtgctcctt taattagttg taaatcttca ggagcaaata
    30001 agacaacatt catgtgtcct acataatctg aaaggcgtgc ctgttttaag tgattaactt
    30061 ttgttacacg gcctttttgt gttagttcga tttctagggg aatggatcca gtttttttct
    30121 gaacgagacc tgaaagatga agttgttcct catcaaaatg aatgagattt ttatctgttc
    30181 gagttcgatg actacgcgtt aaggctaaaa aatagatagc ctctaacatg tttgtttttc
    30241 cttgtgcatt acgtcccaaa aagacattca atttaggatt aaagtctatt ttcgtttctt
    30301 tgtaattacg aaaagtcttg agagatagat gttgtagcca catgattatc taccagggaa
    30361 tcgtggagct tgttggcttt taggtgatga agcaggtttc gatttgtctt ttttaactcc
    30421 cttattcatc tttttgacaa gtttagcgat ccgttctttt tcaactttat cagcttggta
    30481 ttcatcttgt tcttcagaag taggttgtgt caacaagatg tcaatgttca tgtcagggat
    30541 gtcaacttta tcgccaatac gaagtttttt tccacgacga ctctctaatt ccctattaaa
    30601 gtaaacagaa tgttcagaga gaaatgattt aatagctcct ccgctatgtg taattccaag
    30661 ttctttgagt agtgcttgga gggtaataaa ttcttcaaat aatttgtatt ccataattca
    30721 cctcaatctt tggtattata ccatattttc ctaaaaatag tgagagagac agcgagaaat
    30781 gtaagcgatt tttatttcaa aagtgttaca gggaacaaga aaatttcagg ttttcgtgat
    30841 ataatagaag tctgtatata aggaggtaaa tcatggagtt agtgcatgga atttcaacac
    30901 attttatcca atcaaaaaag tttaaaacaa acaaaattac cgtgcgtttt accgctccat
    30961 tatcccttga tacgattgca ggtcacatgt tgagtgcaag tatgctagag actgctaatc
    31021 agatgtaccc cacttctcaa gatttgagga gacacttggc cagtctatac ggtacagata
    31081 tgtcaaccaa ttgtttcaga agagggcaaa gccacattat agaattgaca tttacctatg
    31141 ttcgtgatga gtttttaagt aggaaaaacg tgctaacctc tcagattttg gaacttgtaa
    31201 aagaaactct tttttcaccc gcagtagttg ataatgggtt tgatccggcc ttatttgaaa
    31261 ttgagaaaaa acaattgcta gcaagtttag cagctgatat ggatgattct ttttattttg
    31321 cacataaaga attggataaa ttgttttttc atgatgaacg tcttcaattg gaatatagtg
    31381 atttacgaaa tcgtatttta gctgaaactc cacaaagttc ttattcttgt ttccaagaat
    31441 ttttagccaa tgatcgaata gatttctttt tcctaggtga ttttaatgag gttgaaattc
    31501 aaaatgtatt agaatcattt ggctttaaag gtcgaaaagg agatgtgaag gttcagtatt
    31561 gtcaacctta ttctaatatc cttcaggaag gtatggttcg gaaaaatgtg ggacaatcca
    31621 ttttggaatt aggttatcat taccgttcta aatatggtga tgagcaacat ttacccatga
    31681 ttgtaatgaa tggtttactt ggtggatttg ctcactctaa gctctttaca aatgtccgtg
    31741 aaaatgctgg attagcttat accatttcaa gtgagcttga tttatttagt ggattcttga
    31801 ggatgtatgc tggtatcaat cgagaaaatc gtaaccaggc tcgtaaaatg atgaataatc
    31861 aactgcttga tttaaaaaaa ggttatttta cagagtttga gttaaatcag accaaggaaa
    31921 tgattcgttg gtcgttgtta ctttctcaag ataatcaatc ttcattgatt gaacgtgctt
    31981 atcaaaatgc cttatttgga aaatcttcag cagactttaa aagttggatt gcagagcttg
    32041 aacaaattga caaagatgct atttgtagag tagctaataa tgtgaaacta caagcgattt
    32101 actttatgga aggaatagaa tgacaaaggt tgtttttgaa gaaaaatact atccagctgt
    32161 aaaagaaaag gtttatcgaa ctcgtttggc caacggattg acagttgctc ttttgcctaa
    32221 aaaggaattt aaagaggttt acgggagtgt cactgtacag tttggttcgg tagatacgtt
    32281 tgtcacagaa gttgacggat atgtaaaaca atatcctgga ggaattgctc attttcttga
    32341 acataaatta tttgagagag aagattctag tgatttgatg tcggctttta cgagtctagg
    32401 tgcagatagt aatgccttta caagctttac aaaaacaaac tatctttttt cagcaacgga
    32461 ttatttttta gaaaatttag atttacttga tgaattggta acatcagcac actttactga
    32521 agcttccatt ctgacagagc aggatattat tcagcaagaa cgagaaatgt accaagatga
    32581 tccagattcg tgtttattct tttcaacttt agcgaatttg tatcctggta cacctttagc
    32641 aactgatata gttggaagtg aggagtccat ttcccaaatc aatctaacta atttgcaaga
    32701 aaattttaca aagttttaca aacctgtaaa catgtctctg tttttagttg gtaattttga
    32761 tgtggagcga gtacaggact attttgaaag caaagaactg aaagattcag attttcagga
    32821 agtagcaaga gaaaagttgt ttttacagcc tgtaaagcca acagatagta tgagaatgga
    32881 agtatcttct cccaaactag cgattggagt tagaggtaag cgagaagttt ctgaagcgga
    32941 ttgctatcga catcatattt tattaaaatt attgtttgca atgatgtttg gttggacttc
    33001 ggatcgtttt caaaaatgtt atgaatcagg taaaattgat gcgtccttat ctctggaagt
    33061 tgaaataaca agtcgctttc attttgtcat gttgacaata gatacgaaag agccagttgc
    33121 tttgtctcat caatttagga aggctattcg taattttaca aaggatttag atattacaga
    33181 ggaacattta gatattatca aaagagagat gtttggcgaa tttttcagta gcatgaactc
    33241 tcttgaattt attgcaacgc aatatgatgc ttttgaaaat ggtgagacaa tttttgattt
    33301 gccgaaaatt ttacaggaaa ttactttaga ggatgtcctt gatgctggac atcatttaat
    33361 agatgatggt gacatagttg attttacaat attcccatcg tagtaaccta tcataataga
    33421 cactagaaag aagggatgac aagtatgaga aaaaaaacaa ttggagaggt tttacgatta
    33481 gctagaatca atcagggatt gagtttagat gaattgcaga aaaagacaga aatccagtta
    33541 gatatgttgg aagcaatgga agcagacgat ttcgatcaac ttccaagtcc tttttacacg
    33601 cgttctttct tgaaaaaata tgcatgggct gttgagttag atgaccaaat tgttttggat
    33661 gcttatgatt ctgggagtat gattacttat gaggaagtag atgttgatga agatgagttg
    33721 acaggtcgta gacgttcaag taagaaaaag aagaaaaaaa catcattttt acctttattt
    33781 tattttatcc tttttgcttt atcgatttta atttttgtga cttattatgt ttggaactat
    33841 attcaaactc aaccagagga gccttctctt tctaattaca gtgtggttca atcaacaagt
    33901 tcaactagct ctgttcccca ctcctcaagt agtagttctt ctagtataga atcagctata
    33961 agtgtatcag gcgaaggaaa tcatgtagaa atcgcttata agacaagtaa ggaaacagtt
    34021 aaattgcaat tggcagtttc agatgttaca agttgggtca gtgtttcaga aagcgaactt
    34081 gagggcggtg taaccttatc gccaaagaag aaaagtgcag aagcaacagt tgcaactaaa
    34141 agtcctgtaa caattacgtt aggtgttgta aaaggtgttg atttgacagt agataatcag
    34201 actgttgatt tatcgaaatt aacagctcag actggacaaa tcactgtaac ctttactaaa
    34261 aattaaggaa aaacgaatga aaaaagaaca aattcccaat ctcttaacaa taggtcgaat
    34321 tctctttata cctattttta tctttatttt aacgatagga aattcgatag agagtcatat
    34381 agttgcagct attatctttg ctgttgccag tattaccgac tatttagatg gatatttagc
    34441 tcgtaaatgg aatgtggtca gtaattttgg taaatttgca gatcctatgg cggataagtt
    34501 actagttatg tcggctttta ttatgttgat tgagttaggt atggctccgg cttggattgt
    34561 tgcagtgatt atctgtcgtg agttagctgt gacaggttta aggcttttat tggttgaaac
    34621 tggtggaaca attttagcag cagcaatgcc tggaaaaatt aaaactttta gtcagatgtt
    34681 tgctattatt ttcttgctat tacattggac tttgcttggt caagttctac tttatgtagc
    34741 cttatttttc actatctact ctggctatga ctatttcaag ggtagtgcct atgtatttaa
    34801 agggacattt ggttcgaaat gaaatcaata attgatgtaa aaaatctttc ttttcgctat
    34861 aaagaaaatc agaactacta cgatgtgaag gatattacgt ttcacgtgaa acgtggagaa
    34921 tggctttcga ttgtagggca taatggtagt ggtaaatcaa cgacggttcg attaattgat
    34981 ggcttactgg aagcagaatc cggagagatt gtaattgatg gccaacgttt gactgaggaa
    35041 aatgtttgga atatacgtcg tcaaatcggt atggtttttc aaaatccaga caatcaattt
    35101 gttggagcga ctgttgaaga tgatgttgct tttggtttgg aaaatcaggg actttctcgt
    35161 caagaaatga aaaagagagt ggaagaagct ctggctttag ttggcatgtt ggactttaaa
    35221 aagagagagc cagcgcgtct atcaggtggc caaaagcaac gtgtggccat tgcaggtgtt
    35281 gtagccctaa gaccagctat tttaatctta gatgaagcaa cgagtatgtt ggatcctgag
    35341 gggcgtagag aacttattgg gacagtaaaa ggaattcgaa aagactatga tatgacggtc
    35401 atttctatta cccatgattt ggaagaagtc gccatgagtg atcgcgtatt ggtcatgaaa
    35461 aaaggggaaa ttgaatcaac tagtagtcca agggagcttt tctctcgaaa tgatttagat
    35521 caaattggat tagacgatcc ttttgctaat caattaaaaa aatctttgag ccagaatggc
    35581 tatgatttac ctgaaaatta tttgacagaa agtgagctag aggataagct atgggaattg
    35641 ctctagaaaa tgtgaatttt atatatcaag aaggtactcc cttagcttca gcagctttgt
    35701 cggatgtttc tttgacgatt gaagatggct cttatacagc tttaattggg cacacaggta
    35761 gtggtaaatc aactatttta caactcttaa atggtttatt ggtgccaagt caagggagtg
    35821 tgagggtttt tgatacctta atcacctcga cttctaaaaa taaagatatt cgtcaaatta
    35881 gaaaacaggt tggcttggta tttcagtttg ctgaaaatca gatttttgaa gaaacggttt
    35941 tgaaggacgt tgcttttgga ccgcaaaatt ttggagtttc tgaagaagat gctgtgaaga
    36001 ctgcgcgtga gaaactggct ctggttggaa ttgatgaatc actttttgat cgtagtccgt
    36061 ttgagctgtc agggggacaa atgagacgtg ttgccattgc aggcatactt gccatggagc
    36121 catctatatt agtcttagat gagccaacag ctggtctaga tcctctaggg agaaaagagt
    36181 tgatgaccct gttcaaaaaa ctccaccagt cagggatgac catcgtcttg gtaacgcatt
    36241 tgatggatga tgttgctgaa tatgctaatc aagtctatgt aatggaaaag ggacgtttag
    36301 taaagggggg caaaccaagt gatgtctttc aagacgttgt ttttatggaa gaagttcagt
    36361 tgggagtacc taaaattacg gccttttgta aacgattggc tgatagaggc gtgtcattta
    36421 aacgattacc gattaagata gaggagttca aggagtcgct aaatggatag tatgattttg
    36481 gggcgttata tcccagggga ttcgattgtt caccgattgg atccacgtag caaattgctt
    36541 gctatgatgc tactgatttt gattgtgttt tgggctaata accccttgac gaatctgatt
    36601 ctttttatag cgacagggat atttattgcc ttgtcaggag tatctctttc attttttatt
    36661 cagggcttga agtctatgtt tttcttgatt gccttcacaa ctatttttca actatttttc
    36721 atttctaatg ggaatgtttt atttgagttt tcgtttgtga gaatcacgga ttatgctttg
    36781 caacaagctg ggattatttt ttgtcgcttt gtattgatta ttttcttttc aactttgtta
    36841 accttaacaa ccatgccttt aagtttggca tcagctgtcg aagctttatt agcaccttta
    36901 aagcgtgtga aagttccagt tcatgaaatt ggattgatgc tgtctatgag tttgcgtttt
    36961 gtcccaacct tgatggatga tacgactcgg attatgaatg cacagaaagc tcgtggagtg
    37021 gattttggag aaggaagcat cgttcaaaaa gtaaaggcga tgattcccat tttgattcct
    37081 ctttttgcga caagtttaaa acgtgcagat tccttggcta tcgctatgga agcgcgtggc
    37141 tatcagggtg gaaaaggcag aagtcaatac cgacaattga aatggactct aaaggatacg
    37201 ctgaccattc ttgttattct cgtacttggt tgttgtttat ttttcttaaa atcttagtag
    37261 ctttcaggaa ttgataaaaa gttactgtaa cagtttttga tataaaggta gggatatgaa
    37321 ccgttttaaa aaatcaaaat atgtcattat tgtttttgtc actgttctgc ttgtgtcagc
    37381 tctcttagcg acgacttatt caagtacaat tgtgacaaaa ttaggagatg gaatctcatt
    37441 ggttgataga gttgtacaaa aaccttttca gtggtttgat tctgtcaaat cagatttggc
    37501 tcatttgaca cgaacatata atgaaaatga aagtttgaag aaacagcttt accaattaga
    37561 agttaaatca aatgaggtgg aaagtttaaa gacagaaaat gaacaactgc gccaattgct
    37621 tgatatgaag tctaaattgc aagccacaaa gactttagca gcagatgtta ttatgcgttc
    37681 tccggtatct tggaagcagg agttgacctt agatgcaggt agatcaaaag gtgcttctga
    37741 gaacatgtta gctattgcaa atggtggctt gattgggagt gtttcaaaag tagaggagaa
    37801 ctctactata gtcaaccttc tgacaaatac ggaaaatgct gataagattt ctgttaaaat
    37861 tcaacatggc tctactacaa tttatggaat tattattggc tatgacaagg aaaatgacgt
    37921 tcttaaaatt agccaattaa atagtaatag cgatattagt gcgggagata aggtgactac
    37981 tggtggatta ggaaacttta acgttgctga tattcctgtt ggtgaagtgg ttgccacaac
    38041 gcatagtaca gactatttga cacgagaagt aactgttaaa ttgagtgcag atactcataa
    38101 tgtagatgtg atagaattag tggggaattc ataatgagac agttgaagcg agttggagta
    38161 tttttattgc ttcctttctt tgttctaatt gacgcccata ttagccagct tctgggctca
    38221 tttttccccc atgtacattt ggctagtcat tttctttttc tatttctctt atttgagacg
    38281 atagaagtat cagagtatct ctacctagtc tattgttttg ttataggctt ggtttatgat
    38341 gtttactttt tccatctaat agggattaca actctcttat ttatcttatt gggagccttc
    38401 cttcataaat tgaatagtgt tattttgttg aatcgttgga caagaatgct agctatgatt
    38461 gtgctgacat tcctgtttga aatgggtagt tatcttttgg cttttatggt agggttgaca
    38521 gtagatagca tgtcgatttt tatagtctat agcttggtac cgacgatgat tttaaatttt
    38581 ttatggatta ctgtttttca atttattttt gaaaaatatt atctataaga acgacatata
    38641 aatgtaacaa aggcgtaata tttattaggc ctttttttgg tatactagta ttgtctttaa
    38701 aagaaggagt atctacgtaa tatgaagaaa aaaatcttag cgtcactttt attaagtaca
    38761 gtaatggttt ctcaagtagc tgttttaaca actgcgcatg cagaaacgac tgatgacaaa
    38821 attgctgctc aagataataa aattagtaac ttaacagcac aacaacaaga agcccaaaaa
    38881 caagttgacc aaattcagga gcaagtatca gctattcaag ctgagcagtc taacttgcaa
    38941 gctgaaaatg atagattaca agcagaatct aagaaactcg agggtgagat tacagaactt
    39001 tctaaaaaca ttgtttctcg taaccaatcg ttggaaaaac aagctcgtag tgctcaaaca
    39061 aatggagccg taactagcta tatcaatacc attgtaaact caaaatcaat tacagaagct
    39121 atttcacgtg ttgctgcaat gagtgaaatc gtatctgcaa acaacaaaat gttagaacaa
    39181 caaaaggcag ataaaaaagc tatttctgaa aaacaagtag caaataatga tgctatcaat
    39241 actgtaattg ctaatcaaca aaaattggct gatgatgctc aagcattgac tacgaaacag
    39301 gcagaactaa aagctgctga attaagtctt gctgctgaga aagcgacagc tgaaggggaa
    39361 aaagcaagtc tattagagca aaaagcagca gctgaggcag aggctcgtgc agctgcggta
    39421 gcagaagcag cttataaaga aaaacgagct agccaacaac aatcagtact tgcttcagca
    39481 aacactaact taacagctca agtgcaagca gtatctgaat ctgcagcagc acctgtccgt
    39541 gcaaaagttc gtccaacata cagtacaaac gcttcaagtt atccaattgg agaatgtaca
    39601 tggggagtaa aaacattggc accttgggct ggagactact ggggtaatgg agcacagtgg
    39661 gctacaagtg cagcagcagc aggtttccgt acaggttcaa cacctcaagt tggagcaatt
    39721 gcatgttgga atgatggtgg atatggtcac gtagcggttg ttacagctgt tgaatcaaca
    39781 acacgtatcc aagtatcaga atcaaattat gcaggtaatc gtacaattgg aaatcaccgt
    39841 ggatggttca atccaacaac aacttctgaa ggttttgtta catatattta tgcagattaa
    39901 tttacagagg gactcgaata gagccctctt ttcaggtttt accgtgacaa tccctattaa
    39961 aaattatatc aaaatagctt gaaaatattg gaaaagtatg gtagaatgaa aattgtcgtg
    40021 tgaacgataa tactcattct tgatgaattg tgaagcagtt gcccttgggt cgttttgcga
    40081 gttgaagtca agaagaggaa aaaaacaaaa aggagaaata ctcatggcag taatttcaat
    40141 gaaacaactt cttgaggctg gtgtacactt tggtcaccaa actcgtcgct ggaatcctaa
    40201 gatggctaag tacatcttta ctgaacgtaa cggaatccac gttatcgact tgcaacaaac
    40261 tgtaaaatac gctgaccaag catacgactt catgcgtgat gcagcagcta acgatgcagt
    40321 tgtattgttc gttggtacta agaaacaagc agctgatgca gttgctgaag aagcagtacg
    40381 ttcaggtcaa tacttcatca accaccgttg gttgggtgga actcttacaa actggggaac
    40441 aatccaaaaa cgtatcgctc gtttgaaaga aattaaacgt atggaagaag atggaacttt
    40501 cgaagttctt cctaagaaag aagttgcact tcttaacaaa caacgtgcgc gtcttgaaaa
    40561 attcttgggc ggtatcgaag atatgcctcg tatcccagat gtgatgtacg tagttgaccc
    40621 acataaagag caaatcgctg ttaaagaagc taaaaaattg ggaatcccag ttgtagcgat
    40681 ggttgacacc aatactgatc cagatgatat cgatgtaatc atcccagcta acgatgacgc
    40741 tatccgtgct gttaaattga tcacagctaa attggctgac gctattatcg aaggacgtca
    40801 aggtgaggat gcagtagcag ttgaagcaga atttgcagct tcagaaactc aagcagattc
    40861 aattgaagaa atcgttgaag ttgtagaagg tgacaacgct taatttatac aaatagtaat
    40921 tacctaggag ggcggggctt agcccggctc tcctattttc aaaaaatata ggagaattaa
    40981 aatggcagaa attacagcta aacttgtaaa agagttgcgt gaaaaatctg gtgccggtgt
    41041 tatggacgct aaaaaagcgc ttgtagaaac agacggtgac atcgaaaaag cgattgaatt
    41101 gcttcgtgaa aaaggtatgg ctaaggcagc taagaaagct gaccgtgttg ctgcagaagg
    41161 tttgactggt gtttatgtta acggtaatgt tgcagcagtt attgaagtaa acgctgaaac
    41221 tgacttcgtt gcaaaaaacg ctcaattcgt tgaattggta aatactacag ctaaagtcat
    41281 tgctgaagga aaacctgcta ataatgaaga agctcttgct ttgataatgc cttcaggtga
    41341 aactcttgaa gctgcatacg tatctgcaac agcaactatc ggagagaaaa tctcattccg
    41401 tcgctttgca ttgattgaaa aaacagacgc acaacacttt ggagcatacc aacataacgg
    41461 tggacgtatc ggtgttattt cagttgttga aggtggagac gaagcacttg ctaaacaatt
    41521 gtcaatgcac atcgcagcga tgaaaccaac agttctttct tacaaagaat tggatgagca
    41581 attcgttaaa gatgagttgg cacaattgaa tcacgttatc gaccaagaca acgaaagccg
    41641 tgcaatggtt aataaaccag ctcttccaca cttgaagtat ggatcaaaag ctcaattaac
    41701 tgatgatgtt attgctcaag ctgaagctga catcaaagct gaattggctg cagaaggcaa
    41761 accagaaaaa atctgggaca aaattatccc aggtaaaatg gatcgcttca tgcttgataa
    41821 tactaaagtt gaccaagctt acacacttct tgcacaagtt tacatcatgg atgacagcaa
    41881 gacagttgaa gcataccttg aatcagttaa cgcttcggta gttgagtttg ctcgctttga
    41941 agttggtgaa ggtatcgaga aagctgcaaa cgacttcgaa gctgaagttg cagctacaat
    42001 ggcagcagcc ttgaataact aataatagaa aggaagcaat ttgcttcctt ttttgtatag
    42061 ggcttatatt tttcctgaaa ttgtttttcc cccagtctca ctcgattaga ataattaaaa
    42121 caaaaagccc tataataagg gctaagtgca tttaggagac tatacttcaa attcatagta
    42181 agcgcgtcat aacaaggtat ctatcctaat tccccaactt ccagtatact agataaaaaa
    42241 taattatcgg aggaaaggta ttgtactatg atgattcaac taagtgattt aggtcaagtt
    42301 cacattgttt gtggcaagac agatatgagg cagggaatag actcattagc ctatgtagtt
    42361 aaaacccact ttgaattgga ttctttctcc ggtcaagtct ttctcttttg tggtggacgt
    42421 aaagaccgct ttaaagtcct ttactgggat ggtcaaggat tttggctact atataaacgc
    42481 tttgagaacg gaaaactgac ttggctaagt acagaaaagg atgtcaaagc tctcgcacct
    42541 gaacaagtag actagcttat gaagggcttt tctatcactc caaaaatata gtagattgaa
    42601 actagaatag tacacctctg cttctaaaac attgttagaa atcgatttga ctgtcctgat
    42661 cgatttgtcc tgttcttatt tcattttact ataaatccat cagaaagtcg tgatttctat
    42721 tgaaatgagg actttctttt tacactcatc tgctttcaaa aagcattcta gtccgtctcc
    42781 gattaacgat ggactttatc acctccttct ctagtccttg tatgacatct tccaaagttc
    42841 gaaaggcttt attcttaaat ccacgtttac gaatct
//
LOCUS       DAVBDG010000011        42038 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBDG010000011 DAVBDG010000000
VERSION     DAVBDG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.011"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..300
                     /locus_tag="VQ526_000533"
     CDS             <1..300
                     /locus_tag="VQ526_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2554294.1"
                     /translation="ILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSQDNGMMESFF
                     GILKSEMFYGYEKSFQSLKQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(345..986)
                     /gene="plsY"
                     /locus_tag="VQ526_000534"
     CDS             complement(345..986)
                     /gene="plsY"
                     /locus_tag="VQ526_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2554295.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            1122..3065
                     /gene="parE"
                     /locus_tag="VQ526_000535"
     CDS             1122..3065
                     /gene="parE"
                     /locus_tag="VQ526_000535"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2554296.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            3485..5956
                     /gene="parC"
                     /locus_tag="VQ526_000536"
     CDS             3485..5956
                     /gene="parC"
                     /locus_tag="VQ526_000536"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2554297.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            6088..7110
                     /locus_tag="VQ526_000537"
     CDS             6088..7110
                     /locus_tag="VQ526_000537"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2554298.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            7166..7870
                     /locus_tag="VQ526_000538"
     CDS             7166..7870
                     /locus_tag="VQ526_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2554299.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNGTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            7935..8624
                     /locus_tag="VQ526_000539"
     CDS             7935..8624
                     /locus_tag="VQ526_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2554300.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            8621..9544
                     /locus_tag="VQ526_000540"
     CDS             8621..9544
                     /locus_tag="VQ526_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2554301.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9559..10203
                     /gene="pcp"
                     /locus_tag="VQ526_000541"
     CDS             9559..10203
                     /gene="pcp"
                     /locus_tag="VQ526_000541"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2554302.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10371..10601
                     /locus_tag="VQ526_000542"
     CDS             10371..10601
                     /locus_tag="VQ526_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2554303.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            10651..10731
                     /locus_tag="VQ526_000543"
     tRNA            10651..10731
                     /locus_tag="VQ526_000543"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10685..10687,aa:Tyr,seq:gta)
     gene            10741..10812
                     /locus_tag="VQ526_000544"
     tRNA            10741..10812
                     /locus_tag="VQ526_000544"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10773..10775,aa:Gln,seq:ttg)
     gene            10974..12176
                     /gene="rpsA"
                     /locus_tag="VQ526_000545"
     CDS             10974..12176
                     /gene="rpsA"
                     /locus_tag="VQ526_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2554304.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            12262..12759
                     /locus_tag="VQ526_000546"
     CDS             12262..12759
                     /locus_tag="VQ526_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2554305.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            12759..14414
                     /gene="dnaX"
                     /locus_tag="VQ526_000547"
     CDS             12759..14414
                     /gene="dnaX"
                     /locus_tag="VQ526_000547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2554306.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            14442..14636
                     /locus_tag="VQ526_000548"
     CDS             14442..14636
                     /locus_tag="VQ526_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2554307.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            14786..15556
                     /gene="sufC"
                     /locus_tag="VQ526_000549"
     CDS             14786..15556
                     /gene="sufC"
                     /locus_tag="VQ526_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2554308.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            15584..16846
                     /gene="sufD"
                     /locus_tag="VQ526_000550"
     CDS             15584..16846
                     /gene="sufD"
                     /locus_tag="VQ526_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2554309.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            16857..18083
                     /locus_tag="VQ526_000551"
     CDS             16857..18083
                     /locus_tag="VQ526_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2554310.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSAHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            18070..18510
                     /locus_tag="VQ526_000552"
     CDS             18070..18510
                     /locus_tag="VQ526_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2554311.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            18564..19976
                     /gene="sufB"
                     /locus_tag="VQ526_000553"
     CDS             18564..19976
                     /gene="sufB"
                     /locus_tag="VQ526_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2554312.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(20175..21416)
                     /locus_tag="VQ526_000554"
     CDS             complement(20175..21416)
                     /locus_tag="VQ526_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2554313.1"
                     /translation="MKKIFLTLLTVSLLGGVSTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            21519..22628
                     /locus_tag="VQ526_000555"
     CDS             21519..22628
                     /locus_tag="VQ526_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2554314.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            23274..23621
                     /gene="ssrA"
                     /locus_tag="VQ526_000556"
     tmRNA           23274..23621
                     /gene="ssrA"
                     /locus_tag="VQ526_000556"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(23804..23891)
                     /locus_tag="VQ526_000557"
     tRNA            complement(23804..23891)
                     /locus_tag="VQ526_000557"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23855..23857),aa:Ser,seq:gga)
     gene            24053..24793
                     /locus_tag="VQ526_000558"
     CDS             24053..24793
                     /locus_tag="VQ526_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2554315.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            24790..25701
                     /gene="pfkB"
                     /locus_tag="VQ526_000559"
     CDS             24790..25701
                     /gene="pfkB"
                     /locus_tag="VQ526_000559"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2554316.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            25698..27650
                     /locus_tag="VQ526_000560"
     CDS             25698..27650
                     /locus_tag="VQ526_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2554317.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            27814..30039
                     /locus_tag="VQ526_000561"
     CDS             27814..30039
                     /locus_tag="VQ526_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2554318.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(30084..30512)
                     /locus_tag="VQ526_000562"
     CDS             complement(30084..30512)
                     /locus_tag="VQ526_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554319.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            30650..31792
                     /locus_tag="VQ526_000563"
     CDS             30650..31792
                     /locus_tag="VQ526_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2554320.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            31801..33015
                     /gene="thiI"
                     /locus_tag="VQ526_000564"
     CDS             31801..33015
                     /gene="thiI"
                     /locus_tag="VQ526_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2554321.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            33150..33974
                     /locus_tag="VQ526_000565"
     CDS             33150..33974
                     /locus_tag="VQ526_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2554322.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            33996..34735
                     /locus_tag="VQ526_000566"
                     /pseudo
     CDS             33996..34735
                     /locus_tag="VQ526_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            34748..35914
                     /locus_tag="VQ526_000567"
     CDS             34748..35914
                     /locus_tag="VQ526_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2554323.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            36422..37915
                     /locus_tag="VQ526_000568"
     CDS             36422..37915
                     /locus_tag="VQ526_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2554324.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVDNHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            37928..38317
                     /locus_tag="VQ526_000569"
     CDS             37928..38317
                     /locus_tag="VQ526_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2554325.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSEFACEIAIKVWRNSLKFSII"
     gene            38347..41697
                     /locus_tag="VQ526_000570"
     CDS             38347..41697
                     /locus_tag="VQ526_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET2554326.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSDHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSKEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT        12745 a         7556 c         9306 g        12431 t
ORIGIN      
        1 attctccata gtgaccaagg ctggcaatat caacacgatt cttatcatcg gttcttagag
       61 agtaagggaa ttcaagcatc catgtcacgc aagggaaaca gccaagacaa tggtatgatg
      121 gagtccttct ttgggattct gaaatcggaa atgttttacg gttatgagaa gtcgtttcag
      181 tcgcttaagc aattggaaca agccattata gactatattg attactacaa caacaagaga
      241 attaaggtaa aactaaaagg acttagccct gtgcaataca gaactaaatc cttcggataa
      301 attaattgtc taacttttgg ggggcagttc acagaactgg cattttattt tttaggatct
      361 tgatgggtta ggttcaatcc ccaagggacc aaattttcag ttttattttt gatacgagct
      421 atattgtcct tatgacgaat gataatcaaa ctagcaagtg ctaagataat agcgatgaag
      481 agagagtcat agttactcag gataaaacca aaaagtggaa agagcagaac cccgataaca
      541 gccgcgatcg atgctgtgac actagacagt gaaatcatac tgccaagata gagagctcca
      601 aagaagataa tcgcaaggta gagacagaag ataggcgcaa atccgaaaat tactccagca
      661 ctggttgcga cagccttacc acctttaaat cctgcaaaga tagggaaggt atggccgata
      721 acagccaaaa gtccaaagat gagaggagaa acgccttgta gatgaaaaat aatcggaagc
      781 agcgttgcta gggttccttt gaaaaagtca atcacaaagg ttgccatacc agctttctta
      841 cctaaaatgc ggaaggtatt ggtcgttcca gtgttaccag aaccatgctc gcgtagattg
      901 atttgaaaga atacttgtcc aatccagaga ccagatggaa tcgaacccag cagataggct
      961 aggattaata aaactattgt aatcatactc ctattatatc atgaattggg aaagaaaagc
     1021 agagaatctc tgctaaaatt gtcacatcgg agaaagaaaa attttgcaaa atccttggaa
     1081 aacctgtaga atagtaaaga tgaacgaata ggaggttcct tgtgtcaaaa aaggaaatca
     1141 atattaacaa ttataatgat gatgctattc aggtgctaga agggttggat gcggtccgaa
     1201 aacgtccagg gatgtatatt ggatcgaccg atggcgctgg tcttcatcac ctagtttggg
     1261 aaatcgttga taatgcagtc gatgaagcct tgtctgggtt tggtgatcgt atcgatgtaa
     1321 ccatcaataa agacggtagt ctaacggttc aagaccatgg acgtgggatg ccgacaggta
     1381 tgcacgctat gggaattcca actgttgagg ttatctttac cattcttcat gccggaggga
     1441 aattcggtca aggtggctat aagacatcag gtggacttca cggagtgggt tcttccgttg
     1501 ttaacgccct ttctagctgg ttagaagttg aaattacccg tgatggcgca gtttacaagc
     1561 aacgtttcga aaatggtgga aaacctgtca cgactttgaa gaaaatcggt acagcaccca
     1621 agtctaaaac aggcaccaaa gttactttta tgcctgacgc gactatattt tctacgacag
     1681 atttcaagta caataccatt tcagagcgcc ttaatgaatc agcctttctc ttgaaaaatg
     1741 tgaccttgtc tttaacggac aagcgaacag atgaagcgat tgagttccac tatgagaatg
     1801 gagtacaaga ttttgtttct tatctcaacg aagataagga aatcttgacg ccagttcttt
     1861 actttgaagg ggaagacaat ggttttcaag tggaagtagc cctccagtac aatgacggat
     1921 tctcagataa cattctatcc tttgtcaata acgttcgcac caaggacggt ggaacgcacg
     1981 agacaggact caagtctgcc attaccaagg tcatgaatga ctatgcacgt aaaacaggtc
     2041 ttctcaagga aaaagataaa aaccttgaag gttcagacta tcgtgaggga ctagcggccg
     2101 ttctttctat cttagttcct gaagaacact tgcagtttga aggacagacc aaggataaac
     2161 taggaagccc cctagctcgc ccagttgtgg atggaatagt ggctgataag ttgacctttt
     2221 tccttatgga aaatggggaa ttagcttcta acctcatccg caaggctatc aaggcccgtg
     2281 atgctcgtga agcagcacgt aaggcgcgtg atgagagccg aaatgggaag aaaaacaaga
     2341 aagataaggg cttgttgtct gggaaattga ccccagccca atctaagaat cctgctaaga
     2401 atgaactcta tctagttgag ggggactctg ccggtggttc tgccaaacaa ggtcgtgacc
     2461 gcaagttcca ggctattcta cctcttcgtg gtaaggttat caatacagcc aaggccaaga
     2521 tggcggatat cctcaaaaat gaagagatca ataccatgat ttataccatt ggtgcgggtg
     2581 ttggagcaga cttctctatt gaagatgcca actatgataa gatcattatc atgaccgatg
     2641 cggataccga cggtgcccat atccagacct tgctcttgac atttttctac cgttacatgc
     2701 gtccgctagt cgaggcaggt catgtctata ttgccctccc acctctttac aagatgtcca
     2761 aaggtaaagg aaagaaagaa gaagtggcct acgcttggac ggacggagaa ctagaagaac
     2821 tccgtaaaca gttcggtaaa ggcgctaccc tccaacgata caaaggactt ggtgagatga
     2881 atgcggacca gctctgggaa acaaccatga acccagaaac acgtaccctc atccgtgtca
     2941 caattgaaga tttagcgcgc gccgaacgcc gcgtcaatgt tctcatggga gataaggtag
     3001 aaccacgccg taaatggatt gaagataatg tcaagtttac gctagaagaa gcgacagtgt
     3061 tttaatgaaa gaaaataata tgttagtaaa ctggaaatta gagtcggatg tgaatgacta
     3121 tgtgaaaaag cagtttgaaa atcttggttt gaaaaaattg caggattatt aatgaagaat
     3181 ctgctatgag tgcttatctc aaagaggcgc ttaagggagc ggctaagaca caaactaaga
     3241 ctaattttgg aaaaccagat tttcatattg aaaaatacaa acaggatagt ttggttactt
     3301 atcgtaactg aacacgggag gaattcccgt aaaatatctt ttactgcata aaaacctact
     3361 ctacattctt tgaaaggagt tgaacacgcc ctagatactg tgtgaaaaag ataaattctc
     3421 ttgtgagttt gcttacttca agaattttct attttcactt ggtattttat gggctttgta
     3481 tcttatgtct aacattcaaa acatgtccct ggaggacatc atgggagagc gctttggtcg
     3541 ctactccaag tacattattc aagaccgggc tttgccagat attcgtgatg ggttgaagcc
     3601 ggttcagcgc cgtattcttt attctatgaa taaggatagc aatacttttg acaagagcta
     3661 ccgtaagtcg gccaagtcag tcgggaacat catggggaat ttccacccac acggggattc
     3721 ttctatctat gatgccatgg ttcgtatgtc acagaactgg aaaaatcgtg agattctagt
     3781 tgaaatgcac ggtaataacg gttctatgga cggagatcct cctgcggcta tgcgttatac
     3841 tgaggcacgt ttgtctgaaa ttgcaggtta ccttcttcag gatatcgaga aaaatacagt
     3901 tccttttgca tggaactttg acgatacgga gaaagaacca acggtcttgc cagcagcctt
     3961 tccaaacctc ttggtcaatg gttcgactgg gatttcggct ggttatgcca cagacattcc
     4021 tccccataat ttagctgagg tcatagatgc tgcagtttac atgattgacc acccaactgc
     4081 aaagattgat aaactcatgg aattcttgcc tggaccagac ttccctacag gggctattat
     4141 tcagggtcgt gatgaaatca agaaagctta tgagactggg aaagggcgcg tggttgttcg
     4201 ttccaagact gaaattgaaa agctaaaagg tggtaaggaa caaatcgtta ttactgagat
     4261 tccttatgaa atcaataagg ccaatctagt caagaaaatc gatgatgttc gtgttaataa
     4321 caaggtagct gggattgctg aggttcgtga tgagtctgac cgtgatggtc ttcgtatcgc
     4381 tatcgaactt aagaaagacg ctaatactga gcttgttctc aactacttat ttaagtacac
     4441 cgacctacaa atcaactaca actttaatat ggtggcgatt gacaatttca cacctcgtca
     4501 ggttgggatt gttccaatcc tgtctagcta catcgctcac cgtcgagaag tgattttggc
     4561 gcgttcacgc tttgacaaag aaaaggctga gaaacgtctc catatcgtcg aaggtttgat
     4621 tcgtgtgatt tcgattttgg atgaagtcat tgctcttatc cgtgcttctg agaataaggc
     4681 ggacgccaag gaaaacctca aagttagcta tgattttacg gaagaacagg ctgaggctat
     4741 cgtaactttg caactgtacc gtttgaccaa taccgatgtg gttgtcttgc aggaagaaga
     4801 agcagagctt cgtgagaaga ttgctatgct ggcggctatt atcggtgatg aaaggactat
     4861 gtacaatctc atgaagaaag aacttcgtga ggtcaagaag aaatttgcaa ctcctcgttt
     4921 gagttcttta gaagacactg cgaaagcaat tgagattgat acagctagtc ttatcgctga
     4981 ggaagatacc tacgtcagcg tgaccaaggc aggttacatc aagcgtacca gtccacgttc
     5041 ctttgcggct tccaccttgg aagaaattgg caagcgtgat gatgaccgtt tgatttttgt
     5101 tcaatctgcc aagacaaccc agcacctctt gatgttcaca agtcttggaa atgtcatcta
     5161 cagaccaatc catgagttgg cagatattcg ttggaaggac atcggagagc atctgagcca
     5221 aaccatcaca aactttgaaa cgaatgaaga aatcctttat gtggaagtac tggatcagtt
     5281 tgacgatgcg acaacctact ttgcagtgac tcgccttggt caaatcaaac gggtagagcg
     5341 aaaagaattc actccatggc ggacctatag atctaagtct gtcaagtatg ctaagctcaa
     5401 agacgataca gatcagattg tagcagtggc tccgattaaa ctagatgatg ttgtcttggt
     5461 tagtcaaaat ggttatgccc tgcgtttcaa tatcgaagag gttccggttg tcggtgctaa
     5521 ggcagcaggt gtcaaggcta tgaatttgaa agaagatgat gtcctccaat ctggctttat
     5581 ctgtaatact tcgtccttct acctcttgac ccagcgtgga agcttgaaac gtgtttctat
     5641 tgaggaaatt ctagcaacca gccgtgccaa acgaggatta caagtcttgc gtgagttgaa
     5701 aaacaaaccg catcgtgtct tcttggcagg agcagttgca gagcaaggat ttgttggcga
     5761 tttcttcagt acggaagtgg atgtgaacga ccaaactctg cttgtccaat ccaataaagg
     5821 aacaatctat gaaagccgat tgcaagactt gaacttgtca gaacgcacta gcaatggaag
     5881 cttcatttct gacacgattt cagatgaaga agtttttgac gcttatcttc aggaagtagt
     5941 tactgaagat aaataagtga aatgaagaat cagttctaag agctggtttt tattggagaa
     6001 attttctgaa aattacaaaa tatacttgct attttagaaa aatagtgtag aatataagaa
     6061 ataggttttt agaataagga gtggaatatg acagtaacga ttgattggga aaacctcggt
     6121 ttttcctata tgaaattacc ttatcgctat cttgctcatt tcaaaaatgg acaatgggat
     6181 caaggagagc ttacagagga tgcaactttg catatttcag agtcttctcc aagtcttcac
     6241 tatggacaac aagcatttga aggtttgaaa gcttatcgta ctaaggatgg cagtgttcaa
     6301 ctgttccgtc ctgatgaaaa tgctaaacgt ctgcaacgta catgtgaccg tctcttgatg
     6361 ccacaagttc cgacagacat gtttgtagaa gcttgtaaag cagttgtccg tgcgaatgaa
     6421 gaatacgtac caccatacgg aacaggtgga accttatatc ttcgccctct tttgattggt
     6481 gtcggagata ttatcggggt aaaaccggca gaagagtaca ttttcaccat ctttgctatg
     6541 ccagttggaa attactttaa aggtggtttg gtcccaacca acttcttgat tcaggatgag
     6601 tacgaccgtg cagcaccaaa tggtacaggt gcggctaagg ttggtggaaa ctatgctgca
     6661 agtctcttac caggaaaaat ggccaagtca cgtcatttct cagatgttat ctatctggac
     6721 ccatcaactc atacaaagat tgaagaagtc ggatcagcta acttctttgg aattacagct
     6781 gataatgaat ttgtaacacc attgagtcca tctatcttgc catctattac caagtattcc
     6841 ttgctttatt tggcagaaca tcgcttggga ttaactccta ttgagggtga tgttccaatt
     6901 gataatcttg accgttttgt agaggcaggt gcctgtggta cagcagcggt tatttctcca
     6961 attggaggta ttcaacatgg tgatgatttc catgtattct atagtgaaac agaagtaggt
     7021 cctgtgacgc gtaaattata taatgaattg acgggtattc agtttggcga tattgaagcg
     7081 ccagaaggtt ggattgtaaa agtagattaa aataaaccaa aggagatttt ttatgaaata
     7141 gaaaaagtgg ctcttaacag caggagtggt cctgagcacg tcagctattt tagtggcttg
     7201 tggaaaaact gataaagaag cagatgcacc gacaacattt tcttatgtct atgcagtaga
     7261 tccagcatca ttgggctaca gtatagcgac tcgaacatcg aggacagacg ttattggaaa
     7321 tgttattgat ggtttgatgg aaaatgataa atacggcaat gttgctcctt ctcaaaaaga
     7381 ctatgatttg aacggtacag gatgggctcc aagctatcaa gatccagcgt cttacttgaa
     7441 tattatggat ccaaaatctg gttctgccat gaaacacctt ggcattacga aaggaaaaga
     7501 taaggatgtt gtagctaaac ctggtttgga taaatataag aaattgttag aagatgctgt
     7561 ttctgagacc actgacctag agaagagata tgaaaaatat gccaaagctc aagcttggtc
     7621 gacagatagt tcattattga tgccaacagc ttcatctggt ggttttccag ttgtaagtaa
     7681 cgtagtacca ttctcaaaac catactcaca agttggtatt aagggggaac catatatctt
     7741 taaaggaatg aaattgcaaa aagatattgt tacaacaaaa gaatataacg aggtttttaa
     7801 aaaatggcaa aaagaaaaat tggaatccaa tagcaaatac caaaaagaac tagaaaaata
     7861 cattaaataa ggaatggtat tgatcttgat aaaattttta aaatactgtc attttgaata
     7921 taaaggagtt tgatatggag tggattagat taataggaat agcaatcatt gttgtgggtt
     7981 ttattttaaa atttgataca attgcaacag tagtcttagc tggtttggtt acagctttag
     8041 tttcaggtgt ttctctcgtt gaatttttgg agattttggg aaaagaattt agcaatcagc
     8101 gagtgctcac gatttttatg gttaccttgc ctcttgtggg gctgtcagaa acctttggac
     8161 tcaagcaacg atcaatcgat ttgattcgaa agattaaagg tctgacagtt ggaaacttct
     8221 atacagttta tttctttatt cgagagttag ctggtttctt ttcaattcgt ctaggaggac
     8281 accctcagtt tgtcagacct ttggttcaac ctatgggaga agcagctgca gagtctcaat
     8341 taggtagaaa gttaacagag gttgaagatg agacaataaa agcgcgtgcg gctgcgaatg
     8401 aaaattttgg aaatttcttt gctcaaaata cgtttgtagg tgctggggga gtcctcttga
     8461 tagggggaac attagatcag ttaggctatg aaagcaatta tgcagggatt gcttctacat
     8521 ctattattgt tgctgttata acacttattg tagtggggat ttacaattat ttatttgata
     8581 aaaaattgat atcaaaaaag actaggggag gagaacaaaa atgacagagt tggcaaagca
     8641 actattagag ttgacctata ttgtgattgg ttgtcaattt ctccatacag cctattgtag
     8701 ttataaagat aaaacaaacc cagttcgact tgggacatct gcattttgga ctctattgtc
     8761 tattacgttt ataggtggtt cctatatgcc aaatatgagt attggtatta ttgtaatcct
     8821 attatcgctg ttaacattgt ttaagcaagt ccgtatcgga accttgccat ccttagatga
     8881 aatgaaagcc aatattgaat ctaacaggtt gaaaaataaa atttttattc cagttatgct
     8941 gatggcaata cttgcgttgg tcttagcgca aatgattcca gaatttagca agatttcgat
     9001 tagccttgcc gccttgtttg ctacaatttc tgttcttgtg attaccaata gtcaccctaa
     9061 gagtctgtta tcagaaaata atcgaatgac tcagcaagtt tcaacaagtg gtattgttcc
     9121 tcaattatta ggggctttgg gggctatttt tactgtagca ggtgttggtg atgttatctc
     9181 tcatctgatt agcggtattg ttccttcaga tagtcgcttt ataggagttt tggcttatgt
     9241 tcttggaatg gttctattca caatgattat gggaaatgct tttgcagcat tcaccgttat
     9301 tacagcaggt gttggagttc cctttgtatt tgctctggga gctaatccaa ttgtggctgg
     9361 tgctcttgcc atgacagcag gttattgtgg gaccttattg accccaatgg ctgctaattt
     9421 taacgctcta ccagcagcat tgatggatat gaaagatcag aatggcgtta taaaggctca
     9481 agcaggtgtt gctctagtaa tgattgttat tcacatattc ttaatgtact ttctcgcatt
     9541 ttagtaaagg aaaataaaat gaaaatattg gttacaggtt ttaatccttt tggaggtgaa
     9601 aagattaatc cagctttgga ggctgtaaaa ttattaccat ctgagattaa tggggctgaa
     9661 gttcgctggg tagaaattcc aacggttttt tataagtcgt cagaagtttt agaggcagaa
     9721 atattacgat atcaaccaga tgctgtactt tgtattggac aagcaggcgg caggaccggc
     9781 ttaacacctg aacgagtggc tattaatcaa gatgatgctc gcatacctga taacgaaggc
     9841 aatcaaccaa ttgatacacc gattcgtatt gatggagcat cggcctattt tagtagttta
     9901 cctatcaaag cgatggtaca agctatcaaa aaacagggac ttccggcagt tgtatccaat
     9961 agtgcaggaa cctttgtttg caatcatttg atgtaccaag ctctttattt agtggataaa
    10021 aaattcccta atatgagagc aggtttcatg catattccat atatgatgga acaagtagta
    10081 aataagccga atactgcagg aatgagtcta tgtgatattg taagaggcat agaggtagct
    10141 attgaagcta ttgtagacta caaagataag gatttgcagt tagtaggcgg tgagactcac
    10201 tgataaccta aaaaggatag tcaattatgc ttgtttacta actattaact atgctaaatc
    10261 aattgaggtt gtttacataa aactctatat cagagaagcc tgatatagag ttttttcttg
    10321 ctagttttag gattttcttg taaaatagaa aaagtgaaga gaggtatgaa atgagcaaga
    10381 aagataaaaa aatcgaaatt caagtagcgg atgccaaagt taatgttggt aaagacagtt
    10441 ttgaaggtta tacattgact atcggtaaaa aagttatcgg agaaattgcc gaattagacg
    10501 gacaatttgc cattataaag aatgggaatg tcgatagttt ttataaaaaa ttggaaaaag
    10561 ctgtggaaat tttgattgaa aattataatt tagcaaaata agtcttgttt ttttgaaatt
    10621 ttcatgatat aatagtccat gttgattgta ggagagatag cgaagaggct aaacgcggcg
    10681 gactgtaaat ccgctccttc gggttcgggg gttcgaatcc ctctctctcc atttcattaa
    10741 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
    10801 cagctacccc agttcttagg taataatcaa gataaaaagc aaaatatctt agggtatttt
    10861 atttttataa ttgaaagacg tgaatgatat gaacatgtcc ttgcgggtgc ttaggaaaaa
    10921 aattataagt atgtcaagtt taagaaaaac ttgattgttg gaggattttt tagatgaacg
    10981 aatttgaaga tttgctaaat agcgttagtc aagttgagac tggtgatgtt gttagtgctg
    11041 aagtattgac agttgatgcg actcaagcta acgttgcaat ctctggaact ggtgttgaag
    11101 gtgtcttgac tcttcgcgaa ttgacaaacg atcgtgatgc agatatcaat gactttgtta
    11161 aagtaggaga agtattggat gttcttgtac ttcgtcaagt agttggtaaa gatactgata
    11221 cagttacata ccttgtatct aaaaaacgcc ttgaagctcg caaagcatgg gacaaacttg
    11281 ttggtcgcga agaagaagtt gttactgtta aaggaacgcg tgccgttaaa ggtggacttt
    11341 cagtagaatt tgaaggtgtt cgtggattta tcccagcttc aatgttggat actcgtttcg
    11401 tacgtaacgc tgagcgtttt gtaggtcaag aatttgatac taaaatcaaa gaagttaacg
    11461 ctaaagaaaa ccgcttcatc ctttcacgtc gtgaagttgt tgaagcagct actgcagcag
    11521 ctcgcgctga agtattcggt aaattggctg ttggtgatgt tgtaactggt aaagttgctc
    11581 gtatcacaag cttcggcgct ttcatcgacc ttggtggtgt tgacggattg gttcacttga
    11641 ctgaattgtc acatgaacgt aacgtatcac caaaatcagt tgtaactgtt ggtgaagaaa
    11701 ttgaagtgaa aatccttgat cttaacgaag aagaaggacg tgtatcactt tcacttaaag
    11761 caacagtacc aggaccatgg gatggcgttg agcaaaaatt ggctaaaggt gatgtagtag
    11821 aaggaacagt taaacgtttg actgacttcg gtgcatttgt tgaagtattg ccaggtatcg
    11881 atggacttgt tcacgtatca caaatttcac acaagcggat tgaaaatcca aaagaagctc
    11941 ttaaagttgg tcaagaagtt caagttaaag ttcttgaagt taacgcagat gcagaacgcg
    12001 tgtcactttc tattaaagct cttgaagaac gtccagccca agaagaagga caaaaagaag
    12061 aaaaacgtgc tgctcgtcca cgtcgtccaa gacgtcaaga aaagcgtgat ttcgaacttc
    12121 cagaaacaca aacaggattt tcaatggctg atttgtttgg tgatatcgaa ctttaatcaa
    12181 attgaaaatt cacaaaatcc tttgtttact aaacaaggga tttttctttt gtctctgctc
    12241 cttttttgat ataatagttc tatgttaaaa tcagaaaaac aatcacgtta tcaaatgtta
    12301 aatgaagaat tgtccttcct attggaaggc gaaaccaatg ttttggctaa tctttccaac
    12361 gccagtgctc ttataaaatc acgttttcct aataccgtat ttgcaggctt ttatttgttc
    12421 gatggaaagg aattggtttt aggtcccttc caaggaggtg tttcctgcat ccgtattgca
    12481 ctaggcaagg gtgtttgtgg tgaggcagct cactttcagg aaactgttat tgttggagat
    12541 gtgacgacct atctcaacta tatttcttgt gatagtctag ctaaaagtga aattgtggtg
    12601 ccgatgatga agaatggtca gttacttgga gttctggatc tggattcttc agagattgag
    12661 gattacgatg ctatggatcg agattatttg gaacaatttg tcgctatttt gcttgaaaag
    12721 acaacatggg actttacgat gtttgaggaa aaatcttaat gtatcaagca ctttatcgaa
    12781 aatatagaag tcaaaacttc tcccagttag ttggtcaaga agttgtggct aagactctta
    12841 aacaagcggt ggagcaagag aaaataagtc acgcttatct tttttctggt cctcgtggaa
    12901 cgggaaaaac cagtgttgct aaaatctttg ccaaggctat gaactgtccc aatcaagtgg
    12961 gtggcgaacc ttgcaataac tgctatattt gtcaagcagt gacggacggt agtttagaag
    13021 atgtcattga aatggatgca gcttctaata atggggtaga tgaaattcgc gaaattcgtg
    13081 ataaatctac ctatgcgcct agccttgctc gttataaggt ttatatcata gatgaggttc
    13141 acatgctgtc tacaggggct tttaatgccc tcctaaagac gctggaagaa ccaacacaga
    13201 atgtagtctt tattttggcc actactgaat tgcacaagat tcctgctact attctatccc
    13261 gtgtgcaacg ttttgagttt aaatcaatta agacacagga tattaaggaa catattcact
    13321 atatcttaga aaaagaaaat atcagttctg aaccagaggc tgtggaaatc attgccagac
    13381 gggcggaagg tggaatgcgg gacgccttgt ctattttgga tcaagccctg agtttgacac
    13441 agggaaatga gctgacgact gctatctctg aagaaattac tggcaccatt agcctatcag
    13501 ccttggatga ttatgtggcg gccttgtctc aacaggatgt tcccaaagct ttgtcttgct
    13561 tgaatcttct ttttgacaat ggtaagagca tgactcgttt tgtgaccgat cttttgcact
    13621 atttaagaga cttgttaatt gttcaaacag ggggagaaaa tactcatcat agttcagtct
    13681 ttgtagaaaa tttggcactt cctcaaaaaa atctgtttga aatgattcgc ttagcaacag
    13741 tgaatttagc agatattaag tctagtttgc agcccaagat ttatgctgaa atgatgaccg
    13801 tccgtttggc ggaaatcaag cccgaaccag ctctatcagg agcggttgaa aatgaaattg
    13861 ctacgctgag acaggaagtt gcccgtctca aacaagagct ttctaatgca ggtgcggttc
    13921 ctaaacaagt tgcaccagct cctagtcgac cagctacggg caaaacagtc tatcgtgtcg
    13981 atcgcaataa agtgcaatct atcttacaag aggccgtcga aaatcctgat ttagcacgtc
    14041 aaaatctaat tcgtttgcag aatgcctggg gagaggtaat tgaaagtcta ggtgggccgg
    14101 acaaggctct gctagttggt tctcaaccgg ttgctgccaa tgaacaccat gctattcttg
    14161 cttttgagtc taacttcaat gctggtcaaa ctatgaaacg agacaatctc aataccatgt
    14221 ttggtaatat cctcagtcag gcggcaggtt tttcacctga gattttagct atttccatgg
    14281 aggaatggaa agaagttcgc gcagcctttt cagccaaagc caaatcttct caaactgaaa
    14341 aagaagtaga agaaagcctg attccagaag gatttgaatt tttggctgat aaagtgaagg
    14401 tagaggaaga ctaaagaaag atttcatgat acaataagtt tatgaataaa caacaattta
    14461 ttattatggc gctatttaca gctgctgaga cctatttttt caatgaagcc tggatgactg
    14521 gccgctatat tatggcagcc ttttgggcaa ttttactctt tagaaatttc cgagtcagtt
    14581 atgtgatggg taaaattgtt gatgtcatcg atcagcattt taataggaaa gactagccct
    14641 cagcttccag acaaaatcaa agccttttag gctttttttt gttatactag aaaagtatat
    14701 ttatagaatt tttgctctat ttctggggaa atcagacgtt tttctagtaa gtactgtaaa
    14761 agttttgaaa aagaaaggaa ctatcatgtc agtaatagag atcaaagatc ttcacgttga
    14821 gattgaagga aaagaaattt taaaaggggt taacctgacc ctgaaaacag gagaaattgc
    14881 cgctatcatg ggaccaaatg gtacaggtaa atcgactctt tctgccgcta tcatgggaaa
    14941 tccaaactat gaagtaacta aaggtgaagt tttgtttgat ggcgtaaaca tccttgagtt
    15001 ggaagtggat gagcgtgcgc gtatgggact tttccttgct atgcaatacc catcagaaat
    15061 ccctggaatt accaatgctg agtttcttcg tgccgctatg aatgcgggta aagaagatga
    15121 tgagaagatt tcagttcgtg agtttattac taagctagat gaaaaaatgg aattgctcaa
    15181 catgaaagaa gaaatggcag agcgttacct caacgaaggc ttctctggtg gtgagaaaaa
    15241 acgcaatgaa attcttcaac ttttgatgtt ggagccaaca tttgctcttt tggacgagat
    15301 tgactcaggt cttgatattg acgctcttaa agttgtgtct aaaggtgtca atgccatgcg
    15361 tggtgaaggt tttggtgcta tgatcatcac tcactaccaa cgtcttttga actatatcac
    15421 acctgatgtg gtacacgtga tgatggaagg tcgtgttgtc ctttctggtg gtccagaatt
    15481 ggctgcgcgt ttggaacgtg aaggatacgc aaaattagct gaagaacttg gctacgacta
    15541 caaggaagaa ttgtaatttc ctcgtatctt ttaggagaag taaatgacta gagaaaatat
    15601 taaacttttt tcagaaatgc acgctgaacc aagctggttg gctgatctcc gtcaaaaagc
    15661 ttttgacaag attgagactt tggaattacc agttattgag cgtgtcaaat tccaccgttg
    15721 gaatctgggt gatggaacga ttacagaaaa tgagccatca gcaaatgttc cagatttcac
    15781 agctttagat catcacttga agttggtgca agtaggaact caaactgttt tcgaacaaac
    15841 tccagttgag ttagctgaac agggtgttgt cttcacagac tttcactcag ctttagaaga
    15901 aattccagag ctgatcgaag aattcttcat gtcatctgtt aagtatgatg atgacaagtt
    15961 ggcggcttac cacacagctt actttaacag tggtgctgta ctctatattc cagataacgt
    16021 agaaatcaca gagccaattg aaggaatttt ctaccaagat agcgatagca atgtgccgtt
    16081 taacaagcat attatgatta tcgctggtaa aaattctaag attagttatc tggagcgttt
    16141 agagtcacgc ggtgaaggaa gtgacaaagc aactgccaat atcacagtgg aagtgattgc
    16201 acgttctggt gcgcaagtca agtttgctgc tatcgaccgt ctaggtgaaa acgtcactgc
    16261 ctacattagc cgtcgtggta aattaggcaa cgatgcaagt attgactggg ctatcggtgt
    16321 catgaacgaa ggaaatgtcg ttgctgattt tgatagtgac ttgattggta atggtagcca
    16381 tgctgacctc aaggttgtag ctctttcaag tggtcgtcag gtacaaggga ttgatactcg
    16441 tgtaactaac tatggctgca actcaatcgg aaacattcta caacatgggg ttatccttga
    16501 aaaagcaact ttgactttca atggtatcgg ccacatcatc aagggtgcta agggagcaga
    16561 tgcgcaacaa gagagccgtg ttctcatgct ttcagaccaa gcgcgttcag atgctaaccc
    16621 aattcttttg attgatgaaa atgacgtaac tgcaggccat gcagcctcta ttggtcaggt
    16681 agatccagaa gatatgtact acctcatgag tcgtggcttg gataaggcaa ctgcagagcg
    16741 tttggttgtt cgtggtttcc ttggatctgt tatcgtggag attccagtca aggaagttcg
    16801 tgatgaaatg attgcaacta tcgaagagaa attgtcaaaa cgctaagggg cagcctatgt
    16861 tagatgtaga agcgattcgc aaggattttc caattttaga tcagattgtc aatgatgaac
    16921 ctctggtcta tctggacaat gctgcgacga cacaaaaacc actagtagtt ctgaaagcta
    16981 ttaacagcta ctatgagcag gacaatgcca atgttcaccg tggtgtccat accttagcgg
    17041 aacgagcgac agcttcttat gaagctgctc gtgaaaccat tcgtaagttt attaatgcag
    17101 gctctacaaa ggaagttctc tttaccagag gaacgacaac cagccttaac tgggtggcac
    17161 gctttgctga ggaaattctc actgagggag accaggtctt gatttcagta atggaacacc
    17221 attctaatat cattccatgg caggaagctt gtcgaaagac tggagcagag cttgtctatg
    17281 tctatcttaa agacggtgcc ttggatatgg aggatttgcg agctaaattg actgataagg
    17341 ttaaatttgt ttccctagct catgcctcca atgttcttgg tgtggtcaat ccgatcaagg
    17401 aaatcactca attagcccac caagttgggg caatcatggt agtggatggt gctcaatcta
    17461 cacctcatat gaagattgat gtccaggact tggatctgga ctttttcgcc ttttcggctc
    17521 acaagatggc tggtccgact ggtatcggtg tcctttacgg caaagaaaag tatcttgagc
    17581 aaatgtctcc agtagaattt ggcggcgaga tgattgattt tgtctacgag caatctgcta
    17641 gttggaagga attgccttgg aaatttgagg ctggaacgcc aaatatggca ggagctattg
    17701 gacttgcgac tgcagttgat tatctggaaa agattggtat ggatgccatt gaagctcatg
    17761 aacaggaatt gattgcgtac gtctatccaa aactgcaggc aattgaggga ttgaccattt
    17821 acggttctca ggatttggct caacgttcgg gtgttattgc ctttaaccta ggtgatctcc
    17881 atcctcacga tcttgcgacg gctctggatt atgaaggagt ggctgttcgt gctggtcacc
    17941 attgtgcgca acccttgctt cagtatttgg aagtcccagc aacagctcgt gcaagttttt
    18001 atatctacaa taccaaggca gattgcgaca aactagtcga tgccctacaa aagacaaagg
    18061 agtttttcaa tggcactttc taaactagat agcctttata tggcagtggt agcagaccat
    18121 tcgaaaaatc cacatcacca agggaagtta gaagacgctg agcaaatcag tctcaacaat
    18181 ccgacttgtg gggatgtcat caacctctct gtcaagtttg atgcagagga ccgtttggaa
    18241 gatattgctt ttctaaattc aggatgcacg atttcaactg cttctgctag tatgatgaca
    18301 gatgccgttt taggaaaaac caaacaagaa attttagaac tggcgactat tttttctgaa
    18361 atggttcaag ggcaaaaaga tgagcgtcaa gaccaacttg gagacgcggc attcttgtca
    18421 ggtgttgcca aattccctca aagaatcaag tgtgcaaccc tagcttggaa tgcccttaag
    18481 aaaacaattg aaaatcaaga aaaacagtaa gacaagtttc ttttgtctta tgaattatta
    18541 gaaatgaaga aagaaaggat attatggctg aagaaagagt agaaccaaaa ccaattgacc
    18601 ttggtgaata taaatttggt ttccatgacg atgtagagcc tgtcttatcg acaggaaaag
    18661 gactcaacga aggtgttatt cgtgaattat ctgctgctaa gggtgagcct gagtggatgt
    18721 tggagttccg tttgaagtct tatgaaacct tcaaaaaaat gcccatgcaa acttggggag
    18781 cagacttgtc agagattgac tttgatgact taatctacta ccaaaaacca tctgacaaac
    18841 cagcccgttc ttgggatgat gtacctgaaa agattaaaga aacctttgaa cgtatcggga
    18901 ttccagaagc tgaacgtgct tatttagcag gggcttctgc ccagtacgag tcagaagtgg
    18961 tttaccacaa catgaaggaa gagttccaaa aattaggtat tatctttaca gatacagatt
    19021 ccgcactcaa ggaataccca gacttattta aacaatactt tgcgaagttg gtaccgccga
    19081 ctgataacaa gttggcagcc ctcaactcag cagtatggtc gggtggaact tttatctacg
    19141 tgccaaaagg tgtcaaggta gatattccac ttcaaactta tttccgtatc aataacgaaa
    19201 atataggtca gttcgaacgt accttgatta tcgttgatga gggagcaagc gtccactacg
    19261 tagaaggatg tacagcacca acatattcaa gcaatagctt acacgctgcc attgtagaaa
    19321 tttttgcttt ggacggagct tatatgcgtt atacaactat ccaaaactgg tctgataacg
    19381 tctataactt ggtaacaaag cgtgctaagg ctcaaaagga tgccactgtt gagtggattg
    19441 atggaaactt gggtgccaaa acgactatga aatatccatc tgtttacctt gatggagaag
    19501 gagcgcgtgg taccatgctc tctatcgcct ttgctaatgc agggcaacac caagacacgg
    19561 gtgctaagat gattcacaat gctccacata ccagctcgtc tattgtgtct aaatccatcg
    19621 ctaaaggtgg aggaaaggtt gactaccgtg gacaagtcac ctttaacaag aactctaaga
    19681 aatctgtttc ccacattgaa tgtgatacca ttatcatgga tgacttgtca gcatcagata
    19741 ctattccatt taatgaaatt cacaactcgc aagtggcttt ggaacacgaa gccaaagtat
    19801 ctaagatttc agaagagcaa ttgtattatc tcatgagccg tggattgtca gaatctgagg
    19861 caactgaaat gattgtcatg ggatttgtag aaccctttac aaaagaactt ccaatggaat
    19921 acgcagttga gctgaaccgc ttgattagct atgaaatgga gggatcagtt ggataaaatt
    19981 tgattttata ctcttcgaaa atctcttcaa accacgtcag catcgcctta tcgtatgtat
    20041 ggttactgac ttcgtcagtt tcatctacaa cctcaaaaca gtgttttgag caacctgcgg
    20101 ctagcttcct agtttgttct ttgattttga agatgaactt gtatcaaaaa atcgcggttt
    20161 aaaatcgcga ttttttataa tttctcgtta acaaagcgga caaactgatt ccaccaaact
    20221 tttaagaaga aggctttttc aattttcttg tctgctacca tttcgaaact agggcgctct
    20281 gtggtgatgt aaccttgacc aatcaagtcc ttgtcttcat aagtcaaatg gccaaccact
    20341 gttccagctt caagtggtgc tgggattgct ttggaatcag gtgtgaattg aacagattga
    20401 gaagattgat tcccaacacg ttcgattaga tagatatcct ctggagccac tgcagttact
    20461 gtatcttctt ttccatcttg tacaggggct ttgctatctt gataggcatc gccttgttga
    20521 acgattttgc gaagtgtaaa tgtagaagaa atataatcca ttagggaaga tgtagctgta
    20581 aatcgagcgt aaggattatt gtcttgatga tctgcattta aaacaactgt gatgactctc
    20641 atgccttttt cgacagtagt accaacaaaa gactctccag ccttatctgt tgttcctgtt
    20701 tttagcccat caaaaccacc acggtaagca ggcatacctt ctaacatgta gttggttgaa
    20761 gtgattgtca tcccagcaaa agtagaagaa ggttttttgg tgatttctaa gacttgtggg
    20821 tattttttga tgaggttgcg agcaacgata gcgacatcat aagcactaag cttattttcc
    20881 tcatcttttt tagaacctgg gtaaatgtta tcccctagag tttcattgtt aagacctgtc
    20941 gtattgacaa cagtggcatc ctgaattccc cattccaaga gttttgcccg catcatatcg
    21001 acgaaatctt tttctgagcc agcaattttc tcagctaggg caatagcggc gctgttggca
    21061 ctagatacca gagttgcttc aagcaactct tcgacagtat aattacgggc ctccatagga
    21121 atattactgg cttcagaatt tgtcgtcaat tgataaggat aatcagaaat atctacagga
    21181 gtggagaggg taatacttcc gttttccaaa gcttcataga ctagataaac agtaatcaat
    21241 tttgttatgg aagcaatttc gacaggttgc gttgcatcct tctcatagag aattttacca
    21301 gtatttgcct caacagcaat cgcatgttta gcggcaatgg taaaatcttg agcaacagca
    21361 gtagaaacac cccctaaaag agagacagtt aacaaagtta aaaatatttt tttcatagta
    21421 gtcttattct atcataaaga aaaaaaatat tcttgcttta ataattcatc tgttaagctt
    21481 tttgaaaata tggtaaaata aagtgaggga ggtaactcat gtttcgtaga aataaattat
    21541 ttttttggac cacagaaatt ttactcttaa ccatcatctt ttacctatgg agacagatgg
    21601 gatctttgat taaccctttt gttagcgtgc ttaatacaat tatgattcca tttttattag
    21661 ggggctttct ttattatttg acaaacccta ttgttacttt cttaaataaa gtctgtaaac
    21721 tcaatcgttt gcttggtatt ttaattacct tgtgtacttt ggtctgggga atggtcatag
    21781 gtgttgtcta tctcttacct attttgatta atcagttatc tagtttgatt atatctagtc
    21841 aaactattta tagtcgagta caagacttaa tcatagactt atctaattat cctgcgctcc
    21901 agaatttgga tgtagaagct acaattcagc agttaaactt atcctatgtt gatattcttc
    21961 aaaatatcct aaatagcgta tcaaatagtg tggggagcgt cttgtcagct cttatcagta
    22021 ctgttttgat tttgattatg actccagttt ttttggttta tttcttatta gatggacata
    22081 aattcttgcc catgcttgaa agaacgattc taaagaggga tcgcttgcat attgcaggct
    22141 tattaaagaa tttaaatgcg acgattgctc gctatattag tggagtttcg attgacgcaa
    22201 tcattatagg ttgtttggct tatattggct atagtattat tggtttaaaa tatgctttag
    22261 tttttgccat tttttctggt gtagccaatt taattcctta tgtggggcca agtattggtt
    22321 tgattcctat gatcatcgca aatatattca ctgatcccca tagactgctg attgcagtga
    22381 tttatatgct tgttgttcag caggtagatg gcaatatctt atatcctcga attgtaggaa
    22441 gtgttatgaa ggttcatcca atcacgattt tagttttact tttgttgtca agcaatatct
    22501 atggtgtagt tggaatgatt gtcgcagtgc caacctattc tatcttgaaa gaaatttcta
    22561 agttcttatc ccgtttgtat gaaaatcata aaataatgaa agaacgagaa agagaattag
    22621 ctaagtaaaa gtcaggagaa ccctgatttt tctttactgg aagtggcctt tagattagaa
    22681 gactgaaaat aagttaaagt cttaaactaa ttttcacagc taagaatagt agaagttaat
    22741 ctgataaaaa tcgaaaaaac cagtggaatt ccgtgtcagg gtaagttcca ctggttttca
    22801 tagtctatta aagttcgaat gaaacctatt tatagtatat tgaaactaga atagtacacc
    22861 tctaattcta aaacattgtt agaaatcgat ttgactgtcc tgatctattc gtcctattct
    22921 tatttcattt tactatattt tggtgcaata agtgaaaagt agtccgaata atataaggat
    22981 tgattttata gtttttaaac tcaaatgaat taaaataaag agagtacgaa aattctcatc
    23041 tgaaagtatt ttagaataat tctcttcgtg aatttcttca aaacagatag cttcatctta
    23101 ggtatgtgtt aattggattt cgtaagctct atttacaatc gtaaagttat gcactgaaaa
    23161 agttgtagtt agtcttccag tttgctaaat ttctcgaata gtatcttttt taggtatgtg
    23221 atttcttttt gcatttttga gttagataag gtataatggt tttattgtct tttggggtcg
    23281 ttacggattc gacaggcatt atgaggcata ttttgcgact cgtgtggcga cgtaaacgct
    23341 cagttaaata taactgcaaa aaataacact tcttacgctc tagctgccta aaaaccagca
    23401 ggcgtgaccc gatttggatt gctcgtgttc aatgacaggt cttattatta gcgagatacg
    23461 attaagcctt gtctagcggc ttgataagag attgatagac tcgcagtttc tagacttgag
    23521 ttatgtgtcg aggggctgtt aaaataatac ataacctatg gttgtagaca aatatgttgg
    23581 caggtgtttg gacgtgggtt cgactcccac cggctccatt attcctttgc attcttttgc
    23641 attccttggt aaaacgttgt taaatcaacg ttttttattt ttatctttgg tattcctttg
    23701 cattcttttg ctaaaaaggg agtcacaaac agaccctatt ttaaaaaaag gatagaaaaa
    23761 aggatacaac atttgtcgca tcctaaaaat aatctttttt cgacggaaga catgggattc
    23821 gaacccacgc acgctattac acgcctaccg cgtttccaac acggcctctt aagcctcttg
    23881 agtaatcttc caatacttac tcaaatagtc taccataaag gctcttatct tgcaataaaa
    23941 attctagaaa taagaaaaat gatagatttt gaaagaaaat gataaaaaat gcttgacttc
    24001 aaaagaaagt atgatagaat gaatagtgta aacgataaca ggaggtgatt cggtgttaaa
    24061 aacagagcgg aagcaactga ttttagagga gttaaatcaa catcatgtag tttctctaga
    24121 aaaattagtt agtttgctag aaacgtcaga atcaacggtt cgaagagact tggatgagtt
    24181 ggaagcggaa aacaagcttc gtcgtgtgca tggtggagca gaactccccc actccttaca
    24241 ggaagaagaa accattcaag aaaaatcggt caaaaacctt caagaaaaga agttgctggc
    24301 tcagaaagca gcctctctca ttaaagaaaa agatgtcatc tttatcgatg ctggaacaac
    24361 aactgctttt ttgattcatg aattggtcaa taagaatgtt acagttgtga ccaactccat
    24421 tcaccatgcc gctcagttgg ttgaaaagca gattccaact gtcatggttg gaggaaacgt
    24481 caagacggcg acagatgcta gtatcggggg cgttgctctt aaccagatta accaattgca
    24541 ctttgaccgt gcctttatcg gaatgaatgg tgttgacgat ggctattata cgactcctga
    24601 tatggaggag ggagctgtga aaagagctat tttggagaat gccaagcaga cctacgtctt
    24661 ggtggattcg tcaaaaattg gacaaacttg ctttgccaag gtagcaccac tcaaacgcgc
    24721 tatcgttatc actagtcaag ggcatgagct cttgcaggtt attaaggaga aaacggaggt
    24781 aatagaagta tgatttatac agtcacactc aatccatcca ttgactatat cgttcgtttg
    24841 gaccaagtca aagttggtag tgtaaatcgt atggacagtg atgataagtt tgctggtggg
    24901 aaaggaatca atgtcagccg tgtcttgaaa cgtttgaata taccaaatac agcgacggga
    24961 tttatcggtg gctttactgg taaatttatc acagatactt tagcagagga agaaatcgag
    25021 acacgttttg tccaggtggc agaagatact cgtatcaatg ttaaaatcaa agcagaccaa
    25081 gaaacagaaa tcaacggaac gggtccaact gttgaaccgg ttcagctaga agaattgaaa
    25141 gctattttat ctagtctgac agcagaagat acagttgtct ttgcaggttc aagtgctaaa
    25201 aatctaggca atgttatcta taaggatttg atttccttga cgcgccagac tggtgcgcaa
    25261 gtggtctgtg actttgaagg acagacctta attgatagtt tggactacca gcctcttctt
    25321 gtaaaaccaa acaatcatga acttggagcg atttttgggg ttaaactcga aagtttagat
    25381 gaaattgaga aatacgctcg tgagttactg gctaagggtg ctcaaaatgt tattatctct
    25441 atggctggtg atggcgccct tcttgtcaca tctgagggag cttacttcgc taaaccaatc
    25501 aaaggaacag tcaaaaattc agttggagct ggtgattcta tggttgctgg attcacaggt
    25561 gaatttgtca aatcaaaaga cgcagtagaa gccttcaaat ggggagtggc ttgcggaacg
    25621 gcaactacct tctcagatga cttggcaacg gcggaattta ttaaagaaac atatggaaaa
    25681 gttgaggtag aaaaaagatg aaaattcaag acctattgag aaaagatgtc atgttgctag
    25741 atttgcaggc aactgaaaaa acagctgtca tcgacgagat gattaaaaat ttgacagagc
    25801 acggttatgt aacagatttt gaaacattta aagaaggaat tttggcgcgt gaagctttga
    25861 cttctactgg tttgggtgat ggaatcgcaa tgcctcacag caaaaacgct gctgtcaaag
    25921 aagcgacagt tctatttgct aagtcaaata agggtgttga ctacgagagc ttggatggac
    25981 aagcaactga cctcttcttc atgattgcag ctccagaagg tgccaatgat actcacttgg
    26041 cagccttggc agaattgtct caatacttga tgaaagacgg ttttgcagac aaacttcgtc
    26101 aagcaacatc tgcagaccaa gttatcgaac tttttgacca agcttcagaa aaaactgagg
    26161 aacttgttca agcacctgct aatgactctg gtgactttat cgtagctgtt acagcttgta
    26221 caacaggtat tgcccacact tacatggccc aagaagccct tcaaaaagta gctgctgaaa
    26281 tgggggttgg tatcaaggtc gaaaccaacg gtgctagcgg tgttggaaat caactaactg
    26341 cagaagatat ccgtaaggct aaagctatta tcattgcagc agacaaggcc gttgaaatgg
    26401 atcgatttga tggaaaacca ttgatcaatc gtccagttgc tgacggtatc cgtaagacag
    26461 aagagctaat taacttggct ctttcaggag atactgaagt ctaccgtgcc gctaatggtg
    26521 ccaaagctgc aacagcctct aacgaaaaac aaagccttgg tggtgccttg tacaaacact
    26581 tgatgagtgg tgtatctcaa atgttaccat tcgttatcgg tggtggtatc atgattgccc
    26641 ttgccttctt gattgacggt gctttgggtg ttccaaatga aaaccttggc aatcttggtt
    26701 cttaccatga gttagcttct atgttcatga aaattggtgg agctgccttt ggtttgatgc
    26761 ttccagtctt tgcgggttat gttgcctact ctattgctga aaaaccgggt ttggtagcag
    26821 gtttcgtggc tggtgctatt gccaaagaag gttttgcctt tggtaaaatt ccttatgccg
    26881 caggtggtga agcaacttca actcttgcag gtgtctcatc tggtttccta ggtgcccttg
    26941 ttggtggatt tatcgcaggt gccttggttc ttgccatcaa gaaatacgtt aaagttcctc
    27001 gttcactcga aggtgctaaa tcaatccttc tattgccact tcttggaaca atcttgacag
    27061 gatttgttat gctagctgtg aatatcccaa tggctgcaat caacactgct atgaatgact
    27121 tcctaggcgg tcttggagga ggttcagctg tccttcttgg tatcgtcctt ggtggaatga
    27181 tggctgttga catgggtgga ccagttaata aagcagctta tgtctttggt acaggtacgc
    27241 ttgcagcaac tgtttcttca ggtggttctg tagccatggc agcagttatg gctggaggaa
    27301 tggtgccacc acttgcaatc tttgtcgcaa ctcttctttt caaagataaa tttactaagg
    27361 aagaacgtaa ctctggtttg acaaacatca tcatgggctt gtcatttatc actgagggag
    27421 cgattccatt tggtgccgct gacccagctc gtgcgattcc aagcttcatc cttggttcag
    27481 cagtagcagg tggactcgtt ggtcttactg gtatcaaact catggcgcca cacggaggaa
    27541 tcttcgttat cgcccttact tcaaatgctc tcctttacct cgtttctgtc ttggtaggag
    27601 caatcgtaag tggtgtggtt tatggttacc tacgcaaacc acaagcataa aaaatagaaa
    27661 aatgaaaaga ttggaccgtt tggtgcagtc tttttctctt cccgaaatgc ctgtgaaata
    27721 tggtataata gaagaatggc aaacaagaat acaagtacaa caagacggag accgtctaaa
    27781 gcagaactgg aaagaaaaga agcgattcaa cgaatgttga tttcgttagg aattgcgatt
    27841 ttattgattt tcgcagcctt caaattaggg gctgcaggta taacccttta taatttaatt
    27901 cgcttgctag tgggtagcct agcttatctg gcgatattcg gcctattaat ctatctcttc
    27961 tttttcaagt ggatacgaaa acaggaagga ctcttatctg gctttttcac catatttgct
    28021 ggcttactct tgatttttga ggcctacttg gtttggaaat atggtttgga caagtccgtt
    28081 ctaaaaggga ccatggctca ggttgtgaca gatctgactg gttttcgaac gactagcttt
    28141 gctggagggg gcttgatcgg ggtcgctctt tatattccaa cagcctttct cttttcaaat
    28201 atcggaactt actttattgg ttctatcttg attttagtgg gttctctcct agtcagccct
    28261 tggtctgttt acgatattgc tgaatttttc agtagaggct ttgccaaatg gtgggaaggg
    28321 cacgagcgtc gaaaagagga acgctttgtc aaacaagaag aaaaagctcg ccaaaaggct
    28381 gagaaagagg ctagattaga acaagaagag actgaaaaag ccttactcga tttgcctcct
    28441 gttgatatgg aaacgggtga aattctgaca gaggaagctg ttcaaaatct tccacctatt
    28501 ccagaagaaa agtgggtgga accagaaatc atcctgcctc aagctgaact taaattccct
    28561 gaacaggaag atgactcaga tgacgaagat gttcaggtcg atttttcagc caaagaagcc
    28621 cttgaataca aacttccaag cttacaactc tttgcaccag ataaaccaaa agatcagtct
    28681 aaagagaaga aaattgtcag agaaaatatc aaaatcttag aagcaacctt tgctagcttt
    28741 ggtattaagg taacagttga acgggccgaa attgggccat cagtgaccaa gtatgaagtc
    28801 aagccggctg ttggtgtaag ggtcaaccgc atttccaatc tatcagatga cctcgctcta
    28861 gccttggctg ccaaagatgt ccggattgaa gcaccaatcc ctgggaaatc cctaatcgga
    28921 attgaagtgc ccaactccga tattgccact gtatctttcc gagaactatg ggaacaatcg
    28981 caaacgaaag cagaaaattt cttggaaatt cctttaggga aggctgttaa tggaaccgca
    29041 agagcttttg acctttctaa aatgccccac ttgctagttg caggttcaac gggttcaggg
    29101 aagtcagtag cagttaacgg cattattgct agcattctca tgaaggcgag accagatcaa
    29161 gttaaattta tgatggtcga tcccaagatg gttgagttat ctgtttacaa tgatattccc
    29221 cacctcttga ttccagtcgt gaccaatcca cgcaaagcca gcaaggctct gcaaaaggtt
    29281 gtggatgaaa tggaaaaccg ttatgaactc tttgccaagg tgggagttcg gaatattgca
    29341 ggttttaatg ccaaggtaga agagttcaat tcccagtctg agtacaagca aattccgcta
    29401 ccattcattg tcgtgattgt ggatgagttg gctgacctca tgatggtggc cagcaaggaa
    29461 gtggaagatg ctatcatccg tcttgggcag aaggcgcgtg ctgcaggtat ccacatgatt
    29521 cttgcaactc agcgtccatc tgttgatgtc atctctggtt tgattaaggc caatgttcca
    29581 tctcgtgtag catttgcggt ttcatcagga acagactccc gtacgatttt ggatgaaaat
    29641 ggagcagaaa aacttcttgg tcgaggagac atgctcttta aaccgattga tgaaaatcat
    29701 ccagttcgtc tccaaggctc ctttatctcg gatgacgatg ttgagcgcat tgtgaacttc
    29761 atcaagactc aggcagatgc agactacgat gagagttttg atccaggtga ggtttctgaa
    29821 aatgaaggag aattttcgga tggagatgct ggtggtgatc cgctttttga agaagctaag
    29881 tctttggtta tcgaaacaca gaaagccagt gcgtctatga ttcagcgtcg tttatcagtt
    29941 ggatttaacc gtgcgacccg tctcatggaa gaactggaga tagcaggtgt catcggtcca
    30001 gctgaaggta ccaaacctcg aaaagtgtta caacaataaa aaaatagctt ctttccaagt
    30061 ttggagggaa gctatttttc tggctattga ttgcttttat tttctgaagt tggcgcattg
    30121 gactgttttt cgttttcagt agcaggttta cttgaagcag gagtagaaga gtcctgagtt
    30181 gctgttttct gatcttcttt tttctcttcc ttgacgctag attttggtgt ttcctcttgc
    30241 tgtgtttttt cttgactagt gttagtctct ttagttggac tggtgttttc cttaggggat
    30301 tccttttgga tttctttgac aatggttgtc gtctggcttg tcgtaggttc ttttttaata
    30361 tttttgttat tatccaaggc gttcataatc gtgatacttg cggtaatcaa gccaaggata
    30421 ctagcgattg tagcaacggt tttttgaaag actgtaaagc cttttttgat gtgttctgtt
    30481 tttggttttg aagttctacg atagttagac atacattctc tcctttgatt atgatttatt
    30541 atactctgtt tctttaaaaa aatcttaaaa aaagagaaat ttccctttct tgtcgcaagc
    30601 tccgtttcat gatacaatag aggaagtgat tttagaaagc gtgagaaaca tgatttactt
    30661 tgataattca gcgacgacca agccttatcc tgaagccctt gaaacctata tgcaggtcgc
    30721 ttcaaaaatt ttaggaaacc catctagtct ccatcgtttg ggagaccaag caacacgaat
    30781 cttagatgct tctcgccaac agattgcaga tttaatcggt aagaaaagcg atgaaatctt
    30841 ctttacctcg ggtggaacag aaggggataa ctggcttatc aagggtgtgg cctttgaaaa
    30901 agctcagttt ggcaagcaca tcattgtttc agccattgaa catccagcag tcaaagagtc
    30961 agccctctgg ttgaaaagtc aaggatttga agtggatttt gctccagttg ataagaaagg
    31021 cttggtcgat gttgaggcgt tagcaggttt gatacggcat gatacaatcc tcgtttccat
    31081 catggctgtg aacaatgaaa tcggctctat ccaacctatt gaggctattt cagaattctt
    31141 ggcagacaag ccgactattt ccttccacgt tgatgcggtt caggcgcttg ccaaaattcc
    31201 gactgaaaag tatctgacag aacgggtgga ttgcgcgact ttctctagtc acaagttcca
    31261 cggggttcga ggtgttggct ttgtctatat caaatctggc aagaagatta cacctcttct
    31321 tacaggtggt ggccaggagc gagattatcg ttcgacaact gaaaatgtgg cagggattgc
    31381 agcgacagcc aaggccctcc gtttgtctat ggaaaagcta gatatcttta ggagcaagac
    31441 tgggcagatg aaggcagtga ttcgccaagc tcttctgaac tatccggata tttttgtctt
    31501 ttcagatgag gaaaactttg cacctcatat tctgactttt ggaatcaaag gtgttcgagg
    31561 tgaagtcatc gttcacgcct ttgaagacta tgatattttc atctcaacaa cctcagcttg
    31621 ttcatctaag gcaggaaaac cagccggtac cttgattgcc atgggagtgg acaaagataa
    31681 ggccaagtca gctgtgcgtc ttagcctaga cttggaaaat gatatgagtc aggtcgagca
    31741 gtttttgacc aagttaaaat tgatttacaa tcaaactaga aaagtaagat aggagcattc
    31801 atgcagtatt cagaaattat gattcgctac ggagagttgt caaccaaggg taaaaaccgt
    31861 atgcgtttca tcaataaact tcgtaataat atttcggacg ttttgtctat ctatacccaa
    31921 gttaaggtaa cagcagatcg cgaccgtgcc cacgcttacc tcaatggagc tgattacaca
    31981 gcagttgcag aatctctcaa acaagttttt ggaattcaaa acttttctcc tgtttataag
    32041 gttgaaaaat ctgtagaagt tttgaagtct tctgtccaag agattatgcg ggacatctac
    32101 aaggaaggta tgacctttaa gatttctagc aagcgtagcg accacaactt tgaacttgat
    32161 agtcgtgaac tcaaccaaac acttggaggg gctgtattcg aagccattcc aaatgtgcaa
    32221 gttcaaatga aaagtcctga catcaatctt caggtggaga ttcgtgaaga agcagcctat
    32281 ctttcttatg aaaccattcg tggggctggt ggtttgccag ttggaacttc aggtaaaggg
    32341 atgctcatgt tgtcaggagg gattgactca cctgtagcag gttatcttgc tcttaagcgt
    32401 ggggtggata tcgaggcagt tcactttgct agtccaccat atactagtcc tggtgccctc
    32461 aagaaagcgc aggacttgac tcgtaaattg accaagtttg gcggaaatat ccagtttatc
    32521 gaggtgcctt tcacagagat tcaagaggaa atcaaagcca aagcgccaga agcttatttg
    32581 atgactctaa ctcgtcgctt tatgatgcgg attactgacc gtattcgtga ggtacgaaat
    32641 ggtttggtta tcatcaatgg ggaaagtcta ggtcaagtag ccagccaaac ccttgaaagt
    32701 atgaaggcta tcaatgctgt taccaacact cccatcattc gtcctgtggt taccatggac
    32761 aagttggaaa tcattgacat cgcccaggaa atcgatacct ttgacatttc aatccaaccg
    32821 tttgaagact gttgtaccat ttttgcacca gatcgtccaa aaacaaatcc taaaattaag
    32881 aatgcggagc agtacgaagc gcgtatggat gttgaaggct tggttgagcg agcagtggct
    32941 ggaatcatga ttactgaaat cacacctcaa gccgaaaaag atgaagttga tgacttgatt
    33001 gacaatctgc tctaattcag aaaatccaaa agaatagcga aaatcagtaa aaaaagttag
    33061 ttttttctct aaaaacaggt aaaaaactaa ctttttttat ttttatgata taatgatata
    33121 aaattttgaa tatagagagt tttctgacaa tgaatcaatc ctacttttat ctaaaaatga
    33181 aagaacacaa actcaaggtt ccttatacag gtaaggagcg ccgtgtacgt attcttcttc
    33241 ctaaagatta tgagaaagat acagaccgtt cctatcctgt tgtatacttt catgacgggc
    33301 aaaatgtttt taatagcaaa gagtctttca ttggacattc atggaagatt atcccagcta
    33361 tcaaacgaaa tccggatatc agtcgcatga ttgtcgttgc tattgacaat gatggtatgg
    33421 ggcggatgaa tgagtatgcg gcttggaagt tccaagaatc tcctatccca gggcagcagt
    33481 ttggtggtaa gggtgtggag tatgctgagt ttgtcatgga ggtggtcaag ccttttatcg
    33541 atgagaccta tcgtacaaaa gcagactgcc agcatacggc tatgattggt tcctcactag
    33601 gaggcaatat tacccagttt atcggtttgg aataccaaga ccaaattggt tgcttgggcg
    33661 ttttttcatc tgcaaactgg ctccaccaag aagcctttaa ccgctatttc gagtgccaga
    33721 aactatcgcc tgaccagcgc atcttcatct atgtaggaac agaagaagca gatgatacag
    33781 acaagacctt gatggatggc aatatcaaac aagcctatat cgactcgtcg ctttgctatt
    33841 accatgattt gatagcaggg ggagtacatc tggataatct tgtgctaaaa gttcagtctg
    33901 gtgccatcca tagtgaaatc ccttggtcag aaaatctacc agattgtctg agattttttg
    33961 cagaaaaatg gtaagttaag aaaggaaaaa acgaaatgca tattgaacat cttagccact
    34021 ggagtggtca tcttaaccgt gaaatgtacc ttaaccgtta tggacatggt gggattccag
    34081 ttgtggtctt tgcttcatca ggtggtagtc acaacgaata ctatgatttt ggcatgattg
    34141 atgcctgtgc ttcctttatc gaggaaggcc ttgtccagtt ctttacccta tctagtttgg
    34201 atagtgagag ctggttggct acttggaaaa atgctcatga ccaagcggaa atgcaccgtg
    34261 cctacgaacg ttatgtgatt gaggaggcca ttcttttatc aagcacaaga caggttggtt
    34321 tgatggcatg atgacgacag gttgctctat gggagcctat catgcactca atttcttcct
    34381 ccagcatcca gatgtcttta ccaaagtgat tgctctcagt ggtgtttacg acgcacgttt
    34441 ctttgtcggt gattactaca acgatgatgc tatttaccaa aactcgccag tagattatat
    34501 ttggaaccaa aacgacggct ggtttattga ccgttaccgt caggcagaga ttgtgctgtg
    34561 tacggggctt ggagcctggg aacaagatgg tttgccatcc ttttacaagc tcaaagaagc
    34621 ctttgacaag aaacaaattc cagcctggtt tgctgaatgg ggacatgatg tcgcccatga
    34681 ctgggaatgg tggcgtaaac aaatgcctta tttcctcggt aatctctatt tataaaagga
    34741 gttacctatg aattaccttg ttatttctcc ctactatcca caaaactttc aacagtttac
    34801 catcgaacta gctaataaag gcatcacagt cttgggaatt ggtcaagagt cttacgagca
    34861 attggatgag cccttgcgca atagcttgac cgagtatttt cgtgttgata atcttgagaa
    34921 catagatgaa gtcaaacgtg cagttgcttt tctcttttat aaacatggtc caattggccg
    34981 catcgagtct cacaatgaat actggcttga gctagacgca acactcagag aacaattcaa
    35041 tgtttttggt gccaaaccag aggatctcaa aaagacgaaa tataagtctg aaatgaagaa
    35101 acttttcaaa aaagcaggtg ttcctgtggt acctggagct gttatcaaga cggaagcaga
    35161 tgttgatcaa gcagtgaaag aaatcggtct tccaatgatt gccaaacctg ataatggagt
    35221 gggagcagcc gcaaccttta aacttgagac agaagacgat atcaatcact tcaagcaaga
    35281 atgggaccat tcaacccttt atttctttga aaaatttgtc acttccagcg aaatctgtac
    35341 ctttgacggg ctcgtggaca aggatggaaa gattgtcttc tcaacaacct ttgactacgc
    35401 ctatacaccg cttgacctca tgatttataa gatggacaat tcttattatg tgctcaagga
    35461 tatggatcct aaactgcgca agtatgggga agcaattgtc aaagaatttg gtatgaaaga
    35521 acggtttttc catattgagt tcttccgtga gggggacgat tatattacca tcgagtacaa
    35581 taaccgccct gcaggtggtt ttaccattga tgtttataac tttgctcatt ccttggacct
    35641 ttatcgtggc tatgcagcta ttgtcgcagg agaggagttc ccggcgtcag actttgaaac
    35701 tcagtattgt ttggctactt ctcgccgtgc aaatgctcac tatgtttatt cagaagagga
    35761 tttgcttgcc aaatatagcc agcagttcaa ggttaaaaaa gtcatgccag ctgccttcgc
    35821 ggaacttcaa ggagattacc tgtatatgct gaccactccg agtcgacaag aaatggagca
    35881 gatgattgca gatttcggac aacgtcaaga ataagaacta tcggattaag gaaattaact
    35941 cccttaatcc ttttgttttg tctgataaaa aataagagca tcccaacaag gtagctatca
    36001 taaaacttgt tcgataacta tttgaagcag gattaggtgg tcagaaatta aattttaata
    36061 tttcaattga gtcatagtat tgtgtttgcg tatccttaaa tcagctaaaa ggatccatga
    36121 cgacacctat acgatatagt tttcaagata ccaaacaagt ctattaatat tcaatgaaaa
    36181 tcaaagagca aactaggaag ctagccgcag gtttctcaaa acactgtttt gaggttgtgg
    36241 atagaactga cagagtcagt atcatatacc tacggcaagg tgaagctgac gtggtttgaa
    36301 gagattttcg aagagtataa aatattcagg tgacgcatag atatagttaa ttgaagcttt
    36361 gtttgaaatc tgataaaata atgatattac taagttttaa aaactaaaga aaagggaaga
    36421 tatgattaca ggcgaattaa aaaataaaat cgatcagctg tgggaaattc tttggacaga
    36481 aggaaacgca aatcctttaa caaatattga acagttgact tatctcttat ttatgaaaga
    36541 tttggatagt gtcgagcttg gacgtgaaag tgatgctgaa tttctaggga ttccttatga
    36601 gggagttttt ccaaaagata aacctgaata ccgttggtca acttttaaaa atataggaga
    36661 tgctcaggaa gtttatcgtt taatgactca ggagattttt ccgtttatta aaaatctcaa
    36721 gggggataca gatgatacag ccttttcacg atatatgcga gaagctattt ttcaaataaa
    36781 taaacctgct acgcttcaaa aggcaatttc tatcttagat gtttttccaa ctaggggatt
    36841 agatgtagat tttgataatg acaaacaaag tattactgat atcggagata tctatgaata
    36901 tctgttatca aaattgtcga ccgcaggtaa aaatggacag ttccgtacac ctcgtcacat
    36961 catcgatatg atggttgagt tgatgcaacc gactatcaaa gatatcatct cagatcccgc
    37021 tatgggttct gctggcttct tagtatctgc tagccgttac ttaaagcgta agaaagatga
    37081 atgggaaacc aatacagata atatcaatca ttttcataat cagatgtttc atggaaatga
    37141 tacggatacg actatgttga gacttggggc gatgaacatg atgctacatg gagtagaaaa
    37201 tccacaaatc agttaccttg actcgctgtc tcaagataat gaagaagccg ataaatatac
    37261 tttggtttta gcaaatcctc cttttaaggg ctcacttgac tacaattcaa cctctaatga
    37321 ccttcttgca accgtaaaaa ccaaaaaaac agaattactc tttctttctc ttttcttgcg
    37381 aactttaaaa ccaggtggac gagcagcagt tatcgtacct gatggtgtcc tttttggttc
    37441 gtctaaagct cataaaggaa ttcgtcagga aattgtagat aatcataagc ttgatgctgt
    37501 aatctcaatg cctagtggtg tgttcaagcc ttatgctgga gtttcaactg ccattctcat
    37561 ctttacaaaa actggtaatg gtggtactga caaagtctgg ttttacgata tgaaagcgga
    37621 tggtttaagt ttggatgata agcgacaacc gattagcgac aatgatattc cagatattat
    37681 cgaacgcttt catcatcttg aaaaagaagc agaacgtcag agaacggatc aatctttctt
    37741 tgttccagtt gctgagataa aggaaaatga ttatgatttg tctatcaata aatataaaga
    37801 gattgagtat gaaaaagttg agtatgaacc aacagaggtc atcttaaaga aaatcaatga
    37861 tttagaaaaa gaaattcaag ctggcttggc tgaattagaa aaattactca agtagagagg
    37921 tggctgtatg aaaaaagtga agttggggga agtcttatct ctaaaaaaag gcaagaaagc
    37981 cactgtactt gctgaacaaa caactctaag ccaacgttat attcaaatag atgatttaag
    38041 aaataataat aatttaaaat tcactgaaag tttaaatatg actgaagcac tcccagatga
    38101 tattctgata gcatgggatg gagctaatgc aggaacagtt ggttatggat tatcgggagc
    38161 tgttggtagt acaattacgg tcttaaaaaa gaatgagcga tacaaagaaa aaattatatc
    38221 agattacttg ggagtctttt tggaaagtaa atcagaattt gcttgtgaga tagctataaa
    38281 agtgtggaga aatagcttga aatttagtat aatatagcta aactatttgt ttaaagtgag
    38341 aaaaaaatgg gaaattttag ctttctttta aaaaatgacg aatatgaatc tttttcaaaa
    38401 ccttgcattg aagctgaaaa tatgattgct acatcaactg tggctactgc ctttatggcg
    38461 cgtcgtgctt tggagcaggc tgtccattgg atatatagtc acgattcata tttagaagct
    38521 ccctatcgtg ctactctatc ttctttagta tgggatgatg attttaggga tatcgtagat
    38581 tctgaactcc acaagcagat agttctgttg attcggtggg gaaaccatgc tgctcatggt
    38641 ggtgaaatta aggaacgaga agcgatttta gctttacatc atttgtatca gtttgttaat
    38701 tttatcgatt attgttacag caatgaattt gtggagcgtt attttgatga gaagtgctta
    38761 ccactttcag caaacatcaa ataccgagaa actccacaat ctatgataaa gttacaagac
    38821 agtttaccag aactgcctga ttttcatgaa cagatggctg ctcagtccgt agaagttcaa
    38881 gagacttata ctgaaaaacg tgagactgca gcgcaacggc aagatgtgcc tttccatatt
    38941 gaacaattat ctgaggcaga gacaagaaag ctctttattg atatcgatct ccgtttagca
    39001 ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg ttgatggtct caagcacggt
    39061 tcaggaattg gttactgtga ctatgtactt tatggtaaaa atgggaaaat tttagcgatt
    39121 gtagaggcta aaaaagcctc tgtcaatcca gaagtagggg aagtacaggt caaagaatat
    39181 gctgaagctc ttgagaaaca tatcggctat cagccaattt gctttattac aaatgggttg
    39241 aagcactata tacttgatgg tccgaaccga cgccagattg caggctttta ctctcaagaa
    39301 gaattgcaat tagtgatgga tagacgtcat cttcaaaaac cacttgagga tatttctagt
    39361 aaaattagag atgatatttc cgggcgtcac taccaaaaac atgcgattgc aagcgtttgt
    39421 gaagctttct ctgatcatcg tagacaggca cttttggtta tggcaactgg ggctgggaaa
    39481 actcgtacag cagtttctct agttgatatc ttatcacgtc ataactgggt aaaaaacgtt
    39541 ctcttcttag ccgatagaac ttccttggtt aagcaagcat atgattcgtt tagaaaatta
    39601 ctcccagatc tttccgtttg taacttctta gaagataaag aaggagctca atcaagtcgc
    39661 atggtctttt caacttatcc gaccatgatt ggagcgatta gtggtcaaga agaagtaaat
    39721 caacgccctt tcactgttgg gcattttgac cttatcataa ttgacgaatc tcaccgttct
    39781 atttatcaga aatacaagtc catttttgat tattttgatg caaggattgt aggcttaaca
    39841 gctactccgc gtcaagattt agataaaaac acctatggat tctttaattt ggagaatggg
    39901 gttccaacat atgcatatga tttggaagag gctgttaaag acggatattt agtagcctat
    39961 cattctatcg aaaccaaact gaaactacct acggatggtc tacattatga tgatttgtcc
    40021 aaagaagaaa aggaacattt tgatagcaaa tttgaagacg atagctgtga aaaagatatt
    40081 gatgggagtg tatttaattc ctttattttc aataaaagta cagtagaaat tgttttaaat
    40141 gaactcatga caagaggaat tcagacagcc tcgggtgatg aaattggtaa aactattatt
    40201 tttgctaaaa atcatgatca tgcggaatat atcagaggta tttttaacaa ccgctatcct
    40261 gaaaaaggga gcgactatgc tcaggtaatt gattatagta ttaagcatta tcagaccttg
    40321 attgatgatt ttaaaattaa ggagaagtat cctcaaattg cgatttctgt agatatgtta
    40381 gatacaggta ttgatgtacc agaggttgtt aatttagtct tcttcaagaa agtacgctct
    40441 aaaactaagt tttggcagat gattggtcga ggaacccgtc tgtgtaaaga tttatttgga
    40501 cctgagcagg ataaggaaaa cttcttggta tttgattatg gggacaattt tgattatttt
    40561 cgtgcagatc caagagatgg agagggtcgt cacattgttt cgttgactca gcgtttattt
    40621 aatatcaaag tggacttgat tcgagaactt cagggactcc aataccaaga agatcagttt
    40681 gcgagagcat accgtcagca gcttgtctcg gaacttcaag gtcgtataga gagcttaaat
    40741 gagttggact tcagggttcg tatggtttta gatacagttt atagctatag gaaattggaa
    40801 agttggcaga atctaactgc tgttacaagt gaaaccattc aaaaaaatct ctctccgctt
    40861 ttatttgatg aagataaaga agatgagatg gcgaggagat ttgatttgtg gttgcttcat
    40921 attcagttgg ggcaactgac agctaaatct tccactgttc atatttccca agtgatgaag
    40981 acggctagag ctctttctgc tattggcaat atcccgcagg tttttgagca ggctgaaatt
    41041 atcaggaaag tacaggagcc tgaattttgg aaagaagtta acttgtctga tttggaaaaa
    41101 attcgtcttg ctattcgaga tttattacag tttttggata aaacagaccg taaaccctac
    41161 tatgttaact ttgaagatcg tatactctcc actgttcacg agaccacagc atttttgcag
    41221 gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt atttgaaaac tcatctggat
    41281 gaggagtcca tttctaagct ataccataat aaaaagttga catctgatga tatgcttgca
    41341 cttgaaaaat tgctctggga aaaattaggt agtaaagcag actaccaaag tcattatgaa
    41401 aataaggcaa ttccgagatt ggttcgtgag attattggct tagatagaga gtctgccaat
    41461 cgtatttttt ctaaattttt gtcggatgag aatcttaatg ccaggcagat ttcatttgta
    41521 aaattgattg tagactacat tgtagaaaat ggttttttag agacgaaagt gttaacgcaa
    41581 gagccgttta aatcttatgg ttctgttcaa ctactcttcc aacaccaact accagtactt
    41641 cgtaatattg ttcaaatcat tgaacttatc aataatcgag ctggagaagc ggcttaaatt
    41701 ctaaagtgat tgccatgctg agactcattt aaaattaaaa agagtagaaa tttatgctat
    41761 atatgagaag ttttattagg aagaatgtca tcgttttcct agaatacagt atcagttgtt
    41821 aagtggctga taaatttcaa agtagatact tgtaccacga tgtttgttga tcgagttatt
    41881 aacaaaagag ctactttgat tttaaagaaa tagaaaacaa aaagccgagc aagaattcaa
    41941 ttgcaggaga aaatgaaata atactcaatg aaaatcaaag agcaaactag gaaattagct
    42001 gcaggctgct caaaacactg ttttgaggtt gtagataa
//
LOCUS       DAVBDG010000012        40590 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBDG010000012 DAVBDG010000000
VERSION     DAVBDG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.012"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(215..442)
                     /locus_tag="VQ526_000571"
     CDS             complement(215..442)
                     /locus_tag="VQ526_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554327.1"
                     /translation="MAVVSLENNIKLYSSELFQALLKASNYKLDERIAQTVAEGYARN
                     LDYSDPELMHVGVTSVANNLLTKIKQEYFIV"
     gene            complement(433..1065)
                     /locus_tag="VQ526_000572"
     CDS             complement(433..1065)
                     /locus_tag="VQ526_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HET2554328.1"
                     /translation="MELTTKQEKQLGQTQWFHATLLRHLESLKKGIDVKFNLGSELDF
                     GPGFYITPDFEQARKFINKQVEVLNRSTSNNNIFDSEEEVGIIVEFRISNFIEIFKNP
                     DYHCHYFAKHKKSESDLDFAEFVVQNRENPDELQHHFDFIYGVQTDDNPTQALARFRQ
                     NEITKEEMLAEFRKPYSFKQLSIHNQSFCDIMKIEKVYQSKTGEELQKWQ"
     gene            complement(1147..1422)
                     /locus_tag="VQ526_000573"
     CDS             complement(1147..1422)
                     /locus_tag="VQ526_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554329.1"
                     /translation="MFTTFFKKNHDNSDVFKKLIHRLSDMSVQDLEKIDRLLDIIFTP
                     DQETEQLKTEATYREETLDDTLKEAKNQLHKEQLEKNLERFRKNGMK"
     gene            complement(1461..1844)
                     /locus_tag="VQ526_000574"
     CDS             complement(1461..1844)
                     /locus_tag="VQ526_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HET2554330.1"
                     /translation="MTKDWNFNQPLESKSENQEDPDKIAALFGNHQGGNDVNYEAAFQ
                     KRKQAPVTESNSSSKPKVTEVRTGKETDITTSYQQHLKRLIADNNSDIQSSQKKIEEL
                     HTLIDTKNKENKKLQSIYDAISELH"
     gene            complement(1841..2074)
                     /locus_tag="VQ526_000575"
     CDS             complement(1841..2074)
                     /locus_tag="VQ526_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554331.1"
                     /translation="MTIIERLEEKVTRQESKVARETEKLAAYKEQLETAMFATFKRRQ
                     SISHMSFEEALDHAFGKERQFDDSEFRKDEMSE"
     gene            complement(2125..3210)
                     /locus_tag="VQ526_000576"
     CDS             complement(2125..3210)
                     /locus_tag="VQ526_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET2554332.1"
                     /translation="MNIEECKQISILDVANRLGISFKQVSSRVYEHPEHDSFRIFSTT
                     NTFKWFSRDIQGDVIDFVRLVKGISFKEALAFLSEEPFQKEAVQEKRERPFYYPLKRV
                     EDSNCSLARYYLTKCRGISEEIIEKMIQQGLIAQASWKTNETVEPVIVFKSFDHRHKL
                     QAASIQGIYKNHSLPRERLKTILKGSHGHVGISFDIGKPNRLVFCESFIDLMSYYELH
                     QQSLSDVRLVSMEGLKRSVVAYQTLRLIAEENQKLEFLDTVIPSKLLPLFNTIRDTTS
                     YFDNHSDLLTLAVDCDDAGKDFSDKLSKSGFPVLLDLPDNEYGKEKRDWNDILREKKS
                     DLQLMFENAKETLRNQPVRQTSQCLEL"
     gene            complement(3220..3861)
                     /locus_tag="VQ526_000577"
     CDS             complement(3220..3861)
                     /locus_tag="VQ526_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2554333.1"
                     /translation="MLNKVKTKALISVGAVAATSFILMMGYTAGQHSTAKQSRKEIEL
                     AAAKLVEDKQAEDKASILSSDTVKEFLTQYYTKEKLGENNTRIQPYMTESAYSQELSS
                     QNDAMNQVYKDYILDYHFEKADIFVNQTTNQAIAMVSYNVTYVSDLKNANQSKTNQTE
                     TRTVKLSYSKLPGKLLVNQVQVWKSGLDDLDKATPKTLEESSSIPSLPNTTTK"
     gene            4021..4218
                     /locus_tag="VQ526_000578"
     CDS             4021..4218
                     /locus_tag="VQ526_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554334.1"
                     /translation="MNKLKEENLRRALSHIERHKQAINTSNNSEDNDFHKLLLQFSYE
                     VYERIKANKKPYPNLDSDKVF"
     gene            complement(4232..4528)
                     /locus_tag="VQ526_000579"
     CDS             complement(4232..4528)
                     /locus_tag="VQ526_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554335.1"
                     /translation="MNDLLLIPVIFLAVGGILILLWRLFLIASGLFLIGFISFLIFVE
                     VYGIYLFFTEPSLYFDDIRQHGLTSFTAVYLFINLMLVLGFSWHFINSINKEKI"
     gene            complement(4542..4826)
                     /locus_tag="VQ526_000580"
     CDS             complement(4542..4826)
                     /locus_tag="VQ526_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554336.1"
                     /translation="MMEDTYYQLEEALVQGFQTPEEYQAYKELKEHYEEVTGDYSFSK
                     RELTSQLEIALQNHRGVDFEEYEKEEYLDLVQKLEEFDSSLATHYRQLID"
     gene            complement(4901..11119)
                     /locus_tag="VQ526_000581"
     CDS             complement(4901..11119)
                     /locus_tag="VQ526_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET2554337.1"
                     /translation="MEVMQLLAMFRGTIPKDREKMDLFLRYQAQHFDEKWQDLVESFL
                     AEEGKIEEIPHVYSFHQDIVSFLEASSENNDQDLESYTRNFGQAGLGKLSQLSNFEKN
                     LVLEVATYNLSTRFYIQSEKEKLTPLSELVCHQNQEVNLVNVYRVANNLSDRISRDIE
                     EFLLMVDSKELEKEVLEIHFEEKEGDVLAYLGSELMATLDTVTDLVHHEGNYQQLPLT
                     QKLKIITHFDDVKARSEKSKQVEEAVLPLDNIDQVEENVEVHSLSKVDKIVEEALREY
                     PIGSQVSYKGQVFQLVSIENAQLNDLVRLELFNDSNQLFEENPILYLNSLEEIEQVLS
                     LVELEKEDSEIEIDSSSESQEIDLFSYLEEENEKDKETETLISSIEELDIPVQDFVFP
                     DDLEDFYPKTNREKIETNIAAIDLVKRLEKEGRQANPEEQELLAKYVGWGGLANEFFD
                     ELNPKYEAERLTLKSLVSKSEYSTMKQSSLTAYYTDPMIIRQIWQKLLDDGFEGGRIL
                     DPSMGTGNFFAAMPRSIRDKSELYGVELDSVTGEIAKQLHPNVHIEVRGFEEVPYQNN
                     SFDLVLTNVPFGNFRIADKNYDKPYMIHDYFVKHSLDLVRDGGQVSIISSIGTMDKRT
                     DNVLQEIKTNTHFLGGVRLPDTAFKKIAGTRVTTDLLFFQKNQAKNLNEEELVFSGSI
                     PFEEDKRVWINPYFDGKYNTQVLGEYEVRNFNGGTLNVKGESETLATEIMKAFENVEA
                     PKQIDNSLKAPVFIQEEVDNSIPSRIREDLALYSFGYEGNQIYYRDTHGIRKSSKVDE
                     ISYYVDEKGDFKAWDSSLSEHKIDRFVQLHLTEEEALDVYKSEEASKRGKYKGLFKKT
                     VFYESPLSDKDISRIKGMVDLRETYQSLIEIQRHQDYSRTEFQVLLSKLNHNYDRFVS
                     QFGYLNASVNRNLFDSDDKYSLLASLEDEYIDSKDQKVKYKKSLAFEKALVRPEREIT
                     RVSTALDALNSSLSDGRGVDLDYMVSIYPEHSQAAILDELGDQVLMDPESYLRGERKY
                     LSKNQFLSGDILNKIEVVQLLVDENNQEFDWSHALDLLESVRPPRIHLADIEFKIGSR
                     WIPQSVYGKFAFECFTNREFELSSPDVEQVIEVNPVDGQVHLRTSFVYRYPSAKDSSL
                     GVSGSRYDTGRKIFENLLNSNQPTITMTVTEGEKKKTITDLEKTSVLRAKEQHLQELF
                     QEFVSRYPEVQQVIEESYNRLYNRTVSREYDGSHLVIDGLAQNISLRPHQENAIQRIV
                     EEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKPLYVVPSSLSAQFGQEIMKFFPTK
                     KVFVTTKKDFVKARRKQFVSRIITGDYDAIVIGDSQFEKIPVSKERQMNYIEDKLNEL
                     REIKTHSENKYTVKEAEQSISGLEKQLEELQRFNRDSFIDFENLGIDFLFVDEAHHFK
                     NIRPITGLGNVAGITNTTSKKNVDMEMKVRQIQEEHDFKNIVFATGTPVSNSISELYT
                     MMNYIQPDILKRYQVDYFDSWVGAFGEIQNSMELAPTGDKYQPKKRFKKFVNLPELMK
                     IYKETADIQTQDMLDLPVPEAHIIPIESELTENQKLYLEELVMRSDMVKCGTVDPSQD
                     NMLKITGEARKLAIDMRLLDSSYSLADNHKLLQVVDNVERIYREGMENKATQMIFSDI
                     GTPKKKDNGFDVYSEIKALLVDRGIPSKEIAFVHDANSDEKKNSLSRKVNAGEVRILL
                     ASTEKGGTGLNVQSKMKAVHHLDVPWRPSDIQQRNGRIIRQGNENKEVDIYHYITKGS
                     FDNYLWATQENKLRYIKQIMTSKEPIRAAEDIDEQTMTASDFKALATGNPYLKYKMEL
                     ENDLTLLENQRRAFQRSKDHYRHTISYCEENMPILEKRLSKYEGDIQQSEMSKDQAFS
                     MTVGKQAFEQRAEAGESLHRLIRHNQADSKEFRTLASYRGFDIKMLSLPTNQPLPETF
                     SVKIVGENQYSVSLDLYSPLGTIQRLQHTIDHIKEDQVKTQNLLDELQDKLTTAKVEI
                     EKNFPKEEDYQTKKAEYDVLSPLIETETDLDIIDQALRQFHEKGKERQEQLSFELD"
     gene            complement(11167..11577)
                     /locus_tag="VQ526_000582"
     CDS             complement(11167..11577)
                     /locus_tag="VQ526_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HET2554338.1"
                     /translation="MEAIYQRDSDQDGLTDAQELALGTNPLSADSDGDGRSDLVEVEE
                     GTNPLEKDLQNINQTSLTEPSSVFMEMKQKISDMMESHYKEFIQALISIETGIENQQD
                     LEDLYTYYMRTDTVSLLSSDLETSPQEVEMEIEL"
     gene            12454..12768
                     /locus_tag="VQ526_000583"
     CDS             12454..12768
                     /locus_tag="VQ526_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HET2554339.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            12784..13170
                     /locus_tag="VQ526_000584"
     CDS             12784..13170
                     /locus_tag="VQ526_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HET2554340.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            13199..14584
                     /locus_tag="VQ526_000585"
     CDS             13199..14584
                     /locus_tag="VQ526_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2554341.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            14587..14739
                     /locus_tag="VQ526_000586"
     CDS             14587..14739
                     /locus_tag="VQ526_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET2554342.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            14762..15967
                     /locus_tag="VQ526_000587"
     CDS             14762..15967
                     /locus_tag="VQ526_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HET2554343.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            16010..16231
                     /locus_tag="VQ526_000588"
     CDS             16010..16231
                     /locus_tag="VQ526_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554344.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            16348..16845
                     /locus_tag="VQ526_000589"
     CDS             16348..16845
                     /locus_tag="VQ526_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HET2554345.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            16820..17326
                     /locus_tag="VQ526_000590"
     CDS             16820..17326
                     /locus_tag="VQ526_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET2554346.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     CTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            17310..19757
                     /locus_tag="VQ526_000591"
     CDS             17310..19757
                     /locus_tag="VQ526_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2554347.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            19760..21937
                     /locus_tag="VQ526_000592"
     CDS             19760..21937
                     /locus_tag="VQ526_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2554348.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            21934..22935
                     /locus_tag="VQ526_000593"
     CDS             21934..22935
                     /locus_tag="VQ526_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HET2554349.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            22932..23864
                     /locus_tag="VQ526_000594"
     CDS             22932..23864
                     /locus_tag="VQ526_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET2554350.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            24109..24225
                     /locus_tag="VQ526_000595"
     CDS             24109..24225
                     /locus_tag="VQ526_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HET2554351.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            24241..26160
                     /gene="tet(M)"
                     /locus_tag="VQ526_000596"
     CDS             24241..26160
                     /gene="tet(M)"
                     /locus_tag="VQ526_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HET2554352.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            26279..26446
                     /locus_tag="VQ526_000597"
     CDS             26279..26446
                     /locus_tag="VQ526_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HET2554353.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(26506..26859)
                     /locus_tag="VQ526_000598"
     CDS             complement(26506..26859)
                     /locus_tag="VQ526_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2554354.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            27364..27786
                     /locus_tag="VQ526_000599"
     CDS             27364..27786
                     /locus_tag="VQ526_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2554355.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYQHRTSGLALIKKFMEEFEE"
     gene            27783..28013
                     /locus_tag="VQ526_000600"
     CDS             27783..28013
                     /locus_tag="VQ526_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2554356.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            28474..28677
                     /locus_tag="VQ526_000601"
     CDS             28474..28677
                     /locus_tag="VQ526_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HET2554357.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            28759..29976
                     /locus_tag="VQ526_000602"
     CDS             28759..29976
                     /locus_tag="VQ526_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2554358.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(30381..33194)
                     /locus_tag="VQ526_000603"
     CDS             complement(30381..33194)
                     /locus_tag="VQ526_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2554359.1"
                     /translation="MKDKREIIRARKAFRRSLKDEKKFLKQGKKEVRKQKKDSAVLDE
                     KAWKNEIKQKLENMREASKARVKQANEDYNHILQNSPPSLLNRKELRDRRLPHARKRL
                     KIAKKQFKEAKVEAKEERKESRKERKTNQKFFYGQETKVRSNFFLQGKNLEELKAKKE
                     VKAAKENLKSTKQAYKFKKISRKAKTFLYVLGREGGELASENEDLESYRTLHETIRKG
                     KRYSRLSYNLGKASVKTGQATGRFTKKRLTNTKERYHHFKDGKGWKLTKDNPSSFKNR
                     YRKLKKQGLLSVRNIYQKLKAAFSFFTFVAGNPVTWIVGGIVFLLLLMMSFFLGFSSA
                     SLIQQDEFELTKAYTHLTWEDAEHTRTNDKGITYYTKVDDVMGYMNFKFHDYELHKPV
                     HLFSSESYKDYLSTLWHDLNDGDDLKSMQDLYETPKYKLSRDDQEEIKELKEEGVYAS
                     MQELDNPFEGKSNEDSLTMTYRYGYYDLDGKPTLQEYILLEAKAHQTIVAPMDGVVSL
                     DGDDVILTNGKGENESRLTLYSIHNGRAIEGTRVLTGDIIGETPDDTGLKVSYQKYKN
                     KKEKLVYVNPQFYFPKVIQLQTTILPAIGQFGGDEFERAKHIYEFLKSQGASPQAIAA
                     ILGNWSVESSINPKRAEGDYLSPPVGATDSSWDDESWLAIGGPAIYSGAYPNILHRGL
                     GLGQWTDTADGSTRHTALLNYARTQNKKWYDLDLQLDFMLHGDSPYYQSWLKDFFKNT
                     GSAANLAQLFLTYWEGNSGDKLLERQTRATEWYYQIEKGFSQTNGGQAKSDPQSLEGV
                     RGDLYDHSVPGGGDGMAYAYGQCTWGVAARMNQLGLKLKGRNGEKISIINTMGNGQDW
                     VATASSLGGETGSTPRAGAIVSFVGGTHGTPASYGHVAFVEKVYDDGSFLVSETNYGG
                     NPNYTFRKISQADSAISFAYTTK"
     gene            complement(33206..35563)
                     /locus_tag="VQ526_000604"
     CDS             complement(33206..35563)
                     /locus_tag="VQ526_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2554360.1"
                     /translation="MKRKSNTLKKQKTSMTNKKEEVKVKKEEVLPSTANTLSYQALYQ
                     NGLMQVKEDYFSQSYLLGDVNYQTVGLEDKGAIIEKYSDLINSLDDKTNFQLTIFNKR
                     LNLEKFRQSVLYEEKEDGYDSYRKELNRMMNQNLDSGENNFSAVKLISFGRKDSNPKQ
                     AYRSLSQIGEYFKSGFSEIDARFESLAGEERVNLLADMLRGEHHLPFSYRDLTKSGQT
                     TRHFIAPNLLDFKNKNYLQINDRLLQIVYVRDYGMELGDQFIRDLMQGDLELIVSLHA
                     QSSTKADAMKKLRTKKTLMESQKIGEQQKLARTGIYLEKVGHVLESNIDEAEELLKTM
                     TETGDKLFQTVFLIGVFGQDEEELKQALDTIQQVAGSNDLMIDKLPYMQEAAFNSLLP
                     FGCDFLEGVSRSLLTSNIAVNSPWTSVDLQDRSGKYYGINQISSNIITIDRSLLNTPS
                     GLILGTSGAGKGMATKHEIITTKIKESGENTEIIIVDPEAEYSVIGRAFGGEMIDIAP
                     DSQTYLNVLDLSEENMDEDPVKVKSEFLLSFIGKLLDRKMDGREKSIIDRVTRLTYQS
                     FKEPSLEEWVFVLSQQPEEEAQNLALDMELYVEGSLDIFSHKTNIQTGSNFLIYNVKK
                     LGDELKQIALMVVFDQIWNRVVRNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRV
                     RKYGASPTGITQNVETLLLDPNGRRIIANSEFMILLKQAKNDREELVQLLGLSKELEK
                     YLVNPEKGAGLIKAGSVVVPFKNKIPQGTQLFDIMSTDPDKMASN"
     gene            complement(35514..35873)
                     /locus_tag="VQ526_000605"
     CDS             complement(35514..35873)
                     /locus_tag="VQ526_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HET2554361.1"
                     /translation="MNTRVFKDISKYQHRAWLGFTTRQIIFVLPAFIVTIIVLGLNLF
                     FWQFGDWFVYGFVFAFTIPLMLFGVYKPNDLYFEHYLKYRLHFELTVPLRTITGKKGP
                     EHEKKIKYIKETKNFND"
     gene            complement(35927..36781)
                     /locus_tag="VQ526_000606"
     CDS             complement(35927..36781)
                     /locus_tag="VQ526_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HET2554362.1"
                     /translation="MNLSLVSPFVYLASEKISAENLFEGFNVDLQSTVDLIKSLSSYN
                     PTVWTYMSSITKSVMQPLGVAILSVVLILEFSKMAKKIANSGGAMTFEALAPMLISYI
                     MVAVVITNTTVIVEAIIGIASHAIEQVASIVAHGGAKYDTISGLKGSGFIGRMIVGFF
                     ALLIWLVRIVSAAMVNLLVSIRFIQLYLMIPFAPLTIPTFLSDEWKSIGIGYLKNIMV
                     YAVQGVLIFLIVSLVPLFESAGKIAVSNGAGVLQSLAIMFGSLVQAILLIIALVGSQR
                     TARSILGM"
     gene            complement(36798..37040)
                     /locus_tag="VQ526_000607"
     CDS             complement(36798..37040)
                     /locus_tag="VQ526_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554363.1"
                     /translation="MITHFKGFVYGVDASAMFAQAMSLLQKGLIAVGAFLVVVGIVNL
                     ATNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQISI"
     gene            complement(37061..38938)
                     /locus_tag="VQ526_000608"
     CDS             complement(37061..38938)
                     /locus_tag="VQ526_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HET2554364.1"
                     /translation="MYSGKKFLLFSLLGILLGYLFHRLTLLYDSYTGNTLDKWTHLLM
                     EGQDQVLQSPWNVSFTGKSSVFFLLGFVMMLLVYLYLETGKKQYREGVEYGSARFGTL
                     KEKKLFYGKEFSHDTILAQDVRLTLLDKKPPQYDRNKNIVVIGGSGSGKTFRFVKPNL
                     IQMNSSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMKNSDGFNPFRYIETENDLN
                     RMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQK
                     EYQNLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYG
                     TENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGKEKS
                     MVYLVIPDNDSTFRFLSALFFSTVFQTLTRQADIDFKGQLPLHVRFYLDEFPNIGEIP
                     DFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLVYLGGNDEDTFK
                     FMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHECLVRIANM
                     PVFKSKKYNSTKHPNWKYLANQETDERWWNYQINPLNQRQENHLEGLRIRDLTFESSL
                     K"
     gene            complement(38938..39426)
                     /locus_tag="VQ526_000609"
     CDS             complement(38938..39426)
                     /locus_tag="VQ526_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554365.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYSIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     TKDRSILDNHLDHLLEKMVNNPEKLADFTMDKSLDDAIDEAKSQITFRQEGAVKQKEM
                     VR"
     gene            complement(39416..39715)
                     /locus_tag="VQ526_000610"
     CDS             complement(39416..39715)
                     /locus_tag="VQ526_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554366.1"
                     /translation="MKVVNLYDLKQMGNKGGYTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            complement(40049..40564)
                     /locus_tag="VQ526_000611"
     CDS             complement(40049..40564)
                     /locus_tag="VQ526_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2554367.1"
                     /translation="MFCIGEVTILQILYYGSLISFLWMYGQRKQVIKVNMKTRIKWLG
                     IGFVSLLIISLCFSLIHAQGTTNQANLIGLQHQVPWFSFLLFLINASMVEEFLYREII
                     WNLVRKLDIRVALTSVLFALAHHPGTILAWCLYVSLGMFLGLVRYKSDLWGSMGLHLV
                     WNLLVYSLLLF"
BASE COUNT        12607 a         7843 c         7338 g        12802 t
ORIGIN      
        1 gcaaattcgg gttgtgttaa ttcatgtgtt ttacgtaagg attttatgtt ctttccaatc
       61 atggcaaact cctttatttt tataattcaa ttatagcata aaaaagcata acgcaccaaa
      121 attggtgcgt tgtttgttgt ttttctagcc taaactttac ttctaaaaaa gccaccttaa
      181 agagtaattc ctttttcggt ggcttttcaa aatactatac aataaaatac tcttgtttaa
      241 tttttgtaag caggttgtta gcaaccgaag ttacaccaac atgcatcagt tcaggatcag
      301 aataatctag attacgagca tagccttctg caacagtctg agcaatacgc tcatccaatt
      361 tataattaga ggcttttaat agtgcttgaa ataattctga actatatagt ttaatattat
      421 tttccaacga tactactgcc atttttgtaa ctcctctcct gtctttgatt gataaacttt
      481 ctctattttc attatatcac aaaaagattg gttgtgaatg gataattgtt taaaagaata
      541 gggttttcta aactcagcaa gcatttcttc ttttgtaatt tcattctgac gaaaacgagc
      601 taaagcttgg gttggattgt catcagtctg aacaccatag ataaaatcaa aatggtgttg
      661 taattcatct ggattttccc gattttgaac gacaaattcc gcaaaatcta aatctgactc
      721 tgacttttta tgttttgcaa aatagtgaca atgatagtca gggttcttaa aaatttctat
      781 aaaatttgaa atacgaaatt ctactataat tcctacttct tcttctgagt caaaaatatt
      841 attattagaa gtgcttcgat ttaatacttc aacttgcttg ttaataaatt ttctagcttg
      901 ttcaaagtca ggtgtgatat agaaaccagg tccaaagtct agctcacttc ctaaattaaa
      961 tttaacatcg attccttttt ttaaggattc taaatgtctc aataatgtag catgaaacca
     1021 ctgcgtctga cctagttgct tttcctgctt ggtagtcaac tccaaaatat ttacttcctt
     1081 ctctagtcta tactaaaagt ataacacaag actttatgca agtaaaagaa aattttataa
     1141 aaccatttat ttcattccat ttttcctaaa tctctctaaa ttcttctcca attgttcttt
     1201 atgaagttgg ttcttagctt cctttaatgt gtcgtccaaa gtttcttctc tgtaagttgc
     1261 ttctgttttt agctgttcgg tttcttgatc tggagtgaaa ataatatcta gcagtcggtc
     1321 tattttttca agatcttgga cagacatatc agatagtcta tgaattagct ttttaaatac
     1381 atcactattg tcgtgatttt tcttaaaaaa tgttgtaaac atctataacc tctcaaaaaa
     1441 aagcagccta tcaggactgc ttagtgtaat tcggaaatcg catcataaat ggactgcaat
     1501 ttcttatttt ctttattctt tgtgtcaatc aacgtatgta gctcttcaat tttcttttga
     1561 ctactttgaa tatcgctatt gttatccgca ataagtcttt taagatgttg ctgataactt
     1621 gtagtgatat ctgtctcttt tcctgttcta acctctgtaa ctttgggttt agaactagaa
     1681 tttgattccg tcacaggtgc ttgtttgcgc ttttgaaaag ctgcttcata atttacatca
     1741 ttacctcctt gatgatttcc aaataaggcc gcaattttat ctggatcttc ttgattttct
     1801 gatttacttt ctaatggttg attaaaattc caatcttttg tcattcactc atttcatcct
     1861 ttctaaattc agaatcatcg aattgtcttt ctttaccaaa ggcatgatca agagcttctt
     1921 caaaactcat gtgactgatg ctttgacgcc ttttgaacgt cgcaaacatc gctgtctcca
     1981 gttgctcttt gtaagcagct agtttttctg tctctcttgc taccttactt tcttgcctag
     2041 tgaccttttc ttctaagcgt tcaataatgg tcatatacta tcctccttca ttctaatatt
     2101 agctaaaaca tcttgatttt gttatcacaa ttctaaacat tgagaggttt gtcttactgg
     2161 ttgattcctc aatgtctctt ttgcattctc gaacatcaat tgtaaatctg atttcttttc
     2221 tcgaagaata tcgttccagt ctcttttttc tttcccatat tcattatcag gtaaatccag
     2281 taaaacagga aatcctgatt tagataactt atcagaaaaa tctttacctg catcatcaca
     2341 atctactgcc agtgtcaata aatcagaatg attatcaaaa tagctggtgg tatcacgaat
     2401 tgtattgaac aaaggcaata actttgaagg tattactgta tctaaaaatt ccaacttctg
     2461 attttcttca gctatcagtc gtaaagtttg ataagcaaca acagaccttt ttaatccctc
     2521 catagatacc aaacgaacat ctgataggct ttgttgatga agttcgtaat agctcatcaa
     2581 gtcgataaac gattcacaaa agaccagtct atttggttta ccaatatcaa aggatattcc
     2641 aacatgtcca tggcttcctt ttagaatcgt ttttaacctc tctctaggaa gagagtgatt
     2701 cttataaatc ccttgtatac ttgctgcctg cagtttgtga cgatgatcaa agcttttaaa
     2761 aacaataaca ggttcaacag tttcatttgt tttccaacta gcttgtgcta tcaaaccttg
     2821 ttgaatcatc ttttctatga tttcttctga gattcctcta cattttgtta agtaatatct
     2881 ggccagactg cagttagaat cttctactct ctttaaagga taataaaatg gtctctctct
     2941 tttttcttga acagcttctt tttgaaaagg ttcttcagaa agaaaagcta gagcttcttt
     3001 aaaggaaatt cccttaacaa gtcgaacaaa atcaataaca tcaccttgaa tatctcttga
     3061 aaaccattta aaagtattgg tagttgaaaa aattcggaat gaatcgtgtt caggatgttc
     3121 atagacacgg ctagaaactt gtttaaagga aatacctaaa cgattggcta catcaagaat
     3181 tgaaatttgc ttacattcct ctatattcat aaaacatcat cattttgtcg tagtatttgg
     3241 cagtgatggt attgatgatg attcttctaa agttttagga gttgccttat ctaaatcatc
     3301 taacccagat ttccaaacct gtacttgatt gaccaataac ttacctggta gtttcgaata
     3361 agacaactta actgttcttg tttctgtctg attagtcttt gattggttgg cattctttaa
     3421 atcagataca taggttacat tataagagac catggcaatg gcttgattcg tagtctgatt
     3481 gacaaagata tctgcttttt caaaatgata atccaaaata taatccttat acacttggtt
     3541 catagcatca ttttgacttg acaattcttg agaataagcc gattcagtca tataaggttg
     3601 aatacgtgta ttattttctc cgagcttttc tttcgtatag tactgcgtca aaaattcttt
     3661 tacagtatcc gatgacaaaa tgctggcttt atcttctgct tgtttgtcct ctacaagttt
     3721 agctgcagcc aattcgattt ctttgcgact ttgtttagca gtagaatgtt gaccagcagt
     3781 atatcccatc atgagaataa agctagttgc ggctactgct ccaacactaa ttaaggcttt
     3841 agttttgact ttatttaaca tcttagacct tctttctata aaaactaggt caagaataac
     3901 aaaaagaact tgtaagtttt atcacatcac aacgaaaggt aattcctaca tttcaggcgc
     3961 caaatcgtac agttcagaga aaaatatcaa aattagctgt cttcagctta taatataaat
     4021 atgaacaaat taaaagaaga aaatttgaga agagctcttt ctcatattga acgacataaa
     4081 caagctataa atacaagtaa taatagtgag gataatgatt ttcataaatt attactccaa
     4141 tttagctatg aagtatatga aagaatcaag gcaaataaaa aaccttatcc gaatttagat
     4201 tcagataaag tcttttgatt atctaaaaag ttcatatttt ttccttattt atggagttaa
     4261 tgaagtgcca actgaatcct agaaccaaca tcagattgat gaaaaggtac acagccgtaa
     4321 aactagttaa accatgttgt ctgatatcat caaaatataa actaggttca gtaaagaaca
     4381 aataaattcc atagacctcc acgaaaataa gaaaactaat aaaaccaata aggaaaagtc
     4441 cactggcaat aagaaagagt ctccataaaa gaatgagaat tcctcctact gctaaaaaaa
     4501 ttactgggat aaggagtaaa tcattcatga ctttctcctt tctagtcaat caattgacga
     4561 taatgggtgg caagagagga atcaaactct tctaattttt gaactaagtc caaatactcc
     4621 tctttttcat attcttcaaa gtccacacct cgatgatttt gaagagctat ttccaattga
     4681 ctagtgagtt ctcgtttaga aaaactgtaa tccccagtaa cttcctcata atgttccttt
     4741 agctccttgt aggcttggta ttcttcaggc gtttgaaatc cctgtaccaa ggcctcttct
     4801 aattgataat aggtatcttc catcataatc gttacctcgt ttctttctat ctttatttta
     4861 ccgcgctttt cttttcttgt ccgctatttg tatctatttt ttaatctaat tcaaaagaaa
     4921 gttgttcttg cctttctttt cctttttcgt ggaattgtct taaggcctga tcaataatat
     4981 ctaaatccgt ttctgtttca atcaatggcg agagtacatc gtattcggcc tttttagttt
     5041 gataatcctc ttcctttgga aaatttttct caatttcaac tttagcagta gtcaatttat
     5101 cctgtaattc atccaataag ttctgagttt tcacttggtc ctccttaatg tgatcaatcg
     5161 tatgctgaag tctttgaatt gtccccaaag gagaatacaa atctaaactg acagaatact
     5221 gattttctcc cacaatctta acagagaagg tttcaggaag aggttgattt gttggaagac
     5281 taagcatttt aatgtcaaat cctcgatagc ttgctagggt tcggaattct ttgctgtcag
     5341 cttgattatg acggataaga cggtgtaggg attcacctgc ttcagctcgt tgctcaaaag
     5401 cttgcttgcc tactgtcata gaaaatgcct ggtctttcga catttcagac tgttgaatgt
     5461 cgccttcata cttgcttaat cgtttctcaa gaatgggcat attttcttca cagtaagaga
     5521 ttgtatgacg atagtgatcc ttgctgcgtt gaaaggcacg tctttgattt tctaataggg
     5581 tcaaatcatt ctctagttcc atcttatatt taagataagg attacctgtt gctagtgcct
     5641 taaaatcaga agctgtcatg gtctgctcat caatatcttc tgcagcacga attggctcct
     5701 tagaagtcat aatctgctta atataacgga gtttgttctc ctgagttgcc catagataat
     5761 tatcaaacga acctttggta atgtagtggt aaatatccac ttccttattt tcatttcctt
     5821 gtcggataat ccgtccattg cgttgctgaa tgtcacttgg tctccagggg acatccaggt
     5881 gatgaactgc tttcatcttg ctctgaacat ttaaacctgt tcctcctttt tcagttgagg
     5941 caaggagaat ccgcacttct cctgcattga cctttcgaga caaactattc ttcttttcat
     6001 cactattggc atcatgtaca aaggcaattt ccttactagg gattcctcta tcaactaata
     6061 aagcctttat ttcagaatag acatcaaagc cattatcctt tttcttaggt gttccaatat
     6121 ctgaaaaaat catctgagta gccttatttt ccattccctc acgataaatt ctttcaacat
     6181 tatccactac ctgaagcagt ttatgattgt ctgctagact ataactagag tccaataaac
     6241 gcatatcaat agctagtttt cgtgcctcac ccgtaatttt taacatgtta tcctggctcg
     6301 gatcaactgt tccacatttg accatatctg acctcataac taattcttct aaatagagtt
     6361 tctggttttc agttaactca ctctcaatag ggataatatg agcttctgga acaggtaaat
     6421 ccaacatatc ttgtgtttga atgtcggctg tttctttata gattttcatc aactcaggta
     6481 gattgacaaa ctttttaaat cgtttcttag gttggtactt atcccctgta ggagctaatt
     6541 ccatagagtt ttgaatttct ccaaaagcac ctacccaaga gtcaaaataa tcaacttgat
     6601 agcgttttaa gatatctggt tgaatatagt tcatcatagt atacaactca ctaatagaat
     6661 tggaaacagg tgtccctgtc gcaaagacaa tatttttaaa atcatgttct tcctgaatct
     6721 gtcgaacctt catctccata tccacgttct tcttagaggt tgtattggta atacctgcta
     6781 cattcccaag tccagtaatt ggacgaatat ttttaaagtg atgtgcttca tccacaaaga
     6841 gaaaatcaat tcctaagttc tcaaaatcaa taaaactatc acgattgaaa cgttggagtt
     6901 cttccaattg tttctctaga ccacttattg attgttctgc ttctttaacg gtatacttat
     6961 tttcagaatg tgttttaatc tctcgtagtt cattgagttt atcctctata tagttcatct
     7021 gtctttcctt actgacaggg attttttcaa attgagaatc cccaatgaca atagcatcgt
     7081 aatctcctgt aatgatacgt gacacaaatt gttttcttct cgccttcaca aaatctttct
     7141 tagtggtcac aaagactttt ttagtaggga aaaatttcat gatttcttgg ccaaactgag
     7201 cagacaaact agagggcacc acatacaagg gcttatgaac catccccaac tcctttaatt
     7261 taaacccagc accaagcatg gtcaaggtct ttcctgaacc tacctcatga gctaataaag
     7321 ctcttttttc ttctacgatt ctttgaatag cattctcttg atgaggacga agactgatgt
     7381 tttgtgccaa gccatcaatg actagatggc taccatcata ctctcgacta actgttcgat
     7441 tataaagacg attatagctt tcctcaatga cctgttggac ttctggatac cgtgagacaa
     7501 attcttgaaa gagctcttgt aaatgctgct cttttgctct tagaacagag gttttttcca
     7561 aatctgtgat agtctttttc ttttcccctt ccgtaacagt catggtaatc gtcggttggt
     7621 tcgaattaag taaattctca aaaatctttc ttcctgtatc ataacgtgac ccactaactc
     7681 caagactact atctttggca cttggatagc gataaacaaa tgatgtcctt aaatgaacct
     7741 ggccatcgac aggattcact tcaatgactt gttcaacatc tggcgaagac aattcaaatt
     7801 cacgattggt aaaacattca aaggcaaatt taccataaac ggattgagga atccaacgtg
     7861 accctatttt gaactcaata tctgccagat gaatccgtgg agggcgaaca gattctaaca
     7921 aatctaaagc atgagaccag tcaaattctt ggttgttttc atctactaat agttgaacta
     7981 cttctatctt attgagaata tctcctgaca aaaactggtt cttagaaaga tattttcttt
     8041 cccctcttaa ataactttct ggatccatta aaacctggtc acctaactca tctaaaatag
     8101 cagcttggct atgttcggga taaattgata ccatatagtc taaatcaact cctctaccat
     8161 ccgataaact ggagtttaag gcatctaaag ccgttgatac tcttgtaatc tctctctctg
     8221 gtctaaccaa tgctttctca aaggctaaag attttttata ttttactttc tggtctttag
     8281 aatcgatgta ttcatcttct aaacttgcta gtaaagaata cttatcgtca ctatcaaata
     8341 agttccgatt gactgaggca ttcaagtatc caaattgact tacaaagcgg tcatagttat
     8401 gattgagttt actaagtaac acctgaaatt ctgtccgact ataatcttga tgacgttgaa
     8461 tttcaattaa ggattgatag gtctctctca aatcaaccat gcccttaatg cgactaatat
     8521 ccttatccga taagggactt tcataaaaga cagttttttt gaacagtccc ttatatttcc
     8581 ctcttttact cgcttcttct gacttgtaaa catctagtgc ttcctcttct gtcaaatgaa
     8641 gttgcacgaa tcggtctatt ttatgttcag acaaagaact gtcccaagct ttaaaatctc
     8701 ccttctcatc tacataataa ctaatttcgt ctacttttga acttttccga atgccatgcg
     8761 tatctcggta ataaatttga tttccctcat atccaaaaga atagagcgct aagtcctcac
     8821 gtatacgact tgggatagaa ttatccactt cttcttggat aaaaacaggt gctttcaaag
     8881 aattgtcaat ttgtttaggt gcttccacat tctcgaatgc tttcattatt tcagtagcta
     8941 atgtttctga ttccccctta acattgaggg ttcctccatt aaaattacgt acttcatatt
     9001 cacccaaaac ttgtgtattg tatttcccat caaaataagg attgatccag acacgcttat
     9061 cctcctcaaa gggaatagaa ccactaaaaa caagttcctc ttcattaaga ttctttgctt
     9121 gattcttttg aaagaagagg agatctgtgg tcactcgagt acctgcaatc tttttaaaag
     9181 ccgtatccgg caaacgaact ccccctaaaa aatgagtgtt ggttttaatc tcttgtaaga
     9241 cattatctgt ccgcttatcc attgtcccga tagatgagat aatcgacact tgtcctccgt
     9301 ctcttactaa atcaagtgag tgtttgacaa agtaatcatg aatcatataa ggtttatcat
     9361 agtttttatc ggcaatgcga aaatttccaa atggaacatt cgttaagact aaatcaaaac
     9421 tattattttg atagggaact tcttcaaatc ctcgcacttc aatgtggaca tttggatgga
     9481 gttgttttgc gatttcgcct gtcacactgt ctaattcaac cccatagagt tctgatttat
     9541 ctcgtatact tctaggcatc gccgcaaaga agttcccagt ccccatagaa ggatctaata
     9601 tccttcctcc ctcaaaacca tcatccagta atttttgcca aatctggcga ataatcattg
     9661 ggtctgtata ataggctgtg agagaacttt gtttcatggt cgagtattct gatttactta
     9721 ctaaactctt aagagttaaa cgttctgctt catactttgg attgagttca tcgaaaaatt
     9781 cattggcaag accaccccat ccaacatact tagctagtag ttcttgttct tctggattcg
     9841 cttgtcgtcc ctctttttct aatcttttaa caaggtcaat tgcagcgata ttcgtttcaa
     9901 ttttttctcg atttgtctta gggtaaaagt cctctaaatc atctggaaaa acgaaatctt
     9961 gaacagggat atccaactct tctatacttg aaataagagt ttctgtttcc ttatcctttt
    10021 cattttcttc ttccagatag gaaaacaaat ctatttcctg actttcactt gatgaatcaa
    10081 tctcaatctc tgaatcttct ttttcaagtt ctacaagaga caatacttgt tcaatctctt
    10141 ccaaactgtt caagtataag ataggatttt cttcaaataa ctggttggaa tcattgaata
    10201 gctctaggcg aactaagtca tttaactggg cattttcaat cgaaaccaac tgaaatactt
    10261 gacctttata acttacttgt gaaccgattg gatattccct caaagcttct tctacaattt
    10321 tatctacttt agatagggaa tgaacttcca cattttcttc tacctggtca atattatcta
    10381 aaggtaagac cgcttcctct acttgcttag atttttcgct tctagccttt acatcatcaa
    10441 aatgagtaat aatcttcagc ttttgtgtca gtggtagttg ttggtagttt ccttcatgat
    10501 ggacaagatc cgtaacggta tctaaagtag ccatcaattc tgaacccaaa taggctagaa
    10561 catctccctc tttttcttca aaatgaatct caagaacctc tttttctagt tctttggaat
    10621 caaccattag aagaaattcc tctatatctc tactaatgcg gtcagataga ttattcgcaa
    10681 cccgataaac attgactaaa ttgacctcct gattctgatg acatacaagc tcacttaatg
    10741 gtgttagctt ttctttttca gattggatgt aaaatcgagt ggaaaggtta taggttgcga
    10801 cttccagaac taagtttttc tcaaaattac ttaattgaga taatttacct agacccgctt
    10861 gcccaaaatt tcttgtgtaa ctttctaaat cttggtcatt attttcagaa ctggcctcta
    10921 ggaaagaaac aatatcttga tgaaacgaat agacatgagg tatctcttct atcttaccct
    10981 cttcagccaa aaaactctct accaagtcct gccatttctc atcaaaatgt tgcgcttgat
    11041 atcgaagaaa taggtccatt ttctccctat cttttggaat tgttccacga aacatagcca
    11101 ataattgcat tacttccata ctcgctcctt tcatctatac gaaaatagga gaatcacatg
    11161 attcccctac aactctatct ccatttcaac ctcttgagga ctggtttcta aatcactaga
    11221 caaaagagaa acggtatctg ttctcatgta gtaagtatat aagtcttcta ggtcttgttg
    11281 attttcaatc cctgtttcaa tactaatcag agcctgtata aattccttgt agtgactctc
    11341 catcatatct gaaatctttt gtttcatttc cataaatact gaggatggtt cagttaaact
    11401 tgtttggttt atgttttgta aatccttttc taagggattt gttccttctt ctacttccac
    11461 taaatctgaa cgtccatcac catcagaatc tgcgctaaga ggattggttc ctagcgccaa
    11521 ttcttgagca tcagttagtc catcttgatc cgaatcacgt tgataaatgg cttccatata
    11581 cttccttctt tcctagttaa tcttttttca ctccaatcct accaaatatt ccttgaaaga
    11641 agtatcacaa caaaaaagag aacgagccaa aactgcgttc tcctgaaaat gataaaacat
    11701 tactattaaa atagattaca tgtaaaactc taatcaggca gagcctgcta actcatgaca
    11761 gaaaaagatg tttagcctat agtgctggca ttaaaaaatc ctcacacatc acgcaaggaa
    11821 atcgctaaat aaaacaggct ttgaccatgt cgggattaat tgcagcacat tccaaggatc
    11881 cagctttttc ttttttttat aatcgaattt cccaaagagt aagcaagatg aaggccgtca
    11941 ttgcttatgc tcataagatt ctcagaatca tttacaaact actttctgcc aagcatactt
    12001 atcaaaaaga gaaggggcta ggactgagga aacagttcta acgccaaaac taaaaaattt
    12061 caattacacg ctagtacagg aattgatttt ttacgctttc ttactgtttt tttagaaaaa
    12121 aatagcataa aaatctagtt atccgcataa aaactggact tatcacactt tatcaaggtc
    12181 aaaaccactc aatttactac taatttacta cttatgaatg agctttgata cgacgattta
    12241 tccttgaaaa gtgaagatat aaagatactt ccaataaaat ttgaatattt aataggtaga
    12301 cacttcaaaa aatgaggtgt ctattttttt acccgatttt gaaaggaagt gaacttatga
    12361 aaacaaaaaa tcaagaatca aaaggtcgtt ccccactctt taagaccatc aaacattcat
    12421 tcagccaata aaaaagaaag gataggtaaa aatatggaac ttaaatttgt gattcccaac
    12481 atggaaaaaa cattcggcaa tttagaattt gctggcgagg ataaagtcgt tcagcgaaga
    12541 atcaacggac ggctaactgt cttatcaaga agctataatc tctattctga tgttcaaaga
    12601 gcagatgata ttgtggtggt gcttcctgct gaagctggcg aaaaacattt cggctttgag
    12661 gaacgtgtga agttagtcaa tccacgtatt accgcagagg gctacaaaat cggcactcgt
    12721 ggttttacaa attacctttt acatgctgac gacatgataa aagaataaag aaagagagga
    12781 aaaatgatga gattagcaaa tggcattgta ttagataaag acacgacttt tggagaattg
    12841 aaattctctg ctctacgtcg tgaagtgaga atccaaaatg aagacgggtc ggtttcagat
    12901 gaaatcaagg aacgtaccta tgacttaaaa tccaaaggac aaggacgcat gattcaagta
    12961 agtattcctg ccagcgtgcc tttgaaagag tttgattata acgcacgggt ggaacttatc
    13021 aatcccattg cggacaccgt tgctactgcc acctatcaag gagcagatgt tgactggtat
    13081 atcaaggcag acgatattgt gctgacaaag gattctagtt cattcaaagc tcaaccacaa
    13141 gcaaagaaag aaccgacaca agacaaatag tcgctaggta gaaaggagac tttttcgcat
    13201 gaaacagcgt ggtaaaagga ttcgcccatc tggtaaagat ttagtctttc attttacgat
    13261 agcgtcactc ctgcctgttt tcctgctggt tgtcggactg tttcatgtga agacaatcca
    13321 gcagatcaac tggcaggatt ttaacctatc acaagcagat aagattgaca ttccctattt
    13381 aattatcagt ttcagtgtcg caattcttat ctgcttgctg gtagcgtttg tattcaaacg
    13441 ggttcgctat gatacggtta aacaacttta ccaccgtcaa aaactggcaa agatgatact
    13501 tgaaaacaag tggtatgaat ctgaacaggt caaaacagag ggtttcttta aagatagtgc
    13561 tggtcgtaca aaggaaaaga taacctactt ccctaaaatg tattatcgac ttaaaaatgg
    13621 cttgatacag atacgggtgg aaatcacgct gggaaaatat caagaccaac tcttacactt
    13681 ggaaaagaaa ttagagagtg gcttgtactg tgagctgacg gataaagagt taaaggattc
    13741 ctatgtggaa tatactttgc tctatgacac catagccagt cgtatttcta ttgatgaagt
    13801 agaagctaaa gatggtaaac ttcgcttaat gaaaaacgta tggtgggaat atgataagct
    13861 ccctcatatg ttgattgctg gtggtacagg tggcggtaaa acttacttta tactgacact
    13921 gattgaagcc ttgcttcata cagattcaaa actgtatatt cttgacccga aaaatgctga
    13981 ccttgcggac ttaggttctg tgatggcaaa tgtctactat agaaaagaag acttgctttc
    14041 ttgcattgaa acattctatg aagaaatgat gaaacgtagt gaggaaatga agcagatgaa
    14101 gaactataag actggcaaaa attatgctta cttaggtctc ccggcacact tcttaatctt
    14161 tgatgaatac gtcgctttca tggaaatgct gggaacaaaa gaaaacaccg cagttatgaa
    14221 taagctgaaa cagattgtca tgttaggtcg tcaagctggc ttctttctaa tactggcttg
    14281 tcaacgtcca gacgcaaaat atttaggcga cggaatccgt gatcagttta atttcagagt
    14341 ggctttaggt cgtatgtctg aaatgggcta tggcatgatg tttggcagtg acgtacaaaa
    14401 ggatttcttc ttaaagcgaa tcaaaggtcg tggctatgtt gatgtaggaa caagtgtcat
    14461 atcagagttt tatactcccc ttgtaccaaa aggatatgat ttcttggagg aaattaaaaa
    14521 gttatccaac agcagacagt ccacgcaggc gacgtgcgaa gcggaagtcg caggtgtgga
    14581 ctgatcttgc tggctggtgt ggcaatagcc acgccagcac ttaacccccc gtatctaaca
    14641 ggggggtaca aatcgacagg aaacagtcaa aaaaacatta gaaaatcctt tggttacaag
    14701 ggatttacaa aatttcagcg tatgtcaaat gggctttaaa agttgacata cgcctttttg
    14761 attggaggga tttttactga atgaacaaac ttggttacag catttaaaag aaaaacgctt
    14821 ggcttatgga ctatctcaaa accgtttagc tgttgcgact ggtattacaa ggcagtatct
    14881 aagcgatatt gaaacaggaa aagtcaagcc atcagaggat ttacagcagt ccctttggga
    14941 agctctggaa cgcttcaatc ccgacgctcc ccttgaaatg ctgtttgatt atgtaaggat
    15001 tcgctttccg acaacagacg tacagcaggt ggtcgaaaac atcttacaac tgaaactgtc
    15061 ctattttctt catgaggact atggtttcta ttcttattca gagcattatg ctttaggcga
    15121 catattcgtc ctttgctccc atgaactgga caaaggagtt ctggtggaat tgaaaggtcg
    15181 tgggtgcaga caatttgaaa gctatcttct ggcacaacaa agaagctggt atgagttctt
    15241 tatggacgtt ttggtggctg gcggtgtgat gaaacgcctt gaccttgcca ttaacgataa
    15301 gacagggatt ttgaatatcc ctgtactcac tgaaaagtgc caacaggaag aatgtatctc
    15361 cgtcttccgc agttttaaaa gctatcgcag tggcgaactg gtacgcaaag aggaaaagga
    15421 atgtatggga aacaccctct atatcggttc attacaaagt gaagtttatt tctgtatcta
    15481 tgaaaaggac tacgagcagt acaagaaaaa tgatattccc attgaagacg cagaagtaaa
    15541 aaaccgtttt gagattcgat tgaaaaatga gcgtgcctat tatgcagtcc gtgatttact
    15601 cgtctatgac aatccagagc ataccgcctt taaaattatc aatcggtata tccgttttgt
    15661 agataaagac gattccaaac ctcgttctga ttggaaactg aatgaagaat gggcttggtt
    15721 tattgggaac aatcgtgaac gattaaaact aaccacaaaa ccagagcctt actccttcca
    15781 aaggacgctg aactggctat ctcatcaagt tgccccgacc ttaaaggttg cgattaaact
    15841 tgatgaaatc aaccagacgc aggttgtaaa agacattctc gaccatgcga aactgacaga
    15901 ccgacacaag cagattttga agcaacagtc agtaaaagaa caggacgtga taacaacaaa
    15961 aaaataactc aaatacaaat tcattgaata tagagaggag aacattttta tgaattttgg
    16021 acaaaacctt tataactggt ttctatcaaa cgctcaatca ctggtgcttt tagcaatcgt
    16081 tgtgattggc ttgtatcttg gcttcaagcg tgagtttagc aaactgattg gctttttaat
    16141 tattgcgatt attgcggttg gcttagtctt caacgctgct ggagtaaaag acattttact
    16201 agagctattc aatcgcatta ttggtgctta aataaaaccg ttcttttgtg gaatataagt
    16261 ggttttctta tgttccgcaa aggaatggta caccaaacga agtgcggtag ggatttttga
    16321 atctctacaa agaaaggacg tgaatatatg gacgatatgc aagtctatat tgcgaattta
    16381 ggcaaataca atgagggcga attggtcggt gcgtggttta cctttcccat tgactttgag
    16441 gaagtcaaag agaaaatcgg cttgaatgat gaatatgagg aatacgccat tcatgactac
    16501 gagttaccct ttacggttga cgaatacact tccattggcg aactcaatcg actatgggaa
    16561 atggtatcgg aattacccga agaattacaa tcggagctat ctgctctgct cactcatttt
    16621 tcaagcattg aagaactaag cgaacatcaa gaggatatta tcattcattc cgattgtgat
    16681 gatatgtatg acgtggcacg ctactacatt gaagaaacgg gtgctttagg cgaagtacca
    16741 gctagtcttc aaaactatat tgattatcaa gcctatggtc gggatttaga cctttcagga
    16801 acgtttatct caaccaatca tgggattttt gaaatcgtct attaaatctg tcggtacatt
    16861 actactggca gattttctat tttacggggt ggctcaatca gctaccccta ttttttatga
    16921 aaggattgat tacatgaaga aaatacgaag ctgtaccagt atctggtctg tggaaaaggt
    16981 actgtattct atcaatgatt ttagacttcc gtttcccata acctttacgc aaatgacatg
    17041 gtttgtcgtg tcactctttg cagtgatgat acttggcaac ttgccccctc tttccatgat
    17101 agagggagca tttctcaaat actttgggat tcctgtggct ttcacatggt ttatgtctac
    17161 aaaaactttt gatggtaaaa agccttatgg atttttgaag tctgtcattg cttatgcact
    17221 gcgaccaaag ctgacctatg caggaaaaaa agtaacgctt ggcagaaacc agccacaaga
    17281 agccattaca gcagttagga gtgaatttta tggcatatcc aattaaatac attgaaaaca
    17341 atctcgtctg gaataaagac ggggaatgtt atgcttacta tgagcttgtt ccttacaatt
    17401 actcatttct aagtccagaa cagaaaatac aagtgcatga ttctttcaga cagcttatcg
    17461 cacaaaatcg tgatggcaaa attcatgctt tacaaatcag tacagaatcc agcatacgtt
    17521 ctgcacaaga gcgttccaaa aatgaagtca ctggcaagct caaagcggtt gcctatgaca
    17581 aaatcgacca acagacagac gctttaatat ccatgattgg cgaaaatcaa gtgaactacc
    17641 gtttctttat cggctttaag ttgcttctca acgatcagga gttttctatg aaaagtctta
    17701 ccgttgaagc aaaaaatgct ttgtctgatt ttgtctatga tgtgaaccat aagctgatgg
    17761 gcgattttgt tagtatgagt aatgatgaaa tcctgcgttt tcagaagatg gaaaagctct
    17821 tagaaaataa aatctctcgt cgtttcaaaa tccgcaggtt agataaggac gacttcggct
    17881 atctgattga acacctttac ggacagacag gcactgccta tgaagagtat gagtaccatc
    17941 tatcaaagaa aaagctggat aatgaaacgc tgattaaata ctatgacttg attaagccta
    18001 ctcgctgttt ggtggaagaa aaacagcgat atttgaaaat ccagcaggaa gatgaaaccg
    18061 tctatgtagc ttactttacc attaacagca ttgtcggaga actggacttc ccgtcctctg
    18121 aaatcttcta ctaccagcaa cagcaattta cattcccgat tgatacgtca atgaatgtgg
    18181 aaattgtagc gaatcgtaaa gccctatcta ctgtccgcaa taaaaagaaa gaactgaaag
    18241 acttggataa ccacgcttgg caaagtgata atgaaaccag ctccaatgtg gcggaagctc
    18301 tggaaagtgt gaatgagctg gaaaccaatt tagaccaaag caaggaatct atgtacaagc
    18361 tgtcttatgt ggtaagggta tcagcaaatg atcttgacga actcaaacgt cgttgtaatg
    18421 aagtgaaaga tttttatgac gatttaagcg taaaactggt acgaccattt ggggatatgc
    18481 tcggcttaca tgaagaattt ttacctgcca gcaagcgtta tatgaatgat tatattcaat
    18541 acgtgacctc tgatttcctc gctggtttag gttttggtgc tactcaaatg ctgggggaaa
    18601 atgaggggat ttatgttggc tacagcttag atactggacg caatgtctat ctgaaacctg
    18661 ctcttgccag tcaaggggtt aagggttcag taaccaatgc gttagcgtcg gcttttgttg
    18721 gttcgctggg tggtggtaaa tcctttgcga ataaccttat cgtctattat gcggtgcttt
    18781 atggggcaca agcagtgatt gtagacccaa aagcagaacg tggcagatgg aaagaaacct
    18841 tgccagagat ttcccatgaa atcaatatcg tcactctgac ttctgatgag aaaaacaaag
    18901 gcttacttga cccttatgtg attatgaaaa atcccaaaga ttctgaatca ctggctattg
    18961 atattctgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
    19021 gaaaagccat tcgtgcagta accaatagtg aagtacgagg gttgatgaaa gtgattgagg
    19081 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
    19141 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
    19201 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
    19261 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
    19321 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
    19381 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
    19441 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
    19501 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
    19561 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
    19621 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
    19681 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
    19741 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
    19801 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
    19861 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
    19921 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
    19981 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
    20041 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
    20101 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
    20161 tggtaagaat atgcagacct tagctggtgt gagtgcaaac ggattttcaa cagagggttt
    20221 ctatgttgga ttcctcttac tcttgatttt ggttcttggg gtttatgttg cctatacggg
    20281 actgataaag agagaaacca caaaggcaat tcatgccatt atgaattttg tgctggtgtt
    20341 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
    20401 ttcatcagac atcagtaatg ccagtttatc acttggcacg aagattgtca tgccccattc
    20461 cgatagtcaa ggcaaggaca gcgtggactt aatcagagat agcctgtttt ccatacaggt
    20521 tcagcaaccg tggctactgc ttcaatacaa cagttcagac attgaaagta tcggtattga
    20581 ccgtgtggaa agcctgctct ccaccagccc agattccaac aatggcgaag acagagaaaa
    20641 aattgttgcg gaagaaattg aagacagaag caataccaat ctaaccatta caaagaccat
    20701 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
    20761 atttctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
    20821 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
    20881 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
    20941 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
    21001 tgcttttcta cagattgtga cctttgcagg aatctacttc aagctgggcg atttaatgag
    21061 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtgtga tgagaaaacc
    21121 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gttccatgac
    21181 tactctaggg gctgggtctg ccattgttac aggtaaaaaa ggacagtcgg gttcggggag
    21241 ttctgcaagg acacaagcag atcactcccg accagacgga aaggaaaaat caacacttgg
    21301 aaaacgtatc ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagacac
    21361 tgctagtggt ttgaaagaac aggttaaaga tttgccgacc aatgcaagat atgcagtata
    21421 tcaaggaaaa tccaaagtaa aagagaatgt ccgtgattta accagtagta tttctcaaac
    21481 caaagcggac agagccagtg gacgcaagga acagcaggaa caaaggcgaa aaaccattgc
    21541 gaagcgtcgc tctgaaatgg aacaggtcaa acagaaaaaa cagcctgctt cttctgttca
    21601 tgaaagaccg actacaagac aagaacaata tcatgatgaa cagacctcaa aacagtctaa
    21661 tattcagact tcatataagg aatctcaaca agccaaacaa gagcgtccag cagttaagtc
    21721 cgatttttca agtccaaaag tggaacgcca aggcaatacc gttcaagaaa aaaccgttca
    21781 aaagccagca acttcaacca ctacagcaga tagaacttca caacgtccaa tcacaaaaga
    21841 acgtccgtct actgttcaaa gagtaccact acaaaataca agaagtagac caccaatcaa
    21901 aaccgccacc attaagaaag tcggtaagaa accatgaagt tgaaaacttt agtgattggt
    21961 ggttctggat tattcttgat ggtcttctca ctgcttctgt ttgttgccat tttattttca
    22021 gatgaacagg acagcggaat ttccaatatt cattatggag gtgtgaatgt ttccgcagaa
    22081 gtgctggctc ataagcctat ggtagaaaaa tatgccaaag aatatggcgt tgaagaatat
    22141 gtcaacatac ttcttgcgat tatacaggtg gaatcgggcg gtactgcgga agatgttatg
    22201 cagtcctcgg aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag
    22261 caaggtgtga agtatttcag tgaattatta gccagtagcg aaaggctcag tgtagattta
    22321 gaatcggtta tccagtccta caattatggt ggtggtttct tagggtatgt ggctaatcgt
    22381 ggaaataaat atacctttga actggctcaa agtttctcaa aagagtattc aggtggcgaa
    22441 aaagtgtctt accccaatcc catagccata cctatcaatg ggggctggcg atacaactat
    22501 ggcaatatgt tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat
    22561 gatacggtac aagccatcat ggacgaagca ctgaaatatg agggctggcg atacgtttac
    22621 ggtggagctt ccccgactac ttcttttgat tgtagcggac tgacacaatg gacgtatgga
    22681 aaagctggaa ttaacttacc acgaaccgca caacagcaat atgatgtgac ccagcatatc
    22741 ccactatcgg aagcacaagc tggcgatttg gttttctttc attctaccta taacgctggc
    22801 tcttatatta ctcatgttgg gatatacctt ggcaataacc gtatgtttca tgcaggcgac
    22861 ccaatcggtt atgccgactt aacaagcccc tactggcaac agcatttagt gggagcagga
    22921 cgaatcaaac aatgagaaag gaagatttaa tgatgaaatt tagaaaaaat cagaataaag
    22981 aaaaacagat accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cataaaaagg
    23041 ttgtgattgc cttgtgggta cttttagggc ttagtttcag ctttgcgata ttcaagcact
    23101 ttacagctat agatactcat actattcacg aaacaactat catagaaaag gaatacgttg
    23161 atactcatca tgtagaaaat tttgtagaga actttgcgaa agtctactat tcatgggagc
    23221 aatccgataa gtccattgat aatcgaatgg aaagtctaaa aggctatctg acagatgaac
    23281 ttcaagctct caatgttgat acagtacgca aagatattcc tgtatcgtct tctgtaagag
    23341 gatttcagat atggacggta gagccaactg gcgacaatga gtttaatgta acctacagtg
    23401 tagaccagct cattacagag ggagaaaata caaagaccgt ccactctgct tatatagtga
    23461 gtgtctatgt agatggttct ggaaatatgg tactggttaa gaatccgacc attaccaaca
    23521 tacctaagaa atcaagttat aaaccaaaag ccattgaaag tgaggggacg gttgattcca
    23581 ttacaaccaa tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga
    23641 cagccagtga actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt
    23701 acatctttca agaactggta aatcctattc acaatcgtaa ggataatcaa gtcacggtat
    23761 cgctgacagt ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt
    23821 tggtacttga aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt
    23881 attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat tccttgacaa
    23941 atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc
    24001 aaggtattct taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt
    24061 ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag
    24121 tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat
    24181 aaaagtattt atcactggga tttttatgcc cttttgggtt tttgaatgga ggaaaatcac
    24241 atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca
    24301 gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca
    24361 acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata
    24421 acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat
    24481 ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct
    24541 gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg
    24601 attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt
    24661 tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg
    24721 tatcctaata tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag
    24781 ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc
    24841 gaacaagagg aaagcataag atttcataat tgttccctgt tccctgttta tcacggaagt
    24901 gcaaaaaaca atatagggat tgataacctt atagaagtga ttacgaataa attttattca
    24961 tcaacacatc gaggtcagtc tgaactttgc ggaaaagttt tcaaaattga gtattcggaa
    25021 aaaagacagc gtcttgcata tatacgtctt tatagtggcg tactgcattt gcgagattcg
    25081 gttagaatat cggaaaagga aaaaataaaa attacagaaa tgtatacttc aataaatggt
    25141 gaattatgta aaatcgataa ggcttattcc ggggaaattg ttattttgca gaatgagttt
    25201 ttgaagttaa atagtgttct tggagataca aagctattgc cacagagaga gagaattgaa
    25261 aatcccctcc ctctgctgca aacgactgtt gaaccgagca aacctcaaca aagggaaatg
    25321 ttacttgatg cacttttaga aatctccgac agtgacccgc ttctgcgata ttatgtggat
    25381 tctgcgacac atgaaatcat actttctttc ttagggaaag tacaaatgga agtgacttgt
    25441 gctctgctgc aagaaaagta tcatgtggag atagaaataa aagagcctac agtcatttat
    25501 atggaaagac cgttaaaaaa agcagagtat accattcaca tcgaagttcc accgaatcct
    25561 ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat
    25621 gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg
    25681 atacgctatg gctgtgaaca aggattgtat ggttggaatg tgacggactg taaaatctgt
    25741 tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct
    25801 cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt
    25861 agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaccga tgctcctaaa
    25921 tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa
    25981 atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt
    26041 agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag
    26101 ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag
    26161 tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa
    26221 taaagatttg aattaaagtg taaaggagga gatagttatt ataaactaca agtggatatt
    26281 gtgtcctgta tgtggaaata aaacacgatt aaagataagg gaagatactg aattaaaaaa
    26341 attccccctc tattgtccga aatgcagaca agaaaattta attgaaataa agcagttcaa
    26401 agtaactgtg attacagagc cagacgcaaa gacgcagagc cgataaaatg agattaatac
    26461 aatctcattt tatcggctct ttccgttatg tatggattct tttaattagt cttcgatgtt
    26521 tcttgcttcg ttgataccgc tggctaaaga ttccattaag gatagttctt tgtctgtaaa
    26581 gctatccatg tatttctcta tctgtaatcg tcgggtgctt tttaccaagt tattagcagg
    26641 taagaaaaat tcatcaacgg aaacatgaag taacgataca aggtcataaa gaacttgtat
    26701 gctggggtgt tgccctttat tttcaatatt agttaagtac cgtgggtcaa tttcaatcaa
    26761 tgctcccact tgttcacgag ttaaacctcg tttcaatcga gcttctttaa tggctaaacc
    26821 aaaggctcta aaatcatatt tatcttcttt tttacgcata gtagaccacc tctatacatt
    26881 ttattgttcc tactgaatta aaaacaggta tagaaaaacg tgttatatgg tttataggtt
    26941 tatatttaat aaaaagcact actaaacgcc aataaaaaaa accgttatat ggtagtgcta
    27001 tttacgctgt taaaatattg tatattactt ccaaatggcg gtttgttgga ggtcaacgtc
    27061 gccatgaagt acatcatata caataaattt ccttacattg ggttcttgtc aaaaaaagtc
    27121 gtctatctgc aatagataag tacgtccacc aatgtggttt tataaatcat atagatagaa
    27181 taacagaagc atgtaaacag agaaataaat ctgtttatat gcttttttgg ctattcagaa
    27241 cttttttaca aagtttattt atcagtaatg caacaaatcc ccctttcaca ttgggactaa
    27301 gagtgaaagg agataaacga gcaaggctca cttcctttcc tagacagaaa gggggtgaga
    27361 aacatgaaac catcttcttt tcagaccaca atagaaaatc agtttgacta tatctgtaaa
    27421 cgtgctatgg aagacgagcg aaagaattat atgctttatc tttcaaggat tgcaaagcgt
    27481 gaggtgtcct tttcggatgt tggcgattat cttgttagcc agtttgcgac aacagataac
    27541 tattcaactg actttcagat ttttacactc aatgggttat cagtaggcgt tgaaaatgat
    27601 ttgttgagtg aagcattacg tgagttgcca gacaagaaac gtgaaattct actgctgttt
    27661 tactttatgg acatgagcga ttcagaaatt gcagacctgt tgaaattgaa ccgttctact
    27721 gtctatcagc atagaaccag tggactagcc ttaattaaaa agtttatgga ggaatttgaa
    27781 gaatgaaaac acaatatcct atgattccct ttcctctcat tgtaaaggca acagatggcg
    27841 ataccgaagc gattaaccag attctacatc attacagagg gtacataacg aagcgttccc
    27901 tacgacttat gaaagatgaa tatggcaatc aaagtatggt cgttgatgaa gtcttacgtg
    27961 gaagaatgga aaccagactg attacaaaga ttttgtcatt tgaaattaag taatatcctc
    28021 tctcctttcg tggaagcgtg ctaaaccatt ccacgcttcc cgaacaggga ggtttgttat
    28081 tccaccaaag catattgagc tttcaatgtg ttttgatagg ctaacgagcc attgttcttt
    28141 gaaaactgaa taaaagtaat cgaatacgtt tcgataagaa aagagccaac ggaactaacc
    28201 gccatgacct atcttataaa gatagcgagc gattcatgtt agtgatccga gaagcaatct
    28261 ttagcaggat tgcctgcaac gacattctta tcgtgataat gatactccca tacagtcaat
    28321 agtccgagcg tgataaaacc gtcgcaggca atgagtatgg ctacatgaga accatgcagg
    28381 ggtggaactc ccgtgagctt tgctaaagct gttcgattgc tggtaaaaca acttttatga
    28441 aatccaaata agtgatttgg aaaggaggat tttatgaagc agactgacat tcctatttgg
    28501 gaacgttata ccctaaccat tgaagaagcg tcaaaatatt ttcgtattgg cgaaaacaag
    28561 ctacgacgct tggcagagga aaataaaaat gcaaattggc tgattatgaa tggcaatcgt
    28621 attcagatta aacgaaaaca atttgaaaaa attatagata cattggacgc aatctagcgt
    28681 cgccaaaggg tcttgtatat gataaaatag tattaagtcg tatcaaggct ctttccataa
    28741 aggaaaggag caaatgccat gtcagaaaaa agacgtgaca ataaaggtcg aatcttaaag
    28801 actggagaga gccaacgaaa agacggaaga tacttataca aatatataga ttcatttgga
    28861 gaaccgcaat ttgtttactc gtggaaactt gtggctacag accgagtacc agcaggaaag
    28921 cgtgattgta tctcacttag agagaaaatc gcagagttac agaaagacat tcatgatggt
    28981 attgatgttg taggaaagaa aatgacactc tgccagcttt acgcaaaaca gaacgctcaa
    29041 agaccaaagg ttagaaaaaa cactgaaact ggacgcaaat atcttatgga tattttgaag
    29101 aaagacaagt taggtgtaag aagtattgac agtattaagc catcagacgc taaagaatgg
    29161 gctattagaa tgagtgaaaa tggttatgct tatcaaacca tcaataacta caaacgttct
    29221 ttaaaggctt cattctatat tgctatacaa gatgattgtg ttcggaagaa tccatttgac
    29281 tttcaactga aagcagttct tgatgatgat actgtcccta agaccgtact aacagaagaa
    29341 caggaagaaa aactgttagc ctttgcaaaa gctgataaaa cctacagcaa aaattatgat
    29401 gaaattctga tactcttaaa aacaggtctt cgtatttcag agtttggtgg tttgacactt
    29461 ccagatttag attttgagaa tcgtcttgtc aatatagacc atcagctatt gagagatact
    29521 gaaattgggt actacattga aacaccaaag accaaaagtg gcgaacgtca agttcctatg
    29581 gttgaagaag cctatcaagc atttaagcga gtgttagcga atcgaaagaa tgataagcgt
    29641 gttgagattg atggatatag tgatttcctc tttcttaata gaaagaacta tccaaaagtg
    29701 gcaagtgatt acaacggcat gatgaaaggt cttgttaaga aatacaataa gtataacgag
    29761 gataaattgc cacacatcac tccacatagt ttgcgacata cattctgtac caactatgca
    29821 aatgcaggaa tgaatccaaa ggcattacag tacattatgg gacatgctaa tatagccatg
    29881 acgctgaact attacgcaca tgcaacattc gattctgcaa tggcagaaat gaaacgcttg
    29941 aataaagaga agcaacagga gcgtcttgtt gcttagtagt acaaatgaat ttactactta
    30001 tttaccactt ctgacagcta agacatgagg aaatatgcaa agaaacgtga agtatcttcc
    30061 tacagtaaaa atactcgaaa gcacatagaa taaggcttta cgagcattta agaaaatata
    30121 aaaagataat tagaaattta tactttgttt caaataaaaa agatgactta ctgaacctgt
    30181 aagccaccta atcagtcggt ttattctggt gtctgccacc gcttggcccg tacgtccaag
    30241 attgctatcg gattttgttc tggtgtccgc caccgcttgg cccttatgtc caagattact
    30301 atcagattga ccatgagccg accactcatt tatagacata ttgtatcttt aaatagctac
    30361 aatttcaagt gtaaactctt ctatttggtc gtataagcaa aactgatggc actatctgct
    30421 tgagagattt ttctaaaggt atagttaggg ttgcccccat agttggtttc agacacaagg
    30481 aaagaaccat catcatagac cttctctaca aaagccacat gaccatagct ggctggtgta
    30541 ccatgtgtac ctcctacaaa agaaacaata gcacctgctc ttggtgtaga gcccgtttcc
    30601 ccaccaagac ttgaagctgt cgcaacccag tcttgaccat ttcccatggt attaatgatt
    30661 gaaatctttt ctccatttct accttttaat tttaagccta actggttcat acgagccgca
    30721 acaccccatg tacattgtcc ataagcatag gccataccat ctccaccacc aggaacagaa
    30781 tgatcataca agtccccacg aaccccttca agggattgtg gatcactttt tgcctgtcct
    30841 ccatttgttt gactaaagcc tttttcaatt tggtaatacc attccgttgc tctggtttgt
    30901 ctttccagta gtttgtcacc agaatttccc tcccaatagg tcagaaagag ttgggccaga
    30961 ttggctgcac tgcctgtatt tttaaagaaa tcctttaacc aactttgata gtaaggacta
    31021 tccccatgaa gcataaaatc aagttgtagg tctaaatcat accatttctt attttgggtg
    31081 cgtgcataat ttaacaaggc tgtatgccgt gttgacccat ctgcggtatc tgtccattgc
    31141 cctaaaccta gacctctatg aagaatatta ggataagcac cactataaat ggctggacct
    31201 ccaatcgcta accagctttc atcatcccat gaggaatcgg tagcgccaac aggaggagat
    31261 aaataatctc cttcagctcg tttaggatta atagaagact ctaccgacca atttcctaaa
    31321 attgccgcaa tggcttgggg acttgcccct tgagatttca aaaactcata aatatgtttt
    31381 gctcgttcaa actcatcccc accaaactga ccaatggctg gtaagatagt ggtctgaagt
    31441 tgaatgactt ttggaaaata aaattgcgga ttgacataca ccaatttttc tttcttgttt
    31501 ttatactttt gataggaaac tttcaaacct gtatcgtctg gtgtttcacc aataatatca
    31561 cccgttagga ctcttgtccc ctcaatcgca cggccattat gaatagaata caaggtcaat
    31621 cgactctcat tctctccttt tccgttagtg agaataacat catctccgtc tagagataca
    31681 acaccatcca ttggtgcgac aattgtttgg tgagccttcg cttctagtag aatatactcc
    31741 tgaagagtag gttttccgtc taaatcatag tatccataac gataagtcat ggttagacta
    31801 tcttcgttgc ttttcccctc aaatggattg tccaattcct gcatggaagc atagacaccc
    31861 tcttctttta gttcctttat ttcctcttga tcgtctctcg atagtttata cttaggagtt
    31921 tcatagaggt cttgcatgga tttcaaatca tccccatcgt ttaaatcatg ccacaaagta
    31981 gacagataat ccttgtaaga ttctgaacta aataagtgaa ctggtttgtg taactcatag
    32041 tcatggaatt taaagttcat ataccccatc acatcatcaa cttttgtgta ataagtaatt
    32101 cctttgtcat ttgtgcgagt atgttctgca tcttcccaag ttaggtgggt ataagctttt
    32161 gttaattcaa attcatcttg ttgaatcaaa ctagcagatg aaaatcctaa aaagaagctc
    32221 atcataagta aaagaagaaa gactattcct ccaactatcc aggttacagg atttccagcc
    32281 acaaatgtaa agaaggaaaa ggctgctttt aatttttgat agatatttcg gacacttaaa
    32341 agaccttgtt tctttaattt tcgataccga tttttaaagg aacttggatt atctttagtt
    32401 agtttccatc cttttccatc cttaaaatga tggtatcgct cttttgtgtt ggtcagtctt
    32461 ttcttggtaa aacgacctgt tgcttgtcct gttttgacac tagcttttcc aagattataa
    32521 gaaagccgac tgtagcgttt cccttttcta atggtctcat gaagtgtgcg ataactttct
    32581 aaatcttcat tttctgaagc taactctcca ccttcacgtc caaggacata aagaaaggtt
    32641 ttggctttcc tactgatttt tttgaactta taggcttgct tagtagattt tagattctct
    32701 tttgcggctt tgacttcttt cttagctttt aattcttcta gattcttccc ttgaaggaaa
    32761 aaattggatc ttactttcgt ttcctgaccg tagaaaaatt tttgattggt ttttctttct
    32821 ttacgactct cttttctttc ttcttttgct tcaaccttag cttccttaaa ttgtttcttg
    32881 gctattttca atcgtttcct agcatgaggc aatcgtctat ctcttaattc tttccgattc
    32941 aaaagtgatg gaggactatt ttgaagaata tgattgtagt cttcatttgc ttgttttact
    33001 ctagcctttg aagcttctct catattctcc agcttttgct ttatctcatt tttccatgct
    33061 ttttcatcca gtacagcgga atcttttttc tgttttctca cctccttctt tccttgtttt
    33121 aagaatttct tctcatcttt tagacttctt ctaaatgcct ttcgggcacg tatgatttct
    33181 ctcttatcct tcatttacct tccccttaat tagaagccat tttatcagga tctgtactca
    33241 tgatatcaaa caattgtgta ccttggggaa tcttattttt aaagggaacg acaactgaac
    33301 cagcttttat tagtcctgcc cctttttctg gattgactag gtatttttcg agttcttttg
    33361 acaagcctaa gagttgaact agttcttctc ggtcattttt tgcttgcttg aggagaatca
    33421 taaattcact atttgcaata atccgtctac catttggatc taacaataag gtttcgacat
    33481 tttgggttat cccagtcgga ctggctccat attttctgac acgactccac aatttaaaga
    33541 agaaatcact ggcatattta tctaataaga gaagctgcat ttcatcaaaa taaatccagg
    33601 tcttcttccc taatttttgg ttccgaacaa cacgattcca tatctgatca aaaaccacca
    33661 taagggcgat ttgtttcagc tcatctccta actttttaac attatagatt aagaaattag
    33721 atcctgtctg aatattggtc ttatgagaaa aaatatcaag agaaccttcg acatacagtt
    33781 ccatatcaag tgctaaattc tgcgcttctt cttctggttg ttgactcaag acaaagaccc
    33841 attcttccaa agaaggctct ttaaatgact gataggtaag tctggtaact cggtcaataa
    33901 tcgatttttc tctcccatcc atttttctat ccaataactt gccgataaag gataaaagaa
    33961 attctgattt tacctttaca ggatcttcat ccatattttc ttcagacaag tcaaggacat
    34021 tgagataggt ttgggaatca ggcgcaatat caatcatttc tcccccaaaa gcccggccaa
    34081 tgacactata ttctgcctct ggatccacga tgataatttc ggtattttca ccagattcct
    34141 tgattttggt cgtgataatc tcatgcttgg ttgccatccc tttcccagct ccagatgttc
    34201 ctaaaatcaa accagacggt gtatttaata ggctgcgatc aatggtaata atattgcttg
    34261 atatttgatt gataccataa tatttcccac tgcggtcttg taagtctact gaagtccatg
    34321 gagagttcac tgctatattg gacgttaata aactccgtga tacgccctct aaaaaatcac
    34381 aaccaaatgg cagcaaacta ttaaaagctg cttcttgcat atatggaagt ttatcaatca
    34441 ttaggtcatt tgagccggcc acttgttgga tagtgtctag ggcttgtttg agttcttctt
    34501 catcctgacc aaaaacaccg atcaagaaga ctgtttgaaa tagtttatct cctgtctctg
    34561 tcatggtttt taagagttcc tcagcttcat cgatattact ttctaataca tgacctactt
    34621 tttccaaata gatacctgta cgagctagtt tttgttgttc cccaatcttt tgggattcca
    34681 tcaaggtttt ctttgttcgt agtttcttca tggcatccgc cttagtcgaa ctttgggcat
    34741 ggaggctcac aatcaattcc aaatctcctt gcatgaggtc tcggataaac tgatccccta
    34801 attccatacc gtagtctctc acatagacaa tctgcaataa gcggtcattg atttgtaggt
    34861 aattcttgtt tttaaagtcc aagagattag gtgctatgaa gtgacgagtt gtctgaccag
    34921 atttcgttaa atcacggtaa gaaaaaggaa gatggtgttc tcctctaagc atatcggcca
    34981 acaagttcac ccgttcttct ccagccaaag attcaaatcg tgcatcaatt tctgagaaac
    35041 cactcttgaa atattcgcct atttgagaca aggaacgata ggcttgtttg ggattagaat
    35101 cctttctacc aaagctaatc agtttcacag ccgaaaagtt attttctcca ctgtctaaat
    35161 tttgattcat catccgattc aattctttac gatagctatc atacccatct tctttttcct
    35221 catacaaaac actttgtctg aatttttcta aattcaatct tttattaaag atagtcaatt
    35281 ggaagttggt tttgtcgtct aaagagttaa tcaaatcaga atacttctca atgattgccc
    35341 ccttatcttc taaaccaacg gtctggtaat tgacatcacc aagtaaatag ctttgtgaaa
    35401 aataatcttc ttttacctgc atcagaccgt tttgatacaa ggcttgataa gaaagagtat
    35461 tagccgttga gggtaacact tcctcttttt taactttaac ttcttccttt ttattagtca
    35521 ttgaagtttt ttgtttcttt aatgtatttg attttctttt catgttcagg tcctttcttt
    35581 cctgtaattg tgcgtagggg aaccgttaat tcaaaatgaa gacggtattt caaataatgt
    35641 tcaaaatata aatcattggg tttatagact ccaaatagca tgaggggaat agtaaaggca
    35701 aacacaaaac cgtaaacaaa ccaatctcca aattgccaga aaaagagatt caagcccaaa
    35761 acaataattg tgacaataaa ggctggtaaa acaaagatga tttgtcttgt ggtgaagcct
    35821 agccaagccc tgtgttggta ttttgagatg tctttaaaga cacgtgtatt catgactttc
    35881 ctttctaaaa aggctaagaa gcaatcactt cctagccttt atctaattac atacctaaga
    35941 ttgagcgagc cgtacgttga gaaccaacga gggcaataat cagtaagata gcttgtacca
    36001 aactaccaaa cataatcgca agtgattgca agactcctgc accatttgaa acagctattt
    36061 tcccagcaga ttcaaataaa ggaacaagag aaacaatcag aaaaatgaga accccttgta
    36121 ccgcatagac cataatattt tttaaatagc caataccaat ggacttccat tcatcgctta
    36181 aaaatgttgg aatcgtaaga ggggcaaatg gaatcatgag gtagagttga ataaatcgaa
    36241 tggataccaa aagattgacc atggctgcac ttactatccg aacaagccaa atgaggaggg
    36301 cgaaaaagcc cacaatcata cggccaataa atcctgaacc ttttaatcca gagattgtat
    36361 catactttgc cccaccgtga gccacaatcg aagcaacttg ttcaatagcg tgactcgcaa
    36421 tcccgatgat agcttctaca ataacggtag tgttggtaat tacaactgcg accataatat
    36481 aactaatcaa catcggagct aatgcctcaa aggtcatcgc tccaccagag ttagcaattt
    36541 tctttgccat cttcgaaaat tctaagatga gaacaactga taaaatcgca actccaagag
    36601 gttgcatgac acttttagta atactagaca tataagtcca aacggttgga ttgtagctag
    36661 atagagattt aatcagatct accgtagatt gtaaatctac attaaatcct tcaaataaat
    36721 tttcagctga tattttttca gatgcaaggt aaacaaaggg tgagactaaa ctaagattca
    36781 tgtcattgtt tatcctccta aattgaaatt tgagttacaa aggctccagc agccccgacc
    36841 ataactccac cgacaatttc cagaatagca ttccgaacac ctggtccacc atctttaatg
    36901 ttggttgcaa gattgacaat ccccacaaca acgagaaagg caccaacggc aatcaatccc
    36961 ttctgtaaca aagacatagc ttgtgcaaac atggcactag cgtctactcc atagacaaaa
    37021 cctttaaaat gcgtaatcat ctatttcctc ttttctattt ttattttaaa ctagattcaa
    37081 aagttaaatc acgaattcta aggccttcaa gatgattttc ttgtctttga tttaaaggat
    37141 tgatttgata gttccaccac cgttcatcgg tttcttgatt ggctaggtac ttccagtttg
    37201 gatgcttagt agaattgtat tttttgcttt taaagacagg catattggca attcgaacca
    37261 ggcattcatg ccgtttcata tttccgactt catcaggtgt cattagatca cgagcaatct
    37321 tttgatgaga aagggatcct gaacctgtct ggccaaagga acgactagta tttcgaacat
    37381 caatggtttg tttaccgagt aaaccactca taaatttaaa ggtatcttca tcattcccac
    37441 ctaagtagac taagctatca cagttcccaa gaatggtttt ccaagcttct ttttctttat
    37501 agagcccttg aagttgggca atattttgta gaataggaac gagactcatg ttccgagaac
    37561 ggactgttga ggtttgttca gcaaaatctg gaatttctcc gatatttggg aactcatcta
    37621 agtaaaatct cacatgaaga ggcaattgcc ccttaaaatc aatatctgct tgtcttgtca
    37681 gggtttgaaa tacggttgaa aaaaagaggg ctgaaagaaa gcgaaaggta ctatcgttat
    37741 ctgggataac taagtaaacc attgattttt ccttgcccca tgtcttcata tcaagggtat
    37801 ctcttttggt caaatccatg acactttgaa tattgaagag ggcaaattta gcagtggtta
    37861 cagctataac agaatccagg gtcttatcct tataattttg aaaatctgcc caatttcgca
    37921 tggtaaaatt ttcagtccca tactttttag cataattttc aaataaaatt tctaagacac
    37981 ttttttcttg gttttcaccc ttggataagt gtttaatgag ttttgagatt tcagcaaaac
    38041 ttggataacg ccctcgtttt tttcgctctt ccacttcttt tttttgacgt ttcaacaagt
    38101 tttggtattc tttttgactt aaacgacttt cttctatgag ctgttctctt gttttgggtg
    38161 gattatagaa atcgaccaag taggaggcta aagctcgtac caaagtcata gaagcttcat
    38221 cccaaaatgg atcactacgg gatccagagc ctttggtatt attaaaataa accgtcagca
    38281 tgcgattcaa atcattttct gtctctatat agcgaaaagg attgaaacca tctgagttct
    38341 tcatattgac taaatctaac acctttactt ggtaaccatt ttctaaaaag agtttacctg
    38401 ttttctcggc caaatgatct ttaggatcca ctacaatatt agaactattc atctgaatca
    38461 gattgggttt cacaaagcga aatgtcttcc cacttcctga acctccgatc accacaatat
    38521 tcttatttct atcatattgg ggtggttttt tatctaacaa tgtcaaacga acatcttgtg
    38581 ctaagatcgt atcatgagaa aattccttac cgtaaaagag cttcttttct tttagagttc
    38641 caaaacgggc gctcccgtat tctacccctt ctcggtattg ttttttacca gtctctagat
    38701 agagataaac cagtaacatc atcacaaaac ctagtagaaa aaaaacactt gattttccag
    38761 taaaagaaac attccatggc gactgaagaa cttgatcttg accttccatc agaagatgag
    38821 tccatttatc taaagtattt ccagtatagg aatcatacaa aagcgtcaaa cgatgaaaaa
    38881 gatagcctag taagatacct aacagtgaga atagtaggaa tttctttcca ctgtacatca
    38941 tctcaccatc tctttctgtt tgacggcccc ttcttgtcta aaggttattt gggatttagc
    39001 ttcatcaatt gcatcgtcta aagacttatc catggtaaaa tcagctaatt tctctggatt
    39061 attaaccatt ttttctaaca gatggtctaa atgattgtct agaattgaac ggtctttcgt
    39121 gtagaaatgc agagaatccc cttgccaagc gatggctaaa ggaatctctt ctttttctaa
    39181 aaaagcttta aatttctcta tatcaattgg tttgtctaaa aaatcttttt tcagattaat
    39241 cgtatcaatc gaataaggag attgtagcaa ttcttctaat ttctgcaccc ctatcttata
    39301 ggccgaatcc tgtgctaaag cctgacgtct agaccattct agaatcttta aaagactttt
    39361 tacagtaaat aaaagactac gctctgcata ttgaactgcc attcgttcct gttgttcaga
    39421 ggacatctga tgcctccttc ttaacaaata gcagctttcc ttctttataa cgataagcta
    39481 tcaatttctg acgttgttta atcgacttga caacctgcag tagatctctc gaataaggtt
    39541 ctcgaagagt aacttctact gctttcccat caacgatacc aacacgttta aattcaatgt
    39601 agtctttttt gagatgtcct aagcccatac caaagggaaa gtgatgaatc aattggatgg
    39661 tataaccacc tttatttccc atttgtttca aatcatacaa gtttactacc ttcatgatta
    39721 actcctttat ctagtttgtc gcatcgttta agtctggaaa cgataaactc cgtgtctgtt
    39781 agaaaattct cacacacgtc ttgtgccagt tgcccttcac agggaagtac tctcagtccc
    39841 tacttacaca ggcacgctaa tcaagacgga gtggattcaa ttttcaaaga acaggtagct
    39901 ttattataga taagagtagt tgaaattttt atcacatttt ttatttccct gtagagctaa
    39961 attcttacta aacaaagatt ttgaaataaa agactaacat tgttacataa cgttagcatt
    40021 tcatttccct aaaacaagct aatttggtct aaaaaagcag caaactataa actagtaggt
    40081 tccacaccaa atgtagcccc atactgcccc ataagtccga tttgtagcgt acaagcccta
    40141 aaaacatccc cagcgataca tacaaacacc aagctagaat ggttcctgga tgatgtgcta
    40201 aggcaaataa aacacttgtc aaagcaactc gaatatctaa ttttctaacc aagttccata
    40261 taatttctcg atacagaaat tcttcaacca tactcgcatt gattaagaac aataaaaatg
    40321 aaaaccaagg aacttgatgt tgaagcccaa ttaaatttgc ttgattcgta gttccttgag
    40381 catgaatcag actaaaacat agacttataa tcagtaggct gacaaatcca ataccaagcc
    40441 attttatcct agttttcata ttaaccttga tcacttgttt tcgttgacca tacatccata
    40501 aaaaagaaat gagagaccca taatagagaa tctgtagtat agttacctca ccgatacaaa
    40561 acaatttcag taagtatagg gatagtaata
//
LOCUS       DAVBDG010000013        40178 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBDG010000013 DAVBDG010000000
VERSION     DAVBDG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.013"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(63..1094)
                     /locus_tag="VQ526_000612"
     CDS             complement(63..1094)
                     /locus_tag="VQ526_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2554368.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLSHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIKTGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(1349..2032)
                     /locus_tag="VQ526_000613"
     CDS             complement(1349..2032)
                     /locus_tag="VQ526_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2554369.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGSDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(2044..2988)
                     /locus_tag="VQ526_000614"
     CDS             complement(2044..2988)
                     /locus_tag="VQ526_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2554370.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            3120..5951
                     /gene="uvrA"
                     /locus_tag="VQ526_000615"
     CDS             3120..5951
                     /gene="uvrA"
                     /locus_tag="VQ526_000615"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2554371.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDINQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            5944..7005
                     /locus_tag="VQ526_000616"
     CDS             5944..7005
                     /locus_tag="VQ526_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2554372.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            7137..7535
                     /gene="spx"
                     /locus_tag="VQ526_000617"
     CDS             7137..7535
                     /gene="spx"
                     /locus_tag="VQ526_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2554373.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            7601..8170
                     /locus_tag="VQ526_000618"
     CDS             7601..8170
                     /locus_tag="VQ526_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2554374.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            8257..8523
                     /locus_tag="VQ526_000619"
     CDS             8257..8523
                     /locus_tag="VQ526_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2554375.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            8527..8946
                     /gene="ruvX"
                     /locus_tag="VQ526_000620"
     CDS             8527..8946
                     /gene="ruvX"
                     /locus_tag="VQ526_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2554376.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            8962..9267
                     /locus_tag="VQ526_000621"
     CDS             8962..9267
                     /locus_tag="VQ526_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2554377.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            9573..10823
                     /locus_tag="VQ526_000622"
     CDS             9573..10823
                     /locus_tag="VQ526_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2554378.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            10820..11362
                     /locus_tag="VQ526_000623"
     CDS             10820..11362
                     /locus_tag="VQ526_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2554379.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            11508..13040
                     /gene="cls"
                     /locus_tag="VQ526_000624"
     CDS             11508..13040
                     /gene="cls"
                     /locus_tag="VQ526_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2554380.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            13298..14836
                     /locus_tag="VQ526_000625"
     CDS             13298..14836
                     /locus_tag="VQ526_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2554381.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVGLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            14956..17163
                     /gene="nrdD"
                     /locus_tag="VQ526_000626"
     CDS             14956..17163
                     /gene="nrdD"
                     /locus_tag="VQ526_000626"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2554382.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHHLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            17175..17315
                     /locus_tag="VQ526_000627"
     CDS             17175..17315
                     /locus_tag="VQ526_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554383.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            17359..17865
                     /locus_tag="VQ526_000628"
     CDS             17359..17865
                     /locus_tag="VQ526_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2554384.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            17858..18448
                     /gene="nrdG"
                     /locus_tag="VQ526_000629"
     CDS             17858..18448
                     /gene="nrdG"
                     /locus_tag="VQ526_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2554385.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLGQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELSDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            18445..19062
                     /locus_tag="VQ526_000630"
     CDS             18445..19062
                     /locus_tag="VQ526_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2554386.1"
                     /translation="MKKKGLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELSQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAYELESLQRDIFALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            19317..19625
                     /gene="rpsJ"
                     /locus_tag="VQ526_000631"
     CDS             19317..19625
                     /gene="rpsJ"
                     /locus_tag="VQ526_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2554387.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            19842..20468
                     /gene="rplC"
                     /locus_tag="VQ526_000632"
     CDS             19842..20468
                     /gene="rplC"
                     /locus_tag="VQ526_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2554388.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            20493..21116
                     /gene="rplD"
                     /locus_tag="VQ526_000633"
     CDS             20493..21116
                     /gene="rplD"
                     /locus_tag="VQ526_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2554389.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            21116..21412
                     /locus_tag="VQ526_000634"
     CDS             21116..21412
                     /locus_tag="VQ526_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2554390.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            21430..22263
                     /gene="rplB"
                     /locus_tag="VQ526_000635"
     CDS             21430..22263
                     /gene="rplB"
                     /locus_tag="VQ526_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2554391.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            22367..22648
                     /gene="rpsS"
                     /locus_tag="VQ526_000636"
     CDS             22367..22648
                     /gene="rpsS"
                     /locus_tag="VQ526_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2554392.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            22660..23004
                     /gene="rplV"
                     /locus_tag="VQ526_000637"
     CDS             22660..23004
                     /gene="rplV"
                     /locus_tag="VQ526_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2554393.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            23017..23670
                     /gene="rpsC"
                     /locus_tag="VQ526_000638"
     CDS             23017..23670
                     /gene="rpsC"
                     /locus_tag="VQ526_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2554394.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            23674..24087
                     /gene="rplP"
                     /locus_tag="VQ526_000639"
     CDS             23674..24087
                     /gene="rplP"
                     /locus_tag="VQ526_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2554395.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            24097..24303
                     /locus_tag="VQ526_000640"
     CDS             24097..24303
                     /locus_tag="VQ526_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2554396.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            24328..24588
                     /gene="rpsQ"
                     /locus_tag="VQ526_000641"
     CDS             24328..24588
                     /gene="rpsQ"
                     /locus_tag="VQ526_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2554397.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            24614..24982
                     /gene="rplN"
                     /locus_tag="VQ526_000642"
     CDS             24614..24982
                     /gene="rplN"
                     /locus_tag="VQ526_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2554398.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            25060..25365
                     /gene="rplX"
                     /locus_tag="VQ526_000643"
     CDS             25060..25365
                     /gene="rplX"
                     /locus_tag="VQ526_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2554399.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            25389..25931
                     /gene="rplE"
                     /locus_tag="VQ526_000644"
     CDS             25389..25931
                     /gene="rplE"
                     /locus_tag="VQ526_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2554400.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            25949..26218
                     /gene="rpsN"
                     /locus_tag="VQ526_000645"
     CDS             25949..26218
                     /gene="rpsN"
                     /locus_tag="VQ526_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2554401.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            26503..26901
                     /gene="rpsH"
                     /locus_tag="VQ526_000646"
     CDS             26503..26901
                     /gene="rpsH"
                     /locus_tag="VQ526_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2554402.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            27093..27629
                     /gene="rplF"
                     /locus_tag="VQ526_000647"
     CDS             27093..27629
                     /gene="rplF"
                     /locus_tag="VQ526_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2554403.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            27713..28069
                     /gene="rplR"
                     /locus_tag="VQ526_000648"
     CDS             27713..28069
                     /gene="rplR"
                     /locus_tag="VQ526_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2554404.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            28087..28581
                     /gene="rpsE"
                     /locus_tag="VQ526_000649"
     CDS             28087..28581
                     /gene="rpsE"
                     /locus_tag="VQ526_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2554405.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            28553..28777
                     /gene="rpmD"
                     /locus_tag="VQ526_000650"
     CDS             28553..28777
                     /gene="rpmD"
                     /locus_tag="VQ526_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2554406.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            28922..29362
                     /gene="rplO"
                     /locus_tag="VQ526_000651"
     CDS             28922..29362
                     /gene="rplO"
                     /locus_tag="VQ526_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2554407.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            29375..30685
                     /gene="secY"
                     /locus_tag="VQ526_000652"
     CDS             29375..30685
                     /gene="secY"
                     /locus_tag="VQ526_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2554408.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            30836..31474
                     /locus_tag="VQ526_000653"
     CDS             30836..31474
                     /locus_tag="VQ526_000653"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2554409.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            31591..31809
                     /gene="infA"
                     /locus_tag="VQ526_000654"
     CDS             31591..31809
                     /gene="infA"
                     /locus_tag="VQ526_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2554410.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            31834..31950
                     /gene="rpmJ"
                     /locus_tag="VQ526_000655"
     CDS             31834..31950
                     /gene="rpmJ"
                     /locus_tag="VQ526_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2554411.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            31968..32333
                     /gene="rpsM"
                     /locus_tag="VQ526_000656"
     CDS             31968..32333
                     /gene="rpsM"
                     /locus_tag="VQ526_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2554412.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            32351..32734
                     /gene="rpsK"
                     /locus_tag="VQ526_000657"
     CDS             32351..32734
                     /gene="rpsK"
                     /locus_tag="VQ526_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2554413.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            32777..33712
                     /locus_tag="VQ526_000658"
     CDS             32777..33712
                     /locus_tag="VQ526_000658"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2554414.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            33724..34110
                     /gene="rplQ"
                     /locus_tag="VQ526_000659"
     CDS             33724..34110
                     /gene="rplQ"
                     /locus_tag="VQ526_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2554415.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            34375..34641
                     /locus_tag="VQ526_000660"
     CDS             34375..34641
                     /locus_tag="VQ526_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2554416.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            34651..35988
                     /locus_tag="VQ526_000661"
     CDS             34651..35988
                     /locus_tag="VQ526_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2554417.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            36232..36924
                     /locus_tag="VQ526_000662"
     CDS             36232..36924
                     /locus_tag="VQ526_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2554418.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(37027..38672)
                     /locus_tag="VQ526_000663"
                     /pseudo
     CDS             complement(37027..38672)
                     /locus_tag="VQ526_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(38662..39285)
                     /locus_tag="VQ526_000664"
     CDS             complement(38662..39285)
                     /locus_tag="VQ526_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554419.1"
                     /translation="MDEPLSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAI
                     SDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDG
                     YALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASK
                     IQVSEESTFEEELQKGNRIRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            complement(39271..39393)
                     /locus_tag="VQ526_000665"
                     /pseudo
     CDS             complement(39271..39393)
                     /locus_tag="VQ526_000665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(39370..39740)
                     /locus_tag="VQ526_000666"
                     /pseudo
     CDS             complement(39370..39740)
                     /locus_tag="VQ526_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289359.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(39753..40121)
                     /locus_tag="VQ526_000667"
     CDS             complement(39753..40121)
                     /locus_tag="VQ526_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2554420.1"
                     /translation="MTVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKSK
                     NMENAKKFIDFIISQEVQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIK
                     NKSDIVKKYNEVFTDIQSKQ"
BASE COUNT        12264 a         7518 c         9032 g        11364 t
ORIGIN      
        1 taatatgtga tgaaaatgcc agtaatgata ccgagaaaaa agctgagaaa cttttcccag
       61 ctttatttgt tatagtcaaa gagaatgact tgttcctgtg catctacatg agcatggacc
      121 ccaaagggta caattgctct tggagttgcg tggccgacat tcagattata gacaatcggg
      181 atattgctgt caatgatatc caatagtgcc tctttatagt cgtcatggaa agtttcatcc
      241 ataggttttc cgaccaagag tccattgatg accgcgaata tgccagtgtc ctttaaagtt
      301 agcaacattt ttttgaagtc ttctggctta ggcttttctt cgcttgtttc gagcaagagg
      361 attttccctt cccagtctga caagtcaggg aaaagtttgt atttttggca gagttccgtg
      421 ctatctgcgt atcgagagtt gtcaaagata tcgtagaggg attcgaggca accaccgagg
      481 attttcccct cgaactgagc acttccttgc aacaagtcaa aacctgtatt tgtatgactg
      541 acacgaggtg ttcccagggc cgtgggacta aaatcagttc gttcctcata ccaaacgtca
      601 ctagggcgga tttctgaaat tcttcccgtc ttaatcaatt ctttaaagta gtgaaggcta
      661 taggctagca tttctttgtc taattcacaa atgtctgcta aaaaggattg accataaaaa
      721 gtcttgattc ctaatttatg caacatgagg tggttcatgg ttgtatccga gaagccaaga
      781 aaaatttttt gcttgataac cttttggagt tggtcatttt caaaaagata aggtagcaag
      841 cgataggtat cgtctccacc gatggcacat aggatcatgt cgatgctatc atcagaaaag
      901 gcatgaatca aatcctctgc acgagcttca ggatggtcct tgataaagtc taatcctttt
      961 aacgaatggg acaaaaagat gggattgagt cccagatcct tgagacgttg gatacccaag
     1021 tccacttcgt gtttgacaaa atcctctccg ataatgccac tagataaact aacaatacca
     1081 atagtagaaa ccatatctca tcctcctaga aatagattga gcctatttta tcacaaaata
     1141 taagagaaag ctatgtggga tttcagaaga atacttttaa tactcttcga aaatctcttc
     1201 aaaccacgtc agttttatct gcaacctcaa agctgtgctt tgagcaacct gcagctagct
     1261 tcctagtttg ctctttgatt ttcattgaat ataactcaaa aaagtaagtg aaaaagcaac
     1321 cgcacctgca gttgctttta tcgttttctt attcttgata gcgtgttggt cctgcacttg
     1381 cactacggat attcaagcgt ttcttgagat agaagcgaag ggctaggagg gctgctccaa
     1441 taatagctag tggcaatgga tccagtactg ggttaaggct agctggtagg aagcttgttg
     1501 caaagaagac aagcaaccaa aggaacatag aagctaggat aactagtaca gatttccaga
     1561 aaggtggacg ttgactgcga tccatatctg atccatagta ttggtaaaca aagtagtaca
     1621 tcaagtagaa ggcaaatcca ccaaccagtc caactaatag aagagtaatc aatccatagc
     1681 cgaaagcttg gtctgccgca aagaaggttg tgagggcgct gacaagggca aagaggctag
     1741 tgatgaaaag agctgagtcc ataatcatca gttttgggtc atcattttct tttggatgct
     1801 ctttttcgta ctgctctttg acagtgaagc tatgagccca atgagttggt gcgccgtata
     1861 gggaacgggc agttgtacct ttagattgct cctcaaggat ttggggaatg acttcctcaa
     1921 aaatagtctg gatttcagcg tctgttttcc catctttgat gaattgttgg gtagcaatgt
     1981 ggacaaactc ttggtttttc ttagttaatt tttgtagatc aatttgagac ataggaactc
     2041 ctcttagaac cattttttat ggatgagata gagagtgagc gagacactca tagcaaaggc
     2101 gataaagacg attaaccaga aggcatttgg ctctccgttt agggggattt cattatcctt
     2161 aaagttcatc ccgtaggcag aaaagaccat ggttgggatg gacatgacga tggtcacaag
     2221 ggccaaggtt ttcatgatgt tgttctggtt gttagaaatg atagaggcaa aggtctctgt
     2281 catagaatgc aagacgtttc cataaatatc tgccatctcg atggcctgtt gggtttcaat
     2341 cagggtgtct tcaagcaggt cttcgtcctc aaggtatttc ttgatattgc tggttgaact
     2401 ggtcaatttc ttaatcacgc gctcatttgt tttgagggag gccttgaaat agacgatagt
     2461 tttttccaat tccatgagct caatcaattc ttcattacga gttgattgat gcagttgact
     2521 ttcgatttgt tcactcttgc ggtcgattga acgaagggct gttaggtaaa gctctgcatt
     2581 gcgataaaga atttgaaaga taaaacgtga acgcatgaag gtatagaaat tacgcaatcg
     2641 acggttgata aagacatcaa ggacaggtag tggttccaaa cacgtagtga taatggtttc
     2701 ctcagtgatg ataataccaa gcgggatggt tacgtagtag gtgcggttat ttctttcctc
     2761 cgtgaccggc acgtctacga taatcagggt atactcgtct tcaatggtaa tacgagacat
     2821 ttcttccgca tcgagcggtg ctcgaaggtc ggcaatatca atatcgaagg tgttggcgat
     2881 ttcgagtgat tcattttgag tcggattgac gagattgatc caagtacccg gttcaagcgt
     2941 atcgatctct ttaaattcag ttgttgtaga gagaaaaact tgtttcatat cccttatcct
     3001 ttctcatttt tcagattttt tcacactgta ctattatact acaaaatcgg ccttttgggt
     3061 aatagaatct cacttttttt ggtataatgg taagcaataa tggactagaa agaacaaaga
     3121 tgcaagataa aattgtcatt catggggcgc gtgcccataa tttaaaaaat attgatgtgg
     3181 agattccgcg agacaagttg gttgtcgtga ctggcttgtc aggttcaggg aagtccagtc
     3241 tggcttttga tactctctat gcggagggac aacgtcgcta tgtagagagt ttgtccgcct
     3301 acgctcgtca gttcttggga aatatggaaa aaccagatgt agatgccatt gatggtctca
     3361 gcccagctat ttccatcgac cagaaaacga ctagtaaaaa tcctcgctcg acggtgggaa
     3421 cgacgactga aatcaatgac tatctgcgtc tcctctacgc acgtgtgggg acgccttact
     3481 gtatcaacgg acatggagct atcaatgctt cttctgtgga gcaaatcgtt gataaggttt
     3541 tggagttacc tgaacgccag cgcttgcaga tcttggctcc tgtcatccgc aagaaaaaag
     3601 gccaacataa gagtgttatc gagaaggttc agaaagacgg gtatgttcgt gtccgtgtgg
     3661 atggggaagt ctatgatgtg accgaagtgc cagagttgtc taagagcaag caacacaata
     3721 tcgatgtcgt ggttgaccgt attgttatca aggagggcat tcgtagtcgt ctctttgatt
     3781 ccattgaggc tgcccttcgt attgcagaag gttatgtcat tatcgacacc atggacgact
     3841 cggagttgct cttctctgag cattatgcct gtccagtttg tggatttact gtcccagagt
     3901 tagaacctcg tctcttctct ttcaatgctc cctttggctc ttgtagtgag tgtgacggct
     3961 tggggattaa gctggaggtg gatactgatt tggtagtgcc agatgccagc aaaaccttac
     4021 atgagggagc gctggcacct tggaatccta tctcatccaa ctactatcca aacatgttag
     4081 agcaggccat gaaagtcttt ggagtggcta tggataagcc ttttgaggac ctgtcagaag
     4141 aagataagaa cttgattctc tacggctcag atggtaagga attccatttc cattatgaaa
     4201 atgaatttgg tggtgtgcgc gatatcgaca ttccgtttga gggagttatc aataatatca
     4261 aacgtcgtta ccatgaaacc aatagcgatt acactcgtac tcagatgcgt ctctacatga
     4321 atgagctgac ctgcggaacc tgtcaaggct atcgtctcaa tgaccaggcc ttgtctgtcc
     4381 gtgtgggcgg ccagcaaggg ccacatattg gagaaatctc agacctgtct atcgctgacc
     4441 acttggactt ggtgagccag ttaactttgt ctgaaaatga agccatcatt gctcgtccca
     4501 ttctcaagga aatcaaggat cgtttgacct tccttaataa tgtgggtctt aactatctaa
     4561 ccctgtcacg ttcagcagga accctttcag gtggggaaag tcagcgcatt cgtttggcaa
     4621 cccagattgg ttccaaccta tcaggtgtcc tctatatcct agacgagccg tcaatcggtc
     4681 ttcaccagag ggacaatgac cgcctgattg ccagtctgaa aaagatgcgt gacttgggca
     4741 atactcttat cgtggtggaa cacgacgaag ataccatgcg tgaagctgat tatctgattg
     4801 acgttggtcc tggtgccggt gtttttggtg gtgagattgt tgcagcaggt acacctaagc
     4861 aagtggctcg caacagcaag tctatcacag gtcagtactt gtcaggcaaa cgtgtcatcc
     4921 cagtaccaga agagcgccgt gtcggaaatg gtcgttttat tgaagtgaca ggagcgcgtg
     4981 agaacaactt gcaaaatgtc acggctcgct ttccactagg aaaatttatc gcagtgacag
     5041 gtgtgtcggg ttcagggaaa tcgactctaa tcaacagcat tctcaaaaaa gccattgccc
     5101 agaagctcaa ccgtaattca gacaaacctg gtaagtttaa gactattaca gggattgagc
     5161 atgtcgaccg tttgattgat attaaccaga gccctatcgg acggacgccg aggtctaacc
     5221 cagctaccta tacaggagtt tttgacgata tacgtgacct ttttgctcag acaaatgaag
     5281 ccaagattcg aggctacaag aaggggcgct tcagtttcaa cgtcaaggga ggtcgctgtg
     5341 aagcttgctc aggtgacggg attatcaaga ttgagatgca cttcttgcca gatgtttatg
     5401 tggcttgtga agtttgccac ggaacccgct ataacagtga aaccctagaa gttcactaca
     5461 aggaaaagaa tatttcgcag gtcttggata tgacggtcaa cgatgcggta gaatttttcc
     5521 aacacattcc gaaaattcaa cgcaaactcc agaccatcaa ggatgtaggt ctaggctatg
     5581 tgaccttggg acagccagct accacccttt ctgggggaga agcccaacgt atgaagttag
     5641 ctagcgaact ccacaaacgc tcgacaggaa aatctttcta cattctggat gagccgacga
     5701 cagggcttca tacagaggac attgctcgcc tgctcaaggt tttagctcgc tttgtagacg
     5761 ccggcaatac agtcctcgtc atcgagcaca atctggatgt tatcaagacg gcagaccata
     5821 tcattgactt gggacctgag ggcggtgtcg gtggtggaac catcatcgta acaggaactc
     5881 cagaagaagt agctgccaac gaagccagct atacaggaca ctatttgaaa ggaaagttac
     5941 atcatgaata aacgcgtaca agcatttcta gctaaaatgc aagaaaaaga actagatggt
     6001 atcatcatca acaatcttaa aaacgtctat tatttgactg gtttttgggg ctcaaacgga
     6061 acagtcttta tcagccgtga ccgtcaggtc ttagtgacag actctcgcta tatcatcgca
     6121 gctaagcaag aaaccagtgg ttttgagatt gtggctgatc gtgatgaatt ggctgtcatt
     6181 gcaggaattg ttaaggacat gaacttgact cgtatcggtt ttgaagatga gatttcagtg
     6241 tcttattacc accgtatgca ggcagctttt gcaggtttga acttgcttcc acaaactcag
     6301 tttgtggaag gtcttcgtat gattaaggat gaggcagaga ttgcagcgat tcgcaaggct
     6361 tgttctatct cagaccaagc tttccgcgat gcgcttgact ttatcaaacc aggaaaaact
     6421 gaaattgaga ttgccaactt ccttgatttc cgcatgcgtg agttgggagc atctggctta
     6481 tcttttgata cgatcctagc tagcggtatc aattcttcta aaccccatgc ccatccaatg
     6541 cacaaaccag tggagttggg agaagccatc accatggact tcggctgtct ctatgaccac
     6601 tatgtcagtg atatgacccg gactatctat ctagggcatg ttagcgatga gcaggcagag
     6661 atttacaata cggttctaaa agctaaccaa gccttgattg accaagctaa ggcaggctta
     6721 ggtttccgtg actttgacaa aatccctcgt gatattatca ttgaggcagg ttatggtgac
     6781 tactttactc acggcattgg ccacggtatt ggtctggata tccatgagga accctacttt
     6841 agtcagactt ctacagaaac tattaagaca ggtatggcct tgaccgatga accaggtatc
     6901 tatatcgaag gcaaatatgg cgttcgtatc gaggatgata tcctgattac agagacaggt
     6961 tgtgaattat tgaccctagc tccaaaagag ttgatagtca tttagttgtt tgtcaagaaa
     7021 aaagtacgaa aatgacgaaa aaagtcaaaa aaaattaaaa ataggtcgca agtcggatgt
     7081 tttttatggt ataatagact aaactgatag taacatgtag cgaaaggggt aggtacatga
     7141 ttaaaattta tacagtctca agttgtacta gctgtaaaaa agcaaaaacc tggctcaatg
     7201 cccaccagtt aagttataaa gaacaaaacc ttggtaaaga aggaattacg agagaagaat
     7261 tactggatat tctaaccaaa acagataacg gaatagccag catcgtttcg tctaaaaatc
     7321 gctatgccaa agcccttgga gtggatattg aagatttgag tgtcaatgaa gttctcaatc
     7381 tgattatgga aacaccgaga attttaaaga gcccaatcct tgtagatgaa aaacgcctgc
     7441 aagttggcta caaggaagac gatattcgtg ccttcctacc acgctctgtc cgtaatgtag
     7501 aaaatgcaga agcacgtttg cgtgcagctc tataaacatc aaggctggga gcacttccca
     7561 gtcttattct attttaattt caaaaagaaa gaagaaagaa atgaaaaaaa tagttcttgt
     7621 tagtctagct ttcctttttg tcctggttgg ttgcggacag aaaaaagaaa ctggaccagc
     7681 tacaaaaaca gaaaaagata cgcttcagtc ggcattgcca gttattgaaa atgctgagaa
     7741 gaatacagtt gtaactaaga ctttggtctt gcccaagtca gatgatggta gccagcagac
     7801 acaaacaatt acttacaaag acaagacttt tttgagtcta gctatccaac aaaaacgtcc
     7861 agtctctgat gagttgaaga cttatattga ccaacatgga gtggaggaaa ctcaaaaagc
     7921 tcttcttgaa gcggaggaga aggataagtc tatcattgaa gctcgtaaat tggcaggttt
     7981 caaacttgaa acaaaactat tgagcgcaac ggaacttcaa acaacgacta gttttgattt
     8041 tcaagttctg gatgtcaaga aggcttccca gttggaacat ctgaagaata ttggtttgga
     8101 aaatcttttg aaaaatgaac caagcaaata tatttcagat agattggcaa atggcgcgac
     8161 agaacaatag aaaatccaaa taaatagact gcctgaattg tagaaggtaa ggaattatgc
     8221 tataatggta ctattctaag gaaagagggt gttagaatgg gatttactga agaaacagta
     8281 cgttttaaat tggacgattc caataaaaaa gaaattagcg aaactttgac agatgtttat
     8341 gcttcgttga acgataaggg ttacaaccca attaaccaaa tcgtaggtta cgtattgagt
     8401 ggagaccctg cctacgttcc tcgttataat aatgcacgaa atcaaatccg taagtatgag
     8461 cgtgatgaaa tcgttgagga attggttcgc tactacctca aaggacaagg agtcgatcta
     8521 taacctatga gaattatggg attggacgtc ggttcaaaaa cggtaggggt ggcgattagc
     8581 gatccgcttg gttttacagc tcaagggctt gaaatcatcc agataaatga agaacaaggc
     8641 caatttggtt ttgaccgcgt taaggaattg gttgatactt acaaggtgga acgatttgta
     8701 gtgggcttgc ctaaaaacat gaacaataca agtggaccgc gcgtagaagc tagtcaagcc
     8761 tacggagcaa agctagaaga gttttttggt ttaccagtag actatcagga tgaacgcttg
     8821 acaacagtgg ctgctgagcg catgttgatt gaacaagcag atatcagtcg caataagcgc
     8881 aagaaagtca ttgataagtt agcagctcag ctgattttac aaaattattt agatagaaaa
     8941 ttttaatata aaggagaggc tatgtcacac gatcataacc acgaccacga agaacgtgaa
     9001 ctaatcacac tagtagatga gcaaggaaat gaaaccttgt ttgaaatcct tttgacaatt
     9061 gatggaaaag aagaatttgg taaaaactat gttcttctag taccagttaa cgcagaagaa
     9121 gacgaagacg gacaagttga aatccaagct tactcattca ttgaaaatga agatggaaca
     9181 gaaggcgaat tgcaaccaat cccagaagac tcagaagacg aatggaacat gattgaagaa
     9241 gtcttcaaca gctttatgga ggagtaaaaa cgtccggggg acgtttttag ccctgcgcta
     9301 gaaattagaa acgcaggtag tctgggagac tgtatcagcc caactccaga aatggggaag
     9361 agttggaacg tccagtgaac atttttaccc caactcttag aaattgagaa gagttggaac
     9421 atctagtgga tgtttttagc ccatattcac attcatagta gctagttatt agctaaccag
     9481 gtaagaggtc gggacgaaaa tcctggcctt gtttttatta gtaaccatac tttaatgcga
     9541 taattgattg gacttttgtc aaggagatgt gtatgtttga agtagaagaa tggctccata
     9601 gtcggattgg tttgaatttt cgatcaggtt tgggccgaat gcagcaagcg gtggatttgt
     9661 taggaaatcc tgagcagtct taccctatta tccacgtaac agggactaat gggaaaggat
     9721 ctaccattgc ttttatgagg gaattattta tggggcatgg caaaaaagtt gcgaccttta
     9781 cctcccctca tatcgtatcc atcaatgacc gaatctgcat taatgggcaa cctatagcag
     9841 acgcagactt tatccgtttg gctgatcagg taaaggagat ggagaaaacg cttctgcaaa
     9901 ctcctgacca gttgtccttt tttgaattgc tgaccttggt tgcttttctt tattttaggg
     9961 agcaggaggt ggatttggtt ttattagaag tgggaattgg tggcttactt gacacgacca
    10021 atgtggtaac tggagagctt gctgtcatca cctccattgg gcttgaccat caagaaacct
    10081 tgggtgatag tctagaagca attgcagagc agaaagctgg tattttcaag gctggtaaaa
    10141 aggcagtgat tgcgaaattg cctccagaag ctagacttgt ctgtcagaaa aaagccgaat
    10201 ctttagctgt tgacctttat caggcaggtc aagatttttc agtgctgaat ggtgattttt
    10261 caagctcttt actaaatctt tcacagctga acataggctt agaaggagcc tatcagcagg
    10321 agaatgcggc cttggcgttg caaacttttc ttctttttat gggagaaaga aaggaagcta
    10381 ttgatgaaca ggctgtaaga aaggccttgg aacagaccca ttgggctggt cgcttggagc
    10441 gtattcgccc acagatttac ttggatggtg ctcataacct ccctgccttg actcgcttgg
    10501 ttgagtttat caaagaaaaa gagcaggaag gctatcgacc tcaaatcctc tttggatcct
    10561 tgaaacgtaa ggattatcaa gggatgttgg gttatctgac tgaaaattta cctcaagtgg
    10621 aactcaaaat gaccggcttt gactatcaag ggtctttgga cgagacagat gtaacaggtt
    10681 accatgtaat tccctcttac cgagaattta tcagcagttt cgaagaaagg gcagacgctc
    10741 aagacttgct gttcgttaca gggtctctct attttatctc agaagtacgg ggctacctgc
    10801 tggaccgtga gcagataaat tgaccccctt ttttgaactt gttatactag gggaattgct
    10861 ggttagagaa aatttctcta aattggtagc agaaaggaaa ttcatcatga aattaaaaag
    10921 attcacactt tctcttgctt ctctagcaag ttttagtctc ttagtagctt gttcacaaag
    10981 agctcaacag gttcaacagc ctgttgctca gcaggtccaa cagcctgctc aacagaatac
    11041 caatactgca aatgcaggag gtaaccaaaa tcaagcggct ccagtacaaa accaacctgt
    11101 tgctcaaccg accgatattg atgggactta tactggtcag gatgacggag accgtatcac
    11161 tttagtggta actggaacga ctggtacatg gactgagctc gaatctgacg gggatcagaa
    11221 agtcaaacag gttacatttg attcagcaaa tcaacgcatg attattggcg atgatgtcaa
    11281 aatttacact gtaaacggta atcaaatcgt cgtagatgat atggatagag acccatcgga
    11341 ccaaatcgtt ttaactaaat aagactatgc aagtagaaag ggctggattt ttcagccttt
    11401 ttacttttac ctagaaaata atcataaata cttgccttct accgaatacc tgcgttatac
    11461 tagtatcaat tacttgtttt tagcattcaa actatcaagg aggggatatg aaatatagga
    11521 aatttcaatt attgatgtcc aagtatggct ttagtctttc gattatgcta cttgaacttt
    11581 gtcttgtttt tggtctcttt ctttatttag gacgcatggc tcccatttta tggattactg
    11641 tcctcattct actgagtatc atcacaatca tttcgatagt caaccgtaat acgactcctg
    11701 agaataaggt aacctggttg ttagtagcct ttgtgccagt atttggtccc ttgctctatc
    11761 tgatgtttgg tgaaaggcga ttgtccaaaa aagaaatcaa acaactgaag aagctaggct
    11821 ctatgcattt ccaagaagca aatagccagc tactaaaaga gaaattaaaa gaaagtgaca
    11881 aggcagctta tggagtcatc aagtccttat tgagtatgga taccaatgct gacatctatg
    11941 atcaaactgc ctctacattt tttcctaacg gagaagctat gtggaaaaag atggtagaag
    12001 atcttaaaaa ggctgagaaa tttattttct tggaatatta cattatagaa gaaggtttga
    12061 tgtggaatcg catactagat atactagagc aaaaggtagc tcagggtgta gaggttaaga
    12121 tgctctatga tgatatcggc tgtatggcta ctttaacagg agattatgca catcgacttc
    12181 gtcagctggg catcgaggcc cataaattca ataaagttat tcctcgtttg acagtggctt
    12241 ataataacag agatcataga aaaatattga ttgttgatgg tcagatagcc tatactggtg
    12301 gggtcaatct ggcagatgag tacattaacc acgtcgagag atttggttat tggaaggata
    12361 gtggaattcg cttagacgga ctagcagtaa aagccctgac acgcttattt ttgaccactt
    12421 ggtacattaa tcgaggagaa attagtgatt ttgatcaata tcatttagaa aatcattcta
    12481 tcccgagtga cggtttaacc attccatacg gaagtggacc caagccaatt tttcgagcgc
    12541 aggtagggaa aaaagtttat cagagtttaa tcaatcaagc aacagaatcg gtctatatta
    12601 cgacacctta tttgattata gattatgatt taacagagac aatcaaaaat gcagctatga
    12661 gaggggtcga tgttcgaatt atcacccctt acataccaga taagaagttc attcagttag
    12721 tcacgagagg agcttatccc gaccttcttt ctgctggtgt tcggatttat gagtatagtc
    12781 caggttttat tcatagtaag cagatgttgg tagacgaaga ttttgcggtg gtggggacaa
    12841 tcaatctcga ctaccggagc ttggtacacc attatgaaaa tgcagtctta ctctataaaa
    12901 ctccttctat aagggaaatc gcccgagatt ttcgaaatat atttgcagat tctcaggaag
    12961 tctatcctca ttctatcaaa acgagctggt atcaaaagct tgtaaaagaa atcgcccagc
    13021 tattcgcccc tatcttataa gaaatctaga acttaggata caaaccagcc ctatttttct
    13081 tgtcttttac taatagaaag aaagaggagg ggaattcatg ctaactaagg aagaagtgaa
    13141 tgatatgatt gagttcaaac agacacattt acaccgcttt cgggaaattg aaacctttgt
    13201 gaaactatgc aaaaaatcac tcaaacagcc atcgcaagct gacaatccta ggactttttg
    13261 atatactaag acagataaat tgaacaggag aaacgatatg agtgtgtatg gtagagtaga
    13321 agaagttcat aaggagaatc gtgaacctct agaataccaa atcgaacaag aatcgcatca
    13381 tcgtgaatca agtcgtcttc ctttggtgaa aattttacta tggagtacgc ttgtaacggg
    13441 gataactcta ggagtaccgc tattactcga cttaatgagt gcacaagaag tgcaggattt
    13501 ttatgcaggt tgggcgcttc atcagacagg gaagatttac agcgactatt atggaagtca
    13561 aggtttgctt tattatttgc tgacttacgt gagtcagggc ggatttttct ttgccatctt
    13621 tgagtggtta gccttggtag caggaggatt tttccttttt agatcagcgg acaccttgac
    13681 agagcaagga gaccaagctg gacatctggt gactattttt tacatgctag ttacaggtct
    13741 tgcttttggt ggaggctatg cgactctttt agcgcttcct ttcttattcg cagcctttag
    13801 tttagttgcg gcttacctaa gcaatccaag ccatgataag ggatttgtac ggattgggct
    13861 agctttggca ggcggatttt tctttgctcc cttatcatcg ctcctgttta ttgctgtagt
    13921 gggtttaggc ttgttggtct ttaaccttgg gcatagacgc tttgcgcatg ggttttatca
    13981 gtttcttgca gtggctttag gtttttcact tgtcttttat ccaactgcct actatagtgc
    14041 tgcaacagga agttttgggg atgcgattag tggtattcgt tatcctattg acagtattcg
    14101 ctttgatttt acttctaaaa ttttagagaa tatgtttttt tatggcttgt tgtcccttgg
    14161 tttgggattt gtggtttgta tctttttagg tctctttcaa tccaaaccct ttaaactata
    14221 cgtcatttcg gttcctgcaa gtcttgttgt aattttaggt ttgattttac ttttcttttc
    14281 acaagagcct ctgcacgctt cttatttgat ggtcgtcttc cctgttttcc tacttttatt
    14341 ggtaaccaat attaagagtc aacagagggg gcgtagtgct agaagaagcc gaagagaaac
    14401 gccagttagc ctatggagtc gtttcttcaa aggaaatcta tatctgctag tttttgggtt
    14461 tgtctatctt ttgtctgttc cttttttgat gaagtttgtc ctttatccag taccttatca
    14521 agaacgtaat cgtcttgctg atttggtaaa agaggagaca aatacggaag atgctatcta
    14581 tgcatgggat gatactgcga ctctttatcg taagagtgag cgcttgtcgc catcggcgat
    14641 tttgtccccg ttgcactata cagcaactga ggaaaatcgt aataagttac ttaatgactt
    14701 gaaagaaaaa caacctaagg tgattgtggt aaatgacaag gtggtagtct ggtctgaagt
    14761 ggaaacactt ttaaaagaaa attaccaaca agtaaagact gattactcag agtttaaagt
    14821 ctataaaatt aaataaccaa atcaatatct tgtgtatttt tagaaatttt aggattttta
    14881 acacaagata ttgatttttc tttttagagt ggtataatac tttttagaaa gaacatttta
    14941 gaaaagagca tgcgtatgat tgcactagaa gaaaaaatta caattttgcc aactctcttc
    15001 gtcgagaaac gagatgggag acgtgttgta tttgatgtgg acaagattga caaggctctc
    15061 cacaaggcgg ctgacaaggt tatggatgtg acacccctgg ttgaaaaacg cctcaatgat
    15121 ctgactgagc gaattattac agaaattcat agtcgctttc cacagggaat taagatttac
    15181 gaaattcaaa atatcgtaga acatgaactc cttgaagcca aagaatatgc gctggctgag
    15241 gagtatatta cttatcggac acagagggat tttgagcgct caaaagcgac ggatatcaac
    15301 tttagtattc ataaacttct caacaaagac cagacagttg tcaatgaaaa tgctaataaa
    15361 gacagtgatg tctttaacac tcagcgtgat ttgacagcag ggattgttgg gaaatcaatc
    15421 ggactgcaaa tgcttcctaa gcacgtagcc aatgcccacc aaaaggggga tatccactat
    15481 cacgatttgg actacagtcc ctatacccct atgaccaact gctgtttgat tgattttaag
    15541 ggtatgttgg aaaatggttt taagattgga aatgcagagg tagagagtcc caagtctatc
    15601 cagactgcga cagcacagat ttctcaaatc attgccaacg ttgcttctag ccagtacggt
    15661 ggctgttcag ctgaccgtat cgatgaaatt ttggcgcctt atgcagagaa gaactatcaa
    15721 aaacatctca aagatgcaga agagtgggta ttgcctgaaa aacgggaaga ttacgcttgg
    15781 aagaaaacgc aaaaggacat ctacgatgcc atgcaatctc ttgagtatga aatcaatact
    15841 ctcttcactt caaatggaca aacacctttt acttcgttag gttttggtct gggaaccagt
    15901 cgttttgaac gagaaattca aaaagctatt ttaaacattc gcatcaaggg tcttggttca
    15961 gaacaccgta cggctatctt tcctaaactt attttcactc tgaaaagagg ccttaattta
    16021 gaggaaggaa ctcccaacta cgacatcaag cagttggctc ttgagtgtgc aaccaaacgg
    16081 atgtatccag atgttttatc ttacgataag attgttgact tgacaggatc cttcaaggtg
    16141 cctatgggtt gccgttcttt cctccaagga tggaaggatg aaaatggtgt agaagtcaat
    16201 tcaggtcgca tgaatctggg tgttgtgacg gttaatctgc ctcgtattgc tcttgagtct
    16261 gaaggtgata tgaataagtt ctgggaaatc ttcaacgagc gcatgaatat cgcagaagat
    16321 gctcttgttt accgtgtcga acgcactaaa gaggcgacac cagcgaatgc tcctatcctt
    16381 tatcagtacg gtgcttttgg ccatcatcta ggtaaagaag aaagtgttga ccagctcttt
    16441 aagaatcgtc gtgcgaccgt ttcgctgggc tatatcggct tgtatgaagt agcgacagtt
    16501 ttctttggta acagctggga aagtaatcca gatgctaagg aattcacgct agacatcatt
    16561 cacgatatga aacgccgtgt agaagagtgg tcagaccaat atggctacca tttctctatc
    16621 tactcaacac catccgaaag tctgacagac cgtttctgcc gactagatat agacaagttt
    16681 ggctctattc ctgatatcac agacaaggaa tactacacca actctttcca ctacgatgtt
    16741 cgtaaaaatc caacaccgtt tgaaaaattg gactttgaga aagtctatcc agaagcaggt
    16801 gcgtcaggtg gtttcatcca ttattgtgag tatccagtcc ttcagcaaaa tccaaaggcc
    16861 ttggaagctg tctgggatta tgcttatgac cgtgtaggct atctaggcac caatactccg
    16921 attgaccgtt gctacaagtg tgactttgaa ggggattttg aaccaactga gagagggttt
    16981 gcttgtccaa actgtggcaa tagcgaccct aaaacagtag atgtggtgaa gcgaacttgt
    17041 ggctacctag gtaatcctca agcaagaccg atggtcaacg ggcgtcacaa ggaaatcgct
    17101 gcgcgtgtca aacatatgaa tggttcaacg attaaaatag ctgggcatca agtaacaaat
    17161 tagaaagaaa tgaaatggga aaatatcaac tagacgataa ggggcgcgca caagtgaccc
    17221 gttatcacga gaaacactct aaaggtggag ctggtaagaa agaacgcttg cttagcttca
    17281 gagaacaatt tttaaacaag aacaagaaaa aataaaagtg agagccagct ctcgcttttc
    17341 tcatagtggg aggtaaggat ggaattacgc agaccaagat tagcggataa gaaagctgtt
    17401 ttagatatga tgacagagtt tgaaaaattt cagtcgcctc acgacggcgg tttctgggat
    17461 acagagaact ttgtgtatga agactggtta gaaagcaatc aggaacagga aatgggaatt
    17521 aatctgcctg aaggatgggt tcctgcaatt cagttagtgg ctttttctga gaaaggtcaa
    17581 gcagttggat ttcttaatct ccggttgcgc ctcagtaact ttctactaga agaaggtggg
    17641 cacattggct actccatccg tccatctgaa agaggcaagg gttatgcaaa agagactctc
    17701 cgtcagggct tgcaagttgc taaggaaaag aacatcaaga aagctctggt gacctgtagc
    17761 gtgaataatc ctgctagcag agcagtcatt ctagcaaatg gtggaatatt tgaggatgct
    17821 cgcaatggag tcgagcgtta ttggatagag gtagcgaatg aataatccaa aaccacaaga
    17881 atggaaaagc gaggaactta gtcaaggtcg tatcattgac tacaaggcct ttaactttgt
    17941 tgatggcgaa ggcgtgcgta actctctcta tgtagcaggc tgtatgtttc actgcgaggg
    18001 atgttataat gttgcgactt ggtcttttaa tgctggcatt ccctatacag cagaattaga
    18061 agagcagatc atggcagatc ttggtcagcc ctatgttcaa ggtttgactt tgttgggagg
    18121 ggagcctttt ctcaatactg gcattctctt gccacttgtt aagcggattc ggaaagaatt
    18181 gtcagacaag gacatctggt cctggaccgg ctacacttgg gaagaaatga tgttggaaac
    18241 tccagataaa ctggaactct tgtcactgat tgatattctt gtcgatggaa gatatgatcg
    18301 aactaagaga aatcttatgc tccagtttcg aggttcgtct aaccaacgaa ttatcgatgt
    18361 gcaaaaatcg ctcaaaagtg ggcaagtagt gatttgggac aagctcaatg acggaaaaga
    18421 aagctatgaa caggtgaaga gagaatgaag aaaaaaggct tagtagacca actagtctca
    18481 gagatcgaga cggggaaagt caggacactg ggaatatacg gtcatggagc ttcaggtaaa
    18541 tcaacctttg cacaggaatt gtcccaagct ttagattcta ctacagtaaa tttgctagaa
    18601 acagatccct atatcacctc cggacgctat ttggtagtac ccaaggacgc gccgaatcaa
    18661 aaggtgacag ccagtctgcc agtggcgtat gaactggaga gtttgcagag agatatcttt
    18721 gccttgcagg cgggtatgga tgtcttgaca attgaagaac cttggaaggc tagtgaagtc
    18781 ttgtctggag ccaaaccaat tttgattgtc gaagggatgt ctgttggttt tctacccaag
    18841 gaactctttg aaaaaaccat ctgtttctac acggatgagg agaccgaatt aaagcgacgc
    18901 cttgctagag atacgactgt gagaaatcgc gatgcatcct ttatattggc tagccatcag
    18961 atgagacggg agcagtatct gcgatactat aaagaaactg agtctaaggc ggatatctta
    19021 gtggaccaat cagaagataa atttgatgtc aagaggactt aacttatgta tagaagaaaa
    19081 tccctaattt tagaaagaaa aagtaggaaa acagtttcat ctcaaaaaaa cgaaaaaacc
    19141 taacaaatcc cttgcaatcg caggggattt gtgttattct attatggtgc tgtaaattac
    19201 agccttagtt ttgatgcaag aggttgcgac acgctcggtt gcattgccac gcaacgccgc
    19261 gtcggttttc ttgtggagct agcctattat cttaaataga cgaaaaggag aaaaagatgg
    19321 caaacaaaaa aatccgtatc cgtttgaaag cttacgaaca ccgtacgctt gacacagcgg
    19381 ctgcaaaaat cgtagaatca gctactcgta caggtgcaca agttgcgggt ccaatcccac
    19441 ttccaactga acgtagcctc tacacaatca ttcgtgcgac tcacaaatac aaagactctc
    19501 gcgaacaatt tgaaatgcgt acacacaaac gtttgatcga tatcgttaac ccaactcaaa
    19561 aaacagttga tgccttgatg aaattggatc ttccaagtgg tgtaaacgta gaaatcaaac
    19621 tttaatctaa aatataaaag agcagaggct ggtgtttcaa tctaattgaa cacgggctaa
    19681 actcggtgtg aaaaagataa acttcctcgc gtttgctaga cactgcgtta gtttcctatt
    19741 ttcactttga gtttgacgcc ctttgtatct tagacttgag cataaaaaac gctcgttaaa
    19801 aactttttga ataaaaaata tagaaaagga actattttct catgacaaaa ggaatcttag
    19861 ggaaaaaagt gggaatgact caaatcttca ctgaagctgg cgaattgatc cctgtaacag
    19921 ttattgaagc aactccaaac gttgttcttc aagttaaaac tgttgaaaca gacggataca
    19981 acgctatcca agttggtttc gatgacaaac gcgaagtatt gagcaacaaa cctgctaaag
    20041 gacatgtagc gaaagctaac acggctccta agcgcttcat tcgtgaattc aaaaacgttg
    20101 aaggcttgga agttggtgct gaaattacag ttgaaacatt cgcagctgga gacgttgttg
    20161 acgtaactgg tacttctaaa ggtaaaggtt tccaaggtgt tatcaaacgc cacggacaat
    20221 cacgtggacc aatggctcac ggttctcgtt accaccgtcg tccaggttct atgggacctg
    20281 ttgcacctaa ccgcgtattc aaaggtaaaa accttgcagg gcgtatgggt ggcgaccgcg
    20341 taacaattca aaaccttgaa gttgtacaag ttgttccaga aaagaacgtt atccttatca
    20401 aaggtaacgt accaggtgct aagaaatctc ttatcactat caaatcagca gttaaagctg
    20461 gtaaataata aagaaagggg aaatcagtca caatggcaaa cgtaacatta tttgaccaaa
    20521 ctggtaaaga agctggccaa gttgttctta gcgatgcagt atttggtatc gaaccaaatg
    20581 aatcagttgt gtttgatgta atcatcagcc aacgcgcaag ccttcgtcaa ggaacacacg
    20641 ctgttaaaaa ccgctctgca gtatcaggtg gtggacgcaa accatggcgt caaaaaggaa
    20701 ctggacgtgc tcgtcaaggt tctatccgct caccacaatg gcgtggtggt ggtgttgtct
    20761 tcggaccaac tccacgttca tacggctaca aacttccaca aaaagttcgt cgcctagctc
    20821 ttaaatcagt ttactctgaa aaagttgctg aaaacaaatt cgtagctgta gacgctcttt
    20881 catttacagc tccaaaaact gctgaatttg caaaagttct tgcagcattg agcatcgatt
    20941 ctaaagttct tgttatcctt gaagaaggaa atgaattcgc agctctttca gctcgtaacc
    21001 ttccaaacgt gaaagttgca actgctacaa ctgcaagtgt tcttgacatc gcaaatagcg
    21061 acaaacttct tgtcacacaa gcagctatct ctaaaatcga ggaggttctt gcataatgaa
    21121 tttgtatgat gttatcaaaa aacctgtcat cactgaaagc tcaatggctc aacttgaagc
    21181 aggaaaatat gtatttgaag ttgacactcg tgcacacaaa cttttgatca agcaagctgt
    21241 tgaagctgct ttcgaaggtg ttaaagttgc caatgttaac acaatcaacg taaaaccaaa
    21301 agctaaacgt gttggacgtt acactggttt tactaacaaa actaaaaaag ctatcatcac
    21361 acttacagct gattctaaag caatcgagtt gtttgctgct gaagctgaat aatctaagga
    21421 ggaaatatcg tgggaattcg tgtttataaa ccaacaacaa acggtcgccg taatatgact
    21481 tctttggatt tcgctgaaat cacaacaagc actcctgaaa aatcattgct tgttgcattg
    21541 aagagcaagg ctggtcgtaa caacaacggt cgtatcacag ttcgtcacca aggtggtgga
    21601 cacaaacgtt tctaccgttt ggttgacttc aaacgtaata aagacaacgt tgaagcagtt
    21661 gttaaaacaa tcgagtacga tccaaaccgt tctgcaaaca tcgctcttgt acactacact
    21721 gacggtgtga aagcatacat catcgctcca aaaggtcttg aagtaggtca acgtatcgtt
    21781 tcaggtccag aagcagatat caaagtcgga aacgctcttc cacttgctaa catcccagtt
    21841 ggtactttga ttcacaacat cgagttgaaa ccaggtcgtg gtggtgaatt ggtacgtgct
    21901 gctggtgcat ctgctcaagt attgggttct gaaggtaaat acgttcttgt tcgtcttcaa
    21961 tcaggtgaag ttcgtatgat tcttggaact tgccgtgcta cagttggtgt tgtcggaaac
    22021 gaacaacatg gacttgtaaa ccttggtaaa gcaggacgta gccgttggaa aggtatccgc
    22081 ccaacagttc gtggttctgt aatgaaccct aacgatcacc cacacggtgg tggtgaaggt
    22141 aaagcaccag ttggtcgtaa agcaccatct actccatggg gcaaacctgc tcttggtctt
    22201 aaaactcgta acaagaaagc gaaatctgac aaacttatcg ttcgtcgtcg caacgagaaa
    22261 taatattaaa ctagtcgctt aagcaactag taaatccgcc agctcggtag cgctccatag
    22321 gagtgcaagc cgctgtggta caacatttaa aggagaaaat ataaaaatgg gacgcagtct
    22381 taaaaaagga cctttcgtcg atgagcattt gatgaaaaaa gttgaagctc aagctaacga
    22441 cgaaaagaaa aaagttatta aaacttggtc acgtcgttca acgatcttcc caagtttcat
    22501 tggttacact attgcagttt atgacggacg taaacacgta cctgtttaca tccaagaaga
    22561 catggtaggc cacaaacttg gtgaatttgc accaactcgt acttacaaag gtcacgctgc
    22621 agacgacaag aaaacacgta gaaaataagg agaacataaa tggcagaaat tacttcagct
    22681 aaagcaatgg ctcgtacagt acgtgtttca cctcgtaaat cacgtcttgt tcttgataac
    22741 atccgtggta aaagcgtagc cgatgcaatc gcaatcttga cattcactcc aaacaaagct
    22801 gctgaaatca tcttgaaagt tttgaactca gctgtagcta acgctgaaaa caactttggt
    22861 ttggataaag ctaacttggt agtatctgaa gcattcgcaa acgaaggacc aactatgaaa
    22921 cgtttccgtc cacgtgcgaa aggttcagct tcaccaatca acaaacgtac agctcacatc
    22981 actgtagctg ttgcagaaaa ataaggaggt aaaatcgtgg gtcaaaaagt acatccaatt
    23041 ggtatgcgtg tcggcatcat ccgtgattgg gatgccaaat ggtatgctga aaaagaatac
    23101 gcggattacc ttcatgaaga tcttgcaatc cgtaaattcg ttcaaaaaga acttgctgac
    23161 gcagcagttt caactattga aatcgaacgc gcagtaaaca aagttaacgt ttcacttcac
    23221 actgctaaac caggtatggt tatcggtaaa ggtggtgcta acgttgatgc actccgtgca
    23281 aaacttaaca aattgactgg aaaacaagta cacatcaaca tcatcgaaat caaacaacct
    23341 gatttggatg ctcaccttgt aggtgaagga attgctcgtc aattggagca acgtgttgct
    23401 ttccgtcgtg cacaaaaaca agcaatccaa cgtgcaatgc gtgctggagc taaaggaatc
    23461 aaaactcaag tatcaggtcg tttgaacggt gcagatatcg cccgtgctga aggatactct
    23521 gaaggaactg ttccgcttca cacacttcgt gcagatatcg attacgcttg ggaagaagca
    23581 gatactacat acggtaaact tggtgttaaa gtatggatct accgtggtga agttcttcca
    23641 gctcgtaaaa acactaaagg aggtaaataa ccaatgttag tacctaaacg tgttaaacac
    23701 cgtcgtgagt tccgtggaaa aatgcgcggt gaagcaaaag gtggaaaaga agtagcattc
    23761 ggtgaatacg gtcttcaagc tacaactagc cactggatca ctaaccgcca aatcgaagct
    23821 gctcgtatcg ccatgactcg ttacatgaaa cgtggtggta aagtttggat taaaatcttc
    23881 ccacacaaat catacactgc taaagctatc ggtgtgcgta tgggatctgg taaaggggca
    23941 cctgaaggtt gggtagcacc agttaaacgt ggtaaagtga tgttcgaaat cgctggtgta
    24001 tctgaagaga ttgcacgtga agcgcttcga cttgctagcc acaaattgcc agttaaatgt
    24061 aaattcgtaa aacgtgaagc agaataagga gaaggcatga aacttaatga agtaaaagaa
    24121 tttgttaaag aacttcgtgg tctttctcaa gaagaactcg cgaagcgcga aaacgaattg
    24181 aaaaaagaat tgtttgaact tcgtttccaa gctgctactg gtcaattgga acaaacagct
    24241 cgcttgaaag aagttaaaaa acaaatcgct cgtatcaaaa cagttcaatc tgaagcgaaa
    24301 taatagacta gggaaggaga aatttcaatg gaacgcaata atcgtaaagt tcttgttgga
    24361 cgtgttgtat ctgacaaaat ggacaagaca atcacagttg tagttgaaac aaaacgtaac
    24421 cacccagtct atggtaaacg tattaactac tctaaaaaat acaaagctca tgatgaaaac
    24481 aatgttgcca aagaaggcga tatcgtacgt atcatggaaa ctcgcccgct ttcagctaca
    24541 aaacgtttcc gtcttgtaga agttgttgaa gaagcggtca tcatctaatc aaacctgaaa
    24601 ggagaaaact gaaatgattc aaacagaaac tcgtttgaaa gtcgcagaca acagcggtgc
    24661 tcgcgaaatc ttgactatca aagttcttgg tggttcagga cgtaaatttg caaacatcgg
    24721 tgatgttatc gtggcatctg taaaacaagc tactcctggt ggtgcggtta aaaaaggtga
    24781 cgttgttaaa gcagttatcg ttcgtactaa atcaggtgct cgtcgtgctg atggttcata
    24841 catcaaattt gacgaaaacg cagcagttat catccgtgaa gacaaaactc ctcgcggaac
    24901 acgtatcttt ggcccagttg cacgtgaatt gcgtgaaggt ggcttcatga agatcgtgtc
    24961 acttgctcca gaagtacttt aatttttagg aacaaactag tcccctagct tcaagctagg
    25021 gtgcccttat gggcgtaaga aaaatcaagg agaaacctaa tgtttgtaaa aaaaggcgac
    25081 aaagttcgcg taatcgctgg taaagacaag ggaacagaag ctgttgtcct tactgccctt
    25141 ccaaaagtaa acaaagttat cgttgaaggt gttaacattg ttaagaaaca ccaacgtcca
    25201 actaacgagc ttcctcaagg tggtatcatc gagaaagaag cagctatcca cgtatcaaac
    25261 gttcaagttt tggacaaaaa tggtgtagct ggtcgtgttg gatacaaatt tgtagacggt
    25321 aaaaaagttc gctacaacaa aaaatcaggc gaagtgcttg attaatcacg aaggaaagga
    25381 gaagtataat ggcaaatcgt ttaaaagaaa aatatcttaa tgaagtagtt cctgctttga
    25441 cagaacaatt caactactca tcagtgatgg ctgtgcctaa agtagataag attgttttga
    25501 acatgggtgt tggtgaagct gtatcaaacg ctaaaagcct tgaaaaagct gctgaagaat
    25561 tggcacttat ctcaggtcaa aaaccactta tcactaaagc taaaaaatca atcgccggct
    25621 tccgtcttcg tgaaggtgtt gcgatcggtg caaaagttac ccttcgtggt gaacgtatgt
    25681 acgaattctt ggataaattg gtatcagttt cacttccacg tgtacgtgac ttccacggtg
    25741 tcccaacaaa atcatttgat ggacgcggga actacacact tggtgtgaaa gaacaattaa
    25801 tcttcccaga aatcaacttc gatgacgttg acaaaactcg tggtcttgac atcgttatcg
    25861 taacaactgc taacactgac gaagagtcac gtgcattgct tacaggcctt ggaatgcctt
    25921 ttgcaaaata atataggagg taaatctaat ggctaaaaaa tcaatggtag ctagagaggc
    25981 taaacgccaa aaaattgttg accgttatgc tgaaaaacgt gctgcattaa aggcggcagg
    26041 ggactacgaa ggtttatcta aattacctcg caacgcctca ccgactcgtt tacataatcg
    26101 ttgtagagtt acggggcgcc cacattcagt ttaccgcaaa tttggtctga gtcgtatcgc
    26161 ttttcgcgaa cttgcgcata aaggtcaaat tcctggtgta acaaaagcat cttggtaatt
    26221 taagatatca agggtgtcaa aactccaagt aaaaatagga aacttgacga agaaactaaa
    26281 gtttctagga aagtttatct ttttcacaca gagtttagcc cgggttcaat tgggcttgcc
    26341 aatttgaacc cgagctacag ctttggcaaa aaagaccaat ttgctttgga gcattgcttc
    26401 tgcattaaat tgtctatttt tgctcgtgct gttacgctct ttgtatcatg tattaactag
    26461 caagtgcaac ttgcaaacta ctagtaagag gagaaaaaca aaatggttat gactgaccca
    26521 atcgcagact tcctaactcg tattcgtaat gctaaccaag ctaaacacga agtacttgaa
    26581 gtacctgcat caaacatcaa aaaagggatt gctgaaatcc ttaaacgcga aggttttgta
    26641 aaaaacgttg aaatcattga agatgacaaa caaggcgtca tccgtgtatt tcttaaatac
    26701 ggaccaaatg gtgagaaagt tatcactaac ttgaaacgtg tttctaaacc aggacttcgt
    26761 gtctacaaaa aacgtgaaga ccttccaaaa gttcttaacg gacttggaat tgccatcctt
    26821 tcaacttctg aaggtttgct tactgataaa gaagcacgcc aaaagaatgt tggtggtgag
    26881 gttatcgctt acgtttggta aaatcaagat acaaagctcg taaagaacaa agcaaaatta
    26941 ggaagttgga gaagtttgtt tacaaacaag ccaacttatc tattttgcac agttcttaga
    27001 gcgtgttcag ttcagctctt gaactaaata agtatctgaa ccccgtgaaa actggccgtt
    27061 ctggcctgac aatttaacag gagaaaataa acatgtcacg tattggtaat aaagttatcg
    27121 tgttgcctgc tggtgttgaa ctcgctaaca atgacaacgt tgtaactgta aaaggaccta
    27181 aaggagaact tactcgtgag ttctcaaaag atattgaaat ccgtgtggaa ggtactgaag
    27241 taactcttca ccgtccaaac gattcaaaag aaatgaaaac tatccacgga actactcgtg
    27301 cccttttgaa caacatggtt gttggtgtat cagaaggatt caagaaagaa cttgaaatgc
    27361 gtggggttgg ttaccgtgca cagcttcaag gatctaaact tgttttggct gttggtaaat
    27421 ctcatccaga cgaagttgaa gctccagaag gaattacttt tgaacttcca aacccaacaa
    27481 caatcgttgt tagcggaatt tcaaaagaag tagttggtca aacagctgct tacgtacgta
    27541 gccttcgttc accagaacca tataaaggta aaggtatccg ttacgttggt gaattcgttc
    27601 gccgtaaaga aggtaaaaca ggtaaataat gttgagtggt tgatcatcaa ccaccaacct
    27661 attttccaac tttgtgcata gcacacgatt taaaactaaa gaggtgaaaa ctgtgatttc
    27721 aaaaccagat aaaaacaaac tccgccaaaa acgtcaccgt cgcgttcgcg gaaaactctc
    27781 tggaactgct gatcgcccac gtttgaacgt attccgttct aatacaggca tctacgctca
    27841 agtgattgat gacgtagcgg gtgtaacgct cgcaagtgct tcaactcttg ataaagaagt
    27901 ttcaaaagga actaaaactg aacaagccgt tgctgtcggt aaactcgttg cagaacgtgc
    27961 aaacgctaaa ggtatttcag aagtggtgtt cgaccgcggt ggatatctat atcacggacg
    28021 tgtgaaagct ttggctgatg cagctcgtga aaacggattg aaattctaat aggaggacac
    28081 tagaaaatgg catttaaaga caatgcagtt gaattagaag aacgcgtagt tgctgtcaac
    28141 cgtgttacaa aagttgttaa aggtggacgt cgtcttcgtt tcgcagctct tgttgttgtt
    28201 ggtgaccaca atggtcgcgt aggatttggt actggtaaag ctcaagaagt tccagaagca
    28261 atccgtaaag cagtagatga tgctaagaaa aacttgatcg aagttcctat ggttggaaca
    28321 acaatcccac acgaagttct ttcagaattc ggtggagcta aagtattgtt gaaacctgct
    28381 gtagaaggtt ctggagttgc cgctggtggt gcagttcgtg ccgttgtgga attggcaggt
    28441 gtggcagata ttacatctaa atcacttggt tctaacactc caatcaacat tgttcgtgca
    28501 actgttgaag gtttgaaaca attgaaacgc gctgaagaaa ttgctgccct tcgtggtatt
    28561 tcagtttctg atttggcata agaaagggga taaaatggct caaattaaaa ttactttgac
    28621 taagtctcca atcggacgca ttccatcaca acgtaaaact gttgtagcac ttggacttgg
    28681 caaattgaac agctctgtta ttaaagaaga taacgctgct atccgtggta tgatcacagc
    28741 agtatctcac ttagtaacag ttgaagaagt aaactaatga agttttaggg gatgtgcact
    28801 gtaccatccc ctaaaactag atatagtcat ctatgatgac atcgtatagg cgagttgatg
    28861 ggggagacaa ccttttctcc cttatcggcg ctagcatttt acaaaagagg agaaaataaa
    28921 aatgaaactt catgaattga aacctgcaga aggttctcgt aaagtacgta accgcgttgg
    28981 tcgtggtact tcatcaggta acggtaaaac atctggtcgt ggtcaaaaag gtcaaaaagc
    29041 tcgtagcggt ggcggagttc gccttggttt tgaaggtgga caaactccat tgttccgtcg
    29101 tcttccaaaa cgtggattca ctaacatcaa cgctaaagaa tacgcaattg tgaaccttga
    29161 ccaattgaac gtctttgaag atggtgctga agtaactcca gttgttctta tcgaagcagg
    29221 aattgttaaa gctgaaaagt caggtattaa aattcttggt aacggtgagt tgactaagaa
    29281 attgactgtg aaagcagcta aattctctaa atcagctgaa gaagctatca ctgctaaagg
    29341 tggttcagta gaagtcatct aagagaggtg acctatgttt tttaaattat taagagaagc
    29401 tcttaaagtc aagcaggttc gatcaaaaat tttatttaca atttttatcg ttttggtctt
    29461 tcgtatcgga actagcatta cagttcctgg tgtgaatgcc aatagcttga atgctttaag
    29521 tggattatcc ttcttaaaca tgttgagctt ggtgtcgggg aatgccctaa aaaacttttc
    29581 gatttttgcc ctaggagtta gtccctatat caccgcttct attgttgtcc aactcttgca
    29641 aatggatatt ttacccaagt ttgtagagtg gggtaaacaa ggggaagtag gtcgaagaaa
    29701 attgaatcaa gctactcgtt atattgctct agttctcgct tttgtgcaat ctatcgggat
    29761 tacagctggt tttaatacct tggctggagc tcaattgatt aaaactgctt taactccaca
    29821 agtttttctg acgattggta tcatcttaac agctggtagt atgattgtca cttggttggg
    29881 tgagcaaatt acagataagg gatacggaaa cggtgtttcc atgattatct ttgccgggat
    29941 tgtttcctca attccagaga tgattcaggg catctatgtg gactactttg tgaacgtccc
    30001 aagtagccgt atcacttcat ctatcatttt cgtaatcatt ttgattatta ctgtattgtt
    30061 gattatttac tttacaactt atgttcaaca agcagaatac aaaattccaa tccaatatac
    30121 taaggttgca caaggtgctc catctagctc ttaccttccg ttaaaggtaa accctgctgg
    30181 agttatccct gttatctttg ccagttcgat tactgcagcg cctgcggcta ttcttcagtt
    30241 tttgagtgcc acaggtcatg attgggcttg ggtaagggta gcacaagaga tgttggcaac
    30301 tacttctcca actggtattg ccatgtatgc tttgttgatt attctcttta cattcttcta
    30361 tacgtttgta cagattaatc ctgaaaaagc agcagagagc ctacaaaaga gtggtgccta
    30421 tatccatgga gttcgtcctg gtaaaggtac agaagaatat atgtctaaac ttcttcgtcg
    30481 tcttgcaact gttggttccc tcttccttgg tgtgatttcc attttaccga ttgcagctaa
    30541 agatgtattt ggtctttctg atgttgttgc ctttggtgga acaagtctct tgatcattat
    30601 ctctacaggt atcgaaggaa tcaagcaatt ggaaggttac ctattgaaac gtaagtatgt
    30661 tggtttcatg gacagaacag aataaaagta tttactgaat cagtaaatac tgagggagtg
    30721 gaggtttaaa ctctgacatt tgtaagagtt ggatctcccc tcttctattt tgtttttaaa
    30781 taggggtgaa aagacttttt gcttctattt aaaaataaaa taaggagatc aaatcatgaa
    30841 tcttttgatt atgggcttac ctggtgcagg taagggaact caagcagcaa aaatcgtaga
    30901 acaattccat gttgcacata tctcaacagg tgatatgttc cgcgctgcaa tggcaaatca
    30961 aactgaaatg ggtgttcttg ctaagtcata tattgacaag ggtgaattgg ttcctgacga
    31021 agttacaaat ggaatcgtaa aagaacgcct ttcacaagat gatattaaag aaacaggatt
    31081 cttattggat ggttacccac gtacaattga acaagctcat gccttggaca aaacattggc
    31141 tgaacttggc attgaactag aaggtattat caatattgaa gtgaaccctg acagcctctt
    31201 ggaacgtttg agtggccgta tcatccaccg cgtaactgga gaaactttcc acaaggtctt
    31261 taacccacca gttgactata aagaagaaga ttactaccaa cgtgaagatg ataagcctga
    31321 gacagtaaaa cgtcgtttgg atgttaatat tgctcaagga gaaccaatca ttgctcacta
    31381 ccgtgccaaa ggtttggttc atgacatcga aggtaatcaa gatatcaatg atgtcttctc
    31441 agatattgaa aaagtattga caaatttgaa ataaagcgtt tttcgcactt gcaaaaatcc
    31501 gctacaaatg ttatactgag atagtctgac ttataattgt tgtctctgtg tctagaggca
    31561 tcgaatcgaa atttatggag gtgcttttgc gtggcaaaag acgatgtgat tgaagttgaa
    31621 ggcaaagtag ttgatacaat gccgaatgca atgtttacgg ttgaacttga aaatggacat
    31681 cagattttag caacagtttc tggtaaaatt cgtaaaaact atattcgtat tttagcggga
    31741 gatcgtgtta ctgtcgaaat gagtccatat gacttgacac gtggacgtat cacttaccgc
    31801 tttaaataat cgaaaaactt ggagggataa gaaatgaaag taagaccatc ggtcaaacca
    31861 atttgcgaat actgtaaagt tattcgtcgt aatggtcgtg ttatggtaat ttgcccagca
    31921 aatccaaaac acaaacaacg tcaaggataa gatagaaagg agaaaacatg gctcgtattg
    31981 ctggagttga tattccaaat gacaaacgcg tagtaatctc attgacttat gtttatggta
    32041 tcggacttgc aacatctaag aaaattttgg ctgctgctgg aatctcagaa gatgttcgtg
    32101 tacgtgatct tacatcagat caagaagatg ctatccgtcg tgaagtggat gcaatcaaag
    32161 ttgaaggtga ccttcgtcgt gaagtaaact tgaacatcaa acgtttgatg gaaatcggtt
    32221 cataccgtgg tatccgtcac cgtcgtggac ttcctgtccg tggacaaaac actaaaaaca
    32281 acgctcgcac tcgtaaaggt aaagctgttg cgattgctgg taagaaaaaa taatatagga
    32341 ggtaaaagtc ttggctaaac caacacgtaa acgtcgtgtg aaaaagaata tcgaatctgg
    32401 tattgctcat attcacgcta catttaataa cactattgtt atgattactg atgtgcatgg
    32461 taatgcaatt gcttggtcat cagctggtgc tcttggtttc aaaggttctc gtaaatctac
    32521 accattcgct gctcaaatgg cttctgaagc tgctgctaaa tctgcacaag aacacggtct
    32581 taaatcagtt gaagttactg taaaaggtcc aggttctggt cgtgagtcag ctattcgtgc
    32641 gcttgctgcc gctggtcttg aagtaacagc aattcgtgat gtgactccag tgccacacaa
    32701 tggtgctcgt cctccaaaac gtcgccgtgt ataatcatcg cattacactg cttttcgttt
    32761 aagagggagt aactaaatga tcgagtttga aaaaccaaat ataacaaaaa ttgatgaaaa
    32821 taaagattat ggcaagtttg taatcgaacc acttgaacgt ggctacggta caactcttgg
    32881 taactctctt cgtcgtgtac ttctagcttc tctaccagga gcagctgtga catctatcaa
    32941 cattgatggt gtgttacatg agtttgacac agttccaggt gttcgtgaag acgtgatgca
    33001 aatcattctg aacattaaag gaattgcagt gaaatcgtac gttgaagacg aaaaaatcat
    33061 cgaactggat gttgaaggtc ctgctgaagt aacagctggt gacattttga cagatagcga
    33121 tattgaaatt gtaaatccag atcattatct ctttacaatc ggtgaaggtt cttctctaaa
    33181 agcgactatg actgttaaca gtggtcgtgg atatgtacct gctgatgaaa ataaaaagga
    33241 taatgcacca gttggaacac ttgctgtaga ttctatttat acaccagtta caaaagtcaa
    33301 ctatcaagtg gaacctgctc gtgtaggtag caatgatggt ttcgacaaat taacccttga
    33361 aatcttgaca aatggaacaa ttattccaga agatgcttta gggctttcag cacgtatttt
    33421 gacagaacat cttgatttgt ttacaaatct tactgagatt gctaagtcaa ctgaagtgat
    33481 gaaagaagct gatactgaat ctgacgaccg tattttagat cgtacgattg aggaactgga
    33541 cttgtctgtg cgttcataca actgtttaaa acgtgccggt atcaatactg tgcatgattt
    33601 gacagaaaaa tctgaagcag agatgatgaa agtacgaaat cttggacgca agagtttgga
    33661 agaagtgaaa ctcaaactca ttgatttggg tcttggatta aaagataaat aaaggaggaa
    33721 tacatggctt accgtaaact aggacgcact agctcacaac gtaaagcaat gcttcgcgat
    33781 ttgacaactg accttttgat caacgaatca atcgtgacaa ctgaagctcg tgctaaagaa
    33841 atccgtaaaa ctgttgaaaa aatgattact ctaggtaaac gtggtgattt gcatgcacgt
    33901 cgtcaagcag ctgctttcgt acgtaatgaa atcgcatctg aaaactatga tgaagcaact
    33961 gataagtaca cttctactac agcacttcaa aaattgttct cagaaatcgc acctcgttat
    34021 gctgaacgta acggtggata cactcgtatc cttaaaactg aaccacgtcg tggtgatgca
    34081 gcgccaatgg cgatcatcga attagtataa aatcatcaat tttgttgagt gttatgatga
    34141 tggagtcttg tgctcttagt ctagctctgg tctaccgcta ggatttcggt cctagcggga
    34201 acactcatca taagttggga tagtagacgc ttgtttacga aattgttttt ttcttaagaa
    34261 caacttcgta agcaggcgtt tttgagtatt ttcgttagaa ttatgctata ctatttgaaa
    34321 agaatcctgt ttaatgttaa ggtttcttat tttaagaaga attggagttt acttatgaaa
    34381 gccattataa ctgttgttgg taaagataaa tctggaattg ttgcaggtgt ttctggtaaa
    34441 attgcagaat taggattgaa tattgacgat atctctcaaa ctgtcttgga tgaatatttt
    34501 acgatgatgg ctgttgtatc tagtgatgaa aagcaagatt ttacctatct tcgtaatgaa
    34561 tttgaagctt ttgggcaaac tttgaatgta aaaatcaata ttcagagtgc agcgattttc
    34621 gaagctatgt ataatatcta ggaggtcatc atggatatta gacaagttac tgaaaccatc
    34681 gccatgattg aggagcaaaa cttcgatatt agaaccatta ccatggggat ttctcttttg
    34741 gactgtatcg atccagatat caatcgtgct gcggagaaaa tctatcaaaa aattacgaca
    34801 aaggcggcta atttagtagc tgttggtgat gaaattgcgg ctgagttggg aattcctatc
    34861 gttaataagc gtgtatcggt gacacctatt tctctgattg gggcagcgac agatgcgacg
    34921 gactacgtgg ttctggcaaa agcgcttgat aaggctgcga aagagattgg tgtggacttt
    34981 attggtggtt tttctgcctt agtacaaaaa ggttatcaaa agggagatga gattctcatc
    35041 aattccattc ctcgcgcttt ggctgagacg gataaggtct gctcgtcagt caatatcggc
    35101 tcaaccaagt ctggtattaa tatgacggct gtggcagata tgggacgaat tatcaaggaa
    35161 acagcaaatc tttcagatat gggagtggcc aagttggttg tattcgctaa tgctgttgag
    35221 gacaatccat ttatggcggg tgcctttcat ggtgttgggg aagcagatgt tatcatcaat
    35281 gtcggagttt ctggtcctgg tgtggtgaaa cgtgctttgg aaaaagttcg tggacagagc
    35341 tttgatgtag tagccgaaac agttaagaaa actgccttta aaatcactcg tatcggtcaa
    35401 ttggttggtc aaatggccag tgagagactg ggtgtggagt ttggtattgt ggacttgagt
    35461 ttggcaccaa cccctgcggt tggagactct gtggcacgtg tccttgagga aatggggcta
    35521 gaaacagttg gcacgcatgg aacgacagct gccttggccc tcttgaacga ccaagttaaa
    35581 aagggtggag tgatggcctg taaccaggtc ggtggtctat ctggtgcctt tatccctgtt
    35641 tctgaggatg aaggaatgat tgctgcagtg caaaatggct ctcttaattt agaaaaacta
    35701 gaagctatga cggctatctg ttctgttgga ttggatatga ttgccatccc agaagatacg
    35761 cctgctgaaa ccattgcggc tatgattgcg gatgaagcag caatcggtgt tatcaacatg
    35821 aaaacaacag ctgttcgtat cattcccaaa ggaagagaag gcgatatgat tgagtttggt
    35881 ggtctattag gaactgcacc cgttatgaag gttaatgggg cttcgtctgt cgacttcatc
    35941 tctcgcggtg gacaaatccc agcaccaatt catagtttta aaaattaaga aaataggaga
    36001 aattttaagt tctatttaag attagatgtg tatactataa tcattaaata aagacctcct
    36061 aatattattt gaaacagata acactgaatt agtttgaatt tgattttcat ctaatatctt
    36121 tatttaatga actcctaaac tttttcataa taatctcctt caaaagtcgc ctgtatgggt
    36181 ggcttttatt ttatcattca tgatataata gaagcaaacg gaggacggaa aatggtaaaa
    36241 gtacgattgt atttggtacg tcatggcaag accatgttta acacgattgg tcgcgcgcaa
    36301 ggttggagcg atactccctt aactgctgaa ggtgaacgag ggattcaaga gttaggaatc
    36361 ggtttgcgag aatctgatct acagtttgag cgcgcttatt cgagtgattc tggtcgtacc
    36421 attcagacca tgggaattat acttgaagaa cttggcttgc aggggaaaat cccttatcgc
    36481 atggacaagc gtatcagaga atggtgtttc ggtagttttg atggagccta tgatggcgat
    36541 cttttcatgg gcattattcc tcgtatcttt aatgtggacc acgttcacca attgtcttat
    36601 gctgaactgg ctgagggctt ggtagaggtc gatacagctg gttgggctga aggttgggaa
    36661 aaactcagtg gccgaatcaa ggaaggcttt gaaataattg caaaagaaat ggaagatcaa
    36721 ggtggaggta acgcccttgt tgtcagccat ggaatgacta ttggaaccat tgtttatctg
    36781 attaatggca tgcatccgca tggtctagat aatggtagcg tgacgattct tgaatatgag
    36841 gacggtcagt ttagggttga agttgtcggt gaccgtagtt accgagagct aggacgtgag
    36901 aagatggaag aaggttctat ttaatcagtc tagacttgct tgccatgagc tagggatttg
    36961 ataagaatat caagataaga aaaaacagcc gagggtactc ctttcggctg tttttgatgt
    37021 ggaaaactaa agtgtaatgc tattgctttt agagattttc ataaacaaga gcaaggaacc
    37081 tactgttaga acagtcagga tagttgacaa ggctgcggct acaccgtaat ttcctctgag
    37141 aatctctgta taaatagcca cagtcattgt tcttgttttg acattgtaga ggaggataga
    37201 agtagagagt tttgaaatca ttgtgaccca agataagatg gctccagaaa tgataccaga
    37261 tagcatcatt ggagttgtaa tcttagcaaa gatattgaga cgactacttc ctaagctttc
    37321 agcagcttct tcaatacttg gtgctatttg ttgtaagcta gcaacagatg agcgaatagt
    37381 ataaggtaat cttctgacag atagagacat aatcaagatg aaagcagtcc ctgtaatcat
    37441 aagaaatcca cttccaaata gaccagtatt gaaggaagaa atgaaggcaa tccctagaac
    37501 ggttcctggt acaatataag gtaccatact gaggctgtca attaagtttg taaacaaatt
    37561 ccgttttcta acggctaggt aggagataaa tgtcgcaaat agaacaacta gaactaaggc
    37621 aatcaaaggg atacgaatgg tattgaaaat agcagatccc atacgattga aagctacctt
    37681 gtaactgttt ggagaataac ctttaacaaa taccatacct gatgttttta ggaaagaggt
    37741 ataaattaag tagatttgag gtaaaacaga gataaagata attccgtaga ctgttgcata
    37801 aatggcagcc atttttcctt ttgtagtttt tttaggctca attggatgga gcagattcat
    37861 gctgaaactg tagcggtttg caatgtgttt ttggataagg aaaattgcca aggcaatgat
    37921 aatcgccata attgcaaaag cagaatttcc tccaacctcg ctaataaatt gggtataaat
    37981 caggacaggg aaagtccgat atccttcgcc aatcaacata ggcgttccaa agtctgagaa
    38041 tgctctcata aatacaagca aggagctgct agtaaggttg aaactaggag aggtaaaaca
    38101 accgttacga taggtttaaa tccgaaggac cccatgcttt cagctgcttc aagtagagaa
    38161 ttgtcaatac tgttcattgt tccagcaaca tatagaaata ccagtgggaa tagttgcagt
    38221 gtaaagacaa gtatacttcc tttgaatcca taaatatcga tagctggaag atgaagggca
    38281 tttgtcaaaa atttagtgat gacctcattt cgtctcagca agagaatcca ggagtaggct
    38341 cctacgaaag gagctgacat ggaagcaatg ataatcaata tttgtagaaa tttcttcccc
    38401 ttgaagtcat acatagagaa gagataagct aatagggttc ctacaactaa ggaagtgaca
    38461 gtagcggtaa tggaaaccct gaaactgttg actagtgtct cagagtagta ggttttacta
    38521 aagaaagtga caaaattagc tagtgagaat tgtccttcat gtataagtgc ttgcttgagc
    38581 acggtaacga taggataaac gagaaagaca agataggtaa gaaagatgaa gaaagaggaa
    38641 gctgtccaaa tatttagttt tttacattcc atggttgact ccttttatca ggttttggga
    38701 accatctgca gaaaagatgt ttaatttttg cgtattgatt cgtagacgaa tacgattgcc
    38761 tttttgtagt tcttcttcaa aagttgattc ttcactaact tgaatttttg aggcaaaacc
    38821 tgtctcaatg aaatactccg tatttagtcc aagatagacg ctatcgctaa tagttccttc
    38881 aatatctcca gattcatctt tgataaactc ttcgggacga atgcttacat gaatagcttg
    38941 ctcctcaacc tgatcaagag ctggcattcg aagggcatag ccatctgaaa agacgatata
    39001 agcgccgtcg ctccgttttt caagattggc agggataata tttgtgcgtc cgataaaggt
    39061 tgccacaaac tcattagctg gtttatgata gagttctttt ggtcggccga tttgttggat
    39121 caccccatct ttcataacag caatttggtc tgaaatagcc atggcttctt cttggtcgtg
    39181 ggttacataa acagttgtaa ttcccacttc gtgttggatt tctcggatgg cttgacgcat
    39241 atccaagcga agtttggcct ccagattact aagtggctcg tccatgagga gaacacttgg
    39301 attaaccgct aaggcgcatg ccaaggtgac acgttgttgt tgtccaccac tgagtttatc
    39361 gggttttcga tccgcatatt gagcaatttg catgagttca agatacttgt tggtctgttg
    39421 aatcaattct tcttttgcat aagaccaaaa gcaacgttgt ctcggacagt caaatgtggg
    39481 aaaatagcgt agttttggaa aaccatcccg atattgcgtt tgctgggttc catattattg
    39541 atttttgtat catcgaagta aaattctcaa tctttgatac tgttgaaacc tgcaatcata
    39601 cgaagaaggg tcgttttccc acatcctgaa gctccaagaa gggtaaagag acttcctttt
    39661 ggaattgtaa tgttcaaatt ctcaataaca gggacatcgt ggtagatttt tttggcgtta
    39721 ataattttga tctcactcat agtgaacctc ttttactgtt tagattggat atctgtaaag
    39781 acttcgttgt atttcttaac gatatctgat ttattcttga tgacataatc ataatcttca
    39841 gtgagtgttt tgattttgtc aattggtttc atgttttcgc ttgttttagc atttttacga
    39901 acaggacggt tagtagtggt tgtaccaagt gtatcttgta cttcttgaga gataataaaa
    39961 tcgataaatt tcttggcatt ttccatattt ttagattttt taacgatagc agcactagca
    40021 ggtaggaaga cggttccttc ttttggatag actaccttaa tgttagctcc gtcatttaag
    40081 agtttaactg ctggatcttc ataagagaga ccaacagtca tttctccatc agcgactgct
    40141 ttatagacac cagatgaact taaaccgatt ttatagta
//
LOCUS       DAVBDG010000014        37619 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBDG010000014 DAVBDG010000000
VERSION     DAVBDG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.014"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            650..2803
                     /locus_tag="VQ526_000668"
     CDS             650..2803
                     /locus_tag="VQ526_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2554421.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGEMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2816..4165
                     /locus_tag="VQ526_000669"
     CDS             2816..4165
                     /locus_tag="VQ526_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2554422.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSFYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4335..5042
                     /locus_tag="VQ526_000670"
     CDS             4335..5042
                     /locus_tag="VQ526_000670"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2554423.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5244..8969
                     /locus_tag="VQ526_000671"
     CDS             5244..8969
                     /locus_tag="VQ526_000671"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2554424.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDVTELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMNWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNSYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9062..10504
                     /locus_tag="VQ526_000672"
     CDS             9062..10504
                     /locus_tag="VQ526_000672"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2554425.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPSNLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10541..11563
                     /locus_tag="VQ526_000673"
     CDS             10541..11563
                     /locus_tag="VQ526_000673"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2554426.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTESAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11560..12105
                     /gene="purN"
                     /locus_tag="VQ526_000674"
     CDS             11560..12105
                     /gene="purN"
                     /locus_tag="VQ526_000674"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2554427.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYIKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYLEVVKALFTD"
     gene            12189..12698
                     /locus_tag="VQ526_000675"
     CDS             12189..12698
                     /locus_tag="VQ526_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2554428.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12723..14270
                     /gene="purH"
                     /locus_tag="VQ526_000676"
     CDS             12723..14270
                     /gene="purH"
                     /locus_tag="VQ526_000676"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2554429.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWEYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14392..15654
                     /gene="purD"
                     /locus_tag="VQ526_000677"
     CDS             14392..15654
                     /gene="purD"
                     /locus_tag="VQ526_000677"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2554430.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLDYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16057..16545
                     /gene="purE"
                     /locus_tag="VQ526_000678"
     CDS             16057..16545
                     /gene="purE"
                     /locus_tag="VQ526_000678"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2554431.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTILPVIGVPVNSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDQSIATALADFAEEQGKIAEESSNE
                     LI"
     gene            16532..17623
                     /gene="purK"
                     /locus_tag="VQ526_000679"
     CDS             16532..17623
                     /gene="purK"
                     /locus_tag="VQ526_000679"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2554432.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDVVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKIATGGYDGHGQNVIRSADD
                     LEEAYALADSAACVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPAIKLHAPAVMLNVLGQHVEAAEKYVTENPSTHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVEEFGEGIDF"
     gene            17633..17860
                     /locus_tag="VQ526_000680"
     CDS             17633..17860
                     /locus_tag="VQ526_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2554433.1"
                     /translation="MIQIIVNAFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            17923..19221
                     /gene="purB"
                     /locus_tag="VQ526_000681"
     CDS             17923..19221
                     /gene="purB"
                     /locus_tag="VQ526_000681"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2554434.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19276..23211)
                     /gene="strH"
                     /locus_tag="VQ526_000682"
     CDS             complement(19276..23211)
                     /gene="strH"
                     /locus_tag="VQ526_000682"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2554435.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTTTTENQ
                     PTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEVS
                     STPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDEA
                     KAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTDL
                     HLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTDL
                     INYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAVA
                     FTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYEK
                     FIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVASS
                     KLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIPI
                     IGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRYT
                     AESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKRE
                     VEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTIT
                     ANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSPG
                     HMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKIF
                     NFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYED
                     KDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGGF
                     LKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFAD
                     HNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTLV
                     ANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAGQ
                     ENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESGL
                     APTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADGK
                     EVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPAE
                     ESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23527..24243)
                     /locus_tag="VQ526_000683"
     CDS             complement(23527..24243)
                     /locus_tag="VQ526_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2554436.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24591..26378
                     /locus_tag="VQ526_000684"
     CDS             24591..26378
                     /locus_tag="VQ526_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2554437.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26375..26851
                     /locus_tag="VQ526_000685"
     CDS             26375..26851
                     /locus_tag="VQ526_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2554438.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26879..27784
                     /locus_tag="VQ526_000686"
     CDS             26879..27784
                     /locus_tag="VQ526_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2554439.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27771..28586
                     /locus_tag="VQ526_000687"
     CDS             27771..28586
                     /locus_tag="VQ526_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2554440.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28593..28997
                     /locus_tag="VQ526_000688"
     CDS             28593..28997
                     /locus_tag="VQ526_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2554441.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29296..30462
                     /locus_tag="VQ526_000689"
     CDS             29296..30462
                     /locus_tag="VQ526_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2554442.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30578..31615
                     /locus_tag="VQ526_000690"
     CDS             30578..31615
                     /locus_tag="VQ526_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2554443.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31823..32677
                     /locus_tag="VQ526_000691"
     CDS             31823..32677
                     /locus_tag="VQ526_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2554444.1"
                     /translation="MTYEYKSHIYLAETVLNIKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            32827..33084
                     /locus_tag="VQ526_000692"
     CDS             32827..33084
                     /locus_tag="VQ526_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2554445.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(33297..33380)
                     /locus_tag="VQ526_000693"
                     /pseudo
     CDS             complement(33297..33380)
                     /locus_tag="VQ526_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(33414..33506)
                     /locus_tag="VQ526_000694"
                     /pseudo
     CDS             complement(33414..33506)
                     /locus_tag="VQ526_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(33542..33838)
                     /locus_tag="VQ526_000695"
     CDS             complement(33542..33838)
                     /locus_tag="VQ526_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2554446.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(33828..33989)
                     /locus_tag="VQ526_000696"
     CDS             complement(33828..33989)
                     /locus_tag="VQ526_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2554447.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            34059..34823
                     /locus_tag="VQ526_000697"
     CDS             34059..34823
                     /locus_tag="VQ526_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2554448.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            35388..36767
                     /locus_tag="VQ526_000698"
     CDS             35388..36767
                     /locus_tag="VQ526_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2554449.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            36781..37446
                     /locus_tag="VQ526_000699"
     CDS             36781..37446
                     /locus_tag="VQ526_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2554450.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
BASE COUNT        10311 a         7307 c         8823 g        11178 t
ORIGIN      
        1 ttaattagaa aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa
       61 taatcaaaac tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaataggagt
      121 cttatagtaa cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct
      181 attctgacta ggaatagatc ataccagagg tagcttagaa atagcagaga cattagaaat
      241 tgaagtaata aataggatgt cgtaagtgtt actatcaatg atttatttgt ttcaagcttg
      301 tctagggtga cagtaaaaaa tcaatttcct ttcaatagca tatttttagt gggcagtact
      361 cttgttctgc ctattttttt atccaaaaag tgcagttggg agggagatag gctcatttgg
      421 gaaggaagtc cagtttttgt ttagtgattg gggtaagata gttgttatca gatgagttta
      481 tactcttcga aaatcaaatt caaaccacgt caacgtcgcc ttgccgtata tatgtgactg
      541 acttcgtcag tcctatctac aacctcaaaa cagtgttttg agcagcctgc ggctagtttc
      601 ctagtttgct ctttgatttt cattgagtat tagggaaaag gagatgaata tgaaatttgg
      661 gaaacgtcac tatcgtccgc aagtggatca gatggactgc ggtgtagctt cattagccat
      721 ggtttttggc tactatggta gttattattt tttggctcac ttgcgagaat tggctaagac
      781 gaccatggat gggacgacgg ctttgggctt ggtcaaggtg gcagaggaga ttggttttga
      841 gacgcgagcc attaaggcgg atatgacgct ttttgacttg ccggatttga cttttccttt
      901 tgttgcccat gtgcttaagg aagggaaatt gctccactac tatgtggtga ctgggcagga
      961 taaggatagc attcatattg ccgatccaga tcccggggtg aagttgacta aactgccacg
     1021 tgagcgtttt gaggaagaat ggacaggagt gactcttttt atggcaccta gtccagacta
     1081 taagccttat aaggaacaaa aaaatggtct gctctctttt atccctatat tagtgaagca
     1141 gcgtggcttg attgccaata tcgttttggc aacactcttg gtaaccggga ttaacattgt
     1201 gggttcttat tatctgcagt ctatcattga tacctatgtg ccagatcaga tgcgttcgac
     1261 actagggatt atttctattg ggctagtcat cgtctacatc ctccagcaaa tcttgtctta
     1321 cgctcaggag tatctcttgc ttgttttggg gcaacgcttg tcgattgacg tgattttgtc
     1381 ctatatcaag catgtttttc acctccctat gtccttcttt gcgacacgca ggacagggga
     1441 gatcgtgtct cgttttacag atgctaacag tatcatcgat gcgctggctt cgaccatcct
     1501 ttcgattttc ctagatgtgt caacggttgt cattatttcc cttgttctat tttcacaaaa
     1561 taccaatctc tttttcatga ctttattggc gcttcctatc tacacagtga ttatctttgc
     1621 ctttatgaag ccgtttgaaa agatgaatcg ggataccatg gaagccaatg cggttctgtc
     1681 ttcttctatc attgaggaca tcaacggtat tgagactatc aagtccttga ccagtgaaag
     1741 tcagcgttac caaaaaattg acaaggaatt tgtggattat ctgaagaaat cctttaccta
     1801 tagtcgagca gagagtcagc aaaaggctct gaaaaaggtt gcccatctct tgcttaatgt
     1861 cggcattctc tggatggggg ctgttctggt catggatggc aagatgagtt tggggcagtt
     1921 gattacctat aataccttgc tggtttactt taccaatcct ttggaaaata tcatcaatct
     1981 gcaaaccaag cttcagacag cgcaggttgc caataaccgt ctaaatgaag tgtatctagt
     2041 agcttctgag tttgaggaga agaaaacagt tgaggatttg agcttgatga agggagagat
     2101 gaccttcaag caggttcatt acaagtatgg ctatggtcga gacgtcttgt cggatatcaa
     2161 tttaaccgtt ccccaagggt ctaaggtggc ttttgtgggg atttcagggt caggtaagac
     2221 gactttggcc aagatgatgg ttaattttta cgacccaagt caaggggaga ttagtctggg
     2281 tggtgtcaat ctcaatcaga ttgataaaaa agccctgcgc cagtacatca actatctgcc
     2341 tcaacagccc tatgtcttta acggaacgat tttggagaat cttcttttgg gagccaagga
     2401 ggggacgaca caggaagata tcttacgggc ggtcgaattg gcagagattc gagaggatat
     2461 cgagcgcatg ccactgaatt accagacaga attgacttcg gatggggcag ggatttcagg
     2521 tggtcaacgt cagagaatcg ctttggcgcg tgctctcttg acagatgcgc cggtcttgat
     2581 tttggatgag gcgactagca gtttggatat tttgacagag aagcggattg tcgataatct
     2641 catggctttg gacaagacct tgattttcat tgctcaccgc ttgactattg ctgagcggac
     2701 agagaaggta gttgtcttgg atcagggcaa gattgtcgaa gaaggaaagc atgctgattt
     2761 gcttgcacag ggtggctttt acgcccattt ggtcaatagc tagaaagagg agaggatgaa
     2821 accagaattt ttagaaagtg cggagtttta taatcgtcgt taccataatt tttccagtag
     2881 tgtgattgta cccatggccc ttctgctcgt gtttttactt ggctttgcaa ctgttgcaga
     2941 gaaggagatg agtttgtcca ctagagctac tgtcgaacct agtcgtattc ttgcaaatat
     3001 ccagtcaact agcaacaatc gtattcttgt caatcatttg gaagaaaata agctggttaa
     3061 gaagggggat cttttggttc aataccaaga aggggcagag ggtgtccaag cggagtccta
     3121 tgccagtcag ttggacatgc taaaggatca aaaaaagcaa ttggagtatc tgcaaaagag
     3181 cctgcaagaa ggggagaacc actttccaga ggaggataag tttggctacc aagccacctt
     3241 tcgcgactac atcagtcaag caggcagtct tagggctagt acatcgcaac aaaatgagac
     3301 catcgcgtcc cagaatgcag cagctagcca aacccaagcc gaaatcggca acctcatcag
     3361 tcaaacagag gctaaaattc gcgattacca gacagctaag tcagctattg aaacaggtgc
     3421 ttccttggcc ggtcagaatc tagcctactc tttttaccag tcctacaagt ctcagggcga
     3481 ggaaaatccc caaactaagg ttcaggcagt tgcacaggtt gaagcacaga tttctcagtt
     3541 agaatctagt cttgctactt accgtgtcca gtatgcaggt tcaggtaccc agcaagccta
     3601 tgcgtcaggg ttaagcagtc aattggaatc ccttaaatcc caacacttgg caaaggttgg
     3661 tcaggaattg acccttctag cccagaaaat cttggaggca gagtcaggta agaaggtaca
     3721 gggaaatctt ttagacaagg ggaaaattac ggcgagtgag gatggggtgc ttcatcttaa
     3781 tcctgagacc agtgattcta gcatggttgc agaaggtgcc ctactagccc aactttatcc
     3841 atctttggaa agagaaggga aagccaaact cacagcctat ctaagttcaa aagatgtagc
     3901 aagaatcaag gtcggtgatt ctgttcgcta tactacgact catgatgccg ggaatcaact
     3961 tttcctagat tctactatta caagtattga tgcgacagct actaagactg agaaagggaa
     4021 tttctttaaa atcgaggcgg agactaatct aacttcggag caggctgaaa aacttaggta
     4081 cggggtggaa ggccgcttgc agatgattac gggcaagaaa agttatctac gttattattt
     4141 ggatcaattt ttgaacaaag agtaatgttc gtgtttttag agttaaataa tttttaaact
     4201 gtgagaaaga ttcttcttgc agttttttct ttacaatttt tgaaaaatat ctactattta
     4261 ttcggttaaa ttcttgtgtt ttttggtttt ttgtggtaaa atgtgctcaa gtaatacgaa
     4321 aggcgaactt taaaatgtca aaacaattga tctattcggg aaaagctaaa gatatctata
     4381 caactgagga tgaaaatctt attatttcaa cttacaagga ccaggcgact gctttcaatg
     4441 gtgtcaagaa ggagcagatt gcaggtaagg gagtcttgaa taatcagatc tcatctttta
     4501 tttttgagaa attaaatgcg gctggtgtgg cgactcactt tgtggagaaa ctttcagaca
     4561 cggaacaact caataaaaag gttaagatta ttcctttgga agtcgtgctc cgcaactata
     4621 ctgctggttc cttttcaaaa cgttttggtg tggatgaagg aatcgccttg gagactccga
     4681 ttgtcgaatt ttactacaaa aatgatgatt tggatgatcc atttatcaat gatgagcatg
     4741 tgaaattcct acagattgcg gatgaccagc agattgccta cttgaaggaa gaaacgcgtc
     4801 gtatcaatga actattgaaa gtctggtttg ctgagattgg gcttaaattg attgacttta
     4861 agctagagtt cggttttgac aaggatggca agattatctt ggcagacgaa ttttcaccag
     4921 ataactgccg cttgtgggat gctgatggca accacatgga taaggatgtt ttccgtagag
     4981 gattgggaga actaaccgac gtttatgaga ttgtttggga aaagttgcag gaattgaaat
     5041 aatctgtttg caatggaaaa ccttcgtctc tcaactaaaa ggactcaggc tgaaaaggtc
     5101 ccccagacct tttcactctg tagagaacta ggtgaactaa ccgatgttta cgaaattgtc
     5161 taggaaaagt tgcagggttt aaaataacaa cctcaaggct gtttgggaat attgcaagag
     5221 ctgaaataaa ggaataagaa ttgatggata aacgtatttt tgttgaaaaa aaggctgatt
     5281 ttcaggtcaa gtcagagagt ttggttagag agctccagca caacttggga ctgtcaagct
     5341 tgaaaagtat tcgtattgtg caagtatatg atgtatttga cttggctgag gacttgtttg
     5401 cacctgcaga gaagcacatt ttctctgagc aggtaaccga ccatgtttta gatgaagtat
     5461 ctgtgcaggc ggatcttgct aactatgctt tctttgccat tgaaagtcta ccagggcagt
     5521 ttgaccagcg tgcagcttcg tcacaggaag ccttgctttt gttgggaagt tcgagtgacg
     5581 tgacagtcaa cacagcccaa ctttacttgg tgaataaaga tattgatgtg actgagttgg
     5641 aagctgtcaa aaactacctg ctcaatccag ttgattctcg tttcaaggat atcacgacag
     5701 ggattgccaa gcaggagttt tcagagtcag acaagaccat tccaaaattg actttctttg
     5761 aaagctatac agcagaagac tttgctcgct acaaggccga acaagggatg gccatggaag
     5821 tggatgattt gctctttatc caagactact ttaagtcaat cgggcgcgtg ccaactgaga
     5881 ctgaactcaa ggttttggac acttactggt ctgaccactg ccgtcatacg acttttgaga
     5941 cagagttgaa acacatcgac ttttcagctt ctaaatttca aaagcaattg cagtcaacct
     6001 atgacaagta tattgccatg cgcgaggaat taggtcggtc tgaaaaacca caaaccttga
     6061 tggatatggc gactattttc ggtcgttatg agcgtgctaa tggacgattg gatgatatgg
     6121 aagtctctga cgaaatcaat gcctgctcag ttgaaattga agtggacgtt gatggtgtca
     6181 aggaaccttg gctcctcatg tttaaaaatg aaacccacaa ccatccaaca gaaattgagc
     6241 catttggtgg agcggctacc tgtatcggtg gagctattcg tgatccgttg tcaggccgtt
     6301 cctatgttta ccaagccatg cgtatttcag gtgctggtga tattacagca ccgatttcgg
     6361 aaactcgtgc tgggaaattg ccacaacaag tcatttctaa aacagcagct catggttatt
     6421 cttcatatgg taaccagatt gggcttgcca caacctacgt tcgtgaatac ttccacccag
     6481 gctttgtagc taaacgtatg gaacttggtg ccgttgttgg tgcggctccc aagggcaatg
     6541 ttgtccgtga aaaacctgaa gcaggtgatg tgatcatcct tctcggaggc aaaacaggtc
     6601 gtgatggtgt cggtggtgcg acgggctctt ctaaggttca aacagttgag tctgtagaga
     6661 ctgctggtgc tgaggttcaa aaaggaaatg ccatcgaaga acgcaagatt cagcgcctct
     6721 tccgtaatgg caatgtcact cgtctgatca agaagtccaa tgactttggg gcaggcggcg
     6781 tctgtgtggc tatcggtgaa ttggcagacg gtcttgaaat cgacctcaac aaggtgcctc
     6841 ttaaatacca gggcttgaat ggtacagaaa ttgccatctc tgaatcacaa gaacggatgg
     6901 cggtcgtggt tcgtcctgaa gatgtggatg ccttcgttgc cgaatgtaac aaagaaaata
     6961 ttgatgctgt tgtggtggcg acagtaactg aaaaaccaaa tcttgtcatg aactggaatg
     7021 gtgagacaat cgttgacttg gagcgtcgtt tccttgacac caatggtgtg cgcgtggttg
     7081 tcgatgccaa agttgtggac aaggatgtca aactcccaga agagcgtcaa acatctgctg
     7141 aaacactgga atcagatacc cttacggttc tatctgacct caaccatgca agtcaaaaag
     7201 gattacagac tgtctttgac tgctctgttg gacgctcaac ggttaatcac ccacttggtg
     7261 gtcgttacca actcacacca actgaggcat ctgtgcagaa attgccagtt caacacggtg
     7321 tgactcatac tgcgtcggtc attgctcaag gtttcaactc atatgtagct gaatggtctc
     7381 cataccacgg tgctgcttat gcggttatcg aagcaactgc tcgtttggtg gctgctggtg
     7441 ccaactggtc taaggctcgt ttctcttacc aagaatattt cgagcggatg gacaagcaag
     7501 cagagcgttt tggtcagcca gtagccgccc ttctaggctc tattgaagct cagattcaac
     7561 ttggcttgcc atctatcggt ggtaaggact ccatgtctgg tacctttgaa gaattgaccg
     7621 ttccgccaac cttggttgcc tttggggtga cgacggcaga tagccgtaag gtgctctctc
     7681 cagaatttaa agctgttggg gaaaatatct actacatccc aggtcaagcc ctctctgcag
     7741 agattgattt tgacttgatt aagaaaaatt ttgctcagtt tgaagcaagc caagctgacc
     7801 ataaagtgac atctgcatca gctgtcaaat acggtggtgt agttgaaagt ttggctcttg
     7861 ctacctttgg aaactatatt ggtgcagagg tgaccttgcc tgaacttaaa acagctttga
     7921 cagctcaatt aggcggcttt gtcttcacat ctcctgaaga aattgctgga gtagagaaga
     7981 ttggacaaac gaaagcagac tttacactga ctgtcaacgg tgtgaagcta gatggacaca
     8041 agcttgacag tgcatttcaa gggacattgg aagaagttta cccaacagaa tttacccaag
     8101 cgaaagaact agaagaagta ccagctgtgg catcagatgt tgtgattaaa gccaaagaaa
     8161 aggttgaaaa acctgtggtt tacatcccag tctttccagg aaccaactca gaatatgatt
     8221 cagctaaggc cttcgaaaaa gaaggtgcag aggtcaattt ggtgccattc gtgaccttga
     8281 atgaagaagc tattgtcaag tcagttgaaa ctatggttga caatatcgac aagactaata
     8341 ttctcttctt tgccggtgga ttctcggctg cggatgaacc agatggttca gctaagttta
     8401 tcgtcaatat cctgcttaat gaaaaagtgc gtgtggctat tgatagcttt atcgcccgtg
     8461 gtggcttgat tatcggtatt tgtaatggat tccaagcctt agtcaaatcg ggtctcctac
     8521 catacggaaa ctttgaagat gctaacagta ctagtccaac cctcttctac aatgatgcca
     8581 accaacacgt ggccaagatg gtggaaactc gcattgccaa taccaactca ccatggttgg
     8641 ctggtgtgca agtgggcgat atccacgcta ttcctgtttc gcacggtgaa gggaagtttg
     8701 tcgtgacggc tgaggaattt gcagagctcc gtgacaatgg acaaattttc agccaatacg
     8761 ttgactttaa cggtaaacca agtatggatt ctaagtacaa tccgaatggt tctgtccatg
     8821 ccatcgaagg aattaccagc aagaatggtc aaatcatcgg taagatgggc cactcagaac
     8881 gttatgagga tggtcttttc caaaatatcc caggcaataa agaccaacac ctgttcgcat
     8941 cagcggttaa acatttcact ggaaaataag acttacagat tttctaatag atagtatcag
     9001 taatgtaaaa gtcatgtaaa tctagctctt gatgattaca aatgaaaatt aggtataaaa
     9061 aatgacatac gaagtaaaat ctcttaatga agaatgtggt gttttcggta tttggggaca
     9121 tccagatgct gctaagttga cctactttgg actccacagt cttcaacacc gtggtcagga
     9181 gggggcagga atcctctcca acgatcaagg acaactgaag cgccatcgtg acatggggct
     9241 tttatcagaa gttttcagaa atccatctaa tttggataaa ttgacaggag ctggtgcgat
     9301 tgggcatgtg cgttatgcga ctgctggcga agcttctgtt gataacatcc aacccttcct
     9361 cttccgtttt cacgatatgc agtttggttt ggctcataat ggaaatctga ccaatgcagc
     9421 ctctctcaag aaagaactgg aacaaagagg agcaattttc agcgcgactt cggactctga
     9481 aatcttggct cacctcattc gtcgcagtca taatcctagc ctgatgggca aaatcaagga
     9541 agcgctcagc cttgtcaaag gtggttttgc ctatatcttg ctgtttgagg acaagttgat
     9601 tgcggctctt gaccctaatg gctttcgtcc gctttctatc gggaaaatgg ccaacggagc
     9661 ggtggttgtt tcctctgaaa cctgtgcttt tgaggtcatt ggtgccgagt ggatttgtga
     9721 tttgaagcca ggtgagattg tgatcattga tgacgagggc attcagtatg acagctatac
     9781 agatgatacc cagttggcga tttgttctat ggagtatatc tactttgccc gccctgattc
     9841 taatatccac ggtgtcaatg tccatacggc acgtaagaga atgggagcgc aattggcgcg
     9901 agaatttaag catgaggcag atattgtagt tggtgtgccc aattcttccc taagcgcggc
     9961 tatgggattt gcggaagaat caggcttacc aaatgaaatg ggtctgatca aaaaccaata
    10021 cacccagcga acttttatcc aaccgactca agaattgcgg gagcaaggag tgcggatgaa
    10081 actgtctgct gtttcgggtg ttgtcaaagg caaacgtgtg gtcatggtgg atgattccat
    10141 tgtacgtgga acaacctctc gtcgtatcgt tcagctcttg aaagaagcgg gtgcgactga
    10201 ggttcacgtt gccattggaa gtcctgcact agcgtatcca tgtttctacg ggattgatat
    10261 ccagacccgt caggagctga ttgcagccaa tcatacggtc gaagaaactc gccaaatcat
    10321 tggtgcggac agtctgactt atctttcaat tgatggcttg attgagtcga ttggtatcga
    10381 aacagatgcg ccgaacggtg gtctctgtgt cgcttacttt gacggtgact acccaacgcc
    10441 tctctatgac tacgaagaag actatcgtag aagtttggaa gaaaagacca gtttttacaa
    10501 gtaggcgaca gattctccat taaagaaaag gaaaaaataa atgacaaata aaaatgcata
    10561 tgcccagtcg ggtgtggatg ttgaagcggg ttatgaagtt gttgaacgga ttaaaaagca
    10621 cgtggcccgt acggagagtg caggtgtcat gggagctctt ggtggctttg gtggtatgtt
    10681 tgatctttcc aagactgggg ttaaagaacc cgtcttgatt tcagggactg acggtgtcgg
    10741 aaccaagctc atgttggcta tcaagtacga caagcacgat accatcgggc aggactgtgt
    10801 ggccatgtgt gtcaacgaca tcattgctgc aggtgcggaa cccctctatt ttctcgacta
    10861 cgtagcgaca gggaagaatg aaccagctaa gctagaacaa gtggttgctg gtgtggcaga
    10921 aggttgtgtg caggctggtg ctgccctcat cggtggggaa acggctgaaa tgccgggcat
    10981 gtacggcgaa gacgactatg acttggctgg ttttgcggtc ggtgtggctg aaaaatctca
    11041 aatcattgac ggttcaaagg tggtagaggg agatgttctt ctcggacttg cttcaagtgg
    11101 gattcactca aatggttact ctttggttcg tcgtgtcttt gcggattaca caggtgagga
    11161 agtcctacca gaattggaag gcaagaaact taaggaagtt ctacttgagc cgactcgtat
    11221 ctatgtcaag gctgtcttgc cgctcatcaa agaagagttg gtcaacggca ttgcccacat
    11281 cacaggtggt ggctttatcg aaaatgtccc tcgtatgttt gcagatgacc tagctgctga
    11341 aattgatgaa agcaaagttc cagtgcttcc aattttcaaa gcccttgaaa aatatggtca
    11401 gattaaacac gaagaaatgt ttgaaatctt caatatgggt gtgggactta tgttggcggt
    11461 cagccctgaa aatgtagagc gtgtaaaaga attgttggat gaagcagtct atgaaattgg
    11521 tcgcatcgtc aagaaagaaa acgaaagtgt cattatcaaa tgaaaaaaat agcggttttt
    11581 gcctctggta atggctcaaa ttttcaggtg attgccgaag aatttccagt ggagtttgtc
    11641 ttttcagacc atcgtgatgc ctatgtgctt gagcgtgcaa agcagctcgg cgttctgtcc
    11701 tatgcttttg aactcaagga gtttgagagc aaggcagact acgaagcagc ccttgtcgaa
    11761 ctcttggaag aacaccagat tgacttggtt tgcctagcag gctacataaa aatcgttgga
    11821 ccaaccttat tgtcggctta tgaaggtcgg attgtcaata ttcatccagc ctacttgcca
    11881 gaatttccag gagctcatgg gattgaggat gcttggaatg ctggcgtggg tcagtctggt
    11941 gtgaccattc actgggtgga ttcgggtgtg gatacaggcc aggtcatcaa acaggttcgt
    12001 gtgccacgac tagctgatga taccattgac agatttgaag ctcgcatcca tgaagcagag
    12061 tacaggttgt atctggaagt agtgaaggct ctatttacag attgactttt tgatgattca
    12121 tatgatatct ttgattttaa attggagtca gtgtttgttg aagacggctt caaacggagg
    12181 tatttgtaat gttagaatct aaaaaaacaa ctcgatatgt attttatgtc tatctgatgt
    12241 tattaacttg gggaatctta tttaagtttg aaacaaatcc tgaatttata gcatttttct
    12301 tagctccaag gtatatcaat tggattccat tttcagaacc actaatagtc gatggaaaaa
    12361 ttgtttttgc tgaaatgtta tttaatctga tttcctttat tccattaggt gtttgtttcc
    12421 ctttgataaa aactaattta tctagtttaa gaatagtcgg gacaggtttc ttgattagtt
    12481 tattgtttga gtgcttacag tatattttag caataggtat aacagatata acggatttga
    12541 ctttaaatac gctaggtgtc tgtgtaggct tactgattta tcaaattttt ataagagtgt
    12601 tcaaatcaca gactagaaaa tggatcaata tcttaggtat gcttagcctt ggttttgctt
    12661 atcttgtttt actgttactg catttaattg gtgtttaact aatgattaaa aaggagaata
    12721 taatgactaa acgcgtctta atcagcgtct cagacaaagc gggcattgtt gaatttgccc
    12781 aagaactcaa aaaacttggt tgggagatta tctcaacagg tggaactaag gttgcccttg
    12841 ataatgctgg ggtggatacc attgctatcg atgatgtgac tggtttccca gaaatgatgg
    12901 acggtcgtgt gaagaccctc cacccaaata tccacggagg gcttctcgct cgtcgtgact
    12961 tggatagcca cttggaagcg gctaaggaca acaagattga gctcattgac cttgtggtgg
    13021 tcaaccttta cccatttaag gaaactatcc ttaaaccaga tgtgacttat gctgatgcag
    13081 ttgaaaatat cgatattggt gggccatcta tgcttcgttc agcagcgaaa aatcatgcca
    13141 gtgttacagt tgtggtagat cctgctgact acgctgtggt tttggacgaa ttggcagcaa
    13201 acggcgaaac ctcttatgaa actcgccaac gtttagcagc caaagtattt cgtcacacag
    13261 cggcttatga cgccttgatt gcagaatact tcacagctca agtgggtgaa agcaagcctg
    13321 aaaaactcac tttgacttat gacctcaagc aaccaatgcg ttacggtgag aatcctcaac
    13381 aagacgcgga cttttaccag aaagctttgc ctacagatta ctccattgct tcagccaaac
    13441 agctcaacgg gaaagaattg tcatttaata atatccgtga tgcagatgct gctatccgta
    13501 tcatccgtga cttcaaagat agtccaaccg ttgtggctct caaacacatg aatccatgtg
    13561 gaattggtca agctgatgac atcgagactg cttgggaata cgcttatgag tctgacccag
    13621 tgtctatctt tggtggaatt gtcgtcctca accgtgaggt ggatgctgcg acagctgaga
    13681 agatgcacgg cgttttcctc gaaatcatca ttgcaccaag ctatacggat gaagcgctag
    13741 ccattttgat caataaaaag aaaaacttgc gtatccttgc cttgccattt aatgctcaag
    13801 aggctagcga agtggaagca gaatacacag gtgtagtcgg tggacttctc gtgcaaaatc
    13861 aagacgtggt caaggaaagc ccagctgact ggcaagtggt gaccaaacgt cagccaaccg
    13921 agacagaagc gactgctctt gagttcgctt ggaaggctat caagtacgtc aaatcaaatg
    13981 gcattatcgt gaccaacgac cacatgacac ttggtgttgg tccaggtcaa accaaccgtg
    14041 tggcttctgt tcgccttgcc attgaccaag ccaaagatcg tctggacggg gcggtccttg
    14101 cttcagatgc cttcttccca tttgcggata acgtggaaga aatcgccaaa gcaggaatta
    14161 aggccatcat ccagcccggt ggctcagtcc gtgaccaaga atctatcgaa gccgctgaca
    14221 agtatggctt gaccatggtc ttcacaggag tgagacattt tagacattaa gaagataaaa
    14281 gggaagaaaa cagtttcttt ccttttttgg cttaaaatgc taactgaaac aagattaaaa
    14341 cgaacttttt tgatataatg ttggtaaata attcgcaaaa gaggttgagg aatgaaactg
    14401 cttgttgtcg gttctggtgg tcgtgagcat gcgattgcta aaaagttact tgaatcaaaa
    14461 gacgtggaaa aagtctttgt agctcctggg aatgatggga tgactctgga tggtttggaa
    14521 ttggtaaata tctctatttc cgaacattct aaattgattg acttcgcaaa gaccaatgat
    14581 gttgcttgga cctttatcgg cccagatgat gcccttgctg ctggtatcgt ggatgatttt
    14641 aaccaagctg ggcttaaggc ctttggtccg actagggctg cagcggagct ggagtggtcc
    14701 aaggattttg ccaaggaaat catggtcaaa tatggtgttt cgacagcagc ctatggcaca
    14761 ttttcagatt ttgaggaagc aaaggcctat atcgaaaagc atggtgcgcc tatcgtagtc
    14821 aaggcggatg gcttggcgct tgggaagggt gtcgtcgttg ctgagacggt tgagcaagcg
    14881 gtcgaagccg ctcatgagat gcttttggac aataaatttg gtgactcagg tgcgcgcgtg
    14941 gttattgagg aattccttga aggagaggaa ttttcactct ttgcctttgt caatggtgat
    15001 aagttctaca tcatgccaac ggctcaggac cacaaacgtg cctacgatgg cgacaaaggg
    15061 cctaacacgg gtggtatggg tgcctatgcg ccagttccac acttaccaca gagtgtagtt
    15121 gatacagcgg ttgacaccat tgtcaagcca gttctagaag ggatgattaa agaaggtcgc
    15181 ccttatctgg gagttcttta cgcagggctt atcctgacag ctgatggacc gaaagtcatc
    15241 gagttcaacg ctcggttcgg agatccagaa actcagatta tcttgcctcg cttgacctct
    15301 gactttgctc aaaatatcac agatatcctg gatagcaagg agccaaatat cacgtggacg
    15361 gacaagggtg tgactctggg tgtggttgtc gcatccaagg gctacccgct agattattca
    15421 aagggtgttg agttgccagt caaaaccgat ggtgacatca ttacctacta tgcaggggct
    15481 aagtttgcgg aaaatagcag agcgctgctc tcaaacggcg gacgagttta tatgctcgtt
    15541 accacagcag ataccgtcaa agaagcccaa gccagcatct accaagaact atcccaacaa
    15601 aaaatagaag gactcttcta ccgaacagat atcggaagca aggcaattaa gtaaagatat
    15661 aagaataacg cgacgcagtc gccaaacacg ataatggtcg tcgtggtgaa aagaccagaa
    15721 cagtgaatgt tctggtcagg gggaaacttt gagaccttag gctcaaagtt taggaatgaa
    15781 accgaaggtt tgcttccgcc tccatcacct aagaccatta tcaaaaagaa aaataaaaat
    15841 tcacaaaata cgttaatggt cgtatggttt gcgagcgtta gcgagctaat atggagcaat
    15901 taccgccgtt gtgaaagaac gattggatga taatccaatc gttcagggaa attggaagac
    15961 cttgggtttc caatttaggc atgagacacc tttggtggct gctgccgtcc ctcacaagct
    16021 aaggtgattg ttgaaaaaga ggaaaaagga gaagaaatga aaccagtaat ttccatcatc
    16081 atgggctcaa aatccgactg ggcaaccatg caaaaaacag cagaagtcct agaccgcttc
    16141 ggtgtagcct acgaaaagaa agttgtttcc gcacaccgta caccagacct catgttcaaa
    16201 catgcagaag aagcccgtag ccgtggcatc aagatcatca tcgcaggtgc tggtggcgca
    16261 gcccatttgc caggtatggt cgctgctaaa acaatccttc cagtcattgg tgtgccagtc
    16321 aactctcgtg ctcttagtgg agtggattca ctctattcta tcgttcagat gccgggtggg
    16381 gtgcctgttg cgaccatggc tatcggtgaa gcaggtgcga caaatgcggc cctcttcgct
    16441 ctccgtctcc tctcagtaga ggatcagtct atcgcgacag cattggcaga tttcgcagaa
    16501 gaacaaggaa aaatcgcaga ggagtcgtca aatgagctca tctaaaacaa tcggaattat
    16561 cggtggcggt caactgggtc agatgatggc catttctgct atctacatgg ggcacaaggt
    16621 tatcgcgctg gatcctgcgg cggattgccc ggcctctcgc gtggcggaaa tcattgtggc
    16681 accttataac gatgtggacg ccctccgtca gttggcagac cgttgcgatg tcctcactta
    16741 cgagtttgaa aatgtcgacg ctgacggttt ggatgtcgtt atcaaggatg gacaactccc
    16801 tcaaggaaca gatctgctcc gcatttcgca aaatcgtatt tttgaaaagg actttttgtc
    16861 aaacaaggct caagtcactg tggcacccta caaggtcgtg acttctagcc tagacttggc
    16921 agatatcgac ttgtcgaaaa actatgtcct taagatcgca acaggtggtt atgatggaca
    16981 cgggcaaaat gtcattcgtt cagcagacga tttggaagaa gcctatgcat tagccgactc
    17041 agcagcttgt gtcttggaag aatttgtcaa ttttgacctt gagatttctg tcatcgtgtc
    17101 aggaaatggg aaggaggtga cgtttttccc agttcaggaa aatatccacc gcaacaatat
    17161 cctgtctaag accatcgtac cagcccgtat ttctgaaagt ctagcagaca aggctaaagc
    17221 tatggcagtg cgaatcgcag aacaactcaa cttgtctgga actctctgtg tggaaatgtt
    17281 tgcgacagct gatgacatca ttgtcaatga gattgctcca cgaccacaca actcaggcca
    17341 ctactctatt gaagcctgtg acttttcaca atttgacacg catatattag gtgttctggg
    17401 agcaccattg ccagccatca aactacatgc gcctgccgtc atgcttaacg ttctcggcca
    17461 gcatgttgag gccgctgaaa aatatgtcac agaaaatcca agcacccacc tccacatgta
    17521 tggtaaaata gaagcaaagc ataatcgtaa gatgggacat gtgactttgt ttagtgatgt
    17581 accggatagt gtggaagagt ttggggaagg gattgatttt taggacaagt ctatgataca
    17641 aattatcgtt aatgcattta ttgaaaagga taagactgga gcagttgttg aagtgttgta
    17701 tgctagtgct gaccaagata aggtacaagc taaatatgaa gaactagctg cacaataccc
    17761 cgaaaattat ttagctatct atgatgtacc gctggatacg gatttgaata cactagatca
    17821 ttacccgtct gtgtttattg gaaaagagga gtttgagtag aaatcttggt ttacctagat
    17881 agcttattcc caacagctta agaagaaagg aaagattaac acatgatcaa ccgttactct
    17941 cgccctgaga tggcgaatat ttggagtgaa gaaaataaat accgtgcttg gcttgaggtg
    18001 gaaatcctct ctgacgaggc atgggctgag ttgggggaaa tccctaagga agatgtggct
    18061 ttgattcgca agaaggcgga ctttgacatc gaccgtattt tggaaattga gcaggagacg
    18121 cgccacgatg tggtggcttt cacgcgtgcg gtttctgaga ctcttggtga agagcgcaag
    18181 tgggttcact atgggttaac ttctaccgac gtggtggata ctgcctatgg ttacctctac
    18241 aagcaggcca acgacatcat ccgtcgtgac cttgaaaact tcactaatat catcgctgac
    18301 aaggccaagg agcacaagtt caccatcatg atggggcgta ctcatggtgt gcacgctgag
    18361 ccgacaactt ttggtcttaa attggcaact tggtatagcg aaatgaaacg caatatcgag
    18421 cgcttcgagc atgcggctgc tggtgtagaa gctggtaaga tttctggtgc ggttgggaac
    18481 tttgccaata tcccaccatt tgtagaggag tatgtctgcg ataaacttgg catccgtgcc
    18541 caagaaatct ctacacaagt ccttcctcgt gaccttcacg ctgagtactt tgcggttctt
    18601 gccagcattg cgacttcaat cgaacgtatg gcgactgaga ttcgtggtct acaaaaatct
    18661 gagcaacgcg aagtagaaga gttctttgct aaagggcaaa aagggtcttc agcaatgcct
    18721 cacaaacgca acccaatcgg ttctgaaaat atgactggtc tggcgcgtgt cattcgtggt
    18781 cacatgatta cggcttatga aaacgtcgct ctctggcacg aacgcgatat ttctcactca
    18841 tcagctgagc gtatcatcac accagatacg accattttga ttgactacat gctcaaccgt
    18901 tttggaaata tcgtcaagaa cttgacagtc ttcccagaaa atatgatccg aaacatgaac
    18961 tcgacttttg gtcttatctt tagccaacgg gctatgttga cattgattga aaaaggcatg
    19021 acccgtgagc aagcctatga cttggtgcaa ccaaaaacag cctactcttg ggacaaccaa
    19081 gtagacttta aaccacttct tgaggcagat tcagaagtaa catcacgtct cactcaagaa
    19141 gaaatcgatg aaatcttcaa cccagtttat tacaccaaac gagtggatga tatctttgaa
    19201 cgtcttggac taggtgatta attaaaaaat aaacagcgag cttcaatctc gctgtttatt
    19261 ttttatcgaa aagacttagt cttcttttct tttagtgagt ccataggctg ctagtgtgga
    19321 catgagtcct gcgactacta gtcctgcaga atcgtgagtt cctgtttcag gaagtttttt
    19381 ctctgttacc acaggagctg gatcttgagg aagagctttg ctttcctcag caggagcagt
    19441 tgatggagct ggttggcttg ggatttctag ttttggtttt tcttcagcaa tagcggcttg
    19501 tccgttttca tcgcctacat gtgttaccat agttccgact tcgactattt gagtaacggc
    19561 ttcctgtgct acgacactat ttacaagtgt tttcacttcc ttaccatcgg cagaagtgct
    19621 cacagagtag aagttgctac gatgtccatt gacgccctta gtaatgactt gtgtttttcc
    19681 tttgagtaag agtggatttt cacgagtcac tgtggtaaat ggaatttctt ctttttggat
    19741 atccagtcta ggttttacct cagtagttgg tgcaagacca ctttcatcac ccttgtgagt
    19801 tacaggagcg ccaacttcaa ccacttggtt tataacttct ttggttacct ggctatcaag
    19861 gactgtttct gttgtttttc cattttcagt gagtacagag atgtaatgag ttcgttcacc
    19921 tttgactcct gctgtgataa tattttcctg accagctggg aggttaggat tttctttctt
    19981 gataacttca aatggaattt cttcagttct tgtgatgagt tctggtctgg tttcaacatt
    20041 ggcagccact tcattttcat ctaggcttcc tgaatgagtt gcagctggtt tgaggccttg
    20101 aagagcggct tttaggttgg ctacaagtgt gtcaagctca gcttgtttat tacggttgag
    20161 gttgtaattt agagctgttt tagctgcgtc aagggcctca agactttctt tactatatcc
    20221 ttctaagttt gtaggaattt tagctaattc ttcgcggaga gcattataat tagcacggaa
    20281 gtagtctttg ttgtggtctg caaaggcagt catgagttca aagatttcct cttccttgta
    20341 ctcagcgctt ggtctatctg cccagattga aagcatactt ccgactgttg gaagatctac
    20401 ttcaggatat ttggtagaag ctagttgatt gaatggtgtt tttccagtat tctcaatagc
    20461 tttcttgagg aaaccaccac catcttctgg tttttgacca agaatgtagt accagtcacc
    20521 gttggtattc aagaatttat agcctttgct tgctaggtat tgaggtgatg cgaggttata
    20581 tccccaccag cctttagacc agtaagaaat caagacatct ttgtcaaact gaacatcgtc
    20641 cttgtcttca tagtagaagc catcgttgaa ggccattggt tgaagccctc tttctttggc
    20701 catagctgcg agggtgttgg catattcggc aaatttgcca tagagttgat accacttgag
    20761 gtagtaccag ccttgggcac tagtcgcatc gttggcgtat tcgtcagtac caaagttgaa
    20821 aatctttgtt ttacctgcaa agaagtccat gtatttaccg atgagggctt ttacaaagtt
    20881 catcgcttct tcgtttttca agtccatagt tgtttttgaa actttatcaa agtgggcttg
    20941 aggattttta atacctaatt tttccatggc aaccagcata gcatccatgt gacctggact
    21001 gttaatagct gggatgagac cgatgtcctt agatttagcg tattcaatta gctctgttac
    21061 ttctgcctgt gttagtgcag taccgtttgg atcgtcgtag taagctttag ttccttcgat
    21121 aatagctttt ttaacgtcat cactagcata ggtttttccg ttggcagtaa tggtcatatc
    21181 atcgagtaga aagcgaagtc cgtcatttcc tagaaggaga tggacatcag aatatccgag
    21241 ctcactggcc ttgtctacga tgcgtttgag ctggttcaga gtaaagtatt tgcgtccagc
    21301 atcgattgag attaccttgt ttttggcaag tttttcaacc tcacgtttag cttcttcttc
    21361 ttttagagct tcaggcgtga gggtcaagtt gttgacagtt tcttgaagtt tagcaatggc
    21421 ttgatcaatc gtatcttgtt gggcacggct aaggttgcta tcgagagagc gaatagcttt
    21481 ttcagcttct tttacggccg tgacgctttc tgcagtataa cggttcaggt cttttggtac
    21541 ctcgttaagt gcttgctctg cagattcata atcagctgcg aagtattcag cgttggcatt
    21601 tgcaaaatga cgcatgagtt tgaagaggcg tgatggtgaa taacgtgcag atggagtgtc
    21661 agcccaagca gctaccatac caccgatgat tgggatatca gctccttctg tttttggtac
    21721 agaagtgatt ggtgtgtttt taataccatt gagcccctga tcgagattgt accagccttg
    21781 gccatcagcg tttcgtccaa gaacgtagta ccaagcatca ttggtattaa ggatttggtg
    21841 acctttttca gctagtagtt tagaagaagc gacatcgtag cctccccaac caccagtcca
    21901 catagaaacg atgatgtctt tgtcaaaact accaaagctt gtgtcgctat tgtagtagat
    21961 accgtcgtta aaagccattg gtttgagacc gtgcgatttt acaatacgag cgaggtcatt
    22021 ggcgtaggca ataaattttt catagccttt tacagggtag ccttcgtttg gatagtattt
    22081 atcagcttga agcacactcc aacctttagc atctgtcgca tcattggcat attcatcaag
    22141 tccgatgttg aagatttcag tctttttcgc gaaataagca gcatacttgt cgataagggc
    22201 ttttgtaaaa gcgacagctt gttcgttgtc aagatcgaca gtacgggctg atttcttccc
    22261 aaaatagcta aagttagggt tttggattcc caattctttc atggcattga gaatcgcatc
    22321 catgtgtcca ggactattta ctgtcggaat gagaccgata cctttatctt tggcatagtt
    22381 aatcagatct gtcatttgac tttctgttaa gtgattgccg tttggatcgt tgtaataatc
    22441 atttgtacct ttttcaatgg cgcgtttgac atcgtcactg gcataggtct tgccgttagc
    22501 tgtgatgctc atatcgtcca acatgaaacg gagtccatca tttccgacta ataggtgtaa
    22561 atcagtgtag ccataatgtt tcgctttatc gatgatttcc ttgagttgtt ctggtgagaa
    22621 atatttacgt ccagcatcaa tagaaacaat tttctttttc gctagttttt catttacagt
    22681 tgcagcacgt tcctttcctg cctctgtttc cggtttgtca gcctctgctt tcgcttcatc
    22741 ttttttagct ggtttatcct tgtcagtctt gtctgtattt gactctttag aatcaacctc
    22801 tttcgcttct tcctttttag ggctagcttc ttctgccttt ttattagcag tttctttttc
    22861 agcagaagtt ggagttacca cctctgcttt atcactagga gttgaactaa cttcctcttg
    22921 tggtttttct tctgtttttg gaagactagc taccttatca gtagctggag tttctgtttc
    22981 tacagttttt ggagcttctg gttgaagctc tgctttaggt gtttcctcag tccgattttc
    23041 ggatgattga ggggaatcag aaactgtatg gatggtcggt tggttttctg tagtagtagt
    23101 aactccatcg gctgcaacag tctgtgcttg gaaggcaaat ccaattagaa cagaagctgc
    23161 tcctacagcg tatttacgaa tagaaaaacg ctgttgtttt tcatgtttca ttgcaaaacc
    23221 tcctgattgc attgttatat tgatagcgat tatataaatc aacgccttta ttttatttct
    23281 tatattaacg agagtcaaga ggagatgaca aaaaactata ataagtataa aaaaatataa
    23341 aatttaagct taagatttca gattggtcgg aaaaaatacg tatatatatc tagtataatt
    23401 tttggttcta tttctataaa atattccaca aattatagaa tcttccaaaa ataggtaagc
    23461 gctacctttt tggtgtggta taataagcat agaaaaagcc cgagcgatta ggctcaggct
    23521 ttcttcttag tgatcacggt cacatgagat gaatttaatc ttgtagtaat cagatcgttt
    23581 gtaagtttca ctgtattcta aaacttggcc agttgattcg agtttggtga ttttagtttg
    23641 taggacagta gggaattgtt catcgactcc gaggactgaa gccgcatgtt ctggagttgg
    23701 aaagactatt tcgttgattt cttcaaagtg ttcatcattc atgtgaatgt ggtagtctaa
    23761 cttgaaacga ttatagatag aactatagta ttcaaggttt ggataatttg cgttgatata
    23821 ttgttctggg atgtaggatg tatggtagat ataaacgaca ccgtttgatt cgcggatacg
    23881 ttcaatcttg tagtagaatt gatcgccgcg tagacccaat ttttccaagt aaacaagctt
    23941 gtttccgcgt tcaattgaaa gaacagttac cttatcatct ttagcattga agagttcaat
    24001 atctgaaaac tctacaagct tgtgtttgcg tgcacgtgaa acgaaggttc cttttccttg
    24061 ttggcggaca atatagccat ctttggcaag gtcgtttaag gcgcgaacaa ctgtgataga
    24121 gctgacatcg tacattgaaa tgagttctgc ttcagtataa aatttatctc cactgctaaa
    24181 ctgcccagag atgattttat tttttaattc gtcttttatg tattgatatt tgggaattgc
    24241 catattttta cctctatttt ccttctccat agaaaatttt accataaaag cgaaaaaaat
    24301 agtagtcttt tgaataaaaa attagaataa tagtttgaaa aattaagcta tatagggatt
    24361 gatcatattt tataaatgta ttctatacaa aattatttta ggtcctatat tccaaattta
    24421 aagagcgttt gaaatgggtc taaaaatgtt tgaaactaat tgaatgtgtg tataatagta
    24481 aaaatgcagt aaatatcccg acaatttcta ttgacagttc aaacagattg gtttataatt
    24541 aatataacaa caaatgaaag cgcaaacttt cgcggtcgga aggtagtttt atgacacgat
    24601 ttgagatacg agatgatttc tatctcgatg gaaaatcatt taagatttta tctggtgcca
    24661 ttcattattt tagggttcct ccagaggatt ggtatcattc gctctataac ttgaaggctc
    24721 ttggttttaa tacggtagag acttatgttg cttggaattt acacgagcct cgtgaaggtg
    24781 agtttcattt tgaaggtgat ctggatttag agaaatttct ccaaatagcg caggatttgg
    24841 gtctctacgc aattgtgcgt ccgtctccat ttatctgtgc ggaatgggaa ttcggtggct
    24901 taccagcttg gctcttgacc aagaacatgc gaattcgctc atctgaccca gcatatatcg
    24961 aggcagttgg tcgctactat gatcagttat tgccaagact ggtgcctcgt ttgttggaca
    25021 atggtggcaa tattctcatg atgcaggttg aaaatgagta tggttcttac ggagaagata
    25081 aggcttacct gagagcgatt cgacagctaa tggaagagtg tggcgtaacc tgtcccctct
    25141 ttacatcaga tggtccatgg cgagctactc tgaaagctgg aaccttaatt gaagaggacc
    25201 tctttgtaac aggaaacttt ggttctaagg caccttacaa cttttcgcag atgcaggaat
    25261 tctttgatga acatggtaag aaatggccac tcatgtgtat ggagttctgg gatggttggt
    25321 tcaatcgctg gaaagaaccg attatcacac gggatcctaa ggaattggca gatgcagttc
    25381 gagaggtttt ggaacaaggc tctatcaatc tttacatgtt ccacggtggt acaaactttg
    25441 gtttcatgaa tggttgctca gctcgaggaa ctttggacct gccacaagtt acgtcttatg
    25501 attacgatgc ccttctggat gaagaaggaa atccaactgc taaatatctt gcagtcaaga
    25561 agatgatggc aacacatttt tcagagtatc cgcagttgga accactctac aaagagagta
    25621 tggagttgga tgctattcca ctagttgaaa aagtttcttt gtttgaaacc ttagatagct
    25681 tgtcaagtcc tgtagaaagt ctctatcctc aaaagatgga ggagctggga caaagttatg
    25741 gctacctact ttatcgaaca gaaacaaact gggatgcaga agaagaaaga cttcgtatca
    25801 ttgatggtcg agatagggcc cagctgtatg tcgatggtca gtgggttaaa actcaatatc
    25861 agacagagat tggggaagat attttttatc aaggtaaaaa gaaagggcta tctaggttag
    25921 atatcttgat agaaaatatg gggcgtgtca actatgggca taagttctta gcggatacgc
    25981 aacgtaaggg aattcggaca ggggtctgta aggatctgca tttcttacta aactggaaac
    26041 actatccact cccactagac aatcctgaga aaattgattt ttcaaaagga tggactcaag
    26101 gacaaccagc cttttacgct tatgacttta cagtcgaaga gccaaaagat acttacctag
    26161 acttgtctga gtttggtaag ggggttgcct ttgtcaatgg gcagaatcta ggacgttttt
    26221 ggaacgttgg cccaactctc tcactttata tccctcatag ctatctcaag gaaggtgcca
    26281 accgcatcat tatctttgaa acagaaggtc aatataaaga agagattcat ttaactcgta
    26341 aacctacact aaaacatata aagggggaaa acttatgaca attgtaggat gccgtattga
    26401 tggacgtttg atccacggac aagtagccaa tctttgggct ggaaaactaa atgtttcacg
    26461 cattatggtt gtagacgacg aagttgtcaa caacgatatt gaaaagagtg gtttgaaact
    26521 tgcgacacca ccaggtgtga aattgagtat tttgccagtt gagaaagctg cagccaatat
    26581 tcttgctggc aaatacgata gccaacgtct ctttatcgtg gctcgtaaac cagaccgctt
    26641 ccttggtttg gtagaagcag gtgtaccact tgaaaccctt aatgttggga atatgtctca
    26701 aacaccagaa actcgttcta ttacacgttc tatcaacgta gtagacaagg atgtggaaga
    26761 cttccacaaa ctggcagaaa aaggtgttaa acttactgct cagatggttc caaatgatcc
    26821 aatttcagac tttttgagct tattaaaata ggaaaaaaat ttttaggagg tcattgttat
    26881 gatacaatgg tggcaaattt tacttctcac tttgtactca gcttatcaaa tctgtgatga
    26941 gttgacgatc gtttcatctg caggttcccc tgtatttgct ggtttcatta ctggtttaat
    27001 catgggagat gtgactactg gtttacttat cggtggtaac ttgcaactgt tcgttcttgg
    27061 ggttggtacc ttcggtggtg cttctcgtat cgacgcaact tctggtgcgg ttcttgcgac
    27121 agccttctct gtttcacaag gaattgatgc accgcttgcc attactacaa tcgctgtacc
    27181 agtagcagct ctcttgactt acttcgacgt tcttggtcgt atgactacta ccttcttcgc
    27241 tcaccgtgtg gatgctgcaa tcgaacgctt tgactataaa ggtattgaac gcaactactt
    27301 gcttggtgcg attccgtggg ctctatctcg tgcccttcca gtcttctttg cccttgcttt
    27361 tggtggtgcc tttgtacaat cagtagtaga cttcgttgaa gcctacaaat gggttgcaga
    27421 tggcttgaca cttgcaggac gtatgcttcc aggtcttgga tttgcaatct tgcttcgtta
    27481 ccttccagtt aaacgtaacc ttcactacct tgctatggga tttggtttga cagctatgtt
    27541 gactgttctt tactcatatg taacaggtct tggtggcgct gttgctggta tcgtaggtac
    27601 tcttcctgct gaagttgctg aaaaaattgg tttcgtgaac aacttcaaag gtttgtctat
    27661 gattggtatt tctatcgtag gtattttcct tgcagtgctt cacttcaaaa atagccaaaa
    27721 agtagctgta gcagcacctt ctacaccatc agaaagtggg gaaatcgaag atgacgaatt
    27781 ctaattacaa acttacaaaa gaagatttta atcaaatcaa caaacgtagc ttgtttactt
    27841 tccaattagg ttggaactac gaacgtatgc aagcttctgg ttacctttac atgatcttgc
    27901 ctcagttgcg taaaatgtat ggtgatggaa ctcctgaatt gaaagaaatg atgaaagttc
    27961 atactcaatt cttcaatact tcaccattct tccataccat tatcgctggt tttgaccttg
    28021 ccatggaaga aaaagatggt gtaggttcaa aagacgccgt taacggtatc aagacaggtt
    28081 tgatgggacc attcgctcct cttggggata caatctttgg ttcacttgta cctgctatca
    28141 tggggtcagt cgcagcaact atggctatcg ctggccaacc ttgggggatc ttcctttgga
    28201 ttgcagttgc agtagcgtat gacatcttcc gttggaaaca gttggaattt gcttacaaag
    28261 aaggggttaa ccttatcaac aacatgcaaa gtaccttgac agctttgatt gacgctgcat
    28321 ctgtacttgg tgtcttcatg atgggtgctc ttgtagcaac agtgattaac tttgaaattt
    28381 cttacaagtt gccaatcggt gaaaagatga ttgatttcca agacatcttg aaccaaatct
    28441 tcccacgttt gcttccagca atctttactg cctttatctt ctggttgctt ggtaagaaag
    28501 gtatgaactc tactaaagct atcggtatta ttatcgtact tgctttggct ctttctgccc
    28561 ttggtcactt tgcacttgga atgtaattcc ttatgactaa atcattaatt ttggtgagcc
    28621 atggtcgctt ctgtgaggag cttagaggta gcacagaaat gattatgggc ccacaagaca
    28681 acatttacac agtagctctt cttccagaag atggcccaga agaatttact gctaaatttg
    28741 aagctgttat tgaaggattg gatgatttcc tagtctttgc ggatcttctc ggtgggacac
    28801 cttgtaatgt ggtgagtcgc ttgatcatgg aaggtcgtga tattgacctt tacgcaggga
    28861 tgaatcttcc aatggtgatt gaatttatca atgcgagcct tacaggcgca gatgcggact
    28921 acaagagccg tgctgcagaa agcattgtga aagttaatga cctgttagcg ggcttcgatg
    28981 atgacgaaga tgaataatac tcttcgaaaa tctcttcaaa ctacgtcaac gtcgccttgc
    29041 cgtaggtata tgttactgac ttcgtcagtc ttatccggca acctcaaaac ggtgttttga
    29101 gctgacttcg tcagtcttat ccggcaacct caaagcagtg ctttgagcag cctgcggcta
    29161 gtttcctaca gattttagtt ggaactcgat tcaattcatg tgacaacgtg aaaatcgtta
    29221 gagcatttta tatagaatat acatgggaat gtagcttact cccattccca tatttaatag
    29281 aaaaagagga actcaatgct acattataca aaagaagact tgctcgaatt gggtgcagaa
    29341 atcactacgc gtgaaatcta ccaacagcct gatgtatgga gagaagcttt tgaattttat
    29401 caagcaaaac gtgaagaaat tgcagccttc ctacaagaaa tcgctgataa acatgactat
    29461 attaaggtta tcttgacagg tgctgggact tctgcttatg tgggagatac cttgctacct
    29521 tattttaagg aagtctatga cgaacgcaaa tggaatttca atgctattgc gacaacagat
    29581 atcgttgcca atccagcaac ctatttgaaa aaagatgtgg caactgtcct tgtgtctttt
    29641 gctcgtagtg ggaactcgcc tgaaagtgtg gcgactgttg atttggccaa atccttggtg
    29701 gatgagcttt atcaagtgac gattacttgt gcagcagatg gtaaattggc tcttcaagct
    29761 cacggtgatg accgtaatct cttgctcttg caaccagctg tctctaatga tgctggattt
    29821 gccatgactt ctagctttac gtctatgatg ttgacagctc tcttggtctt tgatcctaca
    29881 gaatttgctg ttaagtctga acgttttgaa gttgtatcta gtcttgcccg taaagtttta
    29941 gacaaggcag aagatgtcaa agagcttgtt gatttagact ttaaccgtgt catctatcta
    30001 ggcgctggtc ctttctttgg acttgctcat gaagctcagc tcaagatttt ggaattaact
    30061 gctggtcaag ttgcgaccat gtatgaaagc ccagttggct tccgtcacgg tccaaaatct
    30121 cttatcaacg acaatacagt tgttttggtc tttggtacaa cgacagacta cactcgtaag
    30181 tatgacttgg acttggttcg tgaagttgct ggtgaccaga ttgctcgtcg tgttgtgctt
    30241 ttgagtgatc aagcttttgg tcttgaaaat gtcaaagaag tggcccttgg ttgtggcggt
    30301 gtcttgaatg atatttaccg tgtcttccct tacatcgttt atgcccaact ctttgcccta
    30361 ttgacttcac tcaaggtaga aaataaacca gatacaccgt ctcctacagg tacagtaaac
    30421 cgtgtagtac aaggtgtcat aattcacgaa tatcaaaagt aagacagtgt ttatgaattc
    30481 ttgacaagag gatttgtaaa ttatcagata aaccatagat tgtcagtacg ctttctatgg
    30541 tttgtttgct tgagagaaat agtaaaagga gaacagaatg aaagcataca cagagcgtgt
    30601 atttggaaat gttgagggcg aggatgtctt ggcctatcga tttgagacag acggtggcta
    30661 ccaacttgag gttatgactt atggcgcgac catcttgcgc tatgtcgcac ctgacaaggc
    30721 tggaaatttt gccaatgtta tcttgggatt tgatgacttt gatagttatg taggcaatag
    30781 tcccaagcat ggagcaagtg taggtcctgt agcgggtcgt attgcaggtg cgacctttga
    30841 gctcaatggt aagacctatg accttgaggt taataatgct agcaactgta atcacagtgg
    30901 ttcaactggt tgggattcca gcttgtttga agttgaagaa gtaagcgatc atggcttgac
    30961 tctctacaca gagcgtacag atgggacagg agggttccct ggaaatctca agatttggat
    31021 cagttatcac ttggaagaaa ctggtgccta tgaaatcagc tacaaggtaa cgaccgatca
    31081 ggatacgctg gtcaatccaa ccaaccacag ctatttcaac ttgtctggtg atttcacgca
    31141 gacgattgac cgtcatgtct tccaactaaa cacagagggc atttactcaa tcgctcctga
    31201 cggtgttcct gccaaaactc cagaagccaa ccgtgatgtg gtcaaacaca tctacaatgg
    31261 tgccttgttg aaggatatct ttgcagaaga agatgagcaa atccagctgg catcaggttt
    31321 ggatcatcca tttgcccttc ctgcaggcca tgacaatgct ggattccttt atgaccaaaa
    31381 ttcaggtcgc ttcctgcttt tcaagacaga agctccttgc tttgtggtct acacagcaaa
    31441 ctttgtggat gaaagtgtca tcataggagg tcagccaatg ctacagcaca atgggattgc
    31501 tcttgaagcg caagctttac cagatgccat tcacagtgac cttaaaggcc aagtcattct
    31561 taaagctggt caaaccttca ccagtaagac acgttatgaa cttgttgtga aataaaaaaa
    31621 gctggtcaaa aagtctttaa aattaaaata atgcatattg tcaggtgatg aaaagttcga
    31681 taatatgcgt tttattatga aatgatttac tttatattat cctgaaatgg tgttattttt
    31741 gagttatctg gcttgacttt cttgatggaa attatataat agttgtaaca ataataatta
    31801 caactagaaa agaggaccat ttatgaccta tgaatacaag agtcacattt atttggcaga
    31861 gacagtttta aatataaagg atttggccag tcaaacagcc ttttatcagc aagttattgg
    31921 tttagaaatc ttatctcaaa ctgagacaga gtccattcta ggccttggtg gaaaagtctt
    31981 ggtacagctg attcaagcac aagagagcgg agaagtgagg gaacattatg gtctttacca
    32041 tttggccatt ctcttgccca cacgcaaggc tttggctgat gtcttgaaac acctgacaga
    32101 tttacagatt cctcttgtcg gcggtgcaga ccatggttac agtgaagcta tttacttgga
    32161 agatttggag ggaaatggca ttgaactcta tcgagataag ccagtttcca catgggatat
    32221 tcgagaagat ggacgcatta tcggagtgac ggaggctctt gcggcgcagg acatttatga
    32281 gttgggggaa agagtagagc cctttatcct ggcagaaggt acgagaatgg ggcatattca
    32341 tctttccgtc aaggatagtc gaaagtccag ccagttttat caaaaggtgt tagggctaga
    32401 ggataaattc agtgtgccta gcgctagttg gattgcggct ggagattacc atcatcattt
    32461 agcagttaac gaatggggag gaaaaggtct ggcttcgcgt aagcaaggct taccaggttt
    32521 agcctactat gtcatcgaag tcgcacacaa aaaagaacta ttaacgattg cccaacgagc
    32581 acaagaagtt gacgcaccaa tcaaatggat gacatcgagc caattggaaa tcacagactc
    32641 agatggcatc gtgacccgta ttcgtttagc aaggtaaaat taggaatttt taataagtta
    32701 taagtattgc tagtttgctt tttgaacggt ctttggtaac agaactgcgt gagctggatg
    32761 ggcgagaatt ttgacaaaga agctgattag agatataatg aagaaaaatc gagcaaggaa
    32821 aaagaaatga caaattcaaa gtatattact tgtttgaaac gttcagaggg ccagttgtgt
    32881 gggattcaaa agatgattga aggagatcgt gactgtgctg atattgtgac gcaactgaca
    32941 gcagtgagat ctagtgtaga gcgcgtgatt gagatgataa ttaccgaaaa tcttactgaa
    33001 tgtatcaatc agccactaga tgattctgaa gctcaaaagg aacgcctaga aaaggctatc
    33061 cgatacttga ttaaacggaa ataaatgaat gttttgagac gatatgtgca aaggtctatt
    33121 aactattaga atagtttaat gtgagtcaaa agaagttgta aaaaatcttt aaaattaaaa
    33181 aaacgcatag tatcaggtgt tgaatgtact gacccccaaa agttagattt tttctgtcta
    33241 acttttgggg tgcagttcat aagaaccttg gtaatatgcg ttttattatg tagagactta
    33301 tactcttcga aaatctcttc aaaccacgtc agcttcacct tgccgtagat atggttactg
    33361 acttcgtcag ttctatccac aacctcaaaa cagtgttttg agctgacttt gtcagttcca
    33421 tccacaacct taaaacagtg ttttgagcag cctgcggcta acttcctagt ttgcacttta
    33481 attttcattg agtattactt catcttctca tgaaattgag tttttgccca gctcctcttg
    33541 attattctac ttgaccgggc caagcattca taccaccttc tacgttggta acggtgaggc
    33601 cttgggagct gagaaattgg caggtagatg cagagcggac tcctccttga caaatgacat
    33661 agtattcatg gtcaggtttg agttctttgt agccttgctc taaggtactt aatggaagat
    33721 ttttggcacc tggtgcatgt cctgcttgga attcatgtgc ttcacggaca tcgataagtt
    33781 ctagattttc attttggtat tttccataaa agtcagccat gctgatacta gtttccatat
    33841 tctactcctt ctgggttagc cattttgtat agtgaataag cgccgtcaag gttttggacg
    33901 gtaaatcctg cttgcttgag gataggctct gcgatatagc tgcgcaaacc actgtggcag
    33961 ctgacgatgt aggcttgttt tttgtccagt tcatcagctt cctccttgac actttgtcag
    34021 tttttgatac aatagtacaa aattagagga ggcaggctat gattcagaaa catgcgattc
    34081 ctattttaga gtttgatgac aatcctcagg cggttatcat gcccaatcac gaggggctgg
    34141 acttgcagtt gccaaagaag tgtgtttatg catttttagg tgaggagatt gaccgctatg
    34201 caagggaagt aggggcggat tgtgtcggcg aattcgtttc tgccaccaag acctatccag
    34261 tttatgtcgt gaactacaag gacgaggaga tttgtctggt tcaggctcct gtcggttcag
    34321 ctccagcagc ccagtttatg gattggttga ttggctatgg tgtggagcag attatctcta
    34381 ctgggacctg tggtgtccta gctgatatag aggaaaatgc ctttctagtc cctgttcgcg
    34441 ctttgcgaga tgagggagcc agttaccact atgtggcacc ttgtcgttat atggaaatgc
    34501 agccagaggc tattgctgct attgaggaag ttttggaaga cagagggatt ccttatgaag
    34561 aagtcatgac ctggacgaca gacggttttt accgagaaac ggctgaaaag gtggcttatc
    34621 gtaaggaaga aggctgtgct gttgtggaga tggagtgttc tgctcttgcg gcagtagctc
    34681 aattgcgtgg ggttctctgg ggtgaattgt tgttcacagc agattctcta gcggacttgg
    34741 accagtacga cagtcgtgac tggggctcgg aagcttttaa taaggcgcta gaactgagtt
    34801 tagcaagtgt tcaccacctt tagttgtact ggcaaaggat ttgttttatc ataaaatgtc
    34861 tagctcatac ttttcaaaaa tatgtttaaa cgaggtcacc ttcctcttgt cctaggcatg
    34921 ttgaggttgg gaaaaatctt taaaatcaga aaaacgtatc atatcaggtg atgaaaactt
    34981 tgatactatg cgttttatgt tggtaagatt tagagtgaga tgaaatgata ctcttcgaaa
    35041 atctcttcaa accaggtcag cttcaccttg ccgtatagat gttactgact tcgtcagttc
    35101 tatctgcaac ctcaaaacag tgttttgagc aacctgtgac tagctttcta agcgatgcct
    35161 tggttctcat tgcctataat caaaaagaga aattttctcc tgaaaagcat atagagtagc
    35221 tggcgttaaa agctcctgtc ttgctttttt gacctatagt cacatctatc aagtattgtt
    35281 cttgcctaag ctatcaataa aaaggtggca ttttttaggc ttggtgttag tagattttgc
    35341 cttatcctat ctaagtcatt tcgaactttt tatggtacaa tggaaacatg ttattcaaat
    35401 tatctaagga aaaaatagag ctaggcttat ctcgtttatc gccagcccgt cgtatttttt
    35461 tgagttttgc cttggtcatt ttactaggct ctcttctttt gagcttgccc tttgtccaag
    35521 ttgaaagctc acgagcgact tattttgatc atcttttcac tgctgtctct gcagtctgtg
    35581 tgacaggtct ctcaaccctt ccagtagctc acacctataa tatatggggc caaataatct
    35641 gtttgctctt gattcagatc ggtggtctag ggctcatgac ctttattggg gttttctata
    35701 tccagagcaa gcaaaagctt agtcttcgta gccgtgcaac tattcaggat agttttagtt
    35761 atggagaaac tcgatctttg agaaagtttg tctattctat ttttctcacg acctttttgg
    35821 ttgagagctt gggagctatt ttgcttagtt ttcgccttat tcctcaactt ggctggggac
    35881 gtggtctttt tagttccatt tttctagcga tctcagcctt ctgtaatgcc ggttttgata
    35941 atttagggag caccagttta tttgcttttc agaccgattt actggtcaat ctggtgattg
    36001 caggcttgat tattacaggc ggccttggtt ttatggtctg gtttgatttg gctggtcatg
    36061 taggaagaaa gaaaaaagga cgtctgcact ttcatacgaa gcttgtacta ttgttgacta
    36121 taggtttgtt gttatttgga acggcaacta ctctctttct tgagtggaac aatgctggaa
    36181 cgattggcaa tctccctgtt gccgataagg ttttagttag cttttttcaa acagtgacga
    36241 tgcgaacagc tggcttttct acgatagatt atactcaggc tcatcctgtg actcttttga
    36301 tttatatctt acagatgttt ctaggtgggg cacctggagg aacagctggg ggactcaaga
    36361 ttacgacatt ttttgtcctc ttggtctttg cacgaagtga gcttctaggc ttgcctcatg
    36421 ccaatgttgc gagacgaacg attgcgccgc gaacggttca aaaatccttt agtgtcttta
    36481 ttatcttttt gatgagcttc ttgataggat tgattctgct agggataaca gccaaaggca
    36541 atcctccctt tatccacctc atatttgaaa ccatttcagc tcttagtaca gttggtgtaa
    36601 cggcaaatct gactcctgac cttgggaaat tggctctcag tgttatcatg ccacttatgt
    36661 ttatgggacg aattggtccc ttgaccttgt ttgttagctt ggcagattac catccagaaa
    36721 agaaagatat gattcactat atgaaagcag atattagtat tggttaagaa aggaaagagc
    36781 atgtcagatc gtacgattgg aattttgggc ttgggaattt ttgggagcag tgtcctagct
    36841 gccctagcca agcaggatat gaatattatc gctattgatg accacgcaga gcgcatcaat
    36901 cagtttgagc cagttttggc gcgtggagtg attggtgaca tcacagatga agaattattg
    36961 agatcagcag ggattgatac ctgcgatacc gttgtagtcg cgacaggtga aaatctggag
    37021 tcgagtgtgc ttgcggttat gcactgtaag agtttggggg taccgactgt tattgctaag
    37081 gtcaaaagtc agaccgctaa gaaagtgcta gaaaagattg gagctgactc ggttatctcg
    37141 ccagagtatg aaatggggca gtctctagca cagaccattc ttttccataa tagtgttgat
    37201 gtctttcagt tggataaaaa tgtgtctatc gtggagatga aaattcctca gtcttgggca
    37261 ggtcaaagtc tgagtaaatt agacctccgt ggcaaataca atctgaatat tttgggtttc
    37321 cgagagcagg aaaattcccc attggatgtt gaatttggac cagatgacct cttgaaagca
    37381 gatacctata ttttggcagt catcaacaac cagtatttgg ataccctagt agcattgaat
    37441 tcgtaaagag ggatgacccc tcttttttga tgcccaagat ggcaaataga gacagaagcc
    37501 ccttgtcttc tagtaaaagt tcttcaaagg ctggacttta tggtaaaata gaaggaagtg
    37561 acaagagaga gtaatactca atgaaaatca aagatcaaac taggaaacta gctgcgggc
//
LOCUS       DAVBDG010000015        37495 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBDG010000015 DAVBDG010000000
VERSION     DAVBDG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.015"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(54..722)
                     /locus_tag="VQ526_000700"
     CDS             complement(54..722)
                     /locus_tag="VQ526_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2554451.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(833..1624)
                     /locus_tag="VQ526_000701"
     CDS             complement(833..1624)
                     /locus_tag="VQ526_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2554452.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEVVLADGGLDKEIDKVGLSTLERSFRALIYANLLSADANQ
                     QSVFYQELNAGFRNVLLNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSILFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            complement(2048..2812)
                     /locus_tag="VQ526_000702"
     CDS             complement(2048..2812)
                     /locus_tag="VQ526_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554453.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(2805..3575)
                     /locus_tag="VQ526_000703"
     CDS             complement(2805..3575)
                     /locus_tag="VQ526_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554454.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGDGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFWGLTGTEKEDLHV"
     gene            3822..4499
                     /locus_tag="VQ526_000704"
     CDS             3822..4499
                     /locus_tag="VQ526_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2554455.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
     gene            complement(4559..6355)
                     /gene="pepF"
                     /locus_tag="VQ526_000705"
     CDS             complement(4559..6355)
                     /gene="pepF"
                     /locus_tag="VQ526_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2554456.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETA
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(6366..7109)
                     /locus_tag="VQ526_000706"
     CDS             complement(6366..7109)
                     /locus_tag="VQ526_000706"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2554457.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESGKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(7111..8064)
                     /locus_tag="VQ526_000707"
     CDS             complement(7111..8064)
                     /locus_tag="VQ526_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2554458.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTAL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SIFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNP
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDK
                     WDMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            complement(8199..8627)
                     /locus_tag="VQ526_000708"
     CDS             complement(8199..8627)
                     /locus_tag="VQ526_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2554459.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(8608..9696)
                     /locus_tag="VQ526_000709"
     CDS             complement(8608..9696)
                     /locus_tag="VQ526_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2554460.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(9715..10185)
                     /locus_tag="VQ526_000710"
     CDS             complement(9715..10185)
                     /locus_tag="VQ526_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2554461.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(10476..10928)
                     /locus_tag="VQ526_000711"
     CDS             complement(10476..10928)
                     /locus_tag="VQ526_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2554462.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(10965..11143)
                     /locus_tag="VQ526_000712"
                     /pseudo
     CDS             complement(10965..11143)
                     /locus_tag="VQ526_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(11280..11459)
                     /locus_tag="VQ526_000713"
     CDS             complement(11280..11459)
                     /locus_tag="VQ526_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554463.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            complement(11624..11863)
                     /locus_tag="VQ526_000714"
     CDS             complement(11624..11863)
                     /locus_tag="VQ526_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554464.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            12133..13404
                     /locus_tag="VQ526_000715"
     CDS             12133..13404
                     /locus_tag="VQ526_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2554465.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            13669..13741
                     /locus_tag="VQ526_000716"
     tRNA            13669..13741
                     /locus_tag="VQ526_000716"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13702..13704,aa:Lys,seq:ctt)
     gene            complement(13824..14951)
                     /locus_tag="VQ526_000717"
     CDS             complement(13824..14951)
                     /locus_tag="VQ526_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2554466.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSARSVVTKKKYLMRRKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGKTVSRNFLGRLNSCIRASIQMAIADKVIIEDFTAYVELFSSK
                     SGQKVEEKYLHAESDYQKVLVYLKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDE
                     VDVVGEQLKTYRRYNTAIHKFTPPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELN
                     IDNENNLVFQHFGYAYDVPDIATVNKAIKVMLKELQISPLITTKGARHTYGSYLWHNN
                     IDLGVIAKILGHKDISMLVDVYGHTLQEKISEEFMTVKSLL"
     gene            complement(14953..15135)
                     /locus_tag="VQ526_000718"
     CDS             complement(14953..15135)
                     /locus_tag="VQ526_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HET2554467.1"
                     /translation="MKDTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            complement(15151..16194)
                     /locus_tag="VQ526_000719"
     CDS             complement(15151..16194)
                     /locus_tag="VQ526_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HET2554468.1"
                     /translation="MDKQVFNRAPSNSPITIHQMSNKSYSKFQEEVSLKYGFIGLKLD
                     KLSLTAEVSEEFHSEILSGNFTLYDYFGIVEPNLNRNGELASYKGQFFNDEKENWYIE
                     YTPVASIKMNKRPLKIEFTPSKVSKKNFLIVFHRMFPYMFNIAISTFHLAYDFERDLS
                     ALRVNWPKVMYRPIYKGMKLETMDFGAPKGNYHLTAYNKLKERMDSGDMAEIEIYQQY
                     DNLWRIEYKFYNEGNIKKELKNGLPFLAKIPVYIENFKGLEFNNLGVNEKIYLFALRN
                     KPELFAESDKRTVAKYKKLAESISEVNLNVFFQNALDFVEIFNQEPCLINFFEFMNSM
                     LQGDIPKLTINQE"
     gene            complement(16328..16591)
                     /locus_tag="VQ526_000720"
     CDS             complement(16328..16591)
                     /locus_tag="VQ526_000720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554469.1"
                     /translation="MKETVVVLAISTKKDRGWIRVSTANNCWSDLGMHFDKSKFGAVF
                     SAPGLYEVEVVNNASFGQNAQYEVTQVRKIGTFEELIEMAKIK"
     gene            complement(16767..18074)
                     /locus_tag="VQ526_000721"
     CDS             complement(16767..18074)
                     /locus_tag="VQ526_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HET2554470.1"
                     /translation="MFKIKQTASLSEHYFLNTSKLRSIRWFTIGFLSILSLSSSILFK
                     GWFLQMITLFGELIIILYIERKIKGWAQQIEPLLIVRENLLFMLRRNSFLFTATKDGA
                     ILRSAKFNYQLNDVSIVIYAFKSGDEFTKEMDNLDVLLSSALGISLSSKEIYATHVEY
                     VFVYRQPERLHVTSLPLEDDSLKIKIYDDFVIDLRKNFSMLISGASGSGKSFFTYYYL
                     TRFISQTVNGKHAKIYVIDPKFLDIYKLCEFSGFPVENYGTTNEDAFRIVRHYINEMN
                     RRMEIYNKSDLFDSICIDLGFPPLLLVIEEYSSLLASMDAKAKKDFENMVAIVAQKAR
                     SLSMGVCIVMQQPRSDSLSTNIREQLVNAIFLGAPTRESSQMMFGTTDVPKVKKDKGV
                     GLYSTDREPPKEFHSPMFDRDVFEAIFPVWKWATKDYMKDEDV"
     gene            complement(18290..18511)
                     /locus_tag="VQ526_000722"
     CDS             complement(18290..18511)
                     /locus_tag="VQ526_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554471.1"
                     /translation="MLKNFCQNAFISLYFIVSLLGGFSAQVDLKSFSPYQSMKIALLL
                     GVTLILLQLPIMFAKLLEALKTKKGETHE"
     gene            complement(18565..18864)
                     /locus_tag="VQ526_000723"
     CDS             complement(18565..18864)
                     /locus_tag="VQ526_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554472.1"
                     /translation="MNRNVLEFLKTETAEKISLFIRKINGLEGNVTLLSINSQDLEDI
                     KNAMLSNSNLGLKIARLDVMKKIAYASNRTHYKDGTTIMDDISSGKIHRRPKSYI"
     gene            complement(19272..19634)
                     /locus_tag="VQ526_000724"
     CDS             complement(19272..19634)
                     /locus_tag="VQ526_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554473.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
     gene            complement(20182..21501)
                     /locus_tag="VQ526_000725"
     CDS             complement(20182..21501)
                     /locus_tag="VQ526_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2554474.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNVQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(21511..22356)
                     /locus_tag="VQ526_000726"
     CDS             complement(21511..22356)
                     /locus_tag="VQ526_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2554475.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            complement(22358..23260)
                     /locus_tag="VQ526_000727"
     CDS             complement(22358..23260)
                     /locus_tag="VQ526_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2554476.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMINLVQRRVLKEDD"
     gene            complement(23356..24666)
                     /locus_tag="VQ526_000728"
     CDS             complement(23356..24666)
                     /locus_tag="VQ526_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2554477.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(24793..25788)
                     /locus_tag="VQ526_000729"
     CDS             complement(24793..25788)
                     /locus_tag="VQ526_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2554478.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI"
     gene            complement(26150..27631)
                     /locus_tag="VQ526_000730"
     CDS             complement(26150..27631)
                     /locus_tag="VQ526_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2554479.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            28074..28238
                     /locus_tag="VQ526_000731"
     CDS             28074..28238
                     /locus_tag="VQ526_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2554480.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIIGAAIVI
                     TVVSFFTGRK"
     gene            28322..28888
                     /gene="amaP"
                     /locus_tag="VQ526_000732"
     CDS             28322..28888
                     /gene="amaP"
                     /locus_tag="VQ526_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2554481.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            28900..29070
                     /locus_tag="VQ526_000733"
     CDS             28900..29070
                     /locus_tag="VQ526_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2554482.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            29109..29717
                     /locus_tag="VQ526_000734"
     CDS             29109..29717
                     /locus_tag="VQ526_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2554483.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            29748..29951
                     /locus_tag="VQ526_000735"
     CDS             29748..29951
                     /locus_tag="VQ526_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2554484.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(30061..30333)
                     /locus_tag="VQ526_000736"
                     /pseudo
     CDS             complement(30061..30333)
                     /locus_tag="VQ526_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30537..30824
                     /locus_tag="VQ526_000737"
     CDS             30537..30824
                     /locus_tag="VQ526_000737"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2554485.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(31102..31872)
                     /locus_tag="VQ526_000738"
     CDS             complement(31102..31872)
                     /locus_tag="VQ526_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2554486.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(31887..32513)
                     /locus_tag="VQ526_000739"
                     /pseudo
     CDS             complement(31887..32513)
                     /locus_tag="VQ526_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            complement(32419..33021)
                     /locus_tag="VQ526_000740"
     CDS             complement(32419..33021)
                     /locus_tag="VQ526_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2554487.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGRKGGISSSCWGSVVVTSSERLLETSLR"
     gene            complement(33031..33347)
                     /locus_tag="VQ526_000741"
                     /pseudo
     CDS             complement(33031..33347)
                     /locus_tag="VQ526_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
     gene            complement(33364..33681)
                     /locus_tag="VQ526_000742"
     CDS             complement(33364..33681)
                     /locus_tag="VQ526_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2554488.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(33696..34592)
                     /locus_tag="VQ526_000743"
     CDS             complement(33696..34592)
                     /locus_tag="VQ526_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2554489.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(34595..35857)
                     /locus_tag="VQ526_000744"
     CDS             complement(34595..35857)
                     /locus_tag="VQ526_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2554490.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWSVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDSGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            36137..37315
                     /locus_tag="VQ526_000745"
     CDS             36137..37315
                     /locus_tag="VQ526_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2554491.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT        12230 a         7718 c         5933 g        11614 t
ORIGIN      
        1 atacgaaaaa gccctgttcc tcaagatgag gagcaaggct ttttgagttt caattattct
       61 tctgttccaa ggaaattttt tgcaacaagg gctgcaagaa ctccaccagc gattggtgca
      121 aggatgaaaa tccaaacttg ttgaagggct gcgccgccta ccaagacagc tggtgccaag
      181 ctacgagctg ggtttactga aagtccagta atcttcaatc cgacaagaat catcgccatc
      241 aatgacaaac cgattaccaa accagcaatc gcgccattgc ccttactttc tgaagtcaca
      301 gtcatgataa ccaagacaaa caagaaagtt gcgatgactt caaacaagaa accaccaaag
      361 acagtgacac cgtttgccaa ggcattttca ccaaggctag cagttgacat acctgaatta
      421 cccaagagga agaagactgc gccagaagcg atgaaagctc caacaacctg accaaggatg
      481 tagtttacaa gttctgaaga tgacaaacgt ttgtttacaa acatagcaat cgaaacagcc
      541 gggttcaagt gagcacctga aacagttccg attgagtagg ctgccaccac gattgccaaa
      601 ccaaaggcaa aggcgattcc aaggtgacca aggccatcaa gaccatttcc aaaaacaaca
      661 gctcctgtcc caacgaacac aagcatgaac gtaccgatta actcagcaac aaattttttc
      721 attttctttc tccttttttc aaaaactaga tactagtcta tcaaaagtag gaaagggttt
      781 caagaaaatt gattggaaat tttttgaaaa tcatagaact attagctaat ctctagtatt
      841 gaaaagactg gatagcttct ttcaagtcat cttgtaaact atttctctgg tcaagttgga
      901 catagacttc caccagacag gatctaaagt tggaaaattt gtaaaaatcc tccctttctt
      961 ctatcggaaa atcaacagtt tttatccaag aagctacttg ttcttgctcc aacttccctt
     1021 gtaaaatagg ttcatagatc actcttgcta aacgccaatc ctcatcatct gtaaagagaa
     1081 tcgacattct tttaaatagt tggccaagta tatcaaatac ttcatgaact ctgtttttag
     1141 gaaagtctgg atgacaaacc acctctgtca gtaaatcggc tccatgtgca aaagcgtgaa
     1201 cccaaccata ctgacttgag aaacctgttg tatccttttc ttttgaaaga tagtgcaagc
     1261 cttgatttaa aaggacatta cgaaatcctg catttaattc ctgataaaaa accgattgct
     1321 ggttggcatc tgcagacaag agatttgcat aaataagtgc cctaaaagaa cgttcaagtg
     1381 ttgacaagcc taccttatca atctctttat ctaaccctcc atcagctaag accacctcag
     1441 caatgaaatg aaattgctcc tgtgtaaata gctcttcctg aatccctcta gcaaagcttg
     1501 taaaaacaag atcatcacga atttctggag aaggatctcc caaatgatca agcaaccact
     1561 ggatttcctc ctggttataa tttggttttt cttctgctat ttttcttagt aaatcttgat
     1621 acatggtcaa tacctctaca tttctagcaa ctgttcaaaa aggcagtctt aaatgacctt
     1681 accaatttgt aaaattaagt taaaaagaaa aagacattta tattcagaga aacagcatat
     1741 ttctgaaaag atatataaat gatttattcc atattacaac aaataaactt gtaatgatag
     1801 aaccttatca caaaaatcac attcctgtct caaaaattgc gaaaaactag ttataataaa
     1861 gtgaaataaa aactatacaa attgattagg gaatttaaaa caatttctaa caatatttaa
     1921 aaactgaacc gtactatttc aacttccata aattacaata ttgataggca cagttcccat
     1981 acctgtccaa attcgaataa cgtttagtat aaattagaat atgactacaa catcacaaat
     2041 aattagacta tctataatat ctacgatgta acatatacat aggaagcacc gttatcaaga
     2101 gcgccaaggc tatcaaccag ataagatgaa gatacaaacc tgtcacctca cctccccata
     2161 taatacgaat catgcgggta gcgtgataca agggagaaaa ccaagtcaaa tcaccaagta
     2221 ttgggggcaa ggaagtaatg ggaaagaagg tatctgaaaa catataaaga ggacggatta
     2281 ccaaaacatt gtagtaattc agaaattccc tagacggcgc caagtaggta aagattagtc
     2341 ccaaaacacc aaacaaataa cccactataa ataaaatcag aggaatcaat aacatcaaga
     2401 catgaaaatg cgctgtgata agcatggaaa tcagccaaaa aatcccaccg aaaatcactg
     2461 cacgaatacc tgcccagata gcctgaccta ccacaatatc attcgtattg attggcgtca
     2521 tcaacatagc atcataagtc ttttcaatct tatactggat aaaggcattg accgttgttt
     2581 ctgcggtggc cgcagacatg gcagtcgcag caattaaacc tgtaatgaca aaatcacggt
     2641 agagcatacc gttaacatgc gtcaaataag caccaactcc aaatcccatt gctaaaagat
     2701 aaaagagggg gtcgataagg ttggggacaa ttgaaatttt aaataaattt ctaaaactca
     2761 tgtagttgcg taccacaacg tgtccgatgg agtgaaatga taacttaaac atgcaagtcc
     2821 tccttctctg tcccagtcaa cccccaaaaa agcgtctcaa ggttgtcagc atcatacttg
     2881 atgagcaagt cttctacagt tccttcttct ttgatagtcc cttggtggag catcagcacc
     2941 ctatctgtca aatattcaat ctcattcata tagtgggaag taataatgat agaaacacct
     3001 aattccttca attccaaaag tttatcccaa ataatattgc gggattgaat gtctaaacca
     3061 actgtcggtt catctaaaat gataatatca ggccgattga tgagagctct tgccaagaca
     3121 agacgtcgtc gcatcccacc cgacagctga ttcaccatct tttgcgcatg tttgctcagt
     3181 tgaacaaaat ccaagagaga atcgattctc ttcctaagtt cgacagatga gataccataa
     3241 catagaccgt gagcaaaaag attatcataa acattcaagg tcatgtccag catatcctcc
     3301 tgccctacaa tacccagaca agatttactc ttacgacgat tggtttcaat attttgcccc
     3361 aagatatcaa tctctcctga ggaaattgag gtcagtgagt aaatcatctt taaagtggat
     3421 gttttgccag caccgttgat tcccaagata cccaaacact ctccgtctcc caattcaaag
     3481 ctaattccct tgacagcttc ataagaacca tattgctttt ttagattttt aacacttaac
     3541 ttcttcactc tttctttccc ccacaaatag agcatgttgg atactttgtt aattcatctc
     3601 acttttgaaa aaatgttgag aatgtaatct taagattaac gtccttcaac tgtggatact
     3661 gctgcaatta cttttgtaat tttacctcca tttaaactca gacaataaaa tttaaaatat
     3721 tctctagtaa aacattttta aaatctattt ttcttgtgct ttaattatag tataataaaa
     3781 gatagaaaat catcgatata tgaaatttgg agaaaatttt tatggaacca gatattttca
     3841 tcatcacaaa actcttatca gacccttctc ggatgcacat tttagatatt ctaatggatg
     3901 gaaaagctca cacggttaac gaaattgcca gctttaccaa aatcaagcaa catactgtca
     3961 gctatcacct caaattactt accgaagcgc aggtaaccac tctacaaacc tatggacgtt
     4021 ttcattatta ttctttaaaa agtgctgcca ttgcagaaat gctggaattt ctcagcttct
     4081 actctcctca gcgtgatgtc aaaagttata aacaacacat tcacaaaaag gaattaaagg
     4141 tcgctcgcac ctgctatgac catattgctg gtgagcttgg catttccatt accaattatc
     4201 tcttgcaaga aaatctcctc agcgaatcag aaaaagactt ccaactcact gaagctggga
     4261 agtcttattt ccaaagaaaa ttacacattg atacggatga attgaaaaag aaaaaacgga
     4321 aattttgccc taaatgtttg gattggagcg aacgtaaaaa tcatgtcggc ggagctctcg
     4381 gcaatgctct tttagaattt ttcagtgaaa aacaattggt catccctgca caaacgccac
     4441 gttcacttac cattacagag aagggcaaag aattcctcga aaaggaatgg gggatttaaa
     4501 catggacttt taagcaaata actgtccata cccattctat aacaacctct ctatcccctt
     4561 actcccccaa ctcagcactg taggcgataa tctggtcaac tgtgtcagac aagaattgga
     4621 tggtatcacg gagtggtttg tctgttgaaa tatcagcacc gataatcatg gctgactcaa
     4681 gtggtgtctt gctaccacct gatttgagga gattgagcca gtcttcagct ccagtttctg
     4741 agtttttaag atgaaggtaa ccagctgtcg agatgacaag accagcagag taagtgtaac
     4801 tatacaagcc catatagtag tgagcttggc gcatccaagt caaagctgca tcgtcgtcga
     4861 tttcaatggc atcgccccaa aaatctgtca aaacttcctt cataatgctg ttgagtttgc
     4921 ttgctccgaa ggtttctcct tcttcaatca atgtatacac cttacgctgg aaggcggctt
     4981 ccaagaggtg ggtgatgaaa ttatggaagt aggtgtctgt caagcgatga gccagagcga
     5041 agcgtttttg acgtgggtca ttagactggt tctctaagta atcactgagt agcaattcat
     5101 tgaaggtcga cggtgcttca acataatagg tcgacatgtg ggcgttgaaa tagctttggt
     5161 gattgtctga aaagatgaat tgaccagaat gcccgatttc atgaatcaag gtatagacat
     5221 cgctcaaacg gcctgtccag ctcatgagta cataagggtg tacgcgatat gggtccgccg
     5281 cataaccacc ggaatccttg ccactgttag cagcaaagtc cacccagcgc tcttcttggt
     5341 aacgagcaac ttcctgacaa tattcttgcc ccaaaggttc taccgacttc atgaccaaat
     5401 cataggcatc gtcaatagtc acttcaggat tgagggcgct gtccaagtcc aatttccagt
     5461 ctgcaaaggt catcttttca agaccattta ccttggcaac atgcttgagg tatctctgag
     5521 cgactggtgc aaagtccttc atgatgaggt caatctggcg atcaaacatg acacggtcca
     5581 cttcttgttc agctagaaga tagtcaaaga cagagtcata tcccttcata tcagccaaga
     5641 gtttttcaga cttgacctga gccagatagg ctgctgcagc cgtattttgg tgcttacgaa
     5701 gtccctctga gaaggaacgg aaggatttct cacgaacctc agcatcctcg tgattttggt
     5761 agaaattctc ataggtgaca aagctgtttt tataggtctt gccatgggct tcaaagtcag
     5821 ccatttcaaa atccccagct cgcatcttag tataaatgtc ctgcggacta tagaaaactt
     5881 caccgagatt tgtcaaggcc ttctccacat ctgcccctaa gtagtgggct tttttgattt
     5941 tagcctgacg aatggcagct gttaaatgtg gcaatttacc caaacggtcc aagacttcct
     6001 cgtctgctgc caccaaggca tcgtcaaaga aggtcaaggc tacgctggca gctgtttcaa
     6061 attccatccc agcttgggca atattggcaa attcgtcatt gctatagtcc gtcgtctgag
     6121 gcataaaagc atagttgcca atatggctca tctgaatgta gatctgttcc aattccgcaa
     6181 aggccttctc gaaatcctca aaagtgtgaa gattgccctt gtaatcacgg ctaaactggt
     6241 tgatgtcttc gcgagctttc tcgattgcac gcaagaaatc ctcacggtct tggtataggg
     6301 ctgttaagtc ccagagttcc ttctctggaa attctgaacg gtgtttttgt tccattttct
     6361 tcctcttatt tctctaattc tactaaaaca ctaagggctg ataaggcgta aagcggtgct
     6421 gtttctgctc gcaaaatacg aggacctagg cctaccaaaa cggctccctt agcttcaaaa
     6481 ctttcgattt ctgcaggtga gagaccgcct tctggaccaa agataaagag cagtttggct
     6541 cctgtttcaa gaccagtgac tgcttgcaga agcgcagcgg cttctccttc tttacctgat
     6601 tcttcatagg ctactatgat agagtcaaac tggtccagct gagttagaaa atctgctttt
     6661 ttctcgaaaa gtttaatact tggtacaata ttacgcttgc tttgctcggc tgctccaagg
     6721 gcaatttttt ctagtttttc aactttttta cccaatttct tgccatccca cttggcaact
     6781 gaccagtctg caggaaaggc ccagatttgg ctagcaccca gttcggttac tttttgagcg
     6841 atgaactcca gcttgtctcc cttgggaaat ccagatgcga tggtcacttg aactggtagt
     6901 tccacattgt catttaattc ttggaccaac tcaaactgac gattttccat atccagcacg
     6961 cgcgccaagc gcttgatgcc atcatcaaag actaaggtaa cctcatcctc ttctttcaag
     7021 cgcataacct gaaacatatg cttactggtt tccttgttct cgatagtgac aggagagata
     7081 gcactgcctt ttacaaaata ctgctgcatg ctagcctcca atcacaccag agatatcctt
     7141 ggttttctta aagacacagg cattccattc cccttgaacc atgtgagttt cgaggaaaaa
     7201 tccagctgat tcagccaact cgcgcaccat gtcccactta tccttgataa tgccactcat
     7261 gatcaggtaa ccttcatcct taaccaagcg ataagcatcc tctgtcagat gaatgaggat
     7321 atccgccaag atgtttgcca cgatgacatc tgcttcaatc tccacgcctt taagcaaatc
     7381 gcctgccgct acatggatat tttccattcc agggttgagc tcaatatttt cctgagccac
     7441 acgaactgcc acatcatcca ggtcataggc gaaaatttct ttagccccca gaagcgagct
     7501 ggcaatagag agaacccctg aaccagttcc cacatctagc accgtttcgc caccacgaag
     7561 aacctgttcc aaggcaaaaa tgctcatctt ggtagttggg tgggttcccg taccaaaagc
     7621 catgccagga tccagcttga taattttttc tcccgcagtc gcctcatagt ctgtccaaga
     7681 gggaacgatg gtcaaatcat gagtgatacg agctggctca tagtatttct tccagttatc
     7741 tgcccagtct tcctcagcca aggcagtcgt acctattttt aactctccca aatccataaa
     7801 atccgtcaat tctgatatgc gagcctgcaa gtccgcctca accactgtca catccaccgt
     7861 gtcagggtag taggctgtca ctacgatttc ttcttgctgc tcgacctctg ggaaaatctc
     7921 gccgaagcgg tcaacatttc ccacatagtc catactgtct tcaatcgcaa caccttgcgc
     7981 tcccagctca atcaagagat tggaaaccaa ctcctctccc tcacgcttca ctgtaacttt
     8041 taactcttgc catgtttcca tcattaagat accaagcccg taaaacacaa aaccaaaata
     8101 ggaaattctc tgaagacgct tgtgtctaag agaagtttat ctttttggca cagtgtttag
     8161 ggcgggttca gtttagaaat ttaactgaac catcctttct aatcacttac ttttaaataa
     8221 tcttttaatc tctcttgcaa ctgaggcaca acttgactgg aactaagaaa ttcctcaaca
     8281 ttcatcagct gatagccctg tccttcatct ccgaagatga tattgtcaaa ttgttcttgt
     8341 cttagctgac caaccataaa gaccgatttc ttgcctttaa aaattacgct aggataaatc
     8401 ttgctccaaa gcagacagtc ttcatctaaa tgaattccca gttcctcata aacttcacgc
     8461 cgagcgcatt caaaagggct ttcgtcccct tcacggccac cacctggcaa ctcccacatg
     8521 ttgggacaag ggatatcgtc cttgtcatca cgcaaaatcg tcagtagctt atccccacaa
     8581 aagagggcaa tcttgcaacc ttggaaatca gaaatttcta gttccatctt cagttccttc
     8641 taacatttcc ttttccagct ctgctaacaa gttttcataa tagcgtttct cgtccctcaa
     8701 cattcgacta ctatccattt tctgtctagc aatcttgaga gccttacgag ttcgatctac
     8761 atctttcttc acctttaatt gataccaggc ttgtatcact tgaagattgg acagtttgag
     8821 agacagaaac gatttgacct gtcgaatact agcatattgc tccgcttgct caaaatctcc
     8881 ttccaacaag gcgatatgaa gcagggatag ttgggcaact gtctgcatca tcggagtagt
     8941 tgtcctctca agtaatgctt gaaactgctg tttagctact tcttccttcc cttccaaaat
     9001 ggaaacttca ccttgcatac ctaatacacc atccgcaaaa ctccctcgtg catcctcagg
     9061 aactgcttga acaaagtctt tcaaatcata ttcttgagga gccagcaaag tttgagcaga
     9121 atgtctcaat accaggtagg cgtatttggt attttcaggg tgttgtagta attcccaaat
     9181 ttttgctcca tcggtgatgt cgactggcaa aatgttattt aggaagaaag ataaattaag
     9241 aaaaatccaa gtccctgcaa aataccagct tcttgtcaaa aagccaaaca ctatcgccaa
     9301 taatatcaag ccgagatgaa ccatcaagcc tcctgaaagc atcaggatga ttctttgatc
     9361 gctttcatcc tcttttaaac caatgtattg agcaccaaca tttttcagaa tggctgttct
     9421 actaagatga aacctgcctg actttttggt caaaataaaa tgtcctaatc caaaagccac
     9481 cagccgatag cctgtcaagt agccacaaaa agcatgaccc agctcatgaa gaataaagat
     9541 taaatacatg cttagaagag cgaaggcata accaaaagta aaggctaaaa ctgcggaata
     9601 ccccaactct gcaaatgcga ttgttccaca agcaaaagct agcataataa agacaacagc
     9661 tagcacataa accaaataag tcccaatttt cttcataaca cctccaacca actcctagta
     9721 tcttggataa ggataaaatt ctccctcttc caagccaact tttccttctt caaagacttc
     9781 ttgattccat tccataacaa attcctctgc ttctggatct tccaaaaagt ccatgagggt
     9841 atctagccca acctccgcag tatctttgag gaaaagggca aaataagcta aaaactcgcg
     9901 agaaaatcct tttttaggta gataagggat aaccgttaaa tagtcttctg cattgacggt
     9961 tgacttggca ggattgtaga aaaggaccgc ttcctcaaaa agaatgtcat ctgatgaaac
    10021 ctctccgtct tcatccacca tctccacacc tgcagcattt tgcgcttcca atagaaaact
    10081 cacttctacc gcatggttgc gtttgtccca gctaatctca aagtcaaagg gaaagttctt
    10141 gtccaactct tcctctaaaa catctaaaaa tccgtatgtt gccattttgt cctctttcta
    10201 tgcgactctt taatcgcccc gattgctcgg aaatatgcta aaatagatac taccatctta
    10261 ccacaaaatt attttatgtc ctaattatac catattacct catttaaacc cttggtatca
    10321 gtgattttct taaaagtctg atttcttcat ttctcataaa aatcaatata aaaagccctc
    10381 gaaagggcta ataaatctat aaaatcaata ggcgagtaac tagcacaagt ggacgtgctt
    10441 ttttattgac tattaccacg ataccacgct taatcttagg cttgaacttt cttatctgca
    10501 atagcgtctg tcaaagtctg agaaaagtta agtcccattt ctcgtcccaa cttatctgcc
    10561 cattttggta tggtcaaagt ctttttaatg ggttcctgac ttcctaggta ttctgataca
    10621 tcaacagata ccatagaaat aaaagattta tcaaggtcat aggttgacac gaaatcttca
    10681 tcatctttaa aaggatcatt atcaattaaa gacaagctat tgatatctga tggctgaggt
    10741 aactctccat cactctctat caaatctgca acagttatcc ctagccactc cgaccccata
    10801 gccaaagcct cagaaatccc ctctccttgt gtagctgagt attcaaaatc tgggaaatgg
    10861 acaaaataag tcgcttctgt tccgtctgtg tcgtcataat aaaataaagc tggatacgta
    10921 actaacattt cactacctcc atatcaaaaa gcagggactg aattttacaa cccagcttgc
    10981 tttcttatcc ctctttcagt gtacttattc agctcaccat gagggattgt gataggtctt
    11041 tccccttgct tctcgatttt aatatgggaa cctttaccgc ctctagtctt tatcaaccat
    11101 gggccgtaag gagtttaacc atctcttttt gtgtcatagg catagcgctt ttacctcctg
    11161 acaacaccat tataacacgt gttacacgta ttgtaaagga gtgatactta ttattctatt
    11221 atacataaaa gcccctagat gtggttctaa gggaagccaa tttattcata cctatttttc
    11281 taatgagtag taaaaactgc ttctttatcg agcaattcat catctgtata gtcaattgta
    11341 aaagtatctc gatctaagac agattgaggc ggagttgaat gaatcatagg aacactgcgt
    11401 actctatatt ttttatctcc aatttttaca aactgatact cttcgaaaat caaattcaaa
    11461 ccacgtcaac gtcgccttac cgtactcaag tacagcctgc ggctagtttc ctagtttgct
    11521 ctttgatttt cattgagtat gattaactct caagtcttcg aaatcaggat tttcaacagt
    11581 tattacaagg aggcgattta ctacttcaaa aacatcaatt attctatttt tcatattttt
    11641 tcaacccatt attagaatga acttcttggt aagcaaaatc aagtttagat ttaatgtttt
    11701 cgtacaaatc taaaatctct tttggagtat cttcccggaa gaaaagtttt cttttccctg
    11761 aaataacttg atcactaaga atccaatgac gaatttgttt tgtaaaaatc aaaatttcct
    11821 gacttggtcc atcatttcca ttgcttatca cctctctttt cattatagtt catacaatga
    11881 cattcagcaa tattatttct caagtcagca cttccacttc tttaggctca actatcctat
    11941 tttgagcttt aaggaaaatc aaatctctca tgctgatacc tctcctcatt aaattaaata
    12001 gtaaaaaaga ttctatctca ctccctgatt attacaaaac cattgaaata tcacaactaa
    12061 taggctagaa tggacatagt aagatatagt agatgagtca ttctactcaa atccacgtta
    12121 gaaaggactg ctatgccaga caatctcgcg cttcgcatgc gccctaaaac catcgaccag
    12181 gtcatcggtc aggagcatct ggtcggacct ggaaaaatca tccgccgcat ggtggaagcc
    12241 aaccgcctgt cctccatgat tctatatggc cctccgggaa tcggcaaaac cagtattgcc
    12301 tctgccatcg ctggaacgac caagtatgcc tttcgaactt tcaatgcgac agttgatagt
    12361 aaaaagcgac tgcaagaaat ctcggaagaa gctaaatttt ctggtggtct cgtcctattg
    12421 ctagacgaaa ttcatcgact agataagacc aagcaagact tcctcttgcc tctcttggaa
    12481 agtggactgg tcatcatgat tggagcaacg actgaaaatc ctttcttctc tgtcactcct
    12541 gccattcgta gccgtgttca aattttcgag ttggaacctc tgtctaacca agacgtcaaa
    12601 gaggccctgc agatagctct aagtaaccct gaacgtggtt ttgattttcc aatagaacta
    12661 gatgaggatg cgctggattt catcgcaacc tctacaaacg gagaccttcg ctctgccttt
    12721 aactcactgg acttggctgt tctatctacc cctgagaatg acgagggcat tcgccacatc
    12781 accttagaca tcatggaaaa tagtcttcag agaagctaca tcactatgga caaggatgga
    12841 gacggtcact atgatgttct atctgccctg caaaagtcta ttcgtggctc agatgtggat
    12901 gccagtctcc actatactgc ccgcttgatt gaggctgggg atctgcctag tctcgctcgt
    12961 cgcttgactg ttatcgccta tgaagatatc ggcttagcca atcctgaggc ccagattcat
    13021 accgtgactg ctctggatgc tgcacaaagg attggtttcc ccgaagcccg cattctcatt
    13081 gccaatgtcg tgattgattt ggccctttct ccaaaatcca actcagccta tgtagctatg
    13141 gataaggcac ttgctgacct caaaacatca gggcacttgc ctattccgcg acacctacgt
    13201 gatgggcact acagtggaag caaggaactg gggaatgccc aagactatct ctatccacac
    13261 aactatcctg gaaattgggt aaagcaagac tatctgccag aaaaaattcg taatcatcac
    13321 tatttccaag cagaagatac tggtaaatat gaacgggctt tggctcaaag aaaggaagct
    13381 atcgaccatt tgcgaaaaat ctgaaatcct tttcaaaaaa ttgcactttc ctcttgattt
    13441 tttttaaaaa agtggtatca tataaatata gaaacgctgt ggtgtacgac ttcacactta
    13501 agtgttgacc gactattttt tgtattatta gggaaacaaa agtcttctaa cagcatgtag
    13561 gccgtctcac acggaaacag cttcagttag agcgagttgc ccacctgctt aattgcgcgg
    13621 gttcaataca aaccgtgaag tttcggcacc aatacagctt ttttctttgc ctccttagct
    13681 cagctggcag agcagcggac tcttaatccg tgggtcacag gttcgatccc tgtagggggc
    13741 atataaatac aacaggaaaa gccttataat atagggcttt ttttgccttc cttttaaaaa
    13801 ttgtcgtgca atttgccgtg tttttacaac aaacttttca cagtcataaa ctcttcacta
    13861 attttttcct gcaatgtgtg accataaacg tcaaccagca tagagatatc tttatgtcct
    13921 aaaattttcg caataacacc caaatcaata ttattatgcc ataaataact accataagtg
    13981 tgtctagctc ctttggtagt aatcaatgga gaaatttgca attccttcaa cattaccttt
    14041 atagctttat tgactgttgc aatatctgga acatcgtagg cataaccaaa atgctgaaaa
    14101 accaaattat tttcattgtc aatgtttaat tctttatttg tttttaattg caatattttc
    14161 aatgttttta acaaggaaag catatctgaa gtaataggaa ctaatcttat tgaagtattg
    14221 tttttgggtg gagtaaactt atgaatagcg gtgttgtaac gacgataagt tttaagttgc
    14281 tctccaacca catcaacctc atcccaagtt agagcaatca attcagaaaa acgcatgcca
    14341 gttttaaaaa ggaaataaat tacatacggt acaatagatt tctgataatc aaacttattt
    14401 ttcaaatata ctaaaacttt ttgataatct gactcagcat gcaaatactt ttcctcaacc
    14461 ttttgtccac tcttagaaga aaacaactca acataagcag taaaatcctc tattatcacc
    14521 ttatcagcaa tagccatctg tatacttgcc cgaatacaag aattcaacct tcctaaaaaa
    14581 tttctcgata cagtttttcc atactcgttc ataatttttt gatactcgct aggcttaatt
    14641 tgtgatacag gcttatttcc aaatagcctt tcgatgacct tccttctcat aagatatttt
    14701 tttttagtaa ctacacttct agcactaggt aaaattttta aatcaagcca ttcattatac
    14761 agttccgata aagtcatact agagcttaag acactcccag tatttaactt atgaagaact
    14821 ttctccgctt ctattttagc ttctcgtttt gttttaaatc cacttttatg gagtaggctc
    14881 ttgctcctct cacgtataga gaacgaccaa agccttttct tcccacgctg tttatactcg
    14941 atataagcca tttcacaatt ccattcccaa caactctttc acaacacttt ttggaacaac
    15001 catcaaacgt ttgcgattat aaaatgtata gcctcgtgat acaagtaatt ctctcgcctg
    15061 atggataata gcattagctg ttgatggcct atagcccatg gaaatcaaat ccttattaga
    15121 aatcgtatct ttcatactgt tcccctctta ttattcttgg tttattgtta actttggaat
    15181 atcgccttgt aacatagaat tcataaactc aaaaaaattt atcagacaag gctcttgatt
    15241 aaagatttca acaaaatcta aagcattttg aaaaaagaca ttcaaattaa cttcagagat
    15301 actttcggca agttttttat acttagctac cgttcgttta tcagattctg caaataattc
    15361 aggtttatta cgaagagcga aaagataaat cttctcatta actcctaaat tattaaattc
    15421 taagcctttg aaattttcaa tatacactgg aattttagct aagaatggta atccattttt
    15481 taattctttt tttatgttgc cttcattgta gaatttatac tcaatcctcc atagattatc
    15541 atattgctga taaatttcta tttctgccat atctccacta tccatacgtt ctttaagttt
    15601 attataagca gttagatgat aattaccttt aggagcacca aaatccatag tctcaagttt
    15661 catcccctta taaataggac gatacattac ttttggccaa tttacacgta aagcagataa
    15721 atctctctca aaatcatagg ctaaatgaaa agtagagatt gcgatgttaa acatgtacgg
    15781 aaacattcta tgaaagacaa ttaaaaaatt cttttttgaa acttttgaag gagtaaattc
    15841 aatctttaaa ggtcttttgt tcatcttaat cgacgcaacg ggggtatact cgatatacca
    15901 attctctttt tcatcattaa agaattgccc tttataacta gctagttcac catttctatt
    15961 taaattcggc tcaactatac cgaaataatc atataaggta aagtttccac taagaatctc
    16021 agaatgaaat tcctcactaa cttcagcggt caaacttagc ttatccaatt tcagaccgat
    16081 aaacccatac ttcagagata cttcctcttg aaatttagag taactcttat tagacatttg
    16141 atgtatcgtt atcgggctat tagagggagc ccgattaaaa acttgtttat ccatgaaatt
    16201 tctccttcaa aaaacacaaa gttgaaacat tttatatact taaattttag ggcaagactg
    16261 ccgttccact gaccacgctc cacttgccag caaagctggc aggcccagtc ttaccctaaa
    16321 atataaatta tttaattttt gccatttcaa tcaattcttc aaaagtccca atctttctaa
    16381 cttgagttac ctcatattgg gcattttgac caaatgaagc attattaacg acttcaacct
    16441 cataaaggcc tggagcagaa aagacagctc caaatttgga tttatcgaaa tgcataccta
    16501 ggtcactcca acaattattg gctgtggaga ctctgatcca gcctcggtct ttcttcgtag
    16561 aaatagctag aacaacaaca gtttctttca tagttaaaaa atccttcctt atttaagtaa
    16621 tatacagctt ttaacgtcaa tcagtattgg acataaatac atttattcga acgttcgttc
    16681 gaccggcctt gttgttcgcc ggctgcacaa tggcattttg aaaatgaata aaagaatttt
    16741 attcatcttc aaaacgctta aaattctcat acatcttcat ccttcatata atccttggta
    16801 gcccacttcc acacaggaaa aatcgcctca aatacatctc tatcaaacat cggggaatgg
    16861 aattccttgg gaggctctct atctgtagaa taaagcccaa ctcctttatc cttcttaact
    16921 ttgggtacat ctgttgtgcc aaacatcatc tgactagatt cgcgagttgg ggctccaaga
    16981 aatatagcat tgaccaattg ttcacgaata tttgttgaaa ggctatcaga acggggttgt
    17041 tgcataacaa tgcagacccc catagataaa gaccttgctt tttgagcaac gatagcaacc
    17101 atattttcaa aatctttttt agctttagca tccatcgaag ctaataacga tgaatactcc
    17161 tctattacca acagtaaagg agggaaaccc aagtctatgc aaatactatc gaacaaatcg
    17221 cttttattgt aaatttccat ccgcctattc atctcgttaa tgtagtgacg cacgattcta
    17281 aaggcatcct cattagttgt tccataattt tctactggaa aaccagaaaa ttcgcataat
    17341 ttataaatat ctaaaaattt tggatcaatc acatatattt ttgcatgttt cccatttacg
    17401 gtctgagata tgaaacgagt caagtaataa tatgtaaaga atgatttacc actacctgag
    17461 gcccccgata taagcatgga aaagtttttt ctcaaatcga taacaaaatc atcatagatt
    17521 ttaattttca ggctatcatc ttcaagaggg agggaggtaa catgtaaacg ttcaggctgt
    17581 ctatatacaa aaacatattc aacatgagtc gcataaattt cttttgacga taggcttatt
    17641 cctagagcac tacttaggag aacgtctaaa ttgtccatct ctttcgtgaa ttcatcccca
    17701 gacttaaatg catagattac aattgaaaca tcattcaatt ggtaattaaa tttagctgaa
    17761 cgaagaatag cgccatcttt tgttgctgta aaaagaaagg aatttctgcg aagcataaat
    17821 agaaggtttt ctctgacaat taacagcggt tcaatctgct gagcccatcc cttgattttt
    17881 ctttcaatgt acaaaattat aattaattct ccaaataatg taatcatttg gagaaaccat
    17941 cccttaaaaa gaatactact agaaagggac aatatgctca aaaatcctat agtaaaccag
    18001 cgaatacttc tcaatttaga tgtatttaaa aaatagtgct cagataaaga agccgtttgt
    18061 ttaattttaa acatcttata cctatctaaa tttttttatg aaatccttga taataataag
    18121 taaaattctt aattttattt taaaaaaata gaaaacaaaa acaaataaaa acggggtagc
    18181 aaaaattgag caaagtaggg acatgccttc tccaacagga gtaaacacaa aaacagagca
    18241 tgccatcaat attatagtga tacaaaaggc taatctcatt tttacgatgt cattcatgag
    18301 tttcaccttt cttggttttt aaagcttcta ataatttagc aaacattatt ggaagttgca
    18361 ataaaattaa agtaactcct aataacaagg ctatcttcat agattgataa ggagagaagg
    18421 attttaggtc aacctgagct gaaaatcctc ctagcagaga cactatgaaa tataaactaa
    18481 taaaggcatt ttgacaaaaa ttcttaagca tatttttctc ctttttttaa atatctaata
    18541 aggaagaggg attaaccttc cttattagat atagctttta gggcgacgat gtattttacc
    18601 agatgagatg tcatccatga tagtcgttcc atctttataa tgagtacgat tagaagcata
    18661 tgctattttc ttcatcacat ccagtctcgc aatttttaat ccgagattag aattggaaag
    18721 catagcattt ttaatatctt ccaaatcttg agagttaata gaaagtaatg tcacatttcc
    18781 ttctaatcca tttattttcc tgatgaaaag gctaattttc tcagctgtct ctgttttcag
    18841 aaactctagt acatttctat tcatttctta cctcttattt tttctaatta ttgatttttt
    18901 ctagaaacaa aatcaattca acggtgaagc tctagctttc atcttcattc gtacctcctt
    18961 ttgtgtatat ctagctttac ataaacctat gatttagagt atagtttctc aattattctc
    19021 agaaactatg gagatgattt aattatcata caaatcaaag acctctcctt gctcatcttc
    19081 accgtaactc tcgagttcct cataaaagta ttccaaagca gtttgttctt ctggtgtcat
    19141 aaagaaactc ctttctaaac aatattagta aaaaatacgt tcttatttcc attctaaata
    19201 aagtagtgtt tttatcccgt aataggtttt atttttgcgg agcattacac tccgcattaa
    19261 tattgatagt attaagaatc attattactt aataaaaatt gcgctctcaa acatagacgt
    19321 atacaaaatg ccctatgtat acgtttcgct tggaagacat ctaagaaaaa attttgtagt
    19381 tcagacagta agattaatac atctttctct aacataagtg atgtctggta ctcccaatta
    19441 ttgctctgtt ttaacgatga aaaaggtata gctgtctctt ttaattttat ccaatctata
    19501 cgttcttttt taggaacgta ttctctaaca gcttgactaa caatactgga atagcttact
    19561 tcatagccta atttattgta aacatcatct ttaagcttat ctaatatctg cctggattca
    19621 gacgctaaac gcattctaac attctctttt cccatattat cacccctttc tgacgggatt
    19681 ctattatccc gtaaattaag tatataacaa tttaaaaaaa aaatcaagaa ttaagttgaa
    19741 aaaaattcca caatctaaaa atttatcatt ttatgacaaa catactatcg ttaaatgctg
    19801 atttaatcga aattttgagt tataaactcc tcagtttcga actcaaaatc agccgtgccc
    19861 ttgccgtgca ggataccctt cataaactta tataactact ttaatacctt aaaaagctaa
    19921 taaaataaga aatatctaag caatatacat tctaaaatac ctgtaggggg catataaata
    19981 caacaggaaa agtcttataa tatagggctt tttttgctta aataaaattt ttttcaaatt
    20041 ctttagaaaa ctagtttaaa tcataatcca acaccaattt tacaaatacc ataaactaag
    20101 cttatatacc caaaacacaa aaagtcctct ttccactatt ttagaaagag aactttttct
    20161 ttaggagaaa taacttaatt tttaatcaat tcgtgatagc tcagctggca ccgttaagta
    20221 ataagttgca gtcaagcttg cttcaccttg attgacaaaa atctcgatgg aatttttatc
    20281 tagaacaact tccaattctt tagcttcaat atctacatac cgtcgactag tgtcctgttc
    20341 ttcttcacct agaatttttt gaataagatg gctacgatca atgtaaactt gctgcacatt
    20401 actgtcataa ccaaattcaa gataatctgt atcatttcct aagtggtaat gacaatcttt
    20461 atctatttgg atttgatatt ggcctttttt aacagggaat tgtcttagtt tgccatcttc
    20521 caatcttaga attctaggta aagtcatggc acatgcccac ttgtgttctt ggtcatgggt
    20581 tggaagggta cgcccccatg tctgcatcca agcaatcagg atacgacgat tttgatcgtc
    20641 caacaatgtt tgaggcgcat agaagtcttg gccatgatca atttcttgaa ctgattctgg
    20701 gataaaacat ttttctcccc aatctacctt acccgtgaac aaaagcgatg agttgatgtt
    20761 atgatatgag tctccctcac gctgataacg catgggtgac ataataaggc aatctttccc
    20821 atctaactcg aagtaatctg ggcattccca cataaagcct tggtgttcta ccccttttaa
    20881 aaagatggat tcgaactgcc attctactag gttatcggac cctagtagaa cgatacagcc
    20941 cacattattc ttgtgtttgg cagctactac ggagtaatag cgtccatctt tttcaaagag
    21001 ttttggatca cggaaatcag cagcaatcaa ctcatctggc aagtctgatc cagttgcaac
    21061 tggattttgg gaaatctttt caaagtgaat cccgtcatct gaaaatgcca tattttgcac
    21121 ttggcggaca ccggtttttt cttcgatatg cccagtgtac atgagccaga ggcgatcatc
    21181 cttgacaatg gcagagcctg agaaacaacc atttcggtca taatcttgat caggagcaag
    21241 tgccactggc aagtgctccc aagtcaccaa gtccttactt ttagcatgtc cccagtgcat
    21301 aggcccccaa acactatcat atggatagaa ttgataaaag agatggtatt ctccacgaaa
    21361 atagacaaat ccatttggat cattgatcca accaatctca gctgaaaaat gttcttcagg
    21421 cttatattga gtattaacga gatgtttatt ttctgctata aaacgattgg ctttttctac
    21481 tgtaaatgtt ttatttatca taagtatatc ctattttatt ccagttccgg aaccacccag
    21541 accttgaaca atatatttct gagcgactac atagaccaaa atcaacggaa tcgttgaaat
    21601 agttaataca gccattacct gattgatata aactggttga gttgtattaa tagttgtaat
    21661 tgcaacctgc attgaaaatt tagaagaatc tgtcaaaacc atcaatggcc agatataatc
    21721 attccagctt gaaataaatg ataaaacacc aactgtagca actgcaggtt tagacattgg
    21781 aagcataatt ctaaagaaaa ttctaacgat acttgctccg tccatctttg cagactcaag
    21841 aatctcttct ggaattgcaa taaagaaatt cctaaataag taaatattaa atacactcgc
    21901 caatcctgga ataataactg caagacgatt attgaccaaa cctaaggaat tgataatggt
    21961 aaattgtgga attaacacag tttccactgg aataataagt aaagcaagta agaaaccaaa
    22021 taaaattttc ttacctgaaa aattgacttt tgcaaaagcg aaaccggcca gtgcattgac
    22081 aataatagaa ccaatagcaa acgttccagc ataaaaaatg ctgttcccta gatatgtaaa
    22141 aatactaaag cgctcaaata cttcctgata aggtttaaac caatcagcgg gatttaaact
    22201 tggtaagaag gcttgccaac tagtcaaatt tttataaaca tccgcttctg gcttcatagc
    22261 tgaaactacc atccaaacca taggaaaaag gaagagtcct gccaataaag ttaacagaat
    22321 atattctaag attgttgttg gtttaaatcg tttcatctta gtcatcctcc ttcagaacgc
    22381 gacgttgtac taagttaatc ataccaatta acgttgtaaa tactaaagcg attgagctag
    22441 aatatcctac aagacgatca gtaaaacctt gttgataaat atagtacacc atagtaattg
    22501 ttgagtttaa tggcccacct tgcgtcataa ccattggttg tacaatcagc ttaaaagcag
    22561 aaattaaagt tgttaacaat acaaacaggg ctgttggttt tagtaggggc atcgtgatat
    22621 aacgaaactg agcccatttt gagaatccat ctaattcagc agcttcatag acatcttgtg
    22681 gaatattctg cattcctcct aaaaataaaa gcatctgata acctgcaccc tgccaagcag
    22741 aaacaaaaac aattgcatac atagcttgtt taggactagt caagaaaggt tgagaagcaa
    22801 tacctacctt atttaaaata gcattcaata agccactatt tggatttaaa agataaagcc
    22861 aaagaataga aataactacc aaggacatga caactggagc aaaaaaagcc actttaaaaa
    22921 acatatttcc ttttcgcttt ttattgacaa tcaaagccat tcctaaggcc gctcccaatt
    22981 gaactggaat aatccaaaca acaaatttca aagtattcaa gaagctcttt aagaaaatcg
    23041 gatcttttgt taatcgaata aaattttcaa gtccaacaaa ttttctttca tctggtgtca
    23101 acaaataata atctgtaaaa gcgtaataaa tgaccattcc aactggaatt actaaaaaga
    23161 tacctaataa aattaaagct ggagctaaaa aactataacc taatatgtta tcctttattt
    23221 tttcacgtct ttttgtatcc acaacagttt ctttactcat atccatctcc tttaatcata
    23281 tatatcaaac taggctatta acaagaaagg ggaaggtagt tccaaacatt ttccttccac
    23341 cttctttttt acagattatt tgagtgactg atcaatagca gtttgcattt cttttgtacg
    23401 agtatccaag actttttgca catttggatt ttcttcataa tagctaatat cttgcattgc
    23461 ttgttgaaaa gctcgagaaa cctgaggata tgctacaaca actgggcgag catgagctgt
    23521 ttttgaattt tgttccatca agaaacgcat tggttcggat actttatctt tgatattttc
    23581 aattgtagat ttacgaattg gaaggaccga attccccaag ctaataattt cacttgattc
    23641 tgtatttgta gcaaatttaa caaattcaga tgcggcctct tttttatcag attttgtcgt
    23701 cacagctaat tgccaactac ctgaagggga tactaatttt ttcgtcttac tagatactgg
    23761 gtaaggaagg ataccaaaat caatatcttt gtagtttgta ttcatgtcag caatcgtcca
    23821 agaaccactc aaaagcattg gatactctcc tgtttcaaaa cctttttcga ctggactaac
    23881 agttgtatag ccttctttta caagattttg aatgaattga actgcttcta cactctcttt
    23941 ggagttgaag tatccttctg cctttgttcc atcttcgttt acgacagaac cattatttga
    24001 ccaaatcaac ggcatataag catatggcaa catttcatcg tttgaattaa tacgaaaatc
    24061 aattgctggt ttgttataat gatctttcaa ttttttagca atcgtattaa attcgtccca
    24121 tgtccatggc ttttctagcg ttggaagctc tgattccgca attccggcct ctttgaacat
    24181 ttttttattg taataaacac caacattcga ttcagaaaat ccaaatgcat agaatttccc
    24241 attataagtt ccctgttgtt taatactgtc caaaacatca tccatattat tatcttttag
    24301 ataatcatct agcggagtaa ttactttaga ttttgcataa gctgccgtat ttggtccatc
    24361 aagggtaata acatctggaa gactattagt cgtaatagca gcattaacct tgtcttcata
    24421 tcctccacca ctaccactac gaggaatata ctcagttaca accttgtact tactttttcc
    24481 tgattgatta aagttatcaa caactttttg ccaagctttt ccttcatttg tttcgtcaga
    24541 gaattgtacc cagactttaa tatcatttga agaactcgct tgttttgagt tagttcctcc
    24601 actacaacct gcaagtatag ctaacgatag ccccgcaaca acagataaca ttgtaccttt
    24661 acgcatcatt ttcctccttt ttgaaacgtt tcatttttta tttatttttt aaacaagctt
    24721 ctagcctatt tttgaaacgt ttcatttgtt gattatatta taacagcgct ttcattaatt
    24781 gtcaagcgct ttttaaattt tttttgttgt tttatctccc caaaaatatg tgggaattac
    24841 gattcgtttt tctagaatct cttctcgctt aatcattttc agtatcattg caacagattc
    24901 ttttgccatc ataccaatat cttgaactat tgtagacact agataagatt gatcgacaga
    24961 agtttttaaa ccatcaaacc caattatctg gtaatcatca ggtgctattt tccccagttc
    25021 tttcataaca ctaataactc tcagagccat catatcattt attgtaaata taccatcaat
    25081 ttctttatga gattgcaaaa aaattcttat ctgatcttca ggatttatta aaggttcttt
    25141 catttcaaaa ttatagacat ctactcctaa ttccttagct ttttctctga atcctttact
    25201 tctgagtata gtctcattct catacatatt aattgaacca ataaaagcaa ggtgcttatt
    25261 atcccttcta accagttcct ctgctgcagt ttgtcctcct ttgtaatttg ctgaagtaac
    25321 atagcataca ttttccgaaa aatgtctatc tatacttaca aacggtaaat cagataaaat
    25381 atatttgtca atatcagaat atgtaatacc aataattcca tccactttat tttgtttcaa
    25441 catttcgata tattcgattt cattttgtga atcgctatca gcgttacaaa taaataattt
    25501 ataattatat tttaataact ctttctcaac atgataggca aactccgaaa aaaatggatg
    25561 ccatatgctc ggaataatca aagcgatagt ttctgttcta ttctttttca tacctcgagc
    25621 ataattatca gggatatagt ccaatgtttt tatagccctt tgaactttcc ttaaagttac
    25681 ttctttaatg cctttttctt tattaattac acgtgaaaca gttccaacac taactcctgc
    25741 ttctaaagca acatctttca tggtaattga ttttctttgt tctaccatat tatcacctcc
    25801 tttcaatata tagtatcatg caaatgcttt ttaagcaact atttctcaat catttttggc
    25861 cagatcattt atcccatcat gaataaaatc actccaatta gcttttgaaa atacttcaat
    25921 tttcatgtgt aaacatctac ataaaacagg aaaagccttg gttttatggc ttttttcgta
    25981 tcttctacaa aaaaagcaag agttttagag ggctataaat ctagatgtac attttgctta
    26041 aatgattgaa ggtcttttct taacaaaaac accccaaaaa ttagattttt tctgtctaac
    26101 ttttgaggta cagttcaaac gcgaaatagc gtttttttgt tatttttggt tactcatcta
    26161 atcgaataaa catcatggca tttaacaagt atatgagtga gaccgtgttt atattatttg
    26221 aatagatgag tctcttattt tcaataggag gaataataaa attagaaata atgatatcat
    26281 aaggtgaatc ttctaaagat tcctttgata attctaattc agtccaaact tccagttcaa
    26341 aattattgct acagtaataa gaaagtgtct ctgcaacgaa ttttgcatga tactgatcaa
    26401 aattactcat aactaaaacc tttagtttag gctgattttg tagcaaatta atcaccaaat
    26461 gtttggtatg agtgatgaag gtataagata gatgatttac catcattgaa ctagaacaaa
    26521 cctcaagagt ctctaaatag tgagaaagct ctttttttat atctgaaaca aattttggaa
    26581 aaatattttg aaagttcctg attgtattcc ctttttgatc aaataaaata aactcagtaa
    26641 acaactcttg acgatacaga tgtgcggtat tatgcagatg ccaaatcaga ttatccttat
    26701 tctccatttc aatctgatac ttgactgaaa tctgatcaat aaaatcactc aatagatggt
    26761 aagatttttc aacataacta tcctttttta cgcatttcat aaagagactt tcatctatga
    26821 aaaacatttt ttgaaagtaa gacacaaata attggcaaac aacttcttca tctaaagaga
    26881 tattgtattc tgattcaaaa ctctgagcaa caccttctat tccttctgcc tgcattaaaa
    26941 aatccaaact ttggtcgtta aaagaatctt tatctacttc cataaaatga ccaaacttta
    27001 ttctatatag gttcgtaact aggagcaact ttagcattct atgcgttgac aaattcattg
    27061 gaaagcttgt ttccttataa accaattcta acaattgaga tagtggctct gatgaaaaat
    27121 tttcaaatgg ccattctagg aaataatatt tttctgaaaa atattgtgca aaaaagtaac
    27181 gaatgtctct ctcatttcca atgatttgaa caggggtcag actgacttca aattgaaatt
    27241 gccttttaat cactttattg atttggctaa taatacgata gagcgaagat gaactgatat
    27301 aaaattcttt acaaatactc tcagcttgac aaccttcatt aaagaagatg aattctaaaa
    27361 tcgaaaaatg agttgaatgt ttaaagaaat gatggtaaac catttcaata tcactatcat
    27421 cggtattaat aatgcgtata ccattagtag aagaatgaaa aatcaagtca ggaaaagcag
    27481 atttaacatg ggatagatca tctttgactg cacgttctgt acaatttaat aactctgcta
    27541 gttcagaacg atgaaaccaa cgtttatgtt caaataataa ttctaataat tctaattgcc
    27601 tatgactttt tttagataat aaatctctca tgaatatctt tctctcttta taaattatcg
    27661 gattaaacct cttgcaatta taccacaaag aataggtata gcatgatata acgacttttc
    27721 ctaaaatctt ttatttcgta taataacact acggagacaa tatataaaca attttcttat
    27781 tttaccgtct attgagggcg tgaatacaga atcaaattca agtctaaaga ttatattttt
    27841 aattttaaaa attatataat agcaacaatt aaagaatttg attttttaaa attatataat
    27901 aataacaatc gaaataattg acttttctat attaaagtta tataatagta ttaatcaaag
    27961 aaattgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt
    28021 gctggaggtc ttattggtct cttggcaggt aaaatcacta aaaaagtagt tctatgggaa
    28081 tcatcgcaaa tgtattcgct ggtttagtcg gggcatatgc aggacaatct cttttaggta
    28141 gttggggtcc atcaatcgct ggaatggctt tgctcccatc tattataggt gcagcgattg
    28201 tgattactgt agtgtcattc tttacaggta gaaagtaaac ttttcgccag taaagttagc
    28261 aaactatttt taaatcaatg acgggaaaaa tagtttaaat gttaaatcga aaggattgta
    28321 tatgtcaaaa gcaaagaaaa tatgtttcat tattttctgt attttaatct tgacaatttt
    28381 ccttcctgtt ttgatagatt atcatcaagt tagtgatcta ggtattcatc tacttagctg
    28441 gagacagaac tccgtagttg aattctatct tgctagatat gtcttttggg ggacagtggt
    28501 tctatcaact ttagttttat tatccatttt agttgtgatg ttttatccta aacgttactt
    28561 ggaaatccaa cttgaaacta aaaacgatac attaaaatta aagaattcgg caatcgaagg
    28621 ttttgttaga agtttggtga gtgatcatag attgatcaag aacccaactg ttcatgtaaa
    28681 tttacgaaaa aataaatgtt tcgttcatgt agaaggtaaa attcttcctt cagacaacat
    28741 cgctgacaga tgccaaataa ttcaaaatga aataactaat ggattgaagc agttttttgg
    28801 tattgagcgt caagtaaaac ttgaagttgc agtaaaaaat taccaaccaa aacctcaaaa
    28861 caaaaagact gttagtcgtg tgaagtaagg aagtaaaaaa tggaatggct taaacaatat
    28921 cgatatccaa ttatcgctgg tctcataggc gtatttctgg cttgtttgat tgtctccttt
    28981 ggcttcttca aaacaatatt tgtattgatt ttaggagcac tgggagttgc agctggatta
    29041 tatatcgaaa aaaactatat agataaataa aaaaataaaa attactaatt taattaaagg
    29101 agtttcatat gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa gatgctactg
    29161 ttgtagctca cgaaatcaaa ggggaactta cttacgaaga taaagttatc caaaaaatca
    29221 ttggtctttc actagaaaac gtttcaggtc ttttgggaat cgatggtggt ttcttctcaa
    29281 atcttaaaga aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac gtagaagttg
    29341 gtaaaacaca agttgcagtt gacttaaacg ttattgttga gtaccaaaaa aatgttccag
    29401 ctttatattc agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg actgacttgg
    29461 aaattgttga aatcaacgta aacgttgtcg acatcaaaac taaagaacag catgaagcag
    29521 actcagtaag ccttcaagat cgcgtatctg acgttgctga atcaacagga gaattcactt
    29581 cagaacaatt cgaaaaagct aaatctggtc ttggatctgg tttctcaact gttcaagaaa
    29641 aagttagcga aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta tctcacgaaa
    29701 acactcgtgt aaactaagat aaaataaata taacaggaga aattatcatg tcagtagaag
    29761 aaaaattaaa tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc atcggtgatg
    29821 aaaaaatgga aaaagaagga gcagctgaaa aagttgtttc taaagtaaaa gaagtcgccg
    29881 aagatgccaa agacgctgta gaaggtgctg tagaaggtgt taaaaacatg ttgagtggcg
    29941 acgataaata aggttaaaag ttactttatc tttttagtaa cattagtcaa aagagtctga
    30001 gtcaagatga ttctcagaaa acaaaaagct agggattccc aattgcggaa ctctagcttt
    30061 ttaattttgc ctctttctct tattatattt cagcaggttg ttggccatga gtacgaatcc
    30121 catgtcaatt ctcacttgac gcttccctct cagatgacat ctcttataac ccaaacaaac
    30181 ctttatctgc ccaaagacag atttcatatc aatcttacgt ttagcgaaaa tttgtctacc
    30241 cttggaagat aaaagtgcct gatattcttt agtttttaaa cactggtaac gttcattcat
    30301 atacagtccc ttttgagggg ctgattcagg ttcataatcg cagtcaacat tgatttcaag
    30361 gctgtttgct ttctatctac cctttttcat gatatatttt cgtacaggtt cttcaatagt
    30421 ttctatagta aaatgaacca aaaatagtac acgttgcggt ataatgttct tgtggcatat
    30481 tcattagatt ttcgtaaaca gttctctcct atttgtgagc gaacaggtat agtcaattga
    30541 ttgtcagttt gaagaccaag agtcgtaagg caaaggatat ggcagaatcc atccaagggt
    30601 ggctggcaca atttctagtg aacctcttta aatctattac gtttgactgt ggaaaagagt
    30661 tttcaaaatg gaaagatatt tccaatcatc acgatagtga gagctttttt gcgaatctag
    30721 gatgttcaag acagcggtgt ctcaacgaac attcaaatag acttttaagg tgtcatgact
    30781 tgccaaagca gacagatttc aacgaagtca gtcaagaatt ttaggctgga caaaaactca
    30841 atttcaggag aaaatgaaat aaatattccc acaataaaac gcatagtatc aaagtttttc
    30901 caacacctga tattatgcat tttttaattt taaagacttt ttcccagcct caagaaatca
    30961 catcccacct aaatcctttg gataccaaat acctcatcag atattttctg agttggtaag
    31021 actgtctagc ttaatttgac aaatggaggg cgttaaatcc agtttttaca ggaagtctat
    31081 ttaaatattt tcacactgac cttacaaaat atgaaataaa ttatataaag tcattcccat
    31141 gacaaagaat aacatccctt taaaacagta tcttacccaa ttttcaggta agacttttga
    31201 tacaaccgtc cccaataccc ctccgcatat agaagcaaga aaaataactg gaacaggtgc
    31261 taaatgatac tgatttaaac ctgtaaccac aacaattgta gccaaatgag acagctggga
    31321 aaagaaaata attgcaagag aatacatcgt tgcttctttt atcgaaatag aaaaaaacac
    31381 catcaataaa gatacattga gcggaccacc accaatccct aaaaaactag aaattaagcc
    31441 caatagaaga ccacaaatca tatagaatcc tatcttatca aattgataag tttttttaaa
    31501 atctgttaaa acaaatgata caagtagcat cacaaatagt aaaatcattt gaacaatgga
    31561 tactagccgt actgataatt gagataacaa aacttgaaaa ataagatgac ctagaaatcc
    31621 tcctaaaaca gaagaaaagg ataccgtaaa aatcaatctc cattttatct ctttactttg
    31681 tgaaaatctt ttgattgttg aaataatcgc cattataaaa acagagaaac tagaataaaa
    31741 agcaatatca ctgaccgaat ggtacccaaa actatccata agtggcttaa ttataattcc
    31801 accacccatg cctgatatag aacccaatcc actagctatt ataatgatta gaaaataaat
    31861 aatctcaacc actttttaag tccatcctat tctttcaaac atctaaaacc tagatggcta
    31921 ctagtggaag tagaaataca tccatttcta gcagctactc ggtatcgatt acaataagaa
    31981 caatgacata aaaaggaacc tcctctgata gcatactcct catcttgtat cccatatttt
    32041 ggcaactcat agtcatcttc attaaaacta gctaataagg tatatctcgg attgcgacac
    32101 cattcccaaa cattcccaat catttgatat agaccaaaat cattaggtaa aaattcatag
    32161 acaggagccg ttcctataaa tccatctgag acagtatttt cataaggaaa tcttccttgc
    32221 caagtattgg catgataata tccaccttct aaaagagtat ctccccaagg atattcagat
    32281 tgtcttcctc ctctagcagc atattcccac tgtgcttcag tcggtaaaga cattccgctc
    32341 caattacaga aagccaatgc atcttctaaa gcaacatgaa ctactggatg gtcttctaaa
    32401 cccactagat tactattctc accgtaggga tgtttccagc aagcgttcgg aacttgtaac
    32461 caccacggag ccccagcagg atgaggatat ccctcccttt ctgcctctgg cattaatatg
    32521 gtttcctgga atcacattcc caatagttac catcagattt ttaccatcaa atgctagatt
    32581 atcaattcct tgtaagatct gatctaattt cacatctcct acatttgcca aagggaaact
    32641 ctttgtaact gtacttccaa tcgaaatgac aaacgaagca agtgatacca aaccagcaga
    32701 aactgtatca accttgtaaa tcttagcgat attcactccc aagcaataga tgaacaacaa
    32761 ggaaacaatt ggtatacttc ccttgaatac caaattattg atgtcaacaa gccactgaaa
    32821 ggttttcgta atactttcta ggtgaaattg ttgtggtaaa tccactagaa aagcatttaa
    32881 taacaaagca atggaacctg tcataataac aggcatagtc cccacaaatg aatcacgtaa
    32941 agcaactaaa aacttctgat tcccaacttt cattgcaatt ggagaaagtt tttcttgcat
    33001 tttatccaac attgtattca taccaaactc ctaatttttt tcaaatcgtt cgtatacttc
    33061 taccacttct ttcgccaagt caacaaaagt cagactcgtc attaaatgat cttgcgcgtg
    33121 taccatcaag aggctaattg atgtttttcc cccctgtgcc tctcttgtca acatttctgt
    33181 ttgagcctta tgcgcctgta ataaggcagc attcgcatca gctaatcttc gattggcttt
    33241 agagaaatcc ctttttttgc tgcctgaata gcctccatag cattactttt ggcatctcca
    33301 ccatacataa ttagccccat tacagattct aaattacttt catccataac attctcctca
    33361 atcttagtct aataaacttt cagctaaatc taaaacttta gaaccattca tcattccgta
    33421 atctgtcatc ggaataaccg ccacaggaat ctgtctatct tttagttttt cttgaaagtc
    33481 ccctagtaaa tagcgaactt gggggcctaa aagcaacaca tttacttctt ttgttgctac
    33541 aatttcttct gcttcaggag ctggaactgc aaaaatttct gcatccaacc ccttatcttc
    33601 tgctgccttt tgcatctttg tcactagcag acttgtactc atacctgctg cacatgctaa
    33661 cataattgtt actttagcca tagcttactc ctttatcacc tatttactga cctagccaag
    33721 cgagatactg tatgtctcac tcctctaatt gccttgctca atgcataaat attatcgact
    33781 gttgccagtc ccccatatcc tgcgtcacct atatgttgaa tatcaactcc acaaatttta
    33841 tttctaagtg caatttcctt aatagtatct gtatcagatg tttcttggct agtaccaata
    33901 gcactcaata caagaccacc cttactatga acgagatcaa cgacttcacg caactcttgg
    33961 tcatgaaagg ctggaacggt tccaactgct ggaacaagta tcacatccgc acctgcttcc
    34021 aacaattgct ctgcgacaga aagctctgcc acaggctcat tcactcctgc cccgtgcatc
    34081 ttacctgcaa taatcaaacc agaaaagttt tccttagcag tttgaacagc cttaatgatt
    34141 tctcgattgc taactcctgt tccaggattt ccagtcaaac agacaaagtc aaaacctaac
    34201 tcctctattc gattcaatgt ttcaacacta gcaacacgac ctgcaacaat ttcctgtgtt
    34261 tcctctagca tctttgcaga tgggtcaatc ggttccaaat ttacaccaat tggacaagca
    34321 acaagctggt gtaagcgatg aataacatca tcacctgaac tatccaaagc ataaatttta
    34381 ggctcaaaaa catcaacaca attcaataaa atcatatcag ctccaaaagc tctagcaatt
    34441 tcagaattag ttatatcccc aacaaatgtc tcacgagtaa ctacattttc agatagaaca
    34501 actctacctt cacttgctaa aatactttgt tttaattctt cagcattcat ctgtaatatc
    34561 tcagatggat ttgcactaat taaacgtttc attctcaatc ctccaataac ttaaaccgtt
    34621 tttcaatagc tattacaaat ggtaaataaa gtaagacatc caaaacaatc aatacaattt
    34681 gtaataatga accagaaata cttcctgtag caagtaaacc acttataaca ggtggcatag
    34741 tccaacctga atctgtataa gttagcggaa ccaaacctga tgccattgct ccccacgcta
    34801 ctagtaagtt aaacattgga gctaagataa atggaactag taacaaaatg ttcaaaacaa
    34861 taggaacgcc aaacattgct ggttcattta tattaaataa tccaggtata acagttatag
    34921 gtgccaaagt ttttagttgt ttactagctt tcttcttatg tgctaaatat cctaacgcaa
    34981 gaactaaacc tatggttgca ccaccgccac ctatgaatac aaaattatcc ataaatacgc
    35041 tagtaaaaat atttggaaga gtttctccag tttgataagc tactttattt gcatctagat
    35101 tagctaacca taacggtttc ataactgcat taacaacatt tcctccgtgt aatcctacag
    35161 accaaaaact actattaagg aaaattaaga taagaagacc aataacatta tttcctaaaa
    35221 gtccaagtgg acctcccaaa gctacttgag caatttcatg aagattaggt aaacttaatt
    35281 tatctaaagt tgcataaaca atcaaccaac caacaatagt cactgcacca ggaataatgg
    35341 cactaaagct tttagatact gctggtggaa caccatccgg catttttatc tgtatattat
    35401 gatttataaa ccattggtag atatagccat taatcaatcc taaaatcata gcaacaaata
    35461 aaccttttga tgccatgaaa gcggtaggca ttcctgctcc agcctctcct ctaatgaaag
    35521 gcgtaactgt aataaaagat gataatgaca caataccagc aggaatacca tcaactttgt
    35581 attgtctagc cataaagtaa gcgataccaa aactagctac aagaccaagt aatccaaaac
    35641 tactatcaac acccttccat aaatagtcag caacaccaat atcacccaga tatttttccc
    35701 atcctggaat aggaaaactt gcaataacca ttagtaatga acctatcaac aataaaggta
    35761 tagttagagt aataccgtct cgaatagaaa ccaacgcttt attattaccc aatctagccg
    35821 ctactggaag taacttttct tgtaataatt tttccataac cttttcctct taaatatatt
    35881 ttattatcaa aagttatcaa ttatctttat tttttgacgt caagttgtta tttgattatc
    35941 ttttgttatt tatattttac aactataaga aagccttgtc aaggatttgt gtaaacattt
    36001 gattattttt tattatcaat atataataaa aagagaccag taagtaatga aaagcttaaa
    36061 aagattaaca tctacattta tccatagcaa aaaatcatag gattttaaga atctgacaac
    36121 taggaagaaa actaatatga ccttatctaa gaaacaatta caactaagag ctaaaatact
    36181 ggaaacagtt tacacattag gtcccatttc acgaattgaa atcgctacta aaacaggaat
    36241 aacacctgca acaactagca gtatcacaaa tgatctgatt aaagaaaata ttctcttaga
    36301 acttggtgaa gacgaacacg atacaagcgt agggagaaaa aaaatcctgc tagacattca
    36361 agctaaacga ttttactaca ttggttgtga actatctgaa aaacatttca cttttgcttt
    36421 aggagataat ctaggaaata tcttaaagga agagaaagaa attgttacta aacagttaat
    36481 ccaagaaaag ggaaatcaac tcattaacca gacactaaag caattcttaa ataactgctc
    36541 tgattatgaa atagaagcaa ttggaatcgc gctacctggt cgctatttag atgattataa
    36601 aataacaaca aacaatcctt tatggcaaca tatcgactta gaaatgattc aatcccactt
    36661 tgataagcct ttgtttttct ctaacaatgt taactgtatg gctataggta aacgcctatt
    36721 tagtcgccaa cagaatgacc ctaattttgc ttattttcat tttgctagag gtatgcactg
    36781 ttcttatatc tacgacggaa acatttatgg gaaaggaaat ctaatgattg gagaaatagg
    36841 gcatacagtt gtttcatctg agggagaaga atgtagctgc gggcgcaagg gatgcttaca
    36901 aacttttgct ggagaatctt ggttaatcaa aaaatcaaaa attctatatc atcaatctcc
    36961 ctattctcta cttcctagtt tagtcaaaaa tgccgatgat attgatatcc aagttatttt
    37021 aaccgcttat caattaggtg acactggaat aatcacactt atccatcaag ccttactcta
    37081 tcttagtcaa accattttaa atatcagtat gatgattgac tctcaaaaaa tctacctaca
    37141 tagtccttta ctaacaaatc aacacatcat ccaaaaacta tatagtgaaa tgaactacaa
    37201 acccaaactg ctctataatc gcctacctga agtcattatt gaaccttaca atgattttac
    37261 agccgcacac tcagctattg cactttgttt gtatcataca attctacatt cttaattttc
    37321 tattacattt aataaatagg ctaaaaagag ggagtatatc ataaaaagcg aataaacctg
    37381 ttttctgaaa atcagaattc tgttttgttc gctttcttta tatttggaat tatactccta
    37441 tctccccttt ttcataatgt atagtgaaat gaaataataa caggacgaat cgatc
//
LOCUS       DAVBDG010000016        35689 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBDG010000016 DAVBDG010000000
VERSION     DAVBDG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.016"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(65..1051)
                     /locus_tag="VQ526_000746"
     CDS             complement(65..1051)
                     /locus_tag="VQ526_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2554492.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1061..1861)
                     /locus_tag="VQ526_000747"
     CDS             complement(1061..1861)
                     /locus_tag="VQ526_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2554493.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2122..2964)
                     /locus_tag="VQ526_000748"
     CDS             complement(2122..2964)
                     /locus_tag="VQ526_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2554494.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(2966..4258)
                     /locus_tag="VQ526_000749"
     CDS             complement(2966..4258)
                     /locus_tag="VQ526_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2554495.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4369..5640)
                     /locus_tag="VQ526_000750"
     CDS             complement(4369..5640)
                     /locus_tag="VQ526_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2554496.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6190..7707
                     /gene="malQ"
                     /locus_tag="VQ526_000751"
     CDS             6190..7707
                     /gene="malQ"
                     /locus_tag="VQ526_000751"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2554497.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7733..9991
                     /gene="glgP"
                     /locus_tag="VQ526_000752"
     CDS             7733..9991
                     /gene="glgP"
                     /locus_tag="VQ526_000752"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HET2554498.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11036..11608)
                     /locus_tag="VQ526_000753"
     CDS             complement(11036..11608)
                     /locus_tag="VQ526_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554499.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGNWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(12231..13079)
                     /locus_tag="VQ526_000754"
     CDS             complement(12231..13079)
                     /locus_tag="VQ526_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2554500.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13231..13509
                     /locus_tag="VQ526_000755"
     CDS             13231..13509
                     /locus_tag="VQ526_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554501.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13511..15574
                     /locus_tag="VQ526_000756"
     CDS             13511..15574
                     /locus_tag="VQ526_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2554502.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRICVTD
                     NQMNSRLLQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLTRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15652..16908)
                     /locus_tag="VQ526_000757"
     CDS             complement(15652..16908)
                     /locus_tag="VQ526_000757"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2554503.1"
                     /translation="MHIFDELKDRGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17050..19515
                     /locus_tag="VQ526_000758"
     CDS             17050..19515
                     /locus_tag="VQ526_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2554504.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19580..20491
                     /locus_tag="VQ526_000759"
     CDS             19580..20491
                     /locus_tag="VQ526_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2554505.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20721..21419
                     /gene="dapD"
                     /locus_tag="VQ526_000760"
     CDS             20721..21419
                     /gene="dapD"
                     /locus_tag="VQ526_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2554506.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21487..22617
                     /locus_tag="VQ526_000761"
     CDS             21487..22617
                     /locus_tag="VQ526_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2554507.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALACELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22629..23168
                     /locus_tag="VQ526_000762"
     CDS             22629..23168
                     /locus_tag="VQ526_000762"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2554508.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLMPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23152..23829
                     /locus_tag="VQ526_000763"
     CDS             23152..23829
                     /locus_tag="VQ526_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2554509.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGL"
     gene            complement(24130..25062)
                     /locus_tag="VQ526_000764"
     CDS             complement(24130..25062)
                     /locus_tag="VQ526_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554510.1"
                     /translation="MENKFVEFIENNKKVIISSVAVGVVLILGFGWYSYNQQQAEQQA
                     KIVQLEKDSKSDKEQVDKLFESFDASSDESISKLKELSETSLKTDAGKDYLNNKVKES
                     SKAIVDFHLQKGLAYDVKDSDEKFKDKATLETNVKEITKQIDFIKKVDETFKQENLEE
                     TLKSLNDLVDKYQKQIELLKKEEEKAAEKAKESSSQSNSSGSASNESYNGSSNSNVDY
                     SSSEQTNGYSNNYGGQDYSGSGNSSTNGGSSEQYSSRNSNIGANNGYTEYRSIDASGH
                     VRDTYHNRSNGDVFDENGNYLGNLGGGIAEPPQR"
     gene            complement(25480..26379)
                     /gene="galU"
                     /locus_tag="VQ526_000765"
     CDS             complement(25480..26379)
                     /gene="galU"
                     /locus_tag="VQ526_000765"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2554511.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(26401..27417)
                     /locus_tag="VQ526_000766"
     CDS             complement(26401..27417)
                     /locus_tag="VQ526_000766"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2554512.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            27629..28009
                     /locus_tag="VQ526_000767"
     CDS             27629..28009
                     /locus_tag="VQ526_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2554513.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGGEDYKLMPSYIELDKECLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(28183..28608)
                     /locus_tag="VQ526_000768"
     CDS             complement(28183..28608)
                     /locus_tag="VQ526_000768"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2554514.1"
                     /translation="MTNLSSVDSEELFQFYREQRNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKVLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYKARGQGKTCLTE"
     gene            complement(28684..29334)
                     /gene="phoU"
                     /locus_tag="VQ526_000769"
     CDS             complement(28684..29334)
                     /gene="phoU"
                     /locus_tag="VQ526_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2554515.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(29349..30101)
                     /gene="pstB"
                     /locus_tag="VQ526_000770"
     CDS             complement(29349..30101)
                     /gene="pstB"
                     /locus_tag="VQ526_000770"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2554516.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(30103..30918)
                     /gene="pstA"
                     /locus_tag="VQ526_000771"
     CDS             complement(30103..30918)
                     /gene="pstA"
                     /locus_tag="VQ526_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2554517.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(30911..31774)
                     /gene="pstC"
                     /locus_tag="VQ526_000772"
     CDS             complement(30911..31774)
                     /gene="pstC"
                     /locus_tag="VQ526_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2554518.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(31892..32767)
                     /locus_tag="VQ526_000773"
     CDS             complement(31892..32767)
                     /locus_tag="VQ526_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2554519.1"
                     /translation="MKFKKILTLAAIGLSGFGLVACGNQSSTSKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(32934..34265)
                     /locus_tag="VQ526_000774"
     CDS             complement(32934..34265)
                     /locus_tag="VQ526_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2554520.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(34266..34973)
                     /locus_tag="VQ526_000775"
     CDS             complement(34266..34973)
                     /locus_tag="VQ526_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2554521.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGKQEGDDSDNIADNSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(35057..35542)
                     /locus_tag="VQ526_000776"
     CDS             complement(35057..35542)
                     /locus_tag="VQ526_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2554522.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            35528..35689
                     /locus_tag="VQ526_000777"
                     /pseudo
     CDS             35528..35689
                     /locus_tag="VQ526_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        10543 a         6701 c         7535 g        10910 t
ORIGIN      
        1 tatcaagttt tttcaatacc tgatagtatg cgtttttgct tttaacgact ttttgtccag
       61 tctcttattt ttcgataatt ttgtgggcga tcaattgacg gtaacaaatt tgtttattgt
      121 ttttattatc attaataatc tggagaatag tttcaaggga aacacgacca agctctaaac
      181 tattgatatc gacataggct gccaagttga gcttgggatt aaccgagtca aagctgagaa
      241 cagggacatc cagctggtgt ttggcaatat agttacaaac accttcagct aggaggctat
      301 cggttgtgat gatagcatca atttgtggat cgtgcttgaa taatcgcttg ctaaatttat
      361 agcccttttc ttccagaaac tcgtcggcaa agtagatgcg attgttgtca gtggtaagtt
      421 tgtaatgttt aagcgcctgt tcatagcctg ttaaacggtc tttggtcacg aagagctttt
      481 tacttcctcc gataaaggca atgcgtttgc agcctttttt gatgaaatat tcagtcgcat
      541 caaaaccagc ttgaacattg tcgttgtcga caagtgggat gaaaggagat agagatttac
      601 ctaagataag gaaggggaac tgttcttctg cgacgagttt tacgagaggg tcttcttctt
      661 gggcatagag aaagattagc ccatctacac gcttgccgta gaccatttgt gaaatagcgt
      721 tgagacgctc cttctcatct ttccctgttg ctatctgaat ggcatagtgg ttttcagatg
      781 cgacttgaga gatgccacgt agaaccgatg gaaagaaagg attctggtag aaggcgtctg
      841 agtcatcagg aagaactaat ccgataacct gagtatagct gcttaccaag ctacgagcgt
      901 tgaggtttgg gtggtagttg agttccttca tagctttgcg aacgcgtttt tttgtttcgt
      961 cgctaatggt tgatttattt tgaataacac gggttacggt tgaaggcgaa acaccagcag
     1021 ccttggccac gtctttaatc gtaacgggca taaaaatctc ctatttatgg taatttggat
     1081 cacggaaatg tgttttataa aagatagtga ccagatacag aacaaaaaga atattttgta
     1141 cagtaatcag ggttgtcata ttttggccaa ataatcccaa aataagtgta atcagagtcg
     1201 gcaatcctaa acagttcaag ataaagtgat agcactcttt aaaggttcta aatgaaaaga
     1261 ggcgtgattt cttagtgata taaaggagaa gactagctcc tagagagacg ataaagaaat
     1321 tcaaaccaaa gaggaagctc gcaccgagaa ctaggaagag gctgatatag acacgatttt
     1381 gttggtacca gtctttagaa attgcttggg tcaagctgtc tttgcttttg aaactctcag
     1441 tctgaatggc tcggtaagag atgcgggtca gttccttgct ttccttgctg atgactagct
     1501 catttgtatc gaaatgcagt tgcaagttct taggtaattc cttgatttga cttggaccaa
     1561 tcacaacaga agaggcttga ctaactgttc cagtataagt taatgtgcca tcgacaattg
     1621 tagcatgttc agagagatcc tggacaacct catttgtcag aggttcatag acattatcga
     1681 taaaagtttc tagcggatag gtctcctggg agctgttttg gatagcaatg ggtaccatag
     1741 acaagctgat aaggaagata ctggtaaaga gaagttgaaa ccagttgagc ccgaaacgtt
     1801 tggacagggg cttacgaaac cccaaaatac ttgaaaaaaa ggaaaatgga tatggaagca
     1861 taggtatctt tctaaaaggt gttttctata taagtattat tatatagaga aatgcgcttt
     1921 atttcaagtc taggagaaaa attgcttgct acaaggatat tatttacagt gacaatcgct
     1981 aatagtcaat gaaaatcaaa gtgcaaacca ggaagctagc tgcaggttgc tcaaagcaca
     2041 actttgaggt tggagataaa gctgacgtgg tttgaagaga ttttcgaaga gtataaaatg
     2101 aaaaagggtg gcggggataa attatccctt gtcgccacca cttgtaagtc ctgaaacaaa
     2161 gttcttttgt aggaagaaga agagaataca gattggaagg gcgatgagga tagcacctgc
     2221 tgagaagtag gcaatcttca agtttttcgc attgttaacg aaggtttgga gacctacggc
     2281 aacagtaaag tattctttct cacgaagcaa gaaactagag aggatgtagt ccccgaaagg
     2341 tcccatgaag gcccagagag cttgtacggc aaccattggg cgaacaagtg gaagaacaat
     2401 ttgccagaag cggcggaagt gtcctgcacc gtctagtttt gcagattcgt ctaaagacat
     2461 tggcactgta tcgaagtagc ctttcatgag ccaagcattc atcgggatac caccaccaac
     2521 gtagaggaag atgaggaacc agttgtggtt aagggcgttc aacataagcg ccataacgaa
     2581 gaaggctgtc aaagcggcca tagttggcac catttggatg atcaagaaga agaccaaact
     2641 ttgtttacga gccaagaagt tgtaacggct gtaagcataa ccagcaagta cgatgatact
     2701 tgtttgaaca gccatggtaa ttaaggcgat aatcaaagtg ttgaggtacc aagtaccgta
     2761 caaggtttca gtgaagaggc ctttaaagtt atcaaaattg aggtcgatat tagtatctag
     2821 tttaaaggct gagacgttac ctgctttaaa ggctgacata atggtaatca acagtggata
     2881 gataattaca attgatagac caatcaggta aaggtaagta aggctttgag tcagtctacg
     2941 tttgagttta attgagttat tcatcttaga cgtcctccat atcaaatgcg tgtagtttct
     3001 tgaatgcgat catagagatt gagatgacaa tgatagagat aatcaaggta acagctgccg
     3061 ccattgagta ttgaggagat gtacctgttg tcaaacggta gatccatgag atcaagatat
     3121 cggttgaacc agctccacct ccgacactac caggtcctcc accattgaag aggtacatga
     3181 tagagaagtt gttaaagttg aaggtgtatt ggctaatcaa agtaggtgcc gcaacagcca
     3241 aaatcattgg gaaagtgatg ttgcggaatt tttgccaagc gttggcaccg tcaatataag
     3301 ctgcttcgta aaggtcgtta ggaatagatt gcaagatacc caaagtcaaa acgtagatgt
     3361 atgggaatcc gagccaacct tgcatcataa tcaaggcaat cttagtccaa gttgggtctg
     3421 ttttccaagg aataagagct ccatcaagga aaggaaggaa tttagccaag attggcaata
     3481 cttgagtgtt gatagcaccg acactatcgt taaacatgtt tgagaatgtc aagatagtga
     3541 tgaaggctgg gacagcccaa ggaagaagga aaataacacc aaagatacgt tttcctttga
     3601 taaatggttg gttggcaatg atagctgtga agataccaat tacgatttgt aaagtagaag
     3661 ctgccaaagc ccaaatgata gtccaagaaa gaacagaacc aaaggcagaa cggaaggtac
     3721 tcaagctcca aatgtttgta aagttggtca aaccaaccca gtccaacaac ttgtttggtg
     3781 gcaagtgttg gaagtcgtag ttggtaaagg cgatcatcaa ggttacgata actgggaaga
     3841 taatcgcgaa ggtcatggca acataagatg gaatgatcaa gaggtaaggg aagccatttt
     3901 cataaatccc tttgatcatg tctttgagtg tgcgtggaac tggaattcca ttgttaatgc
     3961 gttttgcaat cgtatgtgca tctttgatat ttgagaaata aaagagtaca taaacgatta
     4021 caaagattag atggaaggca ccacgaatca gcataaagag ggaattatca cgacctggtt
     4081 tgtcaccaag agtgatgagg ttgctcaatt caggggttgc aagtgctagg aagtaaagga
     4141 caaatacgat ggttacacca aggaagataa aacctttggc tttttgttta ttgtaaatct
     4201 gtcctaaccc aggaatgata gacagcaggg ctgctttact aggttgttgc ttttccatat
     4261 taactccttt cataggatac tactttttta taagaaagct aaattatttg tgtctctatt
     4321 gataaattca aagggggatt tgatttccac cccccttgaa caaatttttt attcaccaaa
     4381 tttttgtttg attgtttctt tgatcaatgt tacagcatcg ttagcagctg ttttagcatc
     4441 tttttgacca cttacagcat caaagagcat atttttcgct ggatcccaaa ctgcagacat
     4501 ttgagagatg tttggcagtg gttgagtgtt cttgaactgt ttgataacag ctgttgtcaa
     4561 ctcatcgttt ttaccttcag cgtatgaacg agcctcagta ttagctggga tttcgttagt
     4621 cttatcatat aatacttttt gttgttcagt tgcaacaagg aagtctacaa atttttgaga
     4681 agcttcaagg ttcttaacgg cttgaggaat gacccaagct ttaccaccac cgaatgcagc
     4741 atattctttt ccatttggaa gagttgggat agttgcaact ccgtagttta ctttagcatc
     4801 tttaaaggct tgagctttcc aaggtccgtc gatgatagca gctgttttac cttcttggaa
     4861 ttgagtttgg attaagtttc cagcaccttc tgtatcttgc atacctttag gccatttttc
     4921 gtaccaagat ttagcgtagt tgatacctgc gatagaaccg tcgtttgcaa gaccgatgtc
     4981 tttagcgtct ttaccgtttt ggccaaagac gtaagcaccg ttaccggcaa gaagtccata
     5041 tgtatagtag aagtttgtcc agtcagctag gaaggcagta gttttaccat cttcaccagc
     5101 gaatgcgtat ttgctatctt tagcaaggtt ttccaagtca gcaaatgttt ttggagcatc
     5161 tttcaccaag tctttgttgt agtacataac aagtgactcg ataacggcag gagcaccgta
     5221 aactttacca ttagcagctg ttacaagaga tttagttgtg tcgtctgttt tagcaccatc
     5281 gctcaatttc acttctgaaa gttgtccgtc agaaccaagg ctacctacac ggtcgtatgg
     5341 agccatcata acatcaggga cattaccaga ttggttgtca agagaaagtt tatcaagacc
     5401 tcctagagca tcaccagttt taagagtgac ttttactcca gcttcttttt cataagcttt
     5461 agcaacctct tcaatatagc tcttatatcc ctcgtctaca tatacagtga gttctttgac
     5521 ttcagatgaa ccagaatcag caggcttatc agcagttttg cttccgcaag ctaccaaaag
     5581 caagctagca agtgtaacag ttccaagcac cgcagtgctc ttcataaatt tagatgacat
     5641 agtgtattcc tcccaaagaa tagcaagttt tattgataag gaaacgcaaa cgttttcctt
     5701 tatgagctta gtatagcaca aatagaaaac ggttgcaagt attttttgta aaaattttaa
     5761 aaaattttta agcttgattt gatagcataa ttttgcattt taatagaaga aaagtatgaa
     5821 atagataaga atacagtatt tttaataaaa atatatggaa tctgtctaaa gagggaaacc
     5881 aggaggggct acctccctgt ttctaatccc tatctatttt ctatagacgt ttgtgctttg
     5941 ttgcatataa attcaagctc cctaaggagg ttttgtactt tgtagactgt ctcgtgagag
     6001 tcaaacttta ggattagata gttttaagtt atctttgcta aaatgagtta gtctccttaa
     6061 aaataatcaa aaaagttttt taaaaacgct tgcaattatg cttgaaaagg agtatactta
     6121 taagtaacgc aaacgtttgc gtctgcaaaa tacgcaacgt tccattattt taacacacga
     6181 ggtgctatta tgaaaaaacg tcaaagtggt gtgttgatgc acatctcttc tcttccagga
     6241 gcttacggaa tcggatcatt tggtcaaagt gcttacgact tcgttgattt cttggtccgt
     6301 acaaaacaac gttactggca aatccttcca ttaggagcaa ctagttacgg ggattctcct
     6361 taccaatctt tctcagcctt cgcaggaaac actcatttta tcgatttaga tatcttggtg
     6421 gagcaaggtt tgttgggagc gagtgacctt gaaggagttg actttggtag cgatgcgtct
     6481 gaagttgact atgctaaaat ctactatgca cgtcgtcctc ttttagaaaa agcggtgaaa
     6541 cgtttctttg aagtcggaga tgttaaagat tttgagaaat ttgctcaaga caaccaatca
     6601 tggcttgagc tctttgctga gtatatggct atcaaagagc attttgacaa tcttgcttgg
     6661 actgaatggc cagatgcaga tgctcgtgct cgtaaagctt cagcacttga aagctatcgt
     6721 gagcaattgg cagacaagtt ggtttaccac cgtgtgactc aatacttctt cttccaacaa
     6781 tggttgaaat tgaaagctta cgctaacgac aaccacattg aaatcgttgg ggacatgcca
     6841 atctacgtag cggaagattc aagtgatatg tgggcaaatc cacatctctt caaaacagat
     6901 gtcaatggta aggctacttg tatcgcagga tgcccaccag atgagttttc tgtaactggt
     6961 cagctttggg gtaacccaat ctatgactgg gaagcaatgg acaaagacgg ctacaaatgg
     7021 tggattgaac gcttgcgtga aagcttcaaa atctacgata tcgttcgtat cgaccacttc
     7081 cgtggcttcg aatcttattg ggaaatccct gctggttccg atacagcagc acctggtgag
     7141 tgggtgaaag gtccaggcta caagcttttt gcagccgtta aggaagaact tggtgagcta
     7201 aacatcatcg cagaagacct tggcttcatg acagatgaag tgatcgaatt gcgtgaacgt
     7261 actggcttcc caggaatgaa gattcttcaa tttgccttca acccagaaga cgaaagcatt
     7321 gatagcccac acttggcacc tgctaactca gttatgtaca caggaacaca cgataacaat
     7381 acggttcttg gttggtaccg taatgagatt gatgatgcga ctcgtgagta catggctcgt
     7441 tacacgaacc gtaaagaata cgaaacagtg gtacacgcta tgcttcgtac agtattttca
     7501 tcagttagct ttatggcaat tgcgactatg caagatttac tagaattgga tgaggcagct
     7561 cgtatgaact tcccatctac ccttggtgga aactggtctt ggcgtatgac tgaagatcaa
     7621 ttgacaccag ctgtcgagga aggtttgctt gacttgacaa caatttatcg ccgaattaat
     7681 gaaaatttgg tagatttaaa gaaataagac aataatcagg agacaactaa acatgttatc
     7741 actacaagaa tttgtacaaa atcgttacaa taaaaccatt gcagaatgta gcaatgaaga
     7801 gctttacctt gctcttctta actacagcaa gcttgcaagc agccaaaaac cagtcaacac
     7861 tggtaagaaa aaagtttact acatctcagc tgagttcttg attggtaaac tcttgtcaaa
     7921 caacttgatt aaccttggtc tttacgacga tgttaaaaaa gaacttgcag ctgcaggtaa
     7981 agacttgatc gaagttgaag aagttgaatt ggaaccatct cttggtaatg gtggtttggg
     8041 acgtttggct gcctgcttta tcgactcaat tgctactctt ggtttgaatg gtgacggtgt
     8101 tggtcttaac taccactttg gtcttttcca acaagttctt aaaaacaacc aacaagaaac
     8161 aattccaaat gcatggttga cagagcaaaa ctggttggtt cgctcaagcc gtagctacca
     8221 agtaccattt gcagacttta ctttgacatc aactctttac gatattgatg ttactggtta
     8281 tgaaacagcg actaaaaacc gcttgcgttt gtttgacttg gattcagttg attcttctat
     8341 tattaaagat ggtatcaact ttgacaagac agatatcgct cgcaacttga ctctcttcct
     8401 ttacccagat gatagtgacc gtcaaggtga attgctccgt atcttccaac aatacttcat
     8461 ggtttcaaac ggtgcgcaat tgatcatcga cgaagcaatc gaaaaaggaa gcaacttgca
     8521 tgaccttgct gactacgcag ttgtccaaat caacgatact cacccatcaa tggtgattcc
     8581 tgaattgatt cgtcttttga ctgcacgtgg tatcgagctt gacgaagcaa tctcaattgt
     8641 tcgtagcatg actgcctaca ctaaccacac aatccttgct gaggcgcttg aaaaatggcc
     8701 tcttgaattc ttgcaagaag tggttcctca cttggtacca atcatcgaag aattggaccg
     8761 tcgtgtgaag gcagagtaca aagatccagc tgttcaaatc atcgatgaga gcggacgtgt
     8821 tcacatggct cacatggata tccactacgg atacagtgtt aacggggttg cagcactcca
     8881 tactgaaatc ttgaaaaatt ctgagttgaa agccttctac gacctttacc cagaaaagtt
     8941 caacaacaaa acaaacggta tcactttccg tcgttggctt atgcatgcta acccaagatt
     9001 gtctcactac ttggatgaga ttcttggaga tggttggcac catgaagcag atgagcttga
     9061 aaaactgttg tcttatgaag acaaagcagc tgtcaaagaa aaattggaaa gcatcaaggc
     9121 tcacaacaaa cgtaaattgg ctcgtcactt gaaagaacac caaggtgtgg aaatcaatcc
     9181 aaattctatc tttgatatcc aaatcaaacg tcttcacgag tacaaacgcc aacaaatgaa
     9241 cgctttgtac gtgatccaca aataccttga catcaaagct ggtaacatcc ctgctcgtcc
     9301 aatcacaatc ttctttggtg gtaaagcagc tccagcctac acaatcgctc aagacattat
     9361 ccatttaatc ctttgcatgt cagaagttat tgctaacgat ccagcagtag ctccacactt
     9421 gcaagtagtt atggttgaaa actacaacgt tactgcagca agtttcctta tcccagcatg
     9481 tgatatttca gaacaaatct cacttgcttc taaagaagct tcaggtactg gtaacatgaa
     9541 attcatgttg aacggagctt tgacacttgg tactatggac ggtgctaacg tggaaatcgc
     9601 tgagttggtt ggagaagaaa acatctacat cttcggtgaa gattcagaaa ctgttatcga
     9661 cctttacgca aaagcagctt acaaatcaag cgaattctac gctcgtgaag ctatcaaacc
     9721 attggttgac ttcatcgtta gtgatgcagt tcttgcagct ggaaacaaag agcgcttgga
     9781 acgtctttac aatgaattga tcaacaaaga ctggttcatg actcttcttg atttggaaga
     9841 ctacatcaaa gtcaaagagc aaatgcttgc tgactacgaa gaccgtgacg catggttgga
     9901 taaagtcatc gttaacattt ctaaagcagg attcttctca tctgaccgta caatcgctca
     9961 gtataatgaa gacatctggc acttgaacta atactcttcg aaaatctctt caaaccacgt
    10021 cagctttatc tgcaacctca aagcggtgct ttgagcaacc tgcggctagc ttcctagttt
    10081 gctctttgat tttcattgag tataagatac aaatttatac taatacatct tgtaaaaaag
    10141 cgagtttcga ttgaaattcg cttttttaaa tgctgtggat ttgggtcaat cttgtctaaa
    10201 aatagggaaa tcctagatac agtgaaggct ttaaatgctg gtttttgctg tcttcagcct
    10261 tatatttttt cgtagttggt tacctcatat ctcttgtatt cgcttacata aagtattata
    10321 atataattgt aggaaagaag gtgtttttat gatatacaca cttaaattgg tgttgtttat
    10381 tacctttctt gtaataagct tgttacctga taagattttt ggaaaaaata aaaaaatttg
    10441 gaaaatagtt tttgcaatat tgacggcagt ggcagcattg tcatttatgt actaagttat
    10501 tttaagaatg tagggaaata aaccctacat tctttttagt tttttctgtt ttctaaattc
    10561 tatttatcca agcgcttcaa catttcttgc ttcttcgctt caagttctgc acgcttttct
    10621 tcgatttcgg catgtttttt ctcgagttca gaacaacttg caccattgct aaattctttt
    10681 cgccatcagg agatagggtg agtcgacatg tctattactc acccaaagca gtcctacaaa
    10741 gcaggaattt tctgttactt ttttggaaat agtaacgttt atacagcttt gacacttcgt
    10801 atcaaagcgc caaacacact ccgagggatt tacagaaagc agaaaaggaa tgatctggta
    10861 taagatcatt ccttttctct ctttttcttt aagtaattat atacaatgta cgacgaagtc
    10921 gtcattgcaa tgctgatcca ccacctaaag ggaactttaa acaacattga taagataaag
    10981 aatataaaca acgaaaatac gttataccca attaatttta ttgtatatct catgattaaa
    11041 agttaatcct tccgttgtta ggaatggcat catttttatc ccataattgt gctaaataag
    11101 tccccggtga taataaattc atagcggatt ctaaagcaac atcatttaca aaccaattac
    11161 ctagatatct agaaattgct gaacgaatag cactttttgc tgcatgtttt ccttttactt
    11221 taattagatt tgcaaggcct gcagtagttc ctcctaatgc taaagctatt gcagtatcta
    11281 atagagcacc catttgatta actgtaatac cttgccaagc tgctctaaat ggagagtatg
    11341 taggtgggat tgtataatcg ccttgtaatt gtcggttaat tacttctttg atccattgtt
    11401 gtgagacgtc tggatgaaaa gattggattt cgtttgcaag tgtattgatt tgttcttctg
    11461 ttagagaagt gacaggttga agttccatat ttgtttcaat ttgtgatact tgttcagaag
    11521 cgtatacagc tgaaacactt ggaatcgctg atacaattaa cacaattgac gtcaaaaaaa
    11581 ccgaaataaa tttcattaat ttgttcatga gcttttctcc tttttatttg tatctgctta
    11641 cattttatta tatactgtta ttatagtcaa gaaaaatatg ctattatgtt aaaaaaatat
    11701 ttttaaaaat ataaatggac ggatttattt tggattttat ttgttatttt gacctgcctc
    11761 tatattggta accatgattt gtttactttc aatcatcaag aattctcttt tcgtggtagc
    11821 gtttggggtc tggtactggc cttatatcac ttattgttca ttgataagtt tgtaatttcg
    11881 aatcgaaaat aaagattaga actatgattg actggctact tttaatattg attttggagg
    11941 aagattttgt ctctattatt tattatttta aatttattta ttttgtataa gatctattct
    12001 ttgaggcggg aaaaatcgaa atacttgatt tatacggcct atatcatatt tggggtaaat
    12061 gtagtatatg gtattcaatg gttattaaga gaactgattt caactatttc cccttaatgt
    12121 gtatggaaac tataacaaaa cgcataagat taaggtttgt gtacctgttc ttatgcgttt
    12181 ttataatatt gaagattttc ggtaagtttt atacctttgg gaagtctagt ttagaatgct
    12241 ttcccaacca aaatctctgc ttcgatggta atctctgtca gttggctcca gtcaatcttg
    12301 ctctggtcaa cgtgaaagag gagaggagtc atactgagta gggcttggag ttgttctgct
    12361 gtgatagtct tagtcagaga ggcagtttga ctagatagga tggtaaagtg ttcctggaaa
    12421 tgctccttga tatcttggtt agaatactcc ttgtttgtca gctggtcctg tactctttga
    12481 cggatttctt tgaggtgatt ttcagttggg ataaccttta tcaagatacc gtctttggat
    12541 aaaacgcgac gaaattctcc atagttggca ggtgagaaga tatcaagcag aatatccatg
    12601 ttagcgtctt ttataggaag tcgtgccaag tcgccaacaa accaattgac tgcccagttg
    12661 ggttcacttt tagcagcgat ttggactgaa tctttggaga tgtcaaaggc atagaaagtt
    12721 ttttcagagt gactttcttg tagtttgcga gaatagaatc cttcaccaca accgatatcc
    12781 aaaattgttg tggtagtttt tgagcttgca agcaagtcag atacagcatc taagatagct
    12841 tggtaaaagc cggcttctag gatttgttga cggttttgaa aattttcctt gttatagtta
    12901 gcagattgct tgatttgagg tgctagattg acatagccaa atttcgccaa gtcaaaagaa
    12961 tgacggttgc agcacttgaa attagtctct aacagagtca gattttcttg acagatagga
    13021 caggcaaagg cagtcgcaga agcaaaacgc tgaagtttgg gcttgagatt tgtattcata
    13081 gtttaagtgt atcaaaagag gcaaatgaaa gcaactttag gaataagtag atgggagatt
    13141 cagtagaaat aaaactaatt ctccaattta tagaatgaaa ttgcttttat atctaaattg
    13201 ctatataata atgataagga ggaaataagt atgttaaaag aagtattaac cgttgcaaaa
    13261 gttgcgaaaa aatcttcatt atttttgggt ggtgtcgcat ttggtaccct tggtttgaaa
    13321 atcttagcaa gtaaggaagc taaaaaaggt tattctaaag ctttggctaa ggcttacaag
    13381 ttgaaagacg agctagatgc atctgtttct gttgtgaagc aacatggaga cgatgtcttg
    13441 caagatgcca aatatttgta cgagcaagag aaaaaagaag agcaattaga tagccttata
    13501 ggtgaataat atgtctttta aagtgctaca tagaggatac caacatatcc gactatcatc
    13561 ttctttttca cttaccttgg atattcaaga ctatcttcgt tccttggcga gagatgaaaa
    13621 ggggattgag tctatccagt tttacatgga tcaacagcac tttactctac gcataaaaga
    13681 aggcttttct gtattagata atgcagaagc ctttttaaaa agaattgata aagggaaagt
    13741 ttctgagttg atgactcttc ccattcgtag agaagagagt gcttattcta ttgtttcagg
    13801 tgcagcggtt aagcgtgtac tttttcgtag ttttgtgccg tatcctattc gctatatatg
    13861 gacttgttat caggctttgg gttatattag agaagcctat caaacactag cgcgtaagga
    13921 actaacgatg gaagtcttgg actgttcggc tattttattg tctttgttta tgaaccaatc
    13981 caagacagct agcaatatca tgtttatgct tgatttgggg aatcatttag atcagtggtc
    14041 cttgaaaaaa actgcaacag atttagaaca gagtcttctt gcaaaagaga gcgatgtatt
    14101 cctagtacag ggcgatacgg ttgttagtat caagagttcc gatgttcaaa taggagatgt
    14161 cttgatctta tctcaaggaa atgaaattct gtttgatgga caagtagttt caggtttagg
    14221 tatggtcaac gaaagttcct tgacaggaga gagttttcca gttgaaaaaa gagagtctga
    14281 tttggtttgt gcaaatacag tattagaaac tggagagtta cgcatttgtg taacagataa
    14341 tcagatgaac agccgtcttt tacagctgat tgagttgatg aagaaatctg aagaaaacaa
    14401 gaaaacgaaa caacgctatt tcatcaagat ggcggataaa gtcgtcaaat ataatttctt
    14461 gggggctggg ctgacttacc tattgacagg ttctttttct aaggctattt ctttcctatt
    14521 ggtcgatttc tcctgcgctt tgaaaatctc tactcctgta gcttatttga cagttatcaa
    14581 ggaagggttg aaccgtgaaa tggtgattaa ggatggagat gttctggaga aatatctgga
    14641 agttgatact ttcttgtttg ataagacagg aacaatcaca actagttatc ctatagttga
    14701 aaaggtgtta ccttttggag actatagtga ggaagatatt ctcagaatca gtgcctgtct
    14761 tgaggaacac atttatcatc ctattgctaa tgccatcgtc aagcaagctg agatagaggg
    14821 aattgaacat gaggaaatgc atgggaaact ccaatatatc gcaagcaagg ggatcaaatc
    14881 tcatatagat ggccaaccag ttcttattgg gaattatgtc ttgatgcagg atgagcagat
    14941 tcatatcagt tcagaacaaa atgctttaat tgaagagtac aagagtcact acaatctctt
    15001 attcttggct tatcaaaatg aattgattgg aatgttctgc attcatactc ctttgagaaa
    15061 agaagcaaaa acagccttgg ataaacttaa ggcacagggg aaaaaattga ttctggcaac
    15121 aggagacacc ttgactagga cagaggaatt agtcaaagat ttgccctttg atcaggtcta
    15181 tacagacttg aaacctgatg ggaaatttga gttagtagag aaactgcaga aagcaggtca
    15241 cactattttg atggttggag atggattgaa tgactcagcg gctttaactc tatcagatat
    15301 cggtgtggtg atgaatgaga gtgcagatat ttctaagcag atgagtgata tcttattgtt
    15361 agataatcgt ttggatttct tccaagagtt ggattcgtta tcatcatctt tgcaaacact
    15421 catcaagaaa aatattcaag ataccgttgt cgtaaatagt agtttgattg gcttcggctt
    15481 atttaattgg ctcagtcctt caaatctctc tatcttacat aatctaacaa ccttgcgcat
    15541 tgtcctgcgt agcctgtcta ttaaaaatag atagatgaga gttattaaca gcaaaaagga
    15601 gttacctttc tcgaggctaa ctcctttgtt tatagataaa tgttgaatag tttagtaagt
    15661 caatacaaag tattttttct tcccacgacg gataacagtc agttcattct ctaacttatc
    15721 agcgtcactc aagacatagt caagctcttg gatgcggtcg ccgtttacgt agatggctcc
    15781 gttttggacg tcttcacggg cttggcgttt tgagttaact ataccagatg agacgagcag
    15841 ttccacgata ttgttgtttt cgtctgcctg tacttggtag tttggcacac cacgaagtcc
    15901 ttgtttgagc tctttgacag aaaggttttt gatgtttcct gcaaagagtt gctcagtgat
    15961 gttaagtgct tctttgtagg cttcttctcc gtgaacaagt gtaacaactt cacgagccaa
    16021 gactttttga gccaagcgtt cgtgtggcgc tgcttcaaat tgtttacgaa tatcttcaat
    16081 ctcatcaagt gacaagaaag taaagatttt caagaagcga acagcgtcag cgtccatcac
    16141 gttcatccag aattggtaca tttcgtatgg agaagtcttt tcgggattga gccagacggc
    16201 atttccttct gatttaccaa atttcttacc agttgcatct gtgattagtg gaacagtgat
    16261 aacgtgacca gtcttgtccg ctttacgacg aagcaattcg gtaccagctg tcatatttcc
    16321 ccactggtca gaaccaccga tttgaagagt gacattatgg tcttggttaa ggacgaagaa
    16381 gtcgtaccct tgcatgattt ggtaagcgaa ctcagtgtaa gaaattcctg tttcgattcg
    16441 tttttttaca gattccttac tcatcatgta gttgaccgtg aagtattttc caatatcacg
    16501 gaggaagtca atgaagctga tgctgccaaa ccagtcgtag ttgttgacca tgacagcctt
    16561 gttttcgcca ttttcaaagt caagaaaacg agaaagttgt ccttggatag acttgaccca
    16621 gccatctact gtgtcttttg tttggagact acgttcagca tctttgaagg acggatctcc
    16681 gatgagacct gtagcaccgc caacgagcgc ataaggtttg tgacctgcta gttgcaagcg
    16741 acgacttgtc aagattgcga caaggtggcc taggtgaagg ctgtcagcag ttggatcgta
    16801 gccagtataa taagaaactt gaccttcttc tagggcttta cgcaaagctt cttcatcagt
    16861 cgtttgaaat atcaaaccac gatcttttag ctcatcaaaa atgtgcatgt gtcttttctc
    16921 ctttttaaaa ttattgtttc tacctattgt atcacaaact tgggcaatag cctagcagaa
    16981 tagggaagaa agtggctatt ttattgaaaa tttccctttt gtggtataat agatagagtg
    17041 aggaaatcca tgcaaaatca attaaatgaa ttaaaacgaa aaatgctgga atttttccag
    17101 caaaaacaaa aaaataaaaa atcagctaga cctggcaaga aaggttcaag taccaaaaaa
    17161 tctaaaacct tagataagtc agccattttc ccagctattt tactgagtat aaaagcctta
    17221 tttaacttac tctttgtact cggttttcta ggaggaatgt tgggagctgg gattgctttg
    17281 gggtacggag tggccttatt tgacaaggtt cgggtgcctc agacagaaga attggtgaat
    17341 caggtcaagg acatctcttc tatttcagag attacctatt cggacgggac ggtgattgct
    17401 tccatagaga gtgatttgtt gcgcacttct atctcatctg agcaaatttc ggaaaatctg
    17461 aagaaggcta tcattgcgac agaagatgaa cactttaaag aacataaggg tgtagtaccc
    17521 aaggcggtga ttcgtgcgac cttggggaaa tttgtaggtt tgggttcctc tagtgggggt
    17581 tcaaccttga cccagcaact aattaaacag caggtggttg gggatgcgcc gaccttggct
    17641 cgtaaggcgg cagagattgt ggatgctctt gccttggaac gcgccatgaa taaagatgag
    17701 attttaacga cctatctcaa tgtggctccc tttggccgaa ataataaggg acagaatatt
    17761 gcaggggctc ggcaagcagc tgagggaatt ttcggtgtag atgccagtca gttgactgtt
    17821 cctcaagcag catttttagc aggacttcca cagagtccca ttacttactc tccttatgaa
    17881 aatactgggg agttgaagag tgatgaagac ctagaaattg gcttaagacg ggctaaggca
    17941 gttctttaca gtatgtatcg tacaggtgca ttaagcaaag acgagtattc tcagtacaag
    18001 gattatgacc ttaaacagga ctttttacca tcgggcacgg ttacaggaat ttcacgagac
    18061 tatttatact ttacaacttt ggcagaagct caagaacgta tgtatgacta tctagctcag
    18121 agagacaatg tctccgctaa ggagttgaaa aatgaggcaa ctcagaagtt ttatcgagat
    18181 ttggcagcca aggaaattga aaatggtggt tataagatta ctactaccat agatcagaaa
    18241 attcattctg ccatgcaaag tgcggttgct gattatggct atcttttaga cgatggaaca
    18301 ggtcgtgtag aagtagggaa tgtcttgatg gacaaccaaa caggtgctat tctaggcttt
    18361 gtaggtggtc gtaattatca agaaaatcaa aataatcatg cctttgatac caaacgttcg
    18421 ccagcttcta ctaccaagcc cttgctggcc tacggtattg ctattgacca gggcttgatg
    18481 ggaagtgaaa cgattctatc taactatcca acaaactttg ctaatggcaa tccgattatg
    18541 tatgctaata gcaagggaac aggaatgatg accttgggag aagctctgaa ctattcatgg
    18601 aatatccctg cttactggac ctatcgtatg ctccgtgaaa agggtgttga tgtcaagggt
    18661 tatatggaaa agatgggtta cgagattcct gagtacggta ttgagagctt gccaatgggt
    18721 ggtggtattg aagtcacagt tgcccagcat accaatggct atcagacctt agctaataat
    18781 ggagtttatc atcagaagca tgtgatttca aagattgaag cagcagatgg tagagtggtg
    18841 tatgagtatc aggataaacc ggttcaagtc tattcaaaag ctactgcgac gattatgcag
    18901 ggattgctac gagaagttct atcctctcgt gtgacaacaa ccttcaagtc taacctgact
    18961 tctttaaatc ctactctggc taatgcagat tggattggga agactggtac aaccaaccaa
    19021 gacgaaaata tgtggctcat gctttcgaca cctagattaa ccctaggtgg ctggattggg
    19081 catgatgata atcattcatt gtcacgtaga gcaggttatt ctaataactc taattacatg
    19141 gctcatctag taaatgcgat tcagcaagct tccccaagca tttgggggaa cgagcgcttt
    19201 gctttagatc ctagtgtagt gaaatcggaa gtcttgaaat caacaggtca aaaaccaggg
    19261 aaggtttctg ttgaaggaaa agaagtagag gtcacaggtt cgactgttac cagctattgg
    19321 gctaataagt caggagcgcc agcgacaagt tatcgctttg ctattggcgg aagtgatgcg
    19381 gattatcaga atgcttggtc tagtattgtg gggagtctac caactccatc cagctccagc
    19441 agttcaagta gtagttctag cgatagcagt aactcaagta ctacacgacc ttcttcttca
    19501 agggcgagac gataattctc taaatgaagt ggccaatcaa tggattgcca cttttttctt
    19561 ttcgtgttac aataagggta tgaatcagta tcagaaaaag attgttaacg gaaaaattta
    19621 ttcgctccta tccggcttaa tatggggaat ctgtggaatt ttaggagagt acttctttac
    19681 tcattatcag gtgtcttcgg gctggattac ctctatgcgt ttgacactgg cagggagtct
    19741 tgtactcatt tggtctgcaa tacaattaaa atcgcaagtg ctagatattt ggcgagacaa
    19801 gaaaaattac ctgccctttt tagcctatgc tattttgggg attttttcag ttcagtattt
    19861 tttctatctc tgtgtagaat actcaaatgc tacgacagca actattttac agtttattag
    19921 ccctgtcttt atcctctttt acaatcgctt ggtttatcaa aaacgagcgt caaaaagcgc
    19981 tgttttctat gttttggttg ccatgctggg tgtttgcttg atggcgacaa agggagatct
    20041 ctctcagtta tccatgacgc cgctagctct tataacaggt ttgctgagtg ccatgggtgt
    20101 tatgtttaat gttatcttgc cccaaccttt tgctaagcgt tatggttttg ttcctacggt
    20161 tgggtggggg atgattttgg caggtttgtt tagcaatgtc ctctcgccgg tttatcagct
    20221 ttcctttact cttgatattt ggagtatctt gatttgcctc attatcgctt tctttggaac
    20281 ggcttttgct tttttcattt ccatgaaggc tgtgtccttg gtttctcctt tggtggttgc
    20341 cgttatcagt gccagtgaac ctctctcttc tgctctcttg agtgttttgt tcttaggatt
    20401 agtagtggat tggtccctcc ttctagctat agccttgatt attttaccca tgatattttt
    20461 gtctatagaa gaagcgaaag aaagtagata aaaagtcttt tcttaattct aaaagtgtgc
    20521 tatactagaa tagtcaataa aacacgggga gatagttgtg aagaatgttt ttaggttgta
    20581 tcggtagggg cttgctttta ccgacagcac gttttatctc acaaccctaa aaaatcatac
    20641 ctaaaaacag acaaaaaggt ttcaaatttg aggccttttt tggtagaata ggtatcatta
    20701 taacgaacca ggaggcacct atgactgcta caaaaatgaa cgctcaagaa attatccaat
    20761 ttatcgccaa tgctgaaaag aaaaccagtg tcaaagtaac ctttgagggg caactcgcaa
    20821 ctgctgtgcc tagctctgtt gtcaaactag gaaatgtcct attcggagac tggaaggacg
    20881 tggctccgct tcttgaaggt ttggtagaaa atcaagatta tgttgtcgag caagatgctc
    20941 gtaattctgc agttcctttg ctagataagc gtgctatcaa cgctcgtatc gagccaggtg
    21001 ctattatccg tgaccaggtg gaaattggtg acaatgctgt tatcatgatg ggagctgtta
    21061 tcaatatcgg tgctgaaatc ggtgctggaa ccatgattga catgggtgcc atccttggtg
    21121 gccgtgccat cgttggaaaa aatagccacg ttggtgcagg tgcagttttg gcaggtgtga
    21181 ttgagccagc tagtgctgaa ccagtccgtg tcggagacaa tgttcttatc ggtgctaatg
    21241 cagtggttat cgaaggagtc caaatcggta gtggttcagt tgtcgcagca ggagctattg
    21301 ttacccaaga tgtcccagaa aacgtggtag tagcaggtgt tccagctcgt attatcaaag
    21361 aaattgatgc ccaaactcaa caaaaaacag cgctagagga tgcgcttcgt accttgtaat
    21421 tgtaaaagta aaaaagaggc ggaacccttt ttccagcctc tttctgctat atcggaggac
    21481 agatagatgt tagatttgat tcagactaga cgagatttac accagatacc agagattggc
    21541 ttggaggagt tcaagactca ggcttatttg ctggatgtga ttgagaaatt gactacgggc
    21601 aaggattttg ttcaaattcg tacttggcgg acagggattt tggtctacct gcagggaagt
    21661 cagccggagc gaaccattgg ttggcggaca gatattgatg gcctgcctat cgtcgaacaa
    21721 acaggtctac cttttgcttc tcaacaccaa ggtcgcatgc atgcctgtgg ccatgatttt
    21781 catatgacca ttgccttagg ctgtcttgag cgcgcccttg aggagcaacc taaaaataat
    21841 ctgctctttc tatttcagcc tgctgaagaa aatgaagctg gtggtatgct catgtatgag
    21901 gctggtgctt ttggagattg gttgccaaac caattctatg gtctccatgt tcgtccagat
    21961 ttgaaagtcg gccagattgc gactaatact catacactct ttgcaggaac ttgcgaggtg
    22021 aagatccgtt tcaaaggcaa aggtggccac gcagcttttc ctcatgaagc caatgacgcc
    22081 ttggtggcgg ctagttactt tgtgacccaa gtgcagtcag ttgttagccg caatgtcaac
    22141 ccaatcgagg gagcagtggt gaccttcggc gtttttcaag ctggaaccac caacaatgtc
    22201 atcacagaca cagctttttt acatggaact attcgtgcct tgacacagga catgagtctc
    22261 ttggtgcaaa agagggtaaa aacagttgca gaaggggttg cagctgcctt tgatatggaa
    22321 gtcgaagtgg aactcaaaca aggtggatac ctacctgttg agaacaatcc agccttggcg
    22381 tgtgaactga tggacttctt tgacgaaaaa gacggaatcg agttgattga tatcgaacct
    22441 gctatgacag gcgaggactt tggttatctc ctttcaaagg ttgatggcgt gatgttttgg
    22501 ctaggtatcg atagtcccta cgctcttcat caccctcaaa tgagtcctaa ggaagaagtc
    22561 ttagctattg gggtggctgc ggtgtctagt ttcctgaaaa agaaggcagc agaatagagg
    22621 gcttgtctat gaaatcggaa ttacgcaagc aagtcttgca tgaaatgaag gctatatctc
    22681 aagagcaaaa acaggctata gaccaagctt taaccgagcg gcttttacac catcccttct
    22741 accaagaagc caaggtcatc gcaacctacc tctctttttc tcatgagttt caaacgcggg
    22801 aactgattga gcaggcgctg aaggacggca agaaggtttt aatgcccaaa acttatccca
    22861 aggggcgcat ggactttgtg gtctatgatc cgcaacagtt ggtaaaaact tcctttgcct
    22921 tactggagcc acagggagat gtggaagtgg tggatgcatc tcagattgat ttgattcatg
    22981 ttcctggtct ggcttttacg acgaaaggat atcggatagg atacggtgga ggctattatg
    23041 accgctatct ggaacatttt tctggtcata ctttgagtac ggttcatcct tgtcaaattc
    23101 aggactttat acctgaaaac catgatattc ctgttcagga ggtattaatt gatgaaggaa
    23161 atctttgata gacgttaccc tgtgacgagt ttcttcctct tagtgacggc cttggtattt
    23221 ttactaatgt tggtcactgc aggcggaaac tttgacaggg cagatacatt atttcgattt
    23281 ggagccatgt atgggccagc tattcgcctc tttcccgagc aggtttggcg tctcttgtct
    23341 gccatttttg ttcatattgg gtgggaacat ttcattgtta atatgctttc actttattat
    23401 cttggaaggc aggtagagga gattttcggc tctaagcagt tcttttttct ctatctctta
    23461 tcaggaatga tgggcaatct ctttgttttt gtatttagtc ctaaatcctt agcagcaggc
    23521 gcctctacct ctctttatgg gctatttgcc gcgattattg ttcttcgcta tgcaactcgc
    23581 aatccttata tccaacagct agggcaatcc tatctgacac tttttgtggt taacattatt
    23641 ggaagtgttc tgattccagg aatcagccta gcaggccata tcggtggtgc agttggtggc
    23701 gcatttctag cagttatctt tccagttaga ggagaaaaac ggatgtataa caccagccag
    23761 agattaggag cggtagtctt gttcgtagga ctcgccattt tgcttttcta caagggaatg
    23821 ggattgtgac gactaatact caatgaaaat caaagagcaa actaggaagc tagccgcagg
    23881 ttgctcaaag cactgctttg aggttgtaga tagaactgac gaagtcagct caaaacatgg
    23941 ttttgaggtt gtagatgaaa ctgacgaagt cagtaaccat acctacggta aggcgatgct
    24001 gacgtggttt gaatttgatt ttcattgagt ataatatgta aacttgaata agaaaaaagc
    24061 tacttaaaaa atgagtagct tttaatgtgt tttggaacca ttcgaaacaa cacagctcta
    24121 aaatagttat tagcgttgtg gaggttctgc aatgccgccc cccaagtttc caaggtaatt
    24181 cccattttcg tcaaacacat ctccattact ccgattatga taagtatctc ttacatgtcc
    24241 actagcatca atgcttctat attctgtata gccattattt gctccaatgt ttgaatttct
    24301 agatgaatat tgttctgatg atccaccatt tgttgaacta tttccagaac cagaataatc
    24361 ttgaccgcca taattatttg aatatccatt agtttgttca gatgaactat aatctacatt
    24421 tgaattggaa gatccattat aagactcatt agaagcacta ccagaagaat tactttgact
    24481 agaagattcc tttgcttttt cagcagcttt ttcttcttct ttcttcaaaa gttcgatttg
    24541 tttttgatat ttatcaacaa gatcatttag agatttaaga gtttcttcca aattctcttg
    24601 tttaaaagtt tcatcaactt ttttgataaa atcaatttgt tttgtaattt ctttcacatt
    24661 tgtttcaaga gttgctttat ctttaaactt ctcatcagaa tctttaacat cataagccaa
    24721 acctttttgc aaatgaaaat ctacaattgc tttagatgat tctttgactt tgttattaag
    24781 atagtcttta cctgcatcgg ttttaagtga agtttcagat agttctttta atttagaaat
    24841 agattcatct gaagatgcat caaatgattc aaatagttta tcaacttgtt ctttgtctga
    24901 tttgctatct ttttctaatt gtacaatttt tgcttgttgt tctgcttgtt gttggttata
    24961 tgaataccat ccaaacccta atatcaatac aacaccaact gcaactgatg aaatgattac
    25021 ttttttattg ttttcgataa attctacaaa tttgttctcc ataatctttt actttgatag
    25081 tgtccgcaat tactttaaaa ttaaaattac acctatcttt ctcccttatt atatttttat
    25141 taatagcttc cacaaaatta aacaacttat gttcggctcg gttgatttaa tttaaccatg
    25201 actactccaa cagttaagtc aaatttaaaa ttatttatta ttttggaacc attagaaaca
    25261 acatagcaaa gttaaaataa ggctttgtcc gtacacaact tgaaaaagtg cgcaccgatt
    25321 cggtgctttt ttgttttact atactaattt attctactct tattttaatg aaaaaacaaa
    25381 tacaagtata gcgttttcat tagtttttat taaataggta gattcaagta gagaatttac
    25441 ttaatttatg tgtggctaat ctttatataa atgattttgt tattccttct cagtcaattc
    25501 ttttccaagt tggatgaggt aattcttcaa atcatctttg acttgtggat gtttgagggc
    25561 gtagtcgatg gatgttttca tgaagccaaa cttgtctccg acatcgtaac gagccccttt
    25621 gaactcacga gcaaatacac gttgtgtttt attgagggtg tcgattgcat ctgtcagctg
    25681 aatttcattt cctgcacctg gagcttgctt ttcgagaatc tcaaaaattt caggcgtgag
    25741 gaggtagcgt ccgataatag caaggtcgct aggagcgtcc tctggagctg gtttttcaac
    25801 aaaggtttca acactgtaaa gaccatcttt tccttcgcct tgcggagcaa taaccccgta
    25861 agcagatact tcgtcatgag ggactggcat gacagcgata gtagacgcgt gggtacgctc
    25921 gtagtcatcc atgagttgtt tggtaagtgg aacagccttt tcgtctgtga tatccatcaa
    25981 gtcatcacca agcataacga caaaaggttc atttccgacg aaagccttgg cttgcaaaac
    26041 agcatctccg agaccgcgtg gatgagtttg gcggataaaa tgcagacgca tgccagttgt
    26101 ttcatcaact agcttcaaaa gatctgtttt ccctttttct ttgaggttat attccaattc
    26161 gaagtttgaa tcaaagtggt cctcaataga acgttttgat ttaccagtga caactagaat
    26221 atcttcaata cctgatttga gagcttcttc cacgataaac tggatagttg gtttgtctac
    26281 gattggcaac atttctttgg caagggcctt ggttgctggt aaaaatcgag ttcctagtcc
    26341 agcagcaggg atgactgcct ttctaacttt tgatgtcata aaaatccttt ctatagaggg
    26401 ttaagaccac tcattttctg ctttaaattc attgttcatg atgtcataaa tggcatcttt
    26461 gatattggtt ccgtgataaa taacttggta aatagcctgt gtaatgggca tatagactcc
    26521 aagttcttgg gctagttcat aggctgctcg agtcgttgaa attccttcga ttaccatgcc
    26581 catattagct tctatatcag ctagggattc tcctcgtccg agagcatctc cagctctcca
    26641 gttacgagag tggatggaag ttcccgttac gatcaaatct cccacaccag ataagccgct
    26701 ataggtcaat ggactggccc cgagtgctac ccctaggcgg gtgatttctg ctaaacctcg
    26761 agcgatgatg gctgccttag cattatcacc aaatccaaga ccatgtaaag ctccagcacc
    26821 gacagcaata atatttttaa gagcaccagc agtttcaacc ccgataacat ccgtattggt
    26881 ataaagtcgg aagtagtgat tactaaatag ctcctgaacg tattgagctg tttgtaaatc
    26941 tttagaagca gcagttatta aagttaggtc acgcacaatg gtctcttctg catgactagg
    27001 ccctgaaaca acgacgatat cactacggag atgttcagga atttcttctt caagaatggt
    27061 tgataatcgt ttatggctat caggttctaa tccctttgat gcgtgcatga tgataacctt
    27121 atggtccaag gtttgtgcaa cttgctgggc aacaagtcgt gtcacttttg ttgggacaac
    27181 aaacaaaatc gcatccacat ctttcaatgt ttctgctaag tcggtgtagg caatgatatt
    27241 ttcgtctaga acgacatctt taaagtagtg cttattagta tggtgtgtat taatttcatt
    27301 gatttgctcg ggaagatttc cccaaatacg tacctcgtgt ccattgtcat ttaagacttg
    27361 tgaaagggcg gttccccaag aaccaggccc caagacggcg acggtttgtt tttccatgat
    27421 ttcctccttg atagagttct ttctcttatt ctatcatatt ctaggggatt ttgcacttgc
    27481 attactgggg tatggtggaa aaatacacaa aaaccgagac gaagggataa actcttagtc
    27541 tttagttaaa gtgagaagaa atctgataga tagtttgatt cagtatatgc tataaataga
    27601 ggtaaacaac tcaggaggtt cttgtaggtt gcgagagttt ggcgaaaaaa ttaaaagatt
    27661 acgtttggct aaaaaaatca gtcgttcaga attttgtggt gatgagtctg aattaagtat
    27721 ccgtcaatta attagaattg aaaatggaga atccagacca acactaacaa agttaaaata
    27781 tatcgctgaa cgtttggggg gtgaagatta caagttgatg ccaagttata tagagttgga
    27841 taaggaatgc ctagaattga agtatttctt gatgaggact cctacatacg aagatgaaac
    27901 tatcgcccaa aagaaagaga gtgttttgat aagatttttg aagagtatta tgataggcta
    27961 cctgaggaag aaagatttat catcccaaat tattcatatc tggcactagc gaactacaca
    28021 gttcaaaaat taccagaaaa gctagttgaa atactgtcct tctggtgatt ttgcttaatt
    28081 tctgatgttc gagacataat tgaggagatt tgtcttgatt ttttcctaaa aaagggcaga
    28141 ctcctctctt ggtttgatgc tcgattttta aaataaggtg acttattctg ttaggcatgt
    28201 tttcccctgc cctctagctt tatatggaac agggagtatc agatttatct ggcagattgt
    28261 atcaaataag gcttgaaact gttttgaata ggcgtataga cttgagaatt tgagaatatg
    28321 tcgtctagca gtagagacat attttccggc aatatgaagg aagagacgtc gaaaacgctt
    28381 gatagtcaag acctttactt catcaccagc tagctgcttt aaaaagaggt agagattata
    28441 agccagacag cccatcatca tacgtacttc attcttaatc atggtcgaac tatctgtctt
    28501 atccccaaag aatcctgctt tcctttcttt gataaagttt tcagcattcc tttgttcacg
    28561 atagaattga aacagctcct cactatctac actgcttaaa ttggtcaaat gaactttttt
    28621 acaaccataa aaaagagcac ttaatcgtac tcttctcttt taaggattaa aggattagtt
    28681 gaattaattc aaatccacta gttctcctgt ttctaggtag actaggcgtt cacaaatgtt
    28741 agcaatgtaa tcagcgaagc gttccagatg ccctatgata taaaggtatt gtgttccatt
    28801 gggaattgag gtttcttggt ctttcataag tccaatgatt tcctttgata aggcataata
    28861 atattggtca atctgttcat ctttttgagc aatactaata gcttttgagg cttggtgcaa
    28921 aggaaaggca accaataaat cggctagcat gctgagggat aatttaccca tttggtgtaa
    28981 ctgttcttcg tcaggggcta gttgattttc ttttagttgc aaaacagctt tggcaatgcc
    29041 tgccatatgg tctcccatac gttcaaggtc tgaacaagaa gacatgatgc taatcacaaa
    29101 tcgaaggtca gacacttgtg gctgctgcaa ggccaacaaa cgggcacagg tcaattcgat
    29161 agcgctttga ccttggttga tagcatgatc cttattgata attagctctg ccatctcctt
    29221 gtctttggag gctaaggcca gtaaggcttt tgaagctgtt tcaaggacaa gttgccctag
    29281 tcctaaaaag gattgttcta attcatgcaa ttctaagtca aattgatttc tcataggttt
    29341 ttccttcctt atccgaaccg tcctgaaata tagtcttctg tgcgctgatc ttttggattg
    29401 gtaaacacgt caacggtatc tccaaattcg caaatttctc ctgttaagaa aaaagcagtt
    29461 ttatctgaaa tacgtgaagc ttgttgcatg ttatgggtaa caatgataat cgtataatct
    29521 ttttttagtt gctgaatgag gtcttcaatt tttaaagtgg agatagggtc taaggctgaa
    29581 gtcggctcat ccattaacag aatatcaggt tctactgcta aagctcgcgc aatgcaaagg
    29641 cgttgctgct gaccgccaga taaggacatg gcactctttt taagatcatc tttgacttct
    29701 tcccaaatgg ctgccccttt taaagatttc tccactaagg catctaattg ttttttgtct
    29761 cgaataccat gtgtccttgg gccataagcc acgttatcat agatagacat ggcaaaggga
    29821 ttaggctgtt gaaaaaccat ccctacacgc ttacgtagct gattaaggtt gaatttgcta
    29881 ctataaatat cttgctcatc taagaggact tggccttcaa tatggcaaga aggaaccaaa
    29941 tcgttcatcc ggttaagggt ttttagaaaa gttgatttgc cacaaccaga tgggcctatc
    30001 aaggcagtaa tctgtctttc tggtaattga atcgaaatat tttttaaggc ttgaaaatcc
    30061 ccgtaaaata agtctaggtg tctgactgaa aatgttccca tactaggaag ctcctttcac
    30121 aagtttccga gataataagc ttgatagagt atttatcatt aaaacagtaa taattaaaat
    30181 cacgccggta gcataggctt cattgacatg tagcccctca cttgacagca tatacatatg
    30241 tagggctaga gaacggcctg aagacatgag actacttggc gtattggtag aggtacctaa
    30301 tgtatacatg agggcagctg tttcaccaac gatacggcca atagctagta tcactccagc
    30361 taaaatacct ggcatggcaa ctggtagaac aattctaaaa acagtccgta acttacctgc
    30421 cccaagtcca taacttgctt gacgcatgct atcactaaca gataaaaggg cttcttctgt
    30481 tgagcgaata atgactggca acaccatgat aactgaggtt aagattcctg ataacagaga
    30541 gtattgaaaa cctaagaaga ctacaaagaa gagcatgcca aacagaccaa aaacaatgga
    30601 aggaatccca gacaaggtat ctgaggccaa tcgcatgatt ttaacacaaa gggaatcttt
    30661 ttttgtatat tccacaagat aaaaaccagc aaaaatccct atgggcaaag ctaaaagaag
    30721 agcaccaaaa accagaataa cggtggaaat aatcgctggc ataagggaaa tgttctcaga
    30781 agtataagtc caagaaaaga gggatagact tagatgaggt aagcctttga tgaggataaa
    30841 accaatgatt aaaaagagag agccaaaggt taaagctgaa aaacaataaa cgagaagttt
    30901 tagcaggtat ttactcataa gatgattttc ctttcaagta ggcaaagtag gcattaatca
    30961 agagaataag gaaaaagaga actgctgagg ttgcaataag ggcttcccta tgctgacctg
    31021 atgcgtaagc catttccaga acaatattgg ttgttaaggt tctggttcct gaaaagagtc
    31081 cacttggaat aattggctgg ttgcctgcca ccaaaataac tgccatggtt tcacctactg
    31141 cgcgaccgat tcctaaaata actgctgata aaataccaga tctcgcagct ggcaagatga
    31201 cactaaaaat actccgttca tgactagctc ctagagccaa gctaccagaa taatacgttt
    31261 tgggaactgt tcggatagca gattctgaca aactgataat ggttggcaaa atcattattc
    31321 ctaatagtaa cgaagcggtt aggacactca tgccatttcc taaaaaggtt ctaatccaag
    31381 gcaccaataa ttgtaggccg aaaaaaccat aaacaataga tggaatggct gccatcaagt
    31441 tgatagctga ttttaagaag ccatagacgg gctttggaca ataataaacc ataaacaccg
    31501 atgtcaagat gcctgttggc accccaatca caatcgctcc taaggtaatt aataaggaac
    31561 caacgatcat tggtaaaata ccatagcttg ccggaatgtt cgttggcgac caatcactgc
    31621 ctaataaaaa acgggcaaag ccgtagttag ccatgaaagg taagccatta ctaaaaataa
    31681 agaaacagat tagcaaaata gctacaacag ctactgttgc actcatgaaa aaaattgccc
    31741 taaaaactgc ttctttgaag gcttgttttg tcacatcttg tcctttctag tgaagaaagt
    31801 aagggagata cgacacctcc ctacttgcct tctttatctt attgtacgat gaaacgtctg
    31861 catctcttta gagatttatg gagcaaacat tttatttaat cttgtcccag gtggttaatt
    31921 tgccactaaa aacgtctgca agttcagcca tactgacttg gcttgcctta ttgtcattat
    31981 tgaccacaac agcaataccg tctaaagcaa tagcatcatg ggtgagactc ttaccttctt
    32041 caggagttaa ttccctagaa accataccaa tatcagcggt tttctcctta acagcggtaa
    32101 tacctgctga agacccatta gaggtaatat caatcgtaac ttctggattt tcttttttat
    32161 aagcttctgc taatttttcc attaaagaag atactgaagt ggaacctaca acagacaact
    32221 tgcctgataa gtgttggctt gtatattctg tggtttcagt tttagcttca ataaatttat
    32281 tatctgtgac cacttgttga ccttgtttgg agtggataaa gctgataaaa tcttgaccta
    32341 gcttggaaag attagaagac caaacaatgt tgaagggacg ttgaagaggg tattcaccat
    32401 ctaaaactgt gtctcgacta gccttgacac catcaatctc taaagccttg acagatttcg
    32461 ttaaagatcc caaggagatg tagccgatag cattagcatt cccttgaact gctgagagaa
    32521 caccttctgt actattttga atcacagctg ttttggcagt gttgtcaatt tttttatcac
    32581 cgtctttttt gagaatccct gtgatttctg tgaaggcacc ccgtgtccca gagccatttt
    32641 ctcgtgaaat cacctcaatc gttcctgaag ctgactgttt ggaagtagat gactgattgc
    32701 cacaggcaac aagcccaaat cctgataagc caatggctgc aagagtaagt atttttttga
    32761 atttcataat aatcaccttt atctctatgt atttttcttg tgtaggctta ctacatttat
    32821 agtctaacaa gtctttgtaa aggtttatcc ctgattcatg taaagattgt gtaaagaatc
    32881 aaaaaaagcc acttttgaaa aatggctgct cctaaaaata gctttaaaaa ttattagtcc
    32941 tgtgcgaaag attggttagg aagaaaaatc gtgaagcaac tgcctctgcc aagctgactc
    33001 gtcaccgtga cttggccacc taataattga ctgagttctt tgacaatggc aaggccaaga
    33061 ccagtgccac cagtttgtct gcttcgacct ttattaactc ggtaaaaacg ttcaaaaata
    33121 cgatcctgct ctaattgact aataccaatc cctgtatctg atacagaaat cttaatgcct
    33181 tcgttcacct tttgggtctt gacctcaatt tttccccctt gttcagtgta acggatggca
    33241 ttggataaaa gattgagtaa gatttgggaa agtaattgac tatctgatac gagggtgaca
    33301 tcatctggca cctgcacctt tagctgtaaa tccttcttct tgagctgagg ttgcaagctt
    33361 tgagtcaaat cctgtacaaa ttctgccaaa gaaagggtcg tccattgtat aggcatttgt
    33421 tgagccttag ataaggtaag aagatgctca acaatatgct caagacgcaa gctttctttg
    33481 taaataatgt ctagaaagtc atccttgagc gcttcttctt cagctgacat ccccttaatg
    33541 gtttcagcaa agcccttaat cgaagtaact ggtgtcctca attcatggga ggcatttgag
    33601 acaaaggcta aatttaactt ttcataagtt ctaatcgttg ttaaatcata tagcaagacg
    33661 agcacagctt ccacagattg ggtggggcta aaaacgggaa ctgctgtcac ttctaaaatc
    33721 aagtcaccct catgaaaccc acttacttct tgttttaacc ttgttttttg atcaaaggct
    33781 tggtgaacta aattccgaat atccatccgt ttgaggtcat caagtgaact tatgtcgccg
    33841 tccacatcgg gaaaataatg aggcagagag cgactggata ataacatctg accttgagcg
    33901 gaaactaaaa acgtccccat ggttaggtgc gacagaagaa cctccattgt ttcggctagg
    33961 tccttgtatt gctgatcctg ttgggagact ttggttttta ggccagacac atactgagcc
    34021 aaagacttta agtcttcttg ccctttttct aaaaagtatt cactactggt caagagaggt
    34081 tggtgcaagg tctcaaaagc aacttcccat ttccaaaggc aaaagagcca gtagccacct
    34141 agtcccaaag aaagggctag aagaaagaga ccgatgcctt tactgatcca agttaatgcc
    34201 atccctgcaa tcagaatgag gctaacactt agattgacta gccaaaattg aaggtagcgt
    34261 ttcatctata actccttgaa cttataacca taaccccgaa tggttcgaat aaattgaggg
    34321 gctttaggat tgtcttcaat tttttccctc aacttaccaa tatgaacgtc caccaaacgt
    34381 gtttcctgcc caaagtcata cccccagata cgttccaaaa gacgctctct agtcagtgtc
    34441 atgttgggat gtttcataag atagagcaag agttcaaatt cttttggggt caaactcagt
    34501 aacttattcg ccttgtagac ttcatgacgc tcagggtata ctttcaaggt cccaaatagc
    34561 caagaattgt cagcgatatt atctgaatca tctccttctt gttttccttt agttcgcctg
    34621 aggacagcct tgacacgcgc cagtaattct ctagggctaa aaggcttggt caggtagtca
    34681 tcagccccta attccaaggc caaaacctta tcaaattcat cacttttcgc agaaaccatc
    34741 ataattggag ttttgacgcc tttggctctc agccgcttac aaacttctat gccatctaat
    34801 tgtggtaaca tgatatcaag caagataaaa tcaaagggtt ctgtttctgc caaagctaag
    34861 gccttccgtc catttgttac caattgagta gaaaagcctt ccttacttaa atggtagtca
    34921 agcaatttca gaatgtgttc ttcatcatcc actaataaga cttgttttgt catctattat
    34981 ctcctattgg taacattata acacaattat cagaaatcct aacattgcta aatcagatta
    35041 aatttgctta tcaagactag tatctggtca aacgctcaat catctccttg tgctctggat
    35101 aggtcgccag tagatctacc ctttcaaata attcaaaatc ctcaaattca aaaccaggag
    35161 caacaagaca agaaaccaga gcatcatcct tatcaactgt tgatccccaa atagtgccct
    35221 taggaacaca gtagtgaagt tgttgccctt tggatatgtc caggcctaaa gtgactgctt
    35281 cgtagtgacc atctgctgta atcatgtgaa cagtaagtgg ggattctgca tgaaaatacc
    35341 agatttcatc tgctgtcaat cggtgaaaat gtgaaggatt cgtttcttct aataagaaat
    35401 aaatactggt ataaagcgcc cttcccttac cagcaaggtt tatagtgtct gaagcttttt
    35461 ttgtttgtct aaaatagcca ccttcaacat ggggagctaa ctctagagtt cttatcaagt
    35521 cttctttatc cgtcggagcc aatgggttga agtaactctt gttcaaagtg gttttacgat
    35581 ttcaagaact cctctcagtt ctgaggacac ggtaatgatt gatgcgacgg aagtacaaat
    35641 caatcgccct aaaagaagaa ttagcgaatg attctggtaa aaagaaatg
//
LOCUS       DAVBDG010000017        35301 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBDG010000017 DAVBDG010000000
VERSION     DAVBDG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.017"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            207..1649
                     /gene="gtfA"
                     /locus_tag="VQ526_000778"
     CDS             207..1649
                     /gene="gtfA"
                     /locus_tag="VQ526_000778"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2554523.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKHLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            1787..2980
                     /locus_tag="VQ526_000779"
     CDS             1787..2980
                     /locus_tag="VQ526_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2554524.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            3667..5646
                     /locus_tag="VQ526_000780"
     CDS             3667..5646
                     /locus_tag="VQ526_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2554525.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            5713..7209
                     /locus_tag="VQ526_000781"
     CDS             5713..7209
                     /locus_tag="VQ526_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554526.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            7209..8135
                     /locus_tag="VQ526_000782"
     CDS             7209..8135
                     /locus_tag="VQ526_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554527.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            8144..9211
                     /locus_tag="VQ526_000783"
     CDS             8144..9211
                     /locus_tag="VQ526_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554528.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            9222..10148
                     /locus_tag="VQ526_000784"
     CDS             9222..10148
                     /locus_tag="VQ526_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554529.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQSIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            10639..11154
                     /locus_tag="VQ526_000785"
     CDS             10639..11154
                     /locus_tag="VQ526_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2554530.1"
                     /translation="MGILLTSLISLHQLKADKKRDVFRIGISQFITHQSLDATREGFV
                     DELAKQGYVEGENIEIDLQNAQGEQRNLKTISQQLAESSDVVLAIARPSAQSLANTTQ
                     TTPVIFSAVTDPVSAKLVESREHPGGNVTGTSDQSSDAISTQINLIKKVLPKAKTIGI
                     LYTQSEPNSVV"
     gene            11176..11577
                     /locus_tag="VQ526_000786"
     CDS             11176..11577
                     /locus_tag="VQ526_000786"
                     /inference="COORDINATES: protein motif:HMM:NF016292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554531.1"
                     /translation="MKEKGFTVVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISST
                     METVKQVSIKHKVPVFGGSTEMIAVGDLYNYGTNYEELGRQTARMLIRVLKGEKPENI
                     AVELPEKLELHTNQEMADALGIDISKLEGKE"
     gene            11589..12466
                     /locus_tag="VQ526_000787"
                     /pseudo
     CDS             11589..12466
                     /locus_tag="VQ526_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            12479..13282
                     /locus_tag="VQ526_000788"
     CDS             12479..13282
                     /locus_tag="VQ526_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2554532.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFIYVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQYPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(13315..14025)
                     /gene="treR"
                     /locus_tag="VQ526_000789"
     CDS             complement(13315..14025)
                     /gene="treR"
                     /locus_tag="VQ526_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2554533.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            14210..16177
                     /gene="treP"
                     /locus_tag="VQ526_000790"
     CDS             14210..16177
                     /gene="treP"
                     /locus_tag="VQ526_000790"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2554534.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            16355..17980
                     /gene="treC"
                     /locus_tag="VQ526_000791"
     CDS             16355..17980
                     /gene="treC"
                     /locus_tag="VQ526_000791"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2554535.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            18122..18370
                     /locus_tag="VQ526_000792"
     CDS             18122..18370
                     /locus_tag="VQ526_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2554536.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            18577..19371
                     /gene="racE"
                     /locus_tag="VQ526_000793"
     CDS             18577..19371
                     /gene="racE"
                     /locus_tag="VQ526_000793"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2554537.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            19368..20339
                     /locus_tag="VQ526_000794"
     CDS             19368..20339
                     /locus_tag="VQ526_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2554538.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            20315..20836
                     /locus_tag="VQ526_000795"
     CDS             20315..20836
                     /locus_tag="VQ526_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2554539.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            20833..21294
                     /locus_tag="VQ526_000796"
     CDS             20833..21294
                     /locus_tag="VQ526_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2554540.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDTEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            21285..22025
                     /gene="xerD"
                     /locus_tag="VQ526_000797"
     CDS             21285..22025
                     /gene="xerD"
                     /locus_tag="VQ526_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2554541.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            22025..22753
                     /locus_tag="VQ526_000798"
     CDS             22025..22753
                     /locus_tag="VQ526_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2554542.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            22772..23341
                     /locus_tag="VQ526_000799"
     CDS             22772..23341
                     /locus_tag="VQ526_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2554543.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            23328..24050
                     /locus_tag="VQ526_000800"
     CDS             23328..24050
                     /locus_tag="VQ526_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2554544.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLIIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            24050..24292
                     /gene="yidD"
                     /locus_tag="VQ526_000801"
     CDS             24050..24292
                     /gene="yidD"
                     /locus_tag="VQ526_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2554545.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(24517..25482)
                     /locus_tag="VQ526_000802"
     CDS             complement(24517..25482)
                     /locus_tag="VQ526_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2554546.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(25578..26330)
                     /locus_tag="VQ526_000803"
     CDS             complement(25578..26330)
                     /locus_tag="VQ526_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2554547.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            complement(26327..27283)
                     /locus_tag="VQ526_000804"
     CDS             complement(26327..27283)
                     /locus_tag="VQ526_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2554548.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(27273..28232)
                     /locus_tag="VQ526_000805"
     CDS             complement(27273..28232)
                     /locus_tag="VQ526_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554549.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(28655..29356)
                     /gene="galE"
                     /locus_tag="VQ526_000806"
     CDS             complement(28655..29356)
                     /gene="galE"
                     /locus_tag="VQ526_000806"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2554550.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            29757..30821
                     /gene="pepA"
                     /locus_tag="VQ526_000807"
     CDS             29757..30821
                     /gene="pepA"
                     /locus_tag="VQ526_000807"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2554551.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKDEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            30843..31139
                     /locus_tag="VQ526_000808"
     CDS             30843..31139
                     /locus_tag="VQ526_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2554552.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            31821..32264
                     /locus_tag="VQ526_000809"
     CDS             31821..32264
                     /locus_tag="VQ526_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2554553.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            32268..33032
                     /locus_tag="VQ526_000810"
     CDS             32268..33032
                     /locus_tag="VQ526_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2554554.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            33047..33775
                     /locus_tag="VQ526_000811"
     CDS             33047..33775
                     /locus_tag="VQ526_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554555.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            33768..35288
                     /locus_tag="VQ526_000812"
     CDS             33768..35288
                     /locus_tag="VQ526_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2554556.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
BASE COUNT        10222 a         6575 c         8032 g        10472 t
ORIGIN      
        1 agaagagctc atggaatcta tagtctttga cacagaggaa gagtttagct tgttgataat
       61 tctatgcaga aaacaagaaa gtagtacttt gtttatcaca gtctatatta gtacgagttt
      121 gatgataaat agagatttaa aatgaatttt atttatgaag ccagatcata tgagacttgg
      181 cttcaattag aaaaaggaga gtaatgatgc caattcaaaa taaaaccatg ttgattacct
      241 attctgatag ccttggaaat aatcttaaag acttatatga taatttggaa gagcattttg
      301 gagatgctat tggaggagtt caccttttac catttttccc atcaacaggt gatcgtggat
      361 ttgcgccagt tgactacgac gaagtggact cagcttttgg tgattgggag gatgttaagc
      421 atttaggtga gaaatattat cttatgtttg attttatgat taatcatatt tctcgtcaat
      481 ccaagtatta taaggactat caagaaaaac atgaagccag tgaatttaaa gctctctttt
      541 taaactggga taagttttgg ccagaaaacc gtccgacaca gtctgatgta gatttaattt
      601 acaagcgtaa ggatcgtgca ccaaagcaag agattgtgtt tgaagatggt tcagtggaac
      661 atttgtggaa tacctttggt gaggagcaga ttgatcttga tgtgaccaaa gaagtaacta
      721 tggaatttat ccgtaagacc attcagcact tggcaagtaa tgggtgtgat ttgattcgtc
      781 tagatgcctt tgcttatgca gtgaagaaat tggatactaa tgatttcttt gtggaaccag
      841 atatttggga tttattggac aaagttcgag atatcgctgc tgagtatggg acagagcttt
      901 tacctgagat tcatgaacac tattcgattc agtttaaaat agcagaccat gattactatg
      961 tttatgattt tgctcttcca atggtgacac tttatactct ttacagttcc agaacagagc
     1021 gtttggctaa gtggttaaag atgagcccga tgaagcaatt tacgacgcta gatacccatg
     1081 atgggattgg agtagtagat gtcaaggata tcctgaccga tgaggagatt gactatgctt
     1141 caaatgaact ctataaggtt ggagccaatg tcaaacgtaa gtactctagt gccgagtata
     1201 acaacttaga tatctaccaa atcaattcaa cctactattc agcgcttgga gatgatgatg
     1261 tcaagtattt tctcgctcgt ctaattcaag cttttgcccc aggtattcct caggtttact
     1321 atgtgggtct attagcaggc aagaatgact tgaaattatt agaagaaact aaagaaggtc
     1381 gaaatattaa tcgtcattac tatagcaacg aggaaatagc aaaagaagtg caacgacctg
     1441 ttgtgaaggc ccttctcaat ctattttctt tccgtaaccg ttcagaagcc tttgatctag
     1501 aagggactac tgagatagag acaccaacag ctcacagcat tgtaatcaaa cgtcaaaata
     1561 aagataagtc cgtaacagca gtagcagaaa ttgatttgca aaatcagact tatcgggtaa
     1621 ttgagaatgg agttgaagta acattttgaa gccttgatat ggtggataaa atagggtttt
     1681 tattttggaa aacgtttcct ttgttttcaa attgctaaaa aagtggtaca atataggaca
     1741 gcttactatt atctgaatca gctgatttgg agagaaagga ttcattttga aatcaatagg
     1801 ctttattgaa aagctgaagg ggttgtctag taaagagctg attttattgg gaattatcct
     1861 aagtatcttt ttaccctttt atctttttgt agttgtactc tgtttatata ttatcagttt
     1921 gatttttaca ggagacatga aaagtattct tcagaaaatg ggggagcatc cgatgctgct
     1981 tctttttctt agctatagta ctgttatatc cattcttgca caaaattgga tgggtcttgt
     2041 ggcttcagta ggaatgtttc tatttactat tttctttttg cactatcagt cgattttatc
     2101 ccataaattc tttcgattga ttttgcagtt cgtcttgttt ggtagtgtct tgtcagctgc
     2161 ttttgccagt ttagaacatt tccaaattgt gaagaaattt aactatgctt ttctttcacc
     2221 caatatgcag gtgtggcatc agaaccgggc agaagtgacc ttctttaatc ctaattatta
     2281 tggaattatt tgttgtttct gtattatgat tgctttctat ctgtttacaa cgaccaagtt
     2341 gaattggttg aaagtattct gtgtgattgc aggctttgtt aatctctttg gtttgaactt
     2401 tactcaaaat cgaactgcct ttcctgctat tatcgctgga gcaattatct atctctttac
     2461 gactattaaa aactggaagg ccttttggct tagtattggg gtcttcgcga ttggtttgag
     2521 tttcctcttt tctagtgatt tgggagttcg aatgggtact ttagactctt ctatggaaga
     2581 acgcatttct atctgggatg ctgggatggc cttgtttaag caaaatcctt tttggggtga
     2641 agggccattg acctatatgc actcttatcc tcggatacat gctccttatc atgaacatgc
     2701 ccacagtctt tatattgata cgattctgag ttacggaatt gtgggtacca ttttattagt
     2761 tttgtcttct gttgctcctg ttcgcttgat gatggatatg agtcaggagt cggggaaacg
     2821 tccgattatc ggcctttatc tatctttcct tacagtggtt gctgtgcacg gaatttttga
     2881 cttggctctc ttctggattc agtcaggctt tattttcttg ctagttatgt gcagtattcc
     2941 attggagcat cgaatgttgg tatcggacat gacggattaa gttttataag tttgaaatct
     3001 tctaccttgg gtaggagatt tttttatacg atgggcatgt cgtgcatctg aggtgtaagt
     3061 cctctgtagg gatccactac cggtgaatac aatagcgctc cccaagcctg actatcgtga
     3121 ggtagcggat taaaatggtc tggggataga tcgttttaag tctgacgcag gaaataagaa
     3181 ttgtcagaga aagcaatagt gaaatcgtag gctatgaata gaaacgtgat agcgaggagc
     3241 atatagtgga taaggtagcg agaaatttta aagtataaaa gatagtctta atctatatat
     3301 gtaaatcaca aaagtaattg aattcggaat aaggttggtg tgaaaagaca tagtaaactg
     3361 aatcttgaat actagaatga tacttctaca atattgttat aatttaattt gaattccaaa
     3421 atcacattgt ttcgtccgta tttcatttta ctgtatgtct tcctaaactc caaagtttct
     3481 gcgtcaaatt tcctattttc actgtagtct ttgacacttc gaacgacaat ttgaatcata
     3541 tattcaaaaa aaaatgaaaa atttgtgcca acaagcctaa atattctgaa aatttgtttg
     3601 atttttaatg gataatgtga tataatggtt gtcaatctat aattcacata aacagaggag
     3661 tagaaaatga aaaaaaatcg tgtatttgct acagcaggtc ttgttttatt agcagcaggt
     3721 gtacttgcag catgcagttc ttcaaaatca tctgattcat cagcccctaa agcttatggc
     3781 tatgtttata cagcagaccc agaaaccttg gactacctga tttcaagtaa aaatagtaca
     3841 acagtagtga cttcaaatgg gattgatggt ttattcacta acgataatta cggtaatctt
     3901 gctcctgcag ttgcagagga ttgggaagtc tctaaggatg gtttgaccta cacttataag
     3961 attcgtaaag gggttaaatg gtttacctct gatggagaag aatatgcaga ggtgacggct
     4021 aaagatttcg tgaacggttt aaaacacgca gcagataaaa aatcagaagc tatgtattta
     4081 gctgaaaatt cggttaaagg cttggcagat tatctatcag gaacttcaac agatttttca
     4141 acagttggtg tcaaggcggt tgatgattat acgttacaat acactttgaa ccagcctgaa
     4201 ccgttctgga actctaagtt gacctattct attttctggc ctctgaatga agaattcgaa
     4261 acatcaaaag gaagcgattt tgctaaacca acagatccga catccttgct ttataatggt
     4321 ccattcttgt tgaaagggtt gactgcaaaa tcttctgtag agtttgtaaa aaatgagcaa
     4381 tattgggata aagaaaatgt ccacctagat actatcaatc tagcttacta tgatggatca
     4441 gatcaggagt cgctagagcg taacttcact agtggagctt atagttatgc ccgtctttac
     4501 cctaccagct ccaactattc taaggttgca gaagaataca aggacaatat ctattacaca
     4561 caatcaggct ctgggattgc tggtctgggt gtgaatattg atcgccaaag ttacaactat
     4621 acttctaaaa ctacagattc agagaaagta gctactaaga aggcattgct taacaaagat
     4681 ttccgtcaag ccttgaattt tgctcttgat cgctcagctt actcagctca aatcaatggt
     4741 aaagatggag cagctttagc agttcgtaat ttatttgtaa aaccagactt tgtttcagct
     4801 ggtgagaaga cctttggtga tttagtcgct gctcaacttc ctgcttatgg tgatgagtgg
     4861 aaaggtgtga atttagctga tgggcaggat ggtttattca atgctgacaa ggccaaggca
     4921 gagtttgcga aagctaagaa agctttagaa gcagacggcg ttcagtttcc tattcatctg
     4981 gacgttccag tagaccaagc atcaaaaaac tacatatctc gtattcagtc ctttaaacaa
     5041 tctgtagaaa cagttcttgg tgttgaaaat gtcgttgttg atattcaaca aatgacaagt
     5101 gatgaattcc ttaatattac ttactatgct gccaatgctt catctgagga ttgggatgta
     5161 tcaggaggag tttcatgggg gccagactat caagacccat ctacttacct ggatatttta
     5221 aaaacaacta gcagtgaaac tacaaaaaca tatttaggat ttgataatcc aaatagccct
     5281 tcagtagttc aagttggttt gaaagaatac gataaattag ttgatgaagc tgccagagag
     5341 acaagcgact tgaatgtccg ttatgaaaaa tatgcagcgg ctcaagcatg gttgacagat
     5401 agttcactct ttattcctgc tatggcttct tctggtgcag caccagtgct ttcacgaatt
     5461 gttccattta ctggagcttc tgcgcaaaca ggctctaagg ggtcagatgt ttacttcaaa
     5521 tatttgaaat tacaagataa agtggtgact aaggaagagt atgaaaaagc tcgtgaaaaa
     5581 tggttgaaag aaaaagctga atcaaatgag aaagctcaaa aagaattggc aagtcatgtg
     5641 aagtaaataa tttctaatag aactttctct ccatgaggag aaggttcttt tgggattttt
     5701 aaaggaaata atatgaaaaa atatattttt atgcgtgttt tgcggtcatt ggtttcgatt
     5761 ttcttagtaa cgactttgac ctacacgatt atctatacct tggttcctcg aaaattgatt
     5821 ttcaagcagg atcctaacta taataaaatt gcgacaacgg ctgataaacg tgataactat
     5881 gaaaatactg tgtttgagcg tatgggctac attgagtatt acgatactaa agagttgcaa
     5941 gaaaaggcaa gtagcatgga ttcttctgta acagtagaag caaatgcgac caataaagct
     6001 atttatgaaa agtacatcaa tcaattaggt catggttgga ctttgggaga atttactgaa
     6061 agtggtcaat tctatgctac tcgtgaaatt ccaatttttg aacgtgtttt tcacttctat
     6121 gctaacttga ttgacattga ccatacaaat aaaatccaag accctgaaaa tccagacttg
     6181 aaacgctacc ttcgttttga aaatgatcca gctatcggat ggtcattggt cggttcagga
     6241 actaaacata aatatctctt gtactttaac agtcagttcc catttgttca tcaaaacttt
     6301 gtgaacttga atttaggtga ctcttaccca acctatgcta atacaccagt tcttcaggtt
     6361 attactcaag gtcaaggaca aaccaaaact gcccaagttc agttcccaac aggtaagaaa
     6421 acgtcttctg taaatattta ctcaagaacc tacaagtcac ctagtcaggc tgactctcgt
     6481 gaagtagcta gctatgggaa agatgatcct tatacagcga ctgaaagtaa ttaccaatat
     6541 ccatctatga ttgtcagctc tgctattact ggtttgattg gtttggttct tgcctatgct
     6601 cttgccgtgc cacttggttc agccatggct cgtttcaaga acacttggat tgatagcctc
     6661 tcaacagggg ctttgacctt cttgcttgct cttccaacga ttgccttggt ttacatcgtt
     6721 cgattgattg gatcatctat tgcccttcca gattcattcc ctatcttggg agctggagat
     6781 tggcgttctt acgttttacc agcagtcatc cttggtttgt tgggtgctcc tggtacagcc
     6841 atttggattc gtcgttacat gattgacttg caatctcaag actttgttcg tttcgctcgt
     6901 gcaaaaggtt tgtctgaaaa agaaatttca aacaaacaca tctttaaaaa tgccatggtt
     6961 ccgctggttt caggaattcc tggtgccatt attggggtta tcggtggtgc aacccttact
     7021 gaaacagtct tcgccttccc aggtatgggt aaaatgttga ttgactctgt aaaagcatct
     7081 aataactcta tggtcgttgg tcttgtcttc atctttacat gtatttctat cttctcacgt
     7141 cttttgggag atatttggat gactattatt gacccacgta ttaaattgac tgagaaagga
     7201 ggcaaataat gtctacaatc gataaagaaa aatttcagtt tgtaaaacgt gacgattttg
     7261 cctctgaaac tattgatgcg ccagcatatt cttactggaa atcagtgttt aaacaattta
     7321 tgaagaaaaa atcaactgta gtcatgttgg gaatcttggt agccatcatt ttgataagtt
     7381 tcatctaccc aatgttttct aagtttgatt tcaatgatgt cagcaaggta aacgacttta
     7441 gtgttcgtta tatcaagcca aatgcggagc attggttcgg tactgacagt aacggtaaat
     7501 cgctctttga cggtgtctgg ttcggagctc gtaactccat cctcatttct gtgattgcga
     7561 cagtgattaa cttggttatc ggtgtttttg tcggtggtat ttggggtatt tcaaaatcag
     7621 ttgaccgtgt catgatggaa gtttacaacg tcatctcaaa catcccacct cttttgattg
     7681 ttattgtctt gacttactca atcggagctg gattctggaa tctgattttt gccatgagcg
     7741 taacaacatg gattggtatt gccttcatga tccgtgtgca aatcttgcgc tatcgtgact
     7801 tggaatacaa cttggcgtca cgtactttgg gaacaccaac cttgaagatt gttgccaaaa
     7861 atatcatgcc tcaattggta tctgttattg tgacaaccat gactcaaatg cttccaagct
     7921 ttatctcata cgaagccttc ttgtctttct tcggtcttgg attaccgatt acagtgccaa
     7981 gtttgggtcg tttgatttcg gattattcac aaaacgtaac aaccaatgct tacttgttct
     8041 ggattccatt gacaaccctt gtcttggtat ccttgtccct tttcgtagtt ggtcaaaact
     8101 tagcggatgc tagtgatcca cgtacacata gataggagta gaaatgacaa aagaaaaaaa
     8161 tgtaattttg actgctcgcg atattgtcgt ggaatttgac gttcgtgaca aagtattgac
     8221 agccattcgc ggcgtttccc ttgaactagt cgaaggagaa gtattagcct tggtaggtga
     8281 gtcaggatca ggtaaatctg ttttgacaaa gaccttcaca ggtatgctcg aagaaaatgg
     8341 tcgcattgcc caaggtagta ttgactaccg tggtcaggac ttgacagctt tatcttctca
     8401 caaggattgg gaacaaattc gtggtgctaa gattgcgact atcttccagg acccaatgac
     8461 tagtttggac cccattaaaa caattggtag tcagattaca gaagttattg taaaacacca
     8521 aggaaaaaca gctaaagaag cgaaagaatt ggccattgac tacatgaata aggttggcat
     8581 tccagacgca gatagacgtt ttaatgaata cccattccaa tattctggag gaatgcgtca
     8641 acgtatcgtt attgcgattg cccttgcctg ccgacctgat gtcttgatct gtgatgagcc
     8701 aacaactgcc ttggatgtaa ctattcaagc tcagattatt gatttgctaa aatctttaca
     8761 aaacgagtat catttcacaa caatctttat tacccacgac cttggtgtgg tggcaagtat
     8821 tgcggataag gtagcggtta tgtatgcagg agaaatcgtt gagtatggaa cggttgagga
     8881 agtcttctat gaccctcgcc atccatatac atggagtctc ttgtctagct tgcctcagct
     8941 tgctgatgat aaaggggatc tttactcaat cccaggaaca cctccgtcac tttatactga
     9001 cctgaaaggg gatgcttttg ccttgcgttc tgactacgca atgcagattg acttcgaaca
     9061 aaaagctcct caattctcag tatcagagac acattgggct aaaacttggc ttcttcatga
     9121 ggatgctcca aaagtagaaa aaccagctgt gattgcaaat ctccatgata agatccgtga
     9181 aaaaatggga tttgcccatc tggctgacta ggaggaagga aatgtctgaa aaattagtag
     9241 aaatcaaaga tttagaaatt tccttcggtg aaggaagtaa gaagtttgtc gcggttaaaa
     9301 atgctaactt ctttatcaac aagggagaaa ctttctcgct tgtaggtgag tccggtagtg
     9361 ggaaaacaac tattggtcgt gctatcatcg gtctaaatga tacaagtaat ggagatatca
     9421 tttttgatgg tcaaaagatt aatggtaaga aatcgcgtga acaagctgcg gaattgattc
     9481 gtcgaatcca gatgattttc caagaccctg ccgcaagttt gaatgaacgt gcgactgttg
     9541 attatattat ttctgaaggt ctttacaatc accgtttatt taaggatgaa gaagaacgta
     9601 aagagaaagt tcaaagtatt atccgtgaag taggtcttct tgctgagcac ttgactcgtt
     9661 accctcatga attctcaggc ggtcaacgtc aacgtatcgg tattgcccgt gccttggtca
     9721 tgcaaccaga ctttgttatt gcagatgagc caatttcagc cttggacgtt tctgtacgtg
     9781 cccaagtctt gaacttgctc aaaaaattcc aaaaagagct cggcttgacc tatctcttca
     9841 tcgctcatga cttgtcggtt gttcgcttta tttcagatcg tatcgcagtt atttacaagg
     9901 gtgttattgt agaggttgca gaaacagaag aattgtttaa caatccaatt cacccatata
     9961 ctcaagcctt gctttcagca gtaccaatcc cagatccaat cttggaacgt aagaaggtct
    10021 tgaaggttta cgacccaagt caacacgact atgagactga taagccatct atggtagaaa
    10081 tccgtccagg tcactatgtt tgggcgaacc aagccgaatt ggcacgttat caaaaaggac
    10141 taaactaata atggttttat aatttccatg tcaattttta tgggaattat aaaacttttt
    10201 ttgttggact gatttgaaaa actttgaaag cttcctgaaa gaattttcga aaaattttaa
    10261 aaaattttga aatattattg acaggtgatg aaaaaggtgg taggatagga aggatcaaaa
    10321 aagaaagcag aaaaagaaat gaataagaga caagccgtaa ctatgaatca aaacttttac
    10381 tttagctact ttagatagtg tttgtagaca tgtcaccatg gatgcaaaca gttcaagcaa
    10441 tttgtttgta tctatgtggc taagattttt gattgtggtc catatagatg aatatctaaa
    10501 tggactagct aagttgtaac ttgttaggtt atttcaagta cccgtttaga tagtatctag
    10561 gcggtttttt gttttatctt ttctgagaag gagtttcatt atgaaagttg ttcgaaaatc
    10621 actagccccc tcttggtagt ggggatcctc ttgacttctc tgatcagttt gcatcagttg
    10681 aaggcagata agaaaagaga tgtctttcgt atcggtattt cgcagtttat tacccaccag
    10741 tccttagacg ctactagaga aggatttgtg gatgagctgg ccaaacaagg ctatgttgag
    10801 ggggaaaata ttgagattga tttgcaaaat gcacagggag aacaaagaaa tctaaaaacg
    10861 atttcccagc aactagcaga atccagcgat gtcgttctag ccatcgcaag gccttctgct
    10921 cagagcttgg ccaatacgac gcaaacgaca ccagttatct tttcagctgt aacagaccca
    10981 gtcagtgcca agttggttga gtcaagagaa catcctgggg gcaatgtaac tgggacaagc
    11041 gatcagtcat cagatgccat ttcaacccaa atcaatttga taaagaaagt gttgccaaag
    11101 gctaaaacga ttggaatcct ctatactcag agcgagccca attcggttgt ttaaaatgat
    11161 gaagctaagc gacttctgaa agaaaaaggc tttaccgttg ttgaaaaaac aatcttggac
    11221 agtaataacg tcaaggcggc agcagagagt ttgatggcag aggtggatat ggtttttgtc
    11281 ccaactgata atatcatttc gtcaaccatg gaaacagtta agcaggtttc tattaaacac
    11341 aaggttccag tattcggtgg ttcaacagaa atgattgctg ttggtgactt gtataactac
    11401 ggaaccaatt atgaagaatt gggaagacag acagctcgca tgctgattcg tgttttaaaa
    11461 ggtgagaaac cagaaaatat agcagttgag ttgcctgaaa aactggaatt gcataccaat
    11521 caagaaatgg cagatgcatt gggaattgat attagtaagt tagaaggcaa ggaataagga
    11581 ggattaagat gaattttgtg ttatctagtt tatcagaagg tttgttgtgg tcgattgtgg
    11641 cgattggggt ctacttgact ttccgtattt tggatattgc ggatataact gctgagggag
    11701 cctttcctct gggggcagca gtcgttgtat cacagataca ggcaggggca aatccttggc
    11761 ttgcgacttt acttgctttg ctggcaggta tggtagcagg tcttgtatca ggaatgctcc
    11821 acaccaagat gaaaatccca gctctcttga cagggattgt gaccttgaca gggctctatt
    11881 caatcaatat taaaattatg ggcagtgtac ccaatctttc cttgggagat tctgcaaccg
    11941 tctttaaaca gttggcgagc ttgggtctga caaatgaagg agctgttttc tcacttagtt
    12001 tagtctgtct cttacttgtt tggtcttgac ccttttgatg aaaacagaga ttggtttggt
    12061 cttgcgttcg actggggata atatcccgat aagtgaggcc aatggagtca atgtagacac
    12121 catgaagatt gttggttaca tgatttcaaa cggtttgatt gccctatgtg gttccttgtt
    12181 tgcccaaaat gatggatttt cagatgtgac ttctgggaca ggaaccattg ttgttggttt
    12241 gagctcggaa gtcttgatac acgacttgac cattggaggc cgcttattat ccatcggaat
    12301 cggtgctatt gtttaccgtt tgattatttt aaatatctat gaaattccaa atctagatca
    12361 aaacttggta cgtctcttta atgcaatctt gctcgctttg gttttatttg caccagaatt
    12421 gcaaaagaga ttaaagattc gtggtctgaa attgagaaat gaataggagg agaaagttat
    12481 ggcaagtttg ttaacacttg aaaatattca caagacattt gaagcaggga cggtcaatga
    12541 gaaccatgtc ctcaaaggat tagatttaga ggttgaagag ggagatttta tctatgtgat
    12601 tggtggaaat ggagcaggaa aatccacttt gatgaatatc ttggctggca atctatcggt
    12661 ggacgaaggg gaccttctac tggcagggaa atccattaaa aatctgagtg tcagaaagag
    12721 agccaaggat attgcccgtg tttttcaata tcctaagatg ggaacagctt ctcgtttgac
    12781 gattgaggag aatatggcta ttgccttgag acgtgggcaa aagagagggc ttggttgggg
    12841 tgtgaaggag aaggatagaa tccagttcca agaggccttg aaggagttga atattggtct
    12901 tgaaaaccgc ttaaaagtag atactcaata tctctcagga ggacaaagac aggctttgac
    12961 cctagtcatg gcagctctgg tgaaacccaa acttttactt ttggatgaac atactgcggc
    13021 acttgacccg aaaaccagtc aaatggtgat ggacttgacg caaaagattg tggagcacca
    13081 tcagtggacg actttgatga ttacccatga tatgaatcat gcgattgagt acggcaatcg
    13141 tctcattatg ctctatcaag gcaagatagt ggtggatgtc aagggagaag agaaaaagca
    13201 tctgacagtt gaagacctta tgcatctctt ccagaaaaat agtggccaaa gtctggtcag
    13261 tgatgaatta gttttgggat aaagaaaaag gctgagatct ggtgtctcag tcttttattt
    13321 cttgcgtttt gcaaaatcta caaatctaaa tttttctaac ttatggcgac tttcggtaaa
    13381 ctgaaattgg cgtccgtctt ggagatagac ttttgattta atcgaaacga cataagggtc
    13441 tttgccaatg tccatgagaa tcttgtctcg gtcgcttgag tggtcaatgg tgatttcctt
    13501 ctgagcataa tcaataagga gtttgaggcc attttctata taagaataga tagactgctc
    13561 agcaatttgg cgagtgagat ttggaattag ttccatatcc agatagtctg tatccagaac
    13621 ggataccaga tcatccacca cacgctggcg gaccaccttc caaaccatcc gaaactctgg
    13681 gaaaccggtt atcagtgagg attttttatc aataataatc ttgtccagac tgaccacgtt
    13741 ggttttagag cgcagtccaa gttctttaac tagttcttgg tagctggtta ggttggatac
    13801 agggaaattg acggtttctt ctttgacgac ttgagaacct tgccctctta tctttttgat
    13861 caatccttcc tcttggagga gagacagggc ctttcggacg gtatcacgac tcacttgata
    13921 ttgctccata aggtcgtgct cgctaggaag gaaatctccg acagcgtaag tctcgttttg
    13981 aatggtttct tggatttgct taaataattg ttggtatttc ttcattttgc ctgcttaaat
    14041 aggtttttag tctagtttaa tgaacacctg tatctatttt accatacaag tgctagaaaa
    14101 tttacaaaaa aacacccttt ttataccaaa caagttgcag acaagtttgg tatcgtttac
    14161 aatatactta tcaaatcaaa cttgctttga caagtataag gagaatcaaa tgggaaaatt
    14221 tgaacaagaa gccaaagatc tgcttcaggc aattggaggc aaagaaaatg tgactgccgt
    14281 aactcactgt gcgacacgga tgcgctttgt tttaggagat gataagaagg ctaatgttaa
    14341 agctatcgag tcaattccag ctgttaaagg aacttttaca aatgcaggtc aatttcaggt
    14401 aatcattgga aatgacgtgc ccatctttta taatgatttt acagccgttt caggtattga
    14461 gggtgtttcc aaagaagcag ccaagtctgc agctaagagt aatcaaaacg tggtccaacg
    14521 tgttatgacc actctggcgg agatttttac tccgattatt ccagccttga tagtcggagg
    14581 attgatcctc ggtttccgta atgtcttgga aggtgtccat tggtcgatgt tggatggcaa
    14641 gaccatcaca gaatcctctc agttttgggc aggtgtcaat cacttcctct ggttgcctgg
    14701 tgaagctatc ttccagttct taccagtagg gattacttgg tctgtttctc gtaagatggg
    14761 aaccagccaa attttgggaa ttgttctcgg aatctgtttg gtatcgcctc agttgctcaa
    14821 tgcctatgcg gttgcttcaa cgccagcagc tgatatcgcg gcaaactggg tttggaattt
    14881 tggctatttt actgttaatc gtatcggtta ccaagcccaa gttatcccag ccttgcttgc
    14941 aggtttgagt ctgtcttatc ttgaaatctt ctggcgcaag catatcccag aagtcatttc
    15001 tatgattttt gtacctttct tgtcattgat tccagccttg attttggctc atactgtttt
    15061 gggaccaatc ggttggacaa ttggacaagg gctttcatca gttgtcttgg caggtttaac
    15121 tggtccagtt aaatggctct tcggtgcaat ttttggtgcc ctctacgctc catttgtcat
    15181 cacaggtctg caccatatga ccaatgccat tgatacacaa ttgattgcgg atgctggtgg
    15241 cactgcccta tggccaatga ttgctctttc taatattgct caaggctcag ccgtgtttgc
    15301 ctattatttc atgcatcgcc atgatgagcg tgaggctcag gtttcacttc ctgcaaccat
    15361 ttcagcctat ctcggtgtta cagaaccagc tctttttggg gttaacgtaa aatatattta
    15421 tccatttgtt gctgggatga ctggttcagc ccttgcaggc atgttatccg ttacttttaa
    15481 tgtaactgcg gcttctattg gtatcggtgg tttgccaggt attctctcta ttcaacctca
    15541 atacatgctg ccatttgcag gaactatgct agttgcgatt gttgttccaa tgctcttgac
    15601 tttcttcttc cgcaaggctg gtctctttac aaaaacagag ggcgatacga acttgcaggc
    15661 agaattcgtt gctcaagaag aagcagaatt tgtgaaccat gaaccagtag aacttacttc
    15721 ggtagaaatt atcagcccac taactggcca agtgaaagaa ttgagtcaag cgacggatcc
    15781 tatttttgca tcaggtgtca tggggcaagg tctagtcatt gaaccaagcc aaggtgagtt
    15841 gacctctcca gttaatggga cagtgacggt tcttttccct accaagcatg ccatcggcat
    15901 tgtctctgac gagggagttg aattgctcat ccacatcggt atggatacag taggtcttga
    15961 tggcaaaggt tttgaaagtc ttgtagtcca aggagatcac gttacagttg gtcagcaact
    16021 gattcgtttt gatatggatg tcattaaggc tgcaggtctg gtgacagaaa ctcctgttat
    16081 catcaccaac caagatgctt atacagcgac tattcccgga acttatccga caacgatcca
    16141 agctggagca tctctcatgg tcgctacacg aatctaatac tcttcgaaaa tctcttcaaa
    16201 ccacgtcagc tctatctgca acctcaaaat agtgttttga gcaacctgtg gctagcttcc
    16261 tagtttgttc tttgattttc attgagtata attgaaatag cacaaatagg gacggaagac
    16321 tgcttcctcc cttttatcag aaaaggagaa acagatgaca cttgataaag gaaaagtagt
    16381 ctatcaaatc tatccaaaat cttacaaaga caccactgaa aacggtattg gggatttccg
    16441 tgggattatc ggaaaaattc cctatctagc caaactaggt gtggatatgg tctggctcaa
    16501 tccattctat ccaagtcctc aacgggataa tggttacgat atttcagatt atatggcagt
    16561 ggatcctctt tttggtgata tggctgattt tgaggaaatg gtgtgtatcg gtaaagagca
    16621 caagattgac tttatgctgg acatggtact taatcattgt tcgacagagc atgaatggtt
    16681 tcagaaagcc ctagctggtg ataagtatta tcaagacttt ttcttcatcc aagaccaacc
    16741 aacagattgg cagtctaagt ttggtggttc tgcatgggcg cctttcgggg atactgggaa
    16801 atactatctt cacctatttg atgagaccca ggctgacctt aactggcgca atcccaatgt
    16861 ccgtaaggag cttttcaagg ttgttaattt ctggcgcgac aagggtgtca aaggttttcg
    16921 atttgatgtg atcaatttga ttggtaagga cgaggtttca gtggactgtc ctgaaaatga
    16981 agggaagcca gcttatacag acaaacccat cgttcataac tatttgcgta tgatgaacca
    17041 agccactttt ggatctgacg atagctttat gacagttggg gaaatgtctt ctaccaccat
    17101 ggaaaactgt gtcctctatt cgtcgcctga tcgtcaggaa ttatccatga cctttaattt
    17161 tcatcacctc aaggtggact ataaggatgg acaaaagtgg accttggctc cctttgattt
    17221 tgaagagttg aagagtcttt accatagttg gggtaaggaa atgagtgata aagacggttg
    17281 gagtgccctc ttttggaaca atcacgatca accacgtgcc ttgaaccgtt ttgtcgatat
    17341 tcaaaacttc cgcaaggaag gagccaccat gctagcagcc agcattcacc tgtcacgtgg
    17401 gacaccttat atctacatgg gtgaggaaat cgggatgatt gacccagact atgattctat
    17461 ggctgattat gtggatgtcg aatcgcttaa tgcctatcag atgctcttag aagaaggaaa
    17521 gagtcagcaa gaagccttcc agattattca agctaagtcg cgtgataatt cacgcattcc
    17581 catgcagtgg gatgcttcgg aaaatgcagg attttcaaca ggcactcctt ggttgaaagc
    17641 tggtaaatcc tataaataca tcaatgtaga aaatgaaatc cagggtccga tttttacctt
    17701 ctatcaagac ttgattcgac tgcgtaagga aatgccaatc atttcagaag gaagttacaa
    17761 accagccttt gaagatagca aacaagtcta cgcttttgaa cgccagtttg aggatcaaaa
    17821 gttactagtg ctcaataact tttatgctaa agaagtggaa atcgacttgc cagcagtcta
    17881 ccaaaatgga cagattttga tttcaaacta tgaagatgca gaagtgtctg agaaaattct
    17941 actaaaaccc tatcaaacac tggctattta tgtaaattaa acttagcaag gagacctctt
    18001 tttcagaaaa gcagggttct ttttgctgtc tattccatcc ttgaaagtgt agaacaaact
    18061 ggtcagggtg gctagtttgt ggtataatga aagggactca aaaagaaaca ggagaaaaat
    18121 gatggattta cttttagcaa ttgtattgat tgtgctagct tttctaggag gagctcttgg
    18181 aggaatgtac ttggttcgta agcaaatcga aaaagaattt gctgacaacc cacgtttgaa
    18241 tgctgaagca gttcgtactc ttttgagtgc aaatggtcaa aaaccaagcg aagctaaggt
    18301 acaacaagtt taccaccaaa tcatccgcca acaaaaggca gcccttgcta acaataaaaa
    18361 gaaaaaataa ataaggaaaa gtctgggatg aaagttccag acttctcact atgttggtta
    18421 tggtttaggg atgagacttt ttccttgcat tctattgata tttgattatg attaagagag
    18481 aggtagttga cattctatca agctgtcggt cagttcgttc agtacaagga atcataatga
    18541 acgatcaatc agaaaaaaga ctagaaagaa gactgtatgg ataatcgacc aattggtttt
    18601 ttggattcgg gtgtcggggg cttgaccgtt gtgcgcgagc tcatgcgcca gcttccccat
    18661 gaagaaatcg tctatattgg agattcggcg cgggcgccct atggcccccg tcctgctgag
    18721 caaattcgtg aatatacttg gcagctggtc aactttctct tgaccaagga tgtcaaaatg
    18781 attgtcattg cttgtaacac tgcgactgcg gtcgtctggg aagaaatcaa ggctcaacta
    18841 gatattcctg tcttgggtgt aattttgcca ggagcttcgg cagccatcaa gtccagtcaa
    18901 ggtgggaaaa tcggagtgat tggaacgccc atgacggtac aatcagacat ataccgtcag
    18961 aaaatccatg atctggatcc cgacttacag gtggagagct tggcctgtcc caagtttgct
    19021 cccttggttg agtcaggtgc cctgtcaacc agtgttacca agaaggtggt ctatgaaacc
    19081 ctgcgtccct tggttggaaa ggtggatagc ctgattttgg gctgtactca ttatccactc
    19141 cttcgcccta ttatccaaaa tgtgatgggg ccaaaggttc agctcatcga tagtggggca
    19201 gagtgcgtac gggatatctc agtcttactc aattattttg aaatcaatcg tggtcgcgat
    19261 gctggaccac tccatcaccg tttttacaca acagccagta gccaaagttt tgcacaaatt
    19321 ggtgaagaat ggctggaaaa agagattcat gtggagcatg tagaattatg acaaataaaa
    19381 tttatgaata taaggatgac caggactggt atgttgggtc ttatagtatt tttggtggcg
    19441 ttaacagttt gagcgactat cagacagatt ttcctctgtt tgaattctcc aaaatatttg
    19501 gagatgaaga gtatggtttc ccgctttcag ttactgtttt acgctatggt tctacctacc
    19561 gtttgttctc ctttgtggta gatatgctta atcaagaaat gggacgaaac ttggaagtta
    19621 ttcaacgtca tggggccctg ctcttggttg aaaatgggca actcttgtat gtagaattgc
    19681 ctaaagaagg ggtcaatgtt catgatttct ttgagacaag caaggtcaga gaaaccttgt
    19741 tgattgcgac tcgtaacgaa ggcaaaacca aggaattccg agctatcttt gataagttag
    19801 gctacgatgt ggaaaatctt aatgactacc ctgacctgcc tgaagtagca gaaacaggta
    19861 tgacctttga agaaaatgcc cgccttaagg cagaaaccat ttctcaatta acgggtaaga
    19921 tggttttggc agatgattct ggtctcaaag tcgatgtcct tggtggctta ccaggcgtct
    19981 ggtcagctcg tttcgcaggt gtgggagcaa ctgaccgtga aaataatgcc aaactcttgc
    20041 acgaattggc catggtcttt gaactcaagg accgctcggc tcagttccac acaaccctag
    20101 tcgtagccag cccaaataag gaaagtttag ttgttgaagc agactggtca ggttatatta
    20161 actttgaacc taagggtgaa aatggctttg gctatgatcc cctcttcctt gtaggagaga
    20221 caggtgagtc atcagctgaa ttaaccctgg aagaaaaaaa tagtcaatct caccgtgcct
    20281 tagccgttaa gaaacttttg gaggtatttc catcatggca aagcaaacca tcattgtaat
    20341 gagcgattcc catggcgata gcttgattgt ggaagaagtc cgtgatcgct atgtgggcaa
    20401 agtcgatgcc gtttttcata acggcgattc tgaactacgt ccggattctc cactttggga
    20461 gggcatccgc gttgttaaag ggaacatgga cttctacgcc ggctacccag aacgtctggt
    20521 gactgagctt ggttcgacca agattatcca aactcatggt cacttgtttg acatcaattt
    20581 caactttcaa aagttggact actgggctca ggaggaagag gccgctatct gcctctatgg
    20641 tcacttgcat gtgccaagtg cttggatgga aggcaagatc ctctttctaa atccaggttc
    20701 tatcagccaa ccacgaggta ccatcagaga atgtctctat gctcgtgtgg agattgatga
    20761 tagttacttc aaagtggact ttttgacacg agatcacgag gtgtatccag gtttgtccaa
    20821 ggagtttagc cgatgattgc caaggagttt gagactttct tgttggggca ggaggaaact
    20881 tttttgaccc ctgctaaaaa tctagctgtg ttgattgata cccacaatgc ggatcatgcg
    20941 accctcttgc ttagtcagat gacctatacc cgtgttcccg ttgtgacaga tgaaaaacag
    21001 tttgttggga cgattggact cagagatatt atggcttatc agatggagca tgacttgagc
    21061 caagaaatca tggcggatac ggatatcgtt catatgacaa aaacggacgt agcggttgtt
    21121 tcgcctgatt tcaccattac ggaggtcttg cacaagctag tagatgagtc cttcttaccg
    21181 gttgtggata cagagggtat tttccaaggg attattacgc gcaagtccat cctcaaggcc
    21241 gttaatgccc tcttgcatga ttttagtaag gaatatgaga ttcgatgcca atgagagaca
    21301 gaatttcagc ctttttagag gaaaagcagg gcttgtctgt caattccaag cagtcctata
    21361 agtatgattt ggagcaattt ttagacatgg taggtgagcg gatttctgag accagtctca
    21421 agatttacca agcccagcta gccaatctaa aaatcagcgc ccagaagcga aagatttcgg
    21481 cctgtaacca atttctatac tttctctatc aaaaaggaga ggtggacagc ttttaccgct
    21541 tggaattagc caaacaagct gaaaagaaga cggaaaagcc agagattcta tacctagact
    21601 ctttttggca ggaaagcgac catccagagg gccgcttgct agcgctctta atcctagaaa
    21661 tggggctctt gcccagtgag attttagcca tcaaggttgc ggacatcaat ctggattttc
    21721 aggtgctgcg aatcagcaag gcttcccaac agaggattgt caccattccc acggccttgc
    21781 tttcagaatt ggaacccttg atggggcaga cctatctttt tgaaagagga gggaaaccct
    21841 attctcgtca gtgggccttt cgtcagttag aatcttttgt caaggagaaa ggttttccat
    21901 ccttatcagc tcaagtctta cgtgaacagt ttattctaag acaaatagaa aacaaggtcg
    21961 atttgtacga aattgcaaaa aaattaggat taaaaacagt cctgacctta gaaaaatata
    22021 gataatggat attaaattaa aagattttga aggacccctg gacttactct tgcatctggt
    22081 ttctaagtac cagatggata tctacgatgt gcccattacg gaagtcatcg aacagtatct
    22141 agcctatgtc tcaaccctgc aggccatgcg tctggaagtg acgggtgagt acatggtcat
    22201 ggctagtcag ctcatgctga ttaagagtcg taaactcctt ccgaaggtag cagaagtgac
    22261 agacttgggg gatgacctgg agcaggacct cctctctcaa atcgaagaat atcgcaagtt
    22321 caagctcttg ggtgagcact tggaagccaa gcaccaagaa cgggcccagt attattccaa
    22381 agcgccgaca gagttgattt acgaagatgc ggagcttgtg catgacaaga cgaccattga
    22441 cctctttttg gctttttcaa atatcctagc caagaaaaaa gaggagtttg cacaaaatca
    22501 cacgacgatc ttgcgggatg agtataagat tgaggacatg atgattatcg tgaaagagtc
    22561 cttgattgga cgagatcaat tgcgcttgca ggatttgttc aaggaagccc agaatgtcca
    22621 agaggtcatc accctctttt tggcaaccct agagttaatc aaaacccagg agctgatcct
    22681 cgtgcaagag gagagttttg gagatatcta tctcatggaa aagaaggaag aaagtcaagt
    22741 gcctcaaagc tagacttgat agagaggaaa gatgagtact ttagcaaaaa tagaagcgct
    22801 cttgtttgta gcgggtgaag atgggattcg ggtccgccag ttagctgaac tcctctctct
    22861 accaccgaca ggcatccagc aaagtttagg aaaattagcc cagaagtatg aaaaggaccc
    22921 agattccagt ttggctttga ttgagacaag tggtgcttat agattggtga ccaagcctca
    22981 atttgcagag attttgaagg aatactctaa ggcgcctatc aaccagagct tgtctcgggc
    23041 tgcccttgag accttgtcca ttattgccta caaacagccg attacgcgga tagaaattga
    23101 tgccatccgt ggagttaact cgagtggagc cttggcaaag ttgcaggctt ttgacctgat
    23161 aaaggaagac gggaaaaagg aagtattggg gcgccccaac ctctatgtga ctacggatta
    23221 tttcctagat tacatgggga taaaccattt agaagaatta ccagtgattg atgagcttga
    23281 gattcaagcc caagaaagcc aattatttgg tgaaaggata gaagaagatg agaatcaata
    23341 agtatattgc ccacgcaggt gtggccagta ggagaaaagc agaagagctg attaagcaag
    23401 gcttggtgac ggttaacggc caagtggtgc gtgaactagc aaccactatc aagtcaggcg
    23461 acaaggtcga agttgaaggt caacctatct acaacgaaga aaaggtctac tatctgctta
    23521 acaaaccacg cggtgtgatt tccagtgtga cagatgataa gggtcgcaag acggttgtcg
    23581 acctcttgcc caatgtcaaa gagcgtattt accctgtggg tcgtttggac tgggatacat
    23641 caggagtctt gattttgacc aatgatgggg actttacaga cgagatgatt caccctcgta
    23701 atgagattga caaggtttat gtcgcgcgtg ttaaaggtgt ggccaataag gacaatctcc
    23761 gccccttgac ccgtggtctt gagattgatg gtaagaaaac caagccagct gtttatgaaa
    23821 ttctcaaagt ggacccagtc aaaaatcgct ctgtggtgca gttgatcatc catgaagggc
    23881 gtaaccatca ggttaaaaag atgtttgaag ctgttggtct ccaagtagat aagttgtctc
    23941 ggactcgttt tggacaccta gacttgacag gactccgtcc aggagaatcc cgtcgtctta
    24001 ataaaaaaga aatcagccaa ctacacacca tggctgtaac taagaaataa tgaaacgaat
    24061 tttaatagcg cctgtgcgct tttaccaacg ttttatctca ccagtctttc caccctcttg
    24121 tcgctttgag ctgacttgtt ccaactacat gattcaggct attgaaaaac atggttttaa
    24181 gggggtattg atgggcttgg ctcggatttt acgttgtcat ccctggtcga aaacaggtaa
    24241 ggaccccgtt ccagaccgct tttcccttaa acgaaatcaa gaaggggaat gaggtggggt
    24301 aaatagattt caaaatgata aaaacgcatc ctatcaggtt tgagtgaact tgataggatg
    24361 cgttttagaa tgtcaaaatt ttatactctt cgaaaatctc ttcaaaccgc gtcagctttc
    24421 atctgcaacc tcaaaacagt gttttgagca acctgcggct agttttctag tttgctcttt
    24481 gattttcatt gagtattaaa ttgagtttga agtggcttat ttcaaagctt tttgtatgtc
    24541 ttcaatcatg agttttgttg attcaagtcc gcctccgctt agataccaga ggtctggtgt
    24601 tagttggata atcttaccat ttttagcagc aggtgtttca gcgataaggg cattttctag
    24661 gacaccgtcg ttgctagagt tgtccccacc gatggcaagg gtacggttga tgacaaagag
    24721 gatgtcaggg ttgatttctt tgacactttc aaagctgact tcttgtccgt ggcgtgagtc
    24781 ttcaaatgtt gtatcagttg gtttgaattt caaggtttgg tacaagaaag agaaacgaga
    24841 tttggcacca aaggctgcca tttttccttc attaaggagg atcgcaaggg cttttttgtc
    24901 agagctttca tttttagtag cgacttcttg gatgctcttg tctagcttgg ccaattcttc
    24961 cttggctttc tgtgtaccag tttcgccgaa ggcacttgct aaggattcga tattagcctt
    25021 ggtagaagtc cagtagtcgt ccttgcttgc ttggaagaga acggttgggg cgatttcttt
    25081 gaatttgtct acgaattttt gtgtacgtgg tgaagcgata atcaaatcag gctcaagggc
    25141 ggcgatagct tctaaatcag gttctttcat agaaccaaca tttttgacag ttcccactag
    25201 gtcttttaga taagtcggaa cagtttttgt aggcattccg acgatatttt tttcaaatcc
    25261 taaagcgcga atagtatccg cagcgccgag gtcaaaggtc acaatctttt caggaacttt
    25321 ggaaagtttg acctcgtcca gtgaactttt aatggttacc tctgttggag cagagctact
    25381 ggtctctgtc tgactagtgc ttgagtttgt actacatgca ccaagtagga gcaagaagct
    25441 ggccactagg gcagtgaaat aaagtttaag ggatgttttc ataatttctc ctttttaaaa
    25501 tgtgataacg atttagggag tctcttaatc ttattgacta agagactgaa ggttctctaa
    25561 cttgagcttt tatgttacta gctatagata cagatctttt tgttattgat atcagctagc
    25621 gtgatgggaa tctcataaag ttgactgagc aggtcagcct gcatgatttg atcggttctt
    25681 cccttgctaa agacctggcc gtccttgaag gcgacaattt catctgcata ctgactggcc
    25741 atgttgatat cgtggaggac gatgataatg gtcttgccga gttcctccac cagtcgtcga
    25801 agaatctgca tcatgctgac gctttgcttg atatcgagat tgttgagtgg ttcgtccagc
    25861 aagataaagt ccgtatcctg ggccagtacc atagcgataa agacgcgctg gagttgcccc
    25921 cctgacaggc tattgatgta gcggtctttt aagttggtca gttccaaata gttcagagtt
    25981 tctcggattt tttcccagtc ttctgatcta agtcgacctc ggctgtaggg aaaacgtcca
    26041 aaactgacca gttcttcaac agtcaatttg gcttggtaat tgattttctg ttttaggatg
    26101 gttagttctt gggccagttc ttgcgaattc cagctctcga tttcacgtcc tttgatactg
    26161 agaactccct gatctttctt ggttagtctg ctcatgatgg agaggagagt cgattttcca
    26221 gcaccatttg gaccaataaa ggctgtcagt ttttgaggac tgacttcaag cgaaatgcct
    26281 tgcaaaatat cctgtttttg aatggatttg tcaatgtttt ccagtttcac tgacgagccc
    26341 tcctatatag taagataaag aataagaagc cacccacact ctcaatgatc atactgatac
    26401 gaatttccag tgcaaagact cgttcaatca aggcttgccc caaggttaag ctaataaatc
    26461 caaccagaat ggccactata aagagtaact tgtgctgata gtctttgaca atcaggtagg
    26521 tgaggttggc cagcataaag ccgaagaagg ccataggtcc taccaaggca gtggccgttg
    26581 aggtcaaaag cacgattccc cagaggagct ctttctgttc tttttcaaca tcgagtccca
    26641 atatctgagc cgtttctctt tgcaggtgca agacatctag aacgactgct tttcgaaaga
    26701 aaaagattgt caaagcgagg atgatcagag aaccgatggc taggatggaa gtgttaagat
    26761 gttgaaagga ggcaaaaaga ctattttgca gtttatcgta ttcgtttgga tccattagga
    26821 cttgaaggaa ggtgctgata tttcgaaaga gacttctgag cgctagacag atcagcagga
    26881 tgaagaccag gtcttgcttc atcagtgtct tcaagtaacc ttgtaaggcg agaaagaaga
    26941 gggactggac aagaagtaag actaggaatt ctaagatagg ggatttgccc agttgaagaa
    27001 acttgctttc aaaaaccagt agtagggttt gtagtaggac gtagaaggat tcaattccca
    27061 aaatactagg cgtcaggaag cgattttccg tcagggtttg aaaactaatg gtcgaaatcc
    27121 cagtcgcgat ggctaccaag agataaacga tgatcttttg ggaacgcaac ttccaagcaa
    27181 aggctgacaa gtgagtgatg ggccaaaagt agagaagaca agctccgatg gcaagaataa
    27241 tgagaatcca gaagagcttg gtatgtttgc ttttagtctg catcttttcg tccccctctc
    27301 cagagaagta ggataaagac gagactaccg atgattccta gcaagagact gacagacaac
    27361 tcatagggcc taatcagaac tcgggatagg atatcgcaag ccagaactag attggcacca
    27421 accagtgcga ccatgagttt ggtttgactt agattatctc catagcgctt gcgaacaaga
    27481 ttgggaacga taactccgag aaatggtaag ccacccacgg taatcatggt gacgcttgtc
    27541 gttagcgcca ccagaaagag ggccagtttt tcaagtaggg agtaggaaat ccccaaactc
    27601 tcgctggttt ctttccccag attcatgatg gtgaaggttt gggataattt ccaaacggtt
    27661 atcaggatga tgaggcctaa gaagagccac tcatactgat gggtctgaat catggagaag
    27721 gagccctggg tccaggcagt catactctga accagattga aacgataggc gataacttct
    27781 gtgactgagc cgataatccc gctatagatg atcccaatca gaggcaacat ccacctttcc
    27841 tttacagtaa aaatggtcat aaaggctagg aagaagaggg tgaatacgat ggatgaaaca
    27901 aaagcgaaga gcatcttttg ggtcagacta gccgatggaa agacaaaaag gctcagcacc
    27961 attcccagtt tggcggcttc agtcgttcca actgtactcg gtgcagcaaa ctgattttgg
    28021 gtaatagtct gcatgagaag gcctgccata ctcatactag aggcagtcag gagaatactg
    28081 atagttcttg ggagacggga ctcttgaaag aggagccagg tctgctggtc gaaatcaaat
    28141 agctttcccc atgaaaaatc actggtccca atgctaatag agagaaagac taggagtaga
    28201 agtaagccaa ttaaataatg agaaagtttc ataccccgtc ctttcatgta gatttggtat
    28261 cgaaagatat ctgcggatat aaatgtaaca ttatttttct aatctgtcaa taaaatttct
    28321 gacaatttaa taaatacaac aaggagagag caacaagact ttctcctttg ttatcctatt
    28381 ctaaaatgtt tttaccttaa tctgataaaa taatatcttc gagggagtag ctagccgtcc
    28441 aatcaagata ttgtttagct tttgaagcat ctgctaggac actggctggg tcactagcac
    28501 gtcgagcaac aatctcgtgt gggatttttt aatttagtaa ttcttcagca gttttaaaga
    28561 tttctttgat agtatagcct tttttagttc ctaagttaaa gatttgagaa gaactgtctt
    28621 cttgaaatag gtagttcatt cctttaacat gagcctatgc aaggtccaag acataaatgt
    28681 aatctcgaat acatgaaccg tcacgtgtat cgtagtcatc tccaaatatt tttaagctat
    28741 catgttgtcc caatgcggtc ttgttgatat ttggaatgat gtgagttgga tttttcacac
    28801 gcagaccgtt tgaagcatcc atttcagccc cagcaacatt aaagtaacgg aaaataacat
    28861 atttccagtc gtagcgattg gccatccagt aaatcattcg ttcgcccatc agttttgtct
    28921 ctgcataagg gttgacaggg tcgagcaggg tatcttcagt caccggcttg tcaatacagt
    28981 tatttccata gagagaagca gtcgaagaga acatgatttt ttgaatgcca acttcagata
    29041 agactttgag aacttggttc ataccagcaa cgttggcagt gaagtattta cttggatttt
    29101 caatactttc gctcacaaca atttcacctg cacaatggag gacagcatca atctgatttt
    29161 cttctaagta agcctttaag gcgctagcat cataaacatc cagttcttta aagctagcac
    29221 gactatccac agccgctcga tttcctgtgg agagattatc gaggacatgc acctgatagc
    29281 cagcatttaa aagagcttta acggtatggg agccaatgta gctagtccca cctgtaacaa
    29341 gaattgtttt ggtcatgatt ttttcctttt ctagttttgt actatagtaa attgaaataa
    29401 gatatgaaca actctattag gaaagtcgaa ttaatttcta gaaatgtttc agcagctaca
    29461 gcgtactatt ccaaactcaa ccaactatat atttaaagaa agaagaaagg gaagtcaact
    29521 attttctgta aatttcatga aaaatccaaa caactttgat ggtgtattgt ttttttgttt
    29581 ggaaataaat attcgagtta attttggaat gtttactaga ttttgcacta catgcaagag
    29641 aatactatcc ttttctaaga tagagcagga ctaagatact aacttagaaa tccaccttcc
    29701 cactatcctt ctcctataaa aagtggtaaa atggatgaaa gaaagaagga actgaaatga
    29761 caacattatt ttcaaaaatt aaagaagtaa cagaacttgc tgcagtctca ggtcatgaag
    29821 cgcctgtccg tgcttatctt cgtgaaaagt tgacaccgca tgtggatgaa gtggtgacag
    29881 atggcttggg tggtattttt ggtatcaaac attcagaagc tgtggatgca ccgcgcgtct
    29941 tggtcgcttc tcatatggac gaagttggtt ttatggtcag cgaaatcaag ccagatggta
    30001 ccttccgtgt cgtagaaatc ggtggctgga accccatggt agttagcagc caacgtttca
    30061 aactcttgac tcgtgatggt catgaaattc ctgtgatttc aggttctgtt cctccgcatt
    30121 tgactcgtgg aaagggggga ccaaccatgc cagccattgc cgatatcgtt tttgatggtg
    30181 gttttgcgga caaggatgag gcagaaagtt ttggcatccg tcctggtgat accatcgtac
    30241 cagatagttc tgcaattttg acagccaatg aaaaaaatat catctcaaaa gcttgggata
    30301 accgctacgg tgtcctcatg gtaagcgagc tagctgaagc tttatcaggt caaaaactcg
    30361 gcaatgaact ctatctgggt tctaacgtcc aagaagaagt tggtctgcgt ggcgctcata
    30421 cctctacaac caagtttgac ccagaagtct tcctcgcagt tgattgctca ccagcaggtg
    30481 atgtctacgg tggtcaaggc aagattggag atggaacctt gattcgtttc tatgatccag
    30541 gtcacttgct tctcccaggg atgaaggatt ttcttttgac aacggctgaa gaagctggta
    30601 tcaagtacca atactactgt ggtaaaggcg gaacagatgc aggtgcagct catctgaaaa
    30661 atggtggtgt cccatcaaca actatcggtg tctgcgctcg ttatatccat tctcaccaaa
    30721 ccctctatgc aatggatgac ttcctagaag cgcaagcttt cttacaagcc ttggtgaaga
    30781 aattggatcg ttcaacggtt gatttgatta aacattatta aaccataagg gaggtggtag
    30841 ggatggcaga accaaaccta gaaagcctta taaaagatct ttacaatcat gctcgacatg
    30901 atttgagtga agatttagtt gctgctctcc tagagactgc taaaaaactg cctactacaa
    30961 atgagcaatt gcaggcagtt cgtctctcag gcctggtcaa tcgtgaattg ctcctaaatc
    31021 ccaaacatcc agcacctgag ttgctcaact tggctcgctt tatcaaaaga gaagaagcca
    31081 agtacagagg aactgcgact tctgcgctta tgtatgagga actctttaaa atgctttgat
    31141 tccattgtgg atcaaagcat tttttgtaag atctatctcg tgagcgttca aatagtagta
    31201 acaaggaatc atgagaagtc agccgagccc atagtagtga agaaacttct gtaatggaag
    31261 tggagcgaag tgaggaatcg tcaaacggta ggggataggc tgtttgaagg gtgctacgaa
    31321 aaagataggg taaggtgaaa ttatagcgga atgaaataag atatgaacaa attgattgag
    31381 gaaattaaat gaatttatag aaatctttta gcagtcatgg tgtcctattc cagtttcgaa
    31441 atgctataaa tacgaacaaa ttgattggga aattcaaatc gctttctatc aatgttttag
    31501 aagatacagt gctctagtct aacttcaata tactataaaa gaacagatgg aagcaattat
    31561 gatttacttc tatctttaaa ataaatggtc tgataggaaa aatgtgtccc ctcataaaat
    31621 ataggatagt acgaatggaa ggcttttgta gactggagga cgaaggagtt tagaaactct
    31681 cgttaaaagt tagttgagcc ggagtatggt ggtgtggata attatcaaaa attatttcta
    31741 aatcgaaata gttgacagtt agaacgatga gtgttacaat tgttttattc atttcgatat
    31801 cgaaataaat tggaggtgtt atgaaagatg gtcatttgct agcccatcat attcgtttgt
    31861 tgaatgggcg gatttttcaa aagttactga gtcaagatcc tgaggctctt tataggggtg
    31921 aacagggcaa gattttagcg gttttatgga atagtgaaac tggctgcgca actgcgacag
    31981 atatcgcgct tgcgactggg cttgcgaata atacgctaac gactatgata aaaaagctag
    32041 aggaacaaaa gcttgtaatt gttagtccgt gtggaaaaga caagcgtaag aagtatttag
    32101 ttttaacgga gttaggcaag tcccagaaag aagtggggca tcgtgtcagt cagaaattgg
    32161 atactatctt ttacaaagga ttttcagagg aagaaattca ccaatttgaa ggttttcaag
    32221 aaagaatttt ggcgaatttg aaagagaagg gaaatgaggt ttagaatatg tcacaacaag
    32281 tgaaaaatgc tcacaaccta tacatccatg ccattcaaga cggacgagtt gctgaggctc
    32341 aagcccagtc tgtaggggat acctatatcc aacattcgac aggtgttcct gacgggaaag
    32401 aaggatttgc agccttcttt gcagatttct ttgagcgtca tccagagcgt cagattaaga
    32461 ttgtccgcac cattgaggat ggcaatctgg tctttgttca tgttcatcaa tatctgaatg
    32521 gtggagaagc tcaatgggtg acgacggata ctttccgtgc ggatgagaat ggtcgtattg
    32581 ttgagcattg ggatgtcatt gactactatc gaacacctga aaatgatcaa ctagatcaaa
    32641 tttttggaga ttttgaaatc aaggatttgg acaagaaagc agaaaataaa aaactggttc
    32701 gccgtttctt gacagaaatt ttccaaaatg gggagctaga gcagtggagt gattatgtgg
    32761 cagacgattt gattcagcat aatcatgaga ttggacaagg aagtgctgct tataaaaact
    32821 atgtggctga atatagtgtc acttttgact ttgttttcca actcttagga caaggaaact
    32881 atgtggttag ctatggtcag actcagattg atggcgttgc ttatgcccag tacgatatct
    32941 tccgtttaga gaacgggaaa attgtggagc attgggataa taaggaagtc atgcctaagg
    33001 tagaagactt gactaatcga gggaagtttt aaattgagga caaagaatga ttgaatacaa
    33061 aaatgtagca ctgcgctaca cagaaaagga tgtcttgaga gatgtcaact tacagattga
    33121 ggatggggaa tttatggttt tagtagggcc ttctgggtca ggtaagacga ccatgctcaa
    33181 gatgattaac cgtcttttgg aaccaactga tggaaatatt tatatggatg ggaagcgcat
    33241 caaagactat gatgagcgtg aacttcgtct ttctactggt tatgttttac aggctattgc
    33301 tctttttcca aatctaacag ttgcggaaaa tattgctctc attcctgaaa tgaaggggtg
    33361 gagcaaggaa gaaattacga agaaaacaga agagcttttg gctaaggttg gtttaccagt
    33421 agccgagtat gggcatcgct tacctagtga attatctggt ggagaacagc aacgggtcgg
    33481 tattgtccga gctatgattg gtcagcccaa gattttcctc atggatgaac ccttttcggc
    33541 cttggatgct atttcgagaa aacagttgca ggttctgaca aaagaattgc ataaagagtt
    33601 tgggatgaca acgatttttg taacccatga tacggatgaa gccttgaagt tggcggaccg
    33661 tattgctgtc ttgcaggatg gagaaattcg ccaggtagcg aatcccgaga caattttaaa
    33721 agtgcctgca acagactttg tagcagactt gtttggaggt agtgttcatg actaatttaa
    33781 ttgcaacttt tcaggatcgt tttagtgatt ggttgacagc tctatctcaa catttgcagt
    33841 tgtcactttt gaccttgtta ctagctattt tgcttgcgat tcccttggct gtttttcttc
    33901 gctatcatga gaagctggcc gactgggtct tgcagattgc aggtattttc cagaccatcc
    33961 cgtctctggc cttgttgggg ctctttatcc ctttgatggg aattgggacc ttgccggctt
    34021 tgacagctct agtgatttat gcgatttttc cgattttaca aaatactatt actgggctga
    34081 agggaattga tccgagtctt caagaggctg ggattgcctt tgggatgacc agatgggagc
    34141 gtctcaagaa gtttgaaatt ccacttgcca tgcctgttat catgtctgga attcggacgg
    34201 cagcggtctt gattatcggt acggctacct tggcgacctt gattggggca gggggactgg
    34261 gttcctttat cctgttggga attgaccgta ataatgccag tttgattttg attggggccc
    34321 tttcttctgc agtgctagct attgccttta acttcctact aaaagtaatg gaaaaagcga
    34381 aattgcggac gattttctct ggttttgcct tggtggcttt attactgggt ctgtcttata
    34441 gtccagccct tttggctcaa aaagagaagg aaaacttgat tattgctggg aaaataggcc
    34501 cagaaccaga aattttggct aatatgtata aattgctgat tgaagaaaat accagcatga
    34561 ctgcgactgt taaaccgaat tttgggacga caagtttcct ttatgaagct ctgaaaaaag
    34621 gtgatattga catttatcct gaatttaccg gtacggtgac tgaaagtttg cttcaaccat
    34681 cacccaaggt gagtcatgag ccagagcagg tttatcaagt ggcgcgtgat ggcattgcca
    34741 agcaggatca tctagcctat ctcaaaccta tgtcttatca aaatacctat gctgtagctg
    34801 ttccgaaaaa gattgctcaa gaatatggct tgaagaccat ttcggatttg aaaaaagtgg
    34861 aagggcagct gaaggcaggc tttacgcttg agtttaacga ccgtgaagat ggaaataagg
    34921 gcttgcaatc aatgtatggt ctcaatctca atgtggcgac tatgcagcca gctcttcgtt
    34981 atcaggcaat tcagtcaggg gatattcaaa tcacggatgc ctattcgact gatgcggaat
    35041 tggagcgtta tgatttacag gtcttggaag atgacaagca actcttccca ccttatcaag
    35101 gggctccact catgaaagaa gctcttctca agaaacaccc agagttggaa agagttctta
    35161 atacattggc tggtaagatt acagaaagcc agatgagcca gctcaactac caagtcggtg
    35221 ttgaaggcaa gtcagcaaag caagtagcca aggagtttct ccaagaacaa ggtttgttga
    35281 agaaatgatg ggacagagac t
//
LOCUS       DAVBDG010000018        34321 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBDG010000018 DAVBDG010000000
VERSION     DAVBDG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.018"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..800)
                     /locus_tag="VQ526_000813"
     CDS             complement(<1..800)
                     /locus_tag="VQ526_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2554557.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDT"
     gene            complement(800..1135)
                     /locus_tag="VQ526_000814"
     CDS             complement(800..1135)
                     /locus_tag="VQ526_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HET2554558.1"
                     /translation="MQQITEIITNGAISILVILAGVVVRVVKEYLVKKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKIRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGQE"
     gene            complement(1139..1555)
                     /locus_tag="VQ526_000815"
     CDS             complement(1139..1555)
                     /locus_tag="VQ526_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET2554559.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFVFLHSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            complement(1565..1915)
                     /locus_tag="VQ526_000816"
     CDS             complement(1565..1915)
                     /locus_tag="VQ526_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554560.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTITTINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            complement(1918..2121)
                     /locus_tag="VQ526_000817"
     CDS             complement(1918..2121)
                     /locus_tag="VQ526_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554561.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMIASNFLSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(2102..3484)
                     /locus_tag="VQ526_000818"
                     /pseudo
     CDS             complement(2102..3484)
                     /locus_tag="VQ526_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            complement(5480..8701)
                     /locus_tag="VQ526_000819"
     CDS             complement(5480..8701)
                     /locus_tag="VQ526_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET2554562.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKETMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEHNDHLEITAYHISDDVMQRSITQMS
                     VTSQSCGMALARMVQNTKTALGDFSFNSDIQDRRTFNTTETETLYSVLLDGKHSIAGT
                     WEGELVRDNFAMTVKKSRGENRGVVITTHKNLKDYQRTKNSQNVVTRIHARSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKSVEELQKWAQSKFSNEGIDKVSDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDAFKKAIAYEFDALKEEYISLTFDDKAGIGG
                     SRASGGLSSAADAILGVTESAQEIALEKALQNADLDFDHKAGLLRQEISDDIELAKAR
                     AEEVKRELSDTINQRFNSFDNGPLKETKRKAEEALRNAGASTLLAQEAKRIGLDSVAR
                     LEAFKSQTTSAQTALSGDLDALKRTIANDIRPKQAQAEAEIAKQAEALSRTKNELAGA
                     STLLAQEAKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKQTIANDIRPKQAQAEAE
                     IAKQVEALSRTKNELAGVKSAQATYKETTTRRLSELTNLANGKASKSELTQTAEELAS
                     RIASVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDYVRISFDV
                     TFPVALVNDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSEDYQTNNVF
                     IRFGTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQRADSLDA
                     GVRSLTEGLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQ
                     EILNATKDKASKSELTQTAEELASRIASVQASGRNLFLNSLFKQDIPKTGIWTTSTYT
                     ATIDSESKYLGHKALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPGSTTNQDVTISFY
                     AKANKNGIMLRSRLGNIGYKTGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQ
                     EGTIWIWMPKFEISDVDTSYSEAPEDIEGQISTVESTFKQRAN"
     gene            complement(8702..9424)
                     /locus_tag="VQ526_000820"
     CDS             complement(8702..9424)
                     /locus_tag="VQ526_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET2554563.1"
                     /translation="MIKHNELVIDGVRTSSFPFKIIVHDSPSIALGESKTALLEHGGV
                     SGAIVQTNKHRELVKKTYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVSDLEEVQPGLYMTKATFTCHPTKYFKDSDTQRLTRSGTLTVQGSALAFPKITIVG
                     QSASETSFTIAGQVIRLERLTESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSIEIETVWGWA"
     gene            complement(9421..12180)
                     /locus_tag="VQ526_000821"
     CDS             complement(9421..12180)
                     /locus_tag="VQ526_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HET2554564.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKNVNKAATDASQELRQIDKAL
                     KFDTGNVTLLTQKQEVLQKQVSTTKEKLETLRQAQSQVEQQFKNGDIGADQYRAFQRE
                     VEVTQNVLKGYEGKLANVNQALNENGSATQNNKNQLKELQNEQSQLASEMSKVTSSFK
                     LQESALGSNASEAERNALAQKKIGAQSEIVSKQISNLEQQLEITKKEFGENSTQANKM
                     ESELNQAKTAFNHLNDEMKGTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKL
                     VEVGKEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMA
                     SFAKTSGLDTAQALDLTSRATRAAADGAAFYDKSIESVSESLQSFLKGNFANDAALGI
                     SATETTRNAAANKLYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENV
                     MGNLKQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFNSLPDPVKDFVVILGTV
                     VTAVGVIAPIFLTLQALAEFLKISIGEMIIAALPIIGTAIAIAAAVAAIIVIVKYLWE
                     TNEGFRDAVTTVWNAILAVINTVVSEISDFIMSIFGTVVTWWTENQELIRTSAETVWN
                     AIYTVISTILDILGPLLQAGWDNIQLIITTTWEIIKIVVETAINVVLGVIQAVMQIIT
                     GDWSGAWETIKGVFSTVWQAIQSIVQTIFSAIQSYISNILNGISGTVSNIWNSIKDTV
                     SNVLNAISSTVSSVWEGIKSTISSAINGARDAVSSAIEAIKGLFNFNISWPHIPLPHF
                     YVSGSANPLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNNIMVGGEAGNEAVLPLN
                     DKTLGAIGRGIAQTMGGTSPTINITITGNTVREEADISRIADEVAQRIADELQRRRQL
                     RGGFA"
     gene            complement(12458..12877)
                     /locus_tag="VQ526_000822"
     CDS             complement(12458..12877)
                     /locus_tag="VQ526_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554565.1"
                     /translation="MRKIVLVGDQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETDKELTATNIDISMLSDFDMTFFNRLFWTFAKSANPHIKPYEQFFMEME
                     IFPIQEVGPVLMEMLNASMTTKKHQMSQNQLAKKSSQ"
     gene            complement(12889..13467)
                     /locus_tag="VQ526_000823"
     CDS             complement(12889..13467)
                     /locus_tag="VQ526_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET2554566.1"
                     /translation="MTQENKVTFGLENVHIAPIKTLAADGVITYGDVFRFPGAIELIL
                     DTKGETTPIKADNKDYHFMNSNEGYEGKLKIPHIIDEFATKILGEIKDPQTGVMTEKA
                     DASLTEFAMMFQFEGDKNKTRYVMYYCFASRPSLGSKTKNGTSTNERELSFKASPRPL
                     DTVVKRSITSADDKDAYDNWFKKVYEPTAVAA"
     gene            complement(13479..13802)
                     /locus_tag="VQ526_000824"
     CDS             complement(13479..13802)
                     /locus_tag="VQ526_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554567.1"
                     /translation="MKLSDFAAILEQANLPVTYRAFKIGNAPDLPYLVYYESSPVINS
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWANHKLFFEVQEETFIETERLYVKSY
                     TVYLY"
     gene            complement(13799..14146)
                     /locus_tag="VQ526_000825"
     CDS             complement(13799..14146)
                     /locus_tag="VQ526_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HET2554568.1"
                     /translation="MSNDLADLIAKELAAYSDEVTEEVDKIAEQVTDETVDELKETSP
                     KRYGKYRRSWKKKKLANGSFVVFNAVASLTHILENGHLSRNGGRVAGIVHIKPAEEKA
                     IQNFEKRIKEIGK"
     gene            complement(14143..14442)
                     /locus_tag="VQ526_000826"
     CDS             complement(14143..14442)
                     /locus_tag="VQ526_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HET2554569.1"
                     /translation="MWNHEITLISKKVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEVLK"
     gene            complement(14429..14710)
                     /locus_tag="VQ526_000827"
     CDS             complement(14429..14710)
                     /locus_tag="VQ526_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HET2554570.1"
                     /translation="MDQGQLLELLKLKLGISTDLRDKPLKKIISSVITELTDNLGIEL
                     VGERADHEMFIVDYAAYRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            complement(14713..15003)
                     /locus_tag="VQ526_000828"
     CDS             complement(14713..15003)
                     /locus_tag="VQ526_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554571.1"
                     /translation="MSYKVIRPFKDLADPENHDYAVGDIFPREGYEPTDSFTNGLLTG
                     ANTAGSIFLEVLGDDEPKKPAPETKEVKEEPAVEQEETVEETAEESAEEVEE"
     gene            complement(15015..16187)
                     /locus_tag="VQ526_000829"
     CDS             complement(15015..16187)
                     /locus_tag="VQ526_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HET2554572.1"
                     /translation="MNKLLILGARMRNKADKVVELEESIKELNKRSELEAKKLEQAGT
                     DEEVSAVEKNLEDIQKESDEKEAEKEQLEKEIEDLKNQVEELNRKAPTYPSQEKRGGQ
                     KLEQRDAIAKYIRTGQTRDIVGLKTTDSGSAALIPTEVLKPHFVNKTRNPLLDLVERV
                     KVNSGSGKYPVIKKTDGVMVSIDELKSNPELGKPAISEIDYSIKTYRGYVPVSQEMID
                     DADYDIMSIVEDEVFNQGDNTELSLVTAVLKTATQADAAGFDGIKDIYNKKLKSIYKA
                     SIVVTKSMFAALDKVKDKDGRYMLQTDVASPTGYSFGGKTIYKVDDTVFGNEGDMKFF
                     IGDVTEFVKEFDRSQVSVKWVNNDIYGQLLGLFIRLDIKKADEEAGFFGTYTDVVA"
     gene            complement(16184..16759)
                     /locus_tag="VQ526_000830"
     CDS             complement(16184..16759)
                     /locus_tag="VQ526_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HET2554573.1"
                     /translation="MQKRNSYRATQFQTREEESGDLILSGYFIKFDEETELWPGYCEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFNL"
     gene            complement(16743..17945)
                     /locus_tag="VQ526_000831"
     CDS             complement(16743..17945)
                     /locus_tag="VQ526_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HET2554574.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCKHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDEVVHFV
                     INPDPDIPYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            complement(17963..18181)
                     /locus_tag="VQ526_000832"
     CDS             complement(17963..18181)
                     /locus_tag="VQ526_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554575.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNGEVVTIRQRHMKNLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            complement(18189..19919)
                     /locus_tag="VQ526_000833"
     CDS             complement(18189..19919)
                     /locus_tag="VQ526_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HET2554576.1"
                     /translation="MGNLDKAKKYAQHVLTHREEHCEENILAAERFFRDLENPTFEMD
                     EDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVNYLDENILKMANPSWGVTVSLKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYH
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     KMREKGFKIAAVGMDRKFGREFLTKMKQARFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFACIQALANLG
                     KGGDVMRFFD"
     gene            complement(19912..20304)
                     /locus_tag="VQ526_000834"
     CDS             complement(19912..20304)
                     /locus_tag="VQ526_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HET2554577.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYADMGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            complement(20442..20762)
                     /locus_tag="VQ526_000835"
     CDS             complement(20442..20762)
                     /locus_tag="VQ526_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET2554578.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLNFF
                     KNEK"
     gene            complement(20882..21073)
                     /locus_tag="VQ526_000836"
     CDS             complement(20882..21073)
                     /locus_tag="VQ526_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554579.1"
                     /translation="MVKEYRDDFLGEKAFEKLNKDIDANPEVGFEIVGYTQTAFVNGM
                     HIPLTAILVKWNNFFKESE"
     gene            complement(21237..21779)
                     /locus_tag="VQ526_000837"
     CDS             complement(21237..21779)
                     /locus_tag="VQ526_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2554580.1"
                     /translation="MEFVSPIKDNDNIQAMKDYLREWNEMYYMLFITGLNTGLRVGDI
                     LTLKVKDVQGWHIKLRERKTGKQITRRMTKELKKEMRRYVEGKPFHHFLFKSRQGQNK
                     AITRERAYQIIHEAAEELGIDNVGTHTMRKTFGYKYYNKTKDVGTLQKMFNHSSPAIT
                     LRYIGIEQAELDDALRNFVI"
     gene            complement(21968..22369)
                     /locus_tag="VQ526_000838"
     CDS             complement(21968..22369)
                     /locus_tag="VQ526_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2554581.1"
                     /translation="MKKLSDEELKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSGP
                     TVGISKPTETIAIRIADDPTLKFLEGFKGIINKLLSNLVEEDKEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAIMKGEL"
     gene            complement(22581..22739)
                     /locus_tag="VQ526_000839"
     CDS             complement(22581..22739)
                     /locus_tag="VQ526_000839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554582.1"
                     /translation="MNTHCMKATPLLPWGRLRKSAKKQELLNGASISEFAIEDICTFF
                     GIEIQERK"
     gene            complement(22736..22972)
                     /locus_tag="VQ526_000840"
     CDS             complement(22736..22972)
                     /locus_tag="VQ526_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HET2554583.1"
                     /translation="MNPEIIDNINKPSHYQGANGLEAIDVVHNFVGSLSGASAFFWGN
                     AIKYMLRFQKKNGLEDLKKARKNLDWLIEEMEHE"
     gene            complement(22972..23361)
                     /locus_tag="VQ526_000841"
     CDS             complement(22972..23361)
                     /locus_tag="VQ526_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554584.1"
                     /translation="MIPKFRVWHHELGRLMSVKCMFFQDSEIEEFELNDALMNDYITA
                     YPDEIELMQSTGLKDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTFIGGMPAVYLKG
                     LGDGYAWTEYEEIIGNIYENPELLEDK"
     gene            complement(23358..23872)
                     /locus_tag="VQ526_000842"
                     /pseudo
     CDS             complement(23358..23872)
                     /locus_tag="VQ526_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502465.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(23872..24297)
                     /locus_tag="VQ526_000843"
     CDS             complement(23872..24297)
                     /locus_tag="VQ526_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HET2554585.1"
                     /translation="MKLNELIKKYKKLEGVWNAEGAELVRQIFLQDLEQLDKPQPVKV
                     PQCVAEYIEFKKKNNFHVYGLAWLNGYEVEKEKRYFVKIKGNIKENMLVYGELLKRYF
                     FTKSFSLDDVIYSHTRKELEAANFGWVFDCPGIEIEEVE"
     gene            complement(24287..24616)
                     /locus_tag="VQ526_000844"
     CDS             complement(24287..24616)
                     /locus_tag="VQ526_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554586.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPSMSQLVIAVYLIY
                     RGSEDET"
     gene            complement(24784..24951)
                     /locus_tag="VQ526_000845"
     CDS             complement(24784..24951)
                     /locus_tag="VQ526_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554587.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            complement(25078..25305)
                     /locus_tag="VQ526_000846"
     CDS             complement(25078..25305)
                     /locus_tag="VQ526_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554588.1"
                     /translation="MLELYFVYNGHCKFFLGRFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGEKND"
     gene            complement(25305..25499)
                     /locus_tag="VQ526_000847"
     CDS             complement(25305..25499)
                     /locus_tag="VQ526_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554589.1"
                     /translation="MFILKHGTREDKPFLRSAIISVTGLDISCSEEKKAMRFISRAAA
                     LQVSKALRGSFGNFYPVEVE"
     gene            complement(25514..26284)
                     /locus_tag="VQ526_000848"
     CDS             complement(25514..26284)
                     /locus_tag="VQ526_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2554590.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQERVNELANAENERERKYYLEKFSLYDEILKNATLDNFETPTEKEAEK
                     LAFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLIS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            complement(26424..27263)
                     /locus_tag="VQ526_000849"
     CDS             complement(26424..27263)
                     /locus_tag="VQ526_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HET2554591.1"
                     /translation="MSEIKWIKIKTDIFDDEKMCLIDASPDRDAIIVIWIKLITLAGK
                     LNTKGVLTISKNIVYTDEMLAQTFHRPLNTVRMALEVFEKFGMVEKIDGVIMLPNWEK
                     HQNIDGMEKIKEQNRNRAARHRQKQKLLAQNNESNVTDNVMDNVMDNVTVTHGNALDK
                     ELDKDIEINNNKVMISSSLSENLKNSGIHLTDKSHQQLLDYVGLDGMSFDMLNRAVEK
                     TSGSHKPSFNYLIAILESWKKKGFTSIEQVDEDDRKYKEGKKSRQQNDKTSEQEVRDE
                     WGF"
     gene            complement(27256..27552)
                     /locus_tag="VQ526_000850"
     CDS             complement(27256..27552)
                     /locus_tag="VQ526_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554592.1"
                     /translation="MQELIESLDNLIMIVKELEGRESTSRHFITIWENDYKNLLRVKE
                     YLTDYEKLAKDYRYMTLKNKLLKIEKMELEGRYIYEDMRMKYRANRRKWGARYV"
     gene            complement(27568..27888)
                     /locus_tag="VQ526_000851"
     CDS             complement(27568..27888)
                     /locus_tag="VQ526_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554593.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIQVRNHAYERGREDKWQEIRKNIRREFKGFTYDNEPPVGLRPEPLALPEPKQSA
                     IRFL"
     gene            complement(27974..28231)
                     /locus_tag="VQ526_000852"
     CDS             complement(27974..28231)
                     /locus_tag="VQ526_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET2554594.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCD
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            complement(28244..28957)
                     /locus_tag="VQ526_000853"
     CDS             complement(28244..28957)
                     /locus_tag="VQ526_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HET2554595.1"
                     /translation="MNEIFNFHGQEVRTLIIDDEPWFVGKDVADILGYSKARNAIALH
                     VDEDDALKQGLTDNLGRVQETIIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRK
                     QGGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAKSLLKKRKA
                     RVVVCLGGIDSPAYADKTFAQSVFRQAEIDFKDHFNINRYDLLPKKFADAALAYWMTW
                     EPSTNTKMKIMKLNSFDEG"
     gene            29011..29436
                     /locus_tag="VQ526_000854"
     CDS             29011..29436
                     /locus_tag="VQ526_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554596.1"
                     /translation="MEYQALIQPVISIILAIISGLWSYVASKANNKAEIEKQAKEHSH
                     TVEKLEREFHHQIDTLKQQHAFELEKIKQAHELRLQELEKVSQLDTETDKSMKMNDLT
                     YKVFTGEVDFDKALKIANKASNSKQNLNKRFIQKTSKKS"
     gene            complement(29431..29592)
                     /locus_tag="VQ526_000855"
     CDS             complement(29431..29592)
                     /locus_tag="VQ526_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554597.1"
                     /translation="MNELVWFYFVVIINLIIGFATYYASKRDREKRINGFKKIQDDEL
                     ERVRNKFNL"
     gene            complement(29603..29800)
                     /locus_tag="VQ526_000856"
     CDS             complement(29603..29800)
                     /locus_tag="VQ526_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554598.1"
                     /translation="MNNAAQKVTRIDKDAWEIATELANEYGVSICHIISESVRYCAEN
                     AEFKEMDVVVKRLVVGSKVLE"
     gene            complement(29817..30020)
                     /locus_tag="VQ526_000857"
     CDS             complement(29817..30020)
                     /locus_tag="VQ526_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2554599.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQVELAKQVSVASSVISELLKYG
                     KGSDYVKEKVVDILGIENPWENS"
     gene            complement(30013..30387)
                     /locus_tag="VQ526_000858"
     CDS             complement(30013..30387)
                     /locus_tag="VQ526_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554600.1"
                     /translation="MIETLEIKINKKTYFISARADVPVRYKVMFGRNLFDDFSKIKNN
                     KDETLSLCLLFCMTDKSSEEFCKFFIENNSYITSRHHYEAIRLFEKHFIFKPVTYSLE
                     DIVQILTIYLENTKTQIGGKNE"
     gene            complement(30431..30577)
                     /locus_tag="VQ526_000859"
     CDS             complement(30431..30577)
                     /locus_tag="VQ526_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554601.1"
                     /translation="MNEEDLKELLELLATDYGRGYLDGVVGGLSMLLKISKEAEKYRK
                     EKYE"
     gene            30862..31230
                     /locus_tag="VQ526_000860"
     CDS             30862..31230
                     /locus_tag="VQ526_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2554602.1"
                     /translation="MFVAFDKIKELADKQGISINILEEKLGYGTNTLYRLKRSNPSSK
                     VLKEIADYFNVSADYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            31230..31463
                     /locus_tag="VQ526_000861"
     CDS             31230..31463
                     /locus_tag="VQ526_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554603.1"
                     /translation="MTITINFTEKNSYITDYLTKHGIDTTTMDFDDFMVLMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLSREDIA"
     gene            31463..31726
                     /locus_tag="VQ526_000862"
     CDS             31463..31726
                     /locus_tag="VQ526_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2554604.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            31739..32119
                     /locus_tag="VQ526_000863"
                     /pseudo
     CDS             31739..32119
                     /locus_tag="VQ526_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            32157..32969
                     /locus_tag="VQ526_000864"
     CDS             32157..32969
                     /locus_tag="VQ526_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HET2554605.1"
                     /translation="MQKTVEKILFRVAGVTKYKKAVKEACNMIAEDNGIPEYAKYYSN
                     LSTKELREELEEYGLKVFKYQDLDFFNIELVPEVDNRYDPNAIKVLIFDNHVGYVPAT
                     IAKTIRKYFDNKKYNFVIEGEIKGGPYKEWDEYEEKVVTNNDLDVGFEIYLTIVDSSQ
                     KEVIQSESSEIIDDNISNKEVTETEAIETKTTETEHIEQNIVSDSVADIVNEINLSET
                     SPKKKLPANKIIFSALYLFLVFFGVVGIPIAPFLAVPLTAWSLYKLYKLFRK"
     gene            33144..34292
                     /locus_tag="VQ526_000865"
     CDS             33144..34292
                     /locus_tag="VQ526_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2554606.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSILLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
BASE COUNT         9511 a         7827 c         5979 g        11004 t
ORIGIN      
        1 gtgtccttgt acttgaccca gcctgtcttc atggcacctt ctacatcaaa atagtaccac
       61 ttctcagcga ttttcttcca gcctgtggcc atttcgcctg attggtcaaa gtagtaccaa
      121 ttaccgtctg tgtgcttctt ccagcggtct gaaagcatat agcctgagcc atcgaaataa
      181 taccaggtac cgttgatttt ctcaaacttg tcttttggat aagagccgtc tgagtgtacg
      241 taccagtagc cagtaccatt tttctgccag cctgtcgcag cgctcaagcc gttttcgatg
      301 tcttgcttaa actgctcacg gctaatgccc caacttgcaa gatatggata agggtcaacg
      361 tggtctgagt ggttgtttgg ttggttattc gtgcaatact cgtgcgtttt aattccagct
      421 aaactccctg tatcaagcgt tttcggcaaa cctgcttcat ctgctagatt gcgtagcaat
      481 tcgatataga ggcgatagtc agccataaac tcttccttag ttgaatggct ttcaatcagt
      541 tcaaccgctg cataactctc agcattccaa ccgcccccaa catcccaact tccgttgttc
      601 acaggtccta cctgcatgat gcggccgttt ccgacaacat gtgaaaagaa ccctagttca
      661 gggtcctttc tatagtggta atcagcttca ttttgagcgg ttgagttacg gttgcctgtt
      721 gagtgagcat gtacttgtcg ataaggctgc accccaacct ggggcaagcc tgtacgtagt
      781 ctgtttctat cgatatccat tactcttgtc ctttccaagc gtcgttcatc tgcttcacgg
      841 ctgactccac aaaggtgtcc aagtccttgt ccgtcatact gatattatat ttggtcagct
      901 ctgcacgaat tttagcgcga gcctgtgcca gtttttcatc tcccttgtag ccagtttcag
      961 cagctacctg ctccacggca ttaactgcgt tcttggccaa gatttcaaca atcttgatag
     1021 tcttttctcc accttttttg acgaggtatt ccttgactac cctaactacc acccctgcca
     1081 aaatgactag gatgctgatt gctccattag taatgatttc agtaatttgt tgcatttgtt
     1141 attctccttt tttcgtatca tcatcttttt caagtaatcg ctgaaatact tttacaatcg
     1201 gctgaaaaag agtaacattt ccttttaatt tgcggtaatt ttcaatgaga gattgaaaag
     1261 taaatgcgat gtacccgaga tagattgagt gcaagaatac aaaacctgtc ttttcaggca
     1321 acaaaacgga cgccggaatg aggatcatca gtaagagaac ccctgaaatc ttacgaagga
     1381 gcccgttaat gccgattttg ctcttgtact cgatgtcagg attgataatc gccgcaatcg
     1441 tccctgtcac aaaatcaatg atttccattg agacaatcag tgctagagcg tacaagacca
     1501 aaccatcttc agtctgtacg acacttctta gaaaattgaa aaattcaatt tgcatacaca
     1561 cctcctattc tttaggtttt accgttggat ccgtccagtc aggattgccc tctgcatcaa
     1621 atttcatgat atagaattca tgattcaaca gaacggctac gttgattgtt gtgattgtac
     1681 ccccccactg gttgaacgcc caaacagttt caacatcctt gaattggcga cggccattta
     1741 cgatcacagg acgtttttga acatcacgat acatatagaa gtcatcgctt acattcttgc
     1801 aacgaatgaa ctctccattt tctttcatgt agcgcaaagc gctcgcaaga tcaaatggtt
     1861 ctgtgatttt tgtaaggtct agtaagttat ctgtgttttg aattgtttct gccatgtcta
     1921 ttctcctttg tctgctggtt tagtttgttc atcaagcaga gcttccagct catccactcg
     1981 tgcttgaagt ctttgattct cttccctttg ctcattcaac tgaatactca agaaattact
     2041 tgcaatcatc gaatctgttg aagttgttga catttcacga attgtcattt gtaaggcttg
     2101 gttaagctgt tctgtgttca ttttctaagt tctccaatct gtgtgttcgt tttctatttt
     2161 caagagcaaa ctcctgaatt gctttaagtg cgatattggt caacatgttg ttattcatgc
     2221 tattgttttc tccatttttt ctattttttg atttaattct tgaatagcct tgattaagta
     2281 aggaactaaa gcggtatagt cgatatgtag atagccatct ggattctcag gatctcgtga
     2341 gacaattctt ggaacgatgg tttcagcctc ttgagctatt agaccaatct cctcgtgttt
     2401 cttattttcg atgaaatcaa atgcaaccat tcttaatctg ttgattttgt ccaaggcttt
     2461 cacagctgta tctgtgatgt tctcttttaa gcgtctgtct gatttttgtt ccatccaata
     2521 cttcacgcta ccgctaccga cctgattcca ccaaacaacc gcattccttc cgcctttggg
     2581 attccaacca tcaccaagca catctttact tccaagttcg ataccatttg aaaacacagg
     2641 agaacgagaa aaagtagtat tcccatagaa gtttgctctc gatgaattcg aaaaatccac
     2701 ttgatcataa aaaccgactt cattcctaca gtacattttc ccatcagtat tgacgttcca
     2761 tgctttaggt ccggcatagt tccaattatt tccccagttc gcccagaagg ctgtccggca
     2821 ccccatgccc ggcaccattc cccataccaa cagagaactg attgacacct gaaatccagc
     2881 gaccgccacc ctgatcaaat tgaccaagtg taaatccacc gattcggcct tgataggctt
     2941 ctaggaaggt tgaactagaa atgacggact caaccttagt agagaagata cgtttagatg
     3001 tcagttggtc aataaaagcg tcatttgcaa tcatttttct aataaacgca gcatcaaatc
     3061 tcactttatc agccgtgacc gcttcagcgt ctaatatcgt agtcgtgacc gaaccagctt
     3121 caaaattggc cgttttgagc ttatcaacca tggcagactt gatgactgct ctgtcaatca
     3181 gggtctctcc agtgatgtgg gtcaatttcc caacaaagcg gttatgtcca ttggcgccaa
     3241 gattgattcc agagatgata tctccagccg agttgatgtt ttcaactacc catgagccag
     3301 ctagttgagt catttttgtt tgcgtggctt caagcttctt attcgcatct gcgactgcat
     3361 cttctggatg tggttgccat gttctaggtt tataaccttt gtacaagtca acttctgtaa
     3421 tatacaaatc agctgttcct gatgatgagc cattgttatc aaaacgaatg taagcattat
     3481 ccatttctcc ggaattaaaa gttactgaga catcttcgca tctagaggta gatagtttct
     3541 tgctgctaac aactttctta acgattgtga atccatcgct ctcgcctgct cttcgtccca
     3601 aaatataaac atcatagctt gcgagagcac tgttgttaaa tcctctaaaa ttcagtacat
     3661 agtcagtatt tcgttcaaga ttaaaacggt gactatacaa aaagttttcg tttttagttg
     3721 cattacttaa acgcataagg tctttctgcc cgttgtgata aaagctatgc ttaaccaatc
     3781 ttcctaaatt ttgagttgag ccccattcat tcgtaccatt tttaaaatca ctattcttaa
     3841 tgaggttagg gccgcttaca ctatatttcc caacctcaac ctgaaacagt tgattggtca
     3901 gagccatgcg agcaacctta tccgcaattc cattttcagt attgcccaaa atccgctcgt
     3961 aaagtttact ggtttcctta acacgctgga agtcagtagt ctctactttt cgcgctagtt
     4021 gattggtcac attcgcaaat tgactatcag cattcgcttt gtttgcagaa acctgatcag
     4081 atattctacc catttgtcgt tcagcattat ccttgtttgt agcgacctga gtctttaaat
     4141 ttgaaatctg attatctgtg ccttgtttat tactgtttat ccgatttgaa atatttgaaa
     4201 tctgagtagt ggttccttgc tcactgcttg taagtctatt tgatagacca ctgatttgac
     4261 cgcccacatc ttgcttataa gtagttatct gacttgaaat atccgtgaac ttaccatcta
     4321 cagattgacg atagctagcg atttgactag cgatgtcttt attcgcacta gttttaacag
     4381 cttcaatcct ctgattgata cccttaacat cttcttgata agtagcctta ccaacgaaat
     4441 cacgattgac cagctcacgg actgctgtcg cttgtctcgt gctctcctca cgagtatagc
     4501 gctgtagggc ttcctgtcgc tgaccgtctt tatttacata ttcctgaata gctgataagt
     4561 cggttcgcaa gccctgagct gtccgctcaa aggttgcctt agcctcagtg atgaggcctt
     4621 cggtgtcttc cagagctggg ctccaatcgc tttttaagcg acctttcgag actttaggct
     4681 ttttaaggta caaaattgag ttgctagcaa aattatagcc gtaaatgtaa aattggactt
     4741 tttttgtcag gtcaatgtca tcattgacct ttaagtcaat tcctagccta gtccaacggc
     4801 ctttttcagt aacttcaatt tttgttcgac ctgcgtaaaa attgagtttg ccagtcgagt
     4861 tgtgatagta aaatccaaat ttgatatgag tgcctaaatc tgtcgcatac aaatcagctg
     4921 aaaagtaata ctctccagct gagatgactc cgattttaaa atcagttgac aatgctttcc
     4981 agtgtcggga gccgtcgctc tcgttaaagg caaagacagt atcactaaac gtttccgtgt
     5041 caaaataccc atgctggata ttttgagcat ttggaatacc agcattgtac ggattgtacc
     5101 agtaaggcgc aatcatgttt tgataggtct tgccgttagc tgtataacgc tgtccgttat
     5161 ccctcagatt tttaaaatct gagtttacaa acagattctc gccaaccgac agacttgtca
     5221 gctcctctct caacttccca gcctccgctg ttaccaaggt cttgtcagcc ttgtccttgg
     5281 ttgcattcag gatttcctga cggattccgc ttgctcgcac ctcaaattca gcctgactca
     5341 acttctgatt tagcttgttc tgcgtgtctg tctcaagact cttcaccgac tgcctaatat
     5401 tttcagcagt cacgttgagt gagctgatat ccactttggt tctaaggcct tcagtcagac
     5461 ggcttacacc agcgtcgagt tagttggctc gttgtttgaa ggtcgattca actgttgaaa
     5521 tctgaccttc tatatcttca ggagcttctg aataagaagt atctacatcg cttatttcaa
     5581 acttcggcat ccaaatccaa atggttcctt cctggttgaa attgaacaac cattcatttg
     5641 tggtctgctt ggattcgttt gtccaacctt ttggaatatg gacaacatat cgcttaattt
     5701 ctgtcgacaa tgtcacattt ccagttttat atccgatatt ccctaatcga gatcttagca
     5761 ttattccatt tttatttgcc ttagcataaa aactaatggt tacatcttga ttagtcgtac
     5821 ttccgggaat tactttcccg aattgaccca gagctggata agtaaccttg ggattacctc
     5881 catcacggcc agatggattc aaacctataa ttttaagagc cttgtgtcca agatacttac
     5941 tttcgctatc gatagtagcc gtatatgtac tcgttgtcca aattcctgtt tttggaatat
     6001 cctgcttgaa tagtgagttc aagaatagat ttcgaccgga tgcctgcaca ctcgcaatcc
     6061 gactagccag ctcctcagct gtctgcgtga gttctgactt gctggcttta tccttggttg
     6121 cgttcaggat ttcctgacgg atagagccgg ctcgcacctc aaattcagcc tgactcaact
     6181 tctgatttag cttgttctgc gtatctgtct caaggctctt cacagattgc cggatatttt
     6241 cagcagtcac attgagtgag ctgatatccg ctttggttct aaggccttca gtcaagcttc
     6301 ttacaccagc gtcgagtgaa tcagcacgct gtttaaagtt ggattcgact actgagacac
     6361 ggtcttcttg gtcttcatac gctggttgat aggctggaaa ataattacca accgataaca
     6421 tagcgttctc aatgacgacc tgcagaccag caggaaatcc ataattagta ccaaaacgaa
     6481 tgaacacatt attagtctga tagtcctcag aagaactaga caagtcaatc gtaaactcaa
     6541 aatgttggcg ttcgacagtt ccacctttaa aaattaagtt tctgtaggca taccatggat
     6601 gagcactaaa atgcaccata gcaggcatgt catttactag ggcgacaggg aaagtcacat
     6661 caaaagatat gcgaacataa tcacgcttga acctgtcact gttcttccag aaatcaggaa
     6721 ctatgaatgt tcgatagtcg tataccgctt gacctcctgt tgtgaacgtt cttgaacgtg
     6781 aattcctgaa gtaattccgt gaactacctg cctgcacact cgcaatccga ctagccagct
     6841 cctcagctgt ttgcgtaagt tctgacttgc tggctttacc attggccaag ttggtcagtt
     6901 ctgacagtct acgagtcgtc gtctccttat acgtcgcttg cgctgacttc acgccagcca
     6961 gttcattctt agtccggcta agtgcttcaa cttgcttggc aatctcagct tcagcctgtg
     7021 cttgcttcgg tcgaatatcg tttgcgatag tttgttttag aacatccaag tcacctgaca
     7081 aagccgtctg agcgctcgta gtctgcgact taaacgcttc aagtctagca acagaatcca
     7141 gctcaatccg cttagcttcc tgtgcaagta gggtacttgc gccagccagt tcatttttag
     7201 tccggctaag tgcttcagct tgcttggcaa tctcagcttc agcctgtgct tgcttcggtc
     7261 gaatatcatt cgcgatagtc cgtttcagag cgtccaagtc acccgacaga gccgtttgtg
     7321 cgctcgtagt ctgcgactta aacgcttcaa gtctagcgac agaatccagc ccaatccgct
     7381 tagcttcctg tgcaagcagg gtacttgcgc cagcatttcg caaagcttcc tcagccttgc
     7441 gcttagtttc tttcaatggc ccgttgtcaa agctattaaa tcgctgattg atagtgtcag
     7501 acagttctct cttgacttct tcagctctgg ctttggccag ttcaatatcg tcagaaattt
     7561 cctgtctaag caatccagcc ttatgatcaa agtctaagtc agcattttga agagcctttt
     7621 caagggcgat ttcttgtgca gattctgtca ctccaagaat tgcatcggct gcgctagata
     7681 ggccaccaga agctctagaa ccaccaatac ctgccttatc atcgaaagtc agagagatgt
     7741 attcttcttt taaggcatcg aactcataag caatagcttt cttgaatgca tcgacattat
     7801 gtttccagct cttgagattg accgtgtcgc ccatatggac cacttgccca tcaagttcat
     7861 aagcttcaat tttgatagca tcagagacct tgtcaattcc ctcatttgag aacttagact
     7921 gtgcccactt ctgcaactct tcaacggatt ttgcgttgtt gttctcatac tctttttcgt
     7981 ttatataagg ataagaatta ataagaggac tatcaacagt cactctgata gttgtttcct
     8041 tttcagcacc ttcaggctta aatgtggatc gagcatgaat ccttgtaaca acattttgac
     8101 tgtttttggt tcgttggtag tccttcagat tcttatgtgt tgtaataaca acaccacgat
     8161 tctcaccacg actcttcttt acagtcatcg caaagttatc acgcaccagc tcgccttccc
     8221 acgttccggc gatactatgc ttgccgtcca gcaatacaga gtacagggtt tctgtctcag
     8281 tcgtgttgaa tgtcctacgg tcctggatat cactgttgaa tgagaagtct ccaagagccg
     8341 ttttggtgtt ttgtaccatg cgagcaagag ccatgccaca actctgacta gtcacactca
     8401 tttgtgtgat agatcgttgc atcacatcat ctgaaatgtg ataggctgtg atttccagat
     8461 ggtcattgtg ctcaacaggt ttcttgatac gaaatagctg cgctcctaag acaggagtcg
     8521 gagcctttat cagcatatct tcttggatga gctggtaaat accagaatca gaaataggat
     8581 atttcacagt caaggtaaaa tcgccattca tagtttcttt cacaatcgcc gaagtcgctt
     8641 catgaagtgg ctccccgttc catcgaacgg ttcttacatc tttattaagt agataaagca
     8701 attatgccca cccccaaacc gtttcgattt caatcgattg aatacctggg cctaaaacaa
     8761 ccccaatatt cttcactttc gctggatcaa ctgtgataaa atcccctgac catttgactg
     8821 gcttccctgt tgttgtttta aaacttggat tgtcaggatt attaaccatc acaagcgact
     8881 cagtgagtcg ttcaagacga atgacctgac cagcaattgt aaatgaagtt tcagaagcgc
     8941 tctgaccaac gattgtgatt ttaggaaagg caagagcaga accttgaacg gtcaaagtcc
     9001 cacttcttgt caatctctgt gtatcggagt ctttaaagta ttttgtagga tggcaagtga
     9061 aggttgcttt ggtcatgtaa agaccaggtt gcacttcttc aaggtcgctc acattgacct
     9121 tatagcacca aagacgagtt gttttgactc gctcactctc tagccagaac ttttcacgga
     9181 taaacagact cataaattgg ttcatctgtt cttcagtagg tttgaccaag taaatcgtat
     9241 aagttttctt gaccagttcc ctatgcttgt ttgtctgaac gattgcccca ctgacaccac
     9301 catgctccaa gagagctgtc ttgctctctc ctagagcgat tgaaggagag tcatggacaa
     9361 tgatcttaaa tggaaaagac gatgtcctca caccgtcaat cacaagctca ttatgcttta
     9421 tcatgcaaac cctcctctca attgtctcct acgttgtaat tcgtcagcaa tacgctgagc
     9481 cacctcatca gcaatccgac tgatgtcagc ttcttctctg acggtgttac cagtaatagt
     9541 aatgttgatg gttggtgaag ttccacccat agtttgagcg atacctcgac cgatggctcc
     9601 aagcgttttg tcgttaagtg gcaatactgc ttcattccca gcttcaccac caaccattat
     9661 gttattacca ttcattccaa agatagttgg tttcgtcatg ataccgcctt tggcatacca
     9721 ttcgattcca atacttggaa caccttgact caaccaatct aatggattgg ccgaaccact
     9781 cacgtaaaag tgaggtagtg ggatatgtgg ccagctgatg ttgaagttaa acaatccttt
     9841 gatggcttca atagctgaag aaacagcatc ccttgcacca ttgatagcac ttgaaatggt
     9901 actcttgata ccttcccaaa cacttgatac agtactagat atagcattta acacatttga
     9961 gacagtgtcc ttgatgctgt tccagatatt tgatacagtt cctgaaatgc cgttgagaat
    10021 atttgaaata taactctgga tagctgagaa aatagtctga acaatgcttt gaatagcttg
    10081 ccatacagta gaaaacactc ccttgatagt ttcccaagct cctgaccaat caccagtaat
    10141 gatctgcata actgcttgga taacaccaag gacaacattg attgcagtct caacaacgat
    10201 cttgatgatt tcccaagttg ttgtaatgat cagttgaatg ttatcccagc cagcttggag
    10261 caaggggcca agtatatcca gtattgtact gatgaccgta taaatggcat tccagacagt
    10321 ctcagcactt gtcctgataa gttcctggtt ctccgtccac caagtaacaa ccgtcccgaa
    10381 tatactcatg atgaaatcag agatttctga aacaactgta ttgataacgg ctagtatagc
    10441 attccaaacg gtcgtgaccg catctcgaaa accttcgtta gtttcccaga gatattttac
    10501 aataacaata attgcagcaa ctgcagcagc aattgcaata gctgttccaa taattggcaa
    10561 tgcggcaatt atcatttctc caatagatat ttttaaaaac tcagcaaggg cttgcaaggt
    10621 taagaatatg ggggctatga cccctacagc agtcacaact gttcctaaaa taacaacaaa
    10681 atcttttact ggatcaggca aggaattgaa cagctcggcc acacctttca caatcgttgc
    10741 caaggtttga aaaacaggaa tcatcatttc tagaagaggt tgaccaatag cagataatgc
    10801 attggtccca gcttgtttca gattccccat cacgttttct aggccgtctg attctcttgc
    10861 agcctgtcca agagctcctg agagtttatt tccgtcttcg accatctgaa gcaaggtcaa
    10921 ttgcttctgc gcttcgctca agtccttgaa tgactttccg tacaatttat ttgcagctgc
    10981 attcctggtc gtttctgtcg cagagattcc aagagcggca tcgttagcaa agtttccttt
    11041 caggaaagat tgtaggcttt ctgatacgct ctcgatggac ttgtcgtaaa aagctgcgcc
    11101 atccgccgct gccctagttg cacgggaagt caggtccaat gcttgcgcag tgtctagtcc
    11161 tgaagtttta gcaaatgaag ccatctgagt aaatgaccct tgtaaacgct ctgggacaat
    11221 atccatttcc tgaccaatag cattcaacgc ttctcttgct tgggtttcca tatctccgaa
    11281 aacggtagta aattgagcat tactagcttg catttgagca gctgcttcta acgcttcttt
    11341 tcctacttcc acaagctttt ctgaaatagc actcaacttc tcactaaact gttgaagtag
    11401 ttctgctctt aaatttcttg agatttcact taaactttct tgagtgctat cagcagcaga
    11461 ctttgttccc ttcatctcat cattgagatg attaaaagca gtcttagcct gatttagctc
    11521 agattccatc ttgttggctt gtgtggagtt ctcaccaaat tcttttttag tgatttccaa
    11581 ttgctgttct agatttgaaa tctgtttact tacaatctca gactgggcac caatcttttt
    11641 ctgggcaaga gcatttctct cggcttcact agcatttgaa cccaaagcgc tttcttgcag
    11701 tttgaatgaa cttgtcacct tactcatctc tgaagcaagt tgactctgct cattttgcaa
    11761 ctcttttaat tggttcttgt tgttctgagt agcactccca ttctcattaa gcgcctgatt
    11821 cacatttgca agcttaccct catatccttt taggacgttt tgagtaactt cgacttcgcg
    11881 ttgaaaggca cggtactgat cagcaccgat atcaccattt ttgaactgct gttccacctg
    11941 agactgagct tgtctcaagg tttccagttt ctccttggtc gtcgaaactt gcttttgtaa
    12001 gacctcttgt ttctgagtca ggagcgttac gttccctgta tcaaatttca aggccttgtc
    12061 aatctgtctc aactcctgac ttgcatcagt agcggcctta ttgacatttt tcagcgcctt
    12121 ctgcaagggt tgcgtgtcgc catcgatttc aattttgata cctttgatat ttcctgccat
    12181 atttcctcct ttctcaaaaa aatagaaaag cgctgagaga acttctacca ctgataatgc
    12241 agccagacca aggaacttgg tctcagaatc gctctctcag cactcatttt tctttaaaaa
    12301 ctgtcaaaat cagcttgcgt ggctttccgt tcgccaccct tatcctcgct ccgtagattc
    12361 acataatccg tctgataatc cagagccatt ccgattgaaa tgtgctttag atcatcaata
    12421 gaaagaccag tttctttaca gcaagataga taggattcta ctgtgaagat ttcttcgcta
    12481 gctgattctg actcatctgg tgcttttttg tcgtcatgct cgcatttagc atttccatca
    12541 gcacaggtcc aacttcctga atcgggaaga tttccatttc catgaagaat tgttcataag
    12601 gcttgatgtg aggatttgca gatttagcaa aggtccaaaa aagacggttg aaaaaggtca
    12661 tgtcaaaatc tgacaacatc gaaatatcaa tattagtcgc tgtcaattcc ttgtcggttt
    12721 ccaacttgtt caattcattc atgaatgatt gatttttcaa catcgagaac aaatcttgaa
    12781 aataatcttt tccaaattgt tgcttgtaag caataggagt atagccgttg gtccccaact
    12841 catactcctg atcaccaacc aaaacgattt tacgcataga tcttctcctt aagctgcaac
    12901 tgcagtaggt tcatacactt tcttgaacca gttgtcatac gcatccttgt catcagctga
    12961 tgtgatagaa cgcttgacaa ctgtatccaa tggacgcggg ctagctttga aactaagttc
    13021 acgttcgttg gttgatgtcc cgttcttagt ttttgagcca agagatgggc gactggcaaa
    13081 acagtagtac atcacatagc gagtcttgtt tttgtcgcct tcaaactgga acatcattgc
    13141 gaactctgtc aagctcgcat ctgctttttc agtcataaca ccagtttgag ggtccttgat
    13201 ttcaccaaga atttttgtcg caaattcatc aatgatgtgt gggattttaa gtttaccctc
    13261 atatccttcg tttgaattca tgaaatggta atccttgttg tctgctttga taggggttgt
    13321 ttccccttta gtatcaagta tcagctctat tgctccagga aaacgaaaaa catcgccgta
    13381 agtgataact ccatctgctg caagtgtctt gataggtgcg atatgtacat tttctaggcc
    13441 aaaggttact ttattttctt gagtcatgtc attcctcctt agtatagata gactgtgtaa
    13501 gacttgacat agagtctttc agtctcgata aatgtttctt cttgaacttc aaaaaagagc
    13561 ttgtggtttg cccacagctc ttccagacgt tcttccaaat cttcatcctt actctcaaaa
    13621 gccagctcaa ctgtcacgct cttaatctga tgattaaccg tgttgtcagc tgaattgatg
    13681 actggacttg attcataata gaccaggtaa ggtaggtcag gagcgtttcc aattttaaac
    13741 gctcgataag tgacaggcaa gtttgcctgt tccaaaatag cagcaaagtc tgatagcttc
    13801 atttcccaat ctccttgatt cgcttctcaa agttctgaat tgctttttct tcagctggct
    13861 tgatgtggac gataccagcg acacgaccac catttcttga aaggtgcccg ttttcaagta
    13921 tgtgagtaag acttgcaact gcgttgaaca caacaaaaga gccattggcc aacttcttct
    13981 ttttccaact tctacgatac tttccgtacc gtttcggact tgtctctttc aactcatcca
    14041 cagtctcatc agtcacttgc tctgcaatct tatccacttc ttcagtaacc tcatcagagt
    14101 aagctgcaag ctctttcgct atcaaatcag caaggtcatt actcatttca acacctctga
    14161 caaagtcaac tctaaaattt cagaatcaat aggataggtt ttcaagatat gatattgctt
    14221 gccttcgaac ttcgcaagct cttgattctc atactcaaaa tttcgaatct caacgaccaa
    14281 gctcggttta agccctacct gattcgcctg ataaaattca gagcgagtga ccctcttttt
    14341 gcgacataag agagtaactt caacatcttc agagattggt tgtagtaact tatccttacc
    14401 tgttactttc ttggagatca gtgtgatttc atgattccac attcttgacc tctttctttg
    14461 atgctatctg taaattatgc agtcgccact gaaggtgacg tggcatatcc accccaccct
    14521 cataacgata agcggcatag tcaacgataa acatttcatg gtcagcacgc tcaccaacaa
    14581 gctcgatacc gagattatcg gtcaattcag tgatgacact tgaaatgatt ttttttaacg
    14641 gcttgtctct caagtcggtt gaaataccca acttaagctt cagcaactct aaaagctgac
    14701 cttggtccat gtctattcct caacttcctc agcagactct tcagcagttt cctcaactgt
    14761 ttcttcctgc tcaactgcgg gctcttcctt cacttctttt gtttcaggcg ctggtttctt
    14821 aggttcatca tctcccaaaa cctcaaggaa gatagagcca gcagtgttag cgccagtcaa
    14881 aaggccattg gtaaagctat ctgttggctc atatccctca cgaggaaaga tatcgccaac
    14941 agcgtagtca tgattttcag gatcagccaa gtccttgaaa ggacggatta ctttatagct
    15001 catacgctac ctccttaagc tacaacatca gtatatgttc cgaagaatcc agcttcttca
    15061 tctgctttct taatatccaa acggataaaa agcccaagca attgtccgta aatgtcattg
    15121 ttcacccatt taacggatac ttgagaacgg tcaaactctt tgacgaactc agtaacatct
    15181 ccgatgaaga atttcatatc tccttcgttt ccaaacactg tgtcatctac tttgtagatt
    15241 gttttcccac caaatgaata gccagtaggt gaagccacat cagtttgaag catgtagcgc
    15301 ccatctttgt ccttcacttt gtcaagcgca gcaaacattg acttagttac aacgatgctt
    15361 gctttataaa ttgatttaag cttcttgttg tagatatctt taataccatc aaatccagcc
    15421 gcatctgctt gggtagctgt tttgaggaca gctgtaacta atgacaattc agtgttgtca
    15481 ccttgattga acacttcgtc ttcaacaatg gacatgatgt catagtctgc gtcgtcaatc
    15541 atttcttgtg acacagggac atatccacgg taagtcttga ttgaataatc aatctcgctg
    15601 attgctggtt ttccgagttc tggatttgat ttcaattcat ctattgaaac cattacacca
    15661 tccgttttct tgataactgg atatttacca gatccactat taactttcac acgttccaca
    15721 agatccaaaa gtggattacg tgttttatta acaaaatgag gtttcaaaac ttcagtaggg
    15781 attaaagctg cgcttcctga atcagtagtt ttcaagccta caatgtcacg agtttgacca
    15841 gtacgaatgt atttagcgat tgcgtcacgt tgttccaatt tctgtcctcc acgtttttct
    15901 tgacttgggt aagtcggtgc tttacgattt agttcttcaa cttgattttt caaatcttcg
    15961 atttcttttt caagttgttc tttttctgct tccttttcat ccgattcttt ttggatgtct
    16021 tcaaggttct tttcaactgc tgaaacttct tcgtcagttc cagcttgttc caatttctta
    16081 gcttcaagtt cagaacgctt gttcaattct ttgattgatt cttcaagctc tacaactttg
    16141 tctgctttgt tgcgcatacg agcgcccaaa atcaataatt tgttcataga ttaaatttct
    16201 ccttaatttc tttcttgcgc ttgtctagcg cttcacgatt agcacgcttc tgactttcga
    16261 agtctttttg tcgtgcagca atttccgttt gtggataggc tgggaaagta catggactca
    16321 cttcaaagat ttctagctct aagacagtgt ccaaatatga accatcttca cgttcttccg
    16381 tttcgatttt taccgggata aagccaaagc tacaccctat aacatctcca cgcttgacac
    16441 gggcataggc cccaacagct tgaggatcat ccttgttaat gatgatatct ccaaaaagac
    16501 caacatcatc aacacccagt gtcagagttc cgttacctgt tcgaccgaga acaaggctat
    16561 catcatggtt aaataaagct ctgatatcag cgtctgtgac agctttctca acaccagcac
    16621 gcttgataac ttcacagtag cctggccaca attccgtctc ctcgtcaaac ttgataaagt
    16681 agccactcaa aatcaaatca ccagattctt cttctctcgt ttgaaattga gtggcacgat
    16741 agctatttcg cttttgcatt ctcttcctca cctcccttca gtttattttg atctcctagt
    16801 ttctcctgag ggagaaagtt ttcaagaaca atcaactctt ccatctcagg atcaggagcc
    16861 atccctagcc aatccctcca ctcattacga cgcattgcag cactgtttgc catttgttga
    16921 gcaacagcag acaactccgt aatgttgtaa gagaagagtg aacgaggatt tagcttgaag
    16981 taacgattgc tagacaaaag taagtccctg gttagtgttt gagtaatagt ggtagcaata
    17041 ctcatgacag tcgtatttac aaagttgtta tactctgtct tgttgaactc tcctacaccc
    17101 aaaataaaag caggtactcc taatagacct gcaactgttc ttttatccaa ttccacagac
    17161 tcgtttaaag cgatgtccgt tagactaagc ggttttacct gctgaatgtc cagcaatgcc
    17221 tctggaacaa tccatggagc gccaaccctg ctagtactca aatacttctc agcgatacgc
    17281 tcacgccctt gctccgaatc tagttcagca ctagacgagt ctactttaac gataagacta
    17341 ggaatattct tcccgttcat aaagcttttt ttagtcttgg tagccatgtt caaactttga
    17401 accacatctg tcaacgtcac cctaaaacca gtaccaatgt atggaatatc tggatctgga
    17461 ttgatgacga agtggactac ttcatcaggg gaatattctt cacccctaaa tgagattaca
    17521 taggaatcct tatctgtttg gaatgaaacc tctctcatcg gaaatggtct tagattagaa
    17581 atataatccg taacaggttc atattccaca tgtaggacag agttcccatc gccatataaa
    17641 agcaaatcgc gcacaatctt gaaaatccat gacttccttg tcatgtgttt acacggattg
    17701 atatcaatct ttctagcaag cccatcacgg attctgatat cacctttatc tgtattctcc
    17761 atcaagtgga tggtcatatt agagaccaaa tcagcaatct tattaaccgc tgtcaccaca
    17821 tctggatttc tggccaaagg tacatacgag tccatcaggt ttgacaaccc taaatctgaa
    17881 tgactcagca tgttgattga cttacttggc ttgtttcgtt tccaaaactt ttcaaaaata
    17941 cccatgtttc ctcacctcct ttctaacgaa aagtattttg aaaaagtgaa tcaaagtgtt
    18001 tatttcttac gatattctga ctgacatcaa ttacctgttt ttcccatttt actgtttcag
    18061 ccatcaaatt tttcatatgt ctttgacgaa tagttactac ttccccgttt aaaattactt
    18121 taactcgccc tttgttgatc agcaagttaa tttcatgttc tgataatact atttcattca
    18181 taattcacct aatcaaagaa tctcatcaca tcaccaccct tgccaagatt agcaagagcc
    18241 tgtatacaag caaagacgct ggcatcaaac aagtcaatcc ttgcagtccc accgtctcca
    18301 tctaatttct catattgcac agcatcgtcc accttttcaa ttgctctaac attgctcaca
    18361 cagtattcgt aagcatcaga atgaagatag taaaattctt tattcttaac tttaaactca
    18421 atccgtctga atccctctga tttcagataa aaaagctgag gttggtcaat catcttgaac
    18481 cgagcttgtt tcatcttcgt caagaactca cggccaaact tcctatccat tccgacagca
    18541 gcaatcttga accctttctc cctcatcttg atgaaccatt tgacgatatc atcatagagg
    18601 acggtcggag tgttgctcat cgtcaaccaa ccgtcagact gccacccaaa gagtggaatc
    18661 ccgtcatcat tagctttttt ctgagcatta atccgaggga agaaagcatg tgtgatacag
    18721 atatcaacgt ctttctcacc gtcatgatag acaccataga gagcagcagc tgttaagtcg
    18781 tgcaatcttg acaagtccgc accaccatac caacgaatag gcaaacgtgc cagctcttct
    18841 aaactccaat cgtagcaact atccgacgca ataaactcat caggattaaa ataagcgttc
    18901 atagagttag tgaagatatt caaggtctta ttgaaaaact catttcttgt ctgaggatca
    18961 ttcatagcct gctctgcttc ttccttgaga gccttgagcg aaaccgtgac accccatgac
    19021 ggattagcca tcttgaggat attctcgtcc agatagttca ccacgtcccc atcagcagat
    19081 tgattagcct tgcagatgaa gatgaaaaat gaatcatcag tgactaattg cttgagcacc
    19141 ttttgacagt atttcagacg gttagcaagg aacccggtag gaatatcccc agccgttgag
    19201 ataacaaaaa gcatactgtt tctgtatgct gacattgttt ttttcataag accatgtttc
    19261 ttactgttcc tcattgtgtg agcttcgtcc aagatgataa catttccgtt caaagagtcc
    19321 agacggctct catcattcgc taaagcctgg ataaagaaag aaccttcatc accaaaattg
    19381 gcggtgatag agtgttcttg gttattatcc ttgatacgaa tgttcttatc gttccagcgt
    19441 tcaacgttga atcttaaaaa accaaaagct tccatcgctt gcttgactga gttagcaacg
    19501 atatagcatt tggaaccgct atctgtatct aatatctgat aagcaagtgc gattgcagca
    19561 gtaaacgagg tcttcccatt cttccgagca agcatgataa gcgcttcttt gaacctgcgc
    19621 tcattcgtac ccttgtagta aaaaccaaac agattaacta caacgaaatg ttgccacggt
    19681 tgcaaaagca atggcttgtt acggatagat accgcaaaca tatcatcgcc ctgctgatga
    19741 actatcacgt tctcgataaa gtgaataaca aaatccacca tatcctcatc catctcaaag
    19801 gttggatttt ctaaatcacg gaaaaaacgt tcagcagcaa ggatgttttc ttcgcaatgt
    19861 tcttctcggt gagttaagac gtgttgagcg tattttttag ctttatcaag attacccatt
    19921 gccagtcact cgcttcttct tgatttcgtt cttgaacttc agtacctcag taagaactga
    19981 ctcaccctct tgttctacta cctcaccgag cgacttagga ttcatcatca actgattaga
    20041 gtagctgagt atatctttcc tcaaaatttc catcgctgtc aagattggaa ctttgcgctc
    20101 attttcagca ccagccttat tgacgtaggt gtctgttact ggataaccca tatcagcata
    20161 atcttgagca agtttctgat actggtataa catacctgca aaaatgtcaa tgatcatttc
    20221 gaactcttta cgataagttc ccaagtcttt catctgcttg accacttttg acttaatcga
    20281 ctttgctgta attggtttag ccaaaaacta cctcctttcg tcaaaatcgc ttagttttta
    20341 cccccttttt gtttgaaggc ccccgacttg gaaaaagttc ccttcaccag taccctactg
    20401 gccaaaatga tttttcaaaa agaggggggg attaaaaatt ttcatttttc atttttgaaa
    20461 aaatttaaaa attctttttt tctttttttc tgccaataca atccttggtt gattactcta
    20521 tcgttcactc tatcatgaaa cgtattatgt ttcttattcg tcaatggcaa gcaattccat
    20581 tcaacaaatt caagctcagg atattcagac acaggaaaga tatgatgtac catttctgct
    20641 tgaacagaaa ttccgtaacg caaactttct tggcaaagat aatcatgctt acgcattatc
    20701 ctatcacgga acttctccca cttcttagat ttcaaggatg gtctgatagg tttgttatac
    20761 atctcaaacc tcctttctca atgctaaaag ggataggcct ttgacctatc ccctcctcat
    20821 acaagaaatc tatgctacca taataaacct ttttttgtga gacttcaaga tgtcttttgt
    20881 ctcattctga ttctttaaaa aaattattcc attttactag tatggctgtt aacggtatat
    20941 gcattccatt tacaaatgct gtttgagtat atccaacaat ttcaaagcca acttcaggat
    21001 tggcatcaat atctttattt aatttctcga aggccttttc tccaagaaaa tcatcacggt
    21061 attcttttac catttttcac ctcttgtaac tataccaatt ttatccctca ctttcacata
    21121 tcttatattt tgttaaactc actctaaatc tcaaaccctt actaatcata ggttttgaag
    21181 actttcattt tttcagttta tgcttaactc attatgtgaa agtaatatct aaaaaattaa
    21241 atgacaaagt tccgtagtgc gtcatcaagc tctgcttgct ctatccctat gtatctcagg
    21301 gtgattgcag gtgatgagtg attgaacatt ttctgtaatg ttcctacgtc ctttgtcttg
    21361 ttgtaatatt tatagccgaa tgtcttgcgc attgtatgtg tgccaacatt atcaatgcca
    21421 agttcttcag ctgcttcatg tatgatttga taggctcgct cacgagtgat cgctttattc
    21481 tgaccttgcc tactcttgaa taagaaatga tgaaatggtt tgccttcaac atatctcctc
    21541 atttctttct tgagttcttt tgtcatccgt cttgttatct gcttgccagt cttccgttct
    21601 ctcagtttga tgtgccaacc ttgaacatct ttaactttca aggtaagtat atctccgact
    21661 cgtaagccag tattcaggcc tgtgatgaat agcatataat acatctcatt ccattctctg
    21721 agataatctt tcatagcttg aatattatca ttgtctttta tcggtgatac aaattccata
    21781 actgcctcct ttctacaaaa caaaaagcca gctggttgct gactcatgat gttcctctgt
    21841 taaacaactt ttctgtaaaa ataggataac tccaacatgt gatttgtgtt tttgttttca
    21901 gaagttcatg ctatcataat agcccttttt ttgtgagact tcaagatgtc ttttgtctca
    21961 atcttattta caactcacct ttcattatag cgtactgttc taagataatc cttctacgtc
    22021 gatagattgt agctttgctc atgaatttct gttctgctat ttcttcccat ctcagttgag
    22081 ggtatctcca gcgtagattg aaaatttctt tgtcctcctc gactagattg cttaagagtt
    22141 tgttaataat ccctttaaat ccttcaagaa attttaaagt cggatcatct gcgattctga
    22201 ttgcgatagt ttcggtaggt ttgcttattc ctacagttgg cccactttga gcatctgggt
    22261 ttctggtttc taattctagc cttcttaagt ctattgtgcg ttgaatgttt tgaaatttga
    22321 aaagttctct gtctaacgtt ttgagttctt cgtcgctcaa ttttttcaat ttccacctcc
    22381 aagtttttca aaaatgtaaa caagttatca aatatctgag agaaagcctt gcgaatatta
    22441 gaaactgcct gattaatcat tttagttata gcttcaattt cttcagaact taatttccgt
    22501 agttgtttgt ataattcttc ttgcttttct tgtatctgcc gtttagctat cttcttctta
    22561 attcttttgt tcattgactt ttatttcctt tcttggatct caatcccaaa aaacgtacag
    22621 atatcttcga tggcaaactc tgaaatactt gcaccgttca gcaattcctg tttctttgct
    22681 gatttccgca agcgtcccca tggtaataaa ggtgtcgcct tcatacaatg cgtattcact
    22741 catgctccat ctcctcaatc agccagtcaa gattctttct agctttcttc aggtcttcca
    22801 gaccattttt cttttgaaac cgtaacatat acttgattgc gttgccccaa aagaaagcag
    22861 acgctccgga aaggctccca acgaagttat gcacaacatc gatagcctca agaccgtttg
    22921 caccttggta gtggcttggt ttgtttatat tgtcaattat ttctgggttc attatttatc
    22981 ctccaaaagc tctggatttt cgtagatgtt gccgatgatt tcctcatatt ccgtccacgc
    23041 atatccatct ccaagtcctt ttaggtatac agcaggcatt ccacctatga atgtaccacc
    23101 gtattctttt tctaaatata cttcatggag acatcctctt gtacatttaa cgatgtcacc
    23161 gatgaatacc tccttgccgt tcttgtcttt gagtcctgtt gattgcatga gttcgatttc
    23221 atcaggataa gctgtaatgt aatcattcat taaagcatcg tttaactcaa attcttcaat
    23281 ctcgctatcc tgaaaaaaca tacatttgac tgacatcagt ctacctaatt catgatgcca
    23341 cactctaaat ttcggtatca tgccaaatcc tcctctttta caaacgtgcc gtcctttctc
    23401 tctgtgacct tctcaaattt cataaacgtc atccaatgcg tagtacctct ttgttgtcca
    23461 aacaatggtt taaaaggtac agatttaaga acttctttaa cgttgatttg acaatcagac
    23521 catttaaaaa ctagagtacc tccaactttt agaactctca tacattcttc aaaacccttg
    23581 gctaaatctt ccgaccaggt aactttatcc agctggccat attgtgatcg cataattgag
    23641 ttttgacctg cccatttcaa atgtggcgga tcaaatacta ctagattaaa tgtttcacca
    23701 tcgaatggca tatcacgaaa atctccgaca acgtcagggt ttacgtttac ctttttccat
    23761 gtatttcaaa tttttcttgt ctgatgtcca tgaaagttgt atgactctca tttttatcaa
    23821 accaaaacat acgactgcca caacaagcgt ctagtattcg tatatctccc attattccac
    23881 ctcctcaatc tcaatacctg ggcaatcaaa cacccagccg aagtttgcgg cttctagttc
    23941 tttacgagtg tgggaatata taacatcgtc taaactaaag ctttttgtaa agaaatacct
    24001 tttcaaaagt tctccataaa ccaacatatt ttctttaata ttccctttaa tcttaacaaa
    24061 ataccgcttc tctttttcga cctcgtagcc gttaagccaa gcaagaccgt aaacatgaaa
    24121 attgtttttc tttttaaatt ctatatattc cgccacacac tgcggaactt tgactggttg
    24181 cggtttatct agttgttcca agtcttgcag aaaaatttga cgaactagtt ctgctccttc
    24241 agcattccat acaccctcaa gttttttata tttcttaatc aattcattaa gtttcatctt
    24301 cgcttcctct gtaaatcaaa taaactgcaa taactaactg agacatgctt ggcgaatagc
    24361 caacccaatc atcaaactcc ttagattttg gcaaccaacc cttagtagct cccaaatcat
    24421 agtctgtagg cttttcatca gcgaagatgc attccatcgc tcccataaac gtcataccat
    24481 cttctgccat ttcccaaaaa tagtccgccc ggtctttcac cgcttgtggt aaatcttgct
    24541 tgggaggttg cggcttcccg tcttctaccg tccagttgta tacttcatta actttttgct
    24601 ttaactcttc catcatattc caactcctcc gctttccgtc ttaattcttt attctttttc
    24661 ctcaacaaat cgcgctccag cgctctaatc cgtctcttgc gtgcatcgca cggcttcgaa
    24721 tactcgatta tcttctcttc gttttgctcg atcgtgcgtt tcagtccgtc aatctcagcc
    24781 tgtttatcgt acttcatctt ctaaaaatct ttcaatagct tctctgtagg agacttccac
    24841 cagaccgtct aagtcgttca gggcttcaat atagtctgga cgaccttgcc cgtactgctc
    24901 tttcaaaaat tcaacaaaga gatgaatttc ctgataggtt actccaacca tgtttcttac
    24961 ctcccactaa aaatgtaacg ttattgatca tctttctttt cctttcttgc tgcacgttcc
    25021 ccgactaaat agccaagaaa taaccacaga atagccattc caaattcttt aataagttca
    25081 atcatttttt tctcctcctg aaaaagtcgc taaatagtaa caatccttag aaccgtagtc
    25141 aaatcgtgtc gtccgctgac caatgtgctt ctgaaacctt ggatgagtga tagctgagaa
    25201 agcccactga tggtcttcca tctgctcaat gagatcatcg acattgtcaa acctcccaag
    25261 gaaaaactta cagtgcccgt tgtagacgaa ataaagctct aacatcactc cacctcgaca
    25321 gggtagaagt tcccaaagga acctctcaat gccttgctaa cctgtaaggc tgccgcccga
    25381 gaaataaacc gcatggcttt cttctcctca gaacacgaaa tatccaagcc agtcacactg
    25441 ataattgcag accttagaaa cggcttatcc tctcttgtcc catgctttaa aataaacatc
    25501 agccacccct attctaaaaa tattgctttc gcttgtttgt caaatcattg aaaaccatca
    25561 aatggtcttt atctacaccc ttcattagtc tggacatgaa aggtctgcca tatctttttt
    25621 gaatatcggc agaaatcaaa tttgtggtaa tgattgtgtt tgaacgctta ttcaagatat
    25681 tgtagagaat agtaaatgac cattcgctat ctttttccat tcctaaatca tccaaaacca
    25741 agaacttagc actagcaatt ttattgacca gaaactcttc ctgactaaaa tcagctttaa
    25801 tcttcatcag caagtcagtt acgttaataa aaatagcaat ctctttcgtg tactcagata
    25861 gagccttaac catagcaaaa gccaaatgac tcttaccagt tccagcttct ccctgaagta
    25921 caatgttgtt cctagcaccc tcagaccact cacgacaaat cctctttgca aaagctagct
    25981 tttccgcttc tttttcggtt ggtgtttcaa aattgtccaa agtagcattt ttcaaaattt
    26041 catcataaag agagaacttt tcaagatagt atttcctctc tcgctcattc tcagcattag
    26101 ccagttcatt cactctttcc tgattttctt catgaatccg ctcagattca cacatgcgac
    26161 atacaacact ctcggtcctc aatatcttta tcaaagggat gttatgcttt tcgcagaact
    26221 cttcttgttg ttctgtattc ctacgataag ataaggcgat ttcctcaaac acattgtcta
    26281 ccatactagc cgacctccgc attcatgcca gctagccatt tcagacaagc aggcaaccac
    26341 tggatgaatt ggttggtctg ctaaaagagt tttcttctcg tagcttaacg gataatagtc
    26401 aatctcgaac tgctcaatta gttctaaaat ccccattcgt cccttacctc ctgttccgat
    26461 gttttatcgt tttgttgtct tgacttctta ccctctttat atttacggtc gtcctcatcc
    26521 acctgctcaa tcgatgtgaa gcctttcttt ttccaacttt caagaatggc tattaggtaa
    26581 ttgaaactag gtttgtgtga tccggaagtt ttctcgactg cacggttcaa catatcgaaa
    26641 ctcattccat caagtcccac ataatcaagt aactgttgat gtgacttatc agttagatgg
    26701 attccgctat ttttcaaatt ttcagaaagg ctggaactaa tcatcacctt attattattt
    26761 atctctatat ctttatctaa ttctttatct agtgcgttac catgcgttac tgtaacgtta
    26821 tccataacgt tatccataac gttatcagta acgttacttt cgttgttctg tgcaagtaat
    26881 ttctgttttt gtcggtgacg tgctgctcta tttcgatttt gctcttttat tttttccatg
    26941 ccgtcaatgt tctgatgttt ctcccaattg ggtaacatta tcactccatc gattttttca
    27001 accattccaa acttttcaaa aacttcaaga gccatacgaa ctgtattcaa cggacggtgg
    27061 aacgtttgtg caagcatttc atcggtgtat acaatgtttt tagaaatggt tagtacgcct
    27121 tttgtattca atttgcctgc tagtgttata agtttgatcc agattacaat aattgcatca
    27181 cgatcaggcg aggcatcaat tagacacatc ttttcatcgt caaaaatatc cgttttgatt
    27241 tttatccact tgatttcaga cataccgagc accccacttc ctgcgattgg cacgatactt
    27301 catccgcata tcttcataga tgtacctgcc ttccagctcc attttttcaa tctttagcag
    27361 cttattttta agggtcatat aacgatagtc ctttgctagt ttttcatagt cggttaggta
    27421 ttctttgact cgtaatagat ttttataatc gttttcccat atcgtaataa aatgtcttga
    27481 agttgattcc cttccttcca gttctttaac aatcataatc aggttatcca gcgattcaat
    27541 caattcttgc atttcctgac ctcctcatta caaaaatctg attgcagact gtttaggttc
    27601 tggcaaagct aacggctcag gacgcaagcc tacaggcggt tcgttgtcat atgtgaatcc
    27661 tttgaactct ctgcgaatat tcttgcgaat ttcttgccat ttgtcctctc taccacgttc
    27721 atatgcatga ttacgcactt ggataatcat agacgcaaat tcttgctctt ctcgtctttc
    27781 ttcttccttg cgttgttcct gcaatttgat atgacggcaa gcccctgcaa atccaagcag
    27841 taaggctcca acccccatca gctggtctaa aatcggtggt tcaaacattt ttatctcctt
    27901 atcctctttt tgtgctataa tatagtcaaa taattttgct aagaccttgt ccagaagcct
    27961 tttagtaaag ttattatatt tgattagaga gccattcttt gatggctctt tttgaccatt
    28021 tcttaccagg taattccttt ggaaatccct ttaagtaacg ataattatct gaaaaggtgg
    28081 catacttaat tcctagaaaa tcacaggtag tgttcacatc catcagctct ggatagtggt
    28141 cactatcttt ttctatttcg actagccttg tgattgtgtc cttgataatg gatttaatcc
    28201 attcagacag tgaaagtaga acattgtcca tcttcttccc ctcctaccct tcgtcaaatg
    28261 agttcaattt catgattttc atcttggtat tggtgcttgg ctcccaagtc atccaatagg
    28321 ccaaggctgc atctgcgaat tttttcggta gcaagtcata gcgattaatg ttgaagtggt
    28381 ctttaaagtc aatctcagct tgtctaaaga ctgactgagc gaaagtctta tccgcataag
    28441 ccggactatc aatgccacct aagcaaacca cgacccgagc cttacgcttc ttcaggagcg
    28501 atttagcata gcttgggtga atcggttgct cactcttgag atagtcaata tcttccagca
    28561 tggtcgcctg ttgctcacgt aatttcttct ggccagtaaa cagagcgata aaggcatcct
    28621 cgtccaagtc ctcgcgaatg aaaccgccct gcttgcgaat agctggcaaa acctctgatg
    28681 tcacccagcg cttaaactct ttagcctgag gcaacttgct ggataagata agagagtaga
    28741 ggccagattc gttgatgatg atagtttctt gaacccttcc taaattatct gtgaggccct
    28801 gttttagggc gtcatcttca tcaacatgaa gagcaatcgc atttctagcc ttgctatatc
    28861 ctaggatgtc tgcaacatct ttcccaacga accaaggttc gtcatcaatg atcaaagtac
    28921 ggacttcctg cccgtgaaaa ttaaaaattt cgttcataaa gttcctttct aaatttggta
    28981 taattaaaat aaaacgattg gagaaatctt atggaatatc aagctcttat tcaacctgtg
    29041 attagtatta ttcttgcaat aatatcaggg ttatggtcat atgtcgcatc aaaagctaac
    29101 aataaagctg agattgaaaa acaagccaaa gaacattcac atactgttga aaaactcgaa
    29161 agagaatttc atcatcagat agatactcta aaacagcaac atgcctttga acttgaaaaa
    29221 atcaaacaag ctcatgaatt acggttgcaa gaacttgaga aggtatctca gcttgatact
    29281 gaaaccgaca agtctatgaa gatgaatgat cttacctaca aagtttttac aggcgaggtt
    29341 gattttgaca aagctttaaa aatagctaat aaagcaagca atagcaaaca aaacttaaat
    29401 aaaagattta ttcaaaagac ttctaaaaaa tcataaatta aatttatttc taactctttc
    29461 aagctcatca tcttgtattt ttttaaaccc gttgatgcgc ttttctctat ctcttttgct
    29521 agcgtagtat gtagcaaaac caatgataag attgattatg acaacaaagt aaaaccatac
    29581 tagttcattc atctccaccc tcctactcca gcaccttact gccgactacc aatcgtttaa
    29641 cgacaacgtc catctcctta aattcggcat tctctgcaca gtagcggacg ctctcgctga
    29701 tgatgtgaca aatagatacg ccgtactcgt tcgccagctc cgtagcaatc tcccaggcat
    29761 ctttgtcaat ccgtgttact ttttgcgctg cgttgttcat actatttcct catgctctaa
    29821 ctattttccc aagggttttc aatacccaaa atatctacga ctttttcttt cacataatca
    29881 cttccttttc catacttcag caactctgaa ataactgatg atgctacaga tacttgtttt
    29941 gccaattcga cttgagtcat atccaactca atcaaacgag ttttaatttt agccttaatt
    30001 atttttagtt ctttactcat tttttcctcc aatctgtgtt ttagtatttt ctagatatat
    30061 tgttaggatt tgaactatgt cctctagtga atatgttacg ggcttaaaga taaaatgttt
    30121 ttcaaataat cttattgcct catagtgatg tctacttgtt atatatgaat tattttcaat
    30181 aaaaaattta cagaattctt ctgacgattt gtctgtcata caaaataata gacaaagtga
    30241 tagagtttca tctttattgt tttttatttt cgaaaagtca tcgaagagat ttctgccaaa
    30301 cataacttta tacctgactg gaacatctgc ccttgcagat atgaagtatg tctttttgtt
    30361 tatttttatt tccagtgttt ctatcatttc ctacctcgtt tacctacttt atgtgactaa
    30421 taattttgct ttactcatat ttttcctttc tatatttctc tgcttctttt gaaattttca
    30481 aaagcattga aagtccccca actactccat ctagataccc tcgcccataa tctgtcgcta
    30541 agagttccaa taattctttc aggtcttctt cgttcatccc tgacctcctt ttaaaaaatt
    30601 atctaaaaag ttagcgaact tcttgacaac tctagtcaaa tgttttaaaa taaaaacata
    30661 gagaaaagac ctactaaaag taagttttac ctacagtaaa cggacgccaa tcagttttgt
    30721 aaggctttat tttttagttg tctagttcgc taactcttta gcttacgaat attattttaa
    30781 aatatttgac taaataagtc aactattttc tacaaatatt ttaaaattat ttttcgtttg
    30841 cttagaaagg ttgaaaaatt aatgtttgta gcattcgata aaataaaaga attagctgat
    30901 aaacagggga tttctataaa tattttagaa gaaaaacttg gttatggaac taatacttta
    30961 tatcgattaa aaagaagtaa tcctagttca aaagttttaa aagaaatagc tgattatttt
    31021 aatgtaagtg ctgactacct actcggacgg acagataatc ctgctatcgc tggaagtgat
    31081 gaatttgctc aagtaaatgg acaaatcata gacttacgca aagcagcagc caacaccatg
    31141 ttatttgacg gaaaaccact aaatgaagat gatatcgact tcatcacatc cgttctatcc
    31201 gcccacttca aaagcaaagg agaacgctaa tgactatcac gatcaatttc acagaaaaaa
    31261 actcctacat cacggactac ctaaccaaac acggtatcga cacaacaacc atggattttg
    31321 acgacttcat ggtactcatg gaagatatcg aagacgcacg agcagccgac caagcctata
    31381 tggagtattt agccgaccca gctacttata ccatggatga ggtcttggat gaactaggac
    31441 tatctcgaga ggatattgct taatgtatcg gctagatatt gataaaaaag ctctcaagca
    31501 acttaaaaaa ctagataccc caaccagaaa acaaatccta tcctggcttg ctaaaaacat
    31561 tgaaaacacg accaatccac gacaacacgg aaaagcacta aaagccaacc ttgcaggtta
    31621 ctggcgatac agagtagaga attaccgcat catctgtgat atccaagacg ataaactagt
    31681 cgtcctagcc gtggaaatcg cccaccgcag agatgtttat aaataacgaa ggagaactat
    31741 gatactcgct aaactctgcg aagaatatca agtagagctt tgtctcttcg acggttcaaa
    31801 ctggcacagt agcggtttct acaatccaga cacaaacgta ctcgctattg accacaactt
    31861 agctcctgaa caacaaatcc aagtcgccct acactaactc ggacacaaag accatacacg
    31921 ctcagagtac caaaacgctc gcctacgctg tgaaaacgaa gctgatagga atatgatcca
    31981 tcatctcgtg aaagacgcac tagaaagctt agacgacccc acagagtttg attacctcaa
    32041 attcatgtct tactacaatc taaaaactat gactaatgaa gtcatggtca aggaagagta
    32101 cttggcatta gtaaattaaa aattgcattg aagatattta tatagagggg aaatatatgc
    32161 aaaagactgt tgaaaaaata ttattcagag tcgctggagt gacaaaatat aaaaaagcag
    32221 taaaagaagc ttgcaatatg attgctgaag ataacggaat cccagaatat gcaaagtact
    32281 atagcaattt atcaactaag gaacttagag aagaacttga agaatatggt ctgaaagttt
    32341 tcaaatatca agatttagac tttttcaata ttgagctcgt tccagaagta gataatagat
    32401 atgatcctaa tgctataaaa gttttgatat ttgataatca tgtaggatat gttcctgcaa
    32461 ccattgctaa aacaatacga aaatattttg ataataaaaa atataacttt gtaatagagg
    32521 gagagataaa aggtggccca tataaagaat gggacgagta tgaagaaaaa gttgtcacaa
    32581 ataatgattt ggatgttggt tttgaaattt accttaccat tgttgattct tcacaaaaag
    32641 aagtaattca gagcgaatca tctgagataa ttgatgataa catttccaat aaagaagtta
    32701 cggaaactga agctattgaa actaaaacaa ctgaaactga acatattgaa caaaatattg
    32761 ttagtgacag tgtcgctgat atagtaaacg agattaatct ttcagaaaca tctcctaaga
    32821 aaaaacttcc tgccaataaa atcatatttt cagcactgta tcttttctta gtcttttttg
    32881 gagttgttgg tattccaatc gctccattcc ttgcagtccc tttgacagct tggagtttgt
    32941 ataaactata caaactattc agaaaataaa aaaaaagccc cacaatcgcc ctcgccaaag
    33001 tttgattgtg aagcttaccc ttataaaaaa tcagccatta aaaaggcctc ttttctatac
    33061 cctattttac accatgaaag gggtgatgtc aatattctca atgtttagac cttgtccaga
    33121 agccgataaa caaggagaat acaatgaaat ataataaaac aaaataccca aatatctatt
    33181 actatgagac tgctaaaggc aagcgttact atgtcagacg ttcttttttc ttccgaggta
    33241 aaaaaagaga aaaaagtaaa agtggtttca caactctccc tcaagctcgt gcagccttgg
    33301 tagagcttga gcaacaaatc caagaacaag aattaggtat caatacgaat ctaacgcttg
    33361 atcaatattg ggatatctat tctgaaaaga gattgtcaac agggcgctgg aatgacactt
    33421 cctactacct caatgacaat ctctataaga accatatcaa ggcaaagttt ggttctatcc
    33481 tgcttaaaaa tttggataga aatgagtatg aactatttat cgctgaaaag ttgcagaacc
    33541 ataccagata cactgttcaa accctcaatt ccagcttcat ggcattgctg aatgatgccg
    33601 tcaaaaatgg aaatctgctc tcaaatcgct tgaaaggtgt tttcatcggc cagagtgata
    33661 tccctgctgc aaacaagaaa gtgactctca aagagttcaa gacttggata gcaaaggcag
    33721 aagagattat gccaaaacaa ttctacgctc tgacctatct gacaattttt ggattgagaa
    33781 gaggagaagt ctttggattg cgtccaatgg acatcactca gaacgacagc ggacgggcta
    33841 tactgcatct tagagacagt cgaagcaacc agaccttaaa agggaaagga gggcttaaaa
    33901 cgaaggattc agagcgatat gtctgccttg atgatatcgg aacagacctg atctattatc
    33961 tgatagctga agcttctaag attaagcgaa agttaggaat tatcaaggaa cagcacaagg
    34021 attatataac tatcaacgag aaaggtggtc tcatcaatcc aaatcagcta aatagaaact
    34081 tcaatctagt gaatgaagca acaggattgc atgtaacacc tcacatgatg cgccacttct
    34141 tcacgactca aagcattatt gcaggggttc cgcttgaaca attaagccag gcgctggggc
    34201 atacaaaggt ttatatgacg gatcgttaca atcaagtaga ggacgaactt gctgaagcga
    34261 caacagacct atttcttagt catattcgct aaaaaagtcc ccgccaattc cccgaccaaa
    34321 a
//
LOCUS       DAVBDG010000019        32413 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBDG010000019 DAVBDG010000000
VERSION     DAVBDG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.019"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            181..519
                     /locus_tag="VQ526_000866"
     CDS             181..519
                     /locus_tag="VQ526_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2554607.1"
                     /translation="MTLTINVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            709..2145
                     /locus_tag="VQ526_000867"
     CDS             709..2145
                     /locus_tag="VQ526_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2554608.1"
                     /translation="MTTFKDGFLWGGVVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSLAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEIV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTVQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            2197..2436
                     /locus_tag="VQ526_000868"
     CDS             2197..2436
                     /locus_tag="VQ526_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2554609.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLLGIILFYKLKYK"
     gene            2566..2880
                     /locus_tag="VQ526_000869"
     CDS             2566..2880
                     /locus_tag="VQ526_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2554610.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            3004..3213
                     /locus_tag="VQ526_000870"
     CDS             3004..3213
                     /locus_tag="VQ526_000870"
                     /inference="COORDINATES: protein motif:HMM:NF019884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2554611.1"
                     /translation="MLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLKLI
                     EKTMIIQGVRLISKQGQGYQIFFEN"
     gene            3415..4977
                     /locus_tag="VQ526_000871"
     CDS             3415..4977
                     /locus_tag="VQ526_000871"
                     /inference="COORDINATES: protein motif:HMM:NF013070.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2554612.1"
                     /translation="MLSEYNLSIESRANKGVYVSGEERDKRRFIINYFLENQFFKTIH
                     CYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDFVLQNLVVHIALSVIRLKSGFEIK
                     NIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQEIDYITLHLLAKSQQCQKNQKNIS
                     EEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLITHLMTLQVRLESRITLNNPLVDEI
                     KQNYSDIFFMTREILANMDKFLEWTISDDEVAYVSLHFLAAMERSKESTKFNILAICA
                     TGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQEKLKGIDFIVSAVDLSNLYFQIPV
                     FKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINKFENNGFRQYFSKENFLICTESDK
                     VNLLEKMVESLSVGESNEFEQSLLYGIKQREELSSVVFSEKIAVPHPIQPFGTEGKVS
                     VAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLATVTKKIVSLTENDELQNQLISCNN
                     FEDFINIFEKIK"
     gene            4987..5301
                     /locus_tag="VQ526_000872"
     CDS             4987..5301
                     /locus_tag="VQ526_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2554613.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5332..5838
                     /locus_tag="VQ526_000873"
     CDS             5332..5838
                     /locus_tag="VQ526_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554614.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5918..7264
                     /gene="celB"
                     /locus_tag="VQ526_000874"
     CDS             5918..7264
                     /gene="celB"
                     /locus_tag="VQ526_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2554615.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7643..7819
                     /locus_tag="VQ526_000875"
     CDS             7643..7819
                     /locus_tag="VQ526_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554616.1"
                     /translation="MSLDIDKEKMTIMGIAVENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8220..10433
                     /locus_tag="VQ526_000876"
     CDS             8220..10433
                     /locus_tag="VQ526_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET2554617.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHTTDIPARFGSGWTGYSWNRSLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10655..11131)
                     /locus_tag="VQ526_000877"
     CDS             complement(10655..11131)
                     /locus_tag="VQ526_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2554618.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFFIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11354..14557
                     /locus_tag="VQ526_000878"
     CDS             11354..14557
                     /locus_tag="VQ526_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2554619.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSSWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEVKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKGGTTNVIVSKDGKE
                     VKKIPLKILASVKDTYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTVKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            14941..15326
                     /gene="tnpA"
                     /locus_tag="VQ526_000879"
                     /pseudo
     CDS             14941..15326
                     /gene="tnpA"
                     /locus_tag="VQ526_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15705..16334)
                     /locus_tag="VQ526_000880"
     CDS             complement(15705..16334)
                     /locus_tag="VQ526_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2554620.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(16344..17345)
                     /locus_tag="VQ526_000881"
     CDS             complement(16344..17345)
                     /locus_tag="VQ526_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2554621.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(17376..18017)
                     /locus_tag="VQ526_000882"
     CDS             complement(17376..18017)
                     /locus_tag="VQ526_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2554622.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(18036..18851)
                     /locus_tag="VQ526_000883"
     CDS             complement(18036..18851)
                     /locus_tag="VQ526_000883"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2554623.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            19118..19552
                     /locus_tag="VQ526_000884"
     CDS             19118..19552
                     /locus_tag="VQ526_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2554624.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTVNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            19564..20754
                     /locus_tag="VQ526_000885"
     CDS             19564..20754
                     /locus_tag="VQ526_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2554625.1"
                     /translation="MIKKVTIEEIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELAHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            20765..21256
                     /locus_tag="VQ526_000886"
     CDS             20765..21256
                     /locus_tag="VQ526_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2554626.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            21271..22050
                     /locus_tag="VQ526_000887"
     CDS             21271..22050
                     /locus_tag="VQ526_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2554627.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            22037..22855
                     /locus_tag="VQ526_000888"
     CDS             22037..22855
                     /locus_tag="VQ526_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2554628.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            22855..23148
                     /locus_tag="VQ526_000889"
     CDS             22855..23148
                     /locus_tag="VQ526_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2554629.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            23170..25071
                     /locus_tag="VQ526_000890"
     CDS             23170..25071
                     /locus_tag="VQ526_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2554630.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALIQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            25131..26132
                     /locus_tag="VQ526_000891"
     CDS             25131..26132
                     /locus_tag="VQ526_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2554631.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(26242..26577)
                     /locus_tag="VQ526_000892"
     CDS             complement(26242..26577)
                     /locus_tag="VQ526_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554632.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLSNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(26588..26830)
                     /locus_tag="VQ526_000893"
     CDS             complement(26588..26830)
                     /locus_tag="VQ526_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554633.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(26841..27383)
                     /locus_tag="VQ526_000894"
     CDS             complement(26841..27383)
                     /locus_tag="VQ526_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2554634.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYLGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(27399..27593)
                     /locus_tag="VQ526_000895"
     CDS             complement(27399..27593)
                     /locus_tag="VQ526_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2554635.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            27759..28709
                     /gene="rsmH"
                     /locus_tag="VQ526_000896"
     CDS             27759..28709
                     /gene="rsmH"
                     /locus_tag="VQ526_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2554636.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            28721..29038
                     /gene="ftsL"
                     /locus_tag="VQ526_000897"
     CDS             28721..29038
                     /gene="ftsL"
                     /locus_tag="VQ526_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2554637.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            29042..31294
                     /gene="pbpX"
                     /locus_tag="VQ526_000898"
     CDS             29042..31294
                     /gene="pbpX"
                     /locus_tag="VQ526_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2554638.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            31296..32276
                     /locus_tag="VQ526_000899"
     CDS             31296..32276
                     /locus_tag="VQ526_000899"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2554639.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGIIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
BASE COUNT        10202 a         5326 c         6975 g         9910 t
ORIGIN      
        1 tttgtcctgc tcttgtttca attgactata aatcgctata agaaaaaatc attttattgg
       61 tataaagaag tgattgaaag taatggcgaa acgttagata attagttcat aaaaaacaag
      121 tttagatcct catctaaact tgttttttta aaattgaagg atgattttga aggatataag
      181 atgacattga ctattaatgt atattataaa ggtgatggag atagtgcaat aaagtttgca
      241 agagaaatgc ttgatagtgg ccttgttgaa gaaattagaa atcaaaaagg taacttgaaa
      301 tacgaatatt ttttaccaat tgaaaaagag ggaactattc tccttattga tcagtggata
      361 aatcaagaaa ctttagataa gcatcatcaa tcaaagacaa tgcagaaaat cttagattta
      421 agaaaaaaat atcacttgca gatgcaagtt gaacgttata tagaagatga ttctgggatg
      481 ccagaaagcg ataagtcgtt tatagacaca gggaattaga attattgtga gagtttttgt
      541 agtttgaaag tattgaaaat cgtaaagata tcattacgag aatacaagaa gatattgttc
      601 tatttccatt aaaaattact tctttcctta acaataagga aaaagaatgc gttttcatct
      661 tttggtttga ggtttataat aagagtatca aagtcaggag gaaaaaagat gactactttt
      721 aaagatggat ttttatgggg tggtgttgtt gctgctcatc aacttgaagg tggatggcaa
      781 gaaggtggca agggaattag tgttgctgat gttatgactg ctggtcgtca tggagtagct
      841 cgtgaaatta ctttgggagt tttagagggt aaatattatc caaatcatga ggcgatagat
      901 ttttatcatc gttataaaga agacatagca ctttttgctg aaatgggatt caagtgcttc
      961 cgtacctctc ttgcatggac acgtatcttt ccaaaaggtg atgagttaga gccgaatgaa
     1021 gaaggattac agttttatga taatcttttt gatgaatgct taaagaatgg tattgaacct
     1081 gtcatcactc tatctcattt tgaaatgcct tatcacttag tgaccgaata tggtggttgg
     1141 aaaaatagga aattgattga tttctttgct cgttttgcag aaatcgtatt taaacgttac
     1201 aaagataagg ttaaatattg gatgactttc aatgaaatca ataatcaagc gaattatcag
     1261 gaagattttg caccatttac taactcaggt attgtatatg aggaaggtga taatagagag
     1321 gcaattatgt atcaagcagc acattacgaa ttagttgctt ctgcacgagc tgtaaaaatt
     1381 ggtcatgaga ttaatccaga ttttcaaata ggttgtatga ttgcgatgtg tccaatttat
     1441 ccagctactt gcaatcctaa ggatatctta atggcaatga aagctatgca aaagcgttat
     1501 tattttgctg atgtgcatgt tttaggtaaa tatcctgagc atattttcaa gtattgggaa
     1561 cgaaaaggta tttcagttga ttttactgtc caggataaag aagatttact tggtgggact
     1621 gtagattaca ttggtttcag ttactatatg tcctttgcta tcgactctca tcgtgaaaat
     1681 aatccttatt ttgattatct tgaaacagaa gatttagtga aaaataatta tgttaaggct
     1741 tctgaatggg agtggcaaat tgatccagaa ggtttacgtt atgcgttaaa ttggtttaca
     1801 gaccactatc acttaccact ctttattgtt gaaaatggtt ttggagctat agatcaagtt
     1861 gcagcagatg gtatggtaca tgatgattat agaattgaat atctaggtgc ccatattcgt
     1921 gaaatgaaaa aggctgtagt tgaagatggt gttgatttaa tgggttatac tccatgggga
     1981 tgtattgatt tggtttcagc tggtaccggt gaaatgcgga aacgttatgg ctttatttat
     2041 gtagataaag atgacaatgg gaagggaagt tataatcgtt ccccgaaaaa atcttttggc
     2101 tggtataagg aagttatttc atctaacggt gaatcagtag aatagtataa acaaactgag
     2161 attattctca gtttgtttag catattgagg tgacatatga ttggaagtat tgcagcaatt
     2221 ttaacaacat ttgcattttt accacaagtt tttcgtgtag ttaagacaaa ggacacagga
     2281 tctattgctc taggtatgta tgttatgcaa gttattggta ttgcattgtg gcttgctcat
     2341 ggtattcgta taggagactt gcctttgatt ttagcaaata gtgtttcatt tctactctta
     2401 ggaataattt tattttataa attgaagtat aaataatttt tttccatatc atttgggaaa
     2461 atgagggaat tgtcattgaa aacgctttat tttataataa aaatagataa caaataagcc
     2521 aacttagcac agctttgatt ggatttaaga aaaggagaaa atattatggc aaaagcttta
     2581 attatttgtg cagcaggaat gtcttcatca cttatggcac agaaaacaac agagtttcta
     2641 aaaggaaaag ggaaagatat tgtagttgat gctattagtg ctactgaagg agggaaagct
     2701 atcgcagcag cagcttttga tttgtatctt gtcagtcctc aaacaaaaat gtactttaag
     2761 caatttgaag aggctggttc taaagcagga aaaccagttg tacaaatacc tcctcaagcc
     2821 tatattccta ttccaatggg aattgaaaaa atggctaatt tgattcttga gaatatttga
     2881 tgaacacatg aaatgtcaat aaatatttaa gttaagataa attgttttga gtttgatata
     2941 aattgattta agtgtataat atgcttcaat atatgaaaac atacgtttta ggagggaatg
     3001 aatgtgttaa acattaaaga gaaagacatc cttcaatttt taataaaaaa taaggaacgt
     3061 tttgttacaa gcaaagaact ggcagaatat ttatcctgtt cagatagaac tgtccgtaat
     3121 gtactgaaat taattgagaa gacaatgatt attcaaggag tccggttaat ttcaaaacaa
     3181 ggtcaagggt atcaaatatt ttttgagaat taaggcgctt accaggagtt tagacaaact
     3241 tatgaactgg aagaagacta tacgaaaact gcggtttcaa aaggtgatga tcgtttagta
     3301 tttatcttaa ataaattatt atttgagcaa gttccagttc tttttgatga tttagctgat
     3361 gaactttacg ttagtcgttc aacattatct catgatttca gaaagatacg agtgatgtta
     3421 tcagagtata atttgtccat tgagagtagg gcgaataaag gagtttatgt ctcaggcgag
     3481 gaacgtgata aacgacgttt tatcataaat tatttcctag agaatcaatt ttttaaaaca
     3541 atacattgct atgtaaaatt taactttttt gatcaaactg ttccattaga agaatttgct
     3601 agaattgttt tggatgaatg ccaagaggcc aacctaaaac tatctgattt tgtactacag
     3661 aatttagtag ttcacatagc attgtctgtg attcgtttaa aatccggatt tgaaataaaa
     3721 aatatagact gtcagatgac tgatgatgca atagagagaa aggttgccca aagaattctt
     3781 tctaaagtaa gagaagtaac aaatcaggaa tttccagttc aggaaataga ctacattact
     3841 ttgcatttgt tagctaagag tcaacaatgt caaaaaaatc agaagaatat ttctgaagaa
     3901 gttttaaaaa aatctttatt taaaacattt cagaatttag gattagatga tatgtataac
     3961 ttttcttcag atttccaact aattgaagga ttgataactc atctaatgac actacaagta
     4021 cgtttagaaa gccggattac cttaaataac cctttggtag atgaaattaa acagaattat
     4081 agtgatatat tctttatgac tagagaaatt cttgcaaata tggacaagtt cttggagtgg
     4141 acaatatcag atgatgaagt agcatatgta agtcttcact ttttagctgc tatggaacgt
     4201 agcaaagaga gtactaagtt taatattctt gctatttgtg caacaggatt tggtgcagcc
     4261 caaatgttaa gaaatcgttt ggaaacggag tttgggaagc gagtagaagt tgttgatgta
     4321 ataggctatt atgaattgaa tcaagaaaaa cttaaaggaa tagattttat agtgtcagct
     4381 gttgatttat caaatctata ttttcaaatc ccagttttta aagttagtgt gtttcttaaa
     4441 agtgatgaga tggaaatgat tagaaaagct atggatcaga tgcaagtttc tagttatgta
     4501 caatcatcaa agattaacaa atttgaaaac aacggcttta gacagtattt tagtaaagaa
     4561 aactttttaa tatgtactga atcagacaag gtaaatttac ttgaaaagat ggttgagagt
     4621 ttatctgttg gtgaatcaaa tgagtttgaa caatctttac tttatggaat taaacagcgc
     4681 gaagaattaa gttcagttgt tttttctgaa aaaattgcag ttccgcatcc tattcagccc
     4741 tttggtacag agggaaaggt ttcggttgca atttgtaaag attcgttact ctgggataat
     4801 caaagttcac atgtccagct agtatttctt ctatctccat caatatatgg gaacgaaggt
     4861 ttggcaactg tgactaaaaa gatagtctct ctgacagaga atgatgaatt gcaaaatcag
     4921 ctaatatctt gcaataactt tgaagatttt attaacatat ttgagaagat aaaataggag
     4981 gttcatatga atcaagagga actgcaggtt gcagcatttg aaattatcct gcattcggga
     5041 aatgcaaggt cagaaataca tgaagcattt gcaaaaatga gagaaggtag ttttgatgat
     5101 gcagaaagta aattaaatca atcaaatgag ataattcttg aagcgcatca tgcacaaacc
     5161 aaactcttgc aagaatacgc aagtggagtg gaaattaaaa ttgaaattat catggtacat
     5221 gctcaagatc atttaatgac cactatgaca ttattagaag tagcaaagga aatgttagct
     5281 ctttataaga aagttaacta aatatgtgtt gttatataag gaggaaatga tatggataaa
     5341 aaagaacgac aaaagattga acaacaacgt cgagagatgg ctctaactaa tacttttttt
     5401 aatcgatatc ttcttctacg ttattcaata gcactcttct tttttgggaa tatttattgg
     5461 cttttgaatc agtttattag tccctcgcct attattattt tccccattat gctcatagta
     5521 ttttctattt tagcaactat tgaacaattt aaactttatg gaaataggaa agaaaagttg
     5581 ggaataacac taatgtttgt tagaatacaa atacttattt caataggatt acttgtttta
     5641 acttggacaa gttggtttaa gaatcttttt ccgatttttg aaaataacca agtagcacgt
     5701 ttatttgttt ttgtagttct tctattaggt ttagttctga gtttgttaga cattagaaga
     5761 attaaaaaaa tttataaacg aacagataag gcttatcaac aatttgtcca attggaaaag
     5821 aactcactta gtttataatc ctgatctagt ataatttgta tttgaaacta gtctttagaa
     5881 agtaaaaatt attatgtttt attaaggagg tctatttatg gatcaacaaa acgggttgtt
     5941 tggttttctt gaaaaccatg ttatgggacc aatgggcaaa cttgctcagt ttaaagtagt
     6001 gcgtgctatc acggctgcag gtatggctgc tgtaccattt actattgtag gatcaatgtt
     6061 tttggtattc agtattttgc cacaagcttt ctcattttgg ccaattgtgg cagatatttt
     6121 ctctgcttca tttgataaat tcacatcact ttacatggtt gcaaactatg cgactatggg
     6181 ttctctatct ctttatttcg ttctatcact tgcatatgaa ttgacaaaaa tttatgcaga
     6241 ggaagaagaa ctcaatatga atcctcttaa tggtgccttg ctcgccttga tggcttttgt
     6301 catgacagta ccgcaaatca tttttgatgg tggaatgatg aagactgtga caagtctaaa
     6361 agaaggtgca gtaattgcag atggatgggc aatgggaaat ggagtcgcac gttttgggac
     6421 aacagggatt tttaccgcaa tcattatggc aattgtgact gttcttattt atcgtatgtg
     6481 tgttaaacat aattgggtta ttaaaatgcc tgaagctgtt ccagaaggag tttctcgtgg
     6541 atttaccgct ttggttccgg gatttgttgt tgcatttgtt gttatcttta tcaacggtct
     6601 tcttgtagca atgggaacag atatttttaa agtcattgca attccatttg gttttgtatc
     6661 caatctgact aattcgtgga ttggtttaat gattatttat ctattgactc aactactttg
     6721 gattgtaggt atccacggtg cgaacattgt ttttgcattt gttagtccaa ttgctcttgc
     6781 taacatggct gaaaatgctg ctggcgggca cttcgctgtt gcaggtgaat tttctaatat
     6841 gtttgtaatt gcaggtggtt ctggtgcaac tttaggacta tgtttatata ttgcttttgc
     6901 ctctaaatct gaacagctta aagcaatagg acgagcatct gtagttccag ccttatttaa
     6961 tattaatgaa ccattaattt ttggattacc tattatctat aatccagcct tggctatacc
     7021 atttatttta gcaccaatgg ttactgctac tatttattac gtagcgaatt ctctaaactt
     7081 tattaagcca attatcgcac aggttccatg gccaactcca gtagggattg gagctttctt
     7141 agggacagca gatcttcgag ctgtattagt tgctctagta tgtgcatttg cagcattctt
     7201 agtctatctt ccattcatcc gtgtatatga tcaaaaattg gtgaaagaag agcaaggtat
     7261 ctaataagta aaaaagtata ttcgatgata gaatccatat agtttaaata atgagtgtta
     7321 atattagcag tgaagaggtc tttttaagat attcttctct gctttttcat attttttcgc
     7381 tatgtagggt atgaagcaaa atagaaagta gttcgaatag ttgataaaca ggaaactgct
     7441 gttatttaaa aaaacaataa agtaaagtat gttatcgtag aatacgatac aatagtaaaa
     7501 aagcaaaaag aatgcgtttt atatttgggt ttgaggttta taaaatagga taagcgtgct
     7561 gaaaacttag atatgacaaa caaaataaaa tttttaaaag ctacatagaa acatggtata
     7621 atgtatctaa aggaggtagc atatgtcctt agatatagat aaagaaaaga tgacaatcat
     7681 gggaatagct gttgaaaacc gatccgtttt taagagtgtt tggtatgctt taagtacaaa
     7741 tatgattgaa ggatggcgac ctacggttag tgatgttgaa aaattacgag atgaagctct
     7801 tgctctgggg atgacttaat gaagcgcaac tgttatgaat cttatgacta tgttgatccg
     7861 gataatttat ataccgatcc gggttcttca gttctaagaa ataagcagga aggaaaaaag
     7921 cacgcgagct tgaatatcgt atgattgcta gtaagagttt gaaattattt atcaatccta
     7981 tcttagttaa tagtttaaaa gtagtttgtt tcacaactgc ataagcttag aaaagttgaa
     8041 aagtgataac tctttaataa attcttggtt tgttaaaaga tattttgtcc actacatttt
     8101 ttcaattgac tattatttgt catagatata acagatagaa cattgcattc agaataatga
     8161 aaaacgcatc aaaaatggtg cgttctgttt ttatgctacc ataaaaggag gaaaacaaat
     8221 tgaaaatttt taagggagag ttttatcgaa tctctgtatt aacagacaag ctagtaaggt
     8281 tagaatactc tcgaactgga aattttgagg atagaacgac acaacttatc tataatagag
     8341 attttggcca agtttcgtta gattatatcg agacatcaaa tgtactagat attatgacgg
     8401 actattttca tctgcacttt aataaaggag aatttaacgc cgaaaattta tttatagaat
     8461 taaaaggaaa ttttgccgta tatggtagtc gctggtattt tggtgaatct attgaaacgt
     8521 taaaaggaac agctcggact ctggataagg cagatggagc aatctcgtta gaagatggaa
     8581 ttattagccg aaatggtata gccttattgg atgattctca aggatttatt tgggatgaac
     8641 aatctggtta tattgagaga gaaaatcaaa ttgacctgta tttctttgcc tatgggcatg
     8701 attatagagg agcaatcaga gacttttacc atttgactgg ttcaacaccc ttgttgccaa
     8761 gatatgcttt aggcaattgg tggagtagat attggcctta tacgtcggat gaatacttgg
     8821 atttaataga cagatttgaa acagagaaaa ttccattatc tatcggtgtg ttagatatgg
     8881 attggcatac aactgacatt ccagctcgtt ttggaagtgg ctggacagga tatagttgga
     8941 atagaagctt aataccaaat ccagaacagt tattgcaaca acttcatgat agaaagttaa
     9001 aactctcctt aaatgtccat cctgctgatg ggatacgggc ttatgaagaa gcttatcctc
     9061 aaatcgcaaa acggttgggg ttaaatgtag aactagaaga acctgctatt tttgattttt
     9121 ttaatccctc ttttagggaa gcctacttta aagatgttca ttatgagcta gaaaagcagg
     9181 gagtagattt ttggtggatt gactggcaac aagggacaca aggtatgcta gatccacttt
     9241 ggcttttaaa ccattatcac tatcaggata gttgtaaaaa tgcagaaggt ggtttgattt
     9301 tatcaagata tgcaggtcct ggtagtcacc gctaccctgt tggtttttca ggggatacta
     9361 ttattagttg gaattcctta aggtttcaac cctattttac agcgacagca tctaatatcg
     9421 gttatagttg gtggagtcat gatatcggtg gacatatgtt gggggattat gacgaagagc
     9481 tacaaactag atggctacag tttggtgttt ttagtccgat tactcgatta catagttcta
     9541 gaagtccttt taatagtaaa gaaccttggt tcttttcaga aacaacatct aagattatga
     9601 agaaatacct tcgtttgaga catcagatga ttccctatct atataccatg aatgtaaaga
     9661 cacatgagga aggtgcccca ttaatcagtc caatctatta tttctatcca gagaatgatg
     9721 agagctataa tgttccaaac caatactttt ttggaacaga actgatggtg gctcccattg
     9781 tagaaaagat ggatttgaca ttccaatctg caaaagtaga tgtatggttc cctgaaggtg
     9841 aatggtatga cttcttttca gagaaaaaat acacaggtgg tgtgaagtta agtgtttata
     9901 gggacatctc gactacgcct gtgtttgcaa aaagtggtgc aatcattccc ttggttggtt
     9961 ctgagatagg tatgggtgtt gatttacctg aagttgtaga ttggtatgta ttcccaggaa
    10021 aacaacattc ttttgaaatg cttgaagatc aaaatggtca aagatataaa acaagattat
    10081 caatcgattg ggaaatggga atggtagagt tagcattaca aggagattct agtatcgttc
    10141 caagcaatag aaaacataga attcatttta aaggaacgaa tgtgtctata attgaattgc
    10201 caaataagaa tgatacagct aaatttgaat ggaaagataa taaaaggaca tctttaaatg
    10261 acgaagtttt tagactacta aagacagctt ctcttccata tgaattaaaa gatagattgt
    10321 taaatcaatt catcaatgcc aaaaactctc atgacttaat gaatatcttg catcatcagg
    10381 ataaggaatt gagagggcgt ttgttggaaa tgatatttac tagccaaaac taattgatat
    10441 aattagaaag tcatttgttt agtgtatatg aagattctgt atggtattcg ttatcactgg
    10501 tcacattgaa aatgtgaaat aagcacctac aacatagact ttacaacact tcatgtttca
    10561 tatcatgggg tgtttttcta tttaagtcaa agccaatcaa aaaatctcac tattcatcag
    10621 gttaaaggaa acctaatcat agtgggattg tgaattatag tagagtttgt atcgcctctt
    10681 caatttgttt tgggtctgtt tttgaagaaa agcgttcaaa gacttgccca tcacgaccaa
    10741 tgaaaaactt agcgaaattc cattcgattc gttttcctag tgggccagat ttctggtctt
    10801 ttaaccagac atagagaggg tctgcttcct taccgttgac cttaatcttg gcaaaacgtg
    10861 ggaaggtggt ttgaaaatgt aggctacaga aggcgttgat ttcctctgcg ctgccgggtg
    10921 cttgtcccat aaactgattg caagggaaat ccaatatttc gaagccctga tcttgatagc
    10981 gttcatagag ttcttgaagt ccctggtact ggggcgttaa accacatcca gtagcagtgt
    11041 tgacaatcaa gagaactttg ccacgatagc tatccaaggg agttgcttgg ttgttttggt
    11101 tcaaaacgga aaaatcataa agtgaagtca tgagggcctc ttttcttttt ctacattgta
    11161 acagttttta cttttttcgt cgagtcggat ggaagatatt gttgaagaat aagccctcaa
    11221 tatgatagaa atcgtttaca aataacttta aaaatggtat gatttaacta aaccggttta
    11281 cttggttgct ataaatctct aatttgagat ttagttccta ttgattttac aatatgttta
    11341 ttggagtgta tacatgcaaa caaaaacaaa gaagctcatt gtgagtttgt cttcacttgt
    11401 tttatcagga tttttattaa accattatat gacagttgga gcggaagaaa cgactacgaa
    11461 taccattcag caaagccaga aggaagttca gtatcagcaa agggatacaa aaaatttagt
    11521 tgaaaatggt gattttggtc agacggagga cggaagcagt tcgtggacag gaagcaaagc
    11581 tcaggggtgg tcagcttggg tagaccagaa gaatagttct gcagatgcct caactcgagt
    11641 cattgaggct aaggatgggg ctatcactat ctcaagccat gagaaattaa gggcagcgct
    11701 tcaccgtatg gttcctattg aagttaagaa aaagtataaa ctgcgtttca agattaaaac
    11761 agataataaa gtcgggattg ccaaagttcg tatcattgag gaaagtggta aggacaagcg
    11821 attgtggaat tctgcaacga cgtcaggaac aaaggactgg cagaccattg aagcagacta
    11881 tagcccgact ttagatgttg ataaaatcaa gctggagtta ttctatgaaa caggaactgg
    11941 gactgtttcc tttaaggata ttgagctggt agaggtagca gcccagcttt ctgaggattc
    12001 tcaaacagat aaacagcttg aggaaaagat tgatttacca attggaaaaa aacatgtttt
    12061 ttctcttgcg gactatactt ataaggtaga aaatcctgac gttgcttcag tcaaaaatgg
    12121 aattttagaa cctcttaagg gagggacaac caatgtcatt gtcagtaaag atggcaagga
    12181 agtgaaaaag attcctttga agattctagc ctctgttaag gatacataca cagaccgttt
    12241 ggatgactgg aatggcatca ttgctgggaa tcaatactat gattctaaaa atgaacagat
    12301 ggccaaatta aaccaggaat tggaaggaaa ggtagctgat agcctatcca gtatttcaag
    12361 tcaggcggac cgcacctatt tgtgggaaaa attttcaaat tataaaacgt ctgcaaatct
    12421 gactgccact tatcggaaat tggaggagat ggccaagcaa gtgaccaatc cttcttctcg
    12481 ttattatcaa gatgaaactg tcgttcgaac agtcagggat tccatggaat ggatgcataa
    12541 acatgtctac aatagtgaaa agagcattgt tgggaactgg tgggattatg aaatcggtac
    12601 acctcgtgcc atcaacaata ccttgtctct gatgaaagaa tacttctctg atgaggaaat
    12661 taaaaaatat acagatgtga ttgaaaaatt tgtaccagat cccgaacatt tccgaaagac
    12721 gactgataac ccatttaagg ctctaggtgg aaacttagtt gatatgggaa gagtaaaagt
    12781 aatagctggt ttactgcgta aggatgatca agaaatttct tctaccattc gctcgattga
    12841 gcaagtgttc aagttggtag accaaggtga aggtttttat caagatggat cctatatcga
    12901 ccacaccaat gttgcctata cgggtgctta tgggaatgtt ttgattgatg gcctgtctca
    12961 actgttgcca gtcattcaaa agaccaagag tccaatcgat aaagataaaa tgcaaaccat
    13021 gtaccactgg attgataaat cgtttgctcc tttgctggtg aatggagagc tgatggatat
    13081 gagtcgtgga cgctcgatca gtcgtgcaaa tagcgagggg cacgtggccg cagtagaagt
    13141 actaagaggg attcaccgaa tagcggatat gtctgaagga gaaaccaaac aacgtttgca
    13201 gagtcttgtg aagaccattg ttcaatcgga tagttattat gatgtcttta agaatttgaa
    13261 gacttataag gatatcagtt tgatgcaatc cttgttaagt gatgcaggag ttgcaagtgt
    13321 tccaagaaca agttacctat ctgcctttaa caagatggat aaaacagcca tgtacaatgc
    13381 agagaaaggg tttggatttg gcttgtcact cttttccagt cgtaccttga attacgaaca
    13441 catgaacaag gaaaataaac gtggttggta tacgagtgat gggatgttct atctttacaa
    13501 tggcgatttg agtcactata gcgatggcta ctggccaaca gttaatccat ataagatgcc
    13561 tggtacaaca gagacggatg ctaagagagc ggatagcgat acaggtaaag ttctaccgtc
    13621 tgctttcgtt ggaacggtca aactagatga tgccaatgcg acagcaacca tggatttcac
    13681 caactggaat caaacattga ctgctcataa gagctggttt atgctgaagg ataagatcgc
    13741 ttttttagga agcaatatcc aaaacacttc aacagatact gctgcaacta caattgacca
    13801 gagaaaactg gaatcaagta atccatataa agtctatgtc aatgataaag aagcctccct
    13861 tacagaacaa gaaaaggatt atcctgaaac ccaaagtgtc tttttagaat cgtccgattc
    13921 gaaaaagaat attggttact ttttctttaa gaagagttca atcagtatga gtaaggcttt
    13981 gcaaaaggga gcctggaagg atatcaatga aggacagtca gacaaggaag ttgaaaatga
    14041 atttcttacg attagtcagg ctcataagca aaatggagat tcttatggct atatgctcat
    14101 tcctaacgtg gatcgtgcca ccttcaatca aatgataaaa gagttagaaa gtagcctcat
    14161 cgaaaataac gaaacccttc agtctgttta tgatgccaaa caaggagttt ggggcattgt
    14221 gaaatatgat gattctgtct ctactatttc caaccaattc caagttttga aacgtggagt
    14281 ctatactatt cgaaaagaag gggatgaata taagattgcc tactataatc ctgaaactca
    14341 ggaatcagct ccagatcagg aagtctttaa aaagctagag caagcagctc agccacaagt
    14401 acagaattca aaagaaaagg aaaaatctga agaggaaaag aaccattcgg atcaaaagaa
    14461 tctccctcag acaggagaag gtcagtcaat cttggcaagt ctagggttct tgctacttgg
    14521 ggcgttttat ttattccgta gaggaaagaa caactaattt gttcatcatc tagatgaatt
    14581 acatgaaact gtggaaaata gaaaaaatcc gaagaaggcc aaatatcttc ggatttttct
    14641 tttaaaaaag gataagttga tacttatcct tttttacttc attattatgt gactatagcg
    14701 ttttgaaact agaataggac gttataacta ctaaaagatt tctataaatt catttgattt
    14761 tcctaatcaa tttgttcgta tcctatttca ctccactata ttagtccgtc tcgctccgtg
    14821 aggtgcgaga caaaacaacc acccgctatg cgggtgcgcg tcgaaggtta taccaaaaaa
    14881 actccaaacg cgatacaata aaggtgttca atccaattgt aaagcgaaag gagaaaaaat
    14941 atggcacaaa aggctcatag tttatctcac acaaagtgga ggtgttctat cacattgtgt
    15001 tcacccctaa gtatagacga aaagtcatct ataatcaata tagaagtagt ttaggcgaaa
    15061 tatttcatcg cttgtgtagt tataaaggtg ttgaaatgat cgagggtcac ttaatgccag
    15121 accatgtaca catgttagtc agtattccgt cgaggctaag tgtttcaagt tttatggggt
    15181 atttaaaagg caaaagtgca ctcatgatgt ttgacaaaca cgccaatctc aagtacaagt
    15241 ttgggaatcg gcatttctgg gcggaaggtt attatgtaag tccagtaggg cttaatgaag
    15301 ccacaattaa gaaatatagt caagattaaa gaaattatcc agtggatgat ttcttcacga
    15361 gtatgaaaat ttgagaacga gtaaagtatg atatatagta aaatgaaata agaacaggac
    15421 aaaatcgatc aggacagtca aatcgatttt taacaatgtt ttagaagtag aggtgtacta
    15481 tttcagtttc aatctactat agcactagat aaattgagtg taaaagaata tgaggatccc
    15541 tttagggata gtggtaagta ataccaaagt ctcttaaaga ggcaagtgac gagtcaagag
    15601 caataaggct tgaacaacgt gaaagccagc gtctttaggc gctggctgat gatttgggct
    15661 tatagctctg agataaacca cccgttagac gggtagttat gattttatct gagtgtaata
    15721 tactgttggg caatctcgct gatgcggtca aagttgcctt gggaagcgag tttattgagt
    15781 tcgccaccaa ttccaacggc atctgcacca gcagcgaacc attgagggat gttgtttagg
    15841 ccgactcctc cggttaccat tacggaaact tgtgggatcg gtgccttgac tgcagagata
    15901 tatgctggac tgagagtact acctgggaag agtttgatga tttcactacc ggcttcaagt
    15961 gcagtcgtga tctctgtgag ggtaatacag cctggaatgt acggtgtgct gtagagattg
    16021 cacattttcg cagtttcagc atggaaagat ggagaaacaa cgtaatttgc tccagctaga
    16081 atggcatctc tagcagttac ggcatcaagc acagtacctg caccgataca aacactctga
    16141 tcgtcctgat acaagtctac aagttccttg atgatttgtc ctgcatactg attggtatag
    16201 gcgatttcaa tagctttgat accgcccttg atacaagcaa tcgaggcttg tagtccttct
    16261 tcctttgtat ttcctcgaat gacagcgaca attttctgtt tttttagttc aataatcgta
    16321 tctgatttgg tcatgtaatt ctcctaacga atgatatctt gtgcatttgc cagtaaattt
    16381 tcaatactag ttgcggaagt ggagagatgg tctcctggaa gagtgcattt gagagttgcg
    16441 ctcgcaactg caaagtcaat ggtagttttt agggaggaat gatggagtag ttggtaaagg
    16501 gcaccagata taaaggcatc cccgctacca attcgttgat agactgcagt ttttagttgg
    16561 acagactctt caaatcgttc ttccagagca taggcttgat agacattttt gtcttgctca
    16621 tcactagatc ggagggtatg gaaaatagcc ttgaaaccat aggcttcttt taaaagtcgc
    16681 atgcgatttt ccacctcttc taggctagca ctgtctcttg gaaacatctc tagattttgg
    16741 tcatcaatca tgagaggatc aataccgaag caatagtcag taaaacgtgc aaacttagaa
    16801 aattcatact tggcttctag gactgaaatc atctttgttc tcagattgag atccattgaa
    16861 acgacaattc ctcggcgctt ggcttcttcc aagagtagga gaaggatcgc acggacctct
    16921 tgaccgatag ctacggtgat tccactaaaa tgaaaatggc taattccctg aaagagagaa
    16981 tccatatcta gcatgtttgg ccgaatctgg ctgatactcg tatgcttacg atcgtagaaa
    17041 acttcacttt gacgacaacc aaagccgttc tccaaatagt agaggccgat tcgatcgcca
    17101 agccgacaga ttgaactggt atcgatttgg tgctgtttca agaatgtgag aaaacgatct
    17161 ccaatctcgt tggcagggag tgcggtaaag actttcgttg agatacccag ggcttgcaaa
    17221 ttacaagcga tgttaatttc tgatccgcca aaataagtcg aactggcaac atgatctccg
    17281 atactggtgg catctaatgg tgagattcga attaatggtt cgccaaacag taatattttc
    17341 gtcatccatt agttcctttc tggtttagaa tagctttact tggctaatac ttctttcaaa
    17401 taagcagcga cttcatcatc ttggcagttt tcaaagaagt attcttggaa atatttgcca
    17461 gagatggtgt ctttgaggaa gtcttggtcg attgttttaa ggatggtcat caaatcattg
    17521 tgggtgattt gtttcacctc gtttaagata cgagcgttgc gttgttcagg gattacacgt
    17581 tctcttgggt agccaccgcc catttcttca gcaaataagc gttcaaacat caatttgagg
    17641 gtcagttctg ccccccagcc aaaccctttg gcataaggga tagacaaggc gttaccacca
    17701 ttgatttgag aataaaggta agcgtcagtt gggtccactg ctagaccaca gacaacacca
    17761 gggaagctgt ttaaagcaag catagcccct acacccgtac cacagccagt aacaacaaag
    17821 tcaactgcct ttgtatttaa aaggatggca gccattagtc cgttctgcac ataagttaat
    17881 tgactttctc cttcttctcc acgcatacca tagttaaata attggtagcc ctttttatct
    17941 gtcgcttctt ttagactatc gtaaataatg tgattcttgc tagcttgact attttcattg
    18001 attaatgcga ttttcataag atcttctttc tatttttact caggttgttt tccgatgtag
    18061 gctaagatac cgccatctac atataggatg tggccattga caaaattgct ggcatcacta
    18121 gcgagaaaga cagcagggcc catcaaatct tcaggatttc cccaacgtgc agcaggtgtt
    18181 tttgcaatga tgaactggtc aaatgggtgg cgagaaccat cttcttgcaa ctcacgaaga
    18241 ggtgctgttt gaggagtggc aatataacct ggtccaattc cgttacattg gatattggct
    18301 ccaccgtatt cagacgcaat gttgcgggtc aacattttca agcccccttt agcagcagca
    18361 taagcgctaa ctgtttcacg tcccagttcg ctcatcatcg aacaaatatt gataatcttt
    18421 ccatgccctt tctttatcat agaaggaata actgcctttg aaacgataaa tggtgcgttt
    18481 aagtcaatat cgatgacctt acggaaatca gcggcgctca tttcgcacat tggaactcgg
    18541 cggataatac cagcgttatt aacgaggatg tcaatgacac caacctcttg ttcgatttgc
    18601 ttgaccatgg cttggatacc gtcctcgtct gtcacgtcac agacatatcc atgggcttgg
    18661 atgccaactt cacgataagc ttcaatccct ttattgacca gatcttgatt gatatcgtta
    18721 aagacaatag tagcgccggc ttcagcgtag gatttggcaa tagcaaatcc aattccataa
    18781 gaagcgccgg tgatgagagc aatttttcct tttaaagaaa actgctcaat tgagaatgat
    18841 gtatttgtca tttgatactt cctttcaaat ttgttaagac ttctctataa ccaaattata
    18901 ttctagtaaa ccggttttgt caataaatat ttttatttat aaaaagattt atttccctta
    18961 aaaatggaat attttgagat ttctgtctat taaaatggtt aaaaagataa tgaaagcgct
    19021 aatatttttt taaaaaaact attgacaaga taaaaataaa ggattacaat ggggaatata
    19081 aagtaaaccg gtaaacctaa acgagaaagg agaaaagatg aaaattgtac ttgtagggca
    19141 tggacatttt gctacaggga tttatagttc tttacaattg attgcaggca atcaagaaaa
    19201 tgtggaggcg attgactttg tggaaggaat gtcagcagat gaactcaagc aaaaaatctt
    19261 acttgcaatt tcaaatgaag aagaagtttt aatcctaagt gatctcttgg gaggatcgcc
    19321 attcaaggtt tcttctacca taatgggaga aaatccagcc aaaacagtga atgttctctc
    19381 gggtttgaac ttagccatgt taatggaagc agtctttgct agaatggctc atagctttga
    19441 tgaggttgtt aataaatcag tagtggcggc ccagggcgga gtcgtaaatg gtaaagaatt
    19501 gttttcaacg gatgcagagg aagaggaaga agatttcgaa tcgggtattt aaagggtaaa
    19561 agaatgataa aaaaggttac gattgaagaa ataaaatcgc ctgagcgctt cttagaagta
    19621 ccacttctga cgaaagaaga agtcggccag gcaatcgata aggttattcg gcagttagaa
    19681 ctcaaccttg actatttcaa ggaagatttc ccgacgccag ctacctttga taatgtctat
    19741 ccaatcatgg ataacacgga atggaccaat ggtttctgga caggagaact gtggttggct
    19801 tatgaataca gtcaacagga tgcatttaaa aacatcgctc ataaaaatgt tctttctttc
    19861 ctggatcgtg tcaataagag agtagaattg gctcaccatg atctcggctt cttgtacaca
    19921 ccgtcttgta tggctgaata taagataaat ggagatggag aggctagaga agcagccttg
    19981 aaagctgcag ataagttgat tgaacgctat caagaaaaag gtggttttat tcaagcttgg
    20041 ggagacttgg gcaagaaaga gcattaccgt ttgattatcg actgcttgct caatatccaa
    20101 ctcttattct ttgcttatca agaaacaggt gatcaaaaat actacgatat tgcagaaagc
    20161 catttctatg cttcagctaa taatgtaatc cgtgatgacg cttcgtcctt ccacaccttc
    20221 tattttgatc ctgagacagg tcaacccttt aaaggtgtaa cgagacaagg gtatagtgat
    20281 gattcatgct gggcacgtgg tcaatcatgg ggagtctatg gtattccttt gacttatcgt
    20341 cacttaaaag acgagtcctg ctttgacttg tttaagggtg tgaccaatta tttcttgaat
    20401 cgtctgccaa aagatcatgt gtcctattgg gatttgattt ttaatgatgg tagtgatcaa
    20461 tcacgagatt cttcagcaac agctatcgcc gtctgtggga ttcatgaaat gctaaaacat
    20521 ctcccagagg tggatgctga caaagatatt tataaacatg ctatgcatgc catgcttcgt
    20581 tccttgatcg aacattatgc aaatgatcaa tttacccctg gtgggacaag tctcctccac
    20641 ggtgtgtact catggcattc aggtaaagga gtggatgaag gcaatatctg gggtgactac
    20701 tattacctag aagcccttat ccgtttctac aaagactgga acctatattg gtaggaggag
    20761 aaatatgaca atgccaaata ttattatgac ccgtatcgat gaacggttga ttcatggaca
    20821 aggacaactt tgggtaaaat acctaggttg taatacggtc attgttgcca atgacgaagt
    20881 aagcacggac aagatgcaac aaactctgat gaaaacagtt gtgccagact cagttgccat
    20941 gcgtttcttc cctttgcaaa aggtgattga tatcattcac aaggctaatc ctgctcaaac
    21001 gatctttatc gttgtaaagg atgtaaagga cgctttaacc ttggtagaag gtggtgtccc
    21061 tatcaaagaa atcaatattg ggaacattca caatgcccct ggtaaagagc aagtgacacg
    21121 ctccatcttc ctgggtgaag aggacaaggc agccctcaag gaattaagcc aaactcatca
    21181 agtaacattt aatacgaaaa caactccaac aggaaatgat ggagctgttc aagtcaacat
    21241 tatggactat atttaaaaga ggagatcgtt atgtcgatta atgtatttca agcgatttta
    21301 attggattat ggacagcttt ctgttttagt ggaatgctgt taggaattta caccaataga
    21361 tgtattgttc tgtcatttgg tgtcggaatt attctaggtg atctgcctac tgctcttgca
    21421 atgggagcta ttggtgaatt ggcttatatg ggattcggtg ttggtgctgg aggtactgtt
    21481 ccaccaaacc caatcggacc tggtatcttt ggtaccttga tggctatcac tagtgctggt
    21541 aaagtcagtc cagaagcggc tcttgccctc tctactccga ttgctgtggc gattcaattc
    21601 ttacaaactt tcgcctacac tgtacgtgct ggtgcgcctg aaacggcgat gaagcacttg
    21661 aaaaaccata atttgaagaa attcaagttc actctaaatg caacaatttg gttgtttgcc
    21721 tttattggat ttaccttggg ttgcttgggt gccctttcaa tggatacctt gttgaaactc
    21781 gtagactaca ttccaccggt attgcttaca ggtttgacag ttgctggtaa aatgctccca
    21841 gctatcggtt ttgcgatgat cttgtcagtg atggctaaga aagagttgat tccctttgtc
    21901 ttgttgggat atgtttgtgc agcttatcta aacatcccaa caattggtat tgcaattgta
    21961 ggtactatct ttgctttgat tgaattttat aacaagccaa aaacagcgga tcatgtggta
    22021 gaggaggaag cacacgatga ctggatctaa caaattaaca aaacgtgatt atcttaaaac
    22081 gtctttgcgg gcattctttt gtcaaaatgg atttaactat agtaactatc aagggttggg
    22141 atatgccaat gtaatgtatc ctgctttgaa aaaacactat ggagaggatc aggaaggttt
    22201 ctaccaagcc ttggaagaaa actgtgaatt ctataatacc aatccacact tcctgccttt
    22261 tattaccagc ttgcaccttg taatgttgga aaatggccgt ccggcaaaag aaacacgtag
    22321 catcaagatg gccttgatgg gaccattggc aggtattggg gattctcttt ctcaattctg
    22381 tttagctcca ttgttctcaa ccatcgcagc ttcgtttgct caagaaggct tggttgtcgg
    22441 tccaatcttg ttcttccttg cgatgaatac gattttaaca gcgattaaat tgtcaactgg
    22501 tctgtatgga tacaaactag gaacaactgt gattgataaa ctaagcgaac agatggcaac
    22561 gatttctcgt attgccaata ttatcggtgt aaccgtaatt gctggtttgg cagcgacatc
    22621 tgttaagatt atggtgccga ttacctttgc tgcaggggaa gttaaagcag acgctaaaca
    22681 aagtatcgta agtattcagg gaatgcttga taaggttgct ccagctcttc taccagccct
    22741 atttacactt ttagtttact acttgatcaa agaaaagaaa tggacaacat ataaactcgt
    22801 tattttaaca gttatcatcg gaattatcgg aagctggctt aagattatag cttaatggat
    22861 acaacattgt tttatggaat agtgattgtc ttggcagtga gtcccctttt actgtcaagc
    22921 tttcattcta ttcgtcagca aaagttgctt cgcaaacaga tggagcaacg acaagagtat
    22981 ttagcttctt taacatctgg tgatgaagtg ttgttattgt ctggaattca tggaaaaatc
    23041 atttctatca aagatgactt ggtctccttg cagattgcaa aaggtgtggt catctatgta
    23101 gaaaaggaaa gtgtaatggg aaagacaaaa gaactgcttt ttaagtagtt ctttttctgg
    23161 taatagataa tgaaagaaga aagaagacaa ttttttgaaa gagtcgatgg aaaccaatgt
    23221 cgtgattata tcttgtccca ctgttcaaaa gactatgaga aagtcaagag ttcccttgaa
    23281 cgcttgatgg acaatcgttt tatgtttgat agtccttggg atatggagcc ttgttcaaaa
    23341 atccatcaaa tccagccgat ggtatgggat caagtatttg aagatgatcc agaatggtct
    23401 tatatgctca atcgacaaga atatctcttg cagtttatga tagggtatct ggtagaagga
    23461 gataaggact atattcaaaa gtgcaagttc tttctatttg attggattga gcaggtgaga
    23521 gaattttctc ctcaatcctt gatgactaga accttggata cgggtattcg ttcctttact
    23581 tggttgaaac tactcttgct cctcttgaaa tttgacttgc tagaggagaa agaactagag
    23641 aaaattttgg tcagtctaga aaagcagatt gactttatga aaagctacta tcgcgccaag
    23701 tacaccctta gtaactgggg gattttacaa acaattccga tgcttgctat ctatcatttc
    23761 ttttcagata agatggacct agaagaagct taccattttg cttcagagga gttgaaacag
    23821 caaattgaga cacagatttt aggagatgga agccagtttg aacagtcgat tctctatcat
    23881 gtagaggttt ataaagcctt gctggatttg tgtctcttgc ttccagactt gcaagatagt
    23941 taccaagagt tgctggaaaa gatggcgacc tatattcaaa tgatgacagg cttagatgga
    24001 cggactttgg cttttggtga tagcgattct acagaaacga cagaaatgtt gagcctgtct
    24061 gctgtggttt tgaacaagga agaccttctt aacggtctgg atgttaaagt cgatttgctt
    24121 agcctcttgt tcctggggcg agaaaaggtc aagcgactgc aggaatttga aaggagagct
    24181 tggcagccta agtccatgat ctttgaagac tctggacatg tctgcattaa ggatgaacat
    24241 cgttatctat ttttcaaaaa tggtccgcta ggaagtgccc atagccatag tgacgagaat
    24301 agtttttgct tacagtatca aggccaacct attttcatag atgctgggcg ttattcttat
    24361 cgggagatat atgaacgtta tctcttaaag agtgcttgga gtcattcgac ctgcattgta
    24421 gatgggaaag ctccggaaag aatcacggga tcctgggaat atgaatacta tcctcactcc
    24481 ctgttttgtc accataaaga aagggaggga gtgcattata ttgagggggc ttattggtca
    24541 gcagaacctg atttgcctta tcttcacaag agaaaaatcc tcatgttggt agaggatgtc
    24601 tggctcttgg tagatgacat caggtgtcaa ggtcagcatg aggcgttgat tcagtttatc
    24661 cttgacaagg atgtgaccta tcaagatggg aaaatcaatc agttgagact atggagtgaa
    24721 gttgattttg atttggaaga taccatcatt tctcctaaat acaatgagct tgaaagaagt
    24781 agcaagctca ccaagcgcca attctttgag aatcagatgc tggattatac catcattgcg
    24841 catgagagtt ttgaaatcat ccgtcattct gtctaccaga cagatgatcg tgaagtggaa
    24901 aatgctctgg cttttgaagt gaaaaatgac gaaacagaca agctgattct gttattaagc
    24961 gaggatattg gtgtaggtga aaaattgtgc ctcgttgacg gaacaaaaat gcgtggaaaa
    25021 tgtctagtat atgataaaat aaatgagaga atgattcgct tgcagtgcta gaaataggca
    25081 ttttgaatag tgaatatgtt ataataagta ttagtaggag gtgttttaga ttggagaaga
    25141 aactgaccat aaaagacatt gcggaaatgg ctcagacctc gaaaacaacc gtgtcatttt
    25201 acctaaacgg gaaatatgaa aaaatgtccc aagagacacg tgaaaagatt gaaaaagtta
    25261 ttcatgaaac aaattacaaa ccgagcattg ttgcgcgtag cttaaactcc aaacgaacaa
    25321 aattaatcgg tgttttgatc ggtgatatta ccaacagttt ctcaaaccaa attgttaagg
    25381 gaattgagga tatcgccagc cagaatggct accaggtaat gataggaaat agtaattaca
    25441 gccaagagag tgaggaccgg tatattgaaa gcatgcttct cttgggagta gacggcttta
    25501 ttattcagcc gacctctaat ttccgaaaat attctcgtat catcgatgag aaaaagaaga
    25561 aaatggtctt ttttgatagt cagctctatg aacaccggac tagctgggtt aaaaccaata
    25621 actatgatgc cgtttatgac atgacccagt cctgtatcga aaaaggttat gagcattttc
    25681 tcttgattac agcggatacg agtcgtttga gtactcggat tgagcgggca agtggttttg
    25741 tggatgcttt aacagatgct aatatgcgtc acgccagtct aaccattgaa gataagcata
    25801 cgaatttgga acaaattaag gaatttttac aaaaagaaat cgatcccgat gaaaaaactc
    25861 tggtatttat ccctaactgt tgggccctac ctctagtctt taccgttatc aaagagttga
    25921 attataactt gccacaagtt gggttgattg gttttgacaa tacggagtgg acttgctttt
    25981 cttctccaag tgtttcgacg ctggttcagc cctcctttga ggaaggacaa caggctacaa
    26041 agattttgat tgaccagatt gaaggccgca atcaagaaga aaggcaacaa gtcttggatt
    26101 gtagtgtgaa ttggaaagag tcgactttct aaaatgaagg aaaatgactt gcaatctctg
    26161 ttaagaaata aaataatccc acctagaaca agctaggtgg gattatttgc ctatgaaatg
    26221 agaaattatg ggagcaagct cctaaatcaa ctgtttttga tctacttctt taactacttg
    26281 ataaaagtta tagaagtagg ccaaacttga aatgatggtt acgactagga atattgaaaa
    26341 tttccattgg acagggttgc ttaaaagttg tggaaaggat atgaggagaa agaagagggc
    26401 tgcgttgagg acaggtatcc gttttgattg tattttctca agtcctttat tgagcgcagg
    26461 aagaaagagg agtaggagta gtaaaactgt atgagaaaca gctcctgaaa taagggcgaa
    26521 gaaaaggaaa atactgataa aaacatgaat gatcagtagt ctagctagtg atttcataag
    26581 gcacctccta atcctggtct tttttagctc ttgcaatacg aagtgagtcg acaatatgta
    26641 tcatcactcc gaaaaagaaa gctcccagta tagttttaaa aatatgtttt gtatttagaa
    26701 gagaactgat aaaatttgga ttttcacttg ttagggtatc aatgagtgga attataaaaa
    26761 atatcactgt tccataaatt gaacctgctt tcagaccagg ataacgtaac tgtttctttt
    26821 cttttttcat gagtttcctc ctaatcctca tcttgatttt tcttagtttt tgcaatgcga
    26881 cgggagatga ggaactgtat gctcgctccg aagaaaatag aaccgagaat gcttgataca
    26941 ccatttctta tagtgagaag agaatgaaaa tagtcctgac cttcatctat gagtatactg
    27001 agaagaggag ttataaaaaa catccataga ccaaagaaca aacctgcttt aagacctagg
    27061 tagtgtagtt gcttgctttc tttctcattc agcatatctg gttcaatgac tgtgatgcct
    27121 gtttttttca tttggtaggt gacatagcca gcagcgatga gggcaatcac taaaatcaga
    27181 ggaggataga ttagagccac ttcttgaggg tatttatagg ccagaaggag tggaataaga
    27241 tttccgaaaa tcatcagata aaagaggatg ataaagactt ggttcccaat actatcggcc
    27301 tcacgccgtt tgtattcgtc aaggggacca gaaataccgt atgtgcgttt gatcagtttt
    27361 tcagtgaagg tttctttttt catgagtttg ctcctttttt aaaaatcttc ctcccaaaag
    27421 agactgttga ggtcagtttg gaggctgcgg gcgagattga gacagagttc caaggttgga
    27481 ttgtacttgt cgttttcaat catattgata gtctgtctcg agacaccgat atccttggcg
    27541 agttcgagct gggaaatacc caattccttg cgaaattctt tcacacgatt catctgatct
    27601 cctttttgat ttatgtcgta tatatttgac tttattatag tcttttaaac ataaagtgtc
    27661 aagtattttt gacatatttt ttgaagaaat agtagtctcc ttgtcctatt tgtctgacaa
    27721 gtgcaagctg gtcggatttg tggtaaaata gataagatat gacaaaagaa tttcatcatg
    27781 taacggtctt actccacgaa acgattgata tgcttgacgt aaaacctgac ggtatctacg
    27841 ttgatgcgac tttgggcgga gcaggacata gcgagtattt attaagtaaa ttaagtgaaa
    27901 aaggccatct ctatgccttt gaccaggatc agaatgccat tgacaatgcg caaaaacgct
    27961 tggcacctta cattgagaag ggaatggtga cctttatcaa ggataacttc cgtcatttac
    28021 aggcacgttt gcgcgaagct ggtgttcagg aaattgatgg aatttgttat gacttgggag
    28081 tgtctagtcc tcaattggac cagcgtgagc gtggtttttc ttataaaaag gatgcgccac
    28141 tggacatgcg gatgaatcag gatgctagtc tgacagccta tgaagtggtt aatcactatg
    28201 actatcatga tttggttcgt attttcttca aatacggtga ggataaattc tctaaacaga
    28261 ttgcgcgtaa gattgagcaa gcgcgtgaag tgaagccgat tgagacaacg actgagttag
    28321 cagagattat caagttggtc aaacctgcca aggaactcaa gaagaagggt catcctgcta
    28381 agcagatttt ccaggctatt cgaattgaag tcaatgatga actgggggcg gcagatgagt
    28441 ccatccagca ggctatggat atgttggctc tggatggtag aatttcagtg attacctttc
    28501 attccttaga agaccgcttg accaagcaat tgttcaagga agcttcaaca gttgaagttc
    28561 caaaaggctt gcctttcatc ccagatgatc tcaagcccaa gatggaattg gtgtcccgta
    28621 agccaatctt gccaagtgcg gaagagttag aagccaataa ccgctcgcac tcagccaagt
    28681 tgcgcgtggc cagaaaaatt cacaagtaag agggaaaaag atggcagaaa aaatggaaaa
    28741 aacaggtcaa atactacaga tgcaacttaa acggttttcg cgtgtggaaa aagcttttta
    28801 cttttccatt gctgtaacca ctcttattgt agccattagt attattttta tgcagaccaa
    28861 gctcttgcaa gtgcagaatg atttgacaaa aatcaatgcg cagatagagg aaaagaagac
    28921 cgaattggac gatgccaagc aagaggtcaa tgaactatta cgtgcagaac gtttgaaaga
    28981 aattgccaat tcacacgatt tgcaattaaa caatgaaaat attagaatag cggagtaaga
    29041 tatgaagtgg acaaaaagag taatccgtta tgcgaccaaa aatcggaaat cgccggctga
    29101 aaacagacgc agagttggaa aaagtctgag tttattatct gtctttgttt ttgccatttt
    29161 tttagtcaat tttgcggtca ttattgggac aggcactcgc tttggaacag atttagcgaa
    29221 ggaagctaag aaggttcatc aaaccacccg tacagttcct gccaaacgtg ggactattta
    29281 tgaccgaaat ggagtcccga ttgctgagga tgcaacctcc tataatgtct atgcggtcat
    29341 tgatgagaac tataagtcag caacgggtaa gattctttac gtagaaaaaa cacaatttaa
    29401 caaggttgca gaggtctttc ataagtatct ggacatggaa gaatcctatg taagagagca
    29461 actctcgcaa cctaatctca agcaagtttc ctttggagca aagggaaatg ggattaccta
    29521 tgccaatatg atgtctatca aaaaagaatt ggaagctgca gaggtcaagg ggattgattt
    29581 tacaaccagt cccaatcgta gttacccaaa cggacaattt gcttctagtt ttatcggtct
    29641 agctcagctc catgaaaatg aagatggaag caagagcttg ctgggaacct ctggaatgga
    29701 gagttccttg aacagtattc ttgcagggac agacggcatt attacctatg aaaaggatcg
    29761 tctgggtaat attgtacccg gaacagaaca agtttcccaa cgaacgatgg acggtaagga
    29821 tgtttataca accatttcca gccccctcca gtcctttatg gaaacccaga tggatgcttt
    29881 tcaagagaag gtaaaaggaa agtacatgac agcgactttg gtcagtgcta aaacagggga
    29941 aattctggca acaacgcaac gaccgacctt tgatgcagat acaaaagaag gcattacaga
    30001 ggactttgtt tggcgtgata tcctttacca aagtaactat gagccaggtt ccactatgaa
    30061 agtgatgatg ttggctgctg ctattgataa taataccttt ccaggaggag aagtctttaa
    30121 tagtagtgag ttaaaaattg cagatgccac gattcgagat tgggacgtta atgaaggatt
    30181 gactggtggc agaatgatga ctttttctca aggttttgca cactcaagta acgttgggat
    30241 gactctcctt gagcaaaaga tgggagatgc tacctggctt gattatctta atcgttttaa
    30301 atttggagtt ccgacccgtt tcggtttgac ggatgagtat gctggtcagc ttcctgcgga
    30361 taatattgtc aacattgcgc aaagctcatt tggacaaggg atttcagtga cccagacaca
    30421 aatgattcgt gcctttacag ctattgctaa tgacggtgtc atgctggagc ctaaatttat
    30481 tagtgccatt tatgatccaa atgatcaaac tgctcggaaa tctcaaaaag aaattgtggg
    30541 aaatcctgtt tctaaagatg cagctagtct aactcggact aacatggttt tggtagggac
    30601 ggatccggtt tatggaacca tgtataacca cagcacaggc aagccaactg taactgttcc
    30661 tgggcaaaat gtagccctca agtctggtac ggctcagatt gctgacgaga aaaatggtgg
    30721 ttatctagtc ggggtaaccg actatatttt ctcggctgta tcgatgagtc cggctgaaaa
    30781 tcctgatttt atcttgtatg tgacggtcca acaacctgaa cattattcag gtattcagtt
    30841 gggagaattt gccaatccta tcttggagcg ggcttcagct atgaaagact ctctcaatct
    30901 tcaaacaaca gctaaagctt tggagcaagt aagtcaacaa agtccttatc ctatgcctag
    30961 tgtcaaggat atttcacctg gtgatttagc agaagaattg cgtcgcaatc ttgtacaacc
    31021 catcgttgtg ggaacaggaa cgaagattaa aaacagttct gctgaagaag ggaagaatct
    31081 tgccccgaac cagcaagtcc ttatcttatc tgataaagca gaggaagttc cagatatgta
    31141 tggttggaca aaggagactg ctgagacctt tgctaagtgg ctcaatatag aacttgaatt
    31201 tcaaggttcg ggctctactg tgcagaagca agatgttcgt gctaacacag ctatcaagga
    31261 cattaaaaaa attacattaa ctttaggaga ctaatatgtt tatttccatc agtgctggaa
    31321 ttgtgacatt tttactaact ttagtaggaa ttccggcctt tatccaattt tatagaaagg
    31381 cgcaaattac aggccagcag atgcatgaag atgtcaaaca gcatcaggca aaagctggga
    31441 ctcctacaat gggaggtttg gttttcttga ttacttctgt tttggttgct ttctttttcg
    31501 ccctatttag tagccaattc agcaataatg tgggaatgat tttgttcata ttagtcttgt
    31561 atggcttggt cggattttta gatgactttc tcaaggtctt tcgtaaaatc aatgaggggc
    31621 ttaatcctaa gcaaaaatta gctcttcagc ttctaggtgg aattatcttc tatcttttct
    31681 atgagcgtgg gggcgatatc ctgtctgtct ttggttatcc agttcatttg ggatttttct
    31741 atattttctt cgctcttttc tggctagtcg gtttttcaaa cgcagtaaac ttgacagacg
    31801 gtgttgacgg tttagctagt atttccgttg tgattagttt gtctgcctat ggagttattg
    31861 cctatgtgca aggtcagatg gatattcttc tagtgattct tgccatgatt ggtggtttgc
    31921 tcggtttctt catctttaac cataagcctg ccaaggtctt tatgggtgat gtgggaagtt
    31981 tggccctagg tgggatgctg gcagctatct ctatggctct ccaccaagaa tggactctct
    32041 tgattatcgg aattgtatat gtttttgaaa caacttctgt tatgatgcaa gtcagttatt
    32101 tcaaactgac aggtggtaaa cgtattttcc gtatgacgcc tgtacatcac cattttgagc
    32161 ttgggggatt gtctggtaaa ggaaatcctt ggagcgagtg gaaggttgac ttcttctttt
    32221 ggggagtggg acttctagca agtctcctga ccctagcaat tttgtattta atgtaagaat
    32281 ggcatcctga tgtttcaggg tgtttttgta tttaaataca caatgaaaat caaagaacaa
    32341 actagaaagc taactttagg ctgctcaaaa tatagtagat tgaaactaga atagtacaca
    32401 tctacttcta aaa
//
LOCUS       DAVBDG010000020        31591 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBDG010000020 DAVBDG010000000
VERSION     DAVBDG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.020"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(210..2225)
                     /gene="recG"
                     /locus_tag="VQ526_000900"
     CDS             complement(210..2225)
                     /gene="recG"
                     /locus_tag="VQ526_000900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2554640.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(2244..3347)
                     /locus_tag="VQ526_000901"
     CDS             complement(2244..3347)
                     /locus_tag="VQ526_000901"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2554641.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(3337..3705)
                     /locus_tag="VQ526_000902"
     CDS             complement(3337..3705)
                     /locus_tag="VQ526_000902"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2554642.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(3744..4775)
                     /locus_tag="VQ526_000903"
     CDS             complement(3744..4775)
                     /locus_tag="VQ526_000903"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2554643.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(4777..5808)
                     /locus_tag="VQ526_000904"
     CDS             complement(4777..5808)
                     /locus_tag="VQ526_000904"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2554644.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(5889..8402)
                     /gene="secA"
                     /locus_tag="VQ526_000905"
     CDS             complement(5889..8402)
                     /gene="secA"
                     /locus_tag="VQ526_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2554645.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQSMIKRTLERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(8548..8836)
                     /locus_tag="VQ526_000906"
                     /pseudo
     CDS             complement(8548..8836)
                     /locus_tag="VQ526_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8864..9496)
                     /locus_tag="VQ526_000907"
     CDS             complement(8864..9496)
                     /locus_tag="VQ526_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2554646.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(9500..10141)
                     /locus_tag="VQ526_000908"
     CDS             complement(9500..10141)
                     /locus_tag="VQ526_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554647.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            complement(10134..10925)
                     /locus_tag="VQ526_000909"
     CDS             complement(10134..10925)
                     /locus_tag="VQ526_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554648.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(11110..11445)
                     /locus_tag="VQ526_000910"
     CDS             complement(11110..11445)
                     /locus_tag="VQ526_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554649.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(11585..11920)
                     /locus_tag="VQ526_000911"
     CDS             complement(11585..11920)
                     /locus_tag="VQ526_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554650.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWIYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(12011..13321)
                     /gene="der"
                     /locus_tag="VQ526_000912"
     CDS             complement(12011..13321)
                     /gene="der"
                     /locus_tag="VQ526_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2554651.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(13335..14048)
                     /locus_tag="VQ526_000913"
     CDS             complement(13335..14048)
                     /locus_tag="VQ526_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2554652.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDVKPRLPLENVVFEEEYQEQSTETIQAYDRVQADYAGGRATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(14045..14941)
                     /gene="dnaI"
                     /locus_tag="VQ526_000914"
     CDS             complement(14045..14941)
                     /gene="dnaI"
                     /locus_tag="VQ526_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2554653.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(14942..16111)
                     /locus_tag="VQ526_000915"
     CDS             complement(14942..16111)
                     /locus_tag="VQ526_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2554654.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(16112..16585)
                     /gene="nrdR"
                     /locus_tag="VQ526_000916"
     CDS             complement(16112..16585)
                     /gene="nrdR"
                     /locus_tag="VQ526_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2554655.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            16725..17090
                     /locus_tag="VQ526_000917"
     CDS             16725..17090
                     /locus_tag="VQ526_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2554656.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            17095..17790
                     /locus_tag="VQ526_000918"
     CDS             17095..17790
                     /locus_tag="VQ526_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554657.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            17807..18574
                     /locus_tag="VQ526_000919"
     CDS             17807..18574
                     /locus_tag="VQ526_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554658.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(18927..20126)
                     /locus_tag="VQ526_000920"
     CDS             complement(18927..20126)
                     /locus_tag="VQ526_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554659.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(20123..21016)
                     /locus_tag="VQ526_000921"
     CDS             complement(20123..21016)
                     /locus_tag="VQ526_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554660.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQEIYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            21723..21911
                     /locus_tag="VQ526_000922"
                     /pseudo
     CDS             21723..21911
                     /locus_tag="VQ526_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            22326..22943
                     /locus_tag="VQ526_000923"
     CDS             22326..22943
                     /locus_tag="VQ526_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2554661.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(22986..23873)
                     /locus_tag="VQ526_000924"
     CDS             complement(22986..23873)
                     /locus_tag="VQ526_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2554662.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(24022..25905)
                     /locus_tag="VQ526_000925"
     CDS             complement(24022..25905)
                     /locus_tag="VQ526_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2554663.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            26281..27735
                     /locus_tag="VQ526_000926"
     CDS             26281..27735
                     /locus_tag="VQ526_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2554664.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLIFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDCFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            27716..28681
                     /locus_tag="VQ526_000927"
     CDS             27716..28681
                     /locus_tag="VQ526_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2554665.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     SYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILIIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(28716..29990)
                     /locus_tag="VQ526_000928"
     CDS             complement(28716..29990)
                     /locus_tag="VQ526_000928"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2554666.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(29990..31162)
                     /locus_tag="VQ526_000929"
     CDS             complement(29990..31162)
                     /locus_tag="VQ526_000929"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2554667.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTVLSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(31293..31541)
                     /locus_tag="VQ526_000930"
     CDS             complement(31293..31541)
                     /locus_tag="VQ526_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554668.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
BASE COUNT         9248 a         7098 c         5967 g         9278 t
ORIGIN      
        1 aggatgatag gctgaaagta gttttagaaa cgaaaaagag catttcgctc tctcttctgt
       61 ttatactagt tttttgcttc cttcttttgg aaagtggttt ggtaatttac tttaatcgtt
      121 actgtcatat gagagtcctc gtttcttttt gatgacttta gtataagagc caaacctaaa
      181 aactagctta agttttcctt agagaaagct taatccagat gttctttctt ttccagatga
      241 agggcaatca tgcgccactc tggatcttct tgccaagctt ctatagaact aatgtagcta
      301 gcaacctttc ttgcttcttc taaaatcgga aaatcttcga taatatcagc cacttggaac
      361 tctggaagtc ctgactgtct ggttccaaaa atctcaccag aaccacgcat tttcaaatct
      421 tcctccgcaa ggacaaatcc attggtcgtt tctgtcatga tacgcatgcg gtctttccca
      481 gaatccgtct tgggattagc aacgagaaca gcgtaggact gcttgtcccc ccgaccgaca
      541 cgacctctaa gctggtgaag ttgactgaga ccgaagcgat cggcatccat gataatcatg
      601 acagtcgcat tgggaacgtt gaccccaacc tcaataaccg tcgtcgaaac cagaatatcc
      661 gtctttctct ccttgaaatc ctgcatgatc tggtcttttt cgtcactctt catcctacca
      721 tgtagaagag ccacctctgc cttgcctgca aaatgagtcg tcaactcctc tgataaggca
      781 atggcatttt tcaaatctag agcttctgat tcttcaatca aaggagagat gacatagact
      841 tgggaacctt tttgaatttc cccctctaac caagtcaaga cctgaggtag ttgctcatgt
      901 ttgatccagc gcgtcacaat aggcttccga cctgctggca tctggtcgat aatggaaaca
      961 tccatatctc caaaggctgt gatggcaagc gtccgtggaa tgggagtcgc cgtcatcatg
     1021 aggacatctg gattgtcacc tttttcccgt aaaatacgcc tttgccctac accaaaacgg
     1081 tgctgctcat cgataataat caaaccaaga cgagcatact ccaccccatc ttgtatcaga
     1141 gcgtgagttc ctataatcaa atcagcctca cccttggcaa tggtctccaa gacttctctc
     1201 ttttctgcag ctttcaagga acctgtcaag agagccagtt tcaaattggg aaaaaggttc
     1261 tgtaaactct caaagtgttg ctctgcgagg atttctgttg gtaccattag ggcagcctga
     1321 taacctgctg tcactgccgc aaacatggcc aagccagcga ctaccgtttt tccactcccc
     1381 acatcccctt gtaggagacg attcatgtgg tggtcggact tcatatcagt taaaatttcc
     1441 tgcaaactct tttcctgagc ttgggtcagg gcaaaaggaa gacttacttt aactgctgtc
     1501 actttttcct gagaccaatt cagaaccaga ccacttccct gaactctatt ttcagacttg
     1561 agcatctgca gctgcatttg gaaataaaag agttcctcaa actttatacg gcgaagagcc
     1621 tgcttgtatt ctgccaaatc ctttggaaaa tgcatagcac ggactgcctg acaacgggac
     1681 atgagtttgt atttgtctag taaagactgg ggcagatttt cttctatcaa gaggtccagt
     1741 ccctgatcaa aagccgtctt gatgaccttg accagactgg cctgactgat tccctgagcc
     1801 agacgataga caggctggag gtcatcttct acctgagcca gaaccttcat cccagtcaga
     1861 ctagccttag cgcggtccca ttttccaaag acagcaaggg ttgctcccaa ctctatttta
     1921 tcagccagat agggctggtt aaagaaattc accgcaaaaa cgacctctcc ctgcttgaga
     1981 ctaaaacgca ggcgattgcg cttgaaacca taatactgga cactagcagg agtcactacc
     2041 tgaccagaaa gaactgcctt ctcaccgtct tccagctcca gcacctgctt ggttttgaag
     2101 tcttcataac ggaaaggaaa gtagagcaag agatcttgca agttttcaat tcctagtttg
     2161 gcgtattttt ctgctgactt tggtcccaca ccaggcaaga catgcaaggg ttgatgtaga
     2221 ttcatgctcc actcctttct tttctaataa tattctctcg gaatacggtc gctgaggagg
     2281 caaaccacct catagttaat ggttacgcgg taggtcgcta cctgagttgc agtgatttcc
     2341 ttatccccat tggagccaat caaggttacc ttggttccta gcggataaag cttaggcaat
     2401 cgaatagtga tttggtccat cgaaaccctg ccgacaattg ggcaagcttg gccatctacc
     2461 aagacagaga aattttgcat gtctcttgtc catccatctg catacccgat tggcacggtc
     2521 gcgatgactt gctcgctatc cgcttgataa gttgctccat agcccatgca agctccagct
     2581 ggaactgtct tgacatgaac cagagcagac tccaaggtca aggccggtat caaatcataa
     2641 ggcaaatcca agaccgctcc acttggattg aggccataca tggcatctcc catacgaacc
     2701 gcattgaaaa tagtctctac atgccaaaga gtcgttgcag aattgctagc atgaaccagc
     2761 tctggaactt ccttcatact agctaaaata gttttaaacc gttctaactg ggcattaaaa
     2821 tagtcatctg attcctcatc agcagtagca aagtgggtaa agattccttc aacacaaaca
     2881 ccgtgttgtt ggagcaaatc ttgagcctgc tcaacctcac ttgcctctct aaaaccaatc
     2941 cgtcccatcc ctgaatcaat cttgaggtgg actgtcaatc cagttaggtc cacttcctta
     3001 tctaagagtg cttgaatcca ctccagtcca gccactgtca aggtgaagtc atattcttta
     3061 gctagagcaa cagcttcgat ttcagaaact cctaaaatga ggattggctt gctgagtcca
     3121 gcttgtctga gttcaatggc ttcatcgata ttggaaacgc aaaagccatc aacatcatct
     3181 tgaattgcct tggcaacggc aacagctcca tgaccataag cattggcctt aaccacagcc
     3241 aacttgagcg ttccttgagg gatatgagcc cccatttgct gaatattttg tcgaatagct
     3301 cccagatgaa tcagagcctt ggttggtcta tgtggactag ctttcatgat tttcctccaa
     3361 aatgacactg gctgtcacaa actgatcggt gtggctgata gacagccaaa tctttcctga
     3421 aaatggtgcc tgactaaaat aaggcgcccc acgttcattg ttcaagactt ccaaatcctg
     3481 aaaaccgagc ttgctaatgc ccgttcccat agccttggaa aaggcctcct tagccgacca
     3541 gcgaccagct aaatattcta tttgcctgcg tcctttgaga ctggtgaagc gctccatttc
     3601 ctgagcggtc agtacacgct tagcaaatcc ttcatgtcgt gtaactgcgc tttctatcga
     3661 agccaattct tcgatgtcaa ttccgtgtcc aactatcatt ctcatcaaaa ggagttgaga
     3721 ttccccaact ccatcctttt cacttatttt gtcaaggtaa cataaatctc ttctaccaag
     3781 gcctctgtat tttcccaacc taggcaaggg tcagtaatag agcaaccaaa gatctctggt
     3841 tggttttgac gaccatctgc taggtaagat tcaatcataa atcctcgaac cgtcttttta
     3901 attttctcat tccaatcacg attctgcaag gtctggcgaa caattcgaat ctgctccata
     3961 tattgcttgc ctgagttatc atggttggtg tcaatgagga taaaaggatt ttcaagtccc
     4021 atggtttcat agcgttcaat ggcttggagt agattttcat agtagtaatt cggcatataa
     4081 ttgccatact cgttgactgc tccacggagg ataacatggg ccaaaggatt acctgatgtc
     4141 tcaacttcct gcccatgata aaggaaggtt tgcttgtttt gagcagcata gatggcgtta
     4201 aacataacac ccaaatttcc tgaggttgga tttttcatcc ctactggtgc atcaatccca
     4261 gaagccacaa agcggtgctc ttggtcttcc acagaacgag ctccaacggc atggtagctg
     4321 accaagtcat ccaccaagat cagatttgac ggataaagca tctcatctgc cgttgtcaaa
     4381 ccagtctctg taatcacgcg gtagtgcaac tggcgcacag cctgcaagcc attaatcagg
     4441 cttggagcct tagaagtatc tggctggtga actaatcctt tatagccgtc tccattggta
     4501 cgaggcttag cagtatacac gcgcatgacc atgaaaatct tatccgctac cttcttttgc
     4561 aaggcagata aacggcgagc atattccaag accgcctctt cattatcaga agagcaagga
     4621 ccaatcacca agagaatacg gtcatcttcc cctgaaataa tatctgccaa ttccctgtca
     4681 cggctttcct tgagtctcaa ggcttccgca gacaattggg tttcagcctt gatgacttcc
     4741 atatcgattt cttgaccttt ttcaataaat gccatcttat tctcctaacg ttttgtaaat
     4801 ttctctgaca agagcttctg tgttatccca acccaggcaa gggtctgtga tagacttacc
     4861 aaatacttct ggctcatttt gtcgaccatc ttccagataa gactcaatca taaaaccacg
     4921 aacgaactgc ttaatttttt cattccaagc acggttaatc aaggtctggc ggacaattcg
     4981 gatctgttca atatactgct taccagaatt gtcatgattg gtatcaatga tgataaaagg
     5041 attttccaag cccattttct catactgggc aatggtatca attaaattgt cataatagta
     5101 gttgggaata ttttttccat actcattaag agcaccacga agaatagcgt gtgaaagcgg
     5161 gttcccagtt gtttctactt cttttcctaa gaaaaggaaa ctttgtttgt tttgagcagc
     5221 ataaatccca ttaaacatga cattgagatt tccagaggtt ggatttttaa aaccagtaga
     5281 aaatcctgcc ccacttgcca caaagcggtg ttgctggtct tcaactgaac gggcaccaac
     5341 tgccatgtaa gaaatcaaat catctacaag cggaaggttt tcaggataaa gcatttcatc
     5401 agcagttgtc atccctgttt ctgtgatgac acgatagtga agatggcgaa cggctttgat
     5461 tccattgata agactaggcg cttctgtcgc gttaggctgg tgaatcaagc ccttatagcc
     5521 atctccgttg gtacggggtt tggcagtata aacacgcata accataaaga tacgatctgc
     5581 cacttcttct tgtaggactg ccaaacgctt agcgtattca aggacagctt cttcgttgtc
     5641 agatgagcat ggcccgatta ccaagagaat tcgctggtct tctccacgta taatggcttc
     5701 tagctcttga tctcgctgtg atttcctctc caaagcctga ccttctaatt ttgacaaggc
     5761 acgaacttct tctatattta ttttagagct ttttgctgta aataccataa ctcatctcct
     5821 tataaagcaa acggataccc agtaaaaatt tacttttcac aagatatccg cctctaaact
     5881 atctcatttt attgtctttt accgtgacag tttttaaatt tcttaccaga accacatggg
     5941 caaagttcat tgcgtccaat ctggctcaaa tccaaatctt ctggcatact tgcttggtga
     6001 gcagcgatat tgcgagtcgc tgttgtactg atatgacgtt ctgcctgtgg tctttcttgt
     6061 tcatgaattt gtgctttcat catcaagcgt gtcacatcaa actcaatcga accaatcata
     6121 tcattaaaca tacggaaacc ttctgcctga tactcaacaa cagggttgtt ctgagcatag
     6181 ccacgaagtc caaccgcgtt acgcaattga tcaagggcat cgatatgatc tgtccacttg
     6241 ttatccacca ctcgtagaat caaaactttt tggaattctt taactgcttc ttcatcgcgt
     6301 agttttgaaa cctgactatc gtaaaccttc aaggcacgtt ggaaaagctc ttccttgatg
     6361 gccttatcag acaagcctga caagtcttcc atcgtaatag aatcttcagg aagcaagttg
     6421 tacttagcaa agttcaaaat tgcctctagt ttttcatctt gtttggcacg cgcatgacca
     6481 tcaacgacac gttcaagcgt gcgcttaatc atagactgaa tttcaggtgc caagtcacgg
     6541 tctgcagtaa tgacatcata acgttgagta tagataatct cacgttgttc acgcatgaca
     6601 tcgtcgtatt gaaggacttg tttacgggta tcgtagttat ttccttcgac acgtttctga
     6661 gctgcttcaa cctgacgcgt caacatgcga gactcaatcg cctcttcaga catgttcaag
     6721 cgttcaaaga ttcccttcaa gcgttcagaa ccaaaacgtt tcatcaaatc atcttcaaga
     6781 gataggtaga attgtgactc acctggatct ccttgacgac ctgaacgtcc acgaagctgg
     6841 ttatcgatac gacgactttc atgacgttct gtaccaataa cacaaagtcc tccaagttca
     6901 cgaacacctt caccaagctt gatgtcggta ccacgacccg ccatgttggt tgcgatggta
     6961 acggcaccac gttgaccagc attcatgatg atttgggctt ctctatagtg gtttttggca
     7021 ttcaagactt cgtgaggaac accagctgca accaatttct tagaaatgta gtcactagtt
     7081 tcaaccgcta ctgtaccaac caagacaggt tgaccctttt ggtaacgagc cttaacgtct
     7141 tcgacaaccg ctttaaactt agattctata cttgcataaa gaaggtctga gtggtcaata
     7201 cgttgaacag gacggtttgt tgggattgga ataacacgaa tgttgtagat ttcacggaat
     7261 tcttcttcct cagtcttacc tgtacccgtc ataccagaca atttcttgta catacggaaa
     7321 aggttttggt acgtgattga ggcagatgtc ttggtttcat cctggattgg cacaccttct
     7381 ttggcttcaa tagcttggtg caatccatca gaataacgac gaccttccat ggtacgacct
     7441 gtaaattggt cgacaatcaa gatttcttgc tcttcgctca ccacatagtc aatatcgaga
     7501 agcatgatgt agttggcacg aagggcgtta tcgataaagt gggtcaaagc cacgttttcg
     7561 atgtcataga ggttttcaag tttgaagtag ctttcagccc tgtcaatccc tgaatcagac
     7621 aaaccaatag tcttagactg cacatcgatg atgtagtcat ctttgttcaa agattttaca
     7681 tagtggtctg ccatgtgata caactgactg gtttcaaccg cattggcacc tgatacaatc
     7741 aaaggtgtac gagcctcgtc aatcaagata gagtcaacct catcgaccaa ggcatagtta
     7801 agcggacgtt gtaccatgtt ttcagcgcga acgaccatat tgtcacgaag gtagtcaaat
     7861 ccgatttctg agttagttga gtaagtaata tcacactcat aggcttcttt tttctccatt
     7921 ggagatttgg tagccaagtt aatccctact gacaaaccaa gccaagagta caattcaccc
     7981 atctcagtcg cgtcacgttc tgacaggtat tcattaaccg taactacgtg aaccccttta
     8041 cctgaaaggg cattgaggta taccggcatg gtcgcagtca aggttttccc ttcccctgta
     8101 cgcatctctg gcacgtcacc atggtgaaga acaatccccc ccatgacctg aaccttataa
     8161 gggaagagac ctaggacacg tttggcacct tcacggacaa ccgcaaatgc ttcgtaaagc
     8221 aatgaatcca gtgattctcc attttgataa cgttccttaa attcaactgt ttttgctttt
     8281 agttggtcgt cagtcaaagc agccatttgg tcttcgtatt tgaaaacctt gtcagccatc
     8341 ttttccagac gacggatttc tcctttatca ttttcgataa ttgtttttaa aatattagcc
     8401 atgtttttcc ttactttaaa ttccgaatat tttagaatgt tcctttaatt ttagcacaat
     8461 tactgattat tttcaaggaa gaatccccat tttgaaaagg aaaaagaggt tagtttccaa
     8521 gctaacctct ctgactttta tgatacttta attgccaaga atcggcaaaa tagcaaatag
     8581 gataaataag ttaatccaaa gagaaagaca gcagatcaat ccaaaaacaa agagactgac
     8641 tttcttggac agcaaggcca tcaaaatgga cagaatgcca aaaactagca agactttctg
     8701 tatgctgaag aggtcaatct tcccccccca gaatcggact gccccaagga agaaagaaga
     8761 aagcaatcca gatcaacaga actaaaccaa tatagacctt agaatagcgt gtaataaatg
     8821 attttagatg tgccataagc catctcctag gattgtatat cctttacatt tgtatatgtc
     8881 cattttcgac aactaataca tctgtacaaa ggtctgaaat atcttccgat atgtgcgacg
     8941 tcaggataac cagaccattt tcttttttca ggtaagataa aatgacctgt ttggttaacc
     9001 tcacactttt ctcatccaaa gcattcatag gttcatcgag aaagagtata gaaggagagg
     9061 aaataaaggc ttgaatcaag gccatttttt gctttgttcc caaggataaa tgccggtatt
     9121 caacgtcttc aaattcctgt aaatcatagt attgaatcca ataggcaatt cttttttccg
     9181 taactttaga tgaaaaatac cttaacagtt ccaaattttc tctcagggat aaatgagata
     9241 aaaactcgac tttttcaatt aaaattcctg catcctgaat ataatgattt tttatcccgt
     9301 aaactttacc atcttgaaga acttttcctt tgtcaagctt aatataacca gcaagtatct
     9361 ttaaaagaac cgtcttgcca gatccattat cacctttaag tccataaatt tttcctgatt
     9421 gaaacaccaa attcaactga tttaaaatag gtcttttccc ataaattttc tgtccattta
     9481 taatttcaag tctcatcgtt tatcctttct taaatttctc ttccaagcga ctaagaatca
     9541 acataactaa taagcttaag agtactaaat acactgttac aatatgatcc atcatccatt
     9601 cttgtcccac aaatgccaaa aaatagagaa aaaaagaaaa accagcgccc gcactaatca
     9661 gtgctatcag ataagacagt agtaagtaca acatcattaa ctgagcaagt aaaaagaaaa
     9721 gtgcgactgt tttgaaatga attcctagcg gaagtaaacc aagaaaccag acaaacaaat
     9781 cttgagtaaa aaatttagaa atgacaacca atcgtttcct tgtataatcg aaaaaggagc
     9841 ttgattgata gcgaatcaag tgatggtaat ggtctgccaa atcactttca agtaaaatga
     9901 cagagagcca tcctaccatg aaaagcgaaa ggtttaacaa gagtttgggg atttctaaat
     9961 tcgcttcaga atagtcatta taggaaaaaa ttgaaatcaa ttctctcttg aaaaagataa
    10021 cagcatcctg cttttgaacc gctgttgaca atagatagtt tatcaaaatc atcatcagaa
    10081 ctatacgaag tagtaaaacg atctttctct ttagccaaat attgttaaaa ttcttaaaca
    10141 ttatcttcat attttttaag taccatataa gggacaacat aaaggatata aggagtaaag
    10201 agtttcatca aacttacatc cccatagcta gcttgcatca aactcgtcat aggaaccata
    10261 tagtaaggca aggcactata gagcagcata gaaccaagcc aaagaaacat caaataggtg
    10321 attgccgaac gagtcacatt ccccacataa tcaactattt gtaaaaaata gattgcattg
    10381 atgaaaatac cgattagtag gacacaagta aagaacaaat agctttttat ctctccatct
    10441 aggagtcttt gtaaaccact cccatcagaa aatagagaat tccattcaag aggagaaaaa
    10501 gtcccaaaga aataggttat aattgcaatt atcagcacag tcactaaata tatcaaacaa
    10561 gggatacttg caacttgcaa agtcaacttt ctttttaacc cttgatagct cacttctctt
    10621 ccaatactca atcgtaaaac tttatttttc agctcaatat attgaaaacc tagcaaaaca
    10681 atgatgattg gaataataaa tgattgataa aaacctagat tgaactggag aatacgaaaa
    10741 tctaatgctg attttaataa gtcattttta attgcttgtc catcgaattt agctagatcg
    10801 ggcgcatgaa tatcaaaaaa gaaataagtc atcaaaacat cacgataaac caaagcaaga
    10861 aaaagcaaag taagcaagac ataaacaaat ttatgccttg ccaaagttaa gagaggaatc
    10921 ttcataagcc acctcctaca aatagaaagt aatataatac tcaatgaaaa tcaaagagca
    10981 aactaggaaa ctagccgcaa gttgctcaaa gcactgcttt gaggttgtag ataagactga
    11041 cgaagtcagt tacatatata atccaaggcg acgttgacgt ggtttgaaga gattttcgaa
    11101 gagtataaat caatgccttc cgcctttaga cttccctagt tcccgtctca agtcaagcat
    11161 ttttttgaaa cagatatagt ctaaccagtc actagcaaaa gcgataagta gtaataatag
    11221 ccaaataccc cataacttga tatctgtcaa atttctcaag acgatattga aaaacagaac
    11281 tgaaaaaact gtccaagcaa ggctaaaaag agcatagagg gtgatgtaaa tccagtaaaa
    11341 atagtaaaaa attgggaaaa tcttactatc tctgtttgcc ttttcaatcc agctgaaaaa
    11401 gtaaaaacag ggcaataaga ttatcatttt aaacaagtat tccatcacaa acctcctctt
    11461 ttaaattgtt ttctttagtt tcgttctatt aaatgttatt accaatataa accttggaat
    11521 agcgtgtaat aaatgatttt agaggtacca taagccacct cctacaaata gaaaccgata
    11581 taaatcaatg ccttccaccc ttagacttcc ctagttcctg tctcaagcga aacatttctt
    11641 tgaaacagga ataagttaac caattcatac caatagctag cagaataaaa agaaaccaaa
    11701 tgccccataa cttgatatct gtcacatttc tcaagacggt attgaaaaac agaactgaaa
    11761 caactgtcca agcaaggcta aaaagagaat agaaggggat gtaaatccag taaaaataat
    11821 aaaaaattgg aaaaaactta ctatttctgt tggccttctc aatccagtta tcaaaataaa
    11881 agtacggtgc taaaagtaag aatttaaaca aatgttccat caccgacatc accctccttc
    11941 ttttgatagc gttttctatt attttattat atcaaaaaaa tccggaactg tcattccaga
    12001 ttctactttt ttatttgcgt tttcttgcga tgagatgaat cggtgttccc tcaaaaacaa
    12061 aggccttgcg gatttgattt tccaagaaac gcaggtaaga aaagtgcatg agttcttctt
    12121 cattgacaaa gatgacaaag gttggtggtt tggttgccac ttgggtcgca tagaaaatct
    12181 tgagacgttt tcctttgtct gtcggtgttg ggttgatggc aatggcatcc atgatgacat
    12241 cgttcaagac agctgatgga atacgtgtat tttgactttc gctgatttgc ttaatcatct
    12301 caggaagttt gtggagacgt tgcttagtca aagctgatac aaaaataatc ggtgcataag
    12361 gcaggtattg gaactgctca cggatatctt cttcccagtt tttcatggta tggttgtctt
    12421 tttcaagcgt atcccacttg ttgactacga taatcattcc tttaccagct tcatgggcaa
    12481 atcctgcgat acgcttgtcg tactcacgaa tgccttcttc cgcattgatg accatcaaga
    12541 ccacatctga acggtcaata gcacgcatgg cacgcataac agagtatttc tcagtatttt
    12601 cataaacctt accagactta cgcataccag ccgtatcaat catggtaaac tcttgaccat
    12661 ctgtatctgt aaagtgggta tcaatagcgt cacgagttgt tccagcaaca ggactggcaa
    12721 tgacacggtc ttctcccaag atagcattaa tcaagcttga ttttccaacg ttaggacgac
    12781 caatcaagct aaacttaatg acatctggat tttcttcttc atattcattt ggaaggtttt
    12841 ccacaatcgc atctagtaca tcccctgtac cgataccatg gacagatgag ataggcagtg
    12901 gttcacccaa accgagagca tagaaatcat agatatcatt tctcatctca gggttatcca
    12961 ccttgttgac tgcgaggata actggtttgt gggtcttata aagcttgcgg gctacgtatt
    13021 cgtctgcatc ggtaattcct tccttaccgg acacgacaaa gacgataaca tctgcttctt
    13081 ccatggcaat ttctgcctgg tgcttgattt gttccatgaa aggagcatcg acatcatcaa
    13141 ttcctcctgt atcaatcatg ctaaaagaac gattgagcca ctcacccgtt gcataaatac
    13201 ggtcacgtgt cactccttcg acatcttcta caatggagat tcgctcacca gcgatccgat
    13261 taaatagggt tgatttccca acattgggac gtcctacaat ggcaatagtt ggtagggcca
    13321 taatttctca ctttctacaa taatttcttc tgttcaagat tttttctagt tgagcttggt
    13381 tcagcttgac caaactgttc tgctaggcgc tgactccagc ttgtggtcgc acgcccccca
    13441 gcatagtcag cctgcacacg gtcataagct tggattgtct cagttgactg ttcttggtat
    13501 tcttcctcaa agacaacatt ctctagtggc agtctcggtt tcacatcatg atgttgattt
    13561 ggcacaccca gtgccatccc aaagacagaa taggtgtagt caggtaggtt aaagagctct
    13621 gccacttctt cagacttgta tcgaaccaaa ccgataatca caccaccata gcccaagctt
    13681 tcagctgcca acaaggcgtt ttgtccagca agagctgcat cgaccgaact aatcaagaga
    13741 ccttccacac cttggggttg gaaggtgtcg gtatgaagtc gggctccctt ttctgctcgg
    13801 ttcaaatctc cgacaaagag aaggaaaaca gcagactggc gaatggcttc ttgaggtacc
    13861 aattcataca aggcatcttt cttctcttga cttcgtacca caatcacaga gtaggattgg
    13921 aaattcttcc aagatgatgc catctgggct gctgtcaaaa tctcatttaa gtctacttgg
    13981 ggaatttctt gctctttaaa cctgcgcact gaagtatgag ccttcatcaa tttaatggtt
    14041 tctgtcatcg acggtttact ccttctaaac gagtctcctc agccaaataa cggatgcgtt
    14101 ccatgacccg tctggcttcc caggtttcgt catttccatg tttcactttc gcaaaatgct
    14161 tctccaaatc ttcaaagttg aagttggatg tgaaaaaggt cggtaaattt tcctgcatcc
    14221 gatattggag aatgacctgc aggatttcgt cacgtaccca aacggttgat tgctcggcgc
    14281 caatatcatc taaaatcagg acctcagaca gcttaatctc atccaccaag gtcttaacat
    14341 tgccatcact gatagcattt ttgacatcaa tgacaaagct aggatagtgg aggagagttg
    14401 atgaaacacc acgtttttct gataaatcat gagctaaggc cgccaccatg aaacttttac
    14461 ccacaccaaa gtctccatat aagtaaagac cttttcgaat agctggatat tgctccacga
    14521 aggctagtag cttttcaaaa actggtaagc gccccaaatc atccaagtca acttgagcca
    14581 aactagcttt cttgagactg gctggtagat tgattaactt gagacggttc ttaatagccg
    14641 cttctttttc agccgcgatt agctcaggag tttcttcata tgaaacatct gcatagccat
    14701 gattcttaac caaaatcggc ttgtagcctt tggcaatata atccgtatcc ccacggagaa
    14761 acttgtcacg ctcggtgatg tactgattaa acttggagat actgcgattt aattcctctg
    14821 gagtcaagga ttcttgctgg ataaaagccg caacatcagg gtccttcatg attttctgga
    14881 ccaaatcttg ataataaaaa cggctgggtt gacgtttgag tacgtctccg acactttcca
    14941 tctaatctcc tcctttttct aatcgagcta atagttcttg cttcttacgt tctagttcca
    15001 gacgagtttc ctcgctggtt tcattcttat attcaggatt actccattta ggaacattgg
    15061 tttttactgg ggcagtctga ttctgttttt gtgtttttgc tttctgtcct cgctcacgga
    15121 tgcgcaagac tgccttttct gccgaatgaa tcttttgata ggcatagtca ttggctacct
    15181 tcatggcata tttctcattg atatttgccg aatcgacctt attaaaggtc aataaaagaa
    15241 tgatattgat gacttcgtcc agcaagccca agccagccat ctgttgcaag agttctcttt
    15301 ctgtttgggt aatggttccc ttgcgtgttt gcttgatttc tgccaagaac tgcagggcag
    15361 ttttactttt ggcttctttg ataatggttg tttccttaag actaaagtca gaggaaactg
    15421 gtttttgagc aattttttca cgcatgcgtt tggttgaaat aacctgagaa acagctgttg
    15481 acttggccaa ttgataggtt tcaaaccaag tccatttctt ctcctcggca atagcaaaga
    15541 ggtttaagac atcggactgc tcatccgcaa aacgaagtcc atctcgagcc atcagctggc
    15601 gaaaatgttc caagtcaaaa tcattggcca ctttcttctt gagaccaagg tcttcttgac
    15661 tgcctagttc tgccaattct ggaaagactt gattgagtga gacaggtatt tcttcaccat
    15721 cagcactttc aactttcaaa tcctccacag cagagtcgcc aatctttttc tctaagagtc
    15781 tgcgataaac aggatgctcc aagaagtctt gactagatag aggagcatgg agggctagct
    15841 gataaacatc ccccttttga tagagggtca agagattaaa agcagataag attttcaatg
    15901 attttatcag tctatccatc ccaaagttga gatgattgag gatacttgaa aaaagatatt
    15961 cctttctacc attatcccaa aaactgattg tataaagata aaggctcagt gcctcctgac
    16021 cgataatcgg gaggtagcac tgtaccagag atgaggtatc ttgcgacacc cgattattct
    16081 ttagataaga aaaacggtca attggcttca tttatctttc ctttttcttt ttagaggact
    16141 gggtgatttg ttggagcaag ctctctaact cactgacatc cttaaaacta cgatagacac
    16201 tagcaaaacg tacataggta atctcgtcca attcagccaa ctcctccatg acgagtgaac
    16261 caatgtcctc actttgaatt tcattttcat ttcgaccacg gagtttctgt tcgatacgat
    16321 tgactaccat gttgatttca tcacttgaca caggacgttt ctgggctgag cggataatcc
    16381 cattaaagat tttatctctg gagaattgtt cccgtgtacc atctttttta acaaccacta
    16441 aggttctttc ttctactcgt tcgtaggttg taaaacggtg ttggcattcg tcgcactcac
    16501 gtcttctacg aatggtgttc ccttcttctg cttggcgact atcgataaca cttgacttgg
    16561 tagccccaca ttttggacaa cgcatccttt ccctccttat cgttttcttt tcattatacc
    16621 attttttaag cgattcccaa aacaattctt ctttttgctt gacaagtttt ttgttttgtt
    16681 gtattattta attaagacaa aaggataaga gaaaggagac taagatgtcc tggacatttg
    16741 acaacaaaaa acccatctat ttacagatta tggagaaaat caagcttcag attgtttccc
    16801 atacactgga acccaatcaa caacttccaa ccgtgaggga gctagctagc gaggctggtg
    16861 tcaatcccaa taccatccaa agagccttat cagaccttga acgagaagga tttgtctaca
    16921 gcaagcgaac aactggacga tttgtgacta aggataagga gctaatcgcc cagtcacgca
    16981 aacaattatc agaagaagaa ttggaacact tcgtttcctc catgacccat tttggctatg
    17041 aaaaagaaga actaccaggc gtagtcagtg attatattaa aggagtttaa gcctatgtca
    17101 ttactagtat ttgaaaatgt atccaaatca tatggagcaa caccagccct tgaaaatgtt
    17161 tctcttgaca ttccagctgg aaaaattgtc ggccttcttg ggccaaacgg ctcaggaaaa
    17221 acaaccctga ttaaactaat taatggcctc ttacaaccag atcaaggacg tgtcctcatc
    17281 aacgacatgg acccaagccc agcaaccaag gccgttgtag cttatttgcc tgatacgacc
    17341 tatctcaatg agcaaatgaa ggtcaaagaa gccctaacct acttcaagac cttctataaa
    17401 gatttcaatc ttgaacgcgc ccatcatcta cttgcagacc tgggcattga tgaaaatagt
    17461 cgtctcaaga aactatcaaa aggaaacaaa gaaaaggttc aactgatttt ggttatgagc
    17521 cgtgatgctc gtctctatgt tttggacgaa cccattggtg gggtggatcc agcagcccgt
    17581 gcttatatcc tcaataccat tatcaacaac tactcaccaa cttctaccgt tttgatttct
    17641 acccacttga tttctgatat cgagccaatc ttggatgaaa ttgtcttcct aaaagacgga
    17701 aaagtcgtcc gtcaaggaaa tgtagatgat attcgctacg agtcaggtga atccattgac
    17761 caactcttcc gtcaggaatt taaggcctaa gcaaaggaga ttatttatgt tttggaattt
    17821 agttcgctac gaatttaaaa atgttaacaa gtggtattta gccctctacg cagccgtgct
    17881 agtcctttct gccctcatcg gaatacagac acaaggcttt aaaaatctac cttaccaaga
    17941 aagtcaggct actatgctac tttttctagc tacagtcttt ggtggcttga tgcttacact
    18001 tgggatttca accattttct tgattattaa acgcttcaaa ggtagtgtct acgaccgaca
    18061 aggctatctg actttgacct tgccagtttc tgaacaccat atcatcacag ccaaactaat
    18121 cggtgccttt atctggtcat tgattagcac cgctgtattg gctctaagtg ctgttattat
    18181 tctggcttta acagctccag aatggattcc tctttcttat gtgattacat ttgtagaaac
    18241 acatctccct cagatctttc ttacaggtat atccttccta ctaaatacta tttcaggaat
    18301 cctctgcatc tacctggcta tttccattgg acagcttttc aatgaatacc gtacagcact
    18361 cgctgttgca gtctacattg gtatccaaat cgtcattgga tttattgaac ttttcttcaa
    18421 tcttagttct aatttctatg tcaattcact ggtaggactc aatgaccatt tctatatggg
    18481 agcaggtata gccattgttg aagaactcat attcatagct atcttttatc tcggaaccta
    18541 ctacatcttg agaaataagg ttaatttgct ttaaataatt tttacctaga tatgtaacat
    18601 actcatagaa caaaagagac caggcaaaaa gtctttacaa ttagaaaacg catagtatca
    18661 agtgttaaaa atccttgata ttatgcgttt tttgtgagct gacttatttc ctttcactat
    18721 atcgcaaaat gaaataagaa cggaacgatg ggattttgga attcaaatca atttataaga
    18781 atgttttaga agtaatatta tcctattcca gattcagttc actatacaat tgagttttca
    18841 agcaacctgt ttacataatg tgtacataat taggttcgtg attccaccct tttcaccttt
    18901 aaaaacctcg ctttcgcaag gctcttctat ttataagata aggcacgttt aaaggttttc
    18961 caaatcccta aatcatccgt ttgaagaacg agactagcat acatgcgtcc gataaatcct
    19021 gttgctacca ccgcaaaaat cactgtaata gcaagtgaaa tccatgcttc cgctccctcc
    19081 gcatagccat taatcgttcg aaacggcata aagaaggtcg aaataaaggg aatataagaa
    19141 ccaatcttca agaggagatt gtcaccagct gcacctagag ctgtcactcc aaaaaaacca
    19201 cccataatca aaatcatcaa aggcgacaag gctttccctg agtcttcagg acgagaaacc
    19261 atagatccta ggaaggctgc caagactacg tacatgaaaa gactgatcaa aataaagagc
    19321 aaggtattca gtgagatagc atctcccaag tgatccaaaa taccagactg agccaagaat
    19381 ggcaaatctt taaagagcaa aacggcagcc agaccaccta caacatagat cccaatatgc
    19441 gttaaaatca ctagaaacag agccatcatc cgcgcataga aatagtgact tgcccttatg
    19501 ctagaaaaga cgacttccat aattttggtg cctttttcac tggcaacttc ctgagctgtt
    19561 acacccgcat aggtaatcag aatcatataa agaaagaatc ctaaggcacc tgctgcaatt
    19621 gtttgaataa actttttatt ttccttggct tcatcaatct tttctgtgaa ttgaattgtc
    19681 tgcgctaagc gtttttcctg ctcttgagac aaggaagcag ttgaacgatt aagctgattt
    19741 tgcagttcat tgagtgtacc tgtaacctca aatttaattc cattttcaag cgatgtttcg
    19801 ccatgataaa ctgcctttag aacactatct tcttgatcaa tggtcaaata accttttaat
    19861 ttttcttctt taattgcttc tttggcactt gcttcgtctt tatagtcgaa gttaacacca
    19921 tttacattct tcagtccttc tgctacagat ggcactgttg tcactactgc cactttatta
    19981 tttttagcca tagaagaacc ttggagatgc ccaattccta cagagattcc taaaaagagg
    20041 aacggcgaaa tcaccataaa gaagaaactc catgactcga catgtcgaag ataggtttcc
    20101 ttgattacaa cccacatatt tctcatactt ccactcctga ttctagttta aagatttcat
    20161 cgatagttgg cgcttgttgg tcaaatgttg cgatatattg accttgagtc aagattggga
    20221 agagttccct tccagcgctc tcatcctcca aaatcaattt ccaactgcct tgtttggtca
    20281 agctcacctg tttgacatga ggaagatttt ccaattcttc cttgcttcgt tcacttgaaa
    20341 caaagagacg cgttttcccg tattgattgc ggacatcctg aactggtccg tgcaagacca
    20401 cacggccatc tcgaatcatc agaatatcgt cacaaagttc ctcaacattg gtcatgacat
    20461 ggtcagaaaa gataatggtt gctccgcgct ctttttcctg aaaaatgact tgtttgagca
    20521 attctgtatt aactgggtcc aatccactaa aaggctcatc caagataatc aggtctggtt
    20581 catgaatcag agtaataatg agctgaatct tctgctgatt tccttttgac agactcttga
    20641 ttttatctgt cagctttcct tttacttcca acctcttcat ccattgaggg agtttttctt
    20701 tgacttcttt ggcatccatg ccttttagag tcgccaagta gcgaacttgt tcaagaactg
    20761 tcaatttagg catgagactg cgttcttcag gcagataacc aatccgagca tagatctcct
    20821 gacgaatatc ctgaccatcc agaccgattt ctccctgata ttctaggaat tttaaaatac
    20881 tatggaaaat cgttgttttt ccagcaccat tttttccgac tagtcccaaa atacgacctg
    20941 gtcgcgcttg aaagtcaata ccaaacaaaa cttgcttgga tccaaaactt ttctctagac
    21001 ttcttacttc tagcatcttt cacctccgaa atttcttgca ctcattatac tcctttttga
    21061 tagcctttac aatgtttttt gtccattttt agaagactat tgctgtgtaa aatatggcct
    21121 ggagcacttt tatactcaat gaaaatcaaa gagcaaacta ggaagccagc cgtagactgc
    21181 tcaaagtaca gctttgaggt tgcagataaa actgacgaag tcagctcaaa acactgtttt
    21241 gaggttgtgg atagaactga cgaagtcagt aactatatct acggcaaggc gaagctgacg
    21301 tggtttgaag agattttcga agagtattag tgataaatcc attatacagc agcaaactta
    21361 atttatacct tccgctcctc aactgtctat ttttaatcct gaattgttat ttgagtaact
    21421 cctttttcct cgtaaagttt tcttcctcta aaacttctgg aaaaaggcta atagtttcag
    21481 acaacatttt tataagaaac aagttcatct gtcatttcaa gaaggagtaa tcctttatct
    21541 actaatggac ggaacagaat tcaaccgctt gtcccatatg ttttctaagg attatatagt
    21601 aaaatgaaat aagaacagga caaattgatc aggacagtca aattgatttc taacaatgtt
    21661 ttagaagtag atgtatacta ttctagtttc aatctgctat atctattatg cacaccccta
    21721 taggatctaa tgaaaatcac aacaggctca ttcatagata gttacctcag gaaactaaga
    21781 aaacgactac aaaggaagtc gcattcatcg aaaagtggat taacaactat cctaaaaaat
    21841 gcttgaacta caagtccccc agagaagact tctggatgac taacttgaac ttgaaattta
    21901 gcaataatta attcaccatc taactatatt tagtaattat ttcagaactg attaatatta
    21961 aaattaacta acaattcaaa ggattcatac tagccataaa ttacgtccat cagagagaga
    22021 ctcttactac ttttagattt tagtctttct agcttcagaa tacatctaaa ctttagggaa
    22081 aatgactatt cgaaagcgcg aatgcctcaa aattatctca gataagctat tcgaaactta
    22141 gaatgctttt aaatttatgg aattgcgatt atttgaaacc tagaatgcat ataaccttta
    22201 gttgacagac ctattctaag tctcgaaggg ctatttactt tctattcctt atcaaaaaag
    22261 actcattccc cctttctcct ccaaaatatg gtatagtaga aatatactat ctatgaggag
    22321 tttacatgtc acaggataaa caaatgaaag ctgtttctcc ccttttgcag cgagttatca
    22381 atatctcatc gattgtcggt ggggttggga gtttgatttt ctgtatttgg gcttatcagg
    22441 ctgggatttt acaatccaag gaaaccctct ctgcctttat ccagcaggca ggcatctggg
    22501 gtccacctct ctttatcttt ttacagattt tacagactgt cgtccctatc attccagggg
    22561 ccttgacctc ggtggctggg gtctttatct acgggcacat catcgggact atctacaact
    22621 atatcggcat cgtgattggc tgtgccatta tcttttatct agtgcgccta tacggagctg
    22681 cctttgtcca gtctgtcgtc agcaagcgca cctacgacaa gtacatcggc tggctagata
    22741 agggcaatcg ttttgaccgc ttctttattt ttatgatgat ttggcccatt agcccagctg
    22801 actttctctg tatgctggct gccctgacca agatgagttt caagcgctac atgaccatca
    22861 tcattctgac caaacccttt accctcgtgg tttataccta cggtctgacc tatattattg
    22921 actttttctg gcaaatgctt tgacacgtaa aaaatccgtt tggtttccca agcggatttt
    22981 gtgctttatt ttgaaacttc ttttgcaaga acaaagttcc caagtgtggc agaaccattt
    23041 cctgcgactg ctggcgtcac gatatagtca cgcacatctg gtactggtag gtaaccatta
    23101 agaagagatg taaatttctc acggacacgg tccagcatat gttgttgagc catgacccct
    23161 ccaccaaaga caatcacgtc tgggcggaaa gtcactgtcg cattaaccgc agcttgagcg
    23221 atatagtagg cttgaacatc ccaaacaggg ttgttgagtt caatagtttc cccacgtaca
    23281 cctgtacgag cttccaaact tggaccagct gcatagcctt ccagacatcc cttatggaaa
    23341 ggacaaacac ccttaaactc tttttcaata tccattgggt gtctagcaac ataataatga
    23401 cccatttcag ggtgacccac accaccgata aactcaccac gttggatgac gcctgcaccg
    23461 atacctgtac cgattgtgta gtaaaccaag ttttcgatac gaccaccagc attgttacgg
    23521 gcaaccactt caccgtaagc agagctgttt acgtctgttg tgaagtacat tggcacgttt
    23581 agggcgcgac gaagggcacc aagcaagtcc acatttgccc agtttggttt tggagtcgtc
    23641 gtgataaagc cataagtttt tgagtttttg tcaatatcaa tcggcccaaa tgaaccaact
    23701 gcaagaccag caaggttatc gaattttgag aagaactcaa tggttttatc gattgtttcg
    23761 attggagttg ttgttggaaa ttgtgttttt tctacaacgt taaagttttc atcaccaaca
    23821 gcacagacaa actttgtacc gccagcttcc aagcttccat ataattttgt catgataaac
    23881 ctcttgtttt tattttcttt attatagcat acttcgaaag tctaaatgtc tctatttttt
    23941 agattttcct ctgtaaatct tactatctaa taaaaacgaa caaacatgtc atttgttcgt
    24001 tttcacatta gagaggattg attagatttt cacttcgatc acagcatccc ccttcgcaac
    24061 tgaacctgtt gcgactggag ctactgaagc gtagtcagct gtatttgtaa cgataaccat
    24121 tgttgtatca tcaagtccag ctgtagcgat tttatttgag tcaaatgttc caagaacatc
    24181 gccaactttc accttatcac cttgagcaac ttttgcttca aaaccgtcac cgttcataga
    24241 tacagtgtca ataccaacat gaatcaaaac ttcagcacca tttcttgttt tcaaaccaaa
    24301 agcgtgccct gttggaaagg caattgaaac ttcagcatca gctggtgcat agaccacgcc
    24361 ttggcttggt ttcacagcga taccttgtcc catagctcca cttgagaaga ctgggtcatt
    24421 gacatcagca agagcgacaa catcaccgac gataggagtt acaagtgttt cattttgaag
    24481 agctgctggc gcaacttctt ctttttcttc agccacttca gcttgttttg cagctgcagt
    24541 tgcgtctact tcatcttcgt aaccaaacat gtaagtaaga gcaaaaccaa gggcaaatga
    24601 tacagctacc ataagaaggt attgtggaag ttgtccgtta ccaacataaa gcattgtacc
    24661 agggatgatg gtgataccat taccagtacc agcaagtcca aggatagaag ccaatccacc
    24721 accgattgca ccagcaatca atgaaaggaa gaatggttta cggaagcgca agttcacccc
    24781 gaagatagca ggctctgtaa tacctaggaa ggcagaaaga gcagccggga aagcaagtgt
    24841 tttcagtttt ggattttttg ttttaacacc aaccgcaaca gtagcagcac cttgagctgt
    24901 catagcagct gtgatgatag cgttgaatgg attagcatgg tcagcagcaa gtaattgcac
    24961 ttcaagcaag ttgaagatgt ggtgcacacc tgacacgacg atcaattggt gaaccccacc
    25021 aatcaagaaa ccaccaagac caaatggcat gctaagaatc gcttttgtag caataaggat
    25081 gtagttttca acaacgtgga aaactggtcc aatgacaaag agtccaagga tagacatgac
    25141 caaaagtgtc acgaatggtg ttaccaagag gtcaatgaca tctggaacaa ccttgcggac
    25201 agctttttca aatttagctc cgacaacccc gatgatgaag gctggaagaa cggaaccttg
    25261 caaaccaaca acagggatga aaccaaaaaa gttcatcgct gttacttcac caccttgagc
    25321 aactgcccaa gcgtttggaa gtgagccaga gacaagcatc ataccaagaa cgataccaac
    25381 ggcaggattt ccaccaaata cacggaaggt tgaccacaca accaaacctg gcaagatgat
    25441 gaaggctgta tctgtcaaga tttgtgtgta agttgcaaag tcacctggaa gtggcatttc
    25501 aagagcgttg aaaagaccac gcacacccat gaagagacct gtcgctacga taactgggat
    25561 gattggaacg aaaacatcac caaaagtacg gatagcacgt tggaaccagt tcccttgttt
    25621 agcaacttct gctttcatgt catccttaga tgatgttggt aatccaagta caacaacttc
    25681 atcgtacatt ttgttaactg tacctgtacc aaagataatt tggtattgcc ctgagttaaa
    25741 gaaagcacct tgaacttttt ccaagttctc aatcacttct ttattgattt tctcttcatc
    25801 tttgaccatg acacgtagac gagtcgcaca gtgggcaaca ctattgacat tttcacgtcc
    25861 gcccaaggca tcgatgactt tttttgcaat ttcctgattg ttcatttgca aaaatctcct
    25921 tatataacat tttgttcttg tttgaaagcg attttattcg cccttacgac tattatttta
    25981 tcatgtttta aaaatatgtc aagcgttttg cataaaaata ttctatccac ttagtgggca
    26041 agctttagat tgattgtttt tgactgtttc atcaggaatt ccttgaaaaa taagtttgaa
    26101 accttggtta aaaagtattt tatttcatta tctttcacat tttcgtaaaa atttgattga
    26161 tttcctgtat ttattttatg ataatcgttt gacagttttt cctctttttt aacataaaag
    26221 gcttgcattt tttatcgaaa acagttacta ttaaaagtga taagattttt ggaggaatga
    26281 atggaatgga caactgagcg tcgttacaga ctttatcaag attggacgca agaagaaatt
    26341 caacatataa aggaaaatat ggcacaatct ccatggcata ctcattacca tgttgagcca
    26401 aaaacaggac ttctcaacga cccaaatggc ttttcttact ttgatggcaa gtggatcctc
    26461 ttttaccaga attttccttt tggtgcagcc cacggtttga aatcttgggc acagctagaa
    26521 agtgatgatt tggttcactt taaagaaact ggaatcaaag ttttaccaga tactccatta
    26581 gatagccacg gtgcctactc tggttctgcc atgcaatttg gcgataactt attcctattt
    26641 tatacaggaa atgttcgcga tgaaaactgg atccgtcacc cataccagat cggtgctttg
    26701 atggacaagg agggtaagat tacaaagatt gacaagatct tgattgacca gccagcagac
    26761 tctactgacc acttccgcga tccacaaatt tttaacttta aaggtcaata ctatgctgtc
    26821 gtcggtggac aggacttgga gaaaaaaggc ttcgtccgtc tctacaaggc tgtcaataac
    26881 gactacacaa actggcaagc agttggcgac cttgactttg ctaacgaccg tactgcctac
    26941 atgatggaat gtcctaatct gatctttgta gaggaacagc ctgtccttct ctactgtcca
    27001 caaggattgg ataagaaagt tctagactac gataatatct ttccaaatat gtataagatt
    27061 ggggcttcct ttgaccctaa aaatgccaaa atggtagatg tgtctcaact tcaaaacatg
    27121 gattacggtt tcgaagccta tgcaactcaa gccttcaacg ctcctgatgg gcgtgctcta
    27181 gcagttagct ggcttggttt gccagatgtt tcttacccat ctgactgttt tgatcaccaa
    27241 ggaaccttct ctttggtcaa ggaactcact atcaaagacg acaagctcta ccagtatcca
    27301 gtcgctgcta ttaaggacct tcgtgcttct gaagaagcct tctcaaaccg ttcccaaacc
    27361 aagaacactt acgaacttga actcaacttg gaagctaata gccagagcga gattgtctta
    27421 cttgctgata aagaaggtaa gggactttca atcaactttg accttgtaaa cggtcaagtg
    27481 acagtggatc gtagccaggc tggagaacag tatgcccaag aatttgggac aactcgttct
    27541 tgccctatcg agaatcaggc tactactgct acaatcttca tcgataactc tgtctttgaa
    27601 attttcatca ataaaggaga aaaagtattt tctggtcgtg tcttcccaca tgcggaccaa
    27661 aatggtatcc tgatcaaatc tggaaaccca actggaactt actatgaatt agattatggt
    27721 cgcaaaacta actgatgtcg ccaaacttgc aggcgtcagt cctactaccg tttctcgggt
    27781 tatcaataaa aaagggtatc tatctgagaa aaccatccaa aaagtcaatg aagccatgag
    27841 agaattaagc tataaaccca acaacctggc tcgtagtctg caaggaaaat cagctaagtt
    27901 aatcggcttg attttcccca atatttccaa tgttttctat gcagaattga ttgataaatt
    27961 ggaacaccaa ctcttcaaaa atggttacaa gaccatcatc tgcaacagtg aacatgattc
    28021 tgagaaggaa cgcgaataca tcgaaatgtt ggaagccaat caggtagacg gcatcatttc
    28081 tggtagtcac aacctaggaa tcgaagacta caatcgtgtg acagcgccga ttatttcctt
    28141 tgaccgaaac ctatcgccag acatccctgt tgtctcctct gacaactatg ctggtggggt
    28201 tcttgctgcc caaaccttgg tcaagacagg tgcccagtct atcatcatga ttacagggaa
    28261 tgacaattcc aattcgccaa ccggactgcg ccacgctggt tttgcatccg tactcccaaa
    28321 agctcctatt atcaatgttt ccagtgactt ttctcccgtc agaaaagaaa tggaaatcaa
    28381 gaatatcttg acccgggaaa aaccagatgc catttttgct tcggatgatt tgacagctat
    28441 tctgatcatt aaaatcgctc aagaattggg catttctgtc ccaaaagagc tcaaggttat
    28501 cggctatgat gggacctact ttatcgaaaa ttactaccct caattggcta ctatcaagca
    28561 acctttggaa gagattgctt gtctcactat tgatcttctc ttgcaaaaga ttgaaggcaa
    28621 ggaagtcgcc acaactggtt acttcttacc agttacacta ttaccaggaa aaagtattta
    28681 aacacaagaa aactcagacc gattcgtctg agtttttatg atcttaaatt ttcgagatag
    28741 cgctgggctg tctctaggtt aaaggtttta tctgagatga ggcgctctac taggggagca
    28801 acttcagatt cactagcccc agctaggaga gctagggatt tggcctgtag tttcatgtgg
    28861 ccttgctgga tgcccgtact taccaaggct ttgagggctg caaaattttg ggcaagaccg
    28921 atggacacga taatctgggc taattctctg gcagaaggat ttcctagtag atcatgactg
    28981 agagctacac gtgggttgag gccgatagag ccacccttag tcgctacagg catgggcagg
    29041 gtcatctcac cgaccaattc ttctctttca aggtccagcg tccagcaact aagaccttga
    29101 tagcgtccat ctcgactggc aaaggcatgg gccccagctt cgatggcacg ccagtcatta
    29161 ccagtggcaa tcaaaatcgc atcaatacca ttaaaaattc ctttattatg agtagcagct
    29221 cggtaaggat cagcctgcgc aaactgacta gccaacgcaa ttttctccgc aatctctcgt
    29281 ccttgatcct tttggcggct caagtagcga aaggcgatgc gacagcttgc agtcaccaga
    29341 gaatcggtcg cgtagttgga caggattccc atgagactct gtccctgact gagttcttct
    29401 aagactggtt tcaaggcttc cagcatggtg ttgagcatat tggcacccat ggcttcctgg
    29461 gtatcgacat gaatataaac aacgagaaag tctggttcgc cttttatctg ctcgacatgc
    29521 agatcacgcg ccccacctcc acgtttaacg atagaaggat aggcttgatt ggcaagctcc
    29581 aagagctccg ctttcttgct ggcaatcttc tcttgcgcta gtttaggatt agcaacttga
    29641 taaagggcta cctgcccaat catctgtcgc tgatggactt gtgcagtaaa accacctgca
    29701 cgcttgatga ttttgctggc atagctggcc gccgcaacca cagagggttc ttctgtcaca
    29761 tagggaacgg tgtattcctg accgttgaca agtacctccg gaaccagtga ataaggcaga
    29821 gaaaaagttc ccactacatt ctcactcagc tggtctgcca cagtcacgct catctgttca
    29881 tccttctcca gactagcttg tctctcagga ctaaggagcg cctgagcttt taacagctcg
    29941 aggcgctctt ggtatgattt tttagaaaat ccattccaac ttatcttcat tatttttcaa
    30001 ccttgctata acggcgttgg tggtcgagaa tttcaaccaa ggcaaaatct tgattttcat
    30061 agccagcaaa ctgggcagag tttgtttcat ccaagtttac ttcctcaaaa aagacctttt
    30121 catagtctgc aacggataag acagttcgtt ggttgagctt gttcaagcgg tctttatcca
    30181 aataagcttc atatccttca accaattcac cactgaagaa ctcagccaca gctccacttc
    30241 cgtaactata aagggcgatt ttatccccag ctttcaagct atctgtattt tccaagagag
    30301 acaaaagtcc aaggaaaagt gaacctgtgt agatattccc caccttttga ctgtagagaa
    30361 tagactggtc aaaatgcttt tgtaagaggt cttttttctc ttgaggcagg ctcttatcca
    30421 tgattttttt caagcctttt agcgctaatt taggataagg caagtggaaa caaacagccg
    30481 caaaatcatc caaagtaagc tggtagcgtt tttgatattc aagccaagtc gttttcaaac
    30541 tatccaagta ttgttgggta gaatagacac catttacata aggagttgtc gagtaatttg
    30601 gtcgccagaa atccatgatg tcacgggtct gagctacatt gtcattatta aaggccatca
    30661 tgcgtggatt ttgtgtaatc aacatagcca cacttccggc accctgagtg ggttctcccg
    30721 gagtttcaat accgtatttg gcaatatcac ttgcaatgac caagaccttg gactccggag
    30781 aattttccac atgcaatttg gcataatgga gggcagcagt cgccccatag caggcttctt
    30841 taatctcgaa actacgagca aagggctgga tgcccagcaa gccatgcaca aagacggccg
    30901 cagccttact ctggtcaatt cctgactcgg tcgccacaat gaccatgtca acttcttgtc
    30961 tttcttgctc agttaaaata gagtcactag cactggccgc caaggtcacg atgtcctcag
    31021 ttaggggcgc aatactcaat tccttgagta agagtccttt acttaatttt tcagggtcaa
    31081 ttcccctcgc ttctgctaag tcttgtaatt tcaagacata ttgactggtc gcaaaaccaa
    31141 tcttatcaat accgattgtc atatttacct ctgttttatc attcatgtaa aaaatcgttc
    31201 tatactattt tatcacaaat ggcagtaaaa gagagaaaaa agacttgatt caccaaatca
    31261 agcctcttat tggtcatcat tttaaagaat gattagttgc tagagagttc accgatataa
    31321 gtagctttat aagctccatt cacagttatc agctcctgga ggatcaaatt tcctgagtaa
    31381 gtccttccca tctcatctac aaatttttga taaaactgac tggtcggaat ttctctgaca
    31441 tccttatcaa atgtcttatc aagtgtttta ctaaccttct cagcaatcaa ttgatgctct
    31501 tgccatccac tttgaaactc tgagcccgaa ctagaaacca tgactgggat aaacaacaag
    31561 gtcagtagat ttacagacaa taaggaaagt a
//
LOCUS       DAVBDG010000021        30052 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBDG010000021 DAVBDG010000000
VERSION     DAVBDG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.021"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..83)
                     /locus_tag="VQ526_000931"
     CDS             complement(<1..83)
                     /locus_tag="VQ526_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2554669.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEE"
     gene            complement(91..531)
                     /locus_tag="VQ526_000932"
     CDS             complement(91..531)
                     /locus_tag="VQ526_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2554670.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLNGRGPVWWVPFEEMTAETRQAKPDEIRLPAKQNMV"
     gene            complement(533..1570)
                     /locus_tag="VQ526_000933"
     CDS             complement(533..1570)
                     /locus_tag="VQ526_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2554671.1"
                     /translation="MNQTVEYIKELTAIASPTGLTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            complement(1668..2993)
                     /gene="brnQ"
                     /locus_tag="VQ526_000934"
     CDS             complement(1668..2993)
                     /gene="brnQ"
                     /locus_tag="VQ526_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2554672.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVIGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(3164..3256)
                     /locus_tag="VQ526_000935"
     CDS             complement(3164..3256)
                     /locus_tag="VQ526_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2554673.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(3299..3451)
                     /locus_tag="VQ526_000936"
     CDS             complement(3299..3451)
                     /locus_tag="VQ526_000936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2554674.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(3611..4189)
                     /locus_tag="VQ526_000937"
     CDS             complement(3611..4189)
                     /locus_tag="VQ526_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2554675.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(4243..5643)
                     /gene="pepV"
                     /locus_tag="VQ526_000938"
     CDS             complement(4243..5643)
                     /gene="pepV"
                     /locus_tag="VQ526_000938"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2554676.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(5656..6261)
                     /locus_tag="VQ526_000939"
     CDS             complement(5656..6261)
                     /locus_tag="VQ526_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2554677.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            6432..7232
                     /locus_tag="VQ526_000940"
     CDS             6432..7232
                     /locus_tag="VQ526_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2554678.1"
                     /translation="MKKLSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(7414..8211)
                     /locus_tag="VQ526_000941"
     CDS             complement(7414..8211)
                     /locus_tag="VQ526_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2554679.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLDAETDVAVYGHVHKQLLRYGS
                     QGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKVAYDYEAELELAK
                     SKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDFL"
     gene            complement(8201..10045)
                     /gene="uvrC"
                     /locus_tag="VQ526_000942"
     CDS             complement(8201..10045)
                     /gene="uvrC"
                     /locus_tag="VQ526_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2554680.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(10085..10786)
                     /locus_tag="VQ526_000943"
     CDS             complement(10085..10786)
                     /locus_tag="VQ526_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2554681.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(10865..12097)
                     /gene="murN"
                     /locus_tag="VQ526_000944"
     CDS             complement(10865..12097)
                     /gene="murN"
                     /locus_tag="VQ526_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2554682.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(12102..13322)
                     /gene="murM"
                     /locus_tag="VQ526_000945"
     CDS             complement(12102..13322)
                     /gene="murM"
                     /locus_tag="VQ526_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2554683.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(13434..14213)
                     /locus_tag="VQ526_000946"
     CDS             complement(13434..14213)
                     /locus_tag="VQ526_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2554684.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(14282..15943)
                     /locus_tag="VQ526_000947"
     CDS             complement(14282..15943)
                     /locus_tag="VQ526_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2554685.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLTLLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILHESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(16118..18340)
                     /gene="recJ"
                     /locus_tag="VQ526_000948"
     CDS             complement(16118..18340)
                     /gene="recJ"
                     /locus_tag="VQ526_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2554686.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            18617..19375
                     /locus_tag="VQ526_000949"
     CDS             18617..19375
                     /locus_tag="VQ526_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2554687.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAILSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     LDTTDVDNFFDNPSKPRAQQFLSKIINHESDKVK"
     gene            19386..20180
                     /locus_tag="VQ526_000950"
     CDS             19386..20180
                     /locus_tag="VQ526_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2554688.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDHSILSGYTSKRTALLDDSFKPSD
                     YSIVTKKSNTELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            20193..20870
                     /locus_tag="VQ526_000951"
     CDS             20193..20870
                     /locus_tag="VQ526_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2554689.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            20880..21539
                     /locus_tag="VQ526_000952"
     CDS             20880..21539
                     /locus_tag="VQ526_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2554690.1"
                     /translation="MESILEILTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            21776..22900
                     /locus_tag="VQ526_000953"
     CDS             21776..22900
                     /locus_tag="VQ526_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2554691.1"
                     /translation="MEFSMKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSL
                     TFILATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNL
                     AIYIFASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFL
                     VGSYALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQF
                     AFLKIFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYG
                     HMIIEEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYNFRGDENAVYLDLLRNY
                     AQKNPNFELHLIDSTKDGYLNFEQKKCPNMQPSICVVLFL"
     gene            complement(23298..24179)
                     /locus_tag="VQ526_000954"
     CDS             complement(23298..24179)
                     /locus_tag="VQ526_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2554692.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(24320..25648)
                     /gene="vncS"
                     /locus_tag="VQ526_000955"
     CDS             complement(24320..25648)
                     /gene="vncS"
                     /locus_tag="VQ526_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2554693.1"
                     /translation="MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKNRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(25645..26301)
                     /gene="vncR"
                     /locus_tag="VQ526_000956"
     CDS             complement(25645..26301)
                     /gene="vncR"
                     /locus_tag="VQ526_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2554694.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(26350..26433)
                     /gene="pep27"
                     /locus_tag="VQ526_000957"
     CDS             complement(26350..26433)
                     /gene="pep27"
                     /locus_tag="VQ526_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2554695.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(26614..27993)
                     /gene="vex3"
                     /locus_tag="VQ526_000958"
     CDS             complement(26614..27993)
                     /gene="vex3"
                     /locus_tag="VQ526_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2554696.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(28045..28692)
                     /gene="vex2"
                     /locus_tag="VQ526_000959"
     CDS             complement(28045..28692)
                     /gene="vex2"
                     /locus_tag="VQ526_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2554697.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIQ
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(28705..29982)
                     /locus_tag="VQ526_000960"
     CDS             complement(28705..29982)
                     /locus_tag="VQ526_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554698.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQVSLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         8593 a         6902 c         5588 g         8969 t
ORIGIN      
        1 atctcttcaa aagtggtacg ctgaacacca acaagacgct taaatcgtgc atcagttagt
       61 tgtttacttg cttcatcatt catagaacta ctataccata ttttgtttcg caggaagtct
      121 gatctcatcc ggttttgctt gacgggtttc tgcggtcatt tcttcaaaag ggacccacca
      181 gactggccca cgtccattga gaccgtgacc attcatgtca cctgttcgtc taggaaacag
      241 atgccaatga agatgggcat cgccatttcc tagcagttcg atattcattt tctcagcagc
      301 aaaggccttg gcaactgcct cttggaccac actcatttct tctagaaaac ggagtcttgt
      361 ctcctttttc aaatggtgca attccgtgac atgttccttg gctagaaaga gactatagcc
      421 tgcaaaatac tgatggtctc caatcacaag atagcctgtt tccaactctt tgacaaagta
      481 gggattttct cccttcttga tgagctcaat tctctggcaa ataaggcaca tatcaatcta
      541 ctaaattgct tctaagatag gcatcgacca ttcgttctgt tgcgaccacc gagtcaatat
      601 gagtgcgctc ataagaatga ctagactcga tacccgcacc aagaagggca tgcttgacct
      661 ctgcccctgc agacatagcc gctgaagcat ccgaaccata aaatggatag atgtccagct
      721 taaaaggaat atcttgctct ttagccaagg caaccaagtg ttgacggaag tcatagtgat
      781 aaggacctga agcatccttg acacagatag atactgtgta ctcatctgtc tgctggtcat
      841 ctcccatagc tcccatatcc acagccagat attctactac ctgagcagga atgttagaat
      901 tagcaccatg tcctacttct tcaaaaactg aaaaagcaaa atgagttgtt actggcaatt
      961 caatcttctc tttcttataa atacgaagca ggttgagcaa aatcgctgcg ctgaccttat
     1021 catccaaatg acgagacttg ataaaacctg tatcagtcac aacagttcgt ggatcgaagc
     1081 tgataaaatc accgacctca atcccaagag cacgagtttc tttttcattg cttactttta
     1141 catccaaacg cacttccata ttgtcctgcg tgcgttctgc agttcctgca tccttgtaga
     1201 catggcaaga agtttggtgg atgaggatgg ttcctgatac cttttgacct gtgctagcca
     1261 catgaacggt acagttttca ccttcaatca tgttccaagg aaaaccaccg atacggtcca
     1321 atttaagacg accatctggt ttgacagcac gaacaatagc acccagcgtg tccacatggg
     1381 cagtcacata gcgatgttgc tcatcatttt gacctttgat ggtcacattg acaccaccct
     1441 tggctgtgcg aactggctgg taaccaaaac cttctagagt cttgactaaa taattcgaaa
     1501 tttcacgagt taagcccgtt ggcgatgcga tggctgtcag ttctttgata tattctactg
     1561 tttgattcat ttcttcacct ctttcgctac ctattataac acatttatca tcggataaaa
     1621 aaagaaaatc actcctgtag acttggctac aaaagtgatt taagtgatta ttccatttca
     1681 aaaacatcgc tttcttgctt gtttggtaga accaatgaga gcaagattcc aacaatggct
     1741 ggcaccaacc atgggagaga tgccttggca aaaggaagaa cgctaacaag atttgcaaga
     1801 aactcaacct taaacgagct tcctagtacg cttgcaatgg caataactgt aaccacagca
     1861 attgtcaact gcatacctgg ttttgaaagg gcaacaaatt tgttgacaat gacaatcata
     1921 acgatagcaa tcgtgattgg gtacaagata accagtactg gaattgagta cttgataatc
     1981 gcatcaagac ccaaattggc aatagcaaat ccaatcaagg taaaggctgt cgcataaacc
     2041 ttgtagctga tttgtgggaa gcgctcatta aagaactcag ctgttgacac aatcaaacca
     2101 acagtcgttg tgaagcaggt tacggtaacc atagctgcaa ggaagagttg agctgttgag
     2161 ccaaagattt cttgagtggc ttgtgacaag atgtaaacac ctggtgttcc acccttcatc
     2221 gcttcagctg gtactgggaa atgatttcca agaaaaccta aaccaatgta aagagcgctg
     2281 aaggcaaggg caacaacgat accaataacc caaatagttg aaatgtattc tttcttactt
     2341 gaaaatccaa gttgtttcaa ggtttgaact gcgattacgc taaaggccac tgaggcaagg
     2401 gcgtccaagg tattgtaacc ttctaggaaa cctgtaccaa aggcagaagc ttgataagca
     2461 gctgaagcag cttgaggact tgttccacca tatttgatag ctcccagaac gaccaagata
     2521 acaatcaaaa ttgcaaagac tggcgttaaa atacgtccaa tgcggtctaa gatttttgat
     2581 ggattaagcg aaatcaaata ggctgccgca aaatacagaa ccgtaaatac aatcaagcca
     2641 agtcctttat ttgcatccga caaaaggggg ctaatcccta cttcgtaagc tgttgtagca
     2701 gtacgtgggg tagcaaagaa tggaccgatt gacaagtaaa gaactgagag gtaaagagtc
     2761 gcaaaccaag gcgctatctt cgttgaaatc tcgtagatat atcctttagg atttagcgtt
     2821 ccaataataa gggtcaagac ggcgatacca acgcctgaaa agacaaaacc tgcgatggca
     2881 ggaagaaaat gttctccaga tagagcacct agagaaggcg gaaaaatcaa gttccccgca
     2941 ccaaaaaata ttccaaacag gagcaaacct gttagggtac cttttttagc cataaaaatc
     3001 tccttcatat ttataaaata ctgacctagt ataccacgat taggagtctg aaaaaagcat
     3061 cacgaggcaa atattaaatg ttttcaattt tcttaaaaat aagaattatc taaaaataaa
     3121 actctcctac ccagtccaca ccaagtagga gaaatgcaaa attttaaagt tcttcaaagg
     3181 ctaacatgcc accctgaaca ttgataacat tataaccttg ttctaacagg aattggcaag
     3241 cacgcgccga tctcattcca gatttgcaaa taatataatg caagcgatct ttgtccaatt
     3301 aatcataact atcagccaat tgactaagcg gtaggttgtg ggcacattct aaatgaagag
     3361 ctgcaaactc atccacttct ctcacatcca ctagagaaag ctggtcgttt tggtaaagct
     3421 ggtaaaatgc gtcaaaggct atttctttca ttctttttct tctaatactc ttcgaaaatc
     3481 tcttcaaacc acgtcagcgt cgccttaccg tgcgtatgtt accgacttcg tcagttttat
     3541 ctgcaacctc aaagctgtac tttgagcagc ctacgactag cttcctagtt tgctctttga
     3601 ttttcattga ctataaaatg gttttaattc tttttttcaa atctggcact acttctgcct
     3661 caaaccaagg atttttggcc atccagattt gatttcgtgg tgatgggtga actagcggaa
     3721 aataggctgg cagatagtct ttatagtgtt tcaccctctc cgttaccttc ccactgattt
     3781 tctcctgtaa atagtaggct tgggcatatt gcccaatcaa gagggttaac tgaatatcag
     3841 gcaattcctg taagagctgc ggatgccatt tttctgcaaa acctgtacga ggcggaagat
     3901 cacccgactt gccatgtcct ggaaagtaga aatccatagg caaaacagca aaataacctg
     3961 aattgtaaaa ggtatcttca tccacaccta gccagtcccg caagcggtca ccacttttat
     4021 ctttccagta aaggcctgct tcttgagttt taagtcccgg agcctgaccg atgatattga
     4081 tgcgagcagt ttttggcgct gcaaagagag gctcaatgcc acgctctgta tagctggcat
     4141 tctgtgaatc cgccatgata gcctgtttga ttctttcgat ttgagacatc tactttccct
     4201 ccaaaaagaa gtccaagcat aagatctcag acttctatcg ttttatttga tcaattcgta
     4261 aatagcttcg gcataaattg ctgctgctcg gaaaagatca tccaaggcga taaattcatt
     4321 ggcttggtgc atggtatcaa tcgagtctgg gaacatagca ccgtaggcaa ctccgcgttc
     4381 tagcaagcga ccaaaggttc caccaccgat gacttgttca tgacctttaa ggccagtttg
     4441 tttttcatag atattcaaca aggtttgcac aagtggatct tccattggca catagtgagg
     4501 cgtgtgaccg tgttcagaca ggctaacaga aacaactggc aagttttcaa ggattgactt
     4561 gatttgttct ggacttgttc cttttggata gcggatgttg agggcaatgg tattatcagc
     4621 acttgtttca tcgaagcgga agacgccggc attcatagaa agagcaccca tcttttcatc
     4681 cacatgagca atcttaagat tttcaccctc atgatcgttc aagagaattt tacctgcgat
     4741 gtcaaggtag tctttggctg gaccagcaaa gccaaactgg ctgaggaaga gggcaaggta
     4801 agttgcgcca ttgacacctg aagcaggcat agcaccgtgg gctgatttac caatgatcgt
     4861 caccttgtat ttgccagctt cttcttggag ttctcctcta agtttgtgtt ctgcaacaaa
     4921 ggcatctagt ttagcttgca agtcagccaa atcacctgaa acgactgctg ttgctgattc
     4981 tggtaccata ttttcacgta aaccacctgt aaagctgtga agacgggcag cacctgtatt
     5041 ttctcctgca aagtggaggt attccgtgat atttcctttt tcaccattga tgattggaaa
     5101 ttcagcatct ggagagaaac cgaaatctgg tttggcaagt cctacgtgct caaagtagta
     5161 gtccatgtct gcccagcctg attcttcgtc tgttccaacg atgaagcgaa ctttcttaga
     5221 agttggaaga cccaattctt tgatgatttt caaaccatag taacaagctg ttgtaggacc
     5281 cttatcgtcc gaagccccgc gcgcataaag gcgaccatct ttgatagttg gtgtgtaagg
     5341 atctgtgtcc caaccgctac cagcaggcac cacatccata tgggcaaaga ttccgagaac
     5401 ttcttctcca tcaccaaact caaaatgtcc tgcgtagtta tcgacattct tagttgggta
     5461 gccatcgcgg tctgcgattt caaggaattt ctccaaggct tttactggac caggtccaaa
     5521 tggatgctgg gcatcagcct tgctatcatc acgttctgag ttgatttcca aaaggctaaa
     5581 caagtcagcc aagaggtctt ctttgcgttt ttctacttct gctgtaaaat caattgctgt
     5641 cattttttct tctttctatc ttttctcgat gatttcatct actggcaagc ggtagcttgg
     5701 ttccaatttg tcgtctgtgt aacccactgt gatcaagagt tctgggcgga aacggtcttc
     5761 gatttccaaa acttcattaa cttttgattt gtcaaaacca aggataatat tggaaccaat
     5821 tccttggtct gtaagagcga gaaccaagtt catagcaacc aaacctgcat tgagtgctag
     5881 gtagtcgctg acttgttgtt cattgtagcg tgcaaattca gctggcaaat ttttcatgaa
     5941 gtattgaagt tgttcttctg agaaattgtt agcaccacca actcgggcaa tcttacgagc
     6001 gcgtttggca aggtctgtat ctgtaaatag ggcaatggtt acaggtgctg atgatacctg
     6061 ctcaaagttt gaaccataag ccaactttgc cagttcagca tttttctcac gaacaactac
     6121 aaatttccaa ggctggctgt tgtgggcgct tggcgccaag gttgcaattt cgatagccgt
     6181 acgcacatct tttggatcaa caggcttatc agtgaaatgc ttggtcgcat gacgtttttt
     6241 atttaattca agaaatttca taatcgattt ccttttctaa ttctactgct tccattctac
     6301 cataatttga aagagcttgc agggattttt catgattaag tttttctatc acagacataa
     6361 aagatatata ttggtttatc aggaaagttt ctgataaact agcaagtact ttacatttga
     6421 atggagacag tatgaaaaaa cttagcctat tactagctat cctaccattt ttggttgcct
     6481 gtgggaatca agctacaccc aaagagacta gcgctcaaaa gacaatcgtc cttgctacag
     6541 ctggcgacgt gccaccattt gactacgaag acaagggcaa tctgacaggc ttcgatatcg
     6601 aagttttaaa ggcagtagat gaaaaactca gcgactacga gattcaattc caaagaaccg
     6661 cctgggagag catcttccca ggacttgatt ctggtcacta tcaggctgcg gccaataact
     6721 tgagttacac aaaagagcgt gctgaaaaat acctttactc gcttccaatt tccaacaatc
     6781 ccctcgtcct tgtcagcaac aagaaaaatc ctttgacttc tcttgaccag atcgctggta
     6841 aaacaacaca agaggatacc ggaacttcta acgctcaatt catcaataac tggaatcaga
     6901 aacacactga taatcccgct acaattaatt tttctggtga ggatattggt aaacgaatcc
     6961 tagaccttgc taacggagag tttgatttcc tagtttttga caaggtatcc gttcaaaaga
     7021 ttatcaagga ccgtggttta gacctctcag tcgttgattt accttctgca gatagcccca
     7081 gcaattatat cattttctca agcgaccaaa aagagtttaa agagcaattt gataaagcgc
     7141 tcaaagaact ctatcaagac ggaacccttg aaaaactcag caatacctat ctaggtggtt
     7201 cttacctccc agatcaatct cagttacaat aacactcttc gaaaatctct tcaaaccacg
     7261 tcagctttat ctgcaacctc aaagctgtgc tttgagcaac ctacggctag cttcctagtt
     7321 tgctctttga ttttcattga gtataagata tactaaaagc gattggacca gccaatcgct
     7381 tttttagttt gcattggcta ttttaggaaa ctcttacaaa aaatcaaaaa aattcttcac
     7441 atcctctaca tacccatgct tttctattaa gctggctaat aattccagat tgtgcccctg
     7501 atagttatcg tcacgacgca gttcttcata catttcgata aagggaagcc ccttggactt
     7561 ggccaattct aactcagctt cgtaatcata agcaacttta cgaaattgga tattgagtaa
     7621 ttccccatct tcaacttcta tcacggcata ctgggcacgg tgatttttta acgcctccca
     7681 attaaaatag ggcatgccaa tcgaccctgg attgatgatt tgttgccctt gacttccata
     7741 acgaagcaac tgcttgtgaa catgaccata aactgccacg tccgtttccg catctagcaa
     7801 gtcaccacca tagtttttgt caggtaaatt atgagagata gaaaagcgca atccgtcaat
     7861 ttctttcttt tccagcaaag gcaagcttcg tagccagaca atcgttgcag gatccattcg
     7921 ctccatcaaa tactgtgtca tacgcaagag ctgaacttcc tgtgggtctt ctaagccata
     7981 ttgcccatct aaagcctcaa ggacacgatc atcccaattg cctcgaacac ttgctgtgat
     8041 aggaaggtcc tttagcaggg cgactaagtc atttgcgcct ggaccaggaa gaaaaatatc
     8101 tcccagaagc caatattcac tggccccttg atttttagca tctgcaatca ctgcttctag
     8161 cgccgtcgca ttgccatgaa catctgataa aattgcgatt ttatggttca ttgtggtttc
     8221 cttccgtttg gtaatctact ctttcttctg ctacttgagg caaggcttct ccctgcgggt
     8281 tcaactttct ttgcacagcc tctgcaacga ctctaggtac cccaacttcg acaatctcat
     8341 ccacactggc ttccttgatt ttggtcaaag acttgaaatg cctcatgaga ttttgcttgc
     8401 gtttaggtcc cagaccatca atcccatcca attgagatga gaaagaattt ttggagcgca
     8461 gttggcggtg gaaagtgata gcaaagcggt gcacctcatc ttggatgcgt tggaggagga
     8521 aaaattcctg agaattgcga gacaagtcca ccacctcaag cggatctcca aagagcaatt
     8581 catgggtttg gtgcttatca ttcttttgca gcccagcaat tggaatatcc aagcccagtt
     8641 cctcttggat aacctgctta gcgatattga cttgaccttg ccccccatca atcacaatca
     8701 aatctggagg agtcaaagcc tcacgctgta ctcgaccata gcgtctgcga atgacctctc
     8761 tcatgctggc atagtcgtct ggtccaacaa ccgtttttat cttgtactta cggtaatcct
     8821 tcttactcgg tttaccgttg acaaagacca ccatagccga aacagggcta gttcccatga
     8881 tattagagtt atcgaaggac tcgatacgta ctggggtcgg gatttggagc aaacgcccta
     8941 gattttcaat agctccttga gtcttttcga cagatttttc tagcagattg aacttctgct
     9001 ctagactaac acgagcattt tttatggcta gattgaccag ttgttttttc tctccacgtt
     9061 gaggcttaag aatcttggaa tccaccaagg ccttgacagc ctcttcgtca atatcctgcg
     9121 gaatcagtac ctcattggga actagatgag atttttcttg atagaattgt cctacatagg
     9181 tcaaaaaatc ctcatctgga tcattgaagt aggggaagag attgacatcg cgctcgataa
     9241 gcttaccctg acggacaaag aaaacctgca cacacatcca gcccttatcc acatagtagc
     9301 caaagacatc gcgattttgc aaatctttcg ccatgacccg ttgcttggtt cgaagcgttc
     9361 caatagcctg aatcaggtca cggtattccg ccgcacgttc aaactccata ctttgtgctg
     9421 ctactgccat tttactcttg agatcatcga tgattttgtc atcctgacct ttcagaaaat
     9481 cagacacctc ctgagccata gacttgaaat aagcctcatc cttcttacag atggtgtggg
     9541 ccatacactg gccgatatgg taataaaaac agaccttaga gggcgggttg gtacattttc
     9601 gaaaagggaa aatcctatct agaagtcgct tgatttcatt ggctgccccc acatctggat
     9661 aagggccaaa ataaagacca ccgtcctttt tgacctgacg agtaatgata agccgtggat
     9721 agcgctcatt ggtgattttg atgaaaggat aggacttgtc atccttgagc atgatattgt
     9781 acttgggctt gttttccttg atcaggttga tttctaggag aagtgcctca atattagact
     9841 ccgtaacaat aaattcaaaa tccacaattt cagacaccag agcctctgtc ttggtatcat
     9901 gacttccacg aaaataggac cgtactcgat tacgcagatt tttagccttt cctacataga
     9961 taatggtgcc atttttatcc ttatgaatgt agcaaccagg gctggtcggc aagagctcta
    10021 gttttgattt aatcaagttg ttcatagttc cattatagca aaaaaagtag ggaaagatag
    10081 taccctactc actagtctca tatatttttc ggaggtttgc aacatcctct tcctggatac
    10141 cataaaagga acctgctggt tgcatgacag acttctcatc tgtatggtcc aagccaatcg
    10201 catgacctaa ctcatgttct gccgtatgga caaggcgttc ataggagtag ccatagtatg
    10261 gattggacaa ataataatga ttcaaccgca ccgttacgga caagaattgc cctgttaaga
    10321 gattagtttg actttccgcc tctcctgcca caggagtgcc tccgtcgttc atctccgtag
    10381 ccgtaatatc cgccttacta gactcagtca cgagttcaaa gttaaaagca ccagtttgat
    10441 tccagttctg gattgcttct aaataagcct cttgaaagcg tgaatccatc tgcggatcca
    10501 ggtaaatacg agcagaggcc tgtgcccacc ttccttcaga atgctggtga ctatctgtct
    10561 ggcttaactt aggcagttgc cctgttttct cccattggtc aatactttgt tgaccaattt
    10621 ttactgaccg ttctgcttgc tttagcgctc cttgaaaatc cccgttcaga taccagagaa
    10681 gtccgaaagc aagcacacat aagagcacaa ctatccaaac cagacgccaa aacaaacgcc
    10741 acacaaaaga aaagaaagcc cctatcaaac gaaaaagcca gcgcatgtct ctccaccttt
    10801 ctagcaaata agaattattt tactaaaaga agctaaaaga aatctaaata ctgacttttt
    10861 catcttaacg tcctactatt tttttgagta actggatagc tttgtatttt aaaggcgatg
    10921 gatggtaacg gaaagtaccc gctttgcgta caatatagcc attaaaattc tgtttaaaac
    10981 gcaaaacacc atcacttcca tcaaaaatcc cttgaatgcc taggaagttg tatttaggta
    11041 ttccacgttt tatgctttcc aacataacat atttttgaag cagggcaggg gcatagaact
    11101 tattaaactc agtgtaggaa ccactaaaga gataagtcgt ttcctgaggc atataaacaa
    11161 ataaactccc agctaaaaca atatcttctt ctccatattt ttcaatcaag tctcgcgctt
    11221 ctgcttttcg aacttcaaac gtttcaaatt gactagaata ttctctcagt tgattttgtt
    11281 ttttctcaga atgaggattt ttactcaaat caagtcgcaa cttgtccaag ttttcttcta
    11341 gtttactttg ttcaccttgc aatttgctca tatagtccga aaaatttaag cttgctatga
    11401 gaaactccgc ttgttctcca aaagtatcat aaaaatgctc ataatattct aaacttttat
    11461 cactatattc tctacgttca gaggtttctt ttgttatatt cttaaaaatc gatagttctt
    11521 cacgttttaa ctttttcaac cgaatgccaa aggtttcagc ctttttcacc aagggtttac
    11581 cctttttgct aaaactttta agcaaactct tttcagttaa ttcagttaaa tctttatagt
    11641 ataaccaatc tggttctcca cctgggtaac ctgttgttaa gccatcaaat tgataaccta
    11701 aatcagtcaa accttgaata atactttttt tctcagcatc tattggatta ccttggctat
    11761 caaaagtttg ataagtttca taaggtttta caagcaactc taatacacca ttttgcttgg
    11821 catattcttt taactctgca taaaaaactg gaagagcatc ttgttgggta taaatcggcc
    11881 ccgaattgag ttccatatgc agaccaccca gcatgggcaa gctataaacc agagctgcaa
    11941 cttgaatttc tccttcttgt ttcaaagcaa gataaacaat tcgagcccct cttttttcta
    12001 gcaaatcccc catctggaca gattgcataa aggaacgaga agaaacctga tcagaataag
    12061 tctgaaactc ttctttcgtg agtgttgtta gtgccatata cttactttct atgttttttt
    12121 cttaatgttt tacggaaatc aagagcaagt cttaacagag gatagagagg atgagtgggc
    12181 attgtaaatt cacccaagta ttcttcaatc gttggattaa atttttcctt aaaatgataa
    12241 agtccaccat tgagagagtt ttcaacacca cctaaatttt gccaaaccat acctcgctca
    12301 aaggcatagc gagccgtttc ataccatgtt aaaattggtg cattgtaacg tttaaaatca
    12361 tcatccatac cagcatatat attgacagag gtagtaccaa attccaaact caaagtagcc
    12421 gctaaaggaa ctctcgcttg acctacatct atatattcct gcaagaaggt caattcctct
    12481 aacaaacgtt ctttttcctt cttctgcgct tctacttttg aaattcgagt cgactcagta
    12541 aaggtctctt ccaaggctct attttttgct aactgttctt ctaattcttg cgaacgttta
    12601 gaaacatcca aggtggccaa ggtgatatag gccttgtcct taaaattatc taacaatttt
    12661 ttataatagg cttcattcct caaatgaatc tcttttcgct tctcagtttt tttcatcaac
    12721 tccgaaaatg aatctaatag ttccagtcca ccatattgaa tctcaagccc tttgtttcgt
    12781 gctgttcgaa tagcctgttt tgttgacttg gaaagtttat cttcttcaaa attttccttg
    12841 tatattttcg cctgaatacg aggttgaatg gtgtctccca tttcctccgt ttttcctgac
    12901 caccttactc ccatttgttg caaactatca ataatagcca gattttcagg aaattctgtc
    12961 ttttcctgat tgattaaact ttgagatagg caaatacttg ggtcaaaagt cacaaaaacc
    13021 gctctcttac tgcgagcata ggacttaata gactgaatga caaaattcaa gagttcttta
    13081 tccccataat ccaatatagg tcctcttggg atgtaaaaca ttttatagcc tagcggaaga
    13141 gttctaatca aaatactagc tgtcgccagt aatttttctt ccctgtaaac accaaacttc
    13201 tcatgttgcc aattagactt aacttcctcc caagcactac tttgtaatac attggctaat
    13261 tcatgttctt tgacaaactg atcatattct aatgtgggaa tgccaatttg ataacggtac
    13321 atttcctact ctctttcctc cagtatttta ttactattct actactttcg tttgaaaagt
    13381 ctagtgaaaa catattttat tttagttcta ctccccctat gctgaagttc aaactaagtc
    13441 agtctctctt ctaatttgaa atcaattggt agggttgtta aaaaaacttc caattgtttt
    13501 tcccagtagt accactcgtg agttccagcg ctatggctat aggtcacatc aaaacctagt
    13561 tttttgagat ttttcactgc gagattattg gcttcgtaca agaaatcctg ttcgccacac
    13621 cacgcccaaa gtttggtctt tttatccgat tttttagcca gactttcaag agaataggga
    13681 ctagttgtcc agtctttaat ctctccaaaa acacctctcc agtaggctgg acttcccaga
    13741 ttttgacttt caggagaaaa gttttgaaaa ctgagggcac ctgaaaagct agctgcatga
    13801 gaaaaacgat ttgtcgcaag agccagtttg aagcagccgt agcctcccat agaaagacca
    13861 gcgataaagg tcttttcacg cttgctagtc atattaggga agaagcgttt cagaacctgt
    13921 ggcaattcct ctgctagagc cgtgtagtag tcaaaaccat actgggtatc ggtgtaccaa
    13981 ccattgctgg tattgggcat aacaacgatg agattagttc ctcgaagcaa gcgttctaca
    14041 ttggtccgct taagccaact attatgattt ccagacatcc cgtgcaaaag gtacaagacg
    14101 ggaatatctt cacactctgg ttcttccact cgattggcat cagggtagag gacattcacc
    14161 ccccactcca tatccaatac ttgtgagtaa tactcgattt tcattactgc catgattttc
    14221 tccttctatt tttctataag aaaaagccga aactcgactt tctctctgtt tatttcaacg
    14281 attattttgc ttccatgact actggtaaaa tagctggacg acgcttggtt tggtcaaaga
    14341 gatacttggt caaattatct cgaaccttcc ctttaagatc tgcccagtca aagtcatctc
    14401 cttgaagata ctcttctacc gtttggttaa tcaattctga actttcatgg agaatatcgc
    14461 gactcttctt gagataaaca aatccgcgcg tatgaacacg agccctagcc acaattttct
    14521 tctcacgacg gttgactgta atagccacga tgaaaattcc atcctctgac aagaccttac
    14581 ggtcacgaag aacaacattt ccaacatcac caatggcatt cccatcaatc aagatatctc
    14641 ctgctgaaac cgatccagct ggaacaaagt ctccattctc gtaagccatg gtcgtcccct
    14701 ttttaggaat gaagatgtgt tctggcaaca tcccaactgc catggcagcc ttagcgtgag
    14761 catccaactc acgatactcc ccttggacag ggaagaggta cttaggttgc aaaagattga
    14821 tcatcagctg caaatcacgc acatttccgt gccctgatac atgtaaactt tgggtaatca
    14881 atttgacaac cccacctgcc tgataaatca tattttccac acgcgcaaca aaggcttctt
    14941 tagcaataga cggagccgta gcaatataga ccaggtcccc atccttgatt tctacataac
    15001 gatggcgacc aatcgacatc ttacgaagtc cattgatagg ttcacccata cgacctgtct
    15061 caagaataat caactcatgg tcttcaaagc gagacatatc tttaggctta atcaagagaa
    15121 tttcgttggc taaagacaac ttcttaagac gaatcgctgt gcggacgata ttttcaatat
    15181 caaatcctgt caagacgata cgtcgacctg ttttatccgc agcgtcaaaa atctgctgaa
    15241 tacgagaaag attactggaa acagctgcaa cgatgatacg accttcccag tcagcaatag
    15301 tttgggtaat ttcatcccta acttcacttt cactagccac ctgaatattg ctgtctgcat
    15361 tggccgaatc actgaggaga gtcaggacgc cgtcacgacc aatctctgcc aaacgagcaa
    15421 aatcagttgc ataagattca ctagccgttt ggtcaaattt gaaatcacct gtataaacga
    15481 tgcttccttc cgatgtcttc aagacaattc ccagactctc tggaacggag taagtcgtag
    15541 ggaagaagga aaccactgtc ccaccaaaat caatctccgt attctcatca atgacatgga
    15601 aatcattaaa tttcttaacg gcatcatttc ctttgacaaa gagctttgcc aactcaatgg
    15661 tcaactcaga cccaaataca ggaactttag cctctgccaa gagataaggt agagcaccaa
    15721 tggcatccgc atgcccgtgg gtcaagaaaa ccccagcaat acggtcgcta ttttcaaaaa
    15781 ggtaatccat gtttggaatc accacatcga cccctaattg ttcattttca ggatatttta
    15841 accctacatt caaaacaaaa atggactctc caatttcagc aatgtacata ttttttccat
    15901 tctcacgcac accaccaagt gttgttaaac tgatattact catttttcct ccaaaatctt
    15961 agtttatctt gattataggg caactctacc ctcttattct aaaagttcta atactttcag
    16021 ccgaatcttt tcctaccatt ataccatttt tttgatgatt ttcaaaatga aggtctgttt
    16081 tcaaaaaaga gttgatttcc caactctttc ctaacttcta ctctttttcc atcaaaaaat
    16141 cataaatttc ttgcaccgta cccagcgcca tcatttcttg gtctttaacg gtttgtttga
    16201 gattttggta aatttgactt tctcctatct cccgctttgg cgcctcttta ttgactgtca
    16261 tcacaccatc ttttatcgtc acaaagccta gttcttcaaa tacttgaatc atcttgacca
    16321 gcaagatttg ttgaatatta agatatgcag ccaaatcttt cagcttgtag cgaatatcaa
    16381 actctgggaa ctggtaaata gtcttgtaca atttggcaaa ctgatctcta gtcccataac
    16441 ctgtcagata ataggccttg tcaatatcat ttttgaaata gacagcagag aaatgctgtt
    16501 cctgaaaaat ggtcttcagc tgagtaatat cctctggaat gttttttacg acaatagctt
    16561 cactagccgc aagatttggc agttctccag gaaaatccaa gactggaaca ccttctggca
    16621 agactgcatt ttttccacga atgttaaaaa gttgaacacc ttccactcgc gcatccacca
    16681 tcatcaactg gagggcagtt tggccattcc attggttgac agacaattta accgctaact
    16741 ctagattctt ggtttgagaa aactctgtcg cccatctgcc ttgaccaaag gctaccactt
    16801 caaaactcgc ctcacccttg gaaattttca gctttagatg ggcattacct gcccccatag
    16861 tacgagcact ttcgacctga aaattcttga tataaaaaat aggtttctga ttatccattc
    16921 caaaaggagc taaacgttca aaacttttga ccgtttccaa gctaagtgcc tccaaatcca
    16981 actcttcatc taggtttaac ttattcttgc caccagcatc tgcacctttt tcacgaacat
    17041 aatcttccaa aacctgagat aaatctgaga gttgctcaac ttccagcgtc atacccgctg
    17101 cacctgcatg acctccaaag gcgatgaaga ggtctcgatg gggatccaga gcttcaaaaa
    17161 tatcgaccgc ttccacacta cgagcactgc ccttggcacg accgtcttct atattaagaa
    17221 caatgactgt ctgtcccaat tcttccaata aacgaccagc cacgattcct agaaccccag
    17281 gattccagcc ttccttggcc aagacctgaa ccttcttctc aggatccacc atggtcttgg
    17341 cttcttcata gatagactga acgatttcct tgcgctcttc gtttttctgg tgaatcataa
    17401 gggcaatctc atgcgcttcc tcatcatcaa atccagtcaa caaatcaatg gcaggattgg
    17461 gatcatccaa gcgacccaag gcattcaaac gaggagcaat ctggaaacca accgtttctt
    17521 ctgttacttc gttggcagca atcccagcca tgtccagcat ttcttgcaga ccaatgcgct
    17581 gggtatgacc caacatttcc agaccatatt gaactaagat acgattttca tccgtcagac
    17641 tcaccatatc tgcaatagtt ccaatagcga ccaaatcaag caattccact tgcacttctt
    17701 ctaacagggc acaagccaac ttgaaagcaa ctccacaacc agccaaatat ttaaaaggat
    17761 aatccgcatc tggatgttca ggatggacaa tagcataagc atctggcagg gtttcaggca
    17821 tggaatgatg gtctgtcaca atgacatcta ctcccataga ctgagccaat gcaatagcct
    17881 catgaccagc aaccccattg tccaccgtca caatcaagga aattccttct tgctcgataa
    17941 agtatttata aacactagca ttagggccat agccatcggt aaaacgattt ggcaggtaaa
    18001 ctcggcactc cgcaccaagt tgttccaaac tttccttcac aatagaagcc gaagtcatgc
    18061 catccgcatc atagtctcca taaacgagaa tattttcccc ttcttcaata gcctgacgaa
    18121 tccgctccac tgccttgtcc atatcatgga gcagataagc atcatgtaag tcctccaagg
    18181 aaggttctaa aaacttcttc agactttctt ggtcctgaat ccctctctca aacaataacc
    18241 gagccacctc aggacccagt ccagccttct tggctatctt tgtaaaatcc gcatcttcta
    18301 cctgcagggc aaactgccat tcataagtag gtgttatcaa aaagacatcc actctttcta
    18361 agaattctat tgcttcatta taacatgatt tagacgtttt ctctatccag attgcttttt
    18421 tataacattt tatagtatat tgaaactaga atagtacacc tctacttata aaatattgtt
    18481 agaaatcgat ttgactgtcc cgatcgattt gtccttttct tatttcacta tagtgaattt
    18541 tttcagatac ggtttgacaa gaacattctt ttattgtaga attgtgttat aaaatctaac
    18601 ataaaggaga tctctcatgg ctttagtaga atttaaaaac gtcgaaaaat attacggaga
    18661 ctaccacgca ctccgcaaca tcaatctccg ttttgaaaaa ggacaagttg ttgtcctgct
    18721 tggaccttct ggctctggga agtccactct tatccgtacg atcaatggtt tagaggctgt
    18781 tgacaaagga agtctcctag tcaatgggca ccaagttgct ggtgccagcc agaaagattt
    18841 ggtacctctt cgcaaggaag tcggcatggt ttttcaacat tttaaccttt atccacacaa
    18901 aacggtgtta gaaaacgtga cacttgcgcc cattaaagtt ctaggaattg ataaaaaaga
    18961 agctgaaaaa accgcccaaa aatatctgga atttgtaaat atgtgggaca agaaagattc
    19021 ctatcccgcc atactatctg gtggacaaaa acagcggatc gccatcgctc gtggtcttgc
    19081 tatgcatccg gaactcctcc tctttgatga accaacatct gctcttgatc ctgagactat
    19141 cggagatgtt ctagcagtta tgcagaaact ggcgcatgat gggatgaaca tgatcatcgt
    19201 tacccacgaa atgggctttg ctcgagaggt tgcggaccgc attatcttta tggccgacgg
    19261 agaagtttta ttagatacga cagatgtcga taactttttt gacaatccaa gcaaacctcg
    19321 tgcccaacaa ttcctcagca aaattatcaa ccacgaaagt gacaaagtca aataaggagg
    19381 cgcctatgaa aaaaaaattc tttttatcag cattattgat tagccttttc ggccttgctg
    19441 ctgccaaacc agtccaggct gatactagta tcgcagacat tcaaaaaaga ggcgaactgg
    19501 ttgtcggtgt caaacaagac gttcccaatt ttggttacaa agatcccaag accggtactt
    19561 attctggtat cgaaaccgac ttggccaaga tggtagctga tgaactcaag gtcaagattc
    19621 gctatgtgcc ggttacagca caaacccgcg gcccccttct agacaatgaa caggtcgata
    19681 tggatatcgc gacctttacc atcacggacg aacgcaaaaa actctacaac tttaccagtc
    19741 cctactacac agacgcttct ggatttttgg tcaataaatc tgccaaaatc aaaaagattg
    19801 aggacctaaa cggcaaaacc atcggagtcg cccaaggttc tatcacccaa cgcctgatta
    19861 ctgaactggg taaaaagaaa ggtctgaagt ttaaattcgt cgaacttggt tcctacccag
    19921 aattgattac ttccctgcac gctcatcgta tcgatgcctt ttccgttgac cactctattc
    19981 tatctggcta cactagtaaa cggacagcac tactagatga tagtttcaag ccatctgact
    20041 acagtattgt taccaagaaa tcaaatacag agctcaacga ctatcttgat aacttggtta
    20101 ctaaatggag caaggatggt agtttgcaga aactttatga ccgttacaag ctcaaaccat
    20161 ctagtcatac cgcagattaa ggaggatacc ctatgacaga tttatcatct tggacagcct
    20221 attttcagga ttttggacaa tttttcaatg gtttcctctt tactcttgcc ctagcgattg
    20281 gatcctttat cctcgccatg gtcttaggaa tcttctttgg tgccatgtca accagtaaac
    20341 gtccaatttt acgcatttta gctcgcatct tcgtggaatt ttaccaaaac actcccctct
    20401 tggtgcagtt tgttattgtt ttttatggcc tacctcttat cagtgaccac atcatcatga
    20461 ttccaattta ttggacagcc gttctctgcg tgggactcta tcacggcgct tatatcgctg
    20521 aggttattcg ttcagggatt cagtctattc ctagtggtca gatggaggcc gccttgtcgc
    20581 aaggttttac ctatatcagt gccatgcgct tgattatctt gcctcaggcc ttccgcatca
    20641 ttctccctcc attgaccaac caaattgtta acctcatcaa gaacacatct actgtagcta
    20701 tcatctctgg agtagacttg atgtttgtga ctaagtcttg gtcggctctc aacggaaact
    20761 atatcccagc ctttttaggt gctgctcttc tctactttgc cctatgcttc cctgttgccc
    20821 agtttggtcg caagatggag caagccaata aaaaagccta ttcactttag gaggttacta
    20881 tggaatctat tttagaaatt ttaaccccag ataacctagt ctttatcttt aaaggatttg
    20941 gcttgaccct ctatatttct ctgattgcca tcatcctctc tactatcatc ggtaccgtgc
    21001 ttgccgtcat gcgaaatgga aaaaatccta tcttacgcat tatttccagt atttatatcg
    21061 agtttgtgcg caacgttccc aaccttctct ggatttttac tatctttttg gtgttcaaaa
    21121 tgaaatccac accagcaggt attacagcct ttactctctt tacatcagca gccttggctg
    21181 agattattcg aggcggtctc aatgccgtag acaagggaca atacgaagca ggaatgtcac
    21241 aaggcttcac ctcagcccaa atcctctact acatcattct cccacaagcc atccgcaaaa
    21301 tgctaccagc catcatttct cagtttgtta ccgtgattaa ggataccagt ctcctctact
    21361 ctgttatcgc cctacaagaa ctctttggag ccagccaaat tctcatgggc cgttatttcg
    21421 aaccagagca ggtcttcagt ctttacatcc tgattgccct catctacttc agctttaacc
    21481 tagcgatttc tagcctgtct catatgctag ccaaacgttg gcaacaagct gcagaataat
    21541 actcttcgaa aatctcttca aaccacgtca gcttcacctt gccgtactca agtacagcct
    21601 gcggctagct tcctagtttg ctctttgatt ttcattgagt ataaacagcc actctccagt
    21661 tcaattggag agttttttga tgagagtaga tatttataga cctcaatttg acaccataca
    21721 ttttctatga taaaatgtaa caaattcatt tacaacaaat cgaaatctaa atgaaatgga
    21781 attttctatg aaatcagtca aaggactact ctttatcata gctagtttta tcttgactct
    21841 tttgacttgg atgaacactt ctccccaatt catgattcca ggactagctt taacaagcct
    21901 atctctgact tttatcctag ccactcgtct cccactacta gaaagctggt ttcacggttt
    21961 ggagaaggtc tacaccgtcc acaaattcac agcctttctc tccatcatcc tactaatctt
    22021 tcataacttt agtatgggcg gtttgtgggg ctctcgctta gctgctcagt ttggcaatct
    22081 tgccatctat atctttgcca gcatcatcct tgtcgcctat ttaggcaaat acatccaata
    22141 cgaagcttgg cgatggattc accgcctggt ttacctagcc tatattttag gactctttca
    22201 catctacatg ataatgggca atcgtctcct tacatttaat cttctaagtt ttcttgttgg
    22261 tagctatgcc cttttaggct tactagctgg tttttatatc atttttctat atcaaaagat
    22321 ttccttcccc tatctaggaa aaattaccca tctcaaacgc ttaaatcacg atactagaga
    22381 aattcaaatc catcttagca gacctttcaa ctatcaatca ggacaatttg cctttctaaa
    22441 gattttccaa gaaggctttg aaagtgctcc gcatcccttt tctatctcag gaggtcatgg
    22501 tcaaactctt tactttactg ttaaaaattc aggcgaccat accaataata tctatgataa
    22561 tcttcaagcc ggcagcaaag taaccctaga cagagcttac ggacacatga tcatagaaga
    22621 aggacgagaa aatcaggttt ggattgctgg aggtattggg atcaccccct tcatctctta
    22681 catccgtgaa catcctattt tagataaaca ggttcacttc tactataact tccgtggaga
    22741 tgaaaatgca gtctacctag atttactccg taactatgct cagaaaaatc ctaattttga
    22801 actccatcta atcgacagta cgaaagacgg ctatcttaat tttgaacaaa aaaagtgccc
    22861 gaacatgcaa ccgtctatat gtgtggtcct atttctatga tgaaggcact tgccaaacag
    22921 attaagaaac aaaatccaaa aacagagctt atttacgaag gattcaagtt caaataagta
    22981 atcatactct cctgggattt cccaggagag ttttttatag ccaaatgaaa taagacctga
    23041 acgatggaat tttggcattc aaatcaattg ataagaatat tttatagtgg agtgaaatag
    23101 gatacgaaca aattgattag gaaaatcaaa tgaatttata gaaatctttt agcagttatg
    23161 ttgtcctatt ctagtttcga aacgctatag aagcagcatt gtgctagtct agattcagtt
    23221 tactatacta aaacgagtag cttgaaatca aaaaacccac cctcacaggc aggttttatc
    23281 tgtattattc agctagatta tgctttacct tctgaaccga atacgtcgat acgttcttca
    23341 accgatgctt ggatagcttt tacaccgtca gccaagaatt tacgtgggtc gaagagtttt
    23401 ttcttgtcgt attctgcttc gtttgcttcg tagtcacgag caaatttacg agttgcgtta
    23461 gcgaatgcga tttggcattc tgtgttaacg ttaactttgg caacaccaag tttgatagct
    23521 gcttggattt gctcatcagg aatacctgat ccaccgtgca atacgattgg gaatcctgga
    23581 agagcttctg tcaatttttg caagtggtca aggtcaagac cttcccagtt tactgggtaa
    23641 gggccgtgga tgttaccgat accagctgcc aagaagtcga taccagtttc aaccattgct
    23701 ttagcgtctt cgattggagc caattcacct ttaccgatga ttccgtcttc ttcaccaccg
    23761 atagtaccaa cttcagcttc tactgagata cctttagcgt gtgctttttc aacaacttct
    23821 ttagccaatt taaggttttc ttcaactgga aggtgtgaac cgtcaaacat gattgaagta
    23881 taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag gtggatagct
    23941 actggtacag tgatacccat tgattcaaca aggttagcga tcaagttgcg agcaactttg
    24001 taaccaccca tgtatttagc agcacccatt gaagtttgga tcaaaactgg agctttttta
    24061 gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt aaatccacca
    24121 actgcataac cgttgtcacg ggctgcttgg acaaattttt ctgctgaaac gattgccatt
    24181 ttatcaggcc tcctgtatat ttttatgggt catcccattt acattgttca ttttatcact
    24241 ttttgccaaa aaaatctagt ttttcccgca gtttcgattg attttcttct aactccatct
    24301 atgtaaaccc tttctctccc tagtcttgga cgacttttgg aaaatctata aagaaggtta
    24361 aacgattctc ctccatctcg aaacgataag ctaatttttc atgttctaat agactcttaa
    24421 ccacaaagag ccccatacca gaccccttga ccttgcgact ggcattgtca gaaaaagact
    24481 gggctagttt ttcttgttcc tctgagctac agctattttc gataaaaagt tctccttctc
    24541 tttctccaat tcgaactaag ccacctggaa cagagtgctt aatggcattg ctgatgagat
    24601 tagaaagaat caacttcata actgatgggt ttagataagc ctgctgatgg gtcaaactat
    24661 tgtctatctg gagctctctt tccttggcta gcaaggcata atctttgacc agattttgcg
    24721 tcatctggag gaggtcaatt gtttccctat catctcgcaa ttcctgcaca gaagagaggg
    24781 aaagtatctg cagaacatgg tgattgagtt catccacaat ccccaaggca actcccagat
    24841 actggtctct attcttataa cgaccgatat tctctctcat attttcgatt aggattttca
    24901 aactagccag cggtgttttc aattcatgag aagctcctcg taggaattcg accttcatct
    24961 tctccagctg gagaatggct tcattctttt catgcaagtc cgcaataaca gtcaagagat
    25021 gctggtagag gctattgatt tgttccttga gattgcctat ctcatcctta gaatccacgc
    25081 gcaatcgcac ttgggaatcc aggtccatca tccgacgggt cacccgcttg atttccaaaa
    25141 tcggtgcaac aatagtccga gcgtagatgt aggccaccaa gagggaaatc agaaaggagg
    25201 ccagcaaggt atagggaaga aactggagac tgatttgctc cgcttccttt tgtaaatcca
    25261 tggaagctag aaactggaga atcatagtac caccgtcttg cgttttcacc tcgcgctcct
    25321 caataaagag agaggttgtc tggcggtctg tgtccagagg aagactgtcc ttgacttcta
    25381 acttgtcctc ggtcatctca cctttgacgg tccccttgat atcactagtc tgggaataca
    25441 agtctaacac ttgctcgata ctctgcctat ctttcccttc tagggactgg gcaatggctg
    25501 ttgccttttg accaatggtt tcctgacgat gactcagata agtcgaagga aaaagaaaat
    25561 aaatagctaa atgaaggcag ataaccagaa cactaaatat cgagaaggta tagataaata
    25621 tctttgtaaa taaacctgtt cgtttcattt tcgctccaat ttataaccaa cattgcgcac
    25681 agtgaggata caatccaagt ctagcttttt ccgcaattcc ttgatataaa catcaataac
    25741 acggtcaaag ggaacctcat ctgtcgcttt ccagacggca tcgataatct gagaccgagt
    25801 caaggcccgg ccttcatttt tcactagata gtccagaatt tccaactctt tggcattgat
    25861 aggcacttct tgacctgcga ggcttgcact gtagctttca aagtccacct tggtatcctt
    25921 gtaagaaaag attcgtcctg tatcgtagta gcgcttgaaa atcgcgtcca ccctcacttt
    25981 taagagggag agggagaaag gtttttccag atagccatct gccaaagagg caaaggcact
    26041 catcttgtat tcctcatcct gaaaagctgt caacatcaag acaggaacct gactggtttt
    26101 acgaatctca gctaggactt ctaagccgtt aagcttgggc atctggatat ccagtaaaac
    26161 cagggccacc tcatagctag aaaattgctc cagagcttcc tgaccgtccg ctgcctcaat
    26221 agtttcatag ccacaatccg tcaaataatc actgaccccc tcacggatca tctcttcatc
    26281 ttctacaatt aaaattttca tactttaact gctctctatt ttttattttt cttagaataa
    26341 atacctactc tattttctat tatagtctct tgctggcctt ttgtctgtaa gcaactgacc
    26401 actagataaa acgttgtgaa attcctttct cataaattcc ataactttag tatattatat
    26461 ttaagcacta aagtacaaag aaagcaactg aaagcaatga ttttcaccac tgctttcaga
    26521 tttattttga attgttaaat agctattcct atccactatt cttgaataga aacacaagat
    26581 gcaatcttta ttccagactc atttttttca aatttattca ccatccagca agagctcttt
    26641 tggttgtttt ctaaggagat tgcttgaagc aagcgccata acgagaacca ctagaaccaa
    26701 ggcaaggaca aaaatgatga taaagtctga tgtctgaatg gaaatgtcta ggctcgacaa
    26761 ggtcttgcta aagccatcta cttctgcacc gccaccaagg ttagaggctt gagccgcctt
    26821 actagcctgt ttggcaacac ctgaagtcac attggcaagg acagtgtttc caattgcacg
    26881 ggcagtgtaa ttagctagga agtaagcaga aactagagca gggatagcaa tcaagataga
    26941 ttcggtgatg aattgaccca agatacttgc ctgcttgaga ccaatagaga ggaggattcc
    27001 cacttccttg cgacgggcat tgatccaaag actgagcaag agggcaagga gaaggactga
    27061 gaagctcaag ctaccccaga agaggaggtt ggccatcttg tacataccag agatagattg
    27121 ctcaagagct gggtagttag aggagctctt gacgagtgtg tagctcttcc agttgatacc
    27181 actgatgcca ttcaactctt tcataacatc atccaagttc ttgtctgctg ttacaaagaa
    27241 ggttgcgtcc ccataaatgg ctgtgtcttc tgtgtatcca taaagttttg cagcagtgtg
    27301 aatgtctgta atagctgtgt tttcgtaaag ttcttgtgag taggttactg ctgacttatt
    27361 atgaccatca aagagtccct tgattgtcac ttcaactgtt tccttggctc ctttttcatt
    27421 atctgcatcg tagatattag agtccagttt aaccttgtcc cctactttcc agccgtgttt
    27481 ggctgccaag tccttgtgca agaggatttt atccttgtcg tcgttggtta agtgctctcc
    27541 ttcgactagt ttataagaac cagagacaaa cttatcttct ttagaggagt cattgacacc
    27601 tgtaatcatc aagctacttc caaaacgttt ggcacgatca gcagtgagat tcttcttggt
    27661 ttctggcgtt tcaatcaggt catatccagt caaatctccg atagcgttga tacgtttgac
    27721 ataagactca atggccttgt tttcggtgat ttttttgatg tcttcaccct tgatattccc
    27781 agcaccacga ggcgttcctt ggttgacgcg acgattgatt tgcatggaga agctattggt
    27841 gatattttta aaggtctcct gagaagcctt ggcagtagct cccttgattg acaagccgac
    27901 caaactcaag ctcgccataa ggagaataat caggaagatg acaatcgatt tgaaaaactt
    27961 ccttgtaaca taggcaaatg cgttgtgtaa catagattcc ctttctagat tttgttttaa
    28021 tcattctatc aaaataagct caaattattt actagtattg cgcgtttcag tcagtttctt
    28081 atcctttaat tcaagtgtaa tatctgacgc ttgtgccact tctttactgt gagttacgac
    28141 aatcacacat ttacctgttt tctgggcaag tgatttgagt agttcgacaa tatctccagc
    28201 agttttagga tccagatttc ctgttggctc atcagctaga ataactggag cttctgagac
    28261 caaactgcga gcaatggcaa cacgttgctg ttgaccacct gataactgga gaacattccg
    28321 cttaatctgg ctttcatcca aaccaagctc aagaagtgta ttcttgcttg cctttttgtt
    28381 gaccaattgg atattttcca gcggagaaag ataatctatc aagttataat tttgaaagac
    28441 cagggaaata tggtgcatgc gatggtaaga atagcccttc ttacgaatat cctctccttg
    28501 aaaaaggata gaaccttcaa caggactatc tagaccagca agtagggaca agagtgtgga
    28561 ttttcctgct cctgactccc caataatact gtaaaatttt ccgggttcaa aattataatt
    28621 gatctgatat aggactgctt cagcagtatt cttataacgg taggtaacat cttgtaattg
    28681 taataaagtc atgatttctc cttcttaact aatagatgat aaaatttctt tcggtgattt
    28741 tctaaataag aataggaaac aaagggctac agataagcaa ctaagcagaa ctagaaaaac
    28801 ataggattct gcaaaagata agatgctagt tgataaactg cttgctttgg ctagtgtatc
    28861 ttgtaagctt acctgatctc cacttgctag tagagtttgg agtaggtaag ttgtgattcc
    28921 gtttcctgca acaaatgctg gaagcaaagc tccaagagat accaaaacta cctctaaaca
    28981 gaattgtagg aagatcgagc tcttgccttt tccaagtgca agtaaaatcc ccacttcata
    29041 gacccgttct ctcaaccaga gagacaaaac cagaattaag gctccagctc ctgctatcaa
    29101 catcccataa aggaagatgg tcaggaaggt ttggaaagtt gcaactgagt ctttgatttg
    29161 ttcaaaagcc ttgttttcct tttcgacttg gtagccttga ttttccaagg ccaagttttc
    29221 tacctgcttc atgagtccgt ccatttcctt aggattttct acatagaagc gtgctgcact
    29281 gacttgagct tcactattgc ccaaaagggt ttggctactt tcataatctg taaagacttg
    29341 attttcactg aagtcagaag acaagcctgt gaatttctct tgttttttac cagaaaagat
    29401 gccgataatc tcaaactcta ctgtttgtcc ttttccagat tcagactgac cagcatccaa
    29461 gccaatcttg tcatgaagtg aaagaccgtt cttcttagcc aattcttcgt ggataaggat
    29521 tttcttggaa tccccttttt gaaggtgtcg cccttctttt agattgaaag ccgaactggt
    29581 aaaggttaca tccttggatg aatcctcaag agccgttaag ctaaccaagt tattgtctgc
    29641 agctgataaa tcatcacgct ccacgctctg ctcgccagtc actgcttcct tgtcttttag
    29701 ttttgcgacc gtctcaagtt caggagagac attttccagc cccttaatct tgcttacaga
    29761 tgctaggtct gacaacttga atgtctgacc attctctatc ttcttaatag aaaaagatgt
    29821 attgagtgat ttataaagat tgctttctac tgttttgttg gacttcatca gagtcaaaca
    29881 ggctgaaatt ccggccaata agaccaataa aatcagaaat aaaataaaac ttctcagtcg
    29941 ctttctgctg acataagccc aagatctttg gattggattc atttgtcacc tccatatttg
    30001 taagactatt ataaaaccca aatatgaaat gtttatgaaa tacgaaaaaa aa
//
LOCUS       DAVBDG010000022        29055 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBDG010000022 DAVBDG010000000
VERSION     DAVBDG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29055
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.022"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(59..1762)
                     /gene="ilvD"
                     /locus_tag="VQ526_000961"
     CDS             complement(59..1762)
                     /gene="ilvD"
                     /locus_tag="VQ526_000961"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2554699.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(1995..2927)
                     /locus_tag="VQ526_000962"
     CDS             complement(1995..2927)
                     /locus_tag="VQ526_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2554700.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDVVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSAVCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2924..3781)
                     /locus_tag="VQ526_000963"
     CDS             complement(2924..3781)
                     /locus_tag="VQ526_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2554701.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNSGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3785..5131)
                     /locus_tag="VQ526_000964"
     CDS             complement(3785..5131)
                     /locus_tag="VQ526_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2554702.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGASFIKKPSAKEE"
     gene            complement(5144..5428)
                     /locus_tag="VQ526_000965"
     CDS             complement(5144..5428)
                     /locus_tag="VQ526_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2554703.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5432..7462)
                     /locus_tag="VQ526_000966"
     CDS             complement(5432..7462)
                     /locus_tag="VQ526_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2554704.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7653..8660
                     /locus_tag="VQ526_000967"
     CDS             7653..8660
                     /locus_tag="VQ526_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2554705.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8663..8863
                     /locus_tag="VQ526_000968"
     CDS             8663..8863
                     /locus_tag="VQ526_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2554706.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            9015..9197
                     /gene="rpmF"
                     /locus_tag="VQ526_000969"
     CDS             9015..9197
                     /gene="rpmF"
                     /locus_tag="VQ526_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2554707.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            9213..9362
                     /gene="rpmG"
                     /locus_tag="VQ526_000970"
     CDS             9213..9362
                     /gene="rpmG"
                     /locus_tag="VQ526_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2554708.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9784..11664)
                     /locus_tag="VQ526_000971"
     CDS             complement(9784..11664)
                     /locus_tag="VQ526_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2554709.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASMIY
                     TT"
     gene            complement(11658..12527)
                     /locus_tag="VQ526_000972"
     CDS             complement(11658..12527)
                     /locus_tag="VQ526_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2554710.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILYKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(12611..15256)
                     /locus_tag="VQ526_000973"
     CDS             complement(12611..15256)
                     /locus_tag="VQ526_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2554711.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGHPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(15347..16627)
                     /locus_tag="VQ526_000974"
     CDS             complement(15347..16627)
                     /locus_tag="VQ526_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2554712.1"
                     /translation="MIYSKEIVREWIDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            16798..18882
                     /locus_tag="VQ526_000975"
     CDS             16798..18882
                     /locus_tag="VQ526_000975"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2554713.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QGGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGKAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLNKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(18922..20601)
                     /locus_tag="VQ526_000976"
     CDS             complement(18922..20601)
                     /locus_tag="VQ526_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2554714.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGSLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIYVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            21357..22586
                     /gene="arcA"
                     /locus_tag="VQ526_000977"
     CDS             21357..22586
                     /gene="arcA"
                     /locus_tag="VQ526_000977"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2554715.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            22644..23660
                     /gene="argF"
                     /locus_tag="VQ526_000978"
     CDS             22644..23660
                     /gene="argF"
                     /locus_tag="VQ526_000978"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2554716.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            23825..24772
                     /gene="arcC"
                     /locus_tag="VQ526_000979"
     CDS             23825..24772
                     /gene="arcC"
                     /locus_tag="VQ526_000979"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2554717.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            24983..26494
                     /locus_tag="VQ526_000980"
     CDS             24983..26494
                     /locus_tag="VQ526_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2554718.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            26516..27847
                     /locus_tag="VQ526_000981"
     CDS             26516..27847
                     /locus_tag="VQ526_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2554719.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGIKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVKLLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            27942..28069
                     /locus_tag="VQ526_000982"
                     /pseudo
     CDS             27942..28069
                     /locus_tag="VQ526_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(28252..28542)
                     /locus_tag="VQ526_000983"
     CDS             complement(28252..28542)
                     /locus_tag="VQ526_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554720.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(28530..28781)
                     /locus_tag="VQ526_000984"
     CDS             complement(28530..28781)
                     /locus_tag="VQ526_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2554721.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
BASE COUNT         8565 a         6423 c         5526 g         8541 t
ORIGIN      
        1 aaccatacct acggcaaggc gacgttgacg tgatttgaag agattttcga gtatgagttt
       61 attttttacc tgacttgtcc atattccaga agtctgtcac ggctccgcgt gaagcagatg
      121 atacgatgtg ggcatattta ccgaggacac cacggctgta aagtggtggc aaggttgttt
      181 ctgccttgcg tttttcaagt tcttcttcgg atacggccat ggaaatttct ttggtatctt
      241 ggtcaaccgt aacgatatcg ccggtacgga gataggcaat tggtccacca tcctgagctt
      301 caggagcgat atgtccaaca accagaccat aagtaccacc agagaaacgt ccgtccgtca
      361 agagggccac cttatctccc tgacctttac caacaatcat tgaagaaagt gacagcatct
      421 caggcatacc aggaccacct ttaggtccaa caaaacgaac aacgactaca tcgccatcaa
      481 cgatttcatc tgtcagaacg gcctgaatcg catcttcttc tgagtcaaag accttagctg
      541 gcccaacgtg acgacgcact ttaacacctg ataccttggc aactgcaccg tcaggagcaa
      601 ggttcccgtt caagatgata agcggaccat ccgcacgttt tggattttca agtggcatga
      661 taactttttg gcctggagtc aagtctgcaa agtcagccaa gttttcagct acagtcttac
      721 cagtacatgt gatgcgatct ccgtgaagga aaccatttgc caacaagtac ttcataaccg
      781 cagggacacc accgacttcg tagaggtctt ggaagacata ctgaccagat ggtttcaagt
      841 cggccaagtg aggcacacgt tcttgaatcg tattgaagtc ctcaagtgac aagtcaacat
      901 ttgcggcatg ggcaatggcg agcaagtgaa gagtggcgtt tgtagaacca ccgagagcca
      961 tcgttacagt gatagcatct tcaaaggctt cacgagtcaa gatatctgat ggtttgagac
     1021 caagttccaa catcttaaca acagcacgtc ctgctgcttc gatatcttct ttcttatcag
     1081 ctgattcagc tgggtgagag gatgaccctg gcaaactcat ccctagaact tcgatagcag
     1141 ttgccatggt attagcagta tacataccac cacaaccacc agggccaggg caggcattac
     1201 attcaagacg tttcacgtcc tcagctgtca tgtcaccgtg gttccatttt ccgatacctt
     1261 caaagacaga aaccaagtcg atatctttac catcaagatt tcccggtgca atagttccac
     1321 cataggcgaa aatggctggg atatccatgt tggcaatagc aatcatagat ccaggcatgt
     1381 tcttgtcaca gccaccgata gcgacaaagg catcgacgtt gtgaccactc atagccgcct
     1441 cgatggagtc cgcgataatg tcacgagatg ttagagagaa acgcatacca ggcgttccca
     1501 tagcgatccc gtctgctacg gtaatggttc caaactgtac aggccaagcg cctgcagatt
     1561 tgacaccttc tttagccagt ttcccgaaat catgcaagtg aatgttacat ggtgtatttt
     1621 ctgcccaagt cgaaatcact cccacaatcg gtgtttcaaa gtccttatct gtcataccag
     1681 tcgcacgaag catagcacgg ttaggtgatt taaccatgct gtcataaatg ctactgcggt
     1741 gacgtttatc taattcagtc atcttatccc tcccatttca gtttttacta ttatagcaca
     1801 atttaaaggc aatgaacaga ataaaattct tgaattttca gaaaattcta tacgcatgtg
     1861 aaatatttaa aattaaaaac aacaaagcgg attagtgcac tttctgatga ccagaatatg
     1921 ctttttaatc cgctttcttt aaataacgta ctgtaatttt tacagaaatt ctttcaaata
     1981 agtgtattta acatctatct tgcattataa atttctagaa ccttctcttt tatattcgat
     2041 tcactcaaac catactcatt aagaagataa tccattttcc ctacttgacc aaatctttct
     2101 tgaacaccca tccgatgaat ttttgttatt ccatcatcag agaataattc acatacagca
     2161 ctgccaattc cacctatctg attgtggttt tctacagtaa atatagtttt tccacttaac
     2221 attgttttta tctgttctgg tatcggtttg attctaaata aatctatcac acctactgaa
     2281 taacctaatt tagacagttc atctgcaact cgaatacttg gagcaaccat tatgccagaa
     2341 gcaacgatta caacatcttc accatgcctt aactcaatgt agcctttaga aaaatcttct
     2401 ccaccttgat acacaggaac tggagctttt ctaattgttc gaatatattt tagtcctttt
     2461 aagtctaatg tctggttcaa tatttcacga aattggatat catcagttgc ttcaaaaatg
     2521 atcgatttag gaattaaacg taacaatcca atttcttcaa atggcatatg tgtcccacca
     2581 ttcatttctg cagttactcc tgcatctgat ccaatcacag tggcatccaa ttgtgcgtat
     2641 ccaagagaaa taaataattg atcaaatact cttcgtgaag caaaaggacc aaatgtatga
     2701 agataaggtc taaacccctg aatagacaag cctgctgcaa ggccgaccat ttctgcttcc
     2761 ataatcccaa cattcacata acggtctcca aagtcctttt caagattatg agtagccatc
     2821 gaacttgaca aatcggcttc taagactact atatcagaat cactttgatt agcctctaaa
     2881 aggaagtctc tatatacatg ccgtaattct ttcgtacttc tcatcattct gtttcctcca
     2941 attcctgact taatctttct acaactgaag ttaacatttg tttctcctct acagtagggc
     3001 gaagatgatg attggatttc atttcttcca gctcttgaac cccttgacct ttaatagtat
     3061 ctaatacaat gcacttaggt gatgaattat ttgactgttt taattggaca atcccttcat
     3121 aaatttctct aatatctgaa cctttgaccc taatggattc aaatccaaat gctgaaaatt
     3181 tttctacgaa atcacctgaa ttacaaatat cctttgtaaa accatctaat tgttttttgt
     3241 tatcatcaac aaatacaatt aagttggata actgttgatg agaagcaaac tgtatagcct
     3301 cccaacattg tccctcattt aactcaccat ctccaacaat agcgtaagta taaaagggac
     3361 tctttcttat tctctgacca tatgcaagtc cagttgcaac actaattcct tgtcctaaag
     3421 agcccgttgt catatctatg cctggcgtta gatttctatc aggatgagac ggtaatttgg
     3481 ttccatttgt atttaaagaa tataagaatt ctttgtcaaa gaaaccattc aaatagagtg
     3541 tactgtatag agctggtcct ccatgacctt ttgataatat gaaataatct ctatctcgtg
     3601 ctgcaaatat ttctggagtc attggcatta tttcaccata aagcactgct aacacttcta
     3661 cgatagacaa acttcctccg taatgtccga atccaagatg attcaatgtt ctaagagtat
     3721 ttaatcggat gttagtcgca aattttctta acccatcttc tctattttta cttaaaatca
     3781 tcccttattc ctcctttgca gatggctttt taataaagga tgctccaaac ataactgcta
     3841 aaataagaac aagaccaatc acaatgcctg cttgtgagcc aaattgattt aacattccta
     3901 aaataattcc tgatagacca aaatctgcat ctgagaaagt tgatccttgg aaaccaagtc
     3961 ctcccaaaac tggcattaaa aagactggaa gaaaactgat taaaatacct tgtaaaaatg
     4021 ctccaatagt ggctccacga acaccaccag atgcattccc aatgacacct gcagtcgctc
     4081 cacagaagaa atgaggcaca acgcctggta agataacaac cgttcctgaa gcaatcataa
     4141 ttgccatact tactaaacca ccaacaaaac tagagataaa tccaattaga actgcattgg
     4201 gtgcataagt ataaacaatc ggacaatcca aagcaggttt tgaattaggt acaagacgct
     4261 ctgaaatacc tttaaaggct ggaacaattt cgcccaaaat aaggcgaaca cctgctaaaa
     4321 taacaaatac ccctgctgca aattgacctg ctaattgtaa agcataaact agaccacttg
     4381 taccactact gatttctttt tctatatatt ctgaccctgc aaagatagct acaataatgt
     4441 aaataactgc catggataaa gtgatactaa cagtactatc acgtaaaaaa gctaaactct
     4501 ttggaaattt aatgtcctct gttgattttg atttgtcacc gataaggcta ccagtaaaac
     4561 cactcaacca atatcccaaa gaactgaaat gacctaaagc taccttgtca tttccagtta
     4621 attgaaccat atatttttgc acaaatgctg gggaaatact cataataata ccgagtgcta
     4681 atcctcctag taagataaga ggcaagctag taaagccagc aactgataaa atgaccgcaa
     4741 tcatacatgc catatataga gtgtggtgcc ctgttaaaaa aatatattta aatcgagtaa
     4801 aacgagcgat taagatattg aacaccatgc ctgcaaacat aatcattgca gtagctgagc
     4861 catatgttgt taaagctaca gctacaattg cttcattatt cggcacaacg ccagataaat
     4921 gaaaagcatg ctcaaacatg gtaccaaatg gatttaaaga attttgtaca attcctgcac
     4981 caccagatac aactaagaaa ccaacaaagg tcttaattcc acctttaata atatcaggta
     5041 atttcttctt ctgaagaact aatcctaaaa ttgcaattaa agctactaaa atagctggtg
     5101 tactaacaat atccaatatg aacttcatca tgacgctagc ctcctatata agtccttttt
     5161 cttcacaaag tttagtaatt aattctcgta gttcatccat atcaataata ctatttaaga
     5221 tacgaacatc tccaagatga ctagctgaat cagctagatc acgaccaaca atccaaatat
     5281 cagctgcatt tggatctgct ccacctaaat cataatgttc aacttctaca tccgaaacat
     5341 tcaaatcact caatacagat tcaatattca tctgtaccat aaaacttgaa cctaatcctg
     5401 aaccacaagc tgtaccaatt tttaacatta tctaatcctc ctgtttaatt atcattttaa
     5461 tgtcatcata gttttttgat gatattaaag tttgaacatg atttttatct cttaaaattg
     5521 ttgttaaatg tgacaaagcc tttaaatgac tctcattatc aatggctgca atacaaatca
     5581 acaatcttac ctcttgttct ggattatcca ataaataaat cggttcttcc aaaactaaca
     5641 ttgacattcc tatttcattc acaccttcat ctggccgagc gtgaggaatt gctactccct
     5701 tccctaaatt aataaaaggt ccaaactctt ctactttttg aatcattgcc tcagggtagt
     5761 tctcagttat cttatcttga tccaaaagcg gtttagctgc taaacgaatc gcctccttcc
     5821 atcctaattt ttgcgaacta acctgatagg tttctttggt aataagttgt tctagcactg
     5881 gtacaatttc ctttctatca tttttttgat aaagataatt ctttaacgcc aatcttaatt
     5941 ccaattcttg tgtaataatt ccatatcttt tgacaatatt caggatttgt tcaatctcaa
     6001 aatctccata ctctaaattc ggaaaatctt ttaacactag ttctactagt tgtattgctt
     6061 gctcttcagt catcataacc gaaactagat aatttggctt ttctgtctcc acctttatgg
     6121 tagaaaaaac catatcatag tcactactag ctttcacctg taaatcatca atctttgagg
     6181 ttcctataaa ctcaatttga ggaaataatg ctaatagatt ctcttttaac atcaatgaag
     6241 aactaacacc attaggacaa atgattgctg ctttatagca tttttgaggc aaagtatctg
     6301 ctttctttaa ataacctccg aaatggataa caaaatatgc tgtttcacta tcaggtatgg
     6361 gattgtcaat agcgtccatc aagggcatca aagaatcttt gactaattca aataaatcag
     6421 gataatgttc tttaacatgc aatacatatt catttgaact aggtaggccg aactttaatc
     6481 tatagtaagc cggtataagg tggcggcgaa gattttctct caatccttcc ctttgtttaa
     6541 aatgtaacaa agaaatatct tccattctac ttataatagc ctctgttaat tgattaaagt
     6601 aaaccggagc aacatctact tcaccttcaa agcaacttga taataaaact gtgatatagc
     6661 gataatcatc ctcagaaaac accgtatcta taattcccaa atcaaccact gtatccaata
     6721 aaatagtggt tatatcttga ataacaggag atactaatgt ctctgaaaga catactcttt
     6781 caacatccct ttgataccta cacagaatga atactaaacc gaaaaggcaa acttttaatt
     6841 gattaacaat aggtactagc tgtagcttct cataataatc tttaactacc tgatttatca
     6901 aatcataagt taatgaatac ccccaactgg ataaaacata atccaaaccc caaatcccta
     6961 tggaggattc cagcaactca ctaaccattt gaaaagctaa gcggtgctta ttccactctg
     7021 aaccgtgtaa agtataacct tttgctctac tgtaccctag ctccaaatca ttatctaaca
     7081 taatctttct taatgattga atatcagata aggttgtatt cttacttact ttcaaaaagt
     7141 cttggtaatg attattcgat ataaaatcta atcggcaaaa agtgtaaaga tagattaaag
     7201 ctaagcgagt cgactttggt aaaaccaatt catccgactt aataatatct gtcaaagact
     7261 gcttcgtacg atttgataaa ctatagcgac cttgcttttt atccagcact atccctttat
     7321 tagctagata aggcactaaa taatctattc cttctttgac ttcctttata ggtaagctca
     7381 ccttaacaga taattcatat aatgatagct cacaatgatc catcaaagtc atcaaaataa
     7441 ctagtgctct ataatcaaac atttctcttc ctttctaact acaattatag tgtaaaagtt
     7501 ataagaataa aagagtattt gagcacaata taatctcacc ttttaaaaga aattattggg
     7561 ctgaaagttt ttagtgatta ttttcaactt tctattgact tttaaaaaca aagtgatatc
     7621 atttagatat caaataatgg gaggtcattc ttatgactga aaaactaatc aattcaaaac
     7681 caaatggtgt attcgcattg attctcattg agttgacaat cgtacttggt atctttatat
     7741 ttataatggg tgttggttcg gaaaacattt ttggaattat tatcggacct ttactaatcg
     7801 taattgcagg tctagctcat gctggtttaa aagttgtcaa acctcaggaa gctctggttc
     7861 tgacactctt tggtaattat acaggtacca tcaaagaacc tggtttttac tttgtcaatc
     7921 ccttcagcgt agcagtcaac cctgcaaacc acactcgact tggacaaagt ggtgatgtta
     7981 gcacaaaatc tcctttttta ggagctaaat catcaaatga caatgatgta aatcttgaaa
     8041 ttggcaagaa acacatttcc ctcaaagtca tgaccttgag caattctcgt caaaaaatca
     8101 atgattgctt aggaaaccct gtagaaatcg gtatcgcggt aacttggaga gttgtcgata
     8161 ccgctaaggc agtcttcaac gttgataact acaaagaata tctttcattg caatgtgata
     8221 gcgccctccg taatattgtc cgcatctatc cttacgatgt gtctcctaat gtggatacta
     8281 cgggtgacgg gcaagcagat gaaggtagtc tccgtggctc tagcgaaatt gttgctaacc
     8341 gtattcgtga ggaaattcaa agtcgcgtag aagatgctgg cttggaaatc cttgaagcac
     8401 gtatcactta cctagcttat gctccagaaa ttgctgccgt tatgcttcaa cgccaacaag
     8461 catctgccat tattgatgca cggaagatga ttgtagatgg tgctgtagga atggttgaaa
     8521 tggcactaga acggctcaat gaaggggaat tggtagaact tgacgaagaa cgaaaagctg
     8581 ccatggtttc aaatctcctt gtcgttcttt gtggcaatca tgatgcacaa ccaattgtca
     8641 acacaggaag tctttactaa agatggctga agctaaaaaa aagcagatcc cccttcgact
     8701 ctcaacaaag ttatacgctg cactcgcatc atgggcagaa gacgactttc gttcagtcaa
     8761 tgggcaaatc gaatatctcc tcacagagtg tgtcaagcaa cggaaaaaag acggaaaata
     8821 cgtatcagaa accattgacg aaccatttga gattgatatt taacaccttc tcctgtctgc
     8881 cgtgtcggat aggagaattt tttcatcttt ttttgtcaag taactttact ttacaaaaaa
     8941 aatgtgttat cctagtatgg ttgatgaaaa tcagtagatt gaatcgaata taaataccta
     9001 aaggagaaat caaaatggca gtacctgcac gtcgcacttc aaaagcgaag aaaaacaaac
     9061 gtcgtacaca ctacaaagta acagctccat ctgtaaactt tgacgaaact actggagatt
     9121 actcacgttc tcaccgtgta tcacttaaag gatactacaa aggacgtaaa atcgctaaag
     9181 ctgcatcagc tgaataatag aagggagata ccatgcgcgt aaatattaca cttgaacaca
     9241 aagaatctgg tgaacgcttg taccttactt ctaaaaacaa acgtaacact ccagaccgcc
     9301 ttcaattgaa gaaatactca ccaaaacttc gcaaacacgt tgtgtttaca gaagtgaagt
     9361 aagcattcaa tacgaatcaa tacagaagaa aaacgcttga tcagcgtttt ttctttttgt
     9421 taatttcact atattgcaag ggaaatcatt cgaatgacta cattattgat aatatttgtt
     9481 aagccatcaa aagtaattaa catttcatag cgagtaatcc tatttaaact cgtcccacta
     9541 tctatttctt cttatctctg tgttataatc ccgactttat cataagtttg tctaactgta
     9601 gcatttgaaa ccagataatt cttctgtttc acgcgaaaca ccctgcgcca agcaaggtgt
     9661 tcttgttttt atactcaatg aaaatcaaag agcaaactag gaagctagct gcaggctgct
     9721 caaaacactg ttttgaggtt gtagatggaa gctgacgtgg tttgaagaga ttttcgaaga
     9781 gtattaagtc gtataaatca tcgaagctgt cgcaatggta tgccactggt tagctgtagt
     9841 tgctgcgaaa tccttgtctg cgtagaagtc agtaaatggt aggatttcaa cttccagctc
     9901 gtcgatgcgg tcaagctggc cagccagata aacctcgata cggctttctg ctcgtttgat
     9961 gcgttgcaag agtccgccca tacggatgtc aactgtatcc aaaccaaaga ccttgttttc
    10021 tttcagccat tggtgactaa agagggcatg gaagtcttca atttggcttc taagttctgg
    10081 taattcttgt ctggcgattt gttgtaaact ttctttatca tccgcttggt aggcctgacg
    10141 aatgcgtcgt cccacatcta ctttgctact taaaatagca ttcaactggg cctgagtttc
    10201 aaagagataa gcatagtttc cagctttttc tttaatgtta gcaagcgtct cagcagcctg
    10261 agcgaagtgt ggcttgtcct gttcaggtgt catgtgtcgg tcaagtatcg gacagagaac
    10321 atcctgataa aagacatagc ggttgggatt gatgccactg agattacctg gtaggtctgg
    10381 taagaggttg gcaagatcaa tctgcataaa atcctcaacc gttagaccag tattggtctt
    10441 gaaatgcgca gacaaactat ctaggtcatt gcgatagctg agttctgccc agatttgcaa
    10501 gcttggtagg atagagaact gggcagtttc accaccattg tctccccaac ccgttacgat
    10561 gacttcttta atctgattgg cacggcaggc tttattagcc tcgatagcca ctagacggct
    10621 aaaatggttg tgaggtgtaa agccaatcca cttccaagct ccccctgcaa atgcaaggtc
    10681 atggctaatc ttgtgatgat tgcggaaatt acggttgtat ttttcctcgc tatcctgata
    10741 ataatcccag taaaccagag tcacacggtc tttgagacgg tctaggtaga cacgagtttc
    10801 ctctggaatt tccacatcac ggtcgtactg gccatccgct gacatgagtt tgaagaacat
    10861 atcactccac atctggcagt ggaaaccata tttgtcagca atatccagca cgcgctccaa
    10921 gtgttggcac atgaggagac tacgatccac aacaccgttc aaaatcaggt agcgtcccaa
    10981 accaaccaag tgggcttcgt ccatcccgat attgaccttg cgagtcttca gtttagacaa
    11041 cgtggcaaac atgccatcaa tcaggtcata aaccttttct tcgccaatga gaagaatgtc
    11101 ctctacatca cggagctcct gcacttcctt gacaccccat ttgacaaagg ccgacaagtg
    11161 ggccaaggtc tggatgcatg gtacaaaggt catgtcaaac tgttgggcat aggcttcgat
    11221 ttcctgcaac tcctctgctg aataagctcc acggaaatag ccaaagtaag gctgcccttc
    11281 aatctggtaa gtgtcttcca tgtaaagctc aaaggttgag tagcccatga gagccaagac
    11341 ctcaatcatc tgcttggcag aagccacatt cagcaccgca tttcgagaac agtcaaccat
    11401 gtaagccaaa tcttcgtaag ctgcttgttc ctcaatctct actttatcag cttctgctag
    11461 aactgttacc aacaaggata aggcacgata aagttggtga ggtttgcggt aggtcaattg
    11521 atagtgacct tcctcaccct cgatagagat agaggcttgg tcagactgag tgacagccac
    11581 ttccacatct ggtagagaaa tgtgaccttt taaaacctca atagcttgcg tttgtttggg
    11641 actaagtcct gtaaatctta ccattgcttt tcctcctgta gccagttgac aagagcaccg
    11701 tagagattgg catctgcgtg ataggtgcag gcctggataa ctggtgcgac cgtgtattct
    11761 tcgtaggtat cgacaaagtt atcaacagcc ttcttgacat cttggataaa atctggattt
    11821 tgactgatag agccacccag actgatgaca tctggatcga tgagatactg gatattgagc
    11881 aagccttgcg ccagattgcg gttcatgcgc tcaatggctt cttgacaaag agcattacca
    11941 gctgcggcct cttggtaaat cttgcgaccg tcccaatcag tatgaccaga tttttcaatc
    12001 acgtatcgta ccatattccc agttgatgct agttgcgacc agttattaag tttttcagca
    12061 ggggcaaggg ttgtcatgta gccaaattct ccacccagac cgtggcgacc tcgatgaagt
    12121 ctaccattga taatcatggc tccgccaatc cctgtcccaa tcacgacaca ggctgcattt
    12181 tcaagctctg gatgagctag tagttcactg agtccaacgc agttggcatc attttctaaa
    12241 tggacaggta gctgataaga gctaagcgcc tcataccaag aaaagccatg gatgtagggc
    12301 accgcactga agccatcaat cacacctgtc tcttgattga ccgcacctgg aacgctcata
    12361 gcaatcccac tgtaatcctg ttctgacaag cgttgatcta gccacgctag taaatcctcc
    12421 aagttttcag gcgttgaaat acttgtctta tacagtattt tcccatcagg agtcagactg
    12481 gcaaacttaa tcccagtccc tccgatatca atcgttgcaa tggtcattgt ttttaccata
    12541 aaaaggggcg aatcagcagt tagttcttac tttttcgccc ctcctttctt ttgatgttta
    12601 ctttttgaaa ttaaatttct tcttttttga tgaattctgt acgaatctct tgtggatcca
    12661 atggtcctga atgaactgga tatggtcgct caagtaagtc aagaatggtt tgttgatgtt
    12721 ctgatatgcg cacattttct tgactcatat tatagtaacg gaagacatag ccttcttcat
    12781 tttccgccac tttaaaggct gttggacaaa cttgcggtat gctgagaaca gaatggctca
    12841 agaggctacc agtcgcaacc acgcttcctt cctgtctagc aagctgaagg ctggtaaacg
    12901 gtgtctgcaa ggctttggca cgacggaaag ctgaaaagcg ttcttgggct tggtggcatt
    12961 caagtgcaaa ctcgacttca aactcacgca agcattgtgc ttctggcgtt gggaagtagc
    13021 cccagtcacc tagctcacct gatgcacgca aaatggtcac ggcaatggtg ttatccccaa
    13081 ggatttcgta ttcattcaat cccttattgg atacagtcac ccctttttca tcgtcataca
    13141 gactgacaaa agcttgttgg tgttgaggat tttcaggatt ttcccatgaa gcagctggtt
    13201 tgtttggtcg tgtcaccacc tcatagatac tttcagaatc attgcttgga cgcgtgttat
    13261 gagtcttgac caagagacgg atacggtgat ccttggcagt gttagtaaag cgagtcttga
    13321 agcggatttg tggattgtca acgaagacag tcaactcagt ttccagagga atgtttgtca
    13381 attcttctga ccgtccagcc tcacgcttca taaactcgat gataccttgt tgctcttctt
    13441 ctagcttttc atccgcactg acaggcacgg tcaattcatg tttgagcaag attttagcat
    13501 agcgatctgt gttttccaag acctcgtggc ccttaagctc tgcaaagatt ggctctgttc
    13561 cttttggttg gaaatagata tactcgtttc cgatgtcccc acggtcttcg aagcggataa
    13621 agtcttcata ggcttcgtga gttgtcttgt catatactgt gatgttgtca tccacactca
    13681 ccgttacgaa tggtgtatca atcactccgt tttggtaaat accctcacgg tgttcttgtt
    13741 ttccttccag caattggaag gttgtccaag aaagcggcgc taggtgaact ggaatggtca
    13801 cgcgcacttg tcgagcaata cgagcttggc ggaacttgtc ttttggtaaa tcgtactcaa
    13861 aattagctcc gaggtcttcg attgtagcct ctacaggatg accatccaag tcctccacac
    13921 ggtaacttgg caagataaga gcagccatct ttttgtagcc ttctgttggg tgcaattcct
    13981 tgaaatcaca agtcgccaca tcaatcactg tgctgacagt atcgacctta tcatgcaagc
    14041 ctgtgttaat gacagtaaag agatagtcac tttgagcctt atccgtagca attttaccct
    14101 tccactcgtt gagcaaatta cttttaacaa agtttcctac ttggttgacc ttggcaaaac
    14161 gcgtttccat ctcgcggtga acttcgtcca cgctacagcc acagatacta tcatgtggcg
    14221 cattctgcaa aagtgttttc caagcatagg tcaactggtc cttgtggttg tgtccaccag
    14281 tgataatagt caagggttct accacttgct ctaggaggtt gctattttct tggaaggctt
    14341 gttttaggta aatgcgggat gaagaagtgt tggcaagtgt gtaccagcca tctgtttcct
    14401 gactggtcaa ctcgcctgta acagttgata agtgttcagg aagcgcacct tctacagctt
    14461 gaacatattc atcaaaagaa ctatgaacaa agattacatc tgggaagagt tcatttgcca
    14521 cacgaatggc ttcgctcaga tttttctgta caggctggtg gtcacagccg ttcatcatca
    14581 accattggtt ggtcgaagcg taggcacgca catctgacaa tttttgtttc cagaaggtca
    14641 aggcctcatc tttgtcaact gggatttcat tcccattact gtaccagtta gcaaagagaa
    14701 tacctaaaac acgactacca tccacaccct gccagtacat ttctgaaaac tgggatgtaa
    14761 actgctcatc ttcaaggact tggttgtcaa atccaatcgg cttcacacca cgaccaaagg
    14821 ccgccacgtg aatgcctgat ttttgaagaa tttgaggcgc ttgtcccata tttccaaagg
    14881 tatctggaaa gtagccaatc tgggttgatt taccccattt ggcagcttct tgttgaccaa
    14941 tcaaggtatt gcggacattg gcttcactgg agatcaagta gtcatcctgc aagatgtaaa
    15001 agggaccaat tttaagtttg ccctcgtcaa tgtagcgttg gaccttgtcg cgattttcag
    15061 ggcgaatttg taagtagtca tcaaggacaa tagtttgtcc atccaagtgg aaactcttga
    15121 actcagggtc attttcaaag agatcaaaga gattgtcaaa caattccacc aactgcatac
    15181 gatggctttc aaaaggcaag taccactcac ggtcccagtg actatgtgag ataatatgta
    15241 caacaacatt ttccatgaag taaaacctca ttctaaattt aaattttaac gttttaaatg
    15301 taaacttgtg attctgataa gaatcggttg agctaaagcg agccccttag cgaatatcca
    15361 agtaatccaa gaccaactca cagaacatca tgttagccca ggagaaccat tcacgagagt
    15421 agagggtcgg atcatctaca tgaaagcttt cgtgcatgac acctgtacca ccatcgcagg
    15481 caaccagctg atccagcaag aattttttct ctgccttatc tcttgttgtc aatccttgga
    15541 tagaaagggc gattggccag atatagcgat agaaggtatg agaacttccg agaccgctag
    15601 cgtattctcc ttggtagaaa tatggatttt cagagctcaa aatggtacga cgagtagctt
    15661 gatacacttc atcatcgacc gaacagtagc ccagataggg cgcagctagt agacttggta
    15721 catttggatc atccatgatg ctggcatttc ctaggccatc cacttcaaaa gcgtaaatct
    15781 tttcgccctt gctgttggtg gtgtaagcgt agtttttgat tccttcttgg atttcatcct
    15841 gaagacgctt ggcatcagca ataacactct ggctatcagc taggtttaat gctgcgaaga
    15901 tttcttgcac ataacccaag actactacag caaacatatt tgacggaatc aagtaactat
    15961 actgacagca gtcatcgctc ggacgaaagg ctgaccaagt catacctgtc actgcaaagt
    16021 caggtccaaa gccatcattt accaaggtgt cttccttacg gtccgtgtct cggacaaaac
    16081 gataaggaga gttcttgtgg tcttgttcca ccgtccacag atggagaatt tccttagtcg
    16141 ctgcgacaaa aatctcatca aactgactag tctcgccagt ctctttccag aggagataag
    16201 ccaactgcaa aggatagcaa agcgaatcca cctcatactt gcgctcccag atccagccgt
    16261 taaggtctgt gtggtcagtc tcgtggtgcc ctttccagtt ctcctcaatg ttgaaggagt
    16321 tagcataggg atccttgagt accaaggtca tctgacgttt gaccaaacct gcaatggtct
    16381 gacgcaggag ggcatctctt ttagctacat gaaggtaggg tctgagttgg gctgtcgaat
    16441 ctcgaagcca catggcagga atatccccag tcaagacaaa agttgaacca tcttctaaga
    16501 tttcaaccgt attgtccaag gtatcggtgt agcaacgctc gaaaacatcc acccactctg
    16561 gatggtcctt agcccgctct gctacttcat ctatccattc tctaacaatt tctttcgaat
    16621 aaatcatcgt gcaattccct ctttcaaaca agtttcatgt tcagtatata gcttttgaga
    16681 cagaaaatac atacacaaat gatagtcatt tttctacaaa atagttattt ttgaaactcc
    16741 ttttctaaag gccctctttt tctgatataa tgtagaaaaa cagctaaagg aaagactatg
    16801 aaaccactac ttgaaaccat cgatacccgc tttggtactg ctagcaagca tgccttctct
    16861 cggggaaata ccctgccata cacaggcgtg ccttttggga tgaattactt tgtgccccag
    16921 accagtgacc agggtggatc ttggtttttt gatccgcatc tgcctatctt tcaggggatt
    16981 cgattaactc accagcccag tccttggatt ggggactact cttggctcct tctgacacct
    17041 gtcaccagtc aactgggggg agacagcctc tttcaccgcc agtcttctta tgatatagat
    17101 aaagcttgct tccagcctca ctatttgaag ctcttttctc tgcgttatca gattgaaacc
    17161 cagctcacac cgacttgcta tggtgcttct attcgtttga atcaaaagca aggcaaagcc
    17221 ctctccctct atcttcacgc agcagatgaa ctgacagtag agcaagtaga taagcggact
    17281 cttgccctgc gacaagaagg taaaactgaa actaacaaaa attcgctaac gatgttcact
    17341 gccctgcaaa tgaacacgga tattcttgct atcagccaag aagctggaga ctggcgaatt
    17401 gacttagcaa gtagtcaaac cgagatgcaa ctagccactt ctttcatctc tccttctcaa
    17461 gctctaatca atctacctca agaagatttt gatagctgta aaacaagtgc ccaagcggat
    17521 tgggaaaatc tcctccatcg ttttgacatt atagagacag gagaggctga ccgaaccttc
    17581 tttgaccact gtctctacag actcttcctc ttcccacaga ctttttatga ggttgatgag
    17641 tcagggaaag ccatccacat ggatctggct actggtactg tcaagcctgg tgtcctcttt
    17701 agcaacaatg gtttctggga taccttccgc accaccttcc ccctctttgc ccttatcata
    17761 ccggagcact atcaacgctt tttagaaggt ttcctcaata gctaccgcga tactggtttc
    17821 cttccaaaat ggctggctcc agatgaacgg ggtatgatgc caggtacact gctagatggc
    17881 attatcgcag atagcgcctg taaggacatg gcccccgacc tagaaggaga actcttccaa
    17941 gccatgctcg aaacagccag caaagccgac cctctcggca tcaatggccg ccacggacta
    18001 gcccaatacc aagaactagg ttacctctct accgaccacc atgaaagtgt tagccatact
    18061 ctagactatg cctatagtga cttttgtatc gccagctgtg ccaaaaaact agagaacata
    18121 gaaatcgctg aaacctacaa ggctgcttca caaaattacc gccagctatt tgacgctgag
    18181 acaggttaca tgcgagcacg agacaatcaa ggaaactttc accctgactt ctctccttat
    18241 agttgggggc gagactacgc tgaatgctct gccattcaag ctactttagg cgttctccac
    18301 gacatccctg gcttaatcca actgatgggt ggaaaagaaa cctttagcaa ctatcttttg
    18361 aaagcctgtc aagatgctcc cctctttgaa acaacaggct atggttatga aattcacgaa
    18421 atgagcgaga tggctactgc tccttttgga caaatagcca tttccaacca gcctagtttc
    18481 cacatccctt atctcttccg ctacagcgat taccctgact acactgccct tcttatcaag
    18541 acgctccgtc agaaagcttt tcacccaagt tgggaagcct atcctggaga tgaagacaat
    18601 ggtagtctct ctgcttggta catctggtca gctctcggat tttatccaac ctgtccagga
    18661 aaaccaagct atgacctcgg aatccctctc tttgaccatc tacgagtcta cctagctaaa
    18721 gaagataaat ggctggatat ccatactaaa caaaaccaca accattttaa ctttgtcaaa
    18781 gaatgccgac tgaacaaaac actcgtatca actattcaac accaagacct cttaaaagct
    18841 gaacaactaa ctttcaccct cagctggcta ccaagtcact aataaccaaa agaacctttg
    18901 catcgcgcaa aggttcttct tttatgaaac ttggacttga agttgatcca caagctgacc
    18961 atccaccgtt aggttcagat agaaatccaa ggttttatct cctgcgaaat acagggttgg
    19021 tagatgcaaa tcttttttgc tttcccctgc ttggaattga aggacataaa tctcgtcctg
    19081 ataggcgaca ccatggacac ctgtccccgg ttgaatcgaa atcttagctt ctaaaggacc
    19141 actacattct ctgcgcttaa ctgtaaaata ggcattttct ccctttgcca cagctaggct
    19201 tttttctgca aatgctagtt cctgaacaac ttcttttttt gataatccag gagttttata
    19261 aagggaaatc ttggtcaata caggaataga ttgtgcctcg gtaatcatca cgcgcacctt
    19321 ttgtgcctct actagcgaac ctcgcaagag gcgcttatga ccaactgtga agcccctgcc
    19381 aaattcctgc cagacaccat ccacctctac ttgcacatgg aaagcagaaa tacgttggcc
    19441 taacttcaaa tcttctctca actcaattac atcaaaagtt ttaggagaac ctaagtcgag
    19501 ttctaactgg atgggcaagt ctgcatcgtt tgcccaagag ctggtctcaa ggccgtctgt
    19561 caaatggcga caagcaaagt ctgcggaaag agctggacca gatacctcag ctcccagagc
    19621 caaatcttct ttatagagct cattgcgata ggtcgcaaat tcataaagtc gttcaatatc
    19681 ctttgcatca aagagcccag cttgattcgg cggaatatta agcaagagtg gagttcctcg
    19741 ccctactgag tgaaagtaga tttcgaccaa ctcctcgaga gacttaggat cctgatcctc
    19801 atggtagaac cagcctggac ggatggaaac atctgcctct ccgattgaaa aaatcgtgcc
    19861 cgagggatcc ccgtgctgaa gatagttcag ctctgcttct gttcctagtt tatcaggatt
    19921 caccttttgc cacagtggat cacctgcata ccctcgttca ttgccaatcc agcggatact
    19981 ggtgccttct gttgaaaaaa tcaagcaatc gccctgcagg tcacgaatgg tttcaaacca
    20041 tttttcaaat tcataattaa ccttttgcgc gccctctcct ctggcaccat ccatccaaac
    20101 ctcagcgaac ttaccagcat tcccatagtt aggatttgat aagatttcct tcaactgagc
    20161 cagataatag gcattgtagt ccgcttctcg gtccacatga tagaggggac tatgggcatc
    20221 ccacggtgac aggtagaccc ccatatccat atcaaactct gtggcagctt gggatacttc
    20281 aaggagcaag tcgccctttc ctctcctcca aggactgacc ttaaccgaat aatctgtgtg
    20341 agctgtcgga taaaggacaa agccatcgtg gtgcttgacc accaaaatca actttttgaa
    20401 gcccgtttcc ttgagcacac gaacccactc acgcgcatcc aactgactcg ggttaaagcg
    20461 ctcaggatcc tcctgtccag tcccccattc ttggtcataa aaggtattag gaccgaagtg
    20521 gataaaagct gctagttcat ctcccagata agctagctga gtctgacttg gtaacggtcc
    20581 atgcggtttg attttcttca tcgttatata tctccttctt tagcaacttc tcttgttcac
    20641 aaggttatcc acaggttgtg gataataaac catgatctaa cagccagctc tttccaactc
    20701 caactggtag gctcaggatt ttgctcctca atattccctc ttcaaacagc tctgtcatat
    20761 ggttctgcta acacctctca gaatgctact ctccataatt attctcaggc ttctttcagg
    20821 tcattgtgtg aagaccattc actctccaga cttttccaca gattgtgaat aagtgattat
    20881 tctaaactat cacctcgtcg actataacaa aaaataaaaa gagggtcaat aagataatta
    20941 tctcttccta aatactcaga aaagattcta taatttgtac aattgaacaa gaaatgcttc
    21001 tccaaaatct atcttcaaaa gaatgttgca tctttttatc ctttcacaca aacatttctc
    21061 tttatacaga tccaagtgat aaccagatga taattggaca ctttttggtt atttgtgtat
    21121 tttttgaaaa attttttaaa aaaattttat atcctcttta atagaagttt atgaatttgt
    21181 atatatgtat tccatcctat gttatgtata ataacataaa attgtgctaa atatcataat
    21241 ttatattatt ttccgagaaa ttatagccat tgacattacc taggaattgc gttataacat
    21301 acatgtaagc ggtacccaaa atggtgccaa gtcaaaattt ttaaggagga aaatacatgt
    21361 cttctcatcc aattcaggtc ttctcagaaa ttgggaaact gaaaaaagtt atgttgcacc
    21421 gtccaggcaa ggagttagaa aacttgttgc cggactatct tgaaaggctt ctttttgatg
    21481 atattccttt cttggaagat gctcaaaaag aacatgatgc atttgcccaa gctcttcgcg
    21541 atgaaggaat tgaggttctc tacctagaac aactcgctgc tgaatcattg acctctccag
    21601 aaatccgcga tcaatttatc gaggaatact tagacgaagc caacatccgt gatcgtcaaa
    21661 ctaaggttgc tattcgtgaa ttgcttcacg gcatcaagga caaccaagaa ttggttgaaa
    21721 aaacaatggc tgggattcaa aaagttgaat tgccagaaat tcctgacgaa gctaaagatc
    21781 taactgactt agttgaatca gagtatccat ttgcaattga cccgatgcca aacctctatt
    21841 tcactcgcga cccatttgca acaattggaa acgccgtatc gcttaaccac atgtttgcag
    21901 acactcgtaa ccgtgaaaca ctctacggta agtatatctt caaataccac ccaatctatg
    21961 gcggaaaagt ggatttggtc tacaaccgtg aagaagatac gcgtatcgaa ggtggagacg
    22021 agctagttct ttctaaagac gtccttgcag taggtatctc tcaacgtaca gacgcagctt
    22081 ctatcgaaaa acttttggtc aacatcttca agaaaaatgt tggcttcaag aaagttttgg
    22141 cctttgaatt tgctaacaac cgtaaattca tgcacttgga tactgtcttc actatggtag
    22201 actatgacaa gttcactatt cacccagaaa tcgaaggcga ccttcacgtt tactcagtta
    22261 cttacgaaaa cgaaaaactt aaaatcgttg aagagaaagg tgacttagct gaacttcttg
    22321 ctcaaaacct tggtgtagaa aaagttcatt tgattcgttg cggtggtggc aatatcgtag
    22381 cagctgcgcg tgaacaatgg aacgacggtt ctaacacttt gaccatcgca cctggtgtgg
    22441 tagttgttta tgaccgcaat accgtgacca ataagatttt ggaagaatac gggcttcgct
    22501 tgattaagat tcgcggaagt gaattggttc ggggccgtgg tggacctcgt tgtatgtcta
    22561 tgccatttga acgtgaagaa gtgtaatcgc tgttcggtat tcgtcaatag aaaatgtaaa
    22621 aaatagaaag aggaaataat aaaatgacaa attcagtatt ccaaggacgc agcttcttag
    22681 cagaaaaaga ctttacccgt gcagagttag aataccttat tggtctttca gctcacttga
    22741 aagatttgaa aaaacgcaat attcaacacc actaccttgc tggcaagaat atcgctctcc
    22801 tatttgaaaa aacatctact cgtactcgtg cagcctttac aactgcggct atcgaccttg
    22861 gtgctcaccc agaatacctc ggagcaaatg atattcagtt gggtaaaaaa gaatctactg
    22921 aagatactgc taaagtattg ggacgtatgt ttgacgggat tgaattccgc ggattcagcc
    22981 aacgtatggt tgaagaattg gcagaattct caggcgttcc agtatggaac ggtctaactg
    23041 acgaatggca cccaactcaa atgctcgctg actacttgac tgttcaagaa aacttcggtc
    23101 gcttggaagg cttgacattg gtatactgtg gtgatggacg taacaacgtt gccaacagct
    23161 tgctcgtaac aggtgctatc cttggtgtca atgttcacat cttctcacca aaagaactct
    23221 tcccagaaaa agaaatcgtt gaattggcag aaggatttgc taaagaaagt ggcgcacatg
    23281 ttctcatcac tgaagatgct gatgaagcag ttaaagatgc agacgttctt tacacagacg
    23341 tttgggtatc aatgggtgaa gaagacaaat tcgcagaacg tgtagctctt cttaaacctt
    23401 accaagtcaa tatggactta gttaaaaaag caggcaatga aaacttgatc ttcctacact
    23461 gcttgccagc attccacgat actcacactg tttatggtaa agacgttgct gaaaaatttg
    23521 gtgtagaaga aatggaagta acagacgaag tcttccgcag caagtacgct cgccacttcg
    23581 atcaagcaga aaaccgtatg cacactatca aagctgttat ggctgctaca cttggtaacc
    23641 tttatattcc taaagtataa ttttagataa taaaccgtct accaacagct atgagggctg
    23701 cgactaatag ctttagtccg gtcctctttt atgtaatggt aatctattat ttcttataaa
    23761 atatgtgaaa aatcattaaa ttgaaatcta aacgcattct attgagtgtg ataaaggaga
    23821 atttatggca aatcgtaaaa ttgtagtagc tttgggagga aatgcgattc tttcttctga
    23881 cccatcagca aaggctcaac aagaagcttt agttgaaaca gctaagcatc ttgtaaaatt
    23941 gattaaaaat ggagatgatc tgattatcac tcacggtaat ggacctcaag ttgggaatct
    24001 cttgctccaa catttggcat cagactctga aaagaaccct gccttcccac tcgactcact
    24061 tgtcgctatg acagaaggta gcatcggttt ctggttgaaa aatgctttgc aaaatgctct
    24121 cttggatgaa ggcatcgaaa aaaatgttgc ctctgttgta acgcaagttg tcgtagataa
    24181 aaatgatcca gcttttgtta acttgagtaa accaatcggt cctttctatt cagaagaaga
    24241 agcaaaagca gaagccgaaa agagcggagc gactttcaag gaagatgctg gccgtggctg
    24301 gcgtaaggtc gttgcctcac caaaacctgt tgacatcaaa gaaattgaaa ccatccgtac
    24361 tcttttaaat aatggtcaag tcgtcgtagc tgcaggtggt ggcggtattc ccgttgtcaa
    24421 agaaaacaat ggacatttga ctggtgtcga agcggttatt gataaagact tcgcttccca
    24481 acgtttggca gaattggttg atgcagacct cttcatcgtt ttgacaggtg tagattatgt
    24541 atttgttaac tacaacaagc caaaccagga aaaattggaa catgtgaatg ttgcccagct
    24601 ggaagaatat atcaaacaag atcagtttgc accaggtagc atgcttccaa aagtagaagc
    24661 agctatcgct tttgtcaatg gtcgtccaga aggaaaagca gttattactt cccttgaaaa
    24721 tctaggcgcc ttgattgaat ctgaaagcgg aacaattatt gaaaaaggat aagttgtttt
    24781 actaataaga tgtattctat ttctagtatc tttatatcaa attagaaatt attcttgaaa
    24841 acatgtacaa tatttcaaaa gatactagtt ttttagactt taatatggta aaacaaatat
    24901 agatggaaag cgttttctta aatgtttatt taagaaagta gttggttttt tacactttgt
    24961 tagacatcag gaggaaaaac aaatgagtga aaaagctaaa aaagggttta agatgccttc
    25021 atcttacacc gtattattga taatcattgc tattatggca gtgctaactt ggtttatccc
    25081 tgcgggggcc tttatagaag gtatttacga gactcagcct caaaatccac aagggatttg
    25141 ggatgtcctc atggcaccga ttcgggctat gctaggtact catccagagg aaggttcgct
    25201 cattaaagaa acgagcgcag cgattgatgt agccttcttc atccttatgg ttggtggttt
    25261 ccttggcatt gtcaacaaaa ctggtgctct tgacgtaggg attgcctcta tcgtgaagaa
    25321 gtataagggc cgcgaaaaaa tgttaatttt ggtattgatg cctttgtttg ccctcggtgg
    25381 tacaacttat ggtatgggtg aagaaacaat ggccttctat ccactccttg tgccagttat
    25441 gatggccgtt ggttttgata gcctgactgg tgttgcaatt attttgctcg gttctcaaat
    25501 cggctgtttg gcatctactc tgaatccatt tgcgacaggt attgcttcag cgactgcggg
    25561 agttggtaca ggggacggta tcgtacttcg tctgatcttc tgggttacct tgactgctct
    25621 tagtacttgg tttgtttacc gttatgcgga taagattcaa aaagatccga ctaagtcact
    25681 ggtttatagt actcgcaaag aagatttgaa acactttaac gtagaagaat cttcatctgt
    25741 agaatctaca cttagcagca aacaaaaatc agttctcttc ttatttgtgt tgacattcat
    25801 cttgatggta ttgagcttca ttccatggac agaccttggc gttaccattt ttgatgactt
    25861 taatgcttgg ttgactggtc ttccagttat tggtaatatt gtcggttcat ctacttctgc
    25921 actaggtact tggtacttcc cagaaggcgc catgctcttt gcctttatgg gtatcctgat
    25981 tggtgttatt tatggtctta aagaagataa gattatctct tccttcatga atggtgctgc
    26041 tgacttgctc agtgttgcct tgatcgtagc gattgctcgt ggtattcaag ttatcatgaa
    26101 cgacggtatg attaccgata caatcctcaa ctggggtaaa gaaggcttga gcggtctatc
    26161 ttcacaagtc tttatcgttg taacttatat cttctatcta cctatgtcat tcttgatccc
    26221 atcttcatct ggtcttgcca gcgcaactat gggtatcatg gctccacttg gagaatttgt
    26281 aaatgtccgt cctagcttga ttatcactgc ttaccaatct gcttcaggtg tcttgaactt
    26341 gattgcacca acatctggta ttgtgatggg agctcttgca cttggacgta tcaacattgg
    26401 tacttggtgg aaattcatgg gcaaactcgt agtcgctatt attgtagtga ccatcgccct
    26461 tcttctcctt ggaaccttcc ttccattcct ataaaatagt gagtgaggtg attccatgaa
    26521 aatagatata acaaatcaag ttaaagatga atttcttata tcattaaaaa ccttgatttc
    26581 ctatccttca gtactcaatg aaggagaaaa tggaacacct tttggacaag caatccaaga
    26641 tgtcctagaa aaaactttag agatttgtcg agacataggt ttcactacct atcttgaccc
    26701 taaaggttat tacggatatg cagaaatcgg tcagggagca gagcttctgg ccattctctg
    26761 tcatttggat gttgttccat caggtgatga agcagattgg cagacaccgc catttgaagc
    26821 aactatcaaa gacggctggg tattcggacg tggtgtccaa gatgataaag gcccttcgct
    26881 cgcagctctc tatgcagtaa aaagcttgct ggaccaaggt attcagttca aaaagcgcgt
    26941 acgctttatc tttggtaccg atgaggaaac cctctggcgc tgcatggcac gctacaatac
    27001 catcgaagaa caggccagta tgggctttgc acctgactca tcttttcctc tgacctatgc
    27061 tgaaaaaggg cttctacagg tcaaacttca tggccctgga tcggatcaac tagagcttga
    27121 agtaggaggc gcctttaacg ttgtaccaga caaggccaac taccaaggtc tcctctatga
    27181 acaggtttgt aacggtatca aagaagctgg ttatgattac caaaccactg aacaaaccgt
    27241 aacggttctc ggagtgccaa agcatgctaa ggatgctagt caaggtatca atgctgtcat
    27301 ccgactagct accattcttg ctcctctcca agaacaccct gctctcagtt ttcttgcaac
    27361 acaagcaggt caagacggca caggaagaca aatctttggt gatatagcag atgaaccttc
    27421 tggtcaccta tcctttaatg tcgcaggtct catgatcaat catgaacgtt ctgaaatccg
    27481 tattgacatt cggactcctg tcttagctga caaggaagaa ctagtaaagt tgcttacaag
    27541 atgtgcacaa aactaccaac tccgctacga agagtttgac tatctagcgc ctctatacgt
    27601 cgcaaaagac agtaaactcg ttagcacact gatgcaaatc taccaagaaa agactggcga
    27661 taacagtcct gctatttcat ccggtggtgc cacttttgct cgcaccatgc caaattgtgt
    27721 agccttcggc gccttattcc caggagcgaa gcagacagaa catcaggcaa atgaatgtgc
    27781 cgttctagaa gatttgtacc gtgctatgga tatttatgcc gaagccgtct atcgacttgc
    27841 aacttaatca ggcaactgtt tctaccaaaa aaaatcgacc gattaatgaa ctgcacccca
    27901 aaagttagac agaataaatc taacttttgg ggtgttttat tatgaaattg aattatgaag
    27961 ataaagttca gatctatgaa ctaagaaagc aaggacaaag cttcaaacag ctttcaaaaa
    28021 gatttgtgtg gatgtttctg gtctaaagtc atctgaatct ttgagatgag ctttataaat
    28081 cgcttttttc agtttttgca ctggtgtttc gataaactca aactttttag ccgtggtatt
    28141 gcctgatttt atagtatatt gaaactagaa tagtacacct ctacttctaa aacattttta
    28201 gaaatcgatt tgactgtcct gatcgatttg tcctgttctt atttcatttt actatatttg
    28261 agccacttcg tctttaacgg ctttattcat aagctcttgt aatttttctt tactatcaat
    28321 tacttctgat tttccgttgt aatttattgt aataggtttt aacttaccta atttctcgac
    28381 acgctcatta atttgatctt ttttgaaggc tgcatatgtt tttcctaaga ttttttcgaa
    28441 aatatattta tcagatagcg gtttgccttc ttcttcagct tggtttttgt attgatttga
    28501 aacataagga acaaatcctt catagtaacc taatgctccc ataagttcaa aagcttgttt
    28561 tctaattcaa accattgcaa ctcagatttc agcttttcag ataaatcctg ctcatccaaa
    28621 taatgactta aaattagtgc tgaactcgtt tctgtatcct gtacaggctg atcacccata
    28681 ccagcaaaaa ataaactcgt tcctagcaag accgaacaag ctcctattgc atatttcctc
    28741 aaagaaaaac gctgctttct ctcaaattga aattctttca tcccatctcc catcattcat
    28801 tattactgta tattttgtat atcagaaata gtttgtattc acaaatcttt ctatttattc
    28861 ccttatcatt cctaattaag ggagataaca tacaataatt tttagttaaa tgtatagcga
    28921 tgttttttgt ttttcttaat aaacgcaata caaaaagagc ctgttgccaa gctctttgta
    28981 ctcaatgaaa atcaaagagc aaactaggaa actagccaca ggttgctcaa aacaccgttt
    29041 tgaggtagta gataa
//
LOCUS       DAVBDG010000023        28544 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBDG010000023 DAVBDG010000000
VERSION     DAVBDG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28544
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.023"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            179..391
                     /locus_tag="VQ526_000985"
                     /pseudo
     CDS             179..391
                     /locus_tag="VQ526_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            291..737
                     /locus_tag="VQ526_000986"
     CDS             291..737
                     /locus_tag="VQ526_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2554722.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(875..2179)
                     /gene="eno"
                     /locus_tag="VQ526_000987"
     CDS             complement(875..2179)
                     /gene="eno"
                     /locus_tag="VQ526_000987"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2554723.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2343..2789
                     /locus_tag="VQ526_000988"
     CDS             2343..2789
                     /locus_tag="VQ526_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET2554724.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPLGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2786..3901)
                     /locus_tag="VQ526_000989"
     CDS             complement(2786..3901)
                     /locus_tag="VQ526_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2554725.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPSGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASDQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3910..4555)
                     /gene="serB"
                     /locus_tag="VQ526_000990"
                     /pseudo
     CDS             complement(3910..4555)
                     /gene="serB"
                     /locus_tag="VQ526_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            complement(4614..6047)
                     /gene="glgA"
                     /locus_tag="VQ526_000991"
     CDS             complement(4614..6047)
                     /gene="glgA"
                     /locus_tag="VQ526_000991"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2554726.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGSQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6044..7183)
                     /gene="glgD"
                     /locus_tag="VQ526_000992"
     CDS             complement(6044..7183)
                     /gene="glgD"
                     /locus_tag="VQ526_000992"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2554727.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7173..8315)
                     /locus_tag="VQ526_000993"
     CDS             complement(7173..8315)
                     /locus_tag="VQ526_000993"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2554728.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8305..10233)
                     /gene="glgB"
                     /locus_tag="VQ526_000994"
     CDS             complement(8305..10233)
                     /gene="glgB"
                     /locus_tag="VQ526_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2554729.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGSLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            complement(10716..12140)
                     /locus_tag="VQ526_000995"
     CDS             complement(10716..12140)
                     /locus_tag="VQ526_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2554730.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(12684..14963)
                     /gene="pulA"
                     /locus_tag="VQ526_000996"
     CDS             complement(12684..14963)
                     /gene="pulA"
                     /locus_tag="VQ526_000996"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2554731.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(15075..17033)
                     /gene="ligA"
                     /locus_tag="VQ526_000997"
     CDS             complement(15075..17033)
                     /gene="ligA"
                     /locus_tag="VQ526_000997"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2554732.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(17126..18304)
                     /locus_tag="VQ526_000998"
     CDS             complement(17126..18304)
                     /locus_tag="VQ526_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2554733.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQTIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(18384..19247)
                     /locus_tag="VQ526_000999"
     CDS             complement(18384..19247)
                     /locus_tag="VQ526_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2554734.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGLALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(19250..21151)
                     /locus_tag="VQ526_001000"
     CDS             complement(19250..21151)
                     /locus_tag="VQ526_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2554735.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRTILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEQTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKVTAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
     gene            complement(21578..21853)
                     /locus_tag="VQ526_001001"
     CDS             complement(21578..21853)
                     /locus_tag="VQ526_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2554736.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(21960..22799)
                     /locus_tag="VQ526_001002"
     CDS             complement(21960..22799)
                     /locus_tag="VQ526_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2554737.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILCHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            22941..23789
                     /locus_tag="VQ526_001003"
     CDS             22941..23789
                     /locus_tag="VQ526_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2554738.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(23882..24799)
                     /locus_tag="VQ526_001004"
     CDS             complement(23882..24799)
                     /locus_tag="VQ526_001004"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2554739.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRIIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            25236..25441
                     /locus_tag="VQ526_001005"
                     /pseudo
     CDS             25236..25441
                     /locus_tag="VQ526_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(25752..26045)
                     /gene="rpmA"
                     /locus_tag="VQ526_001006"
     CDS             complement(25752..26045)
                     /gene="rpmA"
                     /locus_tag="VQ526_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2554740.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(26062..26406)
                     /locus_tag="VQ526_001007"
     CDS             complement(26062..26406)
                     /locus_tag="VQ526_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2554741.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(26422..26736)
                     /gene="rplU"
                     /locus_tag="VQ526_001008"
     CDS             complement(26422..26736)
                     /gene="rplU"
                     /locus_tag="VQ526_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2554742.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(26772..26859)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(26954..27553)
                     /locus_tag="VQ526_001009"
     CDS             complement(26954..27553)
                     /locus_tag="VQ526_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2554743.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLF
                     SSFVLGVVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            28023..28322
                     /locus_tag="VQ526_001010"
     CDS             28023..28322
                     /locus_tag="VQ526_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2554744.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         8756 a         6121 c         4961 g         8706 t
ORIGIN      
        1 acgatgacga gcgacttttt tgaagcttgg tttcagaaat ttctcttacc aacattaacc
       61 gcaccattgg ttattatatt aaaacgaagt aagaatagga caaatcgatc aggacagtaa
      121 aatctaattc taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatctactat
      181 attaatggac aatgcaagat tccatagaat ggggaagcta gaactcttgt gtgaagagtt
      241 tgggcataaa cttttacctc ttcctcccta ctcgcctgag tacaatctta ttgagaaaac
      301 atgggctcat atcaaaaagc acctcaaaag ggtattacca agttgcaata ccttttatga
      361 ggctcttttg tcttgttctt gtttcaattg actatataaa ttgtctaagc gaaacaaccg
      421 ataagaattg gcacaaaagc gaccgtattt ttgttaccaa tacaggaaaa ccagttcata
      481 gttctatctt gagcaagtct ctccagcgag caaacgaacg ccttaaaaaa ccaattccca
      541 aacatctgtc ccctcacatc ttcagacaca ccactattag catcttatca gaaaataaaa
      601 ttcctttaaa aacaatcacg gacagggttg gtcattccga ctctgaagtc actacttcca
      661 tctacaccca cgtcacaaag aacatgaaag atgaagcaat caatgtactg gataaagtta
      721 tgaaaaagat tttttaaaaa gttttgtccc ttttttgtcc tctaaataca aaaatagccc
      781 ttcggataaa atccgagggg ctagaaacgt tgttaaatca acggccgaac ttttgaattt
      841 catggttcgg gataaaatag ttcactgaac tattttattt tttaaggttg tagaatgatt
      901 tcaatccacg atattcagct acttcaccaa gttggtcttc gatacgaagc aattggttgt
      961 atttagcgat gcggtctgta cgtgaaagtg aaccagtctt gatttgtcct gcgttagttg
     1021 caactgcaat atcagcgatt gttgaatctt cagtttcacc tgaacggtgt gatacaacag
     1081 cagtgtaacc agcttcttta gccatttcga tagcttcaaa agtttcagta agagtaccga
     1141 tttggttaac tttgataagg attgagttag cagcaccttc ttggatacca cgtgcaaggt
     1201 agtcagtgtt tgttacgaag aagtcgtcac caacaagttg tactttctta ccaagacgtt
     1261 cagtaagagc tttccaacca tcccagtcgt tttcatccat accatcttca atagtgatga
     1321 ttgggtattt gttaaccaat tcttcaaggt agtcgatttg ttctgcagat gtacgaacag
     1381 cagcaccttc accttcaaat ttagtgtagt cgtaaacttt acgttcttta tcgtagaatt
     1441 ctgatgaagc acagtcaaat ccgataaata cgtctttacc tggtacatat ccagcagctt
     1501 caatcgcagc aaggatagtt tcaacaccat cttcagttcc ttcgaaacga ggagcgaatc
     1561 caccttcgtc acctacggca gtttccaaac cacgtgattt aaggattttc ttaagagcgt
     1621 ggaagatttc agcaccgtaa cgaagggctt ctttaaatgt tggcgcacca actggcaaga
     1681 tcatgaactc ttggaaagcg attggagcgt cagagtgaga accaccgttg atgatgttca
     1741 tcattggagt tggaagaact ttagtgttga atccaccaag gtagctgtaa agtgggattt
     1801 caaggtagtc agcagcagca cgagctacag cgatagacac accgaggatt gcattcgcac
     1861 ccaatttacc tttgttagga gtaccgtcaa gtgcgatcat agcacggtca atagcttgtt
     1921 gatcacgtac atcgtagcca atgatagctt cagcaatgat gttgtttacg ttgtcaacag
     1981 ctttttgtgt accaagacca ccgtaacgag atttgtcacc gtcgcgaagt tcaactgctt
     2041 cgtgttcacc agtagaagct cctgatggaa ccataccacg tccgaaagca cctgattcag
     2101 tgtaaacttc tacttcaagt gttgggttac cgcgtgagtc taggacttcg cgagcgtaaa
     2161 catcagtaat aattgacatt ttttactctc cttatgagtt aaatttttta cacctctata
     2221 ataccttaaa acccctcctt tttcaagaaa aaacgttatc tttgtgcaac ttttccttaa
     2281 ctttataaag taatcgcttt cttttgtctg ttttattcta acttttatga tatactgttt
     2341 tcatgacaga tttatcaaaa caattacttg aaaaagctca tggtgggtta aaaataaatc
     2401 cggatgagca aagacgctat cttggtactt ttgaggaaag agttcttgga tatgtagata
     2461 ttgacacagc aaatagccct cagttagaaa aaggcttttt atttatttta gaaaaccttc
     2521 aggaaaaagc agagccacta tttgtgaaga tttcaccaac tatcgaattt gataagcaag
     2581 ttttctactt aaaagaagca aaagaaactg atagtcaagc caccatagta tctgaagagc
     2641 atattacttc tcctttgggc ctggttattc atagcaatgc accagttcaa gtagaagaaa
     2701 aagaccttcg acttgctttt ccaaaacttt gggaagttaa aaaggaagaa ccagccaaaa
     2761 catccttatg gaagaaatgg tttagctaaa tcttgcacat atttaataag tgcccaatat
     2821 tggcagccgt gcgctccaga tagagactgg catttttcaa actatcttct aaaggttcac
     2881 ttttctccaa aatagaaaag acagcttgga tattttcaaa tggtagggga ggtaaatctt
     2941 cagcaagact accgcaaata gcaataacag gaactccaac aggggttctt tttgcaacac
     3001 ctataggcgc tttcccagca aagctttgac tatcaagtct tccttctcca acaacaacca
     3061 agtcagcatc tgaaactttc ttatcaaagt tgattaagtc caagcaggta tcaattccag
     3121 acacgatact tgcctgagca aaggcacaca aaccaccagc aaggcctcca cctgctcctg
     3181 ctcctttaat ttctaatgtt gcaggtgaga atttttcata aaaatcttgg atcgcctgat
     3241 ctacgactgc aaacatagtc ggatgtagac ctttttgatt gccaaaagtg taagtcgcac
     3301 cttgatgacc acataaggga ctcacgacat ctgctagaat atgaatttga acaccttcag
     3361 gaattttata gcaattttct gttgaaacag aagctaagtt taataaggat tgatcggaag
     3421 caggcaagac atttccatcc ctatcataaa attgataacc taaaccagca gcaatcccca
     3481 gtcctccatc attactggcc gtgccaccaa caccgatata aatatcttta atccctttag
     3541 aaatgagatg gagaatcaac tctccaatac cacaagtttg gatttgaagt ggatttcgtt
     3601 tctctagcgg aatttttcca agaccaacca agtcagctac ttcaaatagt gccagttccc
     3661 ctttttgaaa atagcgcatg gcttcttttt gtccagaagg gtctgtcact tggatccatt
     3721 tttcttttag gtcaagagaa tgtcggatag catctacagt accttctccc ccatcaccaa
     3781 cagggcagag gagacattct acatctgcta tcgattgttg gaagcctctt tttattgctt
     3841 cagctacctg ttgagctgtc aagctttcct taaacgaatc cggtgcaatt acaatcttca
     3901 tattttccct cattctaaac agtcaatcaa aggaagaact tctaaaaaat ccctcttgtc
     3961 aacatgatgt ggtatttctt ttttgagcat ttctttggaa caaaaggcga ttcctcactc
     4021 cgccgacttc aacattaata gattattgac cccatcaccg attgccaccg ttctttcttt
     4081 agaaagtttt agtttctttc tccatttttc cagagtttct tttttgacct ggggacttat
     4141 aatttgtcca actaattttc ctgttaaaag accttctttg acttcaagct ggttggcagt
     4201 gaaataggca ataccaaggg attttgctaa tctctccaac tattggtgta aatccaccag
     4261 acaccagacc aactaggatg ccattctttt ggagaataga gatgaactct gggacattta
     4321 gcgatagatg aattgagttg aagacgttat caaagaccaa aataggaaga ccttccaaca
     4381 aggacactct ttttcttaaa ctgctttcaa agaccaactc tcctcgcatt gctcgacttg
     4441 taatctgcga aatttccgcc tcatgacctg cctctctccc taaaagatca atcacttctt
     4501 ctaggattaa ggttccatct acattcaaaa cacacaagcc ttttacttga gacatcagtt
     4561 ctcctctcta aacagcctaa aaatcgtatg aagtcatcat acgattttat ctattaatta
     4621 actaaactat ggtacaagtc aaggtatgac ttgcaggctg tatcccatga gaagtcactc
     4681 tccatagctt gtttttgtag gtttctccaa atgtctggat ggtttctata caagtccaat
     4741 gctgtttgga aagtccaatt taaccaataa ggagatagat tgtcaaagct aaagccagta
     4801 ccgcttcctt cgattggatt gaaagcgcga actgtatctc gcaagcctcc aacttcatgg
     4861 accaatggca aggttccata acgcatagcc atcatttgag acaagccaca cggttcaaaa
     4921 cgacttggca tgaggaagag gtcacaagca gcgtagattt cctgagcaag tttgacatca
     4981 aaagtgatat ttgttgatag cttgtctggg taaatctgag caaaccatga gaaagctcct
     5041 tcaaaggctg gatcgccagt tcccaaaaga acaatctgaa catcttcttg caagatatgg
     5101 tgaagacttt cgaccaccac atcaaaacct ttttgacgtg tcaaacgaga aacaattccc
     5161 accagtggaa cgtctgctct aacaggcaag ccaactcttt cttgcaattt tgccttattt
     5221 ttggctttcc cagacaaatc ttcctgattg aaatgatagt ctaaaagagc atccgtctga
     5281 ggattataaa gatcagcatc aatcccattc acgataccag atactttacc agactccatt
     5341 ttaagaatct gatccaaatt acatccaaac tgactagtca taatttcatg agcatagcta
     5401 ggtgaaacag ttgaaacacg gttcgcatag agaatacctg ccttcatcca gttcagacag
     5461 ttgttccatc gaagggtgcc atcagcgtaa cgttcaaagc caactccaaa caaatcaccc
     5521 aacattcctt ctgaaaattg tccttggaat tctaaattat gaatggttaa aactgtttca
     5581 atgtcctcat aggcttgaat ccaacggtat ttttccttca acaagaaagg aatcatagct
     5641 gtatggtagt catgaacatg gagaagatca ggaataaagt caatcctttc catagcctca
     5701 atggcagcca gttggaaaaa ggcaaagcgt tctccgtcat caaaatcacc gtaaacatga
     5761 ccacggaaga aataatattg attgtcaata aagtagaagg ttacaccatt taatactgtt
     5821 ttcttaattc cacaatactg tctgcgccaa ccaacgctca cctcaaaatg aagcacatct
     5881 tcaatctgac ttccaaattt agcctctacc atatcatagt agggtaaaat cactgcaact
     5941 tcgtgcccag cttttaccag tgattttgga agagcgccaa tgacgtctcc caaaccacct
     6001 gtttttgaaa agggtgcacc ctctgctgct acaaataaaa ttttcatgaa tgaatatcct
     6061 ctgttacttt agcacctttc ttaaccacaa ctggatgttc tgcagttcct cgaatcacaa
     6121 caccaggctc aacttcaacc cctttgtcca agatagcata ttcgacctga gccccttctc
     6181 caataacaac acgagggaag agcaggctat ctttaaccaa gctatcctta tggacatgaa
     6241 tattacgtga tagaacagaa ttagctactt gaccttcaat aatactacca gaggcaaact
     6301 gagaagtgct taccttagat gtattagcat agtaagttgg ctcttcgttt ttgacctttg
     6361 tataaatctt ttggtttggt gagaaaagag aatagaattt ttgtgattca agcatatcga
     6421 tattcgcttg ataataagat ttaacagagt gaatattggc tagatagccc gtgtactcgt
     6481 aggcgaaagc tccctctttt acagccaaat cccgtaaaac atagcgcaat ttctctggat
     6541 gttctttttt agcttcttct tccaagtgtt caatcaacca aggtgtatca acgacaaaga
     6601 tatctgtaga catattgaac gtttcagctg ttgacttgct atcaaagagt ttatgagaaa
     6661 gaacatggtc tgtttcatct acatccaaga ttgcatttac ttctgaaata tctttcttag
     6721 ctagtttttt ataaactaca gtgataggct cttttgttgt actatgtagg tggaaaactt
     6781 ggttcaaatc aatgttaata agaacatcgc agttgagggc aaccgtttgg tttgagccag
     6841 aacgtttcaa ataagtaaga agctgttggt agtattcttt tccaactgta ctactttcta
     6901 cacgggtatt gtaaattcct agatagtaat ggctaagaag ggttgataag ccccactcgc
     6961 gtcctgaacg aatatggtca aatactgagc tgatattatc ctgctggaaa ataccaaaga
     7021 cactacgaac acctgcatta gcaaggcttg aaagtgggaa gtcaatcaaa cgatatttcc
     7081 caccaaatgg caaacttgct actggacggt ggtccgtcaa tgtcgacata ttgtgaaaac
     7141 caactgtatt tcctaaaatg gcagaatatt tatcaatctt catctgttgc tacccccact
     7201 acttcattat atcctacaac ttgtacttca tctgttccat caatttcgac accgtcagaa
     7261 ataatcgcac cttcaccaat aatggcacgt ttaatcttag ctccttgacc aatgatagct
     7321 ccactcatga taactgaatc aaggacttcc gctccttcgc gaacttgcgc gcctgttgaa
     7381 aggatagaat gtttaacagt tccatcaacg aaacatccgt ctacaactaa tgagtcttcc
     7441 acatgagcat ttgccccgag gaagtttggt ggtgaaatca agtttcttga gtaaatcttc
     7501 cattgacggt tacgactatc caaggcattt tctggagaaa tatactccat gttcgcttcc
     7561 caaagtgact caatagtacc aacatctttc caataaccac taaattcata agcataaaca
     7621 ctttcacctg actcaaggta atttggaatg acatttttac caaagtctga catgccaacc
     7681 ttgctctttt cagcagcgac taacatatta cgaaggcgtt gccaatcaaa aatgtagatt
     7741 cccatagaag cttttgtaga tttaggttga gctggttttt cttcaaattc aacaatacga
     7801 ttgttagcat ctgtgttcat gataccaaaa cggcttgctt ctttaagagg gacgtctaaa
     7861 actgctactg tcaagctggc attattatcc ttatgagact ggagcatatc atcatagtcc
     7921 attttgtaga tgtggtcccc agacaaaatc aagacatact caggattgac actgtcgata
     7981 tagtcgatat tttggtaaat agcgtgacta gtcccctcaa accaacgatt tccttcactt
     8041 gcagaataag gttgaagaat agagacacct gaattaatac cgtctagtcc ccagcttgaa
     8101 ccattcccaa tatggttgtt gagagcaagt ggttgatact gtgtaacgac cccaacatta
     8161 tgaatccctg agttggcaca gtttgatagg gcaaagtcaa tgatacggta gcgcccacca
     8221 aattgcacag ctggttttgc gatgctttga gtgagtttac cgagacgagt tccttgccca
     8281 ccagcaagaa tcaaagctaa catttcattt ttcattttct actccttttt ggtttttatt
     8341 tgtgacggtt ttagtagatt tcaagcgacg tttgattttc catacacttg ctcccatagc
     8401 cggtagagta aaggttaagg tctgctcata atctttccat agtccttctt gcgtttgaac
     8461 agtttgatta tgttctttcc aaacgcctcc ccactcttcc aactcagtat tccatacttc
     8521 ttcgtaaatt cctgcaacgg gtagtccgat tgtaaaatct ttccgctcaa caggtaccat
     8581 attaaagata cagactaaca tttctccctt tttaccctta cgaataaagg aaagaacact
     8641 ctggtctcga ttatccgcat caatgatttc aataccatca tagctggtat caatttccca
     8701 cagacagcga tgatctttgt aaaactggtt tagctgagaa gcgaaatact tcatcttagc
     8761 attcattggg tcttctaggt tagaccattc caactgttct tcagatttcc attctaggaa
     8821 ttgaccgtat tcgctaccca tgaagagcaa tttcttacca gggtgacaaa tttggtacgt
     8881 atagagattg cgcaagcctg cgaattgatt gtaacgatct ccccacatct tatgcatcat
     8941 actcttcttg ccatgaacca cttcatcgtg cgagaatggc aagagataat tctccttgaa
     9001 aacatacata aagctgaaag tcaccaggtt aaagtcatat ttacgataaa tcggatcttc
     9061 ttcgtagaaa cggaggatat cattcatcca gcccatgttc catttgtagt caaatcctag
     9121 actaccaatc tctttcattc ccgtaatctt ggtcgcagac gaactttctt ctgcaatcat
     9181 catcacatct ggatattcta acttaataac ttcattcaag cgttgaagaa aataataacc
     9241 ttcatagttg agatttccgc catctttatt aggtgtccat ggagcattat catagtccaa
     9301 atagagcatg ttgctaacag catccacacg aataccatcc aaatgataga catcaatcca
     9361 atgcttaatg caagaaatta agaaggactg gacttcattt tttccaaggt caaaattaag
     9421 ggcaccccaa ccatggttat gagccttatt atggtcttgg tattcaaaag tcggtgtccc
     9481 atcataatag gctaaggcat catcgttgat ggtaaagtga cctggtaccc agtccacaat
     9541 aaccccaata ttatgggtat gacactcctc gacaaaatct tgaaactcct ctggtctgcc
     9601 ataagcatgc tctaaagcga agtaacccat aagctgatac ccccaactca agcccaaagg
     9661 atgggacatc aagggcataa actcaatatg agtatagttc atttcaacga gataaggaat
     9721 gagttcatcc ttgagctggg caaaactata aggactgcca tcagaatttc ttttccatga
     9781 tccagcgtga acttcataaa tattgacagg acgctcttca aagccccaac gttttcttcg
     9841 tgccagccaa agtccatcct tccatttctt ctcaggaagc tctgttaaga ttgcccctgt
     9901 tcctggacga gcctcatacc tgacagcaaa agggtcaatc ttcatcagtt gatgaccatt
     9961 ttgacgtgtg acatgatatt tgtaaatatg cccttcttga gccatattgg taaagacttc
    10021 ccagacccca aaatcatttc ttaccattgg aatctgattt tcaatccagt tggtaaaatc
    10081 accaaccaag tgaacagcct gagcattagg tgcccaaaca cggaaggtat agccatgctc
    10141 tccatttagt tcttccctat gtgctcctag ataatgttgg agataaaaat tttcacccgt
    10201 cataaaggtt tttaatgctt ctctattatc catatactcc ccttctcctg taagcgtttt
    10261 ctatgttttt attatactac ctttttagag aagattcaag taaattacta tacttcttta
    10321 attattttga aaatctacaa caagttcact tactcgttca attgtaaatc aatatttttt
    10381 caaaaaattg cgaaaacgcc tttctttttc tactatagtg aaatgaaata aaacatgcgc
    10441 aagtcgatta aggaatttaa tctaatttct aacaatgtct tagaaatcaa agtgtactat
    10501 tttaacttca atgtactata tatcgtaaaa tgaaataata acaggacaaa tcgatcagga
    10561 cagtcaaatc gatttctaac aatgttttag aagtagagct gtactattct agtttcaata
    10621 tactataata ccaaaaagtt cgtatttttt ctataattat tattatttta tagcaaaaaa
    10681 ataaaaccag gaaaacaatt tcctgatttt acacgttatt tcacatcaaa aacaatggat
    10741 ttgacatttg tcatcgcttc aatgctatat ttaatccctt gcactccagc tccagaacct
    10801 ttgacaccaa ggaacgggaa attatctgga ccacgctggg ttttattatt aatgtggact
    10861 gtacctactt caagtttttc agcaatttca aaggcttttt tgaaatcatt tgtaaagact
    10921 gatgattgaa ggccgaattc agattcgttg gcaaaggcaa tagcttcctc tacactagcc
    10981 acacgaatga ttggtaaaac aggaccaaat ggctcttccc atgccacttt catatctttt
    11041 gtaacttggt caaaaagcac tggccagagg agattgccct cacgtttgat tggtgtaaga
    11101 gcctgagcct ctttttcttg tgcatcctca atcaagcccc aaatgaagtc ggctgaagca
    11161 ttgtcaataa caggtgtaat atcagcattg tcaaatggat caccaactgt taatttagaa
    11221 acttcttcct gaagcaaagt agctaattta tctgctacac tttcgagaac aatgacacgt
    11281 ttaatggccg tgcaacgttg tcctgagtag ctaaaggctc ccgcaacaat ttgcttggca
    11341 gcatgttcca aatctgcatc ttctagtaca agagctgcat ctttcccacc aagttccaac
    11401 atgataggac gcataccagc taaacgacca atacgttctc caataggagt tgaacctgta
    11461 aagttgatga agttgacttc tttgtgctca atgatataat ccccaatttc tgaaccacga
    11521 cctgtaatgg tgttgaaaac acctgccgga atccctgctt cttcaaatgc tttagccaac
    11581 aagagtccag aaatggaacc ttgtgttggt ggcttaaaca tgaccacatt ccctgcaatc
    11641 aaggcaggtg caattttaga agcagataaa ttaactggat aattaaaggg agcaatcgct
    11701 agcacgatac caactggttc acgacggaca acagccagtt tgtttttact tgctgcctca
    11761 aaaccaccac cttccattgc ttgtccagtg atacggagac cttcctcagc agcataacga
    11821 atcaagtctg ctgtacgcac tacttctcca attgctgctt taatcccttt tgctacttct
    11881 ttggcaagga tagtaccaat ttcttcctta tcgcgttcta aaatagctgc tgttttatgc
    11941 aaataagccg cacgttcaat tgctgataaa gctcgccatg ctggcagggc tgcacgcgca
    12001 gcttgcatag cctcatcagc ttcagtctga gtcatggctg gaactgtacc caattcttct
    12061 tgattgattg gtgaataaat cgtaatttct tgttcagatg atttccattt tccatttact
    12121 aaattctgat atcttgtcaa attcttgtcc tcctgaaaat gattaagata tattttacca
    12181 aattttcaga aaaccacaac cctttttgcg aaatttttga gattattttc acaagcttga
    12241 tttttcaaag tatactcaat aaaaataaaa aaatccacta cgtcaaggcg aggctaatgt
    12301 ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc tatagtaaaa taaaaaaact
    12361 gaacaatttg gtttgggaca gtcaaaccaa tttctcacaa tgttttagaa acacgggtgt
    12421 gctattccaa tttcaggcta ctataactgt catagattgc tgaaacaaag tctaggtaaa
    12481 agtcttcata ataaaaagac ctcctatcaa atgttcaaaa actttgatag gaggtcttgt
    12541 ttcgtgaaaa tatttatcaa attttctata caagtgagct gttagccagg ttctttctat
    12601 tctttcaatt tcaatgaatg gattttttac caatactcat aactgggaat ttttctgtgt
    12661 aaaaatagcg agatagatgg tatttataaa acactcaaga cagctagact aatatcattt
    12721 aaaacattat cttcttttga gcgactgttg gttaccaaca tagctaaatt tcctgcattt
    12781 tcaaattgat agggttctga tttagcattc acaaccacca agaggtgttc tttgccgtga
    12841 acttcataga taaggcagcc gctatgttca atcgccgaat gcacaaagac atgatggtaa
    12901 atttcatcat agctagagta agaaaaggca ccagtttttg tcttcaatcg gatgacttga
    12961 cggataaact caatactgtc ttgacgctca ttaatcaagt tccagttcac ttggttcaca
    13021 ctgtcaggag cattatagct attcatcgca cgctctctat catcatgggt caactcacca
    13081 ttttcaccag tcgcaaccag tttggtacga ccaaattctt gaccgatttc cataaaggcc
    13141 atcccctgca tgagcagatt catggctgtg gcagtttcga ccttgcgcat gatttgctct
    13201 gaactttggt ctggatgaag ggttgccaat aaatcgtgaa gattgtaatt gtcatgggct
    13261 tctacatagt taagcacctg atttggatgt gtatagcttc ctaattcacg acttcctagg
    13321 attgctttag ctagaattgg ctctgtcgca gcaccactga caaaacctga cttgatagca
    13381 ccataaactt ctcccccttt gacagcatcg cgctgattgt cattaaagaa accaatattt
    13441 ggcatctggt aggcattgtc cttcttggcc ttatcataag gggcaagacc tgttcccata
    13501 tcccatcctt ctccatagag gataatgttg gagtcgattt catccaagct ttgacgaatc
    13561 atctgcatgg tcttgacatc atgaatcccc atcaagtcaa aacggaagcc gtcaatatta
    13621 tattcctgca cccagtatag aagagaatca atcatatact tgcgaaacat ttcgtgttca
    13681 ctggctgttt catttccaac acccgttcca ttctggaagg taccatctgg attcatacga
    13741 taatagtaat cagggactgt tgtttggaat ggtgcatcaa caactgagaa ggtatggtta
    13801 tagactacat ccataatgac tccaataccc gcatcgtgat aagcttgaac catcaccttc
    13861 aaatcacgaa tgacctgagc tggatcatct ggattagttg aaaaactagt ttctggcgcg
    13921 ttatagtttt gtggatcata accccagttg taggttacat ttccatcctc atcgtattct
    13981 ttatgacggt ctgcaattgg ttgcaattga acataattgt agcccagctt cttgatgtaa
    14041 tcaaaagcag ttgactggcc gtattggtta actgttccag cctgagcagc acccaagaaa
    14101 gttcctcgaa gatgttcatc tacacccgat gtaggtgatt tagtcaaatc acgaatgtgc
    14161 atttcacaga taactgcctt acatggattt tccaagcgcc aagtagcctc cgaaccgtgc
    14221 ttaacctcga agttttcaac ttgcttttct acatggctca gaatagctga acgtttgcca
    14281 tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg tttggtgatg agggaattgg
    14341 acttgatact gataagcctt acctaccaaa tcttcctcaa catccaaact ccagacaccg
    14401 attgtattgt ccttatgatt ataagagtag ctattgcctc ttttcatctc aaaagtcttc
    14461 caaacgggtg catcattagc agctgattca taaacgacaa cttgcacttc tgtcgctgta
    14521 ggtgaccaga gggaaaaatg agcctgattg tcctctacac ggcaacccaa ttctccttgg
    14581 taaccccaat gatggtcaaa actagcactg ttaatggcct tatcaaaggc aaaaggattt
    14641 tgatttttat agaaaggact ggcaatagca ggattttcag agtaataaat cctatcatcg
    14701 ccttccaaaa tccagacttc tgttaatagg ggatagtgat taaaacggat agaatattct
    14761 ttactagttt gacctgtatg aaccacaaaa ttcaagcttt ctataacatg tgaacttggg
    14821 tgttcaaagc taaataaagc tccaaaataa tcttctttgt aggttagcaa atcaattcgt
    14881 tgatcctgac tttttacaaa ggagcaagtg tcatattcac cattcttgcg atggtaatga
    14941 atgcgcatag ggtagttata cattttttat ttttcctttt tactttgttt ctatttcact
    15001 aataaatttt tgtcaatctc gtctcaatta acagacatag tcatattctc taaactctgt
    15061 ttttaaacga tccattacaa actttctagc catgcctcat ctctgacctg gataccaagt
    15121 tcttgtgctt tttgcagttt acttccagcg tctgcaccta ccacgacgag gtcggtcttt
    15181 ttagaaacac tacctgtcac tttggcaccc agactttcga gtttactttt agcttctgag
    15241 cgcttgagtc gttccaattt tcctgtcaat accacggtca aacctgacaa ggccgcatcc
    15301 gctactaccg tctgtccttt atagtccaga ttgaccccag tttctttcaa ttctctgagc
    15361 agaatttcag agccttctgt cgcaaaataa gtctgaagac ttttggcaat cacgccacct
    15421 agactttcaa tactagccac ttcctctgaa tctgcctgat acagattttc aattgaatgg
    15481 aaatattgaa gtaaaagctg actagccttg cttccgacat gacgaattcc caaaccaaat
    15541 aagagcttct cggcagaatt ttcctttgat gcttggatag cctgatacag tttagcagcg
    15601 gacttttcct taaccccctc taaaaggagg aaatcctctt cttgcaaacg ataaatatcc
    15661 gccacatcct tgactaaatt agcagcaaaa agcttctcaa caatagatgg accaaggcct
    15721 gtaatattca tagcatcacg agaagcaaag tgaatcaagc cttccatgat ttgagcaggg
    15781 caacgcggat tgatacaacg tagggccact tcatcttcaa agtgcaacaa gtcagagtta
    15841 caacttggac agtttgtagg gatatctagt ttttcttcag aaacccgttt ggactctacc
    15901 acacgtaaaa cggcagggat gatgtcacca gccttatata caatgaccgt atcgtctttt
    15961 cggatatctt tttcagcaat ataatctaca ttgtgcaggg tcgcacggct aacagtcgta
    16021 ccggcaagtt gtactggtgt tagattagca gttggagtta caacaccggt acggccaact
    16081 gtccagtcaa ctgataagag ttgagcttct ttttcttcag cagggaactt gtaggctact
    16141 gcccactttg gagccttaac ggtaaaacca agttcttctt gacttgctag gtcgttgacc
    16201 ttgattacca ctccatcaat atcgtaaggt agattttccc gttcctgtcc tacttcttgg
    16261 ataaaattcc agatttcatc tatgttttca gccaagattc gcttaggatt gaccacaaaa
    16321 cctagttgtt ctaggtactt caaacccttt tcttggctat cacgagttga aggactggct
    16381 tcttgataga gaaacgttgc aagattacgc tttgcaacta ctgctgtatc caactgacgc
    16441 agagttcctg ctgccgcatt acgaggatta gcaaattcag gctctccatt ttcttggcgc
    16501 gcttggttaa cttggtcaaa ggaagcgcgt ggcatgtaac attccccacg aactgtgata
    16561 tctagttctt ctggcaaagt caaagggatg tccttaacac gcttgaggtt ttctgtgata
    16621 ttttcaccaa ttgaaccatc tccacgtgtt accccagcaa ccaaaatccc cttttcataa
    16681 gtcagcgaga tagataagcc atcgattttc agctcacaaa tataggtcgg atgagccact
    16741 tccttacgaa cacgcgcatc aaaagcatct agctcctcac gtgaaaaagc atcctgcaaa
    16801 ctataaagag gatactgatg actgtatttt tcaaaaccat ctaaaacctt gccaccaaca
    16861 cgatgagtcg gactgtctgc tagcacttgc tctggataag cagtttctaa ctcgaccaac
    16921 tcacggtaaa ggcggtcata ctcactgtct gaaaccgagg gattatcgct ggtatagtac
    16981 tcagtcgcat agcgattgag caaagcgact aactcattca ttcttttatt cataagacca
    17041 ttttaccata aaacaagccc tcctcacaaa cgagaagggc ggaaaaaaca cttagtttga
    17101 aattattttt gaaactcaag caaccttata tcaatttttc aaaatgagtt cgaacataaa
    17161 taaacgatat acaagacaag atgataacac cacttccaat tatcaggaaa gaagagagat
    17221 gtacacttgg caagactgtc ataaatcctt ttgcaatagg cataaataga atagctaagg
    17281 taaaaattgt actcagtact ctcccaagaa attcgctctc aaccttggtt tgtacttgag
    17341 taaaaaagtg aatattaaaa atcgtcataa acaattcaca aactaaattt ccagaaaagg
    17401 aaagaaaagt tggaagtggt aatcccatca taaaaactcc gacacctgtc aaagccagta
    17461 aaatcaaaag attataaata ttagctttaa ttttactagc tagaagagcc ccaatgatgg
    17521 aaccaatagc ccccatagtt aaaatacttg cataggctcc ttctgacccg taaagctgat
    17581 tcgaaaaggg aagtagaaat tcaaaagctg caaaaaagaa attaacgctg gaagctacca
    17641 gcaaaaggaa gaaaatttct tgctgatgcc agatatagtg taacccatcc ttgatatcta
    17701 caaaaatatc tctcccagta aaagcctttt tctcttgaac ttttgcttcc tcttttggaa
    17761 ggaaagccac tagaacaaaa gcaatgaaaa aagtcagcga gtctagcagt agcgtcatat
    17821 ggagacttgc aaactgtaaa acaaggaagg aaagaacagg agagctaaca cctacaacct
    17881 gcaaaaccag ctctaagcga gaattataga tcacaatctc atctttctcc accacttcag
    17941 ttatgatagc tttattggct gtgcgagaaa aggcaaaagc aatagtctgc acaatgttag
    18001 caacaatcaa agcgccaatc atccagctat cattccttat gaaagaaata gccagacaaa
    18061 gaatcccaca aacaagatct gccgtcatta aaatcttacg acgagaaaaa cggtctgaaa
    18121 taactccgcc aaagggattg acgagaatag atgtgacgag ctcagaaatc tgatacattc
    18181 ctaaaactgt ctgtcctata gtccccatag aagccaacca gacactattt ccataatcat
    18241 agagcatatt tcccatttta ttgatagccc cacggctaat caactgcact gcatagcgat
    18301 tcatattaaa gctcctctca aattttgaaa ctattgtatc aaaaccgaaa ggagcttttt
    18361 attttttccc ttatttggga aaattaactt ttgacaaatt tttcgtagtg ttcctgataa
    18421 taggctactt gctctggaag acctaacaca tcaaaaatat gcatggcctc ttgcatctgc
    18481 ttacagcctt ctttacactg tcctttttga tataaggcca aaccttttaa ataatggaaa
    18541 acattacgct cataaagctt aatacctttg tcaataatct tctctgtata agcctcaaaa
    18601 tagttggcat tataaaaaga agaatgctct aaacaatgct ggtaacaatt gagggccaaa
    18661 attaacacta atctcttatg gcgactaatc tcttggtaaa attcctccct ctccataact
    18721 tctctaccaa tccgagtgac atagtctaca tcgtagaaac tatagaggtt accgaaaaga
    18781 atcaactcat acatggtcca ttcttctgtt ttgaagagat aatctgctac cttatccaaa
    18841 tcatcctgct tcatatcata actcgcatct ctttgacaaa tcagaccttg tagcaaaatc
    18901 cagttcagct caaaataaag gggagtcgtc gaactcttag acttttcaag ttgttctctt
    18961 tgaagctttt gaaaacctgc aatatcgttt gaatagtaaa gtgggataat ctgtgccatc
    19021 atagacacat gttcatgatt atgaaaattc cttgccttat ccatgaaatt ttcgattgtt
    19081 acatgaatgt tatccaaaat ctcaaagaaa cgggagactg ccaggtcaga ctccccaagc
    19141 tcaaagcgag ataactgaga ggtagagcag gattcgcctg ctgcttcctt taaagaataa
    19201 tttccacttg ttcgaaattc acgaaatact tttccaagat gttccatctt tacacctgct
    19261 ctgataattc ttcccactca agcatagctt cttcctgacg atggctgatt ttgtccagct
    19321 cagcctgtaa ttccatgagt ttgtcggcat cgtttgtttc caacatttgt tcagaaatgg
    19381 cttggctttg actttctagc tcttcaattt cagcttctag actttcgatt tgtcgcatga
    19441 gtttgcgaac ttctttttga ctttctttct gggcctgata gtcattgact ggacttgctt
    19501 cctttgcttg attgctagtt gaagcttcct cagtctgact catttctgct gttactttct
    19561 tctcaacata gtagtcgtaa tctccaaggt agagagttga accattctca gacaattcca
    19621 aaacatgagt tgccacacga ttgataaagt aacgatcatg actgacaaaa agcaaggtac
    19681 catcaaagtc aatcaaggca ttttctagca cttccttact atcaatatcc aagtggttgg
    19741 tcggctcatc cagaatcaaa aagttattgt tttccataga caatttggct aaaagcaaac
    19801 gagctttttc gccaccagat agcatgccga ctgatttttt aacatcatct cctgagaaaa
    19861 ggaaggctcc aagacggttg cggatttcaa cttctggtgt cagtttgaaa tcattccaga
    19921 gttcatccag caccgtatta cttggtgtca gcttgctttg ggtttggtca tagtaaccaa
    19981 cctcaacatt agcgccaaag cgcttttctc ccttgataaa cggaatttga tccacgattg
    20041 atttgataaa ggttgatttt ccgataccat tgggaccaac gatagcgaca gcattcatct
    20101 tacgaagatc taggttgata ggttgtgaca agacttcccc gtcataacca atagcagtat
    20161 tttcaacagt caaaacaaca ttgcccgacg tttgttcaga ctggaaggtc atgttggctg
    20221 ctttcttgcc agcttcaggc ttgtccaaac gctccatttt ttctagttgt ttacggcgag
    20281 attgagcacg tttagtcgtt gaagcacgaa ctagattgcg attgacaaag tcttccagag
    20341 cagcgatttc cttctgttgc ttttcatagt tttttgcctc agtaactagc ttttgctcct
    20401 tcaattcgac aaaacgagag taattcccca catagcgatc caaggaatgc ttggtcaaat
    20461 ctagcgtaat tgtcgcaacc ttgtccaaga aataacggtc gtggctgacg ataatgaggg
    20521 caccgctata gtttaccaag taattctcta gccaggcgat ggtttcaata tccaagtggt
    20581 tagttggctc gtccaagacc aagagattgg gcttttcaag gagcattttg gcaagtgcca
    20641 aacgagtatt ttgaccacca gaaagctcag caattttcat ctgccacata gactcgtcaa
    20701 acttgaatcc attcaaaatc gttcgaatat cagcttcata ggtaaagcca cctgcttggc
    20761 gaaaattctc agataagcgg tcataatctg acatcagttt atccaaatcc tcaccagact
    20821 tttcacccat ctccagctcc atctgacgca gttgtctctc cgtccgacgc aaattattaa
    20881 agacatgaag catttcatcg tagatggtat tttcagactc aaaacggcta tcttgggcta
    20941 ggtaagacag agaaatatct tttttcttat tgatttctcc gctagttggc tcctcttctc
    21001 caactaaaat cttcaaaaga gtagacttac ctgcaccatt tttcccaaca agggcaatcc
    21061 gatctcgttc atcaacctgc aggttgatat tatcgaaaag aacctctcct gcaaaagaac
    21121 gttcaatttt attagcttgt aaaataatca taagagtagt atagcatgtt tccctaaggc
    21181 attcaagata atcgtaagtc ttttagtaca acttttataa cataaaataa actaaattat
    21241 gtatatttta tattagattc ttcactatct tgttggattt tctaaccagc taatcttgtt
    21301 tcaaatagtt atcgcacaag cctattattt aattcttttc attatttacg tacgtatagc
    21361 agattgaaat aagatgagaa caaatcgatt gggaaagtaa aattaatttc tataaatgtt
    21421 ttagcaattg tttcgtacta ttttagattc agtctactat atatacaata ttttcggaac
    21481 attcaacttt ttaactctat ttattactag atttcataat taaaaagcct actgaccaag
    21541 ctagaaagct tgatacaata ggctttttaa agactgatta tttaacagcg tctttaagag
    21601 ctttaccagc tttgaatgct ggtactttag aagctgcaat tgtcatttct ttaccagttt
    21661 gtgggttgcg acctttacgt tctgcgcgct cacgaacttc aaagttacca aaaccgatca
    21721 actgaacttt ttcaccagct gcaagatagt cagctactgc tgcaaataca gcttcaactg
    21781 ctgctgctga gtctttctta gtcaattctg tagcttctgc tactttagcg atcaaatctt
    21841 gtttgtttgc catgttaatg attcctccaa ataatttcta attaacaaat ataatcatat
    21901 cctaaaatcc cttttaggtc aagttaaaaa cgctatttct tcccatttta tttatttttt
    21961 taggagtggt aacgtatcaa aatagcccaa gcgttctcac ccgtgtgagt ttgaataatg
    22021 gaacccgttt ccaaaacaga aattggcttt tcaacataag cttgtaagct ttctttcatc
    22081 tcttttgccc aatcatcact accagaatat gaaattccaa tctctgctac agcacgttca
    22141 gaaagcgatg ttatcaactc atctaaccat tttttaaatg ttttagttcc acgaccttta
    22201 accattggct gcaattcatg gtctttcatt tgcatgacaa cacggatatt gagaagagag
    22261 ctcaacaagc cagttacacg gctaattcgt ccacctttga caagattttc caaagttgaa
    22321 acaccaatat aaagctctgt atggttttta acctcttcta catgacataa aattgcctcc
    22381 atatctttac cttcttgagc taacttcgca gcctcaacaa cttggaattt cagggcttgg
    22441 tcagtgaagg aactatcaac aacagtcaca tctgcagtag ataggctagc accttggcgt
    22501 gctgcttcta ccgtacccga aagagcatgg gacatatgaa tagcaagaat ctggccacca
    22561 tctttgcata ggtcttcaaa aatctcagca aagacaccta caggtggctg acttgttttc
    22621 ggaagattct tactttcttg catcaactga agaaatttac cttcttcttt caaatccgca
    22681 tcagaataaa caacattatc aatcattaca gataatggaa caattgtaat atctaattgc
    22741 tttactagtt caggttcaat agtaacagat gaatcggtta caatcttaat ttttgtcata
    22801 gtatcaatct ttctatttta ggattcagat tggtttcctt acttctaatt atatcaaaaa
    22861 aaagattaaa aatcctaatg gagtcaatca aattttccgt aaaatttgat ataatcaact
    22921 tataagaaaa gaggtgtcct atgattaaaa aaatttaccc catttttacc attttactag
    22981 gtgctgctat ttatgctttt ggactgactt attttgtagt tccccatcat ctctttgaag
    23041 gaggggcgac aggtattacc ctcatcacat tttatctttt taaaatccct gtttccctca
    23101 tgaacctgct gattaatatt ccccttttca tcctagcttg gaaaatcttt ggagccaaat
    23161 ccctctattc tagtttacta ggaaccttag ctttgtccgg ctggttagct ttttttgagc
    23221 atattcccct tcatattgat cttcaaggtg atttactaat cacagccctt atagcgggaa
    23281 tcctattggg aattggcctt ggaattattt ttaatgctgg aggtacaact ggtggaactg
    23341 atattctagc tcgtattctc aacaaataca ctcatatatc cataggaaaa ctgctcttta
    23401 tcttagattt ttgtattctc atgttgattc tcctaatctt caaggatttg agattggttt
    23461 cctacacgct tttgtttgat tttattgttt ctcgtgttat tgatttgatt ggtgaaggag
    23521 gatatgccgg caaaggcttt atgattatca caaaacgtcc tgaccaactt gctaaggcga
    23581 ttaatgatga cctcggaaga ggtgttactt ttatttctgg tcaaggctac tatagtaaag
    23641 aaaatttgaa aatcatctac tgtattgtcg gaagaaatga aattgtgaaa acgaaggaaa
    23701 tgattcatcg aatcgatcct caagccttta taactattac agaagcccat gaaatcctag
    23761 gagaaggctt cacctttgaa aaagaataaa aagaggtaat gtcgtgacct caaaagttag
    23821 actaaatcat ctatcttttg ggttacagac aacctctttt ttgttttatt tactcaagct
    23881 cttaagacca attccgagtt acttcttcat cagcctttaa ctgatccact aattggtcaa
    23941 ctgagtcaaa tttggtcata tcacgaatgc gatcaagcca ataaaccatg acggtttccc
    24001 cataaatatc ttgattaaaa tcaaaaatat tgacttcaaa acgtgcttct tctccatcaa
    24061 aggtcacatt tttcccgaca ctagccatag cacgatactt ctgtctttga atctcaacat
    24121 caacgacata aacgccatct gctggcatat aagtacggtc taaaagcact aaattcgctg
    24181 tcggataacc aattatacga ccacgagcat taccatgaac caccatacct cttgatggaa
    24241 gcggtgcccc caaaagtttt cctgcttctt tcacatttcc atctaaaata gcttgacgga
    24301 tacgagttga actaatcttt cctttctcat cttctacagg tggaacaatg ataacttctc
    24361 catcaaagta atcctttaaa tcttctgctg tttttttgtc agaaccaaat gtataatcaa
    24421 aacctgcaac aataattttg gcattcatag ccttgatata agttgcaaag aattcttgtg
    24481 cagtgagact agcgaattga ctactaaaat caaggagata taattcttct acaccttcgc
    24541 gctttaattt tctttcacgt tcagcagggt tcaaaatatg caaaaacaaa tccggatgat
    24601 aaggctctaa agcgatcttt ggagattcat taaaggtcat aacgacgata ggtaataaat
    24661 cctttctcgc agccttgttg gcaacacgaa ataattcttg atgcccctta tgtatgccat
    24721 caaaatagcc gagaacaacg actgaatcag atggtgtgcc aatatctttt tggtttttta
    24781 taggaatagt aataatcata aaataattat atcatagcga tagctatttc tggaacagaa
    24841 aatctgaaat gttgtttttt tcacatgaag tgtacctgtt ttcaaaaagc actttattct
    24901 atcgttgctt aactatgaac tttgcaatat tcttctcaaa aacttgtagg acatcttcaa
    24961 aattttgcaa ggagtgatta gacttgttcg gtaaccataa agtgtcatac tatgttatgt
    25021 atgaaaaagc aatgcaacta actcctgaga actttaaatt actaattggt gccgaaaagg
    25081 tagaatttag aatcgaggta cacctatggc tgtaaaattt acaaaatgag acaacttggg
    25141 caagatgttt gaagaatttc ctaaactccc tgatttgaag caagtcactt tccctgatga
    25201 caaagaaaaa agccaaaaca gcaaagaaaa actagatgac tgctttccaa caactcccat
    25261 ctagtgtgct tcagactggg ctatttttct ctccatctgt tagcttggat tctcagaccg
    25321 tttcagctaa agaatatctt ttcccttatc agaaggaacg gctcaaacca ttcagacaag
    25381 tgaagggacg acaagccaat atttgaaacc agatagcagt tcttatagtc aattgaaata
    25441 aaatctgaag aaatcgagta ggaaactcat atcaattttt aacagtgttc tattccagat
    25501 tcatactcaa tgaaaattaa agtgcaaact aggaagttag ccgcaggctg tacttgagta
    25561 cggcaaggtg aaactgacgt ggtttgaatt tgattttcga agagtatcaa tccactatac
    25621 atattaaaaa gtatggatat caaaacaaaa aaacaggctc tccgaaaact cggaaagcct
    25681 tattttaagc tgtttctagc ttcctcacct ttacatttca aggttcggga taaaaggttc
    25741 aatggacctt tttatttagc gattgggtaa acagaaactt gttttttatc gcgtccttta
    25801 cgttcaaagc gtactacgcc ttcaacttta gcgaacaaag tatcgtctcc accacgacca
    25861 acgtttacac ctggatagat gtgtgtacca cgttgacggt aaaggattga tccacctgtt
    25921 acagtttgtc cgtcagctgc tttagctcca agacgttttg cttgtgaatc acgtccgttt
    25981 gatgtagaac ctccaccttt tttgtgggcg aaaagttgca agttgttaag agtcattttt
    26041 aacataatgt tttcctccgt gttagttttc tgtgataact ctggtttgga cgaactcaga
    26101 atagttctcc gataagtttg ccatacctaa gaaaaatgat tcaaagaata actgggtcat
    26161 ttctctctgg tgtgaaggaa gatcttttgg tatttcaacc atcagatagc caccttcatc
    26221 ttcgtttaat tctaggattg gttcatagcc tgcaaatttc tcaatagaat tgataaagtt
    26281 aatggcaagc gtagaaaccg atgcacacac gacatctaag ccgtattcgc cactctcggc
    26341 gtgtccagta atttccgcac ccctcagctc gccatcttcg gctctctcaa agactgcttg
    26401 tatcatgtgt tctccttaaa attaagcgtt aattgcgttg atgacaactt ttgtatatgg
    26461 ttgacggtga ccttgtttac ggtggctacc ttttttaggt ttgtacttgt aagtaacaac
    26521 tttcttttgt tttccttgtt tttcaacagt tccaactaca gtagctccag caacaagtgg
    26581 agttccgaca acagtgtttt caccaccaac aagaacaact tcgttaaaag taacttcttg
    26641 accagcttca acgttcaatt tttcaatgta aactgcttga ccaacttcaa ctttaacttg
    26701 ttttccgcca gttttgataa ttgcgtatgt gctcattatg cacctcctat gatttttatg
    26761 ggtttccccg attttttgtg aagactcgcc tagcatcgtg ggacaaacca cttaaacttg
    26821 aataatcaag caacgatgtt cgtgcggttg cacaggatcg tgcatagtca actcttcaag
    26881 tatagcatat ctcctatttt cttacaagta ataacaccta aaatcaagct ttttctttta
    26941 cttttttctg ccactaggca aaaagcatac tgagataaaa aatgctcatc ataataggaa
    27001 caccaagaat ggtcttttca tgatagaaaa tcgtcaaata ggctgaaaag acaacgccaa
    27061 ggacaaaact actaaacagg ctaacaaata tgaacccttc acgcaaaaaa ggagtgtgct
    27121 tagttcggaa ataatctcca aaagctagca ttgttcgttt gatattccct gtcataaagg
    27181 cattattgta ggcaataccc gaaacctcac caaaagcagt tgtcaccagt cccatacaga
    27241 aggccaaggg ctgcactaga tagatattat ctacagtttg tggcacaaaa ccaataatta
    27301 ttgataggat tgctaaagga atcaaggaca gaataggttt tttcacaatt ctcaattttt
    27361 ccttataaat cgttaataaa aagactccca ttataaacgt tattaaggtt aaaagcttat
    27421 ccctaacatc cgaaacatta ttttgtatta gctctaccga aagaaagaca acatttccag
    27481 cttgtccagc tacaagggta ttccctcgaa caataaaagt gtaagcatcc acatatccag
    27541 cacaaaatgt caaaaaagtg ctaacctttt agactgacgt gatatttttc ttataggtaa
    27601 taacctcatt ttccctccca ttgtattttc tcttagaaat attgtaccat ttctttctaa
    27661 aaaatcgtag actaccattt agattttact attagcataa aaataataat agacaactat
    27721 ttatccaaaa atagatagag gtaacatgtt tgcaaacaaa gcatacgaac ctttagtaaa
    27781 atcatttcca tgaaactaga atagagcccc tcttagcaaa aatcattatt ttaatttatt
    27841 tctaatcact ccttgacata aataactctc accaataaaa gactatgtct taaaaaaatg
    27901 gtataataaa atcaatacct gggcttgatg gctatgctac taataacaat taggagagaa
    27961 aatcaggcac ttgttaacaa caaggattat ccccttgaga tgaaaggaac tttagaaatc
    28021 ttatgatgaa catgcaaaac atgatgcgtc aagcacaaaa acttcaaaaa caaatggaac
    28081 aaagccaagc tgaacttgct gctatgcaat ttgttggcaa atctgctcaa gatcttgtcc
    28141 aagcgacctt aactggcgat aagaaagttg tcagcattga tttcaatcca gctgtcgttg
    28201 acccagagga ccttgagact ctttctgata tgaccgttca agccatcaac tctgctcttg
    28261 aacaaatcga tgaaactact aagaaaaaac tgggtgcttt cgctgggaaa ttgccattct
    28321 aaaaacaagg agctagaaca atacttgtcg ataacaaagg ctaagaaagg tgcaaaaatt
    28381 ataaaaaacg catagtatca agcaacctaa aactttgggc tctataatat ttgtagtggg
    28441 taaatccact atagatatta tggagcctat tttattgtag aaaaaaatcc cataagatct
    28501 ataatgaaaa gcgaccaaac aactcattag aaagattcat atgg
//
LOCUS       DAVBDG010000024        28248 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBDG010000024 DAVBDG010000000
VERSION     DAVBDG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.024"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..556)
                     /locus_tag="VQ526_001011"
     CDS             complement(<1..556)
                     /locus_tag="VQ526_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2554745.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNH"
     gene            1180..1362
                     /locus_tag="VQ526_001012"
     CDS             1180..1362
                     /locus_tag="VQ526_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2554746.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            1372..1722
                     /locus_tag="VQ526_001013"
     CDS             1372..1722
                     /locus_tag="VQ526_001013"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2554747.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYGDSAWEHLRAVTGID
                     LLSILTDIANKKQG"
     gene            1730..2920
                     /locus_tag="VQ526_001014"
     CDS             1730..2920
                     /locus_tag="VQ526_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2554748.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKYFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(2996..4558)
                     /gene="guaA"
                     /locus_tag="VQ526_001015"
     CDS             complement(2996..4558)
                     /gene="guaA"
                     /locus_tag="VQ526_001015"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2554749.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4700..5398
                     /locus_tag="VQ526_001016"
     CDS             4700..5398
                     /locus_tag="VQ526_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2554750.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(5441..6337)
                     /locus_tag="VQ526_001017"
     CDS             complement(5441..6337)
                     /locus_tag="VQ526_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2554751.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGTLMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLIIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(6346..7281)
                     /locus_tag="VQ526_001018"
     CDS             complement(6346..7281)
                     /locus_tag="VQ526_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2554752.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(7278..8003)
                     /locus_tag="VQ526_001019"
     CDS             complement(7278..8003)
                     /locus_tag="VQ526_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2554753.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQIDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(8087..8722)
                     /locus_tag="VQ526_001020"
     CDS             complement(8087..8722)
                     /locus_tag="VQ526_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2554754.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8724..9551)
                     /locus_tag="VQ526_001021"
     CDS             complement(8724..9551)
                     /locus_tag="VQ526_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2554755.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9573..9908)
                     /locus_tag="VQ526_001022"
     CDS             complement(9573..9908)
                     /locus_tag="VQ526_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HET2554756.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(9909..10625)
                     /locus_tag="VQ526_001023"
     CDS             complement(9909..10625)
                     /locus_tag="VQ526_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2554757.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHIFDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYDFNGIEQYISQEDYNRYLSD
                     LNEDKMKVEIERAEKEADITIIMLQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIIY"
     gene            11148..11759
                     /locus_tag="VQ526_001024"
     CDS             11148..11759
                     /locus_tag="VQ526_001024"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2554758.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11804..12532
                     /gene="rlmB"
                     /locus_tag="VQ526_001025"
     CDS             11804..12532
                     /gene="rlmB"
                     /locus_tag="VQ526_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2554759.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(12571..13482)
                     /gene="trxB"
                     /locus_tag="VQ526_001026"
     CDS             complement(12571..13482)
                     /gene="trxB"
                     /locus_tag="VQ526_001026"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET2554760.1"
                     /translation="MYDTIIIGAGPAGMTATLYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(13548..13772)
                     /locus_tag="VQ526_001027"
     CDS             complement(13548..13772)
                     /locus_tag="VQ526_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2554761.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13850..14593)
                     /locus_tag="VQ526_001028"
     CDS             complement(13850..14593)
                     /locus_tag="VQ526_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2554762.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14593..15393)
                     /locus_tag="VQ526_001029"
     CDS             complement(14593..15393)
                     /locus_tag="VQ526_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2554763.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(15551..15907)
                     /locus_tag="VQ526_001030"
     CDS             complement(15551..15907)
                     /locus_tag="VQ526_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2554764.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15920..16432)
                     /locus_tag="VQ526_001031"
     CDS             complement(15920..16432)
                     /locus_tag="VQ526_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2554765.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(16432..16926)
                     /locus_tag="VQ526_001032"
     CDS             complement(16432..16926)
                     /locus_tag="VQ526_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2554766.1"
                     /translation="MEIRLAFPNEVDAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            17061..17507
                     /locus_tag="VQ526_001033"
     CDS             17061..17507
                     /locus_tag="VQ526_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2554767.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            17504..18151
                     /locus_tag="VQ526_001034"
     CDS             17504..18151
                     /locus_tag="VQ526_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2554768.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(18172..18753)
                     /gene="pdxT"
                     /locus_tag="VQ526_001035"
     CDS             complement(18172..18753)
                     /gene="pdxT"
                     /locus_tag="VQ526_001035"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2554769.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18754..19629)
                     /gene="pdxS"
                     /locus_tag="VQ526_001036"
     CDS             complement(18754..19629)
                     /gene="pdxS"
                     /locus_tag="VQ526_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2554770.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19781..21160)
                     /locus_tag="VQ526_001037"
     CDS             complement(19781..21160)
                     /locus_tag="VQ526_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2554771.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            21454..22377
                     /locus_tag="VQ526_001038"
     CDS             21454..22377
                     /locus_tag="VQ526_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2554772.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            22437..23042
                     /locus_tag="VQ526_001039"
     CDS             22437..23042
                     /locus_tag="VQ526_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2554773.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEEFDWENL"
     gene            23060..24304
                     /locus_tag="VQ526_001040"
     CDS             23060..24304
                     /locus_tag="VQ526_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2554774.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGNADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(24527..24784)
                     /locus_tag="VQ526_001041"
     CDS             complement(24527..24784)
                     /locus_tag="VQ526_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2554775.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKMEQAKLREEYIEGYRRAV
                     RHNIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24826..26862)
                     /locus_tag="VQ526_001042"
     CDS             complement(24826..26862)
                     /locus_tag="VQ526_001042"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2554776.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEYFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(27122..28039)
                     /gene="glyQ"
                     /locus_tag="VQ526_001043"
     CDS             complement(27122..28039)
                     /gene="glyQ"
                     /locus_tag="VQ526_001043"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2554777.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEILLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
BASE COUNT         8608 a         6158 c         5102 g         8380 t
ORIGIN      
        1 tgtggtttga aagagtacaa cgtccagagt atgaaataca catagctcca tggacaaagg
       61 cttcaatttc aacatctgta cgtttgcgga tttcagctaa ttcttccatt gaaacctcac
      121 gcgctaaaac gacacgagtc aagcctagct ctttccagaa ctcaagggtt tcatagttag
      181 tggcactggc ttgggtagaa aggtggattt caaggcctgg tgcttcagtc gctgcaatca
      241 taatcaaggc tgggtcagat acgataactg ctgcaatccc gatatcacgc agtttacgga
      301 accactcacc agcaccagct tcatttcctt cgtgcataac catattagcc gctacataga
      361 ccttggcacc atacttggcc gcaaactgca cgccttcttc catctgttcg aaagtaaagt
      421 ttcccgcacg gctacgaaga ccataggcct gaccaccgat aaagacagca tctgctccat
      481 actgaacagc tacctttagc ttctctaaag tccctgcagg tgataaaacc tcaggacgtt
      541 ttaatgtttt tgtcataatt tctcctattt gcaatataaa agtttgacat ttttccttct
      601 cattttatag taaataaagg ataaaatcaa gcctagacgg attgttttga caattctaat
      661 cttttagact ttgcataata actattaaac agtcgaaaat taccccagtt ttaacaagta
      721 gtcaaaaagt ctaaaaaagt aaaaaccagt atcccaagat actgactcgt aaaaatatat
      781 aaattagtcc ttagatgact actcccactc tgttgctcct ataatggaaa aggattgata
      841 cagtctagaa tatcgctttt ttatttatta ttatgatata aaactgttag aattgatttt
      901 ttgttttata cccaaatatg aatagagacg tagctaataa gttgaataac atatttggaa
      961 aaatgagaaa tagcaatatt actagaactt ttagaaacaa tcaatattta aaaagaaaaa
     1021 agctcaaaga atttattaaa ccatgttata atagatatag ctataatttt atttataaca
     1081 agaggataga aatgaccgaa ttagaaagaa aaaatcgaaa aattagctaa gaaatattct
     1141 gataacttaa acatcaaagt tcaagagaga gttcgtgaaa tggcaaatga taataagagc
     1201 cattatttga tatacagagt tttaggtatt tcatttgaag aaggagaaaa tatcgatttg
     1261 tatcaaaata aaggtcgttt tttatacaaa tatgctggtt catttttaga agaagctgca
     1321 gtactatcct ttaacgaaaa atttggtaca gaaaatactt aaaaagttaa cattcctaat
     1381 tctgaaagta caaaacctaa gacttttgaa attgattgtt tagtcggaga aaaacacgca
     1441 tacgaaataa aatggtggga tgcaactaca gatggagacc atataactaa agaacacact
     1501 agaataaaag ttattcataa caaaggatat ataccaattc ggttaatgtt ctactatcca
     1561 aatagaactc aagctataaa aattcagcaa actttagaaa cattgtataa cggtattgga
     1621 gggaaatatt atggagattc tgcctgggaa catttaagag cagtgaccgg tattgattta
     1681 cttagtattc taacagatat tgcaaataaa aaacaggggt aaaatcaaaa tgacagtatt
     1741 aaaaggagat aacttagaaa tattaaaaac tattgaatcc tcaagtattg atttaatcta
     1801 tatggaccct cctttcttta cacagaaaac ccaaaaatta tctaataaca aaaatattat
     1861 gtattcattc gaagatacgt ggacttcgat tgaggattac aaagaatttt tgtctgtaag
     1921 attagaagaa tgcaaaagag tgctaaaaaa tagtggcagt attttcgttc attgtgataa
     1981 aattgcaaat catcatatta gattaatttt agataatatc tttggagcag atatgtttca
     2041 aagcgaaatt atatggaact ataaacggtg gtctaattca aaaaagggat tattgaacaa
     2101 tcatcaaaac atttactttt attcaaagtc aaaatatttt aaatttaata caatttttac
     2161 agagtattct tctactacaa atatcgacca aatactagtg gaacgaaaac gagatggaaa
     2221 ctctaaaact atatataagg ttgataataa tggtaactat attctagcaa aagagaaaaa
     2281 tggagttccc ctttcagatg tttggaatat accatttctt aatccaaaag ctaaagaaag
     2341 agtaggttat cctacacaaa aacctattct gttattagaa caaattataa agattgctac
     2401 tgataaaaat gatatagttt tagacccgtt ctgtggaagt ggaactactt tagtagcctc
     2461 caagattttg aatagaaatt atatggggat tgatttatct gaggaagcta tcaatataac
     2521 tcagcaacgt ctggaaaatg ttataaaaac aagttcaaat ttattgaata aaggaatcga
     2581 agcatataga accaaaactg aggaagagga aaacattctt aaattattac aggcaaaaat
     2641 tgttcaaaga aataaaggaa ttgatggttt tttacctaaa cattttcaaa aaaaaccgat
     2701 acctataaaa attcaaaaaa ataatgaatg tctgaatgag agtatctctt tattacagaa
     2761 tgctataaac tccaaaaaac ttgattttgg agtagttata aaaactcatt cagataattt
     2821 attatttgat tttgatacga ttcctgaaaa tataatagta gttgaccatt ttgaacttac
     2881 aatcgaaaaa tggttaagta aaagtcaaca gttactatag tttgcaaatt atttacagaa
     2941 agaacaaaaa atgctctgaa acactgattt caaggcattt tttgttagtt taaaattact
     3001 cccactcaac tgttgcaggt ggtttacttg taatatcgta gacgatacgg ttaacatgat
     3061 ccacttcatt tacgatacgt actgagattt tttgaagtac ttcccatgga attttggcaa
     3121 aatcagcagt cataccatcg atagaagtga tagcacggat tgcaatcgtg tagtcatacg
     3181 tacgaccgtc acccataaca ccgactgaac gaacgcctgt gttaacagtg aagtattgcc
     3241 aaatatcgcg gtcaagtcca gctttagcga tttcttcacg aagaatagcg tctgattcac
     3301 gaacggtttc aagtttctct tcagtgattt cacccatgac acggatagca agtcctggtc
     3361 ctgggaatgg ttggcgccat acgatatggt ctggcatacc aagctctgta ccaagagcac
     3421 gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa ttcaaactgc atatcttctg
     3481 gaagaccacc cacgttgtgg tgtgacttga tagtttgagc tgtatccgta ccagactcga
     3541 taacatctgt atataaagta ccttgagcaa ggaatttcac atctttgagc ttgcttgctt
     3601 cgtcatcgaa tacatagaca aactcgttac cgatgatttt acgtttttgt tcaggatcag
     3661 aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc tgctttgacg atattcaaac
     3721 caaacttacc accgagcatg tccataactt gatcagcttc gcctttacga agaagaccgt
     3781 ggtctacgaa gatacagatc aattgatcgc caatcgcttt ttggagaaga accccaacga
     3841 cagatgagtc aacaccacct gatagaccaa gaaggacacg tttatcaccg acggtttcac
     3901 gaattttttt gatctgcatg tcaatgaaat tatccattga ccagtcacct ttagccttac
     3961 aaatgttaag ggcaaagtta cgaaggatat catttccgta tacagaatga cgaacttctg
     4021 ggtggaattg gataccgtaa atgtgtttat ctgggttttc gatggctgcg tatgggcagt
     4081 cagctgatgt acctgtacga acaaagtcag caggaatctc agtaaccgca tcaccatggc
     4141 tcatcaaaac agtctgttca tcaggtgttg attcaaaaag cgctgatggt gtgtgagtta
     4201 gggttgattg accgtattca cgatttccag catcacctgc aggaacaact tttcctccaa
     4261 gtttatgggt caataactgc ataccataac agattcccaa aattggaatt cctagttcga
     4321 agatttctgg gtcaatatca aatgaaccat cttcatatac agaatttgga ccacctgata
     4381 gaataattcc tacaggattg acttcacgaa cttcagcagc tgaaatttta tggcttttta
     4441 gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat cagctggttg tactggctac
     4501 catagtccaa tacgatgatt ttttctacat cttgcaaatc agttgaaatg ttgctcatct
     4561 ttttcctttc tcaaaagaaa tatcttttct caatattcta tcacaaataa gggcgaatta
     4621 ccaactaaac aaggcgaagt ttgacttttt ctagtttatt ggggtacaat agagaaaaga
     4681 tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa atccatgatc agataaaaaa
     4741 ggatattgac gagcaccgtt gggctattgg tgagaggctt cccagtgaaa gagatttagc
     4801 tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc gtatctctat tagtcgaaga
     4861 aggcgtctta gagcgccgtg taggaagcgg cacctttgtt tccagtactc gagtacaaga
     4921 aaagatgcga gggacaacca gttttactga aattgtcaaa tcccaaggta aagttccctc
     4981 tagccagctc atttcctaca aaaaaaccat tcccaatgag caagaagttg ccaagctagg
     5041 aatttttcca acggacaata ttatccgaat ggagcgggtc cgctatgccg accaggttcc
     5101 cctagtttat gaagttgctt ctattcctga aaaattcatt aaggacttta aaaaagaaga
     5161 aatcacaagt catttcttcc aaaccttgca aaaacatggc tatcgtatcg gcaaatctca
     5221 acagaccatc tatgctcgcc ttgctaaaga aaagattgcc cactatttgg aagttgaaaa
     5281 aggacatgct attcttggat tgacccaggt ttcctaccta gaagatggta ctgcttttga
     5341 atacgtaaaa agtcaatatg taggcgaacg ctttgaattt tatcttgaaa ataattagat
     5401 taccacgcaa aaactctctg tcacggacaa agagtctttt ttatttttct aagaataaat
     5461 ctttcaaaga aatcaaagtt acagcaaaca atatcattgc cataccaaga aaatcaatgg
     5521 gataaaattg ttcattcatt attaagaagg caaagaaaat cgccgatatt ggctcaattg
     5581 aagccaacaa gcttgacttg accggtccta tcagactggc tcctttaagg aaagctgtat
     5641 aggcaaagac agtcccgata aggataatgc ctgcaaacgc aaggagaaaa tcaagactag
     5701 tcgggatatc ggcctgtaga acccctgtaa aaggaagggc gaccaaacct gctatgacca
     5761 ttcccacacc aatgatcaag ctgctccccc actttttaat caaggctatg ggtaaaatga
     5821 tatacagagc ataagtcaag gcagaaaaga gaccccagaa caggccagca ggtgtcatgg
     5881 ataactggtc caactgccca tgtgttgcga tcaggaaggt tcctccgatg gcgaatatga
     5941 tggaaactat ctctcccagt gtcggtgcca ccctatcctt gatacagcta taaattaaaa
     6001 ttccgacagg acaaacatac tgaagcaccg tcgctgttcc cgcattggtc tcctgaatag
     6061 cagacagata ggcgaattgg ttgagaaaaa gaccaatcag agcaaaaata agaagagaca
     6121 gcaaactctt tctatccttt aaaaagacca gtattttatc ctttgcagta gcataagcca
     6181 agagcatgag agttccacca gcgattaaaa gacgcaagtt ggtcaagacc agagccgaaa
     6241 ttccgtgtgc catcaggtat tggccactcg ttcctgacaa cccccaagca ataccagcca
     6301 caactgttag taaagtccct tttaaactat ttgacatgtc tctccttact cttctttgcg
     6361 aatcaagttc atcacttgat tgcgatctaa tatccattga gctctctcgt ccttttcata
     6421 ggtacgattc gataggaaaa tagtggcttc ttgcttctga cgattccaca tgataaaggt
     6481 acctgtatag cccgtatggt ctagccaatc tccttccaaa ttccatgcta aagaacgttc
     6541 cttgtcatcc aaaggagaaa aattttgatt taagtctctt gcaaaatcat ctgctaaata
     6601 gtgttctaaa aagatttgta aatcctttat agtcgaaaat aaaccagcac tcccagcatg
     6661 tctacccagg agacgagcct tgggatcatg cactatgcct gcctctacac ctctaactgt
     6721 tggaacagca agctcaactg gcccaaactt agtttccgtc attccccaag gtttccagac
     6781 ttgatccttt aaaatcacat ccaaatcttg attaaaaatt ctttccaaaa taaagcccaa
     6841 cagcaaaaaa tggacatccg aataaagaaa ggctggctga cttcgtctgt tgagatgaaa
     6901 catcgcttcc tttaattcag gggctgttaa aagatcacga ttaggaataa aaggatcaag
     6961 gtctgttgca tgagtcaaga gctggcgaat agtgatgtct ggataatcac tctcaggtaa
     7021 aaaatctatt accagtctat caatatctaa ttgacctatt tcccacaaga aggtacaaac
     7081 tgtgccaacc ccaacaacct tgctgacact agctaggtca taaactagtc ctgcctcagt
     7141 ctgcaagcca tgctctgggt cactctggcc taaatagaac tctgtccatt gattgtcctt
     7201 aaaatacgca aaagaggctc cgggataaat tcctgcctcg atttgttctt ctattttttt
     7261 aataatcttg gtccacttca tacttcttca aaccacaatt caacattatt tctatcttta
     7321 ccaaggaaga atttttcaga cttaggaata aaatattcag tagactcaaa cttctggcga
     7381 agacttgcta tgtctaattc attgaccaag aacttgagca tagataagtc ccaggtaacc
     7441 gtattgtcca cagtcaaatc ctgcccctga gctggaataa aatcaaggga tgccccaatt
     7501 tcagatgatt ccaagaaact ttcgatatca gttgggagat gtaattccat agaaatctca
     7561 aatttactta aagaaattga tgccaaatct atttgaaatt caggcttttc tcctacttct
     7621 actagacttg ctctgtcatc ttccgcatga atcaaaatca aatcatcttc tggtgagaaa
     7681 atttcaaaag cgtagccatt ttgaccttta tataatcgat gaatcgaatc tgttttagat
     7741 aagattcctt caatttctaa gggattttcc accttgacaa tcaatctagc tagttttttt
     7801 cttccctcta ccttacgagt acgcatactg ggagcttctt ctaaaaccag cttttcaaga
     7861 cccgtttggt cacctagtga cagaaaggcc gattcttcta acaaggcctt cattccaagg
     7921 gtttcaatat aaaatgtttc atttaatttt ctattattag cttttaaagt aggaataatc
     7981 cgtacaatct gatttacatt cataaattcc tccgtcactt tttattttaa aggattttag
     8041 aattttttac aagatattat gctcaaattt aacattattt agctatttag taaagatagt
     8101 ctttcaagga ttttgacaaa gcctgataac cagcaatact gaggtgcagt ccatcagttg
     8161 tatattcttt tttgagttgg cctgcttggt ctgtcaaaca atcaaatact ggcacaaatt
     8221 ccacctgcat ataggcagat gcaagctctt gataggcttg attccagttc tgaatttttt
     8281 cattcgagcg gatatagact gcctgctggt actcctctcc ctcattgaca ggcaaaatgg
     8341 aaagcaattt aatctctgtc aatggataat cgcgagcaac ggattgaatg atagcttcga
     8401 gattattgag agcctcattc acaggaacat cctttccgat atcatttgtc ccaatcagaa
     8461 gaaaaatttt atctactgct ccaccatata gatgagcatc aaggttctct agtaacagtc
     8521 ctgtctgata gccacgaatt cctcgattga caatcgtctt tgaagtccca aatagctcct
     8581 gtagaggata atactcgaca atggaatccc caataaaaag aatgtttggt tctacaacag
     8641 aaatgtgatt taggtgacga tatttggttt gaattttttc ttgttccttt aggagccaat
     8701 tttctaataa ctgtactgcc acttcattct cctttttcta accagttttc caagacttga
     8761 taaactcctc cttggctgtt ggctggtgct agagcagtcg ccacagcttt ggctttctca
     8821 tcagcatttt ccatcgcata ggcaattcca gccatttcaa gcatttcaac atcattttca
     8881 ctatcaccaa aagccatgat ttcttgggat ttcaagtccc agcgcttgag taattcctcc
     8941 aagccccatg ctttatgaat cccagcttgg aggatatcaa tgcaaccata gccactggat
     9001 acagctcgga cacggccatc aaagagagca ttgatttctt ccaaaaccga actcaaacgt
     9061 tcctcaccaa caaccatgct catcttgagc acaccaccaa agaggtcaga tgttaattca
     9121 tccacaaatt gcatccgttg gtagaatttt tcaatcattt ctggagtcat aaaactttca
     9181 agatctgtaa aaatcgtacc ttccttgaca aaatcaccct tcatccccgt tacaacaaac
     9241 tggtcctgac acgctcgacc cttgaaatga gccaaagcct tgttgacaat ggcgtcatcc
     9301 catgtctgag cttgaatcaa ttcattgttt tcaaaaatac gagcgccatt agcaacaacc
     9361 agaaccactc gatccaccaa gggactcagt agttgtctca tgcggtgaat ttcattgccc
     9421 gtcgcaatga caaaacgaat gcccctttga tccaactgat ctaaaatctt ttccaatcgt
     9481 gggagatcaa gctgacctct agcatccagc aaggtcccat ccatatctgt cgcaattacc
     9541 ttaatcatca tttactttcc tctggattca agctaatacc acttcaatcc cacatgttca
     9601 ttcatttctt tataggctgt atcaattcgt tccttagtcg cttcatctaa ctggtcacga
     9661 tgactgccat cctctataaa atcttccaaa atataagttt ggtaatgata caagggatat
     9721 ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg agctttagct
     9781 gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag aacaccacgt
     9841 tcagtccact tagcattctc ttcatctccc atagattcaa tccgttgatt ggaaatgaag
     9901 ttccccattt aatagataat gagtttctta tctccatctt tttcaaccgt ttcagatggt
     9961 tcaacaacgt gaggatgccc tccaaagata atatccgctc cccaatcgat catcttgtga
    10021 taaagagctt tttgttcttc agttggttcc aatcgatact caacacccat ctgaagcatg
    10081 ataatggtga tatctgcttc cttctctgcc cgttcaattt caaccttcat cttatcttcg
    10141 tttaaatctg aaagataacg attatagtct tcctgagaaa tatactgctc aattccattg
    10201 aaatcatagg aataggctaa caatgcaacc ttgatagcat tcacttcctt aatgaccagc
    10261 ggagcctgat cacgtggttc gtgcgtataa actccgattg gagtgattcc agctttctca
    10321 ataatatcgg ccattgaaat aactccctca atttgcgaat caaaaatatg attatgagct
    10381 aaatccagca catgataacc tgcctcctta atagcatcca taacttcaac aggagcatta
    10441 aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa atcaccaata
    10501 gctaaatctg cttgcttgag ccaaggagtc acatactcaa aattttcatg aaagtcatag
    10561 gtaccgtctt cttttttagc tgaaaagaaa agtccatcgt ggtatagcaa atccccgtgg
    10621 gccataattc tggcagtttt ttcctcttcc tactcctgag acttttgctt agtcccctct
    10681 tgagaaatag tatctcgttt ttgactagtc aagggattcc ttggaagctt tcaaacaaca
    10741 agaccaagcc cattgataaa ccaactgcta gcaagagtat cgccacaaat cccttattgc
    10801 tccacttgcg ataactccta aaaagtttac caagcccttc ataaaacgaa aagctaaacc
    10861 accctgattt cgattttgtc ttctttgtat cttcattctc cctactttct tatgcaagcc
    10921 ttttcttttt attatatcac agataagtat ttctttcaca attgaattga acttcctatc
    10981 tattttctat aaatcctaaa tgccataatg ctttcaatcc ttgtcatttt gtgatatcat
    11041 gtagaggtaa tgaaaggaaa tgaactaatc cacagtggct tattccaagt ataccacttg
    11101 ggctttggca gtagctaact gcactaaaca taatataagg agagaaaatg tctgcaatag
    11161 aacgtattac aaaagctgct cacttaattg atatgaacga tattatccgt gaagggaatc
    11221 ctactctacg cgcgattgct gaggaagtca ctttccccct atctgaccag gaaatcatcc
    11281 taggcgaaaa gatgatgcaa ttccttaaac attcccaaga tcctgtcatg gctgaaaaaa
    11341 tgggactccg cggtggtgtt ggactggctg ctccccagtt agatatctca aaacgcatta
    11401 tcgctgtttt ggtacctaat attgttgaag aaggcgaaac tccacaggaa gcctacgatt
    11461 tggaagccat tatgtacaat ccaaaaatcg tctctcactc tgttcaagat gctgctcttg
    11521 gcgaaggaga aggttgcctg tctgttgacc gtaacgtgcc tggctatgtt gttcgccatg
    11581 cccgcgttac tgttgactac tttgacaaag atggagaaaa acaccgtatc aaactcaaag
    11641 gctacaactc cattgttgtt cagcatgaaa ttgaccacat taacggtatc atgttttacg
    11701 atcgcatcaa tgaaaaagac ccatttgcag ttaaagatgg tttactgatt cttgaataaa
    11761 gaaaatcccg ttgcaagacg gggttttgtg ttataataga ggcatgaaaa caaatgatat
    11821 tgtctatggt gtccacgccg ttaccgaagc cctccttgca aatacaggaa acaaactcta
    11881 cctccaagaa gatctccgag gtaagaatgt tgagaaagtc aaggaactag ctacagaaaa
    11941 gaaggtgtcc atttcttgga catcaaaaaa atctctctct gagatgactg aaggtgctgt
    12001 tcatcaaggt tttgttctac gagtgtctga atttgcctat agcgagctag attacatcct
    12061 tgcaaaaaca cgccaagaag aaaatccact tctattgatt ctagatggtc taaccgatcc
    12121 ccataatctg ggttctatct tgcgaacagc cgatgcgacc aatgtttcag gtgtcatcat
    12181 tcccaagcac cgtgctgtcg gagtaactcc tgtcgttgcc aaaacagcca caggtgctat
    12241 tgaacacgtt ccaattgccc gagtgacaaa cctcagtcaa accttagata aactcaagga
    12301 tgaaggcttc tggacctttg gaacggatat gaacggtact ccttgccaca agtggaatac
    12361 aaaagggaaa atcgccctca tcatcggaaa tgaaggaaaa ggcatctcta gcaacatcaa
    12421 aaaacaggtc gatgaaatga ttaccattcc gatgaatgga catgttcaaa gcctcaatgc
    12481 cagtgttgct gcagccattc tcatgtacga agtttttcta aatagactat aaaaaagttt
    12541 ccagtcatct gattggaaac ttttttatga ttaactatgt tctgtgataa acttgtaggc
    12601 ttcttgacca gcgatagctc catctccaac tgctgttgtt acttggcgaa ggtctttcaa
    12661 gcgaacatct ccaactgcaa agataccgtc gactgcagtc ttcatgtggc tatctgtcac
    12721 aatccagcct gcctgatctt ggatattcaa ttctttaaca aaatcgctaa gagggtccaa
    12781 accaacatag ataaagacac caccgaaggc ttgctctgtc acttgacctg ttttcacatt
    12841 ttcaaaaacg acagattcta ctctgttttc acccttgatt tccttcacta cagaatccca
    12901 gataaagctg attttttcat tcgcaaaggc gcgatcttgt aaaacctttt gggcacgaag
    12961 ttggtcacga cggtgaacaa tggtaacagt cttagcaaaa cgagtcaaga agagggcttc
    13021 ttcaacagct gaatctccac caccaactac caataaatct tggtcacgga agaaagcacc
    13081 atcacacaca gcacagtaag aaacaccacg actgttcagt tcttcttctc caggcactcc
    13141 caaaggacgg tgtttagaac cagttgctac gataactgta cgtgtttcat atgtttggtc
    13201 atcagtcatc actttcttaa aatcaccatg gtcttcgaca ttttcaacat aaccataaat
    13261 gtgctcaaca ccaagatttt caagtggttc aaacatcttt tcagccaatt caggtccact
    13321 aatattagcg tatcctgggt aattttcgat atcagatgta ttattcatct gaccacctgg
    13381 cagaccacct tcaatcaaag ctacttttag attgcttcga gcagcataca aggtcgcagt
    13441 catccctgca ggtccagcac cgataataat agtatcgtac atatagattc cttctttctt
    13501 gttgtaacta tctttattct aactctttct tgtcaatcac gcaaggatta tgccttgaaa
    13561 acaagaatta aactcataac cgcaaaggat aatactggga caaccaagac tccaatcata
    13621 atcatatcat agagatgggc ttggcgagcc ctggctgtct tatcaactcc cgacatggct
    13681 ctcagaccaa tccaaatccc taaaaaaatc agaacaagga tggtggtcaa gatcaaactc
    13741 tcgaaatata aagaaaatag ttgcagtagc atgatttctc tcatttctat cttttttaaa
    13801 gagtaaactc agctagtcca actaactgag ttttccttta tctattatat caaatataag
    13861 tccgtttgta actagcgaag aattcttttg tccgctcttc tttaggggtg tggataatct
    13921 catccggagt tccagactcg atgattttcc ccttatctaa gaagagaatt ttatccgcaa
    13981 cttgggctac aaaggacatg tcatgactga ccaaaatcat ggtctgacct gacttagcag
    14041 catctgcaat agacttttct acttcaccga ccaattctgg gtcaagggct gaagttggtt
    14101 cgtctaagag caaaacatct ggtttcatag caagcgcacg cgctagggca acccgttgct
    14161 tctgtccacc tgataaatgg cgaggataat ggttttcacg gtccgaaagc ccaaccttag
    14221 ccaactcttc cttggcaatc ttagtcgctt cttggtcaga taatttcttg acaacaacca
    14281 agccttcttt cacattatca agtgctgttc gacgttcaaa taaattaaac tgttggaaaa
    14341 ccatagacaa cttacggcgt agggcaagga tttcttcttg agtgatttta gaaaaatcaa
    14401 ctgaaaaacc atcaatctga atagagccac tgtcaggtgt ttcaagataa ttgagactgc
    14461 gaagaaaggt tgattttcca gctcctgaag aaccaatcaa ggctacaact tccccttttt
    14521 gaatatccaa gttcagatga tccaagacag tctgtcctga aaaggatttg cttaaattcg
    14581 aaatcttaat cattaacgaa ggtctccttt cacatctgtt tgcactgtat caggtgcaga
    14641 aatagccatt tttctctcga tgaaacgacc gaggctttca attccgatat tgactaccca
    14701 ataaacaagg gcaacagaga tgaagcgttc aaaatagcga taatcagctc cacctagaat
    14761 ctgagcttgg gcaaagactt ccacaacacc cgcactaaaa gctagagatg ttcccttggt
    14821 caaaccgatg agggaattaa tcaaggttgg agtagctacc accgctgcat taggaataat
    14881 cactcgtcga taaacttgcg ctcgggtcat acccagactg cgcgccgcct caatctcacc
    14941 aggattaact gagagaatgg ctgcacgaat ggtttcacta gcataagctg cctcattaaa
    15001 ggcaaaagcg acaatcgcaa aagctgcagc tggaatcgca ttgatattga gaccagttcc
    15061 ccattgctga ttgagggctt tcaaagccaa aggaattccg tagtaggtca acatgagttg
    15121 caccaaaatc ggtgtccctt ttaagaaact aacaaagaag gcctgcaagg gatataaaat
    15181 cttgacacga ttgatcttca caatggcaaa aagaagcgcc aaaaccaagc caaaaagggc
    15241 accaccaatt gtcaacataa ttgttgttgg aagttgttgc acaattctag ggattccatc
    15301 aaagaccgaa cgtaggctaa acagcttgcc atccggaatc aattgcatca agttttggta
    15361 ccaatctgat gctaaaatcg ttgtaacatt cataaaaaca tcctcttctg ataatgattc
    15421 attctaccat gcttctgccg tcaatgaaat tatttgctac ggtcagatac agactttgtc
    15481 tacctactag tttatcatac tttgaaacag ggaagttata tattttatct atcaaagaga
    15541 tagataaaaa ctatttcagt cccagttctt cataagattt tcgataaccg atttgcttaa
    15601 cagttccatt ttctactaaa atgggccgtt ttaacaacat accgtcactt gctagcaact
    15661 cagccgcttc ttggtttgac aaacttccta ccttatcttt tagccctaat tcacggtatt
    15721 tgataccact ggtgttgaaa aattgcttca attcaaatcc tgaggtttct agccaattca
    15781 aaatgacttc ttggctaggt gtttcttcca cgatatggac ggctttatag tccacaccta
    15841 attgatttaa ttcttgtttt gcttttttac aagttgaaca tttggggtat tcgataaatt
    15901 ctaacatgtg cttctccttc tatttgttta tatctttaaa atctacgcct tcatgctcca
    15961 agagccaagc tttcttttcc actcctgcag catagcctgt cagacgcttg cctgctccca
    16021 acacacgatg acaaggtact aggatagacc aaggattgcg tcccactgct ccaccaattg
    16081 cttgagcaga agccacttgc aggtcttgag caatttgtcc ataggtcact gtctgaccat
    16141 aaggaatgcc ctgtaaatag tcccaaactc tcttttcaaa attcgttccg attggcgcca
    16201 agagcaagtt ggataaatcc tgaggcttgc ctttaaagta atcatctaag caagcaataa
    16261 ctgggtctaa aataggatga ctaacaactt cttctatcgt ttcatctcct agtcccctct
    16321 caaaatgctt ctgctcctga acccaaattc catacaaata atggtcatca gctacaagtg
    16381 atagaatacc aattggtgag gagtagagga tttttacata cttcttttgc attatttctt
    16441 taatttttga taggccaagc gttcgccatc tactggcacc ttaccaactt gtttaaaacc
    16501 aagtttttca aaaatatgtt gcataacctt gttttcagca tgcgtatctg agcgaaaatc
    16561 aagataatca aaaccttcaa tcaagccctc taagaaggtt tgagcaactc cttttccctg
    16621 cacatctgct gccacagcaa tacggtgaaa gactagatac tctgactctc cagcttgcca
    16681 gtttccctca taaatagctt cataggcctc ctctggactc ttggtcacag cagcataggc
    16741 tagcagttct ccctcttcca aggctacgta ggcttgccct gagatgatat catcgataat
    16801 aacatctgca tttggatagc cattttgcca ctggttacta ccagcatctg ctaaacattt
    16861 tttggcatcc tctatcacct gcatgatggc atctacttca tttggaaaag ctaaacgaat
    16921 ttccatcttt tttcctcatt tctgactagt ttctcccatc atagcataat ttaagtcttt
    16981 ctacaagcag ttaaaacttg actttgtatt tttgttattt tataatctag ttatcaaaac
    17041 tatataaaaa ggagttgaaa atgaaaacta ccttttccta cccaaaatgg gcagaaattc
    17101 caaacattaa cctctatctg gaccaggttt tgctctatgt caatcaggtc tgcgccccta
    17161 tctctcctaa taaagacaaa ggcctaacag catctatggt caacaattat gtgaaaaatg
    17221 gttacctgac aaagcctgac aaaaaaaaat accaacgcca acagattgcc cgtttgattg
    17281 ctatcacaac cctcaagtct gtattttcta ttcaagaaat agcccagaca cttaatactc
    17341 tacaaactca agcaagttca gaccaactct acgatgcttt tgtggactac atgaaccaag
    17401 gaattgaccc agctaaccct attatccaaa ccagctgcca aaccgttaaa ctctatcatc
    17461 aaactctaga cttaatcgat catactcaag aggaggtaat ccaatgaaca ctagtcttaa
    17521 actcagcaaa caactcagtt ttggagagga gattgctaat agcgtgaccc atgctgtggg
    17581 tgcagtcatc atgcttatct tgctgcctat ttcatccatc tatagttatg aagcacacgg
    17641 atttttatcc tctatcggcg tttccatttt cgtcatcagt ctctttctca tgttcctatc
    17701 atccaccatt tatcactcta tggcctatgg ttcgacccac aaatatgttt tgcgaatcat
    17761 tgaccattct atgatttacg ttgccattgc cggctcatac acgcccgttg tcttgacctt
    17821 gatgaataac tggtttggct atctgattat tgtcatccaa tggggaacga ccatctttgg
    17881 tattctctat aaaatctttg ctaaaaaggt caatgagaaa tttagccttg ctctttacct
    17941 gattatgggc tggttggttc tggctatcat tcctgccatt atcagtcaaa cgacacccgt
    18001 tttctggagt ctcatggtaa ctggcgggct ctgttataca gttggagctg gattttatgc
    18061 caagaaaaaa ccttatttcc acatgatttg gcatctcttt atcctagctg cgtccgcact
    18121 tcaatacatc gctattgttt attacatgta aaaaagttga gaaattcaat ctcaactttt
    18181 ttctttacac atattgataa agtactggtg caagcgcaca tcatcagtca attctggatg
    18241 aaaagaactt accaacatat ttttttcttg ggctgcaaca atttgattgt tcactgttgc
    18301 taaaatttct acaccctcac caacactact gataatcgga ccacggataa aggtcattgg
    18361 aatcttgcca actcccttac attctgcttc cgtgtagaaa cttcctaatt ggcgcccata
    18421 agcattacgc tcgaccacca tatccatagt tcctagatga ctctctttct gagaagtgat
    18481 ttccttagcc agcaaaatta agcccgcaca ggtcccaaac actggtaagc cagatagaat
    18541 ggcttctcgg atgggaagta gcatgttctg gtcacgtaag agcttgccca tggttgtaga
    18601 ctcaccacca ggcaaaatca aacccgacaa gtcactctga tcttgctgaa aatcatctag
    18661 atttctgagt tctacactct cgacacctaa ttgatctagc acttttgcat gttctgcaaa
    18721 ggccccttgc aaggccaata ttccgatttt catctatttt cctcgttcag ccatgagaat
    18781 ttggatttca ttttcattaa taccaaccat ggcttctcct aaatcttcag agatttgagc
    18841 taggatttga ggattacgga agttagtcac agccttaaca atggcactcg ctcgtttaac
    18901 aggatctcct gacttgaaaa tacctgaacc gacaaagacc ccctctgccc ctaattgcat
    18961 cattaacgca gcatctgctg gcgttgcaac acctccagca gcgaaattta caactggcaa
    19021 ttttccatgt tcatgaacat attggaccaa ttctacaggg acttgcaaat ccttggcagc
    19081 aacataaagc tcgtcctcac gtaagttttg aatgcggcga atttcctgat tcatcatacg
    19141 catatgacga acagcttgga ctatatcccc tgtccctggt tctcctttgg tacgaatcat
    19201 ggaagcacct tcagcgatac gacgcaaggc ttcacccaaa tccttagcac cacagacaaa
    19261 aggaacttgg aattctttct tgtccacatg gaaacggtca tcagctggag atagaacttc
    19321 actctcgtcg atataatcaa tttcaatagc ctctaaaatc tgagcttcaa caaaatgccc
    19381 gattctgacc ttagccatta ctggaatact aaccgcttct tggatttcct taatcatctt
    19441 tgggtcgctc atgcgggaaa ctcctccagc tgcacgaata tcagccggaa ttcgttccaa
    19501 ggccatcaca gctgccgcac cagcagcttc tgcgatacga gcctgttcag gattctgcac
    19561 atccataata acaccaccct tgagcatctg tgccaagttt ttatttagtt cataacgatt
    19621 ttcagtcatt tgttttatcc tcatcatttg tattggtagc taccttttca ttttaagtca
    19681 aaacagaatg aagcacaaga taaaaaaact ataattgtat ggggacagat tagctaaaaa
    19741 ctatctgacc ttaacttaag gccagatagc tcattcattt ttatttttca gccgtaaggg
    19801 cagccattgt gatgtagttg tatggtttgt tgaagtgtgg caagaagaag aggtctgtca
    19861 atgccaattt atcaattgtc acatgctctt ggatagcaag tgagaacatg tggattccca
    19921 tgctaattgc aatatcatgt gaaaccattt gggcaccaag aatttcacgg ctatctttgt
    19981 caaagacaat cttaattgct acttcatggt tgtcatgttt catgaattct ggtttttgaa
    20041 gatcgttaaa gcctgtttca gttgcgttgt aaccagcagc tttcgctttt tcaagagtca
    20101 aaccagttga aaccatgtga agaccgtaga ttgagatacc atttgaacct tgaacaccga
    20161 ttccttccaa ttcatgtcca caagcattgt aggcaccaac gataccagtg cgaacagcat
    20221 ttgaagcaag agcgatatag cttgtatctt tacgagcatt gtcataaaca gtcgcacagt
    20281 caccaacagc gtaaacgcct gggatagatg tttcttgttt cttgtctaca aggaaggcac
    20341 cgttgcggaa gagttcgatc ttaccatctg caagggctgt gtttggacgg aaaccaactg
    20401 caaggataac catatccacg tcaaagcttt ctttgtcagt aatcaagcgt tcaactttac
    20461 cgtcaccttc gattgcttta acagtttgac ctagagccaa gcggatgttg tgatcttcca
    20521 agttcttcgc catcatttgt gtgaagtctt tgtcatagta accgttcaag acagtatcaa
    20581 cgatatcaac aaggacaact tcttttccaa gacgttcaaa ggcttcagca agttcaacac
    20641 cgatgtaacc accaccaaca acggcgatac ggtcgaggtg ttggctcttg tcagaaagtt
    20701 tattgataac ttcttcagca ttttggtaca atttcacgaa ttgtacgttt tcaagagttg
    20761 ctttaaattc gcggtttcct ttaacaattt caacaccttc gattggtggc aagattggtg
    20821 tagaacctgt agcgaaaatc aatttttcgt atgattcttt gtgctctttt ccttcaactt
    20881 ccgctgtaac tactttgtta tcatagtcga ttgaaagaac aggtgagttc atgtaaactt
    20941 tagcaccttt agcttccaat ttttctttat cagaatagaa caagccttca gcaccgtcaa
    21001 tttgttcacc aatccaaaga gccattccac atcctaggaa agagatgtta gagttttggt
    21061 caaatacaac aatttcgttc tcatttccaa aattatccaa catggtattg atacatgctg
    21121 taccagcgtg gttagcaccg actacaacga ttttactcat aaaaatttcc tgccttcatt
    21181 ctaaatttac cttactagta taccatttac cgttagcgtt tacaaggggg gtactattat
    21241 ttcagcattt tttatcaaaa ataattcccc atttatcctg ttttcaagcc tcatgaattt
    21301 tatgagcata tttcttgact ttttccaaat tttgtttgtg aaaattctaa ctttatggta
    21361 ttataataac atgaaaatga aatgtaaggg cttcaattta gatgattatt tgatattaca
    21421 acttattttc tataagagaa aggagttggt agcttgactc ttcgttcaca ttctgaacgg
    21481 ctaatgggga ctactatcac tatttcatta gtggatgagc aagccgatat ctttctccaa
    21541 aaatcctttg acttgctcaa agagcttgaa taccgcttca acgccaatag tcaagaatct
    21601 gagttgatgg aaatcaatta tcaagcagga atatcccccg tcacggttca tccagacctg
    21661 tttgagctga tttcacttgg gttagagcat agcctagcgc tctctagcca cctcaatatc
    21721 agcattggtc ccttaattca aacctggcgt atcggttttt cagatgccaa ggtcgcccag
    21781 cctcaagaaa ttgaatcggt gctgcctcta atcaatcctc atggtatcga gttagattct
    21841 tctacttcta ctgtgttttt aaaacagaaa ggaatgaaga tcgatctagg ttgtttagcc
    21901 aaaggataca gtgcggataa ggttgcccaa tttcttagga aagagggggt gacttctgcc
    21961 ttgatcaatc tgggagggaa tatcctgacc attggaaaaa atcaggcaag aggggataac
    22021 ccatggcaaa tcgggattca agacccagcc aatcctaggg gaaatcattt aatgaccatc
    22081 cctgttgtca ataaatctgt cgtgacttca ggcatttatg aacgtcacct gaccgtcgat
    22141 ggacaagatt accatcacat ttttgacagt caaacaggat atcctgttga aacggaacta
    22201 gcgagtctaa caatcatctc tgataaatca gtcgatggcg aaatctggac gactcgactc
    22261 tttggagaaa gacctgcttc tatcctctgg caagtcgaaa gtttggaggg catcgaagtc
    22321 atcctcatcg ataaagaagg ccacctaagc tgttcttcag gaattcctac tctatagaga
    22381 caactgtttc aatggaattt taaaattgta ttatgtaaaa agagaaagga aatatcatgt
    22441 taaaacttat tgctattgtt ggaacaaatt caaaacgttc tacaaaccgt caattgcttc
    22501 aatacatgca aaaacacttt gctgacaaag ctgaaattga acttgttgaa atcaagacca
    22561 ttcctgtctt caacaaacca gctgacaagc aagtacctgc tgaaatattg gaaattgctg
    22621 ctaaaatcga agaggcagat ggcgttatta tcggtactcc tgagtatgat cactctattc
    22681 cagctgtttt gatgagcgct cttgcttggt tgtcttatgg tatttaccca cttttgaaca
    22741 aaccaatcat gattacaggt gcttcttacg gtacgcttgg ttcatctcgt gcccaattgc
    22801 aacttcgtca aatcttgaat gctcctgaaa tcaaggcaaa tgttcttcca gatgaattct
    22861 tgctctcaca ctctcttcaa gcatttaacc caagtggcga cttggttgac cttgatgtta
    22921 tcaagaaatt ggatgccatc tttgatgact tccgtatctt cgtgaagatt actgagaaat
    22981 tgcgcaatgc gcaagaattg cttcgcaaag atgcagaaga atttgactgg gaaaatttgt
    23041 aagataggag accgaaagaa tgaaatttgt tggacttgtt ggatcaaact acgatcaatc
    23101 atataaccgc aaactcttgg aatttatccg tcgcaatttc aaattcaaat ttgaattaga
    23161 agtacttgaa atcgacgaag ttccaatgtt taaccaagac gaaaaatggg acgaaagctt
    23221 ccaattgcgt ttcttgtata acaagattac acgtgctgat ggtgtcatta tcgccactcc
    23281 tgagcacaac cacactatct cagcttcact caaatctgta cttgaatggc tttcatacga
    23341 agttcatcca tttgaaaaca agcctgttat gattgtgggt gcatcatact atgaccaagg
    23401 aacatcacgt gctcaagttc accttcgtaa aatccttgat gctccaggtg ttaatgccta
    23461 cacgcttcca ggtaacgaat tccttcttgg taaagctaag gaagcttttg ataacaacgg
    23521 aaatatcacc aacgaaggca ctgttaaatt ccttgaaact tgcttagata actttgttaa
    23581 atacgtagga gtcgtttcga aattgaaaaa accaaaacca atcgaatcag aagacttgga
    23641 ttgtggaaaa ccaatcgcta caaccatcac agaagttgac cctgacgatc cagaatgggt
    23701 agaaaaagtt gcagcaatca ctggtgctgt ttctggtgat acctatgtca aattggacca
    23761 cggtatccta acagttaacc aaattgatat gttcttgaaa gctatgccat ttgaattgac
    23821 atacgctgat gacaacaacc aattcctcta ctacaacaac gctcaccaag atccagacac
    23881 tatgttcgct aaacgtgtac cacctcaatc aggtagccgt atgtcgactg ttcatggttc
    23941 tcttccacca gcacgtatga agaacgtaga gtgggttatc ggaacacttc gcaacggaaa
    24001 ccaagaatac gtccgtacga tcgttccagg ttctcctgca ggtgttatca acactcacaa
    24061 ctaccaagca atgtactatc ctgatggatc atacgctggt atcaatgaaa tcgtctttaa
    24121 cttccaacca tggcttgact ggtacctaaa agagactggt caacgtttgg tgggtggtag
    24181 cggaccattt gctcctgctg ccggaggtca tggaaacgcc gatgctactt ctggcgcttc
    24241 tgattcaggg gatgctggag gccacggtgg tgacgcagac gctacagccg gtgcaagtta
    24301 ctaatgatag tttttaggaa ccattatggt tcctttttta gcgaaaaaag actaacaaac
    24361 acaaactgct agtctctttg ttatcgtttc atctatcctt gttaaaagaa agaatactta
    24421 tactcaatga aaatcaaaga gcaaactagg aagctagccg caggctgtac ttaagtacgg
    24481 caaggtgaag ctgacgtggt ttgaatttga ttttcgaaga gtattattat gaatttgggt
    24541 catcaagact acggccatgt aatccttttt cacgttgtac ttggcgtagt ttttctggtg
    24601 taacatcgtt tccttcttcg tccacaattt tgattccttc aatgttgtga cgaacagcgc
    24661 ggcgataacc ttcgatgtac tcctcacgta gtttggcttg ttccattttt tcttctggtg
    24721 ttaagccttc tgtttttttc tttttagcaa gctcattgat acgagcaatt tttttcggat
    24781 ccatttcttc tcctttgtaa taagataaag tccgtttatc aaattttatt tagtgttaat
    24841 ttggttaaaa caagcaaact tagctgcttt cttggttagt tgtgacaaga ttgccaaacg
    24901 attttgacgg acagcctgat cttcagccat taccatagta ttttcaaaga aagcatcaat
    24961 gactgggcta agcgcaaaga gttgtttcaa ttgctgactt gcaggtcctg ataaaacgag
    25021 tgtttctact gcttctgcca aagctttttc ttggtcattc tcaaatagtg ctgaatcaac
    25081 tgtagcaacc ccttctgcct tctcagccag gttaaaggca cgagaaagtg attcaacaga
    25141 tggtttaaaa tattcttcct tgcttacttc tacgagagca cttgctgctt ccaacatatc
    25201 tgccacaaca aagtttgaac ctgcaagaac tgcttccttg atatcttttg gagtagagcc
    25261 catcatctta tcaacacgag ccttgataaa gtccataacc tctgctttat tttcataagt
    25321 caaactgtca aatttcaatg cataaaggct atcaatcagc tcatccatag caatgtgcca
    25381 accaaaggca tccaagatac gaaccacacc ttgagttgca cgacgaaggg cataagggtc
    25441 attagaacct gatggaatca atcctactga gaagaaactc aaaatcgtat ccaatttgtc
    25501 tgcaatggct agaactgcgc cgaccttgct ctctggaagt tctccttcag ctgatgtagg
    25561 catgtagtgt tcacgaatag cagctgccac cgctggagtt tcaccagcaa gaagggtgta
    25621 tttttcaccc ataattcctt ggagttcgtc aaattcacca accatacctg tcaataagtc
    25681 aaacttgtaa atggctgctg cacgagcaag gtcaactgtt tcatccactg acaaaccagc
    25741 tttttctgcc aaaagtacag tgatttgacc cgtacgaatc atgtgttcac gaagggaacc
    25801 aatcttctca tggaaggtga cattgtttaa tttttcaaca agatctgaaa tcaccaattt
    25861 ttggtcttca cgccagaaga attctccgtc ttccaagcgg gctaccaaga ctttttcatt
    25921 tcctttaatg acatttttca aacgctctgc gtttccgtta cgaacagaaa tgaagtttgg
    25981 caagagtttt ccatcttgat cacgaacaac aaagtaacgc tggtgttcct tcatagaagt
    26041 caccaagact tcttctggaa cttcaaggta tttagcatca aaacttccca tgaaggcagt
    26101 tgggtattca accaaattca agacttcatt cagcaaatcc gcatcaattt cgatacgtac
    26161 accatgtttt gcctcaattt ccttgatttg gtcaacaatc atttgctcac gttcacatgg
    26221 atctgcgatt acaaactgct tacgaaggtc ttcttcatag ctcaatgctg actgaatttt
    26281 ggtttctttc cccaagaaac gatggccacg gctcacacga cttcccttga tatcaaggaa
    26341 atccaagtca aactcttgct catccaagag aacagttaaa gtgtggacag gacgaatgta
    26401 ttcaaagcta tttccagccc agtgcatgct gacagggaaa gtcaatgact tcaagacatc
    26461 tacaacacca ggaacaatgg cttcaactgc ttgaccaact tcttccttag tgacatagac
    26521 atattcttca cccttgattt cacggaattc gatatcttca acagtcaagc cttttccacg
    26581 gacaaatcct tgagctgctt tggtaaagtt tccatcacta tccaaggcaa ttttctttgc
    26641 tggtcccttg aaatcttctg tcaaatcaga ctgtttgtct gataaaccag tcacacgaac
    26701 agccaaacga cgtggtgttg agaaggtttg aatggcttca aaagacaggc ggttttcctt
    26761 gaggaaggct gccatttttt cgcctagttg tttttcactt ggtgtgacaa cataggctgg
    26821 taattcttca agaccgagtt ctactaataa gttttttgtc atgttttttc ctttacattt
    26881 tcttcaatct tttttgatat gcaccttagg tactggggac gtcgctaact aactctgtga
    26941 gtctgtatgg aaatccaatg ctaaatttat caagatttcc aacaatctca tcaaagtgga
    27001 tttagctgac tgatttttga acctaaggtt tcaaaaatcc ccacataaca gtacggcggt
    27061 gcatatccct ctagcaagtc ttcgacttgc ctctaacgat tttagagtac agtattactc
    27121 actattctgc gtcttctgct aaaagtttaa ctcgtgtttc ttcatccaaa agtgggtaac
    27181 ctaggcgttt gcgttctgca acaaaggttt tggctacaac acgggccaag ttacggatac
    27241 gagcgatata gcctgcacgc tctgttacag atacggcacc acgcgcgtca agcagattaa
    27301 aggtatgtga acatttgaga acatagtcat aggcagggtg taccaagcct tcttctaatg
    27361 cacgaccagc ttctttttca aacttatcaa agttttcaag caaaatttct tggtccgaaa
    27421 tttcaaatga atattttgag tgctcatact caggctggat aaagatttct ccgtatttta
    27481 caccatcagc ccactcgata tcatagacag agtctacttc ttgaatgtaa gaagccaagc
    27541 gctccaaacc ataggtaact tccgcagtca cagggccagt tgccaatcca ccgacttgtt
    27601 ggaaataagt gaactgagtg atttccattc cgtcaagcca aacttcccaa ccaagaccag
    27661 ctgaaccagt tgatgggttt tcccagttgt cctcaacaaa acgaatatcg tgctccaaag
    27721 gattgattcc caatttttcc aaagactcaa ggtaaagttc ttggatattt gatggagaag
    27781 gcttcatgac cacctggaat tggtggtgtt ggtagagacg gttagggttt tccccataac
    27841 gaccgtcagc aggacgacgt gatggctcta cataagctgc attccatggc tcaggtccga
    27901 tagcacgaag gaaagtgtaa ggactcattg tccccgcacc tttttcattg tcataagcct
    27961 gcataagcat acaaccttgg tcattccaaa attgttgcaa agtcaaaata atttcttgaa
    28021 atgttaattt cttagacatt gtttactcct taataaaaaa tgatttcttg cgacacgagt
    28081 ctgcctcgtc gattatacga ggcaagacga gttagtactg cgtctagctc agcaccctag
    28141 tgctgagcgt ggggcagtcc gactgtaagg aggtttctgc cactgatgca gtgaaatgtt
    28201 aatttcttag acattgttta ctccttaata aaaaataatt tcttgcga
//
LOCUS       DAVBDG010000025        26518 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBDG010000025 DAVBDG010000000
VERSION     DAVBDG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.025"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(418..510)
                     /locus_tag="VQ526_001044"
     CDS             complement(418..510)
                     /locus_tag="VQ526_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2554778.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            671..1642
                     /gene="ruvB"
                     /locus_tag="VQ526_001045"
     CDS             671..1642
                     /gene="ruvB"
                     /locus_tag="VQ526_001045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2554779.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1623..2195
                     /locus_tag="VQ526_001046"
     CDS             1623..2195
                     /locus_tag="VQ526_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2554780.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2426..3184
                     /locus_tag="VQ526_001047"
     CDS             2426..3184
                     /locus_tag="VQ526_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2554781.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKIITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3193..3996
                     /locus_tag="VQ526_001048"
     CDS             3193..3996
                     /locus_tag="VQ526_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2554782.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4018..5277
                     /gene="rseP"
                     /locus_tag="VQ526_001049"
     CDS             4018..5277
                     /gene="rseP"
                     /locus_tag="VQ526_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2554783.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5290..7143
                     /locus_tag="VQ526_001050"
     CDS             5290..7143
                     /locus_tag="VQ526_001050"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2554784.1"
                     /translation="MKQSKIPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7242..8621
                     /gene="bglA"
                     /locus_tag="VQ526_001051"
     CDS             7242..8621
                     /gene="bglA"
                     /locus_tag="VQ526_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2554785.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            8814..10622
                     /gene="glmS"
                     /locus_tag="VQ526_001052"
     CDS             8814..10622
                     /gene="glmS"
                     /locus_tag="VQ526_001052"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2554786.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            10894..11943
                     /locus_tag="VQ526_001053"
     CDS             10894..11943
                     /locus_tag="VQ526_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2554787.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(12043..15813)
                     /gene="spuA"
                     /locus_tag="VQ526_001054"
     CDS             complement(12043..15813)
                     /gene="spuA"
                     /locus_tag="VQ526_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2554788.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVQNESV
                     ENSSKKNIPVTPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            16230..16643
                     /gene="rpsL"
                     /locus_tag="VQ526_001055"
     CDS             16230..16643
                     /gene="rpsL"
                     /locus_tag="VQ526_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2554789.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16663..17133
                     /gene="rpsG"
                     /locus_tag="VQ526_001056"
     CDS             16663..17133
                     /gene="rpsG"
                     /locus_tag="VQ526_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2554790.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17558..19639
                     /gene="fusA"
                     /locus_tag="VQ526_001057"
     CDS             17558..19639
                     /gene="fusA"
                     /locus_tag="VQ526_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2554791.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19743..24134
                     /locus_tag="VQ526_001058"
     CDS             19743..24134
                     /locus_tag="VQ526_001058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2554792.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEVTYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFINKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24236..24499
                     /locus_tag="VQ526_001059"
     CDS             24236..24499
                     /locus_tag="VQ526_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2554793.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24492..24770
                     /locus_tag="VQ526_001060"
     CDS             24492..24770
                     /locus_tag="VQ526_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2554794.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24791..24943
                     /locus_tag="VQ526_001061"
     CDS             24791..24943
                     /locus_tag="VQ526_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554795.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            24965..26206
                     /locus_tag="VQ526_001062"
     CDS             24965..26206
                     /locus_tag="VQ526_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2554796.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26216..26446
                     /locus_tag="VQ526_001063"
     CDS             26216..26446
                     /locus_tag="VQ526_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2554797.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
BASE COUNT         7454 a         5076 c         6246 g         7742 t
ORIGIN      
        1 atttttcgga ttttggtcgg ggaattggcg gggacttttt tagcgaatat aactaagaaa
       61 taggtctgtg gtcgcttcag ctagttcaaa ctcaatttga ttgtaagtga ctgttaattg
      121 aagaactgga actactgtaa taatgatttt gcttgtctag tagtggcgta ttaaatgaaa
      181 agtgacatgt aagattgtct cttcgtttac gaaatcaaaa tttctatgaa attgatagaa
      241 tccctaacgt tctcttaatc ttaaccgtct tgtcgtacag cggttgaaga aaatgtaaac
      301 ctttgacaaa attcgtgaac tatgggataa taaaaaggaa ttgagtacta gttctatatc
      361 ataggctaga aaagacccca agctcacgac caaataagct tgaggtcttt tttgacacta
      421 tttgtccttg tttagccatt tatcgactat acgaagaacg acaccgacca caattggtcc
      481 gatgatagtt ttgagtacta attccatcat gggctatctc acctcctttc gtaggcggtg
      541 tagaagtgtc gtagaatatt ataccacata agtgctaaac ttaggagtca cccaatcagg
      601 tggctttttt gtttgtggct tggttttttg atataataga gccatgagta gaattttaga
      661 taatgagata atgggggatg aggagttagt agaacgcacg ctccgtcctc agtatttacg
      721 tgaatatatc ggacaggata aggtcaagga ccagctacaa atctttattg aagctgccaa
      781 aatgcgggat gaagcgctgg atcatgtgct cttatttggg cctccaggtc tcgggaaaac
      841 gaccatggcc tttgttattg ccaacgaact gggagtcaat cttaagcaga cgtcgggtcc
      901 agtcattgaa aaagccggag atctggtagc tattttgaat gagttagagc ctggggatgt
      961 actttttatt gatgagatcc atcgtttgcc aatgtcagtg gaagaggtgc tttatagtgc
     1021 tatggaggac ttctacatag atattatgat tggggctggt gagggtagtc gtagtgttca
     1081 tttggagtta ccacctttta ccttgattgg tgcgacgact cgggctggta tgctctccaa
     1141 tccgctacgg gcacgttttg ggattacagg ccatatggag tattatgccc atgctgactt
     1201 gacagaaatt gtcgagcgga cggcagatat ttttgagatg gaaatcactc atgaggcagc
     1261 atctgagttg gccctacgta gtcgtgggac ccctcgtatt gccaatcgtc tcctcaagcg
     1321 cgtgcgcgat tttgcccaga taatggggaa tggggtaatt gatgatatta ttaccgataa
     1381 ggctttgact atgctggatg ttgaccatga aggtttggac tatgtggatc aaaaaatcct
     1441 tcgtaccatg attgagatgt acagtggagg acctgttggt ctaggaactc tttctgtgaa
     1501 tatcgccgaa gagcgtgaga cagttgaaga tatgtatgag ccttacttga ttcaaaaagg
     1561 ttttatcatg cggacacggt ctggacgggt ggcgactgct aaggcatatg agcacttagg
     1621 ttatgaatac agtgaaaaat aagcaagaaa tcttagaggc ttttagagaa aatccagata
     1681 tgatggccat tctgacgatc atccgagacc ttggtctgaa agactcgtgg ttggcagcag
     1741 gttctgtcag aaatttcatt tggaatcttt tgtcagacaa atcccctttt gatcatgaaa
     1801 cagatataga tgtgattttc tttgatccag atttttctta tgaggaaacc ttattactgg
     1861 agaaaaagct gcgagaggat tttcctcagt accagtggga attgaaaaat caggtctata
     1921 tgcatcagca cagtcctcac actgcttcct ataccagttc tcgtgatgct atgagtaagt
     1981 atccagaacg gtgtacgaca gttggactgc gcttgaatga agaatcagat tttgaactct
     2041 atgcacctta tggtttggag gatattttga attttcaagt tcgtccaact cctcatttct
     2101 tagaaaatga agaccgaatg gaactctatc aaacacgttt atccaagaaa aattggcagg
     2161 agaaatggaa aaatttgatt tttaaaaata cttaaggaaa ctttaagcta gggagtgtac
     2221 actaagttca taagttaaga agaccttaac ttaaactcct aaaacttttt cataataatc
     2281 tccctataaa aataaagtcg cccaatcagg cggcttattt ttttgaaaaa tgggcttggt
     2341 gcctgagaat aaatagctta gtgatagaag aaaatgggga aatatggtat aataaaacga
     2401 tagatttttg aataggaata agatcatgtt tggatttttt aagaaagata aggctgtgga
     2461 agtagaggtt ccgacacagg ttcctgctca tatcggcatc atcatggatg gcaatggccg
     2521 ttgggctaaa aaacgtatgc aaccgcgagt ttttggacat aaggcgggca tggaagcatt
     2581 gcaaaccgtg accaaggcag ccaacaaact gggcgtcaag attattacgg tctatgcttt
     2641 ttctacggaa aactggaccc gtccagatca ggaagtcaag tttatcatga acttgccagt
     2701 agagttttat gataattatg tcccggaact acatgcgaat aatgttaaga ttcaaatgat
     2761 tggggagaca gaccgcctgc ctaagcaaac ctttgaagct ttaaccaagg ctgaggaatt
     2821 gactaagaac aacacaggat tgattcttaa ttttgctctt aactatggtg gacgtgctga
     2881 gattacacag gcgcttaagt tgatttccca ggatgtttta gatgccaaaa tcaacccagg
     2941 tgacatcaca gaggaattga ttggtaacta tctctttact cagcatttgc ctaaggactt
     3001 acgagaccca gacttgatta tccgtactag tggagaatta cgtttgagca atttccttcc
     3061 atggcaggga gcctatagtg agctttattt tacggacacc ttatggcctg attttgacga
     3121 agcggccttg caggaagcta ttcttgccta taatcgtcgt catcgccgat ttggaggagt
     3181 ttaggaggaa atatgaccca ggatttacag aaaagaacct tgtttgcagg gattgccctg
     3241 gctattttcc taccaatttt aatgattggg ggcctcttgc ttcagatagc aatcggaatc
     3301 atagccatgc tagccatgca tgaacttttg aagatgagag gtctagagac catgacgatg
     3361 gagggcctct tgaccctctt tgcaaccttt gcattgacca ttcccttgga gaattacctg
     3421 acttttttgc cagttgatgg gaatgtggtt gcctatagtg ttttgatttc aatcatgtta
     3481 ggaacgaccg tttttagcaa gtcttatacg attgaggatg cggttttccc tcttgctatg
     3541 agcttctacg tgggctttgg atttaatgct ttactagatg ctcgtgttgc aggtttggac
     3601 aaggctctct tagccttgtg tatcgtctgg gcgacagaca gtggtgccta tcttgttggg
     3661 atgaactatg ggaaacgaaa gttagcacca agggtatcgc ctaataaaac ccttgagggt
     3721 gccttgggtg gtattttagg agcaatttta gtaaccatta tctttatgat agttgacagt
     3781 acagttgctc ttccatatgg aatttacaag atgtcagtct ttgctatttt ctttagcatt
     3841 gctggacaat ttggtgattt actagaaagt tcgatcaaac gtcattttgg tgttaaggat
     3901 tctgggaaat ttatccctgg acatggtggt gttttggatc gtttcgatag tatgttgctt
     3961 gtatttccaa tcatgcactt atttggactc ttttaatcaa aagacggagg aaacgctatg
     4021 ctcggaattt taacctttat tctggttttc gggattattg tagtggtgca cgagttcggg
     4081 cacttctact ttgccaagaa atcagggatt ttagtacgtg aatttgccat cggtatggga
     4141 cctaaaattt ttgctcacat tggtaaggat ggaacggcct ataccattcg aatcttgcct
     4201 ctgggtggct atgtccgcat ggccggttgg ggtgatgata caactgaaat caagacagga
     4261 acgcctgtta gtttgacact tgctgatgat ggtaaggtta aacgcatcaa tctctcaggt
     4321 aaaaaattgg atcaaacagc cctccctatg caggtgaccc agtttgattt tgaagacaag
     4381 ctctttatca aaggattggt tctggaagaa gaaaaaacat ttgcagtgga tcacgatgca
     4441 acggttgtgg aagcagatgg tactgaggtt cggattgcac ctttagatgt tcaatatcaa
     4501 aatgcgacta tctggggcaa actgattacc aattttgcag gtcctatgaa caattttatc
     4561 ttaggtgtcg ttgttttttg ggttttaatc tttatgcagg gtggtgtcag agatgttgat
     4621 accaatcagt tccatatcat gccccaaggt gccttggcca aggtaggagt accagaaacg
     4681 gcacaaatta ccaagattgg ctcacatgag gttagcaact gggaaagctt gatccaagct
     4741 gtggaaacag aaaccaaaga taagacggca ccgactttgg atgtgactat ttctgaaaag
     4801 gggagtgaca aacaagtcac tgttacaccc gaagatagtc aaggtcgtta ccttctaggt
     4861 gttcaaccgg gggttaagtc agattttcta tccatgtttg taggtggttt tacaactgct
     4921 gctgactcag ctctccgaat tctctcagct ctgaaaaatc tgattttcca accggatttg
     4981 aacaagttgg gtggacctgt tgctatcttt aaggcaagta gtgatgctgc taaaaatgga
     5041 attgaaaata tcttgtactt cttggcaatg atttccatca atattgggat ttttaatctt
     5101 attccgattc cagccttgga tggtggtaag attgtgctca atatcctaga agccatccgc
     5161 cgcaaaccat tgaaacaaga aattgaaacc tatgtcacct tggccggagt ggtcatcatg
     5221 gttgtcttga tgattgctgt gacttggaat gacattatgc gactcttttt tagataatcg
     5281 aggaatatta tgaaacaaag taaaatacct atcccaacgc ttcgcgaaat gccaagcgat
     5341 gctcaagtta tcagccatgc tcttatgttg cgtgctggtt atgttcgcca agtttcagca
     5401 ggtgtttatt cttatctacc acttgccaac cgtgtgattg aaaaagctaa aaacatcatg
     5461 cgccaagaat tcgaaaagat tggtgctgtt gagatgttgg ctcctgccct tcttagtgca
     5521 gaattgtggc gtgaatcagg tcgttacgaa acctatggtg aagaccttta caaactaaaa
     5581 aaccgtgaaa aatcagactt tatcttaggt ccaactcacg aagaaacctt tacagctatt
     5641 gtccgtgatt ctgttaaatc ttacaagcaa ttgccactca acctttatca aattcagccc
     5701 aagtatcgtg atgaaaaacg cccacgtaat ggacttcttc gtacacgtga gtttatcatg
     5761 aaggatgctt atagtttcca cgctaactat gatagtttgg atagtgttta tgatgagtac
     5821 aaagcagcct atgagcgtat tttcactcgt agtggtttag acttcaaggc tattattggt
     5881 gacggtggag ccatgggtgg taaggatagc caagaattta tggccattac atctgctcgt
     5941 acagaccttg accgctgggt tgtcttggac aagtcagttg cctcatttga cgaaattcct
     6001 gcagaagtgc aagaagaaat caaggcagaa ttgctcaaat ggatagtctc tggtgaagat
     6061 accattgctt actcaagtga gtctagctat gcagctaact tagaaatggc aacaaacgag
     6121 tacaaaccaa gcaaccgtgt tgtcgctgaa gaagaagtta ctcgtgttgc aacgccagat
     6181 gttaaatcaa ttgatgaagt tgcagctttc ctcaatgttc cagaagaaca aacgattaaa
     6241 accctcttct acattgcaga tggtgagctt gttgcagccc ttctagttgg aaatgaccaa
     6301 ctcaacgaag tcaagttgaa aaaccactta ggagcagatt tctttgacgt tgctagcgaa
     6361 gaagaagtgg cgaatgttgt tcaagcagga tttggttcac ttggaccagt tggtttgcca
     6421 gagaatatta aaattattgc agatcgtaag gtgcaagatg ttcgcaatgc agttgtcggt
     6481 gctaacgaag atggctacca cttgactggt gtgaacccag gccgtgattt tacttcagaa
     6541 tatgtggata tccgtgaagt tcgtgagggt gaaatttccc cagatggaca aggtgtcctt
     6601 aattttgcgc gtggtattga gatcggtcat attttcaaac tcggaactcg ctattcagca
     6661 agcatgggag cagatgtctt ggatgaaaat ggtcgtgctg tgccaatcat catgggatgt
     6721 tacggtatcg gtgtcagccg tcttctttca gcagtgatgg agcaacacgc tcgcctcttt
     6781 gttaacaaaa cgccaaaagg tgaataccgt tacgcttggg gaatcaattt ccctaaagaa
     6841 ttggcaccat ttgatgtgca tttgattact gtcaatgtca aggatgaaga agcgcaagcc
     6901 ttgacagaaa aacttgaagc aagcttgatg ggagctggtt atgaagtctt gacagatgac
     6961 cgtaacgaac gtgtcggtgt taaatttagc gatagtgact tgattggtct tccaatccgt
     7021 atcactgttg gtaaaaaagc ggccgatggc attgtagaag ttaagatcaa ggcaactggt
     7081 gacaccattg aggttcatgc agataacgtg cttgaaacgc ttgaaatcct aagcaagaaa
     7141 taaaaactat aatcagaaga aaaacaagga aaaaatgtaa ctagttttta ccttgttttt
     7201 tctgtataat gggagaaatg agtagataaa gaggtaaatg tatgctaaga tttccaaagg
     7261 attttgtctg gggatcctct acttctggac cgcagacaga aggacgtgta gctggtgacg
     7321 gtaagggaga caatctctgg gattactggt tccaagtgga gccaaatcgt tactataatg
     7381 ggattggttc agataagaca tcgacttttt atgaaaattg ggagcgggat attgagcttt
     7441 tgttagagac tggtcacaca gcctttcgga cttctattca gtggtcacgg atttttccac
     7501 aaggctgtgg aaaagtcaac cctcaaggtg tggattttta tcgtaaggtc tttgaggcta
     7561 ttaaggctaa aggaattcgt ctgttagtca atctctatca ttttgattta ccttttgccc
     7621 ttcaagagga tggtgatggt tgggaaaata aggcgacagt ctcagcctat gaagactatg
     7681 ctcgtttttg ttttgagact tatggagatt tagtggatca atggattacc tttaacgagc
     7741 ccatcgttcc tgtagaattt ggttattttt atgatgccca ttatccacat aaggtggatg
     7801 cagaggcagc tgttaaggta gcctatcata cacaattggc cagcagccgg gctgttaagg
     7861 cttgccatga acttttgcct gattccaaga ttgggattgt cctcaacttg acaccggctt
     7921 atccacgtag ccagcatcct gctgatgtca aggcagctcg tattgcggcc ctttttcagg
     7981 cccaatcttt cttagatcca tctgtcttgg ggacttatcc acaggagttg gtagaaatct
     8041 tgcatgaaca cggtctttta cctgatgcta cagaggaaga gttggaactc attcgtgata
     8101 atacggtgga cttccttggt gtgaactact atcaaccttt gcgtgttatg gctcctcgat
     8161 ttgctaagca tccagagagt ccactcttac cagaacattt ttacgagcct tatgtgatgc
     8221 ctggacgtaa aatcaattct caccgtggtt gggagattta tgagcaaggg atttatgaca
     8281 tcgcccaaaa tatcaaggaa aattatggca atattgagtg gatgttgact gagaatggta
     8341 tgggtgttga aggggaagaa aaattccgtc aagatggaat gattcaagat gattaccgta
     8401 ttgactttgt aaaaggtcat cttcgtgaac ttcaccgtgc cattgaagat ggtgccaact
     8461 gtaagggcta cttgatttgg acctttattg actgctggtc atggctcaac agctataaaa
     8521 atcgctatgg tttggtcgaa ttagacttgg aaacgcaaga acgtcgtctg aagaaatcag
     8581 gccactggtt caaagaatta agcgataata atggatttta aaaaggactc tgactgatta
     8641 tcccaatcgg tcagagtttt tatgcttaca ggagcttaat ttgaactata ccaattttta
     8701 ctcttgacaa gtgaaagaaa caagtatata ctgtttttgc tgattctata aaagatgaat
     8761 tagactatac caattttaag gagaaagcac agcttgcctg tgtcgtatat actatgtgtg
     8821 gaattgttgg tgttgttgga aacacaaatg caactgatat tttgattcaa gggcttgaaa
     8881 agcttgaata ccgtggctat gattctgcgg gaatttttgt cctagatggt gctgataacc
     8941 atttggtgaa ggcggttggt cgtattgcag aattgtctgc caagacagct ggtgttgagg
     9001 gaacaactgg tatcggacat actcgttggg ctactcacgg aaaaccaact gaggacaatg
     9061 ctcacccaca ccgctctgag acagaacgct ttgtcttggt gcataatggg gtgattgaga
     9121 actatcttga aatcaaggaa gaataccttg caggtcacca cttcaagggg cagacagata
     9181 ctgaaatagc cgttcatttg attggaaaat ttgcggaaga agaagggctc tcagttcttg
     9241 aagccttcaa aaaagccctt cacatcatcc gtggttctta tgcctttgcc ttggttgact
     9301 cacaagatcc tgaagtcatc tacgtagcta aaaataaatc accacttttg attggtcttg
     9361 gagaaggcta taacatggtc tgctcagacg ccatggctat gattcgtgaa actaaccaat
     9421 acatggaaat ccatgaccaa gagttggtaa tcgtcaaggc tgatagtgtg gaagttcaag
     9481 actatgatgg aaatcgtcgt gagcgtgcta gctacactgc tgaacttgac ttgtcagata
     9541 tcggtaaggg aacttaccct tactacatgc tcaaggaaat tgacgagcaa ccaactgtta
     9601 tgcggaaact catccaagcc tacacagatg aggctggtca agttgtcgta gacccagata
     9661 tcattaaggc tgttcaagac gcagaccgca tctacatcct tgcagctgga acatcttacc
     9721 atgcaggatt tgcttctaag aaaatgttgg aagaattgac agatacacca gttgaacttg
     9781 gaatctcatc tgagtggggc tacggtatgc cacttctcag caagaaacca ctcttcatct
     9841 ttatcagcca atctggtgaa acagcggata gtcgtcaagt tttggtcaag gctaatgaaa
     9901 tgggaattcc aagcttaaca gtgacaaacg ttccaggttc aaccctctca cgtgaagcca
     9961 actataccat gctccttcac gcaggtcctg aaattgccgt ggcatcaact aaagcctata
    10021 cagcgcaaat cgcagccctt gccttccttg caaaagcagt cggagaagca aatggtaatg
    10081 ctaaagcgca agcctttgat ctggttcatg aattgtcaat cgtagctcag tctatcgaat
    10141 caactctttc agagaaagaa accattgaag ccaaggttcg tgaacttctt gaaacaactc
    10201 gtaacgcctt ttacatcgga cgtggtcaag attactacgt agccatggaa gcaagtctca
    10261 aactcaaaga gatttcttat atccagtgtg aaggctttgc tgcaggagaa ctcaagcacg
    10321 gaaccattgc cttgattgaa gaaggaacgc ctgttttggc tctcttgtca gatccagttc
    10381 ttgccaatca tactcgtgga aatatccaag aggtcgcagc ccgtggtgcc aaagtcctca
    10441 ctatcgcaga agagaatgta gccaaagata ccgacgatat cgtccttacg accgtacatc
    10501 catacctctc accaatttca atggtcgtac caacgcaatt agtcgcttac tttgcaaccc
    10561 tccaccgtgg cctcgatgtg gacaaaccac gtaaccttgc caagtcagta acggtagaat
    10621 aagctaaaaa agtctagtta tctaggcctt tttcttgtga gcaaattggt tgcttgtact
    10681 caagattcgt gttatactct tcgaaaatct cttcaaacca cgtcaacgtc gccttgccgt
    10741 aggtatggtt actgacttcg tcagttctat ccacaacctc aaaacactgt tttgagcagc
    10801 ctgcagctag cttcctagtt tgctctttga ttttcattga gtattagaat aatttttcaa
    10861 attgaagtac agagatagat aaggagaatc acaatggtag aattgggaat ttcaacattt
    10921 ggggaaataa cggagcttga agggactgga caaacttaca gtcatgctga acgcattcgc
    10981 cagttggtgg cagagattga gctggctgac aaggttggtt tggatgtata tgggattggt
    11041 gagcaccatc gggcggattt tgcagtatca gccccagaga ttgttctggc cgctggggca
    11101 gtcaatacca agaaaatccg tttgaccagt gcagtcagca ttctgtcaag catggacccg
    11161 attcgtttgt tccaacagta tgccactatc gatgctttgt caaatggacg agcggagatt
    11221 atggctggaa ggggctcttt cacggaatct ttccctctgt ttggttatga cttgaaagac
    11281 tacgactctc tttttgatga gaaattagac ttgcttcagt tagtcaatga aaagaccaag
    11341 ttagactggc aaggtcgatt gactcaaacg attgctggta aagaagttta tcctcgtcca
    11401 gttcaggaca aattgccctt gtggatagct acaggtggtc atgtcgaatc aacagtgaag
    11461 attgctcagg ctggtctacc gattgtatat gctattattg gtggtaatcc acgttatttt
    11521 aaaaagttga ttcaggctta tcgtgagatt ggaagcgaag cgggtcatgc tgacaaagac
    11581 ttgaaagtgg gagcccattc ttgggggtgg attgcggaag atggtgagca ggcggtgaaa
    11641 gattatttcc atccgactaa gcaagtggtg gatgctattt ccaaagaccg tccgcactgg
    11701 caggaattgc gttatgagca atatttggag caggtagggc caaatggcgc catgtttgtt
    11761 ggcaatccag atcaggtggc cgaaaaattg attcgcatga tagaggattt aggattggac
    11821 cgcttcatgc ttcatctacc gcttggttct atgcctcatg accaggttct gagagctatt
    11881 gaactcttcg gaacacaagt tgcacccaaa gtacgagctt attttgctat gaaagaggct
    11941 taataaaaaa tcctaatcaa gtttattagg acaacaaata ttgtacgaaa attaatcccc
    12001 cgagctataa tgcttggggg atttttctat aggagggcta gtttagttct cttttttatt
    12061 ttttagtaag aaaccgagac caaggagcgc aaggaggctg attcctgcaa ataggagttt
    12121 gttttcgttt ttgattcctg tatttggaag ttcagcttgt ttatctgggg ttacaggtat
    12181 atttttcttg ctagagtttt ctaccgattc gttttgaacc gcttctttta cagatgatgc
    12241 ttgtgcttct tgtgctggtt gttcagcttg tgaatcagct gtagcttggc taggtttatt
    12301 ttccgcatca gctactttgg catctggttt agtagaatca ggtctagctt ctggagctgg
    12361 gtcttgatgt tcaggcttgg aaggggttga gtctggtttc tcttctgcag ttgactcatg
    12421 gctagttcca ttttgagaga ctcgaagaac agtagctgta agggcattca atttcaagcc
    12481 tttttcagtc cattcaagtc ctttcgggtt ggcaattccg actgatcctg cttggttttc
    12541 atctgccaaa acttccgcat ttcttagatg tgcaaaggca gttcccaaat taaattcgcg
    12601 agctttttca tccgcattga caaagactgc gtagatatcg ccgtttggag cagtgatttg
    12661 gtagccaatc actacatcct ctttttccac accattttgg cctgggacag tgatgaggtg
    12721 gacacggtct ttgatatctt ggagactctt aagtcggaag gcatctgtag attgacgaag
    12781 ggcaatcaaa cctttcatat agtcacggct cttgacattt tcaggataag ctttaccatc
    12841 tgtagcctta gtccagtcaa acttgttgac tgcgtcgcta gaatcgtaag aatcatggat
    12901 gaagtaagga tagtcaaatg ggttgccgtc cttatcacgc aacaagtgag atttgtttgg
    12961 aaccttatcc tctgctactg gagtcttgta ggctgggtca cggaattgtt tagtacgtcc
    13021 atattcctga ccggagtgga taaatggagt tccttgagct gtcaagacca tgagatttcc
    13081 aagtcgtaaa cgacggtgga tttcagcata gttctcagcc ttgcttgggt cttttttgat
    13141 agactgggca atgatgtcaa agagggtcaa gttatcatgg gctgcgatgt attggatgac
    13201 atctccaggg ctgtcagctt caaagttagt tggctgagca atgagatttt taaagatagt
    13261 gttaacatca cgcttgccac ctgtgataaa ggcaggttga ccttcgtttg gataaccaga
    13321 tttgaggttg ttacggatgt catctgaaaa gacagcgaca gtatcggtat gtttcatcca
    13381 atcttggtca gcagctttag taggcatgtt ttcatcaccg gcataggttc tccaaccttc
    13441 gcctaacatg attagatttg gattgagggc gcgtgcagcc ttgtaagctt cttcgataga
    13501 agcggcgtca tggtctccca tcatatcgaa acggaatcca tccactttgt aggtatcaac
    13561 taggtatttg atagagtcaa ctaggaggcg tttggtcata tggtgggttg tccccaagcg
    13621 tccaccacca aagctagttc gaggtgtgcc atcggcatcc ataaagtggt agtagtttgg
    13681 ctctaggtct tcaaagatat cgactttggc tgtgtggtta taaacgacat ctaggatagc
    13741 tcccatacca cgtttgtgga tttcgttgat gaggttttta aattctgcga ttcgtttttc
    13801 tggattctta ggatcgcttg agtacatacc agtcaaggag aagtagtttt gagggtcata
    13861 tccccagttg tagttgctgt tgcttgaagc atagtcagac aagcgttcat ggtttttcaa
    13921 ttcattgaca aagtagtaag acaagactgg aaggagctgg atatgggtta cacccaagtc
    13981 tttgagatag tctagttttt caatgaaggc ttcaaaagtc ccaaatggtt tggtcaagtc
    14041 ttttgcaatg gcaggatctg aagtgaagtc acgcacatga gcttcgtaga taacggcgtc
    14101 ttcacgagtc ttgaaattgt gaatcttacc ataagtcaag tcttgaggtc cgagtttagc
    14161 tggatctaca aaggcggctt tagccacttt atgggcatcg tcaatcttgg catcgtcgct
    14221 attccaagca gcaagagatt tagcgtaagg atcgagtgca agaacagttt taccttgacg
    14281 ctcgatttgg tattgataat agtagccagt gaaatctgtg attccgagtt tgtttgtgct
    14341 gtctagagtt tgtttccaag ttcctctttc ccctttttcg agagcgacag ttccaactac
    14401 tttgtcaggg tcattcttgt cgtagacaac aacagaaacc ttatcagcac ttggtgacca
    14461 aagggtcaaa tcaacttgtt ttccttcttc ttttaggtca gctcccagtt tgccatcata
    14521 gctgtatgtc tcatctttca ggcgccagct tgttttggta gtgaattggt cggaattgta
    14581 gctaacagta taaggatgtt ttgtgtcaga gaaatctccg ctgtaggtca ctttcttacc
    14641 agcttcatcg attgcaacat cggtaatagt taccttgttt cctaggtgat tagtgatgtt
    14701 ggagtgtttg aggatatctt cttttttagc accgacaagt gttgaaaagc tactttcaat
    14761 gctagaagtg cctacgtgtt gggctcctgt catacggata tcatggacat agtatggatt
    14821 tgtgtaaatc gattcatcat cgtcttttag gaaaatttgg ctatgatttt tcaaatctgt
    14881 gaacttataa ttttctttac ggattttcac gtcgtctcct tgtttgctct catctagtaa
    14941 taaaaatcca aattctcttg cggcttcatt aagaggaatg tcgatatagc ggccatattt
    15001 gcctgtagcc gtaaagtctg ttccgtcagg ccattgagcg ctacttggat tttttacatc
    15061 tccccagtac cagagagatt tcttgtcata gttgccatct gtgcggtagt agttgacgcg
    15121 aacagttcct gcaggctgtg gctcgtaaga gaaaaccttg taatcttggt ctaaccaagc
    15181 ttcgttcatt tttggaacta gtttttctac agatttatcg ccggttagat tttttccagc
    15241 tgtattgttg atgaggaagc taattttctt ggcttgttct ccctttaatt tgacatctag
    15301 gtaatagccg tagtcatctt tcttggcatc cttgaaggac aaagctccgt ttggccagtt
    15361 ttcagatggt ttttcaacat cgtcccaagt ccatagtcct tgagcatcct tgttttcttc
    15421 aggaagtttt ttgacatgga tacggaagta gttatcttcg atgggctctt ctgccttagt
    15481 ttcagttgtt actggactag tagaagtagt tggctctgtt gcaggttttt cttctgtttt
    15541 ctctagggaa gcgtttgcat ttctaggtga agaaatatca ctttcgttct taccagagcc
    15601 agctgctgta tcagtaagag gttgggcaag ggtggttgta tttctgttgt tgggttcgtt
    15661 agtggttggt gtactttcgt cggatgagat agttggtgta gccatggcaa gcaagacaag
    15721 gcttgcgccg ataaggacag aaccagttcc atttttgagg gaacggatgc tgtagaccat
    15781 ttttttctca gtgtgagatg gtgtttttct cataatgatt ctccaatcaa taaatttcta
    15841 tatcagtata gcatgtagta aaacaatatg caagcgtttt ctttgatttg tgaaaaaaat
    15901 tcctactttt gagaagtttt ctgtactatt ttatatatca gtataaatga cggagataaa
    15961 aatgtcacat aagccaagcc gtattattag gaagatgatg tgttcccagc tatttttatt
    16021 cagaaattga tctctaacat taccaataaa aatgaaaaat atcttgacaa gcaagggaaa
    16081 attttgttac aataatagac ggtacttttt acttttggtc tctcaagagt gtacagggac
    16141 gtgctgacaa atgttgcaaa agtacacaca gatgatatct gtcaccaagt gtatcatcac
    16201 caaaaataaa aaaacacagg agaatgtaga tgcctacaat taaccaattg gttcgcaaac
    16261 cgcgtaaatc aaaagtagaa aaatctaaat caccagcttt gaacgttggt tacaatagtc
    16321 ataaaaaagt tcaaacaaac gtttcttcac cacaaaaacg tggtgttgca actcgtgttg
    16381 gaacaatgac acctaaaaaa cctaactcag cccttcgtaa attcgctcgt gtacgtttga
    16441 gcaaccttat cgaagttact gcctacatcc caggtatcgg acacaacttg caagagcaca
    16501 gcgtggtgct tcttcgcggt ggacgtgtaa aagaccttcc aggggtacgt taccatatcg
    16561 tccgtggtgc acttgatact gcaggtgtta acgatcgtaa acaaggccgt tctaaatacg
    16621 gtactaaacg tccaaaagca taaggaaagg ggataaagag aaatgagtcg taaaaataga
    16681 gctccaaaac gtgacgtatt gccagatccg ctatacaatt cacaactagt tactcgtctt
    16741 atcaaccgcg ttatgcttga tggtaaacgt ggtactgctg cttcaatcgt ttacggtgct
    16801 tttgagcaaa tcaaagaagc tactggcaac gatgcacttg aagtatttga aacagctatg
    16861 gaaaacatca tgcctgtact tgaagtacgt gcacgtcgtg ttggtggttc taactaccaa
    16921 gtcccagttg aagttcgtcc agaacgtcgt acaacacttg gacttcgttg gttggtaaca
    16981 atcgctcgtc ttcgtggtga acacacaatg caagaccgtc ttgcaaaaga aatcttggat
    17041 gctgctaaca acactggtgc agcagttaag aaacgtgaag atactcaccg tatggctgaa
    17101 gctaaccgtg cattcgcaca cttccgttgg taagatagga tgcgaaagcg ttaagaaagt
    17161 cccagagaaa atagggaatc gaagcaggtt gcggttgcaa ccaatgagat tcatcttttt
    17221 ctccagactt ttagcttgag ctcaactaaa tcatgatgct aggcacggta aggatgcaag
    17281 gtgaaaatag gaaactgacg cagtattcaa cgaatacaag gagttttatc tttttcacgc
    17341 agcatcccgt tccagctcac atcggctaac taactttagc ctgggttcaa attagctaaa
    17401 tcgattagta ttagctataa ctcagcttac catctcgtaa gttgaaacca acaatagcat
    17461 gaaaacattg agaacgggta ggtcctgcct atccgttttt attaaaatcg tgttataata
    17521 gaatagaaat caaaaataaa taggagaaac aaacctcatg gcacgcgaat tttcacttga
    17581 aaaaactcgt aatatcggta tcatggctca cgtcgatgcc ggtaaaacaa caactactga
    17641 gcgtattctt tactacactg gtaaaatcca caaaatcggt gaaactcacg aaggtgcgtc
    17701 acaaatggac tggatggagc aagagcaaga acgtggtatc acgatcacat ctgctgcgac
    17761 aacagctcaa tggaacaacc accgcgtaaa catcatcgac acaccaggac acgtggactt
    17821 cacaatcgaa gtacaacgtt ctcttcgtgt attggatggt gcggttaccg ttcttgactc
    17881 acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaactg agtacggagt
    17941 tccacgtatc gtatttgcca acaaaatgga caaaatcggt gctgacttcc tttactctgt
    18001 aagcacactt cacgatcgcc ttcaagcaaa tgcacaccca atccaattgc caatcggttc
    18061 tgaagatgac ttccgtggta tcattgactt gatcaagatg aaagctgaaa tctatactaa
    18121 cgaccttggt acggatatcc ttgaagaaga catcccagct gaataccttg accaagctca
    18181 agaataccgt gaaaaattga ttgaagcagt tgctgaaact gacgaagaat tgatgatgaa
    18241 atacctcgaa ggtgaagaaa tcactaacga agaattgaaa gctggtatcc gtaaagcgac
    18301 tatcaacgtt gaattcttcc cagtattgtg tggttcagcc ttcaaaaaca aaggtgttca
    18361 attgatgctt gatgcggtta tcgactacct tccaagtcca cttgacatcc cagcaatcaa
    18421 aggtattaac ccagatacag acgctgaaga aattcgtcca gcatctgacg aagagccatt
    18481 tgcagctctt gccttcaaga tcatgactga cccattcgta ggtcgtttga cattcttccg
    18541 tgtttactca ggtgttcttc aatcaggttc atacgtattg aatacttcta aaggtaaacg
    18601 tgaacgtatc ggacgtatcc ttcaaatgca cgctaacagc cgtcaagaaa tcgacactgt
    18661 ttactcaggt gatatcgctg ctgccgttgg tttgaaagat actacaactg gtgactcatt
    18721 gacagatgaa aaagctaaaa tcatccttga gtcaatcaac gttccagaac cagttatcca
    18781 attgatggtt gagccaaaat ctaaagctga ccaagacaag atgggtatcg cccttcaaaa
    18841 attggctgaa gaagatccaa cattccgcgt tgaaacaaac gttgaaactg gtgaaacagt
    18901 tatctcaggt atgggtgaac ttcaccttga cgtccttgtt gatcgtatgc gtcgtgagtt
    18961 caaagttgaa gcgaacgtag gtgctcctca agtatcttac cgtgaaacat tccgcgcttc
    19021 tactcaagca cgtggattct tcaaacgtca gtctggtggt aaaggtcaat tcggtgatgt
    19081 atggattgaa tttactccaa acgaagaagg taaaggattc gaattcgaaa acgcaatcgt
    19141 cggtggtgtg gttcctcgtg aatttatccc agcggttgaa aaaggtttgg tagaatctat
    19201 ggctaacggt gttcttgcag gttacccaat ggttgacgtt aaagctaagc tttatgatgg
    19261 ttcatatcac gatgtcgact catctgaaac tgccttcaag attgcggctt cactttccct
    19321 taaagaagct gctaaatcag cacaaccagc tatccttgaa ccaatgatgc ttgtaacaat
    19381 cactgttcca gaagaaaacc ttggtgatgt tatgggtcac gtaactgctc gtcgtggacg
    19441 tgtagatggt atggaagcac acggtaacag ccaaatcgtt cgtgcttacg ttccacttgc
    19501 tgaaatgttc ggttacgcaa cagttcttcg ttctgcatct caaggacgtg gtacattcat
    19561 gatggtattt gaccactacg aagatgtacc taagtcagta caagaagaaa ttattaagaa
    19621 aaataaaggt gaagactaat ccgtcctcac tctagaagga agtcacttag tggcttcctt
    19681 ttgtctttag aaaatacctc taaatatggt aaaatagtag aagaataatg tgaggaaaat
    19741 gaatgtcaaa tagttttgaa attttgatga atcaattggg gatgcctgct gaaatgagac
    19801 aggctcctgc tttagcacag gccaatattg agcgagttgt ggttcataaa attagtaagg
    19861 tatgggagtt tcatttcgta ttttctaata ttttaccgat tgaaatcttt ttagaattaa
    19921 agaaaggttt gagcgaagaa ttttctaaaa caggcaataa agctgttttt gaaattaagg
    19981 ctcggtctca agaattttca aatcagctct tgcagtccta ctatagggag gctttctctg
    20041 aaggtccatg tgctagtcaa ggttttaagt ccctttatca aaatttgcaa gttcgtgctg
    20101 agggtaatca gctatttatt gaaggatctg aagcgattga taaggaacat tttaagaaga
    20161 atcatcttcc taatttagcc aaacaacttg aaaagtttgg ttttccaact tttaactgtc
    20221 aagtcgagaa gaatgatgtc ctgacccaag agcaggaaga ggcctttcat gctgaaaatg
    20281 agcagattgt tcaagctgcc aatgaggaag cgctccgtgc tatggaacaa ctggagcaga
    20341 tggcacctcc tccagcggaa gagaaaccag tctttgattt tcaagcgaaa aaagctgcag
    20401 ctaaacccaa gctggataag gcggagatta ctcctatgat cgaagtgacg acagaggaaa
    20461 atcgtctggt atttgaaggg gttgtttttg atgtggagca aaaagtgact agaacaggtc
    20521 gtgttttaat caactttaaa atgacggact atacttcaag tttttctatg caaaagtggg
    20581 ttaaaaacga ggaagaggcc cagaagtttg acctcatcaa gaagaattct tggctccgag
    20641 ttcgagggaa tgtggagatg aataacttca cacgcgattt gactatgaac gtacaggatc
    20701 tgcaggaagt tgttcactat gagcggaagg atttgatgcc agaaggtgag cgtcgggttg
    20761 agtttcatgc tcatactaac atgtcgacta tggatgcttt gccagaggtc gaagagattg
    20821 ttgcaacagc tgctaagtgg ggacacaagg cggttgctat cacagaccat gggaatgtcc
    20881 agtcctttcc acatggctat aaggcggcta agaaagcggg aatccagctg atctatggga
    20941 tggaagccaa tatcgtggag gaccgtgtcc ctatcgtcta taacgaagtg gagatggact
    21001 tatcagaagt aacctacgtg gtctttgacg tggaaacgac gggactttca gctatctata
    21061 atgacttgat tcaggttgcg gcctctaaga tgtacaaggg gaatgttatt gctgaatttg
    21121 atgaatttat caatcctggg catcccttgt cagcttttac tacagagtta actggaatta
    21181 cagatgatca tgtcaaaaat gccaaaccac tagaacaagt tttgcaagaa ttccaagaat
    21241 tttgcaagga tacggtccta gttgcccaca atgctacctt tgacgttggc tttatgaatg
    21301 ctaattatga gcgtcatgat cttccaaaga ttagtcagcc agttattgat acgctggagt
    21361 ttgctagaaa cctctatcct gagtataaac gccatggttt ggggcctttg accaagcgtt
    21421 ttggtgtggc cttggaacat caccacatgg ccaactacga tgcggaagcg actggtcgtc
    21481 tgcttttcat ctttatcaaa gaggtagcag aaaaacatgg tgtgaccgat ttagctagac
    21541 tcaacattga tctaatcagt ccagattctt acaaaaaagc tcggatcaag catgcgacca
    21601 tctatgtcaa gaatcaggta ggtctaaaaa atatctttaa gctggtttcc ttgtctaata
    21661 ccaagtattt tgaaggagtg ccacggattc cgagaacggt tctagatgcc catcgagagg
    21721 gcttgatttt aggttcagcc tgttcagagg gtgaagtttt tgacgtggtc gtttctcaag
    21781 gtgtggatgc ggcggttgag gtggccaagt attatgactt tatcgaggtc atgccaccgg
    21841 ctatctatgc gcccttgatt gctaaagagc aggtcaagga tatggaggaa ctccagacca
    21901 ttatcaagag tttgatagag gttggagacc gccttggcaa gcctgttctg gctacgggaa
    21961 atgttcacta tatcgaaccg gaagaagaga tttatcgtga aattatcgtc cgtagtttgg
    22021 gacagggtgc gatgattaac cgaactatcg gtcatggtga acatgcccaa ccagcgccac
    22081 ttccaaaggc tcattttcga acgactaatg agatgttgga tgaatttgcc tttttgggag
    22141 aggaactggc tcgtaaactg gttattgaaa acactaatgc cttggcagaa atatttgaac
    22201 ccgttgaagt cgttaagggt gacttgtata cgcctttcat caacaaggct gaagaaacag
    22261 ttgctgagtt gacctataag aaagcttttg agatttatgg aaatccgctg ccagatattg
    22321 ttgatttgcg gattgaaaaa gaattaacat ccatactggg gaatggattt gctgtgattt
    22381 atctggcatc gcagatgctg gtgcaacgtt ctaatgaacg gggttatttg gttggttctc
    22441 gtgggtctgt cggatctagt ttcgttgcga ccatgattgg gattacggag gtcaatcctc
    22501 tctctcctca ctatgtctgt ggtcagtgtc agtacagtga gtttatcaca gatggttcgt
    22561 acggttcagg atttgatatg ccccataagg actgtccaaa ctgtggtcac aaactcagta
    22621 aaaacggaca ggatattccg tttgagacct tccttggttt tgatggggat aaggttcctg
    22681 atattgactt gaacttctcg ggagaagatc agcctagcgc ccacttggat gtgcgtgata
    22741 tctttggtga agaatatgcc ttccgtgcgg gaacggttgg tacggtagct gccaagactg
    22801 cctatggatt tgtcaagggt tacgagcgag attatggcaa gttttatcgt gatgcagaag
    22861 tagaacgcct cgctcaagga gcggcgggtg tcaagcggac aacaggccaa cacccggggg
    22921 gaatcgttgt tattccgaac tacatggatg tctacgattt tacgcctgtc cagtatccag
    22981 cagatgatgt cacggctgaa tggcagacca ctcactttaa cttccacgat atcgatgaga
    23041 acgtcctcaa actcgatgta ctgggacatg atgatccgac catgattcga aaacttcagg
    23101 atttgtctgg tattgaccct aataaaattc ctatggatga cgaaggcgtg atggcactct
    23161 tttctgggac tgatgtgcta ggggtaacac ctgaacaaat tggaacgcct acgggtatgt
    23221 tggggattcc agagtttgga acaaatttcg tacgtggaat ggtagacgaa acccatccga
    23281 caacctttgc ggaattgctt cagctgtctg gtctgtccca cggtactgac gtttggttgg
    23341 ggaatgctca ggatctgatt aagcaaggaa tagcggacct atcgactgtt atcggttgtc
    23401 gggacgacat catggtttac ctcatgcatg cgggtctgga acctaagatg gcctttacca
    23461 ttatggaacg ggtacgtaag ggtttgtggc taaagatttc agaagaggag agaaatgact
    23521 atatcgaagc catgaaggcc aataaggtgc cagagtggta tatcgaatcc tgtgggaaaa
    23581 ttaagtacat gttccctaag gcccatgcgg cagcctacgt tatgatggcc ttgcgtgtag
    23641 cttacttcaa ggttcaccat cctatttatt attactgtgc ttacttctcc attcgtgcta
    23701 aggcttttga tatcaagacc atgggtgcgg gcttggaggt catcaagcgc agaatggaag
    23761 aaatctctga aaaacggaag aacaatgaag cctctaatgt ggaaatcgat ctctatacaa
    23821 ctcttgagat tgtcaatgag atgtgggaac gaggtttcaa gtttggcaaa ttagatctct
    23881 accgtagtca ggcgacagag ttcctcatcg acggggatac ccttatccca ccatttgtag
    23941 caatggatgg tctgggagag aacgttgcca agcaactggt gcgggcgcgt gaagagggag
    24001 aattcctctc taaaacagaa ctacgcaagc gtggtggact ctcatcaacc ttggttgaaa
    24061 agatggatga gatgggtatt cttggaaata tgccagagga taaccagttg agtttgtttg
    24121 atgagttgtt ttaaaaaatt gcttaataat ctattaaaag aggctaacgt atatccaata
    24181 gatttacatt agctttcttt tttgttaaaa tagtctatgg aaagagggtg agagtatgtc
    24241 aaagatgagt ataagcatcc gtctggatag tgaggttaag gagcaggccc aacaggtgtt
    24301 tagtaatctg ggaatggata tgacaacagc tattaatatt ttccttcgtc aggcaattca
    24361 atatcaggga ttaccttttg atgttagact agacgaaaat cggaagttgc tccaagcgtt
    24421 aacggattta gaccaaaatc gtaatatgag ccagtctttt gaatcagtct cagatttgat
    24481 ggaggactta cgtgcttaag attcgttatc ataaacagtt taaaaaagat tttaagttgg
    24541 ctatgaagcg tggtttgaag gcagaattat tagaagaagt tttgaatttt ctggttcaag
    24601 aaaaagaaca tcctgccaga tatcgtgatc attcattgac ggcatccaag cattttcaag
    24661 gagttcgtga atgccatacc cagccagatt ggcttttggt ttataaagta gacaagtcgg
    24721 aattgatttt aaatttgctg aggacaggca gtcacagtga tttattttaa tctattttaa
    24781 gggggttctc atgaaactaa gaatatttgc ggaagataag ccggctaaga aggtatttga
    24841 atatcaatta gaacttgctg atcgtacaat tcttctatcg acagcactct tgtcaggtgc
    24901 tattgcttta gcaggaatct tttctgcttt gagagaaaaa taaaaataga aaagagaaaa
    24961 cagaatggtt ttaccaaatt ttaaagaaaa tctagaaaaa tatgcgaaat tgttggttgc
    25021 gaacggaatt aacgtgcaac ctggtcacac tttggctctt tctattgatg tggagcaacg
    25081 tgaattggca catctaatcg tgaaagaagc ttatgccttg ggtgcgcatg aggtcatcgt
    25141 tcagtggaca gatgatgtga ttaaccgtga gaaattcctc catgccccga tggagcgttt
    25201 ggacaatgtg ccagaataca agattgctga gatgaactat ctcttggaga acaaggctag
    25261 ccgtcttgga gttcgttcat ctgatccagg tgccttgaac ggagtggacg ctgacaagct
    25321 ttcagcttct gctaaagcta tgggacttgc catgaagcct atgcgtatcg caactcaatc
    25381 taacaaggtt agctggactg tagcagctgc agcaggactt gagtgggcta agaaagtctt
    25441 cccaaatgct gcgagcgacg aagaagcagt tgatttcctt tgggaccaaa ttttcaaaac
    25501 ttgccgtgtc tacgaagcag atcctgttaa ggcttgggag gaacatgcag ccattctcaa
    25561 gagcaaggcc gatatgctta ataaggagca attttcagcc cttcactaca cagcgccagg
    25621 aacagattta acacttggtt tgccaaagaa ccacgtttgg gaatcagctg gtgctgtcaa
    25681 tgcacagggc gaagaattct tgccaaatat gccaacagaa gaggtcttca cagcgcctga
    25741 cttccgtcgt gcagatggtt atgtcacttc tacaaaaccg cttagctaca acggaaatat
    25801 cattgaaggc attaaggtga cctttaagga tggacaaatc gtagatatca ctgctgagaa
    25861 gggtgatcag gttatgaaag accttgtctt tgaaaatgcg ggtgcgcgtg ccttgggtga
    25921 atgtgccttg gtaccagatc caagtccaat ttctcagtca ggcattacct tctttaacac
    25981 ccttttcgat gaaaatgcgt caaaccactt ggctatcggt gcagcctatg cgactagcgt
    26041 tgttgatgga gcggagatga gcgaagagga gcttgaagct gcagggctta accgttcaga
    26101 tgttcacgta gactttatga ttggttctaa ccaaatggat atcgatggta ttcgtgagga
    26161 tggaacgcgg gtacctcttt tccgtaatgg gaattgggca aattaaggag ataatatgtt
    26221 aggaagtatg ttcgttggtc tcctagtggg atttttagca ggtgctatga ccaatcgtgg
    26281 agagcgaatg ggatgttttg gaaaaatgtt tctcggttgg atcggagcct ttctaggtca
    26341 cttgctcttt ggaacttggg ggccagtttt atcaggaaca gctattatcc cagcgatttt
    26401 aggagccatg attgttttag ctattttttg gagacgagga agttaatttc ctaaatctga
    26461 tactcaatga aaatcaaaga gcaaactagg aaactagccg caggttgctc aaagcact
//
LOCUS       DAVBDG010000026        26286 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBDG010000026 DAVBDG010000000
VERSION     DAVBDG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.026"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..919
                     /gene="lytB"
                     /locus_tag="VQ526_001064"
     CDS             <1..919
                     /gene="lytB"
                     /locus_tag="VQ526_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET2554798.1"
                     /translation="KSGGYMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTAN
                     VYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIP
                     YYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTE
                     ATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSK
                     IAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGM
                     NVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(980..2635)
                     /gene="pavA"
                     /locus_tag="VQ526_001065"
     CDS             complement(980..2635)
                     /gene="pavA"
                     /locus_tag="VQ526_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2554799.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2740..3237
                     /gene="ybeY"
                     /locus_tag="VQ526_001066"
     CDS             2740..3237
                     /gene="ybeY"
                     /locus_tag="VQ526_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2554800.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3218..3613
                     /locus_tag="VQ526_001067"
     CDS             3218..3613
                     /locus_tag="VQ526_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2554801.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3630..4529
                     /gene="era"
                     /locus_tag="VQ526_001068"
     CDS             3630..4529
                     /gene="era"
                     /locus_tag="VQ526_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2554802.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4578..5402
                     /gene="mutM"
                     /locus_tag="VQ526_001069"
     CDS             4578..5402
                     /gene="mutM"
                     /locus_tag="VQ526_001069"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2554803.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYHKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFCLTDKVLISHLRMEGKYFYYPDQVPERKHAHVFFRFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5402..6007
                     /locus_tag="VQ526_001070"
     CDS             5402..6007
                     /locus_tag="VQ526_001070"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2554804.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            5994..7193
                     /locus_tag="VQ526_001071"
     CDS             5994..7193
                     /locus_tag="VQ526_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HET2554805.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7193..7342
                     /gene="rpmG"
                     /locus_tag="VQ526_001072"
     CDS             7193..7342
                     /gene="rpmG"
                     /locus_tag="VQ526_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2554806.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7382..7615
                     /gene="secG"
                     /locus_tag="VQ526_001073"
     CDS             7382..7615
                     /gene="secG"
                     /locus_tag="VQ526_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2554807.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7717..10071
                     /gene="rnr"
                     /locus_tag="VQ526_001074"
     CDS             7717..10071
                     /gene="rnr"
                     /locus_tag="VQ526_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2554808.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLKLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     AGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKKYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGESYADVLSMMLLRSMQQARYSEHNYGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10034..10501
                     /gene="smpB"
                     /locus_tag="VQ526_001075"
     CDS             10034..10501
                     /gene="smpB"
                     /locus_tag="VQ526_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2554809.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKRQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10517..11377
                     /gene="tehB"
                     /locus_tag="VQ526_001076"
     CDS             10517..11377
                     /gene="tehB"
                     /locus_tag="VQ526_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2554810.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMETVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            11450..12403
                     /gene="coiA"
                     /locus_tag="VQ526_001077"
     CDS             11450..12403
                     /gene="coiA"
                     /locus_tag="VQ526_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2554811.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            12422..14224
                     /gene="pepF"
                     /locus_tag="VQ526_001078"
     CDS             12422..14224
                     /gene="pepF"
                     /locus_tag="VQ526_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2554812.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            14226..14939
                     /locus_tag="VQ526_001079"
     CDS             14226..14939
                     /locus_tag="VQ526_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2554813.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            15006..15947
                     /gene="prsA"
                     /locus_tag="VQ526_001080"
     CDS             15006..15947
                     /gene="prsA"
                     /locus_tag="VQ526_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2554814.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDIITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(16064..16405)
                     /locus_tag="VQ526_001081"
                     /pseudo
     CDS             complement(16064..16405)
                     /locus_tag="VQ526_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            16818..16913
                     /locus_tag="VQ526_001082"
                     /pseudo
     CDS             16818..16913
                     /locus_tag="VQ526_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17166..17786)
                     /locus_tag="VQ526_001083"
     CDS             complement(17166..17786)
                     /locus_tag="VQ526_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2554815.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(17786..18268)
                     /locus_tag="VQ526_001084"
     CDS             complement(17786..18268)
                     /locus_tag="VQ526_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2554816.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(18268..19110)
                     /locus_tag="VQ526_001085"
     CDS             complement(18268..19110)
                     /locus_tag="VQ526_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2554817.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(19205..20005)
                     /locus_tag="VQ526_001086"
     CDS             complement(19205..20005)
                     /locus_tag="VQ526_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2554818.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            20149..21528
                     /gene="glmU"
                     /locus_tag="VQ526_001087"
     CDS             20149..21528
                     /gene="glmU"
                     /locus_tag="VQ526_001087"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2554819.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILESLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNKRLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            21538..22083
                     /locus_tag="VQ526_001088"
     CDS             21538..22083
                     /locus_tag="VQ526_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2554820.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            22095..22409
                     /locus_tag="VQ526_001089"
     CDS             22095..22409
                     /locus_tag="VQ526_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2554821.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            22426..23118
                     /locus_tag="VQ526_001090"
     CDS             22426..23118
                     /locus_tag="VQ526_001090"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2554822.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIVSHEVV
                     LVESGIGKVMSAMSVAILAVHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            23195..23686
                     /gene="rocS"
                     /locus_tag="VQ526_001091"
     CDS             23195..23686
                     /gene="rocS"
                     /locus_tag="VQ526_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2554823.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            23760..24350
                     /locus_tag="VQ526_001092"
     CDS             23760..24350
                     /locus_tag="VQ526_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2554824.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            24359..26122
                     /locus_tag="VQ526_001093"
     CDS             24359..26122
                     /locus_tag="VQ526_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2554825.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         7982 a         4784 c         5774 g         7746 t
ORIGIN      
        1 caaatctggt ggttacatgg cgaaaaatga gacagtagat ggttatcagc ttggaagcga
       61 tggtaaatgg cttggaggaa aagctacaaa tgaaaatgct gcttactatc aagtagtgcc
      121 tgttacagcc aatgtttatg attcagatgg tgaaaagctt tcctatatat cgcaaggtag
      181 tgtcgtatgg ctagataagg atagaaaaag tgatgacaag cgcttggcta ttactatttc
      241 tggtttgtca ggctatatga aaacagaaga tttacaagcg ctagatgcta gtaaggactt
      301 tatcccttat tatgagagtg atggccaccg tttttatcac tatgtggctc agaatgctag
      361 tatcccagta gcttctcatc tttctgatat ggaagtaggc aagaaatatt attcggcaga
      421 tggcctgcat tttgatggtt ttaagcttga gaatcccttc cttttcaaag atttaacaga
      481 ggctacaaac tacagtgctg aagaattgga taaggtattt agtttgctaa acattaacaa
      541 tagccttttg gagaacaagg gcgctacttt taaggaagcc gaagaacatt accatatcaa
      601 tgctctttat ctccttgccc atagtgccct agaaagtaac tggggaagaa gtaaaattgc
      661 caaagataag aataatttct ttggcattac agcctatgat acgacccctt acctttctgc
      721 taagacattt gatgatgtgg ataagggaat tttaggtgca accaagtgga ttaaggaaaa
      781 ttatatcgat aggggaagaa ctttccttgg aaacaaggct tctggtatga atgtggaata
      841 tgcttcagac ccttattggg gcgaaaaaat tgctagtgtg atgatgaaaa tcaatgagaa
      901 gctaggtggc aaagattagt actataagtg aatatgattt gagtgaatag taagttaaaa
      961 atcctgattt caagtaaaat caggattttt tcatggatgc aatttttttg gagtctggtg
     1021 tgacgcggag ggtcttttgt cctgtgtaag tgacaaagcc gggttttcca ccagttggtt
     1081 tattgagttt tttgacttca atcatatcta cctgcaccag attcgacagg cgcccttgag
     1141 agaagtaggc agctaactct gctgcgtctg tcttgactgc atcagatggg tcaagatttc
     1201 ctgagatgac aacatggctt ccaggaatgt ccttagcatg gaaccaaagt tcctccttgc
     1261 gggccatttt aaaggtcaat tcctcatttt gaagattgtt tcgtccgaca tagatgatgg
     1321 ttttgccatc gcttgctaga tattgttcta gttttttgcg tttctggatt ttctcccgtt
     1381 gtcttctgcg gataaaacct gtttgaatca attcttcacg gatttcagcg atttcttcca
     1441 gtccagcttg gttgaggacg gtttctacac tttccagata gagaatagtg gctttggttt
     1501 cttcaatcaa atcagtcaag tatttgacag cttctttgag tttctgatac cgtttaaaat
     1561 agcgttgggc attctggttg ggagtcagag ccttatcaag cgcaatcatg ataggttggt
     1621 tggtatagta gttgtctagg ataacctggt cttggtcgtt aggcacttgg tggaggaagg
     1681 ttgtcagcaa ttctcctttt tgacgaaatt cttcagcgtt gtctgtcgcc agtaactctt
     1741 tttcctgttt tttgagtttg tgtcggtttt tctgaagttc attttcaaca cgacgaatca
     1801 gttcactggc ctgctgtttg acgcggtcgc gctcagcctt atccttatag taggtgtcca
     1861 acaaatcaga aagatttgca agaggctctc ccacctgatt tgcaaaagga actggactga
     1921 aggaagtctc agtcaagcat ggcttggttt cttgattgaa aaaatttcgg aaagcggaaa
     1981 gtttttcact aaccagtatc ctttccaatt catttgccgt atcgcgtccc agaccttgaa
     2041 agaggctttg aagatttttt gctgttagtt cttgggtttg caggatttca aagagctttt
     2101 catccttgat agtaaaagga ttgagagatt ttgtacttgg cggagcgata taggtcgatc
     2161 ctggaagtaa ggtgcggtag ctattttgtg aaaagccgac gtgtttgata acttcgagga
     2221 ttttatgact gcttttatcg accagtagaa tattactgtg tttccccata atttcgataa
     2281 tcaaggtagc ctggatatgg tctccaatct cgtttttatt ggaaactgta atttccacaa
     2341 tacggtcatt ttccacttgc tcaatcgact caatcagggc cccctgcaaa tactttctca
     2401 aaaccatgat aaaggtagaa ggttgggctg gattttcaaa agtcgtttgg gtcagctgaa
     2461 tgcgtccaaa aactggatgg gcagaaagga gcaggcgatg gctttggcga ttgctgcgga
     2521 tttgcaagac caactcttgt tcaaaaggct gattgatttt ctggatgcga ccattcacta
     2581 attcgcttcg caattcctca actatgtggt gtaaaaaaaa tccgtcaaat gacatcgttc
     2641 tctccttgtg attgtattcc atagtattat atcaaaaagg tagaataaaa tcatggaaat
     2701 gtggtataat aaagccaagt aaagagaaac gagaagcaca tgtatattga aatggtagat
     2761 gaaactggtc aagtttcaaa agaaatgttg caacaaaccc aagaaatttt ggaatttgca
     2821 gcccaaaaat taggaaaaga agacaaggag atggcagtca cttttgtgac caatgagcgt
     2881 agtcatgaac ttaatctgga gtaccgtgac accgaccgtc cgacagatgt catcagcctt
     2941 gagtataaac cagaattgga aattgccttt gacgaagagg atttgcttga aaatccagaa
     3001 ttggcagaga tgatgtctga gtttgatgcc tatattgggg aattgttcat ctctatcgat
     3061 aaggctcatg agcaggccga agaatatggt cacagctttg agcgtgagat gggcttcttg
     3121 gcagtacacg gctttttaca tattaacggc tatgatcact acactccgga agaagaagcg
     3181 gagatgttcg gtttacaaga agaaattttg acagcctatg gactcacaag acaataaacg
     3241 aaaatggaaa aatcgtgact tgatatccag tttagaattt gctttgacag gtatttttac
     3301 tgctatcaag gaagaacgca atatgcgaaa acacgcagtg acggctctag tggtcatcct
     3361 tgcaggtttt gtttttcagg tgtcacgaat cgaatggctc tttctcctat tgagtatttt
     3421 cttggtagta gcctttgaga ttatcaactc tgctattgaa aatgtggtgg atttggccag
     3481 tcactatcac ttttccatgc tggctaaaaa tgccaaggat atggcggctg gcgcggtatt
     3541 agtggtttct cttttcgcag ccttaacagg cgcattgatt tttctcccac gaatctggga
     3601 tttattattt taaacagtaa gaggaaatta tgacttttaa atcaggcttt gtagccattt
     3661 taggacgtcc caatgttggg aagtcaacct ttttaaatca cgttatgggg caaaagattg
     3721 ccatcatgag tgacaaggcg cagacaacgc gcaataaaat catgggaatt tacacgactg
     3781 ataaggagca aattgtcttt atcgacacac cagggattca caaacctaaa acagctctcg
     3841 gagatttcat ggttgagtct gcctacagta cccttcgcga agtggacacc gttcttttca
     3901 tggtgcctgc tgatgaagcg cgtggtaagg gggacgatat gattatcgag cgtctcaagg
     3961 ctgccaaggt tcctgtgatt ttggtggtga ataaaatcga taaggtccat ccagaccagc
     4021 tcttgtctca gattgatgac ttccgtaatc aaatggactt taaggaaatt gttccaatct
     4081 cagcccttca gggaaataac gtgtctcgtc tagtggatat tttgagtgaa aatctggatg
     4141 aaggtttcca atatttcccg tctgatcaaa tcacagacca tccagaacgt ttcttagttt
     4201 cagaaatggt tcgcgagaaa gtcttgcacc taactcgtga agagattccg cattctgtag
     4261 cagtagttgt tgactctatg aaacgagacg aagagacaga caaggttcac atccgtgcaa
     4321 ccatcatggt cgagcgcgat agccaaaaag ggattatcat cggtaaaggt ggcgctatgc
     4381 ttaagaaaat cggtagcatg gcccgtcgtg atatcgaact catgctagga gacaaggtct
     4441 tcctagaaac ctgggtcaag gtcaagaaaa actggcgcga taaaaagcta gatttggctg
     4501 actttggcta taatgaaaga gaatactaag tagaggtagg ctcatgcctg cttcttgttt
     4561 ttacagaagg aggacttatg cctgaattac ctgaggttga aaccgtttgt cgtggcttag
     4621 aaaaattgat tataggaaag aagatttcga gtatagaaat tcgctaccac aagatgatta
     4681 agacggattt ggaagagttt caaagggaat tgcctagtca gattatcgag tcaatgggac
     4741 gtcgtggaaa atatttgctt ttctgcctga cagacaaggt cttgatttcc catttgcgga
     4801 tggagggcaa gtatttttat tatccagacc aagtgcctga acgcaagcat gcccatgttt
     4861 tcttccggtt tgaagatggg ggcacgcttg tttatgagga tgtacgcaag tttggaacca
     4921 tggaactctt ggtgcctgac cttttagacg cctactttat ttctaaaaaa ttaggtcctg
     4981 aaccaagcga acaagacttt gatttacagg tctttcaagc tgcccttgcc aagtccaaaa
     5041 agcctatcaa atcccatctc ctagaccaga ccttggtagc tggacttggc aatatctatg
     5101 tggatgaggt tctctggcga gctcaggttc atccagctag accttcccag actttgacag
     5161 cagaagaagc gactgccatt catgaccaga ccattgctgt tttgggccag gctgttgaaa
     5221 aaggcggttc caccattcgg acctatacca atgcctttgg ggaagatgga agcatgcagg
     5281 attttcatca ggtctatgat aagactggtc aagaatgtgt acgctgtggt accatcattg
     5341 agaaaattca actaggcgga cgaggcaccc acttttgtcc aaactgtcaa aggagggact
     5401 gatgggaaaa atcatcggaa tcactggggg aattgcctca ggtaagtcaa ctgtgacaaa
     5461 ttttctaaga cagcaaggct ttcaagcagt ggatgccgac gcagtcgtcc accaactaca
     5521 gaaacctggt ggtcgtctgt ttgaggctct agtacagcac tttgggcaag aaatcattct
     5581 tgaaaacgga gaactcaatc gccctctcct agctagtctc atcttttcaa atcctgaaga
     5641 gcaaaaatgg tctaatcaaa ttcaagggga gattatccgt gaggaactgg ctactttgag
     5701 agaacagttg gctcagacag aagagatttt cttcatggat attcccctac tttttgagca
     5761 ggactacagc gattggtttg ctgagacttg gttggtctat gtggaccgag atgcccaagt
     5821 agaacgctta atgaaaaggg accagttgtc caaagatgaa gctgagtctc gtctggcagc
     5881 ccagtggcct ttagaaaaaa agaaagattt ggccagccag gttcttgata ataatggcaa
     5941 tcagaaccaa cttcttaatc aagtgcatat ccttcttgag ggaggtaggc aagatgacag
     6001 agattaactg gaaggataat ctgcgtattg cctggtttgg taattttctg acaggagcca
     6061 gtatttcttt ggttgtacct tttatgccca tcttcgtgga aaatctaggt gtagggagtc
     6121 agcaagtcgc tttttatgca ggcttagcaa tttctgtctc tgctatttcc gcggcgctct
     6181 tttctcctat ttggggtatt cttgctgaca aatacggccg aaaacccatg atgattcggg
     6241 caggtcttgc tatgactatc actatgggag gcttggcctt tgtcccaaat atctattggt
     6301 taatctttct tcgtttacta aacggtgtat ttgcaggttt tgttcctaat gcaacggcac
     6361 tgatagccag tcaggttcca aaggagaaat caggctctgc cttaggtact ttgtctacag
     6421 gcgtagttgc aggcactcta actggtccct ttattggtgg ctttatcgca gaattatttg
     6481 gcattcgtac agttttctta ctggttggta gttttctatt tttagctgct attttgacta
     6541 tttgctttat caaggaagat tttcaaccag tagccaagga aaaggctatt ccaacaaagg
     6601 aattatttac ctcggttaaa tatccctatc ttttgctcaa tctcttttta accagttttg
     6661 tcatccaatt ttcagctcaa tcgattggcc ctattttggc tctttatgta cgcgacttag
     6721 ggcagacaga gaatcttctt tttgtctctg gtttgattgt gtccagtatg ggcttttcca
     6781 gcatgatgag tgcaggagtc atgggcaagc taggtgacaa ggtgggcaat catcgtctct
     6841 tggttgtcgc ccagttttat tcagtcatca tctatctcct ctgtgccaat gcctctagcc
     6901 cccttcaact aggactctat cgtttcctct ttggattggg aaccggtgcc ttgattcccg
     6961 gggttaatgc cctactcagc aaaatgactc ccaaagccgg catttcgagg gtctttgcct
     7021 tcaatcaggt attcttttat ctgggaggtg ttgttggtcc catggcaggt tctgcagtag
     7081 caggtcaatt tggctaccat gctgtctttt atgcgacgag cctttgtgtt gcctttagtt
     7141 gtctctttaa cctgattcaa tttcgaacat tattaaaagt aaaggaaatc tagtgcgagt
     7201 aaaaattaat ctcaaatgct cctcttgtgg cagtatcaat tacctaacca gtaaaaattc
     7261 aaaaacccat ccagacaaga ttgaggtttt aaagtattgt cccaaggaaa gaaaagtaac
     7321 cctacatctt gaatctaagt agattttatg gtaaaataat agggatttta aggagtttga
     7381 tatgtataac ctattattaa ccattttatt agtattatct gttgtgattg tgattgcaat
     7441 tttcatgcaa ccaaccaaaa accaatccag caatgtattt gatgccagtt caggtgattt
     7501 gtttgaacgc agtaaagctc gcggttttga agctgtaatg cagcgtttga cagggatttt
     7561 agtctttttc tggctagcca ttgccttagc attgacggta ttatcaagta gataagaaaa
     7621 taatgggcag gactaggtct ttgcctcttt ttatttttaa aggatgtttg agaaggtttt
     7681 acagtaaaag aaaattaaaa aatctagaaa gaaaatatga aagatagaat aaaagaatat
     7741 ttacaagaca agggaaaggt gactgttaat gatttggctc aggctttggg aaaagacagt
     7801 tccaaggatt ttcgtgagtt gattaaaacc ttgtccttaa tggaaagaaa gcaccaaatt
     7861 cgttttgaag aagatggtag tctgaaatta gaaattaaga aaaaacatga gattaccctc
     7921 aaggggattt ttcatgccca taaaaatggc tttggctttg ttagtctgga aggcgaggag
     7981 gacgaccttt ttgtagggaa aaatgatgtc aactatgcta ttgctggtga taccgtcgag
     8041 gtagtgatta agaaagtcgc tgaccgcaat aagggaacag cagcagaagc caaaattatt
     8101 gatatcctag aacacagttt gacaacagtt gtcgggcaaa tcgttctgga tcaggaaaaa
     8161 cctaagtatg ctggctatat tcgttcaaaa aatcagaaaa tcagtcaacc gatttatgtt
     8221 aagaaaccag ccctaaaatt agaaggaaca gaagttctca aagtctttat cgataaatac
     8281 ccaagcaaga aacatgattt ctttgtcgcg agtgttctcg atgtggtggg acactcaacg
     8341 gatgtcggaa ttgatgttct tgaggttttg gaatcaatgg acattgtatc cgagtttcca
     8401 gaagctgttg ttaaggaagc agaaagtgtg cctgatgctc cgtctcaaaa ggatatggaa
     8461 ggtcgtctgg atctaagaga tgaaattacc tttaccattg acggtgcgga tgccaaggac
     8521 ttggacgatg cagtgcatat caaggctctg aaaaatggca atctggagct tggggttcac
     8581 atcgcagatg tttcttatta tgtgaccgag gggtctgccc ttgacaagga agcccttaac
     8641 cgtgcgactt ctgtttacgt gacagaccga gtggtgccaa tgcttccaga acgactatca
     8701 aatggcatct gctctctcaa tccccaagtt gaccgcctga cccagtctgc tattatggag
     8761 attgataaac atggtcgtgt ggtcaactat accattacac aaacagttat caagaccagt
     8821 tttcgtatga cctatagcga tgtcaatgat atcctagctg gcgatgaaga aaagagaaaa
     8881 aaatatcata aaattgtacc aagtatcgaa ctcatggcca agcttcatga aactttagaa
     8941 aacatgcgtg tgaaacgtgg agctctcaat tttgatacca atgaagcgaa gattttagtg
     9001 gataaacaag gtaagcctgt tgatatcgtt cttcggcagc gtggtattgc cgagcggatg
     9061 attgagtctt ttatgttgat ggctaatgaa acagttgccg aacatttcag caagttggat
     9121 ttgcctttta tctatcgaat tcacgaggag cctaaggctg aaaaggttca gaagtttatt
     9181 gattatgctt cgagttttgg cttgcgcatt tatgggactg ccagtgagat tagtcaggag
     9241 gcacttcaag acatcatgcg tgctgttgag ggagaatctt atgcagatgt attgtccatg
     9301 atgcttcttc gctctatgca gcaggctcgt tattcggagc acaattacgg ccactatgga
     9361 ctagctgctg actattatac tcactttacc agtccaattc gtcgttatcc agaccttctt
     9421 gttcaccgta tgattcggga ttacggccgt tctaaggaaa tagcagagca ttttgaacaa
     9481 gtgattccag agattgcgac ccagtcttcc aaccgtgaac gtcgtgccat agaagctgag
     9541 cgtgaagtcg aagccatgaa aaaggctgag tatatggaag aatacgtggg tgaagagtat
     9601 gatgcagttg tatcaagtat tgtcaaattc ggtctctttg tcgaattgcc aaacacagtt
     9661 gaaggcttga ttcacatcac taatctacct gaattttatc atttcaatga gcgtgatttg
     9721 actcttcgtg gagaaaaatc aggtatcact ttccgagtgg gtcagcagat ccgtatccgt
     9781 gttgaaagag cggataaaat gactggagag attgattttt cattcgtacc tagtgagttt
     9841 gatgtgattg aaaaaggctt gaaacagtct agtcgtagtg gcagagggcg tggttcaaat
     9901 cgtcgttcgg ataagaagga agacaagaga aaatcaggac gctcaaatga taagcgtaag
     9961 cattcacaaa aagacaagaa gaaaaaagga aagaaacctt tttacaagga agtagctaag
    10021 aaaggagcca agcatggcaa agggcgaggg aaaggtcgtc gcacaaaata aaaaggcacg
    10081 ccacgactat acaatcgtag atacgctaga ggcagggatg gtcctgactg gaactgaaat
    10141 caagagtgta cgagctgctc gaattaatct caaggatggc tttgctcaag tgaaaaatgg
    10201 agaagtttgg ctgagcaatg ttcatatcgc gccttacgaa gagggcaata tctggaacca
    10261 ggaaccagaa cgtcgtcgta aactcctgct ccataaaagg caaattcaaa aattggaaca
    10321 agagaccaaa gggacaggaa tgaccttagt tccccttaag gtctatataa aagatggcta
    10381 cgctaagctt cttttaggac ttgccaaagg gaagcatgac tatgacaaac gggagtctat
    10441 caaacgtcgt gagcaaaatc gagatatcgc gcgtgtgatg aaagctgtta atcagcgata
    10501 aaaagaggaa ttgaaaatgg aaaaattagt tgcctataaa cgcatgcctt tgtggaataa
    10561 acaaacaatg cctgaagctg ttcagcaaaa gcacaataca aaagttggga cttgggggaa
    10621 aattactgtc ttgaagggag ctctcaagtt tattgaattg acagaagaag gggaagttct
    10681 agctgaacac ctctttgaag caggggcaga caatccaatg gcccaacctc aagcctggca
    10741 ccgagtggaa gctgccacag atgatgtgga atggtacttg gaattttatt gtaaacctga
    10801 ggattatttt gctaaaaaat acaataccaa tcctgttcat tcagaggtcc tagaggccat
    10861 ggagacagtg aaacaaggga aagctttgga tttgggttgt ggtcaggggc gtaattctct
    10921 ttttctagcc cagcaagatt ttgatgtgac ggctgtagat caaaatggac tagctcttga
    10981 aatcttgcaa agcattgtgg agcaggaaga tttggacatg cctgttggcc tttacgatat
    11041 caattcagct agcattgaac aagaatatga ttttatcgtt tcaacagttg ttctcatgtt
    11101 tctacaagcg gaccgcattc cagctattat tcaaaatatg caggagaaaa ccagtgttgg
    11161 tggttacaac cttatcgttt gtgccatgga cacggaggat tatccttgct cggttaactt
    11221 cccattcacc tttaaagaag gagaactggc agactattac aaggattggg aattggttaa
    11281 gtacaatgaa aatccaggcc atttgcaccg tcgcgatgag aatggcaatc gtattcaact
    11341 acgctttgcg accttactag ctaagaaaat caagtaaaca cacatgaaga ttaggaattt
    11401 tcctgatctt ttttcttttt tacgaatgat atagaaaagg agggaattca tgtttgttgc
    11461 gagagatgct aggggagaat tggtaaatgt gttagaggat aaacttgaga agcaagcata
    11521 cacctgccca gcttgtggag gccagctcca tttgcgtcaa ggaccaagtg tacggacgca
    11581 ttttgcccat aaatccttaa aagactgtga ttttttcttt gaaaatgaaa gtccagaaca
    11641 cctggccaat aaggaatccc tctatcactg gttgaaaaaa gagacaaagg ttcaattaga
    11701 gtacccgctt tcagaactta aacagattgc ggatgtattt gtaaatggca atctagctct
    11761 agaagttcag tgtagtccct tgcctcagaa agtccttaaa gagcgaagtg agggatatcg
    11821 tagtcagggt taccaagtac tgtggttgct gggtcaaaaa ctgtggctca aggagcgttt
    11881 gactcgtcta cagcaaggtt ttctttattt cagtcaaaac atgggctttt atgtttggga
    11941 attagacaag gaaaaacaag ttttaagact caaatacctg atttaccagg atctccgcgg
    12001 taaactccat tatcaaatca aggaattttc ctatggtcaa ggtagtttat tggaaatatt
    12061 gcgtcttccc tataagagac aaaaaatatc tcattttaca gtttctgagg acaaggacat
    12121 ctgtcgctat atccggcaac aactttatta tcaaaatctc ttttggatga aagaacaagc
    12181 agaagcctat caaaagggag aaaatatcct gacttatgga ctgaaagaat ggtatccaca
    12241 aattcgacca atagtgggca aatttttcca gattgaacaa gacttgacta gctattatca
    12301 gcacttttat acctattacc aaaaaaatcc tcaaaatgat tggcaaaagc tttatccacc
    12361 agccttttat cagcaatatt tcttgaaaaa tatggtagaa tagaaaggat ggaggaatct
    12421 aatggtatta caaagaaatg aaataaatga aaaagataca tgggatctat caacgatcta
    12481 cccaactgac caggcttggg aagaagcctt aaaagattta acagaacaat tggagacagt
    12541 agcccagtat gaaggccatc tcttggatag tgcggataac ctactagaaa tcactgaatt
    12601 ttctcttgaa atggaacgcc agatagagaa gctttacgtt tatgctcata tgaagaatga
    12661 ccaggataca cgtgaagcta agtatcaaga gtactatgcc aaggccatga cactctacag
    12721 ccagttagac caagcctttt cattctatga gcctgaattt atggagatta gcgaaaagca
    12781 gtatgctgac tttttagaag ctcaaccaaa gctgcaggtt tatcaacact attttgacaa
    12841 gctcttgcaa ggcaaggatc acgttctctc acaacgtgaa gaagaattat tggctggagc
    12901 tggagaaatc tttggttcag caagtgaaac ctttgctatc ttggacaatg cggatattgt
    12961 gttcccttat gtcctagacg atgatggtaa agaagttcag ctatctcatg ggacttacac
    13021 acgtttgatg gagtctaaaa aacgtgaggt tcgtcgtggt gcctatcaag ctctttatgc
    13081 gacttacgaa caattccaac acacctatgc caaaaccttg caaaccaatg ttaaggtgca
    13141 aaattaccgt gctaaagttc gtaactacaa gagtgctcgt catgcagccc tcgcagcgaa
    13201 ttttgttcca gaaagtgttt atgacaattt ggtagcagca gttcgcaagc atttgccact
    13261 cttacatcgc tatcttgagc ttcgttcaaa aatcttgggg atttcagatc tcaagatgta
    13321 cgatgtctac acaccgcttt catctgttga atacagtttt acctaccaag aagccttgaa
    13381 aaaagcagaa gatgctttgg cagtcttggg tgaggattac ttgagccgtg ttaaacgtgc
    13441 cttcagcgag cgttggattg atgtttacga aaatcaaggc aagcgttcag gtgcctactc
    13501 tggtggttct tacgatacca atgcctttat gcttctcaac tggcaggaca atctggacaa
    13561 tctctttact cttgttcatg aaacaggtca cagtatgcat tcaagctata ctcgtgaaac
    13621 tcagccttat gtttacggag attactctat ctttttggct gagattgcct caactaccaa
    13681 tgaaaatatc ttgacggaga aattattgga agaagtggaa gacgacgcaa cacgctttgc
    13741 tattctcaat aacttcctag atggtttccg tggaacagtt ttccgccaaa ctcaatttgc
    13801 tgagtttgaa cacgccattc accaagcaga tcaaaatggg gaggtcttga caagcgattt
    13861 cctaaataaa ctctacgcag acttgaacca agagtattat ggtttgagta aggaagacaa
    13921 tcctgaaatc caatacgagt gggctcgtat tccacacttc tactataact actatgtata
    13981 tcaatattca actggctttg cggccgcctc agccttggct gaaaaaattg tccatggtag
    14041 tcaagaagac cgtgaccgct atatcgacta cctcaaggca ggtaagtcgg actatccact
    14101 taatgtcatg agaaaagctg gtgttgatat ggagaaggaa gactacctca acgatgcctt
    14161 tgcagtcttt gaacgccgtt taaatgagtt tgaagccctt gttgaaaaat taggattggc
    14221 ataaaatggt tgaatcgtat agtaagaatg ctaaccataa catgcgtcgt cctgtcgtca
    14281 aagaagaaat tgtagacttg atgcgtcagc gtcaaaagca ggtcacaggt ttcttgaaag
    14341 aattggaaga ctttgcccgc aaggaaaata ttcctattat tccccatgaa acggttgctt
    14401 atttccgttt tcttatggaa accatgcagc ctaaaaatat tctggaaatt gggacggcta
    14461 tcggtttttc agctctcttg atggctgaac atgcgccaaa tgctaagatt acaactattg
    14521 atcgtaatcc agaaatgatt ggttttgcca aggaaaattt tgcccagttt gacagtcgca
    14581 agcaaatcac tctcctagag ggagatgcgg tggatgtctt atctacactg acagagtctt
    14641 atgatttcgt ctttatggat tctgccaagt ctaaatacat cgtctttctg ccagaaatcc
    14701 tcaaacattt ggaagttggt ggtgtggttg tcttggatga tatttttcaa ggtggtgatg
    14761 ttgccaagga tattatggaa gtccgtcgtg gtcagcgaac catttatcga ggccttcaaa
    14821 aattatttga tgcaacctta gacaatccag aactcaccgc aacattagtg cctttaggag
    14881 atggtattct catgcttcgt aaaaatgtag cagatgttca actgtctgaa agcgaatgat
    14941 tttcagaaaa atttaagaaa aaatagtaaa atagatagag taacacttat ctcaaaggag
    15001 tagacatgaa gaaaaaatta ttggcaggtg ccatcacact attatcagta gcaactttag
    15061 cagcttgttc gaaagggtca gaaggggcag accttatcag catgaaaggg gatatcatca
    15121 cagaacatca attttatgag caagtgaaaa acaacccttc agcccaacaa gtcttgttaa
    15181 atatgaccat ccaaaaagtt tttgaaaaac aatatggctc agagcttgat gataaagagg
    15241 ttgatgatac tattgccgaa gaaaaaaaac aatatggcga aaactaccaa cgtgtcttgt
    15301 cacaagcagg tatgactctt gaaacacgta aagctcaaat tcgtacaagt aaattagttg
    15361 agttggcagt taagaaggta gcagaagctg aattgacaga tgaagcctat aagaaagcct
    15421 ttgatgagta cactccagat gtaacggctc aaatcatccg tcttaataat gaagataagg
    15481 ccaaagaagt tctcgaaaaa gccaaggcag aaggtgctga ttttgctcaa ttagccaaag
    15541 ataattcaac tgatgaaaaa acaaaagaaa atggtggaga aattaccttt gattctgctt
    15601 caacagaagt acctgagcaa gtcaaaaaag ccgctttcgc tttagatgtg gatggtgttt
    15661 ctgatgtgat tacagcaact ggcacacaag cctacagtag ccaatattac attgtaaaac
    15721 tcactaagaa aacagaaaaa tcatctaata ttgatgacta caaagaaaaa ttaaaaactg
    15781 ttatcttgac tcaaaaacaa aatgattcaa catttgttca aagcattatc ggaaaagaat
    15841 tgcaagcagc caatatcaag gttaaggacc aagccttcca aaatatcttt acccaatata
    15901 tcggtggtgg agattcaagc tcaagcagta gtacatcaaa cgaatagtcc aaatcaatga
    15961 gtcagggaaa aaaactcgac ttcaggaaaa aatgaagcaa acattcccac aataaaacgc
    16021 atagtacaag gtttgtactg accccaaaag ttagacaatt aatttatctg aaggatttag
    16081 ttctgtattg cacagggcta agtcctttta gttttacctt aattctctta ttgttgtaat
    16141 aatcaatata gtctataatg gcttgttcca attgattaag tgatttaaat gtttgctcat
    16201 agccataaaa catttcggat tttaaaatgc caaagaaaga ttccatccta tcgttgtctt
    16261 ggctgtttcc tttgcgtgac atagatgctt gaattccctt actctctagg aagcaatgat
    16321 aagaatcgtg ttggtattgc cagccttggt cactatggag aatcgtattc tcgtagtgct
    16381 tctctttgaa tgcctgttcc aacattaacg atcaatcaat ttaatcatgt acctaatatt
    16441 agaattgttt atcccaaatt tatttgaaag cttctctaag ctatatcctt gttttctaag
    16501 ttcatagatc tgaactttat catcataagt taatttcata ataaaaacac cccaaaagtt
    16561 agattttttc tgtctaactt ttggggtgta gttcatgtac acctgatatg atgcgtttta
    16621 taattttaaa gactttttga ccagcctcat ttttttaact tgatactcag tgaaaagcaa
    16681 agattaaact aggaagctag ctgtaggctg ctcaaagaac agctttgagg ttgcagataa
    16741 aacttgtgag gtaaccaaca tatataatgt gaagctgacg tggtttgaat agattttaga
    16801 agggtatgag tctggaagtt ttaatggata atgcaagatt ccatagaatg ggtaagctag
    16861 agttcttatg tgaagagttt gggcataaac ttttaccttt tcctccctac tcatattagt
    16921 atagaaaagt gaatctaaaa tagtacataa ctgcttctaa aacattctta taaattgatt
    16981 taaattctca aatcatatta ttcagttctt atttcatttt gctctacaat cctgttgaga
    17041 agacacgtgt tcatatcaaa aaggtattgg caagttgaaa taccttttta caaggttctt
    17101 ttgtcttatt tttgtttcaa ctgactatat ctcctatggt tctagttcag aaggctaggc
    17161 tataattatg attgataaga agtatcattc caagtattga gagtgaatgt ttcaaaatca
    17221 tgggtttcta taatggtcag gctggcattt gctagaccgc catctttacg aagaagtggt
    17281 tctttatagc ctaggagagt acgaagactg gcagtaagat tggcgccgtg tccgacaatt
    17341 agaatacgct cagctggact atcttttaat gatttgataa attggatggt ccgctgagtt
    17401 gtactataga gggattcggc tccgaacatt cgagtgtcaa attgagcaag atttgaacga
    17461 aaagcctgga tttgttgcgg gtaaatagct tccagggttg caattttcaa accttctaac
    17521 ttcccaagtt gccattcacg gagattagga acgatttcta aagaacaggg ggtatggagt
    17581 tgactttgga taatctcagc agatttgacc gctcgaggta aatcacttga ataaatctga
    17641 tcaaaaggaa tttccttgag atactgacca agtcgtttta gggtttcaat ggattcagga
    17701 agaagaggag aatcaccgct agcaccttga aaacgacctt cttggttcca gagggtacga
    17761 ccgtggcgga caaagtagag tttcattact tgatgtcctc caaaatatct acaaaatctg
    17821 cctttacaaa gctagccaag tcttgtggcg cgacgataat gctgtgtccg acttcgcctg
    17881 cagagacaat cattcgatcc aaatctagag caattttatc gataaaaatg ggataattgt
    17941 gtttctgacg aattccgaca ggattattgg ctccatgaat gtaaccagtt gttttttcta
    18001 agtccttttg tggaatcatg ctcacttttt tattgccaga aattttagct agtttctttt
    18061 cagacaagtg ctgagtgata gggacaattc cgataatcgg tccggtcttg tctcccaaaa
    18121 gcgccaaggt tttgaaaatc tgatctcgtt cataaccttg aggaagctct ccttctaggg
    18181 cattgatttg aatcccctga tgagggatag ctgctttaga taggatttgt tccaccaatg
    18241 tttttttgat tttaactttt tttgccatta tttatattta tcctccaatt gactcatcca
    18301 aataccaagc cagattccca gcgcaaagaa gaaggcgatg atgacataac cgacaagtga
    18361 aagtcctgtg tattggatac tttcagcgtt tcctgcattt ggaattaaga tcaaaagggt
    18421 acttgatagg acgataccga tgatgaaatg atagacgcgt gagtggtagt tgtttaaggc
    18481 ataatccatc aattttgaaa aaacgatgag agttgcacct gcaccaattc caatcggaaa
    18541 gaaggttccc aagagatcaa aagttttaaa accagtcaac ataggagcat agagtcccaa
    18601 aatcaaaagt aaattcgatg ggctgaggcc aggaaccaag acgccaaggg ccaatagtgc
    18661 gcctgctagg acgaagttaa gaaagctggc gcttaaggtt ccaacgacaa aatttaaggc
    18721 atatagtcct aatccagaaa tgataaaggt tgtccataac caagctaaat caatcttgtc
    18781 tcggtcagat tctcgagttg attctttgag gaggctagga actgtaccga taatagctcc
    18841 cgcaaagctc cataatacaa agacctgata attttcaagc aggtattcaa tcgggtagga
    18901 aaataagccg attcccagaa gcataccgat ggcaactgga ataaagtaca aaacattttc
    18961 tttaaggtct ttaaagggat gggccagaaa gccaatcatt cgttcataga ttcctaagat
    19021 tgctgctaga actcctccgg aaattcccgg taggataaat ccaagagcaa ttacaatccc
    19081 tttaataacg cgtgctaacc atgagagcat atatttcctc caactatttc tatttcaaaa
    19141 aatccttact atattgtatc acaatcagac acaaaaagaa aaagcaaatg ataaacaaat
    19201 gcttttaagg ttttaaaaaa agctttcgaa aggtttcttc tttatttttt aagggagaga
    19261 taacgttgat atctaaatcg tggtcaaagc cggcaatttt tcctttagat gtgtattggt
    19321 gaatatcata atctaaatca gttttaggac tgctctccaa aaatcctgag tctgagccgt
    19381 aggacggaat ccaaacagag gtaaacttgc ctgtatcaat actgtgttct tccatgaagt
    19441 agacaccaac gtagatgccg atgtttttag cacctagtga tgctagtttt gctcgaaagt
    19501 tttcgacacc ttcgttcata ttagacatgg ttttgtcttc cacgtcaagc caatagtaac
    19561 tagggctgta aggagaggca gcattgtaga aaacttcggc agccttttcc atttcttgga
    19621 cactttttcc agctacataa gcgtagacag caactgggac attccgcttt tgaagttcag
    19681 tgatatgact cttataggcc ttgtctattc cattgataaa tgaagcatca ttttcttttg
    19741 tcgtttgagc accactgtga acacgaacaa tagcacctga aatattttgt gagagggcat
    19801 cgtagttgat ttcctcagga cgctgccagc cagagaggtc aataatcggt ttgtctaagt
    19861 gtttcaaagc ctgtgcttca atctgtgcta tattggattt tgttttaaac gattggttgt
    19921 cattaagtgg gcgattgatg attaaaatga acatcataat cccaaaaaaa ctaaataaaa
    19981 taagtggatg aatttgtttt ctcatatctt ataattctac cctaaaaatc aaaaaaaatc
    20041 aaaaaaatgg gttaaggaag agactttaga gcattttttc attcaagagt gcggaatgat
    20101 ttgaaatatg gtataataaa agggaatttc tacagaaaag agaagattat gtcaaatttt
    20161 gccattattt tagcagcggg taaagggact cgcatgaaat ctgatttgcc aaaagttttg
    20221 cacaaggttg cgggtatttc tatgttggaa catgttttcc gtagtgtggg agctatccaa
    20281 cctgaaaaga cagtaacagt tgtaggacac aaggcagaat tggttgagga ggtcttggct
    20341 ggacagacag aatttgtgac tcaatctgaa cagttgggaa ctggtcatgc agttatgatg
    20401 acagaaccta tcttagaaag tttgtcagga cacaccttgg tcattgcagg agatactcct
    20461 ttaatcactg gtgaaagctt gaaaaacttg attgatttcc atatcaatca taaaaatgtg
    20521 gccactatct tgactgctga aacggataat ccttttggct atggacgaat tgttcgtaat
    20581 gacaatgctg aggttcttcg tattgttgag cagaaggatg ctacagattt tgaaaagcaa
    20641 atcaaggaaa tcaacactgg aacatacgtc tttgacaaca agcgtttgtt tgaggctttg
    20701 aaaaatatca ataccaataa cgctcaaggc gaatactata ttacagacgt cattggtatt
    20761 ttccgtgaaa ctggtgaaaa agttggcgct tatactttga aagattttga tgaaagtctt
    20821 ggggtaaatg accgtgtggc gcttgcgaca gctgagtcag ttatgcgtcg tcgcatcaat
    20881 cataaacaca tggtcaacgg tgttagcttt gtcaatccag aagcaactta tatcgatatt
    20941 gatgttgaga ttgcttcgga agttcaaatc gaagccaatg ttaccttgaa agggcaaacg
    21001 aaaattggtg ctgagactgt tttgacaaac ggtacttatg tagtggacag cactatcgga
    21061 gcaggagcgg tcattaccaa ttctatgatt gaggaaagta gtgttgcaga cggtgtgaca
    21121 gtcggtcctt atgctcacat tcgtccaaat tcaagtctgg gtgcccaagt tcatattggt
    21181 aactttgttg aggtgaaagg atcttcaatc ggtgagaata ccaaggctgg tcatttgact
    21241 tatatcggaa actgtgaagt gggaagcaac gttaatttcg gtgctggaac tattacagtc
    21301 aactatgacg gcaaaaacaa atacaagaca gtcattggag acaatgtctt tgttggttca
    21361 aattcaacca ttattgcacc agtagaactt ggtgacaatt ccctcgttgg tgctggttca
    21421 actattacta aagacgtgcc agcagatgct attgctattg gtcgcggtcg tcagatcaat
    21481 aaagacgaat atgcaacacg tcttcctcat catcctaaga accagtagga gcctatcatg
    21541 gagtttgaag aaaaaacgct tagccgaaaa gaaatctatc aaggaccaat atttaaactg
    21601 gtccaagatc aggttgaatt accagaaggc aagggaactg cccaacggga tttgattttc
    21661 cacaatgggg ctgtctgtgt tttagcagta acggatgaac aaaaacttat cttggtcaag
    21721 cagtaccgca aagctatcga ggctgtctct tacgaaattc cagccggaaa attggaagta
    21781 ggagaaaaca cagcccctgt cgcagctgcc cttcgtgaat tagaggaaga aacagcctat
    21841 acagggaaat tagaactctt gtacgatttt tattcagcta ttggcttttg taatgagaag
    21901 ttaaaactat atttagcaag cgatttgaca aaagtggaaa atccgcgtcc gcaggatgag
    21961 gatgaaacct tggaagtcct tgaagtgagc ttagaagaag cgaaagaatt aatccaatca
    22021 ggtcatatct gtgatgccaa gacaattatg gctgttcagt attgggagtt gcagaaaaaa
    22081 tagaggaggt cagtatgggt aaatctttat taacggatga aatgattgaa agagctaata
    22141 gaggcgaaaa aatttcaggt cctcctttgc tggatgataa tgaggaaact aagattttac
    22201 caacctcttc ttcccgtttt ggttatgcca atcctaagga tcatggtttt agccaggaaa
    22261 ccttgaagat tcaggtcgaa ccatctattc ataaaagccg tcgcattgaa aataccaaga
    22321 gaaatgtctt caattctaag ttgaataaaa tcttattcgc ggtcatcttt ctcttgattt
    22381 tgcttgtttt agcaatgaaa cttttgtaat agaaaaggaa ttgaaatgaa aataggaatt
    22441 attgctgcta tgccagaaga actggcttat ctggtccagc atttagacaa tgcccaggag
    22501 caagttgttt tggggaatac ctatcataca ggaaccattg tttctcatga agtcgttctt
    22561 gtagaaagtg gaattggtaa ggtcatgtct gctatgagtg tggcgatttt ggctgttcat
    22621 ttccaggtgg atgcccttat taatacgggt tcagctgggg cagtagcaga aggtatcgct
    22681 gttggggatg tcgtgattgc tgacaaatta gcctatcatg acgtggatgt cacagctttt
    22741 ggctatgctt atggacaaat ggcgcaacaa ccgctttatt tcgaatcaga caaaaccttt
    22801 gttgctcaaa tccaagagag tttatctcaa ttggaccaaa actggcatct tggtttgatt
    22861 gctacaggag atagttttgt tgcaggaaat gacaagatag aagcgattaa gtcccatttc
    22921 ccagaagttt tagccgtgga gatggagggg gcagctattg ctcaagcagc gcatgccctc
    22981 aatctcccag tcttagtcat cagagctatg agtgacaatg ccaaccatga agcaaacatc
    23041 ttttttgatg agtttattat cgaagctgga cgtcgctctg cccaagtctt gttgaccttt
    23101 ttgaaggctt tagattaagc ggaaatttga cagtttttct agcttatgat aagatttaag
    23161 taaagaaaag ctagaaaacg tttcagagga tattatgagt attgaaatga ccgtcagtga
    23221 gattgcagag gtcttaggat tatctcgcca agcaatcaat aaccgtgtca aagaattacc
    23281 agaagaagac acagataaaa atgacaaagg ggtaacagtt gttaccagaa gtggcttgat
    23341 taagctagaa gaaatctata aaaaaacgat ttttgaagat gagcctgtca gtgaagatgt
    23401 caaacaacgt gaactgatgg agattctagt ggatgagaag aatgcagaaa ttcttcgtct
    23461 ttatgaacaa ttaaaagcca aggatcgtca gttatcagaa aaagacgagc agatgcgtat
    23521 caaagaccgt cagattgctg agaaagacaa acaattagat cagcaacaac aattgaccct
    23581 tcaagccatg aaggatcaag aaaatcttaa gctagagctg gaccaagcaa aagaagaagt
    23641 ccaatccact aagaaaggct tttttgctcg tttatttgga ggataatcaa ggagctgttt
    23701 aggttaaatg ctaacctgat ggcttctttt gtttgtgaat agtagaggag aggtacagaa
    23761 tggaacgatt agaagattac attgcttttg acttagaatt taatcagcac gaaggacaaa
    23821 cacacttgat tcaagtatcg gcagtgcgtt ttagagatgg tcgggaaatc gatgcctatg
    23881 attcctatgt tcataccagc gtccctctaa aaagctttat caatggtttg accgggatta
    23941 cagctgagac cttaaaagat gctcctccag ttgagaaagt cttagaagaa ttccaagagt
    24001 ttgtgggttc tttgctgatg ataggctaca atgctgctaa aagtgattta cctattttag
    24061 cagagcatgg tttagactat agccaacagt acaaggtgga tttgtatgat gaagcttttg
    24121 aacgtcggag ttcagattta catggtattg ccaatctcaa attgcaaact gttgcctcat
    24181 ttcttggatt tcaaggtcag tctcataata gtttagagga tgctcggatg acggcgcgtg
    24241 tttacgaatc cttcttagag tcggatcaag ctagggaatt gttagagaca gaaagtagct
    24301 tatcaaacaa tccttttggt gtcttggact tgtctcaatt atttgactag gagtgcctat
    24361 gaaacctgaa aaacctaaaa aattgggtct taaacagatt tacctcaatc ttgataaaat
    24421 cctctttcta tttttcttga tttttatgat agtggagtat ttgtgggtac cattcaattc
    24481 atggttggct agcttcttat tagatcagac aggttatctt tttatttcct ataacaatgt
    24541 cttagccatc cttactcatt ctccattgat tagtctagct tttttagcct tgattgtaat
    24601 taaccttctg gttgcttatt ttcaaatagg actcctcttt attggtgctc gtcatctcct
    24661 ctataatgaa aagcgaaggc taattgagta tatccgcaag gttttccatg aaagttttgg
    24721 atttatgaaa aagctaactg gcgctaaagc cttgtttatc tttttctata tagctatgct
    24781 gtttcctttt atacggaaaa tgctgaagat ttattatctt aataaaatcg tcattccaga
    24841 gtttatccaa gcttatttag aagatagata ttggatgtgg tggctgagca tcctacttct
    24901 atctcttatc tttctctatg tctctgtcag attgatgttt gcccttccga agattgtata
    24961 tgatcagctg actgttcgag aagcagtgat gtttagcttg gagaaaacca agaagagagt
    25021 tactttttat gcttggaatt tattttacat cttgctcaaa gcacatttat tattttatct
    25081 tcctttgatt cctttattat tggctcaaac tttggtagat gatatcactc agagagagtc
    25141 tctgattctt ggtattttga attttgtcgt gattaaaaat ctctattata tggctctgac
    25201 ttattttttg gttaaatttg tttcattttt gacaggaaag gagctggata tgcttcctag
    25261 gagagaaaaa gatcacatcg tgcgatgggg tgtcatgact tgtgccagtc ttttctttgc
    25321 cttagaaggt tatatttatc tggaggctcc catggttcat ctacctcaac ttatttctca
    25381 ccgaggggtt tccaatgcaa atgggattca aaatacagta gagtccttgg aaactacagc
    25441 acaactcaaa ccagacttgg tggagacgga cgtgcaggaa acaaaagatg ggcagtttgt
    25501 catgatgcat gatgctaact tgaaaaatct agcaggtatc aataaaagtc ctcaagactt
    25561 aaacttggag gagcttaaag ggattgatat ttttgaaaat ggctaccaga ctaaaatttc
    25621 aagctttgaa gattatctca gtcgagccaa cgaacttggt caaaaattac taattgaaat
    25681 taaaaccagt aaaaaagata gtccagacat gatgaaccgc tttttagccc gttatgctgc
    25741 aaagctcaag atttatggac atcaaatcca gtctttagac taccatgttg tcgaaaaagt
    25801 aagacagtat gatgccgaac tgccagttta tttcatcatg ccctacaatt ctgtctttcc
    25861 taaaacaaga gcgacaggat atactatgga gtactcaacc ttggatgaat attttgtaag
    25921 caaactatgg acaacggatc agaaacttta tgtctggact gtcaatgatt cagaagctat
    25981 cagcaaatct cttcacttag gagtagatgg agtgattaca gatgatttgg aaaaagttca
    26041 gcgggaaata gaagtagccc aagaagatcc agaatatacg gatttgctct tgaaaaaagc
    26101 attggaattc tttgaatttt agtaataaaa aagaggaacg gttgttcctc ccatgatagg
    26161 taagtattct tgtgaatgct tgcttttttg tggttgcaat ccgtttacga gaattgtttg
    26221 tattgttctt gtttcaattt actttatagt gctttgaagt tggaatgcta cactataact
    26281 tataaa
//
LOCUS       DAVBDG010000027        26219 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBDG010000027 DAVBDG010000000
VERSION     DAVBDG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.027"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(223..519)
                     /locus_tag="VQ526_001094"
     CDS             complement(223..519)
                     /locus_tag="VQ526_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554826.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(503..2662)
                     /gene="pfbA"
                     /locus_tag="VQ526_001095"
     CDS             complement(503..2662)
                     /gene="pfbA"
                     /locus_tag="VQ526_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2554827.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHTIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SVIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3792..5018)
                     /locus_tag="VQ526_001096"
     CDS             complement(3792..5018)
                     /locus_tag="VQ526_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2554828.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(5061..5753)
                     /locus_tag="VQ526_001097"
     CDS             complement(5061..5753)
                     /locus_tag="VQ526_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2554829.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(5939..7690)
                     /locus_tag="VQ526_001098"
     CDS             complement(5939..7690)
                     /locus_tag="VQ526_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554830.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7680..9425)
                     /locus_tag="VQ526_001099"
     CDS             complement(7680..9425)
                     /locus_tag="VQ526_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2554831.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9553..9927)
                     /locus_tag="VQ526_001100"
     CDS             complement(9553..9927)
                     /locus_tag="VQ526_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2554832.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKP
                     GVLYSSKISSNRVNVALPYVGY"
     gene            complement(10080..10536)
                     /locus_tag="VQ526_001101"
                     /pseudo
     CDS             complement(10080..10536)
                     /locus_tag="VQ526_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10669..11496
                     /gene="xth"
                     /locus_tag="VQ526_001102"
     CDS             10669..11496
                     /gene="xth"
                     /locus_tag="VQ526_001102"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2554833.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAKNADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11508..11651
                     /locus_tag="VQ526_001103"
     CDS             11508..11651
                     /locus_tag="VQ526_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2554834.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            11651..11890
                     /locus_tag="VQ526_001104"
     CDS             11651..11890
                     /locus_tag="VQ526_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2554835.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLYQRVMKANYPI
                     YRPLTKRKFRVSDPYIYPHEFAVLDPDGYFLRFSE"
     regulatory      12273..12368
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12768..13349
                     /locus_tag="VQ526_001105"
     CDS             12768..13349
                     /locus_tag="VQ526_001105"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2554836.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGRVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13349..14611
                     /locus_tag="VQ526_001106"
     CDS             13349..14611
                     /locus_tag="VQ526_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2554837.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14698..15564)
                     /locus_tag="VQ526_001107"
     CDS             complement(14698..15564)
                     /locus_tag="VQ526_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2554838.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(15551..16321)
                     /locus_tag="VQ526_001108"
     CDS             complement(15551..16321)
                     /locus_tag="VQ526_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2554839.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(16347..17201)
                     /locus_tag="VQ526_001109"
     CDS             complement(16347..17201)
                     /locus_tag="VQ526_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2554840.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(17358..17762)
                     /locus_tag="VQ526_001110"
     CDS             complement(17358..17762)
                     /locus_tag="VQ526_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2554841.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERQVRI"
     gene            complement(17832..19313)
                     /locus_tag="VQ526_001111"
     CDS             complement(17832..19313)
                     /locus_tag="VQ526_001111"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2554842.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(19332..20510)
                     /locus_tag="VQ526_001112"
     CDS             complement(19332..20510)
                     /locus_tag="VQ526_001112"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2554843.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAFDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEVFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            20674..21681
                     /gene="galR"
                     /locus_tag="VQ526_001113"
     CDS             20674..21681
                     /gene="galR"
                     /locus_tag="VQ526_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2554844.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(21727..22764)
                     /locus_tag="VQ526_001114"
     CDS             complement(21727..22764)
                     /locus_tag="VQ526_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2554845.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(22765..23118)
                     /locus_tag="VQ526_001115"
     CDS             complement(22765..23118)
                     /locus_tag="VQ526_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2554846.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(23362..24252)
                     /locus_tag="VQ526_001116"
     CDS             complement(23362..24252)
                     /locus_tag="VQ526_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2554847.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            24385..24921
                     /locus_tag="VQ526_001117"
     CDS             24385..24921
                     /locus_tag="VQ526_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2554848.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(25087..25893)
                     /locus_tag="VQ526_001118"
     CDS             complement(25087..25893)
                     /locus_tag="VQ526_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2554849.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLPN"
BASE COUNT         7875 a         5771 c         4641 g         7932 t
ORIGIN      
        1 aaataagtta tctactctat gatcagttat ttccatttcg tttattaaca tgacgcagtc
       61 acctagcatc atatactcca actttttttc tgaaagttta gcaaatataa aagcaaatat
      121 tggtaagcga taatatagta tattgaaact agaatagtac acctctactt ctaaaacatt
      181 gttagaaatc gatttgactg tcctgattga tttgtcctat tattatttca ttttactata
      241 ctctgttaat tcatatgagt ttaaaccgat ttcatcttta acctcgagta aagcagtttc
      301 aaatatttgt ttaagagttt ttgattcttt acaattaacc gacaaacttt ctgataaaat
      361 atgtacaact tctgagactg aataacctat ctcctcttta gaattatata aatctgtagc
      421 tccaccaata atccaaaaat actgattttg tgaacctaca atatcctcat tttctacgga
      481 acttccttgt atcgaacaaa ttttatttat ctttaccata atacttcaac ccttttagtg
      541 tcaaaagtaa accaattcct gtcactgtta agaatagttc cataatcttg ttcgaaccag
      601 tctttggtaa tttttgtttt acatctacta tttctttaga tttattaata tgattttcag
      661 tttctctgcc atctccaact attttatagt ttacttcttc tgtcttatta tcttgtttat
      721 tgtcgatctt gtcattcatt tgtctattat ctttacttga gttaaactct ccgttcttct
      781 ggttactatc aattacatta tttgaattag attgtttttc ctctttgttt ttttcttttt
      841 cgtttttatc acttaaatta tttgttacaa ttttgtaaag cccattctcc gttacaatat
      901 tgaaattacc atcgctatca cgtataacag gttctttccc atttgcatta gatttgatga
      961 ctgatatata cttaccggat aaattataaa attggttatt taaaacggtt attttaccct
     1021 ttgaatcctc aataacaatt ccttctttac caccgaatat actattctct gagactacta
     1081 aattatcact aactcgtaat aattcaatat ttggttgttc tgtttcctta gaattattat
     1141 taattacatt ttttgttaca gtaatatctg atacttttcc taaatattct gcactatctt
     1201 ttgcaactcg tatcgctttt gttttaggat cggcaatatt aaatatattt tcggcgataa
     1261 ccacctgttt atttaaatct aataggtctt tagtgttttt atagctataa gcatttacta
     1321 aagctgctcc gttttctcga taatgtacac tctctccttt aactttctta tcaaagcgat
     1381 ttcctttgat taatacatca gtgaatcctg taaaacgtac acctgcatac atcatgttat
     1441 caaaatgatt atttaaaatt ttaatattag aggggttctg tgtcgacaat gtttgatagt
     1501 gtgtaccaat tgctgttact aattccccag atttatcact tttgccaaaa taggaatttt
     1561 gaatagtcac attttcagat tttttcccat catcattcaa ggcataagga aaaccttttc
     1621 tagttaatgg ttcaatctga atgctctcct tacttatgat ttgcccatcc ttcatcgttt
     1681 tgggtaaggc ttgcccaaga aaacgagaat tatcaactaa tacatttttc gaacctgcaa
     1741 tttgaatagt atgcccttga taactatcct tgaatgttac attttttata gttacgttat
     1801 ttgaattccc aatagcaaat gcacctgaag aatttataag tggtagattt tttgctttag
     1861 ttccttcttc attcaaagca ccgttcatat caatcgtacc accagaataa ctaatatctt
     1921 ctgttgggcc ccattctact tgcgcaccat catccgtaaa taaacctgtc ataaaaacaa
     1981 tggaagggtg attcttaata tttataccat ttagaattgt agctttctca ttcaattcta
     2041 agtgtgtatg actttttaaa aaaacaattt cttttactaa ataagttcct tcaggaaaat
     2101 atacatttcc tccacctagc ccttgagctg cagcatctat tgcatcttga attgcttgtc
     2161 gatcatcatg aatcccatca cctaccgcac cataatcttt tacacttaaa gcagtatctt
     2221 caaattcttt tttgataatt ctgtcactta taacatcttc cttataactc ttattttcat
     2281 tagtagatgt tactgtagat tcaactgttc tgttgtctaa ttgtgaattg gaacgaatag
     2341 taccattaga gctgatacta ttaacactta acgaattatc taaaccatta gtggttgaat
     2401 tagaggcaat catttcagca gaacgattct gattaacagt tggtgaatta tctaaatcat
     2461 tagtaattgc attagaagac tcttttgtag ccatcggtga agaactagta acaacttcat
     2521 ccgataaaat accttggctt cccaaaattg aaattgccaa tagtaccgag caagtcccca
     2581 cctttaattt acgaatactg aatacctcat taggaacaaa atatttcatc agaacctcca
     2641 atttttttac tatttttaac aattgattat tacttttctt aaaatagata gaatgacacc
     2701 gctgtcatta tctttatcat atattttcca aattgaattg tcaaggaata acacaagaaa
     2761 atattaaaat ttatactctt cggaaatctc ttcaaactag gtcagtgccg ccttgcccta
     2821 agtctaggtt actgacttgg tcagttctat agtaaattga aactaaaata gtacacctct
     2881 acttctaaaa cattgttaga aatcgatttg actgtcccga tcaatttgtc ctattcttat
     2941 ttcattttac tatatctaca acctcaaagc agggttttga gctatctatg gccaacttcc
     3001 tagtttgctc tttagccttc cattcaaaag tgatgctatc tttcttagct tgaatataaa
     3061 atatcccatt tactatattt aaatttattg atatgttgta aattataaat gaaagaacaa
     3121 ataccttgtc atacaagcat tcgccaggga tatttacatg aatatcgctc atatggcgga
     3181 ttgaaactaa aacactacaa tatatttctt aaatagacct tctataaaag ctagatttaa
     3241 cctcgataaa aatagatgca gtgcttctaa aatactggaa tagggcatat aggtaatagt
     3301 agacaagtgt tttatagatt gtctcgcggg agatctaatg atgtatagca agcatcacta
     3361 aacatataga atatttgtgt tattataata tcaatatgct ataaattcta tcatgcaaaa
     3421 gaatgactag aacatgaaag acaggcacaa aactttgaac aatattctga aaacaaaaaa
     3481 agacgcgagt ttccacaatc tcactcatgg aaactcacgt ctttcaaaaa atagggtgaa
     3541 ttaaaaacta gaacaagaca ctagattttc taaatgatgt ctagtaagga ttctaaatgc
     3601 tacaccaaat tgctcacatc ttatttcaaa ccacgggact cccacaagaa aggaaaatcc
     3661 tataaaaatt ctctctctga ggagagagag gcctcaacta caggtgtaaa ctattatcaa
     3721 ttgaaaacaa cagtttttac actcaattag actttttagg gacaggatca ctttcgcttg
     3781 actgtagttt gtcatttttt tgataaagtt agcacttttt cagaaactgt tttgaaagtc
     3841 tcaatgatat agtctacttc ttcatcgctt aatttagtat gaagagggag ggtaatttca
     3901 ttctcaaaga aggcataggc cttaggatag ttcgtcatat caaatccaag attcttatag
     3961 gctgtcaaga gaggaagcgg tttgtagtga acattacttg caattcctgc tttagccaat
     4021 tcttggatga tgaggttgcg ttcttctagg cttgctcctt ctacacgggt gatgtagagg
     4081 tggcgtgaag attcgacagt ttcagtcttg tgtgccaaag gatggatgcg agaacctgca
     4141 aaaccactat catagcggtc cacaatatcc ttacggcgtt gcaacaaact tggatagcgg
     4201 tccaattgta ccaaaccaag tgaagccatg atatcggtca tgttgcactt ataggctggt
     4261 gtaacgatat cgtattccca tgaccccagt tgcatcttgg caagagcatc cttagtttgc
     4321 ccgtgaaggg aaaggatttg gaattccttg tacatctctt cgtcatcaat cactggattg
     4381 gctttccaag tcgcacttcc accttctgcc gttgtaaagt tcttaacggc atggaatgag
     4441 aaggaagtaa agtcagcgat agaaccagaa ggttgtcctt tataagtaga tcccaaagcg
     4501 tgggcactat cagagacaat gacaatacgg ttaaaggcct tttgccactt gcttgaagcg
     4561 gtaaagaagt cacgtttttt ctccacgact tggaacaaac ggtcataatc gcaaacaatc
     4621 cctgcgagct ctactgggat aatcacctta gttttctcag tgatagcttg ctcaagcagg
     4681 tcatagtcca tctcaaacgt atctgcttgg atatccacca tgacaggggt tgctcccacg
     4741 tgcgtaatga cactacatga agccgtatag gttatggctg gaacgatgac ttcatcacca
     4801 ggtcccactt caaaaacgcg taaaatcaac tccagagcgg ctgtcgcaga gttgagacaa
     4861 acagtcttag gtgtctgtgt gtaaagagac aagcggcgct ccagttcttt tgttttagga
     4921 cctgttgtga tccaaccaga acgcagggta tccgctactt cagcaatttc tgcttctgtg
     4981 atatcaggcg gtgaaaatgg aatattgtaa tttggcattg atttgctcct tttatctgta
     5041 ctcattctct tatgactact ttattttagt accttaaaca cggtttgaaa catgattttg
     5101 atgtccccaa agaaactaaa ctctcggaga taggcgaggt tatagcgcat cttttcagga
     5161 agaacatgct ccacataggc ctgatcaact gacagacctt tctctgtcat ttgactgatg
     5221 atggtgtcct catccttgta gttgatgctg gctggagagg taattcctgc ttgcaagagc
     5281 aaggttgcca tcatttcagt gctatactgc tctgtataac gtggcacttc aggtcgtgta
     5341 ccgacaaagg acatctcacc tttaaggaca ttgaccaact gaggcagttc gtccaaacgg
     5401 acacgtcgga tgaaatttcc aaccttggta atacggctat cgttagcaga agtcaccaga
     5461 tttccttttt tatccgcatc cgtcaccatg gtacggaact tccaaatctt gaaccgacgg
     5521 ttgtactggg tcacacgctc ttgcttgtaa atcactggcc ccttgctatc caacttgatc
     5581 caaatgctca agatgagaaa gatgggagag gtcagaacca gtaagaccaa ggccaaaacc
     5641 cagtccaagc aacgcttgaa aatcagcgaa cccttccttt tagagacaag ctggtagtaa
     5701 gactcaacct cgcttgattt catttccaca ggcaagtctt cccatttcag catgctgttc
     5761 tcctttgttt ttattatact atttgtcaac actttttgtc aacacttttc attataccac
     5821 aaaatggaaa aggcggtgaa aaaaatctgt aatttgaagg atttccttct agtactcaat
     5881 gaaaatcaaa gtacagcttt gagattgcag ataaagctga catggtttaa agagattttc
     5941 aaagagtata ggccatggcc cctgcctgca aactatacat cttgtgatag gttcctccca
     6001 gagccaagag ttcctcatgg gttccactct cgataatgcg tcccttatcc aagacataga
     6061 tgcagttggc gtcttgaata gtagaaaggc ggtgagctat agcaatagtt gttcggccct
     6121 gtctcatctt cgccagagaa gcttgaacca agctttctgt ttcagagtca atattggctg
     6181 tcgcttcatc caaaatcagg attttaggct ggctggcgac tgttctagca aaggcaagaa
     6241 gctggcgttg cccagtagag aagctcgaac cacgctcgga aacaggggag tcgtacccct
     6301 gaggaagttc ttgaataaag gaatctgcat ccacaaaggc tgccgcagcc tgaacctgct
     6361 catcactgat ttcttggtac atggcgatat tggacttaat agttccatga tagaggaagg
     6421 gttcctgcaa gaccaaaccg atgttttttc tcagctcttc ttgactgaaa tccctgatat
     6481 ccacatcatc caagagaact ctccctgact ggaattcata aaagcgcatg aggacattga
     6541 taatagacga tttccctgaa cctgtatgac ctacaaaggc aatggtttca cccttattaa
     6601 cagagaaaga aatgtcatcc agaatcggat gtttaccgtc atatgagaaa cacacatgtt
     6661 caaagcggat attgccttct tggactttgg cttgcccatt ttcttgaaga ggttcatagg
     6721 tcctctcgtc taccagggca aagacacgac ctgcagaaac catagacgtt tgcagtgttg
     6781 aaaagttttg cgtcacctca atcaaagggt caaagaggcg gttgatgtac tggataaaag
     6841 catacatggt cccggccgtt atcccgatag aaagaccacg atagccaaaa taggccatca
     6901 agacagcata acctagaagt ttcaacaaac tcatggcagg tctcaaaaag agggcatcca
     6961 aggctacaga acggttggca tagaccaagt gctcttggtt gatttcatca aattctgcct
     7021 gcaggcgctt ctcttgatta aaggcctgga ttattctgat tccctcgatg ttttcagcca
     7081 gcttgctatt gatatctgac aagagacttc tggttttctc aatgattttc actgattttt
     7141 tccgatagag attgaccaaa aggaaaatca aggggagaaa gagcaagact aatgctgtca
     7201 aacgaaaatc caataccaac atggtataaa gggttgtcag aaagatgaaa actgctgaga
     7261 taaagctaga taaaatccca gaaaacatat cactaatcgt ctcggtatcg ttggtcaaac
     7321 gagaaacgat agaacctgct ggcgtcttgt caaagtaaga catgcccagt ttctccatat
     7381 tggcaaaggc atcccgacga atatccctaa caatactgta agacacgcgc gcaaagagaa
     7441 gattgccgac atactgaact acagtttgta ggatgtagag accatagtag accagcaaaa
     7501 cggtaacggc tagttggtta agattgctga gatactggtc gataaagtgg gaagccacga
     7561 ggggtatgac acttttaatg accgtcgtcg ctaggagaaa actgagtgcc aaaaaggtca
     7621 ggagtctata aggcttgaga taagacatca agcgcttcaa tacagcccat tgttcttgtt
     7681 tattctgcat cttcttctcc tttcatttct aactgctgag actggtaggt ttgggcatac
     7741 cagccatcca tcgctagcaa gtcttcgtgc ctgcctcgtt cgataatttg accattttgt
     7801 agaactaaaa taaaatctgc atggacaaca gcactgaggc gatgggcagt tataatggtt
     7861 gtcttgtcct ttcgcgtctc cttgaggttg tcgataatcg catactctgt cttggcatct
     7921 acggcggata aggaatcatc caaaatcaag atatcagggt ttaaaatcat agcccgactc
     7981 atagccaacc gttgcttttg accaccagaa agactgactc ctttttcacc aatcagcgta
     8041 tcaaatcctt gaggcatgtc tacaatatct tggtaaactc gggctagctt agtagcttcc
     8101 tcaacctctg aaaggggcaa gttaggattg ccaaagcgga tattgtctag gattgaagtc
     8161 gcaaagagaa actggtcctg aggaacatag cccatgagac tgcgaagttc tgtcaaacga
     8221 tagtcccgaa tatcgtgacc gtttagataa atggcaccct tatccacatc gtattcacgc
     8281 aagaggagct tgattaagga cgttttccca gagcctgtct gcccaaccaa gcccagtgtt
     8341 tgcccttttg ccaaactaaa gtgaatatcc gtcagtgttt cctcattttc aaaagcaaag
     8401 ctgtcaatgg catactccaa acgcccattt tcaataccat ccagaggaaa ctcagggtct
     8461 tgtacaggag attcctgaga caaaagattt tcaatccgct ggtaagaaac cttccctcgc
     8521 tgagtagtat taaagaggaa gccgatggcc ataagaggcc agaccagcat atccaaatag
     8581 ctgataaagg tgactagatt accaactgta atctgccctt cctgaaccat caaggagcca
     8641 accaaaagcg ttaaaacata ggacgaacca acaaacaaga gaaccatagg gtcaaagaga
     8701 ctatcatatt tcatggtttg caggttcttt tggaaggtta attcattgac tgcctgaaaa
     8761 gacttcaact cgtctgcctg ataaccgaaa gacttggtca ctttgatacc tgatacggac
     8821 tcctgtacct tgttattgag ttcagaaaaa gcagcttggg attcgccaaa ggccttatga
     8881 gtctttctcc ctaggcgact agtcgcatag gccatgaaag gtagggggag aatggcaaca
     8941 agagtcatct gccatgagat gctaaagagc atggtcaaca aagtcaccag agccgtgata
     9001 gaggcatcca ccgcagacat gacaccgcca cctgctaaac gagtcaaggc attgatatca
     9061 ttggttgcgt gtgccatcag atcacccgtc cgataggttt gataaaaggc tggcgacatt
     9121 tttgtgaaat gcttaaacaa gcgagaccgc atgatctgtc ccaagcaata agaggtccca
     9181 aggatataca tacgccacac atagcgcaaa tagtacatac caaaggctgc aagtagcaag
     9241 taaaataggc taagaaggag gtcctgctgg gttaattgcc ccgatgtgat ggcatcaatg
     9301 actcgcccca taaccatagg aggaatgaga ttgagaacgg aaaccaagac cagggccaca
     9361 atcccgacta gataacggtg tttttctaac ttgaaaaacc accagagttt ttgaataatg
     9421 gacataaaat ccctttctga ttgcaaatgg aaacctgagg ccaatactca atgaaaatca
     9481 aagagcaaac taggaaacta gccgcaggct gctcaaagca ctgctttgag gttgtagata
     9541 gaactgacga agtcagtaac ctacatacgg caaggcgacg ttgacgcggt ttgaagagat
     9601 tttcgaagag tacaagaccc caggtttttc tttttcataa gttacgactg taacagcacc
     9661 cttgtcatat tcagcaataa agatattggc tacattgtca tgcccttgtt tgctgaggtt
     9721 atcaagcaac cactcttcgc tacgaccaat cgattccaag acgtctactt gaatcacacc
     9781 gtcagtcaca actggatact taggattttc atctcccatt tgcacaacga tgagttgccc
     9841 attttgctct tgcacagctc gtttgacttg cttcatctgg aaaatccctt ggctacgaag
     9901 tttgagggct acatccgatg cagacaaacc aactgaacga caggcttatg ggtcaatatt
     9961 cccatttttg ataaggagag ttggcttacc atcaatcaaa cgtttcacaa agtgaacatt
    10021 gttgttgagc tatttgagag tcaagatcag tttgattaaa atttcgatat aattaagtgt
    10081 catttgttca catccaccag ttcaattgcg tctgtcttat acaattcgac tttctctaac
    10141 aggtacttgt ctggctcact tccgtttagg gcacgataat agcgatctcc caccttgata
    10201 agtgcgccat ctgtgtttgt ggaagtatta acatggactt ctgacttgtc tactcccaaa
    10261 tctttggaaa caacctcgat gaaatgaagt gaagtttgaa atttattgtt agaaacttga
    10321 ttgttttgat aagtcgtgat gctgaccaaa agcatagcta aataaggcca gagctgaaat
    10381 catgaccaac tcacgaaatt tggttccttt tttatcatgg tatgccttga aagcaaaaaa
    10441 aactgtcaca gctaatagaa caatcccaaa gccaaccatg ataccatttt gctgaccgat
    10501 ttggctgagt acatagtcat aagagtagaa tttcatgtat tttcactccc tttcataaaa
    10561 tcattcttaa ttataaacga aagagagtaa tttttcaaac catacgttgg ggaaatagct
    10621 ctttttttgg tataataaaa tctataatct gaatgaaaag gtaactttat gaaacttatc
    10681 tcatggaata ttgattccct aaacgctgcc ctaactagtg actcagctcg tgccaaattg
    10741 tcccaagaag tcctacaaac cttggtcgct aaaaatgctg atattattgc tatccaagaa
    10801 accaagcttt ctgccaaagg acctacaaag aaacacgtgg aaattttaga agaactcttc
    10861 ccaggctacg aaaacacgtg gcgttcttcc caagagcctg cccgtaaagg ctatgctgga
    10921 accatgtttc tttataagaa agaacttaca cctactatca gcttcccaga aatcggtgcc
    10981 ccttctacca tggacttgga aggtcgtatc atcactctag aatttgatgc atttttcgta
    11041 acccaagttt acactccaaa cgctggtgac ggtctcaaac gcttggaaga acgccaagtc
    11101 tgggatgcca aatatgctga gtatttggct gaactagaca aagaaaaacc agtccttgcg
    11161 accggtgact acaacgtagc ccacaatgaa atcgaccttg caaatcctgc tagcaaccgc
    11221 cgttcacctg gatttactga cgaggaacgt gctggattta ccaacctttt ggcaactgga
    11281 tttacagaca ccttccgcca tgttcatggc gatgttcctg aacgctacac ttggtgggca
    11341 caacgcagca aaacttctaa aatcaacaat acaggctgga gaatcgacta ctggctcaca
    11401 agtaaccgta tcgctgacaa ggtcactaaa tctgatatga ttgactcagg tgctcgccaa
    11461 gaccatacac cgattgtctt ggaaattgat ttgtaaggat tttctatatg gactacaatg
    11521 cggtcattcc cgagttgctt gtatcgaata tcgaacagtc ccggtccttc tactgtggtt
    11581 tactgggttt taggattgaa taccaacgtc cagaagaaaa ctttctcttt ctcttgaaga
    11641 gtgtcaacta atgttagagg agggaacaaa ggatcaacta gctgaactga cctatccatt
    11701 tggtcgtgga gtcaatctct cctttggcat aaaggatgtt tcaaaactct atcaaagagt
    11761 gatgaaggca aactatccta tttatcgtcc cttgaccaaa agaaagtttc gtgtcagtga
    11821 tccctatatc tatcctcatg aatttgcagt tttggatcca gatggctatt ttttaagatt
    11881 tagcgagtag aataataaaa attgggaaaa gtctttaaag ttagaaaaag gcatattgtc
    11941 aggtgtttaa aaacttgata gtatgccttt tatgatggaa aaatcttcta atactcttcg
    12001 aaaatctctt caagccacat caacgtcacc ttaccatagg tatatgatac tgactctgtc
    12061 aattctatcc acaacctcaa aacagtgttt tgaggttgtg gatagcttcc tagtttgctc
    12121 tttgattttc atttagtata aatgttagct tacaatggag tttttgttca ccccctcttc
    12181 tgtttgttcg ttttttaaat caaaacacta acaaaatagt tgatttataa agttatttag
    12241 tgtaaaataa aaacattggc tcaagcctgt ttaaaattga atatcgtttt acttgtttat
    12301 gtcgtgaatt ggcacgacgt ttctacaagg tgccggaaca cctaacaata agtaagtcag
    12361 cagtgagata tggccgtttt tgccatgcct atttggggga cttacttaga gattaagtag
    12421 gtcttttttg aatattttta atatttccct taattagtgc ggacgggagg tgaaattatc
    12481 ctgtgaataa tttcaggaat ccagggaatt tcatccctaa tttttgagcc cctagctctt
    12541 tcatttctag gcttaggcta aaatagttcc ccgaactatt ttactctcaa aaatcccgtc
    12601 atagacaata acaagattat tgtctatgat accactacaa cgggaaatat ttgataagac
    12661 tcgcttcgct cgtaatagtt ttatcaaata tatagtatta gcaaaaggga tcactataga
    12721 aatatttaaa caacaagtaa attaactttt agactttagg aggtcttatg aaattattag
    12781 aagagcgcat cctcaaggat gggcatatct tgggtgataa catcctcaag gtagattcct
    12841 ttttaaccca ccaagttgac tttagcttga tgcgagagat tggtagggtt tttgcggaaa
    12901 aatttgctgc tactggcatt accaaggtcg taaccattga agcgtcgggt attgccccag
    12961 ccgtttttac agctgaagcc ttaaacgttc ccatgatttt cgccaaaaaa gctaagaaca
    13021 tcaccatgaa cgaaggcatc ttaactgctc aagtctactc ctttaccaaa caggtgacca
    13081 gcaccgtttc tatcgctgga aaattcctct caccagagga caaggttttg attatcgacg
    13141 atttccttgc taatggccaa gctgctaaag gcttgattca aatcatcgaa caggccggtg
    13201 ccacagtcca agctatcggt atcgtgattg agaaatcctt ccaagatggc cgtgatttgc
    13261 ttgaaaaagc aggctaccct gtcctatcac ttgctcgttt ggatcgtttt gaaaatggtc
    13321 aggtcgtatt taaggaggca gatctctaat gcaaactcaa gaaaaacact cgcaagcagc
    13381 cgttcttggc ttgcagcact tactagccat gtactcagga tctatcctgg ttcccatcat
    13441 gattgcgaca gcccttggct attcagctga gcagttgacc tacctgattt ctacagatat
    13501 cttcatgtgt ggggtggcaa ccttcctcca actccaactc aacaaatact ttgggattgg
    13561 actcccagtc gttcttggag ttgcattcca gtcggtcgct cccttgatta tgattgggca
    13621 aagccatggt agtggcgcta tgtttggtgc ccttatcgca tctgggattt acgtggttct
    13681 tgtttcaggc atcttctcaa aagtagccaa tctcttccca tctatcgtaa caggatctgt
    13741 tattaccacg attggtttaa ccttgatccc tgtcgctatt ggaaatatgg gaaataacgt
    13801 tccagagcca actggtcaaa gtctcttgct tgcagctatt actgttctga ttatcctctt
    13861 gatcaacatc tttaccaaag gatttatcaa gtctatctct attttgattg gtctggttgt
    13921 tggaactgcc attgctgcta ctatgggctt ggtggacttc tctcctgttg cggtagctcc
    13981 acttgtccat gtcccaactc cactctactt tgggatgcca acctttgaaa tctcatctat
    14041 tgtcatgatg tgtattatcg caacggtgtc tatggttgag tcaactggtg tttatctagc
    14101 cttgtctgat atcacaaaag atccaatcga cagcacgcgc cttcgcaacg gttaccgcgc
    14161 agaaggtttg gccgtacttc tcggaggaat ctttaacacc ttcccttaca ccggattttc
    14221 acaaaacgtt ggtttggtta aattgtcagg catcaaaaaa cgcctgccaa tctactacgc
    14281 agctggtttc ctggttctcc ttggactgct tcctaagttt ggtgcccttg cccaaatcat
    14341 tccaagctcc gtcctcggcg gtgccatgct ggtgatgttt ggttttgtat ctattcaagg
    14401 gatgcaaatc ctcgcccgag ttgactttgc taacaatgaa cacaacttcc ttatcgcagc
    14461 tgtttcaatc gctgcaggtg tcggtctcaa caacagtaat ctctttgtca gcatgccgac
    14521 agccttccaa atgttcttct caaacggaat cgtcgtagcc agcctactcg ccattgtcct
    14581 caatgccgta ttaaatcata aaaagaaata agaaaaagag gtgcgagcct cttttttgtt
    14641 tatcacaaat tgattataaa tacaatcaat cccaattccc atctaattga attggaccta
    14701 tttcaaattc tttaaaatat tatgttccaa atcgttaata ttatataaga atggtaaaat
    14761 atcaaatgtt tctcgtaaat tattcttagc cttataccaa ccttgtccat cagtaatcca
    14821 cataaactca acattaggaa tagctttaat ttcttcagca atcattttgt aagatctagc
    14881 cgtctcattt aattttgaac cacttcctga ataaaaatta acctcaatca gataaagcac
    14941 ttgttcattt tttatgacaa aatcaaaacg tttcacagta ttcccatcat tcgtaatagc
    15001 agataaatcg acagaaaaaa tttcctctat ttcattttgt tcaatctctt taaataaatt
    15061 ctctcctaaa atataccctt cagcttcaag atagctttga acaatatttt ccatcgcatc
    15121 tccagtccga tttttgcgcc cattagtatc cataccaacc tcaacacctg taacgtagtc
    15181 aactaaattt gatactaaat gattttgcaa taaatcaaat atacctgatt tttccaaaaa
    15241 catagtatat tcttcaatac tatagtttcg tttactaaaa tcaaaataga aatcttttat
    15301 cggatcctta acaataatcg tatctctcaa acgtttagca ataagtagag gcacaacttt
    15361 caaaatttct ggataatttt gatataagtc taaaaaatcc tcttgtatat ttttactacc
    15421 aataagacta ttcataatat ttagagcaac ttttatacta gatacatttt tataaacctt
    15481 aggaaaatct gtataataag tccagtcagc tactgtgtcg gtcatcgttg acaaccactc
    15541 atcaaaattt ctagtttgtt tcattttctg cctccaacct cttccttgca atttttaaat
    15601 attctttttc agcatcgata cctataaatc ttctacctaa ccgcttcgca acaacacccg
    15661 tagtgccact accaacaaat gggtctagaa tatagtcacc ctctttagta gaggctaaaa
    15721 taatgcgttc taacaaatac tctggttttt gagttggatg tttcccagcc catttttcaa
    15781 ctttctttgt taaagaaccg gtccagacat ctttcatttg ttttccatca ttcaattctt
    15841 tcattaaatc ataattgtag taatgacgag cttttttatc attttttctg gcccataaaa
    15901 tggtttcagt agaatgggta aaataacgac aagataaatt gggggcaggg tttgttttct
    15961 gccaagtaat attattcaga attttaaaac cttcttgttc taatgccatt ccaactgagt
    16021 atatgttgtg caaactacct gaaatccata ccgtcccatt aggcttcaga acttcttttg
    16081 ctaggcgaat ccatttacga ttaaactcat gtttttcttc gaatgaagaa attttatccc
    16141 aatctccttt atcaacagaa acaacttgtc ccccagaatt agatattcca ccattactta
    16201 aaaaataagg tggatcagca aaaatcatat ccatactttc tggtttcatt tttgataaga
    16261 acttgaacgt atctgaatga acaagaatca tcttattttt attgtagtat ggttttgtca
    16321 taagaacacc tccatactta tattcgttat ttttcataat ttgtgactat aatttcagaa
    16381 atttttcctc gacttgaaga ttttgctcca ttagttcggg tagcttcaac ataatgtata
    16441 ttaaaatcct tatacaactc ctctactaaa gcactagaag aatttgataa cataacataa
    16501 gctcctgtat cactcaatct cttaaaggca tctcttaatc ttacttgatc tgcaaaagag
    16561 aatccctcat gagtataaga cgtaaatgca ctcgtctcag acaatggaat atatggaggg
    16621 tcaaaataca caaaatctcc tgttcgaaca tctacaattg ccttttcaaa atctcccact
    16681 ttaatttcta gttgattgtt atttatataa actgaaatag cagatatcaa ttcctcatca
    16741 acaattttag gattcttata acgtccatat ggaacattaa attgattctt agaattcaca
    16801 cgatatagac cattaaagtt cactctcaac atatatagaa tacgtgcagc tctttgtact
    16861 tcggacatca tatctattct ttcatcacga tctgcagaac gtaaatctaa ataatattct
    16921 tttgaattat attcctgatg aactttcaaa atttcaatca attcttgagg attgtcctta
    16981 atttgttgat agcaatttat tagttcagcg ttaaaatcat taataactgc atctttagga
    17041 gccaaatcaa aaaataaagc tccacctcca acaaaaggtt cgaaatacct gttataggtt
    17101 ttaggcatta attctctaat aacaggcaat aattgtcttt taccacctgt ccatttcgtg
    17161 aacggttgta aagtaacttt ctttatttct tttatcttca tgcttttaat tataccatag
    17221 aaattaaaac acaactcttg tattttctac cacattacta aattttctat tttagaatta
    17281 aatcatatca atatcaaaaa atgagtaagg caaatcttat aaattccaaa attcacctca
    17341 ctctattaaa ttactcttta tatccttacc tgtctttctt ctgaccgctg gcctgtgaca
    17401 aacatggtaa aggttgcttt acagacattt ctgccctctt gattggtgat atccacatcc
    17461 atcacacagg ttgtacgacc ttgatggaca cattctcctt taatggtcaa tacatcgtcg
    17521 agttttcctg ccttaaggta gttgatagag gattggagtg tcaccccatc aagtcccagc
    17581 gagataacca ccaaaccact gatttggtca catagcgtga atagatagcc accatgggca
    17641 ttgccatagt agttgagcga cgagttcact actttggtcg tcaccacaac gtgaccatct
    17701 ctcatttttt caatttcgta attttcaaag gcagatatag cgtcaaaatg aaagtctttc
    17761 atcgtttcct cctgatattt caaacgacac tatgatacta ctttttatag gactgtgcaa
    17821 ggagaaagct cctagtctag taaaattccg acctgttcca caaagcgcat aaaggctgtc
    17881 tgaccttgtt ctgtctgctt gtagactcct gcatcctcaa gtacacgcac aaagatagca
    17941 cccacagagt ccttgacgat tgcaagggct ttttctttat ccgttaggtc tggatgttgg
    18001 gatttgagtt ggtctgccca ctcctgatga taatcggcaa ctgtaacagc ttctcctaca
    18061 agatagctag cgacttgctc cacttcttct ttcagacgtg gtggcaagat tgccaagccc
    18121 atgacctcaa tcaagccgat attttccttc ttgatatgtt ggacatcctt gtggggatga
    18181 tagatgccat caggatgctc tgctgaagtc tgattgtctc gcaagaccaa gtccaactca
    18241 aactgtccat cacgcttacg cgctattggt gtgatggtgt gatgcggtgt cccgtctgtc
    18301 tctgctaaaa tctgcacact agaatctgaa tactgacgcc attcctggaa aatcttatca
    18361 gctaagttaa tcaaatcctc tttggaatcc gaagtcaagc gtaggacaga cattggccac
    18421 ttgataattc cagccttgac ctgctcaaaa ccagcaaatc ggaaggtctt ttgcaaggga
    18481 gccaattcca taggaaatac gtgacggcct ccctgataat gatcatgagt tagaatagag
    18541 ccccccacaa tcggcaggtc ggcattagat ccagcaaaat atcctggaaa ctgctctacg
    18601 atagccaaca gacgttcaaa actctgacga ctaatggcca tgggacgatg ctggccatct
    18661 aaaaagatac aatgctcatt aaagtaagca tagggcgaat actggaaccc ccattcctga
    18721 ccaaccattt caaaacggat aatccggtgg ttgctgcgag ctgggtggtt gacccgacca
    18781 tggtaaccct cattctctaa acaaagttga cactgaggat aattactagc ttgcaccaac
    18841 ttggctaccg caatctcttt cggatccttt tcaggcttag agagattgat ggtaatttca
    18901 agttctccat aatcagatgg aacacgataa gcgatatttt tagcaatggc cttgagtttg
    18961 atgtagtcat ttttccgact gagttgataa aaatcctcta tcgcttgctc aggagagtag
    19021 gcataggctt cccagaagtc acgattgacc tgactcggac aaggagtcac caagtccatc
    19081 agttcagtac caaggatttc acgcgcagtc tgactatcct caatcgtctc taatcgaacg
    19141 gcttcctcaa ctagctggtc cttgaggtca atcaatttat ccagattggt ctcaacttcc
    19201 aaaacacctt ctcccactcg tgccaaaaca cgattggtca ggtagattcg atccatttcc
    19261 tcaaatgaac tttcagaaat gacatgtgtt acaaatttat ctactaaggt cactatagag
    19321 cctccttttg actagtcaag gacgcgagtg ccacctgcaa cttcagcgat atagaagctt
    19381 ggagcgtatc caactacttc ctcgtagtgt ttgcctacag cttccttaaa gacctcaaca
    19441 gtatcttttt gaaccaaggc aatggcacag ccaccaaaac cagcccctgt catacgagca
    19501 ccgagaactc cttcttgtgc ccaagctgtg tgaacaaggg tatctaattc caaaccagtt
    19561 acttcataat catgctccag agaaacgtgt gacgcattca tcaaacgacc aaatgtttcc
    19621 aaatcacctg cttgaagggc tgcttgagct ttaagggtgc gttggttttc aagcacagca
    19681 tggcgagcac gtttcaaacg attttcatct ttaatcagat agctatattg gtcaaaggcc
    19741 cactcgtcca attcacccaa ggtctgaata tccaaggcaa cttgcaactc ttccactgct
    19801 ttttcacact cagcacgacg ttcattgtat ttagagtccg ccaattcacg gcgtttgttg
    19861 gtgttcatga taacaacgac attgtccttc aaatcaagtg gcaccaaatc atactctaag
    19921 gtgttggtat caaggtaaat agcacgttga tcagccccca taccaatagc aaactggtcc
    19981 atgataccag agttgactcc gataaagttg ttttctgttt gttttccgat tttaaccaaa
    20041 tcgagacgct ctaattttaa atcaaagaga tgctctgcca cgactcctgt caagagctcc
    20101 aaggatgctg aagaagacaa accagcacca tttggtatat tcccataaac ataaaaatca
    20161 aaacctttgt caatcacgtg cccagcttct tgcaagaaat gaaggacacc ttttggataa
    20221 ttggtccagt tgtgctcttt ttcaaacttg aggtcagcga gaggcacttc gataatgccc
    20281 ttgtcctcaa agttagctga gtagaaacgc aaaacttggt cgtcacgctt acgagctgca
    20341 ccgtaagttc ccaaggaaat agcagcagga aaaacgtgcc caccgttgta gtctgtgtgt
    20401 tcaccaatca aattgatacg acctggtgaa aagaaagttt ggtctgcttc ttgaccaaaa
    20461 acagcaagaa agtctttgcg aagagtttca gcagtaagat gttgtgtcat atgatttctc
    20521 ctttgactgt ccgttaagcc accttaacgt gtaatcgttt tcttctattg tatccaaggg
    20581 atttcccaag gtcaatcctt tttactaaaa ttttactaaa atatcagtaa aaagcagaaa
    20641 aatatgatat actaacctaa tcaaggagga tttatggcta ccttaaaaga cattgcacag
    20701 ctagcctctg tctctatcgc gaccgtatcc cgtgtcctca accgcgacca gagcctatct
    20761 gttacagaag aaaccagaca ccgtatttta accgttgctg aagagctggg ctacaccaag
    20821 cacctcaaga caggcgagtc ccataaaccc aagcaaaaga ttgccattat ccaatgggtc
    20881 agcgaacaag gggagctgga cgacctctac tactaccaga ttcgcctagg aatagaaaaa
    20941 agagcccaag agttggacta tgatatcttg cgctatttta atgaccatcc ttttacctta
    21001 agcgaggaag tgattgggat tctctgcatt ggaaaattta gccgagctca gatttctgcc
    21061 tttgaagaat accaaaaacc cctggtcttt ctagacagtg atacactttc cctgggacat
    21121 acctgtatta tcacggattt ttacactgct atgaaacagg ttgtcgatta tttcctcagc
    21181 caaggaatgg accgtatcgg gattctaaca ggccttgaag aaacgactga ccaagaagaa
    21241 atcattgagg ataagcggct ggaaaatttc agaaactaca gtcaagcaaa aggaatctat
    21301 catgatgaac tggtctttca aggaaacttt actgcccagt ctggctatga cttgatgaag
    21361 gaagccattc agagcttggg agaccaactc ccaccagcat ttttcgcagc cagcgatagt
    21421 ttagccatcg gtgcccttcg tgcgctccaa gaagctggaa tcagcctacc agaccgtgtc
    21481 agcctcattt cctttaacga caccagcctg accaagcagg tttatcctcc cctctccagc
    21541 atcacagttt atactgaaga aatggggcga gcaggtatgg atattcttaa caaggaagtc
    21601 ctccacggtc gcaaaattcc tagcctgacc atgctgggaa ccagactgac attaagagaa
    21661 agtaccctaa atcaagaata ggataacata aaaaacgaat agagttctaa aactcctatt
    21721 cgttttttat tcgattacaa tcatagactt aattgtctta cgttcatcca tatctttata
    21781 ggcttggtcg atatcttcca gtttataact tgaagtaaag acgcgacctg gattgatatc
    21841 accatcaagg acggctttta gtaaaaattg cttatcgtat gttgtagcag aagctgcccc
    21901 acctgctaca gagatatttt gcataaatgt cgaaccaaga gcacgattat tatagtgtgg
    21961 gactcctaca aagcccatac gccctccatt atgaagaaca cctagcgcct gttctatagc
    22021 agcctccgta ccaacacatt caagtgctgc atctgctcct ccaccgagga tttcacgcac
    22081 cttggtaatt ccttcttgac cacgttctgc aacaacagct gtcgcacctg actccatagc
    22141 catcttttga cggtcttcat gacggctcat aaggataatt tgtgatgctc cacgcatctt
    22201 agccgcgatg acagcacatt gaccaacagc cccatcaccg ataacaacaa ccttgtcccc
    22261 tttttgaaca tttgcaacac gcgccgcatg atagcctgtc ggcatgacat ctgcaagagt
    22321 caaaagggac ttgagcatcc cttctgtata gtcagaaggt tgaccaggga ttttaaccag
    22381 cgcccagttt gcatagtgga agcgaatata ttctgcctga aaatcacccc ccaaattatt
    22441 gccaatatga ttgtcgcaag aaccgtcaaa tccagcaaga caggcatcac actcaccaca
    22501 tccatgtgta aaagggacaa tcacaaaatc acctggtttc accgtcgtaa tggcttcccc
    22561 agcttcttca acaatcccaa tcgcttcgtg tccactattt ttgtgtccag ctttcgtttc
    22621 tggattacgg tacctccata aatctgaacc acaaacgcac gcacgaacca cacgaataat
    22681 cacatcatcc gcttctatta tttgcggacg ttcaatgcta gcaagtccaa cctgacctgc
    22741 ctttgtatat actgctgatt tcatttaaaa ttttccttcc ttataaagtt taattttgag
    22801 atttaaacga tttaaagctg tctgtagctg agacaagcgc tcctctaatt tttgcttttc
    22861 ctcttctaaa ataccaagcc tctcctctct cgtttcatct cccttttggt agagcgacat
    22921 atagtcaact aaactatcta cagagacacc cgccgaacga aaacacttaa taaattccag
    22981 cgcttcgata tcctgatctt gaaaatcacg aatcccagta gcagtacgag taatcggtgg
    23041 cacaagacca acccgttcat aataccgaat cgtatccgct gaaattccca acaaatcact
    23101 ggcagattta atattcacac gctaacctcc tctcatttca ttcactgaat tcacgctatt
    23161 ctaactcact tatcttatta tataactttg agttgactcc aagtcaagca ttttgtatct
    23221 aatcgaaaaa tttttatcat taacaatcat atatctggag tttatacaat gctcttctct
    23281 taccaatttt tgaaatattt aaataataaa gaaattactc tattccaaat aagatagtac
    23341 ccaagtattt ttcacttttt tctaatgttg atgctcataa ctccgttcca agtcacacac
    23401 ctttcgctta tgggtttggt gagtttctag gtcagcatca atttcaatgg taatattttg
    23461 aaaaccacaa tcttttagga aaattcgaat agactcttta caagtttcca tatgttccat
    23521 ttcttttaga caaacatgga caatggcatt tttttccaaa gcatccatag tccagagatt
    23581 aagctgatta aggctggcca cattgtccaa tcgttccagg ccactcttta cttgcttgat
    23641 atcaagacct tctggcacag catccaagaa aatcttgagt gtagaccaaa aacgtgaaag
    23701 ggcttttgaa agaataaaga aagaaatgac aagggacaaa agaggatcta ggatatacca
    23761 gtccgtaaat cgaagaacaa tcgccatcag gataacagct acccacccta gcgtatcttc
    23821 cagaaaatgc agactcagaa tagactcatt ctttgtcttt cccttaccaa ccaccagact
    23881 cgctaacaga ttgatagtaa tcgcaataat tcctaaccag agaatcccct catcattgac
    23941 tggttgcgga tgcaaaatct tcgtgacatt ttccaaaatg actagaacag agcctgttac
    24001 gagaatcaca gctgttacca aggctcctag caggctaaac cgcttatagc ccaaggtgta
    24061 ctgattgtct tcttcacgat tggagattgt ttctagaaaa gctgatattc caattgcaat
    24121 cacatctccc aagtcatgca cagagtcagc aagaacagcg ctagaaccaa atactccacc
    24181 tgcaataaac tcaacaatgg cataagtcaa atttaagaaa aaagccaccc aaacagcata
    24241 ttttgccttc atatttctca ttcctttgtt ataataggtt tatgaacacc ttgttcatta
    24301 tcattatcca ctaaaactca aagaaaggat agaagcaaaa cgtcagcttt attcagttct
    24361 gaacaatttg ccttaagtgt ccatatgact aacattgacc gccgtatcag caaaaccaaa
    24421 aaagccatct atcaagcttt tatacaactt ttgaatgcta agggctacga ggccactact
    24481 gttcaggata tcattgatct cgcagatgtg ggaagatcca ccttttactg tcactatgaa
    24541 agtaaggagc ttcttctgga ccagctctgc cgctacctct tccatcatct ttttgaaaga
    24601 gagcaagcca tttcaaccga ggactacctc gcccacctct ttctccattt tcataaaaac
    24661 caagaccata tcaccagtct gctattttcc aaaaatgact acttcctccg tcaactccat
    24721 aaagagctag aacaccatgt ctattccgtg ctagctgata atttaaaaga agcccaccct
    24781 aatatgccta cttcttacct ccaacacttg gtcgtgtcca actttatcga gacattgacc
    24841 tggtggctca aaaaaggtca agatttcaca gaccaggaag ttgtccaatt ttatctagac
    24901 cttctcattc ctaaaaattg aatatagagt aaagctcagt tgtcttattt ctaggttact
    24961 gagtttttta tcttttcaac aacaaaagag gacccgccga tcctcttttt catactataa
    25021 atccttgatt atcaactata tctgttttaa tcgaaatctc aaaacagcac tttcaaacat
    25081 cttttcctag ttaggtaaat cagtattctg cttagctgcc ttgctccatt gataccaacc
    25141 aactagactg ttaatgagat aaattagata tttcccttga atttgcaggc tttctcccca
    25201 ccagagatag attgaaaaaa cattggtagc cgcccagaat atccactgtt cacggtaaac
    25261 agctgtcatg aggatttgcc ctaccccatt ggttgcatct gtgattgaat cacgataggg
    25321 acgattggca ccaatagact gataaatgaa gccaaaggcc aaccaccaaa gcacactaat
    25381 ggaaagatac tttgtccagc ccttgccgtc cagtttacgc gcgacaaact cctgcttttc
    25441 cttcttaaac tgtgcctgat aaatccaaac tagaagtcca attggctgca tgactgtgaa
    25501 gtaaagtgtc gtcagcacct caccataaaa gcctttctgt agggccaaaa taaggtaaat
    25561 aacagagtta atcaagccaa aaagataatt acttgctcga ccttccgata caaagattac
    25621 acagataatc cctgtcaagc tacaaatcat cccaatccag tcaacaatac gatgttcgta
    25681 aaccaactcc agccagagag gaaaacttcc taaaaccagc aaataaatcc actgggcaaa
    25741 actacgatgg gcaaagaggt catcccagat agccttcata gttcctgaaa atcctaaatc
    25801 agccatagcc gcaaccatac gacggtaacc acctgacatt tcacctaggg ttgttttgat
    25861 attttcaatt ttcttttgca aataagtatg catcatttct ccttttgttt ttaaagagcc
    25921 gtgtctggat agactttcgg acgcaacgct ctattagata atgaactgcc tatacacaag
    25981 atttctaacc ttagtcgaca tgagctgaaa cctcttattt gttaagtagt tcacaaaata
    26041 ttatacacct attttatgaa tagtcaactg tctttacagt aaaattttag aaaatcatga
    26101 aaattttctc tttctttcca ttttaagtga cattcagtca ttctcacatc aaaaaagccc
    26161 agacgaaact gtctgagcat tcttttatct agtcgtttaa ggaagttgag ttcagtatg
//
LOCUS       DAVBDG010000028        24979 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBDG010000028 DAVBDG010000000
VERSION     DAVBDG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24979
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.028"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..614)
                     /locus_tag="VQ526_001119"
     CDS             complement(<1..614)
                     /locus_tag="VQ526_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2554850.1"
                     /translation="MKRITANQYQTSERYYKLPKILFEDEKYMDMKLEVKVAYSILKD
                     RLELSLSRGWIDEEGAVYLVFSNSKLMRLLGCSKSKLLSIKKTLKEYDLIDEVQQSSS
                     EKGRLANKIYLGELSSTPVASPNRPSVKKRLGLYENETAPVSYSAPSETEVSETKFSE
                     TESLFIEDEEDRNSQPILRRKVEKVTKYDRDYIWGLVQDQFRREG"
     gene            complement(611..784)
                     /locus_tag="VQ526_001120"
     CDS             complement(611..784)
                     /locus_tag="VQ526_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554851.1"
                     /translation="MTKELQSSRYIVISFLVREMGIDIVEAISLMAELEKSGLVRLES
                     SGDLILKELGGAL"
     gene            complement(992..2353)
                     /gene="rlmD"
                     /locus_tag="VQ526_001121"
     CDS             complement(992..2353)
                     /gene="rlmD"
                     /locus_tag="VQ526_001121"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2554852.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVECVVLLQR
                     TRG"
     gene            complement(2427..3401)
                     /locus_tag="VQ526_001122"
     CDS             complement(2427..3401)
                     /locus_tag="VQ526_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2554853.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3475..4089)
                     /gene="pmp23"
                     /locus_tag="VQ526_001123"
     CDS             complement(3475..4089)
                     /gene="pmp23"
                     /locus_tag="VQ526_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2554854.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4089..5063)
                     /locus_tag="VQ526_001124"
     CDS             complement(4089..5063)
                     /locus_tag="VQ526_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2554855.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5071..6327)
                     /locus_tag="VQ526_001125"
     CDS             complement(5071..6327)
                     /locus_tag="VQ526_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2554856.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFLSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEDLAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6389..6820)
                     /locus_tag="VQ526_001126"
     CDS             complement(6389..6820)
                     /locus_tag="VQ526_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2554857.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6822..7421)
                     /locus_tag="VQ526_001127"
     CDS             complement(6822..7421)
                     /locus_tag="VQ526_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2554858.1"
                     /translation="MDRIRQELENGGAVILPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7408..8247)
                     /gene="prmC"
                     /locus_tag="VQ526_001128"
     CDS             complement(7408..8247)
                     /gene="prmC"
                     /locus_tag="VQ526_001128"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2554859.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(8247..9326)
                     /gene="prfA"
                     /locus_tag="VQ526_001129"
     CDS             complement(8247..9326)
                     /gene="prfA"
                     /locus_tag="VQ526_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2554860.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9336..9815)
                     /locus_tag="VQ526_001130"
     CDS             complement(9336..9815)
                     /locus_tag="VQ526_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2554861.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVATLAVYDNDAIIGFVMIAYDKKIGISSGNYLLFRFMIDRNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEIAENEVVAIYDLTIEK"
     gene            complement(9805..10404)
                     /locus_tag="VQ526_001131"
     CDS             complement(9805..10404)
                     /locus_tag="VQ526_001131"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2554862.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFTPMITSNKEQNYDN"
     gene            10527..10709
                     /locus_tag="VQ526_001132"
     CDS             10527..10709
                     /locus_tag="VQ526_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2554863.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10846..12219
                     /gene="mnmE"
                     /locus_tag="VQ526_001133"
     CDS             10846..12219
                     /gene="mnmE"
                     /locus_tag="VQ526_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2554864.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12266..13201)
                     /gene="dapA"
                     /locus_tag="VQ526_001134"
     CDS             complement(12266..13201)
                     /gene="dapA"
                     /locus_tag="VQ526_001134"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2554865.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13257..14333)
                     /locus_tag="VQ526_001135"
     CDS             complement(13257..14333)
                     /locus_tag="VQ526_001135"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2554866.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14675..14747)
                     /locus_tag="VQ526_001136"
     tRNA            complement(14675..14747)
                     /locus_tag="VQ526_001136"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14712..14714),aa:Thr,seq:ggt)
     gene            complement(14847..15356)
                     /locus_tag="VQ526_001137"
     CDS             complement(14847..15356)
                     /locus_tag="VQ526_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2554867.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15354..15450)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15479..15910
                     /locus_tag="VQ526_001138"
     CDS             15479..15910
                     /locus_tag="VQ526_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2554868.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            15992..16369
                     /gene="mscL"
                     /locus_tag="VQ526_001139"
     CDS             15992..16369
                     /gene="mscL"
                     /locus_tag="VQ526_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2554869.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16411..17505)
                     /gene="hemH"
                     /locus_tag="VQ526_001140"
     CDS             complement(16411..17505)
                     /gene="hemH"
                     /locus_tag="VQ526_001140"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2554870.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVTLVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRSPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17660..18883
                     /gene="pepT"
                     /locus_tag="VQ526_001141"
     CDS             17660..18883
                     /gene="pepT"
                     /locus_tag="VQ526_001141"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2554871.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18919..19548)
                     /locus_tag="VQ526_001142"
     CDS             complement(18919..19548)
                     /locus_tag="VQ526_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554872.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            19513..19734
                     /locus_tag="VQ526_001143"
     CDS             19513..19734
                     /locus_tag="VQ526_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554873.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(20005..21016)
                     /locus_tag="VQ526_001144"
                     /pseudo
     CDS             complement(20005..21016)
                     /locus_tag="VQ526_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(21242..24361)
                     /gene="phtE"
                     /locus_tag="VQ526_001145"
     CDS             complement(21242..24361)
                     /gene="phtE"
                     /locus_tag="VQ526_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2554874.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKKLSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPGN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLPETGNSTS
                     DSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSETGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(24572..>24979)
                     /locus_tag="VQ526_001146"
     CDS             complement(24572..>24979)
                     /locus_tag="VQ526_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2554875.1"
                     /translation="KPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADNLYKPS
                     TDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAMETLTGLK
                     SSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
BASE COUNT         7155 a         5206 c         4552 g         8066 t
ORIGIN      
        1 ccctctcgtc taaattgatc ttgcaccaat ccccaaatat aatctctatc atattttgtg
       61 accttttcta cttttcttct caaaataggt tgagaattcc tatcctcctc atcctcaata
      121 aataaagatt cagtctcact aaatttagtc tcactaactt cagtctcact aggggctgaa
      181 taagagacgg gggccgtttc attttcatac aggcctagtc tttttttaac actaggcctg
      241 ttcggactag ctactggggt agaagacaac tcccctaaat aaatcttatt agctagcctc
      301 cctttctcac ttgaagactg ttggacttca tcaattaggt catattcttt aagagttttt
      361 ttgatggaca gtaattttga cttcgaacaa cctaacagcc tcatcagttt agaattagaa
      421 aatactaaat aaaccgcccc ttcttcatct atccaaccac gactaagaga taattctaaa
      481 cgatctttta aaatagaata agccaccttt acttctagtt tcatatccat atatttctca
      541 tcctcaaaaa gaattttagg taatttataa taccgttctg aagtttggta ttgatttgcg
      601 gtaattcgtt tcataacgct cctccaagtt ctttgagtat taaatctcca cttgactcca
      661 atcgaaccaa gccacttttt tctaattcag ccataagaga gatggcttca acaatgtcaa
      721 ttcccatttc acgtactaaa aatgaaatga caatatagcg tgatgattgt aattcttttg
      781 tcatttttcc tcctgataat aaaaaaagag aacaatatac aattgttctc tctttagata
      841 aatatttatt gttgtattca tttttccgat acgcaaacga catctacaaa tacatataca
      901 tctatgtgcc tttttagaac ttttttgacg tcaaaattgc ctttatctac aaatacaaaa
      961 tgcttgtaaa atagcttaaa atcaaggatt ttcaccctct tgttcgttgt agcaacacta
     1021 cacactccac gtgatgcgtt tgaggaaata gatccaccgg ctggactttc ttcaattcat
     1081 atcccaattc ttggtagagt ttgatatcac gcgccatggt tgcgacattg caggagatat
     1141 aggcgatgcg atcggctcct gtttgggcgc ttgctttgat aaagctttct gtcaagccct
     1201 tgcgtggagg atcaaccaag ataacggttg gttgaatccc atctttaagc caattcttca
     1261 tagcattttc agctgtgtca cagacatagt gggcgtttga aatattgttc agctgagcat
     1321 tttttttact attctcaacc gcttctggaa tcagttcaac accgtagact tctttgacat
     1381 gcttggcgac tgataaacca atggttccaa taccagaata agcatcaata accacgtcat
     1441 ctttttttaa ctctgcaaag tcaatggctg tttgatagag tttctccgcc atttcagtat
     1501 tgacttggta aaaggctggg ccagcgattt ggaagtcatt tcccaacatc tggtccgtaa
     1561 tatagtcttg accataaaga gtgcgccact ccttaccaaa aatcgcattg gtattctggt
     1621 cgttgatatt ttgcatgaca gacacaatct ctgggaactg cttgataact tgttcaatca
     1681 attggtcaac acgaaaaact tttggacgag ttgtcaccaa aacgaccatg atttgtcctg
     1741 aatagtgacc acgacgcacc acaagattcc gaatcaatcc agactgttcc ttttcgtcat
     1801 aaggttttaa atcaaaacga cggagcaggt ctcgaagagc tactacgact tggtcaatga
     1861 caggatcctg gataaagaaa tcttcaaggg gcatgaggtc gtgcgaattc ttacggaaaa
     1921 atcctgtttc caagacacca ttcactcgac gaacgggcac ctgcgcctta ttgcgatact
     1981 tgactggatg ttccatacca agcgtttcag caacttctac atctgcaatt ccagcaatct
     2041 tgtagagact gtccttgact tgcttggttt taaacttgag ctgttctgga taagaaaggt
     2101 gtcctaaatc cgcgattcct gaacgcaggt aagccaaatc tagatcttga ttacggtgtg
     2161 gtgactggac aaggtatttt tcaacttttc caaagccaat ctttttattg accttgagga
     2221 cacgcatgag aattttttca ctcggtaaag cattctctac aaaaaagacc aaaccatcta
     2281 ccttggcaac tcctgcccct tcatgggtca agtcaacaat ttcaacttct acaatatcat
     2341 ttttctttaa cattcttttc tctttctatt ggccgacaaa aaagacggta acgttacggt
     2401 taccatccat tataccatga aatttcttaa gaaccaaaac tcttgaagaa gttgacaagg
     2461 gcttgccaga gggagcttaa gaagttacca ccgtcctcta gcgccttatc cgcatcaaag
     2521 ttaaggttga tatttttaaa actgtcgcca gcttgtgata cgatgctttg tttaaggtca
     2581 tttagggttt tagtgaaatc tgcattgctg aggatatcac tctttgagag attcaaggca
     2641 aaattgatga tgatattgat ctggtttcct gttatgacct gatcaagttt gtaatttttt
     2701 aaggtatctt caacaatctt gcggatatct tcttctgtca gatttccctt gctttcttta
     2761 gctttggcga gtcctgactt gatatcagct agggcaacgt ttaatttatt agcatcatag
     2821 cctgatttgt ccttgttttc agcattgata tctgacaaag cttttagctc ttcttgagcc
     2881 aaatctttat tagcttgtgg caccttggct ccattagcct ctagcgaata gtaaatccct
     2941 gctaaagcac tttctcctgt aactggaata ggggctgcta cagtgatttt ggcatgttcc
     3001 atacccagcg ttactgctgc gtttcggtac atatcctgag tcaccttagt gatattttct
     3061 ggtgtttcaa tcttgacctc aagtggcgat ttgtcaccta gcttttgaat cttggctgat
     3121 gaatacaact gtaagctaga gtcattggcc acattcatga ttttagaata aacatcaggt
     3181 gtcatggtct tgagttcttt ggtatctgtt gaggcattgt agcccagttt tttaagagtt
     3241 tgattttttt ggtcttcaga tagggaggaa cctaggacat attcaggttg gacataggtt
     3301 tcatcgataa ctttttgaac atctgttgct gcatggacgc tattcatagc tgttactgcc
     3361 cacaagaccg cagcactagt cagaaagagt ttctttctca tagggaattt cctcctttac
     3421 ttctttatag taatatatct atcttaaaga aaacttataa caaaaacacc tggtctagcc
     3481 agatgttgaa aagagagtga aacatttgat gatgtaaagg ttaagtcgta cctgtctaga
     3541 ataataataa tttcctccat ttacatagag ttcagcaccg tgaaaaatgg aaatggggtg
     3601 aatataacta taagtctttc cagtcgtatt accaagcaag ggggcaacag tctcacgaga
     3661 gtactgtttg gctagagcca aggtattttc cttgccattt tgggcgataa aatcgatata
     3721 ggcaggtcca aaattatagg cttgaacagc tgtccagata tctacaccct tcttctgcgc
     3781 cagatagaga ttgcctgtca gagtttgaat gccttgccga atgctagagg cattatcatt
     3841 gatggtgttg gtggaaccac ttgcagactc actagactgc ataacatcgc cttcttttcc
     3901 ttttgtttca gtataaatca tagcaagcac aagctcttcg tttgctgggg tgtctttttc
     3961 actcaagatt tctcgcacca tgggttgata ggtcatgact tgtttgacat cttgatgaac
     4021 gcggtaagct ttatagccag caaaaaggaa gactgctagt acaagcactc ttcgaattcg
     4081 tttaaacatt atttactttg gatatcctcg atatttttga ttaagataga gtaggttcca
     4141 ttttcgtttt ggataaactc aacagactcg gcgtcttgat agacgttatt gggaacgatg
     4201 agctcaattc catttgataa ggagagtttt tggttttcaa atttctttaa ttggcgactt
     4261 gcatcaattt catcaaattg aacaggttct ggtacggctt ctttgacttg gtcaataaag
     4321 ctcaaacgag ccgtcagatt gttgtcaaaa aggtcattag ccaatttctc aggtgacaat
     4381 tcattgcttt cttctaggtt gttgaaaatc gctgatttga ccttggattg aaattgaaaa
     4441 tcatctgtgt taaaagattc agcaattctc tgggctgttt tttccagttc cttgatagat
     4501 tttttaggag aaatcttagg agcgacagca agaagattat ctgaaaaata gttcaaaaaa
     4561 gtcccgttgt acttgattcg tttttcaatc aggtgatact tgcgactctg aagattgacc
     4621 accaaggcct cgtcagcacc cgttccaaat ccaggcaggt tattctgagt cagcttgatt
     4681 ggattatcaa cttctcctcc gaggtgggtc aaggtctccc gcagggcaat tcgcaagaaa
     4741 gcgaaatgtt ctacaccttc tttagaaaat tgaacaaaaa tcaagtcatt ggtcttgaga
     4801 ttttcagaaa tgctaaactc ctctttccag agattagcca gcgttactga tgtctccaac
     4861 aaatcgtctg taatatgatt gaagaaggga ttttcttctt cgaaaatccc agtcttggct
     4921 tcatctgaat acacatgttc aattttttta cgtaggtatt cttcgatttt tggagtaata
     4981 ttgagaaact tatctgctaa gaacagctcg gtatcatccg gactgaactg gtgaataatg
     5041 gctttcttaa tataaatgtc cataaaagtt ttattcctcg tataatggga aggcatctgt
     5101 caattctttg actgcacttc tcacttcttc taatacagcc tcattttctg aattcttaag
     5161 ggttttaatg atgagttcag ccactttgcg actttcttct tcaccaaatc cacgtgcagt
     5221 aatagctgct gctccgatac gaatcccact tgtcttgaat ggtgacaagg tttcgtaagg
     5281 gattgaattt ttatttaggg taatattgac ttcatccagc aagttttgag caacttttcc
     5341 gttttctaca accttagtca catcaacaag gaagagatgg ttttcagttc cacctgaaat
     5401 aatacggaaa tcagggtctt gcaagaagac atctgccata gccttgctgt tcttaattac
     5461 attggcagca tattccttga aagctggatc caaaacttct ttgaaggaaa ctgccttagc
     5521 agccacaaca tgctccaaag gaccaccctg aataccaggg aaaatagctg aattgatttt
     5581 cttagctaaa tcctcatcat tggtcaaaat caaaccacca cgaggaccac gaagggtttt
     5641 gtgggtcgtt gttgtagtga tatgagcgta tggcactggg cttgggtgaa gaccagctgc
     5701 aaccaagcca gcgatgtggg ccatatcaac cataagctta gccccaacag catctgcaat
     5761 ttcacggaat tttgaaaagt caataatttg agaataggct gaagcaccag ctacaatcag
     5821 ttttggtttt acttcttggg cttgtttcaa gatagcatca aagtccaaaa gttccgtttc
     5881 aggatccaca ctataggaaa gaaagttgta ggtttgacca gagaagctaa ctggagctcc
     5941 gtgggtcaag tgtccacctg ctgccaaatc cattcccata accgtatcac ctggctcaat
     6001 caaggacatg taagccgcac agttagcttg gcttcctgaa tgtggttgga cattggcaaa
     6061 tttagcaccg aaaatttctt ttgcgcgttc aatagcaaga gtctctacaa cgtctactac
     6121 atcagttcca ccataataac ggcgtcctgg gtaaccctcg gcatatttat ttgtcaagat
     6181 agacccttga gctgccataa cagccttgga aactacgttt tccgaagcaa ttaactcgat
     6241 attattttgt tggcgttctt cttctttggc aatagcattc cagagatcag catcatatgc
     6301 tttaaaatca tctttgtcaa aaatcatagg tcttctcctt tattgtgtga ctagtccatt
     6361 agtttgattt tacaataaga aaatcaaact aaaagatgcg aataaaccgt ttctgcattt
     6421 tatcacaagt atagccaact ttttcataaa atgcatgagc acccagacga tgattggcag
     6481 aatttaagcg gataaaccca taaccacatc ttttggcttc ttgttccaac ccttgtagta
     6541 aacttttacc gataccttga ccttgcgctt gaggtgaaac tgctaaagct aagatattaa
     6601 atcctgcttt ggaatagagt gattcgtaaa cttcagcgtg gacatatcca agtaagacat
     6661 gattagctgc atcctcatag ccaagtagga aatgatggga atcctgagac agtctagcta
     6721 gttggctagc cgtttcctct ggactaaaag tataacccaa agcctcttgg ttgatgtcac
     6781 atatcgcttt cacatctgtt tcttgcaaat ctcttagcat ttcaagcctc ctcaaaagaa
     6841 atctctggca accgagcaag aatatcttct cgtttaattg cgccttggcg taagattttc
     6901 accttgtctc cagacaaatc cacaatagtt gaatcctgtc cagttagaaa agcatcgtct
     6961 tccagaccca gaacctcttg gtcaaaatcc ttcagaattt gttcaaaggt tacaccactt
     7021 gcctgacctg agatattggc agacggccca atcaagggac ctgtctctcg aattaaatcc
     7081 agtgtgatag ggtgactggg catccgaaat ccaatagttg caaggtcaga atttacccaa
     7141 tagggaactc ggtcattggc ttcgagaata atggtcaagg gacctggcaa aaaggtctct
     7201 acaagttttt gtagataagc tggctgattc ttagaaaagt gcaagatatc ctctaaagag
     7261 gcaacattga gattgagcgc cttgtctcta ggacgacgtt tgagctggta aacatggtca
     7321 actgcttttt cgtctaaggc cttggcaaaa agaccataaa ccgtctctgt aggcagaatg
     7381 acagctccac cattttccaa ctcttgtcta atcctgtcca tcatcaacca cgaccatcct
     7441 atcttgacca aattggtcct tgagtgttcg aactcttttt tcaggaagat gtttcctaaa
     7501 aagttcggga acactttgac cttgcttgta tccaatttca aggtaaatct taccaccatc
     7561 cttgagatag tcttttgcat cttccgcaat tctacagtaa atagctaggc catcctcgtc
     7621 tgcaaagaga gctagatgag gctccgaatg caaaacattc aagcctacct ctgactcatc
     7681 ttcacgagag atatagggtg gattggatac aattatatca tatttttcag aaatttctgc
     7741 aaaacaatca gattttttaa aaaatatatt aagattttga tcgctagcat tctctgatgc
     7801 aagctctaag gcctcttgag aaacatctgc tgctgtcact gaccaatctg gtctattttt
     7861 tgctagagcg agagcaatag ctccactacc tgttccaata tccagaactg agagattcgt
     7921 ctcaagattt tcagttagaa taagctccac taactcctct gtttctggac gaggaatcaa
     7981 aacccgttca tccaccttta aatgcattcc ataaaaatct gcctgtccaa tgatgtactg
     8041 agctggcttg tgagctgcta gttgctggta aatatcttct acaaattgtt tttcttcctc
     8101 tgttgtcacc tcctgctgga gggcaaaaat aaagtctgta aaagatagat ttttcagact
     8161 acgatagaca aaagagaggc tttccgcttc ctctccttgt cttatcaact cttcttcaaa
     8221 atttgaaaat aattgagcta atttcattat ttgtttaatt cttctagttt ttgtgtttgg
     8281 tcataaagca ccaaggcatc cacaacttcg tccaatttac cagacaaaat cgtatctagt
     8341 ttttggaggg tcaagccgat acggtggtct gtgacacggt tttgtgggaa gttataagtt
     8401 cggatccgtt ctgaacggtc accagtaccg attgtcgact tacgctcagc gtcttgttca
     8461 tcctgagcaa tctgagcaaa gtggtcagcg acgcgtgcac ggataatctt catagccttc
     8521 tcgcggttct tctgctgggt acgttcttcc tgcatctcaa ccttgatatt ggttggcaag
     8581 tgaacgatac gaacggcagt cgcaacctta ttgacgttct gtccaccagc accagaggcg
     8641 tgatagatgt cgacacgaag gtcttttgga tcaatgtcgt attcaacctc ttcaacttct
     8701 ggcataacaa gaactgtcgc tgtcgaagta tgaacacggc cttggctttc tgtcacagga
     8761 acacgttgca cacggtgggc acctgattca tacttaagct tagagtatac agactgacct
     8821 gaaaccatag caaccacttc tttaaaacca ccgacaccat tcatagaggc ttccatgact
     8881 tcaaagcgcc aaccttgggc ttccgcatac ttttggtaca tagttagcaa atctccagcg
     8941 aaaagtgccg cttcgtctcc accagctgct ccacggattt caaggatgat attcttgtca
     9001 tcgtttggat cctttggaag gagcaaaatt ttcagttttt cttcatattc ttctttttca
     9061 gccttggcat ctttgagttc ttgcttggcc atttcttcca agtccgcatc tccgcctgat
     9121 tccttaatca tctcttcggc atcgacgata ttttgaagga cttgtttata ctcacggtag
     9181 gctattacgg tgtcacgatt ggaagcttct tcttttgaaa gctccataaa acgcttggtg
     9241 tctgaaacga catcagggtc actcagcaat tctcctagtt cttcataacg gtcttctaca
     9301 gcttgtagtt gatcatagat gttcattttt tctccttatt tctcaattgt taaatcatag
     9361 attgctacta cttcattctc ggctatttcc ccagtttctt taaatccata actgaggtaa
     9421 caaaatcttg cctgttcatt ttctggttca taagacaacc aaagtttatt gcttaaacct
     9481 gctggcgctg ttcgaacata gtctagtact ttatccataa ttggtttaaa atatccttga
     9541 ttttgaaaat tcctatcaat cataaaacga aatagtaaat aatttccact actaattccg
     9601 atctttttat cataagctat catcacaaaa cctataattg catcattatc ataaactgcc
     9661 aatgtagcta caaaatctcc atttttagtg tagacgtatg cttcagctaa actaattgcg
     9721 ttggttgcaa tgaattgttt ttgatattcc ttgacatcca aatttaaaac atcaaaataa
     9781 ttttccattg taacatctct tagttcaatt gtcatagttt tgctccttgt tagaggttat
     9841 cattggcgta aaataatgtt tacggcaaac tgagatatag gtttcgttac caccaatctg
     9901 gatctgttct ccatcgtaaa cgggcagtcc atcctgtgtt cgcaacacca tggtcgcctt
     9961 tttcttgcaa tactgacaga tggtcttgat ttcgtcaatc ttgtctgcta aaagcaagag
    10021 atatttggaa ccttcgaaca attcattgcg aaagtcattt ttcaagccaa aagccatgac
    10081 gggtatgtct aactcgtcca caacacgagc taggtcgtaa acatggtggc gtttaagaaa
    10141 ctgggcttca tcgaccaaaa cacagtaagg tttttctggt aggtctcgga tatagccaaa
    10201 gatatccgtt gtttcctcaa tcgcaagggc agggcgtttc atgccaattc gactcgacac
    10261 atagccaaca ccgtcacgcg tatccagagc cgaggtcata atgacaacac cttttccttg
    10321 ctcctcgtag ttataggcca ctttgagaat ctcaatcgtt ttaccagagt tcatggtccc
    10381 ataacgatag tacaactgtg ccatgtttct tgcttcacgt ccatttctaa atttttgcta
    10441 cattctagta tatcataatt ttcttaagct ttaaacggca aaatgtggta aaatggaaga
    10501 aatcaaaact agtggaggaa gctattatgc catttgtacg catcgattta tttgaaggac
    10561 gcacgctcga gcaaaagaaa gctcttgcta aggaagtaac ggaagcagtt gtccgcaaca
    10621 ctggagcccc tcaatctgct gtccatgtca tcatcaacga catgccagaa ggaacttact
    10681 tcccacaagg ggaaatgcgt actaaataag ctagcttaag cagaattgct taggcttttt
    10741 caatctccaa gtagcattca ttgaagaaat atcctaaatt tgttacaatt tgaaaagaaa
    10801 cttggagaat ttccaagaaa agagctatta attaaaggaa acattatgat tacacgtgaa
    10861 tttgatacca tcgctgctat ctctactcca ctaggtgaag gggctattgg tattgtccgc
    10921 ctgagcggaa cagacagttt tgctattgcg caaaagattt ttaaaggaaa agacttgaac
    10981 aaggttgcca gccacactct caactacggt cacattattg atcctctgac tggtaaagtc
    11041 atggacgagg ttatggttgg ggctatgaag tctccaaaga ccttcactcg tgaggatatt
    11101 atcgagatta acacccacgg tgggattgcg gtgaccaatg aaattctcca gctagctatt
    11161 cgtgaagggg ctcggttggc agaacctggt gaatttacca aacgtgcttt tttaaacggt
    11221 cgcgtagact tgacacaggc agaggctgtg atggatatca tccgtgccaa gactgacaag
    11281 gccatgaaca ttgcggtcaa acaattagac ggctcccttt ctgacctcat taacaatacc
    11341 cgtcaagaaa tcctcaatac acttgcccaa gttgaggtca atatcgacta tcctgaatat
    11401 gatgatgttg aggaagctac tactgccgtt gtccgtgaga agactatgga gtttgagcaa
    11461 ttgctaacca atcttcttag aacagcccgt cgcggcaaaa tccttcgtga aggaatttca
    11521 acagctatca tcggacgtcc caacgttggg aaatccagcc ttctcaacaa cctcttgcgt
    11581 gaggacaagg ctatcgtaac agatatcgct gggacaacac gagatgtcat cgaagagtac
    11641 gtcaacatca atggtgtacc tctcaaattg attgatacag ccggtattcg tgaaacagat
    11701 gacatagtgg aacaaatcgg tgttgagcgt tcgaaaaaag ccctcaagga agccgacttg
    11761 gttctactag tgctaaatgc cagtgaacca ctgactgcgc aagacagaca acttcttgaa
    11821 attagccaag ataccaatcg cattattcta cttaataaaa ccgacctgcc agaaacgatt
    11881 gaaacttcga aactacctga agacgttatc cgtatttcag tccttaaaaa ccaaaacatc
    11941 gacaagattg aagagcgaat caacaacctc ttctttgaaa atgctggctt ggtcgagcaa
    12001 gatgctactt acttgtcaaa cgcccgtcac atttccctga ttgaaaaagc agttgaaagc
    12061 ctacaagccg ttaatcaagg tcttgagctg gggatgccag ttgatttgct tcaagttgac
    12121 ttgactcgta cttgggaaat cctcggagaa atcactgggg atgctgctcc agatgaactc
    12181 atcacccaac tctttagcca attctgttta ggaaaataag aaaaatccat gatccttcat
    12241 tcggtcatgg attttattgt ctttattagt aatctggtct taagacccct gttacagttg
    12301 ccttagttgc ttcgtagtcg ccatctacga caaccttgat aatgcgtttg gcatcttctt
    12361 ctggtgctgg aacaagaggt agacgagtgg gtccagcttc aaatcccata tagttaagaa
    12421 ttgccttaac tggagcagga cttggataag agaagagagc attaacctta ggaatgaatt
    12481 tacgctgaat tgctgcggct ttcttcatat cgctttctgc aatggcagta aacatctcgt
    12541 gcatttcatc cccatttgta tgagaggcaa cagaaataac cccatccgcc ccaaggttca
    12601 tggcatggaa agcatctcca tcctcacctg tataaatcaa gaactcttca ggcttgtgct
    12661 caatcaagta agccatatta gccaagctag tacattcttt gacaccgata atatttggat
    12721 ggtcagccaa gcgaagcatg gtttctggag tcaattcgac aactacacgc cctggaatgt
    12781 tatagataat aattggtagg tcagaagcat ctgcaatagt cttaaagtgc tgatacatcc
    12841 cttcttgaga aggtttgttg tagtaaggaa caatagcaag cccagctgcg aaaccaccaa
    12901 attccgctac ttctttgaca aactcaatag agtcacgcgt atcattagta cctacacccg
    12961 caatcaaagg aacgcgtcca ttgacaacct tttgtacagc cgcaaacaac tccaactcct
    13021 catcgtgggt caaagttgga ctctcagcag tcgttcctgc gagaagaatt ccatccgtat
    13081 gatgggccaa taaatgctca atcaaggctg gaatagcatc aaagttaatg gaaccatcct
    13141 catggaaggg ggtaataaag gctgtaatga ttttacattc ttttaaatct tgataagaca
    13201 cgaaaacacc tctctatttc aaagaaggag ttcatctgaa ctcctaaacg atatgactat
    13261 tttaattcaa atttcaattc ggctgttgga cgaaccaatc cacgttcatg aagagtttca
    13321 gcaatctgaa ctgagttcca agcagcacct ttgagaaggt tatctgaaac aacccacatg
    13381 tgaattcctt tttctgcatc caagtcttta cggatacgac caacaaaggt atcacgcgaa
    13441 ccaactgcat tgatagcttg aggatagatt tgatgagcta catcatcttc aagaacagca
    13501 cctgggaagg ctgcgatagc tgcttttact tcttcgattg gagccacttc ttttgtttcg
    13561 atataaacag actcagagtg agctgacaag actggaatac gcacacatgt tgcagatact
    13621 gcaatgctat catcttccat aattttctta gtttccttgg tcatcttcat ctcttcgtac
    13681 gtgtaatcat tatcagtgaa aacatcaatt tgtggaagag cgttaaaggc gataggataa
    13741 tgtttcttgt caccacctga aggcaagatt tccgcatgca aatcacgtgg tttcacacca
    13801 tcattcaaaa cctcacgaag ttcacgttgt gtctcaagaa ttgctcccat accagcacct
    13861 gaaacggctt gataagttga aacaatgata cggtccaagc cccatttttg gcgaaccggc
    13921 tcaagagcca ccatcatttg gattgttgaa caattagggc aggcaatgat tccgttgtga
    13981 gcatcaagtg catgagcatt gacctctgga acaaccaaag gaacatctgg attttgacgg
    14041 aaataagatg tattatctac tactaccacg ccagctttta ctgcgtatgg tgcatactta
    14101 gctgatgtag aactacctgc tgaaaagaga gcaatatcaa ctccttcaaa agctgtttca
    14161 gtcgtttctt caattgtaat atcttgatct ttaaatttca atgacttacc tgctgaacgt
    14221 gcagaagcaa ggtaacgaat tttatcgatt ggaagtgttg attcttccaa catttttatc
    14281 atctgagcac cgacagcacc tgtcgcgccg actacagcaa ctgtatatcc cataaataac
    14341 ctctttcgga attttctaaa aatttctata atagaagtat tatactactt tttccatgaa
    14401 ttgcaaagct ttttcatcat tttttggaaa ataaaaatcc cagtcgctag actagggact
    14461 aagaggcatc ttcatgtgat gagcaggttc acacaactca tcaaggtccg ctcctgcgtt
    14521 atgacctcct tatgctcaat agtagtccga agactaccta tcgactcatc gcatattcta
    14581 tattactaga gatacttgat tttgtcaata agaaattttt tcgatcgagt ataacaaaaa
    14641 actctcgtat tcaagagttt ccctatacat atcatgctaa ttgccgggat tgaaccggcg
    14701 acctcatcct taccatggat gcgctctgcc aactgagcta aatcagctta cttaagaagt
    14761 atactatatt cagaaaaact tgtcaagttt ccttagaaat tttccatagg aaaaagccag
    14821 gtaagaacta cctagcttat cttcttctat ttacttaaat caattcgacg accaatttta
    14881 ccaataataa tcgccccaat aatcaatgaa gcaaattcac cgattcctgt tgtaaaccaa
    14941 gtaaggaaga atggtaattc tgctacaata tttaactctg cagcgatagt aaacatggaa
    15001 attgaaaaga ggattgaaaa gaagaaatga tcttttcgaa tcaatccgtt aaagaggtaa
    15061 tccttgctat attttgcaaa aagccaaaca cctaaactga ggaatactaa ggttgaacca
    15121 ccaccaacaa agacatctag cagtccgaag ctaaagaaat tagcaatcat acaaccgatg
    15181 gtaactccga tgatgtactt aggattgtaa aaagccataa agttcatcat ttctgaaata
    15241 cgaaactgat aagcaccata gctgatggca tttaggggcg gtgtgacagt caaaacgacg
    15301 tagatagcag cgacgattgc aatatctgca acatcacgaa tagttaattt tttcatcttt
    15361 tctcctttgg cggttgcccg cgtgtaatat gcttggtgaa agaagctaag caccaagggt
    15421 tgctttattc aaccctacta gtatagcaca atagcctgct ttatgctata cttaacacat
    15481 gaaaattcct attcaaaaat tttttaacaa tgaaatctta gcctatctct tttttggcct
    15541 tgcgacaact ctagtttcga ttctctcacg actagtcatt tatcaactaa gtcatcagga
    15601 actcctggct actgccctag caaatattat cggtatccta tttgccttta tcacaaatga
    15661 tacgattgta tttaagcaaa aaaagaaaaa tagacttatc cgtttagtga aattttctct
    15721 tgctcgcctt tctacttttc tgttagactt gtttttaact tttctctttg taacccaatt
    15781 tcctcacatt atagggcaat tcgtaactga aaacattgat aagataaatg ctattgaaac
    15841 aataattgca caaatattca ttattatcat caattatatc ttgagcaagg cctatatttt
    15901 tagaaaataa tgctccattt atgagccttt taattgcctt ctataatgat ttaggataca
    15961 atgaaacacg aaacattgaa aggaaaacag tatgttaaaa aatctaaaat cgttcttgct
    16021 tcgaggaaat gttattgacc ttgctgtcgg tgttgtaatt gcctctgctt ttggtgctat
    16081 cgttacttca cttgtaaacg acattatcac tcctcttatt ttaaatcccg ctttgaaagc
    16141 tgctaaagtt gaacgtatcg ctcaactttc ttggcatgga gtcggctatg gtaacttctt
    16201 aagtgctatt atcaatttta tctttgtggg taccgccctc ttctttatta tcaagggcat
    16261 tgaaaaagca cagaaactga ctggcataaa gaaagaaaaa actgccgaaa aaaaaccaac
    16321 cgaattggaa gtccttcaag aaataaaagc tctccttgag aaaaaataat cgattaaaag
    16381 gatctagcgc aaagctaaat ccttttttct ttactctttg ggtaacttgc ctgctttttc
    16441 aagcattttt ttgatcagat aaggcatctt aacattctcc cgcccttttt tctcaatcag
    16501 ttttttcaca aattctggca tttgtaaatc ttcagattca gctaaaatgt ttttcgtctc
    16561 gtctgaagga actaactcat caaaggtctc ttcttctggg agaaattcct taaactcttg
    16621 attttcattg actctaactg tattgaccaa agcatcaatc aaacgagaat ctgtatttgg
    16681 cattggtgaa cgatggtagt ttaccccaaa ttcctggcat aagtcataac attctacatc
    16741 attgtcaaac aagacctcaa tatgctcact gatgaaacta ataggtacaa aaatataatg
    16801 gtctggatgt tctgtctgtt ctctgagata ctccaaaaca tctggtttaa tccatggaat
    16861 accaatatca ctctcactct gccaagtgtt ggtatattgt tctgaactca agcccagttt
    16921 ttcagcgact agcttgctat tttcgaaaat ttggtctata taaggatcac caaagtccaa
    16981 ggcaaaaatg ggcacactat gggctgaaaa gatgacttta aagctgtctt gctttacttc
    17041 ttcttttaaa atcttagcaa tttcatctgc ccaatagttt aagagcactt cttcctgata
    17101 ccagtcctta atgaccaaaa attgaatttg tttgctttct aaaaattttt catagcccat
    17161 gacagagtag aaagaataat ggggctccaa aatcaagcaa atacactgtt caattccgtc
    17221 tgcttccatt tgaccgatca catctggaat aaagggactg gaaaacttat tagcaaagta
    17281 gacactgtat tcattcccca gcctagcttc taccaaggta acctcttgac gggtaatttt
    17341 ttgcagaggc gtgcctccaa ttcgtacata attatcatag agagtctgaa tctcgtggtc
    17401 ttggggtctc accccacgac gaatatttgt gaaaaaatca gctacacctt caaaggtaat
    17461 ctcttctggt gaaccgaagg tcatcattaa aattgctttt ttcatgactg cttccttgtg
    17521 atgtttttat caagtttatt ttatcattaa ttcatcgata agtaaacgct aacataggga
    17581 cattctcaat cttctgtctt ttgtttttct cttctttcta tgatacaatg gaaaaaataa
    17641 attcaaaagg agttttttta tgacttatcc aaatctcttg gaccgcttct taacctacgt
    17701 taaggtcaac acgcgctctg atgaacactc tactactact ccaagtacac agagtcaggt
    17761 tgacttcgca acaaatgtcc taattcctga aatgaaacgt gttggactgc aaaatgttta
    17821 ctatctaccg aatggttttg ctattggaac cttgccagcc aacgatccgt ctttaacacg
    17881 taagattggt tttatatcgc acatggatac tgctgatttt aatgctgaag gagtcaatcc
    17941 acaggtaatt gaaaactacg atggtggtgt gattgaacta gggaattctg gtttcaaact
    18001 cgatccagct gacttcaaga gtcttgaaaa atatccagga caaacgctca tcacaacaga
    18061 tggaacaacc ttgctaggtg ctgatgacaa gtcaggaatt gctgaaatta tgacagccat
    18121 tgaatatcta actgctcatc ctgaaattaa gcactgtgag attcgtgttg gttttggtcc
    18181 agatgaagaa atcggtgttg gtgccaataa atttgatgca gaagattttg atgtggattt
    18241 tgcctacact gttgatggtg gtccactagg tgaacttcag tacgagactt tctcagccgc
    18301 tggtgctgaa ttgcatttcc aaggccgtaa tgtccaccct ggtactgcca aagggcagat
    18361 ggtcaatgcc cttcagctag caattgattt tcataatcaa cttccagaaa atgaccgacc
    18421 tgagttaact gaaggttacc aaggttttta ccatctaatg gatgtgacag gtagtgttga
    18481 ggaggcgcgt gcaagctaca tcattcgtga ttttgaaaaa gatgcctttg aagcgcgtaa
    18541 agcatccatg caatctatcg ctgataagat gaatgcagaa cttgggagct accgtgtcac
    18601 tctcaacttg acagaccagt actacaatat gaaagaagtc attgaaaaag atatgactcc
    18661 aattaccatt gctaaagccg ttatggaaga tctaggtatc acgcctatta tcgaaccaat
    18721 ccggggtgga acagacggct ctaagatttc ctttatggga atcccaactc cgaatatctt
    18781 tgcaggtggc gaaaatatgc acggacgttt tgaatacgtt agccttcaga ctatggaacg
    18841 tgcagttgat accatcattg gcatcgtagc ttataaaggc taaaaagacg aggtagctca
    18901 gctacttcgc ctttcttttt attctactgg tttttcttga tttccagtag ttgtagaaga
    18961 ttctgttgtt tcattttctg aagttgattc agcaggttta gaatctcttg tattgcttgg
    19021 tttgttttcg tcgctagcaa tttcaatgtt agattctgca gttgcgtttg gttggttctc
    19081 agcactggtg ttatcactat ttgcttcagc atttcttgct ggacttgttt cttcacttgc
    19141 gctagctttt gactggattt gatgattcaa aactagaata gcttttgtcg attcaagtaa
    19201 agctgttttg tctttactct tagcagaaag ttgatctaat aatgcatcca ccttatcaaa
    19261 gtccgcatca gatccattat tactttctaa ataagagtga agcgacatga gaatatcgta
    19321 gagtttttga tagagtacaa gtgtctgaga atcttgctca gcattttcct tttcttgttg
    19381 aagggcgcta gcgatacgag tcaagacatc ttttacctga ctgtttactt catccaagtc
    19441 tgcatcagcc ttgtttgtgg cagcttttag attttctact tcttctgcca aggattgtct
    19501 gattccttct tcatggattt gttccaagag ttgatttacc ttgctcaaaa gactttctac
    19561 ttcttccttg ctatctgtcg cagattattg gttgccatct accatgtact cctaaaacag
    19621 gagagttata atccaagatt acaaggcctt acagaaataa gaaatccaga taagacaatg
    19681 ttcgtccaag acgctattcg cttcgcacag cagcacggat tcaatatgct ttaattttaa
    19741 agtttaggtg tcaagacctc tttttagtgt gcccaaaatt tagagaagta atcaatcaac
    19801 taacttttat ttttttcaaa ctttcagtaa actgacctaa agctaactca atctgtcttt
    19861 gtagatgctt ctgctatcag ctagaagttg atctactttt gccaagactg ccttctcatc
    19921 aaaagttcca ggttgatagt tggattgcag ggatggaatc ttgtttttca aagccgcttc
    19981 atatccctta gtttgaacct tgatgtagtg attgtggtcg ccatgaggaa tcacaaaacc
    20041 ttctgaatct tcacttataa ttcgattggc atcaaaacca tgaccatctt cttcctcatg
    20101 atggacatgt agtgacggat tacttaatac agaactagaa gaacttccta cctcttccgt
    20161 gttagagtgt gatgggggat tgttaagaga tgacttagga atatagtgat agtgatcccc
    20221 atgtcttact atataagcat cacctgtatc tctgacaata tcattagggt taaagacata
    20281 tgtggctgct aattcacctg ccgacaagtc actctcagga atgaaatgat agtgaccacc
    20341 atgtggtact atagtagatt gaaatagaat atgagcaaat tgataagggg attttaaagt
    20401 aatttctaac aatgatttag aaactatgat gtgctattct aaattcaact cactatatat
    20461 aaccatcatc ggtagtataa cgtccctgta attttgctac agatacttct gcactagctc
    20521 ctttatcgtc tttaccatgt tcttgttttt ggcgattgat ttcatctttt gttcgtacat
    20581 tttctgcatg agcttgatct ttaaggtaaa cataatactt tccatctacc ttaataatat
    20641 atcctccctt aacctaactg acgatatctt gatctttcgg ctgatagttg ggggctttca
    20701 ttaatagctc ttcactaaag agcgcatcaa aaggaacttt accattatag tagtgataat
    20761 gatcgccatg agaagttaca taaccttgat ctgtaatctt aataacaatt tgttttgctt
    20821 gaattccttc tttttgacta acctagtctg gagtcaaatt ttcagtcttc ttagtgtctt
    20881 tattactgtt tacatatgaa acacgatttt tatctgtatt ggcctgttag ctatgttggt
    20941 tcagagcata aacacacaga cttaaggaaa ggataacaac agatccagct gctatatatt
    21001 tctttttaaa tttcataatt acctcatttc tataattatt tatatgatgt cttcattatt
    21061 aaatgattaa ataaattaat taaccaatta attaactagt aaatattcca cctcttttta
    21121 agttgtatgt caagaaattt tatatattaa taataaaatg aaattctccc aaagtcagag
    21181 ttttatttct aacttttgag agaacttcat ttttgattca gactttttct actgctattc
    21241 cttacgctat gagatcagat aaattctttt ttatcacttc tccacttggc aatcttaatt
    21301 caatcgttcc atccatattg aatataacac tatctaagcc taatccgtaa ctagctgtaa
    21361 atttttctaa tttttcttgt acaggatcta ctgctggagc ttcctctaat gctggatcta
    21421 acatagggtc actccccaca ttcccttctg gattcaacat tccattatcc gttgagtttt
    21481 ctggttttac aggtttttcg tttggtgcct ctggtaaaga atctgctggt ttattttctg
    21541 ttggttggtt ctcaactgtt ccagtagata cttttccagt ttcagatggt ttattttcac
    21601 catttccttg aggtgcttct cctgtaaaat ctgccatatt ctttttaatg acttctcccg
    21661 atggtaaata taattcaatt gttccgtcca tattaaacaa gacattttct agcttcatcc
    21721 cataactttc agcaaatttt gctacttttt cttgtacagg atccactgta ggaacttctt
    21781 ctaacgttga atcactagta ctgttcccag tttcaggaag cttttctttt tctaccttct
    21841 cactagtctt tggttcttct accttttcat caagttttga gttttcttgt gctttattcc
    21901 ttttaaattg tggtagaata cttggtttat cagtttgatt ttctttttcc aagataggta
    21961 cttccacaat ataagtcgat tgattgtcca aataagcatt tgccatgaag gttacaggaa
    22021 ttttatttcc ggccgttctg gttgttcctt ggtttaattt cggaatcggt aatttaattt
    22081 caccaacttt atagttattt tctaaataag catttccatg aaattcatca aacactctga
    22141 ctaaagcatc agttccttta ggcactgcaa attgagggtt cactcttaaa taagtatccc
    22201 ctgcatggaa aggatagaaa atcgtttgac tggccatttt gtaagctaaa gaggttggaa
    22261 ctgtaaatgt accatcataa cttacttctg gataatcttt tgaagcgata gtatacttaa
    22321 atgtttgtcc tggtaaataa ggttgatcta attcaaagtt tgcaatattc cctactcctt
    22381 ctccaaatac tttaccagat actttctcca atacttttcc atctggtgtt attaatttta
    22441 ctagcatatt gatacctaat tttttttcca attcaggcgg aaaactaaaa gaaacgcgtt
    22501 tttgaccatt ggctagagta aagttttgat tattaaacgt actatttttt aacaaattaa
    22561 caacattcgt taattcttct ccagtataaa ctttattccc ttctttttta gcaactcctt
    22621 cttcgggttt aaacagttca tagttactgt gagaatgacc aattccaacc ggtttatgtt
    22681 catcaatcgg atctgcatga tggtgatctc catgcggata aataatcgca tttttttctt
    22741 tattcacgac aatactttca cgtttgacac catattgttt cataatgcca gcaatttttt
    22801 cttcgatttt tttatctaaa tctttcattt ctttggcatt acctggataa tcctgttcat
    22861 gagatgacaa agaatctaat ccattatgac tagttttaac ttcctctaaa tgtttttgcg
    22921 cagccttaat ttgctcttct gtcaagtcct tcttgaagaa ataatgattg tggtctccgt
    22981 gactcatgac aaaacctgat tcatcttcag cgataatacg attagcatca aatccgtatc
    23041 catcttcttc atgtttctca tgtgaagttc ctggattgat tggaagagat ggagaaggtg
    23101 ttgctagact attgtttgga agagtcggtt gcccaatttg atttgatttt ggaatgtaat
    23161 ggaaatgatc accatgtctt acaatataag ctgtagccgt ttcttcaacg atatcttttg
    23221 gattaaaaat ataaccatca gatgctgaag agagtttctt acttgtcgtt aaagaagaag
    23281 gattgcttga aagactgcct agactagaca ctacttcatt aggttttgca tttgtagaaa
    23341 ctgtagaacc agttccactg ataggcacca ttctggcaat cttttcttct aaggcagaaa
    23401 gcttgctgta aggaataaag tggtaatggt cgccatgcgg aatcgcaact ccatttggtg
    23461 tacgactgat aatcttagca gggtcaaaga ccaggccatc tgattcactg taacgttggg
    23521 cgctaggtga atcatagagt tccttcaaaa gactctggag attttcagat ttatttgctg
    23581 gcttgctagt tgatcctttt gctacagatt gcgtgttatt gtcactagct gttgaagaat
    23641 agcttaactg actcggttgc atattttttc cagccagatg tgctttagct gctgctaatt
    23701 cactagcaga taaatcgctt ttgggaatgt agtgatagtg acctccatga ggaacgatat
    23761 aagcattacc cgtatcttcg ataatatcag ctggattaaa gacataacca tcatttgtcg
    23821 tatatcgtcc ctgagacctt gctacagcaa cattagagtt aaccttctca ttatctttga
    23881 catgttcttg tttttgacga ttgatttcat ctttagttcg aacattatca gcatgagctg
    23941 catctttcag gtagacataa tattttccat cgaccttgat gatataacca cccttgactt
    24001 cattgacaat atcagcgtct ttaagttgat agtttggatc cttcatcaag agttcttcac
    24061 taaagagggc atcataagga actttcccat tatagtaatg atagtggtca ccgtgtgacg
    24121 ttacatagcc ctgatctgta attttgatta caatttgctc agcctgaatt ccttctttct
    24181 ggctaacctg gtctggtgtc aagttttcac ttttctgact tgactggctg ccatccacat
    24241 aagagacacg attattgtcc ttattttcct gcgaacgatg ctggtttagt gcataggcac
    24301 atagactcaa ggatacgata acagctgatc cagctgctat atatttttta ctaaatttca
    24361 taaatccctc atttcaataa atgatgaagt ttttttctca acttctttta ctttattaaa
    24421 tagttttcta aaccgattat ttaaccattt aattaaccag taaatagtct actatttaaa
    24481 attacttttg tcaagtttct gttacgtttt tctaaaaaag agaagtaaca cgaaaggtta
    24541 cttctccata aaatatgctc cattcatttt actactgtat aggagccggt tgactttctt
    24601 ttaacaaagc caagagacta tctacttctg ctgaaatagt attattatct ttcgttccga
    24661 gaagaagact actttttaga ccagtcaatg tctccatagc attttgtcta atactagaat
    24721 ctgttacttt ttctagcaag gcctccgcct ctgctatctt agcgttaata acagaatgct
    24781 ctacttgagg aatttcagcc tcatctgtag tatcttcagc ttcttcctct gtctcttccg
    24841 tatcagtgct tggtttataa agattatctg ctgaaggatt taaaccagcg tgattctctt
    24901 tttcatcaga ttcagggtga gttggctcac ttacttcatc atgttcctta tcttcctcag
    24961 gtttttctgt ctgaggttt
//
LOCUS       DAVBDG010000029        24222 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBDG010000029 DAVBDG010000000
VERSION     DAVBDG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.029"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(15..210)
                     /locus_tag="VQ526_001147"
                     /pseudo
     CDS             complement(15..210)
                     /locus_tag="VQ526_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(272..721)
                     /locus_tag="VQ526_001148"
     CDS             complement(272..721)
                     /locus_tag="VQ526_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2554876.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(723..1481)
                     /locus_tag="VQ526_001149"
     CDS             complement(723..1481)
                     /locus_tag="VQ526_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2554877.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1596..3575)
                     /gene="pknB"
                     /locus_tag="VQ526_001150"
     CDS             complement(1596..3575)
                     /gene="pknB"
                     /locus_tag="VQ526_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2554878.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3572..4312)
                     /locus_tag="VQ526_001151"
     CDS             complement(3572..4312)
                     /locus_tag="VQ526_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2554879.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(4327..5640)
                     /gene="rsmB"
                     /locus_tag="VQ526_001152"
     CDS             complement(4327..5640)
                     /gene="rsmB"
                     /locus_tag="VQ526_001152"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2554880.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKRSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5633..6568)
                     /locus_tag="VQ526_001153"
     CDS             complement(5633..6568)
                     /locus_tag="VQ526_001153"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2554881.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGALFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6581..8977)
                     /locus_tag="VQ526_001154"
     CDS             complement(6581..8977)
                     /locus_tag="VQ526_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2554882.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQAHDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9043..9357)
                     /locus_tag="VQ526_001155"
     CDS             complement(9043..9357)
                     /locus_tag="VQ526_001155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2554883.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHQDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9382..10008)
                     /gene="gmk"
                     /locus_tag="VQ526_001156"
     CDS             complement(9382..10008)
                     /gene="gmk"
                     /locus_tag="VQ526_001156"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2554884.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10138..11751)
                     /locus_tag="VQ526_001157"
     CDS             complement(10138..11751)
                     /locus_tag="VQ526_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2554885.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11942..13039)
                     /locus_tag="VQ526_001158"
     CDS             complement(11942..13039)
                     /locus_tag="VQ526_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2554886.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQSMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVEAVATKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13049..13795)
                     /locus_tag="VQ526_001159"
     CDS             complement(13049..13795)
                     /locus_tag="VQ526_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2554887.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKEKKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(13898..14251)
                     /gene="rsfS"
                     /locus_tag="VQ526_001160"
     CDS             complement(13898..14251)
                     /gene="rsfS"
                     /locus_tag="VQ526_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2554888.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(14264..14764)
                     /locus_tag="VQ526_001161"
     CDS             complement(14264..14764)
                     /locus_tag="VQ526_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2554889.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEEHFADVL
                     DYKHIF"
     gene            complement(14765..15358)
                     /gene="yqeK"
                     /locus_tag="VQ526_001162"
     CDS             complement(14765..15358)
                     /gene="yqeK"
                     /locus_tag="VQ526_001162"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2554890.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(15358..15987)
                     /locus_tag="VQ526_001163"
     CDS             complement(15358..15987)
                     /locus_tag="VQ526_001163"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2554891.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16039..16350)
                     /gene="yhbY"
                     /locus_tag="VQ526_001164"
     CDS             complement(16039..16350)
                     /gene="yhbY"
                     /locus_tag="VQ526_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2554892.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(16564..17670)
                     /gene="yqeH"
                     /locus_tag="VQ526_001165"
     CDS             complement(16564..17670)
                     /gene="yqeH"
                     /locus_tag="VQ526_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2554893.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(17673..18263)
                     /locus_tag="VQ526_001166"
     CDS             complement(17673..18263)
                     /locus_tag="VQ526_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2554894.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(18215..19123)
                     /locus_tag="VQ526_001167"
     CDS             complement(18215..19123)
                     /locus_tag="VQ526_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2554895.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(19180..19740)
                     /locus_tag="VQ526_001168"
     CDS             complement(19180..19740)
                     /locus_tag="VQ526_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2554896.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            19879..21087
                     /gene="sstT"
                     /locus_tag="VQ526_001169"
                     /pseudo
     CDS             19879..21087
                     /gene="sstT"
                     /locus_tag="VQ526_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21155..22108
                     /locus_tag="VQ526_001170"
     CDS             21155..22108
                     /locus_tag="VQ526_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2554897.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            22253..22633
                     /locus_tag="VQ526_001171"
     CDS             22253..22633
                     /locus_tag="VQ526_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2554898.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            22732..22953
                     /locus_tag="VQ526_001172"
     CDS             22732..22953
                     /locus_tag="VQ526_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2554899.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            22970..23284
                     /gene="trxA"
                     /locus_tag="VQ526_001173"
     CDS             22970..23284
                     /gene="trxA"
                     /locus_tag="VQ526_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2554900.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            23591..24124
                     /gene="queF"
                     /locus_tag="VQ526_001174"
     CDS             23591..24124
                     /gene="queF"
                     /locus_tag="VQ526_001174"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2554901.1"
                     /translation="MKRQSALVVFSGGQDSKTCLFWTMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT         6594 a         5661 c         4796 g         7171 t
ORIGIN      
        1 attcgctaat tgtttttttt agggcgattg atttttactt ccgtcgcatc aatcattacc
       61 gtgtcttcag aactgagagg agttcttgaa atcgtaacac cacctttgaa caagagttac
      121 ttcaacccat tggctccgac ggagtaagtt gctttcgtgg ataccaaaat cagccgcaat
      181 ttcttcataa gtgcggtatt ctcgcacata tagaaagcgt tgttaattta tttatctcat
      241 ttttcagaaa attcttttat ttctgtaaag tctacgatac tcgatgtgtt tttatataat
      301 gatacagtct gagaatcact gttccgctag ccattccaat agagattacc aaagccaaca
      361 tgacaactaa ggtcgcactt gccagtgctt tattatagtc ccctgtcaca aaaaaggcag
      421 ttgttcggta ggagagataa cctggaacca gcggtgccaa aatggccaag ataaagacca
      481 cagcaggtgt cttataaaga atacttaaaa tctggctgac acaagaacca ataatggctg
      541 caatgaaggt agctacaatg acattggtcg gttccttgag caagagatag attagccaga
      601 cagtcatgcc caaaatccct ccaggtaaga gcatagaccg ttgcacattg agtacaatta
      661 aaaaagtgat aatggcaaga aaacttgcta ctgcttgtaa taaaaaggtt gttagtgtca
      721 tattagttca tcaataccaa ggcgacagaa gttcctgccc ctaaagcgag ggtaatgagc
      781 agggattcaa acatcttact cataccagag tttatgtggt tggtcataat atcacggacc
      841 gcattggtca aggcaatacc tggtacaaac ggcatgaccg caccagctat aatcaaatct
      901 gccgttgaag gaaaacctgt gtagcgagcc caaaactggg caattatccc aaagacaaag
      961 gctccagcaa aggctgtcac aaagggaatt cggataaatt tttccacata gagggaaaag
     1021 gcaaaaccaa ataaggtcgc cactcctgcc ccaagtgcgt cgtagatatt tccgctaaac
     1081 ataactgaaa agaaaggagc actaaaggtc gcagccagag ttacctgcaa cttagtatag
     1141 ggaaggggtt gagcttgcaa ggccgtcaat tgcttaaagg ctgtttctaa gtcaatctgc
     1201 cccccaacta gctgacgaga aatctggttc acatcgcaga ctttttcaat gttataagaa
     1261 gaggaggtca cgcgcttcat gcgcgaaata ttggtatttt caatagagaa aaagatagcg
     1321 gcaggcatgg caaggacatt gcaatccaca atcccctgcg aatgcgcgat tcgaatcatg
     1381 gtatcttcta cacgatggat ttctgagcca cttttaagga gaatagtccc cgctagcata
     1441 atcacatcaa tgacggcatt taattctttt gattcttcca tgctttcctc cttttatcaa
     1501 ctccctctat tctatcacaa atccggactc aaaaaaaatc tttgccatga aatcatgaca
     1561 aagattgatt actcattttg attatccatc tgcttttaag gagtagctga agttgtttta
     1621 ggtttgtaga ttgaaatctt gactctagtc ttattgaggt ctaccttttc acctgctcta
     1681 ggactttgtt caacaaccat gccttctgca ctacctgcag gcgctgtcgt cacttctaca
     1741 acttctatat tagcttcctt aatcccaaca atttgaatca aattgttctt agtaaactcc
     1801 aagctggaac caatgtaact cggcatggca acacttgtaa cttttttagc tactgtcaag
     1861 acaatttgag taggtttact cacatcataa gtcgttccgg cacctggact ttgtttcata
     1921 atcgttcctg gttcgctttc gctggactct tcttcctcta tcttaatcaa attctcagga
     1981 accttcttct gcttgagttc tgagattact tctgtagagt tccgtccaat atagttccct
     2041 aattgaatcg tcgtagcttt tttagctact gtcaaaacaa tttgagttgc cttgctcaag
     2101 tcataggtcg taccttctgg tagactttgc ttcaggaccg ttccagcctc actctcattc
     2161 gactcttctt cctcaatttt aatcaaatta tccggaactt ttttctcttt taattccgcg
     2221 ataacatcag aagatttccg gccgacataa ttactaattt ggaaggattg tttgcctgat
     2281 gagacaacca gattgatttt cgttccttct tttcgaccag ttccagcgcc aggatctgta
     2341 cggataatcc gcccttcttc caccttttca ctagcctctg tcttctcctc accaatctca
     2401 aaattggctt ttttgagcgt tgccttggcc tctgcaactg tctgacctgc cacatctgga
     2461 atggcaatgg ttgcaggagt tctggatagt atccaaataa gagaagctgc caccaataca
     2521 aggctggcca acaaaatcag gtaacgcatc ttaaatctat gttttttcgg tgcttgtggt
     2581 tggtaagttt cctctgtcac agcctggctt gggtttttga ttgatttgtg ttctgtctgc
     2641 gcttgaacct taggaataga tgtcaaggta ctttgagaaa ccttcggcaa ggtcttggta
     2701 tctgccttgc tcgtttcatc aaagattaac ttactttcat ttctacgatt gtaggacaag
     2761 ctactagaca agtccacata catctctgaa accgagcggt agcgattggt caacttttta
     2821 gcagttgcct tgataataac attttctaaa gcctgaggta cagatggatt ttctgcaata
     2881 acggacggca ggggtttctg gaaatgctgg agggcaatgg tcaccgcgct atccccgtca
     2941 taagggatat ggcctgtcaa catctcatag aaaataatcc ccatggcata gatatcactc
     3001 tgcacagtcg ccttagaacc acgcgcctgc tctggtgaca agtaatgaac tgagcccaac
     3061 atcgagttag tctgggtcag acttgtctct gcaaaggcta cagcaatccc aaagtctgtg
     3121 accttggcag tcccatctgg tgtcaaaagg atattttgag gtttcaagtc cctgtgaaca
     3181 attcctcgag tatgggccaa gcgcatagcc aagagaattt gtcccatgat acggacggct
     3241 tcttcattag aaagaggata atgttccttg atatagcgtt tgaggtctag tccagcaaca
     3301 tactccattg caagatactg ttgaccgtct tcctcaccaa tatctgttat ccgaacgata
     3361 tgaggatggt ctagatctgc catagctctc gcttcacgct gaaaacgagc tacagctatc
     3421 gggtccgtct ggtagttggt cctcagaacc ttcactgcca cttcttcccc atctaagatt
     3481 aagtctttgg ctaggtaaac atccgccatg cctcctcgac caatctgttt gacaatccga
     3541 tagcgtccgg caaaaatctt gccgatttgg atcattcttc atcctcctcg ttcatagaaa
     3601 caagggcaac cgtaatgttg tctaaacctc ctgcattgtt agcaaaacga acaagtgtct
     3661 ccgttttatc tgctaaagga atatcactgg ttacaatatc acgaatctca ctgcctgaaa
     3721 tcatgttggt caagccgtca ctattgagca agagatagtc acctgactca aggataactg
     3781 tcccaaaatc aggctgaatt tcatcttttt gcccaataga ctgggtgata atattttttt
     3841 gcggatgagc ttctgcctct tctggtgtca attgaccagc cttgagcaat tcattaacca
     3901 aggaatgatc gctcgtcaac tgatggtatt cttctccacg aatcaagccg atacgcgaat
     3961 caccaatatg agcatagata gcctgattat caataatagc aaggacttcc aaagtagttc
     4021 ccatgcctct gtaagcttca tcctgaccaa gctggtgaat cttttgattt tcaatttcta
     4081 ggtaatgggc gaaccattca cgcacttcat tgactgtatc gatctgggta tcaacccaag
     4141 ctacacccag gtctgtgacc gccatttcac tagcgatatt ccctgcacga tgacctccca
     4201 tcccatcagc taaaataatc atggtacgtc cagctctatt gacatagtgg ttgacatagt
     4261 cttggttatt tgttcgtttc tgaccaacat ctgttaataa tgaaatttcc atgtgtcagt
     4321 tccttcctaa tccgatatct tgcgaaattg actgatgaag aatccatcac ttccatacaa
     4381 ttcaggtgta atgaggatac agccgtcttt catgatatcc ttacattcat gttctagttt
     4441 tacctgctcg aactcgggat gactctctaa aaaggcctta acgacttgaa aattctcctc
     4501 tgagacgata gtgcaggtgc tataagttat tataccacct ttgcctagta tttgacaaac
     4561 actacctaat atttctaact gaatttcctg caaggacgcg aaatctgccg tttctttatt
     4621 gtatttgata tctggttttc ggcgcaaaag accgattcct gaacaaggag catccaccaa
     4681 aatcttatca aaggaatcct ggtcaaaaaa ctcatgcacc tttctggcat ccaatttttg
     4741 agtttgaacc cgatctgcaa ctcccagacg ttgggcattt tcttgaatta aatccaactt
     4801 gtggtcgtac aagtccagag cagtaacctg acctgtcgta agataagagg ctatatgggc
     4861 tgttttccca cctggagccg cacaggcatc aagcactcgc tcatcacctt gtaaatcaag
     4921 cgtcggagca accagctgac tggactcgtc ttggatggta atggctccat ccgcaaacaa
     4981 attatgccct gcaaaatgcc cttgctcctt aaccagacca gtggttgcta aaagggaatt
     5041 attcgcctcc aacaaggctt ggatttcctc ttttcgactt aggtctgtta cacgaatact
     5101 agctttgttt cgcactaaca ggctttcaaa gatggctttt gctctctcct ctccgtattc
     5161 ttccttgagt ttggcaacta gccaaactgg gagagaatag gcaatggagt cacgcttgtt
     5221 ttttcgcttg atgctagcaa tatctggcca gccttcacgc aagatacggc gaaggacagc
     5281 gttgaccaat ttttcactgc cttttttacg gagtttagcc aattccactg cttcattgac
     5341 cacagcatga tctggaatct tgtccaaata gcggagttgg taggcactca tgagaagaag
     5401 gacatagagc cagctgtcta actggtctct gtcttcgata aagtgggata ggtaccattc
     5461 cagagtcagt ttacgggcta ccgttccata gactagctcg gtcactaagc ccttgtctgc
     5521 tgcagagagc tgactcctct tgaggtgttt atttaaggca atatttgaat atgcttgatt
     5581 cacaaaaaca tcctctagca ctgctagagc taaacttcta gccgtttcta ctttagtcac
     5641 caaatcgttc tcctacagtc aatgtacgtc caactccgtt gaggaaggaa gcaatgtcca
     5701 tcttaggctt accagctggc tgcacttgtt tgagggatag agccccttca gccgttgcga
     5761 caatcaattc tttcttgcag atagagagaa tctcacctgg atttccctga ccttctactg
     5821 gtagggcttc ataaatctta aagcggtcgc ccttaaggaa agtatgggca acaggccagg
     5881 ggttcattcc acgaatttgg ttaaagagtt gacgattggt tttgttccag tccagttttt
     5941 cttcctctgg ctttatattt ggagagaagg taacctgact cgtatcctgc ggttcaggtt
     6001 tgatatcacc agcaatatag gcaggcagag tgtccaaaag caaatcacga ccaactagcg
     6061 ccaatttttc aaacaaggcg ccaacattgt cctcatctgt gatcggaatg ctgcgacgag
     6121 aaatcatatc tcctgcatcc atttccttaa ccatttccat gatggtcaca ccagcttcct
     6181 catccccttg aatcaaggca taatggatag gcgcaccacc acggtgtcta ggaaggaggg
     6241 aggcatgaac gttgacagca aagtccatgc tatcaaggag tttgcttggg agaaactgcc
     6301 caaaagcagc agtcacaatt ccatctgctc ctagcttcat aagatcttcc atctctggac
     6361 ttccagataa tttttcaggt tggtagatag atagtcctgc ttccttggca gcctgcttga
     6421 ctggggtttc ttggataact tttttacgac caacagcacg gtctggctgg gtcacaacgg
     6481 ctagaatttc gtaacggtca tctgtcaaaa gtccttttaa gactgttgct gaaaagtcgg
     6541 gggtccccat aaagattagt tttgtcatat cttctccttc ttataaaaat tgctgcggct
     6601 catggtcaat gctgagacgg agctcactat tttcccgttc ttgagtcaag gctaaaacct
     6661 ggttgagggt cggccccagc tcatcctcta aacggtattt aattaaaatc tggtaatgat
     6721 agaggttgtg ggtacgggca atcggttttg gcgttggccc cagaatggga ctggtctctg
     6781 acaagcctga ccgcaaaatg ttcatgactt cataggcacg tttgaaaacc tcttcttctt
     6841 tcttgtgaga aagggtaata ccaatcgtga aatagtaagg tggatagccg agttgtcgtc
     6901 tgattcccat ttcataggca taaaaacctt cgtaatcttg atccttggca aatcgaatag
     6961 catagtgctg cggattgtag gactgtatca agacttgacc tgctttttca gcccgacctg
     7021 ctcgacctgc cacctgagtc aagagctgga aggttctctc agaagaacgg aaatcaggca
     7081 gattcaaggc cgtatccgca tttagaactc cgactagggt aacattggga aaatccaaac
     7141 cctttgcaat catctgagta ccaagtaaaa tatccgcttc ccctcgccca aactggtcaa
     7201 gcaaggcttg gtgactgcct ttctttcgag tcgtatccac atccatcctc agaatgcgag
     7261 cttggggaaa gagttctgct agctcatcat aagccttctg agttcccgtc ccatagtaac
     7321 gaatactgcg gctcttacag ttaggacaga cctgaggaat atccttcgag aaaccacaat
     7381 aatggcagtt catagtcttg gtatccatat gcaaggtcag agaaatatcg cagttgggac
     7441 aagtatccac cgtcccacac tcccgacaca tgacaaaact agaataacca cggcgattga
     7501 gcatgagaac cacctgctct tttttaacca gacggtcttg gatagcctct agcaaaggag
     7561 gcgtaaagtt tgacgtctca ttttgtccga tatagtctcg aaagtcaatc acttgaacct
     7621 cagggattgt agccaaagga ttggcacgtt gggttagacg taagtgttga tagacgcctt
     7681 tgccagcacg cgcccggctc tctaagctcg gcgttgcaga tccaagtacc agagttgctt
     7741 gattatactg agcccgtaaa atagctacct ctctggcatg gtaacgggga ttgctgtcct
     7801 gcttataagc cgcttcatgc tcttcatcaa taatcatgac acccagattt ttcagaggag
     7861 caaagatagc agatctggca ccaacaacaa cttgggcatc gccacgctcc accttgcgcc
     7921 attcatcata cttttcacca ttggataatc ctgagtgaag aatggctacc ttgtccccaa
     7981 agcgtgctat aaaacgctcg gtcatttgag gagtcaagga aatctcaggt accagcaaaa
     8041 tagctgtctt gcccttatcc agggcacctt ggataatctg caagtaaacc tcggtcttcc
     8101 cacttcctgt aatcccttga agtagaaagg gaggttgaga actgccaata gaactcacaa
     8161 ccgcatcatg cgcctgtctt tgttctggat ttaactccaa aggtctactt gcttcaattc
     8221 cttcaaaata agcagccgag cgttgaactt ccttttggac tatggtaaca gcaccttgat
     8281 ccacaaagaa gttgacttgc tctcgcgagt aggactctaa caagctagcc aaggaagcgc
     8341 tctctggatg agacagcaga taatctctca gttccaactt tttcttggca cgtgtagaaa
     8401 tctcaacacc ttctaattgg gcatggtcaa cctcatacca agactgggtc ttgaccttct
     8461 tttgatcgac tgcctgatat tccagaccaa gcaggccttt tctagtcaaa cgcatcattt
     8521 cagcttgctt ggcaaggtct agtgaagaaa aggctagcga atcttctgaa ccaaacaggc
     8581 gtactcgttc ttcctgactc aagccttcca gaggatagag aatcttgtca tagctagaat
     8641 tcagaaatcc tggaagcatg gccttgagga tagaaatttt gtaggagaag acagatttgc
     8701 gtaactcctc agccagccag agttgttctg gcgtgagaac aggagaaaaa tccagcacct
     8761 ctgcaatatc ttttaaatct tgctccatct cttctccatc tgattgggac tccaaaccaa
     8821 gaacaatccc ttgaatcagg cgattaccct taccaaaagg cacatgaacc cgcatcccaa
     8881 cttccagcat tccctcaaat tcctccggaa tcctgtaact atagggctgg tccgtctgca
     8941 tcaagggcac atctacgata atcttagcta gggccatctt ctcacctcct ccttgtcagt
     9001 acattcttgc aatagaaaaa ataagattga gtccccccaa ccttaaattt tttcaccatc
     9061 ttctttttct ttagcaattt gctctttgat tttcttttct tcttcttctt tgcggcgttt
     9121 ttcttcttcg atacggcgac gcactgcttc acgttttcct tctggatctt ggtgaattgt
     9181 aacgtttcct gattcgattt cttctaaagc gcgaagagtt gatttttcag acttgaaacc
     9241 ttgagttgct ggggcacctg cttccaattc gtgggcacgt tttgcttcca agattacgag
     9301 tgaatatttt gaaggaacct tgtcgagcaa ggtatcaata gagggtttta acatcatttg
     9361 cttgtaccta ttttctaaat tttatcgggt agttggagat tttggtaaca tctcctgata
     9421 gtgaccaatg acacgatcca cacagaagtg ttctgcttca atcacacatt tgacacgttc
     9481 agcagctagg ggtacctgat cgttgacaat cgcataatca tactcacgca tgagggcaat
     9541 ttcttccttg gccttttcga ttcgttgggc aatcacttct gcactatctg ttccacgacc
     9601 taccaagcga tcttgcaatt catccaaatc tggtggtgtc aggaagataa agacagcatc
     9661 tggaaccttt ttcttgacct gaagagcacc ctgaacttca atttcaagga aaacatcgat
     9721 tcccttgtcc aaggtttcat tgacataggt cagaggagtt ccatagtagt tgccgacata
     9781 ttctgcgtat tccaacatct gtccttgacg aatcagctct tcaaattctt cacgagtacg
     9841 gaagaaatag tcaacaccgt ccacttctcc aggacgttgt gcgcgtgtcg tcatcgatac
     9901 agaatattga aattggtttt cagaactctc aaaaatctct cttctaaccg ttccttttcc
     9961 aacccctgaa ggaccagaaa aaacgattag taagcctcgg tctgccattg tgtctccttt
    10021 tagtcaatct gtgaaataac atttctctag aataatggca aaaagccaga ttatccttta
    10081 cagtctttct atctagtgta acaaaaaagc agtaattttt caactgctct ttcttattta
    10141 tttagcataa tctactgcac gaagctcgcg aatcacggtt accttgatat ttcctggata
    10201 atcgagattg ttttcaattt tcttacgaac tttgtgagcc aagattgtga ctttgtcgtc
    10261 cttgattttt cctggattga ccatgatacg aatttcacgt cctgcttgaa gggcaaagct
    10321 agtttgcact ccttcaaagc cgttagcaat ttcttccaaa tcatggagac gcttgatgta
    10381 gctttcaaga gactcactac gagcacctgg acgggctgcg ctcaaggcat ctgctgcagc
    10441 gacgataact gctatcacgc tctcagcttc aacatctccg tggtgactag caatcgtatt
    10501 caccacaact gggggttcct tgtacttacg ggccaattcc ataccgattt caacgtggct
    10561 accttcaacc tcatggtcaa tggctttccc gatatcgtga aggaatccag cacgacgggc
    10621 aagagccgca ttttcaccaa gttcgctcgc catgatacca gccaacttag caacctcaat
    10681 cgaatggcgc aaaacatttt gtccatatga agtacggaac tgcaaacgtc ccataatctt
    10741 catcaagtct ggatgaaggt ttggcgcacc aatttcatag gcagcagcct caccgtattc
    10801 acgaatctta ttgtcaatct cttgacggtt tttctcaacc aactcttcga tacgagctgg
    10861 atgtatacga ccatctttga gcaacatttc catagtcata cgggcaatct cacgacgaat
    10921 cggatcaaat cctgacaagg tcaccacttc tggtgtatcg tcgataatca catcgacccc
    10981 tgtcaaactt tcaaaggtac gaatgttacg accttcacga ccaataatgc gtcccttcat
    11041 agtatcgtct ggcagatgaa ctgttgagtt tgttgactcc gctacatatt caccagcgat
    11101 acgttgcata gcttgaacca agatgtcctt ggccattttg tcagaacgtt ccttgacctc
    11161 ttgctcagct tcgcgaatgc gactggcaat ctccctggtc aagttttcct ctgtctgagc
    11221 caagataata tctcgtgctt ctgcctgaga cagagcacca atacgctcta gttctgcttc
    11281 tttttgtctt tcgacttcct ctaattgctc ttcacgcgca tcaaggtttt tcgctctatc
    11341 agaaatactt tgttcttttt gttcaagtgt ttgttcttta ctcgtcaaat tgtcgtcctt
    11401 acggtcaagg ctagtagctc tctctgtcaa acgactttcg atttgtttga gttcttgacg
    11461 ttctgatttg aattcagcgt ccacttcttc acggtatttt ctggcttctt ctttggcctc
    11521 caatagtgct tcttttttaa gagacttgct ttcacgttta gcttcattaa caagtaaatc
    11581 cgcttcacgc tcagcttgtc cacgtaaatt agttgcttct tgttcagcat ttaaaagcat
    11641 caactccgca gcttcctgag atgatttcat cttagctgag atgctgacat atccaatgac
    11701 taaaccaatg atgacggcaa aaacagcaat cgcaagcgac atgatctcca tgtttttacc
    11761 tcattttatt gttattccga atgacataca ttcttttaca ttctaccata aaaaagtgat
    11821 tttcacaaac ctaaaataga atatgttttg gggaatttgg aacacatttg ccaaaataaa
    11881 cttgtttttt agaaatagta gtttagtaga gacttgagaa aaagcctacc tttcagtaga
    11941 cttagtttgt ttctattcta atcggcactc ttccaaaatt ttgctctgct atacttggct
    12001 ttcctagttg gtaaatctgg tctgcttttt gggtcatagc atcccaaggt tctttgccaa
    12061 ttcggctgac tagattgacc tgtcctttca gagacttgag atgttgcctg cctttttcag
    12121 taaatccaag gacatgaatg gcttctggca agacattttc tctagcctgc accaaaatat
    12181 aggtcaatag gcgtctgaca cgcgccttgg tgtaacgttt ggtcgcaacc gcctcgacca
    12241 actcttctac agactgggct gttttaatag cttctttaat gcgcacagcc atttcttgat
    12301 tgacctgata gatggtggtt aggtcgggat ttgacaagat ttgatagcgg agcaagggaa
    12361 aatagtcttc ccagatcacc ttactggcct gctcaaatag ggcaacagaa ggcataaagc
    12421 gttctaagaa atcttggtcc ttctgatgtt gacggagggc tgtcgccgag gcaaagtcca
    12481 catctttatt cacagaatgg taacctgccc cctgacgctg aatcggatgc agcttgatat
    12541 ttcgtcctgc aaccgccttt gcataggcca gagcaagaac atgattgggg gtattacctg
    12601 aaaaatcaag accagcaaat tccttccaca tagattgggt tttctgggga taggaaaggg
    12661 aatcaggcag attttccaca aatttctcca tctcagcacc tttctctgtg tataagtcag
    12721 cgattttctg ataatctcga acttcctctg tcccaaaaac tagactatca atgcccaagc
    12781 gatccaagat atccacagct ccttgaccaa agaaatcagc tgcctgaaca ctgactaaaa
    12841 agggcaattc cactaccaag tctgcgccat tttccagcgc catctgggcc cttgtccact
    12901 tatccacaat agcaggctct ccacgctgca tgaaattccc tgacatggca acaattttca
    12961 gtccctctgc ctgatccagc aggtatttat ggccattatg aaaaggattg aactccgcga
    13021 taatacctgt gatggtcatg ctaatctcct acttctgcgc cacaaaaaac caacgggtgc
    13081 tagtttctgt tggttcctta tcctcaaagt ctgcatagag tttgaaggac ttgaatccag
    13141 cctgttccag taagatatca taggtcaaga tctcataggt cctctcctca tgtacttcat
    13201 cgtggcgact aaaggaaccg tcagcctcct tgataaagaa agtcagctca tgcacgatgg
    13261 agtgaggagc ttcgtcctca tatgtatccc aaagcatggc aaaatcttcc gcattttcat
    13321 ggtaggaata gccagggaat acttcatctg tctggtaggt cgagtgcacg tcaaagatga
    13381 aaactccctc ttcattcagc gcattgtaca cgtccttaaa gacgtcccct acttccacct
    13441 catcctgcat atagcagata gagtccgaat aacacgtgac aaaatcgtat ttccctgcct
    13501 tggacaaatt cagcatatta ccttctataa aagcaatctt ttgcttggct gaagttgctc
    13561 tcttctccgc aatcttcaac atatccgcac tcaagtccag tccagtcaca tcaaaaccag
    13621 cctgagagaa gcgcactgat tgaattcctg tcccacaagc cagttccaag agtttctttt
    13681 tctccttggt cttaggcaaa tgacgcagag aaaaatttgt ccatttgtcg tataaactat
    13741 cgtccatgac cgcatcatag accgccgcaa aggtttcata agtcgccata atcatgatac
    13801 taagagctcc atggtaaaca ccactagccc tttacctttc tatcaatttt tctaaaataa
    13861 gcaaagaaac ccagttgact acaactgagt tcatcttcta agcaagagct tctgaaatat
    13921 ctactgaatt cgcctcatgc catagcttct ctagattata atgggcacgc atttcttctg
    13981 aaaagatatg cacgacaaca gcaccgaggt ccagtaagac ccagcctcca gctgcatcgc
    14041 cttcgacatg gctaccttta aagcccgctt gagctacttt ttcacggata ttagctgcga
    14101 tagcgtccaa ctgacggcta ttcattgagc tagtgatgac aaagtagtcc gtcacactag
    14161 tcaaatcttg tacatcaagt gcgaggatat cctccgcacg tttctcatca gccgctttca
    14221 cgactagttc tagtaattct ttttcgttca tttaatcctc tttttagaaa atatgtttgt
    14281 aatctaggac atctgcaaag tgctcctccc aaatgtcctc ataaaattca ttaatcgttt
    14341 ctgctggaat gtcatcccca tcaaaggttg tgacagcacc ttctggaaca ataagctgat
    14401 agccatattc aaaggcaacc ttgacagagg tatccacaca atattctgtc tgcataccac
    14461 ataaaactaa tttttcaatc ccctgtttat ccaagtattc ttttaagcca gtttctttga
    14521 aaatactgtt atacttcttc tgaaagacct tttcagcagg ttttcgattt aaaagcgcag
    14581 ataactgcca atcttctgat gtttgagctt cagaattctc aatatgttga acatagataa
    14641 tttcaatatt cttgcttcta gcctggtttt gtaaataaga aattttttcc aatagacttt
    14701 ttgtctggaa cccagtttct actaaaacat tctgaacatc aatgattata aaagccgtct
    14761 tcatttagtc ctctttcaaa tagtgcacaa aggcgttata ggtttcaagg gtttggggat
    14821 agatggggaa tccctgatga gctagatact ccacggtacg agctgtttcg taggccaccg
    14881 ccttattgag cgatagactt gcaatctcac gcgccacatc tactccagga aaggctcgat
    14941 tgtgctcgat atagtctgcg acgtagatga ccttatctag gtctgtcatt tgaccagcac
    15001 cgactgtatg gacttcaatg gctcgcagga tttctgaatc atgcaaatcc aaatcttcct
    15061 gaattttgta aattccgacc ataccatgcc agacattatt accccagttt ttgaggtcga
    15121 aatctagctg ataacggtca atcaagtcta gaaattcctg atctgacaac tttttagcat
    15181 agtcatgaag aagacctgct agacttgctt tctcaacatc tacaccaaag cgctgagcta
    15241 actccatagc ggcacgctcc acacctagac aatgggttag acgtttctca ggtagaagct
    15301 ctgccatttt ttccaacaag gcctcacggg agcagttgat atagtcttga taggacatca
    15361 gtagagcccc tccttctcga tgtagtctag cactggctga ggtaggagaa agttgggttt
    15421 ccgaccttgg gcaatgaagt cccgcaccat gctggacgag atatccatga gcggtacgtc
    15481 cacccagata actggatagg aagtccctgc cttgtagcgt ggacgctgaa cccccacaaa
    15541 ctgaaccatg tcaaccagtt catcaattcg gtaccactta ggcagatagt caaccatgtc
    15601 ggcaccgatg ataaagtaat aatccgtatc tggatgttgc tctgtcaaga ttttcatggt
    15661 gtcgtaggtg taggaaatac ccttgcgctc caactcaatg gtttcaatga ctaggccgtc
    15721 aatcccctca attgccaact caagcatctt gagacgatgg tgttcaggga tggtttcctt
    15781 tttatcaacg tgaggaggtt ggtattcagg catgagaaga acttgatcca gtcccaactg
    15841 ttgccgtact tgatccgcaa caatgagatg ggcattgtga acagggttaa aattcccccc
    15901 taaaatccca acttgtttgc gttttttctc cttgatttct ggctccaact ctaccttggt
    15961 aaagggagtc aatagttcga ttgtcatagg ctagtcccct ttatttctct ataaaaacag
    16021 ttatttggag tttcggtctt agatttcttt gactttctta gaaatcttgc gattttcttt
    16081 cttgctagat tgtttaaaca aaatcaagat gcgtcctatt ttttggactg tatccacacc
    16141 gatttcttct tccaaaattt cagctacttc gtggatattt tcatctgtgt tttgtaagag
    16201 agtaaccttg attaattcac gcgcatcaag agcttgacgg acgctggttt tgatttggtc
    16261 gttgagtcca tttttcccga tttggatgat aggtttgagg gtgtgtgcct ggctgttgag
    16321 gaaggcacgt tgttttgatg ttaatgacat aattttcttc cttgtttttg gatagttatt
    16381 ttaggtggtg agggacggtc ggaactaatt ttccaaagat ttcatctttg aaaaatggat
    16441 ttcctgacct cacaattgag ccttggtctc aattgtgccc cttgccaatc tacagattgg
    16501 caatcacacc acggcaataa ctatcgctat atgagctcac tttgttcgct cagcgatttt
    16561 gttttaaata attgcttttc gtgtgacgac ggcgacgcct tctggtgccc agacggcgac
    16621 ttttgctatg cctgttacac gaatccagcc taggcctgag atgactaggt ctgtcttatc
    16681 tttaatggta aagacatgct ggactagcct tgggaaatct tctttttcct tgctatttgg
    16741 tggtgtcaga agagttccca ggtgcttatc gtagaaagca ctagctcctt caagcttgct
    16801 acgatggagt ttgagttcat tatcaaagaa agcagtaaat ccttgctttt ctcctgctat
    16861 aaagtcaaag cgtcccaaac cacctaaaaa tagggtttgc tcaggattaa gctgataggt
    16921 cttaggcttg atttcctttt taggactgac atacttgagg tttttggccg tcaagtagtg
    16981 agccatctgg tggcggtgga taattcccgg cgtatcgtaa atataagatc cgtcgtcaag
    17041 cggaatctct attttgtcca aggttgtacc tgggaagcgt gaagtagtga tgacattctg
    17101 atcacccgtg atttcttgga taatagcatt gattagagtt gattttccaa cgttggtcac
    17161 accgaccaca tagacatcgc ggcccttacg gtagtgttca atcttgtcaa tgacttcctt
    17221 aatggcatgt ttattttgtg ctgaagttag gaccacatcg actggacgaa gaccttcttc
    17281 atgggcacgt ttcatgagcc actggctaat cttaccagac ttaactgact taggaaggat
    17341 atctttttta tttcctacca agaggacatc attgcccgag acgaaacgtg gtaaacctgg
    17401 gatgacagat ccattaaaat caaagatatc aatgacattg accactaagg catcactgtc
    17461 tcccacctcg tgcaagagct tgaggaaatc atcgttcgtc aactggacat ctgtgatttc
    17521 attgtagtgg cggagacgga aacagcgttg gcaatagact tcgccagtct ccaaaccttt
    17581 ttcaagtgcc gactgggggg taaaaccaag accagcttta tctgtcgtct gaatggttgc
    17641 tccacaacca atacagagaa tttcttccat agttaaattc cttttttata tgtaatcggt
    17701 ccgtactttt cagtgatttt tctcataaca cgacgctcac gagttcggtt aatctgcgtt
    17761 ttgattgagt catgttggac caagggtttg actaaaattg accgaatccc tgcacggtgg
    17821 gctgctcgta tatctgtcat gagctggtcg ccaaccatga ccacttcctt tttgtcatag
    17881 tggaattcct tcatagcacg gtcaatacca aatgtgaagg gcttcaaggc ccagtaaacg
    17941 taatcaatcc caaatttctc aactgctcgt tgaacgcgtt ttttggtgtt atttgacact
    18001 acgataatgc caatacccgc gtcccgaagg tcatgtagcc attgcttcat ctctggcgtt
    18061 ccatcagggt tgttccaagc aatgagggta ttatccaaat cgaccaaaac agccttgatt
    18121 ccctgcgcct gcaggcttgg gactgtcaga tcatagactg cttccacagc aaaatctggt
    18181 atataatttt caatcgccat tctggctcct tttcttaact ttttttcgca attttcctta
    18241 gtaacaagga taaaacaatc cacaaacctg cactagccaa actgatatag agccaagcat
    18301 ggggctcatc tgttaaaggt aagggaacat tcattccgaa aaagcctgtc acgactgcca
    18361 aaacagctag caagactgaa atgatagtca aggttgtcaa attgtcattc agattattgt
    18421 ttagaatatt gttgtaagag gctgaaagct gctgtaaaat ctgagagatt aggtctgtca
    18481 tggataccag ctgatgagcc tcaatcatgg catcatcaaa ctgttctctc tcaatctcat
    18541 caaaactacg atacaaggca tgaccttgaa tatgctctaa caaaatccga ttttgtttgg
    18601 cagctgccgt cagataaacc ataccagtct ccaaatcaga cagggcaaag aggtttttct
    18661 tggtagttcg ctggcgcaag aggtcattga cctcatccct actcttgtcc atctgctcaa
    18721 tgacaggata gtaggcattg ctgataattt ccagactggc aaagagaaac ttataaatcg
    18781 aaagcgtgtc atggctctcc agataacgag tcatctgttc aatgacatag gcgttcttgg
    18841 tattactaat ggtaatcagg cgacgatgct cgacaataaa ggtcatggga aaagtctcat
    18901 agtaggcctt gtcctttttt acgtctagga cattatagat aaaggtaacc gtcccacttt
    18961 cacggtggta atccatgtgg gcgcgctcgt ttctatccag tgcgtattca atggtttcct
    19021 tgtccaaacc gtaaatttca gaaaggtctt ctagtttatt caactttcct aggtctaggt
    19081 ctatccaggt acaaccattg cccaactgct tttctaaaac catcttttct ccttattaaa
    19141 ctccctctat tgtaccacaa attacttaaa ataacaggtc tagtctgtgc gtgagaaatt
    19201 gctaacaact gtgatatttc ggttagggac aaagtgaagg gcttgctcct cgctacgaag
    19261 ctgtgtcgta cgaattccca cactgacaac cttacccgat acagtaatcg gtccatttgt
    19321 cagaacgacc tcatctccca catccagttg acgttcaaag aggatgaaaa agccattgat
    19381 gacatcagac agaaagcctt gggctcccat accaatcgct accccagcaa taccagctcc
    19441 agccagcaaa ctagaaactg gcaaacctaa aatcgacaaa atgcagtaga gtaaaaagaa
    19501 atatagcgta taattaaaca cattttctag taaacgtgag atggtttttt ggcgtccgac
    19561 atcatgacga gacattttta gagaaggttt gacaattctc tgcaccatgg tatgaagcat
    19621 ttttttagca atataaaata caatcaaaag cagtaaaaga gaaatgacct tggttagaat
    19681 attctcgata atggttgtca catctagctt ttcaatataa gcctgaataa atttttgcat
    19741 ataaaactcc tttatttcat tataccatat tttcatcagt ctcattctcc ataaatattc
    19801 aaaaaataga cagaaaattc ttgattttgt ctatcattta tactataatc ataatcacta
    19861 cacaaaaagg agattattat gaaaagaatc attcatgctt ggaataaggc aagccttgtc
    19921 aagcgtatct tgattggtat gctttgtggg gaatcctagg actgaccctt cctaatctct
    19981 cagggattgg gctactcggg gatttattcg ttggaggtct aaaagctgtt gctcctatcc
    20041 tagtctttgc cctcgttgcc aatgcccttt ccaacatcaa aagggacaag atagcaatat
    20101 gaaaactgtt gttttcttgt atatcctatg atagggactt tcgccgctgc tcttgtagct
    20161 gtactagcaa gtttcatcgt ccctattgaa attaccctaa atagtgccaa tactgaaatt
    20221 gcaccaccag atgggattgg gcaggtcctc agcaacctct tgctcaaact ggttgacaac
    20281 ccagtcaacg ccctgcttac tgctaactat attagaatct tatcttgggc agtcattttt
    20341 ggaatcgcta tgagagaagc cagtaaaaat agtaaagaat tgctaaaaac tatcgctgac
    20401 gtgacttcta aaattgtcga atggatcatc aatctggctc catttggaat ccttggtctt
    20461 gtttttaaaa ccatttctga caagggagtc ggaagccttg ccaactacgg tattttattg
    20521 gttctattag taacgactat gctttttgtt gcccctgtgg tcaacccttt gattgccttc
    20581 ttctttatga gacgcaatcc ttaccctcta gtttggaact gcctccgtgt cagcggtgtg
    20641 acagcctttt tcactcgtag ttctgcgact aacattcctg tcaacatgaa actctgccat
    20701 gaccttggac tcaacccaga tacctattct gtttctatcc cactcggttc tactatcaat
    20761 atggctggag tagcgattac cattaacgtt ttgacccttg ttacagttaa cactcttgga
    20821 attcctgttg actttgccac agcctttgtc ctcagtgtgg tagcagctat ctcagcctgt
    20881 gatgcttcag gtattgccgg aggttccctc cttcttatcc cagttgcttg tagccttttc
    20941 ggtatttcta acgatattgc catacaaatt gttggggttg gttttgtgat tggtgtcatc
    21001 caagactcat gtgaaacagc ccttaactct tctacagatg tcctctttac cgccgttgcc
    21061 gaatacgcag caacccgtaa aaaataactc atcaaggcaa gcctgctcat gtcttgtctt
    21121 ttacgctttt attctaactt attaggaaat tcttatgtct attagccaac gtacgaccaa
    21181 gctcatctta gctacctgtc ttgcctgcct gcttgcttat tttctcaatc tttcgtcagc
    21241 agtttcggct ggaattatcg ctctcttgag cctatctgat acgcgtagaa gtactttaaa
    21301 actggctcgc aatcgtcttt tttctatgct tctagctctg gctatcggcg ttctagcttt
    21361 tcacttgagc ggatttcata tctggagtct cggcctctat ctggccttct acgttccttt
    21421 agcctacaag atgggctggg aaattggcat cacaccaagc acggttttgg ttagccatct
    21481 cttggttcaa gagtcaacct ctccagacct cctagtcaac gaattccttc tctttgctat
    21541 tggtacagga tttgccttgc ttgttaatct ctatatgcct tcacgagaag aggaaatcca
    21601 gcactaccac acgctggtgg aagaaaagtt aaaagatatc ctccagcgct tcaaatacta
    21661 tttatccaga ggagacggac gcaaccgagc acagctggta gcagaattag acacgctttt
    21721 gaaagaagcc ctcagactgg tctatttgga tcactctgac cacctctttc accagaccga
    21781 ctaccatatc cactactttg agatgagaca acgacaaagt cgtatcctga gaaacatggc
    21841 ccaacagatt aacacttgtc accttgccgc cagtgaaagc ctgatcttag cgcaactctt
    21901 ttcaaaaatt gcaggtcaac tgagccagac caatcctgct tctgatttgc tagatgaaat
    21961 tgaacgttat ctggaagtct tccggaaccg cagtctgccc aagacaagag aagagtttga
    22021 aacccgcgcc actcttcttc aactcctacg tgaagccaaa accttcatcc aagtaaaagt
    22081 tgatttttac caaaaatata gacagtaaaa aagaaaacca ctaaaaaaca gagcttgcca
    22141 gcattcttga aaagtagtat aattattgca tgcgcaatca tcttatgacg caaagactgc
    22201 ccgttaatgg actttcttct ataattatga ctctaaaaag aaaggagaca ctatgaccta
    22261 tttggaaaaa tggtttgact tcaatcggcg tcagaaagaa attgaaagtc tcttggaaga
    22321 gaccattgcc caacagagcg agcaaagtct gaccttgaaa gagttctacc tgctctacta
    22381 tctggacttg gctgaagaaa aatctctacg ccagattgac ctgccagata agcttcatct
    22441 gagcccgagc gcagtttcta ggatggtggc tcgcttggaa gctaaaaatt gcggtctact
    22501 cagtcgcatg tgttgccatc aagatagacg ctctagtttt atctgcctga caaatgatgg
    22561 gcaaaagaca ctggcctcgc tacaaaagac tgtcgaagaa agcttggaag caggtttgga
    22621 tttcttgatt taagatgatt ttaaaaaaag ttgcgtgcgc aatcattttt cttgacattc
    22681 tcttttataa ggagtaaaat aaagttagca ttaaacaaag gagttttaaa catgattgaa
    22741 attacctata tagatgccag caagaacgaa agaactgtga ctttcgagtc ttatgaagac
    22801 tttgaacgtt cgcaacaagc ttgccttatc ggcgtcgcag actactaccc tgtccaaaaa
    22861 ttaacttaca agggtcataa tttggactac catgggactt atggagatat cttcttctat
    22921 ctcatgaaac aagatttaag ccaatataac taaaaaaagg agaaatacaa tggcaaaagc
    22981 aattacagat gcaacattcg aacaagaaac aaaagacggt ttggtcttag tagacttctg
    23041 ggcaacttgg tgtggtccat gtcgtatgca aggtccaatc ttggacaaat tgtctgaaga
    23101 actttcagaa gatgtcttga aaatcgttaa aatggacgtt gatgaaaatc caaacacagc
    23161 tcgtgctttt ggaatcatgt ctattccaac tcttctcttc aaaaaagacg gccaagttgt
    23221 caaacaagtt gcaggtgttc acacagcaga acaaatcaag gccatcattg ctgaattgag
    23281 ctaatcaaac gagagaccaa gtactttctt tggtctcttt tttcttgccc tttgccattt
    23341 ttcaaaaaat atgctagact atagtagatt gaaactagaa tagtacatct ctacttctaa
    23401 aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttctt atttcatttt
    23461 actatataaa tagaatatta cgatatttgg gatatgccat cgctaaaaaa aacctcgatt
    23521 tggtattttt tagctcccta atgggagctt tttacgtgct ctgaacattt cccattttgg
    23581 aaggagtact atgaaacgtc aatcagcctt ggtcgtcttt agtggtggtc aagattcaaa
    23641 aacctgcctt ttctggacca tgcaacacta tgaaacagtc gaagctgtca cctttgccta
    23701 cggccaacgt catcacctcg aaattcaaat tactagagaa atcgctaagg aacagggcat
    23761 tcgtcaccat atcctcgata tgtctctgct gggacaaatc actgctcagc cagactttgc
    23821 gacgattcat atttcctaca ttcctgacaa gctctgtgtc gagtcaaaat ccctcaaact
    23881 atatctattt agctaccgaa accacggaga tttccacgaa aactgtatca acaccatcgg
    23941 gaaagacttg gtcaacttgc tagaccctcg ctatttagaa gtctggggaa aattcactcc
    24001 gcgcggtggc atttcaatcg acccctacta taactacggt aagcaaggaa ctaagtatga
    24061 gggcttggca gaacaacgcc tcttccaaca cgacctttat ccagagaaaa ttgacaaccg
    24121 ctaaagtcat actcaatgaa aatcaaagag caaactagga agttagccgc aggttactca
    24181 aagcagtgtt tgaggttgta gataaaactg acgaagtcag ct
//
LOCUS       DAVBDG010000030        24005 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBDG010000030 DAVBDG010000000
VERSION     DAVBDG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.030"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(255..2513)
                     /locus_tag="VQ526_001175"
     CDS             complement(255..2513)
                     /locus_tag="VQ526_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2554902.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2742..2972)
                     /locus_tag="VQ526_001176"
     CDS             complement(2742..2972)
                     /locus_tag="VQ526_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2554903.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3162..3845
                     /locus_tag="VQ526_001177"
     CDS             3162..3845
                     /locus_tag="VQ526_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2554904.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3845..4579
                     /locus_tag="VQ526_001178"
     CDS             3845..4579
                     /locus_tag="VQ526_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2554905.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVIENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4733..5590
                     /locus_tag="VQ526_001179"
     CDS             4733..5590
                     /locus_tag="VQ526_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2554906.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5732..6604
                     /locus_tag="VQ526_001180"
     CDS             5732..6604
                     /locus_tag="VQ526_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2554907.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQDRVSKDKLV"
     gene            6886..7569
                     /gene="rpiA"
                     /locus_tag="VQ526_001181"
     CDS             6886..7569
                     /gene="rpiA"
                     /locus_tag="VQ526_001181"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2554908.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7583..8794
                     /locus_tag="VQ526_001182"
     CDS             7583..8794
                     /locus_tag="VQ526_001182"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2554909.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8796..9338
                     /locus_tag="VQ526_001183"
                     /pseudo
     CDS             8796..9338
                     /locus_tag="VQ526_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            9354..10163
                     /locus_tag="VQ526_001184"
     CDS             9354..10163
                     /locus_tag="VQ526_001184"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2554910.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10639..11370
                     /locus_tag="VQ526_001185"
     CDS             10639..11370
                     /locus_tag="VQ526_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554911.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAEVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTP
                     LIHNDRDIVTFTGNSRFKHRSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRF
                     FKKQALPRSSSQPSAEPMENIALGK"
     gene            11416..11619
                     /locus_tag="VQ526_001186"
     CDS             11416..11619
                     /locus_tag="VQ526_001186"
                     /inference="COORDINATES: protein motif:HMM:NF012956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2554912.1"
                     /translation="MDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIRQINIYNRT
                     DTAQDRLANFDVILLDSSGKEIE"
     gene            11644..12156
                     /locus_tag="VQ526_001187"
     CDS             11644..12156
                     /locus_tag="VQ526_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2554913.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12814..13524
                     /gene="deoD"
                     /locus_tag="VQ526_001188"
     CDS             12814..13524
                     /gene="deoD"
                     /locus_tag="VQ526_001188"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2554914.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13547..14071)
                     /locus_tag="VQ526_001189"
     CDS             complement(13547..14071)
                     /locus_tag="VQ526_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2554915.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14124..14360)
                     /locus_tag="VQ526_001190"
     CDS             complement(14124..14360)
                     /locus_tag="VQ526_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2554916.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14428..15348)
                     /locus_tag="VQ526_001191"
     CDS             complement(14428..15348)
                     /locus_tag="VQ526_001191"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2554917.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15546..15950
                     /locus_tag="VQ526_001192"
     CDS             15546..15950
                     /locus_tag="VQ526_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554918.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGICFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16166..16747
                     /locus_tag="VQ526_001193"
     CDS             16166..16747
                     /locus_tag="VQ526_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2554919.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16744..18021
                     /locus_tag="VQ526_001194"
     CDS             16744..18021
                     /locus_tag="VQ526_001194"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2554920.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            18039..18701
                     /gene="deoC"
                     /locus_tag="VQ526_001195"
     CDS             18039..18701
                     /gene="deoC"
                     /locus_tag="VQ526_001195"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2554921.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18688..19077
                     /locus_tag="VQ526_001196"
     CDS             18688..19077
                     /locus_tag="VQ526_001196"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2554922.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19167..20219
                     /locus_tag="VQ526_001197"
     CDS             19167..20219
                     /locus_tag="VQ526_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2554923.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20363..21898
                     /locus_tag="VQ526_001198"
     CDS             20363..21898
                     /locus_tag="VQ526_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554924.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            21891..22949
                     /locus_tag="VQ526_001199"
     CDS             21891..22949
                     /locus_tag="VQ526_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554925.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22952..23908
                     /locus_tag="VQ526_001200"
     CDS             22952..23908
                     /locus_tag="VQ526_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554926.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6963 a         4338 c         5467 g         7237 t
ORIGIN      
        1 tgcaatacct tttacgaggc tcttttgtcc tgctcttgtt tcaattgact atagcagatt
       61 gaaataagat gagaacaaat cgattgggaa agtaaaatta atttctataa atgttttagc
      121 aattgtttcg tactatttca gattcagtct actatatatc ataatggaca aagggcaagc
      181 tgtcaagaaa ttacagttat tttagtaaaa aaagaagcag aaaaatttca ttttctactc
      241 cttttctata ggatttattt ttctttttca gaacttttaa cttcagcttt tttggcagat
      301 ttaatctgaa tgtttccctt gctagtcata actgctttga gatctttttc gcttggattt
      361 tcaaggtagt agtcagtgat tgtgtcctca atatagtctt gaatagtccg acgaagtggg
      421 cgtgctccca tttttggatc ataacctagg tcaaccaact tttccttgac cttgtcagtt
      481 acatccaaac gaatgttgtt gctagagagg cgcttgttaa catctgctag catgagctcg
      541 acaatctgaa ggaggttatc cttgctgaga gccttaaatt cgataatgcc atcaaaacgg
      601 ttcataaact ctgggctaaa gaagttaccg agttcaccga ggacagaatt ggtacgtcct
      661 tctctagcag caccaaatcc aacgctagct tcggtctttc ctgtacctgc atttgaggtc
      721 atgataatga tggcatcctt gaagctaacg gtgcgtcctt gcccgtctgt caaacgacca
      781 tcgtccaaga cttgaagaaa catgtgcata acatctgggt gagctttttc cacttcatcg
      841 agaaggatga gagaatatgg attgtggcga actttttcag ttaattgacc agcctcatca
      901 tagccaacat aacctggagg agcgccgacc aacttagcca cactatgttt ttccatgtat
      961 tcactcatat caaagcgaat catactatca gcagaaccaa aaagttcgat agccagttgt
     1021 ttggaaagtt ctgtcttacc gacaccagtt ggcccaacga agaggaagct tccgattggg
     1081 cggttagggg taccaagtcc gacacgatta cggcgaatag ccttggcaat cttatcgact
     1141 gcatcatctt ggccaataac atgagacttg agatcttcgg ctagatggat gagttgagat
     1201 tgttctttct ctttcaaatc accaacaggg atattggttt tctgctcgat aatgtgctca
     1261 atagttttct cgctgatgat aggagtatcc tggtctgtga tctttttctt ttgcatttcc
     1321 ttatacttgg caatctggtc gcggaagtag gccgccttct caaaatcttc ttctcgtgta
     1381 gcttgagact tgagattttc agcctcaatc aagcgctgat caattacttt aggatccaca
     1441 aaattcaagg tcaagttcat cttagaacca gcttcatcta ggaggtcaat ggccttgtca
     1501 ggcaagaagc gatcttggat gtagcgattg gaaagagttg cagctgcttc aatcgcagca
     1561 tcggtatatt gaacgtggtg gtaatcttcg tatttctttt gaatcccttt gagaatagtg
     1621 attgtttcat ccaccgttgg ttcatcgact ttaacaggct gcatacgacg ctcgagggca
     1681 gcatcctttt caatgatacg gtattcattg agggtagtag caccgactag ttgcagttct
     1741 ccacgagcaa gggctggctt gaggatattt cctgcgtcca tattaccatc actcgcagaa
     1801 ccagcaccaa caatttcatg gatttcatcg ataaagagga tgatgtcttc acgtttgcga
     1861 atttcttcca tgagtttttg catgcgttct tcaaattgtc ctcgaatccc cgttccttga
     1921 actaagctaa ccacatccag acggatgact tgtttacctt ggagtttatg tggcacatcg
     1981 ccatcgacaa ttttctgagc tagaccttcg acaacggccg tttttccgac accaggttca
     2041 ccgataagga caggattatt cttcgttcta cgattgagaa tctcgatgac acggataatc
     2101 tcatcgtcgc gcccaataac ggggtcaatg tctccacgac gggcaatttc agttacatta
     2161 ataccaaatt cttccagcag gcctttttct tggctaggtg gcggagtttg agcagatcca
     2221 cgattttggg aaccataacc gccgtttcca ccgtaacctc cacctgattg ggttggggga
     2281 ataggaggag tattgctaga aggtctgaaa ttgtttagat cattgaagaa atcaccaaag
     2341 ggatcgaagt cacgattgtt cagatccgtc atacctttga agaggctatt gttaggatct
     2401 gttttgataa tcttatagca gttttgacag aggtcaattt gtttttgttt tccattgaga
     2461 ttggtgtaaa gatgaattgt tgagtcgtta attttacagt tttgacaaag catacatcta
     2521 cctcatttct ttctttagcc tgacttgttt aaaggtcaaa aatagtcaaa ttctatactc
     2581 tcattataac aggattgggg tgtaaagcaa gtaaaaagat tgaagctttg agaagttgtt
     2641 acaatgaaaa tttttgtgaa tttggactat aaaaaaatcc tatttgtgag acaaaaagga
     2701 ttttacttag acatgcaggg acaatcccag cttgttttag attagtagag aagttcgtaa
     2761 atttccattg cgattaagtc aatgctatca aacgtatacg tttcgcgatc ttctacgtca
     2821 cgaagggtaa agattgggcc gttttcttcg tcgtggctgt acgcaacttc tgcaacaaca
     2881 actccttcgc gttcaaaatt acgttttaaa tttccgccat cgtttgccat tgcttcaagg
     2941 cggttgatga ttctaaccaa atgtgattcc attttgattc tccttcattt cttatcgtta
     3001 ctattttacc ataaaggagg ccaacttgac tagaaaaaac taagatttct cgctaattct
     3061 gctagtttaa agatttttaa ataaattgga taaaaaattt aaattttaat tcaaaacatg
     3121 ttataatcat acagtattct attttagaaa gcagtgtgac tatgaatttt tcttttttac
     3181 ctaagtattt accttatttt aactatgggg ctgttgtgac gattcttatt tctatctgtg
     3241 ttatcttttt gggaactatt ttgggtgttg tcttggcttt tgggcaacgt tcaaagttta
     3301 aaccgcttgt ttggttggcc aacttgtacg tttggatttt ccgtgggaca ccgatgatgg
     3361 ttcaaattat gattgccttt gctcttatgc atatcaatgc tccgactatt cagattggaa
     3421 ttttaggtgt tgatttttcg cgtctgattc cagggatttt gattatctct atgaatagtg
     3481 gtgcttatgt ttcggagact gttcgtgccg gaatcaatgc ggttccaaaa ggtcagctag
     3541 aggcggctta ttcgctaggg attcgtccta aaaatgcgat gcgttatgtg attttgccac
     3601 aagcaatcaa aaatatcttg ccagcattgg ggaacgaatt tatcaccatt atcaaggaca
     3661 gctccctctt atcagctatt ggggtcatgg agttgtggaa tggggctaca acagtttcta
     3721 caacaaccta tctaccttta acaccgcttt tatttgcagc attttattac ttgattatga
     3781 cctctattct gacagtagcc ttgaaagctt ttgaaaaaca tatgggacaa ggagataaga
     3841 aataatgaca gaaaccttga taaaaattga aaatttacat aaatcttttg gaaagaatga
     3901 agtattgaag ggaatcaacc tcgagattaa aagaggagaa gttgtcgtta tcatcggtcc
     3961 ttcagggagt gggaaatcta ccttgcttcg ctctatgaat ttgttggagg aagcaaccaa
     4021 ggggaaggtt atctttgagg gagtcgatat tacggacaag aagaatgact tgtttgccat
     4081 gcgtgagaag atgggcatgg ttttccaaca atttaacctc tttcctaata tgactgtgat
     4141 tgaaaatatt accttgtcac ctatcaagac caaaggtgaa agcagggaag ttgctgagaa
     4201 gagagcccaa gagcttttgg aaaaagttgg tttgccagat aaggcagacg cttatccaca
     4261 gagtctgtcg ggtgggcagc aacaacggat tgccatcgcg cgtgggttgg ctatggaacc
     4321 agatgttttg ctctttgacg aaccaacttc agccttggat cctgaaatgg taggtgaggt
     4381 attggctgtt atgcaagacc ttgccaagtc agggatgact atggttatcg taacacatga
     4441 gatgggattt gcccgtgagg tggcagatcg tgttatcttt atggcagacg gtgtggttgt
     4501 tgaagatggg acacctgagc agatttttga acaaacccaa gaacaacgga ctaaagattt
     4561 cttgagtaag gttttataat ctattttaaa atttcaagac aagattagtg aaaagttcaa
     4621 ccagagcttt ttcttatagt ttgaaactat aggataacct aggaaagaag tgttagaggg
     4681 cactagcagt ttttggtata atggaagata tttgaaagaa aagagaagtg atatgacaca
     4741 gattattgat gggaaagctt tagcggccaa attgcagggg cagttggctg aaaagactgc
     4801 aaaattaaag gaagaaacag ctctagtgcc tggtttggta gtgattttgg ttggggataa
     4861 tccagccagc caagtctacg ttcgcaacaa ggagaggtca gctcttgcgg ctggttcccg
     4921 tagcgaagta gtgcgagttc cagagaccat tactcaagag gaattgttag acctgattgc
     4981 taaatataat caggatccag cttggcatgg gattttggtc cagttgccat taccaaaaca
     5041 tatcgatgaa gaggcggttt tattagccat tgacccagaa aaggatgtgg atggtttcca
     5101 tcccctaaac atgggacgtc tttggtctgg acatccagtc atgattccct cgacacctgc
     5161 aggaattatg gaaatgtttc atgaatatgg gattgacttg gaaggtaaaa atgcggtcgt
     5221 aatcggtcgt tcaaatatcg ttggaaaacc tatggctcag cttcttttgg ctaaaaatgc
     5281 gacagtaacc ttggcccact cacgaactca taatcttgcc aaggtggctg ctaaagcaga
     5341 tattcttgta gtcgcaatcg gccgcgccaa gtttgtgact gctgactttg tcaaaccagg
     5401 tgcggttgtc attgacgttg ggatgaaccg agatgaaaat gggaagctct gtggagatgt
     5461 tgattatgag gcagttgcac cacttgctag ccatatcacg ccagtccctg gaggtgtcgg
     5521 tcctatgacc attaccatgc tgatggagca aacttatcaa gcagcacttc ggacattgga
     5581 tagaaaataa gagaaacatt cgattaatag agtgtatttt ctatagctat atctaaaatg
     5641 gcagaaatga atattaaatt ttagatataa gattacaaaa ggaggtctgc gtctcctttt
     5701 tgttgtataa taaaagtgag agggaaaaag gatgaaagtg attaatcaaa ccttactaga
     5761 aaaagttatt attgaacgtt ctcgttcaag tcataaagga gattatggtc gtctgctgct
     5821 gcttggtggt acttatcctt atggaggtgc catcatcatg gctgctttag cagctgtaaa
     5881 aagcggtgca ggattggtga ccgttggaac ggacagggaa aatatcccgg ctctgcacag
     5941 ccatttacct gagcctatgg ccttttctct tcaagaccag caattgttaa aagagcaatt
     6001 ggagaaggca gaagttatct tgctggggcc tggtttacga gacgatgctt ctggagaaaa
     6061 tctagtaaaa caggtctttg ttaatttaag ccaaaatcag attttgattg tagacggagg
     6121 tgccttgacc attcttgcta ggacaagttt gtcatttccc tcaagccagc ttatcctaac
     6181 tcctcaccaa aaagaatggg aaaaattatc tggaattacg attgaaaagc aaaaggaaga
     6241 tgcaacggct agtgttctga cttccttccc tcaaggaaca attttggtgg agaaaggccc
     6301 agctactcgt atttgggaag ttggccaatc tgattattac cagttacagg ttggtggtcc
     6361 ttatcaggcg actgggggaa tgggagatac tctggctggg atgatcgcag gatttgtagg
     6421 ccaattccga caggccagtc tctacgagcg tgtggcagta gcgacccatc ttcattcagc
     6481 catagcccaa gaactatctc aagaaaatta tgtggtctta ccgacggaaa tcagcaggta
     6541 tcttcccaaa ataatgaaaa ttatttgtca acaagatagg gtaagcaaag ataaacttgt
     6601 ttaaaataga cagataaatc tacttccttc tcttaattta tgattcttga aatgcgcctg
     6661 ctttaaggct aaactagacg tatcaaagtt aagagtttgt ttttgtaact ggaatgagat
     6721 gaacaaatca aatacataaa agataaactc atttttattc gtttggtaaa aataaaatgc
     6781 atatttttaa agaaagattg ataaaaaatg tagatttgat gtttttactt gaataaaaag
     6841 cggtttcata gtaaaataag aactgagaat gatggaggta aatcggtgga aaatctgaag
     6901 aaaatggcag gtatcaaggc tgctgagttc gtgagcgatg gaatggtcgt tggacttgga
     6961 acaggctcga ctgcctatta ttttgtcgaa gaaatcggtc gtcgtatcaa ggaagaagga
     7021 ttgcagatta cagctgtaac aacttctagt gtgaccacta aacaggcaga aggccttaat
     7081 atcccgctca agtctattga ccaagtagac tttgtcgatg tgacggttga tggcgcggat
     7141 gaagtggata gtcagtttaa tggaatcaaa ggcggtggtg gtgcccttct catggaaaag
     7201 gtggtcgcaa caccatcaaa agaatacatt tgggtggtgg atgaaagcaa gctggtcgaa
     7261 aaactaggtg cttttaaatt gccagtagaa gtggttcagt atggtgcaga gcaggtcttt
     7321 cgtcattttg aacgagctgg ctacaaacca agtttccgtg aaaaagacgg ccaacgtttt
     7381 gtgacggata tgcagaattt tatcattgac ctcgccttgg atgtcattga aaatccaatt
     7441 gcttttggac aagaattgga ccatgtcgtt ggtgttgtgg agcatggttt attcaaccaa
     7501 atggtggata aggtaatcgt tgctggacga gatggagttc agatttcaac ttcaaaaaaa
     7561 ggaaaataga agggggcata agatgtctaa atttaatcgt attcatttgg tggtactgga
     7621 ttctgtagga atcggtgcag caccagatgc taataacttt gtcaatgcag gggttccaga
     7681 tggagcttct gacacactgg gacacatttc aaaaacagtt ggtttgaatg tcccaaacat
     7741 ggctaaaata ggtcttggaa atattcctcg tgaaactcct cttaagactg tagcagctga
     7801 aagcaatcca actggatatg caacaaaatt agaggaagta tctcttggta aggatactat
     7861 gactggacac tgggaaatta tgggactcaa cattactgag cctttcgata ctttctggaa
     7921 cggattccca gaagaaatcc tgacaaaaat cgaagaattc tcaggacgca aggttattcg
     7981 tgaagccaac aaaccttatt caggaacggc tgttatcgat gattttggac cacgtcagat
     8041 ggaaactgga gagttgatta tctatacttc agctgaccct gttttgcaga ttgctgccca
     8101 cgaagacatt attcctttgg atgaattgta ccgtatctgt gaatacgctc gttcgattac
     8161 ccttgagcgt cctgcccttc ttggtcgcat cattgctcgc ccttatgtag gtgaaccagg
     8221 taacttcact cgtacggcaa accgtcgtga cttggctgta tctccatttt ccccaactgt
     8281 tttggataaa ttgaatgagg ctggtatcga tacttatgct gtgggtaaaa tcaacgatat
     8341 ctttaacggt gctggtatca accatgacat gggtcacaac aagtcaaata gtcatggaat
     8401 tgatacacta ttgaagacta tgggacttgc tgagtttgaa aaaggattct cattcacaaa
     8461 cctagttgac tttgatgccc tttacggcca tcgtcgtaat gctcacggtt accgtgattg
     8521 cttgcatgag tttgatgaac gcttacctga aattatcgca gctatgagag agaatgacct
     8581 tctcttgatt actgcggacc atggaaatga cccaacgtat gcaggaacgg atcacactcg
     8641 ggaatatatt ccattgttgg cctatagccc tgcctttaaa ggaaatggtc tcattccagt
     8701 aggacatttt gcagatattt cagcgactgt tgccgataac tttggtgtgg aaactgctat
     8761 gattggggaa agtttcttag ataaattggt ataagatgac gcgctatgct ttgctggtga
     8821 gaggtatcaa tgttggtggt aagaataagg tcgtcatggc ggaacttcgt caagaattga
     8881 caaacttggg actggaaaag gttgagagct acatcaacag tggcaatatt ttctttactt
     8941 cgatagattc caaagcccaa ttggttgaaa agctagagac tttctttgca gtccattatc
     9001 catttattca gagcttttct ttactgagtc tagaggactt tgaggcggaa cttgaaaatc
     9061 taccagcttg gtggagcaga gacttggcac gaaaagattt tctcttttac actgagggtt
     9121 tggatgtgga ccaagtcatc gcgacagttg aaagtttaga gctgaaagat gaagtgcttt
     9181 attttggaaa acttgggatt ttctagggga aattttctga agaatcctat tctaagactg
     9241 cctatcataa gtacttgctg aaggtgcctt tctaccgcca cattactatt cgtaatgcta
     9301 aaacctttga caaaattggt caaatgctaa aaaaataata aaggagacac acaatgacat
     9361 ttttaaacaa aatccatgaa actgctactt tcctgaaaga aaagggaatt gcagcccctg
     9421 agttcggtct aatccttgga tcaggacttg gagaattggc agaagaaatc gaaaatccag
     9481 ttgtagtaga ctatgctgag attccaaact ggggccgttc aacagtagtc ggtcatgctg
     9541 gtaaattggt atatggtgaa ctggcaggtc gcaaggtctt ggctcttcaa ggtcgtttcc
     9601 atttctatga agggaatcct ctggaagtgg tgactttccc agttcgtgtg atgaaagttc
     9661 ttggatgtga aggtgttatt gtaaccaatg cagctggcgg tatcggattt ggtcctggta
     9721 ccttgatggc tatctcagac catatcaaca tgacggggca aaatccattg atgggtgaaa
     9781 acttggatga ctttggccca cgtttcccag atatgtctag ggcctacaca ccagaatacc
     9841 gtgccactgc ccatgaagtg gctaaaaaac ttaatatcaa gcttgatgaa ggtgtctata
     9901 tcggagttac tggtccgact tatgaaacac cagcagaaat tcgttcctat aagacactgg
     9961 gagcagatgc agttggtatg tctacggttc ctgaagttat cgtggcagcc cactctggct
    10021 tgaaagttct gggaatttca tgtatcacta actttgcggc tggtttccaa gaagaactca
    10081 atcacgaaga agttgtagaa gtgactgaac gtgttaaagg tgatttcaaa ggcttgctta
    10141 aagcgattct tgctgaattg taagaaaaaa gatttaaaag ggggagtgcc tctgtctttt
    10201 caggattgac tgcctatccg tattaaagaa gaaacagagg aatactatga gcttcttcct
    10261 gctcttataa ctgaaagaag cggaagaata ggtatgtctg atctgatagc cagcattgtg
    10321 aaagacaaga ttctaggata ctagcattag cttcctagcc aagcagacta gtatgataag
    10381 gagagatgag aatgaattga ctttctgaat ttctcagtct tatcatatat agcacaatga
    10441 gatttcgctt gagtctgctt gtaaataaac gaaaagaaag ataagaaata atgaaaattg
    10501 gtcaacgaat tatgcgcttt ggcataaaaa attaagtatc ggagttgtat ctgttgtagt
    10561 cggctttgat ttctagctcc agctggaatt tcagccaatg aagtaaagca agatgtaaca
    10621 tctgaagtgg taataggtgt gctagattct aaggaggaat tgaaagagtc agaaaatgat
    10681 gctccaaaac tagaaactcc tcttagagag gagccaagac tagctcctca aacgcttccg
    10741 gaagcaagtg aagttcttga aaacaaaagg gaagagtcaa aagtagagat aacagagcca
    10801 gctcaagcgg atgatatccg caaggttgtt ggggaattag ccaaggatat aagtattact
    10861 aagttgtata tgacaggtca ttctcttgga tgctacctag ctcagattgc agaggttgaa
    10921 gcttaccaaa aatatcctga tttttataac catgtattga ggaaagtgac aactttcagt
    10981 gctcctaaag tgattacttc cagaactgtt tggaatgcta agaatggttt ctgggatgtt
    11041 ggtttggaaa gtcgtaaatt agctgttagt ggaaaaatta agcattatgt ggttgataat
    11101 gacaatgttg tgactccctt gattcataat gatcgtgata ttgttacatt tacaggtaat
    11161 tcacgcttta aacaccgttc tcgtggctat tttgaaagtc caatgaatga tattcctaac
    11221 tttaatattg gtaaacaagc taccttggat aaacatggtt atcgtgatcc gaaattggat
    11281 aaagtgcgat tctttaagaa acaggctctg cctcgatctt ctagtcaacc aagcgctgaa
    11341 ccaatggaaa atattgcctt aggaaaatag gttactcaaa gttcgacagc tttcggagga
    11401 gatgctagaa gagctgtgga tggcaaagtc gatggtaact atggtcacaa ttctgtcact
    11461 catacaaact ttcaatctaa gccttggtgg caagtagatt tggctaaaga agaaaccatt
    11521 cgccaaatca atatttacaa ccgaacagac actgcccagg atagattggc aaactttgat
    11581 gtcattcttt tagacagttc tggtaaagaa attgagtgaa aacgtataac atctcctaaa
    11641 gatgtgtcag cacaaattac gattaaccat aaaaaagcgc gctatgttcg gattgagcta
    11701 gaaggctata atgccctcag tcttgcagaa gttgaagttt tccgctttat agctacgaat
    11761 ggtgaaacgg cgatacaagt ttctaagtca gttcaaccaa tcagtcagac tcctgtgaag
    11821 gataaaacat tgacaattca acacagtgga gcttacattg cccgctactc cataacttgg
    11881 gaagaagtta cagtagataa agatggaaac caagttgttc gtagtcattc ttgggaagga
    11941 aacggtcgca accagactgc aggttttgtc ctcaacctcc caatcaaaga aaatatgaga
    12001 aatctgcgag ttaagattga gaaaaagacg ggcctactat ggaatagatg gcaaacaatc
    12061 tatgaaaaca gaccaatttt agctcaaccc caccgtaaaa ttacccattg gggtacgaca
    12121 ttgaattcca aggtgagtga cgatgatgtc ttgtaatctg atggtacaat gatagttagt
    12181 ttgtctagtt tataagaaag tactacctga gcttgaatag gactcaggta gctctctgtg
    12241 aaagaacaaa attaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt
    12301 tgctcaaagc actgctttga ggttgtagat aagactgacg aagtcagtca catatataat
    12361 ccaaggcgac gttgacgtgg tttgaagaga ttttcgaaga gtataaacag aaaggtagag
    12421 cgcgtgttct aatttgaaca cgagtagaaa acttttctaa aaataaaaac gaaaggatgg
    12481 gtaaactgta ttcgctgaac tgaatacggg cgactctcct ctaaatcaaa attaagaaag
    12541 aaaggaattg accccaccct aaaagcagtg ggaaaaagat agttggtcta gcgagcatcg
    12601 ctcactgcgc ccaactccta ttttcccttc gctttttgat gggtttggta tctttctcaa
    12661 tataaaatat aaaataaaga aaggtagagc gtgtgttttg atttgaacac gagcggaaaa
    12721 ctcggaaaat agataatctg actgaaaaat caggatttct cgtcaggttc ctaattttca
    12781 gtcgttttct tctcgctctt tgtatcataa attatgtcta tccatattgc tgctcagcag
    12841 ggtgaaattg ctgataaaat tcttcttcct ggggatcctc ttcgtgctaa gtttattgcg
    12901 gagaatttcc ttggtgatgc tgtttgtttt aacgaagtgc gtaacatgtt tggttacact
    12961 ggtacttaca agggtcaccg tgtatctgtc atgggaactg ggatgggaat gccatctatt
    13021 tcgatttatg cgcgtgagtt aatcgtagac tacggtgtga agaaattgat tcgtgtggga
    13081 actgcaggtt ctttgaatga agaggttcat gttcgtgaat tagttttggc gcaggcggct
    13141 gcaaccaact caaacatcgt tcgtaatgac tggccacagt acgattttcc acaaattgct
    13201 agctttgatt tgcttgataa agcctaccat atcgccaaag aacttggtat gactactcac
    13261 gttgggaacg ttttgtcatc tgatgtcttt tactcaaatt actttgaaaa gaatatcgag
    13321 cttggtaaat ggggagtcaa ggctgtggaa atggaagcag cagctcttta ctatcttgct
    13381 gcccaatacc atgttgatgc gctagctatc atgaccatct ctgatagctt ggtcaatcca
    13441 gacgaagaca caactgcaga agaacgtcaa aataccttca ctgatatgat gaaggttggt
    13501 ttggaaacct tgattgcaga ataattatag ccaaaaagga agccctttat gaattgtagg
    13561 acttcctttt cttatccaga aattgatcta gctctctctg atttcgaaga atagtgactt
    13621 tatgtgaata ttcttggcaa agtttttggt aattttcttt ttgagttttg ctacgcccat
    13681 cccaaagaat ccatctgata aactcccagt caaagcgttc agggcaatct gccgccatac
    13741 tttctctgac ttttccacgg tatttaagat aacgcttaaa ggctctaaag agacaggtca
    13801 atggcaaaaa attgagaaag atgatttggt cagcttcttg cattcgttct tggtagtagc
    13861 accaagaata attaccatcg atgacccaag ctttatgctt ggtgagaaag ttttttatct
    13921 cggttaacat ccattcgcag tcactgtctt gccaaccagg ttgaaattgg agtgtgtcca
    13981 tgtgcagttt tggaatggag tagtagttag ataacttttc tgctagagtt gacttaccag
    14041 aaccagaata tccgataatt gtgattttca ttttctacct tttcctattt ggagacaaaa
    14101 aaacagcctc tatggactgt ttcttattta gcaagtttag ctgaaagacg agctttatcg
    14161 cggcttgctt tgtttttgtg aatcaaacct ttagtttctg ctttatcgat agctgagcta
    14221 gcagcacgga aaagttcttc agatgggttt gcttcgaaag cttttatagc agtacgcata
    14281 gctgattttt gagctgagtt cttttcgttt tgtttaacgt tcaattcagc gcgtttgata
    14341 gctgatttaa tgtttgccaa tgttcttacc tccatattta ctaactatac cattatatat
    14401 gaaaacttac gttttgacaa gggaaaatta tttttttaag taaatttcat cgatttcatg
    14461 gttcttgctt ttatgaagaa tcacttctgc acgatttctg gttggttcaa tataattttg
    14521 tagatttgtg agattgatac tggtccagac ctgatgggca aaggcttcca cttccccaat
    14581 cggcatctga gtaaaacgat aatagtagct atcagggtcg ttttgggcta ggctcagcat
    14641 tttcaagaaa cggtccagat accaactttc aatatcatcc actccagcat ctacatagat
    14701 ggaaaagtca aagaagtcag tgatatagag acgatcgttt tgtggatttt gaaagacatt
    14761 gattccctca acaattacaa aatcagcagc tttgacactt tgtttctctt cgggtacgat
    14821 gtcgtaaact tcatgagaat agacaggaat atctacatct tgtccatttt tgatgcggtc
    14881 caagaagttg agaagagctt ccatatcata gctttcagga aatcctttac gatttaaaat
    14941 cccctgctca atcaaggttt gattgggata gagaaaacca tcagttgtaa ccaactcaac
    15001 cgtagcatct gtaaacgtac gggacagtag gatttgaagt aggcgactgg ttgtggattt
    15061 tccaacggca acactcccag aaaccccaat aataaaaggt tgagatttac tttcacgttg
    15121 gaggaaaatt ccttttgaaa aggctaaatc ttccttagtt cgcttgtaaa tctgaatcag
    15181 atgagccaag gggagataga tatctgtaac gtcttggaga ctgatttggt cattaaaact
    15241 cttgatagat tccaattctt cttctgtcaa aggaggtgtt gtctttcgat gtaaagattg
    15301 ccaagtctgg cggctgattt tttcaaaatg taaaaattcg ttggtcattt gttttcccct
    15361 agatattttg tctatcatac cataaaaatc taaaaaaata aagcggtttc ttgaatgtgg
    15421 taagtcaatg gtatatagta taagtgatca atttattgat gaaataggag atagattcta
    15481 ctgatttata agtttataag cttataaagg gggcttttta gttcacaact tataggaggt
    15541 gtactatgaa aatcttaaaa cgttacatat tggaactctg ttttatttta agttttgctt
    15601 taccttttat aaaaggagcc aatgcagata atggtatatg ctttgtggaa acctattacg
    15661 gttttacttt tttgatggaa catgctattg taacagctgt ctttatctat tcgtttttaa
    15721 ttgctttctt actaaaaaaa cgatgggcga aatggattgc tgctggtagt tattgctttt
    15781 tagtcctatg gattgcgaca gagggttatt tcttccgcat gtcactagaa gatttgatac
    15841 gactttggac aagtttggaa atcctgacac aaacgtatca gttgggcttt tatctaaaca
    15901 ttttgttagg aattttgttg ataataaagt attttaaggt taagcaatag aaataataat
    15961 ctgcctgatt atagatgata aactttttgt ttttatctgg actgtgatat ttatagttct
    16021 aaatggttta ttatttataa gtgttgaaag agattgaaga aaatggcttc gtgacttgct
    16081 ttcttcggtc tttttcgttt tattatcaat ttgtaaacga tttacaattt gagtcaaatt
    16141 atggtaaaat agtgtcatga gtaaaatgta ttatgcagaa aatcctgacg ctgctcacga
    16201 cattcatgag ttgagagtag agttgttggg acaaaaaatg acctttttaa cggatgcggg
    16261 tgtttttagc aagaaaatag ttgactttgg aagtcaacta ttgctcaagt gtcttgaggt
    16321 caaccaagga gaaactgtcc ttgatgtagg ctgtggttac ggacctttgg gtttgtcctt
    16381 agctaaggct tatggagttc aggctactat ggttgatatc aacactcgtg ccttggattt
    16441 agctcggaga aatgctgaaa aaaataatgc aaaagcgacg attttccagt ccaacatcta
    16501 tgaacaagtt gaaggacatt ttgatcatgt catttccaat cctcctatcc gtgcgggcaa
    16561 gcaagtcgtt catgaaatca ttgagaaaag taaagatttc ttggaaactg gtggggattt
    16621 gacaatcgtc atccagaaaa aacaaggggc tccaagtgct aagtccaaga tggaggatgt
    16681 gtttggtaat tgtgaaatcc ttaaaaaaga caagggatac tatatcctta gaagtgtgaa
    16741 agaatgagag cagttgattt aatacaaaaa aaacgagacg gtcaagaact gacttcaagt
    16801 gaaattgaat ggctggtaga aggctatgtg tcaggaactg ttcctgatta tcagatgtct
    16861 gcctttgcta tggctgttta ttttaaagga atgacgactc gtgaaatttc tgatttaacg
    16921 atgaatatgg ttaagactgg gcaagagttt gacctgtcag ccattgatgg tgtgaaagtt
    16981 gacaagcatt ctactggtgg tgtcggcgat aaggtaacct tgattctagc tcctcttgtt
    17041 gctagcttcg gtgtgcctgt agcaaaaatg agtggtcgtg gtcttggcca tacaggcgga
    17101 acaattgata aattggagtc cattaagggc tatcaagtag aacgtagtca agaggatttt
    17161 attcgtcagg ttcaggatat tggagtatct gtcattgggc aatcagacca gctggtcaaa
    17221 gcagataagc ttctctatgc ccttcgtgat gtgaccgcaa ctgtcgacac gattcctttg
    17281 attgcgagtt cggtgatgag caagaaaatt gcggcagggg cggatgctat tttgctagac
    17341 gtgactgtcg gtgagggtgc cttcatgaag acggttgatg aggcgcgtga gttggctcaa
    17401 actatggtag atcttggtaa ggttgttgga cggaagacag tagcagtgat tacggatatg
    17461 agccagcctt tgggacgagc gattggaaat cgtctggaaa tccttgaagc attggagatt
    17521 ttgcaaggtc aaggacgtca ggatattacc cactttattt gtgaattggc tcaaattatg
    17581 cttggtttgg caaatgttaa caagactgtt gaagaagttc gccaacatct tgagaatggt
    17641 caagcactgg ctaagtttga ggaaatggtg caagcccaag gtggagactt ggaagacctc
    17701 tatcgtcctg taaatgtagc tcatgtggtg gaaatccctg ctcaggaaac gggtgtcatt
    17761 tcagctcttc cagctatgga ctttggtctt tatgccatga ggctgggtgc tggtcgtgca
    17821 gttaagtctg atgccttgga ctatgaaaca ggaattgttt ttgaaaagaa gattggagac
    17881 tccgttcaaa agggtgaaat tgttgcaaaa gtatacacaa atgaaaaaat tccacctcaa
    17941 ctagttacag atttccaaaa atgtgttaaa ataagtgatg aagtgaaaaa aatccgtgaa
    18001 attgtagaaa tcatctccta aatggaggga aaaccgaaat gaaattaaat aaatatatag
    18061 atcatacgct tttaaaacaa gatgcaaaga aaaaacaaat tgatagtttg ttgtctgagg
    18121 ctagagagta tggctttgcc agtgtttgcg ttaatccgac ctgggttgaa catgctaaaa
    18181 aaggacttga aggcacagat gttaaggttt gcacagtagt aggtttcccc ttgggagcaa
    18241 caacttcaac tgtgaaagct tttgaaacaa aagaagctat ccaaaatggg gctgatgaga
    18301 ttgacatggt aatcaatgtt ggggctctca agtcaggtga tttggccttg gttgagtcgg
    18361 atattcgtgc agtagtggaa gcaagtgggg acaagttagt gaaagttatt attgaagctt
    18421 gccttctgac agaccaagaa aaagttttgg cttgccaatt agcccaaaaa gcaggagctg
    18481 actttgtcaa aacatctact ggcttttcaa ctggtggtgc tacgatagca gatgttagat
    18541 taatgcgtga aacagttgga cctgatatgg gtgtcaaggc cgccggtgga gctcgttctt
    18601 atgcagatgc tcttaccttt gtcgaagcag gtgcgacccg tatcggaacg tcagctgggg
    18661 tagctatttt aaaaggagaa ttggcagatg gcgactactg agttgattga actggcaatt
    18721 gaaaccagca aacatgccta tgtcccctat tctcactttc ctatcggagc agttttagta
    18781 gccaaagacg gaagtgttta cacgggagtg aatatcgaaa atgctagcta tcctttgacc
    18841 aattgcggtg agagaacagc tatttttaag gctatatctg aaggccaaag agaattttca
    18901 gaattgattg tctatggtca aactgaaaaa ccgatctcgc catgtggtgc ttgtcgccaa
    18961 gtgatggctg aattttttga acaagatcta aaagtgacct tagtcgcaaa agataaatcg
    19021 acggtcgaga tgacggtcgg ggaattactt ccatactctt ttacagactt aaactagtct
    19081 gagtcgctct ctgagtggca cggtccttgt ggccaaccaa tccatacttg caacatcgtt
    19141 gcacatctta tttaggaggt tcagtaatga acaagaaaca atggctaggt cttggcctag
    19201 ttgcagtggc agcagttgga cttgctgcat gtggtaaccg ctcttctcgt aacgcagctt
    19261 catcttctga tgtgaagaca aaagcagcaa tcgtcactga tactggtggt gttgatgaca
    19321 aatcattcaa ccaatcagct tgggaaggtt tgcaggcttg gggtaaagaa cacaatcttt
    19381 caaaagataa cggtttcact tacttccaat caacaagtga agctgactac gctaacaact
    19441 tgcaacaagc ggctggaagt tacaacctaa tcttcggtgt tggttttgcc cttcacaatg
    19501 cagttgaaga agcagctaaa gaacacactg acttgaacta tgtcttgatt gatgatgtga
    19561 ttaaagacca aaagaatgtt gcgagcgtaa ctttcgctga taatgagtca ggttaccttg
    19621 caggtgtggc tgcagcaaaa acaactaaga caaaacaagt tggttttgta ggtggtatcg
    19681 aatctgaagt tatctctcgt tttgaagcag gattcaaggc tggtgttgcg tcagtagacc
    19741 catctatcaa agtccaagtt gactacgctg gttcatttgg tgatgcggct aaaggtaaaa
    19801 caattgcagc cgcacaatac gcagccggtg cagatattgt ttaccaagta gctggtggta
    19861 caggtgcagg tgtctttgca gaggcaaaat ctctcaacga aagccgtcct gaaaatgaaa
    19921 aagtttgggt tatcggtgtt gatcgtgacc aagaagcaga aggtaaatac acttctaaag
    19981 atggcaaaga atcaaacttt gttcttgtat ctactttgaa acaagttggt acaactgtaa
    20041 aagatatttc taacaaggca gaaaaaggag aattccctgg cggtcaagtg atcgtttact
    20101 cattgaagga taaaggggtt gacttggcag taacaaacct ttcagaagaa ggtaaaaaag
    20161 ctgtcgaaga tgcaaaagct aaaatccttg atggaagcgt aaaagttcct gaaaaataat
    20221 ggatggaagt catttcttag gaagcggcca agggccgctt ttttaagagt tgagacaaag
    20281 tcattttgtc tcagtaaagc ttgctactag acaaactagc aagttttatt gaaataaaat
    20341 aagactctga aaggaagagc acatggcaca cgaaaatgtc attgagatgc gtgatattac
    20401 caaggtgttt ggtggatttg ttgccaacga caaaatcaac ttgcacctac gaaaaggtga
    20461 aattcatgca cttttaggag aaaatggggc tggtaagtcc acgctaatga acatgttagc
    20521 agggcttctt gaaccaacta gtggtgaaat cgcggtcaac ggtcaagttg tcaatctcga
    20581 ctccccatct aaagcagcta gcttgggaat cgggatggtt caccagcact ttatgttggt
    20641 tgaagccttc acagtggctg aaaacatcat tttaggtagt gaattgacta aaaatggtgt
    20701 gctagatatc gctggagcta gcaaagaaat caaggctctt tctgaacgtt atggcttagc
    20761 tgttgaccct tctgccaagg tagcagatat ctcagttgga gcccaacaac gtgtagaaat
    20821 tttaaaaaca ctttatcggg gggctgatat ccttatcttt gacgaaccaa cggctgtttt
    20881 gactccatca gaaattgatg agttgatggc tattatgaaa aatcttgtca aagaaggaaa
    20941 atcaattatc ttgattaccc acaaattgga tgaaattcgc gcagtttctg accgtgttac
    21001 agttatccgt cgtgggaaat caattgaaac cgttgaaatt gcaggggcta ccaatgctga
    21061 tttggcggaa atgatggtag ggcgttctgt ttcctttaaa acagagaagc aagcctctaa
    21121 accaaaagaa gtggttttgt ctatcaaaga tttggtggtc aatgaaaacc gtggtgttcc
    21181 agctgttaaa aatctgtcct tggatgttcg tgctggagag attgttggta ttgcggggat
    21241 tgatggaaat ggtcagtctg aactgattca agccattaca ggtcttcgta aggttgaatc
    21301 tggtagcatt gagctaaaag gagattcaat tgtaggcttg cacccacgtc agattacaga
    21361 actaagtgtt gggcacgttc cagaagaccg tcaccgtgat ggcttgattt tggaaatgat
    21421 gatatctgaa aatattgccc ttcaaaccta ctataaagaa ccacatagta aaaatggaat
    21481 tttgaattat tcaaatatta cttcttatgc taaaaagctg atggaagagt ttgatgttcg
    21541 cgctgccagt gaattagttc ctgcagctgc actctcagga ggaaatcaac aaaaagcaat
    21601 tattgctcgt gaaattgatc gagatcctga tctccttatc gttagccagc caactcgtgg
    21661 gttggatgtc ggtgccattg agtatatcca caaacgcttg attgaagagc gtgataatgg
    21721 caaggctgtc cttgttgtca gctttgaatt ggatgagatt ttaaacgtct cagaccgtat
    21781 tgccgttatc cacgatggta agattcaagg tattgtatca ccagaaacaa ccaataaaca
    21841 agaacttggt gtcttgatgg ctggtggaaa cttgggaaag gagaagagtg atgtctaaaa
    21901 aattacaaca aatttcggtt cccttgattt ctgtatttct aggaatttta ctcggagcca
    21961 ttgtcatgtg gatcttcggt tatgatgcta tttggggcta cgaagaattg ttctatacag
    22021 cctttggcag tctgcgtggg attggagaaa tcttccgtgc tatgggtcct ctggtcttga
    22081 ttggtcttgg ttttgcggtt gccagtcgag ctggtttctt taacgtcgga cttcctggtc
    22141 aggctttggc aggttggatt ctcagtggtt ggtttgcgct gtcgcatcca gatatgcccc
    22201 gtcccttgat gattctagca accatcgtga ttgccttgat tgctggtggg attgtcggag
    22261 cgattccagg tattcttagg gcctatctag ggacgtcaga ggttattgta accatcatga
    22321 tgaactacat tgtcttgtat gtagggaatg cctttatcca tgctttccct aaagacttca
    22381 tgcaaagtac agattcgacc attcgtgttg gggctaatgc aacttatcag acaccttggt
    22441 tggctgagtt gactggtaac tcacggatga atattggtat tttctttgcc atcattgccg
    22501 ttgcagttat ttggttcatg ctcaagaaaa caactcttgg ttttgaaatc cgtgcagttg
    22561 gtcttaatcc acatgcttca gaatatgctg gtatttctgc caagcggact attatcctat
    22621 ctatgattat ttcaggtgcc ttggcaggtc ttggtggagc tgttgaaggt ttgggaacct
    22681 tccagaacgt ctatgttcaa ggttcgtcat tagctatcgg atttaacgga atggcggtta
    22741 gtttgcttgc ggccaactca ccaattggta tactctttgc agccttccta tttggcgttc
    22801 tccaagttgg ggctcctggt atgaatgcgg cgcaggtacc atctgagctt gtcagcattg
    22861 taacagcgtc tattatcttc tttgtcagtg ttcattacct tatcgaacgc tttgtcaaac
    22921 cgaaaaaaca agttaaagga ggtaagtaaa gatgtctatt ataaccttgc tccccctctt
    22981 ggtgtcttct atgctgattt actcagcacc tctcatcttt acaagtatcg gtggtgtttt
    23041 ctctgaacgt ggtggtgtgg taaacgttgg ccttgaagga attatggtta tgggtgcctt
    23101 ttctggagtt gtctttaacc ttgaatttgc agaacaattt ggagcagcaa ctccatggct
    23161 atccttgctt gtagcaggat tggttggtag tgttttctct atcatccacg cagcagcgac
    23221 ggttcatttc cgtgcagacc atgttgtcag cggtacggta ttgaacttga tggcgcctgc
    23281 cttggctgtt ttcttagtta aagttcttta taacaaagga caaaccgaca acctaagtca
    23341 aacttttgga cgctttgatt tcccagtctt ggcaaatatc ccagtgattg gtgatatctt
    23401 cttcaagtca actagtctac ttggttatct ggcgattgcc ttctcattcc ttgcttggtt
    23461 tattctcttt aagactcaat ttggtcttcg tctccgctct gtcggtgaac atcctcaagc
    23521 agcggatacc ttgggaatca acgtctacaa gatgagatat ttagggatta ttatttcagg
    23581 ttttctaggt ggaattggcg gagcgattta tgcgcaatca atctcagtta acttctcagt
    23641 gacaactatt gttggacctg gatttatcgc ccttgctgcg atgatctttg ggaaatggaa
    23701 tccaatcggt gctatgcttt ctagtctctt ctttggactt tcacaaagtt tggctgttat
    23761 cggttctcaa ttgccgttcc tacaaggagt gccagcggtt tatcttcaaa ttgcacctta
    23821 tgttttgaca attcttgtct tagcagcctt ctttggaaaa gcagtcgcac caaaagcaga
    23881 tggtatcaac tacatcaaat caaaataagc atacaaaaaa acgtcagtcc aatgactgaa
    23941 ctgcccccca aaagttagac agaaaaaatc taacttttgg ggtgttttta ttatgaaact
    24001 aactt
//
LOCUS       DAVBDG010000031        21088 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBDG010000031 DAVBDG010000000
VERSION     DAVBDG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.031"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(109..2106)
                     /gene="metG"
                     /locus_tag="VQ526_001201"
     CDS             complement(109..2106)
                     /gene="metG"
                     /locus_tag="VQ526_001201"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2554927.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEITYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2230..3489)
                     /locus_tag="VQ526_001202"
     CDS             complement(2230..3489)
                     /locus_tag="VQ526_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2554928.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3491..4192)
                     /locus_tag="VQ526_001203"
     CDS             complement(3491..4192)
                     /locus_tag="VQ526_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554929.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4176..5375)
                     /locus_tag="VQ526_001204"
     CDS             complement(4176..5375)
                     /locus_tag="VQ526_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2554930.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5640..6986
                     /gene="gor"
                     /locus_tag="VQ526_001205"
     CDS             5640..6986
                     /gene="gor"
                     /locus_tag="VQ526_001205"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2554931.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDSSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RINLPLHTHKVPAKLEKTAKGITIHFEDGTSHTASQVIWATGRRPNVKSLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFTSMYSACTCNRQETRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMRATKADFDATVAIHPTSSEEFVTMR"
     gene            7182..7718
                     /locus_tag="VQ526_001206"
     CDS             7182..7718
                     /locus_tag="VQ526_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2554932.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(7924..8139)
                     /locus_tag="VQ526_001207"
     CDS             complement(7924..8139)
                     /locus_tag="VQ526_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2554933.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8237..8557
                     /locus_tag="VQ526_001208"
     CDS             8237..8557
                     /locus_tag="VQ526_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554934.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8588..8926)
                     /locus_tag="VQ526_001209"
     CDS             complement(8588..8926)
                     /locus_tag="VQ526_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554935.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(8938..9657)
                     /gene="trmD"
                     /locus_tag="VQ526_001210"
     CDS             complement(8938..9657)
                     /gene="trmD"
                     /locus_tag="VQ526_001210"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2554936.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQVYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9647..10165)
                     /gene="rimM"
                     /locus_tag="VQ526_001211"
     CDS             complement(9647..10165)
                     /gene="rimM"
                     /locus_tag="VQ526_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2554937.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10196..10984)
                     /locus_tag="VQ526_001212"
     CDS             complement(10196..10984)
                     /locus_tag="VQ526_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2554938.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGRNHHYLQWSESEKIAAIIRELSE"
     gene            complement(11088..11331)
                     /locus_tag="VQ526_001213"
                     /pseudo
     CDS             complement(11088..11331)
                     /locus_tag="VQ526_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(11561..11800)
                     /locus_tag="VQ526_001214"
     CDS             complement(11561..11800)
                     /locus_tag="VQ526_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2554939.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(11820..12092)
                     /gene="rpsP"
                     /locus_tag="VQ526_001215"
     CDS             complement(11820..12092)
                     /gene="rpsP"
                     /locus_tag="VQ526_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2554940.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            12262..12471
                     /locus_tag="VQ526_001216"
     CDS             12262..12471
                     /locus_tag="VQ526_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554941.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            12950..13171
                     /locus_tag="VQ526_001217"
     CDS             12950..13171
                     /locus_tag="VQ526_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554942.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            13254..14057
                     /locus_tag="VQ526_001218"
     CDS             13254..14057
                     /locus_tag="VQ526_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2554943.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(14113..15654)
                     /locus_tag="VQ526_001219"
     CDS             complement(14113..15654)
                     /locus_tag="VQ526_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2554944.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(15656..16138)
                     /locus_tag="VQ526_001220"
     CDS             complement(15656..16138)
                     /locus_tag="VQ526_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2554945.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(16138..17223)
                     /gene="rlmN"
                     /locus_tag="VQ526_001221"
     CDS             complement(16138..17223)
                     /gene="rlmN"
                     /locus_tag="VQ526_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2554946.1"
                     /translation="MKPSIHSLTHQTIQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGT
                     DIDAACGQLLFNTMKRDRQKAVAAVNP"
     gene            complement(17244..17774)
                     /locus_tag="VQ526_001222"
     CDS             complement(17244..17774)
                     /locus_tag="VQ526_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2554947.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            complement(17930..18535)
                     /locus_tag="VQ526_001223"
     CDS             complement(17930..18535)
                     /locus_tag="VQ526_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2554948.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(18708..19745)
                     /locus_tag="VQ526_001224"
     CDS             complement(18708..19745)
                     /locus_tag="VQ526_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2554949.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(19779..20714)
                     /locus_tag="VQ526_001225"
     CDS             complement(19779..20714)
                     /locus_tag="VQ526_001225"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2554950.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
BASE COUNT         6167 a         4566 c         3873 g         6482 t
ORIGIN      
        1 tcaaagcagt gctttgatca acccgcggct agcttcctag tttgctcttt gattttcatt
       61 gagtataaat aaaaaaacag aactagtctg aactagtcct gtctactttt acccaatcac
      121 acttccattt ggtacagctg gatcaactgt gagaagggtt aatttgccat catgttcagc
      181 tgatagaatc ataccctggc tgacatattt tttcatcatt ttacgtggtt tgaggttagc
      241 aacgatttga actttcttgc cgaccaattc ttgttcattt ggatagtatt ttgcaattcc
      301 tgaaagaatc tgacgatctt ctccatcacc agcatccaag cggaattgaa gcaacttatc
      361 tgaaccttct actttagaca cttctttgac ttctgcgaca cggatttcaa ccttgtcaaa
      421 gtcttcaaac ttgatttcat ccttgtttag tttgagctca acttcgtccg gattccattc
      481 tttttcgact gctggtttat tgccttccat ttgttccttg atataggtga tttcttcttc
      541 catatttaga cgtggaaaga taggtgttcc tttggcaact acagtcacat ctgctgggaa
      601 gtcagccaaa ctcaagtttt caagactaga aacttcttcc aaaccaagtt gagtcaaaac
      661 tgcacgacta gtttccatca taaatggttc aatcaagtga gcaactacac gaatgctggc
      721 tgccaagtgg ctcatgacac ttgccaattg gtcacgaaga gcttcatcct tggccaagac
      781 ccatggtgcg gtctcatcga tgtatttatt ggtacgagag atcagagtcc agactgcttc
      841 aagcgcacgt ggatagtcaa ctgcttccat gtgtgtatgg aagtctgcga ttgattgttc
      901 tgcaacctca gcaagaacat gatcaaattc agtcacacct tctacatagg cagggatttg
      961 tccatcaaag tacttattaa tcatggaaac cgtacggtta aggaggttcc caaggtcatt
     1021 agccaattca tagttgatac ggccgacata gtcttcagga gtaaaggttc cgtctgaacc
     1081 aactggaagg ttacgcatga ggtagtaacg aagtggatct agtccataac gctctaccaa
     1141 catttcaggg taaacgacat tcccttttga cttagacatt tttccgtctt tcatgacaaa
     1201 ccaaccatgg gcaatcaaac gatcaggtaa tttaacatcc aacatcataa gaaggattgg
     1261 ccagtagata gagtggaagc gaaggatatc ttttcctacc atatggaaga ctgttccatt
     1321 ccagaacttg tcaaagttac catgttcgtc ttgagcgtag ccaagagctg tcgcatagtt
     1381 aagaagggca tcaatccaaa cgtagacaac gtgttttgga tttgatggga caggcactcc
     1441 ccatgtaaag gttgtacgag ataccgccaa atcttccaaa cctggctcga tgaagttgcg
     1501 tagcatttca ttaaggcgac catctggcgt gataaattca ggatgagctt tgaaaaattc
     1561 gaccaaacgg tcttggtatt tgctaaggcg aagaaagtat gattcttcag aaacccattc
     1621 aacctcatgg cctgatggag caataccacc agtcacattt ccagcttcat cacggaaaac
     1681 ttctgccagc tggctttctg taaagaattc ttcgtctgat actgaatacc aaccagagta
     1741 ttcacccaag tagatatcat cttgagcaag taagcgttca aagacttgtg cgacaacttt
     1801 ttcatggtag tcatcagttg tacggataaa tttatcgtat gagatatcta gtaattgcca
     1861 gagttcttta actccaaccg ccattccatc aacataggct tgaggtgtaa taccagcttc
     1921 ttccgctttc tgctggattt tctgaccatg ttcatcaaga cctgtcagat aaaatacatc
     1981 gtagcccatc aggcgtttgt aacgtgctag gacatcacat gcgatagttg tgtaggcaga
     2041 accgatatga agtttcccag atggatagta aatcggcgtt gtaatataaa aatttttttc
     2101 agacataatt tttcctttcc aggcaaatga aacctgtttt tctaacactt cattatatca
     2161 catttttaat gaatttcgat agggaaatcc atacaaaaac aagatagacg agtgtccatc
     2221 ttgttgatct cattcataac gaagggcttc aattggatca agtttcgatg ccttgttggc
     2281 tggcaagact ccaaaaatca taccaacact agccgaaact gcaagactaa atagggcaac
     2341 tgggattgat actccaactt ctataccttc tattaaacct tgcagtaaca aacctgctaa
     2401 ggcagttaaa ccacttgcaa ttgtcaagcc aattaagcca cctaacaagg tcaaaatcat
     2461 ggattcaatc aaaaactgaa ttaaaatatt ggcacgtgtt gcgcccaaag ccttacgaag
     2521 accaatctca cgagtgcgct ctgtcaccga aaccagcatg atgttcatga caccagttcc
     2581 tccaacaaag agagaaatcc ctgcgatgga actaataatc gtcgtcataa aactaaacga
     2641 ttgttgaatt tctgcaaata caacggactc atctgccacc tggtattctc cctgttgtaa
     2701 gcctgcaagc tctgtcattt ttcgtgccag ttctggaccc agagttgggg ttaaactggt
     2761 atcattcact cgaaagacaa tactagctat ttcatctata ttaaaattcg cagcaaggga
     2821 gatattggta gtaataggca agccaccaaa cccatatatt tttgaccttt tagcctccgg
     2881 actagtataa accccaatga cccggtaact aaatccattg acttctacaa ccttgttaat
     2941 agcctcttga ggagattcaa ataaactaat ggacaattcc tcatctagca aaataacact
     3001 tgcaaactct ttgaaatctt gctctctcag actacgacct gcaataattt cattcttaac
     3061 agcgtccatg taagttctgt ttccacctgt caaattagca ttctcaacct ttttatcttg
     3121 ataggtcaag atggcattcg ttgaattggt tacatagtaa ctatccactc ccttcagttt
     3181 agctgcctct tgaacccagg attcttgcgg ttttggcggt tcaacaggaa cttcctcttc
     3241 ctttccagaa accgtaaaag ctgattgttt ctgagtaaaa gacccatctt tacttttttt
     3301 aggagagaaa aagacgctaa tatttttctg agatttagtc atatctttat tgacttgacg
     3361 agatagggaa tcacccaaag ccataatcac aacaactgat gaaacaccga taataatccc
     3421 aatcatagta agcaaagaac gcatcttgtg agccatgata gatgaaaagg caaatttcag
     3481 attctgcatc ttagttttcc tcctttccta actgagcact gtcagacgaa atgaccccat
     3541 cccgaatgac aatctgacgt ttggcatagg cagcaatctc aggctcatgc gttaccatga
     3601 taatggtttt tccttcttta ttcaaatcaa ccaataattg cataatttgg ttacctgttt
     3661 tggtatccaa ggctcctgtc ggttcatccg ctaggataat agaaggattg tttaccaagg
     3721 cacgcgcaat ggctacacgt tgcttttgac caccagataa ttctgaaggt aaatggtgac
     3781 tacgttctgt caattcaacc ttgtctaaat attcctcagc caacttgcga cgttttgaag
     3841 acgaaactcc tgcgtaaatc aagggcaatt ctacattttg cagagcattg agcttcgata
     3901 gaagaaagaa ctgctgaaag acaaaaccga tttgttggtt acgaacctta gctagttgtt
     3961 tttcaccaag cccagccact tcttgacctt caagataata ttctccactg gttggtgtat
     4021 ccaacatgcc aatcgtattc atcagagtgg acttaccaga accagatggc cccatgatgg
     4081 ctacaaattc accctcattc acttctagat tgatattttt gagaacctgc agttcttggt
     4141 caccattacg gtagcttctg aagatatttt ttagactaat tagttgcttc atcagccttc
     4201 acctcttttc cttcttccaa ggaagatgtt ggattactga tgaccttagc accatttgtc
     4261 aaaccagaag tgatttcttg attttctgcg tcagcatttc ccaatgaaac ctcaactttt
     4321 ttagcctttt gttgttcatc cacaatccag acataatttt tactatcatc cattactaga
     4381 ctgctaacag gaacaagaat agccttagtt ttgcttttaa cctcaatgtt gacagaaaaa
     4441 ccttgtttca aatcaccaac ctcgcctgtc acatcaatag tataagggta tttagaacct
     4501 gtattattcc cggctgctgg actagctgct tcaccattgt ttttaggata gtcagaaata
     4561 tagcttaatt tcccagtcca ttttttatca ggatacactt tagaagtaaa gcttacttct
     4621 tgacctacag aaaggttggc tagattgtac tcagacaatt ctcccttgac ttgtaaattt
     4681 tcattgctga caatatgaac cataacttga ctcgcccctg ttggagattt agaaacattg
     4741 ctattgactt cgactacagt tccctctagg gtactgagaa cagttgttgc atccaattga
     4801 ctttgagcct tgcttaattg cgctgcagca tctgcacgcg catcacgggc atcacccaat
     4861 tgagcatcaa tagaagcaac agaatttcca gccactggag ttgggctttg caccgttgca
     4921 tcttctcctc ctactggcgc tggtaactgt ggagcctgag ctgaagcggc ttcatttcgt
     4981 gcttgattga gttcattgat atgacgatct gccttagcta ctgctcgact agctgaatca
     5041 taggccgcct gcgcttctga actactgtac ttgactaaag cctgcccttc gctgacctta
     5101 tcgcccacag aaacaaggat ttcatctaaa tcacccttac tagcatcaaa ataaacatat
     5161 tgttcatttt ttgctgttac tgtccctgac aataaaacag aggatgccac gcttccttcc
     5221 ttggcaacaa caagatgagt aggctcatct tttacagcag tctgagaagg ttgtctaaaa
     5281 agtaaaatcc ccccggcacc caatacaact acactcgcag caccgattgc tgcatacagt
     5341 tgccactttt tagctttacc attctttttc ttcataatga aactcctttt cttttttaca
     5401 atactttgct attataccaa atttccctcc agcaaacaat acagttcagg attaagcaat
     5461 cgttcggaat ttttcttttc tattgtacta gatatataat acactatctt gacttatttg
     5521 gcaagctttt ttgcaatttt ttgtgcgtag cagatttttg caatcaaatc aaaaaaagaa
     5581 taaaatataa ctatcaaaaa agacgctcta ctatttaaaa gattacaaag gagttttcaa
     5641 tgagagaata tgatatcatt gctatcggtg gaggtagcgg aggaatcgct accatgaacc
     5701 gtgctggtga acatggagcc aaagcagccg ttattgagga aaagaaatta ggtggaacct
     5761 gtgtcaacgt cggttgtgtt cctaaaaaaa tcatgtggta cggggcgcaa atcgctgaga
     5821 ctttccatca atttggagaa gactacggct ttaagactac tgatcttaac tttgactttg
     5881 caaccctacg tcgcaatcgt gaagcctaca ttgatcgcgc tcgttcttct tatgatagta
     5941 gttttaaacg caacggtgta gacttgattg aaggtcatgc tgaatttgta gattctcata
     6001 ctgtaagcgt aaatggtgaa ctgattcgtg ctaaacatat cgtgattgct acaggtgccc
     6061 atccaagtat tcctaatatt cctggtgctg agctaggtgg ctcttctgat gatgtatttg
     6121 cctgggaaga acttccagag tcagttgcca ttctaggcgc tggttatatc gccgttgaat
     6181 tagctggcgt actccacact tttggtgtca agacagatct ctttgttcgc cgcgatcgtc
     6241 ctttacgtgg ttttgattcc tacatcgttg aaggtttggt caaggaaatg gaaagaataa
     6301 acttgccact tcatactcac aaagtccctg ccaagttaga aaaaactgct aaaggcatta
     6361 ccattcattt cgaggatggt accagtcaca cagctagcca agttatctgg gctacaggtc
     6421 gccgtccaaa cgttaagagc ttgcaacttg aaaaagctgg agtgactctg aatgaacgtg
     6481 gctttatcca agtagatgaa taccaaaata ctgttgttga gggaatctat gctctaggtg
     6541 atgtaacagg tgaaaaagaa ctgactccag ttgcaatcaa ggctgggcgt accctatcag
     6601 aacgtctctt taacggaaaa acaactgcaa aaatggatta ctcaactatt ccaactgttg
     6661 tcttttcaca ccctgctatc ggaactgttg gtttgacaga agaacaagct attaaagaat
     6721 acggtcaaga ccaaatcaag gtttacaaat caagctttac ttctatgtac tctgcttgca
     6781 cttgcaaccg tcaagaaact cgtttcaaat tgatcacagc tggttcagaa gaaaaagttg
     6841 tcggacttca cggaattggc tacggcgttg atgaaatgat tcaaggattt gctgttgcta
     6901 tcaaaatgag agcaaccaag gctgactttg acgcaactgt agcgattcac ccaacttcat
     6961 ctgaagaatt tgtaaccatg cgttaatcga aacaaaagaa gctgatataa atatgtctgc
     7021 ttctttttta atgattttcg aatcaacaaa aaattatcca acttgactag tcacgaaaat
     7081 cggctgttac agtttttgtt accttgtttt taaaaaatgg ttgacatcga ctcctatatg
     7141 agtataatag ttactataca ttagaaaaga ggttaactat tttgaaaaaa gctcacgttt
     7201 atgctatccc tgctattggg gctgctctca ttgctgtttt ggcacaaatc agtcttccaa
     7261 ttggacctgt tcccttcact ctgcaaaact ttgcaatcgg cttgattgct actgtcttta
     7321 gaccgagaga ggctgtactt tctgctggac tctatcttct tctaggtgct atcggtcttc
     7381 ctgtctttgc aggaggtgga gctggttttc aggctttagt tggccctact gcaggctatc
     7441 tttggtttta tctcgtttac tctggactta cttcctctct aaccaacagc aagagtggtg
     7501 ttgttaagat ttttcttgca aacctcttgg gtgatgccct tgtctttgtc ggcgggattc
     7561 tcagcttgca tttcctagct ggaatggcat ttgaaaaagc tcttgctgtg ggggttcttc
     7621 cctttatcat tccagacctt ggcaaacttc tggctattag ttttattagc cgtcccctac
     7681 ttcaacgcct taaaaatcag gcttacttta ctaactaaaa aaggatatcg agttatcatg
     7741 actcaatatc cttttctttt attttgaaaa cttatactca atgaaaatca aagagcaaac
     7801 tagaaagcta gccgcaggct gctcaaaaca ctgttttgag gttgtggatg aaactgacga
     7861 agtcagtaac catacatacg gcaaggcaaa gctgacgcgg tttgaagaga ttttcgaaga
     7921 gtattagttg cctttaaagg catccaccat cgtttgaaat tcttcatttg agagagtaat
     7981 ccctttgccc attttagtat ggtctggact ccaagcacga atatcaaact ttgcaggggc
     8041 accattaaag ctcacacggt taatttcctt ggtccaacct ttttcgtttt cagaaagagt
     8101 caacaagtgc tcttcgattt caaatgtaaa ttctgccatt ttcttctcct tttttagttt
     8161 cattagttta ttcgtaaaat cttgtagatt ttaggaaaat tttatataat attgatataa
     8221 aagaagggag gccaatatga gacataaatt ccagcaagtt ctaaataaaa tacatgattt
     8281 tttaaatgga tatgaccaac ctgaccagac tgaaaccaac tcccttacag ccactattga
     8341 agaggctatc cagaaacaaa ccgctgttca ccttatcttg tctgagacaa gttttacagg
     8401 tgatatcatc aaatatgatc agcaaggcca gcaaattatc gtgaaaaatt ttgccaaaaa
     8461 tgtgagccgg attatccgta taagcgatat tcaacgcctg cgatttgtcc cctcaactgt
     8521 ccaaacagcc caaaaaaata gatttaagaa agagtgagat gtagttgctt catcccactc
     8581 ttttttctta gcgaatttgt tcaaaatgta aatgaactgc aatatgatct ccataaccac
     8641 ttctttccaa gtcacgttgt aaacgatagg aaatgtagtg ttctgcaatg gtaatgtaac
     8701 ctgcgcccaa taaacgatgt tcaaccatag attgaatcat actgatagtc gcacgttcca
     8761 ccttggcttc ttgtaaatcc aaaactacct tcttagtgac ttgagcaaga ttttgacgca
     8821 aatcatctgt caaaacataa acagtttggg ctgccttcaa gatggcttgg taaatcttat
     8881 ctggattaaa ttcagcaatt tcgccattac gtttgattac ttgcataggt ttctccttta
     8941 ttctttgttt tctttgattt ctgccagcat tttttcttct tctactgtca gttgataatg
     9001 ttcaagtaaa tccggtctgc gctcgtaggt tttctttaaa ctctcgtaca atcgccactg
     9061 acgaatcttt tcatggtggc cactcatcaa tacatctggc acgaccatgc ctcgataatc
     9121 atagggacgt gtgtactgag gatattctaa aagacctgaa gaaaaactat catcttggtg
     9181 gctagactcc ttgccaatca cttctggaat caggcgaact gtagcatcaa tcatggtcat
     9241 agctgccaat tctccaccag tgaggacata gtcgcctagg gaaatctcat ctgttaccaa
     9301 ggtcttaatg cgctcatcat aaccctcata gtgcccacag ataaagatta gctcttcctc
     9361 ttgagccaaa tcttcagcat aaacctgatc aaactgcttt ccagcaggat cgaggagaat
     9421 aacgcgcgga tttttctttt caatagcatc aaaggaatcg aaaataggtt gtgctctgag
     9481 caacatgccc tgaccgcctc cgtagggctc atcatctaca tgacgggcct tttcagcatt
     9541 ttctcgaaaa ttatgatact ggatatccaa gagccctttt tctcgagcct ttccaacgat
     9601 tgagtgctcc agtggagaaa acatctctgg aaagagggtt aaaatatcaa tcttcatcgt
     9661 ctaacccttc taagatttcc acatcgaccc gtttatttgg aatatcaaca ttgagaacca
     9721 ctggtgggat ataaggtaaa agcaaatcac gtttgccttt tcgtttgacc acccagacat
     9781 cattagcacc tggttgcagg atttccttga tggttccaac caagctatca ccctcataga
     9841 cttccaaacc gataatctcg tgatagtaaa attcaccatc gtctaggtca ttcaaatctt
     9901 cctcagcgac cttgagactg tatcccttgt acttttcgat agtattgata tggtacatat
     9961 ctttgaattt aataatgtca aagttcttct gtttacggtg gctagcgatg gtcactgttt
    10021 ggacaaactg atctttttca tcaaacaaag ccagctcagc tcctttttta aaccgttctt
    10081 ctgcaaaatc cgtcacagac aagactcgca tctcaccctg taatccctgc gtattaacga
    10141 ttttcccaac attaaagtag ttcatcttgt ctcctgtaat ctcctttttt ccatcttatt
    10201 ctgacaattc tcgaataata gccgcaattt tttccgattc tgaccattgt aaataatggt
    10261 gattccttcc taaaatgagt ttagtattgg aagtccaata ttctgattct ctgtactctt
    10321 tttctctata agtctgacaa aaaataaata cagggacatg agattctatc gacaaattct
    10381 caaaatcttc ctcagtaatc tctccagata tctgaaattc tggatcttga ttttccaact
    10441 ctaagccttt ttcttgcatt aattcccaga tttttttatt cgtttcagga ctaaatgttg
    10501 cttgagttaa gttcttaaaa taaagttcag gaccacactc gtcaatcagc ctcatctgct
    10561 cttccatttc tggataagga ttttctgaaa aatcagcaaa catgactttt ttagttgtcg
    10621 gttcaattgc tactaaagtc tgacgcttaa ttggtttctc gagtaatttg caagctaaaa
    10681 ttccactcca actatgtgca caaagtatat attcagaaat tcctaattct tcaagtactt
    10741 cataaaccgc atctgcaaga ttatctagat tttttccagc ttggtcatga atcggactcc
    10801 tacctgtgtt cggaaaatca attgtcaaat aaccaattgt aggaggaggt ttttcaagta
    10861 taagtgaaaa attttcataa cttggtagca aacctgctcc gtttagacaa actagcactt
    10921 tcttttgctt ttgataagta acagagaggc taccaatttc tgtagatact tcaaacctct
    10981 tcataaagaa atccactcat tctatataat gaattattaa aaatccttat cctttatttt
    11041 atcacgttcc aaagattttc tcaagttgga ggaaggggac aacatctcta ctttcccttc
    11101 aagaatcctt ccaaatcatg tttatgttgg taattaacgg ctgcggtttt gtctttctca
    11161 aagatggttc tagttaggtc aatatgcttg atagcagcaa tagcgacggt gtagtgaatg
    11221 atatcagcca gttctatggc aggttcctcg ttcgaatcct atcccttctt ttcgaccaga
    11281 gcgcctattc aaaacctcgg ctacttctcc gacttcctcc actaacttca taaagagacc
    11341 ttcatcagtc cgagactgct gttaatgttc gagtaagtag tcttggaatt gcctaaacgt
    11401 tcaatctttt atagtatatt gaaactagaa tagtacacct ttacttctaa aacattgtta
    11461 gaaatcgatt tgactgtcct gatcgatttg tcctgttatt atttcatttt actatatctt
    11521 ctattccaca caaaaaagcg agacatccgt cccgcccttc ttatttttcg tcaataacga
    11581 ttcttacttt tttgtattca gttgggacag agtagacaat cgttcttatc gcagaaatag
    11641 tgcgaccctt acgaccgatt acacgaccca catcgctttg atcaagattc aaatgatatt
    11701 ccaaaaattc tggtgtatcc tcaatcttga tagttaaggc atctggttgt gaaattaagg
    11761 gtttcacaat cgcaataatg agattttcaa tcgtatccat ctgtcaacct actttaaact
    11821 tattttgaga atttagaatc gtggaatttt ttcaatacgc cttcttttga aaggatgtta
    11881 cgtactgtgt ctgaaggttg agctccatta gccaaccatg caagaacgcg gtcttctttc
    11941 aaagttactt ggttttcagc aacaagtggg ttgtaagttc caactgtttc gatgaaacgt
    12001 ccgtcacgtg gtgaacgtga atctgctacg ttgatacggt agaaaggttt tttcttagaa
    12061 cccatacgag tcaaacggat tttaactgcc atttttaaag tctcatttct ttaatttttt
    12121 atttcggtga aatagctgag ctatttagca catgttctat tatagcagat ttctggcatg
    12181 tgtcaagaaa aaaacttgac agactataaa tttcttaaag tatttagaaa tttgttagaa
    12241 taggaaataa atgtttgaaa gatgctatcc gtgaatacta ttttagaaaa attttataaa
    12301 gaacaccaag tcaaaccctt catatcgcct gaacgagagt tggatacttg gcttctaagt
    12361 cccaagcccg ttcccaagcg aaatatggac ttattagtag atgattcact agcaggagat
    12421 atcattttac tatggagaat ccagtttggt acttttacca ctgaaacatg attttaaaaa
    12481 ctcccattcg aatatgattc aaaattcgct tagaatcaat gttttcccgc tttttcaaaa
    12541 aataaatttt catctaattt aaataacttt caattaagtg tgaattttgc ttaaaatcat
    12601 taaaaagcac cccatggtgt gaaccatgaa gtgttgtaaa tgctgtgttg tagctagttc
    12661 ttagcgttta gagaactggc tagctttacg agctttctta agatctggtt tccaaaatac
    12721 tttgagtaca cttatggtat tgatgcccct aaacatctaa aaactctggt agaaaaaggg
    12781 tatgctcttg tcgaaacttc ttttgactca cttgaccatc taaataatac catgaaaaag
    12841 aatatcctga agaagaaggg agttactgga ctttctaaaa tgaaatcttg cgacctggat
    12901 caagcccttc atgagcattt ttcagaagaa gaattagctg gtcactttca tgtccttcta
    12961 tggacttttt tacaatggca ttgctatcac acccaatacc tatctaagcg cctggttcgt
    13021 aaactttatt gcagctcttc ctctaaattt cctaattgtt gaaccaattg cccgttttat
    13081 actaagttct tttcagaaac catttactgg ggaagaagtt gaagaagttg aagattttca
    13141 agatgatgat gaaatcccaa ctattatcta agccagttct gtaaactact aatatttgaa
    13201 atccacttct ttttagggta caatggtata aatgaatttt tgagaggatc agaatgaaaa
    13261 aactagcaac ccttctttta ctgtctactg tagccctagc tgggtgtagc agcgtccaac
    13321 gcagtctgcg tggtgatgat tatgttgatt ccagtcttgc tgctgaagaa agttccaaag
    13381 tagctgccca atctgccaag gagttaaacg atgctttaac aaacgaaaac gccaatttcc
    13441 cacaactatc taaggaagtt gctgaagatg aagccgaagt gattctccac acaagccaag
    13501 gtgatattcg cattaaactc ttccctaaac tcgctcctct agcggttgaa aatttcctca
    13561 ctcacgccaa agaaggctac tataacggta ttaccttcca ccgtgtcatc gatggcttta
    13621 tggtccaaac tggagatcca aaaggggacg gtacaggtgg tcagtccatc tggcatgaca
    13681 aggataagac taaagacaaa ggaactggtt tcaagaacga gattactcct tatttgtata
    13741 acatccgtgg tgctcttgct atggctaata ctggtcaacc aaacaccaat ggcagccagt
    13801 tcttcatcaa ccaaagctct acagatacct cttctaaact ccctacaagc aagtatccac
    13861 agaaaattat cgaagcctac aaagaaggtg gaaaccctag tctagatggc aaacacccag
    13921 tctttggtca agtgattgac ggtatggatg ttgtggataa gattgctaag gccgaaaaag
    13981 atgaaaaaga caagccaact actgctatca caatcgacag catcgaagtg gtgaaagact
    14041 acgattttaa atcttaaaaa ccaaaaaaat acagtatcca cattcggtac tgtatttctt
    14101 ttactctcat tcttaagtta aattattaaa atcccatatt tggtctatcc agccttcata
    14161 aaagtctggc tcgtggcaga ccataaggat agatccccta tattctttga gagcgcgttt
    14221 gagctcatcc tttgcatcca catccaaatg gttggtcggc tcgtccagca ctaaaacgtt
    14281 gttttcacga ttcatcaaga gacagaaacg aaccttggct tgctctcccc ctgataatac
    14341 ttgaatctgg ctttcaatat gtttggttgt caaaccacaa cgggcaaggg ctgcacggac
    14401 ttctgcttga ttaagggcag gaaaggcatt ccagacagct tcaagaggag tttggcgatt
    14461 accgccttct acttcctgct caaaataacc aagttctaaa taatctccac gctccacttc
    14521 cccagcgatt ggcgagataa tgcccaagag actcttcaag agagttgttt ttccaatacc
    14581 attagcacca ataatcgcaa ccttttgatt gcgttcgaag gtaagattta aaggcttagt
    14641 aagaggaagg tcgtaaccaa tttgcaagtt cttggcttgg aagataaagc gccctggtgt
    14701 acgagctggt ttgaaatcaa aggatggttt tggtttctca ctttggagtt cgataatatc
    14761 catcttatcc aatttctttt gacgagacat agccatatta cgagttgcaa cacgggcttt
    14821 attacgagcc acaaagtcct tgaggtctgc aatctctttc tgctggcgtt cgtaggctgc
    14881 ctctagctga gatttcttca tagcataaac ttcttggaac tggtagtagt caccagagta
    14941 acgcgtcagc tgttgatttt ccacatgata gacaatattg ataacgtcat tgaggaatgg
    15001 aatatcgtgc gaaatgagaa caaaggcatt ctcatagttt tggagatagc gcttgagcca
    15061 atcaatatgc tcagcatcca agtagttggt cggctcgtcc aacagcaaga tatcaggctt
    15121 ttcaaggaga agttttgcca aaagcacctt ggttctttgc ccacctgaca aagaagttac
    15181 atccgtatcc atgccaaagt ccataacacc aagagcacgc gctacttcgt caatcttagc
    15241 atccaaggta tagaaatcac gactctccag acggtcttga agttctccta cttcttccat
    15301 gagagcatca acatccgcac cgtcttcagc catttccata tagaggtcat tgatacgagc
    15361 ttcagctttg aaaagctcat caaaagccgt acggagaaca tcacgcaccg actgtctttc
    15421 agcaaggaca gagtgctgat ccaagtaacc agccgtcaca tatttggacc actcaacctt
    15481 tccttcatct ggcagcattt taccagtcac gatactcata aaggttgatt ttccttcacc
    15541 attggcaccg accaggccga tatgttcccc cttgaggaga cggaaggaca catcttcaaa
    15601 aattgcacgg tcaccaaaac cgtgactcag atttttaact tctaaaatac tcattttaat
    15661 tccttatctt gtttttatgt aatcgtttat aaaggagcca agccagatag ccacccaaag
    15721 tgttggtcca caaatcatca atctcaaaga cgcgattgaa atcaaagaaa aagtccaaga
    15781 ttaattgcgt acactcgatt ccaagactca caagaaaact aaaaagaagg accttttttg
    15841 ttttccgcaa atttggaaat agataaagga gttggaaaat cagaggaaaa aacaagaaga
    15901 cattgaggat attttgtaaa aaaatccaac ataattgtcc aatgtcactc acttcgccca
    15961 gtttccagag agaattgaaa ggagtcaaaa gaaaaaccag gcgtccaaga tgctgaatat
    16021 ctggagttcc cactcccacg gtagattgtt cttgaggagt aaagcaaaaa tagacaatgc
    16081 aaatgctata gaaaatgact ccccagacca aaatatgatt ataagtcttc ttcatcatta
    16141 aggatttacc gctgcgactg ccttctggcg gtcacgtttc attgtgttaa agagcaattg
    16201 tccacaagct gcgtcaatat ctgtaccatg ctcttgacga accacacagt tgcccccttt
    16261 tttcttaagc gtatcataga aagccaacac gcgctctttg ggactacggc tatattggtc
    16321 atgctcacta actgggttat aaggaatcaa gtttacataa gacgatttct tgatgttctt
    16381 gagcaattca gccaattcca aggcttgttc cacaccgtcg ttgacttcat taagcatgat
    16441 atattcaaag gttacacgac ggtttgttgt ctcaatgtag tattcaatag cagcaaagag
    16501 tttttcaatc ggaaaggcac ggttaatctt catgatactt gaacgaagtt cattgttggg
    16561 tgcgtgaaga gacacggcaa gattgacctg aaccccttca tcagcaaagt cacgaatttt
    16621 atgagccaaa cctgagattg aaaccgtgat gtgacgagca ccgatagcca ttcctttatc
    16681 atcattgata gtacgaaaga aattcaagac attgttgtaa ttatcaaagg gctcaccgat
    16741 tcccatgaca acgatatggc tgatgcgttc atcctgacca cgctcatcaa agtatttctg
    16801 aaccagcatg atttgcgcta cgatttcacc gttattgagg tcacgctgct tcttaatcaa
    16861 accagaggca cagaaggtac aaccgatatt acagccgacc tgagtggtca cacagacaga
    16921 taaaccatag tgttgacaca tgagtacagt ctcaattaac ataccgtcag gcaattcaaa
    16981 gagatatttg actgtaccat cagcagactc ttgcacaata ccttgtttca agggattgac
    17041 cacaaactgg tcattgagct tagcaatcaa atccttggaa aggttggtca tttcttcaaa
    17101 tgactgcaca cgtttacggt agagccattc ccagatttga tctgcacgga atttcttttc
    17161 tccctgctcc aatacccatt cctgaatggt ttgatgtgtc aaactatgaa ttgagggttt
    17221 catttcttct ccttattctc tactcacttc tgacgaatga caaaatgacg ttgttccttg
    17281 tcctctttct gagaatgctg gtctttctga cgacgtctat ttttcttatc tgcattcgac
    17341 tttcgtttag tttgagtcgg tttctttcct tttctagaag gtgtttcttc ttccgtctta
    17401 cgcattttct tgtcaaatga tgctcgctta ggggcttcat tttctaagac aaaataggca
    17461 caaccataac tacaatactc taaaaggtag tcttgtaaac gactgatttt ttcaagtttt
    17521 tcttctgttc ggtcatcctt gtaaaaacct cgtaggcgaa gctgttcgtt gctccagtcc
    17581 cccacgatat aatcaaactt ggttaagact tctgaaaaac gctgattaaa agtcgtcaca
    17641 tcaaaggcat ccttgatatt ttcaaccaag gaaaaagcta tcccttccgt ttcgacctta
    17701 tccccgtgta aatggaactc cggaccagga aacttgttat agttgtataa ttcaggtgca
    17761 atttcttttc gcatagatat cctttttcca cgattactta atactttatt ctaccataat
    17821 ttctagcagt tagcacgttt ctcataaaaa tgaaaaaagt ctgacgattt tgtcagacca
    17881 gaatcttata acctaaaaag agaagaacaa ttcttccctc caactatcat tatttagcag
    17941 ctgcgtacaa ttcatctact ttattccagt tgattactga aaagaaagct ttgatgtagt
    18001 caggacgcac gttgcggtat ttcacgtagt aagcatgttc ccaaacgtcc aagcccaaga
    18061 ttggtttttt accttctgag attggtgtgt cttggtttgc tgttgaagtc acttcaagtt
    18121 tcccttcttt gttgacaacc aaccatgccc aacctgaacc aaaacgagtt gttgctgctg
    18181 cagtgaaggc tgcttggaat tcttcaaatg aaccaaatgt tgcatcgatt gctgctgcca
    18241 gttctgctga aggagctgtt ttctcgggag tcatcaattc ccagaaaaga gcgtggttca
    18301 agtgtccgcc accattgttg ataagtgctt gacggatatc agctgggata gattctacat
    18361 cagcaagcaa ggcttcaagg tcttcaccga tttcagggtg tttttctaaa gctgcattgg
    18421 cattgttgac ataagtttga tggtgtttgt catggtgcaa atgcattgtt tccgcatcga
    18481 tgtatggttc caaagcgtcg tatgcatatg gaagttctgg taagataata gccatctgta
    18541 atacctcttt ttctttctat atgaaaatga taacgcaaac attcactttt ttcaagtttt
    18601 ttgcttatag ataaggaaat cactgacata ctttctaata aaataagcaa atcagcaaga
    18661 aacccacgac aagataatcc tgacgcgggt tgtagtttca acaaatgtca attgacctga
    18721 ctagcaatct gtaagagtgc cttttcaaaa aggaaacctt tttcataaag acctgtctta
    18781 atctgatagt ctgtctcaat caaataggaa atagcttgct tcagaaagct caaagaaagt
    18841 cctctcgaat ctcttaatgc aaacttgatt tgataaggat ttgggttacg tcccagataa
    18901 ctacctaaac tacttgcaat ctgcgattct gtttggccag actccgccaa aatcttcacc
    18961 tgagtaaaag tccgaaattg tcccagcatg actgcaatca gtttgatttc atcttcccct
    19021 tgcaaggtca agtctctcac caaatcgcgc gcctgatcca tctttttagt cagaataaac
    19081 tgagttaaat caaaaatatt gtcctgcaag gtcttgggaa ttgcgttaac aatatcctct
    19141 tcctcaataa cagaattcgc cttataggac tgtaaaaaga gaagattttt ctggatttcg
    19201 ctaaattgaa accccgactt gatgaggaga ttttcaaaag aatgattggt aaactgcaga
    19261 ccttgtttct gactccactt ttggaaatac tggcgcaatt cttgttcttt tacttctact
    19321 gcatcgaaga ccttggcatc acgcttaagt aatttgacta accgtctttt gctatccagc
    19381 tttccttctg caaagattat caacttggtt gttggagaag gattgtcaag gtattcctca
    19441 aatgacttaa gctcatcatc tgtcaaaaag cgtttcttag cagtcgtgat atccataaaa
    19501 taatctaata tcacgatttt ctcatccgca aagaaaggaa ggctgaccaa ctccagttcc
    19561 acatccttgt aaactacttc tttcatatca aagtaggcaa agttgaggtc agcagaatca
    19621 tacccaatct gtttcaacat ttgactcttc atcacttcaa actgaccctg atctgtccct
    19681 gtaaataggc tcaggctcgg taaatttgat aaagtcaact tctgactttc ttcaatggct
    19741 agcatcgtct ctcctttctt cagatttttt gatttaattt agtcaatata gcgcaatttc
    19801 ccacggaaat cttctaagct ctcgtagcct ttttccacca tgattgcttt cagttcattg
    19861 gtaatgcggt caaaagcact gacgccttct ttgtgaaggg tcgttcccac ctgcaccata
    19921 cttgctccac agaggatgtg ttcaaaggca tctcgaccag tcagaacgcc acctgttccg
    19981 ataatttgga tttgaggatt taaacgttga taaaaggcgt gaacattggc tagagcagtc
    20041 ggtttgatgt attctccacc aattccacca aaaccattct taggccgaat aacgacagat
    20101 tcgtcttcta tatagaggcc gtttccgata gagttaacgc agttgacaaa cttgagcgga
    20161 tatttgttga aaatagctgc cgcttgatca aagtgaacaa tatcaaaata aggtggcaat
    20221 ttaattccaa gaggtttggt gaagtaagca aacacttctg ccaaaatccg gtctgttgtc
    20281 tcaaaatcat aggcaatctg aggtttacct ggaacatttg gacaggaaag atttagctca
    20341 gtcagaccac gaaaatcact ctcttggact tttttcaaaa tagtatgggt ttcctctgga
    20401 gacatgccga ccagagataa gaagaaagtt cggttcgact ctttttcctg caaatctaaa
    20461 agataatcca aataatagtc taagccatta tttggcaagc ccatagagtt gatggaacca
    20521 agtggaacat cttggtagcg tggctcagga ttcccctgac ggaagtccaa ggtcgctgtc
    20581 ttagtaacaa aggttcctgc cgctgagttt ttgacctctt ctaactcctc tatcgtcata
    20641 caagccacac ctgctgcatt catcaagcaa ttgtcaaact caaaaccagc aatttgtgtt
    20701 ttcgttgata ccatgattct aatcctccag atttctttta ttgctaatat tataccataa
    20761 ttttaattct aggtttacta gattctgtgg ttctatctat ttattcggaa aagaaagtct
    20821 agtacagaag tcatgctatg gaaacagcta gtgtattgga agtttgaaca aggcttgggt
    20881 aacagacttt tgaatgaagt ccaagcaaca aggagaaatc tcaaccatga gtccattcca
    20941 tgactctgtt gatctagaaa accttgcaat agtcaagtga taacgaataa aactttggct
    21001 tgaaatatcc ccatgattat ctattacgag gagaaatctc aacctttatc atttttaaag
    21061 aaatatagta aaatgaaaca agaacagg
//
LOCUS       DAVBDG010000032        20922 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBDG010000032 DAVBDG010000000
VERSION     DAVBDG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.032"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..381
                     /locus_tag="VQ526_001226"
     CDS             <1..381
                     /locus_tag="VQ526_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET2554951.1"
                     /translation="LTNKEILNKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIE
                     DNLKQAHPIFQTVFKTFLKDKEKIINALQLHYSNAKLEATNNLIKLIKRNAFGFRNFE
                     NFKKRIFIALNIKKERTKFVLSQA"
     gene            complement(774..2501)
                     /gene="ezrA"
                     /locus_tag="VQ526_001227"
     CDS             complement(774..2501)
                     /gene="ezrA"
                     /locus_tag="VQ526_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2554952.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2583..4529)
                     /gene="gyrB"
                     /locus_tag="VQ526_001228"
     CDS             complement(2583..4529)
                     /gene="gyrB"
                     /locus_tag="VQ526_001228"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2554953.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4544..5107)
                     /locus_tag="VQ526_001229"
     CDS             complement(4544..5107)
                     /locus_tag="VQ526_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2554954.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENSLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(5192..5746)
                     /locus_tag="VQ526_001230"
     CDS             complement(5192..5746)
                     /locus_tag="VQ526_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2554955.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5757..6980)
                     /locus_tag="VQ526_001231"
     CDS             complement(5757..6980)
                     /locus_tag="VQ526_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2554956.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(7017..9467)
                     /locus_tag="VQ526_001232"
     CDS             complement(7017..9467)
                     /locus_tag="VQ526_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2554957.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKMAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQNLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEVKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9667..10596)
                     /locus_tag="VQ526_001233"
     CDS             complement(9667..10596)
                     /locus_tag="VQ526_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2554958.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10736..11023
                     /locus_tag="VQ526_001234"
     CDS             10736..11023
                     /locus_tag="VQ526_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2554959.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(11057..12391)
                     /gene="ciaH"
                     /locus_tag="VQ526_001235"
     CDS             complement(11057..12391)
                     /gene="ciaH"
                     /locus_tag="VQ526_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2554960.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12381..13055)
                     /gene="ciaR"
                     /locus_tag="VQ526_001236"
     CDS             complement(12381..13055)
                     /gene="ciaR"
                     /locus_tag="VQ526_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2554961.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(13164..15710)
                     /locus_tag="VQ526_001237"
     CDS             complement(13164..15710)
                     /locus_tag="VQ526_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2554962.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEISGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15833..16273)
                     /locus_tag="VQ526_001238"
     CDS             complement(15833..16273)
                     /locus_tag="VQ526_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2554963.1"
                     /translation="MTPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(16248..17117)
                     /locus_tag="VQ526_001239"
     CDS             complement(16248..17117)
                     /locus_tag="VQ526_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2554964.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(17095..17595)
                     /locus_tag="VQ526_001240"
     CDS             complement(17095..17595)
                     /locus_tag="VQ526_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2554965.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVFKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17606..18304)
                     /locus_tag="VQ526_001241"
     CDS             complement(17606..18304)
                     /locus_tag="VQ526_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2554966.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18297..18533)
                     /locus_tag="VQ526_001242"
     CDS             complement(18297..18533)
                     /locus_tag="VQ526_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2554967.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(18633..19559)
                     /locus_tag="VQ526_001243"
     CDS             complement(18633..19559)
                     /locus_tag="VQ526_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2554968.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19634..20449)
                     /locus_tag="VQ526_001244"
     CDS             complement(19634..20449)
                     /locus_tag="VQ526_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554969.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVICIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20453..20773)
                     /locus_tag="VQ526_001245"
     CDS             complement(20453..20773)
                     /locus_tag="VQ526_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2554970.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6312 a         4582 c         3695 g         6333 t
ORIGIN      
        1 ttaaccaata aagagatttt aaacaagctt ttgagctatt cggaagactt gaaacaccac
       61 tatcagctct atcagctctt gctgtttcac ttccagaata aggaaccaga gaaatttttc
      121 ggacttattg aggacaatct taagcaggct catcctattt ttcagactgt ctttaaaacc
      181 ttcctcaaag ataaagaaaa gattatcaac gcccttcaac tacactattc taatgccaaa
      241 ctggaagcga ccaataatct catcaaactt atcaagcgca atgcctttgg ttttcgaaac
      301 tttgaaaact tcaaaaaacg gatttttatc gctttgaaca tcaaaaaaga aaggacgaaa
      361 tttgtccttt ctcaagctta gctgacttca acccactaca gttgacaaag agcccagatt
      421 aggaactatc cgtgagttct ctagtctgga gatttttcaa tagacttcgt tattgaacgg
      481 ttaccaagtt actcaaagta cagatttgaa gctgcagata agactgacga aatcacttta
      541 atactaaatg aaaatcaaaa agcaaactaa gagcctcagg ctgtacttga gtacggtaag
      601 gcgacgctga cgtgatttga agagattttc gaagagtata aaacactgtt ttgaggtggt
      661 ggataaatcg gctgggcaaa aactcaatct caggagaaaa tgaagtaaat ctccccacaa
      721 ctatagaaaa agatttgatt ctgctcctca cacaataaaa tctttttctt ttattaaaaa
      781 cgaatcgttt cacgtgtttt ctcatatgag gtaacaaagc gattggttac accaggctct
      841 gccacttcca atgcttgaga aatcttgtca aatgaagcgt gataatcaaa ttctttttca
      901 aaaatatcta aagcttcgtt aaatgcttct tgaatgcgtt catcaaatga gcgatagcgg
      961 ttagaatatt gcaagagttg ctctgtcaaa gttgcatatt gtacaatatt ataagtttcc
     1021 gtttctaaag cttccatatc attcgttgca atttcaagaa ctcgggtaac agattcaatg
     1081 ttaatcattt tttgttctaa ctcaaccatt aaatcctcgg tattattgct tgccgtaaag
     1141 aataacttca agaaagtttg tggaatacct ggcagattgc gtttttccat gtatcgcttg
     1201 atagtatgga gacgattgac ataaacattg gccttttgac gtgcattaat atcatctttc
     1261 tcaatttgtg tcaggcgctc actaactgaa atttgctcat cttcaatatc ttttagttga
     1321 gtttgtaaat cctcaagatt ttcttcaaga actgaataag cttgggttgg ttcttcttga
     1381 tttgaagtta cctcaacaat agctgcctca aaactctcta attctgtctg aatacgacga
     1441 acatggcttg cagctgtctc aggaagtaaa taggtcttgt tcaaacgtgc aatatcttct
     1501 cccaataaag tattattctc tttcatatgt tgaaggtaag ttggaagagt tgcaagtaga
     1561 ttttccacta ctttctgagc agcaatttct cgagtaaaaa tatcatacaa ggcattgatt
     1621 tcctcttgtg cctgtccatt ctcatattcg gcattatcca attccaactg acgaatattc
     1681 tcttggtttt tcttgaatgc ttcataaagc aagtggaaac gcgcttcaat atccgtttca
     1741 acaaaatgat aattagcatc aattagttta cgataaccgg cttccaaatc ctgtaattga
     1801 tctggcaatt ctgtagaaag cgtagtaacc aaggctggaa cacgatccac aatatgactt
     1861 aaggccaaaa tgtgattttc tgtattatcc aaaatcactg cggcttccac agggtcaccc
     1921 gatgaattca aggttacaaa ttgtgaaaat tcagattgga tattttctaa ttgtttttca
     1981 atttcatcca aggcttgacc atactgttct gaattttcag caactctatg ctgaagttcc
     2041 tcaaataaat ccaaagcatg aagaacacga ccactatttt tagattcttg cttctctaag
     2101 tctgccaaag cattgcgaat tgccgcaata tcttcttcaa tcaaagtaat ttgactctca
     2161 atttggtcaa tttgatgact ggccttgaga aaacgaaatg aatggttata gccctctgct
     2221 tcaaagagat tattttcaat atcggcaaaa gagttgagag ataaatcgac ccatttttga
     2281 ttccattcac ggaaagccac ttgactttgt ccaatcaagt gcatattttt tacagcttct
     2341 acttcatcat ttactggaag attgtatagt tcttcttttc tttcttctag tgcctctaat
     2401 ctcccctcgt ttcgcttacg tagaaagatt gccactacat aagccagaac taaaatgact
     2461 gcaattgcaa ccattaaata aattagttgt ccattagaca tatcaaactc cttttttact
     2521 tgaaacaatc gtaacgatta tatcatattt tttagaccaa gggaactact tctccctatt
     2581 ttttagacat caagtgtact atagacagca ttttcttcga taaactcacg acgaggctct
     2641 actcgatccc ccatcaacat atcaaagatt ttatctgctt ctgcagcatc atctacagaa
     2701 actctagcca tcaagcgatg ttcgggatcc atggttgttt cccacagctg atgatcgtcc
     2761 atttcaccta gccccttata acgctgaata gtcggtttgg tacgaccttc actataacgg
     2821 gctaaagctt cttggagttt gatttcttga tctgcacccg gctggatata ttctttaatc
     2881 tcgcttccaa ccttgacacc atagattggt ggttgggcaa tataaacata accagcttct
     2941 aggattggtt tcatataacg ataaatcaag gttaaaagaa gggtacgaat atgggctcca
     3001 tcgacatcgg catcggtcat caaaacgagt ttttggtaac gggctttcga aacatcaaat
     3061 tctgcgccaa atcctgttcc catggctgtg aaaagactac gaatttcttc gttagctaga
     3121 atcttatcca tacttgcttt ttcaacgttc aaaatcttac cgcgaattgg aaggatagcc
     3181 tgaaactcac ggttacgacc agatttggct gatccaccag ctgagtctcc ttcgacgatg
     3241 aagagttctg tttcagcagg gttattagaa gaacagtctg ctagtttccc tggaaggttg
     3301 gaaatttcca aaccagattt tttacgtgtg acttcacgcg cacgcttggc agccacacga
     3361 gccttggcag ccaaaattcc tttttctacg atacgtttgg caatctgtgg attttccatg
     3421 aggaaatcgg agaaggcttc actgaagagg cgattggtaa tcttgaccac ttcgctattt
     3481 cccaatttgg tcttggtttg tccttcaaac tgtggatttg ggtgtttaac tgagataact
     3541 gcagttaatc cttcgcgaac atcttcccct gttaaattat cttcattgtc tttcagtaac
     3601 ttattcttac gagcataatc attgataaca cgagtcagtg ccgtacggaa accttgttca
     3661 tgcgttccac cttcatgggt atgaatatta ttggcgaaac tcatgacatt ttcatggtaa
     3721 cctgttgtgt actgcatggc tacctcaact gtgatatcat ccatctcacc gtctgtatag
     3781 attggtgtat caaagattac atccttgttc tcgttgatat attcaacgta actagcaatc
     3841 ccaccttcat aatgataatg cttggtttgt tccaaacctt ggcgcttatc tgtaattgaa
     3901 atttgaagac cgcgatttag aaaggccaac tcttgaatcc gtttatttaa tttatcaaaa
     3961 tcaaagattg ttgtttcagt gaagattttt gggtccggtg tgaagtgaac agttgttcct
     4021 gttttatccg tatctccaac tatttcaaga tctgcgacaa catgaccacg acggtattct
     4081 tggtaatgaa tcttaccatt tttgtgaaca tgaacgtcta attgagtgga aagggcatta
     4141 actactgacg accccacccc gtgaagacca cctgaaacct tgtatccacc accgccgaac
     4201 tttcctccag cgtgaaggac tgtaaagacg gtctcaacag caggacggcc tgttttttcc
     4261 tgaatatcga ctgggatacc acgcccatca tccacaacag taatcgaatc atctggctca
     4321 ataaaaactt gaatatggct ggcaaatcct gccaaggcct cgtcaattga gttatcaaca
     4381 atttcccaga ctagatggtg aagaccttct tttgaggttg atccaatgta catccctgga
     4441 cgcatacgaa cagcctctaa gccctctaaa acttgaattt gactggcatc ataatcctgt
     4501 gcctgcagat ttttgatttc ttctgtcatc ttattccttt ttcttatatg tctaatactt
     4561 gtcttaaatt cacgatactg gtaaacaagt gggtatcaag tcctgcagct tgacctgctt
     4621 cgatatcaat cggccgatca ccaatgacaa gaccagagct aatctgatac ttttctctta
     4681 aataaagcat ggattcggga tttggctttc tcttaaagcc tgagctagaa gtcaccactt
     4741 ctgtaaaata agctgctata gaggtttttt ctaaaatttc caaaacctga tcatttcgat
     4801 gagagaccaa aaaatgacgg ccaccttgat ttgaaatgac ttccaatagg tcagaaactc
     4861 cttcaaataa aatcgggtgt tcaagctctc tggcttcatt ttccttgtac ttttctaaag
     4921 aattctctaa attgggagcg aatgtctcaa tcgcaaaagg agtagaaacc tttaaagctt
     4981 gatagacact gtcatggtct tgtgtgatac catacagtgc caatgtttca acaaatgcag
     5041 ctgttgaagt ttcataatta tccagtaaag ttccacctaa atcccagatg taatcgtgat
     5101 atttcatatc cttcattata gcattttttt atgaaaaaag cgatgatttc cctgagtttt
     5161 ccacataaaa aacgagagaa taactcccgt tttactgatt tttaccaaag acatcttgat
     5221 agagcattcc tgttcgtaaa ctctctgcgt cccctcctag ttgctccacc aactcgtagg
     5281 caaaggcaag ggctgttgaa ggaccccgac tggttgtcaa ctgaccatct accactactg
     5341 tttccttgac gtagtgacca tcaaggattt gctcttgaac gccgtcataa caagtgtatc
     5401 gcttattttt caatatctct gcttgattga gggcaattgg tgccgcacaa atggctgcta
     5461 gtttcttccc ttcttgctcg aagctttgca attcttgaat caaggtctga ttatcacgta
     5521 aatgtgcaga accaggcata cctccaggaa gaacaatcat atcatagtct gataaatctc
     5581 catcaaagac atgatctgct cttacttgga ttgcatgcga acccgttact tgctcttcaa
     5641 aaccaaccat atcacatgtg atattggctc gacgcaagac atctacaact gtcaaggctt
     5701 caatttcttc aaagccctga gctaacataa ctgctacttt taccatgatt ttctccttat
     5761 ttaatttgtt ttaatacaac ctttttccgt ttgaatggga cttttccgct cttttcttca
     5821 gctagattag tctggtaact catcgataaa agcaaaccaa caccaatcag attactgata
     5881 atagctgatc ccccttgcga aataaaaggc aagggaatcc ccgtcaaagg aagtagtcca
     5941 gtcacagcac cgatattctc aaagatgtgg aagagcaaca tcataatcaa acctgtggaa
     6001 atataagtgt agaactggtt atttgattta agagtaatct tcaacatacg gtaaatcaac
     6061 atgagataga gggcaataac caggacagag ccaataaagc caaaatcttc tgcaataacc
     6121 gtaaaaatca tatctgactc tcgaactggg ataagcagat tcgaagcatt aaatccctga
     6181 ccaaataagc caccactccc aatggcaatc tgtccttgag cctgctggta agtcgttgtt
     6241 tgggcaaact caaagggatt gagccaagcc aaaatccgat taatttggta ggtcggcatt
     6301 ccaatctggt gaagaaaagc tcgtccgtcc ttgctaataa agatagctaa gaaaccagca
     6361 actcctgtta cagcagtcac aaatactggg ataataattt tccaagaaac ccctgataat
     6421 aaaacgattc ctgagaaaat ggctacaaaa accaaagccg tccccaagtc actttgaagt
     6481 gctaaaagaa ctaggactgg aatggtaaag agaatcatcc agaaaattaa caaaaagtcc
     6541 agcggaaccg tgcgtctcca ttccttatgt ttctttgtaa attggacaat gacacgagcc
     6601 aacatgagga tataggatat cttcataaat tctgacggtt ggaatagggt aattccattt
     6661 attgataccc agtttttggc acccgttgat gcaactaagc ttggattata aaatacaatc
     6721 ggcaagatca taagtcccaa gcctaaaata tatagaaagg gggtcacctt ccaaagaaat
     6781 tctgtattaa agagcatgac cacaaaacca atcacaagcc ccaaggcaat ccaggcgacc
     6841 tgctgcccta aaatgggcag aatattattg ggataatcat gactaacggc tatatagata
     6901 gccaccacac cgatgaccag tagaaaaaat actggcaaga gcaaactgta atcgactctt
     6961 gagtcgagag aacgtttcat atatactaac cttatacttt catacaatac tatttatcaa
     7021 agttcattaa aaaatctatc aatagcctcg tcaacttcgg atcgagagat ggttttaaca
     7081 gtcgcttctt ctgctagaga tgctactatt tgtttgccgt atcgttttcc gacgattctc
     7141 ctatccaaaa taagagttaa ggaacgttgg tattcacgtc tcatacttct tcccaaagcc
     7201 tgttttaaac gaataatggc cattggcaat tgataatcat aaaaggcatt tttcccttct
     7261 tgattcagtt cttgattaat ctttttcgtc aagggttctt gaggattttg gaaaggaagc
     7321 ctcggtacaa cttgaatcac aaaaggatgg cttgaaaaat caactccctc ccagaaactt
     7381 gctgcaccaa gcaagatttg ttgttcacct ttttcaaagc gtttctttag ctgatgaaca
     7441 tccccatttt tatactgggc caagtggcta actgtaagta aatccgatac tgctagaagc
     7501 atgtctttag cggtaaagag aaccaaaatc ggttgctgga aagcttgaat ttccactagt
     7561 aaagcagcta cctctctggc atagacttct aaggaggttt ctgttaccag agggaaatct
     7621 ttgaccataa ccacttcttg ttcctgtttt ccccgagatt caatcttgac aaatttgact
     7681 tcaggatagc ctaaaaggtc tgccaaagaa accctctgac taatctcaag agtagctgat
     7741 actcccaaga cttggcaact ctcaggaagt aaagaggaca gaagagtacg gcctgatttt
     7801 gtagaagaaa tctgaatttt cttttgactc gtttcagtta cttcaagcca gtaatcacct
     7861 tcagctgtaa aatagcgaac cagctcatca aagccttcta cttctaattc tgaaaaatac
     7921 tgatggagat tgtccagaga atctaagata tttttcctag atttaccaga ctgaaattgt
     7981 tctatcaagt agagacactc aaagcgaata ctttctagta tccgttgttg aaccctgttt
     8041 tcttctccta ctaaagcctt atcaattaaa tcgataatag attgtatatc gtaggtctct
     8101 tgaagcagat tttctagagc taacaaaatc ttttggactt catcaataat cagtaaacgg
     8161 tcactgacaa attcaggatt atcttcaagt ctggttacga gataggcatg attagtcact
     8221 aaaagcttgc aagtctctgc cctttcttga ctacgtttcc aaaaatcttc cgtcacaaat
     8281 aagttctggg atgataaatt cccatcatga cgaaggtctg ctagaaaatg ttggtaacgg
     8341 tagagttgcc cgatttcatc caaatctcct gtctctgtct cagtaagcca gaccaagact
     8401 tgcattttaa agcgtctaaa taagcgattt tcatcatttt cctgcaagga atgataaaag
     8461 gcatccaact tcagataatt ttgtggtccc tttaagctat gaatatctgt atggaacact
     8521 tccttgaggc gtttaccttc ttcttccatg atttgatttt gaagaatctt tgtcggaaca
     8581 ctaagaacaa tttgtcgctc tttggattga gataaagcgg gtaagagata gccataggtt
     8641 ttcccaatcc ctgtcggcgc ttgaatcaga gagacaggtt catctttcaa tagcaagcca
     8701 acctctttag caaaactttc ttgttcctcc ctcacttcaa ggttcaacag agaaatattt
     8761 ttagaaaagt cttgagatag ttttcgtggc tccagagaag ctgccatttt cttaaaatac
     8821 agaccttgaa cttggaccaa gtctggagaa ctcaggatag attggttgcg ataagtttcc
     8881 tcaataacca ggtaggactc atataggaga gcgtcagcca tttccagcaa gcgttccaag
     8941 agacctttag gaagctgggt catcttttcc cgtaaaaaaa gaagtaattc tgctgtagct
     9001 tgggcatctg aaagggctgt gtgtgcgtgt ttaagaggaa ttcctaattc tcgacacaaa
     9061 atcggcaagc tatatttttc cagttcaggg aaaaagacct gggccaattc gaccgtatca
     9121 acacgagggt ttcttagctc atagccttca aaaaataaat tttccgccaa gagattagca
     9181 tcaaactgaa cattatgggc tacaaaaatc ccatcctcca ccaagtcaaa tatttttctg
     9241 gcaacttgcg aaaaatcagg tgcttgcgcc agacgttggt ctgtcaatcc tgtcagttct
     9301 ttgatatgag catccaaggg ttcatgtgga ttgacatccg tcgtatagtg atcgacgatt
     9361 tctccatcct caatcacgac aattcccact tggataattt tagccttact acctgtgcta
     9421 gttgcctcta aatccaccac aacatagcga ttaccaatcg ttttcattat aaaaaattat
     9481 accaaaaaaa gggccatttg gcacctacaa acataggata attttcaaac tcaagaacac
     9541 tacttttctt aaaaatccca tcgaatagag cattctagcc tgctgatttt tgtaaaatag
     9601 tgaagaaata cgaaaaattg agagaagaaa tgtttgcatc ttccttctct caacgaatca
     9661 tgtattttat ttaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt
     9721 tttagggtga agtttacgaa cttcttcatc cagcaagaag gcttgatcat ggctaaagtt
     9781 gccctcttga atcaagttac gcgcttggat aaagagtttt gcaatctcaa caaagttctg
     9841 accaggagtg tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt
     9901 taatttgatc atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc
     9961 aaaaatggaa tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg
    10021 cgtcttttca tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga
    10081 atgatggatg acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg
    10141 cattttgaaa agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc
    10201 cttttgtccc cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat
    10261 ggtcttaaat gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt
    10321 aatatagtct gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat
    10381 atcttggtgc atgagagcat tgaccgcaac gatcaattcc ttaccagcat catgaaccaa
    10441 cttagcgatt tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa ggccaaaatc
    10501 tttctcaccg acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat
    10561 actttcagca gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc
    10621 caaaatcatc catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta
    10681 ataattctct gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaattatgcc
    10741 cgtaagaaaa ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc
    10801 tcattaccaa gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt
    10861 ttttattaat atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc
    10921 attaaaatta tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt
    10981 aaaagttcaa cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc
    11041 agttggagcg atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt
    11101 caaagattgt tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta
    11161 caatttgctt ggctagggat aatcctaaac caaaaccacc tttttgccgg gttctagcct
    11221 tgtctactcg ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc
    11281 cattatcaga aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt
    11341 caccatcctc ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca
    11401 gaagaagctg atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat
    11461 tattttccga agcaatcatc tcatagtttg tgaaagttgt attaaaaaag ctagttggaa
    11521 cttctgcaag ctctggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg
    11581 ttaaaaaacg catatttcgg acttcttcca aactcgatgc aatgctttca ctcacatcca
    11641 taatggtagc ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg
    11701 gagttcgtaa ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa
    11761 gcaggggcct aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga
    11821 aactagccat cacgaccaca atcaattgct catgcttttg actagcctgc tccaactgac
    11881 tggtattaat caagacagca gcatacttga tattggttga aaccgaacta atattggtct
    11941 ccatcaaaat cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt
    12001 agatatgtcc tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc
    12061 cagaaagaag ttgagtaaag tccttatcaa agagaatgac ttccgtattg gaactgacat
    12121 taggttttat ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt
    12181 ctgttgccct atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa
    12241 gcttatcgtc caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca
    12301 ttgtagaaaa aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg
    12361 cataccatgt ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaag
    12421 gtttgcaaat tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag
    12481 acttcgacaa ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc
    12541 gtcttaggca aaatcacatt ttgattttga aggaaataaa ctagtaaatc gaactctttc
    12601 cccagcaatt cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg
    12661 atattcccat aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga
    12721 atccgcattt taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt
    12781 tcaaatccat gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc
    12841 gtaattccct tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc
    12901 aaatccagca aaatcaagtc atagacacca ctctcagctt cgtagagacc ttcttctcca
    12961 tcaaatacct gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga
    13021 cctaggtcat cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta
    13081 ttataccaaa tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag
    13141 ttttcttttt attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact
    13201 gcagcttttt cacggctaat caagtcaaca cgtgctgcaa tttccttgat tcccataccg
    13261 atgttacggc taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc
    13321 aagcgttgct gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata
    13381 tctcctccaa gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt
    13441 ttctgagttg cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg
    13501 accacaaatt tgtccttcca agaagtaatc aagttttgga tattatccgc atctgtactg
    13561 tatgcaagag ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta
    13621 tcaagataaa gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact
    13681 tgtgaacgaa tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg
    13741 ctagcgactt gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga
    13801 cgaaccaatt catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag
    13861 ttatgacgag ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca
    13921 tcaataaagc gctcaagagc tgaaatcact tgagaaacag ctgaaaccac cagataagac
    13981 tcttccttag caagtttatc aagaactgga agcaagtctg cataagaaat gtgccctgcc
    14041 tcagccaaca aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct
    14101 agctcagcaa gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta
    14161 ttttcagtgt tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccagag
    14221 atttcgatac tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc
    14281 accacccaga gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc
    14341 ttcaagacat cattttcaac tttgacagta agaactgggt aaccaggctg ttccaaccaa
    14401 gaatccatga aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca
    14461 ctaccaatgg tgttgctgta ttggtgtttt tcaaagtagg cgtgcaaacc tttagcaaaa
    14521 tcagcatctc ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg
    14581 atagcgccgt caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac
    14641 tgaacgccat cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct
    14701 tcaaagatat tccagcttgg ttcgatggta tccacacaga cgtattccat catattagcg
    14761 aaactttcat tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat
    14821 tggtgagcca attcatgagc cacaacaagg gcaacttgtt gacggctagc aaatgtagag
    14881 ttttcatcga caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca
    14941 ccagctgaga agtcaggaag ggcgatgtgg agagattgag gaattgggta cttaactcca
    15001 tagtaatctt cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt
    15061 gaaagtggat gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg
    15121 gtcacccctt gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt
    15181 gtctcaaact tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt
    15241 gacaaggcca attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg
    15301 gcttcaggct catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta
    15361 gacaagacct ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg
    15421 ttgtctgtaa ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat
    15481 tcgatatgaa gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact
    15541 tctacagagg tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga
    15601 ccagtgatgg tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa
    15661 tcataatgtt caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc
    15721 tattctaagt ttaagagctg ctgctcttct tcaaacaaac ttattatatc atattttgct
    15781 tgagaaaaaa ggattggaca gcgatttcca aaactttctt ccttctaata gtctacagtg
    15841 ggtagacctt gcgaaattct tctaaaacaa ccttgctttc aggtgtaaaa gtcagcccta
    15901 cttcacccaa acagtctgtg aaaaagtcct catgaacctg gcgccaatag gcaagtgatt
    15961 tgtctccctc accttcctta aaggcatgtt gcgcagaaac ttgattgaag agttcaacag
    16021 aaacctttgt aatttcgaca atgcagacag cctgattttg actatctaaa atgacatcga
    16081 aggtccctac ttggggaagt ggttcgtctt ctagtacata gaggtcatag gctgatgctg
    16141 ttgctgtctt ttctccttta aacaccaaat ccgctaaaag gtctggttca actccaaaag
    16201 cccaagcatc gatttcatct ccgatcaaag gattgatttg cttgtattta ttccacattt
    16261 cttgcggtgt catgggtact cctttgtaat tttttacttt cttcttttat gtgtttaaga
    16321 tgatctggat ggtcaatctc taaatcaaaa atctctggaa tagaactgta gtggataatg
    16381 cacttgatac ccaactgatt cattttttgt atgaaagaag tattcagata gcctgctaca
    16441 gcaaaattaa tcttgttctt tcttgcttta tcctgcatat ctcttagcat atctaacatt
    16501 attggacttt ccatatcatg ccattgactg tttctcatag tcgcaaaaac aaaggaagtc
    16561 aaatcattca ttccaactac aatctttgaa atgcccgttt ccagtatact agataagtca
    16621 aaatacgctg acggtaattc aatcatcgtt ccgactttcc cagtaaagcc atactgacgc
    16681 aatactgtaa tagcttgttt taattggtcg gcatcattga caaaaggaaa gataacagat
    16741 agattggggt tggtttgata aacttctgta acgacatgtg cttcagcctg aaattcatcc
    16801 aaacacgcca gtaaacgcct agttcctcta tagccaaaca agggatgccc ttcgtcaaaa
    16861 aactctttag tccccactaa acaattggct tctgtattcg ttaattcagt aaaacgatac
    16921 caaacttctt catctgagta cagggagcag atagtctcta gataatcttt tacaaattgc
    16981 tgacaacttg ctaatatgat gttttgatta agctctctca acaaatattc cccacgaatc
    17041 atgccgacgt ggtgaaatag ttgaggataa attttttcaa gaattttttc gccactaagg
    17101 gcaagttgat ttctcatcat tcaccttcca attcatgtaa gaagtcttgt ccagttctgg
    17161 aaatcctaat aattcagact taaccttaaa gactaatggc gatgcatttt cttctgtaat
    17221 ctcttgaata tccatccaaa tatatccaag tgaatcattc gcaccatcag acacagcttc
    17281 cgaaatcgta acttgaggtg cactctcatt catttcaaca tcatacaagg ccatgacatg
    17341 gtgaaccata aaatttttta actcttctct gacgaaaaca tcgtagattc gaggattaga
    17401 gtagcttcta acagtaaatc ccgtctcttc cataacttct ctagtcagcg tttccgtcag
    17461 tccttcacca agttgctgac tgcctccagg tagatcatac cgatgttgat aagggcctct
    17521 cgttttttca atgcagagta acttcccatt ttctaagcaa acagcgtaga ctccaaagtg
    17581 ttttttgatt tccatccaac tcctcctact tcaaagacca gccaccatct attgtcaaga
    17641 tttgtccttg catggcgctc gcttttccac ttgctaaaaa aagactaagc tctgctattt
    17701 cctctggctc aatccagcgc ttgattgggg tttcactagc cacccagtca gccaaaccac
    17761 ctggttcaaa atccgcagcg gtcatagctg tcttgactgc tcctggagcg ataccaaaga
    17821 cctgaatccc agcttcagca tagtctagag ccaactgctt ggtgaagcca gccaaggcat
    17881 gcttggatga agtataggcg tgaccacctc cacctgctag gctagaagca atggaacaca
    17941 tattgatgat gattcccttt ttattttcca gcatttgtgt caaataatac cgagtcaact
    18001 ctactggaat aatgtagttg atttcaaaaa tctcttgaat gtcctgcgcc gtttgttcca
    18061 acagtggttt gtaatcatcc aaaactccgg cagtattaca caaaacatcc acctgagggc
    18121 accagtcaaa aataggttcc aagtccaagg tcaaatctct ctgtaaaaag tgaaaatcac
    18181 cctctaagag tggcttttca ccttggtcaa ctccataaac ttgatagccc ttctctaaaa
    18241 agaggcgagc ttgagccaat ccgatccctg aactcactcc tgtaatgagt acacgtttag
    18301 tcatgcactt ctacccaatc cgttgccaaa acatcacaag ctgtcgggct ccacatggaa
    18361 aaaccttctc cttctccaga cacgttgatt aagaaataag gtgtcacttc aagtgcaagc
    18421 ccattttgct cgatggtatc aaagagttgg acatagtttt cagcacctcc ccaaccagtt
    18481 cgtacatatt ttttcttagc ctttaagcca ggtagaattt cttcaaatgt catgtttttc
    18541 tcctttaatg tcaataattc tccccttcat tataacaaaa aaaccgcttt aaaacggctt
    18601 tttgactgtg atttatttac atcttcttct acttacggca aattattccc tgcagcaaga
    18661 taaatttcat accattcttt tcttgttaag ctaaagtttg ccgcttggct aacttctctc
    18721 aagtgcttag gatttgtggt acctacaact gcctgcattt ttgctggata acgcaatatc
    18781 caagaaatgg caatagttga agaggttact ccatatttaa tagctaaacg atcaagtact
    18841 tgatttaaag cttgaaattt ctcatttcca acaaaattcc ctttaaaata cccgaattgt
    18901 aagacagacc atgcttgaat gaccacatcg tgtaattggc aatattcaaa aatgctgcca
    18961 tctcgcatag ctgcttgact atcttccata ttaacatgaa aagctgattc aaatcctgga
    19021 gtaaaagccg cactcaattg tagctgatta acagctaacg gctgcttgac atctttttta
    19081 agcaactcca tcatcatagg attttgatta gaaactccaa aatctcgaac tttaccttgt
    19141 ttataaagga gatcaaaggc ttctgctact tggtcagatt ccatcaaagc atctggtcga
    19201 tgaaggagca agctatctag atgatcaatc ttcaatcttt gcaaaatacc gtctactgat
    19261 tttataatat agtccttaga aaaatcaaaa taggtaaatt cttcaatgcg aatgccacat
    19321 ttggactgaa tccacatctt ttctcttaaa tctggacgat tttttaggac aagacctaac
    19381 agttcttcac aacgaccacg accataaata tcagccaagt cgaaggcatt gattccaaca
    19441 gaaagtgctg tttctacaag ctcttcaact tcttttacag acttatcttt tattctcatc
    19501 attccgagaa caatttctga taattctttg tcatcttgac caagagttat gtatctcatc
    19561 aaatttttct cctttaattt ctaacattct tcccttcatt ataacaaaaa accgctttgc
    19621 aacggctttt tgactatact tcactccatt ttatcttctt aaacccacgg aacaagacaa
    19681 agattccaat aaagaggaca gctaaaggaa taacttttgt aaggaaaaca tttgaaattc
    19741 ccatccactc ataataacgg agcagagaac ccaccacaag atgggcaata atcatactga
    19801 caaatggacg aaagaccgct tctttccaat tccaaataca gataactagc gaaatcgtaa
    19861 agacagacaa actatcccag ggagccggaa tataaaaggc tccttcttgt atgaagcttg
    19921 ccattcctac atatcctaaa acaactaaaa gaactatagt cccaacaaca atgtaagtgc
    19981 caattttcat tttaggagaa tcttggacta aacttcttcg taaaattgtg gccacaagtc
    20041 caaatccaat cagaaaaata agaagttgcc ctaaaaatgt gagcaaattg actgttaaga
    20101 gaggaccttt agaaaaatca cttagtagtt gataataacg taataccgcc aggacaagaa
    20161 ttggcgtcaa aagggactct ttgatagaac tgcgaggtgc tcccttgaga atctctttca
    20221 ttattttttt aggattctta cctagataat cctctgcact catgccatct cgttctgctt
    20281 ctgagaaatc tagcatcatc aaatagatct gctctctgag atagtcttca tcatagagaa
    20341 atccagcaag attaaaactt tcccacaact cctcaaaata cttttgattc tcctcagaaa
    20401 actcatgtag caaagcgctt gtttcttcgt aatacttcat tttcttcatg gtttaacccc
    20461 cattcttaat cccttctact ttttgactca aatcgtccca ttgttgccaa aagactgaga
    20521 cacgctcttc tccttctttc attaatgaaa aatacttccg atctggacca tctggcgacg
    20581 ggcgcatgtc gcctcttatc cattgatttt tttctaactt ttgcaacaaa ggataaatag
    20641 ttcctggaac gatagtatca aatccagcct ctcgcaaagt ctgaaccaac tcataaccat
    20701 accgctcttt ttgaccaatc atatccaaga cacaaccttc aagaacacct tttaatagct
    20761 gagtttcttt catcacttct cccttctaat ctattttgta atacctacta gtaacttcac
    20821 ctatagtata tcacttctac actagtttgt aaagcataat agttaatact cttcgaaaat
    20881 ctcttcaaac cacgtcagcg tcgccctacc gtatgtatgg tt
//
LOCUS       DAVBDG010000033        20571 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBDG010000033 DAVBDG010000000
VERSION     DAVBDG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20571
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.033"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(2..1681)
                     /locus_tag="VQ526_001246"
     CDS             complement(2..1681)
                     /locus_tag="VQ526_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2554971.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     ICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEILILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(1683..1916)
                     /locus_tag="VQ526_001247"
     CDS             complement(1683..1916)
                     /locus_tag="VQ526_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2554972.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRIAARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFKITEF"
     gene            complement(2722..2874)
                     /gene="cibB"
                     /locus_tag="VQ526_001248"
     CDS             complement(2722..2874)
                     /gene="cibB"
                     /locus_tag="VQ526_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2554973.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(2876..3061)
                     /gene="cibA"
                     /locus_tag="VQ526_001249"
     CDS             complement(2876..3061)
                     /gene="cibA"
                     /locus_tag="VQ526_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2554974.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            3239..3922
                     /gene="tsaB"
                     /locus_tag="VQ526_001250"
     CDS             3239..3922
                     /gene="tsaB"
                     /locus_tag="VQ526_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2554975.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQILAEMTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVSLMDARRNNVYVGFYENAKPVMPEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            3919..4356
                     /gene="rimI"
                     /locus_tag="VQ526_001251"
     CDS             3919..4356
                     /gene="rimI"
                     /locus_tag="VQ526_001251"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2554976.1"
                     /translation="MIEIKRIQKHSDLAQAIYAVMIAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            4346..5356
                     /gene="tsaD"
                     /locus_tag="VQ526_001252"
     CDS             4346..5356
                     /gene="tsaD"
                     /locus_tag="VQ526_001252"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2554977.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(5396..6702)
                     /locus_tag="VQ526_001253"
                     /pseudo
     CDS             complement(5396..6702)
                     /locus_tag="VQ526_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7035..7329)
                     /locus_tag="VQ526_001254"
                     /pseudo
     CDS             complement(7035..7329)
                     /locus_tag="VQ526_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7436..7729)
                     /locus_tag="VQ526_001255"
                     /pseudo
     CDS             complement(7436..7729)
                     /locus_tag="VQ526_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            7972..9146
                     /locus_tag="VQ526_001256"
                     /pseudo
     CDS             7972..9146
                     /locus_tag="VQ526_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            9139..10014
                     /locus_tag="VQ526_001257"
                     /pseudo
     CDS             9139..10014
                     /locus_tag="VQ526_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375706.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            10101..11717
                     /locus_tag="VQ526_001258"
     CDS             10101..11717
                     /locus_tag="VQ526_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2554978.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTSQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            11737..12490
                     /locus_tag="VQ526_001259"
                     /pseudo
     CDS             11737..12490
                     /locus_tag="VQ526_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12558..14058
                     /locus_tag="VQ526_001260"
                     /pseudo
     CDS             12558..14058
                     /locus_tag="VQ526_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            14070..14819
                     /locus_tag="VQ526_001261"
     CDS             14070..14819
                     /locus_tag="VQ526_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2554979.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYEYLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPCVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISLILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            14832..16004
                     /locus_tag="VQ526_001262"
                     /pseudo
     CDS             14832..16004
                     /locus_tag="VQ526_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            16399..17262
                     /locus_tag="VQ526_001263"
     CDS             16399..17262
                     /locus_tag="VQ526_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2554980.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTALFE
                     LRLQQYKKLID"
     gene            17548..17781
                     /locus_tag="VQ526_001264"
     CDS             17548..17781
                     /locus_tag="VQ526_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2554981.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            17803..18480
                     /locus_tag="VQ526_001265"
     CDS             17803..18480
                     /locus_tag="VQ526_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2554982.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            18492..19157
                     /locus_tag="VQ526_001266"
     CDS             18492..19157
                     /locus_tag="VQ526_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2554983.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            19229..20455
                     /locus_tag="VQ526_001267"
     CDS             19229..20455
                     /locus_tag="VQ526_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2554984.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         6444 a         3208 c         4155 g         6764 t
ORIGIN      
        1 attattcttc atctggtgtg aggatcatcg ggatgatgat cggttcacgt tcggtatttt
       61 catagaggaa ggggcgaata gcgttgacaa tggcaccatt gacagattgc acgctagcat
      121 ccttattttt cagtgcgata cgaatggcat tgaagaggat acgctggctt tggcgaatca
      181 agtcgccaga ctctctcatg tagacaaagc ctcggctgag gatgtctgga ccagatagaa
      241 tcatctgcga tttgaagtca acagttgcga ctgccagaac gacaccgtct tcagatagat
      301 cgcgacgatc tttgaggaca gctgcgccaa tttcaccgat acgatttcca tcgacataga
      361 tatcttgggc gttgaaatga cctgcgatac gagctgagtc agcagtaagg gcaagcacat
      421 cgccattgct catgataaag atattgtcct tctcaacacc agtatccact gctagtccag
      481 cgtggacttt ttgcatgcgg tattcaccgt ggacaggcat gaagtatttt ggcttaatca
      541 agcggagcat gagtttttgc tcttgctgac caccgtgtcc agatgtatgg atattgttca
      601 ctttaccgtg gataacttcg acaccagctt cagaaataat gttaatcagc ttgttgacgc
      661 tagtagtgtt tccagggatg ggactagaag agaagataac ggtatcgcct ggttggagtt
      721 gtacctgacg gtgggttccg ttggcgatac gagagagggc tgccataggt tcaccttgac
      781 tacctgtaca gaggatcaga atctcacctg caggatagtc tttgatttca tttggctcaa
      841 taaaggttcc cttaggagct ttgatgtagc caagatcgat tccgttgaca atggcctttt
      901 ccatagagcg accaaagacc gcaatcttgc gcccagtctt aacagcagct tccgttgctt
      961 gttggagacg gaagatattt gaggcgaagg atgcaaagat gatacgtccc tcgatacctt
     1021 ggataatctt catgatggac tggccgacaa ccttttcaga gttggtaaag gttggaacct
     1081 cagcattggt cgagtcagag agcaagcaaa gcactccatc ttcacctagg gctgccatac
     1141 ggtgcaagtc cgcaggttct ccaactggag taaagtcgaa cttaaagtca cctgtacaga
     1201 tgattttccc ttgaggagta tggatgacaa ttcccaaagg ttctggaata gagtgagtcg
     1261 ttctaaagaa agttgccttg agatttttaa aggtcaactc ggtgttgtgg ttgatttcgt
     1321 aaagtttggc gttgcgcaag aggccgtgtt cttcgagttt cccacggatc aaagccaagg
     1381 caagcggtcc agcataaata gggacatttg cttgcttgag taggaacgga atcccaccaa
     1441 tgtggtcctc gtgtccgtgt gtgattaaaa cagccttgac gcggtcgata ttgtctacga
     1501 tgtaagagta gtcaggaatg acatagtcga taccaagcaa gtcatcttct gggaatttaa
     1561 tcccagcatc gacgatgata atctcgtctt ggtattcaat tccgtaagtg tttttcccga
     1621 tttctcctag accaccgatg gcaaaaacgc cgacttcttc aggtttaaga gtgtaggcca
     1681 tattagaact ccgtaatttt gaaggcgcca gtttcttttt cgtaatcgag caatttgtca
     1741 gacaagagtt cgatatattc gatattgtac tctgggcgat tttcttcgac aagttggcga
     1801 gcagcgatac gaccctcaag ttctgagctg gcatcgatgt ctaggtaaag cgcgcgtgtt
     1861 gtttcacgac gtgggctacg ttcttttgtt tcttgataaa aaactttgta aatcatatag
     1921 ttcctttcta tttacaggtc agcttattcc aaacttttag cagagatttg cttgatttca
     1981 ttccattttg tgtctagatt gaccttgatt cgttacaatt atttcaatta taccacaaaa
     2041 tgaaaaatta gtaaaagacg gaaacgagaa agttagggga cgatttcttt ggtaagcgtt
     2101 tgcaaaaaca aagaaaatgt attatgttga taacggaagt gaaatttatt gcttgtcata
     2161 attccatata gattttaagt caaggaggtt gccctatgta taacttattt gcttttgcta
     2221 tcggagttcg tttgctagct tttatcgttt ttgttgcttg cgtttatgga ggaaatcttc
     2281 tatttgccaa actggaacga tgacaaacca agttcttgag gaaaattacc tttgtgactc
     2341 tctactcgct ttttctcctc ctcgtgacct atgtcgtttg gtttgtattt tactttggat
     2401 tgccctgctt atgctggggt tttttgtttg gggatgtaga gatagtagat ttacgacacg
     2461 aaaattccac cccatctatc aagatgaggt ggtctttgca tttttctaac atgacatgac
     2521 aatggaattt tcaaagctta ttatttttat gggagtcatt gttctgctca aaaatagagt
     2581 tgaagcataa aaataaaaca atacaaacag ataaatcgaa tggtaaaggg atagtagtaa
     2641 tgttcaaatc attgagaatg ctgagcaaac tgtaagagat gaagacaata aaatttatcc
     2701 ttctaaaaat gtgtttctta tctagcatga cttaccaaac tttttacgaa ggtcttttcc
     2761 aatggtataa cctgccataa ataaagcgat accaacacct acaccaaagc cacccttgat
     2821 ttcttgcaat tccttattag aaatttcacg atagttgttc aaatttttca tcatattaat
     2881 ctcctcctaa aaaataacca gctgccaaac cagaacctat aagtgctgtt ccacctgcaa
     2941 tagctttcca agatactgct actccaaaga taaccaaggg agcgagaccg ccatcaatat
     3001 ctgataattc attttcagat aaggataaaa attgattgtc aagaatgtca aaatttgtca
     3061 ttgaattttc ctcctttttt cttgttcact tttatattcg gaaaagaaaa ggaaaagtga
     3121 gcattttttt aaattatttt tagttaagtg agggcactct tgtctggggg caaagaaaat
     3181 tcccatgtgc cagcttttgt agtataatag taagcagaca aaaagatagg aatgtgttat
     3241 gaaagtatta gcttttgata cgtccagcaa ggctctttct ctcgctattt tagaggataa
     3301 gcagattctt gccgagatga cgattaatat taagaaaaat cacagtatta ccctcatgcc
     3361 tgccatcgat tttttgatgg caagtttgga ctggacaccc aaggatttgg accgaatcgt
     3421 ggtggctgaa gggccaggta gctacacagg cttgcgaatt gcggtagcca ctgccaagac
     3481 tttggctcat actctgaaca tcgagttagt tggtatgtct agcctcttgg ctctggtacc
     3541 ctaccaacaa gaaggcttgt tcgtctcttt gatggatgcg cgtcgcaaca atgtttatgt
     3601 aggattttat gaaaatgcta aacccgtcat gccagaagct cacttaccct ttgagcgagt
     3661 gattgagtta atcaagggtg ctagtcaggt tacctttgtc ggagaagttg gcccctttgt
     3721 tgagcagatt caagaatact tgccaaggac taatttcaaa gaaaccttgc caaatgcagc
     3781 taatctagct ctatgggcct gggacaagga agcagactcc ttgcatgatt ttgtgccgaa
     3841 ctacctcaag cgtgtcgaag ctgaggaaaa ctggctcaag aatcacaccg agtctggcga
     3901 gtcttacatt aaacgcttat gatagaaatc aaacgaatcc aaaaacattc tgacttggct
     3961 caagccatct acgctgttat gatagctgtt tacccagtca gtccttggac gctggaacaa
     4021 atccaagcag acctgtccca agaccaaact tggtatgctt tggcttatga tggggcagaa
     4081 gtgattggat ttctagctgt gcaggagaat ctttttgaag cagaagtcct gcaaatcgct
     4141 gtcaaaggag cttatcaggg tcagggaatt gcgtcagcct tgtttgctca attgccgaca
     4201 gacaaggaaa ttttcctcga agtcagacag tcaaatcaac gagcgcaagc attttacaag
     4261 aaagaaaaga tggcagttat cgctgagcga aaggcctact accatgaccc agtcgaggac
     4321 gccattatca tgaagagaga aatagatgaa ggatagatat attttagcat ttgagacatc
     4381 ctgtgatgag accagtgtcg ccgtcttgaa aaacgacgat gagctcttgt ccaatgtcat
     4441 tgctagtcaa attgagagtc acaaacgttt tggtggcgta gtgcccgaag tagccagtcg
     4501 tcaccatgtc gaggtcatta cagcctgtat cgaggaggca ttggcagaag cagggattac
     4561 cgaagaggac gtgacagctg ttgcggttac ctacggacca ggcttggtcg gagccttgct
     4621 agttggtttg tcagctgcca aggcctttgc ttgggctcac ggacttccac tgattcctgt
     4681 taatcacatg gctgggcacc tcatggcagc tcagagtgtg gagcctttgg agtttccctt
     4741 gctagccctt ttagtcagtg gtgggcacac agagttggtc tatgtttctg aggctggcga
     4801 ttacaagatt gttggggaga cacgagacga tgcagttggg gaggcttatg acaaggtcgg
     4861 tcgtgtcatg ggcttgacct atcctgcagg tcgtgagatt gacgagctgg ctcatcaggg
     4921 gcaggatatt tatgatttcc cccgtgccat gattaaggaa gataatctgg agttctcctt
     4981 ctcaggtttg aaatctgcct ttatcaatct tcatcacaat gccgagcaaa agggagaaag
     5041 cctgtctaca gaagatttgt gtgcttcctt ccaagcagca gttatggaca ttctcatggc
     5101 aaaaaccaag aaggctttgg agaaatatcc tgttaaaacc ctagttgtgg caggtggtgt
     5161 ggcagccaat aaaggtctca gagaacgcct agcagccgaa atcacagatg tcaaggttat
     5221 catcccccct ctgcgactct gcggagacaa tgcaggtatg attgcctatg ccagcgtcag
     5281 cgagtggaac aaagaaaact tcgcaggctg ggacctcaat gccaaaccaa gtcttgcctt
     5341 tgataccatg gaataaagag tggctctttg tcaagtgtag tgggtaggcg aaaagctaca
     5401 atctggagag tacgaaattc gtactctctt ttttgatgtt caaagtcatc aaaatccgtt
     5461 ttttaaaatt gttaaagttc cgaaaaccaa aggctttgcg cttgataacc ttaataaaat
     5521 tattggtcgc ttcaagttta gcgttggaat aaggcagctt aagtgcgttg ataatcttat
     5581 ctctatccct taaaaaagtc caaaagaccg tttgaaaaag cggatgaacc gttggcagtt
     5641 cttgctcaat caatccaaag aaatggttgg cattcttctc ttgaaaatga aagagcagga
     5701 gttgatagag ttcgtagtga acctgtaatc cctcagagta agaaagcagc ttatctcgta
     5761 cttcccgatg ggttaagtgc attcgaaacg tgggctgata gaatcgtttc tgacagagct
     5821 tgaagctatc ctggtgtagt aaatcagtcc agtggactga tttagcccga gcctagaaac
     5881 gaaaaagcga gggttccaaa agcgcttgag cgcccgatac tccaaggatt ttcggtcaaa
     5941 ttggttcatg atttggattc gtactcggtt catagctcgg ctcagatgtt ggacaatgtg
     6001 gaggcggtca agaacaatct tcgcctttgg aaagagttgc ttagcgagcc gataataagg
     6061 gctgtacatg tccatggtga tgacctcgac cagctcccga acctctctct gatagcgttg
     6121 gaaatggttt cgaatcaccg catgagttcg cccgtctaaa attgcgagga tggatttgga
     6181 ctcaaaatct tgggcaatga agctcatttt gctctttttg aagctatact catcccaact
     6241 cataactttt ggcaacttgg tccaatcggt ttcaaactta aattccctca gttttcggat
     6301 gacggtggaa gttgagaccg ccaatctgtg ggcaatctca gtcatagact gcttttcaag
     6361 gaggagctga gcgatttttt gtctcaccat gttggaaatc tggcaatttt tcttgacaat
     6421 ggatgtttga gaaacgacca ccttaaggca attcttgcac tgaaagcggc gctttttgag
     6481 atgcagtacc gtgggtaaaa cctgacagtc gagaagcgga attttagagg ctttctggaa
     6541 gtcgtatttg atcatcttcc cttgacaatg aggacaagga ggagcggggg aatccaactt
     6601 tgcctgaacg actagatggg tttggtattt cagaacagac aagattttga tatttttgtc
     6661 ttccaatccg agcaaatctg tggtattctt aagttgttcc atatgaaccc tttctaatga
     6721 gttggttaga cgcttttcat tataggtcat atggggcttt ttttctacaa gaaacgaccc
     6781 tataattcct ggggtgggat tacccactac agaaattata gagccaaagc attccaaagt
     6841 cttgtctgac tctggaatgt tttctgttca cagttgtttt gtaactgcct aataagtaac
     6901 cgcgctataa cgaggtatct accgagattg tcaattctcc agtatactgt tataaaaaac
     6961 aaaactggag gattttttta tgtcccccca tcgtcccatc gactgttccg tagctgccca
     7021 ataacggagt ataatcagct gaaacaagaa taggacaaaa gtgcctcaag aaaagcatcg
     7081 taatttgaca atattattct gagatgtttt tgatgtaagc ccatattttc tcaatgggat
     7141 tatactcggg tgaatagata ggaagtggta acagtctatg ccctgctcct tacacatgtt
     7201 cattctgtga aaccttgcat tgtccataat gataagggat ggtgtatcta aagtaggtag
     7261 taagaatttt tggaagcaag cttcgaaaaa gtcactcgtc atcgtatctt tataggtcat
     7321 cggagccata gtatattgaa acaaaaaagg gcattagtac ttctataaca gtatcgcaat
     7381 ctctggtgtg cgttgtctcg atcttaattc aaataattat atcttcttcc agagagacta
     7441 actgcctgct caaggctcga tcatattcga gataaaaata ggtcttaacc cctgtctcat
     7501 aaatataaat aggagtcagg tagcttaagt gattcaattc tttaaggaac cgatttactt
     7561 tttctgggtc ttgttcgcag taggtacggc tcttttttag actatatccc atagccttta
     7621 gagcgtaatg aatagttgtt ggaggacagt tgaattcagc agctatttca gtcaaataag
     7681 catcgggatg agtttcaata tagatcttta atttatctct atcaactttt tcaattaata
     7741 gtggaagttt tcttaaagtt agtgtattga aacgttttta tacagtgcta aaatttgaag
     7801 tgtagtgcag atatatttgt aatagtatta tagaaattct ttgaagaagg tcgagacaag
     7861 agaaaatagg tatctttgag atataagcat gatttactat aaaattattg agttattcat
     7921 aaaaatttct ctatcaaata tcataatgcg tcgtttaagg agatggttac aatgcaatat
     7981 agttgtggaa aaataaatat taatatccct gatggatatg gagacattaa ggatatagtg
     8041 ttttcggctc atattatagt taggtataat aatggacatt gtggaggtat agatccgcat
     8101 ataattggac tttgtaaaaa gcagatcaga aggatgtctc tatatcctat tttgataatc
     8161 gtatctagag attcaaaggt tattgatgat tataaaaatt tagatattgc ctatgttgat
     8221 tgtactcaat gttcaaataa ttttgaaact gccttacacg ttaaaaatat tttaaaatta
     8281 ttaaaaatcc aacttataca ctgtcatggc tactcaacta attatttttt atatatgtta
     8341 aaaaaacttg acaagaatgg atttggaaag gttaagacag tgataacatg ccatggatgg
     8401 gttgaatata acttaaaaaa gaaattcttg acttattttg atttttggac atattctatg
     8461 ggagatgctt ttatttgtgt atctgaaact atgaaaaaaa gattggagag tataataaaa
     8521 aaataagaag atagttgcaa taaataacgg aattaatgta tcaaatagcg atttagatgt
     8581 agtaggtgtt caagacttta aaaaagaatt ttgtattcca aataacaaaa aaatcatttg
     8641 ttatgttgaa aggttggatc cagaaaaaag gccggataga ttccttgaat ttgcagaaaa
     8701 attattttta gtcagagaag atgtgatttt tattatggct ggaaatggta gtatgtgggc
     8761 tgctttgaaa gaaaaaattt gccatttgaa atgtagagat aatttcaggt tacttggtga
     8821 aatctatcca gctactatag tttaccaaat atcggattta ttgtatattc cttctgatac
     8881 agaaggaata cctatgtgtg ttttagagtc aatgtcacaa ggaactccgg ttttagctag
     8941 taatgttggc gggttaagtg aaattattga acatagggtt gatggatttt tatttgagaa
     9001 ggaagatgtt gagggagtgt gtgcttgtgc taatttttta ctcaatgatt ctgagtattt
     9061 gaaatatata ggtgagaata gtaaatcaaa aataagaaaa catgagagta tatgatgaat
     9121 tattagagaa gagtagtcat ggataggaat atagatcaag aattagtaag tattataata
     9181 ccaacacata ataggtatga atctctgatt agagctgtga aaagttgttt acatcaaagt
     9241 tataaaaata tcgaagttat aattattgat gataattata gtaatgtaaa tttaagaaat
     9301 aaaattatcc atcaatttgg ctataccaat catagaatta agttaatttt aagtaatgaa
     9361 gatttaggtg caactaatgc cagaaacata ggtatcaaaa attctagagg taagtatata
     9421 tcatttttag acgatgatga tgaatatatg ccagattgaa ttttaaagtt gatggcttgt
     9481 tttaaaaaga gtagaatgaa gaatttagct ttagtttata gttatggaat aataatttat
     9541 ccaaatggta cacgagaaga ggagaagacc gattttgttg gaaatccctt gtttgttcaa
     9601 atggttcaca atatagcagg tacgtcattt tggttgtgta aaaaagaggt gctagaatta
     9661 attaatggtt ttgagaaaat agattcacat caggacggtg ttgttttatt aaaactactt
     9721 gctcaaggat accaaattga tatagtgcga gaattcttgg tgaattacta cgctcacagt
     9781 aaagaaaacg gtatcactgg agtgacacaa aaaacaatta atgcagatga agaatattat
     9841 aattactgta ggaaatattt taatttattg agtttcaacg agagaatatt ggttacaaag
     9901 aaatattatt ctttaaacat aaagcggtta ctattaatag gagacaaatg caaggcttta
     9961 aaagtaatca agaaggcaag agaagaaaaa atttttaacg aatttctttt ttgaaatata
    10021 tgttattata tcattttggt tttttctatt gtatatatga caactatgtt caattaaaat
    10081 ttagaaagtg agaaactatt gtgtatacta ttataaaatc aaatataaaa aaatttagtt
    10141 tattaacgat atttattgtt gctggtcaat tattgctaat ttatgcagca actattaatg
    10201 ctctggtgtt gaatgaatta attgcgatga atttagagcg gtttttgaaa ttgtcaatct
    10261 accaaatgat tgtctggtgt gggataatat tccttgactg ggtagtgaaa aattatcagg
    10321 ttgaagtgat ccaagagttt aatctagaga ttcgaaatag agttgccaca gacatctcta
    10381 actctaccta tcaagaattt catagtaaat catcaggaac atatctttcg tggctaaata
    10441 atgatgttca gactttaaat gatcaggctt ttaaacaact ttttttagta ataaaaggaa
    10501 tttctggtac tatatttgca gttgtgactc ttaatcacta tcattggtca ttgactgtag
    10561 ccaccttgtt ttcattaatg attatgctac ttgtaccaaa aatctttgca tcgaaaatgc
    10621 gagaagttag tctaaattta actagccaaa atgaagcttt tttaaaatct agtgagacta
    10681 tattgaatgg atttgatgtg ttagcgtcct tgaatctttt atatgtattg cctaagaaaa
    10741 ttaaagaagc aggaatttta ttaaagatgg ttatacaaag aaagacaact gtagaaacgt
    10801 tagcaggcgc tattagcttc tttctcaata ttttttttca gatatctctc gtttttttaa
    10861 caggctatct tgcaataaaa ggaatagtga aaattggtac tattgaagca ataggagcac
    10921 taacaggtgt tatttttaca gcgctaggtg aattaggagg tcaattatcc tctattattg
    10981 gtacgaagcc tattttttta aaattgtatt caattaatcc aattgagtca aataaaatga
    11041 atgatatcga accaaatgag gtgaatagag attttccgtt atatgaagca aaaaatattt
    11101 gctataagta tggagataaa gaaatattaa aaaacttaaa tttttgtttt caacgtaatg
    11161 aaaagtattt aattttaggt gaaagtggaa gcgggaaatc tacattatta aaattattga
    11221 atggcttttt gagagattat agtggagaat tgcgattctg cggggatgat ataaaaaaaa
    11281 cctcctattt aaatatggtt tcgaatgttc tatatgtaga tcaaaaagct tatttgtttg
    11341 aaggtacgat tagagataat attttattgg aagaaaatta tactgatgaa gaaatactac
    11401 agtctttaga gcaagttggt ttgagtgtaa aagattttcc taataacatt ttagattatt
    11461 atgttggtga tgatgggaga ttactgtcag gagggcagaa acaaaaaatt actttagcta
    11521 gagggctaat tagaaataag aaaatagtat taattgacga gggaacttct gctatcgata
    11581 ggagaacttc gttagcgatt gaacgtaaga tattagatag agaggatttg actgtcatta
    11641 ttgttaccca tgctccgcat ccggaactta aacaatattt tactaagata tatcaatttc
    11701 caaaggattt tatttaagaa tataggggag ggaaacatga gattctattg tgaagcttat
    11761 gaggtaatta ctgaagagaa aaaagtatca tttttttatg tagatgaaaa acccttaagg
    11821 tatctgttat ttgactatat atttctacta gttctagcta aaaaaatatc ccaatcttag
    11881 atggagtttc caataagcaa gttgctccaa cgttgctgca tcacttttct ttggaaattg
    11941 aaaagaattt tatcgatttt tgctataaaa atatggatct catcctaaaa actcaaagta
    12001 tatctttgtg tcatagagaa gagcttatta ttgtagatgt ttcagatgac tctaaatttg
    12061 gtgtttttta caagcgtttt agaacactgg ttgataaaaa taacggaaaa tatgtttgtg
    12121 tttataattt cagtcgtgtt tcagatatac tgcaaaactg gaaaaattat tgcaatcgaa
    12181 aattttcggt tacgtttact gaaagccaat ttgaattatt taagctttta tataatcaaa
    12241 aaaattttaa aacaatttcg ttgctatttg gaaaaaaatt gttgctggag gaattatcta
    12301 ttatagcgat cttactaata tagaatactt ttgtattttt tggtgggata gtatgtttgg
    12361 gaaagattct attggaaaat acgtatatgt agaagagatt agtagatgtc attttttaga
    12421 tagaaattat tctttttgtt atggtttaca agattataaa agtaaactta ttaaatattt
    12481 tttagaatag ttaatctatt cgagagagat aaagaaatat ggaaaatact ttattgaata
    12541 tcacaggaag cttcgatatg gaaactagga atctaattag ttattcttta actgatatct
    12601 ttgaaacaga taaaataaga attgaacttc ttggagaaat ttattataaa aatataaagt
    12661 tggaattgca tgaattcgct ggattgtata agatttatgg aattagctta ataaagaata
    12721 ttaccggaat gtttttaatt ataatttttg atacgaatac aaaagaatta aaaatttttc
    12781 aagatataac gacttcatac tttaatttat actatactgt ttatggaggt gtgttctata
    12841 tatagtacat ctttaaagaa agtgatgaag ttatctcatg ttcctgttat cttaaataac
    12901 aaaaaaatac aagaatttat gagaaatgga tttattttag attcaaatac tttagtaacc
    12961 gaaattaata aattagaata tttctcttac atttctgtaa ataatactct tcgtatatgt
    13021 ggtattgatt ataatgattc aaacaatttt actaaagaac aagtattaaa gaattgggac
    13081 tctatgctac gtgaatctat attaagagtg tatagtgaag caggagaggc taatattaca
    13141 ctatcatctg gttttgattc aaattatata ttatatacat tagcgaatta tacgaattct
    13201 tcaattaatg cattttgcat aggaggagaa aaaggaatta atgagattcc tgaagtaact
    13261 aaaattgcaa aattttatgg tataaattta ttagttgata ctgtaatgag ccaagattta
    13321 gaatattttc ccgatatagt atggagattg gaaggaagtc tctttgaatg tggtgtcata
    13381 ctgcagtact atttaggcag gttgttgttc caaaaaggga acacaagtat tttttgtggc
    13441 gagtcagcgg atgaaataat gacttttaag tatcattctg tgaactacaa tcaattttgt
    13501 aatgataaac agaagtcagt atattttagt tattccgact atccatttta tgttacaaat
    13561 tcaattgttt taaagaaaaa taacctactt ttgcatagtt ttggcattca tcctagatat
    13621 ccatataaaa tgtctgagat tgttgaaatg tctaaaaaaa tatcaaatct aaacgataaa
    13681 aaagaatttc acaaaaagaa ctgtgagttg aggtttcaag atagtgttct tgataacata
    13741 aatagtgtcc cgggtacaac tcatttattt agttgtttaa atattcaaac attagtaaaa
    13801 attattttat atatttttcg atacaacagt tgtatgaaaa tatttaattt tagagataaa
    13861 gagcttatct tttttaataa gattgtaaat gggctgatac aaaatattga ggagaattta
    13921 gaggatgata ttgaaaggat attgaaatat ttatatatat gtttatttaa cgaaatattc
    13981 attattaaga ataaggtgaa tttttttgat gatgtggaat ttaatcagac acttagtgag
    14041 tttttagata aattgtagag ggaatggtta tgagtaacaa tactaagtat atctttttat
    14101 ctccacattt agatgacgca attttttcct gtggtgatta tatttcaaaa ttaacttcag
    14161 aaggagaaat tgtacttgtt attactatat tttctggtta ccctttgagc caacagctac
    14221 agccctctgc taagcaattt cataagttgt gtaaccttgg gaaatatccg attgaagaaa
    14281 ggaagaaaga agacaggtta gcttgtgaac gtctacaatg tgattttaga cacttatcgt
    14341 actatgaata tctctatagg aaagatagaa atggcaattt tctataccgt catatctata
    14401 gtgaattaaa aaatgaggac acattaaaga atgatatcat aaaagagctg ttaatgcatt
    14461 tagacgacaa atgtgtcgta tattgtccgc tttcacttgg tgaccatgtt gatcatgttt
    14521 ttgtgaatag tattggaagg gcacttgaat ttatgcgtta taaagtaata tattatgagg
    14581 acttcccatg tgtaagtgat agtagtatgg ttagttatat gggaaaaact aaagaattaa
    14641 aaatgtatca agaggaactt gatgaaaaac attatattga tagaatatca ttaatattgt
    14701 gctataaatc tcagattttg ataatctgga aatcagttga aaaactttta aacaatatta
    14761 aagagttgta tttaaggaat ggggcagctt attctattag attttggatt aagaaataag
    14821 gaagtagttt tatgaatata gcagtaattg gtttggggca tgttgggctg gcctacgcgt
    14881 tactatttgc atctaaatat aaagttgttg catatgatat agattctgta aaaataaata
    14941 atttaaaaaa gggcattctt ccatctaaaa atgaagagct tatgaagttt ttttgcgaga
    15001 ataacttaaa tattactttt tttgatacat tttctgaaat taaaaataat attgattatt
    15061 atattattgc gcttccgaca gattatgatg agaaaattgg tagttttaat acatatgaaa
    15121 tcgaacaaac ggtatcgaag attctgaggg taaaacctaa tggaaagatt atttgaaagt
    15181 caacagttcc gatcggcttt tcaaacaaat taaaaaggct gtttgataca aaaaatatca
    15241 tttttgtccc tgaatttttg agagaaggtt gttctatata tgataattta tatccaagtc
    15301 gcatagttgt tggagatgag acagttgaag gaagaaaaat tgcagagttg ttcctttcga
    15361 ttagtactca tagtattgcc aatattaaaa atgttatgtt agtttctcct actgaagcag
    15421 aagcaattaa gcttttttct aacacattct tagctctccg tgttgctttt tttaatgaac
    15481 tagatttttt tgctgagagg agaagtttaa atgctgaagt tgtaataaaa ggtgtttgtt
    15541 tagatccaag aattggaaat ttttataata atccttcttt tggatttgga ggatattgtc
    15601 ttcccaaaga tactaaacaa ttaaaaaaag aatttataga aataaatgcc ccagtgatag
    15661 aagcgattga tatttcaaac acaaatagaa aacagttcat agttaaacaa atattggaac
    15721 gaaagccaaa gatagtagga atatataaat tggggatgaa atataattca gataattaca
    15781 aagagtcagc tattttaagt ataattaatg aactattgat tgtcggtata aaaattttag
    15841 tatatgaacc aaatttaaac gttagtatag ataacgtaat ttttgaaaag aattttgaac
    15901 tatttacaaa acaaagtgat ctaattgttg caaatagatg ggataggggc cttgaagctt
    15961 ataaagataa agtttacaca agggggatct ggataagaga ttaactattg atattaaaaa
    16021 ggcaataatt tataaaattt gtacaaaaaa atgttaaatt ttcacatttt catagtatac
    16081 tgtttagtag aaagtaggtt ctttgtaacc gctccattat aaaatccttt cactcttccg
    16141 gtatactatt aggaaaaaca taactggagg atattttgag ctcacacctc atcgttccag
    16201 tggctgttcc ccaagctcgt gtactgccca aaaaagttag acattctatt tagaacgagg
    16261 attgagttct gtattgtaca aggctcggtc cttttagagt cagcttaagg ctggcttttt
    16321 caatcacaaa agtgtcagaa tgttttgaca aatgaacaca aataatgata taatatgcaa
    16381 aagctaggag gtggtaggat gattgaaaaa atggaactgg gggaatttta caaggaatta
    16441 cgtttggcga gaaagcttaa gcagacagat gtggcttgtg agggtctaac agcttctcag
    16501 ttgtccaagt ttgaactagg gcagtctatg ctgtctgcgg ataagttgat tctagctatc
    16561 caaggtataa atgtgacctt tgatgagttt gggcacaagc tcaacaacta tcaagaatct
    16621 ccacatatgc gaatcggtcg aaaggttgtg aatcgctttg cccatcagga tatagctgct
    16681 ttagagcagc tattggagga agtcgatcaa gaacagatgg cgcagaccta tcgtcgtttg
    16741 aatgccatcg tgattaaaga tgccatccat tcactaaata aaagctaccc actagcagag
    16801 gaggatagcg agtttttgac cacctatctc tatgctatcg agtcttggac ctggtttgaa
    16861 ctctatctct tttgcaatac catgcccttc ttgagcaatc aagatctgat ttttttatca
    16921 acctccttac tcgaaaaatc caaagaattt aaagagttag tacacaatcg attgtatatg
    16981 aagcaaggac tcttaaatat cttatcagaa ctcatggagc gaaaactttt ctcctacatc
    17041 ccaatcttcg aagccgagtt ggagaggatg ttacgaccgt atgatgtttt tgaaaaagta
    17101 tcgtggcaat ttttaaagaa gatgagtgtc tttcttcaaa caaagggaag caatcaaaaa
    17161 gagattgaac gctttatcca atctctgcaa gtattagaaa atccacaatt gacagccctt
    17221 tttgaattgc gtttgcagca atacaaaaag cttatcgatt agttagaaat ggcaaaataa
    17281 taataatctc agcaacgatt ttcaataatg ttaaaatttg tttcttcata tttctccact
    17341 cctcattagt agtaacttta ttataccaga gaagtaatca aattcaccaa aatcgcaaat
    17401 ctgaaatttc tataagaaaa aatatcaaat atgcgatttt ctaaaataag ccaattttcg
    17461 tgttatactg tacttgtaaa gcacttgaag caaatcctag gtcgcagaag tggtttacaa
    17521 atgaagataa ttgaaggagt gtaaaagatg ttaaatttac aatttgcaga aacaatggaa
    17581 ttgacagaag ctgagttgca agatgttaga ggaggcaacc tcgtaaattc tatgggaggt
    17641 ggtggaagaa gtggtatatc tggttggggc gtcccaggaa tttaccctgg ttggggcaat
    17701 caagggatga gtcctaatcg tggagctttt gattggacta tagatttagc agatggattg
    17761 tttggccgcc gtagaagata ggctcttata ggagataata ggatgaagaa aatgaaagaa
    17821 gtgaaatttc acctagcaac tggcttgttg attttgactt attacctcat ttttaatgtc
    17881 acatcagact tagattttat ggttgctttg tctgataata tgtactatgt gttccaagtt
    17941 ttattggtac ttattctggg aactatagct acaattgctt ttgtcaaaag tgaacattgg
    18001 aaagagtgtg gtcgtttcca gtttcgttgg tcttacctgg gtgttttcct tctttctttt
    18061 ttcttattgt tcgtgtgggc aaatctaaca acctatatct ttcctcgtac ccagaatgga
    18121 agtacagtcg tagaagtagc caccaattta acaggaatta gctattttgt tacgcgaata
    18181 ctttatacta gtattatcgc acctgtatca gaagaggttg tttgtcgtgg tcttttgatg
    18241 acaagtttat ctaaagttaa gagatactat cttgatgttc ttgtttccgc tgccatattt
    18301 ggcgctatgc atgttttgca gtatggttgg ataacgacag atttcataaa gtattttgga
    18361 atgggcttga tattttgtat gatgtttcgc tacacccgat ccatctattg ggctatagct
    18421 ctgcatgctt catggaatag tttcttactc attgttactt tattggtatt tggatattag
    18481 aaaggtgata tatgaaaaag ataatctcac accgctactt tattatagtt tttctactag
    18541 ttattgctga ccagaagttc agtgttttag ttttacgcag cgaccttgtt gctgggctat
    18601 ctgactttgc ctattatctg tcggatatga tgttgaattt tcttgtggtt ttatttgctc
    18661 ttattgctat gatttggtcg gggaaatggc agaaaatcaa tagtagaaag tttaaaggat
    18721 cctatctttt ctattccttc ctagctctgc ttgcttttgt tgcttggaat tttgtcacct
    18781 tttttctttt cccacctact cgaaatgaaa tttcttatca acatgctgct cctactttta
    18841 caggagctac ggcatttttg atgtattttt tttatcctgt gattgcaggt cccatttttg
    18901 aagacatgat ttatcgtgga ttagtgatga ctgctctgga aaaaggaaag aaatgggggc
    18961 tagatgtact tggttccgct gttttatttg gggtctcgca cattagtaat cacggttggg
    19021 tcttgacaga ctttgtcttc tatatgggtg gaggtcttat atttgcagtc ttatttagaa
    19081 tgacaaagtc aatttattgg cctattggac tgcatatagt ctacaatggc attggtcagc
    19141 ttttgatgtt actgtaattt tggttgttag tagtatcgcg gtctatcctt ggggaaagat
    19201 tcccatttga acttaagaag gagttgtgat gaaagtattt cttcaaaata gagattttag
    19261 gcaattaact atcaaccagt ggatttcaac gcttggggat acgatttttt atctggcctt
    19321 tctgaattat gtggcagatg cgtcttttgc acctttggcg attttactca ttacgatttc
    19381 agaaaccctt ccccaaattt tacaaatctt tctgggagtt ttggcagatt ttcaacatca
    19441 tcgtgtcttg aaatacacag tcattagttt tgcaaaattt ttgctttact ctatcgtttc
    19501 tttatcactt tcagggcagt ctttctcctt gttgctagta gcatttattt gtctgcttaa
    19561 cctcttgtct gacacattga gttacttttc aggagccatg ctcactccga ttttcattag
    19621 aattattggg caagaccatc tggcagaagc tattggattt aaacagtcaa ctgttagttt
    19681 agtgaaaaca atcagtaata ttctaggagg agtcttgcta gggattctct ccatccagtt
    19741 catttccttg ctgaacgctc ttaccttttt aatcgcattt ttaggtatcc tcttcataaa
    19801 aactgacctc ttgaaagtag aaaaaacgat taactatcaa gaaggactct ctgtaaaatc
    19861 cttttgccag catttgctcc aatcatcgaa attgatatgg aatatgaata aggtgctctt
    19921 ggttttgttt attatctcta ctagtcaagc agtgataaat gttacagttc ctgtctctac
    19981 tctatttttg aggaatcaac cctttttgaa tttacaaaca ggtcaatctc ttgccttgct
    20041 atccactttt gaactgtcag ccctgattgt cggaagcctt gtaagtggct atctgcaaca
    20101 taccatttcc ataaaaacag ctctctatgc ctcacttgtc atccagttgc ttcttctagt
    20161 aggatttgcc acagttcgtt ttgactggat tctcatcttt agtaccttgg atgccttttt
    20221 cgcaggtgtt ctctctccta gattgcaaga actcgtcttt aaacaaatac ctgaggagtc
    20281 aatgggagca gttcaatcct ctatcggtgc cattacggtt gttttaccta gcttatttac
    20341 aatagctttg gtaaccattg ctactagctt tggaactctg gcagttagct ttgttttatt
    20401 gctatttctt ctagttgcct ttgttatgct tctgaatatt cgtgaaagta tttagtggag
    20461 aatttctata atatatgttt gagactggat tgccagtctt tttatgatgg tcaaaatcaa
    20521 cagacttttt tctcttttct gaatgtgtta taatagactg aatctaaaat a
//
LOCUS       DAVBDG010000034        20191 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBDG010000034 DAVBDG010000000
VERSION     DAVBDG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20191
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.034"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..617)
                     /locus_tag="VQ526_001268"
     CDS             complement(<1..617)
                     /locus_tag="VQ526_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2554985.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLEN"
     gene            complement(726..1244)
                     /locus_tag="VQ526_001269"
     CDS             complement(726..1244)
                     /locus_tag="VQ526_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2554986.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VQ526_001270"
     CDS             complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VQ526_001270"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2554987.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESSHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VQ526_001271"
     CDS             complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VQ526_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2554988.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2700..3590)
                     /locus_tag="VQ526_001272"
     CDS             complement(2700..3590)
                     /locus_tag="VQ526_001272"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2554989.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3587..4225)
                     /locus_tag="VQ526_001273"
     CDS             complement(3587..4225)
                     /locus_tag="VQ526_001273"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2554990.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4445..5242)
                     /gene="proC"
                     /locus_tag="VQ526_001274"
     CDS             complement(4445..5242)
                     /gene="proC"
                     /locus_tag="VQ526_001274"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2554991.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(5246..6508)
                     /locus_tag="VQ526_001275"
     CDS             complement(5246..6508)
                     /locus_tag="VQ526_001275"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2554992.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVS
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6518..7648)
                     /locus_tag="VQ526_001276"
     CDS             complement(6518..7648)
                     /locus_tag="VQ526_001276"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2554993.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7728..9611)
                     /gene="cbpE"
                     /locus_tag="VQ526_001277"
                     /gene_synonym="pce"
     CDS             complement(7728..9611)
                     /gene="cbpE"
                     /locus_tag="VQ526_001277"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2554994.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAENWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(9614..10501)
                     /locus_tag="VQ526_001278"
     CDS             complement(9614..10501)
                     /locus_tag="VQ526_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2554995.1"
                     /translation="MEIKIETGGLRLDKALSDLTELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVIAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIDHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10491..10952)
                     /locus_tag="VQ526_001279"
     CDS             complement(10491..10952)
                     /locus_tag="VQ526_001279"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2554996.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRASQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10949..11857)
                     /locus_tag="VQ526_001280"
     CDS             complement(10949..11857)
                     /locus_tag="VQ526_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2554997.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11850..12047
                     /locus_tag="VQ526_001281"
     CDS             11850..12047
                     /locus_tag="VQ526_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2554998.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(12099..12770)
                     /locus_tag="VQ526_001282"
     CDS             complement(12099..12770)
                     /locus_tag="VQ526_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2554999.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12824..13045)
                     /locus_tag="VQ526_001283"
     CDS             complement(12824..13045)
                     /locus_tag="VQ526_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2555000.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13317..14126
                     /locus_tag="VQ526_001284"
     CDS             13317..14126
                     /locus_tag="VQ526_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2555001.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKLRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14307..15182)
                     /gene="aguB"
                     /locus_tag="VQ526_001285"
     CDS             complement(14307..15182)
                     /gene="aguB"
                     /locus_tag="VQ526_001285"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2555002.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15192..16277)
                     /gene="aguA"
                     /locus_tag="VQ526_001286"
     CDS             complement(15192..16277)
                     /gene="aguA"
                     /locus_tag="VQ526_001286"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2555003.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALGRPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16274..17401)
                     /gene="nspC"
                     /locus_tag="VQ526_001287"
     CDS             complement(16274..17401)
                     /gene="nspC"
                     /locus_tag="VQ526_001287"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2555004.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEIMDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGVDDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPSLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17401..18660)
                     /locus_tag="VQ526_001288"
     CDS             complement(17401..18660)
                     /locus_tag="VQ526_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2555005.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(18657..19517)
                     /gene="speE"
                     /locus_tag="VQ526_001289"
     CDS             complement(18657..19517)
                     /gene="speE"
                     /locus_tag="VQ526_001289"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2555006.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            19773..>20191
                     /locus_tag="VQ526_001290"
     CDS             19773..>20191
                     /locus_tag="VQ526_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET2555007.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTCNPLLIKEFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRL"
BASE COUNT         5841 a         4650 c         3608 g         6092 t
ORIGIN      
        1 taattctcaa gctgtgagtt cattatgatt tgtaaatccg atatatcttc agcagctaaa
       61 aacacatttt gaaatgttcc aagtccgttt tcaccaaaac tatattctct cttactatac
      121 ttactcttat tattctctat atagttagag tcaaggttct taacctcttg aagttcattt
      181 tctttacttc tgaggttaaa atcttttact tctgaagtta agttttttga cttctgtaag
      241 tcattttctt ttacctgaaa tatactcata aagtctttaa cataaatgat gttcggctta
      301 ccaagtccaa gccttactct ttcgatcagt cctattcctt tttttacatc aagctcatct
      361 aatgttttta tggcagttgg ctttgagata tttcttcttt tcataatttc ttcgacagta
      421 aaatagataa atactctgcc ttccttgtct atccagttat tcttaaatga cattcctgtc
      481 tgtttaagaa gtatggaata aaggataatt gcttctgctg ataagccccg aaattcttct
      541 ctatcaacta atatctccgg tacttttaag aagttaaatc tttccgcttc tctgttataa
      601 aaatattcaa agtccatgca agatcacctc ccttcttaaa aatttgcaaa gaaaaagacg
      661 atagtttttc ttctatcgcc tttggtaacc atttttatga aattttttaa agttaaaaat
      721 ataatttata atactatctt cttccatttt ctgattttag ttctgaaagt tccaaaaggt
      781 gcaaccgtat ttacatgaat aaacttatac acttcccaag tcgctgtttt agtcgcttca
      841 tcagcccact ttctcatatg cggttcaaat aattcttcct cactcaacga atcaatcatt
      901 gcagagatag aattaatatt ttcatttaat tttgctttca actcttgcag agataaatga
      961 gcgtaggtat ctgtgaacca ctgatataat tcaccaagtt gattccattt aaatttatcc
     1021 gatggtgttt ttacttgaag cccctttctt tcatcttctt cccatttaag aaccaagttg
     1081 gtccaaccaa cctgataagc aaggttttct gctggagttc tgtcaacttc atcagctctc
     1141 ttatctttta aattttctgg aatattatca aattctaaaa tatatttctc aaatgttttc
     1201 tctatctcag cttttagttc ttctttattt tcgtatgttc gcaaaaaatc accttctttc
     1261 tgatttatca atcctttgat ttaaaatgac cttatttttc tatttatacc ttctaagaac
     1321 gctataactg ctgctccaac catcggtatt ccgtatgggc ttaataaaaa acttaatacc
     1381 aaagcttcta taccaagtga tacatcttta tattaccatc actaattaag ttaaacagaa
     1441 acagacagaa ttattacatt ctgtctccct ttattgtttt tcttcccaat cgtggtaggc
     1501 agcgtagagt tgactcttat tccactggta aatcttagct acttccttaa tagcttgatt
     1561 tttcttcatg ccttgctgga tacgggcttg gatttctaag aacaagtcct cctcgtcttt
     1621 ttcctccaca tcctgactgg caccttcaac aataagaagg cattcgccct tgagtggcgt
     1681 ttcagcgatg ctttccagca attcagaaat ggtacctctt tggtattctt catagatttt
     1741 agtcaattcc ctgaccaaga ctaccgagcg gtcaccgtag acttctagca tattttccaa
     1801 tgtatctgcc acacgatggg acgattcata aaaaatctga gtttcaggat agtctttttt
     1861 cgagtcgaaa aattgctttt gctgacctga ttttctcggt aaaaagccgt aaaagatatg
     1921 tggctgcggc gctaaaccac tggcaatcaa ggcagaaatt cctgcagagg cacctggaac
     1981 agtcacaact gcaatttctt cctcaatagc tgccttaacc aaatcatgac cagggtctga
     2041 aatgctaggc aaaccggcat cagagacctg agcaatactt tgccctgctt tcaagaaacc
     2101 aatcaaatca ggaatttttt ccttggcatt gtgctcatga aaactgatct gcttggtgga
     2161 aatgtcaaaa tgcttgagca aaagacctgt attgcgcgta tcctcagcag caatccagtc
     2221 cacttctttc aaggtctgga tagcgcgaaa agtcatatca tctagattgc caatcggcgt
     2281 tgccactaga tacagcttgc catagggaga ctgcccctta aaactttttt gaatttgcat
     2341 gcctactccc tgtataacaa ctcatcacag aacatacatt cttcgtcctg ctctcgacgt
     2401 tgtccataaa aatcattaca tacgtgaaat ccatcacggt aaatacgacg gacactttcg
     2461 cgaacatgct tggccttgac gggagcgtct gcttccacct cacccaagcg ttctcgcaac
     2521 ttactatttt ccaagcgaag agctgtattt tcctctacca ggctcttgag atttttcttg
     2581 atggcttcca catcggccaa ggttaccaat aattgttggg aaaaatcatc cagcgcgtca
     2641 aataattctt ttttgtccat aaaacagctc tttcctttct ttatcattca tttttgagtt
     2701 tatatttctt tcaagaccag atattccatg gcattttgaa agctgacatt agcttgccac
     2761 atttttctag cttctagtaa atcttgtaga atcactctta ctcttacctg caagaggtcc
     2821 tgcccacaga gaacttcaag aatccgtaaa acctgatcct gtttttcctt atcatctgcc
     2881 aagttggcta atttggcaac ctgtagataa ctttcttttt tcttagctac taaccaagtc
     2941 agcaggcgtt cactttcatc gaccaaggtc caaaaacttg cctgattagc caacttttct
     3001 gcttcagctc gcgattgact aaacttagct aaaagagtcg cttttttctt aacaagtccc
     3061 atttgttcta agagtaagat aagtttttct tcttgctttt taaagtggaa gatctgggtc
     3121 cgacttcgga ttgtcggtaa catcttttcc tcatcgctag tcaagaagaa aatataaact
     3181 tcactctggg gttcttcgat gaccttgagc agagaattgg ctgcgttggg atgcatttta
     3241 tccgcttgct cgatgataaa gacctgttgc tggctttcaa tccctgcttg agaaaactgt
     3301 cccaccaatt ccctaatgcg ttctgtcttg atgacctgat tgactggctt aatcaaggtg
     3361 acatcgggaa attctccctg ttcaatcagc ttgcaacttc ggcatttctc acatggtaag
     3421 acgccaactt tatccgtaca aaagaggctc ttagctaaaa attgcgctat ttccaagctt
     3481 ccaaagaaac ctgaaaagag ataggcgtga ttgagctggt cttgttctaa gatacggaca
     3541 aaacggtcaa actgagctgg ttgccaagcc tttagttgat cttgtttcat ttggccaagc
     3601 ccattccgtc aaacaagaca gccttggtag tttccacaac ttgctccaaa gggagactag
     3661 catcaatctt gacaatgcga tttccctctt tatccagaag agaaaggtag ccttgacgaa
     3721 cttttttatg caagtccaac ccttccaaat ccaaacgatt aacctcgcgg tcactattag
     3781 cagcaatacg agccagccct tcttccacct cgatgtcaaa atagagtgtc aaatcgggtt
     3841 tgaggccatc tgtcgcaaac tgattgagcc agtcaatggc ttcaatatct aagccacgac
     3901 caaatccctg ataggcaaca gaactatcga taaaacgatc catgatgacc aacttgccag
     3961 cttcaagggc tggaagaact ttttccacca aatgctgtct gcgactggca atatagagaa
     4021 gtagctctgt tttagcatcc atctgagtat gacttggatc caaaatcact tcccgaatct
     4081 tctccccaat caagactccg ccaggttcac gggtcgtcag cacctttact cctttttcct
     4141 ctaaaattgg tagcagagcc tctaaaacac tggtcttgcc tgctccctct ggtccctcaa
     4201 gagagactaa aaatcctttt gacatgtcta actcatttct tttttactac ttctattcta
     4261 tcaaaaaaat aggggtttgt gacaatcttt ttgtcttctc atttcatact caatgaaaat
     4321 caaagagcaa actaggaagc tagccgcagg ctgtacttga gtacggcaag gtgaagctga
     4381 cgcagtttga atttgatttt cgaagagtat caaccaccat aaaagaggca gataaaacta
     4441 cctcttattt acctagttct tgtgttcgtt tataggcttg atgaactgca tccatgaccg
     4501 ttcctcgaaa agcatgcgct tctaggcttg ctacgccagc aatagtcgaa ccgcctggac
     4561 tacaaacttg atctttcaaa actccaggat gttgctggct ttctaggacc aattgcccag
     4621 ctcctaccac agtttgggct gccattttca aggctgtttc tcgtggtaat cccgtctgaa
     4681 cacctgcatc tgccaaggcc tcaataaaga gatagacaaa ggctggtcca caacctgcaa
     4741 gacctgtcgc tgcattgatt aagctttctc ctaattcaac caagagacca gccttggcta
     4801 aaagctgaca aaagagctca ctgtcctcag ccctgcaatt aggagacaag gcataactaa
     4861 tcactccttg cccgatagaa gcaggggtat taggcatcat acgaataatt cggtgttggc
     4921 ttgggataag acttgctagt ttttctaagg tcaatccagc tgccatcgaa atcaaaagaa
     4981 gactttctct tttttcaagg atggtctggt attgagaaag cagttcagaa aactgagcag
     5041 gcttaacacc tagaaaaatc acatctgctt cttcgaatat ttcttcattg ttagaagtct
     5101 gaccaccgaa gttagcgatg aaagcatcca ccttgacttg actacgattg gcaaggagaa
     5161 tctcatctga cggtctagcc tgcaagacag acttggctaa actagcaccc atattcccca
     5221 aaccgataaa tccaatcttc atcccttact cccttatctg tccatcacca gtgaccacat
     5281 acttgtagct agtcaattcc ttcaagccca tgggaccacg cgcgtgcaat ttctgagtag
     5341 aaatccccat ttcacaacca agaccaaatt gtcctccatc tgtgaaacga gttgaggcat
     5401 taacatacac cgctgcagag tccacttgat ctgtaaagta tgctgcagct tcagcatttt
     5461 ccgtcacaat agcatccgaa tgatgagtgc tgtgggattc aatgtgcgaa accgcttctt
     5521 ctaaactgct cacaacctta acagcaagga catagtctaa aaactcggtg tcaaagtctt
     5581 gggtctcagc tgcttgacct gaaacaaact ggcttgcttt gctatccaag cggaattgaa
     5641 ttggttccag tccagcttcc ttacgctctg caaccagcat ttgatccaag cgaggaagga
     5701 tgcttgctgc cttgttttca tgaaccagca gaacctccat ggcattacaa acagaaggac
     5761 gactggtttt agcattgttg atgatagaca gcgccttgtc ttcgtctgca tccttatcca
     5821 catagacatg gacaatccca gtccctgtct cgataacagg tacaatcgca ttctcaacca
     5881 ctgcattgat caagccagct cctccacgag gaatgagaag gtctagatag cccttggcct
     5941 tcatcatagc ataactactt tcacggctag tatcctccac cagttgaatc acatttggat
     6001 gaatagtagt cgtctccaag cccttcttca aggctgtgac aatggcatgg gttgtttgat
     6061 aggcatcctt accactacga agaacaaccg catttccact cttaagagtc aaagcagccg
     6121 catcagacgt cacatttgga cggctttcat agataatacc gatgacaccc atagctacac
     6181 gtttttttgt gataaccaaa ccattttcaa gctgacttgt ttctaaaact tcaccgattg
     6241 gatctggtaa ggcaaccact tcacgaattc ctcttgccat cgcttctata cgatctgcat
     6301 ccaaataaag acgatccaac atcacatctg agattttccc cttagccgct gccatatcga
     6361 gggcattagc cgctaaaatt tcctcagtag cagccactaa gtgatcagcc atggctagca
     6421 aggcttggtt tttcacttct tcactagctg tgttaatcga ttttttaaca gcctgtacct
     6481 gttcaaattg ttcttgtcta ctcaccatag tttacctcta aaattctgta aaaagtagtt
     6541 ggatttcagg agtaatggaa atccagtcgt cacggtaaat caagacaccc ttggcttttt
     6601 gagaacgcaa catatcctcc aaagcagatg ctccaaattg cacgcgtcct tttccaagtg
     6661 attttccact ttccttgtca aatactgtca cgatatcacc gtaagaaaag gctccttctg
     6721 cttcaacgat accagataag agaagactct ttccatgttg agagagagct tccgcagccc
     6781 ctttatcaac ccaaatagaa ccttgactct gagcatagaa ggcaagccat tgtttctggg
     6841 tacgaagccc cttctcttga gcaacaaagt aagaaccatc ctcggtctcc tctgccgcct
     6901 caatcatgga atctgatttc aaggatgagc aaatataaac aggaactcct gattccgtcg
     6961 cgatagttgc agccttgatt ttggttaaca tacccccagt tccgtttgac gaacctgctc
     7021 caccagccat atcaataatc tcacgattga tggtctcgat tctctccaag cgtttggctc
     7081 ttggatctga attaggattt ccagtataga gaccgtccac atctgtcaag aaaactaaaa
     7141 ggtctgcttg gaccatcgcc gctacttgag cacttagagt gtcattgtcc ccaaccttga
     7201 gctcatcaat aacgacacta tcattctcat tgatgatagg aattgcccca cggttgagca
     7261 aaaccgacaa agcctgatgg gcatttttat aacgacgctt atccacaaag tcatcttggg
     7321 tcagcaagat ttgtgcagaa acgatttgac gcaagagaag attggtcgta tattcttcca
     7381 acaaaagtcc ctgccctacc gctgctgaag cctgtttatc agcaatttta gtcggacgct
     7441 ttttaaatcc taaggctcca aaaccagccg caatggcacc tgaagacacc aaaatcaact
     7501 catgaccagc ctcgtgcagc atagccaact gctgggtaat atcctttacc ttactacgtg
     7561 ataaacttcc atcctcattt gtcagagaag aagtacccac cttaaagaca atccgtttgt
     7621 atttcatagt ctcactccga ttattcatat ttatccatta taacatgaat aacaggaaat
     7681 agaaaagagt tgataggaaa aagaaggccc taaaaccttc ttttctacta ctgttctgat
     7741 tccgatttgt ttacactcgt ttcagagctt tgtgaagttg aggatgtcaa atcttgacta
     7801 gttgaagaat ttgtaacact ttcatgttga ctcgtttctt tattttcaag atcctttttc
     7861 actacttctt tggattcttt tatttctgaa tgacttgtag ttttaattgt agcagatttc
     7921 tcgatcggaa catccactaa ccaggcacca cttgaatcca cagtatagcc ttctggtgtc
     7981 ttaccattca caagtaattg accattttct ttcaagaagt accatttatc cttgtcttta
     8041 atccaaccta tagctcgtga accacctgtc ttgtagaaat accaatcatt tgccttttta
     8101 agccagcctt gcttcatagc tcctgattct tctagatagt aatgatgtcc agcaacttct
     8161 atctcacctg tcttcatgat accagtagaa tccatatagt accaggcttc tttatctttt
     8221 acccagcccg ttttcatttc ccctgatgga gcaaagtaat accattgccc cttatattga
     8281 agccaacctg tcttcataga gccatcaaca tccaaataat accactgctc tttatcttga
     8341 atccaaccaa tttccatctg gttttctttg ttaaaataat accagattcc agcgattttc
     8401 ttccaatttt cagcagcaac accagagtct gtcaaataga accaacgatt gttccatttt
     8461 ttccattgat tctgtaataa gatacccgtt tggttaaagt aataccattc acccttaatt
     8521 tcattccaac cgacagcata ttctcctgta gaatcaggcg cttgatacca ccagtttcca
     8581 tatgaactct tatgccaacc agcttgaaaa cttggaatag gcttgtagga agttgaaatg
     8641 tttactaaac cgtcttgtcg aatatcaaaa actgttgcat catagtcttt gctggctgcg
     8701 ttgattctct caattcctcg ttctttgagc caattaacat actcactatc aacaccattt
     8761 ttccaaggta gactatccga agtttgaaca atcaaactcg gactcaaatt tttaatgaaa
     8821 tccttggtat ttgatttgtt ggtatcatgg tgatgattaa acttcatcaa atcaactttt
     8881 ccaatgagag gaccatactt gtcttctgct ccatgaacat tatctaagtc gcccccaagg
     8941 taaattttct tgccattgac ttttaccaca ctaatcaagg aattggaatt atcatcccaa
     9001 attttcttta attcacctga tgaatcggtt tcattttcat agttatagag ttgaatatcc
     9061 atgtccccaa actgaaaatg agcatctccc tgtgtgatgt tttgaataac tgaaacacct
     9121 ttttctgaag cagtctgtaa aaccttatca taaccgtaca gattatccca tagacgttca
     9181 gaattagtaa tacgattatc actatatttt ttaagataga ctcggtcaac tggataggta
     9241 gacagtaatt catcaacatt tccaatatgg tcactgtggg tatgggtcac caaaataaaa
     9301 tcaagttttt ggacacccaa ttccttcaaa cgacgaaaga cacggtctgt tagaacatgc
     9361 ttataagacg tttcaattcc ttctctccat ggatagcgag aatcacttcc atctgggaaa
     9421 tcataatctt ctcctgtatc caccatggca aaatgtccat tgctttcaag aataatcgca
     9481 tcactgccac cttcttgaac attgataaag tggattttat ttcctgaact ttcttgagcc
     9541 tgaacattcc cataccatga caaacctaaa aaagcgccta caagtgctaa actagttaat
     9601 ttctttttca ttcttacttt ctcaatctct ccagggtttc cttaaaaatc tctgggatat
     9661 ctgctttaaa ttccaaggtc ttacctgttc tcggatgagt aaaacctaaa gtcttggcat
     9721 gaagaaattg tccatgtcct ttcaaagtct tgcgaggacc atagacctca tcaccagcga
     9781 ctggatggtc gatataagcc atgtggacac ggatttgatg agtgcgccct gtctccagtt
     9841 gcaactctac taagctataa tcgccaaagc gttccaagac atgaaaacgc gtcactgcag
     9901 gcttcccttt agcaattaca gcctgtttct tacggtcttt ttcactccgg ccaatcggcg
     9961 cttcaattac accacgatca ttaggcagat ttccatgaac aatcgcccaa tatttgcgga
    10021 gagacttttt atccttgagt tcttgggcaa gtgctagatg cgcatcatcg tttttagcaa
    10081 tcatgagaag acctgacgta tccttatcaa tacggtgaac aattcccgga cgcagaaccc
    10141 cattgatacc agacaagtcc ttaatatgat acatgagggc attgactaga gttccactag
    10201 tatgaccagc actcggatgc acaaccattc cctgaggttt gttaacgaca gccacatcct
    10261 catcttggta gactatttct agcggaagat cctcagccac atactctaat acctctggtt
    10321 ctggcacatg gtaagtgacg acatcaccct cttggactgt gtatttagct ttcttgactt
    10381 gaccattgac caagacctgg cctgatttaa tttgttcatt cgcaagactg cgtgataatt
    10441 ctgtcaaatc tgacaaagcc ttatccaaac gcagaccacc agtttcaatt ttaatttcca
    10501 tttatttcct cttttagcat tgcaatcaat aaaataatca ctccaaccgt cagatagcta
    10561 tctgccacat tgaaaattgc aaagttgata aagtcaaggt ggaacatatc cacaacaaag
    10621 ccctgactgg ccctgtcaat aaagtttcca agaccacccg cgattatcaa agtcaaaccc
    10681 aagaccatcc agaatgagtc ctccatgtgt ttatgtaaat accaaatggc acctatcacg
    10741 acaaccagag taatgacagc gaataacagc tgctgatctt gtaagataga aaaggctgca
    10801 cctcgatttt gcaggtaggt caagctaacg aaattgggaa tccaggagcg cacttcaccc
    10861 agtggaatct gctggacgat ataggatttg accaactgat ccagcccaat taaaagcagt
    10921 acaatgactg ccactattgc tctttttttc atgatttcct cttttgatca aaatattctt
    10981 gcatgacttc tacgaagaga gtcccagctt gactaagctc cacttcttca cgtttaacat
    11041 agaccatgcg gttatctagg ttatccttga gacgaataac tgtaatgcca ttaacactgt
    11101 cactatctaa aaatccagaa cctgtcgcat aggcgtccgt ccgctccaaa ataccattca
    11161 aggtggcacg gtctgtcaca ttaaacatct gtgagctagc gctggtatcg acaaagttct
    11221 ctgaataata aaggtactcg tctttctctt gagtgaaacg aaccgttggt aaatccgcta
    11281 aatcctccat gactaattcc tctttctggg ctaaaggatg accctcacag agataaatat
    11341 gggtatggaa aggaatcaat tcgatgacct ccagacctaa cttttcaacc cgttgcataa
    11401 tccccttttt attttgattg ttgaggtaga taatcccaat ctcactatgc ccttgcgcca
    11461 cttcatctaa tatttgaaca gtagttgatt caaaaatacg gaagttctta tagtcaggat
    11521 agcgctctga aaaggccgta atagttggtg gtaagaagtc atagtgctgg ctagcaacgg
    11581 aaaattcatc tttttcttct tcaggattgg catactgatt ttgaaaaata tcaaatcctt
    11641 taaccaattc ttgcgctttt tcataaaatt ccatcccacg acgggtcaag aaagtccctg
    11701 agctggtccg acggaaaatc ttaaagccca actctttttc caaatcacga acagaaatag
    11761 acagactcgg ctgactaaca tacatctttt cagcagcttc acgaaaagta ccactattgg
    11821 caatagccac aacatagcgt aattgttgaa tgttcatctt ctacccccaa cttctttatc
    11881 ttttcattat accatatttt agaagttttc caaaaaggaa aaaagaacat cctattcttc
    11941 ttaactatct tcactatctg cctttctcac gccaatctta ttttcaaaat caattcaaaa
    12001 aaataagtgg ctacacacca cttcagtatc ggaaagaaaa acgttgacta tttgtgaaaa
    12061 aagaaaatgc cggaaaattc cgacattttt ttagtaagct aacttcctga aaataggagt
    12121 aaccacacat cccagaaggc agtgatattg atgagaatat gaactagtat tggatagtag
    12181 agatttttag tcatgcgata caatagagct aaaataagac ctccactagc ataaatgaaa
    12241 aaagcaagag gagttaaagc aaaattgata tgaatataac cgaaaataat agcagaaagc
    12301 aaaacatctc cgtaccaagg tgagtttttg aaaaaggttg tcataagcac acctcgataa
    12361 atcaattcct cagcaatagg ggcgatgaag caaacgatga gcaagaaata agggaactcc
    12421 tgtctcccca tcatttctac cgtttcattc aaagaaatct gatttgaaga cagggatatg
    12481 aaatacgaaa agaggaagtc agacatatat gaaatgatgt agcccagtaa aaggtagatg
    12541 aagtacctca gctgccactt gaaatgaaaa attttctttc ctgcaaaaac cagtagatag
    12601 atgaccgcta agaaaagaac tccgctctcc atcaaaagca gaatctgaaa aaattcacga
    12661 cttgctggta gatagggctt tgcgaggtga ttgaaaactc tccaaaacca agttgatttg
    12721 taaaaaatta aggacaatgc tagtaaaatt tgaatagccc gttttttcat attaaattct
    12781 ctgctttctc cacttgtttc ttcatattac taataaattt tacttaaaat ctcgcagcac
    12841 ttcctttgca aagatgattg cctcctccaa tatatcctaa atcataggtg cctctgggac
    12901 aaaatccatg aaccggcaga gttaatactg gttctatttc ccaatcgtac ctaccattag
    12961 gatttttacc atatggatct gctccacctt gtatcagctc taattcttca tttgttagat
    13021 ttatattttt tgtcattaaa atcatgatat atttcctcca ttattcttca gattagttga
    13081 gtaatctgat actcatacct acttacaaaa aaactattat attaagttgg ttttttaatt
    13141 tgtctagttg caacattgac aaacatagta tagcatatct ttgccaaata tcctcttcaa
    13201 atcatgaatt gtcatcaaaa catcttaaac tataaaatca attagtctca agctttctat
    13261 caatttcttc tccaaaatat gctataataa tagcaaaaga taaagaagga agacctatga
    13321 ttaaactact agccttggat atggatggaa ccctcctcaa tgaagccaag gaaatcccac
    13381 aggctcacat cgctgctatt caccaagcca ttgaaaaagg tgtcaaactg gttctctgta
    13441 cgggtcgccc ccttttcggt gtcctcccct actacaaaaa actgggactc gacctccaga
    13501 atgagtatgt gattgttaac aacggttgtt caactcacca gactagcgac tggggcttgg
    13561 ttgactggca agaacttagt ccagctgaca tcgaatacct ctatgacctt gctgaaaaga
    13621 gtgatgttca gttgacactt tttgacgagt cacattattt tgttctcggt ggcaaaccca
    13681 atcaagttat tgaaaatgat gctaaactag tattttcaga cctgactgaa atttctcttg
    13741 aagaagcgac tagtggaaag ttacggatgt tccaaggtat gtttttagga acaaaagaac
    13801 aaacagacga ttttgagcag cgttttgctg aagagctttg ccaacgattc agtggagttc
    13861 gttcgcagcc tgtcatttat gaagcaatgc cgcttggaac gacaaaggct actgctcttt
    13921 cacgactagc tgagattttg aagattgatt cctcagagat tatggccatg ggcgatgcta
    13981 ataacgatat cgaaatgctc cagtttgcag ggcttgggat tgcaatggga aatgccagcg
    14041 attatgtcaa atctcttgcg gatgccgtta cctcaagcaa cgaagaagac ggcgttgcgc
    14101 gtgctattga gaaatatatt ctataaaaaa gaaaagcaaa tagacagaag ttactgctat
    14161 ttgctttttt gctaatattt taaaatacgg actaatcaat agacaagaat agcgaatcat
    14221 aaaaatcgat ttactagatg ccatagtatc ttatagttgc taataagaag ttagtctagc
    14281 agaatgaatc tctcatttct cccacactaa tctgtaattt gtctatacat ttctggtctt
    14341 ctatctcgaa acaagcccca gtttaggcgt tcacttgctc ccttgtctag gtcataagta
    14401 gctaacagaa cagcttcttc ttgtctttca gctcgttcta gaatagctcc tgtttcatcc
    14461 gtcataaagg aggaaccgta gaagtcaaga ctggaactct gtccgccatt ttcctcacta
    14521 ggagtaacct cctctaaacc ataacgattg gctgcgatga ctggaacaat attcgctgct
    14581 gcgtgccctt gcatagtacg ttgccagtga ccacaactat ctgtatccaa aatcggctct
    14641 gaaccgatgg ctgttggata aaagagcaat tctgccccat tcagagcaag acagcgggct
    14701 gtttcgggga accattgatc ccaacagata ccgataccaa tcttagcata gcgagtattc
    14761 cagaccttga aaccagtgtt accaggcgtg aaatagaatt tttcttgata ataatggtca
    14821 tctggtatgt gggtctttcg ataaacgccc agcacttccc catctgcatc aatgacggca
    14881 atagagttat acaagacatt accatctttt tcatagaaac tgattggtaa aacaacttgt
    14941 agttccttag caatcacctt aaaatgctga atggcagtat tttccgctac agattgggca
    15001 tactggtagt agtcatactg acgttcctga cagaaatagg gatgttcaaa caactcgggc
    15061 aagagaataa tttgggctcc ttgctcagca gcctgacgta ctaaacgctc tgcggtttgg
    15121 atatttgttg ccacatcctt agcgcattgc atctgaatgg ttgcaactct tacatttctc
    15181 atctttttct cctattctgg aatttgttgg gtgatacagt ggatattgcc accacctaag
    15241 agaatatctc tggctggtat tccgacaact ttacggtctg ggaaacactt gctgaggata
    15301 tctaaggcca cttggtcgtt tacatcctca aactgtggaa ccaagacagc cttgttggcg
    15361 atataaaagt ttacgtagga agctgctagt cgttcacctg cgtatcgctt ttcttctcct
    15421 tcttcataga tgtagcctgg caaatcttct tctgtcacaa cttgtcgaac tgcagggata
    15481 ggcaatttat gaatggtgaa gtgacaacct tttgcatctg tttcctgttc taagagttcg
    15541 agatctgctt ttgacatggc atactgggga tcattttcgt catctgtcca agccaaaaca
    15601 agctcagcag gaccaacaaa ggcagcaaca ttatcgacgt gttcattggt ttcatcctga
    15661 taaataccat aaggaagcca aataactttt tcagcaccaa gactttctaa taatgtgttt
    15721 tcaatctcct ctttagtcag gttaggattg cgaccaggac taagcaagca actttcagtt
    15781 acgagaatag ttccttgacc atcgctatgg attgcgcctc cttccagtac aaaaggttta
    15841 gcatcataga caggccttcc caaggcctca gcaaaacgac tggctacttg gtcatcctct
    15901 tcataatctt gataaagacc atcataggtg cctccccaag cattgaaggc ccaatccacg
    15961 gctaatttct tacctttatc attgacgaga atggttgggc cagtatcacg cgcccaggca
    16021 tcattggtgg gaatgtctaa ataaacaacc ttgtctccaa gataggattg ggcttcagat
    16081 agataggcct gctccaccaa aagatagact ctttcccctt ctgctatggt ctcgatgatc
    16141 tgagtaaatg ctcttttagc agcctttcct tgaaaaggcc atgatcctgg tcgagtcggc
    16201 catatcatga gggtaccatg atggggttcg tactctgctg gcatgtgata gcctaatttt
    16261 tttggactgt ccatcatgat aatctccctt taaagtcttg atagccaaaa gctttgagta
    16321 agctacagtc tccctgttcg tccatgagat agagacttgg caatccaata ccattaaagg
    16381 tattattttt gacaaaagaa taaatggcca tgtcttgaaa ataaagtctg tctccgattt
    16441 ggactggatt ttcaaaacta taatcaccaa tcacatcgcc cgtcagacag gtattagaag
    16501 aaagtctgta ggtatgggct ttttcctgtg cctcaaagcc atttctcaaa gatggacgat
    16561 agggcatctc aagtacatca ggcatatggc aggtcgcaga ggcgtctaaa accaagattt
    16621 ccataccgtt ttctacaata tctaatacct cagttgctaa ataacccgca ttaagcgcaa
    16681 tggcttcacc aggctcgata tagatttcaa gattgtaagt ttttcggata cgcttgattt
    16741 ctgaaatcag caaatccaca tcgtaacctt ctcttgtaat atgatgacca ccacccatat
    16801 tgagccattt tacctcatgt aagtagggac caaactgttc ttctactgct ttcaaagttg
    16861 tttgtaaatc atctactccc tgctcgcaaa gggtatgaaa atgaagtccg tcaaccaaat
    16921 ctagcaaatc actcggaatc ttgtctatag taactccaaa gcgagaacct ggtgcacaag
    16981 ggtcatagag cgcgtgatct ccttgagttg aacactgagg gttgaggcgc aaaccaacac
    17041 tgacaccagc ctctcgacaa cgcggaccgt gtttacgcaa ctgtctctct gagttaaaga
    17101 cgatatggtc cattatctct agcaattcct ccaagtctgc atccttgaaa gcaggcgcaa
    17161 atacatggac ttcaccagga aattcttccc ttgccaattt ggcctcatag agtccactag
    17221 ctgtcgtacc tgatagatac tggctaatca agggataagt tttgtagagg gaatatgcct
    17281 tctgggcaag caagaccttg caaccggcct cttcttgtac atattgtaga atgcggcaat
    17341 tagcttctaa cttggccaag tcaataacat aggctggtgt tggtacttgt tctaacttca
    17401 ttagtccacc atttgtggat tttcaaccac aacccatggc aaaccatact cattcaaagc
    17461 ttccatgaat ggatctggat ctaactcctc aaggttatac actccagctt gtttccaagt
    17521 tccgttcatg actaattttg tcccaatcat ggctggaacg cctgtcgtgt atgaaattgc
    17581 ttgtgacccg acttctgcat agcactcttg atggtcgcaa acattgtaga tgtagatagt
    17641 cttttcaacg ccatctttga cacctgtaaa gatacatcca atattggttt ttccgactgt
    17701 acgtggccca agactggcag gatctggaag caaggctttc aaaaattgaa ttggaacaat
    17761 ttcttggccg ttaaagttaa tggtatccgt acgaaggagt ccaacatttt caagacattt
    17821 catgtgcgtc aagtaagatt gaccaaaagt cataaagaaa cgaatgcgtt tgacacctgg
    17881 aatgttcttg gccaatgatt cgatttcttc atggtgaagg agatacatat ctttttgtcc
    17941 aacttgaggg aaatcatact cacgcttgat agacatagct tcgacttcga cccatttccc
    18001 atcttcccag taagaacctg gagcagaaac ctcacggaga ttaatttctg gattaaagtt
    18061 ggttgcaaat ggataaccgt ggtcaccgcc attacagtct aaaatgtcga tataatggat
    18121 ttcatcaaaa tagtgtttga gggcataagc tgaaaagaca ctagttacac ctgggtcaaa
    18181 accagaacca agaagagcag tcaagcctgc ttctttgaat ttctcttgat aagcccactg
    18241 ccatgagtag tcaaagtagg ctgtaaaacc aagttcctta caacgttttt cgtagatagc
    18301 acgccactca gggtcttctg tgtcttctgc ttcgtagttg gctgtatcga tatagtgaac
    18361 acctgttgcc aaacaagcat ccataatggt taaatcttga taaggcagag ctacattcaa
    18421 aacggcttct ggtttgtagc tttcaatcag ggcaatcact tcttcaacct tgtcagcatc
    18481 aagtgctgca gtttcaattt tagtacttgt tttgccttct agcttcgctt tcaagtcatc
    18541 gcattttgac ttggtacggc tagcaatcat aatctctgta aatgtttcgc tatcttgaca
    18601 aatctttgaa atagcaactt gggcaacgcc cccacaacca ataactagta aacgactcat
    18661 ttttttcctt cctcttcttc taaaatgtcc tcaacatact tgggcaacat aaaggatccc
    18721 acgtgtaagt ttgcagtgta gtattctgtg aaaagctggc gttttttcca accttccttg
    18781 tcaaaatctt tgacagggtg gtattttttc gatgcaaatc caaacaacca atagccagct
    18841 gggctagttg gaatatgggc ctgataaacc cgactgattg gaaaggattg attgaccttg
    18901 cggtgcatgc ttcggcaggc cgactcatcc tcgtcaaaga agggactccc atgctggtaa
    18961 atcatgatgc cgtcttcctt cagagctcga taactattgc cgtagaattc cttggtaaag
    19021 agtccttccg tatggccaaa tggatctgtc gcatcgttga tgataatatc gtaatcatct
    19081 tcgcagtttc gcaaaaagcg tagcccattt tggtagtaaa tggtaacacg aggatcatct
    19141 agccctgcag caaagtctgg gaaatactca cgacagacct cgaccaacat ctcatccggt
    19201 tccacgatat cgatttgttc cagttcagga tagagtgtta atacttgggc aacaccgccg
    19261 tcaccacccc caataaccaa tactttcttt ggatttgggt ggacagccat gggaacgtga
    19321 acggtcattt cattgtagac gaaatcatcc gcatctgaga acaagacatg gccatttaaa
    19381 atcagtattt tcccaaaagc tggcgtatcc aagacttcga tatcctgcca ttcacttttt
    19441 ccagcgtaaa gttgcttggc tgttctcaga gacaatttga catctggagt atgaacttca
    19501 gaaaaccata aatccatcta tttctcctta tattatattt agcgcagtta gctactgcca
    19561 aagcccaagt ggtatacttg gaataagcca ctgtggatta gtttattccc tccaatcgct
    19621 tgacgatttc cgaaaggctc taaccacagt tttttgtttc aattggtaat gagtttgata
    19681 acgcatagac gttttatctc gtctaaggta acaagacaaa aaattcgtgg ataaccaagt
    19741 agacttgaaa tactacatct ggaggtactc ccatgcttta tgtgggcatt gatgtcgcta
    19801 aaaataaaca cgatgttaca gccttgaatg ttccaggaaa aactgttctt aaaccactca
    19861 ctttttcaaa taataaagct ggttttgaac tcttagatct gtctcttcga caactcaacc
    19921 aagactgtct catcgctctt gaaaataccg gacattatgc ctttaatcta ctgaacttcc
    19981 tgcatgaaca aggatataaa gtttacacct gcaatccttt gctcatcaag gaatttgcca
    20041 aatcattatc acttcgcaag actaaaacgg ataagaaaga cgctcatggc atcgctctta
    20101 aacttctttc tgaccccaat cgtgaacaat ttcaacacga taatcggcaa gtagaactaa
    20161 aaatactggc tagacatatt catcgtctca a
//
LOCUS       DAVBDG010000035        19947 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBDG010000035 DAVBDG010000000
VERSION     DAVBDG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.035"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(553..1422)
                     /locus_tag="VQ526_001291"
     CDS             complement(553..1422)
                     /locus_tag="VQ526_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2555008.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(1645..2325)
                     /locus_tag="VQ526_001292"
     CDS             complement(1645..2325)
                     /locus_tag="VQ526_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2555009.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDK
                     PTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGF
                     FKKKEDEK"
     gene            complement(2283..3389)
                     /locus_tag="VQ526_001293"
     CDS             complement(2283..3389)
                     /locus_tag="VQ526_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2555010.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWVRSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELILENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3500..3754)
                     /locus_tag="VQ526_001294"
     CDS             complement(3500..3754)
                     /locus_tag="VQ526_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555011.1"
                     /translation="MLNVDQDFMSISKPNKSGSDWKKTFTVRTTNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(4112..4645)
                     /locus_tag="VQ526_001295"
     CDS             complement(4112..4645)
                     /locus_tag="VQ526_001295"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2555012.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     RAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(4632..4847)
                     /locus_tag="VQ526_001296"
     CDS             complement(4632..4847)
                     /locus_tag="VQ526_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2555013.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCALPSVWAALKYIKVTLKKDDEL"
     gene            complement(4814..4957)
                     /locus_tag="VQ526_001297"
     CDS             complement(4814..4957)
                     /locus_tag="VQ526_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HET2555014.1"
                     /translation="MAYSTDFKQRALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            5191..6909
                     /locus_tag="VQ526_001298"
     CDS             5191..6909
                     /locus_tag="VQ526_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2555015.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            7020..7367
                     /locus_tag="VQ526_001299"
     CDS             7020..7367
                     /locus_tag="VQ526_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2555016.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     SGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7645..8481)
                     /locus_tag="VQ526_001300"
     CDS             complement(7645..8481)
                     /locus_tag="VQ526_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2555017.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8494..9123)
                     /locus_tag="VQ526_001301"
     CDS             complement(8494..9123)
                     /locus_tag="VQ526_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2555018.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(9133..9774)
                     /locus_tag="VQ526_001302"
     CDS             complement(9133..9774)
                     /locus_tag="VQ526_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2555019.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(9920..11149)
                     /locus_tag="VQ526_001303"
     CDS             complement(9920..11149)
                     /locus_tag="VQ526_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2555020.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(11139..12305)
                     /locus_tag="VQ526_001304"
     CDS             complement(11139..12305)
                     /locus_tag="VQ526_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2555021.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12449..13462
                     /locus_tag="VQ526_001305"
     CDS             12449..13462
                     /locus_tag="VQ526_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2555022.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(13507..13926)
                     /locus_tag="VQ526_001306"
     CDS             complement(13507..13926)
                     /locus_tag="VQ526_001306"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2555023.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(13937..15343)
                     /gene="atpD"
                     /locus_tag="VQ526_001307"
     CDS             complement(13937..15343)
                     /gene="atpD"
                     /locus_tag="VQ526_001307"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2555024.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(15429..16307)
                     /locus_tag="VQ526_001308"
     CDS             complement(15429..16307)
                     /locus_tag="VQ526_001308"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2555025.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(16323..17828)
                     /locus_tag="VQ526_001309"
     CDS             complement(16323..17828)
                     /locus_tag="VQ526_001309"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2555026.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(17843..18379)
                     /locus_tag="VQ526_001310"
     CDS             complement(17843..18379)
                     /locus_tag="VQ526_001310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2555027.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(18379..18873)
                     /gene="atpF"
                     /locus_tag="VQ526_001311"
     CDS             complement(18379..18873)
                     /gene="atpF"
                     /locus_tag="VQ526_001311"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2555028.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(18887..19603)
                     /gene="atpB"
                     /locus_tag="VQ526_001312"
     CDS             complement(18887..19603)
                     /gene="atpB"
                     /locus_tag="VQ526_001312"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2555029.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(19638..19838)
                     /locus_tag="VQ526_001313"
     CDS             complement(19638..19838)
                     /locus_tag="VQ526_001313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2555030.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         5953 a         4332 c         3616 g         6046 t
ORIGIN      
        1 ctgtgttttg aacaacctgc ggctagcttc ttagtttgcc ctttgatttt cattgagtat
       61 aaaattgcag ataaaacgat aacaatcata ggactcagca gagtcaatgt aggtcgaaga
      121 atagcccccc taatcgctta ttttatagca aaatgaaata gaaactgaac attttgttga
      181 ggaaggtaaa tcaattcctc acaatgtttg agaaagaagg gaggactatt ccaacttcaa
      241 cccactataa agcagactta caagataggg aacccatctc cgataatata tagtagaatg
      301 aaaactgcac aatttgattg gagaattcaa accaatttct aacaatgttt tagaaacaag
      361 ggtctattat ttcaactgca acctactata gcccattgaa gctggaatag cccactgaga
      421 cttctaaacc actgctggag aggagtttgt ttagagtttc tttcatttcc ctaaaaaatc
      481 aaaaagagag aaccagtctg atcctctctt acttatagat ggtgctcact caaatgttaa
      541 aggagctatt tcttagagat aaaggaattt gaatacagct actgccgcaa ttgctgctgc
      601 gataggtgct actactggta cccaagaata ccaccatttt gaatcgcctt tatgctcacc
      661 aagaactgat tttggaagga aagcatgaag gagacgtggt cccaagtcac gggctgggtt
      721 caaggcaggt cctgtaggtc ctccaagtga tgttaccaaa gccataacga ggaatccaag
      781 tgccaagtga gccacagaaa gtcctgaagc agtgtgtgga gccacctgtg ctttaatagc
      841 caagtcagaa aaatcaactg tttgtcctgc ttctattgcc ttttgtttca tgaattgaag
      901 cacttcagca ccaaagaagt ttttagtcaa accaagagct gcaaagaaca aaacaaatga
      961 accaacaaac tcattaatca aaccattgac agttgctgca tagcgacttt cttttgtacc
     1021 atggtcaata cttgaaatag ttgagaaagt tcccaagatg ttatttggtt tttcagtttt
     1081 caagtagaat ggacggtatg ttgccacaac taaggcttgg ccaaagatag cccccaagac
     1141 ttgcgcgata atgtaaggta ccacttgtgc ccaagggaaa agaccactaa ctgcaagccc
     1201 tagagtgaaa gcagggttga tgtgattccc agatacgtta ccaaacatca aggctgggat
     1261 cataaccccc ataccataac caacagcgat gacgatccag ccactttggt gacctttcgt
     1321 acctttaagt tcaacgttgg caactgcacc attcccaaga atgatcaaaa tggcagttcc
     1381 caaaaattca gtggcatact tcagtgccca tgtgaaatcc atttgctaga ttctccttaa
     1441 aattttttag atattcctta ttttatcaat ttttacccta tttagcaact gatttctgga
     1501 aatattttta aaaaaatagg attcatagtt tcctagttca acgtacttgt aagcgttttt
     1561 attcttcacg aaagacctca ttctcaaaac caggaaaaag cccacatctg tgatcttttc
     1621 cttgttccta gattctaaaa tctattattt ttcatcttct tttttcttga agaatcctaa
     1681 tcctagacca gctaacaagg tcatgatacc agcactagcc aaggtagcat tagcttctgt
     1741 acctgtgttt ggcaattcct ctctcttacc tgtctcataa gtcggaactt ctgggtctgg
     1801 attcactgga gtttcagttt ttggagtacc tggttctgga gttggtttat ctggtgttgg
     1861 agtctcttga tttggtagtt ctgggtttgg tgtcggaaca cttggatgct ctggatttgg
     1921 ttgtgggatt ggtttttctg gagtcggttc acctggagta cctggttcct gaggacttgg
     1981 aatttctggt ttttctggag ttggtttatc tggtgttgat aaacggtcat accttaccgt
     2041 tatttcttta tcactagagt ctgacgtaac ttcttgtgat tcaactgttg gaatatctgg
     2101 atctttgtac ttgtcaatct taccagatat aacctcgtcc cagtttcctg ttgtccattc
     2161 accgtaggtt acaactcccg tgaccttgtt ctcagttttt gtacggctta aggttacagg
     2221 ttgaacaaca tcttctttta cattttggtt cgtaacttta tcaacgtaat gaatgatacg
     2281 cgttatagtc ttcgtctcag tagaggttgc tgttttggga accactgttt cctcaacatt
     2341 ctcacggtag taatagtcaa ctgttgcacc gtcttctggt acgcatttgc aggagttgct
     2401 accaaggtgt atgttgtttt ccttgtgata actcggtctt ctttgtcctc agttgttgtt
     2461 ttcccttcaa tagtttttga ttctgtggta tactcagaac ctatcgctaa atcagctttt
     2521 ataacagact ctgccaactt ctcttggcta ccttctttat agtaattggc tgttactgta
     2581 gcagtggttg gcgcttcgct ttctctataa actaaggtca ctgttctacc ttcgcttaca
     2641 atattcccag ttaaacttgc agaatttgta tctgcttctt taaaagtata atattttccg
     2701 tcagtagtag tcatactact gagtttttta tctgtgacat tgtagctggt accaatcagt
     2761 tgttttttat tggtaatgta ggttccgtca ctttcttttt ctccaattcc agtatcattt
     2821 ttcaatgata gcaaacgccc ttgttcatca acatagcgaa ctttcacatt ttctaagatt
     2881 agttctgcca attctgaggt ttttttcttt ttcttgattt cttcggttat tttccctttc
     2941 tcttcttcgg gaatatttag ttttggaatg attttttcaa caacggttcg tgatggttcc
     3001 acagtatctt ggatgactga aaagtcagct agaattggga gattatactg aacacggtga
     3061 ctttgagtgt ttactcctac tctttcatta ttctctgaaa atacttgtac ggtataagaa
     3121 acaacatctt ttcctaatag aacatcccca gtagagaaat agccgccttt tcctagtttg
     3181 ctatctccag agcccacttc tttcctaatc ttatcagata gttttttacc agtcagtaca
     3241 ttcgttcgca caatcccttt gtctacccct acaaagtggg agaacttttt gaactcttca
     3301 gaaccagatc taacccaacc attattaagg gcatttgctt ttgtatttgt attctctctc
     3361 aaaagtttgg cgattagaat tatattcatc ggcacttaga gttgctgcta tatctgactc
     3421 ttgaatacca acttccttac taccatttct agcggcagta tatgtgtatt tatctgtttc
     3481 tacttctagc tgattatcct taacatgata ggtaattttc tcctggtata agagtcttca
     3541 gtagtttata tactctgtca tcatctttag ctttagaagc agctgagttt agccaagttg
     3601 gggtattagg agtatcttta tcaacctgtt tcaagacatt attcaagtca tttgctagcc
     3661 tattggttgt cctcactgtg aaagttttct tccagtctga accactttta ttaggcttag
     3721 atatgctcat gaaatcctgg tcaacattca acacaatatc gccttgtttt ttagcatctt
     3781 ccgttgcttc tgcttcaaat tgatcatgga gtttttgttg ctctggtgta tagttttttt
     3841 atttacacga tttaatgtac tattttttat tttagtatta agaactccat taatacgatt
     3901 tttaaattct gcttcaaaaa atcccttaat ttgttggtgc gcaagttcaa cattcaaacc
     3961 tttcttatac aagtcttcat tctgatacaa gtcttcattc tgaacgattt cggctacacg
     4021 gtgaatggtt tgattgtata ccgtcttctc ttaaccagca gtatccttag ttttaatgac
     4081 tactcgatag gttcctttag aaccgttata gtcatctagt tttaaaaaag cattccaaac
     4141 attcaaaaat agttggaact tcccttaata aatccgtcac tttctttttc aagatagccc
     4201 aagcttgctc aataggattc aaatccggtg aataaggtgg tagaggtaag aaaaagtgtt
     4261 tatcttggat gcagagttca tccaaaatgt tcttgggatg aaaactagca ttgcccatga
     4321 caataacatg aggtgtcttc aaagcaggta ggagttgcgt tttgaaccac tccacaaaga
     4381 aatcgcttgt catgcttttc ttgtaaatca tgggagctat aaactctccg tctacttgtc
     4441 ctgcaacaat tgaagttcgc tcaaaacgac gtccgctaat cttttcatag actttctccc
     4501 ctctaggagc ccgtgcataa ggacgataga ggtagcggtc gattcctgtt tcatcaatat
     4561 atacgattgg taaatctttt aggttatcca aaatatcaag aaactcagcg actttctctg
     4621 gctcttgttc cttaaagctc gtcatctttt tttaaagtga ccttaatata ctttaaagct
     4681 gcccatacgg agggcagagc acaatcaaaa tgtgccgcaa tttcccgtaa aaaagcatct
     4741 ggatgagcct ctacaaaggc tttcaattcc tctaaaggaa tctttcggtt tttgacgact
     4801 cgctttttcc tctctatgtg tccttgttca catctttctt ttcccacgtg aagagagttc
     4861 tgacgccaac accaaaaaac ttggctgcct cgacatggct gtgcccctct ttgatggaat
     4921 ctaatgctcg ctgtttaaaa tcagtactat atgccatagc tttattataa tatgcttgtt
     4981 taaaactaag caactatatc aatgttttgt tattagaaga cttacttttt ttactgaaat
     5041 cgaatttttc ctaacctcaa catcatataa ccccaaaatt caatctgacc tggtttgaag
     5101 agatgttcga agcagataag gttttactaa aactaaaaaa aatgatataa tcaaactgat
     5161 taaatgtttt aaaaataaag gagaatttga atgtcttacc aagaaaatta ccagaaatgg
     5221 gttgattttg tggagcttcc tgactacctt cgtcaagatt tggaaaatat ggacgaaaaa
     5281 actaaggaag atgccttcta tacaaatctt gaatttggta ctgcaggtat gcgtggcttg
     5341 gttggtgctg gtacaaaccg tatcaacatc tacgttgttc gccaagctac tgaaggattg
     5401 gctcgtttga ttgagtcaaa aggtggaaac gagaaagaac gcggtgtagc aattgcctac
     5461 gatagccgtc acttctcacc tgagtttgcc tttgaatctg cggcagttct tgctaaacac
     5521 ggcatcaaat cttacgtatt tgaaagcctt cgtccaactc cagaactatc atttgcagtt
     5581 cgtcatctca actgtttcgc aggtatcatg gtcacagcca gccacaaccc tgctccattt
     5641 aacggttaca aggtttacgg tgaagacggt ggacaaatgc ctccacacga tgcggacgct
     5701 ttgactactt atatccgtgc aatcgaaaac ccatttgcag ttgaagttgc tgatgtggaa
     5761 actgaaaaag cttctggctt gattgaagtt atcggcgaag ctgttgacgt agaatacctt
     5821 aaagaggtta aggacgtaaa catcaaccca gccttgattg aagaatttgg taaagacatg
     5881 aagattgtct acacaccact tcatggtact ggtgaaatgt tggctcgtcg tgctcttgcc
     5941 caagcaggat ttgactctgt tcaagttgtt gaagcgcaag caactgctga cccagacttc
     6001 tcaactgtaa catctccaaa cccagaaagc caagcagcct ttgcccttgc tgaagaactt
     6061 ggtcgtcaag ttggtgcaga tgttcttgtc gcaactgacc cagacgctga ccgtgttggt
     6121 gttgaagttc ttcaaaaaga tggtagctac ctcaaccttt caggtaacca aatcggtgct
     6181 atcatggcta aatacatctt ggaagctcac aaaaacgctg gaactcttcc tgaaaatgcc
     6241 gctctctgca aatctatcgt ttcaactgac ttggtaacga agattgctga aagctacggc
     6301 gcaactatgt tcaacgtctt gacaggtttc aaatttatcg ctgaaaaaat ccaagaattc
     6361 gaagaaaaac acaaccacac ttacatgatg ggatttgaag aaagcttcgg ttacttgatt
     6421 aaaccatttg tacgtgataa agatgccatc caagccgttc ttgtcgttgc tgaacttgct
     6481 gcctactacc gttctcgtgg tttgacactt gctgacggta tcgaagaaat ctataaagaa
     6541 tatggctact acgcagaaaa aacaatctct gttactcttt caggtgtcga tggtgctgaa
     6601 caaatcaaag cgattatggc taaattccgc aacaatgctc caacagaatg gaacgcaaca
     6661 gctatcactg tcgtagaaga cttcaaggca caaactgcta ctgttgctga cggtactgtt
     6721 acaaacttga caactcctcc aagtgatgtg ttgaaataca cacttgctga cggttcatgg
     6781 attgccgttc gcccttcagg tacagaacca aaaatcaagt tctacattgc agttgtaggg
     6841 gaaaccaacg aagaatcaca agctaagatt gctaacatcg aagcagaaat caatgcattt
     6901 gtaaaataaa gacctataaa agatgagacc aaccaacctc atcttttttc gtatcaaatc
     6961 taagcaattt tagaaacttt atggcatact agacttatca atgaaagcga ggtaatctca
     7021 tggaacaatt tttagataac atcaaagacc ttgaagtcac tacagttgtg cgtgcgcaag
     7081 aagctcttga taaaaaagaa actgcaacct tctttatcgg tcgcaaaact tgcccttact
     7141 gccgtaaatt ttcaggtaca ttgtcaggtg tcgtagctga aaccaaagct cacatttact
     7201 tcatcaatag tgaagaacca agccaactca atgatttgca agcattccgc tcacgctatg
     7261 gaatcccaac tgtaccaggt tttgttcata ttacagatgg acaaatcaac gtccgttgcg
     7321 actcttcaat gtcagcacaa gaaatcaaag atttcgcagg attgtaaaaa atcagctaac
     7381 aagtcttaaa atcacaaaaa cgcataatgt aaggtgcaaa aaccttgata ttatgcgttt
     7441 tattgttcct aaaacagtta catcagcatt tcattacaga acattttaat tttggaagtc
     7501 aaatcaattt ttaacaatgt tttagaaatc acagtgggct attccatatt caattatcca
     7561 gattcaatcg ctattatcgt tttcctagat tacaaaaaaa ctacgttttg ctaatcaaaa
     7621 cgcagtttca gccactattt tatcttactg tccttctttt acttctttgg ttgctacatc
     7681 ttctccaaac catttttgac tgatttcttg gaacttgccg tccttgtaaa gactagaaaa
     7741 agcttcattt atcttcttaa ccaagtttgt atcttcctta cgggctccaa ccgcaaaagc
     7801 ttctgtttct agtccaactg taaagacatt ataatcgttt aaaacacctt ctgcttctaa
     7861 ataataattt gcatagacac ggtcaatcaa tagaccatca attcgatcgt ttttcaaatc
     7921 aatcaaggct tcattaaagg tttggtattg attcgcttcc ttattagcga caatattctt
     7981 caaaatttct ggatttgctt caaagtccgc ataaccagat gaaccagctt gagctcctaa
     8041 tgtctttcca gtcatatcct ttgcagtcgt gataccagat gatttcttcg taaccaatac
     8101 ctgctcattc ttcatatatg agttactgaa agccaccttt tcacggcgtt cgtctgtagc
     8161 ggaatagcca ttccaaatca gatcaatcgt tccttttgtc aattcagctt ctttcaaatc
     8221 ccaatcaatc ggttgccaat ttaccgtgat tccgtatttt tcaaaaacag ctgtagctaa
     8281 atcaatatca aatcctgcat aagaaccatc tttctgagca aatcccattg gaacaaaagt
     8341 actatcaaat ccaatagtaa tagacttgtt agactggtac tttgaccaat tatctccact
     8401 agtttcgcta gaatttttcc cacaagctac taagaacaaa gcagtcatca gactgactaa
     8461 tacaagcatc cattttttca tccatttttc ctcctattta ggttctactt tcaataatac
     8521 atctgcgata ttttcagcaa actgcaaatc atgggtaacc acaatctggg tcatcccaag
     8581 ttccctattt tgcaagatta gcttctccac ttccaaacgt aattctggat ccagggcaga
     8641 agttggttca tcgtagccaa tgatttctgg gtcaatcatc atagcacgcg ccaaagccac
     8701 ccgctgcttt tgcccaccag atagtgagaa aggataggcc tctgcgtgtc ctcctagtcc
     8761 taactgttcc aagagtccac tcgccttctt ctcagcctct tcctgcttca ttcccatggt
     8821 cttcacaggc gataaagtca aattttccag aactgataga tgaggaaata gttgaaaatc
     8881 ttggaagaca aatcccagta ggttgcgctt ctgcaattca tctagctcta aaggttgtcc
     8941 attataaaag atttgccctg aatcaatggt ttcaagacct gcaagcatac gtaaaagagt
     9001 tgtcttacct ccaccagaag gtccaacgat agccaggatt tgcttttcag gaatacttag
     9061 actgaaatca gacaggattt gtttgtctcc aaagacttta ttgatatttc gtaattctaa
     9121 catagctgcc ccctatctat aataactgta ctttttctca acttttttgg aaataattgt
     9181 cacaatccca attaaaatca agtaaatggc tcctgccaag aacataggaa ccagactagc
     9241 atcgcggtta gcagctgttc gactagccaa gataaggtct gaaattccga gagcatagac
     9301 caaagaagta tccttgacca aactcataac ttcattaaag acactaggaa gaacgatctt
     9361 ggtcacttgg ggcaagataa tatagcgcac tctgtcaaaa gggctaaact tcaagacctt
     9421 ggcagcctca tactgtcctc ttggaatagt gtcaattccc ccacggaaaa tttctgcaaa
     9481 gtaagctgca tagttgagaa caaaggcaat aatagctgca ggaaggcgat ctaaacgaat
     9541 cccaatactt gggagcacat aatagataaa aatcagttgc aagagtaagg gggttcctcg
     9601 cataacccag atgtaaatgt taatcagata atggaggggc ttccaatgga cttgcaaggc
     9661 aaaggcaatc aaaacgccca agggaatcga aaatagcaag accagtgcaa agacctgtac
     9721 agtcgtgctc gcaccattca gtaaactcgg taatatctca aataaataag acatactgca
     9781 cctcctaaaa gtaattgttc ctattatagc atgaataaac aagattacca agtatttttg
     9841 taaaaagatt ctaccatata caaataaaaa ccgtagctat caaaagagat aaactacagt
     9901 tctatatgtt taaacattct tacaatctct caaacagttc ttgcatttgc acatcatctg
     9961 gaaccagttt taagtaagtg tgagcatgga cttttgcttc ttcaaaatgt cccaattcac
    10021 gcaagagata gatatagtgt tccagaaatt ctggattgtc cttcaaatct cctgtcaact
    10081 cttgataatg ctcataagca gtatccaaat cgtccatttc ttgataagaa cgagcaatca
    10141 tccacttggt caaaagattt tctggctcct cactctgcaa gtctagaata tcctcataac
    10201 gctcctgctc cagataaata gtggctaaac gaagcaagat ttcttctgta tcctcagcgt
    10261 cttcttttgc agtaaggaga taattttctg caccactagc atcatgcaat tcataagaaa
    10321 attgtgaagc agctagcaag aggcgagttt caaagggatt tttctctaat ccttgcttag
    10381 cgatacgcag ggcttcttga acttgatgtt ccttatgtaa agcctgactg tacccatact
    10441 catagccttc aaagtcagga gaaatggtat caagctgctt aaagtagagg gtggcttttt
    10501 gatattcttc ttgatcaaaa taaagactgg ccaactcaaa agctgttaag tcatcgtatt
    10561 ctaactccag ggctttttct aaaaactcag tagccgtttc aaatttccct aactgagcat
    10621 aggcaaagcc aattcgttga taggtggaaa tgcccgtttg ctcataaatc gagcgattat
    10681 ctaactgggc ataggcttga atagccgctt ggtaattttc caactcacta tccaactctg
    10741 ccaaacccaa tatcaagaga gaatcctctg agtaggtcaa ggcctccaat aatttctcac
    10801 gtgccacatc tgtcaaacct tccagctggt aaaggtctgc cttcagagcc aaagacgaga
    10861 cataccagtc actgtcagct tggatttcct caagataggt aaaggcttct tctatttgac
    10921 catcctcgct agcaattgca gctagattaa gatgaacctc tggaaaatcc tctacaattt
    10981 tcaggtaaat ttccttggcc tgaggataga aaccaatccc ttcaagataa gttgccaact
    11041 catacagaag atcacttgaa tcattttcta aagctttggc gaaataatgc tcagccttag
    11101 ttaaatcttg ctcctccaaa gcctgtaaca tctgttgact attgttcact cttgacttcc
    11161 tcaccctcta attcatatag accactgact accttatacc attcgaaaat ggctgaaatg
    11221 acaaccttag cagaggcata aaccggaata ccaagtaaaa ctccccagat accaaacata
    11281 gatcctgaag ttaacaaaac aaagagaaca ttaataggat ggatgtttaa ttgacttccc
    11341 aaaatcaatg gagagacaaa acggccttca atagtttgtt ctacaataaa gacaatcact
    11401 actttcaaaa gcatgactgg accagcaatc aaacccaata ctagggcagg aagcatggct
    11461 agaaagctac caagataagg gaccagattt aaaataccag cagtaacccc cagcgtaacc
    11521 gcatagcgta gaccaataat cttgaagaag atgataaaca ttactgctac aataatagcc
    11581 actgtcactt gccctcgaac atagttggac aactgttgat tcacatctga tagaacttgt
    11641 ccaacaggtt ccttcaattt tcttggaatg aattgggtca aatagttacg caagcctttc
    11701 ccatcacgca agagataaaa gagcatgaaa ggaacgataa tcaaggcaac aatcacttga
    11761 gaagctccgc taataaaggc actcacccag ttgactgcct gagatgaaac cttacttgcc
    11821 aaaactgtag cctggctaga aaaattggtc aaaacttgct ctaattgagg tctgaaatca
    11881 tctggcaggt gctgggctac caatccatta acaatcctat ctatatcttc taagtaaaca
    11941 ggaacgtttc ttgcaaaggt caaaacctga cgttgcagat ttggaatggc gactgccaag
    12001 ccccaaatga taaagagagc gatgataaca aagacaatag tgatagctat aacacgatta
    12061 accttatgct tctccatcca atcaacaata ggattcaaca aataatataa caaaccagac
    12121 aaaatgactg gcaacatcac aactgctaaa aagtctaaaa caggtgaaaa tagaaaacta
    12181 atcttactta aaataaaaag attcagtccc aataataagg ttactaaaaa taccgtaatt
    12241 gccttgttat ctaaaaacca cttgaaaaac caagataggc taaaatgttt ctctttttgc
    12301 tccataaata cttcctttct attgtcctct cattatacca tttttgaaac aaaataactc
    12361 ttttttaaag tgcttacatc taattggata tctttaattt acttgttttt gtgatataat
    12421 agtcttatct ttacacagag gtatagtaat gaaagaaact gtttattttg gaacttatac
    12481 acgtcgtact tctcaaggga tttacaaggc agactttgat acagaaactg gtcagctttc
    12541 aaatctagaa ctttttgcag ctgagccaag tccaacctac cttgcctttg accagcacca
    12601 acatttatac actgttggta gccaagacga taagggggga attgcagcct atcaaactga
    12661 cgggactgtg ttaaatcatg ttgttgaaga aggagctccc cactgttatg ttgctgtcga
    12721 tgaaaagcgt gatttggttt acgcagccaa ctatcacaag ggacaagtcc ttgtttataa
    12781 acgccaggaa gatggtagtc ttctacttag tgatatggat caacacagtg gccaaggtcc
    12841 acatgaaaat caagcttccc cccatgttca ctatacagat ttaacacctg accactatct
    12901 agtgacctgc gacttgggaa ctgaccaagt catcacctat gacctcgatc aagaaggaaa
    12961 attatctaag ctctatacct atcacagcaa gccaggagca ggctcacgcc atatcatttt
    13021 ccataaccac tataaaatcg cttatctcat ttgtgaactc aatagtacta tcgaagtttt
    13081 aatctacgat ggcgttggcg aatttgtacg tatgcaggtt atttcaactt taccagaagc
    13141 ttacgaaggc tttaatggaa ccgctgctat tcatctctct aaagacggta aatacctcta
    13201 cgcttctaac cgtggccatg attctatcgc agtatatacc atccttgcgg acggtagctt
    13261 agagttatta gaaatcgttc caacgcatgg tcagactcca cgtgattttg atttgacacc
    13321 cgaccaaaaa tttctcattg ttgtccatca agactctgac aatgcaactg tctttaaacg
    13381 taattgtgac aatggtcgtc tagcagaact ctccaacgac ttccatgttc ccgaagcagt
    13441 ctgcatccgt tttgctcctt aaaacaccat aaaaaatgaa cttggtattt tcaagttcat
    13501 tttttcttat agtctatttc cgacattaat acggttaatg gcacgttgca aagcaatctt
    13561 agcacgacgt tcttggtcaa tcaaatgttt gtcttgtgct tcttcaattg cacgttctgc
    13621 acgaagtttg gcacgttctg cacgactgat atcgatatca cgagcacgtt ctgcagagtc
    13681 agcgacgatt gtgatcatat cattggcaat ttcaataacg ccaccgttta ctgcaatcca
    13741 gttcacgtga tctttatcat cgatacgttt tacctttact tcatcaactg ctaaaaccgc
    13801 aatcatattt tcatgtcgtg gcaagatccc catctcacca tccagagttc gaaccgatac
    13861 atagctggca tggtgatcat agacgagacc atctggtgtc acgatctgga cagttaactg
    13921 agccataggt cacctcttaa aatcccattt tttcagcttt tgcaatcaca tcttcgatag
    13981 aacctacacc acggaaggca tcttctggca agtggtcgta tttaccatca aggatttcct
    14041 taaagccacg tacagtttca gcaactggaa cataagaacc tggctgacca gtaaattgtt
    14101 ccgcaacgtt gaagttttgt gacaagaaga actggatacg acgggcgcga gcaaccaagg
    14161 tcttttcttc atcagaaagc tcatccatac caaggatagc aatgatatct tgcaattcat
    14221 ggtaacgttg aaggacacgt tttacttcag cagcaactgc atagtgctct tctccaacga
    14281 tttcaggtgc caaggcacgt gagcttgaag caagtgggtc aacggctggg tagataccca
    14341 attgtaccaa cttacgttcc aagtttgttg ttgaatccaa gtgagcgaag gctgttgctg
    14401 gcgctgggtc agtatagtca tccgctggca catagatagc ctggatagag gttacagaac
    14461 ccttcttggt tgatgtgata cgttcttgca attgacccat ttccgtagca agtgttggtt
    14521 ggtaaccaac ggctgatggc atacgaccca aaagggcaga tacttctgaa ccagcctgag
    14581 tgaaacggaa gatattatcg ataaagagaa gcacgtcttg gccttccaca tcacggaagt
    14641 attcagcgat tgtcaaacca gtaagggcaa cacgcatacg tgctcctggt ggctcattca
    14701 tctgaccaaa gaccatggct gttttctcga taacgcctga ttctttcatt tcccagtaaa
    14761 ggtcattccc ctcacgagta cgttccccaa caccagtaaa tactgaaata ccaccgtgct
    14821 cttgggcaat gttgtgaatc aattcttgga ttaagacggt tttaccaact ccggcaccac
    14881 cgaaaagtcc aactttacca cctttaaggt aaggggcaag aaggtcaata accttgatcc
    14941 ctgtttcaag gatttcagaa gaggtagaca actcatcaaa agttggagct tttttatgaa
    15001 ttggctgacg ctctgcgtct tctgtaaaag gagcttccaa atcaatggta tctcccaaaa
    15061 cgttgaagac acgtcccaaa gtttctttac ctactggtac agagattgga cgacccgtgt
    15121 ccaatacttc cattccacga gtcaacccat ctgttgattc catggcgata gtacgaacca
    15181 taccatctcc taactccaag gctacttcaa ggacgatttt tgtttttctt tcgtcatttt
    15241 tgtagacgac aagtgcattg ttaatctcag gaagtttttc ccctgctgca aataaaacgt
    15301 ctacaacggg accgataacc tgagcaattt tacctgaact catctccttc tcctattcta
    15361 tataagatac tgtcggttcc tagctcaact aggtcctaag ttcattttca tacgagctgg
    15421 actagagcct attctaaggc actagcacct gctacgattt ctgtaatttc ttgtgtaatc
    15481 gccgcctgtc tggcacggtt atactgaatt gtcaaatcat tgatgacttt cttagcatta
    15541 tctgtcgctg tttgcatggc tgtcataccc gcagcattct cagctgtctt ggcatcgata
    15601 atggcaccgt aaatcatact ttctgcaaac tgaggcaaca actgctccag aatttcttct
    15661 cggctggttt ccaattcaaa agtcaagctg tactcttcat ccgcttcatt tggatccaag
    15721 tcaacaatcg gaagcatttg ttccacacgc atttgactgg ttagcgtatt gacatggtgg
    15781 ttgtagcaaa cataaagctc atcaaagagt tcattttggt acatttcaac agtttttgaa
    15841 ataatcttac gaacttgatc aaagctaggt tggtctgcca agccacgtaa ttcataaagt
    15901 ggttgaatac cgcgagcctt aaagaaatca gctcccatcc caccgataca gatcatttca
    15961 aaacctttac cgtctgggtg gtattcttct ttcaactcca taacagcttt caaaatagag
    16021 gaattataac ctccaaccaa accgcggtct gaagtgataa cgatatagcc tgtcttcttc
    16081 acagaacggc taatcaacat cggattagtt gaagcaccag ctccattacc atgaaggata
    16141 tctgtcaaaa gtttacgcac tttctgagcg taaacttgga agttgcgagc agcttcttca
    16201 gaacgaccta gcttagcagc cgataccatt tgcatggcat tagtgatttg actcgtattt
    16261 tttgttgagg cgatttttgt tttaatatca tttagagata ctgccatctg acacctctat
    16321 tcttattgga agctagattg attgagaaac tctgtaatcg cagcatccaa gactgcttct
    16381 tctggcaagt cttttgtatc acgaatggtt tccaaaatct ctggatgttg agcatcaaag
    16441 aaggtatgga actcttcctc gaaacgaaca atatcatcta ctggaacagt atccaagaaa
    16501 ccatgtgtca aagcataaag aatggttact tgtttctcaa caggtaatgg tttgtgaaca
    16561 ggttgtttca agacctcaac ggtacgacgt ccacggttca acttagcctg tgttgctgcg
    16621 tccaagtcag aaccaaactt agtaaaggct tccaactcac ggtatgaagc aaggtcgata
    16681 cgaagtgtac cagcaacctt cttcatggct ttgatttgtg cagaaccacc tacacgagat
    16741 acagatgaac ccgcatcgat ggctggacga atacctgcat tgaagaggcc atcgccaagg
    16801 aagatttgtc catcagtgat agaaatcacg ttggttgcga tataggctga gatatctcct
    16861 gcttgtgtct cgataaatgg tagggctgta attgatccac caccaagttc atcagaaact
    16921 ttagctgagc gctcaagcaa acggctgtgg agatagaaaa catcccctgg gaaggcttca
    16981 cgacctggag gacgacgaag caagagcgat agttcacgat aagctaccgc ttgttttgat
    17041 agatcatcgt atacaatcaa aacatgctta ccttgataca taaattcttc cgccatagca
    17101 accccagtat aaggagctag gaagagcaat ggagatggtt gtgaagcaga ggctgtcaca
    17161 acgattgtgt agtccaaggc accgtactga cgaagtgttt ctacttgcgt acgaactgtt
    17221 gattcttttt gtccaatcgc gacgtagata cagatcatat cttgatcttt ttggttcaag
    17281 attgtatcaa tcgcaatggt tgttttccct gtctgacggt caccgataat caactcacgt
    17341 tgaccacgac caatcggtac aagggcgtca atagctttca aaccagtttg caatggttct
    17401 gaaacagact tacgttgcat aacaccagga gctggtgctt ctactggacg agttttatca
    17461 gtgtggattt ctccaagacc gtcaactgga cgaccaagcg gatccacaac acgaccaatc
    17521 agactttcac ctacagggac ttccatgatt ttccctgtac ggcggattgt atcgccttca
    17581 cggatatctg taaagtcacc taggatgata ataccaacgt ctgttgactc caagttttga
    17641 gccataccat aagagccgtt ttcaaaattc aacaactctc cactcatgac attttcaagg
    17701 ccatgagcac gcgcgatacc gtccccgata taggttacaa cacctgtttc agtcacatca
    17761 aaattgggtt tgaaattttc aatttgttgc ttaattaaag cgctgatttc ttgtgcgtta
    17821 attgccaaaa gaacaccact ttctatttca aattttcttt aacaacttta agttgttgtt
    17881 taatactcac atcaattgtc ttgtgattgg caaaaatgac aaaaccacca atgagacttt
    17941 catcgatttg ttcttttaca ctccttactt tcagagacat ttttttctca atcaaaggga
    18001 gcaaacgagt cttctgttca tcagttagag gatgagcaga cgtaatcgtc acttcaaatc
    18061 gatttgtttc tttttcaagt cggttcaagc aatctacaag cacatcataa aaaagatttg
    18121 ctctgtgatt gtaggccaga acctggataa agttttgtaa taaaggcgac acagaatctt
    18181 ggaaaaaagc aattgttttt tccttatccg actcgtctac tgccacttgt tttaaaaaag
    18241 aaggcagacc tgttttttca acaacttgct tgatttgagt caagtctgaa aagatacggt
    18301 cttcttctcc tttttcaagt accaattgga caaaaggcat gctgtatttt tcaattacct
    18361 ttactgtttt cttgtccatt aagcttctcc tagctgatcg atatactgat caatgagtgc
    18421 tttatgggca tgactgtcaa ggttttgtga gatgatttta ccagctaagc tgatggtcaa
    18481 atctgcgacc tcacccttaa cactctgtaa agcttctact ttattttgag caatttcttg
    18541 attggctttt tcttttaagt gtcctgcttc tagtttagca tctgctaaga tattagcctt
    18601 actttgctca gctgtttcct ttgcattttc aatgattgtc ttagcttctt tacggctacc
    18661 agccaattca tcttcgcgtt tttgagccaa tacttctgct ttttgacggg cttcttcagc
    18721 tctgtcaata tctgaagcaa ttttttcagc tctttcttcg aaaatgcctg taatattaga
    18781 ccatgcaaat tttttaatca agactagcaa aagaataaaa gagccagtga ttaaaataaa
    18841 attaccaatt aattcaccta ctgttacgtg catcagttac tcctttctat tcctcttcaa
    18901 tattaatctt attccctaaa tacacagatg tcaaaagagt aaaaacataa gcttggatgc
    18961 aggaaataaa gacagaaaat gcagtccaag ccaaattagc tccaaaggct actggatacc
    19021 aataaatagc ttggtgggaa agaagaagta acaaactcgt catgacctct cctgcaaaga
    19081 tattcccaaa aatccgcaaa gccaaagata agaagtttgt aaattcttcc aagatattca
    19141 tcggtatgac aaaaacagga ctcataaaag attttatact ttttttaaat ccacgacgac
    19201 gaacgctttc tatatgtgtc aacaaaatga caagaaaaga taaggttaag tcatactgta
    19261 aattagcggt tggcgaactc caccagttag tcccatcgat cgtttgaagc tttgtcatta
    19321 agccaaggtt attggcaatc accatgaaaa ggaataaaca aaggaaaaag agtgagtaat
    19381 ctttcatgta gcgcgaacca atgttaggtt ctgtaaatcc aataacaaag tcatagacat
    19441 actcaagtac attttgcttt cctttgggtt tcaaggtcat attgcggctt gcccaataaa
    19501 taaagacaaa aataactccc acaatcaaca aagtcatggc taacatagtc agattgaaga
    19561 taacaggacc aatagagatg attggattaa tactttcttc catctaaaat cctccctttt
    19621 cttctcgttc catgtattta tttgataata aatgagaaga caagagttac aaagaaagtt
    19681 ccttcaataa aggcaacacc taaaaacatc aaactacgaa actcagaaag catatctggt
    19741 tggcgtgcta ctgatttaaa cagtccattc atcaataaac cttcaccgac agatacgccc
    19801 atacaggcaa tacataagcc taaaaatgtt aaattcataa caaattctcc tttaaaataa
    19861 aatttactta cttagtgtag tcctaaaatt taaatttgtc aacttttaac cgctattgga
    19921 agcgtttaat aaaaattatt tccattt
//
LOCUS       DAVBDG010000036        19447 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBDG010000036 DAVBDG010000000
VERSION     DAVBDG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19447
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.036"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(570..1343)
                     /locus_tag="VQ526_001314"
     CDS             complement(570..1343)
                     /locus_tag="VQ526_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2555031.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            1519..2922
                     /locus_tag="VQ526_001315"
     CDS             1519..2922
                     /locus_tag="VQ526_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2555032.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEGTAVGNIVVQLLSQGKINEDTELK
                     DIMTNIADTKYYYPQLS"
     gene            2951..4243
                     /locus_tag="VQ526_001316"
     CDS             2951..4243
                     /locus_tag="VQ526_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2555033.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYSAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVHTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
     gene            4330..5259
                     /locus_tag="VQ526_001317"
     CDS             4330..5259
                     /locus_tag="VQ526_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2555034.1"
                     /translation="MAQKIMSLQNRKNQKRRFIFLFLLPTLICFFLFYFYSVVTIFLT
                     SFAKWDYTNLNTPEFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAV
                     SVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFK
                     DSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYI
                     TIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYAR
                     ANTIGVIQILGGMLIIVCINILFRERKRLKGGK"
     gene            5262..6101
                     /locus_tag="VQ526_001318"
     CDS             5262..6101
                     /locus_tag="VQ526_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2555035.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            6098..7417
                     /locus_tag="VQ526_001319"
     CDS             6098..7417
                     /locus_tag="VQ526_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2555036.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKVTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            7423..9294
                     /locus_tag="VQ526_001320"
     CDS             7423..9294
                     /locus_tag="VQ526_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HET2555037.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKHLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKKMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTVVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDIQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            9316..11532
                     /locus_tag="VQ526_001321"
     CDS             9316..11532
                     /locus_tag="VQ526_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2555038.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSRFDEFFYGDELMNAGIKVSLSNLALCVPEYFTKLFVIEEVVCKY"
     gene            11543..14560
                     /locus_tag="VQ526_001322"
     CDS             11543..14560
                     /locus_tag="VQ526_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HET2555039.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINSNGNLEFELHF"
     gene            14575..15303
                     /locus_tag="VQ526_001323"
     CDS             14575..15303
                     /locus_tag="VQ526_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET2555040.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            15316..17082
                     /locus_tag="VQ526_001324"
     CDS             15316..17082
                     /locus_tag="VQ526_001324"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2555041.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            17228..18379
                     /locus_tag="VQ526_001325"
     CDS             17228..18379
                     /locus_tag="VQ526_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2555042.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVANGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            18524..19408
                     /locus_tag="VQ526_001326"
     CDS             18524..19408
                     /locus_tag="VQ526_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2555043.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         6484 a         2873 c         4016 g         6074 t
ORIGIN      
        1 cttactatct agccatctgc agacgaaata caagataatt ccagctataa catccgctat
       61 aatagtaaga gcgagctcta tgataaggct cattagtttc acctcctctc acgaacccat
      121 aggaacgtaa tcggtaaccg atgacaaaaa tagtatacca caatacattt agatcatcaa
      181 ggtcgcttaa ttcttgaaat atagggtcta ggcgacaaag tctttaaaat cagaaaaacg
      241 cataatatca ggtgtgcaaa aacttgatac tatgcgtttt attgtgggaa ggtttactcc
      301 attttctcct gaaattgagt ttttgtccag tctctgtttt tagggttgct aagaaaataa
      361 tgtcatgtgg tgaatatttg taaatcagtc agcagacaga acgaatactc ttcgaaaatc
      421 ttttcacatc atgtcagctt cgtctttccg tatatatgtg actgacttca tcagttctat
      481 ctacaacctc aaaacagtgt ttcgagctga cttcatcaat tttcaaatct gtactttgag
      541 caagctgaga ctagcttcct atttgatttt cattgaatat cagaaaccca ttctccatca
      601 aataattcga ctgcgtctaa taatttttgg tctggcaagg tgtcagaaat aaaggttgtg
      661 tatttggata ggggattaat tttaaaaaat ccagtcttgt aaaatttaga actatcaatc
      721 agtaagatgg tttcatgggc tttgtcaata atattctttt ttgaaatagc ttggctgaga
      781 gaagcttcat aaacatattg gtcatcaata cctcttgctg aacaaaatgc taaatcgata
      841 ttaaaatgat ctaataaaga attttcctta tcatagttga ccacggaaca ggattgatgt
      901 ttgacctcgc cagatgtgat aaagattttg gagctatctt taacagtttc agatagggtt
      961 tgtgcagtat gtaaaccatt tgtaaaaata atcaaattat caagttcaga aagataggga
     1021 caaagttcgt agacagtagt actagaatct agatagatac acataccaga ccgaataaag
     1081 tctttagcga gactagcgat tagtcttttt tgcctagtac tttctccttc acgtatttga
     1141 tgagaaagtt caattgtgtt catagaggac agggtcacgt atccgtgctt tcttttgata
     1201 agaccttgat tttctaagaa aattaaatca cgacgtaagg tacttgtgct ggagaaagtg
     1261 atttctgcca gctcttttac ggcaattctt tttttctttt tgataatttc aatcaattca
     1321 agtacacgtt catcttttat cataagctcc tcctaattta tcatttcaac tatattatag
     1381 cacaaattgg aggaatgtga attattttta tgaatattgg gttaacattt gaacattatt
     1441 caagtaagcg ttcacatatt gaaaaaataa aacgtgggga ttataataaa gttaatcaag
     1501 gacgaagaga gaagaaaaat ggaagcggtt ttagcaatag atttaggtgc gacttctgga
     1561 agagcaatcg ttggttacct ttctgaaaat aaactagtaa tggaagaaat aaatcgcttt
     1621 tctaatctac ctattagagt aaaagggcat ttatcttggg atattgactt tctactagct
     1681 aaaattcttg aaagtatccg cttggctaat actagttaca agattttatc tatcggtatt
     1741 gacacatggg gagttgattt tggactgatt gataatgaag gtaagctgtt atcacaacct
     1801 gttcattatc gtgatgaaag aacaaaggga gtgttaaagg aaatatctga aatgactgaa
     1861 ttagaaaaac tgtattcaga gacaggaaat cagattatgg agataaatac cttgtttcaa
     1921 ctctttaagg cacgtcaaga atctcctgac tctttctata agaccaataa gattctttta
     1981 atgccagatt tgtttaatta tctcttgaca ggtaagtttg ctacagaaaa aagcattgct
     2041 tcaacaactc aattatttga tcctaggagt caaaattgga atcagaatat cttaaaacta
     2101 tttgaattgg attcatcttt acttcctgaa attgtttcag agggaaatgt tcttggaagg
     2161 ataaaagagg agtatggttt aggcgatatt cctgttgtga atgtttgtag tcatgataca
     2221 gcaagcgcga ttgtctcagt acctaagaca gaaggtagtt tatttatttc atcaggtact
     2281 tggtctttgg ttggagtgga acttacttca ccgattctta ctaccgaatc cttcagttat
     2341 ggatttacaa atgaagtcgg taaagatgga gtgattacat ttctgaagaa ttgtacaggg
     2401 ttgtggatca tagaggaact aagacgttca tttgaacgaa gagggaaagc ctattctttt
     2461 gatgatatta ggacaatggt ggagaaagaa aaagaaaatc ttcctctgat tgatactgaa
     2521 tcaactgaat ttgcaacaga atctgatatg cacaagactt tgacagaata tctagcttat
     2581 catcatgaaa ctagagagtg gacagatgga caactattta agattgttta tgaaagccta
     2641 gctgaaacgt ataggaaagc gatagagtta ctagaagaac taactcataa ggtttataag
     2701 aggatatatg tgattggagg aggtgctaga gccagttact ttaaccaaat gattgctgat
     2761 agaactggta aagaggttct tacaggtttg actgagggta cagctgtggg gaatattgtt
     2821 gtgcagctat tgagtcaagg taaaataaac gaggatacag agttaaagga tattatgaca
     2881 aatattgctg atacaaaata ttattatcct cagctatcat aaacaaaaat aaaaaaacgg
     2941 aggttttgtc atgaacaaaa aaagtctttt gaaatgcgct gttatcgggt tagtagctac
     3001 ctttggttta gcagcttgtg gaacatctaa ggatgcaagc ggtggaagtt cttctggtaa
     3061 agaagtatta gaattttatc atgggtatca tcatagtgaa gatgaatggc ctgtagcgaa
     3121 gactatgcga gatttatatg ataaatttgc agaagaacat aaagatagtg gtgtagaatt
     3181 taaaccaact cctgtaaatg gtgatttgaa ggatataatg aataacaagg ttgctagtgg
     3241 tgaatttcct gatgtgattg acctagcagg taatgcagta tcattagctg cgattgaaca
     3301 aaaacttgtt ttagatttga aaccatatat cgattcaaat aagcttgaaa aaaatgttgg
     3361 tttgaactat aagcaaaacc aaaaagatgg aaaaatttat acagttcacg aacagttatt
     3421 tacaatgggt ttgtggtaca acaaggacat atttgcaaaa gcgggtgcta agacacctga
     3481 tcagtggaat acttgggatg atttcactca ggcaatggca agcattagaa aacaagatgg
     3541 agtgtatgca tttggtgctg gagaaccatc tattcgcttg tttaatacag tattaggaac
     3601 cacagaaaat ggacgtaaat tattagataa accattaaca aaagaaggaa ttgaatctaa
     3661 agaatttgca gatgctttga aaatggttat gaaagaaatt caagctaatg gttctaagaa
     3721 tgctggtggc gatgccaatg cttattccaa agatttccaa gaaggtaaat ctgcagtatt
     3781 ctttaatggt gtgtgggctt ctggtgagat gtctaagaac ccaagtcttg cacctggaat
     3841 ctattctgca ggtgtagcaa ttagttcttc tggaggtggt atcactatct caagtaagat
     3901 gtctgaagct aaacaaaaac tagctcttga gtttttgaaa tatatgacta gcgatgatgt
     3961 acaaaaagta atttttgaaa aagtaggagc aaatccttct aatgaaaatg taaatgtaaa
     4021 agaactttca gaaaagagtt cagaagctac tactaaaatt ttaggacaag caatcactca
     4081 agttaagaat gcaaaagcag ttgttccaac tgtaagtgat gtttggggtg gcgatgtaca
     4141 cacagctatt atcaatgcat tgacagaaag tgctgctgaa aatgtagatg ttgatcaaaa
     4201 agtaaaatct actcaagatg tgttgaaatc attgattggt taattgtttt agggagtgaa
     4261 aacgaagctc agttggcact gccaactgag cttgatttta cttatatagt tgaaaaaagg
     4321 agtttgttta tggcacaaaa aattatgagc ctacaaaata ggaagaatca aaaaagacga
     4381 tttatctttt tatttctttt gccaacgttg atttgtttct ttctatttta tttttattct
     4441 gtagtaacaa tctttttaac ttcatttgct aaatgggatt atacaaattt aaacacccct
     4501 gagtttcttg gatttgataa attatttgaa aattataggt atgtttttaa agaataccca
     4561 ttttttacag aagctctaat taattctgtt cgttgggctg taattggtgt tattattcaa
     4621 gttcctttag ctgtatctgt tgcaattaca ttatctaaga aattaaaagg ctggaagata
     4681 tctaggaatc tttatattgt accaagtatc atttctagtg ctgctatggg cttgattttt
     4741 cttcaaatct ataatccaaa ctatggtgtt gttaaccaaa ttattcatct atttaatcca
     4801 tcgtttaaag attcagtact gttgactcca ggattaaata tagtagctat gactggcgct
     4861 tatatcttct ttgcaggagc atcaaccatt atgattcttg ggcaaatttt tgctattcca
     4921 gaagaagttc aagaagctgc tattttagac aatattactg gttggaggaa agagtggtat
     4981 attacgattc cgatgattaa ggggacaatt aaaactgttt caattatggc agcaacttca
     5041 ggatttttgc tctataacga agtattcttt ttgacaaatg gtgctgcagg aacaaaaagt
     5101 atcagttttg ttattcgaga attagcagtg gctagctcac gaactcagta tgctcgtgca
     5161 aatacaattg gagttataca aatcttaggt ggaatgttga ttatcgtttg tattaatatt
     5221 ttattcagag aaagaaaaag actgaaaggt gggaaatgat tatgaataca catataaatg
     5281 gtattagtaa aaaaggcaaa gttcttatat atggttatat gctccttacc attttaattt
     5341 ctattttccc tattgcgtgg atttttttat catcattaaa agcagatcct atgaaaaatc
     5401 caggtattag tttaccgact gactttactc ttgaaggtta tataaatgtt tttacaaaac
     5461 ttcatgtttt tacttacttt tggaatagct ttaaagtagt gtctatatca gttattatta
     5521 gcattgttat gatctccatg tcttcatatg ttattgctag gatggaattt agagggaaga
     5581 agttggtgac ttctatgctg tattctactc tatttattcc agcaacagct atgacttttc
     5641 cagtttatag attagtaaat gagttgggga tttataatac cccagttgct ctaattctgg
     5701 tctactcttg tagtggaatt gctatgagtt tctttattat aaagaactat tttgaaatta
     5761 ttccaaaaga attggaggag gcagcagaga ttgatggtgc gacatatgct caaacttttt
     5821 ggaaagtgat gttgccaatt gctagaccag gaattttaac agcagcagta cttgccttta
     5881 ttaataactg gaatgaatat tattgggcat ctatgttggt gattgataaa aatgaattaa
     5941 cagtaccagc attactaggt caatttacta caagttttaa tactaattat aatggtctat
     6001 tttcagctat tgttgtaatc gtattaccac caatcattct attcgcattt acaagtaaat
     6061 acttcattga agctttagga ggaggagcag taaaaggatg aaacatacac ttgaaactat
     6121 caatagtaga actcagtggt ttagagaagc aagatttggg atgttcattc attgggggtt
     6181 atattctatt ccaggaaaag gggaatggat tcgtagtcat cagaaattat ctattgaaga
     6241 ttatgaacca tattttcgag cttttgatcc taaggaatat aaccctagag aatgggctaa
     6301 acaagcaaaa gctgcaggga tgaagtatat ggttttaacg gctaaacatc atgatgggtt
     6361 ttgtttgttt gattcaaaag ttacagatta taaagcaaca aatacacctg caggtagaga
     6421 tttagttaaa gaatttgtag atgctgttcg agctgaaggt ttaaaagtag gcttatattt
     6481 tagtttgatt gactggcacc atcctgattt ccctaaatat gctgatctaa atcatccaat
     6541 gagaggaaat gaagcctatc gagatgagaa aattaacttt gacagttatt tggagtattt
     6601 gcacaatcaa gttaaagaaa ttgtaactgg ttatggtcag attgacatat tatggtttga
     6661 ttattcttat gaagacatgg tgggtgaaaa atggggagct tcaaaattaa tagacatggt
     6721 tcgtcattat cagccaaacg ttatcgtaga taatagatta gaaacatcag gtgaaggatt
     6781 cggaagtatc gttacagatg aaattacctc ttacgctgga gattttgtta gtccagagca
     6841 aattgttcct cacgaaggta ttcgaaactt taaaggtgaa cctgttcctt gggaactttg
     6901 tctaactatg aataacaatt gggcttataa tcctacagat tatctatata agtctagtca
     6961 aactttgata agaaagttag tggaatgtgt tagtaaaaac ggaaatatga ttcttaatgt
     7021 aggcccagat gctttgggaa gaattaatga ttcaagtaag aaaatattag ataattttca
     7081 tcgatggatg tctcgaaatg gtgaggcaat ttatggatgt agtggagatg aaaatttacc
     7141 aaaaccagat tggggatact atactcgtaa tggaaatacg gtttatgctc atgtatttga
     7201 acaacctata ggtcctttag ctcttcttgg tattagtaaa gaaaacgtaa aaagaatgag
     7261 ctttcttcat gatggaagtg aggtgaaaat ttcagaaagc tggacgacca atgcctacaa
     7321 aggaatttgt tttgcacagt ttggagaagt tccacatttt acctatccat taccagactt
     7381 gattgatagc gtaatcaaaa ttgagttgag agagtagttt atatgaaaat tgaaaataaa
     7441 aatgtaagac gtaatttctt ttggggagat ggtcgatttt atactactga tatcgtaaac
     7501 aaacgggcag gagtaatgat aaaaaatgtt tcgaaagaag aatttaccat tacattggag
     7561 aatggtataa gactttcttc aaatcatttt tcagcaatcg ttagggaaga gggagatacg
     7621 cgaattcagg tttcttttgt ttgtccttcc attcgtttac gtttaatctt tgaatcgagg
     7681 gatgatgttc tttcgaagca attagtttta gaatcatcta ctgaggttat taaatctgta
     7741 gaggtagaga gttttgagtt tgaaacagaa gacaatattt tctatccgaa aagacaagat
     7801 tgtattaagg aaatggcgaa tttttccggt tattatgtag aattgggaca acctgtttat
     7861 gcgaattctt tattcttagg aatggaattt ccaatgtctg aaaacaaggt agatggtaga
     7921 cactatgtat caagatatta cttgggaact gttgtaaatc aagaaaagag tttgtggtct
     7981 tgtattattg ggggagcatg ttcttataaa aaagaagaga ttcaagaggc attttttgaa
     8041 tatgttgaag gaatagctca acctagttat ttccgtaaac agtataattc ctggtatgac
     8101 catatgaccg atattacaga ggaaggtatt ttaaaaagtt tttctgagat tcgagatgga
     8161 tttgaaaatc atggagttca tttagatgct tatgttgttg atgatggttg gacaaactat
     8221 caatcagttt gggaattcaa tcataaattc ccaaatggtt tgagaaatat taaacatctt
     8281 gtaaatggat ttggttcaag cctaggattg tggattggtc cccgaggtgg ttataatggg
     8341 acagaaatca ttatgagtga ttggttagaa gcacatccag agttaaatat tggatctaaa
     8401 aatttgattt caaatgatgt aaacgtggct gattttaact atctcaatca aatgaagaaa
     8461 aagatgttgg aatatcaaaa agaattcgat atcagctatt ggaaaattga tggttggtta
     8521 cttcaacctg acaaacctga taagagtgga ccgcacggta tgtataccat gacagtggtt
     8581 tatgagttct taattcaact gttgatagat ctaagaaagg agagaggagg aaaagattgt
     8641 tggttaaact tgacttctta tgtaaatcct agtccatggt ttttacagtg ggtcaatagt
     8701 ttatggattc aaatatctca agatgtaggc tttacagaga atgcaggtaa tgatatcaat
     8761 cgtatgataa cttaccgaga tattcagtat caagaatttt tggaaaaacg tgagatacag
     8821 ttacctatgt ggtcgcttta taatcatgaa ccaatctatg ctgtcagtgc aaatacctgg
     8881 tacatggatc atcaaatgtt tgcatcaata ccagattttg aagcttatct attatttatt
     8941 tcaacaagag ggaatgcttt ttgggagttt cactattctt ttgatatgtt tgatgaagaa
     9001 cgctggaaag ccaatgctcg tgccgtcaaa tggatagagg agaattatca aacactaaaa
     9061 tatagtaaaa aaataggagg aagtcctgaa aaatttgaaa tttatggtta taagtgtcac
     9121 aatcagaaga cttctactga aattctctct ctgagaaatc ctgctcagat taaacaaaaa
     9181 ataaaaatag agaatctttc gatagagaat tttaccagag taattggaga ttttactatt
     9241 caagaagatg agatagaatt ggcaccgtac tctatcgtga tactgaagaa gtaaagaaaa
     9301 aattgtgagg tagagatgac gatttatatt aataaggacg agaccgtttt tcatttggca
     9361 atgaaagata gtagttatat ttttagaatt ttagaaaatg gggaacttca acatctacat
     9421 tttgggaaaa ggattcatgt caaggaaaat tataaccaat tgatggccta tgaaaaaaga
     9481 ggatttgaag tatctttttc tgaagaattt gaggatattc aacagtctat gatacaaaat
     9541 gaatattctt catatgggaa aggagatttt cggcatccag cctttcaagt tcaaggaatg
     9601 aatggtagta ggataacgac actaaaatat caaggttttg aacttgaaaa agggaaaaat
     9661 cgtcttaact ctctaccttc aacatttgat gatattggtc agtgtgcgga aacattaacg
     9721 attattttaa cagattccat attagattta actgttagac taaattacac aatttttccg
     9781 gaatacaatg tcttagttag aaatacggaa tttttaaata atagcaataa taagttgact
     9841 cttttgaaag caatgagctt acagctagat ctacctgata gtcaatatga ctttattcaa
     9901 ttttctggag catggctgag ggaacgtcag ttatatagaa cttcgcttag accaggtatt
     9961 caagcaatag atagcttgag atactcatca agtcctcagc aaaatccttt ctttatgcta
    10021 tcaaggaggg aaactacaga gcatagtggt gaggtttatg gttttaactt tatctattct
    10081 ggaaattttc aaaatatgat tgaagttgac cattttgaca ccgctagagt aacggtagga
    10141 ataaatccag tagaatttcg ttttttatta aatcctgccg aaagttttgt gacaccagaa
    10201 gcaattgtga tctattctga tcaagggatg aatcagatga gccaacaact atcagatttt
    10261 tatcgacatc atttagttaa tcctaatttt tctcaagcta gtcgtcctat aatactcaat
    10321 agttgggaaa cattttattt tgacttgagt acagaaaaaa ttttagattt agcaaaggct
    10381 gctaaagatt tagggataga attatttgta ctggatgatg gttggtttgg tcataggaaa
    10441 gatgacaaaa gttctctggg tgattgggta acagatagaa gtcgccttcc tgaaggtatt
    10501 ggatttcttg cagatgaaat tcacaaaata ggtttacaat ttggtttgtg gtttgagcct
    10561 gaaatgattt ctattgatag tgatttgtac aagaatcatg ccgattggac tatccatttg
    10621 ttagacagag agaagtcagt aggaagaaat caatatgtgt tggatttgac gagacaggaa
    10681 gttgttgatt acctttttga ttctatttct aaaatcataa tcaaaacaaa tctggattat
    10741 atcaaatggg atatgaatcg tcatataaca gatatttata gtattgaact tgattctgaa
    10801 cagcagatgg aatttggtca tcgatatatc ttaggtcttt atcagttatt agatcgttta
    10861 ataactaagt tcccttcagt tctatttgaa tcttgttctt caggtggtgg acgttttgat
    10921 ttaggactta tgtattatgc accgcaagcg tggacgagtg atgatacgga cccgatagaa
    10981 agattgaaaa ttcagcatgg aacttcttat ggatattctc catcaatgat gacagcccat
    11041 gtttctattt ctccaaatga acaaagtgga agacaaacga gtttggacac taggacaaat
    11101 gtagcttatt ttagttcttt cggttatgaa ttagatgtta cgagattgtc ggtagaagaa
    11161 aaagaacaag tgagagaaca aattcagttt tataaaaaat atcgttcatt gtttcaatat
    11221 ggggatttct ataggataaa cagtcctttt agttgtgatt ctgctagttg gcaagttgtt
    11281 tcaaaagata aatgccaatc gattttattg tatgctcaat tgaatagtaa gttgaatcca
    11341 ggttatacaa gagtttattt tagtggttta gataaagata aatgctattc cgtctctaga
    11401 tttgatgagt tcttttatgg agatgaatta atgaatgctg gaataaaagt cagtttaagt
    11461 aatttagcac tttgtgttcc agaatatttt acaaaattat ttgttattga agaagttgta
    11521 tgtaaatatt gaggaaataa aaatggaaag taaaaaaata gcaaaacaga ttttaatagc
    11581 tacggctgtt ttaacaagtt ttttaggaag taatttggtt tatgcagatg tagttcaaag
    11641 taattctaat aacagagcta gtactgaaac tgctagagta acagggaata acttagaaaa
    11701 acttattact aaagataaag aaattgataa agagatgaca tatctttcag atatggattg
    11761 gtcttctgca actcatggtg atatagataa aacaaaaaca gtacaaaaag atgctccatt
    11821 tacaacagga aataaaggag agcatactaa gatttcattg ctcacttctg atgataaagt
    11881 taaatatttt gataaaggaa taggaacagt cgcagattct ccatcagtaa tcagctatga
    11941 tatcagcggt caagggtttg aaaaatttga aacttatata ggaattgatc aaagtgctaa
    12001 tagttctaga tcagaccatg cagtagtgga tagaatagaa atagaaattg atggaaaggt
    12061 tgtctattct agtagtgtaa ctaatccaga aggattcaga tataataccc aggcacaatt
    12121 tattagtgtg acaattccac aaaacgctaa aaaaatatcc ttaaaatcat tcgcaggaga
    12181 acatacatgg ggtgatgagg ttgtatttgc tgatgctaaa cttattaaaa ctgttagtac
    12241 tcaaactata actccagatc ttcttaataa aggaattaat ggaggagtat atctttctga
    12301 tttagaatgg gttgatgcta ctcatggtga tgacgataaa tcaaaaacag ttcagaaaga
    12361 taaaccgttt acacctggta ataacgggtc taataataaa ataaaattac taattgacgg
    12421 taaggaagtc gaatttaata agggattggg aactgtagca agcaacccgt ctagtataaa
    12481 atatgatgtt tcgggtgcta atgttacaag atttatttcc tatgtcggaa ttgataggag
    12541 cgccaatcat ttaaattcag attacgcaga tattcaaaaa tttgaagttg ttgcggatgg
    12601 taaagtcatt tactcatcag attcaaaata tccaaaagga attaagtatg atacatcggc
    12661 atttctggtt gatgttgaaa ttcccaaaga tacacagaca atagaactta aatcatattc
    12721 agggaaacat acatgggctg acgaattggt tttaggtgga gccttgttta tggcaaatgg
    12781 taaatttaag aatcctaatg attggtcaga agttgataaa cgtcgtgaaa ttaataatga
    12841 acatccgtta cttatgatgc ctttatatgc taacggagaa gagtttaacc aagggaaata
    12901 tactttttgg ggaggagata cattaacagg taagtgggag aatataccag atgacttaaa
    12961 accatatact gttatacagt tacatccaga tgatcttcct aaaagagatg gagctgctag
    13021 agatttttat gaacatatgt tagaagaagc agcgaaatat gtaaatccta agactggaaa
    13081 aaatgaacca attccagtta ttctgacggt ttacactgct ggtaatatgc cttattatac
    13141 ttcggcacat tggttaagta ccagctggat tgataaaatg tatcaaaaat atccaaattt
    13201 gcatggtatt ttcagtacag agaattattg gatttgggca aatgatattg aaaataaggc
    13261 ggcagattac ttaaaagtat cagctaaaaa tggaggatat tttatatggg ctgaacaaaa
    13321 taatggttct gcaatagaaa aagcatttgg aaaaaacggt aagatagcat ttcagaaatc
    13381 tgttgataag tactggaaga accttatttt catgttcaaa aatacacctg ctgctgaggg
    13441 aaatgattca actacagaaa gttatatgaa gggactttgg ctttcaaatc atacttatca
    13501 atggggtggt ttgatggata cttggaagtg gtatgagaca ggtaaatgga aactgtttgc
    13561 aagtggtaat atcggaaaaa gtcaaggaga tagacaatgg ttgactgaac cagagtctat
    13621 gctaggagaa gaagctttag gggtttacct taatggaggt gtagtttata attttgaaca
    13681 tccagcttat acctatggtg taaataataa agaaagctta ctctttagcg aagtaataaa
    13741 agaatttttt agatatgtaa tagcgcatcc agcaccatcc aaagaaaagg tacttgagga
    13801 tacaaaagta ttcatacatg gagactattc aaataaagga aatggtaagt tctttgtaaa
    13861 cgtaaatact gatagagaac aaactccatt atacatgact ggtcgttata atgttattcc
    13921 agctattcca ggtgttttaa aaacagataa attgaaagag tctgtttctg gtagccgtat
    13981 tcagattaag gaaatcactt ctccagaatt tagctctact caggctagaa aagaatactt
    14041 aaataagctg tatcctatga attatgaagg agatatcttt gctcagaaat tagataatag
    14101 atggtttgtc tataattaca aggtaaatga gaatgttaaa cagactggga aattaaaatt
    14161 taattcttta gaaatggatg ttgaatttga acctcatacc tatggtattt ttgagagaat
    14221 aagtaatgga ttgaaagtga atttaaataa ttttagaaca aataaagatt cactatggtc
    14281 taatgctcaa gatgcaaatc aggcaaagaa actaccacaa ttgaccaaga aaggtgcgat
    14341 taagtggatt gaagaacatt atatcaaaga tactcagttt ggagagaaaa gggttacaaa
    14401 gatagtatta agaggtatag ataaacttcc tactattcat tcattatcag gaacaaataa
    14461 ttcctatgat cagccatctc tcaattttga tcagaaaaat catatggtta ctattaccat
    14521 taacagtaac ggaaatcttg aattcgaact tcatttttag aaaggagtga gtttatgtct
    14581 caagatgaaa aattaattcg tgaacagatt tgtgatgttt gtcataagat gtggcaactt
    14641 ggttgggttg ctgctaacga tgggaatgta tctgttcgat tagatgagga taccattctt
    14701 gcaacaccta ctggtatcag caaaagtttt attacaccag aaaagctggt gaagttaaat
    14761 cttaaaggag agattttaga agcagaaggt gattactgtc cttctagtga aattaaaatg
    14821 cacattcggt gctacgaaga acgtgaggat gtccgttcag ttgttcacgc acatccaccg
    14881 attgcaacag gatttgctct tgcacacatt cctttagata cttattcact aattgagagc
    14941 gcgattgtgg taggggcaat tcctataacg ccatttggag ttccgtctac aatggaagtg
    15001 ccagaagcaa ttacacctta tctgcccgat catgatgtca tgctattaga aaatcatgga
    15061 gctctgactg tcggaagcga tgtcattaca gcatactacc gtatggaaac tttagaatta
    15121 gtcgcaaaga caacattcca cggaagaatg ttactttcta caaagggcat tgaggagcaa
    15181 gaaattgctc gtccgacttt agaacgtcta ttctcaatgc gagaaaatta taaggttaca
    15241 ggtcgtcacc caggctaccg taaatataat ggcgatggta gtatgaaaga aacagaaaaa
    15301 taagaggaaa atagtatgat tcaacatcca cgtattggta ttcgtccgac tattgatggt
    15361 cgtcgtcaag gtgtacgcga atcacttgaa gtacaaacaa tgaacatggc taaaagtgtg
    15421 gcagatttga tttcaagcac attgaaatat ccagatgggg aacctgtgga atgtgtgatt
    15481 tctccatcta ccattggtcg tgttccagag gctgcagctt cccatgagtt gtttaaaaaa
    15541 tcaaatgttt gcgcaacaat tacagttaca ccatgctggt gttatggtag tgaaactatg
    15601 gatatgtctc cagatattcc tcatgctatt tggggattta atgggacaga acgcccagga
    15661 gctgtctatc ttgcagctgt actagcttca catgctcaaa aagggattcc agcctttggg
    15721 atttatggta gagatgttca ggaagctaat gatacagata ttccagaaga tgtcaaagaa
    15781 aaacttttac gttatgcgcg tgcagctctt gcaactggct tgatgagaga cactgcttac
    15841 ctatcaatgg gtagtgtttc gatggggatt ggtggttcta ttgtaaatcc ggatttcttc
    15901 caagaatact taggaatgcg aaatgaatcg gtagatatga cggagttcac gcgccgtatg
    15961 gaccgtggta tttatgaccc agaagagttc gaacgtgcta tggtttgggt aaaagaacat
    16021 atcaaagaag gggttgaccg aaatcgtgag gatttgattc tttctaaaga agagaaagaa
    16081 aaacaatggg aatttgttat taagatgttc atgattggac gtgacttaat ggttggtaac
    16141 ccaagacttg ctgaacttgg ttttgaggaa gaagcagttg gtcaccatgc tttagtagct
    16201 ggtttccaag gtcaacgtca gtggacagac cattttccaa atggagattt tatggaaact
    16261 ttcctcaata ctcagtttga ctggaatggt attcgaaaac catttgtatt tgcgacagag
    16321 aatgattcac taaatggtgt gtctatgctc tttaattatc tattaacaaa tactccacaa
    16381 atctttgctg atgtgcgtac ttattggagc ccagaggctg ttaaacgtgt aacgggacat
    16441 actttagagg gtcgtgctgc agctggcttc ttacatctaa tcaactctgg ttcttgtaca
    16501 ttggatggta caggtcaagc tactcgagat ggcaaacctg ttatgaaacc attctgggag
    16561 ttggaagaaa gtgaagtgca ggctatgctt gaaaatacag acttcccacc agcaaaccgc
    16621 gaatacttcc gtggaggagg attctcaact cgtttcttga cgaaggggga tatgccagta
    16681 acaatggtac gtctcaatct tctaaaaggg gttggtccag tgctacaaat tgcagaaggt
    16741 tatacacttg aacttcctga agatgttcac catactttag ataatcgtac agatccagga
    16801 tggccaacta cttggtttgc tccacgtttg acaggaaaag gtgctttcaa gtctgtctat
    16861 gacgtcatga ataattgggg agctaatcac ggagccataa catatggaca cattggagca
    16921 gacttgatta ccttggcttc tatgttgaga attcctgtca atatgcataa tgtacccgaa
    16981 gaagatatct ttagacctaa aaattggtcc ttatttggaa cagaagatct agaatcagca
    17041 gactatcgtg catgtcagtt attgggacca ctacataaat aaaatttgtt tatataggag
    17101 gtgaacttac gtccctccta tccttttaaa aagatttgtt aaacaattca caaataattg
    17161 aaaacgaata caaaaagtaa tataatgatg ttaaatagat agcgcggagg cgcaggagga
    17221 aaattatatg gctacatttt atgttccggc agtcaacctt attggaaaag gtgttgtaaa
    17281 tgaagtgggt ccttatatca aggaacttgg ctataaaaag gcacttttgg ttacagataa
    17341 gtacatcgaa ggcagtgata ttttacctaa gactttaaaa ccactggata cagaaggaat
    17401 cgaatatgtc atctttagcg atgtagagcc aaaccctact tgtaaaaatg tcacagatgg
    17461 ggtagctgct ttgcaagaac atggctgtga ctttatcatc agtcttggcg ggggttctcc
    17521 acaggatgca gctagttgta tttctatcat ggctacaaat ggtggaaaac cacaggatta
    17581 tgaagggctt cataagtctg ctaaaaaagg cttgccagtt gtggctatca atacaacggc
    17641 aggaacttct gcagaaatca ctattaacta tgtgattacc gatgaagaac gcaaggttaa
    17701 gatggtaatg gttgacaaga atagccttgc tcttatctct gttaatgacc cggaactcat
    17761 gctttccaaa cctaaaggct tgactgctgc tactggtatg gatgctctga cccatgctgt
    17821 tgaagctttg gtaacacctg gtgcttatga tgtgaccaag aaactctcta tcggagcaat
    17881 tgagcttatc aaggaatacc ttcctcgcgc tgtagcaaat ggacacgata ttgaagcgcg
    17941 tgaaggtatg gtcaatgcca tcttccttgg tggtatgagc tttaataatg ctggtcttgg
    18001 ttatgttcac tcaatggctc accaactcgg tgcagtatat aatttgccac atggtgtttg
    18061 ctgtgccatg ttgctaccag ttatagaacg tgaaaatgct aaacgtgtac cagaagcttt
    18121 ccgcaatgtt gccaaagcct tgggccttca tgtggaaggt aaatcagatc aagagtgtgc
    18181 cgattatgcg attgctgaga ttgagaagct ttctgagaca gtaggtattc ctaagaaatt
    18241 gactgaactt ggtattgaag aaaaagattt cgactttgaa tacctttcta agaatgcctt
    18301 gattgatgcc tgcgcaccag gaaatccatt tatgccaacc ttagaagaaa cgattgcctt
    18361 ttataaagag ttattttagc aagagattag acgacaatag gacaattcac ctctaattat
    18421 tgacatttca ggaaaaatcg cagtaaaaac ggcacagcct tctttaaatg tgctataata
    18481 ctagaaaaat acttgtggag gttccattat ggcaatattt ttcatgattt ttctgattgt
    18541 ttgtgtgctc ctattggtga tagtcacact gagtacagtt tatgtggttc gtcagcagtc
    18601 ggtggcgatt attgaacgct ttgggaaata ccaaaaggtt gctaatagcg gtattcatat
    18661 tcgcttgcct tttgggattg actcgattgc agcacggatt cagttgcgct tgttgcaaag
    18721 tgatattgtg gttgagacta agaccaagga caatgtgttc gttatgatga atgtagcgac
    18781 tcagtaccgt gtcaacgagc agagcgtgac agatgcttac tataaactca tacgtccaga
    18841 atctcagatt aaatcttata tcgaagatgc tcttcgctct tctgttccaa aattaacctt
    18901 ggatgaattg tttgagaaaa aagatgagat tgcccttgaa gttcaacacc aagtagcaga
    18961 agaaatgacc acttacggct acattatcgt gaaaaccttg attaccaagg tcgaaccgga
    19021 tgcagaagtt aagcaatcca tgaatgaaat caatgcggcg caacgtaagc gggtcgcagc
    19081 acaagaattg gcggaagctg acaagattaa aattgtcact gcagctgaag cagaagcaga
    19141 aaaagaccgc cttcatggtg tggggattgc ccaacaacgt aaggcgattg tggatggatt
    19201 ggcagagtct atcaccgaac tcaaggaagc caatgttggc atgacagaag aacaaatcat
    19261 gtctatcctc ttgaccaacc agtatttgga taccttgaat acctttgcct ctaaaggaaa
    19321 tcaaaccatc tttttaccaa atacgccaaa tggtgtggat gatatccgaa cacaaatctt
    19381 gtcagccctt cgcgctgaga agaaataata gactaatact cttcgaaaat ctcttcaaac
    19441 cacgtca
//
LOCUS       DAVBDG010000037        18782 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBDG010000037 DAVBDG010000000
VERSION     DAVBDG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.037"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(163..966)
                     /locus_tag="VQ526_001327"
     CDS             complement(163..966)
                     /locus_tag="VQ526_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2555044.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1241..1996)
                     /locus_tag="VQ526_001328"
     CDS             complement(1241..1996)
                     /locus_tag="VQ526_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2555045.1"
                     /translation="MSKHPHYELLNLIGYGLAKFAKLFIKEFQYSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNSRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYVEIVKM
                     LLASEGKQTGITIIEKPIIRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDACLY
                     GDGYDFQVDIQEHSYLAEVKGIRKPKGRVRLTAYEFEKAKEFQSDFILSLVTNLDDIP
                     KLVLVDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2066..4717)
                     /locus_tag="VQ526_001329"
     CDS             complement(2066..4717)
                     /locus_tag="VQ526_001329"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2555046.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4710..5288)
                     /locus_tag="VQ526_001330"
     CDS             complement(4710..5288)
                     /locus_tag="VQ526_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2555047.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5288..5848)
                     /locus_tag="VQ526_001331"
     CDS             complement(5288..5848)
                     /locus_tag="VQ526_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555048.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWGVI"
     gene            complement(5835..6284)
                     /locus_tag="VQ526_001332"
     CDS             complement(5835..6284)
                     /locus_tag="VQ526_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HET2555049.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6697..7092)
                     /locus_tag="VQ526_001333"
     CDS             complement(6697..7092)
                     /locus_tag="VQ526_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2555050.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASHFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            complement(7188..7442)
                     /locus_tag="VQ526_001334"
     CDS             complement(7188..7442)
                     /locus_tag="VQ526_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2555051.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7452..8786)
                     /locus_tag="VQ526_001335"
     CDS             complement(7452..8786)
                     /locus_tag="VQ526_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2555052.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8786..9019)
                     /locus_tag="VQ526_001336"
     CDS             complement(8786..9019)
                     /locus_tag="VQ526_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET2555053.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(9332..9472)
                     /locus_tag="VQ526_001337"
     CDS             complement(9332..9472)
                     /locus_tag="VQ526_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555054.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(9469..9651)
                     /locus_tag="VQ526_001338"
     CDS             complement(9469..9651)
                     /locus_tag="VQ526_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555055.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTARWLIKPEDKK"
     gene            complement(9653..10432)
                     /locus_tag="VQ526_001339"
     CDS             complement(9653..10432)
                     /locus_tag="VQ526_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555056.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            complement(10711..11061)
                     /gene="rbfA"
                     /locus_tag="VQ526_001340"
     CDS             complement(10711..11061)
                     /gene="rbfA"
                     /locus_tag="VQ526_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2555057.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(11312..14104)
                     /gene="infB"
                     /locus_tag="VQ526_001341"
     CDS             complement(11312..14104)
                     /gene="infB"
                     /locus_tag="VQ526_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2555058.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDCYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(14121..14420)
                     /locus_tag="VQ526_001342"
     CDS             complement(14121..14420)
                     /locus_tag="VQ526_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2555059.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(14413..14706)
                     /locus_tag="VQ526_001343"
     CDS             complement(14413..14706)
                     /locus_tag="VQ526_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2555060.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(14728..15864)
                     /gene="nusA"
                     /locus_tag="VQ526_001344"
     CDS             complement(14728..15864)
                     /gene="nusA"
                     /locus_tag="VQ526_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2555061.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(15908..16387)
                     /gene="rimP"
                     /locus_tag="VQ526_001345"
     CDS             complement(15908..16387)
                     /gene="rimP"
                     /locus_tag="VQ526_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2555062.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(16513..17148)
                     /gene="trmB"
                     /locus_tag="VQ526_001346"
     CDS             complement(16513..17148)
                     /gene="trmB"
                     /locus_tag="VQ526_001346"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2555063.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(17145..17939)
                     /locus_tag="VQ526_001347"
     CDS             complement(17145..17939)
                     /locus_tag="VQ526_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2555064.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(18100..18711)
                     /locus_tag="VQ526_001348"
     CDS             complement(18100..18711)
                     /locus_tag="VQ526_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2555065.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
BASE COUNT         5232 a         4298 c         3516 g         5736 t
ORIGIN      
        1 taaaaggact tagccctgtg caatacagaa ctaaatcctt cggataaatt aattgtctaa
       61 ctgtttgggg tcagtacatt ttaacacttg atactatgcg ttttattgtg ggaatgttta
      121 cttcattttt tcctaaaatt caatgtttac tcagtatttg gattatgaaa aatcgaggtc
      181 taaatctaga tacatttttt ctgaagacaa atcattttga ccaccgagca agagattttc
      241 aaaaaaagct gttaaaaact caggacgtcg ctgtaaaatc tttgcattat ctaataccaa
      301 ggcatcacga aaatacttgg aatgttgctg aaatggtata ttatcaatat caaaaccaaa
      361 ctcacgaaga tactgaatca aaaagaccgt tactgtccga gtgtttcctt cgcgaaatgg
      421 atgaatctgc cagattcctg aaataaaatg ctggatttgt ttaaccacat ccgcctgagt
      481 tagtgtcgca tatgcaactt gtttttcctg attaaaatca taatctaagg tcatttgaat
      541 catggagtaa tcagagtaca caacactttc accattcaaa acaggttcat tctttgtgat
      601 attggtctga cgaaattgcc ccaccggaat agagggttca aatatatctt gaaacaactc
      661 cttatgaata gcaagtaagg tcgcaggact aaagctaaag cctcttcgag acaatagttc
      721 tacaatacgt agagaaacca agtctgcctc ttccgtactt gcatcaatag tatggtgata
      781 agccgttgca tcctcataaa cctgctcata agtcagttct ccccgggact gtttctcagc
      841 caaagattcc atatacgctg atggcactag attgtcaact ttctgcagac caaaacctat
      901 ccgccataaa tcacgcttcg cttcataaga caagtttgga ttgtcaatgt tgtaagttga
      961 ttgcatagaa atatcctttc aattgttttt cttcattacc agtaaatcac gaaattggtt
     1021 ggaaactggc ttaaactata aaaagtcctc acaataggtt ctatgatata tatatacttc
     1081 atgtaaataa taaaagagtt ataatcagca ctactgataa attcaatacc gatttcgcca
     1141 tcttaaaact tagtcaaacg aataccgttg atttccagtt cattaagtcg ttgacctgtc
     1201 aagacttctc gttccttcaa ctggaaaagt taagggggaa ctaatacaaa tcctctacgc
     1261 ttctatattc aatgatttca ttttttataa tattcttttt aaactcaaaa tgttttagag
     1321 gattatcaac taatactaac tttggaatat catctaggtt ggtgaccaga gataaaataa
     1381 aatctgattg aaactcttta gctttctcaa attcgtaggc agttaaacgg acacgaccct
     1441 taggttttcg aatcccttta acttcggcaa ggtaggaatg ttcttgaata tctacttgga
     1501 aatcatatcc atcaccataa agacatgcat cggttaattg tccaccttga aatttttctt
     1561 ctttatcaaa atgaaggata aaataattct ctgcctccat ccccgtttcc tgtagacgtt
     1621 tgaatttagt tcgaataatt ggtttttcaa ttatagtaat gcctgtttgc tttccttcac
     1681 tagcaagtaa cattttaaca atttcaacat aactatgaac atcttcattt ccaaacatca
     1741 tatcaattaa atctttacga aaacggtaaa cttcagcttt ctgccaccaa ccttttcgac
     1801 tattgtcaaa ataaggatca aataaatcca ttctattttt tacaactcca gttgtttcag
     1861 caattcccaa agaaaccaca tagcgataaa actccgactt actagaatat tgaaattctt
     1921 ttataaaaag cttggcaaac ttggcaagac cgtagccaat taaatttaac aattcataat
     1981 gcgggtgttt agacatcact ttctccatca atagatagta atttcattat cccaaaaaac
     2041 acggcattac accatgtttc tgtgtttatt tcaccaactt cttcatctca tcaatacgtg
     2101 ctacggtcgc atcatacttg gcttggtagt cagcttgttt gtcgcgttct ttttggacga
     2161 cttctggttt ggcgttggct acgaagcgtt cgttagagag cttcttaccg accatgtcca
     2221 gttctttttg ccatttagca agttccttgt caagacgagc cagttcttct tcgacattga
     2281 ggaggtcagc caatggcagg tagatttctg ctcctgtaat gacgcttgac atagccagtt
     2341 caggtgcagg gatggttgat gcgatttcca agtgttctgg atttgtaaag cgtttgatat
     2401 agttgacatt gctgttaaag aaggcttcca agtcgctatc gcttgtctta acaaggatgg
     2461 tgataggctt gcttggtgct acgtttactt ccgcacgcgc attacgaacg gcacggatca
     2521 agtctttgag actttctaca ccagtatgag ccgcaaggtc ttcaaaggct agattaacag
     2581 ttgggtatgc agctgtcacg atagaacctt ctgagatttg tccaaagatt tcctctgtca
     2641 cgaatggcat gattgggtga aggagacgaa ggatcttgtc cagagtgtaa aggagaacag
     2701 atcgagtaat gaccttatcg tcttcattgt cgctgtaaag gacttccttg gtcaattcaa
     2761 cataccagtt ggcaaattct tcccaaatga agttgtaaag gatatgacca gccacaccaa
     2821 actcgaactt atcaaagttt tcagtaactt ttgcaatggt ttcgttgaga ttgtggagaa
     2881 tccagcggtc cgtcacatta ccagcctcac ctgttgcaac ttttgtgaca ttgtcatgcg
     2941 ccacatccag cgtcaaacct tcattgttca tgaggatata gcgagaaatg ttccaaattt
     3001 tgttaataaa gttccatgaa gcatccattt tctcgtaaga gaaacgaacg tcttgaccag
     3061 gagctgagcc gtttgacaag aaccaacgaa gactatctgt accatattta tcaataacat
     3121 ccattgggtc aatgccattt ccaagagatt tagacatctt acgtccttct tcatcacgaa
     3181 ttagaccatg gataaggacg ttttggaatg gttgacggcc tgtgaattcc aatgactgga
     3241 agatcatacg agacacccag aagaagatga tgtcgtaacc tgttaccaag gttgaagttg
     3301 ggaaataacg tttaaagtct tctgagtcga cttcaggcca gcccatggtt gaaaatggcc
     3361 agagggcaga actgaaccaa gtatccaaga cgtcttcgtc ctgagtccat ccgtcacctt
     3421 ctggagcttc ttcgccgaca tacatttcac catcagcatt gtaccaggca gggatttggt
     3481 gaccccacca aagctgacga gagataaccc agtcgtggac attttccatc cattgaagga
     3541 aggtatcgtt gaaacgaggt gggtagaatt cgaccttgtc ctctgtgtct tggttggcaa
     3601 tggcgttctt agccaattgg tccatcttga cgaaccattg agtagacaag cgtggctcaa
     3661 ctacgacacc tgtccgttct gagtgaccga ctgaatggac acgtttttcg attttgacaa
     3721 gggcaccgat ttcttccaac ttagcaacga ctgccttacg agcttcaaaa cgatccatgc
     3781 ctgaaaattc aaaggcaagc tcattcatag ttccgtcgtc gttcatgacg ttgacttgtg
     3841 gcaagttatg acgttggcca accaagaagt catttggatc gtgggcaggt gtgattttca
     3901 cgacaccagt accaaactca ggatctgcgt gctcatctcc aacgattggg atgagtttat
     3961 tagcgattgg aaggatgacg tttttaccaa tcaagtcctt gtagcgcggg tcttctggat
     4021 taaccgcaac cgcaacgtcc ccaaacatag tctcaggacg agttgtagca acttcaaggg
     4081 cgcgtgaacc atcttccagc atgtagttca tgtggtagaa ggcaccttcg acatccttgt
     4141 gaatcacctc aatatcagaa agggctgtgc gagctgctgg gtcccagttg atgataaact
     4201 caccacggta gatccagcct ttcttgtaaa ggtccacaaa gaccttacga acagcttttg
     4261 acaaaccttc gtcaagagtg aaacgctcac gagagtagtc tacagagagc cccatcttgc
     4321 cccattgttc cttgatagta gtggcatatt cgtctttcca ttcccagact ttcgtcaaga
     4381 aagactcacg accaaggtca tagcgggaaa tgccctcacc acgcaagcgc tcctctacct
     4441 tagcctgagt ggcaatccct gcgtggtcca tcccaggaag ccaaagggta tcaaaacctt
     4501 gcatgcgttt ttgacggatg ataatatctt gcaaagttgt atcccaagcg tgaccaaggt
     4561 gaagtttacc tgtaacgttt ggtggtggaa taacgattga ataaggctta gccttttgat
     4621 cgcctgaagg cttgaaaaca tcagcatcaa gccatttttg gtaacgacca gcctcaacct
     4681 cggctggatt gtatttagat gaaagttctt tagacatgtg tgtgtccttt ctctattttg
     4741 tttattttat tttgaatttg cttagcagct tcttctgcag acaaattcgt attatttatt
     4801 ttaaagtagt ggtgcaactc attcggttga tattgggaat ttaattgaag tgtttcagcg
     4861 gtctctaaaa tttctctttc agatacctca atatgtcgtt ttaagggttt gtgctttaat
     4921 cgattctccg ttcgatttcg acgtatgcac tcttcaagac ttgtttccaa ctcaacaaaa
     4981 agaatctctt gatgataatc atccaacaaa ttctgaattt gctttaaata tatcagatga
     5041 tactgatttg aaaaatcaat tacatctgtt aaaattactg atcgctgatt tcttgcactt
     5101 gctccaagga atgaaaaagt gattccacga acaaattccc acatttcctc tgtatagtcc
     5161 tgatagattt ctagtgcaaa atcaatggct tgatggttat aaaatagggt agcatccgtc
     5221 agtcgagata attcttgacc aatggtcatt tttcctgatg ctggagcacc aatgatgaaa
     5281 agatgcatca aatcaccccc cactcactct tcagcaaggc atatctcaaa tcatcacagc
     5341 agttgccttg agcatcttta cggtctctta tgcgagcttc gagggtaaag ccaagctttt
     5401 ccgcgactcg ttgactttga aggttatatc caaagcaagt tagttcaatc ttgtgaagac
     5461 ccaaatcttt aaaggctaag tcaatcaagg cacgcgcagc ttctggcaca taacctcgcc
     5521 cccaatagtc tgagtgtaag gtatagccga tttccatcac atcatcttca tggcgatggt
     5581 tgaaatagat agagccaacg atcttatccg ttcctttgac gacaatcccg tagcctgctg
     5641 ggagattttc cttttgatta cgctccggaa gaatgtgctc cagataataa atctcatctt
     5701 ccaaggtctt gactggagga aaacctgctg gataggcgac ctctggcaaa ctagcatagt
     5761 caaagatatc ctcagcatcc gccacagtac ggactcgtaa aacgagacgt tctgtttcga
     5821 ttttatctgg cagctcagtt cttgccatcc ttcttcctcg cttttttgat gaaactgccc
     5881 ttcatatcta cacgcttgtc cagatagcga taaacgcgct gatatccatc tcccatgaaa
     5941 taggttgggg caaacagttg atttttaaaa tgtccctttt catccaggag ttctggggca
     6001 acaagtcgct caagaatctt ggcaaagatg tggcaaatac cgtcttcctc aacaatccta
     6061 tctacccgac aatctaaaac aagtggacag gcgtctaaaa taggagtctg agttcgttca
     6121 gaaatttcat aatgcactcc caaacgttcc aatttctcct gatgactgat aaaaccagcc
     6181 tgctccatcg caagcataga agtttcatca gaaatattca cagtaaattt ttgatactgt
     6241 ttgatctgct ctgcggcatt ctctctcgca acgactccaa tcacaaccca atctcctaga
     6301 ctataagagg aactacaggt cgtgatgtta tagccaaaat tctaatcttg atatcctaaa
     6361 ataaaaacag gaaaaccata atatagttta cttgtgttaa aagattgctt cataacaacc
     6421 ccctttgact aagacgtaaa agaaaagccc tgccatctac atgacaggga cgaatgtgtt
     6481 tatccgcggt accacccaat ttcgggcagt tgcccgcaac tctcgtcttt aaagcaaaaa
     6541 gacactttta tttcaatttt tcatccatta gcaaccagtt ttgacacatt tgtggacttc
     6601 tcagcgccgc cactttctgt aaaatgtggg attcaaaaca cctctaatgg ctctattgta
     6661 gcaagatttt ttcggaaatg caaggctcaa gaaaaattac ttgaacttga tgccattttc
     6721 tttcaatttc ttgatggtag ctgggccgat tcctttcaag tcaaggagtt ctttttcagt
     6781 ccagtttttg aaatgtgaag cagacttgat tccttcatca tagaaagtct tggcacgatc
     6841 aagtggaagt ccacccaaat ttgctgcaaa atcttctaca gaattagcta ctttttgagc
     6901 ttgctctttg gtagcttcta ctgcttgttc aactttttta gaaacagcct taccagctgc
     6961 gcttgctgac tgttctgcac gcttcacgac tttttttgtc tcatctacaa ccttagtcac
     7021 agtacttgaa aaggcacctg cgcgacggag gccatttcgt aattgttttt tacgattgag
     7081 tttctttgac atgataaaat cctttcaata aaaaatccct gctctgacaa ggatttaaca
     7141 taaacattct atcaagattt tagtgaaaaa tcaagaatct ttctcgttta ataatttcgc
     7201 tcaccaaaca agccttctgg caaatcatgg aatcccttgc ctactttgaa gttttcaaac
     7261 ttaccaagca agaactgata agcatccaag cgactctcgt agcgaatcat ggcatctttg
     7321 gcacgctcgt cctggtcttc ttcgtaaact ttttgacttt ccttgtgcgc catatcgtta
     7381 atcatgattt gggtattgac ccaagcttca aattccttca taaattcttg ttcgtaactc
     7441 attttttctc cttattctaa tccacgaaaa tagtccgtat caatctcacg gtagggctgg
     7501 atatcctgaa catactccaa cactccttgg aattctccgt cttcatcgtg tactgcagca
     7561 taggtgatgt ggacaaactt acctcgcgac tcagacttga accacatttc atacttgtcc
     7621 ttggtcccct cacgaagtcc cttcatgata gccttgacct tgtccaaata cttaggcgga
     7681 tgacagagtt cgacattgcg cccgacttgg gacggcgtcc gtttgaaaat catctcatca
     7741 gctggcgtat tgtcattgta atactggaaa atatcttctt tattgacaaa ggtaatctcc
     7801 atagggagat gattgaggat gagattggcc tgctcgactg aaagatagcc attaccaaaa
     7861 gcctgttgac tatggcggtc cagcacagct tccttttcct taggggtaaa ggtaatggta
     7921 aaatggcctt ctggcgtatc tatgacttgt tgaacttgac cttctgccgt atctagttgt
     7981 acaggctcct ctgcaatctt ttcctcaata aagatctgtc gttctggcac ccatttctct
     8041 gacggacgga tgatggcata gccataggca tcactctcct ccgcaatctg aagccagtca
     8101 tcctgagtaa aagactcaag gagaatcatg agcaggatgg actcttcctt gaaaatcata
     8161 ctttcaaact ctgtcgcaaa agcttcaaaa gcttccttta cactgctaat tgacacttct
     8221 ggtagtgact tggctgtcgt tagagctgtt tgaaagagtt ccctaatctg atcatccact
     8281 ccccacataa ctttgggagg tgaatcgtgt ccatagcgct ccatgatagg aaagaagagt
     8341 tcttccttac gttggtaatg gatgtcaaat tgacccacaa gtcccatctg acgcaccaaa
     8401 cccttacgca tctccgccag catttcctcg tcttccatag actcataggt atctaacaat
     8461 ctacgaatgc gaatcaaggc cgcacggaga gccagatttt cttctttgaa gacacgaact
     8521 gggtgacctg gatgctcagt atctgaaact tcgacacctt tgatagcatt tttaaaaaga
     8581 ttggcatgga catcacagag ttccataaca tcttcaaaag tgacgcccga atccgagttc
     8641 atcagctcgt gctccataag ggaaatctca atggctgaga cacccgcaaa ggtcgcatca
     8701 aaacgctcct gaaccgactc aggagaggcg ccattatgca attctaataa aatatcccgt
     8761 aggacatgaa tccgttcatc tgccattagt ctaatccaat cacttcgtag ccattcgctt
     8821 ccagtgtgcg tacaatcttg tccataggag ttcctgctag cttagaaccc tgtttaagtg
     8881 atactttacg accaactgta ttgcgcatta agggattggc aaggggttta aaacccaact
     8941 ccactagaat ttccaagact tctggatgct tgtccaccac ttctgcaaca ggaattgaca
     9001 catcgatgat attgtccatg acgacctcca ttatatgttc taatactcaa tgaaaatcaa
     9061 agagcaaact aggaagctag ccgcaggttg ctcaaaacag tgctttgaga ttgtagatag
     9121 aactgacgaa gtcagctcaa aacaccgttt tgaggttgcg gatagaactg acgaagtcag
     9181 taacatatat acagcaaggt gaagctgacg tggtttgaag agattttcga agagtataaa
     9241 atgattttac tgcttatatt ataccatatg gaaagagaat aaaaaactag cagtggagtt
     9301 cctgctagtt ctctgcgact tggattacca tctagataag tttaaagaac caatccaata
     9361 tcttgtaaaa cagcaattgg actgcaaaag agatgatcat ccaaaagaat ttcacaatcc
     9421 caataatcgg tttgataaaa ataatggcaa gaagtcccca gaaaaatttc attttttatc
     9481 ctctggctta atgagccacc gagccgtatc cattaacttg tcggcaataa aaagtttccc
     9541 cagaccgaca acggcattat catctttctt tatcgttttc attactttta caccttgcac
     9601 ggtcaaaaag taattaccat aaattttagc aaaagctttg ataggattca tattactctc
     9661 ctttgatgat ttcagcctct tttcggattg tttcaacatc ttcttgatat tgaacttcac
     9721 tatagttgac tagcttggat aattctttct gcaagctttc ccccattggt ttcatagttg
     9781 caaaggttaa accacctgaa atgataccac ccaagatagg aataaatttc cccattcctt
     9841 tggccagccc tcccttggta agattcacac caaatattct taagactttc ttcaaaatag
     9901 gataccaaag cgtctttgtt aaagctttat taggtactgt tttcattacc tgtttggcaa
     9961 ttgttatacc accagcacgt agcaaagcag cagttccatt cacccccaac atgacgccta
    10021 gataaagcaa aagggtattt tgagcatctt cactcaactc atcgcgtgaa gcccaaagat
    10081 cctcataacc ataaatataa cctaattctt gggccaattt cagagagaaa ccataaaatt
    10141 gagccacatc agctggaatg gtaattgcca tagcaagccc tccaggtaat cctgccaaaa
    10201 cagaagtccc actcgctaat aaaacattat ccctaataca ggcattagcc actcgatcta
    10261 agatttcttg agataagaga gacgttggac cttgttccaa taaggtagga atgtcctgtg
    10321 gacccaattc tttggaaaac ttatccacta aaaatttcga acgatcaacc ttaacaacag
    10381 gtagtttcac cacttgttgc aatacttgca tagctaagtc ttgttcagcc atatacaaac
    10441 tccttatttc ttttttgcac cttatcatat tatatttcct acaaaaaaag cattttctaa
    10501 ttagataaca ttaaaaaata cagaacccct tttttatagt aaaatgaaac aagaacagga
    10561 caaatcgatc aggacagtca aatcgatttc taacaatatt ttagaagtag aggcgtacta
    10621 ttctagtttc aaactattat aaaagaatat ttttccattt caaattcaac ctattttcct
    10681 ccaccaaaaa agaggggcaa cccctcttct ttagttctta tccagattgc gtagcatctc
    10741 gtcaatcttg tttccatact cgatggactc gtctttgacg aaggtcaaat ctgggatttt
    10801 gtacaatttc aaattgcgac caagttcacg tttgatggta ccagttgctt tttcaagccc
    10861 gatttgggct ttttggttat ccgaagcaag gttactcaaa atggtgtaat aaaccttggc
    10921 aacagacaag tcacccagca tctgaacatc tgtgatggtc acaccttgga cacgtggatc
    10981 acggactttc ttttgcaaaa tctcattgac ttcacgcttg atttccatgc ccacacgatc
    11041 tgtacggaaa tgatttgcca tgatattcct ttctctactt tgaaccaaaa gctaggccag
    11101 cagacctagc tattttaaac ttcttgattt tcagtttaaa ttgaaacgaa gttcaatttg
    11161 aagtcatctg cttctttcgc cgtggtgaaa gtgatggaac tgatttatca gtcccatcac
    11221 aggggatttt tgagacccta ggctcaaaaa taagcaatga aaccatcggt ttgcttgcgt
    11281 ccctcaccac ctaagaaagg agcaaaaaat cttatctctt gatttcttcc atgacatacg
    11341 cctcaatcac atcatccatc ttaatatcat tgtagcagtc gatcatcaat ccaccttcac
    11401 ggccgtttgt aacttcctta acgtcgtctt tatagtgttt caagcttgcg agttcaccat
    11461 catagataac gacaccatca cggataacac ggactttaga gtcacgggca accttaccgt
    11521 tgataaccat aaatccaccg atagttccca ctttagacac cttgaaggtt tcacggataa
    11581 ccgcttcacc aataactttt tcttcaaatt ctggatcaag catccctttc atagcttctt
    11641 ccatctcttc gataaccttg tagataatgc tgtgaagacg gatttccaca tcgtcagctt
    11701 ctgcttgttg acgagcttgt ggtgtagggc gtacgttgaa accaacgata aaggcatttg
    11761 aagcttcggc aagggtcacg tctgattcgt tgatagcacc gaccgctgag tggacgatag
    11821 tcactttgac accttccacg tcaatctttt gaagtgaggc agaaagggct tcaacagaac
    11881 cttgtacatc agccttgatg ataacattaa cagatttgag ttccccagct ttaagggtat
    11941 caaagaggtt ttcaaggcta acacgttggg tagcttgacg ttgtttcatg agggcacgtt
    12001 tggcacgctc ttcacctgct gcacgcgcag atttttcatc ctcgtaaacg gcaaagtggt
    12061 cacccgccat cggtgcttcg ttcaaacctg tgatagagac tggtgttgat ggtccagcaa
    12121 ctttaacacg acgaccaagg tcgttggtca tagcacggac acgaccgaag gtatttccga
    12181 caacgattgg gtcttgaaca ttcaaggtac cttgttgtac aagaagggtt gcgaccgcac
    12241 cttttccttt atccaagcgc gcttcgataa ccgtaccgat tgcacgcact gttgggtctg
    12301 ccttgagttc ttggatttca gctacaagaa ggactgtttc caacaattct tcgatatttt
    12361 ggttgaattt agctgagatt tcaacaaatt cagaatctcc accccaagcg gttgacatca
    12421 caccatgctc tgccaattca ccgataacgc gttctgggtt agcacctggt ttatcaatct
    12481 tgttaatggc tacgatgatt ggaacgttag ctgcttttga gtggttgatg gcttcaatag
    12541 tctgaggcat aaccccgtca tctgccgcta cgaccaagat cgtaatatcg gtaacagaag
    12601 caccacgcgc acgcattgat gtaaaggccg cgtgtcctgg tgtatcaagg aaggtaatct
    12661 tcttaccatt ttccacgatt tggtaggcac cgatatgctg agtaatacca cctgcttcac
    12721 ctgtcgcaac acgtgagtta cgaagagtat ccaaaagggt tgttttaccg tggtcaacgt
    12781 gtcccatgat agtaacaact ggtggacgct caaccaattc atcttcattg agataaccat
    12841 cttcgacaaa gaaacgttcg atgtcagcat tatccacttc aaccttttgt ttggcttcga
    12901 taccgtaatc caccatgagg agttcaattg tttccccatc caaggattgg ttttgtgtgg
    12961 ccatgacacc catcatgaaa agtttcttaa cgatttcagc tggttcacgt ttgatacgtt
    13021 ttgcgatttc cgcaacggtc ataccatctg tatattcaaa ttctgttggc aattcatgga
    13081 atttacgctc cgtaacaggt tttggagtct gattacggtt gttcttgtta ttgccttttt
    13141 tgttcttttt gttgttattc cagttactat tcttttgatt tctcacttga ttttgactac
    13201 ttcgattctt ttgttgtttt ctaggaccat cttcttcatg atcataatcg tcacgatttt
    13261 tgtctggtcg agcttgtttt ttacgacgtg tatccactgc aggttctttt ttctcaggga
    13321 cgacttcaac cactgcttga acttgctgtg cttgttcagc taacttagcc gcttcttcaa
    13381 agatttcctc tggttccttg cgtttgttag cttgagccaa ggcttcttta gcagcctgat
    13441 actgcttgaa gcgttcctca cttgaacgag cgtactctgc attttgctct gcttttaggg
    13501 ctgctgcacg ggctttaaag tcaatacgtg gagccacttg atttgaacgt ttgtcctctt
    13561 gctgacggcg ctcatttcta cgtttttgct gaccttgctg cttcttagct tggtcattaa
    13621 agcgacgatt gtcgcggttg ctttgacctt gttttccacc attacggccg tcgtttttct
    13681 gacggtttcc gttttgttgt tggtcacggt tattgccctt attttgcttg cgtcgttctg
    13741 cctgctcttt agcacgggct tcacgctcag ccttgaaatt acggctttgc ggtttcgctg
    13801 ctactttttc tgttttcgga gcaactggct cagccggttt tgcctcttcc ttagctacag
    13861 ctggtttagc tggctcagat ttttcggctt tcttttctgc acttactttt ggtgctgcag
    13921 gttttgcttc tactttcgga gcagctgcag gcttaaagct ggcagcaatt tttgcagcga
    13981 cagcttcttc cacacttgat gagtggcttt tcacatccaa gcccaactct tttgcacgcg
    14041 ctacaacttc tttactttct tttccaagtt cttttgcgat ttcgtacaat cttttcttag
    14101 acaaatcatg tcctcctctt ctattccata agagacctca ttttctttgt aaatccagca
    14161 tctgttacag ccaaaacctt tctcgatttc ccgactgcta tgcttaattc cagtgttgaa
    14221 aacacggtta caatttctac ttgataataa tgacttttat cttgaatctt cttggtcaga
    14281 ttgggtccag catcatgagc tagaaagacc aacttggcct tgccgtcttg aatggccttg
    14341 accaccaatt cttcacccga tatgatgcgc cctgctcgct gagcaagccc caagagatta
    14401 cttatctttt gcttattcaa gtcccaactc tcttcttttc actttgtgat ccacataagc
    14461 gatcaactcg tcataaaagc tttcttccac ttccatgcta aaactgcggt taaagacctt
    14521 cttctttttc gcctctaggg cttctgcatt gtctagtttg atataagcgc cgcggccatt
    14581 ggccttgccc gtaggatcaa taaagacttg tccttccttg ttcttgacaa tgcggagcaa
    14641 atcacgctta tcaatcactt cgttagacac aacagacttg cgcaaaggga tttttcttgt
    14701 tttcatcttt ccctcctcta gcagctttta ttcttctaca gtatcgtttt ctacttccaa
    14761 ctctactgaa gcagcgtctt ccatggcttc aaattcgcta gcagacttga tatcgatacg
    14821 gtaaccagtc aagtgagccg ccaagcgcac gttttgtcca cgacgaccaa tggcaagaga
    14881 aagcttgtta tctggaacaa ccaccaaggc acgtttgctg tcgttttcat caaagataac
    14941 ttggtcaacc tcagcaggag cgatggcatt gtagataaat tcagctggat ctgctaccca
    15001 ctcgataaca tcgatatttt cttcgattgg taccatgcgg tcatttttag catcgtaacg
    15061 agctgggtgg aatttgctag taatcttctt gatattagca ccaccacgtc caacgattgt
    15121 accgatagca tccacgtttg gattgtggct acgaacagca accttcgtac ggtcacctgc
    15181 ttcacgagcc acgctcatga tttcaacagt tccatcataa acttctggaa tttcttgctc
    15241 catcaaacgt ttgatcattt ctggatggct acggctaaca aagacgttca caccacgagg
    15301 gttgtcttca accttgtaaa cataaacttc gatacgatca tgagaagcaa aaacttctcc
    15361 aggaatttgg tcttgttttg acaattgggc ttcgatgcta ccaaggttga catagataaa
    15421 gcggttgtca aagcgttcta ctgtaccaga catgatttct tgctcatgtt ctttgtaagt
    15481 attgtaagtg atggcacgtg tttgcttgcg cattttttcc atgatggttt gtttggcaga
    15541 ttgggctgct acacgaccaa actcagctgg tgcttcttca aacttgattt tgtctccaag
    15601 ttcataagct gaattaatgg caagagcatc tttcaagctg atttccaaac ggctatcaaa
    15661 tacttcatca acaacttcac ggacagtata aactgtaaag tcacctgttt tttcgttgaa
    15721 gtcaatagct acgctgtctg actgaccata gcgtctgcga taagcggaac gaagcgactc
    15781 tactactgcg tcgatgatat cttctttttt gattcccttg tcttcttcca aaatgcggaa
    15841 ggcctctagc atttctttac tcatgttttc ttcacttttg aatcctcaaa agctatcctt
    15901 tctttttcta taatttaact gctaaacgtg cttttgatac taaactgtat ggaatttgga
    15961 cggttttctt acgcgtcttg tccatatatt ccatagtcaa ctcgtcctct tcgaaggcca
    16021 acaaggttcc ttcaaagacc ttttgcttat cgatggcttg gtagagcccg acatggatgt
    16081 attttccaac cgctccagcg acggcatcct tggttttcaa aggacgttcc aaacctggac
    16141 tggtaatttc taggaaatat tgttctggga agggatctgg cttgatggtg tctaggacag
    16201 gactgatcat ttctgtcaag tctgccgtgt cgttcaaggt aattccttcg ggtttatcta
    16261 caaaaatact gagaatcatg tcactgccaa tctttccata ctcgatatcc acgagttcaa
    16321 aaggagcttc tatgacaggt tctacaactt ctctgactaa ttctacgatt gttgcgattg
    16381 cgtccacctc ctcataagca agaggcgaag atatttcccc gcctcacttt tcatattctt
    16441 actatcagta tagcacgttt gccaacttat gtaaagcaaa agcacttgta cagcctaatt
    16501 ttaggctatc tcttaaaatt ctgcctcaac tcggtagata acttgcccct tgtttgagaa
    16561 tttttgctcg tattctgtca tgacattgcc ttcaaaatca ctggcatgca aatctaacca
    16621 gacaccattg agtttcatgc catattgaga aaagctcact aaactgtact caaacaagcc
    16681 acggttatcc gtcttgaaat gaatttctcc attttcaggc aagatacgtt tgaaggtatc
    16741 caagaaggtc ttgtaggtca aacgacgctt ttcatggcgt tttttcggcc atggatctga
    16801 aaagttcaga tacaagcgat caatctcacc gtcttcaaag tagtcagtta agtcagaacc
    16861 atctacccac aagagcttga tgttaggcac tccaacttca agcaccttgt ccaaagcgta
    16921 gctcaaaaca gacttttgaa tatcaatccc gatatagttg atgtcagggt tttgcttggc
    16981 catacctgaa acaaaggcac cctttccact tccaacttcc acatgaatgg gattatcatt
    17041 gccaaacaag tcccgccatt ttgccttggc ttccaaggga ttgaggacca catactgggg
    17101 atttgcctct agtaattctg ttgccccttt acgatttcta actctcatct tctctttcca
    17161 tacttgtctc ggaaatgacg caaaccatga atctcccgat tgacattttc taaatcttgg
    17221 ttcatataat acttggaaat ctgactcaaa taagacaatt gaccatacca atacaattta
    17281 cttaataccg tttgattgta cttgtaaccg tagtaggtca accattcctt ccactgatgt
    17341 tctgaaatat aatggcagag catatgggcc acatcaaaca tgcgatcggt caagcgaacc
    17401 gaatcccaat ctactaaata aatcaagcca ctatctgtct caatccaatt actatgtcgt
    17461 acatctccat ggacaatggt cgcatggtcc tctctaaatc ctggaatagt ctgacgtaaa
    17521 tcagccatca cttcactgat aaaatgattt ttacgcaaag catctggagc cgtttcctgc
    17581 caagactgta gtaaatctac aggtgtttcc atggcatagc ccaaacgact caattgtgtc
    17641 atcaacggac gtgagcgatg aagacgggtt aaaatattga cgatttgctt acgattcata
    17701 tcataggggg tcaatatctt gcctgtcaac cattcttgag cacacatatc acgcccatct
    17761 gccaaacgac gactccataa taattgtgga gcaatttgtt ctctagctag accaggtagg
    17821 attggagagg tgttcatttt tacaaagatg cgcttcccgt caggatagct acccatataa
    17881 gccttgccac ttttcccagg tatgggagtc agtgttagct cattatcacc caaatccatt
    17941 tctttcctcc gtataaagtt tccttactat tctactagtt tttggcctat tcgtcaacca
    18001 ctctaacccg attctcaaag aaaagcctct ggattggctt gggtatcatt atagcttgaa
    18061 aaaggtaagc accatcttct gcttaccttt tcataatttt tataaataat ataggagtgg
    18121 ccaactgttg taaaacatgt ggactagagt agaaacccat aggttttgac tctttttata
    18181 agcaaagccc agcaacaaac ccccaagtag ataaaaaata aacgaaggga cattagaagg
    18241 tccatgcatg aaagaaaaga gagaggaagt tagcacaagc cctaaccaag aattgtcaaa
    18301 aaccgcacct tgaagaagtc ctctgaaaat gagttcctcc tggatggggc caaaaactga
    18361 ggtagtcaaa atcaaagaca gggaaattcc tctgctgact tccgccaagt gttggacccc
    18421 tgagccagaa gaaacagcga agaaatgaat ataaaccagc gtctcaagta cacttagaag
    18481 aaagattgca atgaaaagca aaagttgttt cttgcttaac atcccaaaag aaatcatttc
    18541 aaactttcta gcataagcaa cactcagcga ggttacaaga agacttacaa gaatcaatgc
    18601 agattcattt tgaaaaaaac ccccatggtt aatcgtataa ggaactgtaa tgacaattat
    18661 tagtactaaa aatccaaaac acaaggcatg aaatttatca aaaaactcca taaaactatc
    18721 ctcctcacca aacagacttc ctacgcgtca ttctttagtt ctagcctttc caaaacaaac
    18781 ca
//
LOCUS       DAVBDG010000038        18669 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBDG010000038 DAVBDG010000000
VERSION     DAVBDG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.038"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..852)
                     /gene="lytB"
                     /locus_tag="VQ526_001349"
     CDS             complement(<1..852)
                     /gene="lytB"
                     /locus_tag="VQ526_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET2555066.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYTENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYF"
     gene            complement(894..1880)
                     /locus_tag="VQ526_001350"
     CDS             complement(894..1880)
                     /locus_tag="VQ526_001350"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2555067.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1843..2643)
                     /locus_tag="VQ526_001351"
     CDS             complement(1843..2643)
                     /locus_tag="VQ526_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2555068.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2893..3273)
                     /locus_tag="VQ526_001352"
     CDS             complement(2893..3273)
                     /locus_tag="VQ526_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2555069.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3331..3690)
                     /gene="rplT"
                     /locus_tag="VQ526_001353"
     CDS             complement(3331..3690)
                     /gene="rplT"
                     /locus_tag="VQ526_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2555070.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3742..3942)
                     /gene="rpmI"
                     /locus_tag="VQ526_001354"
     CDS             complement(3742..3942)
                     /gene="rpmI"
                     /locus_tag="VQ526_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2555071.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(3975..4505)
                     /gene="infC"
                     /locus_tag="VQ526_001355"
     CDS             complement(3975..4505)
                     /gene="infC"
                     /locus_tag="VQ526_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2555072.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4521..4652)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4818..6002)
                     /locus_tag="VQ526_001356"
     CDS             complement(4818..6002)
                     /locus_tag="VQ526_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555073.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFN
                     RS"
     gene            complement(6006..6593)
                     /locus_tag="VQ526_001357"
     CDS             complement(6006..6593)
                     /locus_tag="VQ526_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555074.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            complement(6626..6814)
                     /locus_tag="VQ526_001358"
     CDS             complement(6626..6814)
                     /locus_tag="VQ526_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555075.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYGRYEVINYYSGGY"
     gene            complement(6993..9233)
                     /locus_tag="VQ526_001359"
     CDS             complement(6993..9233)
                     /locus_tag="VQ526_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2555076.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSFQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQQGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGVDSW
                     KIESVR"
     gene            complement(9217..9867)
                     /locus_tag="VQ526_001360"
     CDS             complement(9217..9867)
                     /locus_tag="VQ526_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2555077.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            complement(9935..10504)
                     /locus_tag="VQ526_001361"
     CDS             complement(9935..10504)
                     /locus_tag="VQ526_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555078.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            10681..11792
                     /gene="ald"
                     /locus_tag="VQ526_001362"
                     /pseudo
     CDS             10681..11792
                     /gene="ald"
                     /locus_tag="VQ526_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            complement(12159..12419)
                     /locus_tag="VQ526_001363"
                     /pseudo
     CDS             complement(12159..12419)
                     /locus_tag="VQ526_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(12422..12922)
                     /locus_tag="VQ526_001364"
     CDS             complement(12422..12922)
                     /locus_tag="VQ526_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555079.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            complement(13102..14070)
                     /locus_tag="VQ526_001365"
     CDS             complement(13102..14070)
                     /locus_tag="VQ526_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2555080.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQDKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(14156..14371)
                     /locus_tag="VQ526_001366"
     CDS             complement(14156..14371)
                     /locus_tag="VQ526_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2555081.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(14380..15234)
                     /gene="cvfB"
                     /locus_tag="VQ526_001367"
     CDS             complement(14380..15234)
                     /gene="cvfB"
                     /locus_tag="VQ526_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2555082.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(15294..15851)
                     /gene="frr"
                     /locus_tag="VQ526_001368"
     CDS             complement(15294..15851)
                     /gene="frr"
                     /locus_tag="VQ526_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2555083.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(15860..16597)
                     /locus_tag="VQ526_001369"
     CDS             complement(15860..16597)
                     /locus_tag="VQ526_001369"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2555084.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(16681..18015)
                     /gene="trmFO"
                     /locus_tag="VQ526_001370"
     CDS             complement(16681..18015)
                     /gene="trmFO"
                     /locus_tag="VQ526_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2555085.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(18359..18613)
                     /locus_tag="VQ526_001371"
     CDS             complement(18359..18613)
                     /locus_tag="VQ526_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2555086.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGEHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
BASE COUNT         5355 a         4249 c         3453 g         5612 t
ORIGIN      
        1 gaagtagtac caggcttgac tatgagaatc gtagacccat tctttttcag ctattttccc
       61 atcaaatttg agataaaacc aagattcctt atcccaaatc cattcattgg ctgccatgta
      121 gccaccagat tttagataat aatagtgacc attctcgaaa atccattctt tatcagcata
      181 gtttccattt tcttttatgt aaaaccaaga ttggtattgt ttgtcaaaaa gccaaccttg
      241 ctgtactttg gcaccactag cattcacata agcttgatta atccactgac ttgttagtag
      301 ataaccaccg gatttgaaat aatagtcctt ccctttaatt tggagccatt ctttctctgc
      361 gtgctgtcca tctgctttga tataaaacca agcatcgtat tgagaatcat agacccagtc
      421 ttctattact ttggcacctg ttgcaccaac ataggaagtt cctacccaag catttctttt
      481 catctttcca tcttggtcaa gataataaaa ggctcccttg tcttctaccc attctgattt
      541 ggccatatag ccaccagatt tgaggtaaaa atagtcgtca ccttgcttta gccattcatt
      601 ttcagtatag ttagcatctg cttttatata gaaccaagat tgataatgag tatcaaaaac
      661 ccactcattc gctgcttgac tgccatcttc tttcagatac tgttttcctt gccaagtacc
      721 atcactagcc attgcaccct ctggactagc taagaaaaat agcagagcta aaaaaataaa
      781 tcttactttc ttcattaacc ttcttcctct gttcttattt attttattgt accaaaattc
      841 tcctaaattc atattacaaa ctaataaaaa atcaagaaca gattgattgc agtttacctc
      901 agagactctt ttacttcctg acggagttct tccagactgc taataccgta tttatccatg
      961 acttttggta aattttcgat gatgtcaggg caggcataag gattggtaaa gttagctgtt
     1021 ccaactccga tagcagatgc cccagccaga tacatttcta gggcagcttc agccgaatcc
     1081 actcccccca ttccaatgat aggcaggtct gttgtttggg ctacttggcg gatgagtttg
     1141 agggctactg gaaagactgc tggacctgac attccacctg ttccattggc caagattggt
     1201 tttctggttt tgaggtcaaa gcgcattcca accagagtat tgatcatggt caagccactt
     1261 gctcccgcat cttctgcagc ttttgcgaca gtaacgatat cggtcacact cggggttaat
     1321 ttgacataaa ctggcacttc tgaggcttcc acagctgctt tcaccacatc ataagccaaa
     1381 tctggatctt gaccaatcaa aagtccatga ttacagtggt caacattggg acaagaaata
     1441 ttgagctcga tagcttttac attagttgcc ttggaaatcc catgagaaac agctgcatac
     1501 tcttgttttg aaaaaccagc tacattggca ataataggaa gatttggata ttctctttcc
     1561 agccaaggta gcttttcagc caaaacaacc tctaaaccag gattttgcaa gccaattgca
     1621 ttgagcatac cagcaggcgt ctctgctact cttggagttg ggttcccaaa acgtggttca
     1681 agggttgtcg ccttgatcat aatagaacct aaaaggttta aatcatagta cttggcatac
     1741 tcttgtccaa agccaaaaca acctgatgct ggaataatcg gatttttcaa atccaaacca
     1801 ggtagagaca cttgtaatcg atttgtagtc ataattttct ccttataata caactgttcc
     1861 tgtgcggaaa acaggaccat cttcacagac gcgttggctg accgtctcgc tttctggtac
     1921 ttttagaaca caggcatagc aagctcccat cccacaagcc atacgagatt ccagagataa
     1981 ataggctctt gggtgatcat caaaggtttg attgatatac ttcatcattc ctggagcccc
     2041 acacgagtaa acagcatcaa actgactatc taaatcattg ataacaacgg aaacatttcc
     2101 cttgatgcca taagaaccat catctgtcgt tacaaagacc tgaccatact gagccaattc
     2161 cgttttcaaa ataacagcat ccttattagc aaaaccgagg actgtcacta ctttcactcc
     2221 acgttcatgc aattccttgg ccacctcaag caagggtgga acaccaatcc caccaccaac
     2281 aaggagaacc tgattctgct catcaaggtc agacaagtca aaaccatttc cctgaggccc
     2341 catcacatca agagtgtctc cctgacttaa ggttgaaaaa attgcagtcc cagctccctc
     2401 aatccgataa atgaggtgac actgcttgtt tgccttgtca atagacgaaa ttgaaatagg
     2461 acgacgtaag agatgggcat cgtccggtac acgcagatga agaaattggc ctgctcgcat
     2521 ggcttcaacc atttctcctt ctaggactaa ttcaaagatt gctggcgcga tttcctcttg
     2581 tgcaaccacc ttcatggttt ccaaccgaat gacacccaaa cgcttcttac atgtgggatt
     2641 cataactttt cctccttaaa ttttaagggg accagataaa gaaaagacct tgctagtgca
     2701 aggcctcagt taaaatcgta caacacaaga gagcacactc aaaaagtctc ctctagaaaa
     2761 ttgcactatt cttttatctg acaccttgtg agtctctctg gactcccctt aaaggttgaa
     2821 tttttattag tatactcttt cagctaaaaa aagtcaagta gaaaacgaat attctactta
     2881 acttgcacga aattattttt cacgaatgac ttcgacctta tatccatcag ggtctttgac
     2941 aaagtaatag tttggtgtag ttcctggtag accatttggc tcagtcactt catagccttt
     3001 tgcactgtgt tcttgatgaa gtgcctcaag atcaggtgta ctgagggcga tatgggcaaa
     3061 cccatcacca accacatacg gaccgtgatc gtagttataa gtcaactcca actcatagtc
     3121 atcaccctca agacctagat agacaatcgt gaaggcatgg tctggaaaat ctctgcgacg
     3181 caattcttta aaaccaaaag catcttgata aaatgcgatt gatttttcaa gattttctac
     3241 tcgtaagcaa gtgtgtagca tttttgaagc catatttttc tcctttattt ttaaaaagac
     3301 tggacaatcc tgttccagtc tcatcagttg ttatttacca agttttgctt tagctgcatc
     3361 tgcaagagct gtgaaagcta ctgcatcgtt aacagccaag tcagcaagca ttttacggtt
     3421 aacttcgatc tcagccaatt tcaaaccatg catcaattgt gagtatgaaa gtccgttcat
     3481 acgagctgcc gcattgatac gagtgatcca caatttgcgg aagtcacgtt ttttctgacg
     3541 acggtcacgg tatgcatagt agtaagagtt cattacttgt tcttttgcag tacggaacaa
     3601 gatgtgttta gctccatagt aaccttttgc taatttaaga atacgtttac gacgtttgcg
     3661 tgatacaacg ccacctttaa cacgtgccat gttttatttc ctccaaatat ttcctagaat
     3721 tgtttactta cagtcaagct attatttcaa gcgagtaagc attgctttga tacgtttgta
     3781 atctcctgaa tgcaccatag atgctttacg aagatgacga cgttgtttct tagtttttcc
     3841 gtggaaacgg tgagaagtgt aagcacggaa acgtttaagt ccaccagaac ctgtacgttt
     3901 gaaacgttta gctgatgcgc ggtgtgtttt ttgttttggc atgatttttt ctcctttatt
     3961 taactttctg acaattattt tttgtcagtc gctggcgcca attgcatgaa catttgacgt
     4021 ccatccattt tagcacgttg ttcgatgatg gcaatatctt gagttgcttc agcaaactcg
     4081 gctaaaactt ttgcaccaat ctctttatgg gtaatcatac gacccttaaa gcgaatagat
     4141 accttaactt tatttccttt ttcaaggaac ttacgtgcat tgcgaagttt tgtatcaaag
     4201 tcacccttgt caattgttgg acttagacga acttctttca cagtaacaac gctttgtttt
     4261 ttacgttgtt ctttttgctt cttctggtac tcaaatttga acttaccgta gtccataatt
     4321 tttgcaacag gcggtttggc ttggggttga atcaatacta ggtcaacatt agcgttatca
     4381 gccaaagctt gcgcttcact gagtggcttg atgcctagct gttctccttc aagaccaatc
     4441 aagcgaactt cacgtacacg aatctcatca ttgatgaata agtcttgctt tgctatggtt
     4501 ttcacctctt ttgttttatt agagaaaaac aatagcggac tcgtaaatat acaagcccgc
     4561 acgttatgat agcgtttctt cgaaactttt catccgtagg gccaggcaac ttaatgtcac
     4621 aaggcgagaa gctctcactt ctgcttttct caacttctat atgataccaa gttttctagc
     4681 tcttgtcaag ataaaaagtt attttttgaa aagtttgtgt ttgtgtttgt attttggttt
     4741 ggttttcgaa tcaaaaagag agaatcaagt tgattccctc tctatgttag tttttcctta
     4801 acttccttat aagaatgcta agacctatta aaaacttcct ttttacgtga acttccccag
     4861 ctttcgataa atagaatccc cactagcaaa aggataatca ggcaaggaag taagaccatc
     4921 cccatgctcc aatttagatc gacattatca atctgcttag ataatcttcc agataaaatc
     4981 tccactgaac gcaagtaagt aaagggaatc agatgtgcaa tcctttgaag aggctgaatt
     5041 gtttggatgc caaacaataa gccaacaatc ccaatgagtg aaagaaagag gacaggcatt
     5101 ttttgcttga aaaagtaagc aatcaagtac acaacttcca caatgacgat aaaggctaag
     5161 aaagctaaga gcaagccagg aaataataca tcttgtattt tcccaatagt tacttcttga
     5221 ttcactaagc tataaattgg gtagggataa tctaactgtc caaaaccact tatcagactt
     5281 cccactagaa aagaaaagcc acagattccg ataaacagca cagttacata tcccactcca
     5341 actccaagag aggatattgc aaatgtcact tttgaaacag gatataagtg agctgtgtcc
     5401 agatgatttt gatatctttc tgcaaatagt tgtgttagca taaaaataat cgcaaccaca
     5461 aacaagcttg ggatgataac ctctaaaatc cagacaatct gatcaatccc gtgggtcgga
     5521 aactccaaag tatgtgcttt tatgttcaag ggatacaggg cttggtaaat cttccgttcg
     5581 cggtcaaccc ccatttttaa gccagagcta gcagtcgggt catttgatac aaattcataa
     5641 ttcttctctt catcttgcca ctgcagatag taggcttctt tccagcgccc ttcttttagt
     5701 aaagttagaa tttctgtctt tcgcgtcaaa agattttttt gcacgtctaa attattttta
     5761 gcaaactggt attcctccga gctggtatca gacatttggg agagtttctc ttcattttca
     5821 ttgatagccc tctcgttggc tgcaatgcga ctttccaacc tgctctccaa gctgtgtgag
     5881 tttgcagtct gactatttaa ataaaaggta acaccgagta cagatgcaaa taaaagtaag
     5941 ataatccagt ttaaacgact tttgaaaact ttctttaaca aaaatagact aacatctttc
     6001 ataaactaaa cctcttctat ctgcccctga tgaatggtta ctactctatc gcagacatca
     6061 accaactctt ccttatagtg ggaacttaaa agaaccagct gttcttgtct atcgatttgt
     6121 gctagcctat caaaaaactt ctgtcgataa tactcatcta agccatttgt aatctcatcc
     6181 atgagccagc atttggcctg actgaggaaa tacatggcaa tcaccaagcg ttgcttcatg
     6241 cctaaggaat acttgcgaat gggaagactg atatagtcag acatttccca ataggcgatt
     6301 tcatccctca agttcagacc tgacttccag atgtttttga taagacgaag gtagtccatc
     6361 ccacttaagt ttccatccag ccattcaata ctctcataat aaaataaaga aggaggggct
     6421 gcgatatttc cactacttat gggaattaaa ttgctaatgg cacggaacag ggtcgtcttt
     6481 ccagagccat tgatagcaag aagaccataa accctaccct ttttaaaggt gaaatccaca
     6541 tcttgcaaga tgacttgtcg cgtttttaag gtcacatgag taagctgcaa catatctagc
     6601 cctccttttt ctcactcttt aagaattaat agcctccgct gtaatagttt atgacctcat
     6661 aacgaccgta gttccagcct ccgccaactt tatactcaga atagctgtca taacgagacc
     6721 atgacggaat ccaagcatac tgatggtcgt gatagttggt actatattcc caaaccgtat
     6781 tccaatcata cttgtaactt ttagtgactg tcacagcaga tacactggac tgaagaagac
     6841 caatagatta taaactaact aattaaacga tttttgctaa tttttaatga ttttatatcc
     6901 tcaatattga atacgttttc attataccat ttttatcatt attatgcaac aaatttttag
     6961 tttatttcac taatctacaa catttatcct tcctatcgaa cactttcgat tttccaacta
     7021 tccaccccct taaaacgtat agctccttgc tggtcagttc gataaacttt gctattgata
     7081 ccttccagtc gtgtcaatgt ttcctgatgg gggagtttca ttcgattgct ctttccaact
     7141 gagataagag taagctctgg tttgagtttt tctagaaagg ctggacttga tgatttttta
     7201 tttccttgtt ggctagcttt caaaacattt actttcaagt ctggatagtg cttcagcaag
     7261 tccttctctc ctttctcctc caaatttccc gtgaagagaa attgcttatc caagaatttc
     7321 ccatacagaa ctagggtatc atcgtgtcct ccatctccca ttttccttgg agatagaact
     7381 tctaactgac ttccaaaaat gggcaagttc tcccctactg tcatactacg cacctttgtt
     7441 tgagtcgcct gtagttctgc cacaaattcc ttctgtttca gactgtcttt tgatactaga
     7501 atctccccta catggaaagc cttggtcacc tctgacaaat ctccaacatt ctccttgtcc
     7561 gtgttagtca aaattagctg gtcaatctta gctactcctc gacttttgag atagggaatc
     7621 aaggttcgct gggcattgct ggtcgtcatc ttttcttgcc atttttcgat tttcttataa
     7681 gattctgcct taccacctac atctatgaga atggttttcc cagttacatc ccgtaggaaa
     7741 atactttctc cttgccccac atccagcatg gtgatttcat tttccagtgg atacttggta
     7801 aggaaaaaga gacctgtaat caataaactc aatactgtta atcctttaat gtttttcctc
     7861 aaatcataga ccaaagccaa ggaaattaac aataagatta aaagccatgc attgggttga
     7921 ccaaagacaa gcggtctcct tgccacctgc gagaccaagc gaataatgcc ctctaaccat
     7981 tcaaagataa agttcagctg aatgactgga tagagaaagg aaaggacaaa taagatagac
     8041 aagagcggta agaagaccaa gtcaaaaaga aaggaaaaga caaaggtcaa aaggatagac
     8101 caaggttgaa attccgcaaa atagaaggat agaatgggca atatgcccaa ggagatgact
     8161 agactttcac tagtaacagc cttgagcccc tccccttctt tgctggtcat ggtcaggata
     8221 aaagcataag cacaggacaa gactcctcct gctgtcaaga aaaagtttgg catgacaata
     8281 aagaggacaa gcaccgtcaa ggcaaaatta tccaagccct taaccccatg ttgagccagt
     8341 agcttttgca agagactgcg aataaccgat gctgaaaatc cagttagtcc cgcatagata
     8401 agggaaaagg gataagtcag ccacttcaac ttttcttgtg tcaagcccaa tcgtaaaagt
     8461 agtttcttaa atccgtccat gaaaaaccct acctgcatac ctgacaaggc aaaaaggtga
     8521 ataattccta aactggaata aagctcattc atctcctcaa aatcggtgtc cagatgtcct
     8581 agcaagagtc ctgtcatgta attgcgcata gggtctggga agtgcatctt aatccaaacc
     8641 acagcctttc gacgtaaact ggacaggttt tcacctatat cccaactgcc aaccttttgg
     8701 aatgactgga ttcttttaat attgagtgtc tggtaaattc cctgagtctt cagatagcct
     8761 tggtagtcaa agccaccaaa atttctctgc ccttctggct ccgaaagctt cccttctagt
     8821 cctatctcat gaaggtcagt taaagcttga aaggcttctt tctcctcctc ggactggagt
     8881 ttataataga cttggaaagc acgaccatca gacttaccac gaaaggacag actgtcaccg
     8941 ttaaccttaa tagtgtctgg taaaatccgt accctttcaa cagaatccac caaattttga
     9001 ctcacttgac tctgttgcca agtttgaaac agaaaccaga agccaaagac tccacaaatc
     9061 actagaactt tacctgctga tttccaagga aattggataa agaggcagac tagcagaaaa
     9121 acaaaaccca acaaagcaaa ataggatact gagaaaatgg cgtagtaaag ccatagtaac
     9181 agaaaactca ggtaaattag gggaatagag aaattcttaa tccactgtaa catagtcttt
     9241 aagtttttct atggtcttgg caccaatgcc agagaccttc ttgagctcgt ctactgactt
     9301 gaacttgcca tttgcctcac gatggtcaat aatgtcctga gctcgttttc ctcccagtcc
     9361 cttgacctgc ttgagttctt ccagactggc cttgttgaga ttgaccttct tttccttgct
     9421 tgttgaagaa gctgtccccg aaccagtctg ttgactaact gcttcttctc ccttagtagg
     9481 aacgtaaacc agagcctcat cactaacttt ctgagctaga ttgagcgact tgctgtctgc
     9541 ttgctctgtc aagccaccag ccttctgaac agcatcattg actcgactac ctacaggcaa
     9601 gtcataaatc cctggcgatt tgacagcacc tttgacatct actgtgatta gatcttgttc
     9661 aactggttct tccttctctt ccttgttcac ttcctttccg gtcgatgagt ccttggaaac
     9721 agctgcaact tcagcctgca aattcgtctc tttgacagat gtttgtggag ctggctttag
     9781 caggaaaaat ccgcctacaa gcaagcccag accagtacag atgacgatga ttttatactc
     9841 tttgattttc tcgataattg cttccatatt ttctcctctc ttagattatt cgtaagagga
     9901 agaaaaaaca gtcgaaaaat ttcttttcaa ctgcttattt atttgcaaaa tagtcttcct
     9961 tggtcaagac ataatgaact cttgtcacga ttcggccttt ttcatgctgg tccatacaag
    10021 catatggttc tgcatgggaa aaacgcatgc ctgatttctc catgaccttt cctgacgcgg
    10081 gattagcctt atcgtgaagg gcagtcaact tattcatccc tatcttctca aaagctagct
    10141 caatcacagc acgattggct tctgtcgtta atccttgatt ccaatacttt ttattgataa
    10201 tgtagccaat agctgccttc ttaagaacag gatcaatctt gtgtaagtca atagttccga
    10261 taaacttgcc gttatctttt aattcaatcc cccaacgtcc caagggatta gccaagtaga
    10321 actgagcaat gttattcttg gtttcttcca agctttgatt ggttggaaaa gtgtaacgtg
    10381 tattaccctt gtccgaggca tagtcaaaca ttgcttccgc atcatccaaa gttacaggtc
    10441 taagcaataa acgctccgtt tctatagacg gatactgggc aaatttcaca aatattgatt
    10501 ccatactttt ccctccatta gagcttgatt ttcactagtc tagcataaaa aaatttggat
    10561 gacaagtttt tcttgatttt tgaagcggtt tcatatacaa tgaaaccgtc acatttttga
    10621 tttatgaact ggaatgtgac aaccaaaaca agaccgtcaa ttgaaaggta ggttatctca
    10681 atgttaatcg gaatcccaaa agaaattaaa aataacgaaa accgtgtcgc cctcacacct
    10741 gcaggtgttc atagcttagt tagtcgtggt catcgtgtcc ttatcgaaac aaatgctggt
    10801 ctcggttctg gctttactga tgctgactat caaaagcaag gagctgagat tgtcgctact
    10861 gctggtgaag cttgggcagc agagttggtt gtgaaagtaa aagaaccttt aagttctgaa
    10921 tacggttact tgcgcgacga tcttcttctc ttcacctact tgcacatggc cgctgctcca
    10981 gaattagcag atgctatgtt agcagcaaaa acaacaggaa ttgcctatga aactgttcgt
    11041 gacaatcaag gacaactacc gctcctcgtt cctatgagtg aggttgcagg tcgtatggct
    11101 gttcaaatcg gagctcactt ccttactaag caagctggtg gctctggtgt tctacttggt
    11161 ggtgtaccag gtgttccaaa aggaaaagta actatcatcg gtgatggtgt cgtcggtaca
    11221 catgctgccc gcatcgccct tggtcttggt gctcaagtga ctattttaga tattagtgcc
    11281 aagcgtctct cagttctaga agaagtcttt ggaagtcaaa ttcaaactat tatatctaat
    11341 tcattcaaca ttgaagcaag tgtgagagat gctgatgtgg tgattggagc cattctcatc
    11401 cctggtgcaa aagcaccgga attggtgaca gatgagatgg tcaaacaaat gcgtccaggc
    11461 tctgtatcgt tgacgttgct gttgaccaag gtggcgttat cgaaacagct gaccgtgtga
    11521 caacgcacga tgaacccgtc tatgaaaaac acggtgttct ccactatgcc gttgccaata
    11581 tccctggtgc ggttgcccgc acttcaacca tcgccctaac caatgtcact cttccttata
    11641 tcgaagcttt ggctggcaaa ggattcgcac aagcaatctc tgaagatgaa ggcttgcgtc
    11701 aaggtgtgac cacttatcaa ggttacttga ctagcctacc agttgctcaa ggacttaatc
    11761 gtgactacac tgatatcaat gatttagtat aaattttgaa aagaaaaggg tgacttcgca
    11821 agtcctaccc actgtgcttt gtgtcatcag ctattacatc gttgatttat tgtactgtat
    11881 actttgcaac tggagacttt cgttccagac tcaattgtgc ataagaaact ctaaattgaa
    11941 taaagtcaac agagtttcgc ccaaccactt cgtcttgtaa tgacaattat ataaaccaaa
    12001 ggaagctgag ctctttagtc ccagcttctt tttatatata aaattttacc cgtgcaaaga
    12061 cagggcctta gcagacttct tttttacttc gttcaccctt gctttttctt tttaggtttg
    12121 ggcgttggca gttggttata catagctaaa atcaggtctt atagaaacat cttattatca
    12181 agttcttcca ctcaaatcat ttctttggca cctttgtatg gaaactcaaa agaagattgg
    12241 tcaatcttat ctaagactgc ttgcacgggt ttaactaaaa agcgatcgtc ataaatgccg
    12301 ccaataatct tgccgcggaa gtaaagaata tactccccaa tcatggaacg gtaagtcaca
    12361 tcatctaatc ctgataattg ttccaaaaca aattccaaat agttcttact tgatgccatt
    12421 tctaatcttc taggctctgt tcagcgataa caaccgtata gagttcttgc ttaacctcgc
    12481 atccaattga tttaaagccc tgcttttccc aaaaatgctg agattgcgga tttcccttaa
    12541 cataagccaa acgtgccttt cgaaagttct tagcaaaata agctagtgct tctgtcacaa
    12601 tatgactacc aatccctttc ctctgatagg cttgatcaac cataaacaaa ccaataaaaa
    12661 cagtctcctc atcaggatat gcatagacaa aatccataac agccacaagg tcagatccat
    12721 tccaaaatcc aacaaaaaac ttatcagcct tagctttacc ttcaggtaga caaagcatgt
    12781 cctcttttac agttgcaaaa tttggctctg gtggacaatg ctgaaaatac agaggattac
    12841 tttcatataa agataaaata cttggaatat ccttttcagt tagtatccta caactgtaat
    12901 acttagatag ttggtcaatc atcttttcaa attcctactt tcttgcgccc tgtgattatg
    12961 acacaggaag atgcactgat cgtcatcagc cacataaaag ttctttccat cgtgcctaat
    13021 cgttgtctca aacctttagc ctatacaact ggattttcct ctctcaaaag tatattcttt
    13081 tgcaggcgaa cttcctcaaa atcagtcgtg tgcaacttca gtagaatatt cataggctcg
    13141 gataatctga gcgacaactg gatggcgaac cacatccttg gctgaaaaat gaacaaagtc
    13201 aatctgatgg atgttcttga gtttatcttg agcatcaatc aaaccggact tgacattacg
    13261 tggcaggtca atctgactga tatctccatt gacaatcatc ttagaatgaa agcctaaacg
    13321 cgtcaagaac atcttcatct gcatgatggt cgtgttttgc gcctcatcga gaatgacaaa
    13381 ggcatcatcc aaggtccggc cacgcatata ggcaaggggc gcaatttcga taatttcacg
    13441 ctccatgaga cgagtcgttt ggtctttccc aagaatttga tacaaggcat cgtaaacagg
    13501 acgaaggtaa ggatccacct tctccttaag atcacccgga agaaatccaa gactctctcc
    13561 cgcttccacc gctggacgag ttaggataat tcgcttgact tgcccacgtt taagggcagt
    13621 cactgccaag gtcactgcaa ggaaggtctt ccctgtacct gctggcccaa ttccaaaggt
    13681 cacatcatgc tgtttgacac tgtccacata aagtttttga cctaaggttt tgacgcggat
    13741 aggcttccca gtattatcct tgatgatttc ttcttcgtaa agggcgacaa acttgtcaat
    13801 ttcatcattt ttgaccatac taatagccgt cactacatct ggcgtaccaa cggtcatccc
    13861 acgatttacc aaggccatga gagcttgaat aacctgacgg gcttcctcgc aagcagactc
    13921 ttctcccaaa acctggacaa tctccgtacg agcatgaatc acaacatcaa gctcttcttc
    13981 catcaaacga agatggcgtt cattggaacc aaaaagatga aacaggtcat ctggatgact
    14041 cagttgaatg tctattgaat gttccttcaa ataaagaacc tctctaatcc ttttatttct
    14101 ttttattata gcagagggat ggagaaatta ctagcccaaa cccaatgaat aaatgctagt
    14161 gttctagata ttcctgccaa atgctgtcaa aatctcctag gttaaaagag aaactggcat
    14221 gctcctccaa aaagcgactg acctcatcaa aatcatctgt atgttttgga aaagctgcct
    14281 cttcaaaagc gaggtctgcc aaaattgctt tgggattgtt acttttagga ttgcgctcgg
    14341 tcatgagcca agtgtaaaat gattttctca taagcctccc taaatcaact ctgtaccaaa
    14401 ctggtcctgc ttgattttac cagccttcat aagaccacct aaagctttct tgaactgacc
    14461 tttagaaatg ccaaaggttg ccttgatgtc gtctggagat gacttgtcat ttaaggtcat
    14521 gaaaccgcca ttgctttcca aataagtcaa aatcatctga gcatcgtttt ccaacatttc
    14581 aaaggagcgt ggtttgaggg agaggttcaa agtgcggtcc acttcacgga aaccaataac
    14641 gcgcgcatct aatacttgcc ccaaacgtgg ctctgcgtaa cgctcgctag gatgaataaa
    14701 accaagcata ttattttctg gtaggtaaac aaaagttcct gatagcttga gacggtaaac
    14761 aatggctggc cagttttggt tctgcatgtt gttgtaggca ggacgagcaa gacgttggaa
    14821 gtcttcttga taagccaaga gaccccagat acggtctttc ttatccactt caagacggat
    14881 gtagagttgg tcgcccttct taggccagag ttccttgagc tcagggagaa tatcgagtga
    14941 cacaacgatt tccttgtcag gaaggcctgt atccacaaag acacccaagt ccttacgaac
    15001 ctctgtgaca cgtccccaac caaattggtc ctgagtggca gtcacttcta agcttgtcag
    15061 gcggagtttt tgcttcatat ccgtgtatgc aaaacctttg accgtatccc ctactgtatg
    15121 ttggccttct tccttagcaa gagcataggt ttgaccatcc ttttgcacaa agtaaaaacg
    15181 gtcgttttca tcgatgatca gtccaacgat aaaacttgca agatttgtat tcatatttcc
    15241 ttctttcgaa taaaactcag ccagcaatgc caactgagtt tttctgttta tttttagact
    15301 tccaaaagtt ctttctcttt gttggcagtc atatcgtcga tgtgtttaac agcatcgtct
    15361 gttacttttt gaatgtcttt ttcaagagtc ttcaattcgt cttcagtgat ttcttttgct
    15421 ttttcttgtt tcttagcttc gtccatagca tcgcgacgga tattgcggac agccacttta
    15481 gcattttcgc cgaccttctt cacttcttta gcaaggtcac gacgagtctc ttctgtaaga
    15541 gctgggacaa ccaagcgaat cacagaaccg tcattagctg gtgtgatacc aagatcagaa
    15601 gcgttcaagg cacgttcgat gtctttcaat gaagacttgt caaatggtgt taccaacaaa
    15661 acacgcgctt ctggaatcgt aattgaagcg atttggttaa gaggagtttc aactccatag
    15721 tattctacat gtatacggtc aagcaagctt gcattggcac gaccagcacg gataccacca
    15781 aattcacgag caagtgattg gtgagactgg gtcattctct ctttagcttt ttcaataatt
    15841 gcgttagcca tattctttct tattcctttt cttcgatatt atttgaaact gttgttccga
    15901 tattttcacc aaatacgaca cgtttgatgt tgcctgattg gttcatgttg aagacaacca
    15961 agtcaatgtc gttgtccatt gagagggttg aagctgttga gtccatgata cgaagacctt
    16021 tattgataac gtcacggtgg gtcaattctt caaacttaac agctgtctta tctttcttag
    16081 gatcggcatt gtaaacacca tcgacaccat ttttagccat gaggatggca tccgcttcga
    16141 tttcagctgc acgaagggcc gctgttgtat ctgtcgagaa gtatggtgaa ccaattccag
    16201 caccaaagat aacgatacgg cctttttcaa ggtgacgaag ggcacgtcca cggacataag
    16261 gctctgccac ttgttgcata gcaatagctg tttgtacacg cgtatcaacc ccaacttgtt
    16321 gcaatgaatc tgccatcaca agagcattca taacagtccc aagcattcca gtgtaatctg
    16381 cctgaacacg gtccatacct gcttctgctg caggttctcc acgccagaga tttcctccac
    16441 caataacaag ggcaatttcg atacctaagc tatgaacttc ttgaatctct tttgcgattg
    16501 tttgaactgt ttggatatca atccctacgc cacgttcacc ggcaagggct tcacctgata
    16561 acttgattaa aatacgttta tacttgggat tcgccatttt cactctcctt ctttcatcct
    16621 acctatttta tcacaatttc taagattttt atagtatcat gaacaattct ttcaaaaaaa
    16681 ttagacagtc aaaaattcct ctaagtcggc aagggcacgc tctgcaattt tttcataacg
    16741 agccttctta tcacggatac gctcgccttc caactccttg atgatcccaa aattgacatt
    16801 cattggttgg aaatgtttgc tgtcggcatg ggtaatgtaa tgagctaagc ttccaatcgc
    16861 tgtcgtctcg gggaaaataa cctcgctttc ttccttgaag agacgagctg cgttaattcc
    16921 cgcaactaag cccgaagccg ccgactcaac atagccttcc acccccgtca tctgaccagc
    16981 aaagaagaga tttggttgtt tcttggaacg gtaagtctgc tcaagaagat ttggtgaatc
    17041 catgtaagaa ttgcgatgca tgacaccata acggacaaac tccgcatttt caagacccgg
    17101 aatcatttgg aagacacgct tttgttctcc ccatttgagg tgggtctgga aaccaacaat
    17161 attgtatagg ctaccagctg cattatcctg acgaagttgc acaaccgcat aaggtgtttt
    17221 aaattctcca tcacgaggtc ctgtgtagtc gtccggatac tcaagaccga ctggcttcat
    17281 agggccataa agcatagttt taatgccacg tttggccata acttcgattg gcatacatcc
    17341 ttcaaagtac ttttcttttt caaaagaatt gagcggtgct tcttccgcat tgaccaaggc
    17401 ttcatggaaa tccataaact cttgcttggt cattggagca ttgagatagg ctgcttctcc
    17461 cttatcataa cgagatttga gatagacctt actcatatcg atagtgttga catcgataat
    17521 aggcgctgct gcatcgtaga aatagaagcc atcgccatta ttaagggcat gaatcttttc
    17581 agccagggca tcgctagtca agggaccagt cgccacaact gtaataacat ctgttggcaa
    17641 ttctgtaatt tcatcacgca ccacttcaat caaggggtgg ttgaccactt tttcggtcac
    17701 catttgagag aaaccatcac gatccaccgc aagggctcca ccggcaggaa cacgtgtagc
    17761 ctcagcagat tccaagataa cagaacccaa gcgacgcatt tcttccttga gaagaccaac
    17821 tgcatttgtc aaagcatccc cacgcaaaga attggaacaa accaactcag caaaattgtc
    17881 tgttttatgc tggggtgtag acttgacacc acgcatttca tatagtttaa ctggaatacc
    17941 acgctctgcg atttggtaag ctgcttcaga acctgccaaa ccagcaccga taacattgat
    18001 ataagattga gacacgacac taatacctct ttgggagtgt gaagttaaga ttcacattga
    18061 aaaagccaat cagacttaca agctttcgag tttcttggct caggctgaaa aagtccattg
    18121 gacttacttc gctctcgaat ttcttggctc aggctgaaaa agtcccccgg actttcctcg
    18181 ctctcgaatt tcttggctca agctgaaaaa gtcccccgga ctttttcact cccacaaatc
    18241 tttctatttt tttcttctac tagtataaca aaaaaaggga agaaggcaaa cttccctgtt
    18301 tagtcatttt cttgatgtaa agagatagag agtattccag ttaagaatca attatatgct
    18361 actctataaa atcttttcca cataacggat cgatagggac tgttattcta tctcttgcta
    18421 taaccgtatt atctaaaaca gcataacatt caacatagtg ctctccttta aactgagaat
    18481 cttcgtgatg ttttatttta cctgaaaaaa tagcaccacg ctcacaattt cttctaatcg
    18541 cctcagctcc aatatttctt actttccaat agacttcctg cgaaacaaaa tatggtatag
    18601 tagttctatg aattatgaag caagtaaaca actaactgat gcacgattta agcgtcttgt
    18661 tggtgttca
//
LOCUS       DAVBDG010000039        17926 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBDG010000039 DAVBDG010000000
VERSION     DAVBDG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.040"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            77..502
                     /locus_tag="VQ526_001372"
     CDS             77..502
                     /locus_tag="VQ526_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2555087.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            513..881
                     /locus_tag="VQ526_001373"
     CDS             513..881
                     /locus_tag="VQ526_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2555088.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            988..1632
                     /gene="pcp"
                     /locus_tag="VQ526_001374"
     CDS             988..1632
                     /gene="pcp"
                     /locus_tag="VQ526_001374"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2555089.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(1660..2517)
                     /locus_tag="VQ526_001375"
     CDS             complement(1660..2517)
                     /locus_tag="VQ526_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2555090.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            2647..3789
                     /gene="tgt"
                     /locus_tag="VQ526_001376"
     CDS             2647..3789
                     /gene="tgt"
                     /locus_tag="VQ526_001376"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2555091.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            3890..5707
                     /locus_tag="VQ526_001377"
     CDS             3890..5707
                     /locus_tag="VQ526_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2555092.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            5860..7011
                     /gene="nagA"
                     /locus_tag="VQ526_001378"
     CDS             5860..7011
                     /gene="nagA"
                     /locus_tag="VQ526_001378"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2555093.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            7174..8232
                     /locus_tag="VQ526_001379"
     CDS             7174..8232
                     /locus_tag="VQ526_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2555094.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            8383..8748
                     /locus_tag="VQ526_001380"
     CDS             8383..8748
                     /locus_tag="VQ526_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2555095.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            8824..9765
                     /locus_tag="VQ526_001381"
     CDS             8824..9765
                     /locus_tag="VQ526_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2555096.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            9713..10729
                     /locus_tag="VQ526_001382"
     CDS             9713..10729
                     /locus_tag="VQ526_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2555097.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMAGLVSVLALLALTFYKRSSKMNVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            10731..11057
                     /gene="comGC"
                     /locus_tag="VQ526_001383"
     CDS             10731..11057
                     /gene="comGC"
                     /locus_tag="VQ526_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HET2555098.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQVKAYKEYNDKNVGA
                     NRKVND"
     gene            11050..11454
                     /locus_tag="VQ526_001384"
     CDS             11050..11454
                     /locus_tag="VQ526_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2555099.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTISNGSQKLPVPKGIQVPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            11459..11719
                     /locus_tag="VQ526_001385"
     CDS             11459..11719
                     /locus_tag="VQ526_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555100.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            11682..12143
                     /locus_tag="VQ526_001386"
     CDS             11682..12143
                     /locus_tag="VQ526_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2555101.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            12121..12534
                     /locus_tag="VQ526_001387"
     CDS             12121..12534
                     /locus_tag="VQ526_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2555102.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     VKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            12671..13272
                     /locus_tag="VQ526_001388"
                     /pseudo
     CDS             12671..13272
                     /locus_tag="VQ526_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            13333..14286
                     /locus_tag="VQ526_001389"
     CDS             13333..14286
                     /locus_tag="VQ526_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2555103.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKHLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSNLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            14337..15527
                     /locus_tag="VQ526_001390"
     CDS             14337..15527
                     /locus_tag="VQ526_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2555104.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            15676..16047
                     /gene="rnpA"
                     /locus_tag="VQ526_001391"
     CDS             15676..16047
                     /gene="rnpA"
                     /locus_tag="VQ526_001391"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2555105.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            16022..16846
                     /locus_tag="VQ526_001392"
     CDS             16022..16846
                     /locus_tag="VQ526_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2555106.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            16865..17851
                     /locus_tag="VQ526_001393"
     CDS             16865..17851
                     /locus_tag="VQ526_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2555107.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
BASE COUNT         5458 a         3139 c         4134 g         5195 t
ORIGIN      
        1 tcttatttca tttcactata ctttatatta taattgattg gtcaactatt gataagtcaa
       61 tggataggag gaagaaatga tagagattca agatttactg tatcaactcc gcttgtctga
      121 gcaagcgagt acgcaattgt ttgaaaaaag gcttgggatt agtttgacac ggtatcagat
      181 tttactgttt ttgctggagc attctccttg taaccaaatg gcggttcagg agcgtttgaa
      241 aattgatcag gctgctttga cacggcattt caagattttg gaaacggaag gtttggtgga
      301 gcgtcatcgt aatcctgaaa atcagcggga agtgttggta gaggctgcga agtatgccaa
      361 ggagcagtta gtggtgaatc cccctctgca acatatcaag gttaaggaag agatggaaag
      421 tatcttaaca gagtttgaga gaacagaact cagccgttta ttaaataaat tggttttggg
      481 tattgaaaat atagaaattt aaggagaaat agatgtcaat tattacaatt attttaacaa
      541 cgatcgttgc tttggagcat ttttacattt tttatttgga aagtattgct acgcgatcag
      601 atgcgactag tcgtgtattt aatatggaaa aggaagaatt ggctcatccg tcagtaagtt
      661 cattgttcaa aaatcaaggg atttataagg ctctgctagg agtctttctc tggtatggca
      721 tttatttctc acagaattta gaaattgtga ctatttttgt cttatttgtg attggtgctg
      781 cgacttacgg ctctttaaca gcggataaaa aaattatttt gaagcaaggt ggatcagcta
      841 ttttggcctt gattagtatt ttactcttta aatacacttg aaggtcgatt ttaatctcgc
      901 taatcctttt taatccagaa taagggaaat atgttatact tgtttttaag aaaaaagtct
      961 cattgaattg gttttgagga gttagaaatg aaagtattag tgacaggttt tgagcccttt
     1021 ggaggggaaa agggcaatcc aactttggag gccattaaag gtttaccagc tgaaatccat
     1081 ggtgctgagg tccgttggct agaggtgccg acagtttttc acaaatctgc tcaagtattg
     1141 gaagaagaga tgaatcgtta tcaacctgac tttgtccttt gtattgggca agctggtgga
     1201 agaactagtg tgacacctga acgagtggcc attaatcaag acgatgcacg catttctgat
     1261 aacgaagata atcaaccgat tgaccgtccc attcgcccag atggtgcttc ggcctacttt
     1321 agtagtttgc cgattaaagc gatggttcaa gctataaaaa aagagggctt accggcctct
     1381 gtttccaata cggcagggac ttttgtctgc agccatttga tgtatcaggc tctctatttg
     1441 gtagaaaaga aatttccata tgttaaggca ggttttatgc atattcctta tatgatggaa
     1501 caggtggtga acagaccgac tactccaact atgagtttag tggatattcg gcgagggata
     1561 gaagcagcaa tcggcgctat gatagaacat ggagatcagg aactcaagtt ggtaggcgga
     1621 gaaactcatt gatagaaaaa agcttgaggg gaaaaacctt caagcttttg gacgttttcg
     1681 agccaatact gctcggtaaa acataatttt agtgcattgg atataaggta ggagtgaaaa
     1741 actagcaatg ccaaaggtaa tccaattgag gaagtaccaa ggaagaagct gtaaatctag
     1801 gacaaagtgc tggaacttgt agcccttcat aaaggaacgg ctagttttta ggattcgtct
     1861 tggtgggacc tgtcctaggt ctagactata acagagaaga aattccacct gtgaataggc
     1921 ataatactgt ggaatataga ggatatttcc tacaatgatc aagatgagac ttgcaagaaa
     1981 gtagagtcca aagaccatga ggaaacgctc ggtttcaact gatgagagat ctagatttgg
     2041 aaactcagga tgtagggtga cgaatttttt ggctaaaaag ctactataaa agaggaggta
     2101 aatcccaagt aaattaggga tactccataa aaagagatag aaacgtttga gaagtagggt
     2161 caaaaaggtt tgagaaaagc gctcctcatc aaagagagct aggctgtttt ttacagatgg
     2221 ctccgtttta gaatctttca tgagtgtcag tgttgcatag acggaactgg tcaaaagaat
     2281 agtcccgata aaggagacta gtagaggaaa gaggtaggtt tgaagtattt ggccaagtat
     2341 gctgaaaaat ggctgttcta aaacagtccc gtggatccga gataagggat taagaaaacc
     2401 agataagatg accagcatac tgggaaggat atagaggaga aagagacggg gggtgtcagc
     2461 ctgaaaatgt tttgactcct gacgaattgt ttttaaatca atttttggat agttcattct
     2521 cttattatac catagttctt atacatagtt cgtgacagtt cctacttttt ttgataaaat
     2581 catacagtgt gtccttgggc acactgtatg aactgggact gtctttccca gcttcggagg
     2641 taaaaaatgt cagattcacc aatcaaatat cgtttgatta agaaagaaaa acacacagga
     2701 gctcgtctag gagaaatcat cactccccac ggtacctttc cgacacctat gtttatgcca
     2761 gttgggacac aagccactgt caaaactcag tcacctgaag aattgaagga gatgggttcg
     2821 ggaattatcc tatcaaacac ctatcatctc tggcttcgcc ctggagatga actcattgca
     2881 cgcgctggtg gtctccacaa gttcatgaat tgggaccagc ctatcttgac agatagtggt
     2941 ggttttcagg tttattcttt agcagatagc cgtaatatca cagaagaagg agtaaccttt
     3001 aaaaatcatc taaatggttc taagatgctc ctatccccag aaaaagccat ctctattcag
     3061 aataatctgg gttcagacat catgatgtcc tttgatgaat gtcctcagtt ttatcaacct
     3121 tatgactacg ttaagaaatc gatcgagcgt accagccgtt gggctgagcg tggtttgaag
     3181 gctcaccgtc gtccacatga ccaaggtttg tttggaattg tgcaaggtgc aggatttgaa
     3241 gaccttcgcc gccaatcagc tcacgatctt gtcagcatgg atttctcagg ctactctatc
     3301 ggtggtttgg cagtgggaga aacccatgaa gagatgaatg cggtcttgga ctttacaact
     3361 caactgctgc ctgaaaataa acctcgttat ctgatgggtg tgggagcgcc agatagcttg
     3421 atcgatgggg tcattcgtgg ggtggatatg tttgactgtg tcttaccgac tcgaattgct
     3481 cgtaacggga cttgtatgac cagtcaagga cgtttggttg tgaaaaatgc ccagtttgct
     3541 gaggacttta cgccactgga tcctgagtgt gattgctaca catgtaataa ctatacacgc
     3601 gcttaccttc gtcacctgct caaggctgat gaaacctttg gtatccgctt gactagctac
     3661 cacaatcttt acttcttgct taacctgatg aagcaagtgc gacaagccat catggatgac
     3721 aatctcttgg aattccgtaa gtattttgtg gaaaaatatg gctataataa gtcaggacgt
     3781 aatttctaaa atggaattga tataagctaa aaatcctaag ttttctctta ggatttttct
     3841 tctttttttg atagaataaa gtgtacaatg aaaggaagaa taaactcgta tgcgcattaa
     3901 atggttttcc ttgattagga ttataggttt acttttggta ctcttgtatc acttctttca
     3961 gacgatcttt cctggaggat ttttcggggt agatgtcttt ttcacatttt caggtttcct
     4021 gattacagct ctactcattg aagaattttc taagaacaat gagattgatt tgataggatt
     4081 ttttagaaga cgcttttatc ggattgtgcc acctgtggtt ttgatggtct tggtgaccat
     4141 gccttttact ttcttggttc gccaagacta tgttgctgga attggtggcc agattgcggg
     4201 cgtcttaggc tttatgacca acttctatga actcttaaca ggtgggagtt atgaatctca
     4261 gttcattcct catttgtttg ttcataattg gagtttggca gttgaggttc actactatat
     4321 tctttgggga ttggcagttt ggttcttatc caaacaagct aaatcaaatg gtcagttgaa
     4381 ggggatgatc tttctcttat ctgctactgc cttcttgatc agtttcttct ccatgtttat
     4441 tggtagtttt ctagtgacct cttattcctc tgtttatttc tccagtttaa ctcatgtcta
     4501 tccattcttt ttgggaagta tgttagcaac tattgtaggc gttcgtcaga cgacttccct
     4561 cgtcaagcag ttggataaaa tctgggattt acgaaagact ttagtagttt ttggaggagg
     4621 ctttggtttc ctagttcttt tgactttctt tgtcaaattc acttatcttt ttgcctatct
     4681 tatcggcttc ttgcttgcca gtcttgcagc tcttgccatg attctggcgg cgcgtgtctt
     4741 acatgaaaag acacatcata tacaggagcc gaagattatc agctttttag cggatactag
     4801 ctatgcggtt tatcttttcc attggccttt ctatatcatt ttctcacagt tgacatcaaa
     4861 tcttcttgct gtattactga ctctgatttg ttcttatggc tttgccagtc tgtcatttta
     4921 tgtattggaa ccttggattg caggcaagaa cacacctatt ttacaaactc ttcgtcccct
     4981 gccttatatt cacgcaattc ttgcagcagg tacaggaatc ttgaccttca ttgtctgcat
     5041 ggtgaccttg ttggcaccac aagtgggagc gtttgagaca gacttgactg tcaatggctt
     5101 gaagcaagct gcaacaaata ttggccagac caaggtgatg gcagaacggg cagatgcaaa
     5161 cagtttggga attgctgatg gcactatgtt aattggtgac tcagtggctt taagggcaaa
     5221 tacagcacta cagacagctc ttcctggagc acagattaac gcgcaggtca gcgtaacaac
     5281 caagaccgca aatgaaatca tgctaaataa tagccagaat aaatttttac ctaagacggt
     5341 ggtcattgcg actggggtaa ataatcctga gaattacaag gatgactggg acagtatcgt
     5401 gaaaaatctt cctaagggac accatatgat tttggtgact ccttatgagg gtgataagac
     5461 aaaagagacc tatgccatcg ttgagaaggc tgctgcctat atgagagaat tggcagagaa
     5521 gacaccttac attacgatag cagattggaa tcaagttgcg aaagagcatc cagaaatttg
     5581 ggctggaaca gaccaggttc atttcgggag tgagagtagc actatcgaag caggagcaaa
     5641 attgtatgca gatacgattg ccacagcttt gcagacagct caagacaagc cggttaaatc
     5701 aaaataactt gtattaaaaa agagaagagt tggagaaatc cagctctttt aaaatatctc
     5761 caaaaaatag gtctatacca tttacaaata aaaaagaaag gtttataatg taattgacat
     5821 aataaattgt agaatcaatc ttttaaggag gttaacatta tgcctaacta tattaaagcg
     5881 gatcagtttt tctacccaca cggagttcgt cgaggtggtt acttggaact tgtggacggc
     5941 aagtttggaa aacatgtaga gcagattcct gaaggggctg aggtgattga ctatacaggt
     6001 tatagcattg ccccaggtct tgtggatact catattcatg gatatgcagg tgtagatgtg
     6061 atggacaaca acattgaagg tacattgcat actatgagtg aaggacttct tagtaccggt
     6121 gttaccagtt tcttacccac aactttaaca gccacttatg agcaattgct tgcagtcact
     6181 gaaaatcttg gaaaccatta taaagaagca acaggtgcta agattcgtgg gatttattat
     6241 gaaggtccat atttcacaga aacttttaag ggggcacaaa atccaactta tatgagagac
     6301 ccgggtgttg aggagtttca ttcttggcaa aaagcggcaa atggcttgct taataaaatt
     6361 gcccttgcac cagaacgtga tggggtggaa gactttgttc gtacagttac gggcgaaggt
     6421 gtgacggttg ctcttggaca ttcaaacgcg acttttgatg aagccaaaaa agcagtcgat
     6481 gctggagcga gtgtttgggt gcatgcctac aatggaatgc gtgggttgac tcaccgtgaa
     6541 ttgggtatgg ttggagccat gtaccaattg ccacatacct atgcagagtt gatctgtgat
     6601 ggtcaccacg tagatccaaa ggcttgcgaa attcttatca aacaaaaagg aacagaaaac
     6661 atcgctctta tcacagactg tatgacagct gggggattgg aagacggaga ttatatgttg
     6721 ggagaattcc cagttgtcgt tgcaaatgga actgcacgcc tcaaatcgac aggtaacttg
     6781 gcaggttcta tcctcaaact caaagatggt ttgaagaatg tggtcgaatg gggaattgcg
     6841 aatccgcatg aagcagtcat gatggccagc ttcaacccag ctaaatccgt tcacatcgat
     6901 gacgtctgtg gccaaatccg tgaaggctac gacgctgact tcatcgtatt agataaagat
     6961 ttggaattgg tagcaaccta cctagatggc gtgaaacgtt atcaagcata agagagaagg
     7021 cagaagttag agactaactt ctgctttttt atacagggta ctttactggt aaataaaaag
     7081 ttaaaaaaat cacaaaaaaa gcttgaagaa acaaagaaaa atgcttacaa ttgaactata
     7141 aatattacaa ataaaaaaac ggaggagtgc tttatgaaag cctatactta tgttaaacca
     7201 ggacttgctt cttttgttga tgtagacaaa ccagttattc gcaagccaac agacgctatt
     7261 gtgcgtattg taaaaaccac tatttgtgga acagacctcc atattatcaa aggggatgtt
     7321 cctacttgcc aaagtggtac cattcttggc cacgaaggga ttgggattgt tgaagaagtt
     7381 ggggaaggag tttccaactt caaaaaaggt gacaaggtct tgatttcttg cgtctgtgcc
     7441 tgtggtaaat gctactactg taaaaaagga atttatgccc actgtgaaga cgaagggggc
     7501 tggattttcg gtcacttgat tgatggtatg caggctgaat atctacgtgt ccctcatgca
     7561 gataatactc tttaccatac tccagaagac ttgtcagatg aagctttggt tatgctgtca
     7621 gacattctgc ctactggata tgaaattggt gtcttaaaag ggaaagtaga acctggttgc
     7681 agcgtagcca ttattggttc aggtccagtt ggattggctg ctcttttaac agcccaattc
     7741 tattcaccag ctaaattgat tatggtagac ctagacgata accgcttgga aactgcccta
     7801 tcattcggtg cgactcataa ggttaattct tcagacccta aaaaagccat taaagaaatt
     7861 tatgatttga cagatggtcg tggtgtggat gtcgctatcg aagctgtagg gattcctgaa
     7921 acatttgatt tctgtcaaaa gattatcggt gtagacggaa cggttgccaa ctgtggtgtg
     7981 catggtaaac cagttgaatt cgatttagat aaactttgga ttcgcaacat caatgtaaca
     8041 actggtttgg tatctacaaa tacgactcca caattgttga aagcacttga aagtcataag
     8101 attgaaccgg aaaaattggt aactcactat ttcaaactca gtgaaattga aaaagcctac
     8161 gaagtcttca gtaaggcagc agaccaccat gccattaagg tcattatcga aaacgatatc
     8221 tcagaagcct aagtagtaaa aatatttttg tacataagca aatagaaatt cagtcatcca
     8281 tcagatggct ggatttttta tcaaaaaatt aagaaatgag catatttctt tccttgtctg
     8341 gcggaattgg ttataatata cggtacaaag gaatgaatga atatgtatcg tgttatagaa
     8401 atgtacggag attttgaacc gtggtggttc ttagaaggtt gggaagaaga tattgtagca
     8461 agtagaaaat ttgaccagta ttatgatgct ctcaaatact acaaaacttg ctggtttaga
     8521 ttggaacaag aatcccctct ttataaaagt agaagcgact tgatgaccat tttttgggac
     8581 ccggaagacc aacgctggtg tgatgaatgt gatgagtatt tacaacaata ccattctttg
     8641 gctcttttgc aggatgagca ggttatccca gacgaaaaac tacgctcagg ctatgaaaaa
     8701 caaaccagtc aggaaaggaa tcgttcttgc cgtatgaaat taaaatagag aaaagtaact
     8761 tttttggagt tgcttttttt atttttctaa cgctttgcga atagtatagg tgaggaggta
     8821 agtatggttc aagaaattgc acaagaaatc attcgttcag ctcggaaaaa agggacgcag
     8881 gatatttatt ttgtccctaa gttagacgcc tatgagcttc atatgagggt aggagacgag
     8941 cgctgtaaaa ttggtagcta tgattttgaa aagtttgcag ccgttatcag tcactttaag
     9001 tttgtggcgg gtatgaatgt gggagaaaaa agacgtagtc aactgggttc ctgtgattat
     9061 gcctatgacc agaagatggc gtctctacgt ttatctactg taggcgatta tcggggacat
     9121 gagagtttgg ttatccgttt gttgcacgat gaggagcagg acttgcattt ttggtttcag
     9181 gatattgaag aattaggcaa gcagtacagg caacggggac tctatctttt tgctggcccg
     9241 gttgggagtg gtaagacgac cttgatgcat gaattgtcca agtcactctt taaaggacag
     9301 caagttatgt ccatcgaaga tcctgtcgaa atcaagcagg acgacatgct tcagttgcag
     9361 ttgaacgaag caatcggcct aacctatgaa aatctaatca aactttcctt gcgtcatcga
     9421 ccagatctct tgattatcgg agaaattcgt gacagcgaga cggcgcgtgc agtggtcaga
     9481 gctagtttga caggtgcgac agtcttttca accattcacg ccaagagtat ccgaggtgtt
     9541 tatgagcgtc tgctggagtt gggtgtgagt gaagaagaat tggcagttgt tctgcaagga
     9601 gtctgctacc agagattaat cgggggagga ggaatcgttg actttgcaaa cagagattat
     9661 caagaacacc aagcagccaa gtggaatgag caaattgacc agcttcttaa agatggacat
     9721 atcacaagtc ttcaggctga gacggaaaaa attagctaca gctaagcaaa aaaatatcat
     9781 caccctattt aacaatctct tttctagcgg ttttcatctg gtggagacta tctccttttt
     9841 agataggagt gctttgttgg acaagcagtg tgtgatccag atgcgtgtgg gcttgtctca
     9901 ggggaaatca ttctcagaaa tgatggaaag tttgggatgt tcaagtgcta ttgtcactca
     9961 gttatcccta gctgaagttc atggcaatct ccacctgagt ttgggaaaga tagaagaata
    10021 tctggacaat ctggccaagg tcaagaaaaa attgattgaa gtagcgacct atcccttgat
    10081 tttgctgttt tttcttctct taatcatgct ggggctacgg aattacctgc tcccacaact
    10141 ggatagtagc aatattgcca cccgaattat cggtaatctg ccccaaattt ttctaggcat
    10201 ggcagggctt gtttccgtgc ttgccctttt agcactaact ttttataaaa gaagttctaa
    10261 gatgaatgtc ttttctatct tagcacgcct tccctttatt ggaatctttg tgcagaccta
    10321 tttgacagcc tattatgcac gtgaatgggg gaatatgatt tcacagggaa tggagttgac
    10381 gcagattttt caaatgatgc aggaacaagg ttcccagctc tttaaagaaa tcggtcaaga
    10441 tctggctcaa accctgaaaa atggccgtga attttctcag acgataggaa cctatccttt
    10501 ctttaggaag gaattgagtc tcatcataga gtatggggaa gttaagtcca agctgggtag
    10561 tgagttggaa atctatgctg aaaaaacttg ggaagccttt tttacccgag tcaaccgcac
    10621 catgaatttg gtgcagccac tggtttttat ctttgtggca ctgattatcg ttttacttta
    10681 tgcggcaatg ctcatgccca tgtatcaaaa tatggaggta aatttttaaa atgaaaaaaa
    10741 tgatgacatt cttgaaaaaa gctaaggtta aagcttttac attggtggag atgttggtgg
    10801 tcttgctgat tatcagcgtg cttttcttgc tctttgtacc taatctgacc aagcaaaaag
    10861 aagcagtcaa tgacaaagga aaagcagctg ttgttaaggt ggtggaaagc caggcagaac
    10921 tttatagctt ggaaaagaat gaagatgcta gcctaagcaa gttaaaagac gatgggcgaa
    10981 tcacggaaga acaggttaaa gcttataaag aatacaatga taaaaatgta ggagcaaatc
    11041 gtaaagtcaa tgattaaggc ctttaccatg ctggaaagtc tcttggtttt gggtcttgtg
    11101 agtatccttg ccttgggctt gtccggctct gttcagtcca cttttgcggc agtagaggaa
    11161 cagattttct ttatggaatt tgaagaactc tatcgggaaa cccaaaaacg cagtgtagcc
    11221 agtcagcaaa agactagtct gaacttagat gggcagacga ttagcaatgg cagtcaaaag
    11281 ttgccagtcc ctaaaggaat tcaggtccca tcaggccaaa gtattacatt tgaccgtgct
    11341 gggggcaatt cgtccctggc taaggttgaa tttcagacca gtaaaggagc gattcgctat
    11401 caattatatc taggaaatgg aaaaattaaa cgcattaagg aaacaaaaaa ttagggcagt
    11461 gattttactg gaagcagtag tcgctctagc tatctttgcc agcattgcga ccctcctttt
    11521 gggacaaatt caaaaaaata ggcaagagga agcaaaaatc ttgcaaaagg aagaagtctt
    11581 gagggtagct aagatggcct tgcagacggg gcaaaatcag gtaagcatca acggagttga
    11641 gattcaggta ttttctagtg aaaaaggatt ggaggtctac catggttcag aacagttgtt
    11701 ggcaatcaaa gagccataag gtcaaggctt ttaccttgtt agaatccctg cttgccctca
    11761 ttgtcatcag tgggggatta ctcctttttc aagctatgag tcagctcctc atttcagaag
    11821 ttcgctacca gcaacaaagc gagcaaaagg agtggctctt gtttgtggac caacttgagg
    11881 tagaattaga ccgttcgcag ttcgaaaaag tagaaggcaa tcgcctatac atgaagcaag
    11941 atggcaagga catcgccatc ggtaagtcaa agtcagacga ttttcgtaaa accgatgcta
    12001 gtggacgggg gtatcagcct atggtttatg gcctcaaatc agctcagatt acagaggaca
    12061 atcaactggt tcattttcgt ttccagtttc aaaaaggctt agaaagggag ttcatctatc
    12121 gtgtggaaaa agaagaaagt taaggcaggt gttctcctct atgcagtcac catggcagct
    12181 atctttagtc ttttgttgca attttacttg aaccgacaag tcgcccacta tcaagactat
    12241 gctttgaata aagaaaaatt ggttgctttt gccatggcta aacgaaccaa ggataaagcc
    12301 gagcaagaaa gtggggaaca ggtctttaat ctaggtcagg taagctatca aaacaagaaa
    12361 acaagcttgg tgacgactgt tcgtacgtct aagagccaat atgagtttct gtttccttca
    12421 gtcaaagtca aagaagagaa aagagataaa aaggaagagg tagcgaccga ttcaagcgaa
    12481 aaagtggaga agaaaaaatc agaagagaag cctgaaaaga aagagaattc ctagtcaatt
    12541 caactataat gcgttgaatc aagaatagtc cactatagtt tctagaaaat tgctggaaat
    12601 ggatgttaag ctccaattca tttgtttata tcttatttca gtttactata ctttgtgcta
    12661 aattaaagat atgaaacatg attttaacca caaagcagaa actttcgatt cccctaaaaa
    12721 tatcttcctc gcaaacttgg tatgtcaagc agccgagaaa cagattgatc ttctatcaga
    12781 caaagaaatt ttagatttcg gtggtgacac gggtctatta gccttgcccc tagcccctag
    12841 cccctagcca agcaggctaa gtcagtcact cttgtagaca tttctgagaa aatgttggag
    12901 caagctcgtt tgaaagtgga gcagcaagca atcaagaata tccagttttt ggagcaagat
    12961 ttaccgaaaa atcccttgga gaaagagttt gattgccttg ctgttagtcg ggttcttcat
    13021 catatgcctg atttggatgc ggctctctca ctgtttcatc aacatttgaa gtaagatggg
    13081 aaactcatca ttgctgattt taccaagaca gaagctaatc atcatggatt tgatttagct
    13141 gaactggaaa acaagctaat tgagcatggt ttttcatctg tgcatagtca gattctctat
    13201 agcgctgaag acttgtttca aggaaatcac tcagaattct ttttaatagt agcccaaaaa
    13261 tcactcgcct agtcagggag tgatttttct ataaggatgg aaaaaagaag ggaaatttga
    13321 taagatagga atatggattt tgaaaaaatt gaacaagctt atacctattt actagagaat
    13381 gtccaagtca tccaaagtga tttggcgacc aacttttatg acgccttggt ggagcaaaac
    13441 agcatctatc tggatggtga aactgagcta aaccaggtca aagacaacaa tcaggccctt
    13501 aagcatttag cactacgcaa agaagaatgg ctcaagacct accagtttct cttgatgaag
    13561 gctgggcaaa cagaaccctt gcaggccaat caccagttta caccggatgc tattgcttta
    13621 cttttggtgt ttattgtgga agagctgttt aaagaggagg aaattactat cctcgaaatg
    13681 ggttctggga tgggaatttt gggcgctact ttcttgacct cgcttactaa aaaggtggat
    13741 tatttgggaa tggaagtgga tgatttgctg attgatttgg cagctagcat ggcagatgta
    13801 attggtttgc aggctggctt tgtccaagga gatgccgttc gtccacaaat gctcaaagaa
    13861 agtgatgtgg tcatcagcga cttgcctgtt ggctattatc ctgatgatgc cgttgcgtcg
    13921 cgccatcaag ttgcttctag tcaagaacat acttacgccc atcacttgct catggaacaa
    13981 ggacttaagt acctcaagtc agacggatac gctatttttc tagctccgag tgatttgttg
    14041 accagtcctc aaagtaattt gttgaaagtc tggttgaaag aggaggcaag tctggttgct
    14101 atgattagtt tacctgaaaa tctctttgct aatgctaaac aatctaagac tatttttatc
    14161 ctacagaaga aaagtgaaat agcagtagag ccttttgttt atccacttgc tagcttgcaa
    14221 gatgcaagtg ttttaatgaa atttaaagaa aattttcaaa aatggactca aggtactgaa
    14281 atataaaata gattttgtta taatagttga aaacgcttaa aaaggggtat catgttatga
    14341 caaaaacaat tgcaatcaat gcaggaagtt caagtttgaa atggcaatta tacttaatgc
    14401 cagaagaaaa agtattggcg aaaggtttga ttgaacgtat cggtttgaaa gattcaattt
    14461 caactgtaaa atttgacggc cgttctgaac aacaaatttt ggatattgaa aatcatacac
    14521 aagctgttaa aattttattg gatgacttga ttcgtttcga tattatcaag gcttatgacg
    14581 agattacagg tgttggacat cgtgttgttg ctggtggaga atatttcaaa gaatcaacag
    14641 ttgttgaggg agatgtttta gaaaaagttg aagagttgag tttgttggct cctctacaca
    14701 acccggcaaa tgcagcaggt gttcgtgcct tcaaggaatt gttgccagac attaccagtg
    14761 tagttgtttt tgatacttcc ttccacacaa gtatgccaga gaaagcttat cgctaccctc
    14821 taccaacaaa atattacaca gaaaacaagg ttcgtaaata cggtgctcat ggtacaagtc
    14881 accagtttgt agcaggagaa gctgcaaaac tcttgggacg tccattagaa gacttgaagt
    14941 taattacctg tcatattggt aacggaggct caattacagc tgtgaaagcc ggcaaatctg
    15001 tagacacttc tatggggttc actcctcttg gtggtattat gatgggaacg cgtacagggg
    15061 atattgatcc agctatcatt ccttatttaa tgcaatatac agaggatttt aacacaccag
    15121 aagatatcag tcgtgttctt aaccgtgaat caggtctttt gggagtttct gctaattcta
    15181 gcgatatgcg cgatatagaa gcagctgtag cagaagggaa tcacgaggct agcttggctt
    15241 atgaaatgta tgttgaccgt atccaaaaac atatcggtca gtaccttgca gtgctaaatg
    15301 gagcagatgc cattgttttc acagcaggtg tcggtgaaaa tgcagagagt ttccgtcgtg
    15361 atgtaatctc agggatttcg tggtttggtt gtgatgttga tgatgaaaag aatgtctttg
    15421 gcgttacagg agacatctca acagaggcag ctaaaatccg tgtcttggtt attccaacag
    15481 atgaagaatt agtcattgcc cgtgacgttg aacgcttgaa aaaataagtg aaactaaaaa
    15541 aatattcaat acaaggagtt gggaaagtta tttttccagc ttctttttct gatgaaattg
    15601 tccaaaacct tgctatgatt ggcttttttg aaaaatatgg tataatagta gtaatttaat
    15661 agatggagtt gagttttgaa gaaaaacttt cgtgtaaaaa gagagaaaga ttttaaggcg
    15721 attttcaagg aggggacaag ttttgctaat cgcaaatttg tggtctacca attagaaaac
    15781 cagaaaaacc attttcgagt aggtctatca gttagcaaaa aactggggaa tgccgtcact
    15841 agaaatcaaa ttaagcgacg gattcggcat attatccaga atgcaaaagg gagtctggta
    15901 gaagatgtcg actttgttgt cattgctcga aaaggagtcg aaaccttggg atacgcagag
    15961 atggagaaaa atctactcca tgtattaaaa ttatcaaaga tttaccggga aggaaatggg
    16021 agtgaaaaag aaactaaagt tgactagttt gctaggactg tctctgttaa tcatgacagc
    16081 ctgtgcgact aatggggtaa ctagcgatat tacagccgaa tcggctgatt tttggagtaa
    16141 attggtttac ttctttgcgg aaatcattcg ctttttatcg tttgatatta gtatcggagt
    16201 ggggattatt ctctttacgg tcttgattcg tacagtcctc ttgccagtct ttcaggtgca
    16261 aatggtggct tctaggaaaa tgcaggaagc tcagccacgc attaaggcgc ttcgagaaca
    16321 atatccaggt cgagatatgg aaagcagaac caaactagag caggaaatgc gtaaagtatt
    16381 taaagaaatg ggtgtcagac agtcagactc tctttggccg attttgattc agatgccggt
    16441 tattttggcc ctgttccaag ccctatcaag agttgacttt ttaaagacag gtcatttctt
    16501 atggattaac cttggtagtg tggatacaac ccttgttctt ccgattttag cagcagtatt
    16561 caccttttta agtacttggt tgtccaacaa agctttgtct gagcgaaatg gcgctacgac
    16621 tgcgatgatg tatgggattc cagtcttgat ttttatcttt gcagtttatg cgccaggtgg
    16681 agtcgcccta tactggacag tgtctaatgc ttatcaagtc ttgcaaacct atttcttgaa
    16741 taatccattc aagattatcg cagagcgcga ggccgtagta caggcacaaa aagatttgga
    16801 aaatagaaaa agaaaagcca agaaaaaggc tcagaaaacg aaataaataa ggaggaatct
    16861 ggtaatggta gtatttacag gttcaactgt tgaagaagca atccagaaag gattgaaaga
    16921 attagatatt ccaagaatga aggctcatat caaagtcatt tctagggaga aaaaaggctt
    16981 tcttggtcta tttggtaaaa aaccagccca agtggatatt gaagcgatta gtgaaacgac
    17041 tgttgtcaaa gcaaatcaac aggtagtaaa aggcgttccg aaaaaaatca atgatttgaa
    17101 cgagcctgtg aagacggtta gtgaagaaac cgttgacctt ggtcatgtgg ttgatgctat
    17161 taaaaaaata gaggaagaag gtcaaggtat ttctgatgaa gtcaaggctg aaatcttaaa
    17221 acatgaaaga catgccagca ctatcttaga agaaactggt cacattgaga ttttaaatga
    17281 acttcaaatc gaggaagcga tgagggaaga agcaggcgct gatgaccttg aaactgagca
    17341 agatcaaact gaaaatcaag acttgaaaga gatgggcttg aaggtcgagc aaagttatga
    17401 tattgcccag gtggctacgg atgtgactgc ctatgttcaa gcgattgtgg atgacatgga
    17461 tgttgaagct acactttcaa atgattataa ccgtcgtagc atcaatctac aaattgacac
    17521 caacgaacca ggtcgtatta tcggctacca tggtaaagtc ttgaaggcct tgcaactgtt
    17581 ggctcaaaat tatctttaca accgctattc cagaaccttc tacgttacaa tcaatgtcaa
    17641 tgattatgtc gaacaccgtg cagaagtctt gcagacctat gcgcaaaaat tggcgactcg
    17701 tgttttggaa gaagggcgca gtcataaaac agatccaatg tcaaatagcg aacgcaagat
    17761 tatccatcgt attatttcac gtatggatgg cgtgactagt tactctgaag gtgatgagcc
    17821 aaatcgctat gttgttgtag atacagaata agtaaaatca ggtttatcct gattttttgc
    17881 tagttagagg aggttaaact gatgttgaat aagataagag actatt
//
LOCUS       DAVBDG010000040        16769 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBDG010000040 DAVBDG010000000
VERSION     DAVBDG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.041"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(821..979)
                     /locus_tag="VQ526_001394"
     CDS             complement(821..979)
                     /locus_tag="VQ526_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555108.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(989..2299)
                     /gene="obgE"
                     /locus_tag="VQ526_001395"
     CDS             complement(989..2299)
                     /gene="obgE"
                     /locus_tag="VQ526_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2555109.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEETYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            complement(2363..2497)
                     /locus_tag="VQ526_001396"
     CDS             complement(2363..2497)
                     /locus_tag="VQ526_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2555110.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            complement(2556..2732)
                     /locus_tag="VQ526_001397"
     CDS             complement(2556..2732)
                     /locus_tag="VQ526_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2555111.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            complement(2763..4088)
                     /locus_tag="VQ526_001398"
     CDS             complement(2763..4088)
                     /locus_tag="VQ526_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2555112.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAISEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            complement(4104..5147)
                     /gene="cpoA"
                     /locus_tag="VQ526_001399"
     CDS             complement(4104..5147)
                     /gene="cpoA"
                     /locus_tag="VQ526_001399"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2555113.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPATLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            complement(5262..5591)
                     /locus_tag="VQ526_001400"
     CDS             complement(5262..5591)
                     /locus_tag="VQ526_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2555114.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            complement(5605..6714)
                     /gene="rpoD"
                     /locus_tag="VQ526_001401"
     CDS             complement(5605..6714)
                     /gene="rpoD"
                     /locus_tag="VQ526_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2555115.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            complement(6717..8477)
                     /gene="dnaG"
                     /locus_tag="VQ526_001402"
     CDS             complement(6717..8477)
                     /gene="dnaG"
                     /locus_tag="VQ526_001402"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2555116.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKIIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRVGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VKEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            complement(8567..9325)
                     /locus_tag="VQ526_001403"
     CDS             complement(8567..9325)
                     /locus_tag="VQ526_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555117.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQVTIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            complement(9322..10188)
                     /locus_tag="VQ526_001404"
     CDS             complement(9322..10188)
                     /locus_tag="VQ526_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555118.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            complement(10257..11261)
                     /locus_tag="VQ526_001405"
     CDS             complement(10257..11261)
                     /locus_tag="VQ526_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2555119.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(11374..11591)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11674..14370)
                     /gene="ppc"
                     /locus_tag="VQ526_001406"
     CDS             complement(11674..14370)
                     /gene="ppc"
                     /locus_tag="VQ526_001406"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2555120.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(14390..15619)
                     /locus_tag="VQ526_001407"
     CDS             complement(14390..15619)
                     /locus_tag="VQ526_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2555121.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(15775..16077)
                     /locus_tag="VQ526_001408"
                     /pseudo
     CDS             complement(15775..16077)
                     /locus_tag="VQ526_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(16525..16746)
                     /locus_tag="VQ526_001409"
     CDS             complement(16525..16746)
                     /locus_tag="VQ526_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2555122.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTDSAYAKIERGEHTLTADI
                     LVTLSNFYDVSTDYLLGLTDFPDKIRFRK"
BASE COUNT         4949 a         3746 c         2918 g         5156 t
ORIGIN      
        1 atcctacacg tacggcaagg cgatattgac gtgatttgaa gagattttcg aagagtataa
       61 tctttctcga atcgctattg cccctttttt atcccctgaa accgagtagg agctaagact
      121 agatctctta tctaagtatc ggaatacggc tgttcaacta aattttagca agaacttctc
      181 ccccttctct cagttcacag aagcgtttta tacataccgt cctatcagac aatctatttt
      241 aaagataacc gtgactcagg ctcacttcta tctgtcgttt tctctttata gtgatagacc
      301 tcacgggata agtcgtacat ctttcctcgt gtatacaaga tataaggacg ttaaaaggtt
      361 acagtgaaaa tagaaaatct tagtacgaat tcaattactc tcgtgattta cgcatatagg
      421 ttacgactac ctttttggac tttagagttt gaggccctct tatccgctat atacgcctta
      481 ttatcacgtt cctgttcgta gagccacgat ttcgctatcc cttcttctga caattcttat
      541 ttccagcgtc agacttaaaa cggtctatcc ccagaccatt ttaatccgct acctcacgat
      601 agtcaggctt gggaatcgct atttgactcg ccgagaactt tgacatcaga tatactataa
      661 caaattgaaa taagaatcaa acaatttaat tttgaaattc aaatcaattt ataacaatat
      721 tttagaagat atgctgtgct attccagatt cagtttgcta tacatgcctg tcgtaccaca
      781 aaatcccgtg tctgatgagg cacgggattt tttagcttct ttactgagat ggattttcct
      841 ttttagttcg tgccaatctc aaggcacgat ttggattgag acgattaaat agttcttcca
      901 atttcttttc attccaaacg tctgcggcgg aaacaaaatt accatccgca tctcggaaag
      961 atatcggttt atctcccata ccagtctcct agtctacaaa ttcaaactca aatttaccaa
     1021 tgcggaccaa atccccatct ttagctccac gcgcacgaag ggcttcatca acccccatac
     1081 cacgaagctg acgggcaaat ttcatgacag attcatcacg atcaaagttg gtcatattaa
     1141 agagtttcat gagtttttca ccagaaagta cccatgtcgc atcgtcatca cgactaattt
     1201 caaaggcttt ttcttcttcg tcaaatccat agtaagtttc ttcttccata tcggactcgt
     1261 cgtagagcaa aaattctggt gtcttgtcta acaattcagc tgtagcatct aaaagtgttg
     1321 ccagaccttg cttggtcaat ccagaaattg ggaagatagc tggtaactct tcaaattcat
     1381 cataattttc agccaatttt ttcttaaatt cttcaagatt ttcctgactc tcaggcatgt
     1441 ccatcttatt agctacaata atctgtggac gctccatgag gcgaagattg taagactcca
     1501 gctctttatt gatagctaag taatcctcat atggatcacg gccttcgcta gctgacatat
     1561 caatgatgtg aaggataaca cgtgtacgct cgatgtgacg gaggaactga gttcccaaac
     1621 caacaccttg actagcccct tcaatcaaac ctggcaagtc ggctactgca aaggattcac
     1681 ctgattgggt gcgaaccata cctaaatttg gtacaatagt ggtaaagtgg taggcaccaa
     1741 ttttaggctt agctgaggta ataacactta aaagtgttga cttccctaca gatgggaatc
     1801 ctactaaacc gacatctgcc aagattttta gttccaattg taactcacgt tcctgacctg
     1861 gttctccatt ttcagagatt tccggtgcag gattttttgg tgtcgcgaaa cgaatatttc
     1921 cacgtccacc acgaccaccg tgggcaacga taaattcttg cccatgttca atcaaatctg
     1981 ttaaaacctt gccagtctcc gcatcacgaa cagtcgtacc ttgtggtact cgaactctaa
     2041 ggtcctcagc accacgacca tgcatccctt tggtcatccc tttttcacca gaatcagcct
     2101 tgaaatgacg attgtagcga aaatccatca aggtacgtag tccttcgtct acaacgaaga
     2161 ccacattgcc tccacgacca ccatcaccac cccaagggcc tccattaggg acatattttt
     2221 cacgacgaaa ggcaaccata ccatcgccac cattaccagc cttgacctta atcttagctg
     2281 tatctaaaaa catactcatt atttcttctc actttaaaaa agggctggga aatcccagtc
     2341 actaaatttt cttgaatcta ttttatagat tactgagggc accaattgca gttgcaaaaa
     2401 ttcccaataa acttgctact agcatgataa tcacgataaa caaggttatt ttctcaaaca
     2461 tagttttttt acgatttcca ttatctccaa atgccatttt tctctccttc gttacattct
     2521 attttctatt atcttagcat gaattgagct agttttcaat agtcagttgc tactggtgcg
     2581 ataatccata aaataatgta agctacaatt ccagctccgt aacagcaagt aagaacaccc
     2641 caaatgactt gaacgatagt cggatccata tccagataat gggccacccc agcacaaaca
     2701 cccgcaatct ttttatcacg accgcttctc attaattgtt tttttcatag ctgttcctct
     2761 ttctattctt catggtcttt ccaatagtct ctcatactga tcaactgtgt ttttgaagcc
     2821 ttcaacatgc gtctagatcc ttttacaggt acagcaacca cctgttgcgg aagatacaaa
     2881 actgtcttaa agatacgctg actgaccgta tcatctttag ccaaatcttt ctggaagtta
     2941 tttgaaataa tggcatccag ataaaactca tgcactcgtt tcccaaagtt ctcagctgaa
     3001 atctcataca atttctctga taaggtatgc tcattcatgt ctggtgttgc aatcagggct
     3061 tccgaaatag caccagccaa atcatgttct ccatagtaca aggttccaaa cattttatca
     3121 ctgatgaggt tgttcaaata aggatttccg tgagcaatga caggtgttcc actggctaag
     3181 ctttccaagt aggtcaaacc ttgcgtttcg cttgtcgatg ccgaaatgaa gaaatccgcc
     3241 gctttatagt aaagagccgt ctcactagga gcaatcatcc ctgtaaagat gactgagtct
     3301 tgaatctcta ggttctgggc ttgctctttg aggtcattca gataagggcc atccccagct
     3361 actaccagtt taaccttgtc ttcctctttc agaacatcag caaaggctac taaaactgct
     3421 tgaatatttt tttcatagga gattctcgaa agactaagca acgtcttttc accatcttga
     3481 atccctagtt tactacgcag ttctttcaaa ttttcctgct tgatttccgg acgctcaaac
     3541 ttggctaatt caattccagt aggaatgacc cgtttttcaa ccttgacctt ataatcagat
     3601 agcaagtcac ggacaatctc actagggcaa ataaccccat ccacatcatg caggaaacct
     3661 ctaaccagat acttgaccat actcggccgg atcaacatcc ccttagcaat ataatggaca
     3721 tagtcttcat actgggtgtg ataggtatgg atgactggaa ttttcaattc acgcgcaatc
     3781 caaatcccca acaggccaag agaaaattct gtctgagtat ggataatatc tagctgatac
     3841 tgtttagcaa tttcaagtgc cttgctaaaa cctcggtagg caaagcgacg atccttaaaa
     3901 gcaaagaaag gaacacttgg aatgcggata atttgccaat cttcgtagcg attgacatcc
     3961 ttatctgtcg tcgtaaagat aaaaacagca tgtccctgct tttcaagttc tgttttcaag
     4021 gttcgaatac tggtcgcaac accagaaacc tgaggaaaat aggtatctgt aaataaacca
     4081 attcgcatag attacctcac tttttacttt ctccctaaag cggcttgttt ctcataaaag
     4141 tccaaccaga tttgtaacag atgctcttca gaatactctc tggaaatatt cttagccttt
     4201 tctttgagat cttttaaggt agcaggattt gcttgatatt ccaaaatagc ctctttcatc
     4261 tcttctctac ccgctgtcgc ccgataattt ccctccaaaa tcaccttata gagatctaaa
     4321 tcacgcaaca taataggagc ctcacaactc gcagcttcta aaatagtcat aggaaagagc
     4381 tcattgtaac taggcaacaa gaaaagatcc gctagagcat acaattcgcg catccgctct
     4441 ggcgatacaa tgcctggaaa aatcaaattt ttagggggat tttccataat tttcttatag
     4501 tgttcataac catctgtcat accaccaaaa gagaagccac cagcccagat aaaggtaatc
     4561 tgaggcaatt cctcagccag acggataaag tcatcaatcc ctttacgttt ctgaacttgc
     4621 ccagcaccta ctacgataaa ctgattgtca ctaagaccaa gatctgtgcg cagtctaact
     4681 acctcttctt gtggtagagg atgccatttt tccttgttga caaagttagg aatataggtc
     4741 actttttcac gtggaatacc agctgctacc aaatcctcaa taaacatagg attgaccaca
     4801 accaagtgct ccatccggtt gtaaaaagaa aatacatagc gtttcacaat tccctttaag
     4861 aaaaatggaa ttttcaaact tccctcaagt gtagctggca agaaatgcac atagccaatc
     4921 tttctccctg agcgtttctt ttggaaggtt gataaataat agggaaaatc aatcgtatga
     4981 aagtgagtca catctgcctc gattggaaga ttttctgtaa caatcaattg gtccttggca
     5041 gcacggtgaa gaagacgaac taattcacgg taagcacctg aaactccctg tcctgctact
     5101 ttctcacttg aactcaacat attgatgcgt aatttctttt tctccataac tactattata
     5161 tcattttctt tgaataaaat cagcaaaaga aaggagagac tagaggaaaa gaggggaaat
     5221 ttcctcgctt ttccaacagt ctctcacatg cttttatatg tttacttaat gcctagggca
     5281 atgcgggcat agcgactcat tttttcaact gtccaggctg gataccagac taatttaacc
     5341 tcagtatccg ttacttctgg aacctctatc atagcatcat aaatctggtc tgtcaaaagg
     5401 tctgctaagg gacaacccat agttgtcaaa gtcatgtcaa tctctgtttg ccctgtgtca
     5461 ccgtcaaaac gaatctcata gatcaaacca agattgacaa tatcgattcc caactcaggg
     5521 tcgatgactt cttccaaggc tgttaaaatc cgtgttttga tgttttcaat ttgctcttct
     5581 gtataagcca tattttcctc actcttagtc ttcaataaaa tcacgaagcg gtttgctacg
     5641 acttggttgg cgtagttttc tcaaagcctt tgcttcaatc tgacggatac gctcacgagt
     5701 tacgttaaag actttcccca catcttcaag tgtgcgcatt tttccatcat ctagtccaaa
     5761 acgtagacgc agaacatttt cttcacggtc tgtaagagta tctaagattt catccaattg
     5821 ctcacgcaag acgatacgag tcgtataatc cactggattt tcaatcactt catcttcgat
     5881 aaagtctcca aggtggctat cgtcctcttc accgatagga gtttcaagag atactggttc
     5941 ttgggcaatc ttcaagattt cacgaacctt atcaggtgtc atatccattc gttcagcaat
     6001 ctgttctggt gtcggatctt gccccaattc ttgaaggaga ttccgctgtt cacgaaccaa
     6061 tttattgata gtttcaacca tgtgaactgg gatacggatg gtacgagctt ggtccgcaat
     6121 agcacgagtg atagcctgac gaatccacca agttgcataa gttgaaaact tgaacccttt
     6181 agaatagtca aacttgtcaa ccgccttcat caagcccata tttccttctt gaatcaagtc
     6241 aaggaactgc ataccacgac cgacatagcg tttggcaatg gaaacaacca aacgaagatt
     6301 ggcttccgca agacgttgtt tggcttcgat atcaccagct tcaacagcca gtgccaactc
     6361 tttctcctct tcattggtca agagaggaac gacccctatt tctttcaagt acatacggac
     6421 agggtcattg accttagcag aagttgaccc aatcaagtcc tcatcgctga gttctggttc
     6481 ttcttcattg ctaagaacac gcgcacttgg atttccttcg ttatctgtga tagaaattcc
     6541 tgcatcctga atccgttgca agagatcttc aatcccatca gcgtccaagg taaaaggaat
     6601 aaccaaactt gcattgattt catcatctgt tgctgtccct ttttgcttat gattacggat
     6661 aaattctgct acctgtacgt caaatgttgt tacttctttt tgttttgttg ccattattac
     6721 tccattcttc tcttttggga aattaaacgt tccaattctt ctagggctgt atctgtatct
     6781 cctacatggc tagcttcctg caccttcttt ttgattctca tattgtcctg attcaagaga
     6841 gccttgtttc gagtcatctc tacttcacta agttcctgcg gcgatatctc agcaggcaaa
     6901 tcctgagcta aaacttggta ccaagctctt tcaacttcct ctgtctgctc tgctaaaact
     6961 tctggaggaa gatttccata ctggccaagc aagtcatata agacctgaaa ttcaggtgta
     7021 gcaaatgcaa agtcttctcg caaacggtaa tcgttcaaaa caagagggga ttccatcatc
     7081 cgatagagta gatgggcttc tgccctcata atagccgata actgcttggt gacaggcatg
     7141 gtgattggcg tcggtctgga aattctttcc atgcgattct gcctttgcac ctgacgactc
     7201 tcattaacaa tctgctcaat ctgggcataa tcaaaggacg ccagactgtc agctaaaata
     7261 tgaatatagc tgttttgagc agcgatggac ttttctttaa caatcaaggg agctattttt
     7321 tcaataaact caatctgagc ctgcagattt tcactgtttt caggtttgta ctgatgaatg
     7381 tagaactcaa tcggactaat acgagttttc gttaatagat aggccaagtc ttctggacca
     7441 tttttttgta gatactcatc aggatccaag ttatcaggca tgctgacgat ttgcacaggc
     7501 atatcaccaa tttcatccaa tgctttcaat atcgcggctt gcccagcctt atctccatcg
     7561 taaacaagaa ccaatttctt ggttaacctt ttcagatgct caacatgctc tcgactcaag
     7621 gccgttccca tcgacgccac agcattttcg attccaaccc gataggctgc aataacatcc
     7681 atgaatcctt ccatcaggta aatctcacta gcttttccag aagatttttt tgccctatcc
     7741 atatgatata attcgtaact tttgttaaaa attgcagtcg atcggctgtt tttatactta
     7801 gaagtttgtg aatccgtttt ttgccagata cgacctgaga aggcaatgat ctttccttgg
     7861 tcatttgtca ggggaaacat aatgcgattg tgaaaggtgt ctacaaattg attggcatcc
     7921 gagagataaa acaggcctga atccagtaaa tcctcttcac gatactgatc agacaaacgt
     7981 tgatagagat agtttcgttc tggaggtgct aaaccaatcc aaaaatgttt aagcacttca
     8041 tctgtcaaac cccgctgata aaggtaattt ctggcctctt ctcccatagt cgttgtcatg
     8101 agaatagcat ggtaaaattt ggctgcatct tcgtgcatat cataaagagc ttggtgaggt
     8161 gaggctgact tctgctcact ataaagcggt ttttcaacct caattccaac acgctgacct
     8221 aagatttgga ctgcctctat aaagggaacc ccttggtact cctcgatgaa cttaaagaca
     8281 tcacctgagc gaccacaacc gaaacagtga taaaactgct tatcctctac aacgttgaaa
     8341 gatggtgttt tttcgccatg aaaaggacag agccctagat agttccgtcc tgccttttgt
     8401 aaagaaatca catctcctat gacttccaca atgttggcat tgtttttgat ttcttcaatg
     8461 acttgtttgt caaccataca caatacctcc atgttatcat agtatactat atatagtata
     8521 ctttatttca gaaaaaaagt atactatttc actcattttc cctactttat tcaaagagtt
     8581 gataataatc agagattttc atttttgctt tttcttcttg gtttaaatct tggataatcc
     8641 gtccttcttt catgacaatc aagcgattgc cgtatttgag agcatcttcc atatgatgag
     8701 taatcataag ggctgttagc tgatctttct taacaaattc atctgtcaat tccatcaaag
     8761 caacactagt ctttggatcc agggcagcag tatgctcatc taacaggagt aattcaggtc
     8821 gcttcaaggt tgccatcaag agactcaaag cctgtctttg tccacctgat aagaactcaa
     8881 tcggtgtatt caagtgtttc tcaagaccat ttcctacttt ttcaatggtt acctgaaatt
     8941 catccttata gctagtcaag cgtcgtggta acaatccacg cttttcacca cgaaacttgg
     9001 cgattaaaag attttcagcg accgtcatac ggggagctgt ccccatcttt ggatcttgga
     9061 agacacgaga caggtacttg gcacgcttct cgggtgaaaa cttagtgaga tcttcaccta
     9121 aaatacggat agttccacta gttagtgata aggtccctgc tatagtgtta aagagggttg
     9181 attttccagc accatttccg cccaaaatcg tgataaagtc ccgttcaaaa atttctaagg
     9241 aaacatcatt taaaataatc ttttcttcat caaagccatt tttaacgatt ttggttgcat
     9301 tttttaattc tacaattgct gtcatttgct taacttggct cctttcaaga ttgtttgctt
     9361 aaatgttgga atcatgaggc agactgctaa aatcaaggca ctgtataaac gaaggtaact
     9421 tgtattaaag ccaagtgcga taactgccca cactaaaaat tgataagcga tagaacctac
     9481 aacgatagta accaaacgct ctgccaagct caaactcttg aaaataactt ctccaataat
     9541 caaacttgca agccccacaa cgataacccc gatccctcga gacacatcgg cataaccttc
     9601 ttgctgagca atgagggcac ctgcaagggc aatcacacca tttgataaga ccaagcccat
     9661 gagctccatg cgtccagtat gaatcccgaa acttctagcc atatcaggat tatcccctgt
     9721 agcaatatag gcttgtccga gtttagtgtc caagaaaaag agcatgagag caataacaat
     9781 actcacaaag atgagacctg tcaagagttg attcaaatcc gaatcaaaag gcaaaacatc
     9841 ctgaatttgc ttggttccaa gcaggcctaa attcgcacgt cccataatca agagcatgat
     9901 tgagtgacaa gaagtcatca ccaaaatccc tgagagcaag gttgggatct tcccttttgt
     9961 ataaagaagg cctgctgcca ttccagccaa acaacctgct cctacagcaa caagtgtcgc
    10021 taaaaatggg ttcacgcctt tggttatcaa agtgacagca acagctcccc caagagggaa
    10081 ggaaccttct gtcgtcatat ctggaaagtt taaaatccta aatgtcataa agattcccag
    10141 acctagaata gcccagacaa atccttgaga aataatggaa acaatcatat tttatttaat
    10201 cctttctata ttcatctttt taaaaaatgg gaagagtctc ctcctcccta ccttatttat
    10261 tcgatgactt gtcctgcttc tttgagaaca gactcaggga tagtaatacc tagttcttgt
    10321 gctatttttt tattgatgac tgacttacca gttgaaaaga cattgactgg ggtatcggct
    10381 ggttttgcac ctttcaagac ttgcacaatc attttacctg ttgccacacc aaggtcatgt
    10441 tggtcaatta caactgatgc caaaccacct acttctacca tagctgtcgc actgggataa
    10501 attggtttct tagaactttg attgctagag acaaccgttg gaaatcctga tgcaatggtg
    10561 ttatcaattg gaacccaaat agcatctacc ttgctagtca taacagtgac agttgaggca
    10621 atttcatttg ttgaaggaac tgcaaatgtt tccactgtca gacctgcttt ttcagcataa
    10681 gccttaaatt cttcgacctg tgtttttgaa ttgtcttcgc tacttgagta aagagctccg
    10741 attgttttca cattcggtgt cagagccttg atgagttcaa cttgttgttg agctggattg
    10801 tggtcagata cccctgtaat gttgccacct ggttttttca aatctttaac caagttagca
    10861 ccaattgggt ctgtaatagc ggccatgata accggtaggt cttttgttgc actagccaac
    10921 ccttgggctg ctggtgttgc gataccaacc acaaggtcat tcccatttgc aaccaattgt
    10981 ttactcattg tcgcaacctt actttggtca ccttctgagt tcataaaatc aattttaact
    11041 tgatcatctt tatatccttc ttctgcaagt ccatcttgga tccctttata aatcaaatca
    11101 agggatggat ggctcacaaa ttgaagcaca ccaatcttag ttattttctt ctcatcctta
    11161 ttattctgag cttctgattt attcattgaa gaataaatca agcttcctgc tactaagact
    11221 gctaatgcag caataattcc aattaaacgt ttatttttca ttctgtttct cctttttatt
    11281 tcctttaaca tatttcactt atctaaacaa gcgacgccat cgttttcttt ccatactaac
    11341 ctccatcaaa aaaatcctca cacaaaaaac ttgtgtgagg acgtcgatgc gcggtaccac
    11401 ctcaattata gggaatttcc ctatcgctca gtctcgcaat aacgagatgc actgtaaggt
    11461 gtgcttaccg aatttttata atttcaaatt ctaaatagca ttcagcccaa ttttcatcat
    11521 cgccacttat ctgttttcag ctaccacaga ctctctaaaa agtttgtatg attacttttc
    11581 tgaatggtta aattatatca tttttaaact acttgtcaat agtctattag aatttttttg
    11641 atattcactt ttagcattca ctcttgaaaa ttatcaacct gaattacgca atcctgtcgc
    11701 aattccgttg atggtgatat gaatcaatcg ttcttgatca ctggacaatt ctccacgacg
    11761 ttggcgttta atcaactcta actgaatata gttgagaata ttaaagtaag gcatacggta
    11821 atccagacta gcttttagat atggattgtc agctaagagt tcgtcatgtc cttcaatagc
    11881 caagataacg ttcttagtaa cttgccattc atttaaaata atctcataga tggccttaac
    11941 ttgctcgtct tcacaaagtt tagcatattc aaaagcaata ttcatatttg attttgacaa
    12001 aaccatatca acatttgaaa gaagcgattg gaagaaaggc caattttggt acatatctcg
    12061 taagatagca atattctctg gatttttatt gataaattcc ttgaagcttg aaccaacccc
    12121 gtaccatcca gggaacataa cacgactctg tgaccatgag aatacccaag ggatggcacg
    12181 caaaccaccg atttcagtaa tagtcttacg agcggctgga cgagaaccaa tattaaaact
    12241 tgaaatagcc ttgattggac ttgactcgaa gaaataatca tagaaatgct cattaccaaa
    12301 gaccaaatca cggtagatat cgtaactacg gtccactact tgatccataa tggcttcata
    12361 acgatttggg gtattggtat cgctcttctt ctgagtaatc atacggttaa tagctgccga
    12421 tactagcatt tcaaggttat agtaagcggc gtctttgtta ccgtatttat tcccaattac
    12481 ttcaccctgc tccgtcaaac ggatacgatc cttgatagac ttgagcggtt gagatgtaat
    12541 ggcttcatag gttggcccac caccacgacc gacagtacca ccacgaccat ggaagaaggt
    12601 aaccttaacg ccaaattcat ctccaatagc agtcaattgt tgttgagcct tgtagagggt
    12661 ccaacatgat gacaagtaac cgccatcttt attactgtca gagtagccaa gcatgatttc
    12721 ttggtagtta tttcgtgagt caatccattt tttggcaagg ctaagagaaa gatattttct
    12781 cattgtttcc tctgaatgat ccaagtcttc aattgtttca aaaaggggaa caatctgaac
    12841 acgcgccctt tccgtatcca ccagtcctac ttcttttaac agaatagcta attctagcat
    12901 atcagaaagg ttggttgcat gtgaaatgat ggtctgacgg atgacatcat ctcccaactt
    12961 atctttcaaa acacgagccg tcttaaaaat agctaattct tttgctaata attctgattt
    13021 ttctgcgtga gtcgcagaaa gaattcgggg atcttcttct aattctttca agagaaggtc
    13081 acacttttct tcttcgctca actcgctata acgagaatga attcctgctg atttcaagag
    13141 ttctgccaca caggcttcat agacgctaga gtcttgtcgc atatcaattg atgctaagta
    13201 aaaaccaaat atctctactg cctgcaataa ttccacaaaa tcacctgaaa tcaaggactc
    13261 gcccttattt tctagtagag aatctcgaat ggcaatcaaa tccttgtaga aatcattggc
    13321 ggtttcataa cgagtcccaa cttcttcatc ctcaatcaga taggttttag ttgcctgaat
    13381 ttttgattga atatcaaaca aggcacgacg gtaaagctct ttttcgcggt aaatcgagtt
    13441 atccttggat tgacgagcca tttctctgac ttgcttgctg acgttgacaa tgctagttga
    13501 aagagaaaat tcacgataaa gttggtaaat ctttttatca tagtagttca tgatgacttc
    13561 acactgagtg agtgcagact gcttcaaggt ctttgctgta acaaatggat ttccatcacg
    13621 gtcaccacct atccacatac ccatggtgat tggtttagcc tgttttaaat tcagaccatg
    13681 cgcttgcgct aagcgcttat actccgtcgt caaatgaggt acagctttca aaaaggagct
    13741 gttataatat tccatagcat tcgtgatttc gttagtcact tttaattttt tctcacgaat
    13801 catgtctgtc tgcatgataa tttcgatgta acgacgcaaa tcattgtacc atttatcttt
    13861 attgatcaac cccaacttaa catcacggta tttacgcaaa agactatgaa tatgatttgt
    13921 taaatccaac atacttttgc gttgcacttg tgttggatgg gctgtcaaaa cagggacaac
    13981 attcaagtgt tctaggatct caacggcatt ttccttttct gctaccaatt taatcgttgt
    14041 agataattta cctaaatagt cctgatcaat attattttga tgattgattt cataagctaa
    14101 atccacatcc tctgaaatat taatcaaaag aggcaagata gagaaatagc gtgaaatata
    14161 gaccatttca tcatttgata agctagtcac tagacggttt agaccttgat aatcttcctg
    14221 cgttgataat tctttcaact gtattatttt ttcaaaggtc tctggggcaa gcatattttt
    14281 agtaatatct tccagtaatt ctgttagaat caagacttct tcttgcacaa cacttttatt
    14341 actataattt tctaattttt gaagagacat aaactatcct ttctttatcc tacttcaaca
    14401 gaaggttcat tggttgattt tccaattctc ggtacaattt agcgcgtttt tcactggcat
    14461 caatatttaa gacaaaggct actgccactg ataagactag aagactattt ccaccctggg
    14521 ataagaaggg gaaagtcact cctgtagatg gaatcaagcc cgaaatccct ccgatattga
    14581 caaatacctg aaccaacatc atccctccga caccgagtgc aaccatggca ttgaaaggat
    14641 tctccgctcg gataccgacc aagataatcc gcaaaatcat gaaaaacaag agagctaaaa
    14701 taagactggc accaacaaag ccaaattctt caatcacgat agaaaagaca aagtctgtat
    14761 gagcttctgg caaataacct cgtttttcaa tcgagtttcc aagacctaga ccaaaccaac
    14821 cgccattgac catggcaaaa taagaattag ctaactggtg acctgcatca gcacgatcgg
    14881 caaaaggatt aaaaaaggca ctaaagcgct tggctacata gccaaatact ggaatttttg
    14941 aaaaggtctc aacaccgatt aggctgatag tggtcaagac aaagacagaa gtggcagata
    15001 cgagcgccag aatggttgaa aaccagcgat aagcgattcc actaactgta tacataatca
    15061 aggaaaccaa gactaaaata gtcgcatttc ctaaatcagg gaaaattccc aaacttccaa
    15121 tcagaactag gagaacgaat cgccaatcat taaaagcacg gggaagccat tgattttgag
    15181 tcaaaacttg aaaatcataa gtagctattt cttcttgctg tttggagaat cggtgagcta
    15241 aataccaaat aataatgatt tttaagtact cagctggctg aatagttact cctgcaaccg
    15301 aaatccaacc gtatgccccg tttactgaaa taccaataaa acgagccaag aacaataaaa
    15361 gcatttctat taatataact aaaatgatta gtcgctcatt tctcaaaaaa tctagtctca
    15421 atttataaat taaggcaatc agtatcaaac taacaatcca aaagattcct tggtttcgaa
    15481 ccaactgcaa ggcgctcttg ccttcttcaa ttaaaatagc actggtggtc gaatagacca
    15541 caatcaagcc caaaatagat aaaagcaagt agggaatcaa gatggaataa tttaataagt
    15601 gcctcttact aatcttcata gtatcaccac tctactagga tacagacaaa tctgttccaa
    15661 attccgtttt attttctagt tttgattgct attataccat tttttcagaa agaaaaaaac
    15721 tcttacctta caatccttcg aattttactg tttttacaga attaaggcag aatttgaaat
    15781 cgtaaaacca ttttgaataa gagttgcttc aatccattga ctccgactaa ttatgttgct
    15841 ttcgtgaatg ccaaaatcag ccgcaatttg ttcataagtg cgctattctc gcatgtattg
    15901 aatagttacc ataaggagat cgtctaggct taatttgctt tttcgtccac cttttgcgcg
    15961 tttacgttga taagctgttt ttaacaccgc taacatctct tcaaaagtcg tgcgctgaac
    16021 acctacaagg atcttgaaac ggctatcgct caattgttta cttgcttcat cattcatagt
    16081 tttattgtat tatatttttg tttcgcagga agtctattat tcctttcgtt ccaaggtttt
    16141 tacgctttta gcaacaagct tacacactcc acgtgatgcg tctgtagaac tcaaaaggtt
    16201 cggggaacct tttgaggatt aattgcgttt atctaacaaa ctcacacatt caacatggca
    16261 tgactgatga agtcatcata agaatttcac ttgctgctct tttacggtgg cagcctgaac
    16321 ggtcagaagt atcattgtat atcatttgta tcatggcata taaagtatcg ctctatttta
    16381 ttgatggttt taatatcgct cttatcatgg cgaaccattc aaatctgctc cacaaatgag
    16441 gaaagtacca ttttggacta ttcaattgtc aaagaacaaa taaaaaaaat cttcgcattt
    16501 tcacactcta tgaaattgag gagattattt tctaaagcga attttatcag gaaaatccgt
    16561 caatcccaat aggtagtctg tactgacatc gtaaaagttt gagagagtta ccaatatatc
    16621 tgctgttaac gtatgctcac cccgttcaat tttagcataa gctgagtctg taaacgaaag
    16681 tattttagct atttgttttt gggtcaaatc gtgatcttcc ctcaaatctc tcaaacgtct
    16741 gtacatctag ctttctcctg agaaaagta
//
LOCUS       DAVBDG010000041        16725 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBDG010000041 DAVBDG010000000
VERSION     DAVBDG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.042"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..209
                     /locus_tag="VQ526_001410"
     CDS             <1..209
                     /locus_tag="VQ526_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HET2555123.1"
                     /translation="AGAKIIEKSFSYRDYQKYRQSSHKFELKQRLYFLLQQSKSFDDF
                     LEKAKQLHVQIDFSQKHSRFTSRR"
     gene            191..505
                     /locus_tag="VQ526_001411"
     CDS             191..505
                     /locus_tag="VQ526_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HET2555124.1"
                     /translation="MHVETVALLSKLDVDKHISVEIELDEMDLTSAESKATYAQIKEY
                     VWNKFELKVSTLYIAQIKKKCGIELREHYNKSKKDKQIIPQCTPEKEEAIMDALRHFK
                     MI"
     gene            719..910
                     /locus_tag="VQ526_001412"
     CDS             719..910
                     /locus_tag="VQ526_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HET2555125.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSW"
     gene            1205..1423
                     /locus_tag="VQ526_001413"
     CDS             1205..1423
                     /locus_tag="VQ526_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2555126.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            1816..2841
                     /locus_tag="VQ526_001414"
     CDS             1816..2841
                     /locus_tag="VQ526_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2555127.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            2831..3838
                     /locus_tag="VQ526_001415"
     CDS             2831..3838
                     /locus_tag="VQ526_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2555128.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            3838..4845
                     /locus_tag="VQ526_001416"
     CDS             3838..4845
                     /locus_tag="VQ526_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2555129.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            4845..5639
                     /locus_tag="VQ526_001417"
     CDS             4845..5639
                     /locus_tag="VQ526_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555130.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            6027..6416
                     /locus_tag="VQ526_001418"
     CDS             6027..6416
                     /locus_tag="VQ526_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2555131.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            6382..6696
                     /locus_tag="VQ526_001419"
     CDS             6382..6696
                     /locus_tag="VQ526_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555132.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            7380..7541
                     /locus_tag="VQ526_001420"
     CDS             7380..7541
                     /locus_tag="VQ526_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555133.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            7633..8481
                     /locus_tag="VQ526_001421"
     CDS             7633..8481
                     /locus_tag="VQ526_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2555134.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVW"
     gene            8527..9312
                     /locus_tag="VQ526_001422"
     CDS             8527..9312
                     /locus_tag="VQ526_001422"
                     /inference="COORDINATES: protein motif:HMM:NF024800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HET2555135.1"
                     /translation="MNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKYKDFY
                     YYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVDTS
                     EIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDKIDEL
                     ESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEIQVYE
                     EKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            9580..10146
                     /locus_tag="VQ526_001423"
     CDS             9580..10146
                     /locus_tag="VQ526_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555136.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            10469..10657
                     /locus_tag="VQ526_001424"
     CDS             10469..10657
                     /locus_tag="VQ526_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2555137.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            10719..11477
                     /locus_tag="VQ526_001425"
     CDS             10719..11477
                     /locus_tag="VQ526_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2555138.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            11535..12389
                     /locus_tag="VQ526_001426"
     CDS             11535..12389
                     /locus_tag="VQ526_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2555139.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            12501..13385
                     /locus_tag="VQ526_001427"
     CDS             12501..13385
                     /locus_tag="VQ526_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET2555140.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLINKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            13548..15285
                     /locus_tag="VQ526_001428"
                     /pseudo
     CDS             13548..15285
                     /locus_tag="VQ526_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            15560..16015
                     /locus_tag="VQ526_001429"
     CDS             15560..16015
                     /locus_tag="VQ526_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555141.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            16460..>16725
                     /locus_tag="VQ526_001430"
     CDS             16460..>16725
                     /locus_tag="VQ526_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2555142.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANL"
BASE COUNT         6113 a         2290 c         3235 g         5087 t
ORIGIN      
        1 ttgctggggc gaaaattatt gagaagagtt tttcttaccg tgactatcaa aaatataggc
       61 agtctagtca taagtttgaa ttgaaacaac gtctttattt tttgctgcag caatctaaat
      121 cgtttgatga ttttttagag aaagcaaagc agttgcatgt tcagattgat tttagtcaaa
      181 agcatagtcg attcacgtcg agacggtagc acttttgtcc aaacttgatg tcgataagca
      241 cataagtgtt gaaattgagc tggatgagat ggatttgaca agtgcggaga gcaaagcaac
      301 atatgctcaa atcaaagaat atgtttggaa taaatttgaa ttaaaagttt cgacattata
      361 tattgcacag ataaaaaaga aatgtggaat agaattacga gaacattaca acaagtctaa
      421 aaaggataaa caaattattc cacagtgtac acctgaaaaa gaagaagcca tcatggatgc
      481 tttgagacac ttcaaaatga tttaatagaa aagaatgaca gtatatgact ttctgcattt
      541 attacattcc tacttggtat aggaacagct attattcctt tcttgcaagg tatcaattag
      601 aaaataggct caatataaag attgatagga tcatttttat atttaaagga gcgttgaaat
      661 gattgataaa ggcaacaaaa aattttagga taaatttgct aagttgtatg ccccttttat
      721 gaaaaaagat aaagaggttt atgataaagt ttgtgaatat cttagtcctc atttgaataa
      781 agatatggag gtgcttgaac ttgcttgttg gtttcgtgtc ataacagtta tagaggcaaa
      841 tagttatgta aatataagga gttcaagact tctaccaaag tttaaaactc aaaaaataaa
      901 tagttggtaa gcagcatacc aatgaattta aagattcttt aaagagtctt attttgtgat
      961 gaaaatttaa tatgtaaatc tcagacgata gaaattaaaa actctatcgt cttttttata
     1021 ctcaaaatta ggaggtaaaa atggtaagga taagaggtcc cacttaaaac aatttatggc
     1081 aaaataagga cggaataaca caacaaattc tctaaaacaa atcactaaat caatgtaaga
     1141 ttgaataaaa tcaatattta tgctataatt aaataaatct aatgaagaaa aaaagaggga
     1201 tattatggca cttaactata aaccattatg gatacagtta gcaaaaaaag gactaaagaa
     1261 aacagatgta atagctatgg caggacttac aacaaatgtt atggcacaaa tgggaaagga
     1321 taaaccaatt acatttaaga atttagaaag aatatgtaag gctttatctt gcactcctaa
     1381 tgatattatt agttttgaag ataattttag tgacgaggaa tagaaaatga ctttaaggac
     1441 agaagatcaa gttagggatt atgcaagaga agtataggct ttaatgaagt tgaagaaaac
     1501 atcaatcaag gtactggtca aataactact tttaatcaat taggcttcaa gggatattca
     1561 gataagccag atggttggta tttacctaaa aatatgaatg atgtagcaat aatccttgaa
     1621 acaaaatcag aagaaagaga tattagcaaa caaattttta ttgatgagtt aatgaaaaat
     1681 atagacataa tttaactaaa aataaaaact agatcctttt ttgaaaaaat tatattatta
     1741 aatttgtaac tgtatctatt gacaatgata attattatcg atacaataga cttgaaatat
     1801 gtttaaggag tttttatgaa aaacaaattt tttctaatag ctattttagc tatgtgtata
     1861 gtttttagcg cttgttcttc taattctgtt aaaaatgaag aaaatacttc taaagagcat
     1921 gcgcctgata aaatagtttt agatcatgct ttcggtcaaa ctatattaga taaaaaacct
     1981 gaaagagttg caactattgc ttggggaaat catgatgtag cattagcttt aggaatagtt
     2041 cctgttggat tttcaaaagc aaattacggt gtaagtgctg ataaaggagt tttaccatgg
     2101 acagaagaaa aaatcaaaga actaaatggt aaagctaacc tatttgacga tttggatgga
     2161 cttaactttg aagcaatatc aaattctaaa ccagatgtta tcttagcagg ttattctggt
     2221 ataactaaag aagattatga cactctatca aaaattgctc ctgtagcagc atacaaatct
     2281 aaaccttggc aaactttatg gagagatatg attaaaattg attcaaaagc cttaggtatg
     2341 gaaaaagaag gtgatgagtt aatcaaaaat actgaagctc gtatatccaa agaattagaa
     2401 aaacatccag aaatcaaagg aaaaatcaaa ggaaaaaaag tattatttac tatgattaat
     2461 gctgcagata catcaaaatt ctggatttat actagcaaag atccaagagc aaattattta
     2521 acagatttag gtctagtttt ccctgaatca ttaaaagaat ttgagagtga agatagtttt
     2581 gcaaaggaaa tttctgcaga agaagcaaat aagataaatg atgctgatgt aatcataact
     2641 tatggtgatg ataaaactct tgaagcttta caaaaagatc ctcttttagg taaaataaat
     2701 gcaattaaaa atggtgccgt tgctgtaatt ccagataata caccgttagc agcctcatgc
     2761 actccaacac cactttcaat aaactatact attgaagaat acctaaatct tttaggaaat
     2821 gcatgcaaaa atgcgaaata aaaaacaaat aaacctaggc ataatttttg taatctgcct
     2881 aggtcttctt attacaatat ttttgtcatt aaagcttgga acaaaagaaa ttaatatcag
     2941 agatttttta gcagcttttg gaatgggtaa tacaaatgat gattttatta aatcaattat
     3001 atataaaaga atacctagaa ctatttttgc aattttagca ggttctagtc ttgccataag
     3061 cggtgtattg atgcaatcag ttactagaaa cccaatagct gatccaggta tactcggtat
     3121 aaacacagga gcaagtctta gtgtagtaat tggtctttct tttttaggaa tttcatcaag
     3181 cataagccat ataagttttg caatcattgg tggcttagta agtgcaattt ttgtatacgc
     3241 gattgctgta agcggaaaag caggccttac ccctataaaa cttgccttat caggaacttg
     3301 tgttagtatg gctttaagca gttttgtaag ttttttaatt ttaccgaata ataacgtctt
     3361 agacaaattt agattttggc aaataggtag ccttggagca gctacattat cttctatatc
     3421 tacactacta ccttttataa ttttaggtca cttgatagct atatttattt catcagattt
     3481 aaacgcttta gctatgggtg atgaaatggc tgttggtctt ggagttaatg ttaataggat
     3541 aagatcactt gcaataattg caagtgtgct tttatgttca agtattactg caattggtgg
     3601 acctattggc ttcgtaggtc ttatagttcc tcacttttgt ggcttattta taagcaaaga
     3661 tatacgcaca atgaccattt cttcatcttt tataggtgca gagctcttgc ttatatgtga
     3721 tataatcggc cgtatgttag gtaaaccagg tgaaattgaa gtagggataa ttactgcaat
     3781 aatcgggggt ccagtactta tttatgtaac tatgaaaaat agaggggtta ataactaatg
     3841 caaaatttaa ttataggtat tcaaaaaaga aaaaatagaa taacactatt ttcctcacta
     3901 tttttattaa taataatcag tctatcattt ttcattttac ttatcggaga tgaaagttat
     3961 tctttttcaa ctttgattaa agtcttaaat agtgaaactg ttcctggagc tagtttttcg
     4021 attatggaaa ttagattacc aaaattatta gcaggaatta tagctggctg gtcttttgga
     4081 ttggcaggat ttatctttca aactatgtta agaaatcctc ttgcaagtcc tgatataatc
     4141 ggtgtcacaa gttcttcatc tattgcagcg gtcttttgca tattggtatt aaaaacaaat
     4201 agtttaacta ctggaattat ttcaataact tgtggactaa catcatcttt aatattattt
     4261 ttactagcta aaaaagatgg tttttcagca gcaagactga taatattagg cattggtttt
     4321 caagctgtca caagagcagg cacctcattt ttattgttga aagtagcaag atatgaatta
     4381 caagaagtta tgagatggct cagtggctct ttatctttta caaagttaga tgacatacct
     4441 cttgttctaa tagtaagtat tattgctact atattagttt tattttttaa taaaagacta
     4501 gaaattattg aacttggtga agaaatagca atcggacttg gagcaaatcc cgagctttca
     4561 aggcttgttt taattttttg cgctgtatct ttaactgctt tttctacttc aattacagga
     4621 ccaatagctt gtatatcttt tttagctggt cccatagcct taaatattgg caagaaaaga
     4681 agcccaatat tagctggatt ggttggaatt ttactagttt tgttatcaga catattctct
     4741 caaaatattt taccagctag atatccagta ggtgttgtaa ctggcttgtt aggttcacca
     4801 tacttaatat acttactaat aaaaatgaac aggaggaata tataatgaaa ggtctgtggt
     4861 caaataatct tacctgcggt tatgatgaaa aaataatctt ggaaaatata aatataaaaa
     4921 tacctgaaga aaaaatatca gttattattg ggtcaaatgg ttgtgggaaa tcaacactca
     4981 ttaaaacctt gtctcgactt ataaagccat tagagggaga agtattgctc gataataaat
     5041 caattaattc ttataaagaa aaagatttag caaaacacat agctatatta cctcaatctc
     5101 caataatccc tgaatcaata acagtagctg atcttgtaag ccgtggtcgt ttcccctaca
     5161 gaaagccttt taagagtctt ggaaaagatg accttgaaat aataaacaga tcaatggtta
     5221 aggccaatgt tgaagatcta gcaaataacc tagttgaaga actttctggg ggtcaaaggc
     5281 aaagagtatg gatagctcta gccctagccc aagatacaag tatcctactt ttagatgagc
     5341 caactactta cttggatatc tcatatcaaa tagaactatt agacctcttg actgatctaa
     5401 accaaaaata taagacaacc atttgcatga ttttgcacga tataaatcta acagcaagat
     5461 acgctgatta cctatttgca attaaagaag gtaaacttgt tgcagaggga aagcctgaag
     5521 atatactaaa tgataaacta gttaaagata tctttaatct tgaagcaaaa attatacgtg
     5581 accctatttc caattcgcct ctaatgattc ctattggcaa gcaccatgtt agctcttaat
     5641 aattgaatat cggaattcat ttaaaagtcg caaattttga actgaccctc aaaagttagg
     5701 ccaaaaaact aactaaagga ggtcagtttt ttaatggcaa atacagcaca gaatttaaga
     5761 taaaattagt aaaagaatat catgaatgaa aaataggata tagagaataa cagcaatatt
     5821 aaaaagatca ggactgataa taaacaaaaa gaaagtacaa cgactagttc aaaagctaaa
     5881 acttcaagta aaaagttatt caagaaaatc tagaaaatac tcatcctaca aaggacaggt
     5941 aggaaaaata tcggacaaca aaataaaaag aaatatttat taccgaaaaa aaggggaaat
     6001 cgagtcaagt accaacaggc tcatctattc caaaaagata tttactcgat ggaaaaactc
     6061 ctagaattag tgctacaaat attgacaatg gtatcttagg ttactacgaa gatatagatg
     6121 ataaaaatta tagagttttt gaaaatttta tatctgtttc atttttagga gcagtctttt
     6181 atcataaata taaagcaagc ctagatatga aaattcattg tttaaaatta aaaaataaag
     6241 aattgaataa agaagtagca ttctatttaa cttcaattat taggcaagct ttaaaaaata
     6301 ctgaatataa agatcaaata tctagcacag tattgccaga tatcaaaatt aaattaccga
     6361 tagacagtag aggtactcca gattggaatt acatggaaag atatagagat agataaagag
     6421 agagagggag agatagataa aaagtccacc cccatttttt atggagaatt caaaaatgta
     6481 aggttaagca aagaagaata taaaaatcta aaagaaaaat taaactcaca cacagatata
     6541 atgataaata aactatccag atacatggaa agcagtggta agacctatca aaaccactat
     6601 gtgacaatct taaaatggta tgaagaagat aaagacaaac taagacagaa aggtttaaat
     6661 aaaaaaatga attatgatgt aggagaatct ttatagataa aaagaggtgg aaaggcacta
     6721 ttaaagactt taagtctaaa aaggtatcaa agtatgccag agataaaaaa atataaatat
     6781 gacactttaa aggcgattag aaaattaaag aaatgctaga agacttttag atgacagtta
     6841 ccaagagggt ctaattttca ctgctactta tgcagtggga atttgacctt tttatataag
     6901 caaattctat ttcaaaattg gttaccaaga aagacttctt ttcactgtta agtataagag
     6961 ggtaatttat agaattttgt tacattaaga taaatatttt ctgatggtca aattttaaaa
     7021 agattaataa ctctcgataa attttgaacc ttaacaattg aatagaccaa gacaagacgt
     7081 taattatttt tggagcgtaa taacctatcc gtcaagatct ttcggctgaa taattcggca
     7141 aaaaaagttc tgctaagtaa aaagaaagtg agcgataagt aagagtttga atatagggag
     7201 ggatacccta tccgctatga agaaaatgag gataatgata cttctaaggt tgaagccggc
     7261 tcatccggaa tagaggcgga ggtgagattc ctatgttgct aatccaaagc acttgtcaat
     7321 gtcaaaaaac ttaattaaac aaataattat tagatataaa actttagagg aggtatatat
     7381 tggacaatga ctggaatgga ttggctgatt taatagcaaa tctgattgca aaatatgctg
     7441 gtgctttaga tttagataat ctacccgatc caacgccagc aaaaaatcaa gagatgaaaa
     7501 atagttttga catggcaaaa acacaaattg agacggacta ataaaagtgt tataatatac
     7561 ttgaaatgat agtccaaact taaattaaaa aaattatata tgatatagaa aaattatatt
     7621 gaggttaata ctatgtcaaa agaaaaaata aaagtatacc tctatacacg agtatctacg
     7681 tcaatacaga tagaggggta ttcgttagag gcacaaaaat cacgaatgaa agcttttgct
     7741 atttacaacg attatgaaat tgttggagag tatgaagatg ccgggaagtc tggcaagtct
     7801 attgaaggta gaatacagtt caatcgaatg atggaagata taaaatctgg aaaagatgga
     7861 gtatcttttg ttcttgtgtt taagttatca agatttgcaa gaaatgccgc tgatgttctt
     7921 tctacacttc agataatgca ggattatgga gttaatctta tctgcgttga agatggcatt
     7981 gattcatcta aagatgctgg gaaactaatg atttctgttt tatcagctgt ggctgaaata
     8041 gaaagagaaa acatccgtat tcaaacaatg gaaggttgca ttcaaaaagc aagggaagga
     8101 aaatggaatg gtggctttgc tccgtatgga tataaacttg aagacggcaa gctgtttata
     8161 aatgaggaag aggcagttgc aataagaacg attttcgacc agtatgtaaa tactacgata
     8221 ggagccaatg ggatatctaa atacttagag aatcatggaa ttagaaaaat cccaagacag
     8281 aatggtaaga atcctttgtt tgatgcaggt cttataagaa agatattaaa gaatcctgta
     8341 tataatggga aaatagcatt tggaagaaga actttagaaa aagttcatgg tacaagaaat
     8401 gaatataagc aggttgaaca agatgaatat ctaatatctg aagggataca tgaagctata
     8461 gtttccgatg aagtttggta agctgctcag gttaagctaa aatctcaagc aaagaaatat
     8521 gagcatgtga ataaaggaaa agatacacgc acacacttgc tttctggaat tgtaaaatgc
     8581 ccgatatgtg gagtgggaat gtttgggaac aagtgtatca agaaaaagaa agatggcaca
     8641 aagtataaag atttttatta ctatggttgt aaccataggc agatgataag aggtcataag
     8701 tgtacattca gtaagcaaat tagagaagaa ttgttagatg atgctgttgc agaggtgatt
     8761 gtcaagatag taagtaatcc gaaatttgct tctatgatgc aagaaaaaat caacatgaag
     8821 gtggatacct ctgaaataga aaaagaaata gataattacc aaaaggaatt gagaaagagt
     8881 cattctacaa agtttaagct aattgaggaa atagataatt tagatgttga agataagcat
     8941 tataagagaa ggaaacagga tttagatgat agactatatc gtatgtatga caaaatagat
     9001 gaattagaat catcactaat tgatgcgaaa gcaaagaaac agactattga agctgaaaag
     9061 cttacaggag ataacatata taaggttctg atctattttg ataagctcta taaagtcatg
     9121 aatgatgtag agcgtaggca gttaatttca gctttgattt ctgaaattca agtttatgaa
     9181 gaaaaacaat ctaacggaca atggctaaaa tccattactt ttaagctacc aattatagaa
     9241 gaaaatctca acatagattt ggacaatgat gaacaagttg aatgtgtaag tttattagag
     9301 aaacgtagtt aatcctcaaa aggttcccca aactttttga gtcccactga cgcatcacgc
     9361 tgaggcggta tcactgcttg tacgagttga agcatcagcg aagtaaaagt agatgttcag
     9421 ccaatgcgac gaggtgagac cgaaggtcga taaggtgtgt tctgggtata ggactcgtaa
     9481 agtaagtagg aaatatgatg agatgataca gagagcctga attggtcagg tgacgtgctt
     9541 atttacatta gcctgataat gaaaatggag agattatcga tggttatatt ggaattgtac
     9601 caaaatgatt attctaaaga tttagtagca tttgattcca tagaagatgg gaaagccttt
     9661 gtagctcaaa tcccgggata cactctggaa acagaagatg gttttgaagt ggagtatttt
     9721 aatcctaaaa acataccgga ttatatggaa attattttta acggaaatat tgttccttta
     9781 tctaaattta tgttcgatcc tgaggaaaat gtaaacatta tttggaaaga gatttcaaat
     9841 ttatccctca aaaatgatag agtgatcgaa ggatattcaa aaattgatgc atatgtcgtt
     9901 aacaaccatg aagttaaagc ctatgtcgaa acaagagaaa cgaactatcg taaagcaaaa
     9961 gacttcttag aaagccgcgg atatgaaatc gacagaagct ttttcggaag tgaagacggt
    10021 gaagcgattc tttacagaaa aaaagggatc gaagtttggc atttcttgtg tcacttagac
    10081 ccaatgtttg tagagattga agatgtagaa ggatatgtta aagaagaagt tggagagatt
    10141 caatagtcaa ccaaagttcc ttgtgagtat atacctttgc tagtaaaaga gtcaaaacgt
    10201 tataacgaag gggggggaag aaaacccttg agtgtaagtc tttttcttgt ttagagggaa
    10261 agtattagag tagccgctca taatttatac tagatttgat aactgatttg agacagattt
    10321 tatttctata aatattgtga aaatttttta ataccaaaga tttaaagtta ctgtgttaaa
    10381 aatcctctcc acttttaaac taatgtatta taaaagccag ttgacacaag tgttacacct
    10441 gtgatagaat taactcgagg tgatgaaaat ggcaaccgat aaaaatagaa taatgattag
    10501 cttggatgat aaaaatctag agaaacttga aaatttagta gaagatgcta gagatagaag
    10561 gggaatgcga ctaacaaagt cccaggttat tgaattactc ctaaataccg ttgattattt
    10621 cgatgatatt atgggggcta tttactcaaa aaaataagag caaaattgct attatttttt
    10681 acaaattggt gtaacactag tgatatagga gtgatgaaat ggttgtttat attagacaaa
    10741 gtaaattacc aagtgaggtt tcaattaata aatacaatgc tcaagtaggt gcctacttac
    10801 aaggagaaga agttatttta tatcaatctt tttctgaaat aaaagagtta acaagtgaag
    10861 atatagttgt agattatata atggagacta gagcattact aaaaatgatg ggcttaaacg
    10921 ttccggttca tgactatcct attgagctta aagagtttta tggtcgaaag atttatgctg
    10981 gtattttagg agagattgtg aatatacctg ataattgggg aaaatttatt aagcctaaag
    11041 ctggttcaaa agtctttact ggaagagttg ttaatggaac ccatgattta ataggtattg
    11101 gtctaccttt cgactatcct atatggatta gtgaggttgt agaattcata gctgaatggc
    11161 gttgttttgt gttagatggt cgcgtattag atgttcgacc ctatacaggt gattatcatg
    11221 cacaatttga tgcaagtgta attgatgaag cgatatcatg ttggaaagat gcgccaatag
    11281 cttatggact agatatcggt gttactcgcg atggcagaac acttgttgtt gaagtaaatg
    11341 atggttatgc attgggaaat tatggcctat ctcctttaaa atcaatcaat tttcatagag
    11401 ctaggtggaa agaaatggta aaaccctatt ttgaaaaaaa tgaaattttt aaaatccaac
    11461 aggatgttat tttctaaaat aaatatggta gataaccaat ataaaaagca tcaaaatgaa
    11521 caaggaggaa tctgatgctt ggagcaatta ttggagatat tgtcggttct gtttacgaat
    11581 ggaacaatat caaaacaaag gactttcctt tatttcggaa ggactgcttt tttacagatg
    11641 acacggttat gacctgtgct gttgcagaag caattatgaa tggtggacaa aaagacgatt
    11701 tcattgacgc gatgaagaaa tatggcagaa tgtatccgaa tgctgattac ggtgctcggt
    11761 ttaatgcatg gctaaacagc gataaccgtg agccttataa tagctttggg aatggatcag
    11821 ctatgcgtat ttctccatgt gcttggatca tggactgtgg tttttatgcg aaaactggta
    11881 tgtggccatc atctagagga cttacgagtc tttctgcaga ggtaactcat aatcatccag
    11941 aaggtgtcaa aggcgctatg gctacagctg atgctatctt tctatgtcgt ttttactttg
    12001 gtggttattg tagagagtac gagcaatcaa ttaacgacaa tcctacagag tgtaaaagac
    12061 gaattaagga ttatatagaa aaagaatacg attacaatct atctcaaact ttagatgaaa
    12121 tccgtcctaa ctatcgtttt aacgaaacat gtcaggaaac tgtccctcaa gccattatcg
    12181 cctttcttga aagtagagac ttcgaagatg cgataagaaa tgccatctca cttggtggcg
    12241 acagtgatac tctcgcagca atcacttgta gcatagcaga ggcagcttac ggtattcctg
    12301 attggatcaa ggataaggcc tattcttact tggatgaacc cttaaaggat gtagttaggc
    12361 gatgggaaaa tagaataaaa gcgtattaat ctccagtatg tcagatttct cttttctgta
    12421 ttttcattgt ataataggat tacatttact tgcatacgac gaaatgtaaa catttctgtt
    12481 taaaattaag gataaaatca atgaaaaaag caatggtaat tatcaaccct acttctggtg
    12541 gcgagaaggc tttggattac aaagaaaagc tggagaataa agcaaaagaa tactttgaat
    12601 atgttgaaac caaaattacc gaaaaagcgc tggatgcaac acattttgct gaagaagctt
    12661 ctcgtgagca gtatgatgca gtggttgtgt ttggtggaga tggaactgtc aatgaagtca
    12721 tttcaggtat tgatgagaga gactacattc ctaagttagg gattatccca ggcggtacgg
    12781 gtaacctcat tacaaaactt ttggaaatca atcaagacat cgatggcgca attgacgaac
    12841 tggattttga tttaatcaat aagattgata tcggtaaagc aaatgacaac tattttggtt
    12901 atatctttag tatcggttct ctgcctgagg cgattcacaa tgttgaaata gaggacaaaa
    12961 caaaattcgg tattttaacc tatgctgtaa ataccatgaa gtctgtcatg acagatcagg
    13021 tctttaacat taaggttgag acagaaaatg gaaattatgt tggtgaagct agccatgttt
    13081 tggtcctttt gacaaattac ttcgctgata agaaaatctt tgaagaaaac aaggacggct
    13141 atgccaacat tttgattctg aaagatgcct ctatattctc caaattatcc gtcattcctg
    13201 atttattaaa aggggatgtt gtcgcaaatg ataatatcga gtatatcaaa gcgcgtaata
    13261 ttaaaatctc ttcagatagt gaattggagt cagatgttga cggagataaa tcagataacc
    13321 tacctgtaga aatcaaagtc ctagctcagc gagtagaagt attttcaaaa ccgaaagagg
    13381 attagtatat agagaaagcc ttttttaagg ctttttgtat actttaaaag atagttcctt
    13441 taacaacgga cattccttgc aaatagttta caaaaatagt atactggatt cattgagttt
    13501 gaaaacgttt gcgtaaaatt tgaatgaata ctttaggaga caaattgatg gaattgagtg
    13561 ctatttacca taggcctgag tcggagtatg cctatcttta taaggataag aaactccata
    13621 ttcgaattcg aactaagaaa ggggacattg aaagcatcaa cttgcactat ggggaccctt
    13681 ttatctttat ggaggagttt tatcaggata caaaagaaat ggtcaagata acttctggta
    13741 ccttatttga ccattggcag gttgaagtgt cagttgactt tgcacgtatc cagtatctct
    13801 ttgagctcag agatacagaa ggtcaaaata ttttgtatgg cgataaaggg tgtgtggaaa
    13861 attctctaga aaatcttcat gcaattggga atggatttaa gttgccttat cttcaagatt
    13921 gatgcctgca aggttcctga ctgggtttca aatacggtat ggtatcagat atttcctgaa
    13981 agatttgcca atggcaatgc tctattaaac ccagaaggga ctttagactg ggattcatct
    14041 gtcacaccta agagcgatga tttctttggt ggtgatttac aggggattat tgatcatatg
    14101 gattacttgc aagacttggg tattactgga ctatatcttt gtcccatctt tgaatctaca
    14161 agcaatcaca agtacaatac gacagattac tttgaaattg accgtcattt tggagacaag
    14221 gagacctttc gggaactggt ggatcaagcg catcatcgtg gcatgaaagt catgctggat
    14281 gcggtattta atcatattgg ttcgcaatct cttcaatgga aaaatgtcgt caaaaatggt
    14341 gaacagtctg cttataagga ttggttccat attcaacaat tcccagtgac aactgaaaaa
    14401 ctagttaata agagagactt accctatcat gtttttggtt tcgaggacta tatgcctaag
    14461 ctaaatacag ccaatccaga ggtcaagaat tatcttttaa aggttgcgac ttattggatt
    14521 gaagagttta atatcgatgc ttggcgtttg gatgtggcta atgagattga ccatcagttc
    14581 tggaaggatt ttcgtaaggc agttttagct aaaaatcctg atctttatat cctaggagaa
    14641 gtctggcata catctcagcc ttggctaaat ggagatgagt tccatgccat catgaattat
    14701 cctttatctg atagtatcaa ggactatttc ttacgaggaa ttaagaagac agaccagttc
    14761 atcgatgaaa tcaatggaga gtctatgtat tacaagcagc agatttcaga ggtcatgttt
    14821 aatctcttgg attcacatga tacagagcga atcctgtgga cggccaatga agatgttcaa
    14881 ctggttaaat cagccttagc ctttctcttt ttacaaaaag gaacaccgtg catttattac
    14941 ggaaccgagc tagccttgac tggaggacca gatccagatt gtcgtcgttg tatgccttgg
    15001 gaacgtgtat caagtgacaa tgatatgctg aactttatga agaggctgat taagattcgg
    15061 aaatacgcgt cagtaattat ttcgcatggc aagtatagcc ttcaagaaat caaatctgat
    15121 ttagtagctc tggaatggaa atacgaagga cggatcctca aagcaatatt caaccaatca
    15181 acagaagatt atcttttaga gaaagaagca gtagcactag caagcaattg ccaagaattg
    15241 gagaatcagc ttgtcatctc tccagatgga tttgtgattt tctaaaaact agttgatgaa
    15301 gattatggta catttcatat cttatatagt ataataaggc tagttactaa acttgtaaag
    15361 gagaacttaa atgaattgta gaggacatga aacaagacaa agaattgtta gagattttga
    15421 agtttagcct aaagcacata ttaagctgtt agcaaatcaa caaaaacata gtgatgcagg
    15481 agcaactatt gaagatgaat attatgtatt tatcgctgag agtaaaattg atggcaagaa
    15541 ggaagttatt cagtgttgca tgggtgcggc aagggatttt ttagaactaa ttaatcacaa
    15601 agggctacct ctttttaatc cacttgtagg tgattctcat gtaaataata gacaagaata
    15661 tgacaataca gggagtggaa atttacaacc tgaaaagtgg aatgaaactg caaagcagct
    15721 ttataatgct ataatgtggt tgattatttt atggaatgct aagccggata cacctttatt
    15781 taattttaaa gacgaagtaa ttaagtataa aacatatgag ccttttgaaa gcagtataaa
    15841 aagagtaaat actactataa agaatggtag taaagggaaa actctgactg agatgattaa
    15901 tggctacaga gcggataacg atattagaga tgaaatttgt aactttaata ttctgaaaaa
    15961 taaaattcgt gatatgaaag accaacaagg aaatacaatg gaatcttact tttagttatt
    16021 gttgaatttt gggtattcta taaaatatcc taattgagat ttaaatagta gactatacaa
    16081 tatagttaaa atatcagtaa aaacaacact ttattgaggt attggatacg ctttgctaat
    16141 agcctaataa tcacatgtgg agtgttgcta caacgaaaaa ggtgataatc cttgatttca
    16201 agctatttta taagcatttt gtctttgtag ataaaggcaa ttttgacaat aaaaatccta
    16261 aaaggtgaat cgttatagat gtatttgtag atatcgtttg cgcatcgaaa aaattaatac
    16321 aagaataaat atttatggct ctttaggtga cttttataga agtaaagttt aggatagaaa
    16381 aacaagaaat aacgcaccaa aattggtgta ttatgctttt ttatgctata atggatttat
    16441 aaaaataaag gagtttgcta tgattggaaa gaacataaaa tccttacgta aaacacatga
    16501 cttaacacaa cccgaatttg cacgaattat aggaatttct cgaaatagct tgagtcgtta
    16561 tgaaaatgga acgagttcag tctctacgga attaattgac atcatttgtc agaagtttaa
    16621 tgtatcttat gtcgatattg taggagaaga taaaatgctc aatcctgttg aagattatga
    16681 attgactttg aaaattgaaa ttgtgaaaga aagaggtgct aatct
//
LOCUS       DAVBDG010000042        16705 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBDG010000042 DAVBDG010000000
VERSION     DAVBDG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.043"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            32..496
                     /locus_tag="VQ526_001431"
     CDS             32..496
                     /locus_tag="VQ526_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2555143.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            654..1079
                     /gene="lacA"
                     /locus_tag="VQ526_001432"
     CDS             654..1079
                     /gene="lacA"
                     /locus_tag="VQ526_001432"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2555144.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            1110..1625
                     /gene="lacB"
                     /locus_tag="VQ526_001433"
     CDS             1110..1625
                     /gene="lacB"
                     /locus_tag="VQ526_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2555145.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1636..2565
                     /locus_tag="VQ526_001434"
     CDS             1636..2565
                     /locus_tag="VQ526_001434"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2555146.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYCASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFTKHGDTFYKVDIPRIQAVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2567..3547
                     /gene="lacD"
                     /locus_tag="VQ526_001435"
     CDS             2567..3547
                     /gene="lacD"
                     /locus_tag="VQ526_001435"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2555147.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3781..4617
                     /locus_tag="VQ526_001436"
     CDS             3781..4617
                     /locus_tag="VQ526_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2555148.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4653..4970
                     /locus_tag="VQ526_001437"
     CDS             4653..4970
                     /locus_tag="VQ526_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2555149.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            4970..6661
                     /locus_tag="VQ526_001438"
     CDS             4970..6661
                     /locus_tag="VQ526_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2555150.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            6876..8282
                     /gene="lacG"
                     /locus_tag="VQ526_001439"
     CDS             6876..8282
                     /gene="lacG"
                     /locus_tag="VQ526_001439"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2555151.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNVKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTTLEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIIRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8372..8584
                     /locus_tag="VQ526_001440"
     CDS             8372..8584
                     /locus_tag="VQ526_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2555152.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(8863..9624)
                     /locus_tag="VQ526_001441"
     CDS             complement(8863..9624)
                     /locus_tag="VQ526_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2555153.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9806..10768)
                     /gene="nrdF"
                     /locus_tag="VQ526_001442"
     CDS             complement(9806..10768)
                     /gene="nrdF"
                     /locus_tag="VQ526_001442"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2555154.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10956..13115)
                     /gene="nrdE"
                     /locus_tag="VQ526_001443"
     CDS             complement(10956..13115)
                     /gene="nrdE"
                     /locus_tag="VQ526_001443"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2555155.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13196..13414)
                     /gene="nrdH"
                     /locus_tag="VQ526_001444"
     CDS             complement(13196..13414)
                     /gene="nrdH"
                     /locus_tag="VQ526_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2555156.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13765..14028
                     /locus_tag="VQ526_001445"
     CDS             13765..14028
                     /locus_tag="VQ526_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2555157.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            14034..15767
                     /gene="ptsP"
                     /locus_tag="VQ526_001446"
     CDS             14034..15767
                     /gene="ptsP"
                     /locus_tag="VQ526_001446"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2555158.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            16022..>16705
                     /gene="phtA"
                     /locus_tag="VQ526_001447"
     CDS             16022..>16705
                     /gene="phtA"
                     /locus_tag="VQ526_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2555159.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAA"
BASE COUNT         5201 a         3014 c         3466 g         5024 t
ORIGIN      
        1 taaagaaact agcaaggtca ggccttactt tattggtgga catccaggat ttaattgtct
       61 tctacttgat gatgaagtct atgaagatta ttatctagag tttgagaaag aagagatttg
      121 ctctgttcca cgtcctttcc cagaaacagg tatgttggat ttccaagata gaagtccatg
      181 gctagaggga caaaaagaaa tagatcttag ttatgatctt ttcagcacag atgcagtgac
      241 tttggatgaa ttgcaatctc gaacaattgc ccttcgttct cttaaacatg ataagggatt
      301 gaaagtgcat tttgcagaat ttccaaacct catcatctgg tcaactttga acaagggacc
      361 tttcattacc tttgaaccat ggtctggctt gtcaacattc cttgaagaag gagatcattt
      421 agaagataag aagaatgttt gtctcttaga agccaatcag gttgaagaat tagggtttga
      481 gatagaagtt ttataaaaaa agtaaaaaat aaataacaaa aacaaacaca aactgttgac
      541 ttcttcaaac aatagtagta tattatgttt gtgaggacta attgactgtt ttttaaagac
      601 agccaataca ctgctggaat ccagcataga aaaaataaaa aggagtatac aatatgtcta
      661 ttgttatcgg tgcagatgct gcaggtttga gattgaaaga agttgtgaaa gacttcttag
      721 aaaaagaaaa cttccacctt gtggatgtta cagctgaagg tcaagacttt gttgatgtga
      781 ctctcgctgt tgctgcagaa gtaaacaaag aagaacaaaa ccttggtatc gtgattgatg
      841 cttatggagc tggtccattt atggttgcaa ctaagatcaa aggaatggtt gctgcagaag
      901 tatctgacga acgttcagct tatatgactc gtggccacaa caactcacgt atgatcacta
      961 tgggggcaca acttgttggt gatgaattgg ctaaaaacat cgctaaagga tttgttaatg
     1021 gtaaatacga cggtggtcgt caccaaatcc gggttgacat gttgaacaaa atgtgctaat
     1081 tagattatag taagaaaggt aagttaaaaa tgagaattgc aattggatgt gaccacatcg
     1141 taacagatga aaaaatggcg gtttcagaat ttttgaaatc aaaaggatat gaagtcattg
     1201 actttggtac ctatgaccat acacgtactc actacccaat ctttggtaaa aaagtagggg
     1261 aagcagtaac tagtggtcaa gctgatctcg gggtatgtat ctgtggtact ggtgttggta
     1321 tcaacaacgc tgtaaataaa gttccaggtg ttcgttctgc cttggttcgt gatatgacaa
     1381 cagcccttta tgctaaagaa caattgaacg ccaacgttat cggttttggt ggtaagatta
     1441 ctggtgaatt gcttatgtgt gatatcatcg aagctttcat tcatgctgaa tacaaaccat
     1501 ctgaagaaaa caaaaaattg attgcgaaaa ttgaacacct tgaaagtcac aatgctcaac
     1561 aaacagacgc aaacttcttc actgaattcc ttgaaaaatg ggatcgtgga gaataccacg
     1621 actaagaggt aacctatgat tttaacagtt acaatgaacc catccattga tatttcctat
     1681 cccttggatg agttgaagat tgatactgtc aatcgtgtgg tggatgtaac caaaacggct
     1741 ggtggtaagg gactcaatgt tacccgagta ctttcagaat ttggcgattc tgttcttgct
     1801 actggtttag tgggtggcaa acttggtgag tttttggttg aacatatcga taatcaagta
     1861 aagaaagatt tcttctcaat tcagggagaa actcgtaact gtatcgctat tctccacgga
     1921 gacaaccaaa cagaagttct tgaaaaaggt cctgaagtat tggaacagga aggtcaagac
     1981 tttttggaac atttcaaaaa actcttggag tcagttgaag tagtggccat ctcaggtagt
     2041 ctgccagctg gccttccagt tgattactgt gcgagcttgg tagaacttgc taatcaagct
     2101 ggcaagcctg tagtcttgga ctgctcaggt gcagcacttc aggctgttct tgaatcaccc
     2161 cataaaccaa cagtcatcaa accaaataat gaagaattgt ctcagcttct tggaagagaa
     2221 gtttctgagg atttggatga attaaaagaa gtacttcaag aacctttgtt tgcagggatt
     2281 gaatggatta tcgtttcact tggtgccaac ggtactttta ccaaacatgg tgacactttc
     2341 tacaaggtag atattcctag aattcaggcg gtaaatcctg ttggatctgg agactctact
     2401 gtggcaggaa tttcttcagg acttcttcac aaagaatcgg atgcagaatt actcatcaag
     2461 gcaaatgtcc ttggtatgct caatgctcaa gaaaaaatga ctggtcatgt caacatggcc
     2521 aactatcaag ctctatatga tcaattaata gtaaaagagg tataaaatgg ctttaacaga
     2581 acaaaaacgt gtacgcttag aaaaactttc tgatgaaaat ggtatcatct cagctcttgc
     2641 atttgaccaa cgtggtgctt tgaaacgcct catggctcaa caccaaacag aagaaccaac
     2701 tgtggcccaa atggaagaac ttaaagtttt ggtagcagat gaattgacta aatacgcttc
     2761 atctatgctt cttgaccctg agtatggact tccagcaact aaagctcttg atgaaaaagc
     2821 tggtcttctc cttgcttatg aaaaaacagg ttatgacaca acaagcacaa aacgcttgcc
     2881 agactgcttg gatgtttggt ctgcaaaacg tattaaagaa gaaggtgcag atgcagttaa
     2941 attcttgctt tactatgatg tagatagctc agacgaactc aatcaagaaa aacaagccta
     3001 catcgaacgc atcggttctg agtgtgtggc tgaagatatc ccattcttcc ttgaaatcct
     3061 tgcttacgat gaaaaaattg cagacgcagg ttctgtagaa tacgctaaag taaaaccaca
     3121 caaagttatc ggtgctatga aagtcttttc agacccacgc tttaacattg atgtcttgaa
     3181 agttgaagtt cctgttaaca ttaaatatgt tgaaggcttc gctgagggtg aagtggttta
     3241 cacacgtgaa gaagcagcag ccttcttcaa agcgcaagat gaagcaacga acttgccata
     3301 catctacttg agtgctggtg tatcagctaa actcttccaa gatactcttg tatttgctca
     3361 tgaatcaggt gcaaacttta acggagttct ttgtggccgt gctacatggg cagggtcagt
     3421 tgaagcttac atcaaagatg gtgaagcagc agctcgcgaa tggcttcgca caactggatt
     3481 tgaaaacatt gatgagctca ataaagttct tcaaacaaca gcgacttcat ggaaagaacg
     3541 tgtgtaagaa agtcctccta gtttaggaac atgaatctaa aaaaatttaa aaaaagttgt
     3601 atgtaaaggt ttacaaaata acttacttgt gctatactta aatcacaagt taatacaagg
     3661 tgagtgttac taagtaatat taggcatgat cacaggtgaa ttagaaatca gctgattttc
     3721 tagttcattt gtggtcattt tttgtactca tataccttta aaatataaaa ggaggttgac
     3781 atgtatcgaa ttctaaatcc aatgaatcac aatgtctcgc ttgtcagaaa tgataaggga
     3841 gaagaggtga ttgtaattgg taaggggatt gcattcggaa agaagaaggg ggatttgatt
     3901 gctgaaaatc aggttgagaa aatctttcgg atgaagaccg aagagtccag agaaaacttt
     3961 atggctcttc tcaaagatgt tccgcttgat tttatcacag tgacctatga aattattgat
     4021 aagctatcaa agaaatatca ttatccgatt caagagtatc tctatgtaac cttgacagat
     4081 catatttact gttcttatca agctctaact caaggaaggt acaaggatag taatctgcca
     4141 gatatttccg ctaagtatcc tgtcgctttt caaatcgcaa atgaagcttt tgaaatttac
     4201 cgtcagaagc tagcagatca ttttcctgag gacgaaatta ttcggattgc ttatcatttc
     4261 attaatgctg aaggtgaaaa tgaagtggaa cttgtggagt cgattgataa gaggaaagaa
     4321 attctcagga atgttgaaga agttttaacg gactatgcaa ttcaacgaac taaaaagaat
     4381 aaccatttct atgatcgctt tatgatccat ttgaattatt tcttggatta tttagacaga
     4441 tctagagatg ataaccaatc acttctggat atggaagatc atattaaaca atcctatcca
     4501 aaagccttcg agattggttc caagatctat gatgtgatta cgcaacatac gggtcttgat
     4561 ttgtataaaa gtgaacgagt ttatctagtt ctacatatcc aacgtttatt gtcataaaaa
     4621 tttatttaaa actatataag gagaattcta tcatgaatag agaagaagta acattgttag
     4681 gttttgaaat cgtagcctat gctggcgatg ctcgttcaaa actattggaa gccttgaagg
     4741 ctgctgaagc tggtgatttt gaaaaagcgg acgctctggt agaggaagct ggtagctgta
     4801 ttgcagaggc tcaccacgcg caaacaagtc tattgactaa ggaagcttct ggtgaggact
     4861 tagcgtatag tgtgaccatg atgcatggtc aagatcactt gatgacaact atcttgttaa
     4921 aagacctaat gcaccattta attgaacttt acaagagagg agttaaataa tgaataaact
     4981 aattgcattt atcgagaaag gaaagccttt ctttgaaaaa ctatctcgta atatctatct
     5041 tcgtgctatt cgtgatggtt tcattgcagg tatgcctgtt attctcttct caagtatctt
     5101 tatcttgatt gcctttgtac caaactcatg gggctttaaa tggtctgatg aagttgtagc
     5161 ctttctgatg aaaccttata gctattctat gggtattctg gctctcttgg tagctggtac
     5221 aacagctaag tcattgactg actcagtaaa ccggagcatg gaaaaaacca atcaaatcaa
     5281 ctatatgtca acattgttgg cagcaattgt tggtttgttg atgttggcag ctgatcctat
     5341 cgaaagtggt ctagctactg gattcttggg aacaaaaggt ttgctttcag ccttccttgc
     5401 tgccttcgtt actgtagcca tctataaggt ctgtgttaag aacaacgtca ctattcgtat
     5461 gcctgacgaa gttccaccaa atatctcaca agtctttaaa gatgtgattc cattcactct
     5521 atcagtggtt tctctttatg ctcttgattt actagctcgt cattttgttg gttctagtgt
     5581 ggcagaatca atcggtaaat tcttcgcacc actcttctca gcagcagacg gataccttgg
     5641 tattaccatt atctttggtg cctttgcctt cttctggttt gttgggattc atggtccatc
     5701 tatcgttgaa cctgctatcg cagcgattac ctatgcaaat gctgaagtca acttgaacct
     5761 tctccaacaa gggatgcatg cagacaagat tcttacttct ggtacacaaa tgtttatcgt
     5821 taccatgggt ggtacaggtg cgacattggt cgttccattt atgttcatgt ggttgacaaa
     5881 atcgaaacgt aaccgtgcaa tcggacgtgc ttcagtagtt cctaccttct tcggtgtaaa
     5941 tgaaccaatc ttgtttggtg cacctcttgt tttgaatcca atcttcttca ttccatttat
     6001 ctttgctcca attgcaaacg tatggatttt caaattcttt attgaaactc ttggaatgaa
     6061 ctcattcact gctaatctac catggacaac tccagctcca ctaggtctag ttcttggaac
     6121 taacttccaa gtgctatcat tcattcttgc tgcccttcta attgtggttg acgtagttat
     6181 ttattaccca ttccttaaag tctatgatga acaaattctt gaagaagaac gttcaggtaa
     6241 gtctaatgat gaattgaaag aaaaagttgc tgcaaacttc aacactgcaa aagcggatgc
     6301 tattcttgaa aaagcgggtg tcgatgcagc acaaaatacc atcactgaag aaacaaatgt
     6361 cctcgttctc tgtgctggtg gaggtacaag tggactcctt gcaaatgctt tgaataaggc
     6421 agcagcagaa tacaatgtcc ctgtgaaagc agcagcaggc ggctatggtg ctcaccgtga
     6481 aatgttgcca gagtttgatt tggttatcct tgctcctcaa gttgcttcaa actttgaaga
     6541 tatgaaggca gaaacagata agctcggtat taaacttgct aagacagaag gcgctcaata
     6601 catcaaatta actcgtgatg gaaaaggtgc cctagcattc gtacaagagc aattcgatta
     6661 aggctaggaa ctgtgaaaca gtctcccatc gttacggaaa tcgctatggc gaatttccta
     6721 atcgccttat taattcgtcg gtaaaaagat atcgttttta cctcctcatg tcacaattca
     6781 gtgacttggt acaagaagtg agatggagaa gcatggctca ctgactcctc tcctctcact
     6841 tttactttat ttaaatcaag aaataggtga aaaaaatgac aaaaacactt ccaaaagact
     6901 ttattttcgg tggcgcaaca gctgcttatc aagcagaagg tgctacacat actgatggaa
     6961 aaggaccagt tgcttgggat aaatatcttg aggataacta ctggtacact gccgaaccag
     7021 ctagtgattt ttacaatcga tatccagttg acctcaagct agcagaagag tatggtgtca
     7081 atggtattcg aatttctatt gcctggtcac gtattttccc gactggttac ggccaagtaa
     7141 atgttaaagg tgttgagttt tatcataatt tatttgcaga gtgtcacaaa cgtcatgttg
     7201 agccctttgt aactcttcat cactttgaca cgccagaagc tctccattca aatggagact
     7261 tcttaaaccg tgaaaatatc gaacattttg tagactacgc tgccttctgt tttgaagaat
     7321 ttccagaagt aaactattgg acaaccttta atgaaattgg accaatcggt gatggtcaat
     7381 atttggttgg gaaattccct ccaggtatcc agtacgacct tgccaaagtc tttcaatcac
     7441 accacaatat gatggtgtct catgcgcgtg cagtcaagct atataaagat aaaggctata
     7501 aaggggaaat tggtgttgtt cacgctctgc caaccaagta tcctttggat cctaaaaatc
     7561 cagcagatgt tcgtgcagct gagttggaag atatcatcca caataaattc atcttagacg
     7621 caacttatct aggtcgctat tcagctgaaa ccatggaagg tgtcaaccat atcttatcag
     7681 tcaatggtgg tagtttggat cttcgtgaag aagattttac aacattagaa gctgcaaaag
     7741 acttgaatga tttcctagga atcaactact atatgagtga ctggatggaa gcctttgatg
     7801 gagaaactga aattatccat aatggtaaag gtgaaaaagg aagctctaag tatcaaatca
     7861 aaggtgttgg tcgtcgtgta gctcctgact atgtaccacg cacggattgg gattggatta
     7921 tctaccctca aggtttgtat gaccaaatca tacgtgtgaa gaaagattat cctaactaca
     7981 agaagattta catcactgaa aatggtctcg gctataaaga tgagttcgtt gataacactg
     8041 tttacgatga tggtcgtatt gattacgtga aacaacactt ggagatcttg tctgatgcga
     8101 ttgcagatgg agctaatgta aaaggttact tcatttggtc attaatggat gtcttctcat
     8161 ggtcaaacgg ttatgaaaaa cgttacggtc tcttctacgt agattttgaa actcaagaac
     8221 gttatcctaa gaaatcagct cactggtata agaaagtagc ggaaactcag attatagact
     8281 agtagaatta gtcattagat atagaatttt agtgagtcaa aaagatgttc aaagatttta
     8341 tccaatctat ttatgaaaaa aagtttatat tataaatttc gaaaaatgct ctcaaatacc
     8401 gtgtttgacg agtgaagaat tgaaaagtct tggaaaatgg tatgtctcga ctggtaaaga
     8461 atggatttgt cattcagatg atgagctgga agaatttaaa aatctatttt taaattttat
     8521 caatcctgaa gaatgggata ctatctcctt tgattcagat tttatgccgt ttcaacaatc
     8581 gtaaccaatt tctcaaaaaa gttaaatctt atatttagta ctctgtaaaa ctcttatcta
     8641 atcacgttgc ttatactcaa tgaaaatcaa agagcaactt taaactagga agcgagtcgc
     8701 agatttctca atgcatagct ttgaggaatt gggcaaaaag tctttgatat agaaaaacgc
     8761 atagtatcag gtgtttcaac acctgatatt atgcgtttta ttgtgggaag atttactttt
     8821 tttcttctga aattgagttg ttacccaggc tctttcagtt tattaaggct tgatgacttt
     8881 aatgtgttta gatagcttaa aaaggattga atcacttagt ttagaatctg aaacaatagt
     8941 atcaagattt gatacattat aaaaagtata aaaatcaaac ttattgaact tgctatgatc
     9001 tgcgagtaaa tattttttat tagaattatt taaagcgatg cgttgagcct ctccctcttc
     9061 ctcgctaaaa gtagctagag ctccgttttg aataccatta cagctaacga aagctttaga
     9121 aaattggaga ttagagagat tttgtagggt caatgtacca acaaaagcac ctgtaatatc
     9181 gcgataattt ccacctatta aaatcaaatc tgttaatttt cgttcgctta aaatcagaaa
     9241 aacaggtaga ctgttggtta cgacgcggat attgtcaata ggcaactcac gcgcaaaaaa
     9301 ctctaatgtt gttcctggtc caatgaaaat agtttctctt tcttctacta gactgcctgc
     9361 aaaatgggct atttcttgtt tttctgccgt ttggagggct tgtttttcaa tatttgatcg
     9421 ctcattagtc aaaagggagt tggttcgaag tttttcagct ccaccatgca cacgaatcag
     9481 caaatcttta tcagctaatt cctgtaaata gcgccttgca gtcatatctg aaacggctat
     9541 ttcgtccata atctgtttaa ctgttatggt tcctttacta tttactatct ctaaaatttt
     9601 ggttaatttt tcttgtttga gcatattatc acctcgtttc ctactactat cttaccataa
     9661 acaaactcat cattcaaata caaaaacaac aaaatgaaac aaaaacaaaa atatcgaagt
     9721 ttgttttcaa aactttcgat atttttgttg ggttataact ttgatgtttc tagtttactt
     9781 tttgatgatt gagagtgatg gagaattagt ctaaaccgta gttatagtca tcgtcttgca
     9841 tggcttcaac ttcgccaaga aggtaaccat ttccgacttg agagaagaag tcatggttgg
     9901 aagttcctgt tgaaataccg ttcataacga ttgggttgac atcttcagct gaatctggga
     9961 aaagtggatc ttgtcccatg ttcatgagag ctttattggc attgtagcga aggaaggttt
    10021 taacctcttc agtccaacca acaccgtcat aaagactctc tgtgtagcct tcttcatttt
    10081 cataaagagt atagagtagg tcgtacatcc attctttaag tttttcttgc tcttcttcag
    10141 gcaattcatt gaaaccaagt tggaatttgt aaccaatgta ggttccgtga acagactcgt
    10201 cacgaataat caatttaatg atttctgcaa cgttggctag tttgttgtta ccgagatagt
    10261 agaggggagt gaagaaacca gagtagaaga ggaaggtttc gaggaagacg ctggcaactt
    10321 tcttttcaag tgggctgccg tttaggtaga tttcgttgac aatctcagcc ttcttttgta
    10381 ggtaaggatt ggtattggtc cattcgaaaa tttcttcaat ctcagcctta gtattcaagg
    10441 tagaaaagat tgatgagtaa gatttagcgt ggacagattc cataaattgg atgttattga
    10501 aaacagcttc ctcatgtggt gtacggatgt ctgcgcgaag ggcttgaacc ccagtttcag
    10561 attgcatagt gtcaagaagg gttaaaccac caaaaacttt tccgaccaag tctttctctt
    10621 tgttagatag ctttctccag tcatccaagt cgtttgataa gggaatacgt gtatcgagcc
    10681 aaaattgctc cgtcagtttt tcccaagttg atttgtcgat gacatcttcg atggcattcc
    10741 agttaatggc tttgtagtaa gtttccattt aaaatctctt tctgtgttta gtattgcgaa
    10801 ctcacaatta tttctacttt accataattc tataggagta tcgcacaaaa agtcggaagc
    10861 ccgactttta aaatgttaca taaattatgt tatgacatag tagatttgat tttatcagtg
    10921 ctgcttaggg aaaaataatg tttctatgct agaaactaaa tcacacagct ttcacattgg
    10981 ttggcgccga cttctccacc gtcatctgta aaggtacgga cgtagtagat agacttgatt
    11041 cccttgttaa aggcatagtt acgaaggatg gacaaatcac gtgtcgtttg tttattttcc
    11101 ctcttccatt cgtaaaggcc ttttggaatg tcactgcgca tgaagagggt gagtgaaagt
    11161 ccttgatcca cgtgttcagt cgcagcagcg taaacatcga tgactttacg catatccata
    11221 tcgtaggcag aagtgtagta aggaatggtt tctgtagaca agccagcagc agggtagtag
    11281 attttaccaa ttttcttctc ttggcgttct tcgatacgtt gcgtaatcgg gtggatagaa
    11341 gcagaaacgt cgttgatata gctgatagaa ccatttggcg ctacagcaag gcgattttgg
    11401 tggtaaagac catcttcttg aaccttgtcg cgaagttcag cccaatcagc aacaccaggg
    11461 ataaaaacat ttttgaagag ttctttaaca cggtctgatg ttggaacaaa ttcaccagtt
    11521 acatacttgt caaagtaact tccgttagca tagtctgatt tttcaaagtt gtggaaggta
    11581 ataccacgtt cacgcgcaat attgtttgac tctaccaagg tccagtagtt cataagcata
    11641 aagtagatgc ttgtaaattc aacagactca ggtgaaccat attcaatgag ttgttgggca
    11701 aggtagctgt gcagtcccat ggcaccgaga ccaaaggtgt gggcttggct atttccatgg
    11761 tcaatcgttg gtacagctac gatatgtgaa ctatctgtaa cgaaagtaag ggcacgaacc
    11821 atagcacgga tagaacgacc aaaatcaggt gaagtcatca tgttaaccac gttggttgaa
    11881 cccagattac atgaaacatc tgttcccatt tgaaggaatt cttgagcatc gttgatcaag
    11941 cttggttctt gaacttgaag aatctcagaa cacaagttac tcatgataat ctttccatca
    12001 acaggatttg cacggttagc cgtatcgatg ttgactacat aaggatagcc agactcttgt
    12061 tgcaatttag agatttcagt ttccaaatcc cgcgccttga tttttgtctt gcgaatattt
    12121 ggatttgcga ccaattcatc gtatttttca gtaatgtcga tgtaattgaa tggcacaccg
    12181 tattcttttt ctacagagta agggctgaag aggtacattt cttcattttt acgagccaat
    12241 tcgtagaatt tatcaggtac tacaacacca agtgatagag tcttgacacg tactttttca
    12301 tcagcgtttt ctttcttagt tgaaaggaaa gcgatgatat ctgggtgaaa gacgttgagg
    12361 tagacaacac cagcaccttg acgttgcccc aattggttgg agtaagagaa gctgtcttca
    12421 aaaagcttca taacaggaac gacacctgaa gcagctcctt catagccttt gataggtgca
    12481 ccagcttcac gaaggttgct gagggtaatt cccacaccac caccaatacg tgaaagttga
    12541 agagctgagt tgatagaacg tccgatagag ttcatatcat ccgtcacttg gattaggaaa
    12601 caagatacca actccccacg acgagcacgt ccagcattca agaaggaagg agtagcaggt
    12661 tggtagcgtt ggtggatgat ttcattggca atatcgattg caacagcttc attcccatca
    12721 gcgaaataaa gggcgttgaa gaagacacgg tcttccatat tttcaagata gtattcaccg
    12781 tcattagtct ttaaggcata ttgattgtaa aatttataag ctgccatgaa tgacttgaat
    12841 tggaagtttt ggtctttgat aaattgagcc aattcttcca agaactctgg acggtatttc
    12901 ttgataaagg ctgtttcgat gtagttgtgt tcaatgaggt aattgatttt gtctttgatt
    12961 gaatcaaaaa ccatagtgtt tggaactaca ttttctttaa agaaagcatc caaggcttcc
    13021 ttatctttat gaagcatgat ttgtccatta acaggacggt taatttcgtt attaagacgg
    13081 aagtaagtca cgtcttcaag atgttttaat cccataaaat ttcccttatc taattacaaa
    13141 agaaaggctt ctaagttagc cctaaaagca gtttcttctg gatgatgtac taagattatg
    13201 ctaattgttt cagttttcct ggttggaaac ctgaaaagac ttcagttggt gtttggataa
    13261 caggagctgc gctaaaaccg agctctttaa cttgatcgac gtactcaggt tgctcatcaa
    13321 gattgatttc acgataagag acattattac tgtccaagaa acgcttggtc attttacatt
    13381 ggacacaatt gtttttagaa taaacggtta ccattgtgta actcctcttc aaaatttaat
    13441 actatcttag tatatcagaa aataaaattt tgtcaatgat ttgaactaaa tatagtggtg
    13501 ttcttttgtt gtttttatat ataaacacaa tatatagtgt ttgtattgtg aaatagcaat
    13561 gattatccta taaattgttt ttaaagaaac tatatcctgt gttttgttat ctaggataga
    13621 agattttcag caagttatct gaaaggaaat tgaaggaaaa tccagaaaat gcttgaaaaa
    13681 aatcctagaa gatggtataa tactaaattg taagggttat cacatataac tcaaaaaaag
    13741 aaagaacaaa aggagagtca aactatggct tctaaagatt tccacgtagt ggcagaaaca
    13801 ggtattcacg cacgtccagc aacattgttg gtacaaactg ctagcaaatt tgcttcagat
    13861 atcactcttg agtacaaagg taaatcagtt aaccttaaat caattatggg tgttatgagt
    13921 cttggtgttg gtcaaggtgc tgacgtaact atctcagctg aaggtgcaga tgcagatgac
    13981 gctatcgctg caatctcaga aacaatggaa aaagaaggat tggcataagg gaaatgacag
    14041 aaatgcttaa aggaatcgca gcatctgacg gtgttgcagt tgcaaaagca tatctactcg
    14101 ttcagccgga tttgtcattt gagactatta cagtcgaaga tacaaacgca gaagaagctc
    14161 gccttgatgc cgctctacag gcatcacaag acgagctttc tgttattcgc gagaaagcag
    14221 taggtacgct cggtgaagaa gcagctcaag tttttgatgc tcacttaatg gttcttgctg
    14281 acccagaaat gatcagccaa atcaaggaaa ctatccgtgc gaagaaagtg aatgcagaag
    14341 caggtctgaa agaagttaca gatatgttta tcactatctt tgaaggcatg gaagacaacc
    14401 catacatgca agaacgcgca gcggatatcc gcgacgtgac aaaacgtgta ttggcaaacc
    14461 ttcttggtaa aaaattgcca aacccagctt ctatcaatga agaagtgatt gtgattgcgc
    14521 atgacttgac tccttcagat acagctcaat tggacaaaaa ctttgtaaaa gcttttgtaa
    14581 ccaacattgg tggacgtaca agccactcag ctatcatggc acgtacactt gaaattgctg
    14641 ctgtattagg tacaaacaac atcactgaaa tcgttaaaga cggtgacatc cttgctgtta
    14701 acgggatcac tggagaagtg attatcaacc caacagatga acaagcggca gaatttaaag
    14761 cagctggtga agcctatgcg aaacaaaaag ctgaatgggc acttttgaaa gatgctcaaa
    14821 cagtgactgc tgacggtaaa cacttcgagt tggctgccaa tattggtact ccaaaagacg
    14881 ttgaaggtgt taacaacaac ggtgcagaag ctgttggact ttaccgtaca gagttcttgt
    14941 acatggattc tcaagacttc ccaactgaag atgagcagta tgaagcatac aaggctgttc
    15001 ttgaaggaat gaacggtaaa cctgttgtcg ttcgtacaat ggatatcggt ggagataagg
    15061 aacttcctta cttcgatatg cctcacgaaa tgaacccatt ccttggattc cgtgctcttc
    15121 gtatctctat ctctgagact ggagatgcta tgttccgcac acaaatccgt gctcttcttc
    15181 gtgcgtctgt tcacggtcaa ttgcgtatca tgttcccaat ggttgcgctc ttgaaagaat
    15241 tccgtgcagc gaaagcagtc tttgatgaag aaaaagcaaa ccttcttgct gaaggtgttg
    15301 cagttgcgga taacattcaa gttggtatca tgatcgagat tcctgcagcg gctatgcttg
    15361 cagaccaatt tgctaaagaa gttgacttct tctcaattgg tacaaatgac ttgatccaat
    15421 atacaatggc agcagaccgt atgaacgaac aagtttcata cctttaccaa ccatacaacc
    15481 catcaatcct acgcttgatt aacaatgtga tcaaagcagc tcacgctgaa ggtaaatggg
    15541 ctggtatgtg tggtgagatg gctggtgacc aacaagctgt tccacttctt gtcggaatgg
    15601 gcttggatga gttctctatg tcagcaacat ctgtacttcg tacacgcagc ttgatgaaga
    15661 aactcgacac agctaagatg gaagagtacg caaaccgtgc ccttacagaa tgctcaacaa
    15721 tggaagaagt tcttgaactt caaaaagaat acgttaattt tgattaatcg aaaagtccct
    15781 gcaactcagt tacagggact ttttttgata ttttaaaaag aattttcaag aaaatctttc
    15841 ttataaaaag tccaaccttg aaaaagtagt ggtcagaaca aaaaatactt aaatggttca
    15901 taaaattctt gacaagttgg agatttagga gtaaactatt aaccagttaa gtaatagaga
    15961 ggagtttctg caatttagaa atgaattgca actagaaata tcaaatagaa agagagtttc
    16021 gatgaaaatt aataagaaat accttgttgg ttctgcggca gctttgattt taagtgtttg
    16081 ttcttacgag ttgggactgt atcaagctag aacggttaag gaaaataatc gtgtttccta
    16141 tatagatgga aaacaagcga cgcaaaaaac ggagaatttg actcctgatg aggttagcaa
    16201 gcgtgaagga atcaatgctg agcaaatcgt catcaagata acagaccaag gctatgtcac
    16261 ttcacatggc gaccactatc attattacaa tggtaaggtt ccttatgatg cgattttcag
    16321 tgaagaatta ctcatgaaag atccaaacta taagctaaaa gatgaggata ttgttaatga
    16381 ggtcaagggt ggatatgtta tcaaggtaga tggaaaatac tatgtttacc ttaaggatgc
    16441 tgcccacgcg gataacgtcc gtacaaaaga ggaaatcaat cgacaaaaac aagaacatag
    16501 tcaacatcgt gaaggtggga ctccaagaaa cgatggtgct gttgccttgg cacgttcgca
    16561 aggacgctat actacagatg atggttatat ctttaatgct tctgatatca tagaggatac
    16621 tggtgatgct tatatcgttc ctcatggaga tcattaccat tacattccta agaatgagtt
    16681 atcagctagc gagttggctg ctgca
//
LOCUS       DAVBDG010000043        15834 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBDG010000043 DAVBDG010000000
VERSION     DAVBDG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.044"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(356..748)
                     /gene="rpsI"
                     /locus_tag="VQ526_001448"
     CDS             complement(356..748)
                     /gene="rpsI"
                     /locus_tag="VQ526_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2555160.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(767..1213)
                     /gene="rplM"
                     /locus_tag="VQ526_001449"
     CDS             complement(767..1213)
                     /gene="rplM"
                     /locus_tag="VQ526_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2555161.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1569..1886
                     /locus_tag="VQ526_001450"
     CDS             1569..1886
                     /locus_tag="VQ526_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2555162.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1931..2743)
                     /gene="folK"
                     /locus_tag="VQ526_001451"
     CDS             complement(1931..2743)
                     /gene="folK"
                     /locus_tag="VQ526_001451"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2555163.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILFYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLET
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2786..3340)
                     /gene="folE"
                     /locus_tag="VQ526_001452"
     CDS             complement(2786..3340)
                     /gene="folE"
                     /locus_tag="VQ526_001452"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2555164.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3321..4643)
                     /locus_tag="VQ526_001453"
     CDS             complement(3321..4643)
                     /locus_tag="VQ526_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2555165.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFNSPYLIHYTDQISINGESISEARLEALMADY
                     QSLMGGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEEKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTIRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4645..5625)
                     /gene="folP"
                     /locus_tag="VQ526_001454"
     CDS             complement(4645..5625)
                     /gene="folP"
                     /locus_tag="VQ526_001454"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2555166.1"
                     /translation="MMKSLEGVTDMSSKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTRKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSS
                     LIFPHFGFNQAFTVEELADFETLPIEDLMVAFFERALARAAEAGIAQENILLDPGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5870..6577)
                     /locus_tag="VQ526_001455"
     CDS             complement(5870..6577)
                     /locus_tag="VQ526_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2555167.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSIVLSWTAYKTQRLEMSILLHMIVNGVAF
                     CLLALVVILSRTLGISV"
     gene            complement(6630..8048)
                     /locus_tag="VQ526_001456"
     CDS             complement(6630..8048)
                     /locus_tag="VQ526_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2555168.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            8238..9050
                     /locus_tag="VQ526_001457"
     CDS             8238..9050
                     /locus_tag="VQ526_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2555169.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9389..10408
                     /gene="adhP"
                     /locus_tag="VQ526_001458"
     CDS             9389..10408
                     /gene="adhP"
                     /locus_tag="VQ526_001458"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2555170.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            10641..11630
                     /gene="manX"
                     /locus_tag="VQ526_001459"
     CDS             10641..11630
                     /gene="manX"
                     /locus_tag="VQ526_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2555171.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            11658..12461
                     /locus_tag="VQ526_001460"
     CDS             11658..12461
                     /locus_tag="VQ526_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2555172.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            12485..13396
                     /locus_tag="VQ526_001461"
     CDS             12485..13396
                     /locus_tag="VQ526_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2555173.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(13452..14786)
                     /gene="pepC"
                     /locus_tag="VQ526_001462"
     CDS             complement(13452..14786)
                     /gene="pepC"
                     /locus_tag="VQ526_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2555174.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(15004..15726)
                     /locus_tag="VQ526_001463"
     CDS             complement(15004..15726)
                     /locus_tag="VQ526_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2555175.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILLANII"
BASE COUNT         4562 a         3560 c         3092 g         4620 t
ORIGIN      
        1 agagttctaa cttacccatt ctatggaatc ttgcattatc cataatatag tagattgaaa
       61 ctagaatagt acacctctac ttctaaaaca ttgttagaat tcgatttgac tatcctcagt
      121 ttgtcctatt cttatttcac attactataa aaataaggta aacttttgag tgctttgaaa
      181 cgttgatata atcgtattga ttaacgtttt gaggcaactg accatgtcag gttgcagcca
      241 catttgtaag cgactaaagt cgcaacaact gtgtcaattg caccaattta gtaagaaagt
      301 ataaaaaaag aacaccccga aaggtgctct gtataagtat agtaattctt tcgaattaac
      361 gtttactaaa ttgtgatgct ttacgagctt tcttaagacc tggtttctta cgttcaactt
      421 tacgtgagtc acgtgtaaga agtcctgcgc gtttcaatga atcgcggaag tctgggtcta
      481 cttgaagaag ggcacgagcg ataccatgac ggatagctcc tgattgacca gcgtatccac
      541 cacctataac gttaacgaaa acgtcgtatg aacctacagt tgaagtaact gcgaatggtt
      601 ggttgatgac aagacgaagg tcagcgtgtg ggatgtactc ttcaacatct tttttgttaa
      661 cagtgatttt accagttcct ggaacaaggc gaacgcgtgc aacagcgttt ttacgacgtc
      721 cagtacctgc atattgtgct tgtgacatac tttattgttc ctttccttag ataagtcctg
      781 aaatgtcaag aacttctggt tgttgtgcag catgagtgtg ctcagctcca acaaatactt
      841 tcaacttcat accttgagcg cgtccaagtg tattgtgtgg aagcatacct ttaactgatt
      901 tctcaatcaa acgtactgca tttttagaac gaagttcacc tgcagagatt tgtttcaatc
      961 cacctgggtg gtttgagtga gtgtagtaga ttttatcagt tgctttttta ccagtcaatt
     1021 taactttttc agcattgata acaatcacga agtcacctgt atcagtgtgt ggtgtaaatg
     1081 ttggtttgtt ttttccgcga agtacgctag caactactgc agaaagacgt ccaagtggta
     1141 catcagttgc gtcaactacg taccatttac gttcaacttg gcctggttta gccataaatg
     1201 ttgttttgtt catgatttct cctatatata gttcgatttt tgtttacggt gatgggtgtt
     1261 ccttcccatc gaaaagtatt tggaaggttc cggggccttt caaatggggt aaacaatacc
     1321 gcctactatc ataccaaatt tcacccctaa aagtcaatag atatgaaaaa tatttttcgg
     1381 taacttctca aagtaactga aagttatata atcaagttaa tttgggaatt tacctattaa
     1441 cttcggtaga tttttgggaa ttttttccct atttctccct gtagctaaaa tccttttcaa
     1501 aaaatcacct tttcttgatt tttaatccta tttgctgtat gataagggaa aagaaagggg
     1561 acagagatat ggcttttacc aatacccaca tgcggtctgc tagttttggc attgttacca
     1621 gcttgcctga tgacatcatt gactcttttt ggtatatcat agaccatttc ttaaaaaatg
     1681 tctttgaatt ggaagaagaa ctcgagtttc aattgcttaa taaccaagga aagattacct
     1741 tccacttttc aagtcaacac ctccctacag ccattgattt tgactttaac catcctttcg
     1801 accctcgtta tcccccaaga gtactggttt tagacatgga cggtagagaa actatcctcc
     1861 tcccagaaga aaatgaccta ttttaaaaac tctagccttc agttgcaagt aactgaaaac
     1921 tagagttttt ctattttttc aaagcatcat acaagttgcg gatcggttgt tttaatatcg
     1981 gatggataaa atgaggcgca atttcctgta aggactcaag gacaaaaagg cgttccgcta
     2041 tgtaaggatg aggcaatatg aggtcgtctg tataaaggat ctggtcctcc acaaagagca
     2101 agtccaaatc aatcaaacga ggtccccaat gcacttctct cacccgtccc agctctgact
     2161 caatggctaa caaggtttct aacaagtctt gtgctggtag ccaggtttcc acctcaacca
     2221 cttgattggc aaagctatcc tgctccactc caccccaagg ctccgtctct aagacactgg
     2281 actctttgag aatatggatg ccacgagctc gcagtttgtc aatggcttgc ttcaagttta
     2341 cttgtttatc tcccatattg cttcctaggg cgataaaggc tcgttgcttg cggcgatgaa
     2401 tggttaccga gcaagtatct agtgacaaat gcaccggtgc ccaaggtttt ttcagttcca
     2461 acttcatttc ttggacaaga ggataagact caaaggtacg ttccaccagt ttataggcta
     2521 ccgtttcaat caaatcttca ctcgtttcct gaaaccaagt cgtccactgc tgacacaatt
     2581 ctccgtaatg gacagaggct gttaaatcca agtctgtagc tgccttggtc atatcataga
     2641 ataggatggc tgaaacgaca aatttctgcc ccaattcttt ctcactagga aaaagaccat
     2701 gataggcaaa catttccaaa tccttaatct gcagttgatc cataatgatt cctttctaga
     2761 aaaatctgct tgaagcggat tctttttata gccccattaa acgataagct tggtcacgga
     2821 gatccttatc tgtttcaaat agaccacgag ctactgtcgt caaggttgca gtgcctggtt
     2881 ttctaacacc acgcatactc atacacatat gttccgcctc aatgacaaca aaggctcctt
     2941 tagcacctag atagtccatc aaggcatcgg ccacttcgat attcaaacgt tcttgaattt
     3001 gtggtttttt cgaataaact tcaaccgtac gggctagctt agacaagcct gccacacgac
     3061 catctggaat gtaggcaatg tgcgctctac cataaaatgg caagaagtgg tgttcacaca
     3121 tggtatggaa aaagatatcc ttttctacca ccatattatc gtcaataatt tcaaaggatt
     3181 ttgacaaatg ttcctctgct gtttgaccaa gacctgaaaa aatctcttga tacatacggg
     3241 ctacacgagc aggtgtttcc tgcaagccct cgcgattagc gtcctctcct acagcctcga
     3301 taatcatttt tacagctgct tcaatctttt gtgtatccat tctcgttctt cctctccatc
     3361 agataggccc tcacttggct caagaaatac aaggaacctg tgacaatcct aattgtttgt
     3421 ttctcttctt ttttatctgt caaattctgc tctagaaaat catgccaatc ttggtagctg
     3481 agatttctag acttagctgc ctctttcagc acgctttcat ccgtcgcccg actatccgca
     3541 aaatgtgtta gagtaagctc ggtatctggc atggctccca gcaagtccaa catatcctcc
     3601 aaggccttgg ttttgataca agtgaagagg atttccttat gataatccgc aaaacgttct
     3661 tgcaaggtta ccaacaaggc cttgatagca tggggattgt gggctccatc caaaatcatc
     3721 aagggatctc ttgacacgat ttccaaacgc cctggccaac ttgtttcttc caaggcttga
     3781 ccaagaaagt cattgcttgc tagctctcga ccatcttctt gacaaaaagt atcaagtaaa
     3841 gctatggcca tcccagcatt ctctatttgg tacaaaccaa gcaggctagt ctggaagcga
     3901 ccttgtctga cagcacttgt atagtcaaag acttcccctg tcaccacact ttcttgatga
     3961 cgaacctgat aatctgtccc gtaggcaagt ctcggcgcat ctttctcttc cgcaatgcgg
     4021 tcaatcacag tcaaggcttc tggagcaata cgccctgtta ccaagggcat cccttgtttg
     4081 ataatacctg ccttctgctc tgctatggcc tccaaggtgt caccaagtag ggccacatga
     4141 tccaagccaa tagttgtaat tcctgtcaaa atgggctgac agacattggt actatccaaa
     4201 agtccaccca tgccaacttc catgatggcc acatctactt gctctgaggc aaagtagtca
     4261 taggccaggg ctgtgataat ctcaaactcg gttgtgccct gtaaattggc gaccgcttct
     4321 ccccccatca aagactgata gtctgccatg agagcttcta gcctcgcttc tgagatcgat
     4381 tccccattga tgctaatctg gtctgtgtaa tgaatgagat agggcgagtt aaacacgcca
     4441 actctcagcc ctagcttttc tagcatcttt ttcaaaaaag caatagtcga gcccttgccg
     4501 ttagtccctc cgatatggag gaccttgagt ttgagatggg gattgccacg caaagctaac
     4561 agttccacca ttcgttccaa tccaaaatgc ggttgatccg tccggtagtt agcaatccac
     4621 tgattgtttt caatttcttt catcttattt atattgtttt aaatctaaat tttccgcttc
     4681 atcagccaga cgaatagcgg aagcaatttc aactgccatc ctgtgactag ctacgtcatg
     4741 cacgcgcacc acttctacac cctgtctcgc agcgatactt gttacatgag ccgaagccgt
     4801 gtcccgattt cggaaaccaa gttctgtctc aggattgact tcaaacccac tttcttcaag
     4861 gatattgatg acaaaccgct tgcgcgacac tccaagaaag attggatacc ccttctgatg
     4921 tagtttatcc aagtcccgta aaagaagcag attttctttc ttggtcagac caaagccgat
     4981 tcccggatcc aacaggatat tttcttgtgc aataccagct tctgccgctc tcgctagtgc
     5041 tcgttcaaag aaagccacca tcaagtcttc gattggcaat gtttcaaagt cagctaactc
     5101 ttccactgta aaagcttgat taaatccaaa atgaggaaat ataagcgagc taggatgctg
     5161 aggtcgcgcc atgactggat taaacataat gactactttc gcaccagcct tagcaaccac
     5221 atgggccatt ttctcatcac ccataagacc agtgatatca ttgactagat tggcaccagc
     5281 agccaaagca gcctctgcca cctgactctt ccgagtatca atagagatga ggacatcact
     5341 ttccttgcga atcgctttaa tcactggaac aacacgctgg atttcctctt ctatctcaac
     5401 atagctactt cccggccgag gccgagtcga ttctccgccg atatctagca tactagcccc
     5461 ttctgctatc aatttacgag cctgctggag tgcctgctca agagcaaaaa attgaccacc
     5521 atccgaaaag gaatctgggg ttacattgat aattccgcaa atagctgtct ttgcatgatt
     5581 ggctttactt gacatatcgg tcactccctc aaggcttttc atcatattat ttctctattt
     5641 taccataaaa agaaaaagat ggacacaatt gcattcatct ttttcccagt agaagcaagt
     5701 aagcaattgt caataatctt atactcttcg aaaatctctt caaaccgcat caacgtcgcc
     5761 ttgccgtata tatgtgactg acttcgtcag tcttatctac aacctcaaaa cagtgttttg
     5821 agcagcctgc ggctagtttc ctagtttgct ctttgatttt cattgagtat taaacagaaa
     5881 tccctaatgt ccgactcaaa atcaccacga gagccagcaa acagaaagca accccattca
     5941 caatcatgtg aagtaagata gacatttcca aacgttgggt cttgtaagcc gtccaagata
     6001 gaacgatcga catacctcca taaatcaata aagaaggtaa attacttggt tgatgcaata
     6061 aagcaaacac aatcgtaccg actacaaatc ccaagttctc cttacctcgg aaaatctttt
     6121 taggaacaat cccacggcac aagatttcct cacaaattgg agcaagcaag gctagcaaga
     6181 agaagctaga aatcagagaa ctattctgaa ccatatcgtt aatctgagac tggttagctg
     6241 ttgtcgtctc atttgacagt tgcaacaaga tagaaccaag tatatttgaa cagataatga
     6301 ctaaataact caagcccaaa cgtgccaaat ccttagttct aaaaaaagaa aaattaaaac
     6361 ttgccaattg cgttttacga gctccaataa taaatagcac cagaaccaca atagaaatac
     6421 cagtaactat cagccctgac tgtagcaggg ctacttcttt taaagtcaaa atagaggtaa
     6481 ccactagggg aacctgatat aaaaggacgg caagtaaaaa gaccaaaatc cagcctgcac
     6541 gattcaacaa ttctttccac atatttttct ctttcatcac ctatctcctt tgtattaaaa
     6601 aaggggggaa atccccctgg gtcattctat tataaggcca tgctgatgta gttaaggata
     6661 aacaaggcat ccaaaatcca aatcatgaca tgaacatctt taacttgacc tttaacaagc
     6721 ttagtcaaag tgtaagtcaa gaaaccaact gcaatccctt gagtgataga gtagctgaat
     6781 cccataaaga tagatgtgaa gaaggcagga actgcttcag acatatcatc ccaatggata
     6841 tttttcaagc taccaagcat cataatccca acgataatca agattggagc tgtagccgct
     6901 gttggtacga tcgctagaag tgggctaaag aagcttgaaa tcgcaaaaca gatagctaca
     6961 accaaggctg tcaaaccagt acgtccacca gcaccgatac cagcagcaga ctcaacataa
     7021 gtcgttacgt ttgaagtacc tgcaatggca ccgactgtcg ttccaatcaa atcagagtaa
     7081 agagccttat ccaatttggc tgattggtga ttttcaccat ttgtcgctac gataccaact
     7141 ttttcacctg taccgatcaa ggtaccaatt gtgtcaaaaa tatctgtcaa tgagaaggca
     7201 agaatggcca tcagagtttc aggcaagcga gctgtatctg aaaccaaagc tcccaatcct
     7261 tctgaaccaa gagctgcacc aaagattgtc ttcaaatctt caaaagctgc accaacatgg
     7321 ttattagcaa aatcgatact agacaaatca accaaaccaa ctgcaatagc aagaacagtt
     7381 gttgtcaaga tagagagaat aattccccct ttaatccctt tgatgacaaa gaagatagta
     7441 atggcaagtc ctgcaagagc cactaaaaca gctggattat taaagctgac caatcctgga
     7501 actgctgaag agtttgctgc aatcgttgct tgagctttgt cagccccttc tcctacaaca
     7561 gtatagttgc ctggatcaat cgtgaatttc aaaagtccag cattcttaat ccctacatag
     7621 gcaaggaaga caccgatacc agctgaaata gctgagcgaa gagcattggg aatcgattca
     7681 atgatcattt tacgaacatt tgtcaaggta ataatcaatg aaataatccc acagatgaag
     7741 accatagcta gggcttcttg ccaagaataa ccaagcccga atacaactgt aaaggtaaag
     7801 aaggcattga gtcccatacc tggcgcttgg gcataaggta agttagcata aaaagccatc
     7861 atcagggtac ccgctactgc accaataatc gtcgctagga agacgccctg agcaggcatt
     7921 cctgtttgtg aaagtatttg tgggtttaca aagagaatat agctcattgc aaagaaagtt
     7981 gttaaaccag cgagaacctc tgtacgaacg tctgtaccgt tctcttttag tttaaataat
     8041 ttgtccatca tcaatctcct tttaattttt acgaacaata atagaattat atcatttatc
     8101 attcactttt tcaatgtctt tgtttgattt gtttaagaaa ttaatccaat ttcatttttt
     8161 gtttggaatc tgcttttctg cctgctttct gttataatag aagacatctt atctgaaaac
     8221 acactagaaa ggtccatatg acgaaaaaaa ttattgcagt tgacctggat ggaaccctgc
     8281 tcaactcaga cagtcaaatt tctgacttta ccaaaaaaac catcaaaaaa gttgctgaaa
     8341 aaggccatca ggttattatt acgacaggtc gcccttaccg tatgtcaaaa gatttttacc
     8401 gtgaactggg cttagacact cctatgatta acttcaacgg atcccttact catttaccag
     8461 accaagtttg ggattttgaa aagtgtttga ctgtagacaa aaaatatctg ctagatatgg
     8521 ttcaacgttc agaggacatt caagccgatt ttatcgctgg agaatatcgt aaaaaattct
     8581 acattacaaa tcccaatgaa gaaattgcca atcccaaact atttggtgta gaagctttcc
     8641 agcctgaaga tcaattccag cctgaattgg tgaccaagga ccctaactgt atcctcttgc
     8701 agaccagagc cagtgacaaa tatgccttgg caaaagacat gaacgccttc taccagcatc
     8761 aactttctat caatacctgg ggaggtccgc tcaatatcct tgaatgtacc ccaaaaggtg
     8821 tcaacaaggc ctttgctttg gactacttgc tcaagataat gaatcgtgac aaaaaagatt
     8881 tgattgcctt tggagatgaa cacaatgata ccgaaatgct cgcttttgct gggaagggtt
     8941 atgccatgaa aaatgccaat ccagagctac tcccttatgc agatgagcaa atttccctta
     9001 ccaacgacca agatggggtt gccaaaaccc tacaagactt attcttataa cctatactga
     9061 tactcaatga gaatcaaaga gcaaactagg aagctagccg taggttgctc aaagcactgc
     9121 tttgaggttg tagatgaagc tgacgtggtt tgaagagatt ttcgaagagt atgaccagct
     9181 tgcaagctgg tctttgtgct cttttcacag tctcatcaac cagttaatcg attgttctac
     9241 tttttgtctc caaaaccttg gaaaaggctt tcttttgtga tatacttcac atataaatac
     9301 aagagagctc gtcacactcg actctgttga caaaaaatca atccagtccc gttgtccctg
     9361 ttctcggtca atatcaagga ggatagctat gaaagctgtt gttgtaaatc cagaaagcac
     9421 tggtgttgct attgaagaaa aagtactccg tccacttgaa actggggaag cacttgtaga
     9481 agttgaatac tgtggtgttt gccacaccga cctccacgtt gctcatggtg actttggtca
     9541 agtcccagga cgtgttcttg ggcacgaagg tatcggtatc gttaaagaga ttgctccaga
     9601 tgtgaaaagc cttaaagtcg gtgaccgcgt cagcgttgct tggttctttg aaggatgtgg
     9661 cacttgcgaa tactgtacaa ctggtcgcga aaccctttgc cgtacagtga aaaatgctgg
     9721 ctactcagta gacggtggta tggctgaaca atgtatcgta actgctgact atgctgtcaa
     9781 agttcctgac ggacttgatc cagcccaagc ttcttctatc acatgtgctg gagtaacaac
     9841 ctataaagct atcaaagaag caaaagttga accaggccaa tgggttgttc tttacggtgc
     9901 tggtggactt ggtaacctcg ctgttcaata cgctaaaaaa gtattcaatg ctcatgttat
     9961 cgcagtcgat atcaacaatg acaaacttac ccttgcaaaa gaagtaggcg ctgacattgt
    10021 gattaacggc ctcgaagttg aagatgtagc tggactcatt aaagaaaaaa ctgatggagg
    10081 agctcattca gctgtcgtaa ctgctgtgtc taaagttgcc ttcaaccagg ctgttgactc
    10141 cattcgtgct ggtggtcgcg tcgtcgctgt tggtcttcct tctgaaatga tggaactcag
    10201 catcgttaaa acagtcctcg atggaatcca agtcatcggt tctcttgtcg gaactcgtaa
    10261 agacttagaa gaagccttcc aatttggtgc agaaggtctg gtagtcccag ttgttcaaaa
    10321 acgtccagta gaagatgctg ttgccatttt cgacgaaatg gaaaaaggcc aaatccaagg
    10381 acgtatggta ctcgacttca cccactaatc taatagaaac ctattttaaa agttggaagc
    10441 ttcttccaac ttttttatat taaattttaa ataagaattc aagattactc ctcaaaaact
    10501 caacatatca acatacaaaa ataaaaattt tttaattttt tatgataaat agttgctttt
    10561 tttatgaaaa cggtttatac ttaaacagtc ttaaagtttt cttaaatgaa aactaaaaca
    10621 aaaaaggagg aatgacaata atgagtatcg gaatcattat tgcgagccac ggcgaatttg
    10681 ctgcgggtat tcatcagtca ggatctatga tctttggtga acaagaaaag gttcaagttg
    10741 tgacctttat gccaaatgaa ggtcctgatg atctatacgc taagtttaat aacgctgttg
    10801 ctgcatttga cgcagaagat gaggttctag ttttggctga cctttggagt ggttctccat
    10861 ttaaccaagc tagtcgcgtg atgggagaaa atcctgagcg taagtttgcc atcatcacag
    10921 gacttaactt accgatgttg attcaagcct acacagagcg cctcatggac gctgctgcag
    10981 gtgtagaaaa agtcgctgct aatatcatta aagaagccaa agatggcatc aaagctcttc
    11041 cagaagagct aaatccagtt gaagaagttg caagcgctgc agctgctcca gttgcccaaa
    11101 ctgctatccc agaaggaact gttatcggag acggtaaatt gaaaatcaat cttgcccgtc
    11161 ttgacacacg tctacttcac ggtcaggttg caactgcttg gactccagat tcaaaagcaa
    11221 atcgtatcat cgttgcttca gataacgtgg ctaaagacga ccttcgtaaa gaattgatta
    11281 aacaagcagc tccaggtaat gtcaaggcta atgtggttcc aattcaaaaa ctgattgaga
    11341 tttcaaaaga cccacgtttt ggagaaacac atgcccttat cttgtttgaa acacctcaag
    11401 atgcccttcg tgccatcgaa ggcggcgtgc caatcaagac tcttaacgtt ggttctatgg
    11461 ctcactcaac aggtaaaaca ttggtcaata ccgttttgtc tatggacaaa gaagatgttg
    11521 ctacatttga aaaaatgcgt gacttgggtg ttgaatttga tgtccgtaaa gtaccaaatg
    11581 attctaaaaa agatttgttt gacttgatta acaaagccaa tgtcaaataa gccattattt
    11641 atgaaaggat tttaaacatg tctattattt ctatggtttt agtagtcgtt gtagccttct
    11701 ttgcaggtct tgaaggcatc ctcgaccagt tccaatttca ccaaccactt gtagcctgta
    11761 cccttattgg gcttgtaaca ggtcacttgg aagcagggat tatcctcggt ggatcgcttc
    11821 aaatgattgc ccttggttgg tcaaatatcg gtgctgctat cgctcctgat gctgcacttg
    11881 cttctgtcgc tgctgccatt atcatggttc ttggtggtga ctttaccaag actggtatcg
    11941 gtgttgccca agcggttgct atccctcttg ccgtagctgg acttttcttg acaatgattg
    12001 ttcgtacaat ttcagttggt ttggttcata ctgcagatgc tgccgctaaa aaaggtgact
    12061 tcggcgctgt ggagcgtgcg catttcattg cgctactttt ccaaggactt cgtatcgcgc
    12121 ttcctgcagc tcttctcctt atggtaccaa ctgaaactgt acaaagtatc cttagtgcca
    12181 tgccagactg gctcaaagat ggtatggcta tcggtggtgg tatggtcgtt gccgttggtt
    12241 acgccatggt tatcaacatg atggcaactc gtgaagtatg gccattcttc gctcttggtt
    12301 tcgttctcgc tgctgtgtca gatattactc taatcggatt cggtgctatc ggtgttgcta
    12361 tcgctcttat ctaccttcac ctttctaaaa ctggtggaaa tggtggcgga ggagccgcaa
    12421 cttctaacga cccaatcggc gatatcctag aagactacta agataagaaa ggactgaaaa
    12481 catcatgact gaaaaacttc aattaactaa atcagatcgt aaaaaagttt ggtggcgttc
    12541 aaccttctta caagggtctt ggaactttga acggatgcaa aacttgggct gggcttatac
    12601 actcattcca gctatcaaaa aactctatac taaaaaagaa gatcaaatcg ctgctcttga
    12661 gcgtcacctt gagttcttca acactcatcc atacgtagct gctccagtca tgggggttac
    12721 tcttgcgctt gaagaagaac gtgctaacgg tgtggaaatc gatgacgctg ctatccaagg
    12781 ggttaaaatc ggtatgatgg gacctcttgc tggtatcggt gacccagtat tctggtttac
    12841 agtgcgccca atccttggat ctctcggtgc ttcacttgcc cttactggca atatcttggg
    12901 tccactcctc ttctttgttg catggaactt gattcgtatg tcattcttgt ggtatgttca
    12961 agagattgga tacaaggctg gatcagaaat cactaaagat atgtctggtg gtatccttca
    13021 agatatcact aaaggagctt ctatccttgg gatgttcatt cttgctgtcc ttgttcaacg
    13081 ctgggtaaat attaaatttg ctttcgatgt ttctaaagtt caactagatg aaaaggctta
    13141 tatccattgg gataaattgc cagaagggtc taaaggtatc caagaagcat tcgcacaagt
    13201 aggacaagga ttgtctcaaa ctcctgaaaa agttactact ttccaacaaa acttggatat
    13261 gttgattcct ggattatcag gactactcct tactttactt tgcatgtact tacttaagaa
    13321 aaaagtatct ccaatcacta ttatccttgc cctcttcgca gtgggtattg tggcacatgt
    13381 tcttcacatc atgtaatcaa gcaactaaaa aggaaccagg ttctaaaatc tgattccttt
    13441 tttctatgct tttattcagc caaggctccc attggatccc atggtgcaag tacgattggt
    13501 tctgctccat aggcagcttg ttcttctgct gtcagcaatt ccttacgaac aacgatttgg
    13561 tatgtgtatt cgtccatcca agcgtctgag gcaacaaagt aaccatctgt accgaccttg
    13621 tctccccatg agttttcaac cttccacttg gttgatttac cattttcgtc caagtcaaca
    13681 cctgtcaaga ccatggcgtg ggtcatcaag ctttcgctat agtccaaacg tccagccttg
    13741 tcttgagtaa gtttaatgtc catgcttgat tcaaagtcat aaacatctgt cgcaaggatg
    13801 ccagccttac ggttgctgag ctggccgaca tcagaaccaa accaaacagt ctcacctgct
    13861 tgcatttggg caatcgccaa ttctttcaag cgctccattg gaacgttgat gtagcgaact
    13921 gcacggctac caaccacatt ccccaacatc tcaactgtgt aagattttcc gtaaggttta
    13981 tcagcagttg gagcattgat aacagaaacg tagtcttcta aaggaagatt gacatatttc
    14041 ttgtaaaact cttgtggtgt gattcctttt tcacttttgt agttgttatc tttatcgcga
    14101 taagcaaagt caaacttgcg tggtggaagt cctaatgaca tagcaagaaa gttaaagatt
    14161 tcttgcaaga ggtcttcttt cttagcttga acagtcgctt gatctgcacc agaaacaagc
    14221 aagtcacgca agatttgagc atcttgacga agcaatttat taaggatcgc atttagctca
    14281 cgactgctgc tagatgaaac agactcagga taaactgact taggcacgac accgtatttt
    14341 tcaaagaggg aaacgaccat atcccattga ccaccatctt gttgaggtgt ttggagtaag
    14401 aagctaacct tgcggctagt caattcttgg tctgaagtcg caatgacttg ctccaagaac
    14461 cagtttgatt tctcatactt atcccagaag aaagtgtggg cttgtgacaa ttcaaagttc
    14521 tccaatttgt attgcgagat gagtttgtgg cggaaggtgt tgagagccgc aaacatccag
    14581 caacgaccag acgctttctg gttagtgacc ttgtccttgg ttaaatccaa tgagaaaaca
    14641 ggtgtgttgt ctacatggct ttggcgacgt tccagagctg caaaaattcc gttgtggctg
    14701 gcagcatttt caatcgcttg gtattttaca tttgcttcat agttggcaaa tagtttatca
    14761 gtaaatgatt cttgaatcgc gttcatagat tcctcctttt attctacagt ctattataac
    14821 tcttttcaca aaggaagcaa ctaaaaaaca taaaaggcaa ggacattctc cctcaccttt
    14881 ttcgaattta tactcttcga aaatcaaatt caaaccacgt caacgtcgcc ttgccgtact
    14941 caagtacagc ctgcggctag tttcctagtt tgctctttga ttttcattga gtattaaact
    15001 aaattaaata atattagcaa ggagaatttc taattcttcc ttggtcaagc gacgccattc
    15061 ccctcgttcc aggttctcat ctaatactaa agttcccata gtcaatcgtt gcaagtccac
    15121 cacttccttg ccacagtagc ccaccatacg cttgatctga tgaaacttcc cttctgcaat
    15181 ggtcacacgg atttggcttt gattcttttc tgtatctatg gatacaagct ccagtatagc
    15241 gggttgacag gtaaagtctt tgagaggaat accctcagca aatgtctcca catcttcttg
    15301 ggtcatgatt cccttgactt gtgccagata agtcttgtcc acatgacgct tgggcgaaag
    15361 aagaacatga gccagctgac catcattggt caagagcaaa agaccatgcg tgtcaatatc
    15421 caagcgtcct actgggaaaa cttccttact ccgcgccaag tcatccaaca agtccagaac
    15481 ggttctgtgc ttgggatcct cagtcgctga gataactcct ttgggcttgt tcatcatgta
    15541 gtagacaaac tcttcatact ccaacacttg cccatcaaag cgaatctcat ctattttttc
    15601 atcaatctgc aatttagctg atttttcttt tttaccattt acagtcacgc gcccagcctt
    15661 gagcaagttt ttgacctcag tccgacttcc caccgcacag gcaactaaaa atttatctaa
    15721 tctcatagaa ctattatatc atatcaaaag gaggctagta caatgaccaa cctccttttc
    15781 gtttcatact cttcaaaaat ctcttcaaac cgcgtcaacg tcgccttgcc gtat
//
LOCUS       DAVBDG010000044        15729 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBDG010000044 DAVBDG010000000
VERSION     DAVBDG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.045"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(1..228)
                     /locus_tag="VQ526_001464"
                     /pseudo
     CDS             complement(1..228)
                     /locus_tag="VQ526_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            353..811
                     /locus_tag="VQ526_001465"
     CDS             353..811
                     /locus_tag="VQ526_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2555176.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            824..1432
                     /locus_tag="VQ526_001466"
     CDS             824..1432
                     /locus_tag="VQ526_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555177.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1503..1835
                     /locus_tag="VQ526_001467"
     CDS             1503..1835
                     /locus_tag="VQ526_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555178.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1846..3261
                     /gene="ply"
                     /locus_tag="VQ526_001468"
     CDS             1846..3261
                     /gene="ply"
                     /locus_tag="VQ526_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2555179.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            4142..4858
                     /locus_tag="VQ526_001469"
     CDS             4142..4858
                     /locus_tag="VQ526_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2555180.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            4982..5451
                     /locus_tag="VQ526_001470"
                     /pseudo
     CDS             4982..5451
                     /locus_tag="VQ526_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5468..6289
                     /locus_tag="VQ526_001471"
                     /pseudo
     CDS             5468..6289
                     /locus_tag="VQ526_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            6296..7267
                     /locus_tag="VQ526_001472"
     CDS             6296..7267
                     /locus_tag="VQ526_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2555181.1"
                     /translation="MDFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPAQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSIGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQRVAIVGPTGAGKTTIVNLLMKFYEIDKGSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMV"
     gene            7393..7755
                     /locus_tag="VQ526_001473"
     CDS             7393..7755
                     /locus_tag="VQ526_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HET2555182.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLTCLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            7752..8255
                     /locus_tag="VQ526_001474"
     CDS             7752..8255
                     /locus_tag="VQ526_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2555183.1"
                     /translation="MKNYQEWYRNIRSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            8369..8815
                     /locus_tag="VQ526_001475"
     CDS             8369..8815
                     /locus_tag="VQ526_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555184.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKSPRLIF
                     YKGTGEYYLDLSEILFFETEGSKIYAHTQKNAYEVRLKLYELEAILPRYFSRVSKSTI
                     VNIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            8820..9494
                     /locus_tag="VQ526_001476"
     CDS             8820..9494
                     /locus_tag="VQ526_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555185.1"
                     /translation="MKKKAFGIVLLVLAALILVQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVALMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYDGEKNILIDKEVAFGTGTFYKQDQELVEDQVEVGFGNAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYAK"
     gene            9655..9846
                     /locus_tag="VQ526_001477"
     CDS             9655..9846
                     /locus_tag="VQ526_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2555186.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            10131..10430
                     /locus_tag="VQ526_001478"
     CDS             10131..10430
                     /locus_tag="VQ526_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2555187.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            10427..10744
                     /locus_tag="VQ526_001479"
     CDS             10427..10744
                     /locus_tag="VQ526_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2555188.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            10760..11386
                     /locus_tag="VQ526_001480"
     CDS             10760..11386
                     /locus_tag="VQ526_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2555189.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            11419..12180
                     /locus_tag="VQ526_001481"
     CDS             11419..12180
                     /locus_tag="VQ526_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2555190.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            12258..12653
                     /locus_tag="VQ526_001482"
     CDS             12258..12653
                     /locus_tag="VQ526_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2555191.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            12923..13207
                     /gene="groES"
                     /locus_tag="VQ526_001483"
     CDS             12923..13207
                     /gene="groES"
                     /locus_tag="VQ526_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2555192.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            13223..14845
                     /gene="groL"
                     /locus_tag="VQ526_001484"
     CDS             13223..14845
                     /gene="groL"
                     /locus_tag="VQ526_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2555193.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEVTGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            14983..15729
                     /locus_tag="VQ526_001485"
                     /pseudo
     CDS             14983..15729
                     /locus_tag="VQ526_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567576.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         4689 a         2868 c         3616 g         4556 t
ORIGIN      
        1 tcaactgtta gtggcttgag tttgctggat ttacgtggag tttgtgcttg aggatatatc
       61 ttcatgagcc cttgataatt actgtcagcc aagattttac cagcttgtcc gacttttttg
      121 ataagctgtt tttaatacag ctaacatctc tttaaaagtg gtacgctgaa cactaacaag
      181 acgcttaaat cgtgcatcag ttagttgttt acttgcttca taattcatag aactactata
      241 ccatattttg tttcgcagga agtctaataa gttatagtcc agtttttact atactttgat
      301 attcaaatat gttatactat ggttatcaaa gtaaatgaaa ggagttacga atatgacgcc
      361 agaagaaatg tacctgacag agcgattaga cgtacagata gctcattttt taaagaaaag
      421 cgttcaacat cgtaggcgct ataaggtatt aaaaataaca gaaatcgtgg caggtttcct
      481 catagctgtc ttttgtgcta ttcctatgcc aggtgatcgc taccgtttga tttcggttgc
      541 cttatccagt ctcggcttgc tgtgtgaggg gattatcaat ttgtataatg caaaggaaaa
      601 ttggatttct taccaaaaaa ctgcgcaact cctggagaaa gaaaaattcc tctatcaatg
      661 ccaaacggag aaatatgcag gaaagaccaa ggcttttgcc ctatttgtca agacatgcga
      721 aggtcttatc tcagaggaga ttaaccagtg ggaaagtatc cagtcaaaag aagtggcagc
      781 tagtgcagat gctccagtta aaaaagagta ggaggtagag gaaatgtctc aatccagtta
      841 cctgtcgccc ttgctctggt taaaaaaaga agccgataag gaaaagatga gcgcgaccca
      901 gtgccagata tttttctttt actatcaaat gtttgagctc ttatttgcta gagaaagcga
      961 catgaaagac ttatgtctgg gaacgaaagg tttttatttc tcgcagttag agaaaaattt
     1021 gctttctgga gtttcccgat ttctaaaaaa cttggagggg aaagtaactc tcaaggctaa
     1081 ccaagaagta tcagctcgca aagccctttt tctagccttg acaactagcc aatcagattg
     1141 gcaggagtta gctcctgttt ttgattttta tcagactatc gggaggcttg aaaatccttc
     1201 tctcttgagt tctcaggaca gacaacatct gatgtggatt taccagtcag ctttggagaa
     1261 ggattatatt gtcaaggtta ttggcgacaa gcattttgta ttgaagagac aagatgctac
     1321 taaattgaca gcgcgccaaa ctcaaacttt ggaaattctg agtcaatcag aagacttggt
     1381 caatcctgtc tatgttacat taggagaaaa gggggtgctc ttgcttgatt aagagaggag
     1441 atgttgtagc tctttatttg ccttttccga ctattagtag cgatttggct gtgaagaatc
     1501 atatatatat ctgtattgac aacagcatga ctaaaaacaa agagttggtt aaaaatcaga
     1561 ccttcaaacc agctctcttg accagacgtt tggtcaagaa ctttatgata gaagagccgg
     1621 atctagctcg taatcctttt acaagaccaa ccttgattga cttagataag gtatttatgt
     1681 tggataatac ggttattccg acttcttatc tagccagacg gcgacgcaat gtctcagaag
     1741 aattgtacga ggaaattttg gatcacttag tccaaccacg gctgatttcg ctgaacaagt
     1801 ctgagtttat gcaactcaat ccaggaactt attaggaggt agaagatggc aaataaagca
     1861 gtaaatgact ttatactagc tatgaattac gataaaaaga aactcttgac ccatcaggga
     1921 gaaagtattg aaaatcgttt catcaaagag ggtaatcagc tacccgatga gtttgttgtt
     1981 atcgaaagaa agaagcggag cttgtcgaca aatacaagtg atatttctgt aacagctacc
     2041 aacgacagtc gcctctatcc tggagcactt ctcgtagtgg atgagacctt gttagagaat
     2101 aatcccactc ttcttgcggt cgatcgtgct ccgatgactt atagtattga tttgcctggt
     2161 ttggcaagta gcgatagctt tctccaagtg gaagacccca gcaattcaag tgttcgcgga
     2221 gcggtaaacg atttgttggc taagtggcat caagattatg gtcaggtcaa taatgtccca
     2281 gctagaatgc agtatgaaaa aatcacggct cacagcatgg aacaactcaa ggtcaagttt
     2341 ggttctgact ttgaaaagac agggaattct cttgatattg attttaactc tgtccattca
     2401 ggcgaaaagc agattcagat tgttaatttt aagcagattt attatacagt cagcgtagat
     2461 gctgttaaaa atccaggaga tgtgtttcaa gatactgtaa cggtagagga tttaaaacag
     2521 agaggaattt ctgcagagcg tcctttggtc tatatttcga gtgttgctta tgggcgccaa
     2581 gtctatctca agttggaaac cacgagtaag agtgatgaag tagaggctgc ttttgaagct
     2641 ttgataaaag gagtcaaggt agctcctcag acagagtgga agcagatttt ggacaataca
     2701 gaagtgaagg cggttatttt agggggcgac ccaagttcgg gtgcccgagt tgtaacaggc
     2761 aaggtggata tggtagagga cttgattcaa gaaggcagtc gctttacagc agatcatcca
     2821 ggcttgccga tttcctatac aacttctttt ttacgtgaca atgtagttgc gacctttcaa
     2881 aatagtacag actatgttga gactaaggtt acagcttaca gaaacggaga tttactgctg
     2941 gatcatagtg gtgcctatgt tgcccaatat tatattactt gggatgaatt atcctatgat
     3001 catcaaggta aggaagtctt gactcctaag gcttgggaca gaaatgggca ggatttgacg
     3061 gctcacttta ccactagtat tcctttaaaa gggaatgttc gtaatctctc tgtcaaaatt
     3121 agagagtgta ccgggcttgc ctgggaatgg tggcgtacgg tttatgaaaa aaccgatttg
     3181 ccactagtgc gtaagcggac gatttctatt tggggaacaa ctctctatcc tcaggtagaa
     3241 gataaggtag aaaatgacta gtagaggaga atgcttgcga caaaaagagg cgatgatctc
     3301 tctgcggata tctgaaagtt tatctcttgc ctagcgattt ccattggagt atgctcgtga
     3361 tgttaaaatg aatctatcaa acagaatgtg taaaaaagta aaaagggtag ccaattcctt
     3421 gatatacagg ggattgatta tcctttactt gttttagatg attggaaatt caagaatgac
     3481 acgtatcgga aatattttgc tgtttatatc taatacccat ttatggaaaa aatgaaatta
     3541 aatatgaaca aatcaattgc tagcaatggt ttagaagtgc cagtgtacta gtctaatttc
     3601 tccatcttgg gaacacgtaa attatactct tcgaaaatct cttcaaacca ggtcagccct
     3661 atccgcaacc tcaaaacagt gttttgagca acctgcggct agctttctag tttgcttttt
     3721 gatttttatt gagtattaga ggtttgctta gaaactcttc tattgatttc ttagaataca
     3781 atagcgccta taagaactgt tggatttcaa agtatttact tgaactatga tatgtctgga
     3841 gtagactgtc aacaaaaggt gcagtatgat tgtttttgtc gcttatatct acaaacttca
     3901 aacagtaatt tgagctgact ttgtcaaact tggctgaaac tttctatttg cagataatgg
     3961 tctagtttgt tttttgaatg tggatgagtg tctgttgata aagatagaaa ggtaagacca
     4021 tcttagcagg taaagtgcta tcctagcttg atttgctaat aaaatccatt tcctctagtg
     4081 aaaatcaaaa aaacttgtgt tataataaga aagattaaaa tgtgaaaaaa ggagattcct
     4141 aatgggacgt aaatgggcca atatcgtagc caagaaaacg gctaaagatg gagctaactc
     4201 taaagtatat gcaaaatttg gtgtagaaat ctatgtagca gctaaaaaag gtgatccaga
     4261 tccagaatca aactcagctt tgaaattcgt tatcgaccgt gctaaacaag cccaagtgcc
     4321 aaaacacatt atcgataaag cgattgataa agccaaagga aacacagacg aaacctttac
     4381 agaaggacgt tacgaaggtt ttgggccaaa tggctctatg ctaattgtgg atactttgac
     4441 ttctaacgtt aaccgtacag cagccaatgt ccgtgcagcc tttggtaaaa acggcggaaa
     4501 catgggcgct tcaggttctg tttcttacct ttttgacaac aaaggtgtta ttgtatttgg
     4561 aggtgaagat gcggacgcag tctttgagca attgctcgaa gcggatgtgg atgtggatga
     4621 cgtagaagca caagaaggta caatcacagt ttacacagcg ccaactgacc ttcacaaggc
     4681 tatcgttgct ctccgtgagt ctggtattga agaattccaa gtgactgaat tggaaatgat
     4741 tcctcagtca gaagtggaat tgtcaggcga agaccttgaa acctttgaaa aactttacag
     4801 cgttcttgaa gacgacgaag acgtccaaaa gatttatacg aacgtagatg ggttttaatt
     4861 taacctatag aacatgatcc taagtgagaa aatcacttgg gattttttta atcaagaaaa
     4921 tagttctcat agaaatttaa ttattctcca gagaactatt tttatttaga agggggagat
     4981 tatggataaa ccgatgttag tctttaagcg ttttggtcat caaatttacc tgatggtgca
     5041 aaaggaagcc aaacgttgcg gcattgaatt tatgggtggg ccgcaaggtc aggttgtgcg
     5101 ttttttggat aatcgcgaga aaaaccaaga cttggtcttg attaaagata tcgagcagga
     5161 actcaatatt accaagtctg ttgctagtaa tctggttaag cgtatagtgc aaaatggttt
     5221 ggtggaattg gaggcgagtc ctgttgataa gcgggctaag tttgttcgtc tgacggacaa
     5281 agcacgttct cagatgcaac aggttaaggc ttttttgaac gcatagacaa gcagttgatg
     5341 gaagacattg atgaagatga attactgatt tttgagaaag ttctcggtca actacaggca
     5401 aatatcaagg gaataggagg agagaataaa gaaattagcc aaaagaatta gtagaaaaga
     5461 atgggggatg attttactag ccattctctt tacttgcttt tcggtctatc tagagttgga
     5521 agtgccgacc tatatctcga aaattacgga tttgctaggt agtcaaggaa ctaatttaga
     5581 tgagttgtgg cagccggcaa gcatgatgat gggaatgccc tttcttgcct tcttgtccgt
     5641 agttgcagtt ggattttttg catcccgagt ggcggcttct tatactagta ggctgagaag
     5701 tgatattttt aaccgagttt tggattactc gcagacagag attaagaaat tttcaattcc
     5761 tagcctcttg atgcgtacta ccaatgacat tactcaagtt caaatgttga ttactatgga
     5821 cttgcaagtg gtaacgtgtg gtccaattat ggctatctgg gctattggga agattttagg
     5881 tcattcagaa tactggctct gggccgtact tgtggcagtg attgtcaacg tcctgatgac
     5941 gaccgttttg atgacgctag cctttccaaa acagtccttg attcaggggc tgacagataa
     6001 actgaacagt atcactcgtg agagtttaac aggtattcgt gtcgttcgtg cctacaatgc
     6061 agaggattat caaaatgaaa aatttgcagc agtaaatgat gaattgaccc gttaaaattt
     6121 gtttgtcaac cgtcttatgg ctattttgaa tcctatcatg atggggattt caagtggttt
     6181 gagtgtggcg atttactgga ttggggccta tgtgattaac gacgctgctc cgatagcgcg
     6241 tctgcctttc tttagtgaca tgattgtttt catgtcttat gccatgtagg ttgtcatgga
     6301 cttccttctc atgggagcac tcttcatcgt tcttccccga actatggtct ctgctaagcg
     6361 gattaatcaa gttttagatt tgcattcttc tatccaaaac cctgctcaag tgcagctgac
     6421 tgatgaaaac ttcaaaggtc aggtcgagtt taaggatgtg accttccgct atgcggcaaa
     6481 ttcggaggca gttattgaac atgttagctt taaagcagaa actggtcaaa cagtggcctt
     6541 tattgggtca ataggttctg gtaaatcaac tctggtcaat ctgattccac gtttctacga
     6601 cgtgtcagca ggagaaattc tggtggacgg tgtcaatgtt caagactatg acttctctgc
     6661 gacagctcat gctggtcaaa gggttgccat tgttgggccg actggggctg gtaagacaac
     6721 cattgtcaat cttttgatga aattctatga gattgataag ggaagtattc gcattgatgg
     6781 tgtggatacc aaggctatga cgcgttcaga agtgcatgat gccttttcaa tggtcttgca
     6841 ggatacctgg ctctttgaag gaactattcg agacaatctc atctataatc aaatagggat
     6901 tagtgatgaa cgaatgatgg aagctagtaa ggctgtggga attcaccact ttattatgac
     6961 cttgccagat ggctatgata ccatcttgga tgacaccgtg accttgtctg taggacaaaa
     7021 acaactattg actattgctc gtgcccttct taaggatgca ccgcttttga ttttggatga
     7081 ggcgacttct tctgttgaca cacggacaga ggaattgatc caaaaagcca tggaccgttt
     7141 gatggaagga cgcacatcct ttgtcattgc ccaccgcttg tcaaccatcc gaaatgcaga
     7201 cttgatcttg gtcatgaaag atggaaatat catcgagcaa ggcaactatg aggaactgat
     7261 ggtgtaaggt ggcttctacg ctgacttgta caatagtcaa tttacagaag atgaagcaga
     7321 agaataaatc aaaagaaggc ttgacggcct tctttttctt atttatacta gtacacatag
     7381 aaagagagaa gtatgagtca gaaattatac aatatgaaat ttgctgctgt ctacttagcc
     7441 ttgattgcaa aagttgagcg aaagggtgga aaggcagagt cagttcacca agtgaccagt
     7501 tggttgacag ggtatgaagt gagtgacgtt cttacttgtc tggataggga tgttacttac
     7561 ggagattttt ttcgacaagc accatattat gtccctgaac gaattgcaat tacagggaag
     7621 atttgcggtg tccgtattga ggaaattgat gatcctctga tgcaggaaat acgtcgcttg
     7681 gacaagctag tggattggct tgccaagggg aaaacatctc aacaagttct agaaaagtat
     7741 gagaagcata aatgaaaaat tatcaagaat ggtatcgaaa tatcagatcc agactaacta
     7801 gtcgtccaat tcttttattc ctattacgca gtttcaatcg tttgatgaca gtcgccatgc
     7861 ccctggtcta tctgatcttg ctagtcacca cttacctgca actaggactt ggtcagcaag
     7921 ttggagttta tgtgcttatt cctgcatcag gttttgtggc cttgtccctg tttcgtaaaa
     7981 aaatcaatca cccgagacct tatgaaactt gggatatctg tccccttctt gataaggata
     8041 gttcgggacg gtcgatgccc agtcgccatg tcttttctgc aactatcatc tccatggcct
     8101 gtctgcatgc tagtctacct attggcttag tatgcttgat cttttcagct ttgctggggt
     8161 tggtgagggt tttagggggt gttcattatc ccaaggatgt cttggttggc tatgcttgtg
     8221 gtctggtgtg gggattcctt ttcttcttat tctgacatgt aagttaagct agtcctacaa
     8281 ccacttactg cttcaaattg accacttgct tttttgccct tgtattccta acttagcttt
     8341 gatatagtag agtcagaaag gagatgtgat gaagttacga attgagattg acggcaattt
     8401 agaggagact gaaattgtca tcaagacccc cactttgaca gatgaaattg cagacttgca
     8461 acggctcttg caagaatcca agtctcctag gttgattttt tacaagggga cgggtgaata
     8521 ttatctggac ttgtcggaaa ttctcttctt tgagacagaa ggtagcaaga tttatgccca
     8581 tacccagaaa aatgcctacg aagttcggct caaactctat gagttagagg ctatccttcc
     8641 tcgctatttt agtcgagtat ccaagtcaac gatcgttaat attcggcaaa tctattcggt
     8701 ggacaagtcc ttttcaggaa cgggcaccat ttccttttat caaacccaca aggaggttca
     8761 tgtgtcacgg cattaccaat ccctcctaaa agaaaatcta agaaatatga ggtaagaaca
     8821 tgaaaaagaa agcatttggt attgtgttgc ttgtactagc agctttaata ttagtacaag
     8881 gaaattttgg aatcccttct ctaggagggc acatttggcc cttgattggt attgcttttt
     8941 ttgcctacca atcagttgga gctttgttgc gtcgccactt aacttcagcc tcttttacag
     9001 ctctagtagc cttaatgatt gctaaccact tttatggcat tttaccgatt ccaaaccaga
     9061 cactaatctg ggcaggagtt ctaatcgttc tcggagtaaa tatgttgact cattctaaca
     9121 gaacttggaa tgggaaaaaa tggtggtatg atggtgaaaa aaacattctt atagataagg
     9181 aagttgcttt tgggacaggg actttctaca agcaagacca agaattggta gaagaccaag
     9241 tagaagttgg ttttggcaat gcaaaaatct actatgacaa tgcagagatg ttaggtgatt
     9301 ttgcaacttt aaatattgaa gtagcttttg gtaatgcaac tgtctatgtt ccgcaacact
     9361 ggcgtgtcga tttgaaagta gaaacatcct ttggtgcagc caaggcggac gctcctgtag
     9421 ccccaaccag caaaaccttg attatccgtg gagatgtggc ttttgggaag ttggaaattg
     9481 tctacgctaa ataaaaaaat cttcacttca accatcaaaa taggcgtact aagagtagga
     9541 aattgatgcc ttgctctgat ttcagttcta tggttgttag actttaaaaa atgaaatgct
     9601 gcctttaaaa gttgtatatt tttcgatatt ttggctttta cgtttgatgt atctatgtac
     9661 tacagcgtag atgatgtagt gtcgaatgct tttaaaaaac gaatgatatt ggacagtttt
     9721 tttgccttta attgctcagg aaccatgaaa gtcagtactt gggtttatga caagggagaa
     9781 tggtattatg taagttcttc tggttccatg attgcgaatg attgggtcaa agacaatggc
     9841 aagtagtatt atctggcttc atcaggtaag atgcttcgca atacctacac acctgatggt
     9901 tactacgttg gcaacttagg tgcctggcaa taagaataat actctttgaa aatctcttca
     9961 aaccacgtca acgtcgcctt atctacaacc tcaaaaccgt gttttgagca gcctgcggct
    10021 agctttctag tttgctctta gtataagaag tccttttcct taaaggaaga ggctttttac
    10081 ttgcttttct gctgcttcct catttgtcct gaagcctttt ttgtgttaaa atgaatggta
    10141 tgaaagctaa aaaaatgtgg atggcaggct tggctctgct aggtatcgga agccttgctc
    10201 ttgctacgaa aaaagttgca gatgaccgta agctcatgaa gactcaggaa gagttgacag
    10261 agattgtgcg agaccatttt tccgacatgg gggaaattgc gaccctttat gttcaagttt
    10321 acgaaagcag tctggagagc ttggttggtg gcgtcatttt tgaggatggc cgtcattata
    10381 cctttgtcta tgaaaatgaa gacctagtct atgaggagga agtcttatga tacagccagc
    10441 aagtttagaa gaattagcat ctttagtgga aaaagcgggc aagaaggtct tcctttttgt
    10501 ggcagactgg tgtggcgatt gtcgttatat ttatcctgcc ttaccagaga ttgaagagac
    10561 caatccagag ttcaccttta ttcgaatgga ccgagatcag tatatggatt tggccaaact
    10621 ctgggatgtt tacggaattc ctagccttgt tgttctagaa aaggacaagg aaatcggtcg
    10681 ttttgtcaat cgcgaccgta aaagtaagga gcaaattaac gattttttag caggattgaa
    10741 ataggagaaa aaggaaacaa tgatttttac gtataacaaa gaacatgtca gtgatgtcct
    10801 tatggtcatc gtgaaaaata gcggagatgc caaactgaat gtggaacgca aaggcaaggt
    10861 agcccgtgtt ttcctcaaag aaaatgggga aacagtagct tggaacattt ttgaggtttc
    10921 gagcttgttt gaaattgcag agcgcggtca agtcttttta tcagatgagc aagtcgctcg
    10981 tttgaaccaa gaattacagg cggaaggttt tacagaagaa attgttaatg ataaggaacc
    11041 taagtttgtt gttggtgaaa ttgtcgagat ggtagctcat ccagacagtg accacctcaa
    11101 tatctgccaa gttgcagtcg caagtgacaa gatagtgcaa atcgttgcag gagcacctaa
    11161 tgcgcgtgtt gggttgaaaa ccattgtggc tcttcctggt gccatgatgc caaaagggaa
    11221 tttcattttc ccaggcgaac ttcgtggtga aaagagtttt gggatgatgt gtagtcctcg
    11281 tgaattgcat ttgccaaatg ctccgcaaaa acgtggggtg cttgaattat cagaagacca
    11341 agttgtcgga acaccgttcg acccagcgaa acactggact gcctaggaac ttgtcagcat
    11401 ctgatagaag gaaataagat ggcaaaaaat gtagtgatta cgggagcaac ctcaggaatc
    11461 ggtgaagcga ttgcgcgtgc ttatctggag cagggtgagg atgtcgttct aacaggacga
    11521 cggatagaca gattagaaat cctcaagtcg gagtttgcag taagctttcc aaatcaaacc
    11581 gtctggactt ttccactaga tgtgacggat atggtcatgg tgaagactgt ttgctctgat
    11641 attctagaaa cgatagggag gattgatatc ttggtcaaca acgccggact ggctcttgac
    11701 ttggctccct atcaagacta tgaggagttg gatatgttga ccatgttgga taccaatgtt
    11761 aaaggtctga tggcggttac tcgctgtttc ttgccagcaa tgataaaagt caatcaaggt
    11821 catattatca atatggggtc aaccgcagga atctacgcct atgctggtgc cgctgtttac
    11881 tcagctacca aggctgcggt taagaccttt tcggatggac tgcgaattga taccatcgca
    11941 acggatatca aggtgacaac cattcagcct gggattgtcg aaacagattt ctcaactgtt
    12001 cgttttcatg gtgataaaga gcgagctgcg tccgtttacc aaggaataga agccttgcaa
    12061 gctcaggata ttgcagacac agtagtctat gtgaccagtc agcctcgccg tgttcagatt
    12121 acagatatga ccattatggc caatcaacag gcgacaggtt tcatgattca taaaaaataa
    12181 gaaatttcct cgaaaagtta caaatttctg taactttttt tgatttccta cgaatagata
    12241 agtaggagga agaaaatatg tataataaag ttatcatgat tgggcgttta acgtctacac
    12301 cagaattgca caaaaccaac aatgacaagt cggtagcgcg agcaactatc gctgtgaacc
    12361 gtcgttacaa agaccaaaac ggtgaacgtg aagctgattt tgtcaatatg gtcctatggg
    12421 gcagactagc agaaactttg gcaagctacg caaccaaagg tagtctcatt tccgttgatg
    12481 gagaattgcg tacccgtcgc tttgagaaaa atggccaaat gaactacgtg accgaagtac
    12541 ttgtaacagg attccaactc ttggaaagtc gtgctcaacg tgccatgcgt gaaaataatg
    12601 caggccaaga tttggcagat ttagtcttgg aagaggaaga attgccattt taatactctt
    12661 cgaaaatctc ttcaaaccac gttagcttta tccacaacat caaagcaatg ctttgagcag
    12721 cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaaca ttaaaaagtc
    12781 tgagttggtc tcaggctttt tatcttgaga aagtcagact tttttcttga ttatttctga
    12841 ctaagtgata caatagaact atgaattagc actcaggtat aaagagtgct aataatatct
    12901 atctcattat ggaggaaatc agatgttgaa accattaggg gaccgtgtgg tcttaaaaat
    12961 agaagaaaaa gaacaaaccg ttgggggctt tgttctcgca ggttcagccc aagaaaaaac
    13021 caaaacagcc caagttgtgg ctactggaca aggtgttcgt accttgaacg gtgacttggt
    13081 tgctccaagt gttaaaactg gagaccgtgt cttagttgaa gcccacgcag gtcttgatgt
    13141 caaagatggc gatgaaaagt acatcatcgt aggcgaagct aacatcttgg caatcattga
    13201 agaatagaag gagaaagtaa gtatgtcaaa agaaattaaa ttttcatcag atgcccgttc
    13261 agctatggtc cgtggtgtcg atatccttgc agacactgtt aaagtaacct tgggaccaaa
    13321 aggtcgcaat gtcgttcttg aaaaatcatt tggctcacca ttgattacca atgacggtgt
    13381 gactattgcc aaagaaatcg aattggaaga ccattttgaa aatatgggtg ctaagttagt
    13441 atcagaagta gcttctaaaa ccaatgatat cgcaggtgac ggaactacga ctgcaacagt
    13501 cttgacccaa gctatcgtcc gtgaaggaat caaaaacgtc acagcaggtg caaatccaat
    13561 cggtattcgt cgtgggattg aaacagcagt tgccgcagca gttgaagctt tgaaaaacaa
    13621 cgccatccct gttgccaata aagaagctat cgctcaagtt gcagccgtat cttctcgttc
    13681 tgaaaaagtt ggtgagtaca tctctgaagc aatggaaaaa gttggcaaag acggtgtcat
    13741 caccatcgaa gagtcacgtg gtatggaaac agagcttgaa gtcgtagaag gaatgcagtt
    13801 tgaccgtggt tacctttcac agtacatggt gacagatagc gaaaaaatgg tggctgacct
    13861 tgaaaatccg tacattttga ttacagacaa gaaaatttcc aatatccaag aaatcttgcc
    13921 acttttggaa agcattctcc aaagcaatcg tccactcttg attattgcgg atgatgtgga
    13981 tggtgaggct cttccaactc ttgttttgaa caagattcgt ggaaccttca acgtagtagc
    14041 agtcaaggca cctggttttg gtgaccgtcg caaagccatg cttgaagata tcgccatctt
    14101 aacaggcgga acagttatca cagaagacct tggtcttgag ttgaaagatg cgacaattga
    14161 agctcttggt caagcagcga gagtgaccgt ggacaaagat agcacggtta ttgtagaagg
    14221 tgcaggaaat cctgaagcga tttctcaccg tgttgcggtt atcaagtctc aaatcgaaac
    14281 tacaacttct gaatttgacc gtgaaaaatt gcaagaacgc ttggccaaat tgtcaggtgg
    14341 tgtagcggtt attaaggtcg gagccgcaac tgaaactgag ttgaaagaaa tgaaactccg
    14401 cattgaagat gccctcaacg ctactcgtgc agctgttgaa gaaggtattg ttgcaggtgg
    14461 tggaacagct cttgccaatg tgattccagc tgttgctacc ttggaattga caggagatga
    14521 agtaacagga cgtaatattg ttctccgtgc tttggaagaa cctgttcgtc aaattgctca
    14581 caatgcagga tttgaaggat ctatcgttat cgatcgtttg aaaaatgctg agcttggtat
    14641 aggattcaac gcagcaactg gcgagtgggt taacatgatt gatcaaggta tcattgatcc
    14701 agttaaagtg agtcgttcag ccctacaaaa tgcagcatct gtagccagct tgattttgac
    14761 aacagaagca gtcgtagcca ataaaccaga accagtagcc ccagctccag caatggatcc
    14821 aagcatgatg ggcgggatga tgtaagcatt ctatagaaaa caacttataa aaaacacaaa
    14881 aggagggaat gactaaccct tctttttata atattcactt ctaaattgat ttgagctctc
    14941 ctaacttata tgataaaata agactagaaa aaggagaaga acatgatcga tgtagaaaaa
    15001 attctgtgca agatgccccc caatcagaag attaattatg accgtgtcat gcagaaaatg
    15061 gtagaagtat ggtagaaaaa tgagcaacgg ccaaccattc tcgtgcatgt ttgctgtgcc
    15121 ccttgtagta cctatacact agaatatttg accaagtatg cagatgtgac catctatttt
    15181 gccaattcta atatccatcc caaggtagaa taccataagc gggtctatgt caccaagaaa
    15241 tttgttagtg attttaatga gcggacagga aatacggttc agtacctaga agctccctac
    15301 gaacccaatt aataccgaaa actagttagg ggactagagg aggagcccga aggtggcgac
    15361 cgttgcaagg tttgttttga ctaccgactg gataaaacag cgcaagtggc tatggacttg
    15421 ggctttgact actttggttc agccttgacc atcagtcctc ataagaattc tcaaaccatc
    15481 aacagcatcg gaatcgatgt gcaaaaaatt tacacgaccc actatcttcc caacgatttc
    15541 aagaaaaatc aaggctacaa acgttcagta gagatgtgtg aggagtatga tatctatcgt
    15601 caatgttatt gtggctgcgt ctatgcagcc caagcccaga atattgacct ggttcaagtt
    15661 aagaaggacg ctacagcatt cttgttgggt aaggatgttg agaaagacta ttctcatatc
    15721 aaatttata
//
LOCUS       DAVBDG010000045        14981 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBDG010000045 DAVBDG010000000
VERSION     DAVBDG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.046"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            15..599
                     /locus_tag="VQ526_001486"
     CDS             15..599
                     /locus_tag="VQ526_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET2555194.1"
                     /translation="MQYRNPDKAGAEREKMLAFRHKGQEVRKAFTELAKAFQASHPEW
                     QLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDFGISLEVSFIERKK
                     DEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLREKVRSQEVRKVLVK
                     VDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            730..2187
                     /locus_tag="VQ526_001487"
     CDS             730..2187
                     /locus_tag="VQ526_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2555195.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            2210..2491
                     /locus_tag="VQ526_001488"
     CDS             2210..2491
                     /locus_tag="VQ526_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2555196.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            2568..3053
                     /locus_tag="VQ526_001489"
     CDS             2568..3053
                     /locus_tag="VQ526_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2555197.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            3070..3735
                     /locus_tag="VQ526_001490"
     CDS             3070..3735
                     /locus_tag="VQ526_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2555198.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            3739..4602
                     /locus_tag="VQ526_001491"
     CDS             3739..4602
                     /locus_tag="VQ526_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2555199.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            4604..5308
                     /locus_tag="VQ526_001492"
     CDS             4604..5308
                     /locus_tag="VQ526_001492"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2555200.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            5487..7160
                     /locus_tag="VQ526_001493"
     CDS             5487..7160
                     /locus_tag="VQ526_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2555201.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            7272..8363
                     /gene="ulaG"
                     /locus_tag="VQ526_001494"
     CDS             7272..8363
                     /gene="ulaG"
                     /locus_tag="VQ526_001494"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2555202.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            8477..10453
                     /gene="tkt"
                     /locus_tag="VQ526_001495"
     CDS             8477..10453
                     /gene="tkt"
                     /locus_tag="VQ526_001495"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2555203.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            10570..10869
                     /gene="yajC"
                     /locus_tag="VQ526_001496"
     CDS             10570..10869
                     /gene="yajC"
                     /locus_tag="VQ526_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2555204.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            10915..11343
                     /locus_tag="VQ526_001497"
     CDS             10915..11343
                     /locus_tag="VQ526_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2555205.1"
                     /translation="MKKLVFVCMGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            11345..11755
                     /locus_tag="VQ526_001498"
     CDS             11345..11755
                     /locus_tag="VQ526_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555206.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            12055..14706
                     /gene="adhE"
                     /locus_tag="VQ526_001499"
                     /gene_synonym="adhC"
     CDS             12055..14706
                     /gene="adhE"
                     /locus_tag="VQ526_001499"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2555207.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         4676 a         2753 c         3198 g         4354 t
ORIGIN      
        1 tagactttgc tggtttgcag taccgtaatc ctgataaagc gggagcagag cgagagaaga
       61 tgctggcatt ccgccacaaa ggacaagagg tccgaaaggc ttttacagaa ctggccaaag
      121 cctttcaagc aagccatcca gaatggcaac tccaacagac tagccagtgg atgaatcagg
      181 cccagcgttt gagaccacat ttttgggttt atctacagag agacggacaa gtgacagaac
      241 ctatgatggc cttacgtttg tatgggacat ctactgactt tggaatttct ttggaagtca
      301 gtttcatcga acgtaagaag gatgagcaaa cactgggcaa gcaggccaaa gttttagaca
      361 ttccaaccgt taaagggatt tattatctaa cctactctaa tggtcaaagt caacggtggg
      421 aggcgaatga agaaaagcgt cgtactttac gcgagaaggt gagaagtcaa gaagttcgaa
      481 aagttttagt gaaggtagat gttcctatga cagaaaattc gtctgaagaa gaaatcgtag
      541 aaggcttatt gaagtcttat tctaaaattc ttccctatta tctagctacg agaaaataag
      601 ataatttgta aaacatcata aatcatacag tccaagagtg aacagtccgc tgtgtaattc
      661 ttggtctttt tgtttgcgct ttcgcattat ataataaact tacaaaaaca attcaaaagg
      721 agaacaatta tggaagtcgt ttcaagtgtt ctaaattggt tttctagcaa tattttgcag
      781 aatcccgcat ttttcgtagg tttattggtg ttgataggat atgcactttt gaaaaaacct
      841 gcccatgacg ttttttcagg gtttgttaaa gcaacagtag ggtatatgtt gcttaacgtg
      901 ggtgctggtg gtttggttac aacctttcgt ccaatcttag cagctcttaa ctacaaattc
      961 caaattggtg cagcggttat cgacccttac tttggacttg ctgcagcaaa caacaaaatt
     1021 gcagcagagt ttccagattt tgttggaact gcaactacag ctctattgat tggttttgga
     1081 ataaatatct tgctcgtagc tcttcgaaag attacgaagg taagaaccct ctttattact
     1141 ggtcacatca tggtacaaca agctgcaaca gtatctctta tggttctatt cttagtacca
     1201 caattgcgca atgcttacgg tacagcagcg attggtatca tctgtggact ttactgggca
     1261 gttagttcaa atatgactgt tgaggcaact caacgcttga ctggtggtgg cggatttgcg
     1321 attggtcacc aacagcaatt tgcaatctgg tttgtagata aagtagcagg acgctttggt
     1381 aagaaagaag aaagtttaga caatcttaaa ttacctaagt tcctctcaat cttccacgat
     1441 acagttgttg catctgctac cttgatgctc gtattcttcg gagccattct tttaatcttg
     1501 ggtccagaca ttatgtctaa taaagaagtc atcacttcag gaactctatt caatcctgct
     1561 aaacaagatt tctttatgta cattatccaa acagccttta ccttctcagt ttacttgttc
     1621 gttttgatgc aaggtgtccg aatgttcgta tctgagttga caaacgcctt ccaaggtatt
     1681 tcaaacaaat tgttgccagg ttcattccca gcggttgacg ttgcagcttc ttatggattt
     1741 ggttctccaa atgctgtctt gtcaggattt acctttggtt tgattggtca attgattaca
     1801 attgtcttgc tcatcgtctt taaaaatccg attcttatta ttacaggatt tgtaccagtg
     1861 ttctttgaca atgcagccat tgcggtctac gctgataaac gcggcggatg gaaagcggct
     1921 gttatccttt cctttatatc aggtgtcctt caagttgctc taggagctct ttgtgtggcc
     1981 cttctcgatt tggcatctta tggtggctac catggaaata tcgactttga attcccatgg
     2041 cttggatttg gatatatctt caaatacctt ggtattgttg gttatgtact tgtgtgtctc
     2101 ttcttgcttg ttattcctca acttcaattt gccaaagcaa aagataaaga gaaatattac
     2161 aacggtgaag ttcaagaaga agcttagtat ctagaaaagg agaaataaaa tggttaaagt
     2221 attagcagcg tgcggaaatg gaatgggttc atcaatggtt atcaagatga aggttgaaaa
     2281 tgctctccgt aagcttaatc aaacagattt tacagtcaat tcatgcagtg tcggtgaagc
     2341 taaaggttta gcagtaggat atgacatcgt aatcgcttct cttcatttga ttcaagaatt
     2401 ggaagggcga actaatggga agttaattgg tcttgataac ttgatggatg ataaagaaat
     2461 caccgaaaaa ctcagtcaag cactacagta aaaggttgga gggggctgga cagaaactga
     2521 gagttatcgt ttctgtcctt ctccctcttt aaataaagga ggcagatatg aatttaaaac
     2581 aagctttaat tgacaatgac tcgatccgac taggtttaga ggctaacaat tggaaagaag
     2641 cagtcaaggt agcagtagat cccctaattg aaagtggggc aattttgcca gagtattacg
     2701 atgctatcat tgaatcgact gaagagtatg ggccttacta tatcttgatg ccaggtatgg
     2761 ctatgcccca cgctagacct gaagctggtg tgcaaagtga tgccttttca ttgattacct
     2821 tacaaaatcc tgttgtattt tcagatggga aagaggtatc tgttttgttg gcactagcag
     2881 caacaagttc aaaaattcac acaagtgtag ccattccaca aattattgcc ctgtttgaat
     2941 tagaagattc tattgcacgt ttacaggctt gccagactaa agaagatgtc ttggctatga
     3001 ttgaagaatc taaggatagc ccttatctcg aaggattgga tttggaaagt tagaaagagg
     3061 aataaagaaa tgacaaaaag aatacctaat ttacaagttg cattagacca ttcagacttg
     3121 caaggagcga ttaaagcagc tgtttctgtt ggtcaggaag tagatattat cgaagctgga
     3181 actgtttgct tgcttcaagt tggaagtgaa ctggctgaag tcttgcgtag ccttttccca
     3241 gataagatta ttgtggcaga cacaaaatgt gctgatgctg gtggaacagt tgctaaaaat
     3301 aatgcggttc gtggagcaga ctggatgact tgtatctgtt gtgcaaccat ccctactatg
     3361 gaagcagctc taaaggctat caagactgaa cgaggagaac gaggcgaaat ccagatcgag
     3421 ctttatggcg attggacttt tgaacaagct cagctttggc tagatgcagg tatctcacaa
     3481 gctatttatc accaatctcg tgatgctctt cttgctggtg aaacttgggg tgaaaaagac
     3541 cttaataagg ttaaaaaact cattgacatg ggcttccgtg tatctgtaac aggtggtcta
     3601 gatgtagata ctctcaaact ctttgaaggt gttgatgtct ttacctttat cgcaggtcgt
     3661 ggaattacag aggctgcgga tccagcagga gcagcgcgtg ccttcaagga tgaaatcaaa
     3721 cgaatttggg ggtaaatcat ggtacgtcca attggaattt atgaaaaggc aaccccaaca
     3781 cactttactt ggctagaacg tttaaatttt gccaaggagt taggctttga ttttgtcgag
     3841 atgtctattg acgaacgtga cgagcgttta gcaagacttg actggagtaa ggaagaacgc
     3901 ttggaagttg tcaaagcaat ctatgaaact ggtgttcgta ttccttctat ctgtttttca
     3961 ggccatcgtc gctacccatt gggttcaaaa gatccagttc tagaggaaaa atctctagaa
     4021 ctcatgaaaa aatgtatcga attagctcaa gacttgggag ttcgtacgat tcaattagct
     4081 ggttacgatg tttactatga ggaaaagtca ccccagacac gccaacgttt tatcaaaaat
     4141 ttgagaaaag cctgtgactg ggctgaagaa gctcaggtgg tacttgctat tgaaattatg
     4201 gatgatcctt tcatcagtag catcgaaaaa tatttggcta tagaaaaaga gattgactct
     4261 cccttcctct ttgtatatcc agatattggt aatgtgtctg catggcataa tgatatctat
     4321 agtgagtttt atcttggtca tcatgccatc gcagctctcc atctcaagga tacttatgca
     4381 gtgacagaaa gttcaaaggg ccagttccga gatgtacctt tcgggcaagg ttgtgtcaaa
     4441 tgggaagaag ctttcgatat tttaaaggaa accaattata atggaccttt cctaatcgaa
     4501 atgtggtctg aaaattgtga aacagtagaa gaaacacgcg cagccgttca agaggcgcaa
     4561 gcttttctct atccactcat taagaaagca ggtttgatgt aagatgaatc aagtaatcaa
     4621 tgctatgcgt aaacgagtct gtgatgccaa tcaatcattg ccaaaacatg gacttgtcaa
     4681 atttacctgg gggaatgtat ctgaagttaa tcgcgaactc ggtgtcattg ttatcaaacc
     4741 atcaggcgtg gattatgacg aattgacacc tgaaaacatg gtagtgactg atctagatgg
     4801 taagatccta gaaggggatt taagaccatc ttccgacctc ccaactcatg tgcaattata
     4861 taaggcttgg tcagaaattg gtagtgtggt tcacacccat tcgacagaag ctgttggttg
     4921 ggctcaggca ggtcgtgata ttcctttcta cggaacaacc catgcagatt atttctacgg
     4981 ttcaatccct tgcgcccgta gtttgaccaa ggacgaagta gaagtggcct atgaaaaaga
     5041 tactggcctg gttatcgtag aagagtttga acatcgcgga cttaacccgg ttgaagtacc
     5101 aggaattgtt gtacgcaatc acggtccatt cacctggggc aaaaatccag agaatgctgt
     5161 ttatcactct gtcgtactag aggaagtatc aaagatgaat cgctttacag aacaaatcaa
     5221 tccaagagtt ggacctgctc cccagtacat actagaaaaa cactaccaac gtaaacatgg
     5281 accaaatgct tattatggtc aaaagtaaga acgatgaagg aggagaaaaa gataaattta
     5341 gctcctcttt ttacatttga tttttattga gagtaaagtt ggaattgaag taattttaaa
     5401 agatttttta gaaatagcgc ttgatatata tatggtaaaa taaaaagaat tgctgtgata
     5461 tcaatagatt tgggggattt tttaatatgg tactggataa ggcaagttgt gatttgcttc
     5521 aatatttgat ggatcaagaa acgtccaaaa cgattatggc gatttcgaaa gatttgaaag
     5581 agtcaagaag gaaaatttat tatcacattg acaaaatcaa tgctgctctg ggtgacgagg
     5641 cgcttcacat cattagtatt ccacgaattg gtattcactt aacggaagag cagagagatg
     5701 cttgttgtaa actattatcg gaagtagatt cgtacgatta tatcatgagt gcgcatgaac
     5761 gtatgatgat aatgttacta tggataggta tttctaaaga acgtattacg attgaaaaat
     5821 tgatagagtt aacagaggta tctaggaata ctgttctcaa tgatttgaat agtattcgtt
     5881 atcaactaac tttggaacaa tatcaggtga tcttgcaagt gagcaagtca cagggatacc
     5941 accttcatgc ccaccctctt aataaaattc agtatcttca atcgcttcta tatcatattt
     6001 ttatggaaga aaatgccact tttgtatcta ttttagaaga taagatgaaa gagaggttag
     6061 atgatgagtg tttgctttct gttgaaatga accaattttt taaggaacag gttcctttag
     6121 ttgaacaaga tttagggaag aaaataaacc atcatgaaat aacttttatg ttgcaggttc
     6181 taccttattt gctgttaagc tgtcataatg ttgaacagta tcaagaaaga catcaggata
     6241 tagagaaaga attttctttg ataagaaaaa gaatagagta tcaggtgtct aagaaattag
     6301 gagaacggtt gtttcaaaag tttgaaattt ctttgtcagg acttgaagtt tctcttgtag
     6361 ctgttctcct cctctcctat cgtaaagatt tggatattca tgcagaaagt gatgattttc
     6421 ggcaattaaa acttgcttta gaagaattta tctggtattt tgaatcacaa atccgaatgg
     6481 agattgagaa caaggatgat ttgttacgaa atttgatgat ccactgtaaa gccttgttat
     6541 ttagaaagac ttacggtatt ttttctaaaa atcctctaac aaaacaaatt cgatccaagt
     6601 atggagaatt atttttagtc actagaaaat ctgcggaaat tttagaagga gcatggttta
     6661 ttcggctaac agacgatgat attgcctatt tgacgattca tattggagga tttttaaaat
     6721 atacaccatc gtctcaaaaa aatatgaaaa aagtttatct cgtttgtgat gaaggtgttg
     6781 cggtttcgag acttttgctg aaacaatgca aactttattt tccaaatgag caaattgaca
     6841 ctgtatttac aacagaacaa tttaagagtg tggaagatat tgcacaagtt gatgtagtga
     6901 ttactactaa tgatgatttg gatagcagat ttccgatttt aagggttaat cctatccttg
     6961 aagcagaaga tattttgaaa atgctagact atcttaaaca caatatattt cgtaataaga
     7021 gcaaaagttt cagtgaaaat ctttctagtc ttatttcgtc ttatattgta gatagcaagt
     7081 tggctagtaa gttccaagaa gaggttcaaa cacttataaa tcaagaaata gtagttcaag
     7141 cttttttgga agatatttga aggacagtcc aatgatgaac acaaacctgt gtttttcttg
     7201 gtctttttta gtgttttgaa gggtggtata ctaatctcaa agataacaat tatatccaaa
     7261 ggaggcaaca tatgccaaac gtcaaagaaa ttacaagaga gtcatggatt ttagccactt
     7321 tcccagagtg gggaacatgg ttgaacgaag aaatcgaaga agaagtcgta cctgaaggca
     7381 actttgccat gtggtggcta ggcaactgtg gtacttggat taagacacca gctggtgcta
     7441 acgttgtcat ggacctttgg tcaaaccgtg gaaaatcaac caaaaaagtg aaagatatgg
     7501 ttcgtgggca ccaaatggca aatatggcag gtgttcgtaa gttgcaacca aacttgcgtg
     7561 ttcagccaat ggttatcgat ccatttgcta tcaacgaact agactattac ttagtttcac
     7621 acttccacag tgatcatatc gacccataca cagctgcagc aattctcaac aatcctaagt
     7681 tagagcatgt taagtttatc ggtccttacc actgtggacg aatctgggaa ggatggggtg
     7741 ttccaaaaga acgtatcatc gttgttaaac caggtgacac tatcgaatta aaagatatga
     7801 agattcatgc agtagaatca tttgaccgta cttgcttggt aactctccca gtgaacggtg
     7861 ctgatgagac aggcggtgaa cttgctggct tggctgttac agatgaagaa atggctcaaa
     7921 aggctgttaa ctatatcttt gaaacaccag gtggaaccat ctatcatggt gcagattctc
     7981 acttctcaaa ctattttgca aaacatggta aagactttaa aattgatgtt gctttgaata
     8041 actatggtga aaatccggta ggtatccaag acaaaatgac atctatcgac cttcttcgta
     8101 tggcagaaaa tctgcgtacc aaagtcatta tcccagttca ctatgatatc tggtctaact
     8161 tcatggcttc tactaatgag attctagaac tttggaaaat gcgaaaagat cgcttgcaat
     8221 acgatttcca tccatttatc tgggaagttg gcggtaagta cacttatcct caagatcaac
     8281 acttagtaga ataccatcat ccacgtggtt ttgatgattg ttttgaacaa gactctaaca
     8341 ttcaatttaa agctttgcta taatataaaa atattctttg gaggttatcc gtatgttgca
     8401 tgatacggat aattttcata taatagtaaa atagaatgtg tgattcaata atcacctcaa
     8461 atagaaagga aattctatgt caaatctatc tgttaatgca attcgttttc taggtattga
     8521 cgccattaat aaagccaact caggtcatcc aggtgtggtt atgggagcgg ctccgatggc
     8581 ttacagcctc tttacaaaac aacttcatat caatccagct caaccaaact ggattaaccg
     8641 cgaccgcttt attctttcag caggtcatgg ttcaatgctc ctttatgctc ttcttcacct
     8701 ttctggtttt gaagatgtca gcatggatga gattaagagt ttccgtcaat ggggttcaaa
     8761 aacaccaggt cacccagaat ttggtcatac ggcagggatt gatgctacga caggtcctct
     8821 agggcaaggg atttcaactg ctactggttt tgcccaagca gaacgtttct tggcagccaa
     8881 atataaccgt gaaggctaca atatctttga ccactatact tacgttatct gtggagacgg
     8941 agacttgatg gaaggtgtct caagcgaggc agcttcatac gcaggtttgc aaaaacttga
     9001 taagttggtt gttctttatg attcaaatga tatcaacttg gatggtgaga caaaggattc
     9061 ctttacagaa agtgttcgtg accgttacaa tgcctacggt tggcatactg ccttggttga
     9121 aaatggaaca gacttggaag ccatccatgc tgctatcgaa acagcaaaag cttcaggcaa
     9181 gccatctttg attgaagtga agacggttat tggatacggt tctccaaaca aacaaggaac
     9241 taatgctgta cacggcgccc ctcttggagc agatgaaact gcatcaactc gtcaagccct
     9301 cggttgggac tacgaaccat ttgaaattcc agaacaagta tatgctgatt tcaaagaaca
     9361 tgttgcagac cgtggcgcat cagcttatca agcttggact aaattagttg cagattataa
     9421 agaagctcat ccagaactgg ctgcagaagt agaagccatc atcgacggac gtgatccagt
     9481 cgaagtgact ccagcagact tcccagcttt agaaaatggt ttttctcaag caactcgtaa
     9541 ttcaagtcaa gatgccttga atgttgtggc agctaaatta ccaaccttcc taggtggatc
     9601 agctgacctt gctcactcaa acatgactta tatcaagacg gatggacttc aagacgatac
     9661 caatcgcttg aatcgtaaca ttcagtttgg tgttcgtgaa tttgcaatgg gaacaatctt
     9721 gaacgggatg gcccttcatg gtggacttcg tgtatacggt ggaactttct tcgtcttctc
     9781 tgactatgtg aaggcagctg tccgcttgtc agccttacaa ggacttcctg tgacttatgt
     9841 ctttacccat gattcaatcg cagttgggga agatggtccg actcacgaac cagttgagca
     9901 tttagcaggt cttcgtgcta tgccaaatct aaatgttttc cgtccagcag atgcgcgtga
     9961 aacgcaagca gcttggtacc ttgcagtgac aagtgagaaa acaccaactg cccttgtctt
    10021 gacacgtcaa aacttgactg ttgaagatgg aacagacttc gacaaggttg ctaaaggtgc
    10081 ttatgttgta tatgaaaatg cagccgactt tgataccatc ttgattgcga caggttcaga
    10141 ggttaatctt gctgtctcag ctgctaaaga attggctagt caaggcgaaa aaatccgcgt
    10201 agtcagcatg ccatctacag atgtctttga taaacaagat gcagcttaca aggaagaaat
    10261 tcttccaaat gcagtccgcc gtcgtgttgc agtcgaaatg ggtgcaagtc aaaactggta
    10321 caaatatgtt ggtctcgatg gtgccgttct aggtattgat accttcggag cctctgcccc
    10381 agcaccaaaa gtattggcag aatatggctt tactgtcgaa aatcttgtaa aagttgttcg
    10441 aaatttgaaa taatcctaaa aatcagggcg taagctctgg tttttcttac cagaaaagta
    10501 aggtacaatc ttgtaaaagt agctgaaatt tgatatagta gtcctatgta aaagacaaag
    10561 gagaaaacaa tgaatccaaa tattactttt ttaatcatgc ttgtaggtat gatggccttg
    10621 atgttcttta tgcaacgttc tcaaaagaaa caagctcaaa agcgtatgga aagcttaaat
    10681 aaactacaaa aaggctatga agtgattaca atcggtggac tttatggaac agtcgatgaa
    10741 gtagatacgg agaagggaac aatagttctt gacgtagatg gagtttactt gacttttgaa
    10801 ctagctgcta tcaagacagt attaccgctg aaagaaacag cttcactaga aggcgcaatt
    10861 gaaaaataag acgggatcac gaactcccgt tttctataaa agaaaggaaa tgggatgaaa
    10921 aaattagtct ttgtctgtat gggaaatatt tgccgtagcc ctatggccga gtttgttatg
    10981 aaatcaatga cagataacta cgaaatccaa agtcgagcaa cttcctcttg ggaacatggc
    11041 aatccgattc ataaggggac tcagggaatt tttcaagagt atgagattcc ttatgacaag
    11101 aacaagacat cgcttcagat tagtaaggaa gattttgaag cctttgatta tattatcgga
    11161 atggacgctt caaatatttc tgacttacgt cagatgtgtc cagtagactg tcaagataag
    11221 atttactcat tttcatctga aagtgttcca gacccttggt atacaggaga ttttgaagaa
    11281 acctatcgac gtgttcaaga gggctgtcaa gcttggttag aacgtttaga aaaggagagt
    11341 taaggtggaa aatcttacga atttttacga aaagtatcgt gtctatctga ctcgtccacg
    11401 tttagagctt ttggcagtag ttaccattgt tttctgtgct gtactcgtct tttttctaaa
    11461 tattccagga aaaggtgtct taaaactcga taatggaacg attgtttatg atggcagtct
    11521 tgtccgtggt aaaatgaatg gccaaggtac cattaccttc caaaatggag accaatatac
    11581 aggtggcttc aacaatggag ccttcaacgg aaaaggtacc tttcaatcta aagaaggctg
    11641 gacctacgaa ggtgattttg taaatggtca ggctgaagga aaagggaaac taacaacaga
    11701 acaagaagtc gtttatgaag gaacttttaa acaaggcgtt tttcaacaaa aataaagcct
    11761 ccttatcaaa ggaggtatta ttagaatcac aaggtaagcg cttacctgtg aatccctttc
    11821 tttccaaatc cctcttccaa gcaagtttgt gaaataaaaa atatttgaaa taaatttcac
    11881 aaacttcaaa gataaaactt gataagaaaa gaaaatgaga aaagtttcac aagagtttaa
    11941 aaattctttg tgaaatgttt atgaaactgt ttacaaagtt gaaataatgc gttataataa
    12001 acttgtgaaa aaattaacaa aggatatcgt tccttgaaag ctatggagga aaatatggct
    12061 gataaaaaaa ctgtgacacc agaggaaaag aaactcgttg ctgaaaaaca cgtagatgag
    12121 ttggttcaaa aagctctagt tgcccttgaa gaaatgcgta aattggatca agaacaagtt
    12181 gactacatcg ttgccaaagc atcagtagca gctttggatg cccacggaga attggcttta
    12241 catgcctttg aagaaacagg acgtggtgta tttgaagaca aagcaactaa gaacttgttt
    12301 gcttgtgaac acgtagtaaa caacatgcgc cacactaaga cagttggcgt tatcgaagaa
    12361 gacgatgtaa caggattgac tcttattgct gaaccagttg gtgttgtttg tggtattact
    12421 ccaacaacaa acccaacatc aacagcaatc ttcaaatcat tgatttcatt gaagacacgt
    12481 aacccaatcg tctttgcctt ccatccatca gcacaagaat catctgctca tgcagctcgt
    12541 atcgtccgcg atgcagctat cgcagctggt gctcctgaaa actgtgtaca gtggatcact
    12601 caaccatcta tggaagcaac tagtgccctt atgaaccacg aaggtattgc aacaatcctt
    12661 gcaacaggtg gtaatgccat ggttaaagcc gcatactcat gtggtaaacc agctcttggg
    12721 gtaggtgccg gaaacgttcc agcttatgtt gaaaaatcag caaacattcg tcaagcagca
    12781 cacgatatcg tcatgtctaa atcatttgat aacggtatgg tctgtgcatc tgaacaagca
    12841 gttatcattg ataaagaaat ttacgatgaa tttgtagcag agttcaaatc ttaccacact
    12901 tactttgtaa acaaaaaaga aaaagctctt cttgaagagt tctgcttcgg cgtcaaagca
    12961 aacagcaaaa actgtgctgg tgcaaaattg aacgctgaca tcgttggtaa accagcaact
    13021 tggattgcag aacaagcagg atttacagtt ccagaaggaa caaacattct tgctgcagaa
    13081 tgtaaagaag ttggcgaaaa tgagccattg actcgtgaaa aattgtcacc agttattgca
    13141 gttttgaaat ctgaaagccg tgaagatggt attactaagg ctcgtcaaat ggttgaattt
    13201 aacggtcttg gacactcagc agctatccac acagctgacg aagaattgac taaagaattt
    13261 ggtaaagctg ttaaagctat tcgtgttatc tgtaactcac cttctacttt tggtggtatc
    13321 ggggacgttt acaatgcctt cttgccatca ttgacacttg gatgtggttc ttacggacgc
    13381 aactcagttg gggataacgt tagtgccatt aacctcttga atatcaaaaa agtcggaaga
    13441 cggagaaata acatgcaatg gatgaaactt ccttcaaaaa catactttga acgtgattca
    13501 attcaatacc ttcaaaaatg tcgtgacgtt gaacgtgtca tgatcgttac tgaccatgcc
    13561 atggtagagc ttggtttcct tgatcgtatc atcgaacaac tggaccttcg tcgcaataag
    13621 gttgtttacc aaatctttgc ggatgtagaa ccggatccag atatcacaac tgtaaaccgt
    13681 ggtactgaga ttatgcgtgc cttcaaacca gataccatca tcgcactcgg tggtgggtct
    13741 ccaatggatg ctgccaaagt aatgtggctc ttctacgagc aaccagaagt ggacttccgt
    13801 gaccttgtcc aaaaattcat ggatatccgt aaacgtgcct tcaagttccc attgcttggt
    13861 aagaagacta aattcatcgc gattccaact acatctggta caggatctga agtaacacca
    13921 tttgccgtta tctctgataa agcaaacaac cgtaaatacc caatcgctga ctactcattg
    13981 acaccaactg tggcaatcgt agatcctgct ttggtattga cagttccagg atttgttgct
    14041 gctgatactg gtatggacgt attgactcac gcgacagaag catacgtatc acaaatggct
    14101 agtgactaca ctgatggttt agcacttcaa gctattaaat tggtctttga aaatctcgaa
    14161 agctcagtta aaaatgcaga cttccactca cgtgagaaaa tgcataacgc ttcaacaatc
    14221 gctggtatgg cctttgccaa tgccttccta ggtatttctc actcaatggc ccataagatt
    14281 ggtgcgcaat tccacacagt tcacggacgt acaaatgcca tcttgcttcc gtacgttatc
    14341 cgttacaacg gtacacgtcc agctaagaca gcaacatggc ctaagtacaa ctactaccgc
    14401 gcagatgaaa aataccaaga tatcgcacgc atgcttggac ttccagcttc tactccagaa
    14461 gaaggggttg aatcttacgc aaaagctgtc tacgaactcg gtgaacgtat tgggatccaa
    14521 atgaatttta gagaccaagg aattgacgaa aaagaatgga aagaacattc tcgtgaatta
    14581 gccttcctgg cttatgaaga ccaatgttca ccagctaacc cacgtcttcc aatggtagac
    14641 catatgcaag aaatcatcga agatgcatac tatggctaca aagaaagacc aggacgccgt
    14701 aaataattgt ttatcagtct agaagcaaga caaaaactca atttgaggga aagatccagt
    14761 aatttttcta tgataaaagg catcctatca aggtttttga acacctgata ggatgccttt
    14821 ttatgatatt gaggcctttt tgcccttttt gaaaaactag aatagaaaca aaatatataa
    14881 tagattgaaa ctagagtagt acacatctgc ttctaaaaca ttgttagaat tcgatttgac
    14941 tgtcctgatc aatttgtcct gttcttattt cattttaata t
//
LOCUS       DAVBDG010000046        14965 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBDG010000046 DAVBDG010000000
VERSION     DAVBDG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.047"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(26..628)
                     /locus_tag="VQ526_001500"
                     /pseudo
     CDS             complement(26..628)
                     /locus_tag="VQ526_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(1214..1990)
                     /locus_tag="VQ526_001501"
     CDS             complement(1214..1990)
                     /locus_tag="VQ526_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2555208.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            2112..2858
                     /locus_tag="VQ526_001502"
     CDS             2112..2858
                     /locus_tag="VQ526_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2555209.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2871..3851
                     /locus_tag="VQ526_001503"
     CDS             2871..3851
                     /locus_tag="VQ526_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2555210.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            4046..4366
                     /locus_tag="VQ526_001504"
     CDS             4046..4366
                     /locus_tag="VQ526_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2555211.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            4412..4720
                     /locus_tag="VQ526_001505"
     CDS             4412..4720
                     /locus_tag="VQ526_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2555212.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            4737..6035
                     /locus_tag="VQ526_001506"
     CDS             4737..6035
                     /locus_tag="VQ526_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2555213.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            6186..8624
                     /locus_tag="VQ526_001507"
     CDS             6186..8624
                     /locus_tag="VQ526_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2555214.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            8643..9311
                     /locus_tag="VQ526_001508"
     CDS             8643..9311
                     /locus_tag="VQ526_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2555215.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            9329..10417
                     /locus_tag="VQ526_001509"
     CDS             9329..10417
                     /locus_tag="VQ526_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2555216.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            10872..13373
                     /locus_tag="VQ526_001510"
     CDS             10872..13373
                     /locus_tag="VQ526_001510"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2555217.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEIIAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKVEIDGKEIVKVISVPNKLVNIVVK"
     gene            13567..14262
                     /locus_tag="VQ526_001511"
     CDS             13567..14262
                     /locus_tag="VQ526_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555218.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            14274..14690
                     /locus_tag="VQ526_001512"
     CDS             14274..14690
                     /locus_tag="VQ526_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555219.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
BASE COUNT         4796 a         2331 c         3103 g         4735 t
ORIGIN      
        1 aatttgtcct gttcttattt cattttacta taccatcaat aagtgtgaaa agatctttta
       61 cataagacca agccttatta tctttgtaac caccttgagc ttgtagcata tttgttaatt
      121 gagcaaaggc gctagaagag tttgctgggt cagcagttgc gatttttcct tttagttcag
      181 gtttgagaag gtcgctatat ccttcgatgt tcatgccttt agttaaatca gggtggacga
      241 ttaaaacact accatctagt gtataaggag tagagaagcc agttgtgttt tgatattctt
      301 tgataacatt atcattttct tttgaaatat agttttcaaa gagttctccg tgggtagcat
      361 attgtgtata agaaccacca aagataacat cagctacagg aacttctttt tctgactcta
      421 gttttttgaa aagttctcca gtaccagctt gaatcagttc tattttgata ccatattttt
      481 cttcaaaggc aggaatagtt gctccaatta agccttctga gtttggtgaa taaacgacta
      541 gcgaaccacc gtctccttta tcagatgaac tgtcatcggc agattcatta gaagaacaag
      601 cagcataata catccatttc tttttcatga tggatacctc cgttgtgtta tttaagttta
      661 ttttaaaaca atgtaagcgt ttttaaaacc tacaattcta ttctatagtg tattgaatct
      721 ataacagtac accttgactg ctaaaatatt tctataaatt aatttgactt tcctgataga
      781 gatgttcaca tcttatttca attcactata ttagagtaaa attctctaca aaaaaaagaa
      841 tagcctattt tactattttt ccgagtgatt tcaattcctt tggggaaata tggagatact
      901 ttttaaatcc tgacaaatgg ttgtttcttt ttctaaatcg gtgatactgt atcggagaat
      961 gcgcgtgagg tcacaaaggc tgcgatagag cttctatgga gaatttcttt ttggagagat
     1021 tttttaaagg aatgagacat ccgctacctc cttggaaggt tttttgtgat aagtatagca
     1081 gtttaaaaat tctagagcaa ggtaaaatag aaaaggtttg gctaaaattt tttagctaaa
     1141 ccttttgaaa agttaagtat gtattataga tgcttgttat ctgcaaatat ctagagcatt
     1201 tcaaggaaat aaactagaaa taacaattaa tatggtggtt cagaaatacc ttttgataat
     1261 caataagatc ttcaggatga agtgcgttga ctccatccat ttcatatttc cgatttaata
     1321 aacgatattt gttttcacca aattgatgaa aagggagtag ttgaacttgg tcgatattta
     1381 atgagttaaa tagagtagcg aatttttctg catcctctaa actattgtta aaattaggaa
     1441 taactgggat tcttaaaacg atagttttat tttgagaaaa agcataatga atgtttttaa
     1501 taatcatttg attaaaaacc ccagtcactt ttttatgttt tatagaatta taatgtttta
     1561 ggtctgtgta gataaaatcc acatattgaa ttaaatcgat aaatttttca tgatcaacaa
     1621 aggcagtagt ttcaatggca gtgtgtatgt gatgttcttt agctgatttt aagatggctt
     1681 tagcaaattc aaactgagca aatatttcac ctcctgataa agttaaacct ccaccggatt
     1741 cttcgtaaaa ttctttgtct tttaatacct ctgtaataat ttcttctaca gtcttttctt
     1801 caccgactaa ggtgaatttc tctcgttgag catctttcat tttttcaggt ttcattcttt
     1861 gagattcagg attagaacac catggacagc gcagaggaca tccttttaaa aaaacagttg
     1921 tacgaatacc cgggccgtca tgaattgaaa agtgttgaat attaaaaata attcctttag
     1981 atatttccat aagagttcct cctcttcata agtctatcct atcacaaaaa cgaaagaaaa
     2041 acaattacga acgaaaggtt aatttgttgt tttttgcttt ttattttggt agaataaaac
     2101 ggaggtgtaa aatgaaaaga ttggaacaaa ttattaaatt agtatcagaa tatgaaaaga
     2161 tcgacgttaa tacattatcg gaaaaattaa atgtatcgaa agtaacgatt agaaaagatt
     2221 tagataaatt agagtcaaaa ggtttattac acagagagca tggatatgct gtattaaata
     2281 gtggagatga cttaaatgta cgtttgtcaa ttaattatga aattaagaga aaaattgttc
     2341 aggaagcagt aaaattggtg tcagataatg aaacaataat gatagaatct ggatcgacct
     2401 gtgctttact tgctgaggaa atttgcaagc aaaaaagaaa tgttacgatt gtaacaaatt
     2461 cattttttat agcaaatttt gtgagagctt atgattcatg tcgtattatt gttcttggtg
     2521 gagagtttca gaaggattca caggttactg tgggaccttt attaaaagaa atgatacaga
     2581 cttttcatgt gcgtcaagct tttgttggga cagatggcta cgataaagag atgggcttta
     2641 caggaaaaga tttaatgcgc agtgaggtag ttcaatatat ttcagcagcg tcggataaag
     2701 tcattgtact aactgactca agtaaatttg ataaaagggg tacagtaaga agatttgctt
     2761 taagtcaagt ctatgaagta ataacagacg aaaaactttc taaacaaaat atagctacat
     2821 tagaaaatgc tgggataatg gttaaggtag tttcgtaaga ggttaagtgt atgaatcaag
     2881 ataggaataa actgctctct aaaattgctt atctgtatta tattgaaaac ttaaatcagt
     2941 cacaaatagc agcaaaatta ggaatttata gaacctctat tagtagaatg ttaacagaag
     3001 caaggaatgc aggaattgtt aaaattgaaa tagagaattt tgataccaat atgtttaagt
     3061 tggaaaatta tgtaaaagaa aaatatggtc tggaaagttt agaaattatt ccaaatgaat
     3121 ttgatgataa tccaacaatt ttatctgaaa gaatttctca agttgcagca ggtgtcctta
     3181 ggaatctaat tgatgataat atgaaaattg gcttttcttg ggggaaaagt ttaagtaatt
     3241 tagtagattt aattcacagt aaaagtgtcc gaaatgttca cttctatcct ctagcaggtg
     3301 gtcctagtca catacacgct aaataccatg tgaatacact gatttatgaa atgtctagaa
     3361 aatttcatgg agagtgtaca tttatgaatg caacgattgt gcaagaaaat aaattgttag
     3421 cagatggtat tttgcaatca agatattttg aaaatttgaa aaatagttgg aaagatttag
     3481 atatagctgt agtcggaatt ggtgatttta gcaataaagg aaagcatcaa tggttagaca
     3541 tgcttacaga ggatgacttt aaagaattga ccaaagttaa aactgtagga gaaatttgtt
     3601 gtcgattttt tgattcaaaa ggtaaagaag tctatgaaaa cttacaggaa agaacgatag
     3661 caatctcttt ggaagattta aaaaatattc ctcaaagttt agctgttgct tacggtgata
     3721 cgaaagtatc ttcgattctt tctgtcttgc gtgctaattt agtaaatcat ttgattacag
     3781 acaaaaatac aattttaaaa gttttggaag aagatgggga tttgactttt agagagattc
     3841 taggtgagtg aaaatgatag actgattcag tttatcgttt ttctttttag ttgattgcac
     3901 atttgtgctt atataaacaa aaatagttta tctgttgttt ttggattgac aactttatta
     3961 tgtagttgta ttctatagtt acaaaagaaa attttaaaat ttcaaatgaa aaaagctttt
     4021 tacatagtga aatgaggagg aatttatgga aattattgtt ccagatcaaa ttatcatggg
     4081 tttaatttta tatgctggcg atgcgaaaca acatatttat aaagcattag attacataaa
     4141 gaacggtaca tgtgaacgat gtgaagaaga aatccagtta gctgatgccg ccttattaga
     4201 agctcataat ctacaaacaa aattcttggc acaggaagcg tctggtacaa agacagaaat
     4261 tacagctctc tttgttcatt cacaagatca tctcatgact agtatgacgg agattaattt
     4321 aatcaaagaa attattagtt tgagaaaaga acttcataaa aaataatact agagtattat
     4381 cattgttatt aacatagagg aggaaaacat aatggtgaag attggtttgt tttgtgcagc
     4441 aggtttttct actggtatgc ttgtaaataa tatgaaaatt gcagcgcaag ctagtggagt
     4501 tgaggcagaa atagaggcgt tttctcagtc taaattagcg gattatgcgc caaatataga
     4561 tgttgcacta ttgggtccac aagttgctta tacattagat aaatcaaaag aaatttgtga
     4621 taagtgtgat gttccgatag ctgttattcc gatgatggac tatggtatgt tagatgggaa
     4681 aaaagtatta gatttggccc tatctttgat tagtgggtaa gaaaaggaga tttattatgt
     4741 caaagatgga tgttcagaaa atcattgcac cgatgatgaa gtttgtgaat atgcgtggca
     4801 ttatagctct aaaagatggg atgttagcaa ttttgccatt gacagtagtt ggtagtttgt
     4861 tcttgattat gggacaattg ccgttcgaag gattaaataa gagcattgct agtgtttttg
     4921 gagctaattg gacagagccg tttatgcaag tatattcagg aacttttgct attatgggtc
     4981 taatttcttg tttttcaatt gcctattctt atgctaagaa tagcggagta gaggctttac
     5041 cagctggagt tctatctgta tctgcattct ttattttgct aagatcatct tatatcccta
     5101 aacaaggtga ggcgattggg gacgctatta gtaaagtttg gtttggaggc caaggaatta
     5161 tcggtgctat cattataggt ttggtagtag gaagtattta taccttcttt ataaagagaa
     5221 aaattgttat taagatgcca gaacaagttc cacaagctat tgccaaacag tttgaagcaa
     5281 tgattccagc atttgtaatt ttcttatctt ctatgattgt atatatttta gcgaagtcat
     5341 tgactaatgg cggaacattc atagaaatga tttattctgc tattcaagtt ccgttgcaag
     5401 gtttaactgg atctttgtat ggtgctattg gaattgcatt ctttatatca tttttgtggt
     5461 ggtttggtgt tcatgggcaa tcggtagtaa atggagtagt gacagctctg cttttatcta
     5521 atcttgacgc taataaagct atgttagcct ctgctaatct atcattagaa aatggtgcac
     5581 atattgttac tcaacaattt ttagattcat ttttaattct atcaggttca gggattacgt
     5641 ttggtcttgt agttgccatg ctttttgcag caaaatcaaa acaataccaa gccttaggaa
     5701 aagttgcagc ttttccagca atatttaacg taaatgagcc agttgtattt ggatttccga
     5761 ttgtcatgaa tccagttatg tttgtacctt tcattcttgt tcctgtactt gcagctgtga
     5821 tagtatatgg agctattgca acaggtttca tgcagccatt ctcaggggta acattgcctt
     5881 ggagtacacc agctattcta tcaggatttt tggtgggtgg atggcaaggt gttgtgactc
     5941 agttattagt attggtgata tctacagtgg tttactttcc attctttaag atacaagatc
     6001 gtttagctta ccaaaatgaa atcaaacaat cttagaggta tttgtgtgtt actgttaaac
     6061 tcacacattt gtgctaaaaa ttagagagtt aaaatttttc tagttaaaag cttgaaaatt
     6121 tctataaaaa tcggtattat attttcgaaa gaaataaaaa tattttcgaa agaaaggtgc
     6181 ttacgatggt aaatacagaa gtagcaagaa caacaatcaa gacagaatat tttggcagcc
     6241 ttactgaaag gatgaacaaa tatcgagaag atgttttaaa taaaaaacct tatattgatg
     6301 ctgagagagc agttctagca acacgcgcct atgaacgata caaggaacaa cctaatgtcc
     6361 taaaacgtgc atatatgctg aaagaaattt tggaaaatat gtctatctat attgaagaag
     6421 aatctatgat tgcgggaaat caagcttctt ccaataaaga tgctcctatt tttccggaat
     6481 atacgctaga atttgttctc aatgagttgg atctttttga aaagcgtgat ggagatgttt
     6541 tctatattac agaagaaaca aaagaacaac ttagaagtat tgctccgttt tgggaaaata
     6601 ataatttacg tgctagagct ggtgccttat tacctgaaga agtgtcagtt tatatggaaa
     6661 caggattctt cggtatggaa ggtaagatga attctggaga tgctcactta gcagttaact
     6721 atcagaaact tttgcaattt ggtttaagag gttttgaaga gcgggctcgt aaagcaaaag
     6781 tagctctaga tttaacagat ccagcaagta ttgataaata tcatttttac gactctatat
     6841 ttattgtaat cgatgctatt aaagtatatg caaagcgctt tgttgctctt gctaaaagtt
     6901 tagccgaaaa tgcaaatcct aaacgtaaga aagaattact tgagattgca gatatttgct
     6961 ctagagtccc atatgaaccg gcaactactt ttgcagaagc tattcaatca gtttggttta
     7021 ttcaatgtat tttacaaatt gaatctaatg gccactctct ttcatatggc cgttttgatc
     7081 aatatatgta tccatatatg aaggctgatt tagaaagtgg taaagaaaca gaagatagca
     7141 ttgttgaacg tctgacaaat ctttggatta agacaattac aattaataag gttcgcagtc
     7201 aatcacatac attttcttca gcaggaagtc ctttatatca aaatgttaca attggtggac
     7261 agactcgaga taagaaggat gctgttaacc cattatctta tttggtatta aaatcagttg
     7321 cacaaaccca tctaccgcaa cctaatctaa ctgtacgtta ccatgcaggt ttagatgctc
     7381 gtttcatgaa tgagtgtatt gaagtgatga aacttggttt tggtatgcct gcatttaata
     7441 atgatgagat tattattcct tcttttattg caaaaggagt attggaagat gatgcttatg
     7501 attacagtgc cattggatgt gttgaaacgg cagttccagg gaaatggggc tatcgttgca
     7561 caggtatgag ttatatgaac ttccctaagg ttctacttat cacgatgaat gatggaattg
     7621 atccggcttc gggtaaacgg tttgcaccaa gctttggtca ttttaaggat atgaagaact
     7681 tttctgaatt agaaaatgct tgggataaaa cactaagata tttgacacga atgagtgtta
     7741 ttgttgaaaa ttctattgat ttatcattgg aacgagaagt tcctgatatt ctatgttcag
     7801 cattgactga tgattgtatt ggtcgtggaa aacaccttaa agaaggtgga gcagtatatg
     7861 attatatatc aggattgcaa gttggaattg caaatttgtc ggattcatta gctgcaatta
     7921 aaaaattggt gtttgaggaa gaacgtataa gcccaagtca gctttggcat gcactggaaa
     7981 cagattatgc cggagaagaa ggtaaggtca ttcaagaaat gttgattcat gatgcaccta
     8041 agtatggtaa tgatgatgat tatgctgaca aattggttac tgctgcttat gacatttatg
     8101 ttgatgaaat tgctaaatat cctaatacac gttatggaag agggcctatt ggaggaattc
     8161 gttattcagg aacatcttct atatcagcca acgtagggca gggacgtgga acattagcaa
     8221 ctccagatgg acgcaacgcg ggtacaccgt tagcagaggg ttgttcacca tcacataata
     8281 tggatcaaca cggccctaca tctgttttaa aatctgtttc aaaattacca acagatgaaa
     8341 tcgtaggtgg ggttctctta aatcagaaag taaatcctca aacgttagcc aaagaagaag
     8401 ataaattaaa actaattgct ttgttacgaa cattctttaa tcgtttacat gggtaccata
     8461 ttcaatacaa tgttgtttcc agagagacgt tgattgacgc tcagaaacat cctgaaaaac
     8521 acagagactt aattgttcgt gttgcaggat actctgcatt cttcaatgtt ctttctaagg
     8581 caacccaaga tgacattata ggacgtactg agcatacttt gtaaaataaa gaggttcttt
     8641 ttatggaatt tatgcttgac acattaaatt tagatgagat taaaaagtgg tctgaaattt
     8701 tgccgctagc tggggtaact tcaaatccca ctattgcaaa aagagagggt tctattaatt
     8761 tttttgaacg aatcaaagat gtaagagaat tgattggctc cacaccctct attcatgttc
     8821 aggtgatttc tcaagatttt gaaggcatct taaaggatgc tcatgaaatt cgaagacaag
     8881 caggagatga tatatttatc aaagtacctg ttactccagc tggattacgt gcaataaagg
     8941 tgctaaaaaa agagggctac catatcactg caacagctat ttatacagtt attcagggat
     9001 tattagctat cgaagtagga gcggattacc tagctccata ttataataga atggaaaatc
     9061 tgaacattga ttcaaattct gtcattcgtc aattagctct tgctattgat agacagaact
     9121 ctcctagtaa gattttagct gcatccttta aaaatgtagc acaagtaaat aatgctttag
     9181 ctgcaggtgc gcatgctgtt acagcaggag cagatgtttt tgaatcagct ttcgccatgc
     9241 catctatcca aaaggcggtt gatgattttt ctgacgattg gtttgttact caaaatagtc
     9301 gttccattta gatagagagg aaatacatat gagaattttt gctagtcctt ctagatatat
     9361 tcagggggaa aatgccttgt ttgaaaatgc caaatcaatt ttggatttgg gaaattaccc
     9421 gattctatta tgcgatcagt tggtttatga tattgttgga aaacgatttg aagattatct
     9481 acataggtat ggtttccata ttgttctggc gctatttaat ggtgaagctt ctgacaatga
     9541 aatcaatcga gttgttgcct tggctgagaa agaaaattgt gatagtatta tcggtcttgg
     9601 tgggggaaag acgattgata gcgcaaaagc tattgcagat ttgattgaaa agcctgttat
     9661 tattgctcca acaattgcat cgaccgacgc acctgtatct gctttatctg ttatttatac
     9721 agatgaaggt gcatttgatc attatctatt ttattctaaa aatccagatt tagttttggt
     9781 tgatacaaaa gttatttcac aagcccctaa gcgtttatta gcgtctggta ttgcagatgg
     9841 tttagcaact tgggttgagg cgcgtgcggt tatgcaggca aatggaaaaa ctatgttggg
     9901 acaacagcaa acattggctg gagttgcaat tgcgaagaaa tgtgaagaaa cgctgtttgc
     9961 agatggttta caggctatgg cagcttgtga agctaaagtg gtgacaccag cattagaaaa
    10021 tattgttgaa gctaatactt tattgagtgg tctaggtttt gaaagtggag gattagctgc
    10081 ggcgcatgca attcataatg gttttactgc attgacaggt gacattcatc atttaacaca
    10141 tggtgaaaaa gtagcttatg gaactttagt acaactatta ttggaaaata gacctaaaga
    10201 agaacttgat aagtatattg agttttacaa aaaaattggt atgccaacaa ctctaaaaga
    10261 aatgcatttg gatcaagttg gatatgatga tttaataaaa gttggtaaac aagcaactat
    10321 ggagggtgag acaattcatc agatgccgtt taagatttcg ccctcagatg ttgctcaagc
    10381 tattatcgct gtagatgcct atgtaaattc aaaataaaca ataaggacta ctgttttcca
    10441 aatggtagtc ttttattgat ccctgtattg aattctatag cagattgaaa taggatgaga
    10501 acaaatcgat tgggaaagta aaattaattt ctataaatgt tttagcaatt gtttcgtact
    10561 atttcagatt cagtctacta tacgttcttc ataaatcaaa aagcgacata ggtagtcggc
    10621 tatttattgt gaatacatta attagcattc cagttttatc ttcggtctaa aataagtatt
    10681 ttgtgctata cgagataagc ttcttgactt actccttgat ttactgcata acaatgggat
    10741 aaaaagtggg agatagagca attcatagtc atcaaaatta atgagataca gtatacagtt
    10801 tttcctttaa acacatttca aattccctca aaaatggtat aatagtaaca tcacaaaatt
    10861 ggagagagac catgagtttt tacaatcata aagaaattga gcctaagtgg cagggctact
    10921 gggcagaaca tcatacattt aagacaggaa cagatgcatc aaaacctaag ttttatgcgc
    10981 ttgatatgtt cccttatccg tctggagctg gtctgcacgt aggacaccca gaaggttata
    11041 ctgcaaccga tatcctcagt cgttacaaac gtgcgcaagg ctacaatgtc cttcacccaa
    11101 tgggttggga tgcttttggt ttgcctgcag agcaatacgc tatggatact ggtaatgacc
    11161 cagcagaatt tacagcggaa aacattgcca acttcaaacg tcaaatcaat gcgcttggat
    11221 tttcttatga ctgggatcgt gaagtcaaca caacagatcc aaactactac aagtggactc
    11281 aatggatttt caccaagctt tacgaaaaag gcttggccta tgaagctgaa gtgccagtaa
    11341 actgggttga ggaattggga actgccattg ccaatgaaga agtgcttcct gacggaactt
    11401 ctgaacgtgg aggctatcca gttgtccgca aaccaatgcg ccaatggatg ctcaaaatca
    11461 cggcttacgc agagcgcttg ctcaatgact tagatgaact agattggtca gagtctatca
    11521 aggatatgca acgcaactgg attggtaaat caactggtgc caatgtaact ttcaaagtaa
    11581 aaggaacaga caaggaattt acagtcttta ctactcgtcc ggacacactt ttcggtgcga
    11641 ctttcactgt tttggctcct gaacatgaat tagtagatgc catcacaagc acagagcaag
    11701 cagaagcagt agcagactac aaacaccaag ccagccttaa gtcagacttg gctcgtaccg
    11761 accttgccaa agaaaaaact ggtgtatgga ctggtgctta tgccatcaat cctgtcaatg
    11821 gtaaggaaat tccaatctgg attgcggatt acgttcttgc tagctatggt acaggtgcgg
    11881 taatggctgt gcctgcccac gatcaacgtg actgggaatt tgccaaacaa tttgaccttc
    11941 caatcgtcga agtacttgaa ggtggaaatg ttgaagaagc tgcctacaca gaagatggcc
    12001 ttcacgttaa ttcagacttc ctagatggac tcaacaaaga agacgctatt gccaagattg
    12061 tggcttgctt ggaagaaaaa ggctgtggac aagagaaggt aacctaccgt ctccgcgact
    12121 ggctctttag ccgtcaacgt tactggggtg agccaatccc aatcattcat tgggaagatg
    12181 gaacttcaac agctgtccct gaaagtgaat tgccacttgt cttgcctgta accaaggata
    12241 tccgtccctc aggtactggt gaaagtccac tagctaactt gacagactgg cttgaagtga
    12301 ctcgtgaaga tggtgtcaaa ggtcgtcgtg aaacaaatac tatgccacaa tgggcaggtt
    12361 caagctggta ctacctccgc tatattgacc cacacaatac tgagaaattg gctgacgagg
    12421 atctgctcaa acaatggttg ccagtagata tctacgtggg tggtgcggaa catgctgtac
    12481 ttcacttgct ttacgctcgt ttctggcata aattcctcta tgacctcggt gttgttccaa
    12541 ctaaggaacc attccaaaaa ctctttaacc aagggatgat tttgggaaca agctatcgtg
    12601 accaccgtgg ggctcttgtg gcgactgaca aggttgaaaa acgtgatggt tctttcttcc
    12661 atgtggaaac aggggaagag ttggagcaag cgccagccaa gatgtctaaa tcgctcaaga
    12721 acgttgtcaa cccagacgat gtggtggaac aatacggtgc cgataccctt cgtgtttatg
    12781 aaatgttcat gggcccactt gatgcttcaa tcgcttggtc agaagaaggt ctggaaggaa
    12841 gtcgtaaatt ccttgaccgt gtttatcgtt tgattacaag taaggaaatt attgcggaaa
    12901 acaatggtgc tcttgacaag gtttataacg aaaccgttaa agctgtcaca gagcaaatcg
    12961 aatcattgaa attcaacaca gctattgccc aacttatggt ctttgtcaac gccgctaaca
    13021 aggaagacaa gctctatgtg gactatgcca aaggatttat ccaattgatt gcgccatttg
    13081 cacctcactt ggcagaagaa ctttggcaaa ctgttgcagc aacaggtgaa tcaatctctt
    13141 acgtagcttg gccaacatgg gatgaaagta aattggttga agacgaaatc gaaatcgtcg
    13201 tccaaatcaa aggtaaagtc cgtgccaaac tcatggttgc taaagacctg tcacgtgaag
    13261 aattgcaaga aattgctctt gctgatgaaa aagtcaaggt agaaattgac ggtaaggaaa
    13321 tcgtgaaagt gattagtgta ccaaataaat tggttaatat tgttgtaaaa taaccaattt
    13381 atttgctgat ttccaacctc aaacagtttc ccaaactgtt tgagccaact aaactcgtta
    13441 atatcgttgt gaaataaggt aggaatcctt cagagtagaa tctggaggat tttttgaatc
    13501 ttcttatgaa agtatggtat actatgggca actataaagt ttgaaaagtg aaaaaaggag
    13561 aaagctatgc cagtaaatga atatggtcaa atgattgggg agtcaatgga aggttataca
    13621 ccaggtgaac tgccttctat tgatttctta gaagggcgtt atgctaggat agaggctctt
    13681 tcagtggaaa agcatgcgga ggatttgcta gctgtttatg ggcctgatac gcctcaggag
    13741 atgtggacct acctttttca ggagccagta gcagatatgg gggagctggt tagcctttta
    13801 catcagatgt tggctcgtaa ggaccgtttt tactatgcaa tcatagacaa ggcgactggt
    13861 aaggctttgg gatctttttc tctcatgcgc attgaccaga ataaccgagt aatagaagtc
    13921 ggagcagtca ctttttctcc agagctaagg ggtacacgga taggaactga agctcagtat
    13981 ctcctagctc gctatgtttt tgaggagcta aactatcgtc gctacgagtg gaaatgcgat
    14041 gccttaaatc tgccatccag acgagctgcg gagcgtttga gctttattta tgaaggaacc
    14101 ttccgtcagg cagtggttta taagggacgt acgagagata cggattggtt gtctatgatt
    14161 gataaggact ggcctcaagt caaagatcgt ttggaaacat ggttgcgtcc tgaaaatttt
    14221 gataaaaatg gacagcagta caagagcttg agagaacttt aagaggtgtt gagatgatta
    14281 ctattaaaaa gcaagaaatt gtcaagctag aggatgtttt acatctctat caggctgtcg
    14341 gttggacaaa ttatacccat caaccagaga tgctggagca ggccttatct cattcattag
    14401 taatttatct ggcacttgat ggtgatgctg tggtgggctt gattcgtttg gttggagatg
    14461 gattctcatc agtattggtt caggatttaa tcgttttacc aatctatcag cgtcaaggaa
    14521 ttggtagtgc cttgatgaaa gaggctttag aggattacaa agatgcctat caagtccagc
    14581 tggtgacaga agagacagaa agaaccttgg gcttctatcg ttctatgggc tttgaaatct
    14641 tatccaccta taattgtata gggatgactt ggatgaatcg taaaaaataa caaaacttgt
    14701 tttttcttaa gcaaaattta aggatggtct agtattatat agtcattaaa taaagacctc
    14761 ctaactttat ttaataaaat cctaaacttt tttcatcaca atctcctaat gaagccaccc
    14821 aatcaggtgg cttttttgcg tggtgaggtg ctggtgatag aaattttttg caaaatagta
    14881 aaattttaga aaagttaagc tagttttaag tttcgtcttg tatcatgtag ttattaaata
    14941 aagacctcct aactttattt aataa
//
LOCUS       DAVBDG010000047        14115 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBDG010000047 DAVBDG010000000
VERSION     DAVBDG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.048"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(30..1880)
                     /locus_tag="VQ526_001513"
     CDS             complement(30..1880)
                     /locus_tag="VQ526_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2555220.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSAFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(1948..3087)
                     /locus_tag="VQ526_001514"
     CDS             complement(1948..3087)
                     /locus_tag="VQ526_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2555221.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            3145..4311
                     /locus_tag="VQ526_001515"
     CDS             3145..4311
                     /locus_tag="VQ526_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2555222.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            4450..4776
                     /locus_tag="VQ526_001516"
     CDS             4450..4776
                     /locus_tag="VQ526_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2555223.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            4763..5356
                     /locus_tag="VQ526_001517"
     CDS             4763..5356
                     /locus_tag="VQ526_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2555224.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKRKRGNQHA"
     gene            5349..6260
                     /locus_tag="VQ526_001518"
     CDS             5349..6260
                     /locus_tag="VQ526_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2555225.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPIY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILNQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6323..7387
                     /locus_tag="VQ526_001519"
     CDS             6323..7387
                     /locus_tag="VQ526_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555226.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVPQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7564..8424)
                     /locus_tag="VQ526_001520"
     CDS             complement(7564..8424)
                     /locus_tag="VQ526_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2555227.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPLSFYHQLDANQIEVQPILD
                     KDFKALAQEE"
     gene            complement(8546..9532)
                     /locus_tag="VQ526_001521"
     CDS             complement(8546..9532)
                     /locus_tag="VQ526_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2555228.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9804..11279)
                     /locus_tag="VQ526_001522"
     CDS             complement(9804..11279)
                     /locus_tag="VQ526_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2555229.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11363..12286)
                     /locus_tag="VQ526_001523"
     CDS             complement(11363..12286)
                     /locus_tag="VQ526_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2555230.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12300..13229)
                     /locus_tag="VQ526_001524"
     CDS             complement(12300..13229)
                     /locus_tag="VQ526_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2555231.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGTLWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLSIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13548..13768)
                     /locus_tag="VQ526_001525"
                     /pseudo
     CDS             complement(13548..13768)
                     /locus_tag="VQ526_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13722..13982)
                     /locus_tag="VQ526_001526"
     CDS             complement(13722..13982)
                     /locus_tag="VQ526_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555232.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         4214 a         3059 c         2584 g         4258 t
ORIGIN      
        1 tttaacttga taatatgcgt ttttcttttt tattcaatgt gggttgtttg attagcaaag
       61 gcgataatag cttgcttcaa ctcatctcca ctgagagtgc ggaactctcc aatcttttga
      121 ttgatggatt ctaaaggcat gacattaacc ttaccaacga aaatcttatc cataacttga
      181 ttatcaacga gttcggttga taccaagagt tcttcgtaga gtttttcacc tgggcggatt
      241 ccaacttcaa cgattggaat ttcactttca gtgtggccac ttagaagcac catcttcttg
      301 gccaagtcat aaatcttgac tggtttgccc atatcaagga taaagacttc cccatctttg
      361 gcataagcac cagcatggat aaccagacgg ctagcttctg gaatggtcat aaagtaacgg
      421 gtcatgcgga agtctgtcac cgttacaggc ccaccttcag caatctgacg ttcaaagact
      481 ggaatgacac taccacggct accaagaaca ttcccaaaac gaactgcaca gtaggttgat
      541 tggctacgtt ggttaaagcc agtgacaatc aactccgcca cgcgcttggt tgctcccata
      601 acatttggtg gattgactgc cttatctgtc gaaatcataa ccatcttagg caccttggct
      661 tcatcaacgg ctctagcaac attgtaagtt ccacggatat tgtttttgaa ggcttctttt
      721 ggattgcgct ccatcatagg aacatgcttg tgggctgccg catgataaac aatagcaggt
      781 ttgtactgct caaagacttg caacaaacga tcatagtctt gaatgtccgc aatcacaggt
      841 acataatcaa tcccttggaa cttacgaatc aattcatgat aaacaaggta gattgagttt
      901 tccccatgac cgagcaagac aatgcgttca ggattgaagc gactaacttg acgacagatt
      961 tcagaaccga ttgaacctcc agctcctgtg actaagatgg tcttacctgt cagttctgca
     1021 cccagacgcg attcgtcaag acggatttcc tgacgaccca aaaggtccgt aatatcaatt
     1081 ttttggaagc cagtacctgc ttggtgaagg ccctgaacaa cagtttcaac cttaggcatc
     1141 ttgtaacatt tgacacccag cttattacac atctgcaaga tacgctcata ttctgacgga
     1201 tccagcgacg gaatcgcaac gatgacacgc tcgatttgat ggcgtttggc taattcaggc
     1261 agattgtcat aagagcccaa aacaggaata ccaccaagtt tttgaccctt tttcttagaa
     1321 tccttatcca aaataccgac cagttctaat tcactggttg gatgttggta actatccata
     1381 aagagagccc caccatcacc ggcaccaatc aagaaggtcc gacggtgttc tccatcacca
     1441 ctaccttttt tgcgtctgga gtagattaac tgccaagtaa tccgtggcaa taaaatcaag
     1501 aaggcactca acaagataaa gagaatgata aaacggatgg agaagagtgg caagaaggca
     1561 taacagatac tatatgacaa gacactgcta gcagtcacac caaaaaagat tttcatgaaa
     1621 tccgtaatct tgctgtaacg actaatactc gcgctcaagc cccaaaaatc aaccatcaat
     1681 tgatagaaca ggaaggccaa actcgtatag ataatgtagt caacaggtgc tggattaatc
     1741 agcccataaa ataaaatata agatacaatg atggaaacca ccatactgaa aatatcaaat
     1801 attccccaga aaacctgctt ttgttgttta tttaaaattt ccaccagatc aatcacataa
     1861 tctgtgagtt ttttattcat agaaatttac tcctccttaa aatagttcta ctatattgtt
     1921 attatagcac attttaattt atttggctta tttttccaaa taaattactg ttcccttgta
     1981 ctccttcttc catttaaatc ctctaattaa acctacaatc gtcccaaggc cataagcaaa
     2041 gtgaatggaa aataaaagaa agggcatcac aattagaaat ccatttttat gttttaataa
     2101 agtcagcaaa gtgagtaatg acagtaatga taaaagtaga aaataggcac ctaatagtaa
     2161 agttatgaat acgaatgtga tcggtaacaa tgctagacta aacacaagac tcaaaacaaa
     2221 taaacaagga acataatgaa ataatgataa acacttaaac tgaacatgac ttgtcaagcc
     2281 aatccacaaa ccatttgaat acttttgatg cagcattttc ttgaatgttg gtcgaatata
     2341 ctgataagat agaatacttg ggctatagcg gattttataa ccatgttctc gaattctata
     2401 atgaatatca ttatcttcag ttcggccaag ttgctcattt actaaaccaa ccttctggaa
     2461 aacctctcgt ttatacatcc catgaaaaat agaagaaaca tatctatcct cggaactatt
     2521 tcgataattg gcaatgctac tgccaaacat attttcctca acaagatgca aggtctctgc
     2581 ccattttcct tttcctttga caatcgtcgg tctaggccct ccacagacaa attcaccttg
     2641 ttgaataaca gccacattgt tcattacaaa actctcagta acttttgaat gagcatcaat
     2701 ttttaaaata aggcccccta cagaatgctt aactcccagg ttaaaaccac tagcttgatt
     2761 tttcttagga ttgttataca atctaattga gttaaactct gcatcttctt ttataaattg
     2821 ctgaatgata gctgtggtcc catctgtgga catagcattt ataaatagaa tttcaatatt
     2881 ctctttagga taggtttgat tttttaagtc ttcaattaga ccaggcagat atttttcttc
     2941 attgtatgca ctgattacaa tggatacgag aagactttca acattttgag tcactactac
     3001 tctcctgaat tttcccttcc accattctat cataattttc aagacttttc catttttatc
     3061 ttgaaagcca gaaaccttac ttgacattat agtgaaaaag ccttaaaata agctgttatt
     3121 aaaatcagct agaagagata ttatatgaaa aagcaatcac tcttttttgt tccaggtatt
     3181 atcctgattg gtgtttcctt gcgaactcct tttactgttt tacccattat tttgggaaat
     3241 atttcgcaag gtctggaggt agaagttagt tcgcttggtg tcttgaccag cctgcctctc
     3301 cttatgttta ccctcttttc accattttct acccgactgg ctcagaaaat cggcttggag
     3361 catctcttca cctacagcct cttcttcttg accatcggct cacttattcg actaatcaat
     3421 ctgcccctgc tctatctagg aaccttgatg gttggggcaa gtgtcgcagt catcaatgtc
     3481 ctgcttccta gtcttatcca agccaatcaa ccaaagaaaa ttggttttct gaccacctta
     3541 tatgtaacgt ctatggggat tgcaacggct ctggcttcct atctagctgt gcccattaca
     3601 caagccagtt cttggaaagg acttatcctc ctcctcacgt tactctgtct agcaactttt
     3661 ttggtctggc tcccaaatca ccgctataat catagactag ctccacaaac caaacaaaaa
     3721 agtcaaataa aggtcatgcg taataaacag gtttgggcaa ttattatctt ttcaggtttt
     3781 caatccttga tcttttacac cgccatgacc tggttaccta ccatgtctat ccatgcaggt
     3841 ctatccagtc acgaagctgg cttgctgact tctatcttat ctctgattag cattcctttt
     3901 tcaatgacca tcccaagcct gacaaccagt ttatctactc gcaaccgtca gctcatgctc
     3961 actctggttt cactagctgg tgtggtcggc atttccatgc tctttttccc aatcaataat
     4021 ttcatttact ggcttgccat ccatctcctc atcggaaccg caaccagcgc cctcttccct
     4081 tatctcatgg tcaacttttc actcaagaca agcgcccctg aaaagacagc ccaattgtcc
     4141 ggcctatctc aaacaggagg ctatatccta gcagcctttg ggccaaccct ctttggttac
     4201 agttttgacc tattccactc ttgggtacca gctgtagctg ccctcttgct catcgatatc
     4261 ctgatgactg tggccctctt tacagtggac agagcggata agatccttta aacttctagt
     4321 tttagctaga agttttttat atataaaaat tttacacatt tctcttgaca gggctttctt
     4381 ttagatgtac aatgtatgtg tagaaaaatt atatataaaa atcttacaca ttagaaaagg
     4441 aggtttccca tgtactttcc aacatcctct gccttgattg aatttctcat cttggccgta
     4501 ctggagcagg gtgattctta tggttatgag attagccaaa ccattaagct gatcgctaat
     4561 atcaaagaat ccacactcta tcccattctc aaaaaattgg aaggcaatag ctttctgaca
     4621 acctattcta gagagttcca aggtcgcatg cgcaaatact actccttgac aaacggtggt
     4681 atagagcagc tcttgaccct aaaagatgaa tgggcactct atacagacac catcaatggc
     4741 atcatagaag ggagtatccg ccatgacaag aactgaatac ctgactcagc tagaactcta
     4801 tctcaagaaa ctacctgaag ctgaccgtat cgaagccatg gactatttca gagagctctt
     4861 tgacgatgct ggagtcgaag gagaagaaga actcatcgct agtttgggaa ctcccaaaga
     4921 agcggcccac gaagttctat ccaatcttct cgataaaaaa atcaatgaag cacccgctca
     4981 aaaaaataac cgacaaattt tacatatcgc cttgttagcc ctccttgcag cacctatcgg
     5041 cattcctctg ggaatcgcca tcctcgtgac cctgttcgca atccttgtag ccgctttgac
     5101 tgtcattctg gctttctttg cagtttccat actgggtatc atcggcggat tcctattttt
     5161 agttgaaagt ttcactgtcc tcgcccaagc caaatcagcc tttatcttga tttttggttc
     5221 tggtttactg gctatcggtg cttcttcgct agttttacta ggcatttcct atgtagctcg
     5281 cttcttcggt ctactcattg ttcgcctggt acaatttgtt cttaaaaaaa gaaagagagg
     5341 taaccagcat gcgtaaatgg acaaaaggat ttctcatctt tggtgtggtg actaccgtta
     5401 tcggctttat ccttcttttt gtaggtatcc aatctgacgg gattaaaagc ctactttcca
     5461 tgtccaaaga acctatctat gatagccgta cggaaaagct aacctttggc aaggaagtcg
     5521 aaaacctaga aattactctc caccaacacg cgctcaccat cacagactct ttcgatgatc
     5581 aaatccacat ttcttaccat ccatctcttt ctgctcacca tgatcttatc accaatcaga
     5641 acgatagaac tctgagtctc actgataaga aactgtctga aactccgttt ctctcttctg
     5701 gaattggtgg gattcttcat atcgcaagta gctactctag tcgttttgaa gaagttattc
     5761 tccagctacc aaaagggaga actctaaaag ggatcaacat ctcagccaat cgcggacaaa
     5821 ccaccatcat aaatgctagc cttgaaaatg cgaccctcaa tacaaacagc tatatcctcc
     5881 gaattgaagg aagtcgtatc aaaaacagta aactcacaac gcccaatatc gttaatatct
     5941 ttgatacagt tcttacagat agtcagctag agtcaacaga gaatcacttc cacgctgaaa
     6001 atatccaagt ccatggcaag gttgaactga ctgccaaaga ttatctcaga atcatcctaa
     6061 accagaaaga aagccaacga attaactggg acatctcaag caactatggt tctatcttcc
     6121 aattcacaag agaaaagcct gaatcaagag gtacggaatt aagcaaccct tacaaaactg
     6181 aaaaaaccga tgtcaaggat caactcattg cgagatctga tgataatatt gatctaatat
     6241 ccacaccaag cagacgttga caaaaacgct tacatctgct actatggaag catatttatc
     6301 taactaaaaa ggaggttcta ccatgaaaca agaatggttt gaaagtaatg attttgtaaa
     6361 aacaacaagc aagaacaagc ctgaagagca agctcaagag gttgcagaca aggctgaaga
     6421 aacgatagcc gatctcgata caccaattga aaaaaatact cagttagagg aggaagtccc
     6481 tcaagctgaa gtcgaattgg aaagccagca agaagagaaa attgaagctc ctgaagacag
     6541 tgaagcgaga acagaaatag aagaaaagaa ggcatctaat tctactgaag aagagccaga
     6601 cctttctaaa gaaacagaaa aagtcactat agctgaagag agccaagaag ctcttcctca
     6661 gcaaaaagca accacgaaag agccacttct tatcagtaaa tctttagaaa gtccttatat
     6721 ccccgaccaa gctccaaaat ctagggataa atggaaagag caagtgcttg atttttggtc
     6781 ttggctagtg gaagcgatca aatctcctac aagtaagttg gaaacaagta tcacacacag
     6841 ttacacagcc tttctcttgc tcattctgtt ttctgcatct tcctttttct ttagtatcta
     6901 tcacatcaaa catgcttact atggacatat agcaagcatt aacagtcgct tccctgagca
     6961 gctagctcct ttaactcttt tttctatcat ctctatccta gtagcgacaa cactcttctt
     7021 cttttcattc ctcttgggta gtttcgttgt gagacgattt atccaccagg aaaaggactg
     7081 gacgctagac aaggttctcc aacaatatag tcaactctta gcaattccaa tcttcctcac
     7141 tgctattgct agtttctttg ctttctttga tagcctacga tttacagccc tcttgtgtgt
     7201 gattagcatt ggaatcattc tgcttgccag tctccatatc attacaagac caagtcaagc
     7261 aagtgaaacc gactccttct atcaattatt cttgtctgtc cttgtgaacg gagttattat
     7321 cctcctcttc tttgtagctg aagtggcact gattggagat tatcttcgta tcttggcctt
     7381 tctttaaact tcatactctt cgaaaatcaa attcaaacca cgtcagcttc gccttaccgt
     7441 actcaagtac agcctgcggc tagcttccta gtttgctctt tgattttcat tgagtagcaa
     7501 tcctgtcttt catggcagga tttttctatt tcaaaaaagc aagtcgattg acctgcttct
     7561 gctttactct tcttgtgcta atgctttaaa atctttgtct aatatgggtt gcacttctat
     7621 ttgattggcg tctagttggt ggtaaaaaga taggggtaat acctctaaga attttgcata
     7681 gtccaagcgg gcaatggctt catagtcttc tggtttagag ccatctcctg tgatttgaac
     7741 caagtcaatc tcccactgca cttccttatc caccaattgc tcaatatagg ctgcaggaac
     7801 aaacggttct ctcggtaaaa ctgtcttgac tccttgagct agatggtaaa cgccatgcac
     7861 ttttccttct ccatcctgat agaaggaaac tctcgcaaag taggcatctg tctcttgaac
     7921 cgcacgcaca cgcgcttcaa actgagccaa gccatctgcc tctaaaaagc ttttcaagtt
     7981 ctcacgacta aagaaaatag gattaaaaat tccttggcaa atcgtaaaac gagctacagg
     8041 gtctgctaga taggcttgaa tacttgcttg gaaataagct tcaaaatcaa aaccatctag
     8101 cccttcaata tcagtccctg tataagcaag caaagcatct aaaaatgact tgataatctg
     8161 ccagtctgtc tttgttagta ggtcaggaag atcgacacgg taagcctttt gaccatcagc
     8221 ataactgacc ttaaaaagaa attgtgattg ccccactatc gcacactcaa tatactcgag
     8281 acgattaaga ggctgacgga gatagactac atcatagcta tgtgactcca aaccgtctag
     8341 caaggccaag atcgacttgg cattcaaaat ctcttgttct cctaaaatgc tctgtttatt
     8401 tggaataaaa aatgtttttg ccataactgg actcctttga aatcgtttac atacagtata
     8461 ccttattttt ctgaaagata cagaaacaaa ctttagattt ttgagtacaa agcatagaaa
     8521 aacagcccct gaggactgtt tgatcttata cagtagctgc ttgatccaag ctttcaccga
     8581 tagcggctag gcgctcaatc acttcagctt gtgtcaattc attttttgaa acatagcggt
     8641 tacgtgggtg aacacggcac tcgtgtgagc atccacgaag gtacttgtct tcattttctt
     8701 ctgatgtcaa gatacgacgg ttacagaatg gatttccaca gttgacataa cgttcacatg
     8761 gtgttccatc aaaccagtct ttccctacga tagttgggtt gacatggttg acatcaacgg
     8821 caatacgctc gtcaaagacg tacattttcc catcccaaag ctcaccttga acttctgggt
     8881 ctttaccgta agttgcgatt cctccgtgca attggccgac atctttgtaa ccttcacgaa
     8941 ccatccagcc tgagaatttc tcacagcgaa cgccacctgt acagtaaacc acgacacgct
     9001 tgtccatgaa tttttccttg ttatcacgga cccattgtgg taactcacgg aagttgcgaa
     9061 tatctgggcg aatagcccca cggaaatgtc ctaggtcata ctcataatca ttacgtgtgt
     9121 caaggacaac ggtatcttta tcaagaagcg cttctttgaa ctcttttgga gacaagtaag
     9181 cacctgttgt ttcaagtggg ttgatgtcat tgtcaaagtc gttgtcttcc aaaccaaggt
     9241 ggacaatttc tttcttgtag cgaacaaaca tcttcttgaa ggcttgttca ctttcttcgt
     9301 caatcttgaa ccagagttct tccattcctg gaaggctgtg aacgtagtcc atgtattttt
     9361 gagttgtttc atagtcacct gaaactgttc cgttaattcc ctcgtcagcg actaggatac
     9421 ggcctttaag accgattgat ttacagaaag ccaagtggtc tgcagcaaat tgctctgcat
     9481 tttcaattgg agtataaagg tagtaaagta agacacgaat atcttttgcc ataagatttg
     9541 ttctcttttc tattcttaaa ttttctaaat tttttattta tctacactag tatacctgct
     9601 tgagaaaacg aatgcaattt atttgactgg aaattgtaga gggttctcga aatttcctga
     9661 atggttaaaa taaggacaag agaaaacatg gatatctata tccttgtgcc aaaaaaacca
     9721 ctgtctcccc cagaccaacc tgaggaaagc agtgattctt attttaggag ttaggaatga
     9781 atacacgaaa tcaatttagc tgattatttt ttgtttttca agaattcatc gtattgtttt
     9841 tgcatttcgt tcaatacttt ttcgtaggca ccttcagatt tcaatttttc catcaattct
     9901 ggaatcgctt tatctgggtc tacagtacca gtgttgatag ctgtatcaaa ttgttgcatt
     9961 gtgttagcaa tagctgagat ttcagatttc acattgtcag tattgaagat aaatccaagc
    10021 gctggagatt ctttagcttc tgccaattct ttcttagaat tttcgatttg ttggtctgta
    10081 acgttttcgt tgatgtaaag gatccagttg ttaccagtgt tccatccacc catgtgagtg
    10141 tttcctttgt agccatcaag aacgcgaaca cggttttctt taccttcaat tttttcccag
    10201 ttcttgcctt ctggaccgta aacaagaccg ttcaagagtt ctggattcgt attcaagagg
    10261 ttcaagattt ccattgattt ttctttgttc ttagagttgt ttgagatgac aaagttagca
    10321 acttgtgttg tttggttttt cttgatgaag ttagtaattg gtttgatttg gatatctttg
    10381 ttggcaacac gtgaaagcaa gctgttaccg tagtcagctg gtcctactgt ttcttcacga
    10441 acgaaccaag tatcttgttg aaggtcaaag gaagtatcgc ttgttgcgac gtcttttgga
    10501 atgtagccag cttcatagaa tttgtgaaga gtcttcaagt gttctttgaa acgaggtact
    10561 tcgtaacggt ttacaacttt agtagtatcg ccttcaaggt caataacgaa tggaagaccg
    10621 tttgctactg ggtagtcaaa gttatcagat gggatgaaaa ctttaccaat agcaaatggt
    10681 actacgtctg gagctttttc tttgatttgt ttcaagactg gctcaagagt ttcgtaagaa
    10741 gtaacacctg aaatatcgat accatatttg gcaaggagag ttccgttgaa ggcaaagttt
    10801 tgagatgatg ctacgttggc tgcaactggt acagcgtaaa tcttaccatt tacagtatta
    10861 cccttgatat aagctggatc aagtgccttg taaaggtctt taccttcttt tttgtacaat
    10921 tctgtcaagt cagcataagc acctttttga gcatttacaa tatagttatc tgcaaaggcg
    10981 atatcatagt tttcaccaga tgatgtgata actgacattt tcttaccata gtcaccccag
    11041 ccaaggtatt ggatatccaa tttagcacca actttttctt cgatgatttt gttggcattt
    11101 gctaacaatt catccaagtt atctggtttg tcaccgattt ggtacatttt gataacaggt
    11161 ttgtcacctg aatcagcagc ttttttgctg ttacctgtca agttgccaca agcagcaaga
    11221 cctgcagcca aagcaactac actagcagat gcaaaagcat attttttcca gtttttcatg
    11281 ataaaaactc ctttttttat ttttaaactt ataaacaatg taatgatctt aacttcacgc
    11341 aagggaagtt tggaaacaag tgctattctt tcacaccacc gatagtcaaa ccttttacaa
    11401 agtagcgttg gaagaatgga tacaaaatcg cgattggaag ggttgctacc acaaccatgg
    11461 ccatacgacc tgtttctttc ggtagagcaa ctcccagttg accagtcaag ccgaccgctt
    11521 tggcaatgta gtccatattt tgttggattt gcatgagcaa atattgcaat ggatacaagt
    11581 tatcactctt gatgtaaaga agggcgttga accagtcatt ccagaaacca agagctgtca
    11641 aaagcgtgat ggttgcgata cctggtagtg acaatggcaa acagatttgg aagaaaatcc
    11701 gggcctcact ggcaccatcg atacgagccg attcgagaat ggcttctgga atggtcttct
    11761 tgaagaagga acgcatcaag atgatattga atggtgagag aagcattgga acaatcaagg
    11821 cccaaacagt gtcaccaagc tgaagtacac gggtcaccat gatataacct ggtaccaaac
    11881 cagcgttgaa caacatactg agaaggacga agatggtaaa aaatctgcga tacttaaagg
    11941 ttgtccgtga aatagcgtag gcataggttg ttgtgataaa gacatttgtc aatgtcccaa
    12001 ctacggttac aaagacagag atgaagaggg cttgtaggat tttatcctta aactgtgcca
    12061 aaaactcaaa accgtctaag ccaaattggg atgggaagaa gctatagccg tattggagga
    12121 ggcttttctc gtctgtcact gaaataatga taacgaatac aaaaggtagg atacaagaga
    12181 gggcaatcaa acccgaaatg atactgaaga agatatctgc tttcttactg aaggagtgaa
    12241 tgccgacatt atcaattttt tcttttttaa ttttcttttt tgccatattc tcctcctttc
    12301 tagaacaaag ctgagtttgg atcgactcgt cttgcaagca agtttgatag gataaccaga
    12361 atcaaaccaa caacggattg gtaaagaccg gctgctgcag ccataccgat atctgctgtc
    12421 tgagtcaaac cattaaagac atatacgtcc aaaacgttgg ttacattgta aagctgacca
    12481 gcattgtgtg ggatttgata gaagagacca aagtctgcgc ggaagatatt tccgactgca
    12541 aggatggtca atacagttac aagcggagtc aactgaggaa tggttacgtt gcgaatacgt
    12601 tgccacttgc tagctccgtc cactgtcgct gcttcgtagt aggttggatc aattcccatg
    12661 atcgtcgcat agtacatgac actgctatat ccaaagcctt tccaaatacc taggaaaagt
    12721 aggagatagg gccagatgct caggtcagcg taaaaattga cttctttgag accaagactt
    12781 tccaatagat gattgaacac ccctttatca atatttagga aggcatctgt aaagaaactg
    12841 atgataaccc aagacaagaa gtaagggaac aacatagaag tttggaaaat cttcaccatt
    12901 ctcttagaac ggagctcgct gaggataatg gcaatcccta cagatacaac taaacctaga
    12961 aagataaagc caagattgta gaggacagta tttcgtgtga taataaaggc gtctcttgaa
    13021 ctaaataaga atctgaaatt atcgagtccg acccatttac tatttatgat actatctatg
    13081 aaaccattac tggtcatgtg gtagtctttg aaggcaacca cgttcccaaa tactggaatg
    13141 taaaagaata gaatcaacca gagtgtccct ggcaaaacca tcaagagaaa gatccagttg
    13201 tctctcaatg tttttgaaaa ctttttcata atttcctccc tttttatttt gatatccatc
    13261 taaaaattct tttttagact tttgataacg attacattat tagtatactc ctatttgcag
    13321 gttaggttaa actcctaatt atagaaaaaa ctccacaaat tatgtagcag atttaaaact
    13381 ttatcaccac tatcaaacaa atgtcctaaa tcaattgttt attttatctc tattagccca
    13441 gtgatggcgt cactctgtta taagcatcca acaacggggt atactgaaaa atctccagac
    13501 tagggaactc agagatagtt cctaatctgg agatttttaa tatgttatta ggcgtttgct
    13561 ttcaacttag caataacctc tttaagatta tcaatcacct ctgcttcagt atgctcagag
    13621 ctttttcatc tgccaagaac aaaactgctt tttgaagttc tttttgagag ttttcaagga
    13681 catccttatc tactgtttca aggtttgagt ctttaagaag tttacttaat tccttggcta
    13741 atttcttgag tttgatttgc agactcatct tctcctgctg tttctttgcc cgctgtttgt
    13801 cctccatcct tagttgctga ctggctttcc ttaatggact ctagggaagc aatggcatct
    13861 ttgactgttt gcaaaatatc acgtaaacct tgctctgtca aactatcatc tgcaaaagct
    13921 ttcttagcct ctgccaaaac cagacgtgct gaatctgtgg taggattcga tacacctgtc
    13981 aatgatctca aaagattttc taaggtttga gtctgcttac taatactaga ctaaaatcaa
    14041 aaagcattat ataacagtga tatgaaatca actaaagaag aaatccaaac tatcaaaaca
    14101 cttttaaaag actct
//
LOCUS       DAVBDG010000048        14010 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBDG010000048 DAVBDG010000000
VERSION     DAVBDG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.049"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            83..880
                     /locus_tag="VQ526_001527"
     CDS             83..880
                     /locus_tag="VQ526_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2555233.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGFLMGLPYAFLNKNEDTYVD"
     gene            1028..2287
                     /locus_tag="VQ526_001528"
                     /pseudo
     CDS             1028..2287
                     /locus_tag="VQ526_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2383..3657
                     /locus_tag="VQ526_001529"
     CDS             2383..3657
                     /locus_tag="VQ526_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2555234.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VQ526_001530"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VQ526_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2555235.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMAEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VQ526_001531"
     CDS             4786..5088
                     /locus_tag="VQ526_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2555236.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLLLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VQ526_001532"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VQ526_001532"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2555237.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VQ526_001533"
     CDS             5962..7302
                     /locus_tag="VQ526_001533"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2555238.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VQ526_001534"
     CDS             7280..7492
                     /locus_tag="VQ526_001534"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2555239.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VQ526_001535"
     CDS             7489..8364
                     /locus_tag="VQ526_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2555240.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQVPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VQ526_001536"
     CDS             8357..9172
                     /locus_tag="VQ526_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2555241.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VQ526_001537"
     CDS             9165..9596
                     /locus_tag="VQ526_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2555242.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VQ526_001538"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VQ526_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2555243.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VQ526_001539"
     CDS             11272..12000
                     /locus_tag="VQ526_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2555244.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VQ526_001540"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VQ526_001540"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2555245.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4141 a         2546 c         3350 g         3973 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ttcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat gatatggagg
     1021 tcaccttatg acttgtgatt ttaaatctga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatgttga taatttggag gaagtagaag cagttatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac tccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaata ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggatggctt cagggaaatt ccctcaattt gttgacgagg gttcaagctg
     1741 ccacaatttg agctatactc gtgatgtggg tgcagtagcc tttattatag ctgttcgagt
     1801 tcaattgctt tgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa accctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttatcat gcattggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtgacgagg aagaagcacg caaggccatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caatcactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaatc aaatccactt
     2221 gtctatcggt cttgaaaatg tagaagattt gattgaagac ttacgcttag ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg ttttgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaca gaagtctaga aagaggaatg taatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gaaagccagt atgctgaact tttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaggcagga actggccttg
     2521 gctgagcaaa aggctatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattgc agagtcaaat ccaaaacttt gatacagaaa aagaattggc caagaaagaa
     2641 gttgaacaga caagccatca ggccttattg gctaaggaca aggaagtaca ggccttggaa
     2701 aaccagttgg cgaccttgcg tttggagcat gaaaatcaat tgcaaaagac cctttctgac
     2761 ctagaaaaag aacgcaatca ggtcaaaaat cagctcctac tgcaagaaaa ggaaaatgag
     2821 ttgtctttgg cttctgttaa gcaaaactac gaagcccagc tcaaagcagc cagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa tgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat caacttaaaa aaagaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggccgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaattactc tgggttattc cacgacgact
     3901 gaggatgcta gtggggaagt gatcgcagaa acccctgttt tgtctccctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta cccagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac ttacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgct aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctggt gggaacttag gcccagaatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaaacggtc
     4921 atggccaatt tgagtcaagg gttgttactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggagcctcaa gatgtcttta
     5521 tcgttgaggg cattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgca ttattcgtcg tatcaagcgt gatatggagg
     5641 agcgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat gaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaagtat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtttatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggcctgttt caagaacgct tcaagcaagc tctgccccaa
     6361 ttttctaaga gaattggggt agtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagggg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggacgat
     6541 ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgaaacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattagctca tttgcaaaat
     6781 caggaaaaac ggatggtaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttgcca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttggagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagacccat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagatcaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca cttgtctctg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gtaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ttgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattggaag aaattgccca gcaggttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgataccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 tttccgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta aggttcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggta ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaga
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattgccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgagacttt tgcagacaag atttttagct gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgtctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct ctctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctca
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaatgaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagtatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacagtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag
    11221 aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tcttaaaacc
    11341 tgggatggtc agacccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtct
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcggcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatctttgt tcacgcaggt
    11701 attgatttga ccttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttaca
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gctttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacagga gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccat ggaaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggag
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatgggg aaacttatat tttccatttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aaatacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt tcttggactt
    13141 ctccatatgg atgttatcca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc gacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 atccttctca atggagataa ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataagaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtttt tatactcttc gaaaatctct
//
LOCUS       DAVBDG010000049        13335 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBDG010000049 DAVBDG010000000
VERSION     DAVBDG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.050"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..137)
                     /locus_tag="VQ526_001541"
     CDS             complement(<1..137)
                     /locus_tag="VQ526_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2555246.1"
                     /translation="MKLSYDDKVQIYELRKQGYSLEKLSNKFGINNSNIRYMIKLIDR
                     YG"
     gene            384..3063
                     /locus_tag="VQ526_001542"
                     /pseudo
     CDS             384..3063
                     /locus_tag="VQ526_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            3363..4202
                     /locus_tag="VQ526_001543"
     CDS             3363..4202
                     /locus_tag="VQ526_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2555247.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            4220..6058
                     /locus_tag="VQ526_001544"
     CDS             4220..6058
                     /locus_tag="VQ526_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2555248.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            6071..7486
                     /locus_tag="VQ526_001545"
     CDS             6071..7486
                     /locus_tag="VQ526_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2555249.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            8078..9124
                     /gene="pheS"
                     /locus_tag="VQ526_001546"
     CDS             8078..9124
                     /gene="pheS"
                     /locus_tag="VQ526_001546"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2555250.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            9124..9633
                     /locus_tag="VQ526_001547"
     CDS             9124..9633
                     /locus_tag="VQ526_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555251.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            9710..12112
                     /locus_tag="VQ526_001548"
     CDS             9710..12112
                     /locus_tag="VQ526_001548"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2555252.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPEARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(12180..13181)
                     /locus_tag="VQ526_001549"
     CDS             complement(12180..13181)
                     /locus_tag="VQ526_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2555253.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
BASE COUNT         4179 a         2131 c         2891 g         4134 t
ORIGIN      
        1 ccataacgat caatcaattt aatcatgtac ctaatattag aattgtttat cccaaattta
       61 tttgaaagct tctctaagct atatccttgt tttctaagtt catagatctg aactttatca
      121 tcataactta atttcataat aaaaacaccc caaaagttag attttttctg tctaactttt
      181 ggggtgtagt tcatgtacac ctgatatgat gcgttttttg aattttatag actgctcgtt
      241 taaactctat ttacttcgta cattccggag cgagatggac tattagtcgc atacaaaatg
      301 agtgctattc ggattttatt ttcgtgtaca atttcagcca ttcttgttat aatcagccta
      361 taggaatcaa ggaggtgatt gttatggcta tttttgtgtc tttggatgga attgtggtag
      421 aagtacttga tgtcttttct tcttttaatg gggatagtga gtttttcttg tgtaaacgtc
      481 ttaaagacaa gagtcagttt gtgatggaac gctctcggtt cgaggagatg ttccaacttc
      541 aaagtagtca cttgacgacg caagaaaaat tacaattgtt tacctccgtg tttgctggcc
      601 gttatgatgt ttatgctaag agttttatca atgatcaagg gaaaattcag tattttcctt
      661 cctatgatta tggttggaag cagttgccac ttgaaaaacg gagtttccag acattgacgg
      721 actccgtttt gaaatctcat tttcgtgggg agacagctat cggtatcttt cctatgcact
      781 tagatgatag ctgtcatttt ttggtactgg attttgatga aggagattgg aaagaagctg
      841 gtttaaccat tcgaagaata gccagggaac gccagatgga agcccattta gagatttctc
      901 gttcgggtca cggactccat atttggttct tctttgagga agcgattccg agtcgagagg
      961 ctcgcttgtt tggaaagaaa ctgatagaac tggcaatgca ggaaagtatg caactgtcct
     1021 ttgattcttt tgatcgcatg tttccaaatc aggatgtcct tcctaagggg gggatttgga
     1081 aatttgattg ccttgccttt tcaaggagaa gcttaccatc aagggcgaac ggtctttgtg
     1141 gatgaacagt ttcagcctta tgaagaccaa tggaggtatc tacaagaaat tcagagggtt
     1201 tcaactgcta aagtggcact gttaatccaa gaagagttag gcaagcaaga attggacaag
     1261 gagttgaaga tcgtcttatc caatatgatc caacttgaaa aatcgtctgt gacacccaag
     1321 acactgtttt tcttgaaaaa tatggcttca ttttctaatc ccgaatttta tttaaagcag
     1381 gctatgcgac agccaaccta tcaaattcct gagagaatgt atttatttgg agaatccgat
     1441 tattatttat ggctgccaag aggcttgtta tatccattgc aagataaatt taagcaggta
     1501 tctgtggaag ataggagaaa ggtacaaagg tctattagcg tggaatttaa gggagaactc
     1561 acttttgagc aagagttagc cctgtcagat atgacttcta aagaaaatgg tttacttcat
     1621 gcggagactg gttttgggaa gaccgtttta ggtgctgctc ttatctctga acggaaaaca
     1681 aaaacaatta ttctagtcca taataagcaa ctcttagacc aatggctaga tcgcttaaac
     1741 tgctttttga ctttcgaaga ggaggaggct atccgttata cggcatcagg tcgtgaaaag
     1801 gtaatcggct atgttggaca gtacggtggg actaagaaat ggctgagtaa actggttgat
     1861 gtcgttatga ttcaatctct atttaagttg gaaaatagtc aaagtctttt ggatgagtat
     1921 gagatgatga ttgtggatga gtgtcatcat gtctctgcct tgatgtttga aaaagttgtt
     1981 gctcagttta gagggaagta tctttacggt ttgacggcta cgcctgagcg taagaatggt
     2041 catgagccta ttgtttttca gagaattggt gagatactcc atactgctga taagagggaa
     2101 acggatttta aacggcaatt gcaattaaga ttcacttctt ttggtcattt ggagattgaa
     2161 aagaccaaag caagtaattt tatacagctc agtgattgga ttgctacaga ctcagcgagg
     2221 aatcagttga ttctcaagga tattctagcc caagtggcag aaggacggaa tatcttggtt
     2281 ttagttaatc gaattcaaca gatagatgtc tttgaaaagt tattgaaaga gaaagaggtt
     2341 gatggctgtt acattattag cggaaaaacc aaagtccgag aaagaacgag tttactggag
     2401 acgttagaac agttagataa agggtttgtt ttgttgtcta ctggaaaata cattggcgaa
     2461 ggttttgact tacctcagtt ggacacgctt atcttggcag cacccttttc ttggaaaaat
     2521 aatttaattc agtatgcagg tcggattcat agaaactaca aggataagtc tttggtgcgt
     2581 attttcgatt atgtggatat tcatgttcct tatttagaaa agatgtttca gaaacgacaa
     2641 gtagcttatc gaaagatgga ttatcgtgtc atcgagggtg aggagaaaca attcgtttat
     2701 gttgatagta gatatgagaa ggtgttgaga gaggacttag caggggaaag acaggaatgt
     2761 ctgcttattt taccttatgt gcaccagaca aaactgatga attttctaaa agaatttagg
     2821 attagtcaaa ttgagatatg tataccagag acggttgcaa ataaagcatg gctagaccag
     2881 ttgaagagcc agaaaattaa agtgtctttt actcaatcaa aaatagtaac gcctattctt
     2941 ttggtgaata agactattgt ttggtatggt gcaatgccat tattagggaa ggtagatgag
     3001 atgaccatat tacgtttgga atcagctagt atagtttctg aactagtggc aggtttacga
     3061 tagagaaaat ttttaaaaat ttctatgtat gattttcatt tctttagtga gactgttgcc
     3121 attatcacat tcgaatcaca caaaataaaa aaatttttat aagtacttga caaatagatt
     3181 gaaatatcat aaaataaaaa cggttacaga gttattaatt atttaagctt catgtcacca
     3241 ttaaaaattg aaataaaagg atgttatcac taatacaagt gagcaggaac ctatttaatc
     3301 acatcagaag aagtttcttg atgtttttaa gtaggttcct tttattttaa aagggaaatt
     3361 ttatgatcat aaaacgaata ctaaaccaca atgccgtaat tgcgcaaagt aaaaaagata
     3421 tcgatattct tctttttgga aggggaatag cttttggaag aaaaactgga gataaagtaa
     3481 atccaattga tattgagaaa agtttttttc tcaaaaatag agataatatg acccgtttta
     3541 cagagatgtt tattaacgtt cctttggagt tggtgtacat caccgaaaaa ataattaacc
     3601 taggtaaaat aacattgggt aataattttg atgaaattat ctatattaat ttaacggatc
     3661 atatttcttc gagcatagaa cgttataaag aagggattat tatttcgaat cccctacgct
     3721 gggaaatatc gaaatattat aaagaagaat ttgaacttgg gaaaagggct ttacaaataa
     3781 taaaaaaaga gttaggtatt gaacttccaa ttgacgaagc tgcattcata gcgctacatt
     3841 ttgttaatgc taatttagaa aataattttc aagagtcgta taaaatcact gaaataatta
     3901 tgggaattga gaaaatcatt caagatttct attgtactga gtttaaccaa gattctattg
     3961 attattatag attcataact catataaaat tatttgccca tcgcttggtt gaaaatacaa
     4021 cttattgtga cgatgatgat gaagacttgt tagcattaat gaaaaataaa tatcctagag
     4081 aatatgaatg tggtgaacag gtggcaatgt ttatacaaac ggagtacaat tatttgctga
     4141 cttctagtga attagtttat ttgatggctc atattcgtcg cttaacaaaa aatttagatt
     4201 aatagaatag gagaatacca tgagttataa agatacggta caaaaaatcc tcgatgtaat
     4261 tggaggtgaa aaaaatgtca atagagttac ccattgtgta acacgtttaa gattagaatt
     4321 aaaagatgaa aatttagtca atgatgatga tgtgaagaag ataccaggtg taataggtat
     4381 tatgaaaaag aatggacaat atcaaattat acttggtaat gatgtagcta attattataa
     4441 agaattcgtt aaacttggca attttgaatc cgattcagtt gttcaagggc acaaagggaa
     4501 tattttagaa agaatcattg agtatatcgc tggttccatg actccaatca ttccagcaat
     4561 gttaggggga ggtatgctga aagtcttggt aattatttta ccaatgcttg gtatattgca
     4621 atcagattct cagactattg cttttttgac attttttggg gatgctccat attatttctt
     4681 accgctgtta ttagcttatt ctgcatcaca aaaattaaaa gtaacatcta cattagctat
     4741 gtctgtagca ggtgtacttc tccatccaaa ttttgttcaa atggtgcaat cagggaatcc
     4801 tcttagttta tttggtgtac ctgtgacacc agctagttat ggttcgtcag tcgttccaat
     4861 tcttattatg gtttggttga tgaaatatat tgaaaaaatg attgctaaat taacaccagc
     4921 tgttactaag agttttttgc aacctacgct agtattatta gtatcaagct gtattgcctt
     4981 agttgtagtc gggcctattg gagtaattgt tggtgaagga ttatcaaatc tagttgggca
     5041 aatgtatggt gtagctggat ggcttacatt agctattctt ggtgctatta tgccatttat
     5101 tgttatgact ggaatgcatt gggcttttgc acctattttt ttggcggcat ctattgctac
     5161 tccagacgta ttaattcttc cagcaatgtt agggtcaaac ttagctcaag gggctgcttc
     5221 gatggctgtt gcattaaaga gtaaaaataa taatacaaaa caaattgctt ttgcagcagg
     5281 tttctcagcc ttacttgcag ggattaccga acctgcatta tatggtgtga ctttaaaata
     5341 taaaaaaccg ctttatgcag ctatgattgg tggtggatta gcgggattat ttgcaggtct
     5401 tactagtgtt aaagcgtatc tatttgctgt cccatctttg atagcgttgc ctcaatttat
     5461 ttattctgat gtgccatcaa atattgtaaa tgctttaatt gtggcggtta tttcggttgt
     5521 tattaccttt gtattagctt atatatttgg aatcgatgaa gaagagagtt ctagcaattt
     5581 agaagttaaa gctggagttt caaataaaaa aatgatattt tctcctatat caggagaaat
     5641 cattccgtta agcgatgtcc aggataaaac attttcagat aaactaattg gagacggagt
     5701 agcgattatt ccaagtgaag gtaaggttta tgcaccattt gatgggaaaa ttacaaatat
     5761 ttttccgact aagcacgcaa ttggattgaa gagtgatgag ggtgttgagt tactaattca
     5821 tattggatta gatactgttg agctaaaagg tcaaggtttt attagtcatg tagaagaagg
     5881 agacagagtt ttcaaaaatc agttgatttt tgaaatggac ttgaatttaa tcaagactaa
     5941 aggctacgaa acagttacac cagtaattgt aacgaatacc aatgattttc tagatgtatt
     6001 agtattacct aataatcaga caatcgagca ttctaaggaa ttactggtaa tattataatg
     6061 gaggaaagtt atgaccattt ttccagatga ttttctttgg ggtggagctg ttgcagctaa
     6121 tcaagtagag ggagcatata atgaagatgg taagggctta tcagttcagg atgtgttacc
     6181 caaaggtgga ttaggagaag caacagaaaa tcctacagaa gataacttaa aattgatagg
     6241 tattgatttt tatcataaat ataaggaaga tatatccttg ttttctgaaa tgggctttaa
     6301 tgtttttcgt acttctattg catggagtag aatttttcca aaaggagatg aggaagaacc
     6361 taacgaagct gggttgaaat attatgatga attgtttgat gaactacatg ctcacgggat
     6421 agaaccactt gtaactcttt cacactatga gactccatta tatttagcaa gaaaatatca
     6481 tggatggatt gataggagaa tgattcattt ttatgaaaaa tttgctcgaa cagttttaga
     6541 aaggtataaa gataaagtta aatattggct tacatttaac gaagtaaact ctgttttgga
     6601 attaccgttt actagtggag gaatagatat acctaaggag aatctttcga aacaagaatt
     6661 atatcaagct atacatcatg aattagtcgc ctcgagtttg gttacaaaaa ttgctcgtga
     6721 gattaattca gagtttaagg tggggtgtat ggtattagct atgccagctt atccaatgac
     6781 tccaaatcca aaagatgtat gggcgactca tgagtatgag aatctaaatt atctattttc
     6841 agatgtgcat gttagaggtt attatccgaa ttatgcaaaa agatatttta aggaaaatga
     6901 cattaacata gagtttgcag ctgaagatgc agagttatta aaaaattata ctgtagattt
     6961 tttatccttt agctattaca tgagtgtgac tcaatctgct cttccaacac agtataattc
     7021 aggagaaggg aatattattg gtggtttagt aaatccttat ttagagtctt ctgagtgggg
     7081 atggcaaatt gatccaattg gactacgtat aattttaaat agatattatg accgttatca
     7141 aattccatta tttatagtgg aaaacggatt aggtgctaaa gatcaactaa taaaagatga
     7201 atttaataac ttaacagtcc aagatgatta tagaattcag tatatgaaag aacatttatt
     7261 gcaagtagct gaagctctac aagatggtgt ggaaattatg ggctatacgt cttggggatg
     7321 tattgactgc gtttctatgt ccactgcaca gctttctaag aggtacggtc tcatttatgt
     7381 tgatcgaaat gatgatggaa atggcacatt caatcgatat aagaaaatgt cctttacttg
     7441 gtataaagga gtgattgaat caaatggaga atccttattc aaatagtttg tttattgatg
     7501 attagatatt atcacggtta aggcagcatc tcattaaata tgaaataaag aggtttggaa
     7561 gacatcaaat tctaaacctc tttattttat agggggatta attaatttaa ttttgttaca
     7621 gagagccgat tataaatagt taacttagga tagattggtt atatatagta gactgaatct
     7681 aaaatagtac gaaacaattg ctaaaacatt tatagaaatt aattttactt tcccaatcga
     7741 tttgttttca tcttatttca atccgctata aagttagata agaataagct aacaagcaga
     7801 agtaagatag gtcttgcaag aatggtaaaa atctgataaa ctaagtggta attgaataga
     7861 aatctgaaag actagtagcc caagggaagt atatcaggga ggagagccgt gactgcaagc
     7921 tctctatatg aaagctgggt gaattcactt gcgcatggat ttagaaatga aggtctgact
     7981 tgtcagatga aaacggatgg taccgcgtgt caacgctccg agtggagttt ttggcatgtg
     8041 gttttctttt tatctacgag agactgatgg aggaaatatg tcaactattg aagaacaatt
     8101 aaaagcgctt cgcgaagaaa cgctgactag cttgaagcag attactgctg gaaatgaaaa
     8161 agagatgcaa gatttgcgtg tctctgtcct tggtaaaaag ggttcgctca ctgaaatcct
     8221 caaagggatg aaagatgttt ctgctgagat gcgtccaatc atcgggaaac acgtcaatga
     8281 agctcgtgat gtcttgacag ctgcttttga agaaacagct aagctcttgg aagaaaagaa
     8341 agtcgcggct caactggcta gcgagagtat cgatgtgacg cttccaggtc gtccagttgc
     8401 gactggtcac cgtcacgttt tgacacaaac cagtgaagaa atcgaagata tcttcatcgg
     8461 tatgggttat caagtcgtgg atggttttga agtggagcaa gactactata actttgaacg
     8521 tatgaacctt ccaaaagacc acccagctcg tgatatgcag gatactttct atatcactga
     8581 agaaatcttg ctccgtaccc acacgtctcc agttcaggcg cgtgctatgg atgcccatga
     8641 tttttctaaa ggtcctttga agatgatctc gccagggcgt gtcttccgtc gcgatacgga
     8701 cgatgcgacc cacagtcacc aattccacca aatcgaaggc ttggtagttg ggaaaaatat
     8761 ctctatggct gatcttcaag gaacgcttca gttgattgtc caaaaaatgt ttggtgaaga
     8821 gcgtcaaatt cgtttgcgtc catcttactt cccattcaca gagccatctg ttgaggtgga
     8881 tgtttcttgc ttcaagtgtg gtggagaagg ctgtaacgta tgtaagaaaa caggttggat
     8941 cgaaattatg ggagccggta tggttcaccc acgtgtcctt gaaatgagtg gtatcgatgc
     9001 gactgtatac tctggttttg cctttggtct tggacaagag cgtgtagcta tgctccgtta
     9061 tggaatcaac gatatccgtg gattctacca aggagatgtc cgcttctcag aacagtttaa
     9121 ataatgatta gaaaagtaga aatggcagat gttgaggtgt tggctaaaat tgccaaacaa
     9181 gcctttcgtg aaacctttgc gtatgataat acggaagagc agttacagga atactttgaa
     9241 gaggcttata gtctgaaaac tttgtcaact gagttgggaa atcctgactc tgaaacctat
     9301 ttcattatgc atgaggagga gatagctggt tttctcaaag tcaactgggg aagtgctcaa
     9361 actgagagag aattagagga cgcttttgaa attcaacgcc tctatgtgct acaaaaattc
     9421 caaggatttg gactaggtaa gcaactgttt gaattcgcac ttgaacttgc tacaaaaaat
     9481 agtttttctt gggcttggct aggtgtttgg gagcataata caaaagctca agcgttttat
     9541 aatcgatatg gttttgaaaa atttagccaa catcatttta tggttggtca aaaagtagat
     9601 acggattggt tactgagaaa gaaattaagg taagaagagg attcttctat tgaaatgata
     9661 ggaaaggaaa agaaccagtg tggcaatggt cattctggag aactaaatta tgcttatatc
     9721 ttataaatgg ttaaaagaat tggtggacat tgatgtgcca tcacaagagt tggctgaaaa
     9781 aatgtcaact acaggaatcg aggtcgaagg tgtcgaatca ccagctgctg gtctctcaaa
     9841 aattgtcgtc ggtgaggtct tgtcttgcga agatgtgcca gagactcacc tccatgtttg
     9901 tcaggttaac gttggcgaag aagagcgtca gatcgtttgt ggtgccccaa atgtgcgtgc
     9961 tgggatcaag gtcatggtgg ctcttccaga agctcgtatc gctgataact acaaaatcaa
    10021 aaaaggaaaa atccgtggtt tggagtcact tggaatgatc tgttcacttg gtgaattggg
    10081 aatttctgac tcagttgtgc ctaaggaatt cgcagatggc atccaaatct tgcctgaaga
    10141 tgccgtgcca ggtgaggaag tcttttctta cctagacttg gatgatgaaa tcatcgaact
    10201 ttccatcaca ccaaaccgtg cagatgccct ttctatgtgt ggagtggctc acgaagtggc
    10261 agccatctat gacaaggcag tcaactttaa agaatttact ctaacagaaa ctaatgaagc
    10321 tgcggcagat gccctttctg tcagcattga gacagacaag gcgccttact atgcagctcg
    10381 tatcttggac aatgtgacca tcgcaccaag tccacaatgg ttgcaaaacc ttctcatgaa
    10441 cgaaggaatc cgtcccatca ataacgtagt ggacgtgacc aactacatcc tgctctattt
    10501 tggtcaacca atgcatgcct ttgacttgga taactttgaa gggactgaca tccgtgtgcg
    10561 tgaagcgcgt gctggtgaaa aattggtgac cttggacggt gaagaacgtg acttggacgt
    10621 gaatgaccta gtcatcactg tcgcagacaa gccagtagcc cttgcaggtg tcatgggtgg
    10681 tcaagcaaca gaaatctctg aaaaatctag tcgtgttgtc cttgaagctg ctgttttcaa
    10741 tggcaaatct atccgtaaga caagtggtcg cctgaacctt cgttctgagt catcttctcg
    10801 ctttgaaaaa ggaattaatg tggcaacagt taatgaagcc cttgatgcgg cagctagcct
    10861 gattgcggaa cttgcaggtg cgacggtgcg taagggcatc gtttcagcgg gtgagcttga
    10921 tacttcagat gtagaagttt cttcaaccct tgctgatgtt aaccgtgtcc tcggaactga
    10981 gctgtcttat gctgatgtag aagacgtctt ccgtcgtctt ggctttggtc tttctggaaa
    11041 tgcagacagc tttacagtca gagtcccacg tcgtcgttgg gatatcacaa tcgaagctga
    11101 cctctttgaa gaaattgctc gtatctatgg ttatgaccgc ttgccaacta gtctaccaaa
    11161 agacgatggt acagcaggtg aattgacagc cacacaaaaa ctccgccgtc aagttcgtac
    11221 tattgctgaa ggagcaggtt tgacagaaat catcacctat actctaacaa ctcctgaaaa
    11281 agcagttgag tttacggctc aaccaagtaa ccttacggaa ctcatgtggc caatgactgt
    11341 ggatcgttca gtcctccgtc aaaatatgat ttcaggtatc cttgataccg ttgcctacaa
    11401 cgtggctcgt aagaataaaa acttggccct ttacgagatt ggaaaagtct ttgaacaaac
    11461 aggtaatcca aaagaagaac ttccaaatga aatcaacagt tttgcctttg ccttgacagg
    11521 cttggttgct gaaaaagatt tccaaacagc agcagttcca gttgatttct tctatgctaa
    11581 gggaatcctt gaagccctat ttactcgttt gggactccaa gtaacctata cagcaacatc
    11641 tgaaatcgct agccttcatc caggtcgtac agccgtgatt tcactcggtg accaagttct
    11701 tggtttcctt ggccaagtgc atccagtcac tgccaaggct tacgatattc cagaaacgta
    11761 tgtggctgag cttaaccttt cagctatcga agctgcgctt cagccagcga ctccatttgt
    11821 agaaatcacc aaattcccgg cagtcagccg tgacgttgcc cttctcctca aggcagaagt
    11881 gactcatcaa gaagttgtag atgctatcca agctgccggc gtgaaacgtt tgacagatat
    11941 caaactcttt gacgtcttct caggtgagaa attgggactt ggtatgaagt caatggctta
    12001 tagcttgacc ttccaaaatc cagaagatag cttaacggac gaagaagtcg cacgctatat
    12061 ggaaaaaatc caagcatcgc tcgaagaaaa agtcaatgca gaagtgcgtt aaacgatagt
    12121 atgatacaaa ttccccctct agtttatgaa cactagaggg gtgattttat ataaaataat
    12181 taaaatgtga tttctgtaaa aatacatcta tgatcagagt tttgaaaact tacaatgtct
    12241 aaatctttaa catagtagtg gtttttaggc aataaaatat gatctattgt tgcattaaaa
    12301 agttttggac tttggctatt ccaagttcct ctttcaaaag gtggcagtac atttaatgcg
    12361 tccctatgag agcttatttt tgcaagtgct ccatgacgca tagttgcatt aaaatcacct
    12421 gcaataatag cctttggata ttttgaagcc aattgattat gaatgatgtt taagtcttgc
    12481 ttccagattt ccattaaacc tggcagagga ggagcagtat gcaaggcaat gatatctggt
    12541 atattttgtt ttctcgaatg taatacaatt gtcccgaacc gtgttgtatg aaaagtttta
    12601 gcttctgtat agaaaccata acttttcttg acaatcacag tcacaggagc tattccacta
    12661 ttggtaggtg gagaagtgaa aatatcatag ttggccatag aaagtccaac ttgatgaaac
    12721 aatagtttga ttctctggtt ttcttgctca cctctgatat tggtagctag ttcagggaat
    12781 atagccatat cggcgtcaaa atggctaaaa attcgctcaa tatgttgtgc ttctatttga
    12841 ttagcgacgt tccaagttac taattttaag gttttagtct tatttgatgc agaagaaagg
    12901 gttttatctg ttccgaataa tagtaatatg agcgatagta agagcaaaat gccagagtaa
    12961 aaccgtattt gtttattttt cttgtaaaaa gataggaaaa aaaataagaa acttaaaata
    13021 gcagccatag ttgcagtagc taaggggaag gcataaatct gtgccaagat aggttgggta
    13081 gatagccaaa tgttaagatt agaagatggg aaaaaagaat aagttatgat agggataaca
    13141 aagagaatga ccgaaagggc agagagaaat gttttaatca ttgtgtgagt acctcaattt
    13201 gcaatggttg ctcggagcta atgtgacttt gtgactttac ttgattgata ttctttttct
    13261 tgtcttcctc atagattaag tacaacccaa gtaaaaaaag tccaatttaa gattgaaatg
    13321 attaaaaggc acaga
//
LOCUS       DAVBDG010000050        13150 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBDG010000050 DAVBDG010000000
VERSION     DAVBDG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13150
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.051"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(278..1501)
                     /locus_tag="VQ526_001550"
     CDS             complement(278..1501)
                     /locus_tag="VQ526_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2555254.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            1669..2991
                     /locus_tag="VQ526_001551"
     CDS             1669..2991
                     /locus_tag="VQ526_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2555255.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYNKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGGIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTIENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            2993..4879
                     /locus_tag="VQ526_001552"
     CDS             2993..4879
                     /locus_tag="VQ526_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2555256.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            5047..5391
                     /locus_tag="VQ526_001553"
     CDS             5047..5391
                     /locus_tag="VQ526_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2555257.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            5401..6813
                     /gene="lacG"
                     /locus_tag="VQ526_001554"
     CDS             5401..6813
                     /gene="lacG"
                     /locus_tag="VQ526_001554"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2555258.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVREFHQLGIEGKIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            6883..8562
                     /locus_tag="VQ526_001555"
     CDS             6883..8562
                     /locus_tag="VQ526_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2555259.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPCEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(8688..10127)
                     /locus_tag="VQ526_001556"
     CDS             complement(8688..10127)
                     /locus_tag="VQ526_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2555260.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(10131..11480)
                     /gene="trkA"
                     /locus_tag="VQ526_001557"
     CDS             complement(10131..11480)
                     /gene="trkA"
                     /locus_tag="VQ526_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2555261.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVNRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11668..12516
                     /locus_tag="VQ526_001558"
     CDS             11668..12516
                     /locus_tag="VQ526_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2555262.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            12531..13079
                     /locus_tag="VQ526_001559"
     CDS             12531..13079
                     /locus_tag="VQ526_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2555263.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
BASE COUNT         4028 a         2106 c         2806 g         4210 t
ORIGIN      
        1 gagaattgta aaaacaaaaa tggggatttc atataaagtt tattctagga gtatgtatct
       61 tgaataaaaa ttgttattta agttctgggg gttatgatga atgtttttat aataataaaa
      121 tgcagctaga agaaaagtta ttttaatata aatcactcaa catagagaga aggcggtttc
      181 ctatatggaa gtcgtcttgt tttcatattg tctaaatacg agatatttac tacaggttat
      241 agttgattta aacaggtcta tagatttcta atatatatta aggtgaaata atatcttgta
      301 atacattgct atgctctatg aaaaaagcaa cgatagctaa tgcacaagct cctatacttc
      361 cacgatgttt gttgtaaggt aaaatttcaa tttctatatt acgagtaaag ggaacgaagt
      421 ggagctggtc ttggatttta tccctgactt catgagtgaa aggttgataa ttaagcaatt
      481 gactgttgat atagatttta tcagcaggat tgatgaggag aagatttgca atagaagtgg
      541 ctaataaatt gactccttta tcaatttgtt gacgtaaagc ggagtcgcct aaattataag
      601 ccgttaaaag ggtgtctaaa tgaatgtctt tttcagtctt tacaaggctt tttagtacag
      661 ttagttggga atttttaaat aataattggg cgtgtttgat taaccaagca tccgaaatat
      721 atgtttgtaa acaacctttt tttccgcatt cacaatattg cccattcaat tcgacaatgg
      781 tgtgtccaat ttctccgata taaggatttt tagaggctcc tattttttct tttgtgaaaa
      841 aggaagtgta aatacctaat ccagcgtgta ggaaaataaa gttatcgggg gtattgtgtg
      901 gattaaaaag gtattgtcct atagccatac aatcgatatt attttttaca ataaaaggaa
      961 aattgaatct tttaattaca tttaataaat taaaagattc ccatatgggg ttgtttgtaa
     1021 tgatacttcc ggagtctttg ttataatgtc caggtattga taagccaaat ccaagtaatt
     1081 ttgtttccaa agggacgtac tctatagctg tttttatcat attggtaatg gtggaattga
     1141 tttctgaaat aggataatct tcattcaaaa tttctgtctc gaaaagagtt ttcccttgta
     1201 agtcacatag actgagaaag atacctagga cgtttatttc aatacctcca acaacatacg
     1261 gaatattatc tgtgatagaa atagtttttc tacgtcttcc agatccggaa aactcgctaa
     1321 attcatcacc agtttcgtat atgagggact gttcgcttag ctcttttatg atgttagtcg
     1381 ttgttgctgg agtaagtcct gtttcattaa caatatctgt acgggatgtt gctggatttc
     1441 gatatagaaa ctccagtata gtggctctat tttgtaaaaa ggctgattgt tttgtaatca
     1501 tttgtttcct cctttggttt attttatatt aaacataaga aaaaatcaat taaaattatt
     1561 attctataca aagaaaaaaa tattgtaaaa acaacaaaaa atatattgac aacgctttct
     1621 gttagtggta tatttaattt ataaaaaata aaaaaaggag ttccggtgat gaacaatatt
     1681 ttagcgtttt tagaaacaaa agtcgctccg tttggtgaaa aagttggcaa ccaacggcat
     1741 ttgaaagcta ttcgtgaagg atttatgatg gcaatgcctt tgattttagt cggatcttta
     1801 tttcttattc taatcagttg gcctcaagag gattttacaa attggctgaa tagtgttgga
     1861 ttgctaagta tcttgacaac tatgaatcag tcaacagtag cgattatctc cttggtcgct
     1921 tgtttcggta ttgcctacag gttgtcggaa ggatatggta cagatggtcc gtcggcaggg
     1981 atcatagcct tatccagttt tgtattgatg gcacctcgtt tttcgagtat ggtttataat
     2041 aaaaatgggg agcaggtcaa gcagttattt ggcggcgcaa taccattttc tagcctgaat
     2101 gcatcttctt tgtttatggc gattactatt ggattggtta cagcagagat ttatcgtatg
     2161 tttatccagc gcggaattac gataaaaatg ccaagtggtg tcccagatgt ggtaagtaaa
     2221 tcattttcag ctcttttacc tggctttact acttttgttt tgtgggctct ggtcttaaaa
     2281 ggtcttgaag cggcaggagt tgcaggaggt ctcaacggac tcctaggtgg aattgttgga
     2341 acaccgctta agttaattgc aggaacgctt ccaggtatga ttctatgtgt tattgtaaac
     2401 tcattctttt ggttctgtgg agttaatggg ggacaagttt taaatgcttt tgtagatcca
     2461 gtttggttac aatttactat agaaaaccaa gaagctgtgg ctgcaggaca aacactccaa
     2521 cacattatta cattaccgtt taaagattta tttgtattta ttggtggcgg tggagcgact
     2581 attggtcttg cgatttgtct cttcctattt agtaagagtc gtgcgaataa aacattaggt
     2641 aagctagcta ttataccgtc tatttttaat atcaatacag ctattctatt tacgtttcca
     2701 acagttttaa atccgattat gctgattccg tttattgcta ctcctacaat caatgccttg
     2761 attacctatg tatcaatggc tgtaggatta gtaccctata caacaggtgt aatccttccg
     2821 tggacaatgc caccgattat aggaggcttc cttgcaacag gggctagttg gcgaggagct
     2881 ctattacaag ttgttttgat tttggtttct gtagcaattt attatccatt cttcaaaatt
     2941 gcagataaac gcaatcttga aaaagaaaaa gctactgttg gagggaaata agatggttat
     3001 cagagtattt gatcaacaga aaaatactta ttctagcttt gccttagagg aattaagtta
     3061 ctatatgaat cgggtcttta agactaacat agagcttgtc gaggagaagg aagcggatat
     3121 ttttgtagga ttagtcaata aagaggacag aaaagaccat gttcttatct cattagacaa
     3181 gggtaaggga agaattgagt ctaatacaat tgtaggttta cttattggaa tttaccgaat
     3241 gtttcatgaa tttggggttg tgtatactag accagggcgc agacatgact ttgttccaga
     3301 gttacgattt gaagattttt tagataaaca gctatctata gatgaaacag ccagttacta
     3361 tcatagggga gtatgtatag agggagcgga ttcatttgaa aatatactag atttcattga
     3421 ttggctacct aagattggga tgaacagttt tttcatccag tttgaaaatc cttactcttt
     3481 tttgaaacgt tggtatgaac atgaatttaa tccatatcta aataaagaac aattttcaaa
     3541 tgaattagta caagaattga gtgataggtt ggataaagaa ttgcaaaaaa gaggtcttat
     3601 tcatcatcgt gttggtcatg gatggacagg tgaagtttta ggttactctt caaaatttgg
     3661 ctgggaatca ggtcttagta tttcagagga gaagaaaccc tatgtcgctg aaataaacgg
     3721 gaaacgagaa ttgtttaata cggctccgat tttaaccagc ctggattttt caaatccaga
     3781 tgtagctgat aagatggtag aaattatcaa ggattatgcc aagaaaagac ctgatgttaa
     3841 ctacttacat gtatggttgt cggatgctcg taataatatt tgtgaatgcg aaaactgtag
     3901 acaagaattg gtttcggatc agtatattcg tattctcaat caattggata gggctttaac
     3961 gagtgaggga ttagatacaa agatttgttt tctgctttac catgagttgc tatgggcgcc
     4021 tcagaaagaa aaattagata atcctgaacg ctttaccatg atgtttgcac cgattacaag
     4081 aacatttgaa atgagttatg cagatgtaga ttttgacaat tccataccta cgcctaaacc
     4141 ttatctgcgt aataaaatta tacttccgaa ttctcttgag gaaaatttat cttatctttt
     4201 tgagtggcaa aaaacattta aaggagatag tttcgtatat gactatcctt tagggcgtgc
     4261 tcattatggc gatttaggct atatgaaaat tagtcaaacc atttacagag atgtatctta
     4321 tctttctaac ctacatttga acggttacat ttcgtgtcaa gaattacgtg ccggattccc
     4381 tcataatttt cctaattatg ttatggggga aatgctctgg aagaagacaa gaagttatga
     4441 agaattgatt gaagaatact tttctgcttt gtatgggtca aattggcagt ctgttgttga
     4501 atatttagaa aaattatcca gttattcctc atgtgattat tttaatgcaa ttggcagtcg
     4561 tcaaaatgat gttttagcga atcattatta tatagcttac aatctagctg ataatttttt
     4621 gccaattatt gaggaaaata tttctaagtt attaaatagt caaaaggatg aatggaaaca
     4681 gctcagttat catcgtgaat atgttgttaa gatggcgaag gctttatatc ttcaagcaac
     4741 cggaaaaaca aggcaagctc aagatgaatg gagaaatgtg ttgaattata tccgtgggca
     4801 cgaactgcta ttccaatcta atttggatgt ttatcgtgta attgaagtag caaaaaatta
     4861 cgctggtttc cacttataaa tcataagtat agaaaatgaa ccaaggtatt cagagaagat
     4921 tgatcctaaa tattatgaaa tttaaggatt tttaagatat ttagggtcga ctttctattt
     4981 atatcgtagc gaagtcattt taataatgat gtgtaaaaga tggatcaaga ttgaggagga
     5041 agaaagatga aatcaaaaga agaaataaat atgcttggtt ttacaattgt cgcttacgca
     5101 ggagatgcaa ggtcagattt gatggatgct ttggcgtttg cgagagatgg atattttgaa
     5161 caggcaagag aattggttga gtctgcaaac gactcaatag tgtctgccca tcgagaacag
     5221 actaatttat tagcggagga ggcatctgga gataattttg aagtgagttt tatcatgatt
     5281 catggtcaag ataccttgat gacaacgatg ctattgtatg atcaggtcaa gttttttatt
     5341 gatgaatatg aacgaattcg aaagattgaa gaacatattg gtttgcaatg aggattagtc
     5401 atggaaaatt tacaggttaa agccttaccg aaggagtttt tattaggagc tgctaccgct
     5461 gcttatcaag tagagggtgc aactagggta gatggcaaag gaacaaatat gtgggatgtt
     5521 tatttgcaag aaaacagtcc gtttttacca gatccggcta gtgattttta ttatcgttac
     5581 gaagaggata tagctttggc ggcagaacat ggtttgcagg ctttgcgttt atctatttct
     5641 tgggttcgta tatttcctga tatagatggg gagcctaata tattagctgt tcgttattac
     5701 catagagtct ttcagtcttg cttaaaacat aatgtgattc cgtttgtttc tttacatcat
     5761 tttgattcgc ctcagaaaat gttagaaaca ggggattggt tgaatagaga gaatattgat
     5821 cgtttcatac gatatgctcg cttttgtttc caagaattta cagaagtcaa gcattggttt
     5881 acaatcaatg aactgatgtc tcttgctgca ggtcaatata taggaggtca gtttcctcca
     5941 aatcatcatt ttcaattatc tgaagcaatt caagcgaatc ataatatgtt gttggcgcat
     6001 gctcttgcag tccgcgaatt tcatcaatta gggattgagg gaaagatagg ttgtattcat
     6061 gctttaaagc caggctatcc tattgatggg caaaaagaaa atattttggc agctaaacgg
     6121 tatgatgttt ataataataa atttctatta gatggaactt ttttgggcta ctacagtgag
     6181 gacacgcttt ttcacttgaa tcaaatattg gaagctaata attctagctt tattattgaa
     6241 gatggtgatt tagaaattat gaagagagct gcagctctta atacgatgtt tgggatgaat
     6301 tattatcgtt cagaatttat tcgtgaatac aaaggtgaaa atagacaaga atttaattca
     6361 acaggaataa aaggacagtc ttcttttaaa ttaaatgctc taggtgaatt tgtaaaaaaa
     6421 cctggtattc cgacaacaga ttgggattgg aatatttatc ctcaagggtt atttgatatg
     6481 ttgcttcgta tcaaagaaga atatcctcaa catccggtca tttatttaac tgaaaatggt
     6541 acagccctta aagaagttaa gccagagggc gagaatgata ttattgatga cagtaagaga
     6601 atccgttata ttgagcaaca tttacacaaa gttttagagg ctcgagatag aggagtcaat
     6661 attcaaggct attttatatg gtctttgcaa gatcaatttt cttgggcgaa tggctacaat
     6721 aagcgatatg gtcttttctt tgttgattat gaaacacaga agagatatat taagaaaagt
     6781 gctctttggg taaaagggct aaaacggaat taaggttagc gatttgactg atgtttaata
     6841 tattttaact atgagcttaa attttttaca ggaggagttt ttatggataa gttagtcgct
     6901 gccattgaaa agcaacaagg gaaatttgaa aaaatttcta ctaataatta tatgatggct
     6961 attaaagatg gatttattgc tactatgcct ttaattatgt tttcaagctt tttgatgatt
     7021 attattatga ttcctaaaaa tttcggagta gagttaccga gtccagctat tgcctggatg
     7081 aggaaagtgt atatgctaac catgggagtt ttgggtatta ttgtttcagg gacagttgca
     7141 aagtcattgg ctggaaatgt taatagaaaa atgcctcctg gaaaggtaat aaatgatact
     7201 tctgcaatgt tggcggccat atgtggctat cttgtattaa ctgtaacgct tgtagttgat
     7261 gagaaaacgg gatctacaag tttgtcgaca aactatttag gatctcaagg attgataact
     7321 tcgtttgtca gtgcctttat tactgtaaat gtttaccgat tctgtattaa gcgagacatt
     7381 actattcatt tacctaagga agttcctggg gctatatcac aagcttttag agatattttt
     7441 cctttttctt ttgttttact tattagtggt ttgttagata ttgtatctcg ttttagttta
     7501 gatgttcctt ttgcccaagt atttcaacaa ctattgactc ctatttttaa gggggcagaa
     7561 tcatatcctg ctatgatgtt gatttggttt atgtgtgctt tgctttggtt tgttggaatt
     7621 catggaccat ctattgtctt acctgctgtt acagctttac aactgagcaa tatggaagag
     7681 aatgctcaac ttcttgcaaa tgggcagttc ccttatcatt ctttaacacc taatttcggg
     7741 aattatatcg ctgctattgg aggaacgggg gctacctttg ttgtaccatt tattttgatt
     7801 ttctttatgc ggtctaaaca attaaaatcg gtaggtaaag ctacaattac tcctgtttta
     7861 tttgcggtaa atgaacctct tctatttggt atgcctgtta ttttgaatcc ctatcttttt
     7921 gtaccttttt tgatgactcc accagtgaat gtatttctag gaaaggtatt tattgatttc
     7981 tttggaatga atggatttta tatccagtta ccttgggcct ttcctggtcc cttgggattg
     8041 ttaattggaa cgaattttca acttatctcc tttgtatttt tatctttgat gttagttgtc
     8101 gacatattga tttatttgcc attctgtaga gcgtatgata gacagttact ggtgaaagaa
     8161 gatattgcaa gctcaaatga tattatttta gaggaggata caagtgaaat aatgccttgt
     8221 gagatagatg taataaaaag taaggagttg aaagtactgg ttctttgtgc agggtctgga
     8281 acaagtgcgc aattagccaa tgcaattaac gagggcgctc aattagcaga agttagagtg
     8341 attgcgaatt caggagcgta cggagctcat tatgatatta tgggtgttta tgatttaatt
     8401 attctggccc cacaagttcg gagttattat agagagatga aggtggatgc agaaagatta
     8461 ggtattcaga tagttgctac cagaggaatg gaatatattc atttaacaaa gagtccaagt
     8521 aaagccttac aatttgtatt ggagcattac caagctgtgt agtaagtttt tccatctttt
     8581 atttgagtaa agattttgct tacagatagg cttggattta aaaacgttcc ccctttttta
     8641 atataagaat ccctctttca caattgtaaa aagagggatt ttgtatttta tctcttagac
     8701 caagttctct tcataaagag aaggaggatt gggtaaatct ccaagcgccc tgcaatcatt
     8761 gcaaaggata ggagaatttt tgagatggga ctaaagattg agaaactaga agtggttcct
     8821 agaataggcc cgatattatt gaaacagcta aagacagcgc tggtcacgac cagaaaatca
     8881 ttgctatcta ggctgacaat aaagataagc gctagcaaaa tcatagcata gatgacaaag
     8941 tacttgagaa tcttatgctg ggtatctttg tcaatcaccg ttttattaac atggagggtc
     9001 aaaacacggt ggggcgatag gattgacaaa atttggtttt tggcaatttt tgaaaggatg
     9061 aggcctcgaa taatcttgag tccacctgca gttgatccag cagagccacc gattgccatg
     9121 aggaaaagga ggataaactg ggagaagagg ggccagttgg taatatctcc atatccaaaa
     9181 ccagttgttg taatgatgtt ggaaacctgg aagaaggtca tttcaaagct ctttgaaaac
     9241 cctgggtaga ggtagagggt gttgaggcta atcaagcctg tagaaaccag tacaatgacc
     9301 aagtaagccc gaagttcttc gtcaccaaag aaggccttga tgcgacggag catgaggtag
     9361 tagtagaggt tgaaatttac cccaaaaacc agaactccga tactgaccag ataggtaatc
     9421 agtgagctgc catagtgggc aattccgtcg ttatagacgg taaagcctcc agttcccgct
     9481 gtccccatag caatgacaaa actatcgtag agaggcatac cagctagata atagatgatg
     9541 acaaagaggg agaagagagc tagataaagg agatagagaa tctgggcagt gttttttagt
     9601 ttggatacaa ccttgccaaa aacaggacct ggaacctcag ccttcatcac ctctaggtgg
     9661 ctatttttgg cattgtccat aatagcaagt gcaaaaacaa gcactcccat ccctccaatc
     9721 aagtgggtaa aacttcgcca gaagaggagg gaacggctga gaaccgaaat gtcgttcaaa
     9781 atacttgctc cagtagttgt aaacccagaa ctaatttcaa aaaaggcatc aataacgcta
     9841 gggatttgcc cagaaaagac aaaggggaga ccaccaaaga aagaccaaag gatccaacag
     9901 agggcaacga tcaagactcc ctccttggca taaatccgtt gattttttgg cttctgtaaa
     9961 ctccctgaac cgcctaacaa tacgagaatc cctatggtcg aaaagagggc tgtaaagact
    10021 tggctcgatt cacggtaata gacagcgata gccacaggaa ccaagaggag aacagcttca
    10081 atcaaaagta actttgaaag gaggtaacga atcatacttt tattcatttc ttacctcgca
    10141 atcaagtcat aaatcttggt gatgtttggc aacaaggttg ttactaggag cttgtctcca
    10201 acttccaaca tatcctcccc agttgggaaa atagtcttgc cctttcgaat aatggctgca
    10261 ataagaaccc cttttttcaa tttcagttga gaaagaggtt tggcagtcat tttattggct
    10321 tccttgatat ggaattgcag ggtttcaatt tggccattgg ctagatggtg catggcttga
    10381 aggtctgaat actgggcatt aactcgacca cgaataaagt gcataatcgt atctacagcg
    10441 atgcttttag gtgtgatgat acttgaaaaa tcaggcgcat tgataatctc gaggagactg
    10501 gtacgattga ccttagtaat atttttctgt acacctaccc tgtcaaggaa catagatgta
    10561 atcagatttt cctcatcgac acctgttaga gtcgcaacgg catcatagtg ttgagcactt
    10621 tcttccagca ggatatcttt tgcggttcca tctccttgaa cgatgtagag atttgggaat
    10681 ttctcgctaa agaagctggc gatttcagga ttgatttcaa tgacttttgt atcgatacga
    10741 ctatctttga gaataccaag tagataatag gcaattctac ctgccccaac gatgagaagg
    10801 ctcttcacgg cgcgtgattt aaaataatta tggaagagta tcatatcgac acggttacca
    10861 gtgacaaaga ttctatcttt atcctgtaca gtcatgtcac cgcttggaat gataatttga
    10921 tgatccctct ctatcgcaca gacaatgaca ttaccaaatt ttttacgaaa atcagaaatg
    10981 ggcatttggc aaagaccgct ggtggacttg acgacaaatt ccatgaggct aacgcgtcca
    11041 ccagcaaagc gttcgacaga cagggcgttg gggaagtcaa tgatattcgc gatagcgcgg
    11101 gcagccaaga gctcaggatt aacgataaga gaaaaaccga gaatattctt ttccttgaaa
    11161 taagagttag aatattcagg gttccgcacc cgaacgatag tttctttagc tcccattttc
    11221 ttggctagaa ctgctgcaat catgttgact tcatcgtgct cagtcagggc gataaagata
    11281 tcacaatctt ggacgctggc ttgctcaaga atggcaaaat cggccccgtt accaaggata
    11341 ccaatgatat caaagcgatt gacaatatga ttgagaacag cttcgtcttg ctcaatcagc
    11401 aaaacatcat gcttttctgc aaccaaggag cgacagaggg caaaaccaac ttttccccct
    11461 ccgacaagga taattttcat aataaaacct actttttcat gatgtaacta tcataccctt
    11521 tttcaagaaa aaatgcacct actagctaat aacaagagct tttagtgaaa attcgctata
    11581 aggtaaaact ataccctaac caattgaaat agctattagc gactttctct gaaatatggt
    11641 atgataaagg atatacaagg agataaaatg aataataatt tactggtatt acaatcagac
    11701 tttggtctgg ttgatggtgc ggtatcggct atgattggag tggctttaga agagtctcca
    11761 accttaaaaa tccatcactt gacgcacgat atcacgcctt ataatatttt tgaggggagc
    11821 tatcgtctct ttcagacggt ggattactgg cctgagggaa cgacgtttgt atcggttgtc
    11881 gatccaggtg tcggttcgaa acgtaagagt gtagttgcca agactgcaaa aaatcaatac
    11941 attgtcacgc cagataatgg gacgctttcc tttatcaaga aacacgttgg cattgtagcc
    12001 attcgtgaga tttctgaggt ggccaatagg cgtcaaaaca cagagcattc ttataccttc
    12061 cacggtcgtg atgtctatgc ctatactggt gctaaactgg ccagtggtca cattactttt
    12121 gaggaagtag ggccagagct cagtgtggaa cagattgtag agcttccagt cgtagcgacc
    12181 atcatagaag atcatctggt gaagggagcc attgatattc tggatgtgcg tttcggttcg
    12241 ctttggacct ctatcacacg ggaagaattt tacaagctgg aaccagaatt tggtgatcgt
    12301 tttgaagtga ccatctatca tgctgatatg ctggtctatc aaaatcaggt tgtctatggc
    12361 aaatcatttg cagatgtgag aattgggcaa cccatccttt acatcaactc tctctatcgt
    12421 ttaggtttgg ctatcaacca aggttccttt gccaaggcct ataatgtagg tgtcggttca
    12481 tcttggacca ttgaaataaa gaaaatagaa ggataaaata ggagaatagt atggaaatca
    12541 aatttacaat taaacaagtt gttgctgtcg gaattggcgc tgccctcttt gtcgtcatcg
    12601 ggatgatcaa cattccaacc cctgttccaa atacaagcat ccagcttcag tatgcggtac
    12661 aagcgctact ttctattatt tttggaccga ttatcggttt gcttgtcggg ttgattggtc
    12721 atgcaatcaa ggactctctt gctggttatg gtctatggtg gacttggatt atcgctagcg
    12781 gactttttgg tctagttgtg ggacttttta gaaagtacgt tcgagtgatc aatggtgttt
    12841 ttgactggaa agatattctt atttttaacc tcattcaact acttgcaaat gcccttgttt
    12901 ggggtgtctt ggcaccactt ggagatgttg tgatttatca agaagcggca gaaaaagtat
    12961 ttgcacaagg gattgttgcg ggaattgcca atggtgtatc tgtagctatt gcaggaactc
    13021 ttctcttact tgcctatgca ggaacccaaa ctcgtgcagg aagtttgaaa aaggactaat
    13081 actcaatgaa aatcaaagag caaactagga agctagccgc aggttgctca aaacagtgtt
    13141 ttgagattgt
//
LOCUS       DAVBDG010000051        13020 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBDG010000051 DAVBDG010000000
VERSION     DAVBDG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.052"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            120..734
                     /locus_tag="VQ526_001560"
     CDS             120..734
                     /locus_tag="VQ526_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2555264.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFALILSSVVTGKVG
                     SLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAILRLVF"
     gene            838..1668
                     /locus_tag="VQ526_001561"
     CDS             838..1668
                     /locus_tag="VQ526_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2555265.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            1822..2676
                     /locus_tag="VQ526_001562"
     CDS             1822..2676
                     /locus_tag="VQ526_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2555266.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            2776..4149
                     /locus_tag="VQ526_001563"
     CDS             2776..4149
                     /locus_tag="VQ526_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2555267.1"
                     /translation="MFFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLCGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4142..5203
                     /locus_tag="VQ526_001564"
     CDS             4142..5203
                     /locus_tag="VQ526_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2555268.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5205..5897
                     /locus_tag="VQ526_001565"
     CDS             5205..5897
                     /locus_tag="VQ526_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555269.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(5927..6496)
                     /locus_tag="VQ526_001566"
     CDS             complement(5927..6496)
                     /locus_tag="VQ526_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2555270.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            6631..7245
                     /locus_tag="VQ526_001567"
     CDS             6631..7245
                     /locus_tag="VQ526_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2555271.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7247..8893
                     /locus_tag="VQ526_001568"
     CDS             7247..8893
                     /locus_tag="VQ526_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2555272.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGNTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRNFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            8905..10191
                     /locus_tag="VQ526_001569"
     CDS             8905..10191
                     /locus_tag="VQ526_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555273.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQAWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRIPITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10243..10776
                     /locus_tag="VQ526_001570"
     CDS             10243..10776
                     /locus_tag="VQ526_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555274.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRVAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            10854..11324
                     /gene="nrdI"
                     /locus_tag="VQ526_001571"
     CDS             10854..11324
                     /gene="nrdI"
                     /locus_tag="VQ526_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2555275.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHLYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11617..12879)
                     /locus_tag="VQ526_001572"
     CDS             complement(11617..12879)
                     /locus_tag="VQ526_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2555276.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3863 a         2410 c         2985 g         3762 t
ORIGIN      
        1 gttttgagca acctgcggct agtttcctag tttgctcttt gattttcatt gagtattatt
       61 aagacaatat gaggagaaag atgaaagaaa aaggatttta ggagggggcg caggcagcca
      121 tgccaacggc tcttggctat gtcagtatcg gcctggcctg tgggactatc ggtgcgccct
      181 atgtgacacc tgttgagatg ggcttgatga gtctctttgt ttatgctggg agtgcccagt
      241 ttgccatgtt ggcactgatt gtggttcaag ctcctgtggc agctattgct atgacggttt
      301 ttctaatcaa cttgcgtctc tttttgttga gtttacacgc atcgacttat ttccgtcata
      361 ccagtctttg gtacaatatc ggtatgtcta gtatcttgac agatgagacc tatggcgttt
      421 tgatgggtga attggcccat acagacaagg taaatcctat gtggatgcac ggaaacaatc
      481 ttaacagcta tgtggcttgg tttgtgggga cagtagtcgg aacggctctg ggtggcctgc
      541 taccaaatcc agaaatcttt gccttgattc tttcaagcgt agtgacaggt aaggttggga
      601 gccttcctca aattaaatgg ctagacttct tagccgtctt tccaacagct tgggtagcct
      661 ttcgctaccg caatctagtc ggaacagttc tctttggagt ggtcttgatt gccatcttgc
      721 gtttggtctt ttaaattacc cataaaaaaa acttatcaca gagatagata tcatataatg
      781 gcgtaaatgc tctttttctg ttaagattat aaggtattct attttggagg aaatgacatg
      841 aaaaaaatcg ttaaatactc atctcttgct gccctaggac ttgttgctgc aggtgtgctt
      901 gcggcttgct cagggggtgc taagaaagaa ggagaagcag ctagcaagaa agaaatcatc
      961 gttgcaacca atggatcacc aaggccattt atctatgaag aaaatggcga attgactggt
     1021 tacgagattg aagtcgttcg cgctatcttt aaagattctg acaaatatga tgtcaagttt
     1081 gaaaagacag aatggtcagg tgtctttgct ggtcttgacg ctgaccgtta caatatggct
     1141 gtcaacaatc ttagctacac taaagaacgt gcggagaaat acctctatgc cgcaccaatt
     1201 gcccaaaatc ctaatgtcct tgtcgtgaag aaagatgact ctagtatcaa gtctctcgat
     1261 gatatcggtg gaaaatcgac ggaagtcgtt caagccacta catcagctaa gcagttagaa
     1321 gcatacaatg ctgaacacac ggacaaccca actatcctta actatactaa ggcagacttc
     1381 caacaaatca tggtacgttt gagcgatgga caatttgact ataagatttt tgataaaatc
     1441 ggtgttgaaa cagtgatcaa gaaccaaggt ttggacaact tgaaagttat cgaacttcca
     1501 agcgaccaac aaccgtacgt ttacccactt cttgctcaag gtcaagatga gttgaaatca
     1561 tttgtagaca aacgcatcaa agaactttat aaagatggaa ctcttgaaaa attgtctaaa
     1621 caattcttcg gagacactta tctaccggca gaagctgata ttaaataatt tcttgaaatc
     1681 atccattctg agtaaggtgg gtgttttctc agtttttagg gatatagttt taaactatat
     1741 atctgactat ctaataacaa tttgtgaaat caaacaaatt gtgagatact agtacggtat
     1801 tatttttaag gagaaagaat catgaaaatc aaaaaatggc ttggtctagc agcccttgct
     1861 acagtcgcag gtttggctct tgcagcttgc ggaaactcag aaaagaaagc agacaatgca
     1921 acaactatca aaatcgcaac tgttaaccgt agcggttctg aagaaaaacg ttgggacaaa
     1981 atccaagaat tggttaaaaa agacggaatt accttggaat ttacagagtt cacagactat
     2041 tcacaaccaa acaaagcaac tgctgatggc gaagtagatt tgaacgcttt ccaacactat
     2101 aacttcttga ataactggaa caaagaaaac ggaaaagacc ttgtagcgat tggagatact
     2161 tacatctctc caatccgcct ttactcaggt ttgaatggaa gtgccaacaa gtacactaaa
     2221 gtagaagaca tcccagcaaa cggagaaatc gctgtaccga atgacgctac aaacgaaagc
     2281 cgtgcgcttt acttgcttca atcagctggc ttgattaaat tggatgtttc tggaactgct
     2341 cttgcaacag ttgccaacat caaagaaaat ccaaagaact tgaaaatcac tgaattggac
     2401 gctagccaaa cagctcgttc attgtcatca gttgacgctg ccgttgtaaa caataccttc
     2461 gttacagaag caaaattgga ctacaagaaa gcacttttca aagaacaagc tgatgaaaac
     2521 tcaaaacaat ggtacaacat cattgttgcg aaaaaagatt gggaaacatc acctaaggct
     2581 gatgctatca agaaagtaat cgcagcttac cacacagatg acgtgaaaaa agttatcgaa
     2641 gaatcatcag atggtttgga tcaaccagtt tggtaataag aaacagggag gtgggagaga
     2701 aaattccacc tcttgctttt gtatagagta tagattgtaa agaagactat tcgttcatag
     2761 aaaggtagag agaatatgtt ttttcctagc gaacaagaac agattgaaaa atttgaaaag
     2821 gatcatgtag cccagcatta ttttgaggtt ttgcgtacct tgatttctaa gaaatcagtc
     2881 tttgcccagc aggttggact caaggaagtc gcaaactatc tgggtgagat tttcaagcgt
     2941 gttggagctg aagtggagat tgatgagagc tatacagcgc cctttgtcat ggcacatttc
     3001 aagagttcgc gtccagatgc caagaccttg attttctata accactatga cactgtgcca
     3061 gcggatgggg atcaggtctg gacagaggat ccttttacgc tttcggtccg caatggcttc
     3121 atgtatgggc gtggggttga tgacgacaag ggtcatatca cagctcgctt gagtgctttg
     3181 agaaaatata tgcagcacca tgatgattta cctgtcaata tcagctttat catggaggga
     3241 gcggaggaat cggcttcaac agacctagat aagtatttgg aaaagcatgc agacaaactc
     3301 tgtggggcgg atttgttggt ctgggaacaa gggaccaaaa atgccttgga acagctggaa
     3361 atttctggtg gcaataaggg aattgtgacc tttgatgcca aggtaaaaag cgctgatgtg
     3421 gatatccact cgagttatgg tggtgttgtg gaatcagctc cttggtatct cctccaagcc
     3481 ttacagtctc ttcgtgctgc ggatggccgt atcttggttg aaggcttgta cgaagaagta
     3541 caagagccca atgaacgaga aatggccttg ctagaaactt atggtcaacg aaacccagag
     3601 gaagttagtc ggatttatgg attggagttg cctctcttac aggaggagcg gatggccttt
     3661 ctaaaacgtt tctttttcga gccagcgctt aatatcgaag gaatccagtc tggttatcaa
     3721 ggtcagggtg ttaagactat tttacctgca gaagccagtg caaagctaga ggttcgtctg
     3781 gttccgggcc tagaaccgca tgatgttctg gaaaaaattc ggaaacaact agacaaaaat
     3841 ggctttgata aggtagaatt atactatacc ttgggagaga tgagctatcg aagcgatatg
     3901 agcgcaccag ccattctcaa tgtgatcgag ttggccaaga aattctatcc acagggtgtt
     3961 tcagtcttgc cgacgacagc ggggacagga cctatgcata cggtctttga tgccctagag
     4021 gtaccaatgg ttgcattcgg tctaggaaat gccaatagcc gagaccacgg tggagatgaa
     4081 aatgtgcgaa tcgctgatta ttacacccat atcgaattag tagaggagct gattagaagc
     4141 tatgagtaga gatattatca agttagatca gatcgatgtg acttttcacc aaaagaagag
     4201 aaccattaca gcggttaagg atgtgaccat tcacatccaa gaaggggata tctacggaat
     4261 cgttggatat tctggagcag gaaaatcaac ccttgtacgg gtgattaacc tcttgcaaaa
     4321 accatctgca gggaaaatta ccattgacga cgatgtgatt tttgatggta aggtcacctt
     4381 gacggcagag cagttgcgtc gtaaacgtca agatatcggg atgattttcc agcattttaa
     4441 cctgatgagc caaaagacag cagaggagaa tgtagccttt gcccttaaac actctggact
     4501 cagcaaggaa gaaaagaagg ctaaagtagc taagttgttg gacttggttg gtttggcaga
     4561 tcgtgctgaa aactaccctt cacaattatc tggagggcaa aaacagcgtg tggcaattgc
     4621 gcgtgccttg gccaatgatc caaaaatctt gatttcagac gagtcaactt ctgcccttga
     4681 ccctaagaca accaagcaga ttttggcctt gttgcaagat ttgaaccaaa aattaggctt
     4741 gactgttgtc ttgattacgc atgaaatgca gattgtcaaa gacattgcca accgtgttgc
     4801 agttatgcag gatgggcatt tgattgaaga gggtagtgtg cttgaaatct tctcaaaccc
     4861 taaacaacct ttgactcaag actttatctc aacagctaca ggtattgacg aagccatggt
     4921 caaaatcgag aagcaagaaa tcgtggaaca cttgtctgaa aacagtctct tggtgcaact
     4981 caagtacgct ggagcttcaa cagacgagcc acttttgaat gaattgtaca agcattacca
     5041 agtaatggct aatattctct atgggaatat cgaaattctc gatggtactc ctgttggaga
     5101 attggtggtg gttttgtcag gtgaaaaagc agcgttggca ggtgcccaag aagccattcg
     5161 tcaagcaggt gtacaactaa aagtattgaa gggagtacag taagatggaa tcattgattc
     5221 aaacctattt accaaatgtc tataagatgg gttgggctgg tcaggcaggc tggggaacgg
     5281 ctatctactt aactctttat atgacagttc tttccttcat tatcggaggc ttcttggggc
     5341 tagtggcagg tctctttctc gtcttgacag cgccaggtgg tgtcttggag aataaagtcg
     5401 tattctggat tttagacaaa attacctcaa tttttcgtgc ggttcccttt atcatcctct
     5461 tggcaatctt gtcaccactt tctcacttga ttgttaaaac aagtatcggg ccaaatgcag
     5521 cccttgtccc actttctttt gcagtctttg ccttctttgc ccgtcaggtg caggttgtct
     5581 tggctgaatt ggatggcggt gtcattgagg cggctcaagc gagcggagcg actttctggg
     5641 acatcgtggg tgtttaccta tcagaaggtc ttccagattt gatccgtgtg acgactgtga
     5701 ccttgatttc ccttgttggg gaaacagcta tggccggtgc ggttggagct ggtggtatcg
     5761 gtaacgtagc catcgcttat ggatttaacc gctacaatca cgatgtgacc atcttggcaa
     5821 ccattattat cattttgatt atctttgcaa tccaattctt gggagatttc ttgactaaga
     5881 aattgagcca taaataaaaa agagccgcgt ggctcttttt aactgatcag attttctgtg
     5941 caaatttttt actcaaggct tgtccaatca aagcacccac tagggctccg atgacaatac
     6001 ttgcgataaa tagaaggaca gttccagggt ttggagcgac catgatacgg tcgatatatt
     6061 cttgggattt tcctcttgcc agaagagtag ccatataggc tttgggcgca atccacataa
     6121 gcaagattgg tcctgttgta ctaaaggcga aaataatgaa agaaaggaag ttctttgttt
     6181 tgtccttgta ttttcctaaa tgagctactt catctgctag gaggccacag ataattccag
     6241 gaaggaagga accagcaccg tgtttagttc ccaagaaaaa gaaggcaatg acaaggccga
     6301 tagtggtaat ggctccaaag cgcggaactt ttgcgactag gatcatatag acgctaccgc
     6361 cgacaagggc agtaaaggca ggcgcataaa acatgtttcc agtttggtca aagagattgc
     6421 ccaaaaggac accaatcccc atgcagagga agtaaagaac tgcaaaaagc agtgtagtta
     6481 agatagattt cttcatgaat tgtctcctga taattttttc acaattctca ttttaccatg
     6541 gtttggtggg attgtaaatg gatactagaa gtttttgaaa acgcttgaaa tatgatataa
     6601 tggtttcata gttattttta ggggaatatc atgggtagat ttttagactt tgtctttaat
     6661 cgtttctttt tagggatgat tgcgacagcc ttcttttggc tattaacttt agcaggaggg
     6721 attatccttg gtctagcgcc ggctagtgcc accttgatga gcttatatgc agaacatggt
     6781 tatagctttc gggaatacag tttgaaggag gcttggtctc tttacaagca aaattttgtc
     6841 tcaagcaacc tgattttcta tagcttttta ggtgtgggtc tagttttgac ctatggtttg
     6901 tatctcttgg tgcaattgcc tcatcagacc attgttcatt tgattgcgac ccttttgaat
     6961 gtcttagtag ttgccctgat ctttttggct tatacagtat ctttaaaatt acaagtttat
     7021 tttgccttgt cctatcgaaa tagtctcaaa ttatccttga ttggcatctt tatgagtcta
     7081 gcagctgtgg ccaaggttct ccttgggact gtgctacttg tagcaattgg ttactatatg
     7141 cctgccctgc tattttttgt aggaattggg atgtggcatt tctttatcag tgatatgttg
     7201 gaacctgtct atgaaatcat ccatgaaaaa ttggcgacaa aatagaatga agcagttttg
     7261 gctacatacg cttctaagaa cctatagttc agtgatgatc attatcattg cgagttttgc
     7321 aatcttactc tcttacgctg actgggattc acgtgaaaag gaagcccaga gagtagccca
     7381 gcgtgtaact gctagaacag tgagtgaaat tgaatattac catagagagt caacccagat
     7441 agctcaggct ttagttgaaa atcaagctcg tattgaggga atctataaat actttagcct
     7501 tagcatgcca gactattttt actggcaatt agagcggaaa gcttcgcctt atatatcagt
     7561 ctctctgtat gaaaatgttg atgacctcta tgttcgaaat gattttgtaa ctggagtggc
     7621 cattgctttt caagattaca aggaagtcta tgtttctact aaagacaaac gtagtggaga
     7681 aaaaatcagg gctgaggatt tcaaaccagc aggaaatagt tttgccattc cggtgtcaga
     7741 tccagtgtca gatcaagatt taggagtgat ttacatctcc ttggatcctg ctgttttata
     7801 ccatgccatt gataatacta gaggtaatac tccgatggca gtaacagtga cctcaccttt
     7861 tgatacggag atttttcata ttggtgagac agttgataag gagagtgaaa attggctagt
     7921 tggcttaact tctcatggat atcaggttca ggtggcagtt cctaaaaact ttgttttaca
     7981 aggaacagtg actagctctg ctttgattgt gggtttgagc cttctcttta ttgtcattct
     8041 ttatctgact ttgaggcaga cttttgctaa ttaccaaaag caggtagtgg atttagtaga
     8101 atccattcaa gtcattgctc aaggcgaaga gggtcgtcgg attgacattt ccgagaaaga
     8161 tcaggaatta ctcctaatcg cggagacgac caatgatatg ttggatcgat tggaaaagaa
     8221 tatccatgat atttaccagt tagagcttag tcaaaaagat gctaatatgc gagccttgca
     8281 ggcgcaaatc aatcctcatt ttatgtataa tacgctggag ttcttgcgca tgtatgcagt
     8341 tatgcagagt caagatgagt tggcagatat catttatgaa ttcagtagtc tcttgcgtaa
     8401 caatatttcc gacgaaagag agaccctcct caaacaggaa ttagaatttt gccgtaaata
     8461 cagctatctc tgcatggttc gctatcccaa gtccattgcc tatggtttca agatagatcc
     8521 agagttagag aatatgaaga ttcctaagtt caccttgcaa ccgctggtag aaaattattt
     8581 cgcgcatggt gttgaccaca ggcggacaga taatgtgatt agcatcaagg ctcttaaaca
     8641 ggatggtttt gtggaaattt tggtggtcga taatggtaga ggaatgtcgg ctgaaaagtt
     8701 ggcaaatatc cgagaaaaat taagtcagag aaattttgaa caccaagcca gctacagtga
     8761 tcaaaggcag tctatcggga ttgtcaatgt acacgagcgt tttgtgctct attttggaga
     8821 ccgctatgcc attactatag agtctgcaga gcaagccggt gttcagtatc gtattacaat
     8881 tcaagatgag tagaaaggga gaaaatgtat aaagtattat tagtagatga tgagtacatg
     8941 gtgacagaag gtctgaagcg tttgattccc tttgataagt gggatatgga ggtcgtcgca
     9001 acagccagtc atgccgatga agctctagaa tatgttcagg aaaatcctgt cgatgtcatc
     9061 atttccgatg tcaatatgcc agacaaaaca gggcttgata tgattcggga gatgaaagag
     9121 atcttaccag atgctgccta tatcctgctc tcaggttatc aggagtttga ttatgtaaaa
     9181 agagcaatga accttagtgt ggtggactat ttggtcaaac ctgttgataa ggtagagctg
     9241 ggaaatctgc tggagaagat tgcaggtcag ctcggcgaga gagggaagaa aagtcagact
     9301 cttagtcaag aattagacga ggctggattt gttagttatt taggggataa ggagaattgg
     9361 tggataggtc tatccaagga aaaacaaggt tccttcacca ttccctacta tgtcttgggt
     9421 caagcctggc agattttcat ttctgaccaa cccctagatg gtttagtcgt tacacctttt
     9481 gaagctcctt atcaagaaca ttttgaacgc tggaagctga atgctgagaa aaccctcttt
     9541 tacggttctg taaatctgca gcagtctgag agtctctttg cctattacga accgatttat
     9601 agggttatca ttcagggaaa tctcaatcaa atcgtagaag agttaaatct cttggagaag
     9661 gtagttcttg aaaatacgcc gcgaattccg attactaaac agctttttat ccagtttgtc
     9721 atggatgtct tccatttatt tgaacatctc aaagctgatg atatgacgga cattgtcaaa
     9781 accattcatg ctattcaatc cttcgatgaa ttggtttctt atatcaagga aactctgatc
     9841 agctttttcg gtcaataccg tatgaatgaa aatgtggtca gtgtgctgga agtcattggt
     9901 cgtgattacc aaaaagagct ttccctcaag gatatcagta aggccctctt tatcaatcct
     9961 gtctatctag ggcagttgat taagcgtgaa accgattcga cctttgcaga gttactaaac
    10021 aaacaacgta ttaaggctgc ccagcagctc ttgctttcaa ccagtgacag tatcgaagat
    10081 atttgttatg ctgttggtta cagtaacctt ggatatttct ataaagtttt ccgaaaattg
    10141 tgcggaaaat cgccaaaagc ctaccgaaaa caggtagaaa ctatactata agatttgtat
    10201 tcctttacaa aatgtgctat aatattatca ggaggttcta tcatgaaaaa gaaaccaatt
    10261 tatctatggg tcttgctaat cttgtctgcc cttatttcag ttccgtctct gtttggaatt
    10321 gtgagtccct tgcctagtaa agaagccctt cgtgttgctc agaaacaagt tgcaggggtc
    10381 aatgctcagc aattagaaga ccagcttaat tatacctata gagtagcaga agcatctcat
    10441 tctattttta atgttgcttt gattgtgcta tctactattt tagtggtcgt agcgattgtc
    10501 ttccttgttc gtaaaaattt gcaatatgca aactatactt acgttggcta tgttttgtta
    10561 gctattattg gttcgattta tggctatgtt ggtttacaag acgctgtgca gttggttcaa
    10621 gatgagagca tgcgtttgac agtgagtatt ggttcaaaag ctgttagcat tttctatatc
    10681 gtcatcaatg ttctcttcct agcccttgtc ttctataaga tgtggcgaca acagaaagcc
    10741 ttggcagaag aagaggaaac agaagagctt gcctaagtat tgacaaaaaa gaactatcaa
    10801 gatacaatct tggtagttat tttttgatta tagataaaat catggaggta catatgaaga
    10861 caatttcttt agtttatatt agtttgagtg gcaacactga aagttttgtg acgcgcttga
    10921 aagactatct cttgtcccag tacaaaggga ttgaggttca aaagattcat atcaaggatt
    10981 tggtcaagga aggcaaaaat ttctatgaaa tggaccatct ctatgtcgct tttttgccga
    11041 cctatctcga aggtgggaat ggcgtggata acggagatgt tgagattttg acaacaccgg
    11101 tgggagattt tattgcctat ggtaacaatg ctagtaagtg ttttggtgtg gttgggtcag
    11161 gaaatcgtaa ctttaataac caatactgcc taacagccaa gcaatacagt caacgctttg
    11221 gcttccctgt attggctgac tttgaaatgc gaggcatgct ggaagatatc aaacatgtcg
    11281 cagctattat cgcagatttg tatgagttgg aaaaggagaa ttaatactca atgaaaatca
    11341 aagagcaaac taggaagcta gtcgcaggtt gctcaaagca cagctttgag gttgcagata
    11401 gagctgacgt ggtttgaaga gattctcgaa tagtataaag ttcttataag aggtggttga
    11461 agttgttcaa ctgttttttg agtataaaca gtctttaaga acgtaaaaaa gcataagttc
    11521 aggtcttcaa aaccttgttc ttatgctttt tatcatagaa atatttgctg aattttctct
    11581 tgaaatgaaa ccttatcttc atctcgcgct tgtcccttac cctgcttttc cgattgcgag
    11641 tgcgtagata aagaagatgg cgaagccaaa gaggaaaatc aatcccgcaa tggcaaggtg
    11701 tttgagccaa gaaggaagat ttttgttttc acgcgttacc aaggcgtaat tcaaaagagc
    11761 aaagaaaggt gttgtcagga aagatccaat catggcaaag cggagcatgg ttgaaacctg
    11821 accagcgaag aacttgataa tgacgatacc gatgatagca gtgatggtca tccagatgtt
    11881 caaagattta cgattgtcct ctttttgact gattagcagt cggagagatt cctgattaac
    11941 gcgagaatag ccatcgataa cagttataac tgttccaaag atacagagga aggcaataaa
    12001 ggtaatcaag taacgggacc attcgccaag aacagaggca tacatgccca cgaattgaga
    12061 gatgtatttg gctgaagcag cttcaaccgc ctgccctgta ggatactgaa tcagtgctcc
    12121 cagtgccaca aagaagacgg ctaggatagc tgttccaata taaccagtgt taaagtcaaa
    12181 cagagcgtct tctgtgttaa agttgacggt ctttctcttt tcagctgacc aaagtgaatt
    12241 gatagctgaa atttcaatag gagccggcat ccatcctagg agggagacga tgaagggcag
    12301 agctgccatt tgccaaggtg tcttctcgac aaaatcagaa ctgtattctg gatgcttgac
    12361 cgctgctatg ataactgcca gaacagtcgc aatggttaag gcagacataa tccatttgac
    12421 catgccgtct aaaagtttgt agcctccaaa gagtagcata gcccaaatga ttgcaacgag
    12481 aatgagggac cactgagtaa tgctaagtcc aatcattggg aaggcactgg cgatgatagc
    12541 tgagcacaga atggcaacac cagccgtgtt gaccatagcc gaaaagacat tgaggataaa
    12601 gaaaatccag agatagagtt ttcctttttc ggcataacct tcaaccaaag tctttccagt
    12661 atcagctgtg tattcagcac caaaacggaa aaatggatat ttaaagacat tggctaagat
    12721 gactaagaga agtagagacc aaccgtaaga accgccagct tgagttgagg atacaatgtg
    12781 ggaacctcca acagcggcag ttgccattag gattccaggt cccattgctt ttagcttgct
    12841 tgcccaggtt gattgattta aggaaatagc ttgcgacatg ataaatactc ctttttgaat
    12901 tttcagaata atctgtatat gtaatctata ttctacaaaa aattttggaa aaatgcaaga
    12961 atattttgaa aaaagataga aaatttacaa agaaaatctg aaataactgt tatgatatag
//
LOCUS       DAVBDG010000052        12573 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBDG010000052 DAVBDG010000000
VERSION     DAVBDG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.053"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQ526_001573"
     CDS             complement(251..433)
                     /locus_tag="VQ526_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555277.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQ526_001574"
     CDS             complement(649..1881)
                     /locus_tag="VQ526_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2555278.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ526_001575"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ526_001575"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2555279.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQ526_001576"
     CDS             complement(3048..4217)
                     /locus_tag="VQ526_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2555280.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQ526_001577"
     CDS             complement(4403..5707)
                     /locus_tag="VQ526_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2555281.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQ526_001578"
     CDS             complement(5824..6978)
                     /locus_tag="VQ526_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2555282.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQ526_001579"
     CDS             complement(6975..7526)
                     /locus_tag="VQ526_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2555283.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQ526_001580"
     CDS             complement(7519..8655)
                     /locus_tag="VQ526_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2555284.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ526_001581"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ526_001581"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2555285.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ526_001582"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ526_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2555286.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQ526_001583"
     CDS             complement(10172..10903)
                     /locus_tag="VQ526_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2555287.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQ526_001584"
     CDS             complement(10905..12350)
                     /locus_tag="VQ526_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2555288.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4237 a         2434 c         1654 g         4248 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacca tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcatccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttt ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactacat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaaa tatagtaaaa
    12481 tgaaataaga ataggacaaa tcaatcagga cagtcaaatc gatttctaac aatgttttag
    12541 aatcagatgt gtactattct agtttcaata taa
//
LOCUS       DAVBDG010000053        12163 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBDG010000053 DAVBDG010000000
VERSION     DAVBDG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.054"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            172..2463
                     /gene="pcrA"
                     /locus_tag="VQ526_001585"
     CDS             172..2463
                     /gene="pcrA"
                     /locus_tag="VQ526_001585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2555289.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDTDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2506..3186
                     /gene="radC"
                     /locus_tag="VQ526_001586"
     CDS             2506..3186
                     /gene="radC"
                     /locus_tag="VQ526_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2555290.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3183..3872)
                     /locus_tag="VQ526_001587"
     CDS             complement(3183..3872)
                     /locus_tag="VQ526_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2555291.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3890..4531)
                     /locus_tag="VQ526_001588"
     CDS             complement(3890..4531)
                     /locus_tag="VQ526_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2555292.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4614..4829)
                     /locus_tag="VQ526_001589"
     CDS             complement(4614..4829)
                     /locus_tag="VQ526_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2555293.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4837..5184)
                     /locus_tag="VQ526_001590"
     CDS             complement(4837..5184)
                     /locus_tag="VQ526_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2555294.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKEVTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5189..6304)
                     /locus_tag="VQ526_001591"
     CDS             complement(5189..6304)
                     /locus_tag="VQ526_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2555295.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFTNNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6314..7273)
                     /locus_tag="VQ526_001592"
     CDS             complement(6314..7273)
                     /locus_tag="VQ526_001592"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2555296.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7386..7955)
                     /locus_tag="VQ526_001593"
     CDS             complement(7386..7955)
                     /locus_tag="VQ526_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2555297.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8083..8754
                     /locus_tag="VQ526_001594"
     CDS             8083..8754
                     /locus_tag="VQ526_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2555298.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8738..9556
                     /locus_tag="VQ526_001595"
     CDS             8738..9556
                     /locus_tag="VQ526_001595"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2555299.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9553..10449
                     /locus_tag="VQ526_001596"
     CDS             9553..10449
                     /locus_tag="VQ526_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2555300.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10493..11467
                     /gene="pta"
                     /locus_tag="VQ526_001597"
     CDS             10493..11467
                     /gene="pta"
                     /locus_tag="VQ526_001597"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2555301.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFKEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(11783..>12163)
                     /locus_tag="VQ526_001598"
     CDS             complement(11783..>12163)
                     /locus_tag="VQ526_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2555302.1"
                     /translation="LTNKEILDKLLSYSQDLKHHYQLYQLLLFHFQNKEPEKFFGLIE
                     DNLKQVHPLFQTVFKTFLKDKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFE
                     NFKKRIFTALNIKKERTKFVLSRA"
BASE COUNT         3539 a         2319 c         2617 g         3688 t
ORIGIN      
        1 aggtattgag ctagtttatg aaaaaattgg gtgaaaagtc gagtgtttta gaaacccaca
       61 gtgtagtatt ctagtttcaa tccactatat tttgctactc cccgtaaagt ttctattttc
      121 cctgatttct gatataatag aaatattgac ttcaagagta aggaagagaa gatgaacgca
      181 ttattaaatg gaatgaatga ccgtcaggct gaggcggtgc aaacgacaga aggtcccttg
      241 ctaatcatgg caggggctgg ttctggaaag actcgtgttt taacccaccg tatcgcttat
      301 ttgattgatg aaaagctggt caatccttgg aatatcttgg ccattacctt taccaacaag
      361 gctgcgcgtg agatgaaaga gcgtgcttat agcctcaatc cagctactca ggactgtctg
      421 attgcgacct tccactccat gtgtgtgcgt attttgcgtc gcgatgcgga ccatattggc
      481 tacaatcgta attttacaat tgtggatcct ggtgaacagc gaacgctcat gaaacgtatt
      541 ctcaaacagt tgaacttgga tcctaaaaaa tggaatgaac gaactatttt ggggaccatt
      601 tccaatgcta agaatgattt gattgatgat gttgcttatg ctgcccaagc tggcgatatg
      661 tatacgcaaa ttgtggccca gtgttataca gcctatcaaa aagaacttcg tcagtctgaa
      721 tccgttgact ttgatgattt gattatgctg accttgcgtc tctttgatca aaatcctgat
      781 gttttaacct actaccagca aaaattccaa tacatccacg ttgatgagta ccaagatacc
      841 aaccacgctc agtaccaatt ggtcaaactc ttggcttccc gttttaaaaa tatctgtgtg
      901 gttggggatg cggaccagtc tatctacggt tggcgtggtg ctgatatgca gaatatcttg
      961 gactttgaaa aggattaccc caaagccaag gttgttttgt tggaggaaaa ttaccgctca
     1021 accaaaacca ttctccaagc ggccaacgag gttattaaaa acaataaaaa tcgccgtcct
     1081 aaaaatctct ggactcaaaa cgctgatggg gagcaaatcg tttactatcg tgccgatgat
     1141 gagctggatg aggctgtatt tgtagccaga accatcgatg aacttagtcg cagtcaaaac
     1201 ttccttcata aggattttgc agttctctat cggactaatg cccagtcccg tacaattgag
     1261 gaagccctgc tcaagtctaa cattccttat accatggttg gcggaaccaa attctacagc
     1321 cgtaaggaaa ttcgcgatat tattgcttat ctcaacctta ttgctaattt gagtgacaat
     1381 attagttttg agcgtattat caacgagcct aaacgtggaa ttggtccagg tacagttgag
     1441 aaaatccgtg attttgcaaa tttgcaaaat atgtctatgc tggatgcttc tgctaatatt
     1501 atgttgtctg gtatcaaggg taaggcagcc caatctatct gggattttgc caatatgatg
     1561 cttgatttgc gggagcagct agaccactta agcattacag agttggttga gtccgtccta
     1621 gaaaaaacag gttatgtcga tattcttaac gcccaagcga ctctagaaag caaggcacgg
     1681 gttgaaaata tcgaagagtt tctttctgtt acgaagaact ttgatgacac cactgatgtg
     1741 acagaagagg aaactggtct ggacaaactg agtcgtttct taaatgactt ggctttgatt
     1801 gccgacacag attcaggtag tcaggagaca tcagaagtga ccttgatgac cctgcatgct
     1861 gccaaaggtc tcgaatttcc agttgtcttt ttgattggga tggaagaaaa tgtctttcca
     1921 cttagtcgtg cgactgaaga tacagatgaa ttagaagaag agcgccgtct agcctatgta
     1981 ggtatcacgc gtgcagagaa aattctctat ctgaccaatg ccaactcacg cttgcttttt
     2041 ggtcgtacca attataaccg tccgactcgt tttattaacg aaatcagttc agacttgctt
     2101 gagtatcaag gtctggctcg tcctgcaaat acaagcttta aggcatcata tagcagtggt
     2161 agtatttcct ttggtcaagg tatgagtttg gctcaggctc ttcaagaccg taaacgcggt
     2221 gctgccccaa aatcaatcca gtcaagcggt cttccatttg gtcaatttac agctggcgca
     2281 aaatcagcat ctagcgaggc aaattggtcc attggtgaca ttgctctcca caagaaatgg
     2341 ggagagggaa ctgttctgga agtttcaggt agcggtgcta ggcaggaatt gaaaatcaat
     2401 ttcccagaag taggtttgaa aaaactttta gccagtgtgg ctccaattga gaaaaaaatc
     2461 taattttcca tccttctcac gaataataaa gtgaggagga tttttatgta cagtatttca
     2521 ttccaagaag attcactatt accaagagaa aggctggcca aggaaggagt tgaagcgctt
     2581 agtaaccaag agttgctagc tattttactt aggacaggaa cacgtcaagc tagcgttttt
     2641 gaaattgccc aaaaagtctt gaacaatctt tcaagcctaa cggatttgaa aaaaatgacc
     2701 ctgcaggaat tgcagagttt gtctggtatt gggcgtgtta aggccataga attacaagct
     2761 atgattgaac tggggcatcg tattcacaaa cacgagactc ttgaaatgga aagtattctc
     2821 agcagtcaaa agttggccaa gaagatgcag caggaattag gggataaaaa acaagagcac
     2881 ctggtggcac tctatctcaa tactcaaaat caaatcatcc atcagcagac catttttatc
     2941 ggttctgtaa ctcgtagtat cgctgaaccg cgagagattc tgcactatgc aatcaagcat
     3001 atggcgactt ctcttatctt ggtccacaat catccttcag gagcggtagc gcctagccaa
     3061 aatgatgatc atgtcactaa acttgttaaa gaagcctgcg aactgatggg gattgttctc
     3121 ttggaccatt tgattgtctc tcattctaat tactttagtt atcgtgaaaa gacagattta
     3181 atctaaagtt cattaacgac atagtcaaag agttttttat ctttgggacg attttcaaaa
     3241 agaagttctg gatgccattg gacaccgaga aaggcgacat catccgtact catgacagcc
     3301 tcaatgatac catctttagg atcatgagcc acaactttta aatttggtgc taagtccttg
     3361 atgctctggt ggtggaagga gttgatatga gagatttctc catagatttc ttggagaacg
     3421 gtatctggtt ctgtcaccaa gcgttgagtt gtgtactcaa cagaagaatc ctgccaatgg
     3481 tcttcgatat cttggtacaa agttccaccc atggcaacgt taaagagttg ggtaccacgg
     3541 cagacagaga aaatgggctt tttctgttta atagcttcct tgatgagggc cagttcgaag
     3601 atatctcttt gaaggtgata gtcatcgcta tcaatggttt tgggttcgcc atagaatttt
     3661 ggatcgacat tttgcccacc tgtcaagatg agcttgtcaa tcaaactgat atagtggcag
     3721 gccatttctt gatcaccaat cggtaggatg atgggaatcc ctccagcatc tttaacgcct
     3781 tcaacaaagc cttttgctgc gtagctcatc atgatgtcat catctggatg agttttttcg
     3841 tttcctgtaa tcccaataac tggttttttc ataaaatgat tttcgctttc taatcctctt
     3901 ttcgcatgaa gtagaggagg gtttggagtt cacttgtcaa atcgacatac tgaacgacca
     3961 cgtcttttgg taaatgcaga tggactggtg aaaaactgag aattcctttc acaccagcat
     4021 caaccaagag attagcaacc tcttgtgact tgacgctggg aacagttagg atagcagtct
     4081 tcacatcagc atccttgatt ttatccttga tctgagaaat tccgtaaatg ggaatcccgt
     4141 caggagtttg ggtaccgact tcaggatggt catctaggtc aaaggccatg ataatcttca
     4201 tcttgttacg ttcgtggaag cggtagtgga gaagggcatg gcccatattt ccaataccaa
     4261 ccagcatgac attggtaata gagttgtcat tgagcaaatc ggcaaaaaat gtcattagtt
     4321 ttttgacatc atatccaaaa ccacgacgac caagttcacc aaaataggaa aaatcacgac
     4381 gtacggtcgc tgaatcaata ccgatagcct ctgcaatttg cttagagttg gcacgttcaa
     4441 tcttttctgc atgaaatctc ttaaaaattc gatagtagag agagagtctt tttgctgtag
     4501 cttttggaat agcaaactgt ttatctttca caaaatcaca acctttctat tcttctattt
     4561 tatagaaaca ttgtgaaaaa atcaacaaaa ataagaaaaa actaagaaaa atcttagttt
     4621 tgatgtaaaa aatctgcatg agatagaaaa cggtagaggt ctccgaccag accctgataa
     4681 actttttggc ccctaaaagt cagagaagtc acataaagtg tatctggtaa ggttacacat
     4741 cctgacaaag tcaacatgag agcctcatga tcctcatact tgagagtacg ctctacatga
     4801 tagcagtcct tataggtcag ttcaaacatt ttggctctat ctttccgatt ttgtaaagac
     4861 accacgttct accaagctat ccatgaggaa gtagaatttt tcctgatgaa tatggtggtc
     4921 ttctgatttg aaaatatcaa ctagacgaag gccaaactta tcagtgatat tgattttagc
     4981 ccctgtaact tccttgttaa tgatgatttt gagttggaag ccttcaccgc tgtttggcac
     5041 tttttccaaa aggcgagtca gttcatagtt accaacctta gtttcaaaaa aggtgttatc
     5101 tttgagggtg aattttttaa cagaagggct aagagtgtaa tcgtaacgac aattttttaa
     5161 ctgaatgatt ttttcaaatg ccatatggct aacctccgat aatttctttt aaggtttttg
     5221 cgagggtttg taggtcttca acggtatttt gtggcgacaa actgatgcga agggattcct
     5281 tcaagcgttc tgaatttgcg ccatacatgg cttcaagaac atggctggat tggacaacgc
     5341 ctgcagtaca ggctgagcca gtagagattg aaattccagc taaatctagc cgaaggagta
     5401 agaggtcatt tttctgacca ggaaatccaa tattgagaac ataagggaga tgatgttttc
     5461 ctctattcaa gtaatactga atgccctcca gctctgccag aaaggcagtt tctagatttt
     5521 gtacatgttg aaaatgttct tcttgttttt ctaggtcttc ttttagggct gcaaccatgc
     5581 ctacgatagc aggcagattt tcagttcctg cacgtttttt ctgttcctgg tctccgccat
     5641 gtagatagga atcaaagtcc atgctagatg cgtagagaaa accgattccc ttaggaccat
     5701 ggaatttgtg ggcagaagca gtgagaaaat caatgcccaa ttcttctgaa tgaattggga
     5761 ttttaccaat agcctgaact gcatcaacat gataggcagc agggtgttgc ttgagtattt
     5821 ggccaatttc agcgatgggc agtaggtttc ctgtctcatt attggtaaac atggtagaaa
     5881 ccaaaatcgt atcgtcacgt aaagcctttt gaatttgctg ggctgtgatt tcttgatttt
     5941 ctggctggat aatggttgct tcaaacccaa agtgttgaac caagtaatca attgtttcaa
     6001 ggacagcatg gtgctcgatg gcagttgtga tgatatgttt tccttgttct tggtgacgaa
     6061 gacagtagcc aatgatggta gtattattgc cttcagtccc accagaagtg aaaaagatat
     6121 gttgaggttt tgtccttagt aactgggcta gttcctgacg ggcttctcgc aagagtttgc
     6181 cagcttgacg accatgacca tgaatactag aaggatttcc gtgggtttct tgcataacct
     6241 tggtcatagc tgaaatagca actgctgaca taggagtcgt tgcagcattg tccaaataaa
     6301 tcaaagaatc accttatttc tttttattgt aggcaaagag tgggctgact ggttttcttt
     6361 cgtgaatacg tacgatagca tcaccaatta actcactagc agtgatatag catacatttt
     6421 taggagtttt ttcttttgtt gctactgaat cagtcacaag aatttcttta atattagtat
     6481 tgtcaagaag ctcagcagct ccctcgacga agagaccgtg gctagaaaca gcataaattt
     6541 ctgtagctcc ttcacgttca acgattttag aagcttcaga gaaggtacgt cctgtattta
     6601 aaatatcatc aatcaagata gctttcttac cttcaacatc accaataata taaccttcgt
     6661 tacgagttgc atcgtcttga gggtagtcga taatggcgat aggagcatca agatattcag
     6721 ccaggctacg cgcacgtttg acacctgaat ttttagggct aacgacaaca acatctgaac
     6781 caagcaatcc tttatcgcag taatgttttg cgaatagggg aacagtgaaa agattatcca
     6841 ctggaatatc aaagaaacct tgaacctgaa cggcatgcaa atcaagagtc aggatacgat
     6901 caactccagc cttaaccagc atattggcaa ctagttttgc tgtaagtggc tcacgaggac
     6961 aagcaatgcg gtcttgacgt gcatagccaa aatatggaag gacaacgttg atactgtggg
     7021 cacttgcacg cacacaagca tcgaccatga ttaacaattc cattaggtgg ttgttgacag
     7081 ggaaacttgt tgattggatg atgtaaacat cataaccacg gacactttct tcgatattta
     7141 cttggatttc tccgtctgaa aattgacgtg atgatagttt tccaagtggg acaccaacag
     7201 cttgggcaat tttttgtgca atctcttggt tagagttgag tgcgaaaagt ttcatgtttt
     7261 ttctatctga cattatagac cgtcctctgt aaactttata aatcctagtt atatttacct
     7321 tacatatatg aactgggatt tgtgtatttt tatcttttct attttaccaa aaaatggaga
     7381 ttatttcagc tatttttcat acttttgaca aatcgaacca attttgaagg agctttttgg
     7441 taggaaatct gatttttctc taaaaattgt cggaaatcct gtttgccttg ctcatgattt
     7501 tccacttcaa gctccaattc gtaatctgtt atatcaaagt atcggctctg atccagtgcc
     7561 atgagaccaa tagctgtttt catttcatag cgaagcgttg ttagacaacc aagaacctgc
     7621 cagttcttac tttggatacc atgtttcgcc aattcatcca gtactagtcc ttgaggaagt
     7681 tcttccttac tcagatagtc ctcagcatct tttagttgca atttttggtt gtattccatg
     7741 tttccaacac tctgcgggac tttgagtgtc aactcagccc agtcttcaaa ggttcgaatg
     7801 cgcatagcga ctttcttttc tcgcagttca aaatcaggcg tgtcgatgta gtaatttgtt
     7861 tgaagaacag gagtgacacc tgtgaactgg tcttttagac gattgtattc atcttttttc
     7921 aatagtgttt tcaattcaat ttctaaatgt ttcatttttc ttaccttttt ttatcgttga
     7981 aagcggattt atggtataat aagcattgta tttattgtat atgaatctgg agaaaaaatc
     8041 aaagatattt ttgacggata atatgagaac aagggagaat atatgacctt agaatgggaa
     8101 gaatttctag atccttacat tcaagctgtt ggtgagttaa agattaaact tcgtggtatt
     8161 cgtaagcaat atcgtaagca aaataagcat tctccaattg agtttgtgac cggtcgagtc
     8221 aagccaattg agagcatcaa agaaaaaatg gctcgtcgtg gcattactta tgcgaccttg
     8281 gaacacgatt tgcaggatat tgctggctta cgtgtgatgg ttcagtttgt agatgacgtc
     8341 aaggaagtag tggatatttt gcacaagcgt caggatatgc gaatcataca ggagcgagat
     8401 tacattactc atagaaaagc atcaggctat cgttcctatc atgtggtagt agaatatacg
     8461 gttgatacca tcaatggagc taagactatt ttggcagaaa ttcaaattcg tactttggcc
     8521 atgaatttct gggcaacgat agaacattct ctcaactaca agtaccaagg ggatttccca
     8581 gatgagatta agaagcgact ggaaattaca gctagaatcg cccatcagtt ggatgaagaa
     8641 atgggtgaaa ttcgtgatga tatccaagaa gcccaggcac tttttgatcc tttgagtaga
     8701 aaattaaatg acggtgtagg aaacagtgac gatacagatg aagaatacag gtaaacgaat
     8761 tgatctgata gccaatagaa aaccgcagag tcaaagggtt ttgtatgaat tgcgagatcg
     8821 tttgaagaga aatcagttta tactcaatga taccaatccg gatattgtca tttccattgg
     8881 cggggatggt atgctcttgt cggcctttca taagtacgaa aatcagcttg acaaggtccg
     8941 ctttatcggt cttcatactg gacatttggg cttctataca gattatcgtg attttgagtt
     9001 ggacaagcta gtgactaatt tgcagctaga tactggggca agggtttctt accctgttct
     9061 gaatgtgaag gtctttcttg aaaatggtga agttaagatt ttcagagcac tcaacgaagc
     9121 cagcatccgc aggtctgatc gaaccatggt ggcagatatt gtaataaatg gtgttccctt
     9181 tgaacgtttt cgtggagacg ggctaacagt ttcgacaccg actggtagta ctgcctataa
     9241 caagtctctt ggcggtgctg ttttacaccc taccattgaa gctttgcaat taacggaaat
     9301 tgccagcctt aataatcgtg tttatcgaac actgggctct tccattattg tgcctaagaa
     9361 ggataagatt gaacttattc caacaagaaa cgattatcat actatttcgg ttgacaatag
     9421 cgtttattct ttccgtaata ttgagcgtat tgagtatcaa atcgaccatc ataagattca
     9481 ctttgtcgcg actcctagcc ataccagttt ctggaaccgt gttaaggatg cctttatcgg
     9541 tgaggtggat gaatgaggtt tgaatttatc gcagatgaac atgtcaaggt taagaccttt
     9601 ttaaaaaagc acgaggtttc taagggattg ctggctaaga ttaagtttcg aggtggagct
     9661 attctggtca ataatcaacc gcaaaatgca acgtatctat tggacgttgg agactacgtt
     9721 accatttaca ttcccgctga ggaaggcttt gaaaccttgg aggctattga gcatccatta
     9781 gatattctct atgaggatga ccactttcta gtcttgaata aaccctatgg agtggcttct
     9841 attcctagtg tcaatcactc taacaccatt gccaatttta tcaagggtta ctatgtcaag
     9901 caaaattatg aaaatcagca ggttcacatt gttaccagac tagataggga cacttctggc
     9961 ttgatgctct ttgccaagca cggttatgcc catgcacgat tagacaagca gttgcagaag
    10021 aaatctatcg agaaacgcta ctttgctttg gttaagggag atggacattt ggagccagaa
    10081 ggggaaatta ttgctccgat tgcgcgtgat gaagattcca ttattaccag acgagtggct
    10141 aaaggcggaa agtatgccca tacttcatac aagattgtag cttcttatgg aaatattcac
    10201 ttggtctata ttcacctgca cactggtcga acccatcaaa tccgagtcca tttttctcat
    10261 atcggttttc ctttgctggg agatgatttg tatggtggta gtctggaaga tggtattcaa
    10321 cgtcaggctc tgcattgcca ttacctatcc ttttatcatc catttttaga gcaagacttg
    10381 cagttagaaa gtcccttgcc ggatgatttc agtaacctta ttacccagtt atcaactaat
    10441 actctataaa aactgtctca gagtataatt attatcttaa aggagaaaac tcatggaagt
    10501 ttttgaaagt ctcaaagcca accttgttgg taaaaatgct cgtatcgttc tccctgaagg
    10561 ggaagagcct cgtattcttc aagcaacaaa acgcttagta aaagaaacag aagtgattcc
    10621 tgttttgctt ggaaatcctg aaaaaattaa aatttatctt gaaattgaag gaatcatgga
    10681 tggttatgag gtcatcgacc ctcaacatta tcctcaattt aaagaaatgg tttctgcctt
    10741 ggtggagcgt cgcaagggca aaatgactga agaagatgta cgcaaggttt tggttgaaga
    10801 tgtcaactac tttggtgtga tgttggttta cttgggcttg gttgatggaa tggtgtcagg
    10861 agcgattcac tcaacagctt caacagttcg tccagctcta caaatcatca aaactcgtcc
    10921 aaatgtaact cgtacttcag gagccttcct catggttcgt ggtacggaac gttacctatt
    10981 tggagactgt gccattaaca tcaatccaga tgcagaagcc ttggctgaaa ttgccatcaa
    11041 ctcagcaatc acagctaaga tgtttggcat cgaacctaaa attgccatgt tgagctattc
    11101 tactaaaggt tcagggtttg gtgaaagcgt tgataaggtc gttgaagcaa ctaaaattgc
    11161 tcacgacttg cgtcctgacc ttgaaatcga tggtgagttg caatttgatg cagcctttgt
    11221 tcctgaaact gcagctctga aagctcctgg aagtacggta gctggtcaag caaatgtctt
    11281 catcttccca ggtatcgagg ccggaaatat cggttacaag atggctgaac gcctgggtgg
    11341 ctttgcggct gtaggacctg ttttgcaagg tttaaacaag ccagttaatg atctttctcg
    11401 tggatgtaat gcagatgatg tttacaagtt gaccctcatc acagcagctc aagcagttca
    11461 tcaatagtga aaactataaa gcgatatact atgctatact gtagttatga aactatgtac
    11521 gaaaagcact gccattaatt cctgagaact aaattactga ttggtgtcaa aaaggaaaac
    11581 ttccaagcga tgatatcctg tctatacacg acctatagaa atctgtaata tgtatgtccg
    11641 taaaacgata atttcccttt ttgattttaa atgagtatga aaagagaatt ttttcatgag
    11701 aaaacgcata atattaagtg tcttgacacc tgctattatg cgttttctgg ctctttgtca
    11761 actgtagtgg gttgaagaaa agctaagctc gagaaaggac aaattttgtc ctttcttttt
    11821 tgatattcag agcggtaaaa atccgttttt tgaagttttc aaagttccga aaaccaaagg
    11881 cattgcgctt gataagtttg atgagattat tggtcgcttc caatttggcg tttgaatagg
    11941 gtagttgaag ggcgttgacg attttctctt tgtcctttag aaaggtttta aagacagtct
    12001 gaaaaagagg atgaacctgc tttagattgt cctcaatgag tccgaaaaat ttctccggtt
    12061 ccttattctg aaagtgaaac agcaagagtt gatagagctg atagtgatgt ttcaagtctt
    12121 gtgaatagct caaaagcttg tctaaaatct ctttattggt taa
//
LOCUS       DAVBDG010000054        12009 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBDG010000054 DAVBDG010000000
VERSION     DAVBDG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.055"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(330..764)
                     /locus_tag="VQ526_001599"
                     /pseudo
     CDS             complement(330..764)
                     /locus_tag="VQ526_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(901..1785)
                     /locus_tag="VQ526_001600"
     CDS             complement(901..1785)
                     /locus_tag="VQ526_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2555303.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLAFQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTYVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(1789..2175)
                     /locus_tag="VQ526_001601"
     CDS             complement(1789..2175)
                     /locus_tag="VQ526_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2555304.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(2168..3511)
                     /locus_tag="VQ526_001602"
     CDS             complement(2168..3511)
                     /locus_tag="VQ526_001602"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2555305.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(3593..4477)
                     /locus_tag="VQ526_001603"
     CDS             complement(3593..4477)
                     /locus_tag="VQ526_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555306.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFEREIASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(4489..5106)
                     /gene="cysE"
                     /locus_tag="VQ526_001604"
     CDS             complement(4489..5106)
                     /gene="cysE"
                     /locus_tag="VQ526_001604"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2555307.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(5122..7335)
                     /gene="pnp"
                     /locus_tag="VQ526_001605"
     CDS             complement(5122..7335)
                     /gene="pnp"
                     /locus_tag="VQ526_001605"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2555308.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELITFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDSRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            complement(7894..8760)
                     /gene="metF"
                     /locus_tag="VQ526_001606"
     CDS             complement(7894..8760)
                     /gene="metF"
                     /locus_tag="VQ526_001606"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2555309.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIVDLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEEHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8824..11073)
                     /gene="metE"
                     /locus_tag="VQ526_001607"
     CDS             complement(8824..11073)
                     /gene="metE"
                     /locus_tag="VQ526_001607"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2555310.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYFFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(11333..11599)
                     /locus_tag="VQ526_001608"
     CDS             complement(11333..11599)
                     /locus_tag="VQ526_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2555311.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARNKQGISQKKLEEL
                     SGVSQPVIARMETGKTSPQLDTVLKVLASLGKILAVVRLEQGKS"
     gene            complement(11725..12009)
                     /locus_tag="VQ526_001609"
                     /pseudo
     CDS             complement(11725..12009)
                     /locus_tag="VQ526_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3360 a         2706 c         2327 g         3616 t
ORIGIN      
        1 tagtgactat acttttcata tttataattc attcctttcg tttcactcaa ggcacaacac
       61 agaatgaaaa agtgttgtga tctttatttt gttttataat aatagtgaga aagcctatca
      121 ctactacaaa tcacggggag gtgaataagt gagtggtaca gccactacct cgcatatttt
      181 gtcacatcat ttaacggtac ataataagtt gtaccatctg aataagttgc tacaatatca
      241 tttgcatgct ctccttcacc tttagcaaag gttggagctc ctgctggatg atttttattt
      301 gcctctttca atttttcaat aatggcattt ttttctgtat cttttatatt atcaggattt
      361 ttcactaaaa ttttgtctgg atatgtcggt ttagcagaaa caatttttac tgttacttct
      421 tttttattcg aagcacttgt ccagtttcca gcattatctt tagcatttaa ttttacagta
      481 attcctgaac taggaacttc agtagcaggt tgattatcaa cattattcaa ctttaatttc
      541 aaaagagctg ttgcatcaga cgttttatca atcgttatat ataatgatga attgttatta
      601 taaccagttc cttcatattt agctgtttgt gagctacttg aaacagaact gaaattatac
      661 ccactacctc cctgattatc ttcaatgctt acgtctaaat aaacttcccc actattattt
      721 agcttagcaa caactgttat agtaaaataa cataaaattc acataaatag attagggaaa
      781 tcaaagcaac ttctaggaat gttttagcag tcacagtgta ctttcccagc atcaagccac
      841 tataactctg cacataaaaa tggagaagat ggccatcctc ttctccaaat attaacttct
      901 ttacaaacca actatagttg acaaagaacc taaaatcaat tgataacaca aggtcaggtc
      961 ggtcaactct ttcaactgaa gccctgtcaa ctcttcccat ttatcaatct tgtattggag
     1021 agaattgcgg tgcagataga gttgctgggc tgttttagtg agaacagcac tattttccca
     1081 aagagagaga atgatttcct gaatctgatc ttgatccaaa atcatctggt gtagacattc
     1141 cttgattggc ttcaagtcca cgagtctttc tcccatactc caaagataga gctgagaaaa
     1201 agtatgaaca ccttggtgac cctgacgcca ccatgtcttg aacaaatccc gctcagcttt
     1261 gattaagtct gatagggctt gatgtcccgt ctgagaccaa acctgaccca acatgataga
     1321 aagacgaagt ccaaagtcat actcaaccgc ttcaatcgta tcacttaaaa tatctcttac
     1381 ataagtgtat ttgtcttgtt gaagcacgaa aacataatcc tgagatccga cctgtagcac
     1441 tgtctgacaa ttcggaaaaa gagtccgcat catatctagc caagaagcca gattttcctg
     1501 ctgaaaataa gaaagatggc aataaaccaa ctgaatcttt ttaaaaactt gcggtgcctg
     1561 tcccttgcct tcaaccagat aggaatacca agggtttagc gaacgagcct gctcctgctg
     1621 ggtcaaaagg gcaaccaact gcttttcacg ctcgctgagc ccagcttcct ccagcaaaat
     1681 ccactgctga aaagctaaag ggagcgtgag atagccctct ttctctactg gttggtctga
     1741 aatccgagcc tcaggaaacc agtcttgtag ttctttttcc ctcatgttct agccctccac
     1801 tttttggatg caccatgaaa ccaaactctc aagacgttcc agattctcag tcatatggag
     1861 atagcccata accgcttcaa atcccgtgga catacgataa gtcacgacat ctgcattttt
     1921 agcctttgtg tggctattgg tattgcggcc acgtttgtag atttcttctt ctttttccgt
     1981 taggacctgc tcctccaaca tgagagcaat caggcgagcc tgagccttgg ctgacacata
     2041 cttggttgct tcttgatgga gtttattggg tttggtcata cctttgagga tgaggtgacg
     2101 gcgaatatac atagaataca ccgcatcccc ctcaaaggct agcgcaatcc cgttaatgag
     2161 attgacatca atcacgtgtc cacctcactc catccttggt atcaaggagc ttaattcctt
     2221 gagtaaccaa ttggtcacgg atttggtctg ctgtctcaaa gtctcgattg gcacgcgcct
     2281 cttggcgttt ttgaatcaag tcttcaatct ctgcatccaa aacttcctca acaaagacaa
     2341 ttccaaaaat ttctaacata tctgcaagag cttgcttgac acttgcatca tagttccctg
     2401 agttgatcca tttggccatt tcaaagacaa ctgtgatacc gttggcagca ttaaaatctt
     2461 catccatagc tgctacaaac ttatctttaa agttttgtaa ctcttgggca tccacgtttc
     2521 ctgtaaatgg ttgttcgtaa gtattcttca gatacttgag attggtctcg gcatcgcgaa
     2581 ctgccttttc cgtgaagttg ataggcttac ggtagtgctg ggtcgcaaag aagaaacgaa
     2641 gtacttgccc atcaagagtt ttaagggcat cgtgtaccgt aatgaagtta cccaaggact
     2701 tagacatttt gacattgtcg atattgacaa agccattgtg catccagtag ttagcaaaag
     2761 ccttgcctgt tttagcttca gattgggcaa tttcattggt gtggtgtgga aactctaggt
     2821 cagctccacc accgtggata tcaatggtat cacctaaaat ctctgtcgac atgactgaac
     2881 actcaatatg ccaacccgga cgtccaggtc cccaaggact atcccaagaa atctcacctg
     2941 gtttggaaga tttccataga gcaaagtcta caggattttc cttacgagcc gtttcttcat
     3001 cggtacgacc tgaagcacct agctccaaat cttccaaggt tttattagcc aatttagcat
     3061 agttgtggga tttttctaca cggaaataga catccccttg actctcatag gcaaagcctt
     3121 tctcgatcaa gtcttccaca aaacggatga tgtctgccat aaactccact acacgcggat
     3181 ggcgagtcgc aggtttcacg cccaaggccg tcacatcctc acgaaaggca gcgatgtact
     3241 tatccgcaac ctcctgaggc gtgatgcctt cttccctggc acggttgata atcttatcat
     3301 ccacatctgt aaaattggaa atataggcaa ccttataccc acggtactca aaatagcgac
     3361 gaatcgtatc aaaagctacc gtcgaacggg cgtttcccac gtggatatag ttgtacaccg
     3421 ttggcccaca aacatacatc ttgatcttgc cgtcctcaat cgggacaaat tctcgcaaat
     3481 cacgagacat ggtgtcatag attttaatca taaatcataa tcaggaaagc tgaaatccaa
     3541 gaacaattag tttcatcact aaaagttcaa gtaaatttca gtccgaatat ctctacactt
     3601 cggaatccct tgctcctttc tcattcagat aaaccacctg agtctgtttg acaaagccaa
     3661 ttttttcata caaacgtttg gcacctacat tgctatcttc cactgcaatc tgaaattcct
     3721 tgtcattttg ctcaattagt tggttgacga gggattttgc taagtagctt ccatagcctt
     3781 ttccacgttc aggttccaat attgctaaac cgtagaagta attcgtatta gtcgataaat
     3841 caaccgtaca agtcccaata acctgaccgt cttttaataa aatatataag cgactttctg
     3901 gatccttcag agcttcagcg acatatctat ccacaacttc tctcgattca tgttcctctg
     3961 aaaatgcctg aaattttaat tgactaattt gatcctgata cgaactatct gctaacaaaa
     4021 cttcaagatg ggaaacattt gctaacggat aaggtcttct atccttacct aaccaagttt
     4081 ctgtctcttc atcctcgatt agtccccagt tactggcaaa gtcaggatga ttctctaaaa
     4141 aaatacgttc tgtctgaaaa gtgactgacc gaatggggaa agaagctatt tctctctcaa
     4201 aactagtaaa caatgcacgc gcaatcccct gacggcgatg acctggatga accagtatcg
     4261 ttacttctac atcttggtca tctgcataga cagttaataa accaacaagt tcgccttttt
     4321 cataataaag gaaaaaggcg ggcatgtttg ggtcaaaatt aagcatgtta gagagatagg
     4381 gatcgcgata ggtaccgtca tagttttggc aacagttaat tacttttttc gcttcagata
     4441 gctcctcttg gcttaacttg tttcttgctt gaatcatata ggtatcctct acaaaccaga
     4501 cgatctgtga ctggcatctt tagcctgctc gagtttattg acataatact ctcgtttttc
     4561 ttcgacttcg tgaatgacag gctcatcttt cttaccatga agacggacaa tcttggccgg
     4621 aataccgaca accgtcacgt cactaggtac atctgctacg acaactgctg cagcaccgac
     4681 cttggcattt tcaccaattt ccacaggccc gataacttgg gcatgggctg atatgagggc
     4741 tccctttcgt acagtcggat ggcgtttgcc acagtctttc cctgttcccc cgagagtcac
     4801 tccgtgatag agaagaacgc ctttttcaac aatcgctgtc tctccaatca ccagaccaga
     4861 accatggtca ataaaaacac ctgaatcaat ctgggctcct ggatgaatct caatctgagt
     4921 ccaaaagcgc caaaactgac tgtacatacg agctaatagt ttgaagccgt gcttccagag
     4981 aaaatgcgag agacggtggg ccgccaaggc cttgacacct ggataagtca gcaaaacctc
     5041 caaagtggtg cgggccgctg gatcattttc ttttacaata tcaatggttt cgcgccacca
     5101 ccccatacat ttctcctttt cttattctga atcttttggt gtttctgtaa attctttctt
     5161 aggtttgtga tccttttgat gacgtgggcg gtgagggcgc tcagactttt cacctttttc
     5221 atcatgctca ggttttggcg gacgaggtag aagagccttc atagaggcat cgatacggcc
     5281 tttttcatca atcttgataa ccttaacatc aacttcatcc ccgatttcta ccaaatcctc
     5341 tacacgattg gtacgagtcc aagccatctc agagatatga acaagggcat ctgtcttatc
     5401 aaagaggtta acaaaggcac caaatttctc gatacgaacg actttagcac ggtaaacttc
     5461 atccactttg gcttcacgaa ccaaaccagc aataatttct ttggcacggt taatagcatc
     5521 ttggtcacta gagtagatag acacatttcc ttcttcgtct atatcaatct taacacctgt
     5581 ttcagcgata atcttgtcga tggtttctcc acccttaccg atgacaatct taatcttgtc
     5641 cacatcaatc ttgatcgtat caattttcgg agcagttgga gccaattctg gacgaacttc
     5701 tggaatggtt gcttcaatga catcaaggat ttcaaaacgc gctttcttgg cttgagcaag
     5761 agcctccgtc aagatttctg cagtaatccc ttgaatcttg atatccattt gaagggctgt
     5821 aatcccatca cgagtacctg caaccttgaa gtccatatct ccaaagtgat cttccaaacc
     5881 ttggatatct gtcaatactg tgtagttatt tccatctgag ataagtccca tagcaatacc
     5941 agctactggc gccttgattg gcacaccacc agccataagg gcaagagttc ccgcacagat
     6001 agaagcttga gatgaagaac cgtttgattc caaaacttct gctactagac ggatagcgta
     6061 tgggaattct tccaagcttg gcaagacttg agcaagagca cgctcaccaa gagcaccgtg
     6121 accgatttca cgacgacctg gcgcaccgta acgacctgtt tcccctacag aatattgagg
     6181 gaagttatag tggtgcataa agcgtttctt gtactctgga tccaaaccat caatgatttg
     6241 agtttctccc atcggagcca aggtcaagac tgaaagagct tgagtttgcc cacgagtaaa
     6301 gagacctgaa ccatgtacac gaggaaggaa gtcaacaacc gcatccaaag gacggatttc
     6361 atcgacctta cgactatcag gacgcacctt gtcttctgta attaaacgtc gcacttctgc
     6421 gtgttccatt tgttccaaga tttcagccac atcacgcata atacggtcaa attcttcgtg
     6481 gttcgcatat ttttcttcgt aaacggcagt cacttggtct ttcactgctt gagttgcagc
     6541 ttcacgggcc aatttctctt ctacttgaac tgccttttgg aggtcactgt tgtaggctgc
     6601 aatgatttca gcttgcaatt cagcatccac gtgaagcaat tccacttctg ctttttcttt
     6661 accgacagca gcaacgattt cttcttggaa ggtaatcaat tctttgacag cttcgtgccc
     6721 tttaagaagc gcttccaaca tgatttcttc tgacaattct ttggcaccag actctaccat
     6781 gttgatagcg tgcttggttc cagctactgt caattcaaga agagattgct ctgcttgttc
     6841 ttgacttggg ttgatgatga tttggccatc tacatatccc acttgtaccc cagcaattgg
     6901 tccgtcaaat ggaatatctg aaatagacag tgccaaagat gaaccaaaca tagcagccat
     6961 tggtgcagat gcattttcat cataagaaag cactgtattg atgacttgga cttcattacg
     7021 gaaaccttcc gcaaacatag gacgaatcgg acggtcaatc aaacgcgctg tcaaggtcgc
     7081 atctgttgaa ggacgtcctt cacgtttcat aaagccacca ggaaacttcc cagccgcata
     7141 cattttttct tcgtagttga cttggagtgg gaagaaatcc ccagttgcca ttttcttaga
     7201 cataacggca gcagtcaaga cagttgactc accgtaacgt acgacaacag atccatttgc
     7261 ttgcttagca acctgaccag tctctacaat taactcacga cccgcaaaag tcgtttgaaa
     7321 cacttgtttt gccattttaa tccccttttg attgatgaaa ttatacgcct tgcctacaaa
     7381 gatcaagata ccaaggacgt caaaagcaaa gtaaaaatag gaaactgacg aagtcttcga
     7441 tgaagacaag acagtttatc ttttttacac agcttttcgg ccgtgttcaa ttactcaaga
     7501 taccaagccg tcaagcaact caaaggaaga taggaaatcg aacgacggag cgactactcc
     7561 tagggagatt tatctttttc cacagagttg taggcaagtt cagttttcaa gatacatcat
     7621 tagaaaggtt taatactaaa gtatctaaag ctttcacgct aatcgctatc gggcgattag
     7681 ctaaatgctt tactaactct ctcgtcaaat aacatcgatt tgactcactc gtgtcgttaa
     7741 atcttacagt ttaaatgcat tgtattattt aataccttca tctttgtatc aagtacgtac
     7801 agaatttatt ttatcatatt tttcttaaaa agtgaggtct ttaccattaa aaaggaacca
     7861 ttcccctcac ctgagaagaa tggtttgctt ttattatcct agagactggt gattaaacaa
     7921 ggcatgggtt gcttgatgga tgtattttgc tgtatcagca ttattcatcg tatagagatg
     7981 cacaccggca acatcctgag ttaccaagtc cacgatttgg tccactgcat aggcaagtcc
     8041 tgctgctctg agcgactcag ggtcatgctc atacttgtct aagatggctt taaatttgcg
     8101 tggaagatgg atattctcac aagtcttcaa gagtcggaga gcctgatttc gattcagaat
     8161 tggcataatt cctgcatgaa tgggaacatc aatcccagcc aagatacact tgtcctgaaa
     8221 atcatagaag cgctcattgt caaagaagag ctgagttacg aggctcgaac agcctgcatc
     8281 cactttcttc ttaagatttt gaatatctga aatctgattt ggcgaatctg gatgctcttc
     8341 tggatagcaa gctccaataa tatcaaagtg aggggcttgt tccttgataa actcaatcaa
     8401 gtcggttgca tagcggaaat ccttttgtgg ttctacgtct ggaataatat ccccacgaag
     8461 agccaagatt ttctgaaccc caactttgtc caagtcaaca atagtttcag caaccttgtc
     8521 cttagttaga taaatagctg gcaagtgggc aatggtcgga atcgccaagt cattttggat
     8581 aaagtcagcc aaacgaaccg tcgtttcctt gatattaaat ttattattgc tggcagttac
     8641 actgataaaa tggggagcca actcctgcat atcctgcaag gctgaaataa tgttaccatt
     8701 acccacggct gggtttggag ggaacacttc aaatgagagt gacggtgttt ggcgtgacat
     8761 atgtaataac cttttctagt tgatttcttt ttgaacaacc actgtatgga gagaaatcca
     8821 atcttacaat ttctcacgcg cagctttagc tgcttcaaca aggcggatca agctttcttt
     8881 tgtttctggg ataccacgtg ttttcaaacc acagtcaggg ttgatccaaa ctttcttgct
     8941 tggcacttta gcaaggatgg cttcgattgt gttgtcgatt tcgccttcgt ttggtacacg
     9001 aggtgagtgg atatcgtaaa ccccaggtcc cacttctgtt tggaagtttt tcgctttgag
     9061 ttcgtccaag atttcaaggt ttgaacggct ggcttcaaag gaaataacgt ctgcatccat
     9121 gttatcgata gctgggatga tatctgtaaa ttctgagtaa cacatgtgag tgtggatttg
     9181 tgtgtctgac gctactgttg agtgtaccaa gcggaaggca ggaattgccc agtcaaggta
     9241 atcttcgtac cagtcactac gacggagtgg caatttttca cgaagagcag cctcatcaat
     9301 ttggatgatt ttcacaccag cagcttcaag gtcaagcact tcatccttga tagcaagggc
     9361 gatttgaaga gtagaatcct tgatagagat gtcttcacgt gggaatgacc agttaaggat
     9421 ggtaacaggt ccagtcaaca tacctttaac aggtttgttt gtacgacttt gtgcatagct
     9481 agaccattta acagtgatag ggttaagacg agtgacatca ccccagatga ttggtggttt
     9541 taccccacgc ataccgtatg attgtaccca tccattttta gagaagaagt atcctgacaa
     9601 gttttgaccg aagtactcaa ccatgtcatt acgctcaaat tcaccgtgaa caaggacatc
     9661 aaagtcaata tcttcttgcc acttgatcca ttcgtcaatc gtttcagcaa ggaaagcatc
     9721 gtactctttt tgagacaatt cacctttacg gtaagccaaa cgtttggcac gaacttcttt
     9781 tgtttgaggg aatgaaccaa tcgttgttgt tggaagagct ggaagtttga aagcttcttc
     9841 ttggatagct tcacgttctg caaaggctgg caaacgagtg tagtctgcgt ctgtcaagcc
     9901 agcgatacgc gcacgaagtt cagcattttc accaacacgc tcagtcgcaa agagttcttt
     9961 gttggctgca agagcttctg aaccttgacc attgcggata gcatccaagt cacggatttc
    10021 atccaatttt tcaactgcaa aggcaaagtg gttcaagagt gctggttcaa attcttcatt
    10081 agcagttgta aatggcacat gaagaagtga gcaagagctt gtcaaaacga tgttttcagc
    10141 tgggatttgc tcaagaacag ccaagctctt ttcgtagttg ttgcgccaga tgtttttacc
    10201 attgacaata cctacataga gagtcttgtc agctgggaag ccacctttaa cgagttcaag
    10261 agttttctta ccttcaacaa agtcaagacc gatagcatct actggcaagt ttacaaggtc
    10321 agcgtatacg tcacgaacat caccgaaata agtttgaagc aagacttcaa gacctttttt
    10381 gtcagccaag agtttgttgt aaaggttcaa gaagagagct ttttcttcag ctgtcaagtc
    10441 ttttacaaga gccgcttcat ccaattggat gcgagtcgca ccaagttcag ccaatttagc
    10501 aaaaacttct tggtaagcag ccactaagct atctacgaag tcgtctgctt tcacgccttc
    10561 ttcaaagtct gacaattgaa ggaaagtgaa tggacctaca agaacaggac gagtgttcaa
    10621 tccaagttct tttgcttctt ggaactcatc gaaaatcttg tgaccagcca attttacttg
    10681 agtgtctttt tcaaatttag gaacgatgta gtggtagtta gtgttgaacc atttcttcat
    10741 tggaagggcg cgaacgtccc ctttttctcc ctggtaacca cgtcccaaag cgaagtagcg
    10801 ctcaaggtca gacaagtcca agttttgaac ggatgcaggc accacgttga aaaggaaagc
    10861 cgcatctagg aagttatcat agtgagaaaa gtcatttgat ggaatttcag tgatgccttt
    10921 ttctttgaca atgttccagt gtttagcacg caagtctttt gctgctgcta aaagttcttc
    10981 ttctgagatt tcttttctaa agtatttttc agttgtaaat tttaattcac ggaattcgcc
    11041 caaacgaggg aaaccgatga ttgtagttga catgatgtgt cctccaaaat ttgttgttga
    11101 aactatctta acagaaaaga aagcatctgt ataattgtaa aaaattaggc tttgatatag
    11161 tttgaaacta tacctctatt ttagacaaaa gaaaaagact tgagacacat gcctcaaatc
    11221 ccttttataa cttgttttta gctgtatttt agttagaatg ctcaaacgcg ttattagtaa
    11281 ttcttataag tgactatggc ttgttattag aaaagactat aactgattct agtcaacttt
    11341 tcccctgttc aagtcggacg actgctagta tctttcctag actagctagg acttttaaga
    11401 ctgtgtccaa ctgtggacta gtcttacctg tctccatcct agctataaca ggctgactca
    11461 ctccactgag ttcctctagc tttttctgac tgattccttg cttatttctg gcttcaatca
    11521 actcgctcat gatagctact cgcatatcac tttcaaggat ttcctccttg ctaaagagct
    11581 cagatggaca tccttccaat tactcccaat agcactattc ttcatcactt aaccctcttt
    11641 cttttacgtc tatgtatttt taaaaaaatg agcgaattat gattcgatag attgaccagc
    11701 gggtttaaag ttggtgctag cctatttctt aagcgatttt ccttttctag gataaagcag
    11761 atcctgcttg cttaacccca attttccacg atgaatccaa tagtaaatgg ttgaaattcc
    11821 cacgttaacc cctttagcca taaccatcat ttcaggcgaa aatttttggt tatgtatagt
    11881 ggagaatctt ttcctttagt tccttggtca agcttgattt cttgaccgag cgcttgcgat
    11941 tgttttcata agactgttga gcgtagtcgg cagaataaac ctctttgaag cgcccttttc
    12001 caagacatt
//
LOCUS       DAVBDG010000055        11807 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBDG010000055 DAVBDG010000000
VERSION     DAVBDG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.056"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            389..616
                     /locus_tag="VQ526_001610"
     CDS             389..616
                     /locus_tag="VQ526_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555312.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            904..3786
                     /gene="nanA"
                     /locus_tag="VQ526_001611"
     CDS             904..3786
                     /gene="nanA"
                     /locus_tag="VQ526_001611"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2555313.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QALANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGVIG
                     LEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKGSIESM
                     HNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLVTLTTK
                     EDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            4801..5253
                     /locus_tag="VQ526_001612"
     CDS             4801..5253
                     /locus_tag="VQ526_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2555314.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            5272..6609
                     /locus_tag="VQ526_001613"
     CDS             5272..6609
                     /locus_tag="VQ526_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2555315.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            6688..7572
                     /locus_tag="VQ526_001614"
     CDS             6688..7572
                     /locus_tag="VQ526_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2555316.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            7572..8405
                     /locus_tag="VQ526_001615"
     CDS             7572..8405
                     /locus_tag="VQ526_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2555317.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            8423..10516
                     /gene="nanB"
                     /locus_tag="VQ526_001616"
     CDS             8423..10516
                     /gene="nanB"
                     /locus_tag="VQ526_001616"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2555318.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            10528..11631
                     /locus_tag="VQ526_001617"
     CDS             10528..11631
                     /locus_tag="VQ526_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2555319.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
BASE COUNT         3984 a         1848 c         2450 g         3525 t
ORIGIN      
        1 gtgttttgag ttgactttgt cagtcttatc tacagcctca aaacagtgtt ttgagcatca
       61 tgcggctagc ttcttagttt gctctttgat tttcattgag tataaaaaca gatgagtttc
      121 tgttttcttt ttatggacta taaatgttca gctgaaacta ctttcaagga cattattata
      181 taaaagaatt ttttgaaact aaaatctact atattacact atattgaaag cgttttaaaa
      241 atgaggtata ataaatttac taacgcttat aaaaagtgat agaatctatc tttatgtata
      301 tttaaagata gattgctgta aaaatagtag tagctatgcg aaataacaga tagagagaag
      361 ggattgaagc ttagaaaagg ggaataatat gatatttaag gcattcaaga caaaaaagca
      421 gagaaaaaga caagttgaac tacttttgac agtttttttc gacagttttc tgattgattt
      481 atttcttcac ttatttggga ttgtcccctt taagctggat aagattctga ttgtgagctt
      541 gattatattt cccattattt ctacaagtat ttatgcttat gaaaagctat ttgaaaaagt
      601 gttcgataag gattgagcag gaagtatggt ataaatagca taagctgatg tccatcattt
      661 gcttataaag agatatttta gtttaattgc agcggtgtcc tggtagataa actagattgg
      721 caggagtctg attggagaaa ggagagggga aaattggcac caatttgaga tagtttgttt
      781 agttcatttt tgtcatttaa atgaactgta gtaaaagaaa gttaataaaa gacaaactaa
      841 gtgcattttc tggagtaaat gtcttatttc agaaatcggg atatagatat agagaggatc
      901 agtatgaatc ggagtgttca agaacgtaag tgtcgttata gcattaggaa actatcggta
      961 ggagcggttt ctatgattgt aggagcagtg gtatttggaa cgtctcctgt tttagctcaa
     1021 gaaggggcaa gtgagcaagc tctggcaaat gaaactcaac tttcggggga gagctcaacc
     1081 ctaactgata cagaaaagag ccagccttct tcagagactg aactttctgg caataagcaa
     1141 gaacaagaaa ggaaagataa gcaagaagaa aaaattccaa gagattacta tgcacgagat
     1201 ttggaaaatg tcgaaacagt gatagaaaaa gaagatgttg aaaccaatgc ttcaaatggt
     1261 cagagagttg atttatcaag tgaactagat aaactaaaga aacttgaaaa cgcaacagtt
     1321 cacatggagt ttaagccaga tgccaaggcc ccagcattct ataatctctt ttctgtgtca
     1381 agtgctacta aaaaagatga gtacttcact atggcagttt acaataatac tgctactcta
     1441 gaggggcgtg gttcggatgg gaaacagttt tacaataatt acaacgatgc acccttaaaa
     1501 gttaaaccag gtcagtggaa ttctgtgact ttcacagttg aaaaaccgac agcagaacta
     1561 cctaaaggcc gagtgcgcct ctacgtaaac ggggtattat ctcgaacaag tctgagatct
     1621 ggcaatttca ttaaagatat gccagatgta acgcatgtgc aaatcggagc aaccaagcgt
     1681 gccaacaata cggtttgggg gtcaaatcta cagattcgga atctcactgt gtataatcgt
     1741 gctttaacac cagaagaggt acaaaaacgt agtcaacttt ttaaacgctc agatttagaa
     1801 aaaaaactac ctgaaggagc ggctttaaca gagaaaacgg acatattcga aagcgggcgt
     1861 aacggtaaac caaataaaga tggaatcaag agttatcgta ttccagcact tctcaagaca
     1921 gataaaggaa ctttgatcgc aggtgcagat gaacgccgtc tccattcgag tgactggggt
     1981 gatatcggta tggtcatcag acgtagtgaa gataatggta aaacttgggg tgaccgagta
     2041 accattacca acttacgtga caatccaaaa gcttctgacc catcgatcgg ttcaccagtg
     2101 aatatcgata tggtgttggt tcaagatcct gaaaccaaac gaatcttttc tatctatgac
     2161 atgttcccag aagggaaggg aatctttgga atgtcttcac aaaaagaaga agcctacaaa
     2221 aaaatcgatg gaaaaaccta tcaaatcctc tatcgtgaag gagaaaaggg agcttatacc
     2281 attcgagaaa atggtactgt ctatacacca gatggtaagg cgacagacta tcgcgttgtt
     2341 gtagatcctg ttaaaccagc ctatagcgac aagggtgatc tatacaaggg taaccaatta
     2401 ctaggcaata tctacttcac aacaaacaaa acttctccat ttagaattgc caaggatagc
     2461 tatctatgga tgtcctacag tgatgacgac gggaagacat ggtcagcgcc tcaagatatt
     2521 actccgatgg tcaaagccga ttggatgaaa ttcttgggtg taggtcctgg aacaggaatt
     2581 gtacttcgga atgggcctca caagggacgg attttgatac cggtttatac gactaataat
     2641 gtatctcact taaatggctc gcaatcttct cgtgtcatct attcagatga tcatggaaaa
     2701 acttggcatg ctggagaagc ggtcaacgat aaccgtcagg tagacggtca aaagatccac
     2761 tcttctacga tgaacaatga acgtgcgcaa aatacagaat caacggtggt acaactaaac
     2821 aatggagatg ttaaactctt tatgcgtggt ttgactggag atcttcaggt tgctacaagt
     2881 aaagacggag gagtgacttg ggagaaggat atcaaacgtt atccacaggt taaagatgtc
     2941 tatgttcaaa tgtctgctat ccatacgatg cacgaaggaa aagaatacat catcctcagt
     3001 aatgcaggtg gaccgaaacg tgaaaatggg atggtccact tggcacgtgt cgaagaaaat
     3061 ggtgagttga cttggctcaa acacaatcca attcaaaaag gagagtttgc ctataattcg
     3121 ctccaagaat taggaaatgg ggagtatggt atcttgtatg aacatactga aaaaggacaa
     3181 aatgcctata ccctatcatt tagaaaattt aattgggact ttttgagcaa agatctgatt
     3241 tctcctaccg aagcgaaagt gaagcgaact agagagatgg gcaaaggagt tattggcttg
     3301 gagttcgact cagaagtatt ggtcaacaag gctccaaccc ttcaattggc aaatggtaaa
     3361 acagcgactt tcctaactca gtatgatagc aagaccttgt tgtttgcagt agataaggaa
     3421 gatatcggac aggaaattat tggtatagct aaaggaagca tcgaaagtat gcataatctt
     3481 cctgtaaatc tagcaggtgc cagagttcct ggcggagtaa atggtagcaa agcagcggtg
     3541 catgaagttc cagaatttac agggggagtt aatggtacag agccagctgt tcatgaaatc
     3601 gcagagtata agggatctga ttcgcttgta actcttacta caaaagaaga ttatacttac
     3661 aaagctcctc ttgctcagca ggcacttcct gaaacaggaa acaaggagag tgacctccta
     3721 gcttcactag gactaacagc tttcttcctt ggtctgttta cgctagggaa aaagagagaa
     3781 caataagaga agaattctaa acatttgatt ttgtaaaaat agaaggagat agcaggtttt
     3841 caagcctgct atcttttttt gatgacattc aggctgatac gaaatcataa gaggtctgaa
     3901 actactttca gagtagtctg ttctataaaa tatagtagat tgaaataaga tgtgaacaac
     3961 tctatcagga aagtcaaatt aatttataga aaaattttag cagtcaaggt gtactgttat
     4021 agattcaata tattatgtga ctattaacct tctcttctcc taaaattgac tttcttgttt
     4081 tcttatcttg tccactcgaa acaagtattg taagaatttg attatttttg aaagtacttt
     4141 taatatactt gatatagtta aaaaagattt gaaactaaat tccaaattag aaaaagactt
     4201 gaaatactaa aaaaaaaaag tatactctaa ttgaaaacgg taacaaaact aatttagaga
     4261 atgaaatata gagtatttct ctcttaaaag tttttggtga aacgagatgt agaaaggaga
     4321 tttagccaaa gagtctatta gtgctagaat aatagattag aattatttta gaaaaacgaa
     4381 gtgagcagct tataaattca agtccccaaa tagattcata ctagtatctt ttgcaaaaaa
     4441 taaagggcga cttccttcat gaatatcaat ttcatctata aggaaggtag ctaattgaac
     4501 taacttattt attctgtttg tcgctagaaa aatcagacct ccttgtgaag attgaggaga
     4561 tacttaatga aaatcaaaga agaagctagc aagctagtag cagattgccc aaaacaccgc
     4621 tttgaggttg tagataagac tgacctatat aatccaaggt gaagcgactg tggtttgaag
     4681 agattttcaa agagtatagg ctagagagta gtgtttttat gtccttctag tagaaaatgc
     4741 tagacagaag aatggggaac ttggatagga aaaatagatt gagaaaggag gttagaagag
     4801 atgattatta caaaaattag ccgtttagga acttatgtgg gagtcaatcc acattttgca
     4861 acattaatag attttctaga aaaaacagga ctagaaaatt taacagaagg ttcgattgct
     4921 atcgatggta atcgattgtt tgggaattgc tttacttatc tagcagatgg tcaagcaggg
     4981 gctttctttg aaacacacca aaaatatttg gatattcatt tagttttgga aaacgaagaa
     5041 gccatggctg ttacatcgcc ggaaaatgta agcgttaccc aagaatatga tgaagagaaa
     5101 gatattgaat tatacacagg gaaagtggaa cagttggttc agttgagagc tggcgaatgc
     5161 ctcatcactt ttccagaaga tttacatcaa cccaaggttc gtataaatga tgaacctgtg
     5221 aaaaaagttg tctttaaagt tgcgatttct taatgtagaa agagaagaac gatgaaaaaa
     5281 atgagaaagt ttttatgtct agctggaatt gcgctagcgg ctgttgcctt ggtagcttgt
     5341 tcaggaaaaa aagaagctac aactagtact gaaccaccaa cagaattatc tggtgagatt
     5401 acaatgtggc actcctttac tcaaggaccc cgtttagaaa gtattcaaaa atcagcagat
     5461 gcttttatgc aaaagcatcc aaaaacgaaa atcaagattg aaacattttc ttggaatgac
     5521 ttctatacta aatggactac aggtttagca aatggaaatg tgccagatat cagtacagct
     5581 cttcctaacc aagtaatgga aatggtcaac tcagatgctt tggttccgct aaatgattct
     5641 atcaagcgta ttggacaaga taaatttaac gaaactgcct taaatgaagc aaaaatcgga
     5701 gatgattact actctgttcc tctttattca catgcacaag tcatgtgggt tagaacagat
     5761 ttgttaaaag aacataatat tgaggttcct aaaacttggg atcaactcta tgaagcttct
     5821 aaaaaattga aagaagctgg agtttatggc ttgtctgttc cgtttggaac aaatgactta
     5881 atggcaacac gtttcttgaa cttctacgta cgtagtggtg gaggaagcct cttaacaaaa
     5941 gatcttaaag cagacttgac aagccaactt gctcaagatg gtattaaata ctgggttaaa
     6001 ttgtataaag aaatctcacc tcaagattct ttgaacttta atgtccttca acaagctacc
     6061 ttgttctatc aaggaaaaac agcatttgac tttaactctg gcttccatat cggaggaatt
     6121 aatgccaaca gtcctcaatt gattgattcg attgatgctt atcctattcc aaaaatcaaa
     6181 gagtctgata aagaccaagg aattgaaacc tcaaacattc caatggttgt ttggaaaaat
     6241 tcaaaacatc cagaagttgc taaagcattc ttagaagcac tttataatga agaagactac
     6301 gttaaattcc ttgattcaac tccagtaggt atgttgccaa ctattaaggg gattagcgat
     6361 tctgcagcct ataaagaaaa tgaaactcgt aagaaattta aacatgctga agaagtaatt
     6421 actgaagctg ttaaaaaagg tactgctatt ggttatgaaa atggaccaag tgtacaagct
     6481 ggtatgttga ctaaccaaca cattattgaa caaatgttcc aagatatcat tacaaatgga
     6541 acagatccta tgaaagcagc aaaagaagca gaaaaacaat taaacgattt atttgaggct
     6601 gttcagtaga tgtaaaagac tagaaaatag gtgggctagt gagctgaaaa gctctagccc
     6661 aatcttgtaa aagaagggag aaggagaatg gttaaagaac gtaatttaac tcgctggata
     6721 tttgttttgc cagctatgat tatcgtagga ttactctttg tttatccgtt tttctcgagt
     6781 attttttata gctttaccaa taagcatttg attatgccta attataaatt tgttggtttg
     6841 gctaactata aagctgtgct atcagatccc aacttcttta atgcgttctt taattcaatt
     6901 aagtggaccg ttttctcatt agttggtcaa gttttagtag ggtttgtatt ggctttagct
     6961 cttcacagag tacgccactt caagaaatta tataggacct tattgattgt tccttgggca
     7021 tttcctacca tcgttattgc cttctcttgg cagtggattc taaacggggt ttatggctac
     7081 ttacctaatc taatcgtaaa attaggttta atggaacata cacctgcatt tttgacagat
     7141 agtacatggg cattcctatg tttggtgttt atcaacattt ggtttggagc accaatgatt
     7201 atggttaatg tgctttcagc tttgcaaaca gtaccagaag aacaatttga ggctgctaag
     7261 atagatggtg cttcaagttg gcaggtgttc aagtttatcg tctttccaca tattaaagtg
     7321 gttgtaggac ttctagttgt tttgagaact gtatggatct ttaataactt tgacattatc
     7381 tacctcatta ctggtggtgg accagccaat gctacaacga cgcttccaat ttttgcttac
     7441 aacctgggtt ggggaactaa attgttgggt cgtgcttcag cagttacagt attgctcttt
     7501 atcttcttgg tggcgatttg ctttatctac tttgctatca tcagtaagtg ggaaaaggag
     7561 ggtagaaaat aatgaagaag aaatccagta tttatttaga tattctctca catgtacttt
     7621 tagttggtgc gaccatcgtt gcaattttcc cattggtatg gattatcata tcttctgtca
     7681 aagggaaagg ggaattaact cagtatccaa cacgattttg gcctgaacag tttacattag
     7741 attatttcac tcatgttatc aacgatttgc acttcattga taacattcga aacagtttaa
     7801 tcattgcctt ggctacaacc ctcattgcga ttattatttc tgctatggca gcctatggta
     7861 ttgttcgatt ctttcctaaa ttgggagcaa tcatgtcgag actactcgtc attacctaca
     7921 ttttcccacc aattttgtta gcaattccct attcaattgc cattgctaaa gttgggttaa
     7981 caaatagttt atttggcttg atgatggttt atctatcttt tagtgttcca tatgcagttt
     8041 ggctcttagt tggatttttc caaacagttc caattggaat tgaagaagcg gctagaattg
     8101 atggtgcaaa taaatttgtt acgttttata aagttgtgct accgattgta gcaccaggta
     8161 ttgtagcaac agctatttat acatttatca atgcttggaa tgaattcctg tatgccttga
     8221 ttttgattaa caatacagga aagatgacag tagcagtagc ccttcgttca cttaatggtt
     8281 cagaaatact agactgggga gatatgatgg cagcgtctgt tattgtagtt cttccatcaa
     8341 ttattttctt ctctatcatc caaaataaga ttgcaagtgg attatcagaa ggatctgtga
     8401 agtagacgaa agaaggaaaa aaatgaataa aagaggtctt tattcaaaac taggaatttc
     8461 tgttgtaggc attagtcttt taatgggagt ccccactttg attcatgcga atgaattaaa
     8521 ctatggtcaa ctgtccatat ctcctatttt tcaaggaggc tcatatcaac tgaacaataa
     8581 gagtatagat atcagccctt tgttattaga taaattgtct ggagagagtc agacagtagt
     8641 aatgaaattt aaagcagatg aaccaaactc tcttcaagct ttgtttggcc tatctaatag
     8701 taaagcaggc tttaaaaata attacttttc aattttcatg agagattctg gtgagatagg
     8761 tgtagaaata agagacgccc aaaagggaat aaattattta ttttccagac cagcttcatt
     8821 atggggaaaa cataaaggac aggcagttga aaatacacta gtatttgtat ctgattctaa
     8881 agataaaaca tacacaatgt atgttaatgg aatagaagtg ttctctgaaa cagttgatac
     8941 atttttgcca atttcaaata taaatggtat agataaggca acactaggag ctgttaatcg
     9001 tgaaggtaag gaacattacc tcgcaaaagg aagtattggt gaaatcagtc tatttaacaa
     9061 agcaattagt gatcaggaag tttcaactat tcccttatca aatccatttc agttaatttt
     9121 ccaatcagga gattctactc aagctaacta ttttagaata ccgacactat atacattaag
     9181 tagtggaaga gttctatcaa gtattgatgc acgttatggt gggactcatg attctaaaag
     9241 taagattaat attgccactt cttatagtga tgataatggg aaaacgtgga gtgagccaat
     9301 ttttgctatg aagtttaatg actatgagga gcagttagtt tactggccac gagataataa
     9361 attaaagaat agtcaaatta gtggaagtgc ttcattcata gattcatcca ttgttgaaga
     9421 taaaaaatct gggaaaacga tattactagc tgatgttatg cctgcgggta ttggaaataa
     9481 taatgcaaat aaagccgact caggttttaa agaaataaat ggtcattatt atttaaaact
     9541 aaagaagaat ggagataacg atttccgtta tacagttaga gaaaatggtg tcgtttatga
     9601 tgaaacaact aataaaccta caaattatac tataaatgat aagtatgaag ttttggaggg
     9661 aggaaagtct ttaacagtcg aacaatattc ggttgatttt gatagtggct ctttaagaga
     9721 aaaacataat ggaaaacagg tccctatgaa tgttttctac aaagattcgt tatttaaagt
     9781 gactcctact aattatatag caatgacaac tagtcagaat agaggagaga gttgggaaca
     9841 atttaagttg ttgcctccgt tcttaggaga aaaacataat ggaacttact tatgtcccgg
     9901 acaaggttta gcattaaaat caagtaacag attgattttt gcaacatata ctagtggaga
     9961 actaacctat ctcatttctg atgatagtgg tcaaacatgg aagaaatcct cagcttcaat
    10021 tccgtttaaa aatgcaacag cagaagcaca aatggttgaa ctgagagatg gtgtgattag
    10081 aacattcttt agaaccacta caggtaagat agcttatatg actagtagag attctggaga
    10141 aacatggtcg gaagtttcgt atattgatgg aatccaacaa acttcatatg gcacacaagt
    10201 atctgcaatt aaatactctc aattaattga tggaaaagaa gcagtcattt tgagtacacc
    10261 aaattctaga agtggccgta agggaggtca attagttgtc ggtttagtca ataaagaaga
    10321 tgatagtatt gattggaaat accactatga tattgatttg ccttcgtatg gttatgccta
    10381 ttctgcgatt acagaattgc caaatcatca cataggtgta ctgtttgaaa aatatgattc
    10441 gtggtcgaga aatgaattgc atttaagcaa tgtagttcag tatatagatt tggaaattaa
    10501 tgatttaaca aaataaagga gaaaaacatg gttaaatacg gtgttgttgg agcagggtat
    10561 tttggagctg aattggctcg ctacatgcaa aagaatgatg gagcagagat tactcttctc
    10621 tatgatccag ataatgcaga ggcgattgca gaagaattgg gagcaaaagt agcaagttcc
    10681 ttagatgagt tggtttctag cgatgaagta gattgtgtta tcgtcgcaac tccaaataat
    10741 cttcataagg aaccggttat taaggctgca cagcatggta aaaatgtttt ctgtgaaaaa
    10801 ccaattgcgc tttcttatca agattgtcgc gagatggtag atgcgtgtaa agaaaacaat
    10861 gtaaccttta tggcaggaca tattatgaat ttctttaatg gtgttcatca tgcaaaagaa
    10921 ctcattaatc aaggagttat cggagacgtt ctatattgtc atacagctcg taatggttgg
    10981 gaagaacaac aaccgtcagt atcatggaaa aaaattcgtg aaaaatcagg tggtcacttg
    11041 tatcaccaca tccatgaatt ggattgcgtt caattcctta tggggggcat gcctgaaact
    11101 gtaaccatga caggtggaaa tgtggcccat gaaggtgaac atttcggtga tgaagatgat
    11161 atgatttttg tcaatatgga attttctaat aagcgttttg ctttgttaga atggggttca
    11221 gcttatcgtt ggggtgaaca ttatgtctta atccaaggaa gcaaaggtgc catccgctta
    11281 gacttattca actgtaaagg aactcttaag ctagatgggc aagaaagcta tttcttgatt
    11341 cacgaatcgc aagaagaaga tgatgatcgg actcgtatct atcatagtac agagatggat
    11401 ggagcaattg cttatggtaa accaggtaaa cgtactccat tatggctatc atctgtcatt
    11461 gataaagaaa tgcgctatct gcatgagatt atggaaggag ctccagtatc agaagaattt
    11521 gcaaaacttt tgacaggtga agctgcccta gaagcaattg ctactgcaga tgcttgtacc
    11581 cagtctatgt ttgaagatcg caaagtaaaa ttgtcagaaa ttgtaaaata aattttggta
    11641 ttctcctatt tataggtcga cttgctcctc tgaaagtact tttagaggag ctgtttgact
    11701 ttgctagttt ttgaaactga aatctattat actacaaact attgaaagcg ttttaatttt
    11761 aaggtataat aatctcatag aaataaagaa aaggaggaaa gaggatg
//
LOCUS       DAVBDG010000056        11491 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBDG010000056 DAVBDG010000000
VERSION     DAVBDG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.057"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(300..1280)
                     /gene="dusB"
                     /locus_tag="VQ526_001618"
     CDS             complement(300..1280)
                     /gene="dusB"
                     /locus_tag="VQ526_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2555320.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1267..2139)
                     /gene="hslO"
                     /locus_tag="VQ526_001619"
     CDS             complement(1267..2139)
                     /gene="hslO"
                     /locus_tag="VQ526_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2555321.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2304..3731
                     /locus_tag="VQ526_001620"
     CDS             2304..3731
                     /locus_tag="VQ526_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2555322.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3890..5398
                     /gene="glpK"
                     /locus_tag="VQ526_001621"
     CDS             3890..5398
                     /gene="glpK"
                     /locus_tag="VQ526_001621"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2555323.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFDSGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQMDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5440..7266
                     /gene="glpO"
                     /locus_tag="VQ526_001622"
     CDS             5440..7266
                     /gene="glpO"
                     /locus_tag="VQ526_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2555324.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7336..8040
                     /locus_tag="VQ526_001623"
     CDS             7336..8040
                     /locus_tag="VQ526_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2555325.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(8634..9242)
                     /locus_tag="VQ526_001624"
     CDS             complement(8634..9242)
                     /locus_tag="VQ526_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555326.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            10333..>11491
                     /gene="ltrA"
                     /locus_tag="VQ526_001625"
     CDS             10333..>11491
                     /gene="ltrA"
                     /locus_tag="VQ526_001625"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2555327.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKFLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRHKTLVGTPQGGNLSPLLSNVMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSASRFIEKRLGLKVNMTKAKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     RIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHY"
BASE COUNT         3389 a         2239 c         2633 g         3230 t
ORIGIN      
        1 aggtgccctt gcagtcaaca caactgtaga tggctatgga gtcaatgcca atggtgaatg
       61 ggtaaactaa acctaatata actagttaat actgacttcc tgtaagaact ctttaaagta
      121 ttccctacaa ataccatatc ctttcagtag ataatatacc cttgtaggaa gtttagatta
      181 aaaaataact ctgtaatctc tagccggatt tatagcgcta gagactacgg agtttttttg
      241 atgaggaaag aatggcggca ttcaagaggc tctttaagag agttacgggt tttaaactat
      301 taagccttct ccaattgcaa gagggcttca atctctgcta gggtgctagc ttgcgaaatg
      361 gctccacgga gtttggcagc gccagatgtt ccacggagat agtgaggagc gaggccgcgg
      421 aattcacgaa ctgcgacgtt ttctcctttg aggttaatca atcgtttcaa gtgttcgtag
      481 gcgatcttca tcttgtcttc aaatgtcaaa tcaggtagga tttctcctgt ttcaaagtaa
      541 tggttgattt ggttgaagag gtaaggattt cccatggcag ctcggccaat catgactgcg
      601 tcagcaccaa cttcttcgat gcgttgcttg gcttcttgga cagtacggat atcaccgttg
      661 gcgatgaatg gaatcttggt tagagcttgg gcaaccttgt aaagggtctc aaggtccgcg
      721 tggccagtat acatttgttc acgggtacgg ccatgcatgg cgagggcaga aacacctgca
      781 gcctcagcag cgagggcatt ttctactgca agagatgggt ccgcccagcc ggtacgcatt
      841 ttgacagtaa gtgggatatc aaggacagac tggaccttgt tgatgataga gtagatcttg
      901 tcaggatcct tgagccacat agcaccagct tcgttcttca cgattttgtt gacagggcag
      961 cccatgttga tatcgacgat atcggtcttg gtgttttctt ggatgaattc tgctgcgcgt
     1021 gctaggctgt cttcatcgct accaaaaagt tggatagaga cagggttttc gccctcatcg
     1081 atatgaagca tgtgcagggt tttttcgttg ttgtattgga ttcccttgtc agagaccatt
     1141 tccattacaa cgagtccagc tccgagctct tttgctatgg tacgaaaggc tgagttggtc
     1201 acgccagcca taggcgctaa aacggtacga ttgggaatct caatattgcc aatcataaaa
     1261 ggtgtattaa gatttgtcac gaatgagttc ctccaggtcc ttttcatcaa agttgtaagt
     1321 agtttggcag aattgacaag tgatttctgc accgtggtct tcctctttca tttcctgtaa
     1381 gtctgagctt ggaaggctgg caagagcgtt cataaagcgt tcatggctac agtcacattg
     1441 gaaacggatt tcttcttcag aaagacgctt gtaggcttcg tccccgtaga tagccttgag
     1501 gagggcttcg atatggtcgt cgctttcgag aagagtagag atagctggca tttcttggat
     1561 gcgtttttca aagcgagcaa tctcttcttt cttggctcct ggcaagactt gaactaggaa
     1621 accacctgca accttgacct tgtcttcctc gtccaaaagg acattgaggc cgaccgctga
     1681 aggcgtttgt tggctttcag taaggtaaaa ggcaaggtct tcaccgattt ctccagagat
     1741 gaggggagtt atagagttgt aaggatttcc agtaccgtag tctgtgataa cgaggaattg
     1801 accatttcca acaaaaggtc cgactaggac ttcaccagtc gcagtctttt tgatgtcaac
     1861 accaggattt tgaacatagc ctttgacgtt ccccttggta tcagcgacgg tgataatagc
     1921 acctagagag ctagatccca acaccttaac tgtaagtttg gtatttcctt tttcattggc
     1981 tgcgagaatc tggctggcga taagagttcg accaagcgct acagttgagc tagcttgggt
     2041 ttgatgtttt tcttgagcag tgcggacggt ttcagtgcta tcaaggacaa aagcacgaaa
     2101 ggctccgctt tctgatatag ttttaataat tttatccata gctactattt tagcataaaa
     2161 atgcccaaag ggggagccgt gtgtttactg attttcagga taatggacca ggaaatcagc
     2221 atgaaaataa aaagagaaac agattatttt agcatttgta agatttatgc tatgcttaag
     2281 gtagaaaatg aaagggataa caaatgtatt taggagattt gatggagaaa gccgagtgtg
     2341 gtcaattttc aatcctttcc tttctattac aagagtctca gacgaccgtc aaggctgtaa
     2401 tggaagaaac aggattttca aaagcaaccc taaccaaata tgtcaccctg ctcaatgaca
     2461 aggctttgga tagtggctta gaactgacta ttcactcaga agatgaaaat ctgcgtctgt
     2521 ctatcggtgc agctaccaag gggagagata ttcggagctt gtttttggag agtgctgtta
     2581 aataccagat tttggtttat cttctctacc accaacagtt tttagcccat cagctggctc
     2641 aagaattagt gattagcgag gctacgcttg gtcgtcactt ggctggttta aatcagattt
     2701 tgtcagaatt tgatttatcc atccaaaatg gacgttggcg aggtccagag catcagattc
     2761 gctatttcta tttctgtctt ttccgaaagg tctggtcgag tcaggaatgg gaaggtcaca
     2821 tgcagaaacc agagagaaaa caggagattg ccaatttaga ggaaatctgc ggtgcaagtt
     2881 tgtctgcggg gcagaaattg gacttggttc tctgggctca catcagtcaa caacgtcttc
     2941 gggtcaatgc ttgtcagttt caagttatag aagagaaaat gcgagggtat tttgacaata
     3001 tcttctatct tcgtttgctg agaaaggttc cgtccttttt tgctgggcaa catattccac
     3061 taggagttga ggatggtgag atgatgatat tcttctcttt tctcctatct catcgcattc
     3121 ttcctcttca tactatggag tatattcttg gttttggagg gcagttggca gatttactga
     3181 cgcaattgat tcaagaaatg aagaaggagg aactattggg ggattataca gaggaccatg
     3241 tcacctatga actcagtcag ctttgtgctc aagtctatct ctataagggc tatattttac
     3301 aggatcgcta caagtaccag ttagagaatc gtcatccata tttactgatg gaacatgatt
     3361 ttaaagagac agcagaggag atttttcatg ctctacctgc ttttcaacag gggacagatt
     3421 tagataagaa gattctctgg gaatggctcc agttaatcga atatatggct gaaaacggtg
     3481 gccagcatat gcggattggt ctggatttga catctggttt tcttgtcttt tcaaggatgg
     3541 cagccatttt gaaacggtat ttggaataca atcgttttat taccattgaa gcttatgacc
     3601 ctagtcggca ttatgatttg ctggttacca ataaccctat tcataagaag gaacagacac
     3661 cagtctatta tttaaaaaat gacttggata tggaagattt ggcagggatt cgtcagttat
     3721 tattcactta aaaggcttgg ttaatccagg tcttttttgt gaaattcaca caatctcctc
     3781 acattttttt aaaaattaaa aaaagttgat aaacaagaaa gcgctttatt ttgtatacta
     3841 gtaagtgtaa agaggaaaca cctcaagatc tttatcagga ggacagtaca tgtcacaaga
     3901 aaaatacatc atggccattg accagggaac tacaagttct cgtgccatca ttttcaacaa
     3961 aaaaggggaa aaggttagct cgagtcaaaa agagtttacc cagattttcc ctcaagcagg
     4021 ttgggttgag cacaatgcca atgaaatttg gaactctgtt cagtcagtta ttgcgggtgc
     4081 tttcatcgaa agtggtgtca agccaaatca aatcgaggca atcgggatta ccaaccaacg
     4141 tgaaacaacg gttgtctggg ataagaaaac aggacttcct atctacaatg ctatcgtttg
     4201 gcagtcacgc cagacagcac ctttggctga gcaactaaaa agccaaggtt atgtggaaaa
     4261 attccatgaa aagactggtt tgattattga tgcttacttc tctgctacca aggttcgttg
     4321 gattttggat catgtagaag gtgctcaaga gcgagcagaa aaaggggaat tgctctttgg
     4381 tactatcgat acttggttgg tttggaaatt gactgacggt gcggctcacg tgactgacta
     4441 ctcaaatgca gctcgtacca tgctttataa cattaaagaa ctcaaatggg atgatgagat
     4501 tttggaaatc cttaacattc cgaaggctat acttccagaa gttcgttcta actccgaaat
     4561 ctacggcaag acagctccat tccatttcta cggtggagag gtgccaatct caggtatggc
     4621 tggggaccaa caagcagccc tctttggaca gttggctttt gattcaggta tggttaagaa
     4681 tacttatgga acaggctctt tcatcatcat gaatactggg gaagagatgc agttgtctga
     4741 aaacaacctc ttgacaacca ttggttacgg aatcaacggt aaggtttatt atgccttgga
     4801 aggttctatc ttcatcgcag gaagtgctat tcagtggctt cgtgacggtc ttcgcatggt
     4861 tgaaaattca ccagaatctg aaaaatacgc tcgtgattct cacaacaacg atgaagttta
     4921 tgtcgttcca gcctttacag gtctaggcgc tccatactgg aaccaaaatg ctcgtggttc
     4981 cgtctttggt ttgactcgtg gaacaagcaa agaagacttt atcaaggcga ctttgcaatc
     5041 tattgcttat caagtgcgtg acatcatcga caccatgcaa atggatactc agaccgccat
     5101 tcaagtactg aaggtggatg gtggtgcagc catgaacaac ttcctcatgc agttccaggc
     5161 ggatatttta ggcattgaca ttgcacgtgc taaaaacttg gaaacaacag ctctaggagc
     5221 ggccttccta gcaggtttgt cagtagggta ctggaaagac ttggacgagt tgaaactctt
     5281 gaacgagaca ggagaactct ttgagccatc tatgaacgaa tctcgcaagg aacaactcta
     5341 caagggctgg aagaaggctg tgaaagcaac tcaagtcttt gcggaagtag acgactaata
     5401 ctggcagaat aaagcgattt atttagaaag tgtgtaaata tggaattttc aaagaaaaca
     5461 cgtgaattgt caattaaaaa aatgcaggaa cgcaccctgg acctcttgat tatcggtgga
     5521 ggaatcacag gagctggtgt agccttgcag gcggcagcta gcggtcttga gactggtttg
     5581 attgaaatgc aagactttgc agaaggaaca tctagtcgtt caacaaaatt ggttcacgga
     5641 ggacttcgtt acctcaaaca atttgacgta gaggtggtct cagatacggt ttctgaacgt
     5701 gcagtggttc aacaaatcgc tccacacatt ccaaaaccag atccaatgct cttaccagtt
     5761 tacgatgaag atggagcaac ctttagcctc ttccgtctta aagtagccat ggacttgtac
     5821 gaccttttag caggtgtgag caacacacca actgcgaaca aggttttgag caaggatcaa
     5881 gtcttggaac gccagccaaa cttgaagaag gaaggcttgg taggaggtgg agtgtatctt
     5941 gacttccgta acaacgatgc gcgtctcgtg attgaaaaca tcaaacgtgc caaccaagac
     6001 ggtgccctca ttgccaacca cgtgaaggca gaaggcttcc tctttgacga aagtggcaag
     6061 attacaggtg ttgtagctcg tgatctcttg acagaccaag tatttgaaat caaggcccgt
     6121 ctggttatta atacaacagg tccttggagt gataaagtac gtaatttgtc taataaggga
     6181 acgcaattct cacaaatgcg cccaactaag ggagttcact tggtagtgga ttcaagcaaa
     6241 atcaaggttt cacagccagt ttactttgac acaggtttgg gtgacggtcg tatggtcttt
     6301 gttctcccac gtgaaaacaa gacttacttt ggtacaactg atacagacta cacaggtgat
     6361 ttggagcatc caaaagtaac tcaagaagat gtagattatc tacttggcat tgtcaacaac
     6421 cgctttccag aatccaacat caccattgat gatatcgaaa gcagctgggc aggtcttcgt
     6481 ccattgattg cagggaacag tgcctctgac tataatggtg gaaataacgg taccatcagt
     6541 gatgaaagct ttgacaactt gattgcgact gttgaatctt atctctccaa agaaaaaaca
     6601 cgtgaagatg ttgagtctgc tgtcagcaag cttgaaagta gcacatctga gaaatatttg
     6661 gatccatctg cagtttctcg tgggtctagc ttggaccgtg atgacaatgg tcttttgact
     6721 cttgctggcg gtaaaatcac agactaccgt aagatggctg aaggagctat ggagcgcgtg
     6781 gttgacatcc tcaaagcaga atttgaccgt agctttaaat tgatcaattc taaaacttac
     6841 cctgtttcag gtggagaatt gaacccagca aatgtggatt cagaaatcga agcctttgcg
     6901 caacttggag tatcacgtgg tttggatagc aaggaagctc actatctggc aaatctttac
     6961 ggttcaaatg caccgaaagt ctttgcactt gctcacagct tggaacaagc gccaggactc
     7021 agcttggcag atactttgtc ccttcactat gcaatgcgca acgagttggc tcttagccca
     7081 gttgacttcc ttcttcgtcg taccaaccat atgctcttta tgcgtgatag cttggatagc
     7141 atcgttgagc cagttttgga tgaaatggga cgattctatg actggacaga agaagaaaaa
     7201 gcaacttacc gtgctgatgt cgaagcagct ctcgctaaca acgatttagc agaattaaaa
     7261 aattaagaaa aaataaaaga ggtggagggc agcattcctt gtcgcccgtc ccttcttttt
     7321 aatggagaca gaaagatgat gaatgaatta tttggagaat ttctagggac tttaatcctg
     7381 attcttctag gaaatggtgt tgttgcaggt gtggttcttc ctaaaaccaa gagcaatagc
     7441 tcaggttgga ttgtgattac tatgggttgg gggattgcag ttgcggttgc agtctttgta
     7501 tctggcaagc tcagtccagc tcatttaaac ccagctgtga ccatcggtgt ggccttaaaa
     7561 ggtggtttgc cttgggcttc cgttttgcct tatatcttag cccagttcgc aggggccatg
     7621 ctgggtcaga ttttggtttg gttgcaattc aaacctcact atgaggcaga agaaaatgca
     7681 ggcaatatcc tggcaacctt cagtactgga ccagccatca aggatactgt atcaaacttg
     7741 attagcgaaa tccttggaac ctttgttttg gtgttgacaa tctttgcttt gggtctttac
     7801 gattttcagg caggtatcgg aacctttgca gtgggaactt tgattgtcgg tatcggtcta
     7861 tcactaggtg ggacaacagg ttatgccttg aacccagctc gtgaccttgg acctcgtatc
     7921 atgcacagca tcttgccaat tccaaacaag ggagacggag actggtctta cgcttggatt
     7981 cctgttgtag gccctgttat cggagcagcc ttggcagtgc ttgtattctc acttttctag
     8041 tttatactct tcgaaaatca aattcaaacc acgtcagcgt cgccttaccg tactcaagta
     8101 cagcttgcgg ctagcttcct agtttgctct ttgattttca ttgagtatta gaaaacaatt
     8161 atgttgatag agcttgggca agagcccaat ttcagcaaaa aatgaagtaa atcttctcat
     8221 aataaaacgc atcatatcaa gcacgaaaat tccacgaggt caactacagt cagaaagctg
     8281 aacaacaagc caaaacgccc aaaaaaggcg gcaaaaagca agcacctgca agcaacgtgc
     8341 cgaaatggtc aaatcctgat tatgtcaacg aattagaccc aaaaatcgtt gatatgctag
     8401 tagaatttca caagtcacaa gtcacaaggc actttggaaa ctcccgaggc gcaagcagaa
     8461 atcgcccaaa aacgtgaaga aatcgagcaa aggagagctg agcttgagga taaaaaacaa
     8521 gagcttttga accgcttgaa caaatagagt ttcgcaagta ttatgcttac aaattacttg
     8581 agcaattaac taaaatataa accctgcctt tatatctagg cagggtttat attttagaaa
     8641 ttcacgtagg ttgttacggt ttttacatac ccagtatagt ttgagtttct atagtattca
     8701 gtgataaact tccattttct ttgagcaaca tggatataag tacttgttat gtagtatgga
     8761 tatgggcttt gtgaatccaa gtaagactga taagcttgta taccaaaata tgctccacca
     8821 attattgcac cccatggacc ccccaataaa gcacctatcc taccaatcat ataactgatt
     8881 ccagcaccag tcatgaagtt agcgaatgtg ttagcttgtt tattcccatg tattgtgttg
     8941 acgtaattcc aaacattagg atcgtatgat ctaaaagata tatttaggtc gatttcattc
     9001 ttttgataag ccatataaaa tgccccattg atatagacgc cgtcagcacg tcgttcaata
     9061 gtgtctacac ttccatctgg attgacaacc tcaagaactt catcgcttaa aatatttact
     9121 tgcgtatctc cgaaccgcac tgatgagcca ttctcaaact gagcctcacc agatacaact
     9181 ttagagtttg ccgataagct atcatcagca aaaacaacaa gcgacgggga aatgctagac
     9241 atacagaaaa cagacataac tagcaaacac atgcatttaa acatcttaga cataacggaa
     9301 actcctttgt atttttgatt tttttcaact tttattatac aataaaacca aataaaaaga
     9361 aagcggtaac aatatgctta atgcgaaaat tttttatata tttttatgtt tgatcgttat
     9421 cgaaactaca ggcttgttgt tgttgaaaag aggtctcgaa atgggttatt tagacacaga
     9481 agctattatc ctcgcagttt tttcatttgc tttttacaac ctatgttcat tcgcttgggt
     9541 ctgctctaca ataaaaaaca ataaaaaata aatagacgta ttttcaaaaa aaaaaaaaaa
     9601 tgcatattta ttttagtaaa acgacgattt aaatcgtcgt ttttttgtag tacgacgggc
     9661 atgtcgtata tctgaggtgc aagtcctccg tgggcacttg ctaccggtga acccaaaagc
     9721 gattcccaag cctgactatc gtgaggtagc ggggagagga agagatagcg aaatcgtggc
     9781 tctacgaaca ggaacgtgat agcaaggcgt atatagcgga taaggaggct tcaaactcta
     9841 aagtccaaaa aggtagtcgt aacctatatg tgtaaatcac gagagtaatt gaattcggac
     9901 taaggtttgt gtgaaaaaga taaatctttc tagagtctaa agactctgcg tcagatttcc
     9961 tattttcact gtaacctttt aacgtcctca tatcttgtat aaacgaggaa agatgtacga
    10021 cttatcccgt gaggtttcat gagcgctgaa agcgtagtaa caacgaatca tgagaagtca
    10081 gccgagccca tagtagtgag gaaacttccg taatggaagt ggagcgaagg ggtgaatact
    10141 caaacagtct ggggagagac tgtttgaggt ctgtcgctag aaagagaaaa cgacagatcg
    10201 aagtaatcct acttcacttg tgtctgtaaa atgagtggtc tgatagaact ggactttgac
    10261 acgtaatgac tagatgaagg ttcaccaata taagatgtcc ctcaggcacc aaaacaagaa
    10321 aggaatacgc acatgtcaaa attgctagat aagatattat cacgcgaaaa tatgctggaa
    10381 gcctacaatc aagtaaaatc caataaaggc tcagctggga ttgatggaat gactatcgaa
    10441 gagatggata attatctcag acaaaactgg cgcttgacta aggaactgat aaaacagaga
    10501 aaatataagc ctcaaccagt tcttagagtt gagattccta aaccaaacgg aggtatccgt
    10561 caactaggaa ttccaacagt tatggataga atgattcaac aggccattgt ccaagttatt
    10621 agccccattt gtgaacccca tttctcagat acgagttatg gcttcagacc aaataggtca
    10681 tgtgaaaaag ccatcatgaa gttcttagag tacttaaatg acggctatga gtggatagtg
    10741 gatatagacc tagagaaatt tttcgatacg gttcctcaag atagattgat gtccttggta
    10801 cataacatta tcgaagacgg agatacggaa tccctgattc gtaagtatct tcattcaggt
    10861 gttatcatta atggtcagcg tcataaaacg ctagttggca cgccacaggg aggaaattta
    10921 tctcctctct tatccaatgt catgcttaat gaattggaca aggaattaga aaagagggga
    10981 cttcgatttg tgcgctacgc agatgattgt gtgattacgg tcggaagcga ggcagccgct
    11041 aagcgtgtga tgtattcagc cagtcgtttt attgagaagc gactaggttt gaaagtaaat
    11101 atgaccaagg ctaagattac cagaccaaga gagctgaaat acttaggttt tgggttctgg
    11161 aaatcatcag atggttggaa aagtcgtccc catcaagata gtgttcggag atttaagctt
    11221 aaattgaaga aactaacaca gaggaaatgg agtatagact taacaagacg tattgagcaa
    11281 ctgaatttgt ctattcgagg atggataaac tatttctcgc tgggaaatat gaagcgcata
    11341 gtcgctagca tagatgagcg cttgcgtact cgcctacgag tgattatctg gaagcaatgg
    11401 aagaagaaat cgagacgact atggggattg cttaagttag gagttcctaa atggatagca
    11461 gataaggtat ctggctgggg cgaccattat c
//
LOCUS       DAVBDG010000057        11364 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBDG010000057 DAVBDG010000000
VERSION     DAVBDG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.058"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            326..2308
                     /locus_tag="VQ526_001626"
     CDS             326..2308
                     /locus_tag="VQ526_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2555328.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVQFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2609..7912
                     /locus_tag="VQ526_001627"
     CDS             2609..7912
                     /locus_tag="VQ526_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HET2555329.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWKTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGNMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIETLKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALTKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASEQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(8079..10238)
                     /locus_tag="VQ526_001628"
     CDS             complement(8079..10238)
                     /locus_tag="VQ526_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET2555330.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQSAQP"
     gene            complement(10235..10831)
                     /gene="recU"
                     /locus_tag="VQ526_001629"
     CDS             complement(10235..10831)
                     /gene="recU"
                     /locus_tag="VQ526_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2555331.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKRQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10897..>11364
                     /locus_tag="VQ526_001630"
     CDS             10897..>11364
                     /locus_tag="VQ526_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 family protein"
                     /protein_id="HET2555332.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYF"
BASE COUNT         3514 a         2117 c         2533 g         3200 t
ORIGIN      
        1 attaaatagc gagaaatatc tatcctatct tctagaatgt cttccaaacg aggaaactct
       61 cgtaaacaaa gagattttag aggcctattt accgtggact aaagttgtac aagaaaagtg
      121 caaataagaa atctccagat taggaactat ccgtgagttc tctagtctgg agatttttca
      181 atagacttcg ttattgggcg gttacgatat tcatattttt tgcaaagatg ttgtttgaaa
      241 aataattttc aaaaattctg aaaattctgt tgacatcttt ctgaaaagag tctataatgg
      301 agagaaagtt ttaaaggaga aaatgatgaa aagttcaaaa ctatttgccc ttgcgggcgt
      361 gacattattg gcggcgacta ctttagctgc atgctctgga tcaggttcaa gtactaaagg
      421 tgagaagaca ttctcataca tttatgagac agaccctgat aacctcaact atttgacaac
      481 tgctaaggct gcgacagcaa atattaccag taacgtggtt gatggtttgc tagaaaatga
      541 tcgctacggg aactttgtgc cgtctatggc tgaggattgg tctgtatcca aggatggatt
      601 gacttacact tatactatcc gtaaggatgc aaaatggtat acttctgaag gtgaagaata
      661 cgcggcagtc aaagctcaag actttgtaac aggactaaaa tatgctgctg ataaaaaatc
      721 agatgctctt taccttgttc aagaatcaat caaagggttg gatgcctatg taaaagggga
      781 aatcaaagat ttctcacaag taggaattaa ggctctggat gaacagacag ttcagtacac
      841 tttgaacaaa ccagaaagct tctggaattc taagacaacc atgggtgtgc ttgcgccagt
      901 taatgaagag tttttgaatt caaaaggaga tgattttgcc aaagctacgg atccaagtag
      961 tctcttgtat aacggtcctt atttgttgaa atccattgtg accaaatcct ctgttgaatt
     1021 tgcgaaaaat ccgaactact gggataagga caatgtgcat gttgacaaag ttaaattgtc
     1081 attctgggat ggtcaagata ccagcaaacc tgcagaaaac tttaaagatg gtagccttac
     1141 agcagctcgt ctctatccaa caagtgcaag tttcgcagag cttgagaaga gtatgaagga
     1201 caatattgtc tatactcaac aagactctat tacgtatcta gttggtacaa atattgaccg
     1261 tcagtcctat aaatacacat ctaagaccag cgacgaacaa aaggcatcga ctaaaaaggc
     1321 tctcttaaac aaggatttcc gtcaggctat tgcctttgga tttgaccgta cagcctatgc
     1381 ctctcagttg aatggacaaa ctggagcaag taaaatcttg cgtaatctct ttgtgccacc
     1441 aacatttgtt caagcagatg gtaaaaactt tggcgatatg gtcaaagaga aattggtcac
     1501 ttatggggat gaatggaagg atgttaatct tgcagattct caggatggtc tttacaatcc
     1561 agaaaaagcc aaggctgaat ttgctaaagc taaatcagcc ttacaagcag aaggagtcca
     1621 attcccaatt catttggata tgccagttga ccagacagca actacaaaag ttcagcgcgt
     1681 ccaatctatg aaacaatcct tggaagcaac tttaggagct gataatgtca ttattgatat
     1741 ccaacaacta caaaaagacg aagtaaacaa tattacatat tttgctgaaa atgctgctgg
     1801 cgaagattgg gatttatcag ataatgtcgg ttggggtcca gactttgccg atccatcaac
     1861 ctaccttgat atcatcaaac catctgtagg agaaagtact aaaacatatt tagggtttga
     1921 ctcaggggaa gataatgtag ctgctaaaaa agtaggtcta tatgactacg aaaaattggt
     1981 tactgaggct ggtgatgagg ctacagatgt tgctaaacgc tatgataaat acgctgcagc
     2041 ccaagcttgg ttgacagata gtgctttgat tattccaact acatctcgta cagggcgtcc
     2101 aatcttgtct aagatggtac catttacaat accatttgca ttgtcaggaa ataaaggtac
     2161 aagtgaacca gtcttgtata aatacttgga acttcaagac aaggcagtca ctgtagatga
     2221 ataccaaaaa gctcaggaaa aatggatgaa agaaaaagaa gagtctaata aaaaggctca
     2281 agaagatctc gcaaaacatg tgaaataact gttgcaaaat ataagaaagg atttagtatt
     2341 tctcttgaat gctgaatcct tttttacatt tgtaaagaaa gcttctaaat gtactgaccc
     2401 ccaaaagttg gagcctcttt ttgtcagaat agagaaaatt tttgttaatt ttacttgttt
     2461 cctattgctt tctcagttat tatttgttat attaaaagta taattatttt ttatttatca
     2521 gggttaagca ttgcactttc agaggaagga gtatttttta aaaaggaaat gtaaacgctt
     2581 gctaaaaaat gaaaggattt agaagtttat gaataaagga ttatttgaaa aacgttgtaa
     2641 atatagtatt cggaaatttt cattaggtgt tgcttctgtt atgattggag ctgcattctt
     2701 tgggacaagt ccggttcttg cagatagcgt gcagtctggt tccacggcga acttaccagc
     2761 tgatttagct actgctcttg caacagcaaa agagaatgat ggacgtgatt ttgaagcgcc
     2821 taaggtggga gaagaccaag gttctccaga agttacagat ggacctaaga cagaagaaga
     2881 actattagca cttgaaaaag aaaaaccggc tgaagaaaaa ccaaaagagg ataaacctgc
     2941 agctgctaaa cctgaaacac ctaagacggt aacccctgaa tggaaaacgg tagagaaaaa
     3001 agaacaaaag ggaacagtca ctatccgaga agaaaaaggt gtccgctaca accaactatc
     3061 ctcaactgct caaaatgata acgcaggtaa accagccctg tttgaaaaga agggcttgac
     3121 cgttgatgcc aatggaaatg caactgttga tttaaccttc aaagatgatt ctgaaaaggg
     3181 caaatcacgc tttggtgtct tcttgaaatt taaagatacc aataataatg tttttgtcgg
     3241 ttatgacaag gatggctggt tctgggagta taaatctcca acaactagca cttggtatag
     3301 aggtagtcgt gttgctgctc ctgaaacagg atcaacaaac cgtctctcta tcactctcaa
     3361 gtcagacggt cagctaaatg ccagcaataa cgatgtcaat ctctttgaca cagtgactct
     3421 accagctgcg gtcaatgacc atcttaaaaa tgagaagaag atccttctca aggcgggctc
     3481 ttatgacgat gagcgaacag ttgttagcgt taaaacggat aaccaagagg gggtaaaaac
     3541 agaggatacc cctgctgaaa aagaaacagg tcctgaagtt gatgatagca aggtgactta
     3601 tgacacgatt cagtctaagg tcctcaaagc agtgattgac caagccttcc ctcgtgtcaa
     3661 ggaatacacc ttgaatggac atactttgcc aggacaggtg caacagttca accaagtctt
     3721 tatcaataac caccgaatca cccctgaagt cacttataag aaaatcaatg agacaacagc
     3781 agagtacttg atgaagcttc gcgatgatgc tcacttaatc aatgcggaaa tgacagtacg
     3841 cttgcaagtt gtggacaatc aattgcactt tgatgtgacc aagattgtca accacaatca
     3901 agtcactcca ggtcaaaaga ttgatgacga aagaaaacta ctttcttcta ttagtttcct
     3961 cggcaatgct ttagtctctg tttctagtga tcaaactggt gctaagtttg atggggcaac
     4021 catgtcaaac aatacgcatg tcagcggaga tgatcatatc gatgtaacca atccaatgaa
     4081 ggatttggct aagggttaca tgtatggatt tgtttctaca gataagcttg ctgctggtgt
     4141 ttggagtaac tctcaaaaca gctatggtgg tggttcgaat gactggactc gtttgacagc
     4201 ttataaagaa acagtcggaa atgccaacta tgtaggaatc cacagctctg aatggcaatg
     4261 ggaaaaagct tataagggca ttgttttccc agaatacacg aaggaacttc caagtgctaa
     4321 ggttgttatc actgaagatg ccaatgcaga caagaacgtt gattggcaag atggtgccat
     4381 tgcttatcgt agcattatga acaatcctca aggttgggaa aaagttaagg atatcacagc
     4441 ttaccgtatc gcgatgaact ttggttctca agcacaaaac ccattcctta tgaccttgga
     4501 tggtatcaag aaaatcaatc tccatacaga tggtcttggg caaggtgttc tccttaaagg
     4561 atatggtagc gaaggccatg actctggtca cttgaactat gctgatattg gtaagcgtat
     4621 cggtggtgtc gaagacttca agaccctaat tgagaaggct aagaaatatg gagctcatct
     4681 aggtatccac gttaacgctt cagaaactta tcctgagtct aaatacttca atgaaaaaat
     4741 tctccgtaag aatccagatg gaagctatag ctatggttgg aactggctag atcaaggtat
     4801 caacattgat gctgcctatg acctagctca tggtcgtttg gcacgttggg aagatttgaa
     4861 gaaaaaactt ggtgacggtc tcgactttat ctatgtggac gtttggggta atggtcaatc
     4921 aggtgataac ggtgcctggg ctacccacgt tcttgctaaa gaaattaaca aacaaggctg
     4981 gcgctttgcg atcgagtggg gccatggtgg tgagtacgac tctaccttcc atcactgggc
     5041 agctgacttg acctacggtg gctacaccaa taaaggtatc aacagtgcca tcacccgctt
     5101 tatccgtaac caccaaaaag atgcttgggt aggggactac agaagttatg gtggtgcagc
     5161 caactatcca ctgctaggtg gctacagcat gaaagacttt gaaggctggc aaggaagaag
     5221 tgactacaat ggctatgtga ctaacctctt tgctcatgac gtcatgacta agtacttcca
     5281 acacttcact gtaagtaaat gggaaaatgg tacaccggtg actatgaccg ataacggtag
     5341 cacctataaa tggactccag aaatgcgagt ggagttggta gatgctgaca ataataaagt
     5401 agttgtaact cgtaagtcaa atgatgtcaa tagcccacaa tatcgcgaac gtacagtaac
     5461 gctcaacgga cgtgtcatcc aagatggttc agcttacttg actccttgga actgggatgc
     5521 aaatggtaag aaactttcta ctgagaagga aaagatgtac tacttcaata cgcaggccgg
     5581 tacaacaact tggacccttc caagcgattg ggcaaagagc aaggtttacc tttacaagct
     5641 aactgaccaa ggtaagacag aagagcaaga actaactgta aaagatggta aaattaccct
     5701 agatcttcta gcaaatcaac catacgttct ctatcgttcg aaacaaacca atcctgaaat
     5761 gtcatggagt gaaggcatgc acatctatga ccaaggattt aatagcggta ccttgaaaca
     5821 ttggaccatt tcaggcgatg cttctaaggc agaaattgtc aagtctcaag gggcaaacga
     5881 tatgcttcgt attcaaggaa acaaagaaaa agttagtctc actcagaaat taactggctt
     5941 gaaaccaaat accaagtatg ccgtttatgt cggtgtcgat aaccgtagta atgccaaggc
     6001 gagcatcact gtaaacactg gtgaaaaaga agtgactact tataccaata agtctctcgc
     6061 tctcaactat gttaaggcct acgcccacaa tacacgtcgt gacaatgcta cagttgacga
     6121 tacaagttac ttccaaaaca tgtacgcctt ctttacaact ggatcggacg tctcaaatgt
     6181 tactctgaca ttgagtcgtg aagctggtga tgaagcaact tactttgatg aaattcgtac
     6241 ctttgaaaac aattcaagca tgtacggaga caagcatgat acaggtcaag gaaccttcaa
     6301 gcaagacttt gaaaatgttg ctcagggtat cttcccattt gtagtgggtg gtgtcgaagg
     6361 tgtcgaagac aaccgcactc acttgtctga aaaacacgat ccatatacac aacgtggttg
     6421 gaacggtaag aaagttgatg atgttatcga aggaaattgg tcactcaaga caaatggact
     6481 agtgagccgt cgtaacttgg tttaccaaac tattccgcaa aacttccgtt ttgaagcagg
     6541 taagacctac cgtgtaacct ttgaatacga agcaggttca gacaatacct atgcttttgt
     6601 agtcggtaag ggagaattcc agtcaggtcg tcgtggtact caagcaagca acttggaaat
     6661 gcatgaattg ccaaatactt ggacagattc taagaaagcc aagaaggcaa ccttcctcgt
     6721 gacaggtgca gaaacagggg atacttgggt aggtatctac tcaactggaa atgcaagtaa
     6781 tactcgtggt gattctggtg gaaatgccaa cttccgtggt tataacgact tcatgatgga
     6841 taatcttcaa atcgaagaaa ttaccctaac aggtaatatg ttgacagaaa atgctctgaa
     6901 gaactacttg ccaacggttg ccatgactaa ctacaccaaa gagtctatgg atgctttgaa
     6961 agaggcggtc tttaacctta gtcaggccga tgatgatatc agtgtggaag aagcgcgtgc
     7021 agagattgcc aagattgaaa ccttgaagaa tgctttggtt cagaagaaaa cggctttggt
     7081 agcagatgac tttgcaagtc ttacagctcc tgctcaggct caagaaggtc ttgcaaatgc
     7141 ctttgatgga aacttatcta gtctatggca tacatcttgg aatggtggag atgtaggcaa
     7201 gcctgcaacc atggtcttga aagaaccaac tgaaatcact ggacttcgtt atgttccacg
     7261 tggatcaggt tcaaatggta acttgcgtga tgtgaaactt gttgtgacag atgagtctgg
     7321 caaggagcat acctttactg caactgattg gccagataac aataagccaa aagacattga
     7381 ttttggtaag acaattaagg ctaagaaaat tgtccttaca ggtactaaga cttacggaga
     7441 tggtggcgat aaataccaat ctgcagcgga actcatcttt actcgtccac aggtagcaga
     7501 aacacctctt gacttgtcag gctatgaagc agctttgact aaggctcaga aattaacaga
     7561 caaagacaat caagaggaag tagctagcgt tcaggcaagc atgaaatatg cgacggataa
     7621 ccatctcttg acggaaagaa tggtggaata ctttgcagat tatctcaacc aattaaaaga
     7681 ttctgctacg aaaccagatg ctccaactgt agagaaacct gagtttaaac ttagctcttt
     7741 agcttccgag caaggtaaga cgccagatta taagcaagaa atagctagac cagaaacacc
     7801 tgaacaaatc ttgccagcaa caggtgagag tcaatctgac acagccctct tcctagcagg
     7861 tgttagccta gccctatctg ctctctttgt agtaaaaacg aagaaagact agtatttagt
     7921 aaaacctctt aacaagatta cggaagcagt ctctatcttt tccaatgagg tttatagtac
     7981 agaaaaagcc tgagaagatg tcttctcagg cttttgttaa gcacataaat acaatagtgc
     8041 tatgaaaaaa tcacccagaa aaatctgggt gataaatgtt atggttgtgc tgattgagga
     8101 ttctgatttt gttgatcagg ggttgtattt gattgttgcg tattattgtt aggattggta
     8161 gtcgtactat tatttgtgct tggagtggtt gagctagact gtgaagttga actatctgat
     8221 gatgagcttg aactttcagt tgatgggggt tgttgtggag caggtgagct ccacgtagaa
     8281 cgagcaccat ttttaaatac gaattctcca tttctgtaga gcccctctgg tatattccaa
     8341 tcctctggat tgcttccttc agacaggtag gtcatcatag agcggtaaac tttggcagcg
     8401 accgtaaggc cattgcctac aagtggtgtc agacggttag aatagcctgt ccatacagcc
     8461 attgaatatt tacgcgtata gccagcaaat agttcatcag gtgctacaaa ttgagaggtc
     8521 ttgatgtggt tttcaatttc ctcgtctgta tagttagagg ttcctgtttt accagcctga
     8581 gggagccaag caagataggc atttcgtcca gttccataac tcaagactgt tttcatcatg
     8641 tcggtcatca tataggctgt cgtttccttc atggcacgag ttccgacatt agagaactct
     8701 ttttcactcc catcactaaa gacgacttta tggatataca ttggtttata gtaagttcca
     8761 ccatttgcaa aggcagcgta agcagcagcc atcttttcac tacttgctcc atattttttg
     8821 tctgattcgg ttgtgttact tgaaatggca tttgagtagt gaatacttgg gtagtcgatt
     8881 ccgagaccat ttaggaaagt cttggcgcgg ttgagtccga ccttgtttag agtttccacg
     8941 gctgggacgt ttcgcgattg ttgcagggcg tattgcaagg tgatgttgcc aaagtagccc
     9001 ctatcccagt tataaacagg agtatttgtc ccagggtagt tatagggctc atcgtgaacg
     9061 atagtagcag ttgaatcgta gacaccgtac tccaaggcag gagcatagtc tgtgatcggt
     9121 ttcatagttg atccccagtc gcggtttgtt tctactgctt ggttaattcc gaaggaaaca
     9181 ttacttgact gatggcgtgc tcctagctgg gcaatgactt taccgttaga aacatcaaca
     9241 atggtagaag cgacttgcaa ttcatcgtct ggataggcaa cgtattcgtc tgtattgtaa
     9301 atatcccaca gatgtttttg agcttcttgg tctacatttg tgtagacatc catcccagtt
     9361 gtgagcaggt tatagcctgt ttcttcttca acttgattga tgacttcctt gaggtaatta
     9421 tccatgtaag cagggtaatt acttgctgat ttgagacttt gtagtccatc agtaattggt
     9481 gtattgactg ctttctcata ctgttcagca gagatgtagc cttgattttt catttcagat
     9541 aagaccaagt ttcggcggtc ttgggctgct tctggatgtg aataggggtc atattggttt
     9601 ggtgcctgag gcattccagc cagcaaggct aactgaggta aacttaaatt attgaggtct
     9661 ttaccatagt agttttgagc tgctgtctgc attccatagt tcccattaga catgtagacc
     9721 ttatttatat agtaggtcaa gatttcttgc ttggttgctt tttgttctaa ctgaatcgct
     9781 aaccaagctt cctgagcctt acgagaaata gtctggtcgg aagtcgaagt tgaaaagtaa
     9841 gtcaacttaa tcaactgttg agtgagagtt gatccacctt ggagggaatt gctttgcaga
     9901 ttgcgcaaga aagctcccag gatacggatg gtatcaatcc ccctgtggtc gaagaagcga
     9961 tggtcttcga tagaaacgat tgccttaacc aaatctgtgg gaatatcatt agcttgggca
    10021 ttgacgcggc gttcagaacc caagtcagca atgagttgat ttttattgtc gtagatttta
    10081 ctagaagttg ttgcaactag tttactctcg gataggctag gagccttgct aacgtagtag
    10141 aaaaaaactc ctccgcctaa gacaatggct gcgataacca agcttaagaa gctaatgctc
    10201 agatacttga ttaggcgcag aatcgttggt ttgttcatct tgttttacca cctaataaat
    10261 gttctttgat aacattgaga taaggaattt gagggaaggc accagccttg atttcatatc
    10321 catattctcg aatatattca agtggcattg atttttgtct cttatcttga tgatagaagc
    10381 gaatcaaatc gaatgccggc aataagtagg tttcttgctg agaagaaaag tgaagaagga
    10441 caaagcagat tccttgttgg gcaaggactt gttccatatg ctgaatctga tgtggatgaa
    10501 aatttttcat cggaatcgca cgtttttgtt ttgtttcctt gacttcaaag tcgatgtaat
    10561 atccattata aacgccagaa tagtccgtcg ttgaagcttg tcgaaaatag gcttcaacaa
    10621 tcttggcacg acttcgttgt ggatagtcca cttgtacgat ttgaatagga gttggtttct
    10681 tatgtataac agccaagccc tgagacaaat agtagtcgtt ggtagcattg atcatctttt
    10741 caaaagacat tcctcgattt gcgaaatttt tgggttgaga aagagatgtt tgtctttttt
    10801 gtgatgaaac tttatgtgga tagttgacca taattctcct tattggtaca ataacatcac
    10861 tttattatac cataatttgc acagaaaggg ttaaaaatgg ctacagcttt ggttttaggc
    10921 tattctgctt ttgatttggg tttattttct gataaggatc ctcgtcttaa attgattaaa
    10981 aaagcgatac gtaaagattt agaggctatg gcagcagatg gggtgtcttg gcttgtgttt
    11041 acagggagtt tgggttttga atattgggtt ttagaggttg ctcaggagat gaaaaccgaa
    11101 tacggtttcc agttggccac catttttgct tttgaaaccc atggggaaaa ttggaatgaa
    11161 ggcaatcaga tgaaactgag tcgctttaag caggtagact ttgtcaaata tgcttatcct
    11221 cgctatgaac acaaggggca gttaagagat taccagcagt ttttgctgga aaacacgact
    11281 agttcctatc ttttttatga tgaagaaaat gaaacaaaac tagcttattt ttaccaaaag
    11341 atgaaaaatc aagaggacta tttt
//
LOCUS       DAVBDG010000058        11274 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBDG010000058 DAVBDG010000000
VERSION     DAVBDG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.059"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(400..1536)
                     /gene="dnaJ"
                     /locus_tag="VQ526_001631"
     CDS             complement(400..1536)
                     /gene="dnaJ"
                     /locus_tag="VQ526_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2555333.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1741..2094)
                     /locus_tag="VQ526_001632"
     CDS             complement(1741..2094)
                     /locus_tag="VQ526_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555334.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDYTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(2107..3930)
                     /gene="dnaK"
                     /locus_tag="VQ526_001633"
     CDS             complement(2107..3930)
                     /gene="dnaK"
                     /locus_tag="VQ526_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2555335.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4410..4934)
                     /gene="grpE"
                     /locus_tag="VQ526_001634"
     CDS             complement(4410..4934)
                     /gene="grpE"
                     /locus_tag="VQ526_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2555336.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(4961..5995)
                     /gene="hrcA"
                     /locus_tag="VQ526_001635"
     CDS             complement(4961..5995)
                     /gene="hrcA"
                     /locus_tag="VQ526_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2555337.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRIADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(6159..6668)
                     /locus_tag="VQ526_001636"
     CDS             complement(6159..6668)
                     /locus_tag="VQ526_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2555338.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            7276..9609
                     /locus_tag="VQ526_001637"
     CDS             7276..9609
                     /locus_tag="VQ526_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET2555339.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            9622..11085
                     /locus_tag="VQ526_001638"
     CDS             9622..11085
                     /locus_tag="VQ526_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2555340.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            11085..>11274
                     /locus_tag="VQ526_001639"
     CDS             11085..>11274
                     /locus_tag="VQ526_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HET2555341.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         3438 a         2211 c         2212 g         3413 t
ORIGIN      
        1 aaccataccc atggcaagcc gacgctgacg tggtttgaag aaatttttga agagtatcat
       61 tcaccatttc acaactaaat cgaagacttt ctgccgtaat gaaaacacct gaaacagctt
      121 ggtttcagct gtccggaaac tttaagactt aggttcaaag tttaggtatg gaacttcgaa
      181 ggaggtcgct accgtccgtc attacttagg aaagtcttaa aaaatctata aagtaaaaag
      241 agcccgacag ctctttttgt ttatactcaa tgaaaatcaa aaagcaaact aggaaactag
      301 ccacgggctg ctcaaaacac tgttttgagg ttgtagatac gactcacata tatacggcaa
      361 ggcaacgctg acgtggtttg aagagatttt cgaagagtat tattctccat caaaggcatc
      421 ttttatatgg tcaaagaagc cttttttctt tggatttact ttcaagtcac cagcagtcgc
      481 gaattctttc aaggctactt tttggcggtc gttcaagcct gtcggtgtta cgacattaac
      541 agtaacgtat tggtcaccaa ctgcaccgcc acgaaggctc ggtgccccct tactacgtag
      601 gcggaatttc ttaccagtct gagttccctc tggaataacc aattcaacat caccgtgaac
      661 agttggaata tctactgtat caccaagagc cgcttggaca aagttgaggt tgagattgta
      721 gaagatagtc gttccttcac gttcaaactt gtcgctagct tctacagaaa ctactacata
      781 caagtcacca taaggtccac cgttaaagcc tgcttcacct tgaccagcga ggcgaatttg
      841 ttgacctgtt tccacaccag cagggatttt cacatgtacg ctatgagctt gtttctcatg
      901 acctgttcca tgacaggttg tacatggata tttgatttct tttcctcgac cgtgacagac
      961 atcacaggtt acttggcgac gcatcatacc aagaggagtc tgcgtatcga cgttaatgac
     1021 accagcgcca tgacagcgtc cacaagtgac tggacttgtc cctggcttag caccagagcc
     1081 attacatgta cgacagccag cttcacgatg atacttaact tccttctcag ttccgaagat
     1141 agcttcttca aatgtcaaat tgacacgata ctggagatca tctccttggc gaggagcgtt
     1201 tggattgcgc gaagaaccgc ctccgccgaa gaaacttgag aaaatatcct caaaaccacc
     1261 gaagccacct gccccattga aaccaccgaa accaccagct ccaccaaaac caccattggc
     1321 gcctgcagca ccatactggt cataggcagc acgtttttgg tcgtcactca aagtctcata
     1381 ggcttcttga acttccttgt acttgtcctc agcaccaggc tccttgttga tatctgggtg
     1441 atattttttg gaaagcttac gataagcctt tttgatctcg tctgccgaag cgttttttga
     1501 cacccccaga cgatcataaa attcagtatt gttcatacaa gataccaaga ccgtaaaatt
     1561 cgactgaaaa ataggaaatc tgacgctgga gcgacgctcc tagacagatt tctctttttt
     1621 ccgagaattt aggtcgggtt caattccttt cttttatatt gatgagataa tactccagcc
     1681 actcggccct atcccttttt accgaaaaac agaggctggg ttgcaacctc tgtggttaga
     1741 ttattcattt gaaataaaaa tggttgtatt atctctagta gtaatcttca aagacctttt
     1801 agctaaatca aactcaaatt tattatagtg tgggtcttgt gttttatata ggtgtaactc
     1861 ctgacatata tgatccgaaa agtaaatcat aacatcttta aacacagaag tgtagtcata
     1921 tttactctta ccaacagcta cttctgtaaa ttctttataa atgacatttc catttctttc
     1981 cacagtcacc agcatttctg ttggattata atccaaatta aataattctt caaaaaactt
     2041 tatcttttca tgataatctg caatgtcaga aagattattt ttattaacac tcataacact
     2101 agccccttat ttttccgtaa actctccgtc tacgacgtca tcgcctgcgt ttcctgttgc
     2161 ttgtgcgcct tctgctcctt cttgagcttg ttgcgctgct gcggcttgtt cgtagagttt
     2221 aacagcaagt ccttgagctt tttcgttcaa tgcttcaagt ttcgctttca tgtcgtccaa
     2281 gttgttgtct tcttgagctt tcttaaggtc atcaagggca gcttgggcag cgtcacgttc
     2341 tgcgtcgaag cctttacctt cagtttcctt gattgtcttt tcagtcgcaa agattgcttg
     2401 gtctacttca ttacgaaggt ctacttcttc tttacgtttc ttatcggctt cagcgtttgc
     2461 ttctgcatct ttcatcatgc ggtcgatttc ttcgtcagtc aaacctgagt tcgattggat
     2521 gacaatagtt tgttcttttt gagttccaag gtctttggcc ttaacagaca cgataccgtt
     2581 cttgtcgatg tcaaatgtta cttcgatttg aggaattcca cgaggtgcag ctgggatatc
     2641 agtcaattgg aagcgtccaa gagtcttgtt atctgctgcc attgggcgtt caccttgaag
     2701 aacgtggata tcaacggctg gttggttgtc tgctgctgtt gagaagactt gtgatttaga
     2761 tgttgggatt gtagtgttgc gatcgataag ttttgtaaat actccaccca ttgtttcgat
     2821 accaagtgac aatggcgtta catcaagaag gacaacgtcc ttgacatcac cagtaatcac
     2881 accaccttgg atagccgcac ccatagcaac tacttcatca gggtttactg atttgtttgg
     2941 ttctttacca gtttcagctt taacagcttc aacaacggca gggatacgag ttgaaccacc
     3001 aacaaggata acttcgtcga tttctgacaa gctcaaacct gcatctgaaa gggcttgacg
     3061 aactggaact tttgtacgtt caacaaggtc acgagtcaaa tcatcaaatt tcgcacgagt
     3121 caaagtcatt tccaagtgaa gaggtccagc ctcacctgca gtgataaatg gcaagctgat
     3181 ttgtgttgaa gttacaccag aaaggtcttt cttcgctttt tcagccgcat ctttcaaacg
     3241 ttgcattgcc atcttgtcag tagacaagtc gataccgttt tctttcttga attctgctac
     3301 caagtggtca atgatttttt ggtcaaagtc gtcaccacca agtttgttgt cccctgcagt
     3361 tgacaatacg tcgaagacac cgtcacccaa ttcaaggata gagacgtcga atgtaccacc
     3421 accaaggtca aataccaaga ttttttcttc tttatcagtc ttgtccaaac cataagcaag
     3481 agctgctgca gttggttcgt taacaatacg ttctacttca agaccagcaa ttttaccagc
     3541 gtcttttgtt gcttgacgtt gagcgtcgtt gaagtaagcc ggaactgtga taacagcttt
     3601 ggttactttc tcaccaaggt agtcttcagc gtagcctttc aagtattgaa ggatcatagc
     3661 tgagatttct tgtggagtgt attcttttcc atttgcagaa actttttcag aagttcccat
     3721 cttagatttg atagagataa ctgtatctgg gtttgtgact gcttgacgtt ttgcagcatc
     3781 accaacgatg atttctccgt ttttgaatga gactacagat ggagttgtgc ggtttccttc
     3841 tgggtttgcg atgattttgc tttcagttcc ttcaagaact gcaactgctg agtttgttgt
     3901 acctaagtca ataccgataa ttttagacat gtgtttttct ccttgttagt taattttcta
     3961 tttttctttt tgatactctt cttaagtggc gacgccgtca gagcttgact tcgtcaatct
     4021 ctttgactaa actttgagcc taaggtctca aaatttgcgc aaatagcgcc acggcgaaga
     4081 gtatcgtgtt tggttcgctt cgctcactat tacaaagcca aacatatttt atctttcttc
     4141 atagtttatt gtcgtttcgg acaagttttc tattatgtaa attgcgacac gaagagtcga
     4201 aatcgatttt atttcgacga cgagttagta aggaagctag gcaaacgcca tagcgattgc
     4261 cgttttctga cgagttgctt tagctaccgt cagaattgcc gaatcgaaca cgccctaagc
     4321 tctgagtaaa aaagatagat tttcttgtgt tcatcgaaca cttcgttaat ctcctatttt
     4381 tactgcgagc tttttacggg ctttgtatct tagttataca ccactaccat tgctgggcgt
     4441 aggatgcggt catggagttt gtagcctttt tgaaagactt gagcgatggt atctactggg
     4501 tgttcatcgt ctgctgggag agtttggatg gccatatggt agttatggtc aaattcgcca
     4561 tctgctgcga tttcttcaat tccttcttct ttcaaagcgt gaatcaagct ttcttgcacc
     4621 atccccaagc ccttcttcac atcatctgtc aaaccttcaa ctgcaagtgc acgctcaagg
     4681 ttgtcaagag atggtaagat tgcttttgcc aagtcctggc tacgataacg ttgcaagttt
     4741 tgacgttctt cattggcacg gcgttggata ttttgcattt ctgcatgagc gcgaagatat
     4801 ttgttttcga actcatctgc acgttcattt gccaagtcca actcagactt ttcaggagtt
     4861 gtttcttcag ctgtttccac aacttcctct tcttgaactt cttctacttc ttcatttttt
     4921 atatcttggg ccatgttcgc ctcctttaat gatttcaatc ttaatgtact tcgtaatgat
     4981 tactgctgag gtagcggtaa aaatctgtca acttcatggt caaaacacgg ttgaccacat
     5041 tgatttgatt gattagctgt tggtaatcca gattaactgg accgataatg gctagaattc
     5101 caactccccg ataaggaatg aggaacttac tactaatcac cgctaggtca gctaaacagg
     5161 actcttgacc gtctgcaata cgaacatttt gcatctgatc ctcacgcaac ccctcacgaa
     5221 tctccaaggc caccttttgc ggttggtcaa agaactgata ggctgctaga ttggcaaaat
     5281 tcaagagatt gaccttgccc gccatcacaa tgttttcgtt gaacatttcc ttaaagatgt
     5341 gttcaaagag atcgatgaca ttatccgttg ttgtaaagta acgctggata atctgcggaa
     5401 tctccgtccg aatcttgtag tgaatatcta aaacggtgtg accgaggaaa cgttcctgaa
     5461 tgatgctctt cagtttcagc aaatcctcct gcaagaagtt ccttggaatc agaaactgac
     5521 tagtaaccgt tcgcgactcg tctagggtaa ataccgccaa ggctgtatgt tgccccaaaa
     5581 caacgatatc aaaggctgtc aaacgttgcc tgctcggctc aacatccagt gccactaccg
     5641 tacagccact caggtctgtt agcaagttag cagcctcttg cagaatatcc tccaatttga
     5701 agaattcctg atcaaaggct ttgacaatct catatacctc attttcagcc agccggtgaa
     5761 aatccagtga gtgtttcaca tagtactgaa aaccagcaac acttggcatc cgaccgcttg
     5821 aagtatgagc cttctcaagc aacccttgtt tttctagttc cgccatgtca ttacgaatgg
     5881 ttgcactgct agagttaata gactcttgca aggcttttga tccgacaggt tcgtgcgttt
     5941 tggtaaagat gtcaataatc agatttaaaa tatcctgctg acgctctgta accatctcat
     6001 cactcctctc tgtctgatca attagcactc aatgcatcca agtgctaact tatgattcta
     6061 ttatacaccc ttagaagaga atgtcaagac aaaaactcaa aaaattagca ctcttttatc
     6121 aagagtgcta aaaatcaatc tgaagaccta gaaccttgct attcatctac tcggtatttc
     6181 ctttttagtc tgaaataacc atgaattaga taagaaatca gaaaagcata taaaacaaaa
     6241 ataagaaaga aagacataga aagatcctct ctaaacaaac tcgtacaaac aacaagacca
     6301 cctaagaaag tagctgtaca ggaacgaagt ctagatcgca taacttccca tttgagcccc
     6361 ttactcatat agacttgatc ctctggaagt aaatcagaag acgatttacg aagaatcgaa
     6421 caagaacctg ctcctatcaa ttcccaacct ctatctctaa aatcttgcag ttcatgctta
     6481 tattttttaa gaaatctaga atcatagata cggtagatga catcgtctgg ttgacattgg
     6541 tcaaaataga acaaaccaaa acgactcgtt ctatacctcc aacctttcaa atgcatctca
     6601 tgtaaatatt cttctttctt gtccaaatca acaatggtga aaatccgaaa ttctactctg
     6661 ctattcattg tcttacccca aaattagaaa acatgcctag cgttatttat tagataattc
     6721 tttccacttt tgactcaatc tccaaaaaat ataagaaatc tgaatcgcaa aaactatcaa
     6781 taaaacccaa tctattatga aaatcaaaaa cacttcccaa ctgaaagaac tacctccagt
     6841 gacaaacttt gagaaaaacg gtagtagagc taaaaagaga agcaaaatag gaagcatccg
     6901 cattgttaaa atccgtttgg cataaaaaaa tctttattta aacgaaaata ttatggcaaa
     6961 atttacgcca gtttttgaac ggctgatgta gatattttat actttcaaaa tgtttaaatg
     7021 tgattattta tttttgaaaa atagctcacc agcccgactg aaagtgctta tagaatgata
     7081 ataagtcgcc tgccgaaaac agcggaaaat agcggtgtta tgcggagata atctgacgcg
     7141 atgcgaaagt atattgcata cttattttca acaatttagc agagtatttt tataagtgtg
     7201 atataataga agtataattt gttctgatag tttattttat ggagaagtag atttttagaa
     7261 tgcggagggt tcaatatggt tgagtttata aagtctaaga aagaaatgag tgaggaggat
     7321 attaaagcaa atttcatcac tcctgctatt gtatccaaag gatggaaaaa tggtgagcat
     7381 atcgcttacg aagaatactt cactgatggt cgaattgagg ttagaggaga taaggctcgt
     7441 cgtaaagaag gaaaaaaatc agactattca ctgtattacc aatttggaac tcgaattgca
     7501 attgttgagg caaaggataa taaacacagc gttcgagcag gattacaaca agctattgaa
     7561 tatggagaga ttttagatgt tccatttgtt tattcttcaa atggtgatgg ctttattgaa
     7621 cacgaccgta tcacgagaga agaacgtgag ctggagttag acgaattccc tactcgtgaa
     7681 gaattatttt ctcgtatgac gaaggaaaaa ggattgacgt acgaaattac agaagctatc
     7741 tcaactccat actatacaga cgccttctca atgaaaacgc cacgctatta tcagcaaata
     7801 gctatcaacc gtactattga aacagttgcc agaggacaaa aacgagtaat gtttgtgatg
     7861 gcaacaggaa cggggaaaac gttcatggct tttcaaatta ttcatcgcct tcgaaaagct
     7921 ggtttggcta aacgagtttt attcttagcg gatagaaaca tcttagtaga ccaaacgatg
     7981 gctgaagact ttaagccatt cgaaaaggta atgacgaaaa ttacaccaaa acttttgact
     8041 gctcctgaaa aattaaattc ttttgaaatt tatctagggc tttatcagca actaactggt
     8101 gaagatggaa ctgaaacaca ttatcaaaaa tttgacaaag acttctttga tttaatcgta
     8161 attgatgaag cgcaccgtgg ttcagctaag gaaaacagta actggcgtaa gataattgat
     8221 tatttcagtt ctgcgacaca gattgggatg accgctactc ctaaagaaac caagaatgct
     8281 tccaatacgg aatactttgg tgagccaatc tatacttata gtttaaaaca gggaatcgag
     8341 gatggttttt tggctccata tcgtgttatg agggttaatt tagatgtgga tgtggatggt
     8401 tatcgtccag aaactggaaa agttgatact aacggacaat taatagaaga taggtactac
     8461 ggcaggaaag attttgataa aaccattgtc attgatgata gaacgcaaag agttgccaag
     8521 tttgtttctg attatatgaa gcaaagcaat gcacgatttg ataaaacaat tgttttttgt
     8581 gttgatattg accatgccga gcgaatgcgt gctgcacttg taaaagagaa tctagactta
     8641 gtccaagaag actatcgtta tgtcatgcaa gtaactggtg acaacgctga aggaaaagct
     8701 caactggata actttatgga tgtcaattct aattttcccg ctattgtaac aacgtctaaa
     8761 ttattaacga caggagttaa tgctaaaaca tgtcgtttga ttgttttaga ctctaatatc
     8821 caatccatga ctgaatttaa acaaattatt ggtcgtggca cacgtcttta tcctcaaaag
     8881 gggaaagaat tttttacgat tattgatttt cgaaatgtta ccaatttgtt tgctgaccct
     8941 gattttgatg gtgagccagt gaaggtgctg gaaacaggtg cgaaaacagt cagtggttcc
     9001 acgcccggtt tcgtagatga ggaaggtgac ccagtagaaa aatatatcgt tacagacaag
     9061 caggttacca ttcttaattc tactgttcaa gtattggatg aaaacgggaa actgattacc
     9121 gaaagcctga ccgactacac tcggaagaat atcttaggta gctacgccac tttgaacgat
     9181 tttatcacag tttggcatac ggcggataaa aagaagctta tcttagacga actttataaa
     9241 aaaggagttt atctagatgc tattcgagag tcggagggaa tatcagaaca agaaatcgat
     9301 gattttgatt tactcctaaa acttgcctat ggtcaaaaag aattaaccaa aacggaacgt
     9361 atcaataaac tcaaacaaag cggatattta tataaatata gtgaggaagc gcgtgctgtt
     9421 ttggaaattt tactgaacaa atacatggat aaaggtattg gagaactcga aagcattgaa
     9481 acattaaaac ttccagaatt tcagatatat ggtggaacct tcaaaatcat caatacttat
     9541 tttggagata aaaaacgata tttacaagca attaaagaat tggagcaaga gctatttaca
     9601 gtagcttaat gaaaggaaag tatgtcaatt acatcatttg taaaaagaat tcaagatatc
     9661 actcgaaacg atgctggtgt taatggtgat gctcaacgta ttgagcaaat gtcttggtta
     9721 ttattcttaa aaatttatga tagccgtgaa atggtttggg aattagaaga agacgagtat
     9781 gagtcaatta tcccagagga attaaaatgg cgaaattggg ctcatgctca aaatggggaa
     9841 cgggtattga caggcgatga attacttgat tttgtcaata acaagttatt caaagagttg
     9901 aaagagcttg aaataacttc aaatatgcct attcgaaaaa cgattgttaa atcagctttt
     9961 gaagatgcga acaactatat gaaaaatggc gtcttgttac gccaagtcat caatgttatt
    10021 gatgaagttg atttcaatag ccctgaagat cgtcattcgt ttaatgatat ttacgaaaaa
    10081 attcttaaag atattcaaaa tgctgggaac tcaggagaat tttatacgcc acgtgcagcg
    10141 actgatttta ttgccgaagt tcttgaccca aaacttggag aatcaatggc agaccttgct
    10201 tgcggaacag gaggcttctt gacttcgact ctgaaccgtt taagtagtca acgtaaaact
    10261 agtgaagata ccaaaaaata taatacagct gtttttggta ttgaaaagaa agcatttcct
    10321 catcttttag cagttacaaa tctgtttctt cacgaaattg atgaccctaa aattgttcat
    10381 ggaaatactt tggagaaaaa tgttcgtgaa tatacggatg atgaaaaatt tgacattatt
    10441 atgatgaatc caccttttgg agggtcagaa ttagaaacaa taaaaaataa ctttccagca
    10501 gaattacgga gttctgaaac agctgattta tttatggctg tcattatgta tcgtttgaaa
    10561 gaaaatggtc gtgttggagt tattttacct gatggttttc tatttggtga aggtgtaaaa
    10621 actcgcttga aacaaaaact ggtagatgag ttcaacttgc atacgattat taggttgcct
    10681 catagtgtct ttgcaccgta tacaggaatc catacgaaca ttcttttctt tgataaaaca
    10741 aagaaaacag aagaaacttg gttttatcgt ttagatatgc cagatggtta taaaaatttc
    10801 tcgaaaacta agccgatgaa gtcagaacac ttcaatcctg ttcgtgactg gtgggaaaat
    10861 cgtgaagaga ttctggaagg taagttctac aaatctaaat catttacacc tagtgaattg
    10921 gctgagttga attataattt agaccagtgt ggttttccaa aagaggaaga ggaaatctta
    10981 aatccctttg agttgattca gaattatcaa gcggaaagag caactttaaa tcataagatt
    11041 gataatgtat tagctgatat tttgcagttg ttggaggaca aataatgaca ccagaacaac
    11101 ttaaagcaag tattctccaa agagcgatgg aagggaaatt agtgccgcaa aatcccaatg
    11161 acgaacctgc aagtgaatta ttaaagagaa ttaaagctga aaaagaaaaa cttatcagtg
    11221 aaggaaaaat caaacgagat aaaaaggaaa ctgagatatt tcgtggtgat gatg
//
LOCUS       DAVBDG010000059        11023 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBDG010000059 DAVBDG010000000
VERSION     DAVBDG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.060"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(249..536)
                     /locus_tag="VQ526_001640"
     CDS             complement(249..536)
                     /locus_tag="VQ526_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555342.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(546..956)
                     /locus_tag="VQ526_001641"
     CDS             complement(546..956)
                     /locus_tag="VQ526_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2555343.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            1024..1755
                     /locus_tag="VQ526_001642"
     CDS             1024..1755
                     /locus_tag="VQ526_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555344.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            1752..2801
                     /locus_tag="VQ526_001643"
     CDS             1752..2801
                     /locus_tag="VQ526_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555345.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVEVVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(2969..3298)
                     /locus_tag="VQ526_001644"
     CDS             complement(2969..3298)
                     /locus_tag="VQ526_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2555346.1"
                     /translation="MTDTDPIKRAQTLITDLNKAYQACKQATADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            3575..3913
                     /locus_tag="VQ526_001645"
     CDS             3575..3913
                     /locus_tag="VQ526_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555347.1"
                     /translation="MKHMIIQTQKTVYKVNVDDVYYIQTHPTKAHTVKIVTEEASFNM
                     VQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            3918..4655
                     /locus_tag="VQ526_001646"
     CDS             3918..4655
                     /locus_tag="VQ526_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555348.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITLSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            4669..6009
                     /locus_tag="VQ526_001647"
     CDS             4669..6009
                     /locus_tag="VQ526_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2555349.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGVSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(6053..6208)
                     /gene="blpC"
                     /locus_tag="VQ526_001648"
     CDS             complement(6053..6208)
                     /gene="blpC"
                     /locus_tag="VQ526_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2555350.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FYQPIQL"
     gene            complement(6265..7626)
                     /locus_tag="VQ526_001649"
     CDS             complement(6265..7626)
                     /locus_tag="VQ526_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2555351.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIASLESSIASIKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            complement(7637..9790)
                     /gene="blpA"
                     /locus_tag="VQ526_001650"
     CDS             complement(7637..9790)
                     /gene="blpA"
                     /locus_tag="VQ526_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HET2555352.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINYQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            10072..10326
                     /gene="blpM"
                     /locus_tag="VQ526_001651"
     CDS             10072..10326
                     /gene="blpM"
                     /locus_tag="VQ526_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET2555353.1"
                     /translation="MNTKMMEQFHEMDIAMLSSIEGGKNNWQTNVFEGGSAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            10342..10545
                     /gene="blpN"
                     /locus_tag="VQ526_001652"
     CDS             10342..10545
                     /gene="blpN"
                     /locus_tag="VQ526_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET2555354.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            10789..10938
                     /gene="blpO"
                     /locus_tag="VQ526_001653"
     CDS             10789..10938
                     /gene="blpO"
                     /locus_tag="VQ526_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET2555355.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
BASE COUNT         3146 a         1861 c         2372 g         3644 t
ORIGIN      
        1 aaaacggtgt tttgagcagt ctgtgcctag ctttctagtt tgctttttga tttttattga
       61 gtatgagtta cctaaattat gatgcatagt tgatgggata tatataatag attgaaatag
      121 aatatgaaca aattgataag aggattttaa agtaatctct aacaatgctt tagaaactat
      181 agtgtgctat tctaaattca attcactata acttgtttac gttttaaaaa agagccgggg
      241 ggctcttttt acttatcttc agttccctgc atttctttta tcacagctag tctagtctgg
      301 atatcctttt ccaagacctt aaacttgtaa gtcaagtctt cttggtattc cttgataagt
      361 tctttttgct ggttaatgat ttgcaggctg ttttggataa tatccacatc gtccttgata
      421 gcttgaacgc ggtcagtggt attcaagact tcatctgtga tggtttggcg attttttgta
      481 accagataac ttccggctgc agctcctgca aatagcagta ggttggataa tttcatagca
      541 actccttaag cgtttttgat ggtttcagcg acttgagcaa gtttgtcaaa gtctggttcg
      601 tgggcgataa aatcaatctt gaggtcatcg tcagcactgt agcgaggcac aaggtgaacg
      661 tgagtatgaa aaactgtttg accagcgact tcttcacagt tggaaatgat attcatacca
      721 gcagccttag tgactttcat gactttttga gctacttttg gtacttgggc aaagagttgg
      781 ctggcgctcg tagcatccat ctccaaaaga ttgcgatagt gttcttttgg cacgaccaag
      841 gtgtgtccta gtgttacttg agagatatca agaaaggcaa ggacctgctc atcttcatat
      901 acttttgaag caggaatttc ccctgcgatg attttacaaa aaatgcaatc tgacataaaa
      961 tctacctcta ctgtactgaa ttttgatata atatagctac attataccag atttggagaa
     1021 aatatgttag aaattaaaaa cctgacaggt ggctatgttc atgttcctgt tttgaaagat
     1081 gtgtccttta ctgttgaaag tgggcagttg gtcggtttga ttggtctcaa tggtgctggg
     1141 aaatcaacga cgatcaatga gattatcggt ctgttggcac cttatagtgg ctccatcaat
     1201 atcaatggcc tgactctgca aggagatgcg actagctacc gcaagcagat tggctacatt
     1261 cctgagacgc ctagtctgta tgaggaattg accctcagag agcatatcga aacggttgct
     1321 atggcttacg gtattgagca aaaagtggct ttcgaacgag tagagccctt gttaaaaatg
     1381 ttccgtttgg aacagaaatt agactggttc cctgttcatt tttcaaaagg gatgaagcag
     1441 aaggtcatga ttatctgtgc ttttgtggtg gatccaagtc ttttcatcgt ggatgagcct
     1501 ttccttggtc ttgatccgct ggctatttct gatttgattc agcttttgga agtggagaag
     1561 caaaagggca agtctattct catgagtacc cacgtgctgg attcggcgga gaagatgtgt
     1621 gatgcctttg tcattcttca caagggagag gtgcgtgcca aaggcaatct cctgcaacta
     1681 cgtgaagcct ttgatatgcc tgaggctagt ttgaatgata tttacttggc tctgaccaaa
     1741 gaggaggatc tatgaaagac ttgtttttaa agagaaagca ggcctttcgt aaggagtgtc
     1801 ttggttatct gcgctatgtg ctcaatgacc actttgtctt gttcctgctt gtcctgttgg
     1861 gctttctagc ctaccagtac agtcaactct tacaacattt tcctgaaaat cattggccta
     1921 tccttttgtt tgtaggaatt acgtctgttt tacttttact ttggggagga attgccacct
     1981 atatggaggc tccagacaag ctctttctct tagttggaga agaggaaatc aagctccatc
     2041 tcaagcgtca aactggcatt tccctagtct tttggctctt tgtacagacc cttttcttgc
     2101 tgttatttgc gcccttgttt ttagcaatgg gttatggctt gccagttttt ctgctctatg
     2161 tgcttttatt gggggtagga aaatatttcc actttcgtca aaaggccagc aaatttttca
     2221 ctgaaactgg actggactgg gactatctta tttctcaaga aagcaagcgt aagcaagtct
     2281 tgcttcgttt ctttgccctc tttacgcagg tcaagggaat ttcaaacagt gtcaagcgtc
     2341 gtgcctatct ggactttatc ttaaaggctg ttcagaaggt gcctgggaag atttggcaaa
     2401 atctctatct gcgttcttat ctgcgaaatg gcgacctctt tgctctcagt cttcgtcttc
     2461 tcttactttc cgtactggcg caggttttta tcgagcaagc ttggattgcg acagcagtgg
     2521 tagttctctt taactacctc ttgctcttcc agttgctggc cctctatcat gcctttgact
     2581 accagtattt gacccaactc tttccgctgg acaaggggca aaaggaaaaa ggcttacagg
     2641 aggtagttcg aggattgacc agttttgttt tacttgtgga agtagttgtt gggttgatta
     2701 ccttccaaga aaaactagcc cttctagcct tactaggagc tggtttggtt ttactagtct
     2761 tgtatttgcc ttatcaggta aaacgtcaga tgcaggacta acattgctga tacgacacta
     2821 aaaaagaagt tgagttcagt ctgtctcaac ttcttttttg ttactacagg ataatggttg
     2881 gtccgtagag acttatactc ttcgaaaatc tcttcaaacc acgtcagcgt cgtcttaccg
     2941 tactcaagta cagcttgcgg ctagcttcct agtttgctct ttgattctca ttgagtatta
     3001 acttggtctt gacttggtca aagtggaagc ggtcataggc ccgccaagcg gcgcgagttg
     3061 gagcatctgg atcaagagcg ctgagtccca tgagaagact ggaagtctgg taaaattttt
     3121 ccaattcaat caagaatcga ttatccactg tttcagcctt ggctagaaaa ccaagaatag
     3181 agtttaattg ctcctgaaag cggacgtcgt cagcggttgc ctgtttgcat gcttgatagg
     3241 ctttgtttaa gtcagtaatc aaagtctgag ctcttttgat agggtctgta tctgtcatga
     3301 gaattcctcc tctaatctgg gtgccagtct tgattctagc aactgtgttt tgatactgtc
     3361 agtctatcac ttttatctcc tttttcgcct ttaaatcttt aattgtcatt gaaatgtctt
     3421 gaattgcgct gagtgaattt tatgataaaa tagtggtaag ctcatcatga tattgtagaa
     3481 aaataattct tttaggagct ttcaaagact gtttaggatt gggtgtgctt gtgttagatt
     3541 ttttctgtta ttcttttctt aggaggagaa tccaatgaaa catatgatta ttcagacaca
     3601 gaaaacagtc tataaagtaa acgtcgacga tgtctactat atccaaacac atccaactaa
     3661 agcccatact gtaaagattg ttacagaaga ggctagtttt aatatggttc aaaatttaag
     3721 taatcttgag aaccaatatg gggaaacctt gatgagatgt catcgaaatt gtttggtcaa
     3781 tcttgataaa gtaaaatcga ttgattttca agaaagaatc ctttttcttg gagaagaagg
     3841 tcaatacgct gtcaagtatg ccagacgtcg ctatagagaa attcgtcaaa aatggttgaa
     3901 agaaggagag taagaagatg agaatatttg ttttagaaga tgatttttcc caacagacta
     3961 gaattgaaac gacgattgag aaactcttga aggaacatca tatcactctg agttcttttg
     4021 aagtttttgg taagccagat caattgctgg cagaggtgca tgagaagggg gcccatcagc
     4081 tattcttttt ggatattgag attcgaaatg aggagatgaa gggtctggaa gtggctagaa
     4141 agattcggga tcgggatccg tatgccctga ttgtctttgt gacgactcac tcggagttta
     4201 tgcccctgtc ctttcgctac caagtgtctg ctctggatta tattgataag gccctttcgg
     4261 cagaggagtt tgaatctcgt atcgagacag ccctcctcta tgccaatggt caagacagta
     4321 aaagtctagc ggaagattgc ttttacttta aatcaaaatt tgctcaattc cagtatcctt
     4381 ttaaagaggt ttactatctc gaaacgtcca caagacccca tcgtgttatt ctctatacca
     4441 agacagacag gctggaattt acagcgagtc tagaggaggt tctcaagcag gagcctcgtc
     4501 tcttgcagtg ccatcgctct tttctcatca atcctgccaa tgtggtgcat ttagataaga
     4561 aagaaaaact gcttttcttt cccaatggtg gaagttgtct aatcgcgcgt tataaggtca
     4621 gggaagtgtc tgaggctatt aataacttac actgagctag gagagtttat gaatgttgct
     4681 tggatattgt tgtatacact tgttactcac ggattaaaaa ttgtcatatt ctttaaggta
     4741 gatggaatta gtctcacttt tgagaggatt tttaaggcct ttctttttaa gatactgttg
     4801 gccgttgttt ttggaatgct tggctatatg gtaggaaatg tttacctatc ttattttatg
     4861 gaacccttgt acggcatagg cttatctttc ttattgttaa gagaacttcc taaaaaactc
     4921 cttctctttt atggtctctt tccaatgata ttagtgaatc tcttttatag aggtgtttcc
     4981 tattttgtgc ttccattttt gggacaaggg caagtatatg atgactactc atttatttgg
     5041 ttatgtataa taattttcaa tttcttcatt tctctagctt ttttgaaatg gttggactat
     5101 gatttcacta gcttgagaaa ggggattctc gataaagatt ttcaaaagtc cctgactcag
     5161 attaactgga taatgggggc ttactatttg gtgatacaaa atctgtctta ctttgaatat
     5221 gaacagggga ttcaatcaac gactgttcgc catctcatcc tagtctttta cctgctcttt
     5281 tttatgggga ttatcaagaa attggatacc tatttgaagg acaaactcca tgagagactg
     5341 aaccaagagc aggacttgcg ctacagagag atggagcggt atagtcggca tatagaggag
     5401 ctttataagg aagtacggag ttttcgccat gactacacca acctcttgac tagcttacgt
     5461 ctgggcattg aagaggagga tatggagcag ataaaagaga tctacgactc ggtcttaaag
     5521 gattctagtg aaaaattgca ggacaataaa tatgaccttg gccgattggt gaatgttcgg
     5581 gatcgtgccc tcaaaagtct cctagctgga aaatttataa aagctagaga taagaacatt
     5641 gtctttaatg tcgaagttcc agaggagatt caggtagagg gggtgagtct acttgatttt
     5701 ctaaccgttg tgtctatcct ttgtgacaac gctattgaag ctagtgtaga agcctgtcaa
     5761 cctcatgttt caattgcctt ttttaaaaat ggagcacagg agacctttat cattgaaaat
     5821 tccatcaaag aagagggaat cgatatttct gaaatcttct cctttggagc aagttctaaa
     5881 ggggaggaga gaggagttgg tctctatacc gttatgaaaa ttgtggaaag tcatcccaat
     5941 accagtctca ataccacctg ccaagatcac gtttttcgtc aggtacttac tgtaatacat
     6001 atagaatgaa gaaaaaataa gaccgagagt gcgttctctt ggtcttgttt ttttatagtt
     6061 gtattggctg ataaaatcct tttgtatttt tttgttcaat tatattaaat ctatataaga
     6121 aatcttccca ccatcctcca cctataattt ggttgagttc ggtagttgtt agttcttgaa
     6181 atgaagttag gttttgtttc ttatccatgt tatgattctc ctttttgata agataataaa
     6241 tagttataga gtgttatctg aaaattaatc agaatgggtt aaaattttat ctttgaaata
     6301 atcaaaatat gttttctttg tagttacact agtgacgcga ccttgtaagc catattggat
     6361 gagtttacta tcctcgttag atagttttgc aagagcggtt aatttaaaga gattgccttg
     6421 ctctgttctg gtaggagttt gatcaatact ctgaagttgg ccgatgatgg tggtgccgtg
     6481 atttccaatc ttctccagtt ttaatcttac agtttgtcct ttatctagta aggggagata
     6541 gtcagatgat acgtagtaag tgattagtac ttctcttgta tctgtgatga cagggaatat
     6601 ttgagcaatt tctgtaccag ttggaattct atttttacct tcaaattcgc tgttcagatg
     6661 aacgatacct ttacttggag cggttaaggt attattttct aagcgctgtg tcgcttgatc
     6721 tagttgtact tttaattctg ttaattgatt ctccacggtt agttgttgct gtgaggctgt
     6781 ttgtaaaaac tgagtgcgga gtacttcaat tttggttgct aaactattat cataagttgc
     6841 tacacttccg ataccagctt gctgaatttt gatgcttgcg atagatgatt caagacttgc
     6901 aatactttga ttaatttgag ataaaaatgg ctcctctgcc gttccttgtg tttgtccttg
     6961 agaggctata agataacgat ttaaaattga ctggtgcgga ttgccagttg gtaagcgtgc
     7021 tctgttattt atgatagcat ctctcaactc ttgatattct ccaatttgtt gttgaacttt
     7081 tgtaatttct tgttcgatgg ctgatgaact gctattggta agattagctt gatttgaaac
     7141 ttcagtgtta gtctttgaga tacccagttc aatatcatgg gattgtttag taaaattcat
     7201 aaaggtatta tggtagccaa actcatcctc gctagaaaaa agatcagtcg ctttttctaa
     7261 gctttgtttc aagattccaa gtccttcttt ttgcttctca agtctttgta attgagttgc
     7321 taaggcagtt ttctgacttt cttccattgt ttcagagtat ttgatgagta agtcaccttt
     7381 ctcaactact tgatttgcca ctaaatgatt agcaaggata ggattatcac tggtcgactg
     7441 aatagatgca atgacacttg taggggcgat ttctccttgg gaagtaacag taatttcttt
     7501 tgtggcaaca agggagaaaa tcaagatgaa agtaaatagt aatgaaagag gtataatgaa
     7561 cactgtcgca tagttatggt aacgtctctg ataaaactcg acacttctaa aaagattagg
     7621 attcatgaca ttctccttat ttattgaata gatgatggta gaagccttgc gcctgcatta
     7681 actcttggtg actaccaact tcaatgattt tcccctggtc aagaacaatg acacggttag
     7741 ttcgttcggc tatactgaga cgatgggcta caaagagaat ggttttatca gttagggata
     7801 taagattatc tataaccttt ttctcagtca agacatcaag accgctagta gcttcatcta
     7861 gtattaaaac aggagcttta gttaaaagag cacgagcgag agcgattcgt tgcttttgtc
     7921 ctcctgatag accagctcca tcagagagct gagtttgata gcccataggc attctttcaa
     7981 tgtcttgacg gatttcagct aattcacaag ctcttagaat atcttcttgg ctaatcatat
     8041 gattaccgcc caaggttaaa ttttccaaga tagagccatt aaagatatag gcttgttggg
     8101 gtaggtaatt aatatgacgg cgcaagactt ttttatcaat gtttttaata tcctgataat
     8161 tgatggaaat atgccctttg tagggttcaa agaaattgac aatcattttg gctaaagttg
     8221 ttttaccaga accactaact ccaactaggc taaccttatc tccttgttta atcgtgagat
     8281 taatatctgt taaggtatct cgtccaaaac catacttata agaaaggtca tcaaattcaa
     8341 tatcgcccat caaaaaatgt gaatgaacag ggttttcttg aacttgaaat tcagattcga
     8401 ctagatagac ttcgttcaaa cggttattag cgaccttcgc agattggagt ttggtttgga
     8461 ggttgataat attttccata ggagttgtaa agtaagaaaa aagtgtgtta aaggtaatca
     8521 gctgaccgat agaaatttta ctcgacatga ctaattgagc gccaaaccat aggataagga
     8581 tattcagaac taattttgtt ccctgcttta aactcgtttg taaaatagaa tatttactga
     8641 gcttaaagga tttttccaaa taatctacaa attcgctgtc tatattttga tagcgatttt
     8701 cttcactcgt gagcgacttt atagtttcaa tcccgttgat atcttcgata atggcagagc
     8761 taaccataga attactttgc atgacatcat ggttcatttt ttcgaaaggt ttcataaaag
     8821 aaaagatgat gaacatgtat ataggaatgg aaagaagaga aaggaggaag agattagggt
     8881 tttgtgccag taagacgccc cctacaagaa tcagaataga aacatccaga aaaagagaaa
     8941 gaatggtaga agccaaggca tctataatag agttagcatc tgtgaaccgt gaaatgattt
     9001 ctcctgtacg acgtgtcgca aagaaagaca tgggaagttc aaaaatatgg cgaatatagg
     9061 ataaaatcac atcaatgctt aatctctggc tcagaacggt taggagataa tctctggaaa
     9121 agctcatgac ttgttggagg atataggtga taaccagacc aactgagatg attcctaaag
     9181 ttgatttcat ctgatttggg atgtattcat ccaagattcc ttggagataa taagaaccac
     9241 cgatattgat aatagtgacc aataagcttg agagaacaat gtaagcaatg agagattttt
     9301 gtttgaaaat cagaggaagg aagcttagta gaccattctt tttatcttta tggggttgat
     9361 agctgggttt gggagctaga aaaatagcta ctccagtcca ttcagagaaa aagcgttctt
     9421 ttgacatttt agtgattttt acagaagggt caggatcacc aataatcaga tagtctttct
     9481 ttgtttgata gacaacatag taatgttgga gttttccttc tttgttaacg tgaacgataa
     9541 atggataggg gacatcactc atgtcaaaga gagttttatc tgcttgaaca ggccttgttt
     9601 caaagcccat ttcatcagcg gcttttacaa tgccaagagc agtcgtccct tctttattgg
     9661 tctttgcaag ttctctcaag tgagctagag aaaaatctga accatagaat ttagcaatcg
     9721 aggctaaggc agcaacaccg cagtctctag catctatttg aggaacaaat gtacgtttat
     9781 aagaagtcat tggcaattcc tttcatatag tggatagctc tattatgaca taaaaacaat
     9841 ttcgtgcttt ataaaatctt gaatagtcat tgaaatttcc tgaatggtaa aaaagtgatt
     9901 agaaattatc ttttaaaaaa aatttagagg tagttttaaa taaaaaagct aatttaagac
     9961 gtttcgatga caattcaaga tttggatgaa aaactttaac aaatagtgct atactaaact
    10021 tgtcaaagtt gcaacaagac aaaaattaaa aataaaaaag gagtatttgt tatgaataca
    10081 aaaatgatgg aacaatttca tgaaatggat atagctatgt tatctagtat tgaaggaggc
    10141 aagaataatt ggcaaactaa tgttttcgaa ggtggtagtg ctgcttttgg tggctggggg
    10201 ttagggacag ctatttgtgc tgcgagtggt gttggagcgc catttatggg agcatgtgga
    10261 tacataggag ctaaatttgg tgtggctctt tgggcaggag taacaggggc aacgggtgga
    10321 ttttaacaaa aggagacttg tatgaatact tattgtaata taaatgaaac aatgctgagt
    10381 gaggtttacg gaggtaattc cggaggagca gctgtagttg ctgctttagg ttgtgcagcg
    10441 ggtggagtga aatatgggaa aattctagga ccatggggcg ctgcaatagg aggaattgga
    10501 ggagcagtgg tttgtggata tttagcctat accgctacat cataaaaatg aaagctaaat
    10561 tttatgaagg ctatattctt tattattctc ttcgcttttc aaacctatct tatttatcta
    10621 tccataagta tctctgataa aaaacaaaag actattgaat taacatcatt gaattgtttt
    10681 gtcatattat ttctaatcta tgataaatta atttttctct ttattgccta tgtttttttg
    10741 ataattttta tattaaactt gttccgtaac tgaaaagtga catactatat gaatacaaaa
    10801 atgatgtcac aattttctgt tatggataat gaaatgcttg cttgcgttga aggtggagat
    10861 attgattggg gaagaaaaat tagttgtgca gcaggggttg catatggcgc aattgatggg
    10921 tgtgcaacaa cggtttgaca tttctattgg gaccatttgc tataggaata ggtgtaactg
    10981 gtgctgcagg tggagctata cttggaggtg tagcctatgc agc
//
LOCUS       DAVBDG010000060        11007 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBDG010000060 DAVBDG010000000
VERSION     DAVBDG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.061"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(128..1759)
                     /locus_tag="VQ526_001654"
     CDS             complement(128..1759)
                     /locus_tag="VQ526_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2555356.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2052..6992
                     /locus_tag="VQ526_001655"
     CDS             2052..6992
                     /locus_tag="VQ526_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2555357.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFVGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     AGEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVQAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQKP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHEISTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLDAKPNHPSGS
                     ALAPLLEKNKVLVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7082..8278
                     /locus_tag="VQ526_001656"
     CDS             7082..8278
                     /locus_tag="VQ526_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2555358.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8414..8941
                     /locus_tag="VQ526_001657"
     CDS             8414..8941
                     /locus_tag="VQ526_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2555359.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9018..9374
                     /gene="glnR"
                     /locus_tag="VQ526_001658"
     CDS             9018..9374
                     /gene="glnR"
                     /locus_tag="VQ526_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2555360.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9411..10757
                     /gene="glnA"
                     /locus_tag="VQ526_001659"
     CDS             9411..10757
                     /gene="glnA"
                     /locus_tag="VQ526_001659"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2555361.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10847..10966)
                     /locus_tag="VQ526_001660"
     CDS             complement(10847..10966)
                     /locus_tag="VQ526_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2555362.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT         3512 a         2037 c         2414 g         3044 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatatac tatatatctg cagaatactc tttcagtttc
       61 attcagccta gtcttgctta ggaaaaagta aaaagccatc caattatgat ggatagcttc
      121 ttggttctta gatttgttct gcaaaaacct tttcagcaag gttcatagcg tggtctgata
      181 cacgagtgta gtgtgagatg atgtcgataa agttgacccc agcttgtgtt gaacattcgc
      241 ctttgttgag gcgtttgatg tgggtttttc tgagaacacg ttctatctta ttgattgctt
      301 catgacgttc tacaagactg cgtgcttttt caatatcatt gttttccaca ctatccagag
      361 catctttgat aaagtcacta gtttggcggt aaacttcctc taattctttc aaggcggcat
      421 cagaaaattc aacatttttc cgttgaagat agtcagtcag attgagtaga gcctccgtgt
      481 ggtctccaat ccgttccaaa tcacgggagg aatcaaggat attggtaagc acttcacttt
      541 ctttttggct gagagcttcg cttgaaaggg caatgagata acgtgttaat tgctcatcga
      601 tggtgttaat tgcttcttcg gttttatgcc ctttttcagc aactttttca tccaagtcaa
      661 tgatgtactt ataggaaagg tcaaaggctt tagcagcgta gtttcctaag tgcaagagct
      721 ctttcttagc atttcctaga gcgatagatg gggcctgttt gatgaaatgt tcatcaagat
      781 ataagggttc gtatttgaca acctcgtcct ctccaggaat aatcttggtt acaaagtaag
      841 ccagagctcc gataaatgga aattggacaa tggtgttggt aatattaaag gttccgtgag
      901 caaaggcgat ggtcatttcc ggtgctagat ttagcgtagc ttcaaaccaa tgaatcagga
      961 cagtaaaagg aactagaaaa ataacgcaga caactgttcc gataacgttg aaggcaacat
     1021 gagctcctgc tacccgttta gctgcaatat tagcccctaa agaggcaatg atggctgtaa
     1081 tggttgtccc gatattgtca ccaaatagaa ctggcaaagc tccctgtagg tcaattagat
     1141 tgccggcgta gaggttttgt aaaatcccaa tggtagccga agaagcttga attagcaagg
     1201 tcaagccagt accgacaaag acacccaaaa caggattctt acttagctca atcatatagt
     1261 ccttaaagac ctgtaaatcc ttgagtggag ccattgcgcc gctcatgaga ttgagggcaa
     1321 aaaagatacc accgacacca aagaggatgc gtccgatatt attgactgtc cgattttttg
     1381 taaaaaagag acagacggca ccgataaaga gcataggtag ggcatagtta cctaatttaa
     1441 aaccgatgag aaaggatgtg acagttgtcc caatattagc acccatgaca atcccgatag
     1501 cctgacgtaa ggttaagaga ccggcactga ccaggccgac tgtaataact gttacaccag
     1561 aactagactg aattagagca gtcatcccaa taccaaccag aactccaaag aaaggattac
     1621 tagtatattt gtcaatgtaa aaacgaaggc gatctccagc agcttgttgc aaaccgtctc
     1681 ccatggtctt gatactatat aagaatagcc ccagaccacc taaaaagtga aataaaattt
     1741 cctgccaatt aatggacatt tctttttcct ccgaaaaata atcgcggaat atctcctatt
     1801 ttattttaaa ggataaaagt aaatctaaca agtgttaatg tcaagtttgt aaaagaaaat
     1861 tataattgat aagtgtatta tttttaagag tttttaacct tattttagat ttgtgaaaac
     1921 agctattgtt ttttgtctaa aaatgtttta ggataaaaat gtgattatca tggttctaga
     1981 gtttgataaa aaactacaga atataaagcg ctttcttgaa aacaacaaaa tcaatctttt
     2041 aggaggagaa aatgaagaat ccattttttg aaagacgttg tcgttacagt attcgtaagt
     2101 tatcagtagg agcctgctcg ctgatgattg gtgctgtttt atttgttggt ccagccttgg
     2161 ctgaagaaac tgcagttcct gaaaatagcg gagctaatac agagcttgtt tcaggagaga
     2221 gtgagcattc gaccaatgaa gctgataagc agaatgaagg ggaacatact agagaaaaca
     2281 agctagaaaa ggcagaagga gtagcgacag catctgaaac tgcagaagca gctagcgcag
     2341 ctaaaccaga ggaaaaagca ggtgaggtgg ttgcagaaac accatctgca gaagcaaaac
     2401 ctaagtctga caaggaaaca gaagcaaagc ccgaagcaac taaccaaggg gatgagtcta
     2461 aaccagcagc agaagctaat aagactgaaa aagaagtcca gccagatgtc cctaaaaata
     2521 cagaaaaaac attaaaacca aaggaaatca aatttaattc ttgggaagaa ttgttaaaat
     2581 gggaaccagg tgctcgtgaa gatgatgcta ttaaccgcgg atctgttgtc ctcgcttcac
     2641 gtcggacagg tcatttagtc aatgaaaaag ctagcaagga agcaaaagtt caagccttat
     2701 caaacaccaa ttctaaagca aaagaccatg cttctgttgg tggagaagag ttcaaggcct
     2761 atgcttttga ctattggcaa tatctagatt caatggtctt ctgggaaggt ctcgtaccaa
     2821 ctcctgacgt tattgatgca ggtcaccgta acggggttcc tgtatacggt acactcttct
     2881 tcaactggtc taatagtatt gcagatcaag aaagatttgc tgaagctttg aagcaagacg
     2941 cagatggtag cttcccaatt gcccgtaaat tggtagacat ggccaagtat tatggctatg
     3001 atggctattt catcaaccaa gaaacaactg gagatttggt taaacctctt ggagaaaaga
     3061 tgcgccagtt tatgctctat agcaaggaat atgctgctaa ggtaaaccat ccaatcaagt
     3121 attcttggta tgatgccatg acctataact atggacgtta ccatcaagat ggtttgggag
     3181 aatacaacta ccaattcatg caaccagaag gagataaggt tccggcagat aacttctttg
     3241 ctaactttaa ctgggataag gctaaaaatg attacactat tgcaactgcc aactggattg
     3301 gtcgtaatcc ttatgatgta tttgcaggtt tggaattgca acagggtggt tcctacaaga
     3361 caaaggttaa gtggaatgac attttagacg aaaatgggaa attgcgcctt tctcttggtt
     3421 tatttgcccc agataccatt acaagtttag gaaaaactgg tgaagattat cataaaaatg
     3481 aagatatctt ctttacaggt tatcaaggag accccactgg ccaaaaacca ggtgacaaag
     3541 attggtatgg tattgctaac ctagttgcgg accgtacgcc agcggtaggt aatactttta
     3601 ctacttcttt taatacaggt catggtaaaa aatggttcgt agatggtaag gtttctaagg
     3661 attctgagtg gaattatcgt tcagtatcag gtgttcttcc aacatggcgc tggtggcaga
     3721 cttcaacagg ggaaaaactt cgtgcagaat atgattttac agatgcctat aatggcggaa
     3781 attcccttaa attctctggt gatgtagccg gtaagacaga tcaggatgtg agactttatt
     3841 ctactaagtt agaagtaact gagaagacca aacttcgtgt tgcccacaag ggaggaaaag
     3901 gttctaaagt ttatatggca ttctctacaa ctccagacta caaattcgat gatgcagatg
     3961 catggaaaga gctaaccctt tctgacaact ggacaaatga agaatttgat ctcagctcac
     4021 tagcaggtaa aaccatctat gcagtcaaac tattttttga gcatgaaggt gctgtaaaag
     4081 attatcagtt caacctcgga caattaacta tctcggacaa tcaccaagag ccacaatcgc
     4141 cgacaagctt ttctgtagtg aaacaatctc ttaaaaatgc ccaagaagcg gaagcagttg
     4201 tgcaatttaa aggcaacaag gatgcagatt tctatgaagt ttatgaaaaa gatggagaca
     4261 gctggaaatt actaactggc tcatcttcta caactattta tctaccaaaa gttagccgct
     4321 cagcaagtgc tcagggtaca actcaagaac tgaaggttgt agcagtcggt aaaaatggag
     4381 ttcgttcaga agctgcaacc acaacctttg attggggtat gactgtaaaa gataccagcc
     4441 taccaaaacc actagctgaa aatatcgttc caggtgcaac agttattgat agtactttcc
     4501 ctaagactga aggtggagaa ggtattgaag gtatgttgaa cggtaccatt actagcttgt
     4561 cagataaatg gtcttcagct cagttgagtg gtagtgtgga tattcgtttg accaagccac
     4621 gtaccgttgt tagatgggtc atggatcatg caggggctgg tggtgagtct gttaacgatg
     4681 gcttgatgaa caccaaagac tttgaccttt attataaaga tgcagatggt gagtggaagc
     4741 tagctaagga agtccgtggc aacaaagcac acgtgacaga tatcactctt gataaaccaa
     4801 tcactgctca agactggcgc ttgaatgttg tcacttctga caatggaact ccatggaagg
     4861 ctattcgtat ctataactgg aaaatgtatg aaaagcttga tactgagagt gtcaatattc
     4921 cgatggccaa ggctgcagcc cgttctctag gcaataacaa ggtacaagtt ggctttgcag
     4981 atgtacaggc tggagcaact attaccgttt atgataatcc aaattctcaa actccgctcg
     5041 caaccttgaa gagcgaagtt ggaggagacc tagcaagtgc accattggat ttgacaaatc
     5101 aatctggtct tctttattat cgtacccagt tgccaggcaa ggaaattagt aatgtcctag
     5161 cagtttccgt tccaaaagat gacagaagaa tcaagtcagt cagcctagaa acaggaccta
     5221 agaaaacaag ctacgccgaa ggggaggatt tggaccttag aggtggtgtt cttcgagttc
     5281 agtatgaagg aggaactgag gacgaactca ttcgcctaac tcacgcaggt gtatcagtat
     5341 caggttttga tacgcatcat aagggagaac agaatcttac tctccaatat ttgggacaac
     5401 cagtaaatgc taatttgtca gtgactgtca ctggccaaga cgaagcaagt ccgaaaacta
     5461 ttttgggaat tgaagtaagt cagaaaccga aaaaagatta cctagttggt gatagcttag
     5521 acttgtctga aggacgcttt gcagtggctt atagcaatga caccatggaa gaacattcct
     5581 ttactgatga gggagttgaa atttctggtt acgatgctca aaagactggt cgtcaaacct
     5641 tgacgcttcg ttaccaaggt catgaagtca actttgatgt tttggtatct ccaaaagcag
     5701 cattgaacga tgagtacctc aaacaaaaat tagcagaagt tgaagctgct aagaacaagg
     5761 tggtctataa ctttgcttca ccagaagtaa aagaagcttt cttgaaagca attgaagcgg
     5821 ccgaacaagt gttgaaagac catgaaatta gcacccaaga tcaagtcaat gaccgactta
     5881 ataaattgac agaagctcat aaagctctga atggtcaaga gaaatttaag gaagaaaaga
     5941 cagagcttga tcgtttaaca ggtgaggttc aagaactctt ggatgccaaa ccaaaccatc
     6001 cttcaggttc tgccctagct ccgcttcttg agaaaaacaa ggtcttggtt gaaaaagtag
     6061 atttgagtcc agaagagctt gcaacagcga aacagagtct aaaagatctg gttgctttat
     6121 tgaaagaaga caagccagca gtcttttctg atagtaaaac aggtgttgaa gtacacttct
     6181 caaataaaga gaagactgtc atcaagggtt tgaaagtaga gcgtgttcaa gcaagtgctg
     6241 aagagaagaa atactttgct ggagaagatg ctcatgtctt tgaaatagaa ggtttggatg
     6301 aaaaaggtca agatgttgat ctctcttacg cttctattgt gaaaatccca attgaaaaag
     6361 ataagaaagt taagaaagta tttttcttac ctgaaggcaa agaggcagta gaattggctt
     6421 ttgaacaaac ggatagtcat gttatcttta cagcaccaca ctttactcat tatgcctttg
     6481 tttatgaatc tgctgaaaaa ccacaacctg ctaaaccagc accacaaaac aaagtccttc
     6541 caaaacctac ttatcaaccg gcttctgatc aacaaaaggc tcctaaattg gaagttcaag
     6601 aggaaaaggt tgcctttcat cgtcaagagc atgaaaatgc tgagatgcta gttggggaac
     6661 aacgagtcat catacaggga cgagatggac tgttaagaca tgtctttgaa gttgatgaaa
     6721 acggtcagcg tcgtcttcgt tcaacagaag tcatccaaga agcgattcca gaaattgttg
     6781 aaattggaac aaaagtaaaa acagtaccag cagtagtagc tacacaggaa aaaccagctc
     6841 aaaatacagc agttaaatca gaagaagcaa gcaaacaatt gccaaataca ggaacagctg
     6901 atgctaatga agccctaata gcaggcttag ccagccttgg tcttgctagt ttagccttga
     6961 ccttgagacg gaaaagagaa gataaagatt aaatatcgaa aaatcttgtg aaatctttcc
     7021 ttatatttcc aaagtgtgat ataatagttt cgaataaaat aaataaagga gtttttgtaa
     7081 catggcaaaa cttactgtta aagacgttga cttgaaaggt aaaaaagtcc tcgttcgtgt
     7141 tgacttcaac gtaccattga aagatggcgt aatcactaac gataaccgta tcacagcagc
     7201 tcttccaact attaagtaca tcatcgaaca aggtggacgt gcaattcttt tctctcacct
     7261 tggacgtgtg aaagaagaag ctgataaagc tggtaaatca cttgctcctg tagcagctga
     7321 cttggcagca aaacttggtc aagatgttgt tttcccaggt gtcactcgtg gtgctgaatt
     7381 ggaagcggca atcaacgctc ttgaagatgg acaagttctc ttggttgaaa acactcgtta
     7441 cgaagatgtt gacggcaaga aagaatctaa aaacgatcct gaacttggta aatactgggc
     7501 atcacttgga gatggtatct tcgtaaacga tgcattcggt acagctcacc gtgcacacgc
     7561 atctaacgtt ggtatctcag caaacgttga aaaagcagtt gctggtttcc ttcttgaaaa
     7621 cgaaattgcc tacatccaag aagcagttga aactccagaa cgtccattcg tggctatcct
     7681 tggtggttca aaagtttcag acaagatcgg tgttatcgaa aacttgcttg aaaaagctga
     7741 taaagtcctt atcggtggtg ggatgactta cacattctac aaagcacaag gtatcgaaat
     7801 cggtaactca cttgtagaag aagacaaatt ggatgttgcg aaagctcttc ttgaaaaagc
     7861 aaatggtaaa ttgatcttgc cagttgactc aaaagaagct aacgcatttg ctggttacac
     7921 tgaagtgcgt gacactgaag gtgaagcagt ttctgaaggc ttccttggtc ttgacatcgg
     7981 tccaaaatct atcgccaaat ttgacgaagc tttgactggt gccaaaacag ttgtatggaa
     8041 cggacctatg ggtgtatttg aaaacccaga tttccaagct ggtacaatcg gtgtgatgga
     8101 cgctatcgtg aaacaaccag gagttaaatc aatcatcggt ggtggtgact cagctgccgc
     8161 agcgattaac cttggccgtg cagacaagtt ctcatggatt agtacgggtg gtggagcatc
     8221 aatggaactt cttgaaggta aggttcttcc aggacttgca gccttgacag aaaaataaga
     8281 ttttataaat aaatcaaaga agagagggat gaaagttcct cttttctttt gcttaaaata
     8341 aaaacgcttc ctctcaacta ttactcataa aaatcaccga tttatgataa aatggaaata
     8401 gaaagttgag attatgagtt attttaaaaa atataaattc gataaatccc agttcaaact
     8461 tggtatgcga acctttaaaa caggtattgc tgtttttcta gttctcttga tttttggctt
     8521 ttttggctgg aaaggtcttc aaattggtgc tttgacagcc gtttttagcc tgagggagag
     8581 ttttgatgag agtgttcatt ttgggacttc gcgtattcta ggaaatagta tcggtggact
     8641 ctatgccttg gtcttcttct tattaaatac ctttttccat gaagcctttt gggtgacctt
     8701 ggtagttgtt ccaatctgca ccatgttaac cattatgaca aatgtagcca tgaataacaa
     8761 agcaggggtt attggtggtg tagcagctat gttaatcatt accctatcaa ttccaagtgg
     8821 tgagacaatt tcgtacgtgt ttgtgcgtgt atcagaaacg tttatgggag tttttgtcgc
     8881 aattatcgta aattacgata ttgatcgtat tcgtctcttt ttagagaaaa aagaaaaata
     8941 atgttacatt ttataacatt atcaattgac gtttgtcttt ttttagacta taatagacag
     9001 aaagaaggaa attgtaaatg aaggaaaaag aatttcgccg aaatatggct gtttttccta
     9061 tcggcagtgt tatgaagttg accgatctat cggcgcgtca gattcgttat tatgaagatc
     9121 aagagttgat caagcccgat cgaaacgaag gaaatcgtcg catgtattcc ttgaatgaca
     9181 tggatcgtct gcttgaaatc aaagattata tctctgaagg ttataatatc gctgccatta
     9241 agaaaaaata tgctgaacgt gaagcgaaat ccaagaaagc ggttagtcag actgaagtac
     9301 gtcgtgcact tcacaatgaa ctcctccaac aggggcgttt tgcttcagta cagtcacctt
     9361 ttggtcgcgg ttaggcaacc gcaagtagtc atagattaag gagaaaactt atgccaatca
     9421 cagctgcaga tattcgtcgt gaagtcaagg aaaaaaatgt tacctttatt cgtcttatgt
     9481 tctcagatat tttgggaacc atgaaaaacg tcgaaattcc tgctacagat gaacagttag
     9541 ataaggtctt gtcgaacaag gttatgtttg atggatcttc tattgaaggt tttgtacgta
     9601 tcaatgagtc ggatatgtac ttgtacccgg acttggatac atggacagtc ttcccttggg
     9661 gagatgaaaa tggaagtgtt gcaggtctga tctgtgatgt ctatacaaca gaaggtgaac
     9721 catttgcggg tgaccctcgt ggtaatttga aacgagctct tcgtcacatg gaagaagttg
     9781 gattcaaatc cttcaacctt ggtccagagc cagaattctt cctatttaag ttggatgaaa
     9841 atggggaccc aacacttgaa gtgaatgaca agggtggcta ctttgatttg gcacctactg
     9901 accttgcgga caacacacgt cgtgagattg tgaatgtctt gaccaaaatg ggatttgaag
     9961 tagaagcgag tcaccacgag gttgcggttg gacagcatga gattgacttt aagtacgatg
    10021 aagttctccg tgcttgtgat aagattcaaa tctttaaact tgttgttaaa accattgctc
    10081 gcaaacacgg actttacgca acatttatgg cgaagccaaa atttggtatt gctggctcag
    10141 gtatgcactg taatatgtcc ttgtttgatg cagaaggaaa taacgccttc tttgatccaa
    10201 atgatccaaa aggaatgcag ttgtcagaaa cagcttacca tttcctaggc ggtttgatca
    10261 agcatgctta caactatact gccatcatga acccaacagt taactcatac aaacgtttgg
    10321 ttccaggtta tgaagcgcct gtttacattg cttgggctgg tcgtaaccgt tcgccacttg
    10381 tgcgcgtacc tgcttcacgt ggtatgggaa ctcgtcttga gttgcgttca gtggatccaa
    10441 tggcgaaccc ttacattgct atggctgttc ttttggaagt tggtttgtat ggtattgaaa
    10501 ataaaatcga agcaccagct cctatcgaag aaaatatcta catcatgaca gcagaagagc
    10561 gcaaggaagc tggtattaca gaccttccat caactcttca taatgctttg aaagctttga
    10621 cagaagatga agtggttaaa gctgctctcg gagatcacat ctacactagc ttccttgaag
    10681 ccaaacgaat cgaatgggca agttatgcaa ccttcgtttc acaatgggaa attgataatt
    10741 atttagacct ttactaatat agaagaaaga ttgcctgtgg gtaaacagtc acacagcagt
    10801 ctaagccatc aaatacctat aacccttaaa atagcgattt cggtcgctat ttatttttcg
    10861 aaaaatcctc ttgactcttt tgcgaattag atttatcatc agacgtgtag aagaaaatca
    10921 aggagtcact atggaaaaat tcaatttcaa aaacaatatc gggcaagaaa acaagttact
    10981 tcaaatcgaa atctacaagt ttactaa
//
LOCUS       DAVBDG010000061        10900 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBDG010000061 DAVBDG010000000
VERSION     DAVBDG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.062"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..1073)
                     /locus_tag="VQ526_001661"
     CDS             complement(<1..1073)
                     /locus_tag="VQ526_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET2555363.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGST
                     QAATFSNMANKSQTEQGEINIERDKAKTAVSEYKEKKVSEIYTKLERDRHKDTVDLVN
                     KLQEIKNEYLNKIVQSTSKTEIQGLITTSRSKLDEAVSKYKKAPSSSSSSGSSTKPEA
                     SDTAKPNKPTELEKKVAEAEKKVEEAKKKAKDQKEEDYRNYPTITYKTLELEIAESDV
                     EVKKAELELVKEEAKEPRNEEKVKQAKAKVESEETEATRLEKIKTDRKKAEEEAKRKA
                     AEEDKVKEKPAEQPKPAPAPQPEKPAPKPENPAPKPENPAPKPEKPAEQQAEEDYARR
                     SEEEYNRLTQQQPPKTEKPAQPST"
     gene            complement(1152..1679)
                     /locus_tag="VQ526_001662"
     CDS             complement(1152..1679)
                     /locus_tag="VQ526_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2555364.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            complement(1762..3093)
                     /gene="hk06"
                     /locus_tag="VQ526_001663"
     CDS             complement(1762..3093)
                     /gene="hk06"
                     /locus_tag="VQ526_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2555365.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDIYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            complement(3090..3743)
                     /gene="rr06"
                     /locus_tag="VQ526_001664"
     CDS             complement(3090..3743)
                     /gene="rr06"
                     /locus_tag="VQ526_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2555366.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            complement(4385..6817)
                     /locus_tag="VQ526_001665"
     CDS             complement(4385..6817)
                     /locus_tag="VQ526_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2555367.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYELDRLEEVALELTETDYSQDETFTELPFSRRLQILFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQACSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(6819..7277)
                     /locus_tag="VQ526_001666"
     CDS             complement(6819..7277)
                     /locus_tag="VQ526_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2555368.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            complement(7396..8124)
                     /locus_tag="VQ526_001667"
     CDS             complement(7396..8124)
                     /locus_tag="VQ526_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555369.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(8124..9131)
                     /locus_tag="VQ526_001668"
     CDS             complement(8124..9131)
                     /locus_tag="VQ526_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2555370.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(9169..9927)
                     /locus_tag="VQ526_001669"
     CDS             complement(9169..9927)
                     /locus_tag="VQ526_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555371.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            complement(9890..10180)
                     /locus_tag="VQ526_001670"
     CDS             complement(9890..10180)
                     /locus_tag="VQ526_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2555372.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAVQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(10251..10353)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(10436..>10900)
                     /gene="cbpD"
                     /locus_tag="VQ526_001671"
     CDS             complement(10436..>10900)
                     /gene="cbpD"
                     /locus_tag="VQ526_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2555373.1"
                     /translation="EKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNYQNQS
                     GNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQTGWLK
                     ENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAINTTVDGYRVNSDGERV"
BASE COUNT         3076 a         2506 c         1849 g         3469 t
ORIGIN      
        1 gtagatggtt gtgctggttt ttcagttttt ggcggttgct gttgagtcaa gcgattatat
       61 tcttcttctg atctacgagc atagtcttct tcagcttgtt gttcagctgg tttttctggt
      121 tttggagctg gattctctgg ttttggagct ggattctctg gttttggagc tggtttttct
      181 ggttgaggag ccggcgctgg ttttggttgt tcagctggtt tttctttaac tttatcttct
      241 tctgctgctt ttcgtttagc ttcttcttct gcttttttac gatctgtctt gattttttct
      301 aaccttgtag cctcagtttc ttcactctca actttcgctt ttgcttgctt aactttttcc
      361 tcgtttcgag gttccttagc ttcctctttt actagttcaa gctccgcttt tttaacttcc
      421 acatcggact cagcaatttc aagttcaagc gttttgtaag taatggttgg gtagttacgg
      481 taatcttctt ctttttgatc cttggctttc ttcttagctt cttcaacctt cttctcagct
      541 tctgctacct ttttttctag ttctgtcggc ttgtttggct tcgctgtatc tgaagcttcc
      601 ggtttagtgg aagagcctga acttgacgaa gaagatgggg cctttttata tttagacaca
      661 gcttcatcta gttttgatcg acttgttgta ataagtccct gtatttcggt ttttgaggtt
      721 gattgaacta ttttattcaa atactcgttc ttaatctctt gcaacttgtt aactagatct
      781 acagtatctt tatgtctatc tctctctaat tttgtataga tctcactcac ttttttttct
      841 ttatattcac tgaccgctgt ctttgcctta tctcgttcta tattgatttc tccttgttct
      901 gtctgacttt tatttgccat attagaaaaa gtggctgctt gggtacttcc ctcgttctct
      961 gtcgcatgaa ccacactccc cataacaaga ctggcaacta ctacactagc tactccaata
     1021 ctaaatttac gaattgaata atgtactttt ctttcgcttt ttgatgcaaa catgtttatt
     1081 tccttctata ttttttcttt aaccttacta tatcacttca atatgaactg tatataaatc
     1141 gaattttgta tttatctctt tatgcttcca tttgggatta taacttcatg taatagcttt
     1201 tcctcttcag ctattcgtcg atacaaaata actgcataaa ctggaaaaac taaaacaaaa
     1261 gtcacataag catgactcaa caaggtcaag ccaatcaact caggaagaat atttaaaaag
     1321 taattaggat gttttactgt cctaaacaag atatgatcta catatttgtg attattgaca
     1381 agcataagct tcactgtcca aatatctccc aacaagtgaa tcaccaacat caacatcagc
     1441 atagaaaaaa taagcaagac caaaccaacc atacccatgc catcaaaaat tgtcttgtgc
     1501 accattgcct caaccaagca agagagataa aaaacaatat gagccatagt taatcgtttt
     1561 gtattctcaa ctccaaattc tttcccgcca ttgctaagga ttcgtttctc attctctatc
     1621 gaacgcttta aaaaaatcaa tcgaataaag aatgttacta aaataatcgc taataccata
     1681 ttagtatcct ttcttttatc tatgcaaaga ataacacact tatgttaacc ctatatgaac
     1741 tttaataaaa aactaatctg tctacaagct aatcttaaat tccataccgt gttcataagg
     1801 gagaaaacta tagtccattt gatagctttc taaaatatta ttcacaatgt aaagacccaa
     1861 accggaactt tcatccttat ctgtcacgat ttgagaatga tagaatatat caaataaatg
     1921 ttctagttct tggtcactta gaggcgcaca tgtatttttg atagagagat agccatcttg
     1981 ctcggatatg attacgcgcc catttttatc tgaatattta attgcattac taatcaagtt
     2041 tgtcaaaacc ttatccaatg ccctaagact catgacgacc ctggtagcat ctgtcaattg
     2101 attttcaatt gtaacaccta ttgaatgagc taattcttga taacgtgata aaatatctac
     2161 taaaactggc ttaacagtca aggtctcacg acactctgtc cactcttgga atttagaaga
     2221 ctccagtact tcttctaata agtggctcat ctggtcaatc ttatttatac tctttgcaat
     2281 atattttgga tgatctttgt aatctccaat attatgctgc atattctcta ggataattct
     2341 aagactggct aaaggggttt tcaactcatg tgatgctcca cggacaaagg aaaccttttg
     2401 attttgcaat tttacaattt gctcattacg actttccaac tcatgaataa ccttcaacaa
     2461 gtgctcatac ataccattaa tctgttttcc aacttcacca acttcatctt tcctactttc
     2521 atcaaaacga atattgtcat ccaaatcttg cattttacta gtcacttctg aaatgtaaaa
     2581 aagaggattg agcaagcgtt tagtataaaa ataagaaaaa acaaaggaaa aagcaattgt
     2641 aaccaaaaat gtatatggga gatacaaaag caaaatattc tttgcttctt tgtagatatc
     2701 caccccgtga acaaattgca ctgttacctg tttaccatct gctgtactaa cagacatatc
     2761 taacatcacg atataatttg atagctttcc atctttaata tccaagtcat gcacaagagg
     2821 gagccgctta tctacaatat ctcttttaag gtgagcagat atggtcaagg actttgaata
     2881 agactggagt aagcttggaa tttcatcaga tgtcttagtc tttaaatact ccgtaaacac
     2941 tcttgcgctc tcattaaact tttccccctc cagttgaata taataaaaag gaaaggtcaa
     3001 ataaatagct atatgaatga ctagaccaac accacctaga attgcaaaac ttcttaaaaa
     3061 agacttggtt aataatttag gattttttat cataagctaa tcttataccc aacatttttc
     3121 acagtcacga tacaatctaa agctaatttt ttgcgaagag tacgaatata gacatcaacg
     3181 accctatcac aaggtaaatc agctacatct tttgaaattt cttccaatat ctgctctcta
     3241 cttaaaactt gatttttatg ctgaatcaaa cactttagta ccagtaattc ctttggtttg
     3301 agatcaattt cttcattttt ataatgtgct ttgtaagagg taaaatccac tgttacatcc
     3361 tgatatcgcc aaatatcctc tatgacgtag taacgtctga taagcgcctt aatacgcttg
     3421 acaagcagta ccaaagaaaa aggttttgtc acatgatcat cagcatagag atcaaatacc
     3481 tgtgactgag tagtttcatc tcccagagca ctcaagacga tgacaggaat atcgtgcttt
     3541 tgattgattt cttttaacac ttcaaaacca ctcttcctag gcatcattaa atccagtacc
     3601 atgagatgga tagggagatc ttgaaatttt tccaagacct cttgtccatc cttagccata
     3661 atgacatgat aaccctcttc tgtcagaaat gctgcaattc cttctctaat catttcctcg
     3721 tcatctgcaa ctaaaatgtt catctctctc cctttctact accagaactc catacatcta
     3781 tttcctgtat gctatttgaa aatttcctac cagcctatca tctatgataa tggaatggat
     3841 ttcatttatc cgctactttt tgtcaacaac aacgtcaata caagaaaatc attcatcttg
     3901 cagcttatac tcttcgaaaa tctcttcaaa ccacgtcagc tctatctaca atctaaaaac
     3961 agtgttttga gcaacctgtg gctagctgcc tagtttgttc tttgattttc attgaatatt
     4021 agtttgattt ttcaaatacc agctagaacg gatttgacat gcaaaaaaga aacaggtctc
     4081 ttttgaaaga ataaaaattt cagataagac tgctcttttt ctactacaca cgctctaaga
     4141 caaatacgac ggagggtaat aaacatatga attttgtgtt gaatggtgaa aaccacggat
     4201 cgtttagaag ctataggttt tagtttcata ctcttcgaaa atctcttcaa accacgtcag
     4261 ctctatctgc aacctcaaac cagtgttttg agcaacctgc ggctagctgc ctagtttgct
     4321 ctttgatttt cattgagttt cactccactg tgacttatgg tcagatgctg atacttttat
     4381 tcttttatgc aatatcaaat tttaactggc ctgctttgac accaatctta agtgtgctgc
     4441 ctgccactaa atctccctta agaagaagtt ctgccaactt gtcctccact tctgtttgca
     4501 gggttctgcg aagtgggcga gctcccatct ctgggtcata tccttgattt gctaacaatt
     4561 tcagagctga agcttgtaat ttcaagtcaa tgcctttttc agccaaactt gccactaaag
     4621 gcttgaccat aatcttcacc acttcctgca tatggtcact agataggcta tggaagacca
     4681 ccttctcatc aatacggttg atgaattccg gtctataagc ttttttcagt tcttcaaaca
     4741 tgcgtttttc catattttcc tggtcaaaac gaatatcctt agccccaaaa ccaacagtct
     4801 tatcatcacg aagagcagtc gcacctaggt tcgatgtcat aatgataatg gtatttgaaa
     4861 aatcgacctt gcgtcccttg ctatctgtca aaacaccgtc atccagaact tgcaagagaa
     4921 cattaaagat atctgggtgg gccttctcta cctcatcaaa gaggagaacg gaatagggtt
     4981 tattgcgaac cttctctgtc aactctccac cttcttcgta acccacatag cctggaggag
     5041 ctccatttag acggctggct gcgaatttct ccatatactc actcatatca aagcggataa
     5101 gggctgattc gtcatcaaag agaacttctg ccaaagcctt ggccaactcg gtcttaccga
     5161 cacctgtggg tcctaggaac ataaaggaac cgatcggacg cttgtgactg cgaattcctg
     5221 actgattacg gcgaatcgca cgactaatgc ttgaaacagc ttgatcttga ccgatgacac
     5281 gtttatggag ttctgcttca agatttagat acttcttagc atccgtttga gtcagttttt
     5341 gaactgggat tcctgacaag cgactcaagg tggtcaaaat atcagactct gtcaccaagt
     5401 ctttgtagac aggtacttcc tcttcttttg cgattagctg ggctgcctgt ttccacttgc
     5461 catccatcag ggccttatca gctggactca aatctgaatc gtctgctttt acatacttta
     5521 ccttattttg cactgttgct gctgcttcgt ccaagaggtc gatagcagag tctggcaagt
     5581 gacgactggt taaataacga tgagccatct taaccgctgt ttcaaccgct tcatctgtga
     5641 tttgtacacg gtgatgtttc tcataagtcg ccttcaaacc ttgtaaaata gtcatactat
     5701 ctgccacact tggttcttca atcgtcactt tagcgaaacg acgagaaaga gccgcatctt
     5761 tttcgatatg tttttgatat tcttcctgag tggtggcacc aaccgttctc aaagttccac
     5821 gcgccaaggc tggtttcaag atattggccg catccagagt cgaatcaatc ccgctaccag
     5881 aacccatgat ggtgtggagt tcatcgataa agaggatgac ttggccatct tcttcaatat
     5941 ccttgatgat attattcatg cgttcttcaa agtcaccacg gaagcgtgtc cctgcaacga
     6001 cattcatcaa atcaagttct aacacgcgca tcttagccat ttccgcaggc acgtcaccac
     6061 tagcaatacg ctgagcaaga ccaagcgcca gagctgtttt cccgacacca gcatccccaa
     6121 ccaagacagg gttgttctta gtcttccggc tcaagatttg aatcatacgt gagatttcct
     6181 tgtcccgacc gatgactggt tctaacttgc cagaacacgc ttgctctgtc aaatcatgcg
     6241 tatagtcctc gagaccacca ctaggagtct gcggcatgcc catcatattg gccatagaat
     6301 tttgcttgtc agctactgta cgatggcgtt ggcgtaaagc cttgagatct tcacgagtcc
     6361 agcctgcccg ttcttctaaa tttcgacgaa gagcagcaat cttgacctga tctttcttgt
     6421 cttcataaga aaaaccagcc ctctccaaga tacgagtcgc caaggcattg ccatcatgca
     6481 aaatcgcata gaggacgtgc tctgtcccta gcaccttagc atggaccact gacgctacat
     6541 actctgcttc gtcaaaaaga atctgcaaac gacgggagaa cggcaattcc gtaaaagttt
     6601 catcctggct atagtccgtt tcagtcagtt ccaaagccac ctcttctaaa cggtccagct
     6661 catacggata atcatttaaa gttgcccctg ctacactata actgtgatta gacatggcaa
     6721 tcaacaagtg ccaagactct agataacgag ctccaaaatg tccagcaacc atgtaggcac
     6781 tttcgataca ttcattcaat gcttttgaat agttcatctt acttcccttt tctatctacc
     6841 tcttgtatga tctgacgtag catgtttgct cgaacaactg gagcttcttc tcctaaaacg
     6901 cgatccaaag ctactgattc tagcaaattc atctcctgct tggtcatcaa ttcctgctca
     6961 accaaaagct ggagaatatc ctcataaatt tcttgactga ctcgctcacc aatcgagtaa
     7021 agcagctccc ggagcatttc atgatgacta gaaaactcaa ttcgtcctat acgaatgtag
     7081 cctccaccac cacgcttact ttcaaccaag tagcctctac tttccgtaaa gcgtgtcttg
     7141 atcacgtagt taatctgact aggaacaacc tgaaaggtat ctgccaactg actccgttgc
     7201 aactccacga taccagattg atctaaaatc gccttgatgt aggcctcaat atgatccgat
     7261 gtatttttaa atctcataac aaacccacct ctctctttaa accttgacct tgtttgacta
     7321 ttataccgaa attttctcat ttttaaaaga aaaagggcgc tggtaaagga taatcttcac
     7381 caactcccta tttttctact tatctaagcc taattctgcc aagatttgac gtttgtaggc
     7441 aatcttttgg acttccttgt cctcatcttc agaccaatct agtttaattt ctgaaacaat
     7501 ctgcccaggg cgatttttca agatatagat gcggtcgctg agattgaggg cctcctcaat
     7561 actatgcgtg atgatcaggg ttgttagctg caactgcttg tgaatctcaa gataccaagc
     7621 gtggagttcc atctttgtca tctcatccaa ggcgctaaag gcctcatcta agagaaagag
     7681 cttgtgccca aaaaggtagg tccggagtaa ggctacacgc tggcgcatcc caccgctaag
     7741 ttcatgagga tacttgtctc ttacagctgt cagctggaag gtcgcaagaa ttttatccgc
     7801 tcgggaaata gcttctgcct tatccacctt ttgaatcaag aggggcagaa tgatatttcc
     7861 aagcaccgtc ttgtgctcca agagcagatc cttttgcaac atataactca cgcgcccctt
     7921 gggattttct tcaccatcaa ggacaattct ccctgactga acttctaaaa tcccagcgat
     7981 tagattaaag agggtggtct ttccaacacc acttgggcct aggatggaaa ccacttcgcc
     8041 tgaagtcacc tgtaggttga tatcctctaa aatcctctcc tgaccatagg cataactgac
     8101 gtgctctagt ctaatttctg tcattatttc acaaattcgt tggtgaagcc tttgtctgtc
     8161 aagtcttctt taaggatacc attttcttta tcccatttat agaaagcatt ccagcgagct
     8221 gcgtcaaatt gaccccattt ttccttgtcg cttgcgtatt cttttgacaa gtatttttga
     8281 gattcgatga caaagtcacg tttttccttg agttcaggtg cattcttgat gagaatatct
     8341 gcagcttctt ctggatgttc catggcatat tggtagcctt ttttgatggc ttggatgact
     8401 ttgcgagctt cttctttgtt atctttcaga tagtcgttgt ttgcgatgat aactggtgaa
     8461 tagtagtcaa actccttgac atagtctttc aagtacatga agttagcatc tacaccttga
     8521 gatttagcaa ggataccatc ccaaccgtag taaatccaag cagtatcaaa gacgccattg
     8581 gcaatcggtg tgattgagtt tgagtcgtta tttggtactt tttcaacctt ctcaaagtct
     8641 ccaccttgag attctaccaa ggttttcaac atagcaagtt cagttgggtc attccatgtc
     8701 ccatatttct taccaaccaa gtcttttgga ctgcttacat tatcagattt acgagagatg
     8761 attcctgatg tattgtgttc aacaatagct gcaacggcag tgattcctgc tcctttttcc
     8821 aatttcttag ccatgtagtc ttggaaatac actgcaaatg gtgcctttcc gttgataacc
     8881 aagtcagaag aactttcttc tggtggcaat ttcaaatcaa catccactcc agcttctttg
     8941 aaataacctt tttccttggc aacataaagc cctgtgtggt tggtatttgg tgtccagtct
     9001 aggataaagt caaccttctt aagttctgcc tctttgttgt ctttagaagc agttccttga
     9061 ccacaggcca caagcacaac agctacaaga gctgttacaa gcgttaaaaa cactttccat
     9121 gttttcttca ttttgatttc ctcttttctt tttcaaaaac gctctatttt aagtacgttt
     9181 ccatttaatc acatattttt cactgatatc gaccagcttc atacccaaaa gactgataat
     9241 cgacaccaga ataataatgg caaacatggt atcatactga aacagttttt tagactgaat
     9301 catataaaca ccaagacctt caaaacctcc caaccactca gataccacag ttgtgataaa
     9361 ggcgtaggag acactgaccc tcagacctgc ataaaagtaa ggcaggctaa ccgggatttt
     9421 aaaatgccac aggatttgcc aagggtttgc tcgcatcaga ctaaacaaag tcagcatatc
     9481 cttgtcacaa tgcctaaaac cgtccaaaat actaacgatg atgggaaagg ttgttgttaa
     9541 gataatcaag acaatcttgg gcaaaatccc ataacccagc cacaagacca ggataggagc
     9601 tatggcaatg gtcggaatgg tctgaatgac caccatcata gggtaaatca ggtcattgag
     9661 ccaagtcaaa ctatccatga gcacagccat aagacaggca atcaaaactc ccaaaatcag
     9721 ccccagtaaa gccactctca aggtcgccca gctatggtgc cagagaaatt ctctgtcacg
     9781 aacaaagggc tggagaattt caagaggtgt cggcaggata aacttgggga gaagtttaag
     9841 aaaacctgct aactgccaga ttgacaatac tccgagaaag cccaatagac taatgtgtcg
     9901 tctcagtata cttttcaagt ttctcatcaa tacttaaaat ctctcctaca tttattttga
     9961 cattggcaaa gacattgtct gcctcctgca ctgccacttc cagcgcttct tttagaatgc
    10021 gcataagctc atcaaactcc ccttccaaga ccgtttcaaa tggtgtcact accatcgtca
    10081 cttcttgagt ttgcagataa gcaatgacct gatcaataac agctatccga tcaatcccct
    10141 gtgctagggg taaaacttgc aaggcaatgc ttgctttcat aagaacctcc tcttctcgtt
    10201 ttccttttaa aaaaaagaaa aacctttacc atacatggaa aaggtgcaaa attagactaa
    10261 gtatcgttcc ctccgctggc attacccaga tcaggtgcgg tcgaagttta acacttcctc
    10321 tcagactgta cacagactcc catatattat atgtatatat gatagcgcaa aataagaaaa
    10381 atgtcaagga aactgcttac agaaaagagc aggaaatttc tcctactcca attttctata
    10441 ctcgttctcc atcactgttt actctgtagc catccaccgt cgtattaata gctaaggcgc
    10501 ctgaagagta agaataatac cacttaccag agacctggta ccagcctgtt ttcattgccc
    10561 ctgaactacc cagataatac cacgagccat tctcctttaa ccatcctgtc tgcatttcac
    10621 cagataattt caaataatac cagctagaac cgtctttcag ccatcctgtt gatttagaac
    10681 catttgattt gaaatgatac caactaccat ttattttctt ccaaccgaca gttgaactat
    10741 tattggatcc atagctagag atatttcctg attgattctg ataattttgt gaagaagtca
    10801 ctccctctag ctgtatatag cgacgacttc cgttataagc cgtataactc aaccacttat
    10861 agccgtcttt ttcaagtatc tgatcataat gaaccttctc
//
LOCUS       DAVBDG010000062        10533 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBDG010000062 DAVBDG010000000
VERSION     DAVBDG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.063"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            1..1337
                     /locus_tag="VQ526_001672"
                     /pseudo
     CDS             1..1337
                     /locus_tag="VQ526_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1382..2234)
                     /locus_tag="VQ526_001673"
                     /pseudo
     CDS             complement(1382..2234)
                     /locus_tag="VQ526_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
     gene            2316..2387
                     /locus_tag="VQ526_001674"
     CDS             2316..2387
                     /locus_tag="VQ526_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2555374.1"
                     /translation="MKKISKFFPILMLVMDIIIIVGG"
     gene            2491..3360
                     /locus_tag="VQ526_001675"
     CDS             2491..3360
                     /locus_tag="VQ526_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HET2555375.1"
                     /translation="MKHKKVLIIGYGIMTRGILKILKQSKKYNIYLYSRHLNSTINGI
                     IFIREDELPDAINQVDFIITCFSNHIETDNFINEFLLNIQSLRKTIVIDFTTSRIDCV
                     EKFKKTVEIKGGYYIEAPFTGGKLGSNSGQLSIFLHIDEGVNYNKKELMELLKLISKK
                     IYFFEKSTEPTKFKLIYNSWGAYLLYSIKLFNPLQYDFSPKSLEVANTIVCNDGWMSL
                     VCNSKLEQINDQDFDDIHFRVEYMVKDLEYANFSVFHDEVKEINYLLQQYRDCLVTDS
                     GKDYTVIGKYDNL"
     gene            3347..4069
                     /locus_tag="VQ526_001676"
     CDS             3347..4069
                     /locus_tag="VQ526_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2555376.1"
                     /translation="MITYRNDLLSKELDILISFFKKCEVGKISLVITGSLARGNPRIK
                     DGKLESDIDILVIVDSIQQLISIKKTLEGRFHFVHKISLIFCLKERINRSRYRGIINS
                     IRSVDNLLVDNLHIKNQIIEALDSPTNIVEQTRSMIQEFCYYSSKYLISKNNYLELKL
                     EKYWKEIATLNHIDKKIKHLDFERIFAVLKEHKIQILDSSEYFFQNVKTSENIYLEMR
                     DLVSLENQGLDFEHCILSLGER"
     gene            4066..4596
                     /locus_tag="VQ526_001677"
     CDS             4066..4596
                     /locus_tag="VQ526_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555377.1"
                     /translation="MIINTLGPTATDCFYAINRIKLKVDVIVLYDSFDSIVNCFDKLK
                     GQYILLPVAFESSIKNYSWKDFNFEYHKRIEIVDSFHSFTKGMVLIENCNYKINRAII
                     QPATEILMLNYLKKKSMDLRIDFTHSKVMANKKFLEEGYRFSIVSQETIINNDDIIIC
                     ETFSPEMIWCLYRVKG"
     gene            4600..5694
                     /locus_tag="VQ526_001678"
     CDS             4600..5694
                     /locus_tag="VQ526_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555378.1"
                     /translation="MFSEIIKKYDIVTLKDSGFLRGDWFIGSALMAHDYESLKEGIYF
                     YPQETIWTRILDLEYINPYWEIYNQTYNFLLVNKQDSLAMNFLTSLLREIEVISSKTL
                     DFVVITSNEHLIRNLIDRDYYIITKEIPFFIENSKVGNYFKIFVKNGGQFIPIIDCTE
                     FKIQSKKYFEININKFYLKLLDYFLHGEIPEKFKFFGNTSNFNIESLFLEFINSQENI
                     RVVSLLDCLIQLSSKNIEISGNYKGHTVKKILKKFALYSVITKLDITKVNHLLRNDYI
                     YFLMKEIDNITKKYPKLIQKYPRDILSDRYGITNEVYRYLIGENLHIKINHKLDEVRY
                     FTDIPFIDTSLSIEEFDKIVSKILKIKVGG"
     gene            5697..6368
                     /locus_tag="VQ526_001679"
     CDS             5697..6368
                     /locus_tag="VQ526_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555379.1"
                     /translation="MIRLPNKFKGNNITLEVKLNKAVDASVLYPDVKSLDQELYRQLQ
                     SVESFYLTFSDMYSEINENNEILYTEIIFKIPLCIGEDYFNFPVVSYVSNPYSLIRGF
                     YLGFHKELDCNYVFDDVELHFFREGICEFNFEFKNQNSIITAELPREYNYPLILFNNS
                     NLVNQYGKYTLLDIDKYKLESRKIFEFPEISIGNFLGSEAKSNRVIFSQDKFQINGIK
                     NMEVN"
     gene            6370..7398
                     /gene="galE"
                     /locus_tag="VQ526_001680"
     CDS             6370..7398
                     /gene="galE"
                     /locus_tag="VQ526_001680"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2555380.1"
                     /translation="MKSLLITGGAGYIGSHTVLDLLDNNYEVTIIDDLSNSKKKVIES
                     IKQISKKSRNLHFYKIDLKNEEKLENVFKRHNFDGVIHFAGYKAVGESVVEPLKYYEN
                     NLLSTINILKLMKKYKVFNFVFSSSATVYESTPIMPFYETNPLKASNPYGRTKQYIEV
                     LLNDLFISNSNWKIVCLRYFNPLGAHESGDLGEDPNGIPNNLVPYITQVAIGKLPYLN
                     IFGVDYSTPDGTCIRDYVHVNDLADGHRKALEYIFNTDEGLYEVINLGSGVGFSVFEI
                     LHSLESVIGSYIPYKITSRRAGDMDVSIADISKAEELLGWKPRYDIMKMCQDTWKWQQ
                     KHPNGYDD"
     gene            7422..8666
                     /locus_tag="VQ526_001681"
     CDS             7422..8666
                     /locus_tag="VQ526_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HET2555381.1"
                     /translation="MHIVLFRYSARVVKLKYLYNRNVVIIAPEVQRKKYEDYLAEGNN
                     LPKVIFLEDFILPNIILEIRRISQNTEVESITTLSEEDMDIVGFLNDHFVEKNTHSVS
                     NLLFKDKYFMRSFLVGVVNQPYFRLLESENDLRVFWNNCKSSAAILKPRNEAGSNGIK
                     KVYPNEKIDSVYFEKNYIIEEYIDIDNMITCDGYSIGSEIKRFYVHEYEELLFNTLSS
                     SGYYLVRTSSLYNDSEKIEYIEKALEECRKVLNVFSINSEITPFHFEWFLELSTGKIV
                     FCEVGKRFGGGDIPQLIEDSYGVDVIKEYWDILTNSQVLKKINFSKVISRPANISATF
                     ALYRKQGKVIEVPDEKELAWAKKVYIFIKPNTYYKVSENVVENSMFVQFNCKNSCEFE
                     KRLDELKHISQKFKYVITDSGE"
     gene            8716..9075
                     /locus_tag="VQ526_001682"
     CDS             8716..9075
                     /locus_tag="VQ526_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2555382.1"
                     /translation="MISRIGDIMFDFANNTFLAGLNPTSLSVVAIYQSLESMIGVLFN
                     LFGGVIADSFKRKKIIITTNILCGIACITLSFISQEKWLVYAIVITNVILAFMSAFSG
                     PSYKAFTKEIVKKDSIS"
     gene            9127..9879
                     /locus_tag="VQ526_001683"
     CDS             9127..9879
                     /locus_tag="VQ526_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2555383.1"
                     /translation="MIAIFLYKLLGIHGVLLLDGLSFLIAASLIFFITPVNEEVDTKE
                     NMTIRGIFDDLKIGFTYVYSHKQILTIIALSALVNFFLAAYNLLLPYSNQMFGSISSG
                     LYGTFLTAEAIGGFIGAILSGFINKSLSSKRLMLFLAYSGLMLMLTAPIYYMFRNVII
                     LAFTPALFSLFLSIFNIQFFSIVQRDVDNEFLGRVFGIIFTVAILFMPLGTVFFSVVL
                     NPNNTFNFFIIGVSITILSLIFSTLLKRYDRS"
BASE COUNT         3854 a         1244 c         1795 g         3640 t
ORIGIN      
        1 tcctatcgag attataaaaa atataaggaa tctagtcata aatttgaatt gaaacaacgt
       61 ctttattttt tgcttcagca atctaaatcg tttgatgatt ttttagagaa agcaaagcag
      121 ttgcatgttc agattgattt tagtcaaaag catagccgtt ttttgatgac agatagaacg
      181 atgataaaac caattcgagg tcgccaactt agtaaacgag atttatatga tgaagacttt
      241 tttaaaacat attttgccaa acaagagatt gaaagtcatt tagaattttt gttgaaccgt
      301 gtcaattctt tagaagagtt aataacaaaa gcaaaagaat tgaatctaac cattgactta
      361 aaacaaaaaa atgtaacttt tatcctggaa gaagataatc aaaagataag tttgggtcat
      421 aaaaaataag tgataagaaa ttatatgatg tcaaattttt tcaagattat tttaaaaata
      481 aggaagtcgt tgcttcagaa ggattagaga atttatagga acagtaccat gctttccaag
      541 aagaacgaga taaggataaa gtatccactg aagagattga ggaagccttt gagatattta
      601 aggaaaagag agatgctgtt cgtgaatttg aagtggaact tgcagagaac caaatagaga
      661 agctagttga tgagggaatt tatatcaaag tgtcttttgg tattaagcag agtggtctta
      721 ttttcattcc caattatcaa ttggatacta tggaagaaga caatcagaaa aaatataaag
      781 tctacattcg agaaacgtct tcttactttg tctataataa agaaaacatg gataataatt
      841 gtttcatcaa gggtaggacc ttaatcagac aattgagcaa cgatagccaa aaactcccct
      901 atagacgacc aactttaaaa agtcttcaag agaagatttc taaaattaat cttatgattg
      961 aactatctaa cacgaacaaa caataccaag aaatcaaaga tgagcttgta ttagaaattg
     1021 cagagattga tatgcagcta gaagaaaccc aagaaaaaat cgccacctta aacaagatgg
     1081 cggaagtcct tatcaatttg aagagtgaag atcatgagac tagaaaatta gctaagtatg
     1141 attttgacca aatgaacatg acggaatcaa taatgttaga tcgactcaat acggatattt
     1201 taaaattaca acaggaactt ggtaatgaaa ttaataaata tgaggagata gctagaagat
     1261 tggatttgtt tgtgaaaatt attaatacca ataaatttac agttctaaaa tttcacgaga
     1321 atgccttact agaataatat tatttacgac taattagtta aaagatagag aataatttca
     1381 tttatttttt atttactagt ttattaaaat ggtcagtata gtgtaatttt aacttgtatt
     1441 ctcctaaaat atcgagaact tgtatagctt gtttcattgt ttcaatacca ctacttaatt
     1501 gacgtttaaa ttcatagtaa ccggttgcat agagaaaaac tgtctgttca taaaagttta
     1561 attcattctc taacaatccc tttatctcat taattaaata agagcaatta gaaaattctt
     1621 ctttatctat gctgagaatg aaacaattta tagctagctg agtgacaatc cgcttatttg
     1681 ttttatttag agatgagtaa atataattat ttaacatttc tttagttaag taagaagtag
     1741 gagttgtagt taattgttcg tacacagtta cctaataaaa taatctcata ataaccccat
     1801 ttttcaactt taaagagata atctgctagt tgaagtaatt catcatctga tggaatgatt
     1861 cttgaatcca ttgtgtgtaa aatggatttt accattgttt tttcaaaaga atctaaagtg
     1921 taatttgagg aatccgaaag tagagattgt atattattga tgttttgtaa tgaatattgt
     1981 ttacggatat actgtattaa gttggcaaat gactcagttt ttgtatagtc ttcatcgtgt
     2041 aaaataagaa actcatccaa agtaatgtgt aatttgtcta aaatattgat cagtcggata
     2101 catgatatct ctgactctcc acgttcaaat cgggatattt gcgattttga aagatgctca
     2161 tcagcaacag agcatagact gatttgtttg cctttacgaa cttttctaag tgtgatgcca
     2221 agttttgatt tcatagtttt tcccattttc ccaacaaaaa gttaacttag ttttatttat
     2281 tgtacaataa ataaaaattg aaaggaagtc taaatatgaa gaaaatttca aaatttttcc
     2341 caattttgat gctagtgatg gacattatca ttattgttgg tggttaaatc acttaggtgt
     2401 acacctaagt ctttagagta tctaaagact atacttacta gctaaaacat atttaagtta
     2461 aatcgtaaaa ttttagagag tggtgagact ttgaaacata aaaaagtatt aattataggg
     2521 tatggtataa tgactcgggg tatattaaaa atcctgaaac agtcgaaaaa atataatata
     2581 tacctatata gtaggcatct aaattcaaca ataaatggaa taatatttat tagagaagat
     2641 gaattacctg atgctataaa tcaggttgat tttataatca cttgttttag taatcatatt
     2701 gaaacggata atttcattaa tgagttttta ttaaatatac aatcacttcg taaaaccata
     2761 gtaattgatt ttacaacctc caggattgat tgcgttgaaa aatttaaaaa aactgttgaa
     2821 attaaagggg gatactatat agaggctcct ttcacaggag gtaagttagg aagtaatagt
     2881 ggtcaactat caattttttt acatattgat gaaggtgtaa actataataa aaaggagtta
     2941 atggaattat taaaattaat ttcaaaaaaa atatactttt ttgaaaagag tacggaacca
     3001 acaaaattca agttaattta taactcgtgg ggagcgtatt tgctttactc tattaagcta
     3061 ttcaaccctt tacagtatga tttttcacca aagtctctag aggtagcgaa tacaatcgta
     3121 tgtaatgatg gttggatgag tttagtatgc aactccaaat tagaacagat caatgatcaa
     3181 gattttgatg acatacactt tagagtagaa tatatggtaa aagatttaga atatgctaat
     3241 ttttcagtct ttcatgatga agtgaaagaa ataaattatt tattacagca atatagggat
     3301 tgcttagtaa ctgatagtgg aaaagattat acggtaatag gaaagtatga taacttatag
     3361 gaatgacttg ctgtctaaag aattagatat actaatttca ttttttaaaa aatgcgaggt
     3421 aggaaaaatt tcactcgtta ttactggttc cttggcaaga ggtaatccaa gaatcaagga
     3481 tgggaaatta gaatcggata ttgatattct agttatagta gacagtattc agcaattaat
     3541 tagtataaaa aagactttag agggaaggtt tcactttgta cataaaattt ctttgatttt
     3601 ttgtttgaag gaaagaataa atcgaagtcg ttatcgtgga attattaata gtattagaag
     3661 tgtagataat cttttagtag ataatctgca tataaaaaat caaataatag aagctttaga
     3721 ctcacctacc aatattgtgg agcagactcg ctctatgatt caggaatttt gttattattc
     3781 atcaaaatat ttaatttcaa agaataatta tttggaatta aaactagaaa agtattggaa
     3841 ggagattgca acattaaatc atatcgataa aaagataaaa cacctagatt ttgaaagaat
     3901 atttgctgtt ttaaaagaac acaaaataca aattttggat tcaagtgaat attttttcca
     3961 aaatgtaaaa acttctgaga atatttattt agagatgagg gatttagtat ctttggaaaa
     4021 tcaaggatta gactttgaac attgtatttt gagcttgggg gaaagatgat aataaataca
     4081 ttaggaccaa ctgcaacaga ttgtttttat gcaataaatc gtataaaatt aaaagtagat
     4141 gtgattgtac tgtatgatag ttttgatagt atagttaatt gctttgataa attaaaaggg
     4201 caatatatat tattacctgt agctttcgag agtagtatta aaaattatag ttggaaagat
     4261 ttcaactttg aatatcataa gcggattgaa attgtggatt cttttcactc ttttactaag
     4321 ggaatggttc taatcgagaa ttgtaattat aaaattaata gggcgattat ccaacctgca
     4381 actgagattt tgatgttaaa ctatttaaag aaaaaaagta tggatctgag gatagatttt
     4441 acccatagta aagttatggc aaataaaaaa tttctggaag aaggttatcg tttttcaata
     4501 gtaagccaag aaacgataat taataatgat gatattatta tttgtgaaac tttctctcca
     4561 gaaatgattt ggtgcttata tagagtgaaa ggatagaaaa tgttttctga aattattaag
     4621 aaatatgata ttgttacact gaaagatagt ggttttttga gaggtgattg gttcataggt
     4681 tcagcgttaa tggctcatga ctatgaaagc cttaaggaag ggatatattt ctatccccag
     4741 gaaacaattt ggacaagaat cttggattta gaatatataa atccgtattg ggaaatatat
     4801 aatcaaactt ataatttctt attagttaat aaacaagatt cattagcaat gaatttttta
     4861 actagtcttc taagagaaat agaagttatt tcgtctaaaa cattagattt tgttgttatt
     4921 acttcaaacg aacacctaat taggaattta attgatagag actattatat tattacaaaa
     4981 gagattccat tttttattga aaactcaaaa gttggaaatt attttaaaat ttttgtgaaa
     5041 aatggaggac agtttattcc tataattgac tgtacggaat ttaagattca gtctaagaaa
     5101 tattttgaga taaatattaa taaattctac ctgaaacttt tggattactt tcttcatgga
     5161 gagattccag aaaaatttaa attttttgga aatacctcta atttcaatat agaaagcttg
     5221 tttctagaat ttattaatag ccaagaaaat attcgagtag tttccttgtt ggattgtctt
     5281 attcaattat cctctaaaaa tattgaaatt tcaggaaatt ataaagggca cacagtgaaa
     5341 aaaatactaa aaaaattcgc tttatactca gtaattacca aacttgatat aacgaaagta
     5401 aatcatttat tgagaaatga ttatatctac tttttaatga aggaaattga caatattact
     5461 aaaaaatatc caaagttaat tcaaaagtac cctagagata tcttgagtga taggtatgga
     5521 ataacaaatg aagtatatag atacttaatc ggagagaatt tacatataaa aattaatcat
     5581 aaattagatg aggttagata ttttacagat attccattta ttgatacttc actttctatt
     5641 gaagagtttg acaaaatagt ttctaaaata ttaaaaatta aagtgggagg gtaactatga
     5701 taagattacc aaataaattt aaaggtaata atataactct agaagttaaa ctaaataaag
     5761 cagttgatgc tagtgtatta tacccagatg ttaagtctct agatcaagaa ttgtatcgac
     5821 aactacaatc agtagaatca ttctatctta ccttttctga tatgtattca gaaattaatg
     5881 aaaataatga aatcttatat acagaaataa tctttaagat acctctttgt attggagaag
     5941 attattttaa ttttccggtt gtatcatatg tttcaaatcc ctattcgtta ataagaggat
     6001 tttatctagg ttttcataag gaattggact gtaactatgt atttgatgat gtagagcttc
     6061 atttttttag ggaagggatt tgtgaattta acttcgaatt taaaaatcaa aattcaatca
     6121 ttacagcaga attaccaagg gagtataact atcccttgat cttgtttaat aattccaatc
     6181 tggttaatca atatggaaaa tatactttgt tagacataga caaatataaa ttagagtcta
     6241 gaaagatttt tgaatttcca gaaatttcta tcggtaactt tcttggaagt gaagcaaaat
     6301 caaatagggt aatttttagc caagataaat ttcaaattaa tggcataaag aatatggagg
     6361 taaattagca tgaaatcatt attaataaca ggtggagcag gatacatagg atcacataca
     6421 gttttggact tgttagataa taattatgaa gtaacaatca ttgatgattt gagtaactct
     6481 aagaaaaaag ttattgagtc tattaagcag atatccaaga agagtagaaa tcttcatttt
     6541 tataaaattg atttaaaaaa tgaagaaaaa ttggaaaatg tttttaagag acataatttt
     6601 gatggagtaa ttcattttgc agggtataaa gctgttggag aatccgtggt tgaaccattg
     6661 aagtattatg aaaataattt attatcaact ataaatattt taaaactaat gaaaaaatat
     6721 aaggtattta attttgtgtt tagttcatca gctactgtat acgaaagtac tcctatcatg
     6781 ccattctatg agactaatcc attaaaagct tcaaacccgt atgggagaac aaaacagtat
     6841 atagaagtac ttttaaatga tttgtttatt tcgaattcaa attggaaaat tgtttgtctg
     6901 aggtatttta atccattggg tgcacatgaa agtggtgatt taggagagga tccaaatgga
     6961 attccgaata atcttgttcc ttacattaca caagttgcaa ttggaaaatt accatattta
     7021 aatatattcg gagttgatta cagtactcct gatgggactt gtataaggga ctatgttcac
     7081 gttaacgatt tagcagacgg acacagaaaa gcattagagt atatttttaa tacagatgaa
     7141 ggtttatatg aagtaatcaa tttaggaagt ggtgttggtt ttagtgtatt tgaaatccta
     7201 cacagcttag agagtgtcat aggctcgtat attccatata agataacttc aaggagagca
     7261 ggagatatgg acgtttcaat tgcagatatt tcaaaagcag aagagctact gggatggaag
     7321 ccaaggtatg atattatgaa aatgtgccaa gatacgtgga aatggcaaca gaaacatcca
     7381 aatggatatg atgactaata attgaaagga ttgggattaa catgcatata gtattattta
     7441 gatattctgc acgggttgtt aaactaaaat atctgtataa cagaaatgta gtaattattg
     7501 cacctgaggt tcaaagaaaa aaatatgaag attatctagc tgaaggaaat aatctaccta
     7561 aggtcatttt tttagaagat tttattctac caaatatcat cttagaaatt agaaggataa
     7621 gtcaaaatac agaagttgag agtattacaa ccttgagtga ggaagacatg gatatagtag
     7681 gttttttgaa tgatcatttt gtagaaaaaa atactcactc tgtgtctaat ttattattta
     7741 aagacaaata cttcatgcgt tcatttttag ttggagtagt aaatcaaccg tattttaggc
     7801 ttttagaatc agaaaatgat ttacgtgttt tttggaataa ttgtaaatct tctgctgcaa
     7861 ttttaaaacc tagaaatgaa gcaggaagta atggaataaa aaaagtttac cctaacgaga
     7921 aaattgattc agtgtatttt gaaaaaaact atattataga ggaatacatt gatatcgata
     7981 acatgataac atgtgatggt tattcaattg gaagtgagat taagcgattt tatgttcacg
     8041 aatatgagga actattgttc aataccttaa gttctagtgg atattatctt gtaaggacta
     8101 gttcgttata taatgatagt gaaaaaattg aatacataga aaaagcattg gaagaatgtc
     8161 gcaaagtttt gaatgttttt tctatcaatt ctgaaataac cccttttcat tttgaatggt
     8221 tccttgaatt atcaactggt aaaatagtct tttgtgaagt tggaaaaaga tttggagggg
     8281 gagatattcc ccagttaatt gaggatagtt atggagttga tgtaattaaa gaatattggg
     8341 atattttgac taattcacaa gtattgaaaa aaataaattt ttctaaagtg atttctagac
     8401 ctgctaatat ctcagcaacg tttgctttat atagaaagca aggtaaggtt atcgaggtac
     8461 ctgatgaaaa agaattggct tgggcaaaaa aagtgtacat atttattaaa ccaaatactt
     8521 actataaagt atctgaaaat gtagtcgaaa attcaatgtt tgtccaattt aattgcaaaa
     8581 atagttgtga atttgagaaa aggttagatg aacttaagca catatcacaa aagtttaaat
     8641 atgtgattac tgattcggga gaataaaatg attagagaag aacatcacaa tattctatcc
     8701 attgtagcta gtaggatgat atctcgaatc ggagatatca tgtttgattt cgccaataat
     8761 acctttcttg ctggtcttaa tccaacatct ctatcagtgg ttgcaattta tcaatcatta
     8821 gaaagtatga taggtgttct atttaattta tttggtggag ttattgcaga tagtttcaag
     8881 cggaaaaaaa ttattattac tacaaatatc ttatgtggta ttgcttgcat aactctttca
     8941 tttatatccc aagaaaaatg gttggtttat gcgatagtca taacaaatgt cattttggct
     9001 tttatgagtg ctttttcagg gccatcatac aaagctttta cgaaagaaat tgtaaaaaag
     9061 gacagtatat cataacttaa ttcattgcta gaaacaacaa gtactgtgat taaagtaaca
     9121 attcctatga ttgcaatttt cttatataag ttacttggaa tacatggtgt gttattacta
     9181 gatggattat cgtttttaat tgctgcctca ctaattttct tcattactcc tgtaaatgag
     9241 gaggtggata caaaagagaa tatgacgata agaggaatct ttgatgattt aaaaatagga
     9301 tttacgtatg tatatagtca taaacaaatt ctcactataa ttgccctttc agcacttgtt
     9361 aactttttcc tagcagccta taatttgttg ctgccataca gtaatcaaat gtttggaagt
     9421 atttcaagtg ggctatatgg aaccttttta acagccgaag cgattggtgg ctttattggt
     9481 gcgatattaa gtggatttat caataaatcc ttgtcaagca aacgtttaat gctcttttta
     9541 gcatattcag gtttgatgtt aatgctaaca gcgccgatct attatatgtt ccgcaacgtc
     9601 attattttag cctttactcc agcattattt agtctatttc tttccatttt taatatccaa
     9661 tttttctcta ttgttcaaag agacgtagat aatgagtttc ttggcagagt atttggaatc
     9721 atctttacgg tagctattct ttttatgcca ctaggtactg tttttttctc agtagtttta
     9781 aatcctaaca atacttttaa tttttttatt attggtgtat ctattacgat attatcgcta
     9841 atatttagca cgctattgaa gaggtatgat agaagttgat agatgataaa aaatagctga
     9901 tatagagaat ttagagcgcc tttggaatat atttttggaa tttaatcaat cacagttgaa
     9961 tgtgaagggc aatccttttt agaagaatac aatcaagata ccattatgct atttctttag
    10021 ttcagttaaa atattgagaa gtattacttt tattgaaaaa gcatcacaaa ggactccaat
    10081 taatttttaa tgatttttct aatctaattt tgtggttatt gacaaataaa gtctagttaa
    10141 ttgctttgaa atccatggag tggtctatat acatctgtta atgcaacgga aaattttatt
    10201 gataagtagg gggtggctat tattcagctt ttaggttctg aaagtaagag attcacatta
    10261 gaaatatcat gttgaccatt tttcaatttg ctggttagta gtaatctgga catcattaat
    10321 cccttcctta acaaaattat gtgacttttg ttaccggatt actgctgatt ttttaatagc
    10381 ttataacatc tttagacggt taatagattt tgaccgtctt tcttaatatt gtcttaccta
    10441 taatagatat gcttatgaga tatgtttaat aaattaattt tagttcaaaa tgtccagttt
    10501 ggacactttg aatgatttct tagttttata tat
//
LOCUS       DAVBDG010000063        10328 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBDG010000063 DAVBDG010000000
VERSION     DAVBDG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.064"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            94..1269
                     /gene="mutY"
                     /locus_tag="VQ526_001684"
     CDS             94..1269
                     /gene="mutY"
                     /locus_tag="VQ526_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2555384.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNYGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1325..2029
                     /locus_tag="VQ526_001685"
     CDS             1325..2029
                     /locus_tag="VQ526_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555385.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2022..3371
                     /gene="vicK"
                     /locus_tag="VQ526_001686"
     CDS             2022..3371
                     /gene="vicK"
                     /locus_tag="VQ526_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2555386.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3373..4182
                     /locus_tag="VQ526_001687"
     CDS             3373..4182
                     /locus_tag="VQ526_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2555387.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKNKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4273..4515
                     /locus_tag="VQ526_001688"
     CDS             4273..4515
                     /locus_tag="VQ526_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2555388.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVANLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4517..4771
                     /locus_tag="VQ526_001689"
     CDS             4517..4771
                     /locus_tag="VQ526_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2555389.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4818..5561
                     /locus_tag="VQ526_001690"
     CDS             4818..5561
                     /locus_tag="VQ526_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2555390.1"
                     /translation="MKKKCFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLIGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDVVPTSIFGKWLAVLLMLVGIGTIGMLTSTLTNFFVKDNPDE
                     QIKLDKLKDELSSQRILIEKQSEKIEELH"
     gene            complement(5851..6837)
                     /locus_tag="VQ526_001691"
     CDS             complement(5851..6837)
                     /locus_tag="VQ526_001691"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2555391.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7031..9499
                     /gene="gyrA"
                     /locus_tag="VQ526_001692"
     CDS             7031..9499
                     /gene="gyrA"
                     /locus_tag="VQ526_001692"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2555392.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            9499..10242
                     /gene="srtA"
                     /locus_tag="VQ526_001693"
     CDS             9499..10242
                     /gene="srtA"
                     /locus_tag="VQ526_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2555393.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3212 a         1752 c         2292 g         3072 t
ORIGIN      
        1 gtataagtag atagaaaagt tatatactat ctattgaaaa tattataaat cagatacaaa
       61 gacaggtgaa accttctgtg ttatactaga gatatgttag atttgaaaga atacggtatc
      121 gtcatgtggc cggaggagaa agtcatttct ttccgtgaga aacttctcgc ttggtatgat
      181 gaaaacaaaa gagatttgcc ttggaggaga agtaaaaatc cttatcacat ctgggtatct
      241 gaaatcatgc ttcagcagac cagggtggat acagttatcc cttactacga aagatttttg
      301 gactggtttc caactgtcga aagtctggca actgcgcctg aggagagttt actgaaagct
      361 tgggagggct tgggctatta ttctcgagtt cgcaatatgc aggctgcagc ccagcagatt
      421 atgactgact ttggtggcca atttccaaat acctatgaag gaatttccag cttgaaaggg
      481 attggacctt acacagcagg agccatttcc agtattgctt ttaacttgcc tgagccagct
      541 gtagatggta atgtcatgcg ggtcttggcg cgtctgtttg aagtcaacca cgatattggg
      601 attccaagta atcgaaaaat ttttcaggca atgatggaga tcttgattaa tccagatcgt
      661 ccgggtgatt ttaatcaagc cttgatggac ttagggtctg atattgagtc tcctgtaaat
      721 cccagacctg aagaaagccc agtcaaggac tttagtgcgg catatcagaa tggaaccatg
      781 gaccgttatc caattaaatc tcctaagaaa aagccggttc ctatttatct taaagcctta
      841 gttgtgaaaa attctcaagg acaattttta cttgaaaaaa atgaaagcga aaagctcttg
      901 gcaggttttt ggcatttccc ctttatagaa gttgataact tttcgcaaga agagcagttt
      961 gacctctttc atcaggttgc ggaagaaagt gtgaactatg gccccagtcc agaagagagt
     1021 ttccagcagg actatgacct agatgttgat tggcttgatg tttgttttga tacagtccag
     1081 catgttttta gtcatcgaaa atggcatgta cagattgtag caggtcaggt gagtgacttc
     1141 catgattttt cagataggga agttcgttgg ctttcaccag aagaattcaa gaattaccca
     1201 cttgccaaac cccaacaaaa aatctggcag gcttatgcac aagccaactt agacagtagc
     1261 caagactagc tattgtgtct tctgactatt tttttggtat aatagtagag aaaaaggtga
     1321 acatatgaaa aaaatactaa ttgtagatga tgagaaacca atctcggata ttatcaagtt
     1381 taatatgacc aaggaaggtt acgaagttgt aactgctttt aatggtcgtg aagcgctaga
     1441 gcaatttgaa gcagagcaac cagatattat tattctggat ttgatgcttc cagaaattga
     1501 tggtttagaa gttgctaaga ccattcgtaa gacaagcagt gtgcccattc ttatgctttc
     1561 agccaaagat agtgaatttg ataaggttat cggtttggaa cttggggcag atgactatgt
     1621 aacgaaaccc ttctccaatc gtgagttgca ggcgcgtgtt aaagctcttc tgcgtcgttc
     1681 tcaacctatg ccagtagatg gtcaggaagc agatagtaaa cctcaaccta tccaaattgg
     1741 ggatttagaa attgttccag acgcctacgt ggctaaaaaa tatggcgaag aactagactt
     1801 aacccatcgt gaatttgagc ttttgtatca tttagcatcg catacaggtc aagtcatcac
     1861 gcgcgaacac ttgcttgaga ctgtctgggg ttatgactat tttggtgatg tccgtacagt
     1921 tgatgtgact gtacgacgtc tgcgtgagaa gattgaagat acgcccagcc gaccagagta
     1981 tatcttgaca cgccgtggtg tagggtatta catgagaaat aatgcttgat ttactgaaac
     2041 aaaccatttt taccagagat tttatcttta tcctgatttt gttaggtttc atccttgttg
     2101 tgaccctctt attactggaa aatagacgtg ataatattca gttgaagcaa atcaatcaaa
     2161 aggttaaaga tttgattgca ggagattatt ccaaggttct tgatatgcaa ggtgggtctg
     2221 aaatcaccaa tattaccaat aatttgaatg acttgtcgga ggttattcgt ctcactcagg
     2281 aaaatctaga acaagagagt aagaggctaa atagtattct gttttatatg acagatgggg
     2341 ttcttgcgac caaccgtcgg ggtcagatta tcatgattaa cgatacagcc aagaagcaac
     2401 tggggttggt taaggaagat gttctgaata gaagcatttt ggaattgctc aagatagaag
     2461 aaaactatga attgcgtgat ttgattaccc aaagtccaga attgttgcta gattcccaag
     2521 atatcaatgg tgaatatttg aaccttcgag ttcgctttgc cttgatacgt cgagagtctg
     2581 gctttatttc aggtttggtg gctgttttgc atgatacgac ggagcaggag aaggaagaac
     2641 gcgaacgaag actctttgtt tccaatgtta gccatgagtt acggactcct ctgactagcg
     2701 taaaatccta tcttgaagcc ttggatgagg gggctttgtg tgaaactgta gcaccagact
     2761 ttatcaaggt ttctcttgat gagaccaacc gtatgatgcg catggtgacg gatctcctcc
     2821 atctttcacg tattgataat gctaccagtc acctagatgt ggaactgatt aacttcactg
     2881 cttttattac ctttatcctc aatcgttttg acaagatgaa aggacaggaa aaggagaaaa
     2941 aatatgagtt ggtgagagat tatcccatca attctatctg gatggaaatt gatacagata
     3001 agatgacgca ggttgtcgac aatattttaa ataatgctat taaatattcg ccagatgggg
     3061 gtaaaatcac tgtcagaatg aagacaactg aagaccagat gattttatcc atttctgacc
     3121 acggtttggg gattcctaag caggatttac cacgtatctt tgaccgtttc tatcgtgtgg
     3181 atcgtgctag aagtcgtgca caaggtggta caggtctagg actgtctatc gctaaagaaa
     3241 ttatcaaaca acataagggc tttatttggg ccaagagtga atacggcaag ggttcaacct
     3301 ttaccattgt gctcccttat gataaggatg cagtgaaaga agaagtatgg gaggatgaag
     3361 tagaagacta gaatgagtga aataggcttt aaatacagta ttttagcgtc gggttccagt
     3421 ggaaattctt tttatctgga aacctcaaaa aataagcttt tagtagatgc aggcttgtct
     3481 ggcaagaaaa ttaccagtct gctagctgaa attaaccgta agccagaaga cctggatgcc
     3541 atcttgatta cccatgagca ttcagatcat atccatggag taggcgtttt ggctcgcaag
     3601 tatggtatgg atctttatgc caatgaaaag acctggcaag ctatggaaaa tagtaaatat
     3661 cttggcaagg tggattcttc gcaaaagcat atttttgaaa tgggtaaaac caaaaccttt
     3721 ggagatatcg acatcgagag ttttggtgta agccatgatg cagtcgcacc gcagttctat
     3781 cgctttatga aggatgataa gagttttgtc cttttgacag atacaggtta tgtcagtgac
     3841 cgtatggcgg gcattgtcga aaatgcggat ggctatctta tcgaggccaa ccatgatgta
     3901 gagattttgc gatcaggttc ttacgcttgg cgactcaaac aacgaatcct atctgacctt
     3961 ggtcaccttt ctaacgagga cggtgctgaa gctatgattc ggacgctagg aaatcgtact
     4021 aagaagattt accttgggca tttatctaag gaaaataata tcaaggaact ggctcatatg
     4081 accatggtca atcagctggc tcaagctgat ctgggagtcg gagtagactt taaggtttat
     4141 gatacctcac cagataccgc aacaccattg acagagatat aaaaagaacg ctgagaaggt
     4201 gttcttttta tattgactga acacctaaaa agtaatacaa tggtgttacc attaaaaaag
     4261 ggagcacaaa agatgactac tattacatta aaagtttctg aagctgataa aacatttatg
     4321 aaagcaatgg ctaagtttga aggagtttcc ctttcggaac ttattcgcac caaaactctt
     4381 gaagccctag aagatgaata cgatgctcgt gtggcaaatt tagcctatca agagtattta
     4441 gaagacttgg aaaaaggagt tgaacccatt acttgggaag aaatgatgca tgatttaggc
     4501 ttgaaggatg aataatttgt ataaattagt tccaacaaga cgttttatca agcaattgaa
     4561 aaaattggac cgttatacgc agaagctaat tacaaactat ttacaaacca atgttttgga
     4621 aggcccaaga cgacacggaa aggctttggt tggtaatcgc gttggtcaat ggcgctatag
     4681 aattggtaat tatcgagtta tcgtacaaat tgtagatgat gaattagtcg ttgctactct
     4741 agaagttggt catcggagag atatttattg aattaaagct gagtaataac aatatagaaa
     4801 gtaaggagag taaaaatttg aagaaaaaat gcttttttgc agattattat gatacaacaa
     4861 ttattttact tgctttaatc tctgttattc tggtattact tggatttgct gagatgattg
     4921 atttggacaa tccgccttat agtattattg atttagtaat ctggggtgtt tttgtaattg
     4981 attatagttg gcgctttttt attactaaaa gaaaatggcg ctttattctt gaaaatattt
     5041 ttgatttatt ggctattctc cctttaaatg ctattttcac agtttttcga ttagggcgta
     5101 tctttcgttt agttaaacta acaaaattac tgaaactgac tcgtttactt agaataattg
     5161 gacttatagg taaattagaa agaaaaatta gtagattctt acggacaaat ggcctgattt
     5221 atatcttata tgttaatatc tttattgttc tagtagggag ttcgatttta tccgttgttg
     5281 aagaaaaatc cttctcagat agtctctggt gggctcttgt gacggtaact actgttggtt
     5341 atggtgatgt tgttcctact tcaatttttg gtaaatggtt agctgtctta ttaatgcttg
     5401 tcggtattgg gacaatagga atgttaacga gtaccttgac gaactttttt gtaaaggata
     5461 atccagatga acagataaaa cttgataaac tcaaagacga attatcgagc caaagaatat
     5521 taatagagaa acaatctgaa aaaatcgaag aactacattg aatgataaaa gacttagttg
     5581 aaaaaatatc ataaaggtga gacaaaacaa aacttgattc gaggagaaat caagtaagtc
     5641 ttgacacaat agaacgcata ttatcaggta taaaaacttg atactatgcg ttttatagag
     5701 aaataaaata aaatctgaac aaatcaatta ggaatttaaa gcattttata acaacgtttt
     5761 agagtgatag ggtgctattt caacttcaac ctactataat acagaaaaaa aacaactccc
     5821 tgatgattca aggagttgtc tatagttaaa ttagttttta gaagcttctt ggaattctgg
     5881 atttttccat gcttcgtcaa taatagcttg caattcttta gcagatgctt gcattttttg
     5941 agtttctgca tcgttcaatg gaatgtttac tggacgaacg ataccatgtg caccaacaac
     6001 agctggttga ccgataaaga cattctcaac tccgtattga ccttcttgga atactgaaag
     6061 tggaagtact gcgttttcat cgtcaaggat tgctttagtg atacgagcaa gggctactgc
     6121 gataccgtag tatgttgcac cttttttgtt gatgattgtg taggctgcat cacgaacacc
     6181 ttcgaacaat tcaatcaatt cagcttcttg aacattttga gtgtctttaa ggaattcttc
     6241 aaggtttaca ccagcgatgt tagcgtgtga ccaaacagcg aactcagagt caccgtgttc
     6301 acccatgatg taggcgtgca ctgaacgagc atccacatcc aatttttcag caagtgcttg
     6361 acggaaacga gctgagtcaa gtgaagtacc tgaaccgata acgcgttctt tagggaaacc
     6421 agagaatttc caagttgagt aagtcaaaac gtcaactggg ttagcagcaa caaggaagat
     6481 acctttgaaa ccagattcaa caacttgagt tacgattgat ttgttgatag caaggttttt
     6541 acctacaagg tcaagacgag tttcacctgg tttttgaggt gcacctgcag tgatcacaac
     6601 aaggtcagcg tctgcacagt cagagtattg agctgcatag atttttttag gtgaagtgaa
     6661 ggcaagggcg tgactaaggt caagcgcatc accaacagct ttttcatgca attgtggaat
     6721 ttcgataatt ccaagctctt gtgcaattcc ttggttaaca agtgcaaaag cgtaagatga
     6781 acctacagca ccatcaccga caaggataac ttttttgtgt tgtttagttg aagtcattgt
     6841 tttaaacatc tccttaattt tattagggga ttttccctag acaacttcat tctatcactt
     6901 ttaaaaaact ttgtcacgaa tatgccttat agttctcgat gtaaacgttt tagtggttta
     6961 gaggctgaaa tagatgggaa tttatggtat aatgttgtta cttactaatt gtgaaatgag
     7021 gcatttatta atgcaggata aaaatttagt gaatgtcaat ctgacaaagg agatgaaggc
     7081 aagttttatc gactacgcca tgagtgttat cgtagcgcga gctcttcctg atgttcgaga
     7141 tggcttaaaa cctgttcacc gtcgcattct ctacggaatg aatgaattgg gtgtgacccc
     7201 agacaaaccc cataaaaaat ctgctcgtat tacaggggat gtcatgggta aatatcaccc
     7261 acacggggat tcctctattt atgaagccat ggtccgtatg gctcaatggt ggagctaccg
     7321 ttacatgctt gtagatggtc acgggaattt tggttccatg gatggagata gtgctgccgc
     7381 tcaacgttat accgaggcac gtatgagcaa gattgctctg gaaatgcttc gtgatatcaa
     7441 caaaaataca gttgatttcg ttgataacta tgatgccaat gaacgggaac ccttggtctt
     7501 gccagcgcgt tttccaaacc ttttggttaa tggagcaact ggtatcgcgg ttgggatggc
     7561 aaccaatatt ccacctcata atctgggtga aaccattgat gcagtgaagt tggtcatgga
     7621 taatcctgaa gtgactacca aggacttgat ggaagtcttg cctggaccag attttccaac
     7681 tggtgctctt gtcatgggga aatcaggtat ccataaggct tatgaaacag gtaaaggttc
     7741 gattgcccta cgttctcgta cagagattga aacgactaag actggtcgtg agcgcatcgt
     7801 tgtaacagaa tttccttaca tggtcaataa aaccaaggtg catgagcata ttgttcgctt
     7861 ggttcaggaa aaacgcattg agggtatcac agcagtacgt gatgagtcaa accgtgaagg
     7921 tgttcgattt gttattgaag tcaagcgcga cgcctcagcc aatgttattc tcaataacct
     7981 cttcaaaatg acccaaatgc aaaccaattt tggtttcaat atgctcgcta tccaaaatgg
     8041 tataccgaaa attttgtctc ttcgtcagat tttggatgct tatatcgagc accaaaaaga
     8101 agtggttgtt cgtcgtacac gttttgataa ggaaaaagcg gaagcgcgcg ctcatatctt
     8161 agaaggtctc ttgattgcgc tagaccatat cgacgaagtg attcgtatca tccgtgctag
     8221 tgaaacggat gcggaagctc aagctgagtt gatgagcaag tttaagcttt ctgaacgtca
     8281 aagtcaagct atccttgata tgcgtcttcg tcgtttgaca ggtttggaac gcgataagat
     8341 tcaatctgag tatgatgacc tcttggctct gattgcggat ttagcagata ttcttgctaa
     8401 gcctgaacgt gtttctcaaa ttatcaaaga cgaattggat gaagttaaac gtaaattttc
     8461 tgataagcgc cgtacagagt tgatgattgg acaggtcttg agtctcgagg atgaggactt
     8521 gattgaagaa tcggatgtct tgattaccct ttctaacaga ggctacatta agcgtttgga
     8581 tcaggacgag ttcactgctc aaaaacgtgg gggtcgtggt gtccaaggaa cgggagtgaa
     8641 agatgatgac tttgttcgtg agttagtgtc aactagcacc catgatcatc tgctcttctt
     8701 cacaaacaag ggacgtgtct atcgtcttaa aggttatgaa attcctgagt atggtcggac
     8761 tgccaaaggg ctaccagtag tcaatctctt gaaattggat gaagacgaaa gtattcagac
     8821 ggttatcaat gttgagtctg atcgcagtga tgatgcttat ctcttcttta caacccgtca
     8881 cggtattgtg aagagaacca gtgttaagga gtttgccaat attcgtcaaa atggtctcaa
     8941 agcgctgaat ttaaaggatg aagatgagtt aatcaatgtc ttgttggcag aaggagatat
     9001 ggatattatc attggtacca agtttggtta tgcagttcgc tttaatcaat cagccgttcg
     9061 tggtatgagt cgtatcgcca ctggtgtaaa aggtgttaac cttcgtgaag gagacacagt
     9121 tgttggtgcc agcttgatta ctgatcaaga tgaggttctt attatcacag aaaaaggata
     9181 tggtaagcgt acagtcgcta ctgaataccc aacaaaaggt cgtggtggta agggaatgca
     9241 gacagctaaa attaccgaaa aaaatggctt gctggccggt cttatgactg ttcaagggga
     9301 tgaggatttg atgattatca ctgatacagg tgtcatgatt cgaaccaatc ttgccaatat
     9361 ttcacaaaca ggacgtgcaa ctatgggagt taaagtaatg cgcctggatc aagatgctca
     9421 gatagtgact ttcacaacgg ttgcggtggc agaaaaagaa gaagttggga cagaaaacga
     9481 aacagaaggt gaagaataat gtctcaaaaa aataataaaa agaaaaacaa gcgaaaaaat
     9541 ctgctgacaa atatcctagc aggatttctg atattactgt cactggcttt gatttttaat
     9601 actcaaattc gaaatatttt catagtctgg aataccaata agtatcaagt tagccaggta
     9661 tcaaaagaaa aattagaaga aaatcaggat acagaaggca attttgactt tgattctgtc
     9721 aaagctatct cttcggaagc tgttctaact tctcaatggg atgctcaaaa attaccagtt
     9781 attgggggaa ttgcaattcc tgaattggaa atgaatttgc cgatttttaa aggacttgat
     9841 aatgttaatc tcttctacgg agctggtaca atgaaacgcg agcaagtaat gggagaagga
     9901 aattatagtc tagctagtca ccatatcttt ggtgttgata atgctaataa aatgttattt
     9961 tctcctttag ataatgctaa aaatggcatg aagatttatc taaccgataa aaataaagtt
    10021 tatacttatg aaatacgtga agtcaaacgt gtgacaccgg atcgtgttga tgaagttgat
    10081 gatagagatg gggtcaatga aatcacatta gtaacctgtg aagaccttgc tgctacagaa
    10141 cgtattattg tcaaaggtga tttgaaagaa acaaaagatt attcacaaac atctgatgaa
    10201 attctaacag ctttcaatca accatataaa caattttatt aatacaaatc agtgaaatca
    10261 tggatttcac tgatttttta agattttcat aaaaatatag taaaatgaaa taagaatagg
    10321 acgaatcg
//
LOCUS       DAVBDG010000064        10184 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBDG010000064 DAVBDG010000000
VERSION     DAVBDG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.065"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            435..1613
                     /locus_tag="VQ526_001694"
     CDS             435..1613
                     /locus_tag="VQ526_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2555394.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLFQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRFFVSAN
                     LLETFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            1927..2058
                     /locus_tag="VQ526_001695"
     CDS             1927..2058
                     /locus_tag="VQ526_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2555395.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2079..3629
                     /gene="dltA"
                     /locus_tag="VQ526_001696"
     CDS             2079..3629
                     /gene="dltA"
                     /locus_tag="VQ526_001696"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2555396.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKYGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3626..4870
                     /gene="dltB"
                     /locus_tag="VQ526_001697"
     CDS             3626..4870
                     /gene="dltB"
                     /locus_tag="VQ526_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2555397.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4884..5123
                     /gene="dltC"
                     /locus_tag="VQ526_001698"
     CDS             4884..5123
                     /gene="dltC"
                     /locus_tag="VQ526_001698"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2555398.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5116..6384
                     /gene="dltD"
                     /locus_tag="VQ526_001699"
     CDS             5116..6384
                     /gene="dltD"
                     /locus_tag="VQ526_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2555399.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6539..6979
                     /gene="adcR"
                     /locus_tag="VQ526_001700"
     CDS             6539..6979
                     /gene="adcR"
                     /locus_tag="VQ526_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2555400.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6979..7683
                     /locus_tag="VQ526_001701"
     CDS             6979..7683
                     /locus_tag="VQ526_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2555401.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7676..8482
                     /locus_tag="VQ526_001702"
     CDS             7676..8482
                     /locus_tag="VQ526_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2555402.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8492..9997
                     /gene="adcA"
                     /locus_tag="VQ526_001703"
     CDS             8492..9997
                     /gene="adcA"
                     /locus_tag="VQ526_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2555403.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT         2927 a         1913 c         2174 g         3170 t
ORIGIN      
        1 gtacggtggt gtgagagggg ctagagatta tcccctactc gattaactcc cctgaaattt
       61 attttaatta tgcaaatttc acgtattttt gatgctgaga cgacgatctg ggaacttttc
      121 agatattttt ttgactatct aaaatctatc attagaaaag cttagagcgc caaaggattt
      181 gagcgttttt ctgattttta agactttttc ccagtctctt tttcgattga agatgtaatt
      241 attctactaa ctaactaact tcttagtact agccaacaac gataatcata attcctccta
      301 aaattaggaa taataaaggc aatagttttt gttttttcat gtaaaaaacc tcacttttgt
      361 tttctgctat tttatgctaa aatattaaaa atcaaattta attccaaagt ttgtaactaa
      421 agggggagcg ctacatgtct aattcatttg tcaagttgtt agtctctcaa ttatttgcaa
      481 atttagcaga tattttcttt agagtaacaa tcattgctaa catatacatt atttcaaaat
      541 cagtaattgc cacatcacta gttcctatct taataggaat atcctctttt gttgcgagtc
      601 ttttagttcc gttggttact aaaaggttag cgctaaatag ggttttatct ttatttcaat
      661 ttggaaagac tatattattg gcgatactgg taggaatgtt taccgtaatg caatccgtag
      721 cgcctttggt gacctatcta tttgttgttg caatttccat actagatggt tttgcagcac
      781 ccgtttccta tgctattgtg ccacgctatg cgaccgattt gggtaaggct aattcagcct
      841 tatcaatgac tggtgaagct gttcaattga taggttgggg attaggtgga ctcttgtttg
      901 caacaattgg tctgttacct accacgttta tcattttagt cttgtatatc atttctagct
      961 ttctgatgtt atttcttcct aacgctgaag tggaggtgtt agagtcagaa actaatcttg
     1021 aaattttgct caaaggttgg aagttagttg ctagaaatcc tagattaaga ttttttgtat
     1081 cagcaaattt attggaaact ttttcaaata cgatttgggt ttcttccatt atacttgttt
     1141 ttgtaacgga gttattaaat aaaacggaaa gttactgggg atattctaat acagcatact
     1201 ctattggtat tataattagt ggcttaattg cttttaggct atctgaaaag ttccttgctg
     1261 ctaaatggga aagtattctt tttcctcttg tagctatggc aatagtgaca ctgactattc
     1321 tatattttcc aaacgcacag atgtttttat tattttcagc tttagttggt atgttatcgc
     1381 aactaaaaga agttcctgaa agtgtatttc ttcaggaaac agtagaggaa aatcatttag
     1441 ttaatgtcta ttccgttctt gaagtgattt ctacattagc attttcagta tttgttttgc
     1501 taatgagcta tattactgag agttttggta ttagcatcag tttttggcta tcagccattt
     1561 gtctgatgat agaagctatt ttaatttata tcagacgaga ttattttaaa tgagagagtg
     1621 gttaaaaagt ctttaaaaat cagaaaagcg catagtatca ggtgttgaaa aaacttgata
     1681 ttgtgtgttt tattatggga agatttactt cattgtctcc tgaaatggag ttttgaagca
     1741 ggatcactct atttgaaaat atacatagaa aaactgtata atccttaaaa taagctgaaa
     1801 tctattggaa acatacggta aacaattgaa aagatactat ataaagtggt acaatggtag
     1861 aaaaataagc tagtaagaat cactcttatt ttagcgaaaa attacagaaa tgaatggttt
     1921 ttcttgatga aacagagaaa agaattgtac ctctttcttg gtcggacagc cttgtatttt
     1981 cttatctttc tagggctgct ttacttcttt agctatcttg gtcagggtca aggaagcttt
     2041 atctataatg aattttaact tgaaggagaa tccctattgt gtcaaataaa ccaatagcag
     2101 atatgattga aaccattgag cattttgctc agacacagcc tagctatcct gtctataatg
     2161 ttttggggca ggaacacact tatggcgatt taaaggctga ttcggatagt ttggctgcag
     2221 tcattgacca actaggcttg cctgagaagt ctcctgtggt tgtttttggt ggccaagaat
     2281 atgaaatgtt ggcaaccttt gtagcgctga ctaagtcagg tcatgcctac attccaattg
     2341 atagccattc ggccttggag cgagtttcag ctattttaga agtagcagag ccaagcttga
     2401 ttattgccat ttcagccttt cccttggagc aggtttctac accaatgata aatctagctc
     2461 aggttcaaga agcctttgcc caagggaaca actatgaaat cacgcatcca gtcaagggag
     2521 atgataatta ctacattatc tttacttctg gtacgactgg taagcctaag ggagtgcaga
     2581 tttcacatga taatctcctc agctttacaa actggatgat tacggataag gaatttgcga
     2641 caccgagtcg tccgcaaatg ctggcacagc caccttattc ttttgacttg tctgtcatgt
     2701 attgggcacc gaccttggca cttggtggta cgcttttcac tcttccttca gtcatcactc
     2761 aggactttaa gcaactcttt gcggctatct tttcattgcc aatcgctatc tggacatcaa
     2821 caccatcctt tgcagatatg gccatgttgt ctgaatactt caacagtgag aaaatgcctg
     2881 gaatcacgca tttctacttt gatggtgaag aattgacggt caaaacagct caaaaactgc
     2941 gcgagcgttt cccaaatgcc cgtatcatca atgcttacgg cccaacagaa gcgacagtag
     3001 ctctgtcagc agttgccgtg acagacgaga tgttagcgac tctcaaacgc ctaccaatcg
     3061 gctataccaa ggctgattct ccaaccttta tcattgacga ggaaggaaat aaactgccaa
     3121 atggcgaaca gggagaaatc attgtttctg ggccagctgt ttcaaaaggt tatatgaaaa
     3181 atcctgaaaa aacagcagaa gccttctttg agtttgaagg tctgccagcc tatcacacag
     3241 gcgatgtggg aaccatgaca gatgagggct tgcttctcta cggcggacgc atggacttcc
     3301 agattaagtt taacggttac cgcattgagt tagaagatgt ctctcaaaac ctcaacaagt
     3361 ctcgctttat cgaatctgct gtcgcagtac cgcgctataa caaggaccac aaggtacaaa
     3421 atctattggc ttatgtcatc ttaaaatacg gtgttcgtga gcagtttgag cgagatatcg
     3481 atattaccaa ggccatcaag gaagacctga cagacatcat gatgtcctat atgatgccat
     3541 ctaaattcct ttaccgagac agtttgccac taactccaaa tggaaagatt gacatcaaag
     3601 gattgattaa cgaggtgaat aagagatgat ggagtttttt caacagcttc ctcatttaga
     3661 gccatatggc aatcctcagt attttgttta tgtgattgct gcaaccttgc ccatctttat
     3721 aggtctcttt ttcaagaaac gctttgcctg gtatgaagtg ttggtaagtc tcttctttat
     3781 tgtcaccatg ttggtgggtg gaaagaccaa tcaactagct gccttgggta tttacctttg
     3841 ctgggaaata ttgctcctgc ttttctacaa gcattatcga aaaagcaagg atggcaagtg
     3901 ggtcttctac ttagttagtt ttctgtccct acttccgatt atctttgtca aggtgcaacc
     3961 agctatcaat ggaacgcagt ctttgcttgg gttcttggga atttcttacc tgacctttcg
     4021 ttcggttggg attatcatcg agctgagaga tggagtgatt aaggatttta ccctctggga
     4081 attcctccgt ttccttctct tcatgccaac tttctcgagt ggtccaatcg atcgctttaa
     4141 gcgatttaat gaaaattatc aggctattcc tgagcgagat gagttgatgg atatgctgga
     4201 tgaatctgtc cgctatatca tgtggggctt tttgtataag tttatcctag ctcatgtttt
     4261 aggagagacc ttactacctc ctctgaagaa tttagccttg cagtcaggtg gcttctttaa
     4321 tctctatgcc ttggcagtta tgtatacttt tggtctggag ctcttctttg actttgcagg
     4381 ttattctatg tttgctttgg ccatctcaaa cttgatggga atccgtagcc ctatcaactt
     4441 taacaagccc tttttatcaa gggatttaaa ggagttttgg aatcgctggc atatgagtct
     4501 gtccttctgg ttccgtgact ttgtctttat gcgaatggtg atggtgttaa ccagaaagaa
     4561 agtctttaaa aatcgtaatg taacctcaag catggcctac attgtaaata tgctgattat
     4621 gggattttgg catggtgtga cctggtacta tatcgcctat ggactctttc atggactagg
     4681 cttggtcatc aatgatgcct gggttcgcaa gaaaaaaacg ctcaataagg aacggaaaaa
     4741 agcagggaag gctgccctac ctgagaatcg ctggattcag ttgcttggca tggttgtcac
     4801 tttccatgtt gtcatgttgt cattcttaat cttttctgga ttcttgaata atctatggtt
     4861 taaaaaataa aaggaaataa aatatggata tcaaatcaga agttattgaa attattgatg
     4921 agttgtttat ggaagatgtt tctgacatga tggatgaaga tctttttgat gcaggtgtct
     4981 tggatagtat gggaacggtt gagttgattg tggagattga gaaccgtttt gacattcgtg
     5041 tccctgtaac agagtttggt cgtgacgact ggaatacagc taataaaatc atagctggta
     5101 ttgtggagct acaaaatgct taaacgctta tggatgatct tcggaccggt cttgatcgct
     5161 ggtttgttgg tttttctgct cattttcttt tatcctactg agatgcatca taatctagga
     5221 gctgaaaagc gttcagcagt ggctactact atcgatagtt ttaaggagcg aagtcaaaaa
     5281 gtcagagcac tatctgatcc aaatgtgcgt tttgttccct tctttggctc tagtgaatgg
     5341 cttcgttttg atggtgctca tcctgcggta ttagctgaga aatacaatcg ttcctaccgt
     5401 ccttatcttt taggacaggg gggagctgca tcgcttaacc aatattttgg aatgcaacag
     5461 atgttaccac agctggagaa taaacaagtt gtgtatgtta tctcacctca gtggttcagt
     5521 aaaaatggct atgatccagc agccttccag cagtatttta atggagacca gttgactagt
     5581 tttctgaaac atcaatctgg ggatcaggct agtcaatatg cagcgactcg cttactgcaa
     5641 cagttcccaa acgtagctat gaaggacctg gttcagaagt tggcaagtaa agaagaattg
     5701 tcgacagcag acaatgaaat gattgaatta ttggctcgtt ttaatgaacg ccaagcctcc
     5761 ttttttggtc agttttcggt tagaggctat gttaactacg ataagcatgt agctaagtat
     5821 ttaaaaatct tgccagacca gttttcttat caagcaatag aagatgttgt caaagcagat
     5881 gctgaaaaaa atacttccaa taatgagatg ggaatggaaa attatttcta taatgagcag
     5941 atcaagaagg atttgaagaa attaaaggat tctcagaaaa gctttaccta tctcaagtcg
     6001 ccagagtata atgacttgca gttggtttta acacagtttt ctaaatctaa ggtaaacccg
     6061 atttttatca ttccacctgt taataaaaaa tggatggact atgctggtct acgagaggat
     6121 atgtaccaac aaacggtgca gaagattcgc taccagttag aaagtcaagg ttttaccaat
     6181 atagcagatt tttctaagga cggcggggag cctttcttta tgaaggacac cattcacctt
     6241 ggttggttgg gttggttggc ttttgacaaa gcagttgatc ctttcctatc caatcccaca
     6301 ccagctccga cttaccatct gaatgagcgc tttttcagca aagattgggc gacttatgat
     6361 ggagatgtca aagaatttca atagataata taatatagaa gagttcaaga atgaaactag
     6421 ttttcacgtt ttttcttgac tctttttttg cttgtgatat aattaactgg taaacaaaat
     6481 gtcaaagaat agtatttttc tcttaaaaaa gagaagtcta aaaggaaagg agtcagatat
     6541 gagacagcta gcaaaggata tcaatgcttt tttgaatgag gtgattttgc aggcagaaaa
     6601 tcagcatgaa atcctaatag gtcattgcac tagcgaggtg gccctgacca atactcagga
     6661 gcatatcctt atgctcttgt cagaggaatc tttaacaaat tcagaattgg cccgtcgtct
     6721 caatgtcagt caggcggcag ttaccaaggc cattaagtct ttggtcaagg aagggatgtt
     6781 ggaaacatct aaagattcta aagatgcgcg tgtgattttt tatcagttga ctgacttggc
     6841 tcgtccaatc gctgaggagc accaccatca ccatgagcat acacttttaa cctatgaaca
     6901 agtggcgact cagtttactc caaatgaaca aaaagtgatt cagcggtttt tgactgcttt
     6961 agtaggagaa atcaaataat gagatatatt acggtagagg atttgtcctt ctattatgat
     7021 aaggagcctg ttcttgaaca tatcaattat tgtgttgata gtggggaatt tgttaccttg
     7081 actggggaaa atggagcggc taagacgacg ctcatcaagg ctagtcttgg aattctgcaa
     7141 ccacgcattg gaaaggtggc tatttcaaag acaaatacgc aaggtaagaa attgagaata
     7201 gcctatcttc ctcaacaaat tgccagtttt aatgctggtt ttccaagtac ggtctatgaa
     7261 tttgtcaagt cgggtcgcta tccgagaaaa ggctggttcc gtcgtttgaa tgctcatgat
     7321 gaggagcata tcaaggctag tctggactca gttggcatgt gggaacatcg agacaaacgc
     7381 ttggggtctc tatctggggg acaaaagcag cgagcggtaa ttgcgcgtat gtttgcttct
     7441 gaccctgatg tgtttatcct agacgagccg acaacgggga tggatgcagg aagtaaaaat
     7501 gaattttacg aactcatgca ccacagcgcc catcatcatg gcaaggctgt tttgatgatt
     7561 acccatgacc ctgaagaagt taaggattat gcggatcgca atattcatct agtccgtaac
     7621 caagactcgc catggcgttg tttcaacgtt catgagaatg gccaggaggt gggccatgct
     7681 tagtttatta tcttacgact ttatacaacg cgcctttttg gcggttattg ctatgagtct
     7741 tttctcaccg gtattgggaa ccttcctcat cttgcgtcgt cagagtttga tgagtgatac
     7801 ccttagccac gtctcacttt caggtgtagc ctttggtctg gttttgggga tttctccaac
     7861 tgtttctact attgccattg tcttgattgc ggcggtcttt ctggagtatc tccgtacagt
     7921 ttacaagagc tttatggaaa tcgggacagc tatcctcatg tcaacaggtc tggctgtttc
     7981 tctgattgtc atgagcaagg gtaaaagctc gagttcaatg agtttggacc aatatctctt
     8041 tggttcgatc gtgactatca gtgaagaaca ggtcatttcc ctctttgtca ttgcggcggt
     8101 tgttttgatt ttgacctttc tctttcttcg tcctatgtat atcttaactt ttgacgaaga
     8161 tacggccttt gtggatggct tgccagttcg taccatgtcc attcttttta acatggtgac
     8221 aggggtggct attgccctta tgattcctgc agcaggagct cttctggtat cgaccattat
     8281 ggtcttgcca gctagtattg ccctgcgtct ggggaaaaac tttaaatcgg ttatgctgct
     8341 tgccagtgcg attggctttt tgggaatggt agcaggactt tatatttcct actatgcaga
     8401 aacacctgca agtgcaagta ttaccattat ttttgtaact gtctttatac taatcagttt
     8461 agtaagacgt tttatcaaat aggagacgaa cgtgaaaaaa attagcttat tactagccag
     8521 tctatgtgcc ttgtttttag tggcttgttc caatcaaaaa caggcagatg gtaaactcaa
     8581 tatcgtgaca accttttacc ctgtctatga atttaccaag caagtcgcag gagatacggc
     8641 taatgtagaa ctcctaatcg gtgctgggac agaacctcat gaatacgaac catctgccaa
     8701 ggcagttgcc aaaatccaag atgcagatac cttcgtttat gaaaatgaaa acatggaaac
     8761 atgggtacct aaattgctag ataccttgga taagaaaaaa gtgaaaacca tcaaggcgac
     8821 aggcgatatg ttgctcttgc caggtggcga ggaagaagag ggagaccatg accatggaga
     8881 agaaggtcat caccatgagt ttgaccccca tgtttggtta tcaccagttc gtgccattaa
     8941 actagtagag cacatccgcg acagcttgtc agcagattat cctgataaaa aagagacctt
     9001 tgagaagaat gcagctgcct atatcgaaaa attgcaagcc ttggataagg cttacgcaga
     9061 aggcttgtct caagcaaaac aaaagagctt tgtgactcaa cacgcagcct ttaactatct
     9121 tgccttggac tatggactca aacaagtcgc aatctcagga ctttctccag atgcagagcc
     9181 atcagctgct cgcttggcag aattgacaga gtatgtcaag aaaaataaaa tcgcctatat
     9241 ctattttgaa gaaaatgcct cacaagccct tgctaacaca ctttcaaaag aagcaggtgt
     9301 caaaactgat gtcctcaatc ctttagaaag tctgacagaa gaggacacca aggctggaga
     9361 aaactacatt tccgtgatgg agaaaaacct caaggctttg aaacaaacaa cagaccaaga
     9421 aggcccagca atcgaacctg aaaaggcaga ggataccaag acagtccaaa atggttactt
     9481 cgaggatgca gctgtcaagg accgcacctt gagtgactat gcaggtaact ggcaatcagt
     9541 ttatcctttc cttgaagacg gcacgtttga ccaagtcttt gactacaagg ctaagttgac
     9601 tggtaagatg acccaggctg agtacaaggc ttactataca aaaggctatc agacagatgt
     9661 gactaagatt aacattactg ataatactat ggaatttgtt caaggtggac aaagcaagaa
     9721 atacacttac aagtatgtcg gtaagaaaat tttgacttac aagaaaggca atcgtggcgt
     9781 gcgtttcctc tttgaagcca cagatgctga cgctggacaa ttcaagtatg ttcagtttag
     9841 tgaccacaat atcgccccag ttaaggcaga acatttccat atcttctttg gaggcacaag
     9901 ccaagaagcc ctctttgaag aaatggacaa ctggccaacc tactacccag ataacctatc
     9961 tggccaagaa atcgcccaag aaatgttggc gcattgatga gaaatcgact agttcataag
    10021 agctagtcgg ttttttgata ctagatggct aacttcatta tagacctttc agaactaagg
    10081 gaacctgaaa aaataaataa aactcttgaa atatctgggc ttatatgcta gaatgtagtt
    10141 agtgtataag gttcattaga acaccaaagc ccctaactag tgcc
//
LOCUS       DAVBDG010000065        10132 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBDG010000065 DAVBDG010000000
VERSION     DAVBDG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.066"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            469..1830
                     /gene="trpE"
                     /locus_tag="VQ526_001704"
     CDS             469..1830
                     /gene="trpE"
                     /locus_tag="VQ526_001704"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2555404.1"
                     /translation="MERIIHGDALSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTSVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1827..2393
                     /locus_tag="VQ526_001705"
     CDS             1827..2393
                     /locus_tag="VQ526_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2555405.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDDRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            2404..3408
                     /gene="trpD"
                     /locus_tag="VQ526_001706"
     CDS             2404..3408
                     /gene="trpD"
                     /locus_tag="VQ526_001706"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2555406.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIARVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3405..4172
                     /gene="trpC"
                     /locus_tag="VQ526_001707"
     CDS             3405..4172
                     /gene="trpC"
                     /locus_tag="VQ526_001707"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET2555407.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            4159..4758
                     /locus_tag="VQ526_001708"
     CDS             4159..4758
                     /locus_tag="VQ526_001708"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2555408.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAV
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            4736..5959
                     /gene="trpB"
                     /locus_tag="VQ526_001709"
     CDS             4736..5959
                     /gene="trpB"
                     /locus_tag="VQ526_001709"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2555409.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            5952..6728
                     /locus_tag="VQ526_001710"
     CDS             5952..6728
                     /locus_tag="VQ526_001710"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2555410.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(6700..6801)
                     /locus_tag="VQ526_001711"
                     /pseudo
     CDS             complement(6700..6801)
                     /locus_tag="VQ526_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            7030..7170
                     /locus_tag="VQ526_001712"
     CDS             7030..7170
                     /locus_tag="VQ526_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555411.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            7207..7761
                     /locus_tag="VQ526_001713"
     CDS             7207..7761
                     /locus_tag="VQ526_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555412.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            7776..8927
                     /locus_tag="VQ526_001714"
     CDS             7776..8927
                     /locus_tag="VQ526_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2555413.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(8979..9638)
                     /locus_tag="VQ526_001715"
     CDS             complement(8979..9638)
                     /locus_tag="VQ526_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2555414.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLSIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSITELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9708..>10132
                     /locus_tag="VQ526_001716"
     CDS             9708..>10132
                     /locus_tag="VQ526_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555415.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGSRIPFLLTLQ
                     ALEEVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRK"
BASE COUNT         3160 a         1853 c         2382 g         2737 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccatac ctacgataag gcgacgttga cgcggtttga
       61 agagattttc gaagagtata aaggtgtacc gagatgaaca tctcttcatc aatgtctctg
      121 gtcgtggagg caaggaccta gctgcgattg cagattacta gaagctaaaa aataatagat
      181 ggaaaaatta cagtcttttc tctcacagag agcttgtggt tgctgaaaac aagcagagaa
      241 agctgtaaag ttgggttcat ctgaaacgag agaagaaaac atcattctca agggagtgcc
      301 ctttatcgca cagcctgtta caggaggtat ctgagacagg aatgagagat aggagaaatc
      361 ctataattga ggtggcaccg cgaatttcgt cctcacgcaa gttattttgc gtggggattt
      421 ttcatacagt cgccacgaac tagaattaga actgaaaggg aaattacaat ggaacgaatc
      481 attcatggag atgccttatc accaatcttg gcttatatgc gcttaaaggg gcaacataag
      541 gttattttag agagtattcc gagagacaag gaaacggctc gtttttctat cctagcctat
      601 aatccagttt ttgagattaa gtttgaaaat ggagttctct atcaaaatgg tcaagtgatt
      661 gatcgggatc ccttggattt cctttatgaa gtgattcata agagtcagca ccattcagag
      721 ctcccttttg gtggtggagc cattggtttt gtgggttacg atatgatttc gctttatgaa
      781 gaaattggtc aaatccctga ggatacaatt gggacgccag acatgcattt ctttgtctat
      841 gagagctata tggtctttga ccacaagaag gagaagatac atgtcataga ggatgcactt
      901 tatagtgagc gcagtcaaga ggccttggaa aaatccttga accaagtgct tgaggaatta
      961 cgcattcctg ctccaaatga atttgaagac ttggatttat caccacttga cttcaaaccg
     1021 catatcgctc cacataagtt tgagggaatg gtagaaacag ctcgtgactt gattcgtaac
     1081 ggtgatatgt tccaatgcgt actcagccag cgcttctcag cagaggttac tggaaatcca
     1141 tttgacttct acagaaatct ccgcgtgact aatccttcta attaccttta tttctatgat
     1201 tttggggatt atcaaatcat cggagccagt ccagaaagtt tggtttctgt caaaaatggc
     1261 atcgtgacaa ccaatccgat tgcagggacg cgaccaagag gggctacgga tgaagaagac
     1321 aaggccttgg cgactgacct cctctctgat gagaaggaaa cagcagagca tcgaatgtta
     1381 gtagacctgg gtcgtaatga tattggtcgc atctctgaaa cgaccagtgt ccaagtcact
     1441 aagtatatgg aagtggagct cttccgttat gttatgcatc tgaccagcgt ggtcaagggg
     1501 cgtttgcttc cagaactcac tgccatggat gccttgaaag ctacacttcc tgctggaacc
     1561 gtttctggag caccaaagat tcgtgctatg agacgtatct atgaactgga aacagaaaaa
     1621 cggggcgtat acgcaggagc aatcggctac ttgtctgcga cgggtgatat ggatttggcc
     1681 attgccatcc gaaccatgat tctcaaaaat caaagagcct atgtgcaggc tggggcaggg
     1741 attgtctacg actctatcgc ccaaaacgaa taccaagaaa ccattaacaa agcaaaatct
     1801 atgactagaa ttggagaact aagaccatga ttttattgat tgacaactat gattctttta
     1861 cctataactt ggcccaatac attgggaatt ttgcagaagt tcaggttctg agaaatgatg
     1921 attccaagct gtatgaagaa gctgaaaaag cagatggtct ggtcttttct cctggtcctg
     1981 gttggccagt tgatgctgga aagatggaag acatgattcg tgactttgca ggcaagaagc
     2041 cgattctagg gatttgttta ggccaccaag ccattgcaga agtctttggt ggtaagctag
     2101 gtttggctcc aaaagtcatg catgggaaac agagcaatat caactttgaa gcgccatctg
     2161 ttttgtatca aggtattgag gatgaccgtg cggtcatgcg ttatcacagt attttgattg
     2221 aggaaatgcc agaagacttt gaagtgacag ctcgttcgac tgatgaccaa gccatcatgg
     2281 ggattcaaca taaaaaccta ccgatttatg gcttccagta ccatccagag agcattggaa
     2341 cgccagacgg cttgtcttct attcggaatt ttatcgagaa ggttgtaaag tgaggaaact
     2401 aggatgaaag agattattga aaaactagca aaatttgaaa atttatcagg tgtggaaatg
     2461 acggatgtca ttgagcgtat cgtaactggg cgtgtaactg aagcgcagat tgcttctctc
     2521 ctcttagctc ttaagatgaa gggggaaaca cctgaagaac gcacagccat tgcccgagtc
     2581 atgagaggac atgcccagca tattccaact gagattcatg atgctatgga caactgtggt
     2641 acaggtgggg acaagtcttt cagttttaat atttccacaa ctgcagcctt tgtcttggct
     2701 ggtggcggta ttcacatggc caagcatggt aaccgctcga tttcttctaa atctggttcc
     2761 gcagatgtcc tcgaagcctt gggaatcaat cttgacctca aaccagctga actaggtaag
     2821 gtctttgata aaactggcat cgtctttctc ttcgctaaaa atatgcaccc agctatgaaa
     2881 tacatcatgc cagctcgttt ggaattggga attccaacga ttatgaactt gactggtcca
     2941 ctgattcatc caatggcttt agaaacacag cttcttggaa ttagtcgtcc agaactccta
     3001 gaaagtacag ctcaggtttt gaaaaatatg ggtcgcaaac gtgccatcgt ggttgctgga
     3061 ccagaagggt tggatgaagc tggcttgaac ggaacaacca agattgcact tcttgaaaat
     3121 ggcgaaatca gcttgtcaag ctttactcca gaggatttgg gaatggaagg ctatgctatg
     3181 gaagatattc gtggtgggaa tgctcaggaa aatgcagaaa ttttgcttag cgttctgaaa
     3241 aacgaagcaa gtccattctt ggaaacgaca gtcttgaatg ctggtcttgg tttctatgct
     3301 aatggtaaga ttgatagcat caaggaagga gttgccttgg cccgtcaagt gattgctaga
     3361 ggcaaggccc ttgaaaaact cagactgtta caggagtacc aaaaatgagt caggaatttt
     3421 tagcacgaat cttggagcag aaggcgcgtg aggtggagca gatgaagctg gagcaaatcc
     3481 agcctctgcg ccagacctat cgcttggcag aatttttgaa gaatcatcag gaccgcttgc
     3541 aggtaatcgc tgaggtcaag aaagctagcc ctagtttggg agatatcaat ctcgatgtgg
     3601 atattgtgca acaggcccag acttatgaag aaaacggagc agtgatgatt tcggtgttga
     3661 cagatgaggt tttctttaaa gggcatttgg attatctacg ggaaatttcc agtcaggtag
     3721 agattccgac gctcaacaaa gactttatca tagatgaaaa gcaaatcatc cgcgctcgca
     3781 atgcaggtgc gacagttatc ttgcttattg tggcagcctt gtccgaagaa cgcctcaagg
     3841 aactgtatga ctacgcgaca gagcttggtc tggaagtctt agtggagact cacaatctag
     3901 ctgaactaga ggtagcccac agacttggtg ctgagattat cggggtcaac aaccgcaact
     3961 tgactacctt tgaggtcgac ttgcagacca gtgtagattt agccccttac tttgaggaag
     4021 gtcgctatta catttctgaa tctgccattt tcacagggca ggatgcggaa cgactagccc
     4081 catactttaa cggaattttg gtagggacag ctcttatgca ggcagaaaat gtggcccaga
     4141 gaatcaagga gttgcagatt gacaaaggtt aaaatttgtg gactatcgac caaagaagcg
     4201 gtggaaacag ccgtttcagc aggagccgac tatatcggtt ttgtctttgc acctagtaaa
     4261 agacaggtga ctttagaaga ggcagctgtg ttggcaaagc ttattcctgc agatgtgaaa
     4321 aaggttggag tatttgtttc accaagtcgg gtagaactgc tggaagcgat tgacaaagtt
     4381 ggcttggact tggttcaagt tcacggtcag gtggcagatg atttatttga gaatttgcct
     4441 tgtgccagta ttcaggctgt gcaggtagat ggaaatgggc atgtccccaa ttctcaggca
     4501 gattatctac tctttgatgc ccctgtggca ggaagtggcc agccctttga ttggggtcaa
     4561 ctggatacga ctggactagc acagcccttc tttatcgcag gtggccttaa tgaagataat
     4621 gtagtaaaag caattcaaca ttttactccc tatgcagtag atgtatcgag cggagtggag
     4681 acagatggac aaaaagatca tgaaaagatt agaagattta tagagagggt aaagcatggc
     4741 atatcaggaa ccaaataaag atggatttta cggaaaattc ggcggacgtt ttgtcccaga
     4801 aacattgatg acagcagttt tggagttgga gaaggcctac cgtgaaagtc aggcagaccc
     4861 aagtttccaa gaggaattaa accagctttt gcgccagtac gtaggacgtg aaactcctct
     4921 ttattacgca aaaaacttga cccagcatat cggcggagcc aagatttatc tcaaacggga
     4981 agaccttaac catacaggag cccacaagat taacaatgcc ttaggacaag tttggcttgc
     5041 caaacgcatg ggtaaaaaga aaattatcgc agaaacgggt gctggtcagc acggtgtggc
     5101 aactgcaact gctgcggccc tctttaacat ggaatgtacc atctacatgg gtgaggaaga
     5161 tgtcaaacgc caagccctca atgtcttccg tatggagctt ttgggagcta aggtcgaggc
     5221 cgtgacagat ggttcgcgcg tgctcaagga tgcggtcaat gcagcccttc gttcatgggt
     5281 agctaatatc gatgataccc actatatcct tggttctgcc ttggggcctc atccatttcc
     5341 agaaatcgtt cgtgacttcc aaagtgtcat cggtcgagag gctaaacaac agtaccgcga
     5401 tatgacaggt caagatctgc cagatgccct agtagcctgt gttggtggtg ggtcgaatgc
     5461 tatcgggctc ttccatccct ttgtagaaga tgagtcagta gctatgtatg gaactgaagc
     5521 agcagggctt ggtgtggata cagagcacca cgctgctacc ttgaccaagg gtcgtccggg
     5581 tgttcttcac ggttctctca tggatgtgct ccaagatgcc catggtcaaa ttcttgaagc
     5641 cttctctatc tcagcaggtt tggactatcc tggtattggt ccagaacatt ctcactacca
     5701 cgatatcaaa cgtgccagct atgtccctgt gacagatgaa gaagccttgg aaggattcca
     5761 actcttgtct cgtgtggaag ggattatccc agccttggaa tctagccatg ctatcgcttt
     5821 tgcggtgaaa ttggccaaag aacttggacc agaaaagtct atgattgtct gcctatcagg
     5881 tcgtggggac aaggatgtgg ttcaagtcaa agaccgcttg gaagcagatg cagcaaagaa
     5941 gggagaagct catgcctaag acactaacag aaaaattgaa tgctattaaa gcagctggaa
     6001 aaggaatttt tgttccctat atcatggctg gtgaccacga gaaaggtttg gacggtcttg
     6061 ctgaaacaat tcacttttta gaagatttgg gtgtctctgc aattgaagtg ggtattccct
     6121 tttcagaccc tgttgcagat ggacctgtta tcgaagaagc tggcttgcgc agtttagccc
     6181 acgggacctc tacccaggct ttagttgaaa ccttgaaaac cattgaaaca gagattccac
     6241 tggtcatcat gacctacttc aaccccctct ttcagtacgg tgtggagaac tttgtcaaag
     6301 atctggctga tacagctgtg aagggcttga ttatcccaga cttgcctcat gaacatgcca
     6361 actttgtaga accatttttg gcagatacag atatcgcctt gattccttta gtaagcttga
     6421 ccacaggaat tgagcgccag aaagagttga ttgaaggcgc ggagggattc gtctatgccg
     6481 ttgccatcaa tggggtgaca gggaaatcag gcaattaccg tgcagatttg gacaagcact
     6541 tggcgcaact tcatcaagtg gccgacatcc cagtcttgac aggttttggt gtatctagtc
     6601 aagccgatct ggaacgcttc aatgcggtgt cagatggtgt tatcgtcggt tcgaaaatag
     6661 taaaagctct ccaccaagga gagccgattc aggattttat cagacaagca gtagcttacc
     6721 aaaaataatc aaacaagtag caagtaagag gaaaggcacg aaaggaagtc tttccttttt
     6781 cttttgcagg cgaaaggcta gttttatagt gaattgaatc tggaatatta cactacggtt
     6841 cctaaaatat tttaagaaag tgatttgaat ttcccaataa atttgttcag tttttaattg
     6901 attttactat agaagatata attttctaaa ttactctgac acagaacttt aagaaaaagc
     6961 taattattgt agtaatttct ttaagaaaat gatataattc attaaaaagt atttggagga
     7021 ggaagaatta tgttgaatca ggatctcttt gattcgcttg aggcacaaaa aattgtagat
     7081 actttgatga aagggcaaaa agattatgta gatgaacgtc tagaaaaaag agagacaatg
     7141 atagtgtcga atggttatgc atggacgtga cctaatcata ttgatactgc atttgcatca
     7201 gcagatttgt ttgagtataa attacaatta gcaggacaga cttggggata tttagaattt
     7261 gaaacaaata cagaaaaata tgggaaagta ttgttaatta taaagggtaa gaagcgactt
     7321 acgaaccaat ttcctttggt acaaaaaaat aagagtggct acttatttga atatgctcag
     7381 atgaatacac tttatcttaa tcaacattct tcctacaaaa atgatgaaga tagtcattcc
     7441 tttccaattc agatggagtt agtttctgat gaaatgattc aagaaattga acaagctact
     7501 aaaaattcga atatcgaaaa atttatgatt ttaacttatg aggcggactc agaaaacaat
     7561 attatatctg tagatgttgt tatgcctgat gcacgaactg gtcagttaca cttgattcaa
     7621 gatttgtctg agtatattca atcaagttcg taccatttcg aagaagctaa atatcaagat
     7681 attcctaatt tttcagaatt atcggaaaca gaagattttg aaattattcc aagaatagaa
     7741 aaacaagaag gtcaaaagta gtaaggagta tgtaaatgtt taatggtcgg gtattgaaag
     7801 aattacggct gttaaatggt ttaagtagag cagaattagc tcagagaatt aatttaacgg
     7861 aacaagccat ttggcagttt gagtccaacg aaacgaaacc taaattatca accaaaatgc
     7921 atttggccaa ccaatttcat gttgatttaa cttattttga acaggaagaa gagagcattc
     7981 gatttgattc ttctgtaatt gcctttagaa atgcagacct agcaacacgg aaaacaatag
     8041 atattcaaac tatgtattta cataaggtag atagtttgat tgattatttt gaaagttttg
     8101 taattatacc taatattata attcatgacc taagtaatgt agtgagtgaa tcttatcata
     8161 agggagaatc cattgaggaa ttggctcttt atgccaggga aaaattaggt atttcaaaag
     8221 ataatcatga tttgctttat aaattagaac gttcaggcat ctatatcgtg gaacgattaa
     8281 ttaatggcca agctgatgct tatagcgcat ggtcaaaatt gggaagacct tatattgtgt
     8341 tgggaacgaa taaatcatct gtacgtcgaa attttgactt agctcatgag ctaggacata
     8401 ttcttttaca taaatataaa gatatgaatg aagatggcga tcgtttggag caagaagcaa
     8461 attattttgc atcatgtttt ttattgccaa aagaagagtt tttagtcaaa tttgaagaga
     8521 gggttggcaa gcgtgtcagc aatcctgata gttatatttt attgaagtcg gatttgaatg
     8581 tttcgataca ggctttagag tatcgagctt ttaagttagg attattgact ccaaagcaac
     8641 attcttactt ttatcgtcaa attgcgcaaa aaggttacaa aatgattgaa cctttggatg
     8701 atcaaatttt tgttaaaaaa ccaagcaaag taaagagtat tctggacgtc gttttgagta
     8761 atcatctagt cagtctagcg actataatgt ctaaacaaag tattcgttta cagtttataa
     8821 gcgaaatatt ttcagtcgaa atgaaatttt ttgatcagta tcaagaagat agaagaacag
     8881 atcgatttga taacatcatc cctttgtaca aaagaaataa tttataattt aactgtgaaa
     8941 taatttcatg aaggacatat ggcagaaatt ggactttatc aaacaagcag tagcttacca
     9001 aaaataatca aacaagtagc aagtaagagg aaaggcacga aaggaagtct ttcctttttc
     9061 ttttgcagga gaaaggctag gatacccgtc gcagaagcaa actgaatcaa gatcagtaac
     9121 tccgttatgc taaagacgag agcacaagaa gctaaaaaga ggaaatcccc tgcgcccatg
     9181 cggatatcga taaaatgagc caaaattcca agagcaagga aggagaccat gaccagattc
     9241 cagccagagg aagctattag gattaggtgg aaagtcatcc agaccagtaa gggatattcc
     9301 tgatggcgaa agtcatagat gctcaaggtc aaaccagcgg tgattaggac gacttgcccc
     9361 aaggaaagca attcccaaga gtaaagcaga aagaggagtc ctaagcctag ttcaaagagg
     9421 gcataccaga caggatagcg gaccttgcag tagcgacagc gaaagcgatt gaagacctgt
     9481 gagagaatcg gaatcaaatc taagggacgc aagggagtct gacaggaatc gcagtgactg
     9541 gctgaactga taatggattg ctctggaaaa cggtcaatga ccaaaccaag aaaggaagcg
     9601 agaatgctcc cgacgagaaa aaaataaaaa tcaatcatac ttatctattc gtaaaaaatg
     9661 ggagaaatag tataatggag aaacatagat aagatggtga ggaaaagatg acaattcgtt
     9721 ttgaagaaaa ggtgagtata gaaaacgctc aatttgtatg ccaatggtcc aactccctag
     9781 gcaaatcctt tcaagaacaa tggatgggat caaggattcc ttttctactg acccttcaag
     9841 ccttggaaga agtcttttca atctttgatg aacaagagtt tgtgggactt atccagaaaa
     9901 tcaggctaga agacagcaat cttcatatcg ggagattttt tatcaacccc cagaaacagg
     9961 ggcaaggctt aggtagccag gctttaagga aatttgttag tttggccttt gaaaatgaag
    10021 atatagatag tatttctcta aatgtcttcg aggcaaatca aagagctcag aatctttacc
    10081 aaaaagaagg atttgaaatc gttcaaatgg ttgaagcacc tgtacgaaaa ta
//
LOCUS       DAVBDG010000066         9481 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBDG010000066 DAVBDG010000000
VERSION     DAVBDG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.067"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..589)
                     /gene="cbpD"
                     /locus_tag="VQ526_001717"
     CDS             complement(<1..589)
                     /gene="cbpD"
                     /locus_tag="VQ526_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2555416.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDSGSVGNSQSSASTGGTHYFK"
     gene            complement(678..944)
                     /locus_tag="VQ526_001718"
     CDS             complement(678..944)
                     /locus_tag="VQ526_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555417.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(946..2298)
                     /gene="dnaB"
                     /locus_tag="VQ526_001719"
     CDS             complement(946..2298)
                     /gene="dnaB"
                     /locus_tag="VQ526_001719"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2555418.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(2342..2794)
                     /gene="rplI"
                     /locus_tag="VQ526_001720"
     CDS             complement(2342..2794)
                     /gene="rplI"
                     /locus_tag="VQ526_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2555419.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(2791..4764)
                     /locus_tag="VQ526_001721"
     CDS             complement(2791..4764)
                     /locus_tag="VQ526_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2555420.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(4900..5448)
                     /gene="raiA"
                     /locus_tag="VQ526_001722"
     CDS             complement(4900..5448)
                     /gene="raiA"
                     /locus_tag="VQ526_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2555421.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(5528..6190)
                     /locus_tag="VQ526_001723"
     CDS             complement(5528..6190)
                     /locus_tag="VQ526_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2555422.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(6187..7485)
                     /locus_tag="VQ526_001724"
     CDS             complement(6187..7485)
                     /locus_tag="VQ526_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2555423.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            7541..8176
                     /locus_tag="VQ526_001725"
     CDS             7541..8176
                     /locus_tag="VQ526_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2555424.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            8461..9381
                     /gene="cysK"
                     /locus_tag="VQ526_001726"
     CDS             8461..9381
                     /gene="cysK"
                     /locus_tag="VQ526_001726"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2555425.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
BASE COUNT         2919 a         2114 c         1502 g         2946 t
ORIGIN      
        1 tcttaaaata atgagttccg cctgttgaag ctgaggattg actattccca acactgccac
       61 tatccaaatc tttaaaatga ataaatcctg tcatcgtgtt tgcttttata actcgtttat
      121 tataggattc tgtataacca tagttatatt cctcaatctc aatctgatct cccattacat
      181 ttgacaccca ggcaacatga ccataagttc ctgcagtaga ccaagtaatg gaaccaatcg
      241 tcggtgtatt atctacacga taaccttccc gacgagcacg atgtccccat tcattcgcat
      301 ttccataagc tgccggaatt tcaaaaccat tgacattact caaacgaaag gctacaaaag
      361 aagtacactg acgagaatac atgcgccact gatcaatctc ctggctccca tttttataat
      421 aagcaggata atcatcccca cgcgcaatcg atccatttcc tctggaatag gcatagacac
      481 tatcaccagc tgctagcatc aatcctacta ctaaaaaagg aacaagcttt ttaactgaca
      541 tcttcaagta ataacttgtt cctcttgcta taaacggtaa aattttcatt cttcctcctt
      601 aaaaaataat ataaagcgat gattgccttc acctaaatta ttcggagaaa aaagaaaaag
      661 tgagaaaatt tctcacttta gtcaagataa tgaatcaaat tcttttctgt aagaatatct
      721 gagtaagtaa aggattcaac gtaaacatta acttgtttat ctccttccac atccccaaac
      781 tccacaataa atagggatgg ataaacttca attagcttac ccaatctatt tttttggcgc
      841 ttacgaccat tctccaaagt catttctacg acttgtccct catgtgcctt gatttcttct
      901 ttgatttttt tcatcttggc tacatctgta aatgcatctg acatcttatg cctccctctt
      961 tgagatactt gaaaatttat tgtattcttt ttggaaaatc aattccactg ttccacgagc
     1021 tccactacgg tttttctcga taataacttc caccttatta tttgggatac cctcctcttc
     1081 ttcaccacca cgttcatagt agtcatcgcg atagagaaaa gctacgatat cagcgtcctg
     1141 ctcaatagac ccagattcac gaatatcaga caagaccggt ctcttgtcct gacgttgttc
     1201 tacaccacga gaaagctgac tcagagcgat tactggaacc ttcaattcct tggctagtat
     1261 tttcaactga cgagaaattt cagaaacttc ttgttgacga ttttctcgac cagttcccgt
     1321 gataagttgc aaatagtcta tcaaaatcaa accaagattt ccagtttctt gagccaattt
     1381 acgagaacga gaacgaatct ctgtaatccg aatacctggc gtatcatcga tatagatact
     1441 ggcgttagct agattaccct gagcaatagt atatttttgc cactcctcat ctgtcaattg
     1501 ccctgtacgg atagaatgtg actccaccaa gccttctgca gctaacatac ggtctaccaa
     1561 gctttccgca cccatttcga gtgaaaaaat agcaaccgtt ttgtccaact tagtcccaat
     1621 attctgagcg atattcaagg caaatgctgt cttaccaact gctggacgag ctgctaagat
     1681 aatcaactcc tcctcatgaa gtcctgttgt catatgatcc aaatcacgat aacctgtcgc
     1741 aatacctgta atatcggtcg tttgttgcga gcgagcttcc agatttccaa agttgagatt
     1801 caacacatct cgaatgttct taaacccgct tcgatttgca ttttcactga catcaatcaa
     1861 ccctttttct gcctgagcaa taatttcatc agctggttgt gacgcttcgt aagcttggtt
     1921 gacagactct gtcaacttgg caattaaacg acgtagcatt gctttttctg caacaatctt
     1981 agcataatac tccgcattag cagaagttgg cacagaatta acaatctcaa ccaagtaaga
     2041 caagccacca atattctgta aatcaccttg attatcaagg atagtacgaa ccgttgttgc
     2101 atctatggca tcaccacgat cggataaatc gaccatggct tggaaaatca aacgatgggc
     2161 atacttaaaa aagtcccgag actcaatgta ttctcgcaca aaaacaagtt tactctcatc
     2221 aataaagata gcccctaaaa cggattgctc agctaagata tcttgaggtt gtactcgtaa
     2281 ctcttctact tctgccatca gacttccctt ccttttacaa tcttgtcaag aaggtgtaaa
     2341 cttatccttc tttcacacga agattgatta cacttgtgat atcttgatag attttcactg
     2401 gcacatcaat caaaccaacc gctcgaatcg gagcttgtac ttgaatatga cgtttatcaa
     2461 tcttaattcc aaattgcttt tgcaattctt ctgcaatctt cttattggta atagaaccaa
     2521 aggtacgacc atctggacca actttttcaa caaattctac aacagtttct tctgcttcaa
     2581 gttgtgcttt aattgctttt ccttctgcaa tcatctcagc gtgagctttt tcttccgatt
     2641 tttgtttacc acgaagttca cctacagctt gagcagtcgc ttctttggct agattctttt
     2701 tgataagaaa gttttgcgca taccctgttg gtacttcctt aatttcgcct tttttacctt
     2761 ttcctttaac atctgctaaa aagattactt tcattcttct ttctcctttt cctttatttc
     2821 atttaataca atttctgtca gtttttcacc tgcttctgac aaggttacat ctttaatttg
     2881 agctgctgcc aaattaaagt ggcctccacc gcctaactct tccataatcc gttgtacatt
     2941 cagtttacta cgacttcgag ctgagataga gataaatcct tgtgtattct tcgcaagaac
     3001 aaaactcgct tcaatacctg acatggctaa catggcatct gctgccttac taataacaac
     3061 tgtatcatag catttcgtgt ccttagcctc tgctattagt acatctgaac ctaatttaca
     3121 cccctgtaaa ataagttcat taacctcacg atattcttca aaatctgtcg cagcaatttc
     3181 ctggatagca atactatcac ttccgcgcgt tctgagatag ctagcaacat caaatgtccg
     3241 actagttact cgcgaggtga aatttttagt atccaacatc ataccagcca tcaaaacact
     3301 tgcttgcata cgactcaaac gatttttctt agaattctgg aactgaatca attccgttac
     3361 caactcactg gcactacttg caccactttc gatataagta atgactgcat tatctggaaa
     3421 atcctgatcc cttctatggt ggtcaataac aatggtttgg gtaaataaat cataaaattc
     3481 ttttgataat gttaaggctg tctttgaatg gtctacaaga atcaacaaag aacgattggt
     3541 caccatcccc attgcatcct taacagacaa caacttcgta actccttctt tttctatgaa
     3601 tgaaacagct cgttcaatat ctggagacat ttgttcttca tcataaagag catagctatt
     3661 ttcaatcaca ttgctggcga acaactgcat acctacagca gagcccaaag catccatgtc
     3721 taaatttttg tgaccgacta caaaaacctg atctacactc cgaatcttat ctgaaatagc
     3781 tgtcatcata gcgcgcgtac gagtccgtgt acgcttgatt gaagcagcag acccaccacc
     3841 aaaataaact ggatttttcg tttcgtcgtt ttccttaaca accacctggt cgccaccacg
     3901 tacttcagcc aagttcaaat tgagcaaagc aactttccct atctcatcat gatttccatc
     3961 gccataagaa aatcccatac ttaaagtcaa gggcaactgt ctctgtttcg actcttctct
     4021 gaaagcatca ataacagaaa atttatcatt catcaagccc tcaagcaccg tgtagtcagt
     4081 aaatagataa aatcgatcca tacttacccg acgagaaaac atcatgtatt tttctgaaaa
     4141 ctctgatata aaattagcta caaaactatt gatttgacta atatctgact cagaagttgc
     4201 atcctccaaa tcatcataat tatccacaga gacaatccca atcactggtc gacttgttac
     4261 caattcatct gttatggctt gttccctgga tacatctaca aaatacaaaa caccggaaga
     4321 agcatccata tgaacagcat aacgcttctc accaagcttg gcataagtag acggatttcc
     4381 tactgaagcc ttgataatcg tttgaacagc ttctaaatca aaatcaccat cttccttggt
     4441 caaaatcaat tcagcatagg gattaaacca ctcaacctct ccagaagata aattcaattt
     4501 cataacacct acaggcatct gttccaatag agctgtcaaa ctttcttccg cttggtggtt
     4561 tacatactgt atctgttcta catcactcct tgtataatgc actctcagtt tcttaaataa
     4621 aaaaacatag cctcctacaa aaagaaacaa aattaaaacc gtcaacagat tattattaac
     4681 aaaaataatg aaagtggata agactccaaa cgcaatcaat cctactagaa taggaaaaat
     4741 tggacttaca taaaattttt tcattcaaaa cctcttggca cccattatac cataataccc
     4801 ctcaaaaagc gactttttaa aagtgtaatc agtaattcta tcaattataa gaaaaaggta
     4861 gtttacaatt cagtaaacct acctttacac atattgaaat taagattctt taacctctaa
     4921 caaaccaatt tcgccatcct cacgacgata aatcacattg gttgtctgat cttcaacatc
     4981 cacatagata aagaaatcat gccccaataa atccatttgt agaattgctt cttccaaatc
     5041 cattggtttt aaatcaattt gttttgaacg aacaacttta gactggacaa tatttgaatc
     5101 ttccaccaaa gcatctgtaa ataattgacc agttgctacc ttatttttat ttttacgctc
     5161 gatttttgtt ttatttttac gaatctgacg ttcaatttta tcagttacaa ggtcaattga
     5221 accatacata tcttgagata catcttctgc gcggagagta atagatccaa gcggaatcgt
     5281 tacttccact ttagccgttt tttcacgata aacttttaag ttaattcggg catccaactc
     5341 ttgttctggt tggaagtact tttcgatctt ttcgagttta gaaactacat aatcacgaat
     5401 tgcttctgtt acttctaggt tttcaccacg gatactatat ttaatcatat gagtaccttc
     5461 tttctaaaca tttttgtttt tatgatttta ttataacgct ttcattctat ttttgcaaat
     5521 tttttcctca tcttacaagg gaaaatgttt ttacatcctt agcaccggct tcttccaaca
     5581 gtttcttaac acgatttata gttgctcctg tagtatagat atcatctata agtaggattt
     5641 ttttaggaat agtgactcca cttttaataa agaaaggaag ttctgtcccc aagcgctctg
     5701 aacgattttt agaagaactg gctctctctt ctcttttctc taataaatct agatactcaa
     5761 agcctgctgc ctctaccaag ccctcaacct gattaaatcc tctattagca tatctatcag
     5821 gacttagggg aattacaaca aattgatact ctttgtactt tttcaactcc tcacttaaaa
     5881 atgaagcgaa aacttttctt aacaggaagt ctccatcaaa cttataccga ctgaaaaaat
     5941 ccttcatagc ttgattgtaa gtaaaaatcg ctctatgact gactccaact ccttctttac
     6001 accaaagttg acaatcttga cactttgttg acaactctgt tttcatacaa tttggacagt
     6061 tctcttcccc aattctttca aaagtagaat cacagtctga acaaagacaa gagtcatcat
     6121 tcctcagaag taagagacta ctaaaagtta aaacagtctt catagtctgc ccacataaca
     6181 agcacttcat agaccagcct ccttattcat catctgaatt tccttaatcg ccttcttgat
     6241 tgaagcattt aacccatcat ggaagaaaag caaatctcct gtcggtctat ccatgcttcg
     6301 tccaactcgt ccaccaatct gaatcaaact agacttggta aacaaacgat gattggcctc
     6361 tactacgaaa acatccacac aagggaaggt aactccacgc tccaagattg tcgtactgat
     6421 aagtattgtc agttctccat ctcgaaaagc ttgtacttgc tctaatcgat cctctgttac
     6481 agaagataca aagccaattt tctcatttgg aaattgctcc tgtaagattt ctgctaactg
     6541 ctcccctttc ttaatttctg aagcaaaaat gagtaacgga taagctgtct ttctctgctt
     6601 ctcaatatag gactttaact ttggtgacaa acgattcttg tctaagtagc gattaaaatc
     6661 cgataaccaa attggttttg gaataatcaa cggatttcca tgaaaccgtc tcggtaaatt
     6721 cagtcttttt agttctccta aacggacctt tttatctaac tcattggtcg aagtcgctgt
     6781 taaaaagatt ctcaatccat tctcctttac actattcttg acagcgtggt aaagcatggg
     6841 attatcaaca taaggaaaag catctacttc atccactatc agcaaatcaa aagcttgata
     6901 aaacttcaat aactgatggg ttgttgcaac aactagtggt gttcgaaaat aaggttccga
     6961 ttctccatgt agcaaagcta ttccgcaaga aaaatcctgt tgcaggcgct tgtacagctc
     7021 caaacaaaca tctatgcgag gactagccaa acacactgca ccacccgcat tgatcacttt
     7081 agccactact tgataaatca tttctgtctt tccagctcct gttaccgcat gaactaaggt
     7141 tggcttttgc ttgtctactg cttgaagcag tccctctgac accttctctt gaaaaggagt
     7201 taattggccg cgccatttga gaacatcttg cttcggaaaa tcctcctgcg gaaaatagta
     7261 taaagtttga tcacttctga ctcgcttcat cagcaagcac tctcgacaat agtaagcacc
     7321 gatgggcaaa taccattctt ctagaatagt actattacaa cgttgacaga aaagtttccc
     7381 cttctccttt ctcattgctg gaagtttctc cgccaactga cgttcttctt ctgttaattc
     7441 attctcagta aataaacgac cgagataatc taaatttact ttcatacttc tttattcgta
     7501 aaaactagca ctttagatga ttttttagta caattaaatc atggaattta ggacaattaa
     7561 agaggacggc caagtccaag aagaaatcaa aaaatctcgc tttatctgcc atgccaagcg
     7621 tgtttatagc gaagaagagg ctcgtgactt cattactgcc atcaaaaaag aacactacaa
     7681 agcgacacat aactgctctg cctttattat tggagaacgt agtgaaatta aacgtacaag
     7741 tgatgatggt gagcctagtg gtactgctgg tgttcccatg cttggggtac tagaaaatca
     7801 caatctcacc aatgtctgtg tggtcgtgac acgctacttt ggtggtatta aactaggcgc
     7861 tggaggacta attcgtgctt acgccggcag tgtcgcctta gctgtcaaag aaattggtat
     7921 tattgaaata aaagaacagg ctggcattgc tattcaaatg tcttatgctc agtaccaaga
     7981 gtacagtaac ttccttaaag aacatgatct catggagctg gatacaaact ttacagatca
     8041 agtcgatacg atgatttatg ttgataaaga agaaaaagaa actattaaag ctgcacttgt
     8101 ggagtttttt aatggaaaag tcactttaac tgaccaaggt ttacgagagg ttgaagttcc
     8161 tgtaaactta gtgtaaacaa tgaataatac agcgtttcgt tgacattctc acaactactt
     8221 tagcgagcaa aataaaaaga tgcgtaccaa aatatactag aaaatgaagc aattcaaacg
     8281 aaacctgata tcgttttcct tcacacctat ttactagaat taactgaacg caatcacttg
     8341 aaaattaatg actttgatct atgatatata gaaatcctat cgcttcgata ggatttctat
     8401 attttacaaa tggtatggat agcgttatac taaagatatc ttatacaaag aggtattcat
     8461 atgtctattt ataacaacat tactgaatta atcggtcaaa caccgattgt taaacttaac
     8521 aacatcgtgc cagaaggtgc tgcagacgtc tatataaagc ttgaagcatt taatcctggt
     8581 tcatctgtaa aagaccgtat tgcccttagc atgattgaaa aagctgaaca agatggtatt
     8641 ctgaaacttg gttctactat tgttgaagca acaagtggaa acaccggtat tggactttca
     8701 tgggtaggtg ctgctaaagg gtataaagtc gtcatcgtta tgcctgaaac tatgagtgta
     8761 gaacgacgta aaattatcca agcttatggt gctgaactcg tcctaactcc tggtagcgag
     8821 ggaatgaaag gtgctattgc taaggctcaa gaaatcgctg ctgaacgtga tggtttcctt
     8881 cctcttcaat ttgacaatcc agctaatcca gaagtacacg aaagaacaac aggagctgag
     8941 atactagctg ctttcggtaa agatggatta gatgcctttg ttgctggagt aggtactggt
     9001 ggaacgattt ctggtgtttc tcatgcactc aaatcagaaa attctaacat tcaagttttt
     9061 gcagtagaag cagatgaatc tgctattcta tctggtgaaa aacctggtcc tcacaaaatt
     9121 caaggtatct cagctggatt tattcctgat acacttgata ctaaagccta tgatggtatc
     9181 gttcgtgtaa catcagatga cgctcttgca ctcggacgtg aaattggtgg aaaagaaggc
     9241 ttccttgtag ggatttcctc agctgcagct atctacggag ccatcgaggt tgccaaaaaa
     9301 ttaggtacag gtaaaaaagt ccttgcccta gcaccagata acggtgaacg ttatctctct
     9361 acagcacttt atgaattgta accgtccaat aacgaagtct attgaaaaat ctccagacta
     9421 gagaactcac ggatagttcc taatctggag atttcttatt tgcacttttc ttgtacaact
     9481 t
//
LOCUS       DAVBDG010000067         9456 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBDG010000067 DAVBDG010000000
VERSION     DAVBDG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.068"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            75..485
                     /locus_tag="VQ526_001727"
     CDS             75..485
                     /locus_tag="VQ526_001727"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555426.1"
                     /translation="MQLLELSHTQGQLTDEQLFWLQCLLNLSQTNNLVKIKKIEEHKY
                     DYKTECNSVLRFLLDKTVNSEGRITSSTEFGNFVSNLSFQHGIAGLLFPLNKLYHPEL
                     DSKILSIIKKAVTIRTSHTYEYQYSLLFGGAGYL"
     gene            652..1122
                     /locus_tag="VQ526_001728"
     CDS             652..1122
                     /locus_tag="VQ526_001728"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555427.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKSNR"
     gene            1282..2514
                     /locus_tag="VQ526_001729"
     CDS             1282..2514
                     /locus_tag="VQ526_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2555428.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLKLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAYIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(2632..3651)
                     /gene="cbpC"
                     /locus_tag="VQ526_001730"
     CDS             complement(2632..3651)
                     /gene="cbpC"
                     /locus_tag="VQ526_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2555429.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPISEVAKGWTQDFHVTIDIDRSKPAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSS
                     GAMATGWYQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVR"
     gene            complement(3749..4438)
                     /locus_tag="VQ526_001731"
     CDS             complement(3749..4438)
                     /locus_tag="VQ526_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555430.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEDLEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(4450..5874)
                     /gene="gndA"
                     /locus_tag="VQ526_001732"
     CDS             complement(4450..5874)
                     /gene="gndA"
                     /locus_tag="VQ526_001732"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2555431.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(5950..7344)
                     /gene="mapZ"
                     /locus_tag="VQ526_001733"
     CDS             complement(5950..7344)
                     /gene="mapZ"
                     /locus_tag="VQ526_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2555432.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(7357..8514)
                     /locus_tag="VQ526_001734"
     CDS             complement(7357..8514)
                     /locus_tag="VQ526_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2555433.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(8599..8979)
                     /gene="rnpB"
                     /locus_tag="VQ526_001735"
     ncRNA           complement(8599..8979)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQ526_001735"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(9000..9329)
                     /gene="gpsB"
                     /locus_tag="VQ526_001736"
     CDS             complement(9000..9329)
                     /gene="gpsB"
                     /locus_tag="VQ526_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2555434.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
BASE COUNT         2706 a         2129 c         1652 g         2969 t
ORIGIN      
        1 tatttgacaa gttatcctaa aagtattatt atgttgttgt gttatagatt gattgaatct
       61 aactaaagga tcctattcaa ttactagaac tatcacatac tcaaggtcag ctcacagatg
      121 agcaactatt ttggttacaa tgtctactaa atttaagtca aacaaataat ttagtcaaaa
      181 ttaaaaaaat agaggaacat aaatatgatt acaaaacaga atgtaatagt gttctacgat
      241 ttttactaga taaaactgta aattctgaag gaaggatcac ttcttcaaca gaatttggaa
      301 atttcgtaag taatttatca ttccaacacg gaatagctgg actactgttt cctctaaata
      361 aattgtacca cccagaactg gattctaaaa tactctctat catcaagaag gcagtgacaa
      421 ttagaacgtc acacacatat gaatatcaat actcactgct atttggtggt gcaggctatc
      481 tatagttact cctacattta ttttctatca gtaaaaatca atactatcta caattagcaa
      541 acgtcaccgc taagaaatta atagagaatt atgatactct agaggaaata gactttgcat
      601 tgggaaaatc tggtgtccta ttatcattaa taaaatacta tcaagttacc aatgacaata
      661 ctcttaaaac tttcatccac aatagtatat ggggaaattt atcattattt cctacaaaga
      721 gatacagcca aagaaagcat tttagactat agctttgctc atggatattg tggaattgca
      781 tatgctttat ttgcctattc taaagtctta gaaccttcta tgttctataa tgatctccat
      841 acattccata ctgaattaaa aaaattatta gaaaaagtta cttctaatac tgaaaattta
      901 ggaaatttac aactttcttg gtgcaaagga atttccggaa taatcttata tctttgtatg
      961 tacgattgtg acggaaacaa agatattatt agtaaatatc aagaatttgt ttttaaccat
     1021 catctaaaaa tgatgacagg atattgccac ggaataacta gcttactaca aaccactgtc
     1081 tacaatcaaa acaaattact gatgaaaaaa tccaacaggt aattttagca tgttctgaac
     1141 gagatgatca cggtttactg atgtttcaag gagatagtgg taaagcagat ttgtttgact
     1201 tcggaatagg aagcatgggg gtatattggt gtctattaaa taataaattc ccattcgatg
     1261 tgcagacata aggagaaatg tatgaaatta ttttggacaa acaacatata tagacagttg
     1321 ctgctaaaca gctgtttttc atcattcggc gacagtattt tctacctcgc cattatcaat
     1381 tatgtggctc agtacaattt cgctccgcta gcgattttac tgatttccat ttcagagatg
     1441 gttcccctac tatcgcaact ctttctcggg attctaggag attttcaaga aaatagagtc
     1501 aaacacgcac tctgtattgc caaaatcaaa atcctgctct acgctatttt gacagtattt
     1561 ctcgtcttgt cgcccttttc attagtttca gtcattatga ttgtcatcat caacctcatc
     1621 tctgacacct tgagctacct gtctgcctac atgatgaacg ccctctacat cagtgtaatt
     1681 aaggacgacc tgcatgatgc catggggttc aggcagtctc tgatgagggt tgtccgcatt
     1741 gtcgccaatc tggctggcgc attccttatc aatgttataa gtattcaaac tatttccctt
     1801 atcaacactc tgacttttgt cattgccttt ttgggcctgt atgttattcg acataccttg
     1861 tatgaggttg aaaaaaggat tgaaatgtca catacagcac tgagttttaa gaaatatttt
     1921 caacatctta aacagtcgct ggctgtgcta ctgaagttaa aagataccgt catactactg
     1981 tttctgacga ccagtatgat tgccatcttg gatgtgtccc ctcggctgat tgccctccgc
     2041 ttcatccaac agacactagc acaactgagc attgggcaac tcctcgccct gctctccatc
     2101 atcatgtctt gtggagctat ccttggcaat atgaccagca gtaatctatt taaaaatatc
     2161 cgtttcacgc acctcttggt tttctgtgag atttccctat tggctctaat aactagtatc
     2221 ctttgtcaag cctatatcgt aattttcatg accagtttca tcagttctac gattatcggc
     2281 attctcagcc ctcgcctaca agcagctgtc tttgcctata tccccagtga caagatgggg
     2341 acggtgggct ctgctctgag cacagtggac attctcgccc cgtccctgct ctccctatta
     2401 gccctatcca tagcatcggg cgtttcggtg cagttagcat tgatattttt gtatcttatt
     2461 ttaattgctc ttatcttttg tcaatggtta gtcaagttca acactcataa ctaacgaaaa
     2521 agcatgtgta gatttcacat gcttttaatc tccccaatcg tcaggtcaag tacaacaaag
     2581 tcacttcttt gattaagcga gtgttctaat ataattataa gcgccctgtc attaccgaac
     2641 ccattcgcca ttatagttga cagaatagcc atctacggtc gtattcactg ccaaagcacc
     2701 tgagctataa gcatagtacc agttgccatt gacctggaac caacctgtct tcatgtctcc
     2761 attacctgca tttaggtagt accaagttga accatcttga taccaaccag ttgccatagc
     2821 tcctgatgaa cggagatagt accatttgtt cccaaggttt tgccaacctg ttttcatatc
     2881 gccatttggc tggtctaaat aataccaagt ggtaccttcc tgataccagc cagtggccat
     2941 tgctcctgat gaacggaggt agtaccagtt attacctaga tattgccatc ctgttcgcat
     3001 aatgccagtt tctgggtcta ggtagtacca tggagcaggt ttgcttctat caatgtcaat
     3061 agtaacatga aaatcttgag tccaaccctt agcaacttca ctaattggta gtggattgta
     3121 agaatcatcg ccaaaatttc ctagcttatt taaataatac caattgccgt cttcataaat
     3181 ccagcctgtc tttacagcat aattattatc aaaataataa gttctctttt cagcagatag
     3241 aggatattgt tcaccgtata cttttcctgt attttcagac gtttttgcct ctagtacttg
     3301 tttatctgtt tgttctagca aagcaccatc ttgtccaaaa tagtaccact tctcctgaag
     3361 gccaatttca ttaactggtt ggttatcgaa taaattatca cgataacctg ttccaggaat
     3421 ttctaagtat tgccaaccga caaccatctc tccaatatca ccaaaatagt aagttttacc
     3481 gtctatttta tgccaataga ttgctttatg atcatctttt acataatatt ttctattatc
     3541 cttatcaaca aatcggccac ctgtggtatt cgcaaatacc gtatttgtag ctagcaaacc
     3601 aaagaaaaag actgctagta gaacttgcat catttttttc aaaagtttca tctatatatc
     3661 ctcctaaaac gattgtagca aagacccgac tgcatgtcaa tatataaaaa ctcctcaaaa
     3721 aagcagttac acaactgcaa ctgcttatct attcttgcat ggtatatcca acaccacgca
     3781 cggttttaat gtagcttttt tggcctttaa catcaagctt gctccgtaga tagcggatat
     3841 agacatctac gatatttgtc tcggtcgcac tttcatactt ccaaacactt tccaacaatt
     3901 gctcacgagt caatactttc ttgcttccca taagtgtcgc caaaaggtca tactcacggc
     3961 gggtcagagc aatcatctct tcaccacgat aaaccgtgtg atgttcaaca tctatcctaa
     4021 gattgcggta ggtccttgga actttcatca gactgcagtg ttgatcaatg aagtcccgac
     4081 ctcggaagat cgccgaaata cgcgctacca gattttcgat aaggactggc ttataaatgt
     4141 atgaaactgc aaaacgctga acaacttcca gatcttcttg caagtcttcc caatgatcta
     4201 aaatcatgat gactgaggca ggtttagttc gactcaattt ttctgcaaaa tcctgagcca
     4261 tcatatctcc cagattaacg ttcaataaca tcaaatcata gtctgtctga agagccatgg
     4321 agagggcttt ttgcccctcc tctaccagat caacccgata ctgctctttc tggagttcca
     4381 aacttaaaaa atgagctaga tttcgttctt tctcaagtaa taaaatccgt ttccccatgg
     4441 ctgacctact tatttttcgt cataccaaga gtagtggaag gttccttctt tgtctttacg
     4501 ttggtaagtg tgagcaccaa agtagtcacg ttgtgcttgg atcaagttag ctggaaggtc
     4561 agctgaacgg tagctatcaa agtaagtaat agctgctgag aaagttggca ctggcacacc
     4621 agcttgaacc gcaagagcta cgatatcacg cactgcttgt tgatacttag cagtaacatc
     4681 caagaagtac tcgtccaaaa gaaggttggc aagatctgca tcgcggttgt aagcatctgt
     4741 aatcttttgc aagaaacgag aacggatgat acagccatca cgccagatag atgcgatatc
     4801 tgcaaatggc aagttccagt tgttttcttt agaggctaca cgcaattgag caaatccttg
     4861 tgcgtatgaa atgatttttg agaagtaaag ggcttgacgg attttttcaa tcaattcagc
     4921 cttgtctcct tcaaagttga aggcagctgg ttttggaagc accttgctag catgtacacg
     4981 ttcttccttg taagttgaaa tgtagcgtgc aaacactgac tcagtaatca gtgacaatgg
     5041 tacaccaagg tcaagagatg attggctcgt ccatttacca gttcccttgt tacctgcagc
     5101 atcaaggatg tagtctacga ttggtccatc ttggccttca tcgtctttac ggctcaagat
     5161 atcagctgtg atttcgatca agtagctgtc taattcaccc ttgttccact cagtaaagat
     5221 ttcagccata tcctctgcag aaaggcctag caagtgttgc atcaagtcat agctttctgc
     5281 gatcaattgc atatcaccgt actcaatacc attgtgaacc attttcacat agtgaccagc
     5341 tccatcagga ccgatgtaag tcacacatgg cttgccatct tctggtgctt tagctgagat
     5401 ttcttcaaga acatccgcaa ccaattcgta ggcctctttt tgtccaccag gcatgataga
     5461 aggaccttca agggcacctt tttcaccacc agaaactcca gtaccgataa agttgatacc
     5521 tgagtttgcc aattcttcat tacgacggat ggtatctttg tagaaagtat ttccaccgtc
     5581 aatcaagata tcacccttgt caaggtgtgg aagaagggct tggatagtag catctgtacc
     5641 aggtccagct tgaaccatca gcatgatacg acgaggtttt tcgattgagt ttacaaaact
     5701 ttcaacgtca tagcttggta caaagttctt ttcaggatgg caagcaatca catcttccgt
     5761 tttttcttta ctacggttgt agatagcaac tgtgtaacca cgtgattcaa tattaagggc
     5821 aaggttacga cccattacgg ccatacctac gacaccaaag ttagcttttg tcatttgaca
     5881 ctcctcttgt ttcatttgtt ttattttatc atttttactt ttgaaaagaa agaattttgt
     5941 aacgactgct tagtagtcca agtcatccgc atgaccagcg ccatttccga caaagtagcc
     6001 tgtcttacag ttaagggtaa agagataggt tccatctggc ttatagaggt tgtaataacc
     6061 attgccgtta acgatattga cacgttcaag gatatattgg tctccagtga tatagccacg
     6121 tgaacgtgaa gtcgctagaa tttgttccaa gacaccatcc gcaaaatccc aggcagagtt
     6181 attactatca tcaatagcag actgattata ggcaacacga ctggcacttc tttgaacagc
     6241 aacccctgca ctcgagagac ccatattgac ttcactgcga ctacttctag tctcatttga
     6301 actacttggt tttggctcac tagtcgtatt tgaacttgct tgactagagc ttgagctgct
     6361 agtttgactt gaacttgagc tagaagtact tgtttgctgg ctcttaccaa gactgatagc
     6421 cttatctagc actttatcaa gctccgtatt tccagtttta atatccgtaa atttagcatc
     6481 cgatttggct ttggcattgg tatccaacac accatccaca atagctggtt tttcaaattg
     6541 tgcattgaca tcttgaatag ccttgatttg cgttgctaga ctatcatatt tagatttggc
     6601 aagcgtatgt tcacgactac cttccagttt atcaagcaag gttttgagtt gactcagttt
     6661 atcaaactgg ctatttttca aagccgtttt attgctatct gtgtaaaagg cgtcataaag
     6721 tgtattaaaa tcatccacat ctgattgatt ggctgtagtt gattgagaag tttcaatttc
     6781 cttggtcgaa cgagccactt gacggtagac ataataagca ctgacacaaa taattactga
     6841 tacaagcgcc aaaacggtca aaataaagcc ttttttactt ttcttttctg agtcctcttg
     6901 atccagacga gaaaggtttt gctcctcttc cacttcttca gtcatttctg ttggattttc
     6961 atcatctagc aatagaggat ccaagccttg ctcctcatcg tccagaggca agggtagtaa
     7021 gatgtcttca gaagatggaa cttcctccga agaagcttct gacttctcta cagcctcacg
     7081 catctcttga attaaatcat ccagactctg agtttcaacg aactcctctt ttttgtattg
     7141 acgagtcgca aacttatccg cctcaatttc atctctgtgt tgcttaacat acttgtccaa
     7201 aatggaatcc tcaggcaaga ctcctgcttc cacttcttca tttttacgaa tagcttgacc
     7261 aactgttagc tcttttgctt catcaaaatc aaattgcggt tcttgacctt cttttttatg
     7321 acgatttcgt ctttttttac tcatgagtat ccctttctat tttacctctt gccgtttgac
     7381 acgctgacca aaatattgat atagatccac tttcaaggtt ccgttgtata acttacgctt
     7441 cttatctgct tggctaccat acttgctttc aaaagcttca tcactagtca ggataaattt
     7501 gctccaagtt ttcagcggtg caaatacttg ccccatctca gcatagagct tggtcacccc
     7561 tgcatcatct gacaaacgtt caccataagg cggattggaa atgattactc cattgatttt
     7621 atcggaacgt aaatcctgca cgcgcatctg cttaaaagta atgtctcctg caacaccagc
     7681 tgcctgagca ttggccttag caatttccac catgcgagca tcaatatcac agcccatgat
     7741 atccaactca agctcacgtt ctactttttt agccgcttct gtgcgcactt cttgaatcaa
     7801 gcgatcgctg atccagttcc attcctcaaa tgcaaaagag cgacgaagac ctggcgccat
     7861 ctttctagca atcataactg cctcaataca gaaagtcccc gaaccacagg tcggatcaat
     7921 caaaggcttg tctggatacc agttagaaag ttgtaaaatg gctgctgcca tattttcctt
     7981 gataggagcg ccaccttttt cggtacgata accacgttta aagaggctag acccggtcgt
     8041 atcaatcatg acagttgcca catctttgag aatagagacc tcaatcttaa actctgggcc
     8101 attctccatc agaggaaccc cttctgggcg agcatagtgt ttctgcaatt tcttgacaac
     8161 agctttctta gaaatagcct gaacactggg ctcattgtga agtttggact taacacattt
     8221 agcttttgaa atcgggaacc gagctccaag tggtaaataa ttttcccaat ctaaagcgaa
     8281 aactccctga aatagctctt caaaagtctt agctgggaac gttcctacga taattttgat
     8341 acgatctgct gcccgaagcc aaaggttggt ttcgataata gctctcacgt ctccttgaaa
     8401 acgaacacgt ccattttcaa cctgacaatc gtagcccaac tctcgcactt cacgaccaac
     8461 gacagcctca agccctgctg ccacagttgc aattaaatta aattcttttt tcatgttaca
     8521 gtcttgcaag acctttctat atgtccactt taaaaaatga ggttgagaaa atttctcagc
     8581 cccaacctat atgcaatttt ctataagcca tgttttgttc cagaagtgac taggaccact
     8641 tccttcgata atcatctgtc taccactaac agctctagct gatagcagtc agaatactac
     8701 gttcgtttcc gcgtactcca tgccccgacc aaaatttggg ttgctagctt gaggggttta
     8761 ccgcgttcca ctctctctgt ttccagaaag actccgtcac tgtggcactt tcaagcctac
     8821 tctggcctat ccaaggactt agccatttca actgccgtaa cgatttctcg tccctaggct
     8881 tatggtttcg cctagcacaa acactacagg catcacagcc tgtgctagca tggactttcc
     8941 tcatgagaag caattctcct cacgcgatta tccaaaaatt gcacatctca aataactact
     9001 taaaaatctg agttatctaa aatttgttta ccaaaaactt ctttttccaa tctattcagg
     9061 cgtttcaaaa tatcaaaatt cgtcatagaa cttgtaattg ccgcttcaag gggttctgct
     9121 tgaactggtg aaggtttcgg tttacgagtt aattcttcct tcaaatccgc aatttcctga
     9181 cgaagtgact tgaccaaggc agcataggtt tcatagtcct tgatgacatc gtctaaaaac
     9241 tcgtcaactt ctactttatt atagccacgg acttcacgtc caaactcttg ttcaaaaata
     9301 tctttcgctg aaaaaataat acttgccatg tctctctcca ttctcgcttg ctagtattat
     9361 tatataaaaa aaggcaaaca aaaatcaagg tcaaagcttc aattttcgga aaaattttca
     9421 gcaagttcat ttaattcatc aaatgttaat ctcttt
//
LOCUS       DAVBDG010000068         9332 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBDG010000068 DAVBDG010000000
VERSION     DAVBDG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.069"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..116
                     /locus_tag="VQ526_001737"
     CDS             <1..116
                     /locus_tag="VQ526_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2555435.1"
                     /translation="TPEQAKQILEYGVRGIVVGGAITRPKEITERFVAGLK"
     gene            140..1063
                     /locus_tag="VQ526_001738"
     CDS             140..1063
                     /locus_tag="VQ526_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclically-permuted mutarotase family protein"
                     /protein_id="HET2555436.1"
                     /translation="MKIDVINHLDSPGLAGMICRRINNRILVYGGSYFPNNDPLKSSK
                     VQSNKIRVYDGKFNLLFEQDGQISPDKGITIEVENEIYYILGSSIYRITIDESVEEIC
                     IGDFDFQLESGYGCYYNGHLFFGFQESYLFNIETKELFKKSDFPVSGRGQGLSVKYQN
                     ELYYLGGANNEAYLDGYKYIFEKDKWEKLEFELPSSVLGASSIQINESDLLILGGFNE
                     IAYNKAVIDLATPGYREEYFSKGRDFFNWNRSLRILDLETGKVDIIANDERFALCGAG
                     FLKTDNGYYVVSGECSPGRRTSDILLLEEEM"
     gene            1145..2773
                     /locus_tag="VQ526_001739"
     CDS             1145..2773
                     /locus_tag="VQ526_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2555437.1"
                     /translation="MKKIVKLALTAAAVLIAVVGLVACSSGSKKSDSSESGKEKVFYL
                     SGLLQGTGDRIPYVWQNGDGLVPYLLYRTLLMADSKYEKSTPDLAKDWKKSDDEKTYE
                     FTLKDGLKWSDGEALTASDVEFSVKLALKASVINGIFPSTFTKIEGAKDFKDDKADKI
                     SGLTVDGNKVTFKLTAPVGNFEQMMTQFFILPKHKLESENPLELNNSSFWTAPVTSGM
                     YKVKEISAGNFLEYDKNENYENEKPKFDKVVFNYISDPVLALQDGKSYFYSTNKPQEI
                     SQLDAVSTLTKKPIDILFYRYFIANLAGVEGNGDSLIANPKVREAILYAINRDELAKS
                     VFSGIADVNNTGVPSSLEAHLKSANKFEYNPEKAKQLLKEAKYDNSRNLILAYYYKDQ
                     ASQDFMQAVSYQLNEVGIKNEVVQITSDATTGLFKTRKYDLAYKGLSSFGYETWYGEY
                     SSTNTNFKNITNGETSFDELSKKLSETSDVKERNKILASLQKLEQEKLLKLNLFTFKN
                     FLYLNTEKVLIPDDVQFGNPFYKFDYKFEKWDAK"
     gene            2882..3832
                     /locus_tag="VQ526_001740"
     CDS             2882..3832
                     /locus_tag="VQ526_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555438.1"
                     /translation="MKKYILKKVLFMIPMLLIISFLVFIALDLTPADPLTYMVSPDMA
                     SSAEQIEKLRQQLGLNDPVLVRYGRWLWQLLHGDFGYSIVNGNPISKIVGQALPATFE
                     LAFVSLIISTIVGIVIGVISAVKQNGIIDNVARFLAVIGTAIPQFFFGILILNFFAIQ
                     LRILPIGGRFTSGDFTFINRVEHLILPVMAMSIALVAALMRYTRNSMLDVFNADYVKT
                     ARAKGVPEWKVYFKHIFRNAMRPILVLLIFRLPMLIGGSVIIESVFSWPGIGSIILSS
                     VTAGDYPVIMITTLMISVVVLFASLLVDIMAAILDPRIRY"
     gene            3849..4700
                     /locus_tag="VQ526_001741"
     CDS             3849..4700
                     /locus_tag="VQ526_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555439.1"
                     /translation="MKPMPLWKRNLKKFYNNKLAFIGFICFMLILLACILAPLLTTYS
                     PDVVDLGSMNRPPSAKHILGTDKLGRDVFARILYGGRVSIQVGMYGAICGAVIGTVLG
                     GIAGYFGGKIDALLVRLAELFLTFPNMIVILLLSSIFGQGVFNLIFVFSVMGWMTTFR
                     MVRNEFMSLKQETYVDVCRAFGFSDSRIIFNNILRNAISPVIVSLSLNVAGFILSEAG
                     LSFLGVGVPSDIPTWGNIINAAKTADVIKNSWWLWLVPGSIITLFVLSINFIGDGLRD
                     IMDPKQQ"
     gene            4713..6695
                     /locus_tag="VQ526_001742"
     CDS             4713..6695
                     /locus_tag="VQ526_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555440.1"
                     /translation="MNQTVLDVKNLKTEFITDKKAFPIIKDVNFTLERGKVLGIVGES
                     GCGKSVTVNTVLNLLPKNGRIAEGEITYFDEDKPIELQKLKQYGKEFRKLRGENISMI
                     FQDPMSALNPVYTIGNQITEVLHEHYDISKEEANKRAIEMLEKLGIHNAAERMNDYPH
                     QFSGGQRQRIVIAIAMVCNPDILIADEPTTALDVTIQAQILDLLDDLRKEHGTSIILI
                     THDLGVIAQIADEVAVMYAGEVVEVGTVAQIFDQPKHPYTRSLLRSIPNPENMDKKLH
                     VIQGSVPSISEISEKGCRFANRIPWLEKEHHEEEPQLHDLGDGHFVRCSCYKHFFFED
                     KEEETLAEKHVGNVVLEVKNIKKYYYPKKQLFKPLGSPLKALDDVSLELRKGTTIGIV
                     GESGSGKSTIAKSLMKLHEITDGEINIDLNGKTQNIYGIKRKEDLDFRKKVQMVFQDP
                     YASLNPTKKIYNSFDEPMIVHNIGNKEERFERMKESLKMVNVPVEYLERYPHEFSGGQ
                     RQRLCIARALCMKPEILILDEPVSALDLSVQAQVLNYLVEIQNKEDLTYVFISHDLGV
                     VKYMCDYLYVIHKGRIVEAGSREDIYNNPMHIYTKKLLAAIPEVDYHFKEALATKRKE
                     NEVEFKAQYHEFYDEDGRAYDLKQVSPTHFVALKPE"
     gene            6705..8114
                     /locus_tag="VQ526_001743"
     CDS             6705..8114
                     /locus_tag="VQ526_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555441.1"
                     /translation="MKLNKKIYFFDKGIFNPYRMSNVKLKVNQAVKDKEIFFTEEKPW
                     EIRFDNSYPNVFFDDLAGVYRCYYSTFTDDEESEKYSLEERKERQYLPRAKRIVSLCY
                     AESKDGVNWVKPNLGITEFRGSTENNIIGHFLHGTSVFLDKHDADENRRYKMFTKIDY
                     GNGVHFIAVAFSKDGLHFDEYIKVPNFNPRADTHNHIIYDEALNRYVLITRTWRDSLR
                     LPCVSTSADFINWTPIQEILNVCDYENQIYSMPIFKRGDYILGLASVFHEGDQLNKNY
                     DTVDLQLTYSYRHVGWHYLNTDTPLIPRGKGQYGDGEFDCGCIYSSAPVTIGDRTYFY
                     YMGGNGQHTNFRETSLSRAYIEKDHFAYWDIKRPEYPGVLYTNGFIFLNDQVYLDADI
                     AAGGFVTIELFENNHTPMEITASLEKIEDGRYQVLFSEPLPRTQTRLKISFKNAKIYA
                     IEGNLDIFRIESDNALLRG"
     gene            8119..8607
                     /locus_tag="VQ526_001744"
     CDS             8119..8607
                     /locus_tag="VQ526_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET2555442.1"
                     /translation="MKYDLYDNCIELLKEREVTIEDMAALVIFSQQKYYPELTLDDAS
                     YAIQRVLKKREVQNVIMTGIELDKLAEAQKLSPEFQKIMEKDNPLYGIDEVIVLSILN
                     LYGSIAFTNYGYLDKLKPLILERLNENHEGVCNVFLDDIVGAIAAAACSKIAHNHASD
                     EI"
     gene            8895..9302
                     /locus_tag="VQ526_001745"
     CDS             8895..9302
                     /locus_tag="VQ526_001745"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2555443.1"
                     /translation="MTVINKFVLMEQAKKVLKNAYFPYSKFPVGAAILFKDGKVITGA
                     NIENVSFGVTNCAERSAIFYGTSQGYRKGDILAIAVAGETEDYLPPCNICRQVMVEFC
                     EPDTLVFLLNGKGNILELRLEELVPYSFSSLEM"
BASE COUNT         3193 a         1318 c         1755 g         3066 t
ORIGIN      
        1 atacaccaga acaagccaaa caaatccttg aatatggagt gcgaggaatc gttgttggtg
       61 gcgctattac tagaccaaaa gagattacag aacgttttgt agcgggatta aaatagcatg
      121 ccagagtaag aggtgtaata tgaaaattga tgttataaat catttagact caccaggatt
      181 agctgggatg atttgtagac ggattaataa tagaatttta gtttatggag gtagttattt
      241 cccaaataac gatcctttaa aaagttcaaa agttcaatca aataaaatta gagtttatga
      301 cgggaaattt aatcttctat ttgaacaaga tggacagatt tctccagata aaggaataac
      361 gattgaagtt gaaaatgaaa tctattacat tctaggctcg agcatttatc gtattacgat
      421 tgatgaaagt gtagaagaaa tctgtatcgg ggattttgat ttccaactag agagtggtta
      481 tggttgttat tataatggcc acttattctt tggatttcag gaaagttatc tgttcaatat
      541 tgagactaaa gaacttttta aaaaatctga cttcccagtt tctggtagag gacaaggttt
      601 atcagtcaaa tatcaaaatg aattgtatta tttaggtgga gcgaacaatg aagcttattt
      661 agatggatac aaatatattt ttgaaaaaga taagtgggaa aaacttgagt ttgaattacc
      721 aagttcagtt ttaggagcgt caagtataca aataaatgaa agtgatttat taatccttgg
      781 tggctttaat gaaattgctt ataataaagc agttattgat ttagcaaccc ctggctatag
      841 ggaagaatac ttttctaagg gaagagattt ctttaattgg aatcgtagct tgcgaatact
      901 agatttagag acaggtaagg tcgatattat tgcaaacgat gagcgttttg ctctttgtgg
      961 ggcaggattt ttaaaaactg ataatggata ttacgttgtt agtggggaat gtagcccagg
     1021 aagacgtact tctgacatac ttttgttaga ggaggaaatg taacgaagag tttaaatttt
     1081 taattactgc aatacttact taatcacata aataattact taatacaaat aaaggagtat
     1141 taaaatgaaa aaaattgtta aattagcttt aacagctgca gcagttttaa tagctgtagt
     1201 aggattggtt gcttgtagtt cgggaagcaa aaaatctgat tcttcagagt ctggaaaaga
     1261 aaaagtattc tatttatcag gtttgcttca aggaactgga gatcgtatcc cttacgtttg
     1321 gcaaaatggt gatggtttag ttccatattt gttataccgt acactgttaa tggcagattc
     1381 aaaatatgaa aaatcaactc cagatttagc taaagattgg aaaaaatctg atgatgaaaa
     1441 aacttatgaa tttacactta aagatgggtt gaaatggtct gatggagaag ctttgacagc
     1501 ttccgatgtg gaattcagtg ttaaattagc attgaaagct tcagtaatta atggaatttt
     1561 cccttcaaca ttcacaaaaa ttgaaggagc taaagatttc aaagacgata aagcagataa
     1621 aatttctggt cttacagttg atggaaataa agtaacattt aaacttactg ctccagttgg
     1681 taactttgag caaatgatga ctcaattctt tattcttcca aaacataaat tagagtctga
     1741 aaatcctctt gaattaaata atagctcatt ttggacagct cctgttacaa gcggtatgta
     1801 caaagtaaaa gaaattagtg caggtaattt cttagagtat gacaaaaatg aaaactatga
     1861 aaatgaaaaa ccaaaatttg ataaagtagt attcaactat atttcagatc cagttttagc
     1921 attgcaagat ggtaaatcat acttctattc aacaaacaaa cctcaagaaa ttagtcagtt
     1981 ggatgctgta tctactttaa ctaaaaaacc aattgatata ctattctatc gctactttat
     2041 cgctaactta gcaggtgttg aaggtaatgg agattccttg attgctaatc ctaaagttag
     2101 agaagctatt ctatatgcaa ttaatcgtga tgaattagct aaatctgtat ttagtggaat
     2161 tgcagatgtt aataatactg gtgttccttc aagcttagaa gctcatctga aatcagcaaa
     2221 taaatttgaa tataatccag aaaaagcaaa acaacttctt aaagaagcta aatacgataa
     2281 tagcagaaat ctaattttag cttattacta taaagatcaa gcctctcaag atttcatgca
     2341 agcagtaagt tatcaattga atgaggttgg aatcaagaac gaagttgtcc aaattacttc
     2401 tgatgcaaca acaggattgt tcaaaacacg taaatatgac ctagcttata aaggattatc
     2461 atcatttggt tatgaaactt ggtatggtga atatagttca acaaatacta actttaaaaa
     2521 tattacaaat ggcgaaacaa gttttgatga gttgagcaaa aaattgtctg aaacttctga
     2581 tgtaaaagaa agaaacaaaa ttttagcaag tctacaaaaa ttagaacaag aaaaactatt
     2641 gaagttaaac ttattcacat ttaaaaactt cttgtattta aatactgaaa aagttttaat
     2701 tcctgacgat gttcaatttg gtaatccatt ctataaattt gattacaaat tcgaaaaatg
     2761 ggatgctaaa taaaatcgtt taaaattatc agtgattaag tttgataatc ttttaggagt
     2821 gtagagatgc tgtttattga atcatgtatc tctcactctt gttttttcag gagaaattaa
     2881 aatgaaaaaa tatattctaa aaaaagtatt attcatgatt cctatgttgt tgattattag
     2941 ctttttagta ttcattgcac tagatttaac accagcagat ccattgacat atatggtatc
     3001 tccagatatg gctagtagtg cagaacaaat cgaaaagcta agacagcaat taggtttaaa
     3061 tgatccagta ttagttcgct atggaagatg gctttggcaa ttactccatg gtgattttgg
     3121 atacagtatt gtaaatggta acccaatttc caaaattgta ggacaagcat tgcctgcaac
     3181 ttttgaatta gcttttgtgt cattaattat ttcaacaatt gttggtattg taattggggt
     3241 tatatcagcc gttaaacaaa atggtattat cgataatgtt gctcgttttc tagctgttat
     3301 cggaactgcg attccacaat ttttctttgg tattttaatt ttaaacttct ttgcaattca
     3361 gttaagaatt ttacctattg gtggtagatt taccagtgga gattttacat ttattaatag
     3421 agttgaacac ttaattctac cagtaatggc tatgtcaatt gcattggtag ccgctttaat
     3481 gagatacaca cgtaattcta tgttggatgt ttttaatgca gattatgtta aaacagctcg
     3541 tgcaaaaggt gttcctgaat ggaaagttta ttttaaacac atatttagaa atgctatgcg
     3601 accaatttta gtgttattga ttttccgtct accaatgtta attggtggat cagtaattat
     3661 cgagagtgtc ttttcttggc ctggaattgg ttctatcatc ctatctagtg taacagcagg
     3721 tgactatcct gtaattatga ttaccacatt aatgatttct gtcgtagtgt tatttgctag
     3781 tctacttgta gatattatgg ctgcaatatt agacccacgt attcgttatt aggaaggagg
     3841 tcaatgttat gaaacctatg cctttatgga aaagaaattt gaaaaaattc tataataaca
     3901 aattggcctt tatcggcttt atatgcttca tgctaattct attagcatgt attctagcac
     3961 ctttattgac aacctattct ccagatgttg ttgatttagg atctatgaac cgtcctcctt
     4021 cagcaaaaca cattctggga acagataaac taggtcgtga cgtatttgct cgaatcctat
     4081 atggtggtcg agtttcaatt caagtaggga tgtatggagc gatttgtgga gcggttattg
     4141 gtactgtact tggtggtatt gctggatatt ttggtgggaa aattgatgct cttctagttc
     4201 gtctagcaga attattctta acattcccga atatgattgt cattctcttg ctttcaagta
     4261 tttttggaca aggtgtattc aatcttatct tcgtattctc agtaatgggt tggatgacaa
     4321 cgttccgaat ggttcgtaat gagttcatgt ctttgaaaca agaaacatat gtggatgtat
     4381 gtagagcttt tggtttttct gacagtcgaa ttatctttaa taatatttta agaaatgcta
     4441 tctcaccagt aattgtatcg ttgtctttaa acgttgctgg tttcattctc agtgaggcag
     4501 gtcttagttt cttaggagta ggtgttcctt ctgatattcc tacttgggga aatatcataa
     4561 atgctgccaa aacagccgat gttattaaaa atagttggtg gctgtggtta gttccagggt
     4621 caatcattac tcttttcgtt ctttctatca actttattgg tgatggatta cgagatataa
     4681 tggatccgaa acaacagtag gaggttgaca aaatgaatca aacagtttta gatgtaaaaa
     4741 acttaaaaac tgaattcatc actgataaaa aagcatttcc gattattaaa gatgtaaact
     4801 tcactttgga aagagggaaa gttttaggga ttgttggtga atctggatgc ggtaagagtg
     4861 taacagtgaa tacagtgtta aacttattac ctaaaaatgg acgcattgct gaaggtgaaa
     4921 tcacatattt cgatgaagat aagccgatag agcttcaaaa attaaagcaa tatggtaaag
     4981 aatttcgaaa gttacgtggt gaaaacatat caatgatttt ccaagatcca atgtctgcat
     5041 tgaatccagt atatacaatt ggtaatcaaa ttactgaagt ccttcatgaa cattatgaca
     5101 tttctaaaga ggaggcaaat aaacgtgcta tagaaatgtt agaaaaactt ggaattcata
     5161 atgctgcaga acgcatgaat gactatccac accaatttag tggagggcag cgtcaaagaa
     5221 tagtcatagc gattgcaatg gtatgtaatc cagatattct aattgcagat gaaccaacaa
     5281 ctgcccttga tgtaacaatc caagcacaaa tacttgattt acttgatgat ttacgtaaag
     5341 agcatggaac ttctattatc ttaattactc acgatttagg cgttattgct caaattgcgg
     5401 atgaagttgc agtgatgtat gctggtgaag tcgttgaagt tggaactgta gctcagatct
     5461 ttgatcaacc taaacacccg tatactcgtt ctctattaag atctattcct aatcctgaaa
     5521 atatggataa aaagctacat gttatccagg ggagcgtacc ttcaatttct gaaatatctg
     5581 aaaaaggatg cagattcgca aatcgtattc catggttaga aaaagaacat catgaagaag
     5641 aacctcaatt gcatgattta ggtgacggac attttgtaag gtgttcttgc tataaacatt
     5701 tctttttcga agataaggaa gaagaaacat tagcggaaaa acatgtaggt aatgttgttt
     5761 tagaagtaaa aaatattaaa aagtactact atcctaagaa acaactattc aagccactag
     5821 gttctccgtt gaaagcactt gatgacgtat ctctagagtt aagaaaagga actactattg
     5881 gtatcgtagg agagtctgga agcggtaaga gtacgattgc gaaatcatta atgaaactcc
     5941 atgagattac agatggtgaa atcaatattg atttgaatgg gaaaactcaa aatatttatg
     6001 gaattaaacg taaggaagat ttggatttcc gtaagaaagt tcaaatggta ttccaagatc
     6061 cttatgcatc attgaaccca actaagaaaa tttataattc atttgatgaa ccgatgattg
     6121 tccataatat tggaaacaaa gaggaacgct ttgaacgtat gaaagaatcc ttgaagatgg
     6181 taaacgtgcc agttgagtac ttggagcgtt atcctcatga attttcaggc ggacaacgtc
     6241 aacgtttatg tattgctcgt gccctttgta tgaaaccaga aattttaatt ttagatgaac
     6301 ctgtttcagc attagatcta tctgtacaag cgcaagtttt gaattatctt gttgaaatcc
     6361 aaaataaaga agatttgact tatgtattta tttctcatga cctaggtgtt gtgaaataca
     6421 tgtgtgacta tctatatgtt attcataaag gacgtattgt agaagctggt tctcgagaag
     6481 atatctacaa taacccaatg catatttaca ctaaaaaact attagctgct attccagaag
     6541 ttgattatca cttcaaagag gcattggcaa caaaacgtaa agaaaatgaa gttgaattta
     6601 aagcacagta ccatgaattt tatgatgaag atggccgtgc ttatgatctt aagcaggttt
     6661 caccaacaca ttttgtagcg ttaaaaccag aataggtggt aaatatgaaa ttaaataaaa
     6721 aaatatattt ctttgataaa ggaattttta atccttatcg aatgagtaat gtaaaattaa
     6781 aagttaatca ggcagtaaag gataaagaaa ttttcttcac tgaggaaaaa ccatgggaaa
     6841 ttcgttttga taacagttat ccaaatgttt tctttgatga tttagcaggt gtttatcgtt
     6901 gctactattc tacatttact gatgatgagg agtctgaaaa atactcttta gaggagcgta
     6961 aggaacgtca atatctacct cgtgcgaagc gtattgtttc actttgttat gctgaaagta
     7021 aagatggtgt taactgggtg aaaccaaatc taggtattac agaatttcga ggatcaactg
     7081 aaaataatat tatcggacat tttttacatg gtacatctgt ttttctagat aaacatgacg
     7141 cagatgaaaa tcgtcgttat aaaatgttta caaagattga ttacggtaac ggtgttcatt
     7201 ttattgctgt agcattttca aaagatggtc tacactttga tgaatacatt aaagtaccaa
     7261 acttcaatcc acgtgcagac actcataacc atatcatata tgatgaagca ttaaatcgct
     7321 atgtgctaat tactcgtact tggagagatt cattgcgctt gccgtgtgta tctacgagtg
     7381 ctgatttcat taactggact ccaattcaag aaattttgaa tgtctgtgat tatgaaaatc
     7441 aaatttattc tatgcctatt tttaaacgtg gcgattatat actaggttta gcttcagttt
     7501 tccatgaagg agatcaactg aacaagaatt acgatacagt ggaccttcaa ttgacttata
     7561 gttatcgtca tgttggttgg cattatctaa acactgatac tccacttatt cctcgtggta
     7621 aaggtcaata tggtgatgga gaatttgact gtggatgtat ctattcttct gcaccagtta
     7681 ctatcggtga ccgtacttat ttctactaca tgggaggaaa tggacaacat acaaacttcc
     7741 gtgaaacaag cttgtctcgt gcttatattg agaaagatca ctttgcatat tgggatatta
     7801 aacgtccaga atatccaggt gttttgtata ccaatggatt tattttcctc aatgatcaag
     7861 tttaccttga tgcagatatt gcagctggag gctttgtaac aattgaattg ttcgagaata
     7921 atcacactcc aatggaaatt acagcttccc ttgaaaaaat agaagatggt cgctatcaag
     7981 tgttattcag cgaaccttta cctcgaactc aaactcgact gaaaattagc tttaaaaatg
     8041 ctaaaatcta cgcaatcgaa ggaaatttag atattttcag aattgaatca gacaatgctc
     8101 tattgagggg gtaagattat gaaatatgat ttatatgata attgtattga acttctaaaa
     8161 gaacgcgaag ttacaattga agatatggca gcgttagtaa tattttcaca acaaaaatat
     8221 taccctgaat taactcttga cgacgcttca tatgcaattc aacgtgtact aaaaaaacgt
     8281 gaagttcaga atgtaattat gacaggaatt gagttagata agttagctga agcacaaaaa
     8341 ctatctccag aatttcaaaa gattatggag aaagataatc cattgtatgg gattgatgaa
     8401 gtaatcgtct tatcgatatt gaatttatat ggttctattg cctttacaaa ttatggctat
     8461 ttagataaat taaaaccact gattttagaa agattgaatg agaatcacga aggtgtttgt
     8521 aatgtatttc tagatgatat tgttggtgca atagccgcag cagcatgtag taaaattgca
     8581 cacaatcatg ctagtgatga aatataacta ttgaatttag tatcgtaaat aataaagcgt
     8641 ttggtttcta gattattgtc attcaaattg gataatgata aatctagaaa ccaaatatat
     8701 tttccgcata aaatgaggga ttatatatct cactttttta caattgtata gtggattaaa
     8761 actagaatag tgcatctcta cttctaaaac attgttagaa atcgatttga ctgtcctgat
     8821 tgatttgtac tgttcttatt tcattttact ataatttagt gtgagtaaaa tataataata
     8881 aaggagttaa aacaatgaca gtaattaata aatttgtttt aatggagcaa gcaaaaaaag
     8941 ttttaaaaaa tgcttacttt ccgtattcca aatttcctgt aggtgcagca attttattta
     9001 aagatggtaa agtaattaca ggagcaaata tagaaaatgt atcgtttggt gtaactaact
     9061 gtgcagaacg tagtgctatt ttttatggga cttctcaagg atatagaaaa ggagatattc
     9121 ttgcgattgc tgttgcaggt gaaactgaag actacttacc accatgcaac atttgtcgtc
     9181 aagttatggt agaattttgt gagcctgata ccttagtctt tctattaaat ggtaagggaa
     9241 acatacttga attgcgatta gaggaattag tcccatattc gttctcaagt ctagaaatgt
     9301 aattacgtag tatgaaataa ggagaaaaaa ac
//
LOCUS       DAVBDG010000069         9123 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBDG010000069 DAVBDG010000000
VERSION     DAVBDG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.070"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(78..536)
                     /locus_tag="VQ526_001746"
     CDS             complement(78..536)
                     /locus_tag="VQ526_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2555444.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(533..973)
                     /locus_tag="VQ526_001747"
     CDS             complement(533..973)
                     /locus_tag="VQ526_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555445.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1179..3128
                     /gene="mutL"
                     /locus_tag="VQ526_001748"
     CDS             1179..3128
                     /gene="mutL"
                     /locus_tag="VQ526_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2555446.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVDQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3453..3920)
                     /locus_tag="VQ526_001749"
     CDS             complement(3453..3920)
                     /locus_tag="VQ526_001749"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2555447.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(3921..5126)
                     /locus_tag="VQ526_001750"
     CDS             complement(3921..5126)
                     /locus_tag="VQ526_001750"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2555448.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5146..5781)
                     /gene="ribE"
                     /locus_tag="VQ526_001751"
     CDS             complement(5146..5781)
                     /gene="ribE"
                     /locus_tag="VQ526_001751"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2555449.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5766..6866)
                     /gene="ribD"
                     /locus_tag="VQ526_001752"
     CDS             complement(5766..6866)
                     /gene="ribD"
                     /locus_tag="VQ526_001752"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2555450.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAIIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7000..7114)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7271..7864
                     /gene="ruvA"
                     /locus_tag="VQ526_001753"
     CDS             7271..7864
                     /gene="ruvA"
                     /locus_tag="VQ526_001753"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2555451.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            7874..8437
                     /locus_tag="VQ526_001754"
     CDS             7874..8437
                     /locus_tag="VQ526_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2555452.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8434..9111
                     /locus_tag="VQ526_001755"
     CDS             8434..9111
                     /locus_tag="VQ526_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2555453.1"
                     /translation="MMSNKNKGILIFAILYTILFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
BASE COUNT         2583 a         1793 c         1828 g         2919 t
ORIGIN      
        1 atttcactat agtaaggtgc agcaggataa agaaaaaccg agaagtttct ttctcggatt
       61 tgcttattat gaggttccta ttttctatgc tccaagagtt gattgatgtg atctactttt
      121 ttagcctctc ttttatagag aatagcccag atgatgagat agacaatcgc aaactcgata
      181 atgagctgga ggtagaaaat ccagtggaaa gggaaccaac cagcaagagt tgctagtggg
      241 acaaagccag ccagcataag gaagaaatga gtcagagtgg cacgaagcat gctccagtca
      301 cggctgaata agcggttgcc aaagttgaag agcataccga tggctgccca gataagtgtg
      361 cagtagagca agaccagggc accgtgaacc tgatgctgat ccatcacttg gccgatgaga
      421 gagtagggat ttagtggtgc gtaggtattt ggtgcataaa tgagtgaaaa gaggatggag
      481 aggatgaggc cgataagaac accggcagct gcatcgtgaa atacttgttt tttcatagtt
      541 ctaatttctc cttgatggtt tttagataac ggcgtgaaga gtaggtgaag ctttcgtttt
      601 tcaagaaaat ttctaccaga ccgtttggcg tgagcttgag gtgagagatg gaatcgatat
      661 tgatgatttc tgattgggaa atttggataa aattggttgg caagagttta agaacctgat
      721 agagtcgcaa atcaatgctg taggtctgac tcgcggtttc tgctagaacc ttccgattct
      781 cgatatagaa gcgctgaatc ttgccaatct caactagata gacctgatca tcgattttcc
      841 ctttgatttt ttctctttgg tccagatttt ctgcgaactc gatgactttc tggactttat
      901 ctgtcggctg aggtgcttgg acaatcagct tttcctcctc gtaagtctca ctaatctgta
      961 gttctacttt catagttttc tctccttttc agttatacaa ggttgtgatc acttcctgta
     1021 tatccttatt aaacactatt ttatagccca tggcaaggaa ccttgtctat gtggagggat
     1081 tgggctccta tgtggtggag cttttctgtt ctttctgaaa tatggtataa tagcactaat
     1141 taatttctag gaaaatagat acagaaaggg gctgaaagat gtctcatatt attgaattgc
     1201 cagagatgct ggcaaaccaa atcgcagctg gggaggtcat tgaacgtcct gccagtgtgg
     1261 ttaaagagtt ggtagaaaat gccattgacg cgggctctag tcagattatc attgagattg
     1321 aggaagctgg tctcaagaag gttcaaatca cggataacgg tcatggaatt gcccacgatg
     1381 aggtggagtt ggccctgcgt cgccatgcga ccagtaagat aaaaaatcaa gcagatctct
     1441 ttcggattcg gacgcttggt tttcgtggtg aagccttgcc ttctattgcg tctgttagtg
     1501 tcttgactct gttaacggcg gtggatggtg ctagtcatgg aaccaagtta gtcgcgcgtg
     1561 ggggtgaagt tgaggaagtc atcccagcga ctagtcctgt gggaaccaag gtttgtgtgg
     1621 aggatctctt tttcaacacg cctgcccgtc tcaagtatat gaagagccag caagcggagt
     1681 tgtctcatat cattgatatt gtcaaccgtc tgggcttggc ccatcctgag atttctttta
     1741 gcttgattag tgatggcaag gaaatgacgc ggacagcagg gactggtcaa ttgcgccaag
     1801 caatcgcagg gatttacggt ttggtcagtg ccaagaagat gattgaaatt gagaactctg
     1861 acctagattt cgaaatttca ggttttgtgt ccttgcctga gttgactcgg gctaaccgca
     1921 attatatcag cctcttcatc aatggtcgtt atatcaagaa cttcctgctc aatcgtgcta
     1981 ttttggatgg tttcggaagc aagcttatgg ttggacgttt tccactggct gtcattcaca
     2041 tccatatcga cccttatcta gcggatgtca atgtgcatcc aactaagcaa gaggtgcgga
     2101 tttccaagga aaaagaactg atgaccctgg tttcagaagc tattgcaaat agtctcaagg
     2161 aacaaacctt gattccagat gccttggaaa atcttgccaa atcgaccgtg cgcaatcgtg
     2221 agaaggtgga tcaaactatt ctcccactca aagaaaatac gctctactat gagaaaactg
     2281 agccgtcaag acctagtcaa actgaagtag ctgattatca ggtagaattg actgatgaag
     2341 ggcaggattt gaccctgttt gccaaggaaa ccttggaccg attgaccaag ccagcaaaac
     2401 tgcattttgc agagagaaag cctgctaact acgaccagct agaccatcca gagttagatc
     2461 ttgctagcat cgataaggct tatgacaaac tggagcgaga agaagcatcc agcttcccag
     2521 agttggagtt tttcggacaa atgcacggga cttatctctt tgcccaaggg cgagatggac
     2581 tttacatcat agatcagcac gctgctcagg aacgggtcaa gtacgaggag taccgtgaaa
     2641 gcattggcaa tgttgaccag agccagcagc aactcctagt gccctatatc tttgaatttc
     2701 ctgcggatga tgccctgcgt ctcaaggaaa gaatgcctct cttagaggaa gtgggcgtct
     2761 ttctagcaga gtacggagaa aatcaattta ttctacgtga acatcctatt tggatggcag
     2821 aagaagagat tgaatcaggc atctatgaga tgtgcgacat gctccttttg accaaggaag
     2881 tttctatcaa gaaataccga gcagagttgg ctatcatgat gtcttgcaag cgatctatca
     2941 aggccaatca tcgtattgat gatcattcag ctagacaact cctctatcag ctttctcaat
     3001 gtgacaatcc ctataactgt cctcacggac gtcctgtttt ggtgcatttt accaagtcgg
     3061 atatggaaaa gatgttccga cgtattcagg aaaatcacac cagtctccgt gagttgggga
     3121 aatattaaaa gtataaaaaa gtctgggaaa aattttcaaa atcagaaaaa cgcataaaat
     3181 caggtgttca aaaaccttga ttttatgcgt tttatcatgg aaatagttac ttcatgtttt
     3241 cctaattctt ttcgaaactc tttttaaacc acgtcagttt tatcagtaat ctcaaaacag
     3301 tgttttgagc taattttgcc agttttgtct gtaacatcga agttgtgttt gaccactctg
     3361 cgactggttt cctagtttgc tctatgattt tcacagagta ttaaattgcg attttgccaa
     3421 gtttctttat tcgtctaaaa gtagagtctg ttctatgcgt ctaatgtacg aattaggttg
     3481 accatttcaa tagctccttg tgcacactca gaacccttat ttcctgcttt agtgccagct
     3541 cgttctatgg cttgttcaat tgtatctgtc gttagcacac caaacataac aggaatttcg
     3601 ctatttaaac tgatttgggc gattccctta gatacctcgc tacatacata atcataatga
     3661 cttgtattcc ctctaatgac agctcccaag cagataattg catcatattt tttacttttt
     3721 gccatttttg atgcaatcag tggtatttca aaagctcctg gaacccaggc tacctctata
     3781 tctttctcgt ttacattctc tcttttgaga ttatctagtg ctccagataa taattttgaa
     3841 gttataaatt cattaaatct cgctacaaca atacctattt taatattgtt tgctactaaa
     3901 ttaccttcat aagtgttcat ttatttttcc tccatattta aaatgtgacc cattcgattt
     3961 ttctttgttt ctaaataaaa actatcgtaa ggattggctt ctatttcgat tgatattcta
     4021 ctggaaatgg taattccata tttttctaac tgttcaacct tgtcaggatt atttgtcagt
     4081 aaatgaagtg actgaagtcc cagatcttta agcatttgtg ctccaatatg atattctctt
     4141 aaatcacctt caaagcctaa tgcaagattg gcatcaagcg tatccatgcc ttgatcttgt
     4201 aaatgatagg cttttaattt attgataagt ccaattcctc gtccctcctg tcgcaagtaa
     4261 agtaagacac ccgaaccatt ctcaacaatc attttcatag ctttatcgaa ttgctgtcca
     4321 caatcgcaac gtaaagagcc taaaacatct cctgttaaac attcggagtg gacccgacat
     4381 aatacattgg cttcatcctc tatgtttccc ataataagag caagatgatg ttccccattt
     4441 agtttatcta tatagctaat tgctttgaaa ttaccgtatc tagtaggcat attgacagtt
     4501 gaaactcgtt ctaccagctg atcatatact tttctatatt cttgtaattc tttgatggta
     4561 attagtggaa tgttgtgttt cttcgagaac tgaattaaat catctgttct catcattttg
     4621 ccatcatgat tcattatttc acaacatagg ccacactctt ttagtccagc taattttaat
     4681 aaatcaacag ttgcttctgt gtgtccattt ctttctagaa caccaccttt ttttgcaatt
     4741 aaaggaaaca tgtgtcctgg cctgcgaaaa tcagagggtg ttatatcttc agctacacac
     4801 atacgtgcgg tcagtcctct ttcctcggca gaaatacctg tggtcgtttc tttataatca
     4861 attgaaactg taaaagcagt cttatgatta tctgtattgt tttcaaccat aggtgaaagc
     4921 attaattgat tagctaaact ttcgctcata ggcatacaaa ttaatccttt ggcataagta
     4981 gccataaaat taacattttc tgttgtagct gcttgtgcag aacaaattaa gtctccttca
     5041 ttttctctat ccttgtcgtc tataacaaga acaagtcgtc ccttctgcaa tgcttctaat
     5101 gcttcttgta tttttcgata ttccattgac tgattatcct ttctgctaaa atccattttg
     5161 atataatagt tccttagata tttctgattt tggagagtta tccatcagtt tttgcacata
     5221 tttacctaag atatcatttt caagatttac tgtactcccg acttgtttac tcttaagaat
     5281 ggtttgttcc aaggtatgag ggataacaga tactgaaaag tttactttgg agactttagc
     5341 gacagtcaga ctaatgccgt caattgtaat agatcctttt tcaactatta aatctaaaat
     5401 ttctttttgt gtgttgattt gataccatac agcattatca tcttttttta ttgacgagat
     5461 ttttcctgta ccatcaatgt gtcctgtaac gacgtgaccc ccaagtcgac cgttgacaga
     5521 taaggctctt tctagattca cctcacttcc atgttttaat agagtaagag ctgttcgact
     5581 ccatgtttca ttcattacat caactgtaaa ggattgatga ttgaaatgag taactgtaag
     5641 acagatacca tttactgcta tactatcgcc taaatggata tccgttaata tttttgaggc
     5701 tttaattgat agtttacaat tacgagagtc tttctgtatt ctttcaactt ttccgatttc
     5761 ttcaattatt cctgtgaaca tggataaatc acttcacttt ctatgagata gtcatttcct
     5821 atttgagaaa atgcataagg tttcaatcta atagcgtcat ttggcaaaga aatgccttca
     5881 cctccgacag gaaacttggc actgcctcca aaaatttttg gtgcaatata tattttcagc
     5941 tcatcaacaa tttgttgttc caaagcactc caattcatta gactgccccc ttctagaact
     6001 aggctatcaa tctgcatgtt tcctagatgt tgcattaaac tcgataagtc tatatgattg
     6061 ccttttttct ttatggaaag tatttcacag ccatgatttt gatatagctt cattttattt
     6121 ttgtcttcag aggaagtggc aatgtaagtt ttaatatcat ttgctgtttt tacgatttta
     6181 gaggtaagag gagttcgtaa atgtgtatcg catatgatac ggataggatt tttcccttcc
     6241 tccaatctac atgtcagcaa aggatcgtct tgaataacag tattgactcc caccataatt
     6301 gcactaacat ggtgtcgtaa ctgatgcaca tgctttcttg cttcttcttc agtaatccat
     6361 ttggattgat ttgttttagt ggctattttt ccatccattg acattgcata tttcataaaa
     6421 acatagggta catgctgggt aatatacttt ctaaaacttt ttattaagtt aagacactca
     6481 ttttctaaaa ttccaacagt aacttgaaga ttattttcct caagtatctt tactcctttt
     6541 ccagatacaa taggattaca gtctaggctt ccaatgacta ctcttgtaat accactatcg
     6601 attatagcat ctatacaggg aggtgttttc ccgaagtgac aacagggttc aagtgttaca
     6661 taaagcgtcg ctccgacagg ggattttcta cagtttttaa gagcatttct ctcagcatgt
     6721 gggccaccaa aaaactcatg ataaccttgt ccgataatgt gattatcttt tacaataatt
     6781 gcgccaacca taggattggg attgacgtaa ccagcccctt tttgtgccag ttttattgct
     6841 aatttcatat attttgaatc gctcatctca ctacctccaa aaaaatatac cttgaatagg
     6901 ggactactca aggcatacaa aagaaaactt atgcgattaa caaaaatgct ctgaaatgac
     6961 aagtaatcat ttcagagcac gcaaaaagca caaatatact tttatcttct ttcatccaga
     7021 ctatactgtc ggctttggaa tttcaccaaa tcatgccttt cggctcgtgg gctataccac
     7081 cggtagggaa ttgcaccctg ccctgaagat agttattcaa ttacagatga ttatagtact
     7141 taattttgaa tatgtcaaca gataaataca gattgttttt gatatactgt atttgtgata
     7201 atcgattctc gctcctcgga taaagaaaat atgatatact agataaacga aataagagag
     7261 aaggaacact atgtacgcat atttaaaagg aatcattacc aaaattactg ccaaatacat
     7321 tgttcttgaa accaatggta ttggttatat cctgcatgtg gccaatcctt atgcctattc
     7381 aggtcaggtt aatcaggagg atcagattta tgtgcatcag gttgtgcgtg aggacgccca
     7441 tttgctttat ggatttcgct cagaggatga gaaaaagctc tttcttagtc tgatttcggt
     7501 ctctgggatt ggtcctgtat cagctcttgc tattatcgct gctgatgaca atgctggctt
     7561 ggttcaagcc attgaaacca agaacatcac ctacttgacc aagttcccta aaattggcaa
     7621 gaaaacagcc cagcagatgg tgctggactt ggaaggcaag gtagtagttg caggagatga
     7681 ccttcctgcc aaggtcgcag tacaagcaag tgctgaaaac caagaattgg aagaagctat
     7741 ggaagccatg ttggctctgg gctacaaggc aacagagctc aagaaaatca agaaattctt
     7801 tgaaggaacg acagatacag ctgagaacta tatcaagtcg gcccttaaaa tgttggtcaa
     7861 ataggagcag agaatgacaa aacgttgttc gtgggtcaag atgaccaacc cgctctacat
     7921 cgcctatcat gatgaggagt ggggtcagcc cctccatgat gaccaagcat tgtttgagtt
     7981 gttgtgtatg gaaacctatc aggcaggcct gtcttgggaa acggtactca acaaacgcca
     8041 agctttccga gaagcctttc atagctatca aattcactca gtcgcagaga tgactgacac
     8101 tgaattggaa gccatgctgg agaatccagc tatcattcga aatagagcca agatttttgc
     8161 tacacgcgct aacgcccaag cctttctaca gttacaggca gagtacggct cttttgatgc
     8221 ctatctttgg tcttttgttg aggggaaaac tgtcgttaac gatgttcctg attatcgcca
     8281 agccccagct aaaacaccct tatctgagaa attagccaaa gatctcaaaa aacgaggctt
     8341 caagttcaca ggcccagtcg ccgtattgtc ttttctacag gctgcagggc tagttgatga
     8401 ccacgagaat gattgtgagt ggaaaggtct taaatgatgt ctaacaaaaa taagggaatt
     8461 ctgatttttg cgattctcta tacaatcctc tttgtgtttg atggcgttaa attgctggct
     8521 tctttaatgc catctgtcat tgcaaattat cttgtttatg tagttttagc tttatatggc
     8581 tccttcttgt tcaaggatag attgatccaa caatggaagg agattagaaa gactaaaaga
     8641 aaattcttct ttggcgtctt aacaggatgg ctctttctca ttctgatgac tgttgtcttt
     8701 gaatttgtat cagagatgtt gaagcagttt gtgggattag atggacaagg tctaaatcag
     8761 tctaatattc aaagtacctt tcaagaacaa ccactactga tagctgtttt tgcttgtgtc
     8821 attggacctc tggtagaaga attatttttc cgtcaggtct tattgcatta cttgcaggaa
     8881 cggttgccag gtttactaag cattattctg gtaggacttg tttttgctct gactcatatg
     8941 cacagtttgg ctctatcaga gtggattggt gcagttggtt acttaggtgg aggccttgcc
     9001 ttttctatta tttatgtgaa agaaaaagag aatatctact atcccctact tgttcacatg
     9061 ttaagcaaca gcctctcctt aatcatttta gctatcagta tagtaaaatg aaatgagaac
     9121 agg
//
LOCUS       DAVBDG010000070         9111 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBDG010000070 DAVBDG010000000
VERSION     DAVBDG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.071"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(161..631)
                     /locus_tag="VQ526_001756"
     CDS             complement(161..631)
                     /locus_tag="VQ526_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2555454.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(648..2921)
                     /locus_tag="VQ526_001757"
     CDS             complement(648..2921)
                     /locus_tag="VQ526_001757"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2555455.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGQAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3267..6368
                     /locus_tag="VQ526_001758"
     CDS             3267..6368
                     /locus_tag="VQ526_001758"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2555456.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLSLSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            6451..7458
                     /gene="pfkA"
                     /locus_tag="VQ526_001759"
     CDS             6451..7458
                     /gene="pfkA"
                     /locus_tag="VQ526_001759"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2555457.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            7517..9022
                     /gene="pyk"
                     /locus_tag="VQ526_001760"
     CDS             7517..9022
                     /gene="pyk"
                     /locus_tag="VQ526_001760"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2555458.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHIQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
BASE COUNT         2785 a         1585 c         2036 g         2705 t
ORIGIN      
        1 gttgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg
       61 catagtatca agtgttgaaa acccttgata ctatgcgttt tctgatgtta aagactttct
      121 accagttttt ttaagcataa ttgttagttg tagtcattta ttattcttca aagaaaaatg
      181 gtggggcgaa ttttttcagt tcttcaaagc acttttgagc agtatctgca tcttcacaga
      241 tgataagaca gacatcatta ccacaaaggg tagcgatagc gtcagggaag ctcaaagtat
      301 caatgataga accaaaggat tgagccagtc caggaagggt ttttagtagg acttggtgtt
      361 gaactgggcg catccagaca agggcgtctt ccatgtagag ttcgagacgt ttttcccatt
      421 ttgagatgga accattgtta agaacataat aagcgctatc ttcttcgcgg acttttgata
      481 ggttcatatt tttgatgtcg cgtgagaggg ttgcctgggt tacttgaatg tcgttcttag
      541 caagaagggc ttgcaactca gcctgtgtat gaatcttgtt ttttgtgata agagcgcgta
      601 taagttggtg gcggtgttct gatttattca taataatgta actcctttta gcaaggtaag
      661 gtaagcatgg actgagcgag gtcgacagtc aagtggtagt ctgtattgtc acggatggtg
      721 atttcaaagt cagtagtata gaggactaaa cggagagtgt ctccttcttt tagcttgtaa
      781 atagttggct gcagttcaaa ttgaacgtcc atccattcat ctgcagtaat atcctctact
      841 aacagtaaat catttctatt ttgtaaatta aggtaacctt ttgtcacgac tcgttgtgcc
      901 tctggtctaa atggcaattc acagagattt tccaacatgt gatagcgacc gttatcaatg
      961 gttctagcac ttaaaatagc tggataaggt tgtaggtatt tcttttgccc aaattctagc
     1021 agttgggcag ataagagccc cttgtttgta ctggatttga tacgaagatt gagctgagcg
     1081 cgaccgttta ggtggagatc tttagtcaca ggaaggttaa tagtaatctg attagctttc
     1141 ccttgataga gctctgtatt gaaggtttgg tatgtcttac tatagcgctc aaaatcctta
     1201 tctgggtact ggttttgaat agcttgctct tcttgaccaa gtgagaaggt tttacagttt
     1261 tcttgcccac cgaagttatc aagtgataac caagtctgtg gagctgtatt gtcctgccag
     1321 ataacagtag gaagttgaaa gtctgtttcc tgtcctagta atttcttggt taataaggca
     1381 tttatggact cacggaagtc aattgattgc caattgttca tgtaaacatg ggcaccatta
     1441 tggaaaaaga gatgcttgtg tatatgagta ggaagagcat ggaacatctg gtaaacatga
     1501 agtggtttga cattccaatc ctgagaacca tgagtaaaga caacctctgc ctttacttta
     1561 tgggcattga gcagataatt gcggtcatgc caaaactgat tgtagtcccc agtttttcgg
     1621 tctagctgag ctttcacttt ttctaagtca gcttggtgag cttcattgcc acggatatag
     1681 tcgccagcta agagattacg agaataggtt aactcagcaa gggagtcaaa gtcctcacct
     1741 ggataaccac ctgggctagt caccagaccg ttttcacggt agtagttgta ccatgatgaa
     1801 attcctgcct cggcaatgat aacttctaaa ccatcgactc ctgtagtcgc aagaccattg
     1861 gacatggtac ctagatagga aagtcccgtt gtagcaactt ttccgtttga ccaatcagcc
     1921 ttgacttgac gctggcgcgt gtgatcagta aaggcacggc aacggccgtt aagccaatcg
     1981 atgacatttt tataagcctc gatttgctgg tagtctccat tagtcatgaa acctgtcgag
     2041 tctttggtac caacacctga gacatagaga ttggcaaagc ctctcggaag gaagtagtcg
     2101 tttagtgtat agctagagtt gatgtgagtt agcttttcct cagcctctgc tataagctca
     2161 gcttgacctt ggggttggac gagatttagt tgaggtttct ctagctcaat cttgtgagga
     2221 agcttaacct caagctcgcc ctccatcttg tagagagcct tgtcactagc cttgtcattg
     2281 gttccctgat gataagggct ggctgtcatg atggcaggga tttttccatc aaaacgaggg
     2341 cgaataatgc taacctttac taggtctgat agcccttttt ggtcagtatc gacacgagac
     2401 tcaacgtaaa cgacttcacg aatgacatcc tggttagaaa aagtagccaa actcttgccg
     2461 ttaaagtagt ggtagtcatt atcctccgga ataagaccat cactaacaag ttggtcgata
     2521 agagtatttc cttttttggt gcgagtattg agtaactgat agagattttc aatcaagtca
     2581 ccatatataa tgggaaatcc agtttcttta cgaaaaacgt cactatcttc gaagtcaacc
     2641 aaataagaaa agcctaaaag ttgaaaagca acagtataaa aaatatctgc tgtcagttca
     2701 tcttctgatt gaaaaaatgt cagcaggtct gtttttttat cagctgctag gatagaaagt
     2761 gggtagttgg tgtcttgata agtgaaaaag aaacgacgta aaaaggtttc aagtgagtct
     2821 ttgtgattgg ctgtattttg taaatcaaag ccacattttt ttagctcaga taagacattt
     2881 tcttttggaa aattgatata actatattga ttaaaacgca tagaacctcc atatagaatg
     2941 acagttaagg ttattatatc aaaaaaaaag cagaaaggga attgttaact tcaaaaggaa
     3001 ataatccaat aaaaatgaat aaagtactaa attcaatata gagaacagag taacaataag
     3061 aataatagat agggtataaa agttctagga gatttatatt atatgctttc tatttttata
     3121 tacaatatag tataaatata aaaatgatga caaaaataca aatgaataga aaataaatta
     3181 gtaagctgat gaaatttttc tcaagagaag ccatttatag gtgaaaatgg tataatatag
     3241 tgagaaggat agaggagaag tgtaaattga tcgcacaact agatacaaaa acagtctata
     3301 gttttatgga aagcgtcatt tcgatcgaaa agtatgtgag agcagctaaa gaatacggct
     3361 acactcattt ggctatgatg gatattgaca atctttatgg cgctttcgac tttctagaga
     3421 ttacaaaaaa atacggcatt catcctttgc tagggcttga aatgacagtg tttgtagatg
     3481 atcaggaagt gaatttgcgc tttttatctc tatctagtgt gggctatcag cagttgatga
     3541 agctttcgac agccaagatg cagggggaga aaacttggtc agtcctgtcc cagtacctgg
     3601 aggatatcgc ggtcattgtg ccttattttg atagagttga gtcgttagaa ctaggctgtg
     3661 attactatat aggggtttat ccagaaacac tagcaagcga atttcatcat cctatcttac
     3721 ctctttatcg ggtcaacgct tttgaaagca gggatagaga agttcttcaa gttttaacag
     3781 cgattaaaga aaatctaccg ctcagagaag ttcccttgcg ttcgagacaa gatgtcttta
     3841 tatcagcaag ttctttagag aaactattcc aagagtgttt tccgcaagct ttggacaatt
     3901 tagaaaagct tatttcaggc atttcttacg acttggatac tagtctgaaa ctgcctcgtt
     3961 ttaatccagc tagaccagca gtagaggagt tgagagagcg tgctgaactg gggcttgttc
     4021 agaaggggtt gactagtaaa gaatatcaag atagactaga ccaagaattg tctgttattc
     4081 atgatatggg ctttgatgat tatttcttgg ttgtttggga tttgttgcgt tttggacgat
     4141 cgaatggcta ttatatggga atgggaaggg gttctgcagt aggcagtttg gtttcttatg
     4201 ccttagacat cacggggatt gacccagtag agaaaaatct gatttttgaa cgctttctta
     4261 atcgtgaacg ctataccatg cctgatattg atattgatat cccagatatt tatcgtccag
     4321 attttatcag atatgttggt aataaatatg gtagtaaaca tgcggcacaa atcgttactt
     4381 tttcaacctt tggagccaag caagctcttc gagatgtctt gaaacgcttt ggtgtgccag
     4441 agtatgaatt atctgcaatt actaagaaaa tcagttttcg tgacaatctt aagtcggcct
     4501 atgagggcaa tctccagttt cgtcagcaaa tcaatagtaa gttagaatac caaaaagctt
     4561 ttgagattgc ttgcaagata gagggctatc caaggcaaac ctctgtccat gcggctggtg
     4621 ttgtaattag tgaccaagat ttaaccaact acattcctct aaagtatggt gatgaaattc
     4681 cactgactca gtatgatgct catggagttg aggctagcgg acttttgaag atggactttc
     4741 tgggactacg aaatttgacc tttgtccaga agatgcaaga gttgcttgct gaaacagaag
     4801 gtattcacct taaaattgaa gaaatagatt tggaagacaa agaaacgtta gatttatttg
     4861 cctctggaaa tacaaaaggt atctttcaat ttgagcaacc tggtgccatt cgcttgctca
     4921 aacgtgttca accagtctgt tttgaagatg tcgtagcaac tacttctcta aatcgaccag
     4981 gtgcaagtga ctacatcaat aattttgtgg caaggaagca tgggcaggaa gaagtgactg
     5041 ttctagattc agctctggag gatattttgg ctccaactta tggcattatg ctctatcagg
     5101 agcaggttat gcaggttgct cagcgatttg ctggatttag tcttgggaag gccgatattt
     5161 tgcgtcgagc tatgggtaaa aaggatgcct ctgctatgca tgagatgagg gcttccttta
     5221 ttcaaggctc catagaagca ggccatactg cggaaaaagc agagcaggtt tttgatgtta
     5281 tggagaagtt tgcaggttat ggatttaata gatctcatgc ctacgcctac tcagccttgg
     5341 ccttccagtt ggcttatttc aaaacgcatt atccagccat tttttatcag gtcatgttaa
     5401 attattccaa cagtgattac ttaatagatg cacttgaagc aggttttgaa gtagcctctc
     5461 tatccatcaa tactattccc tatcacgata aaattgccaa caagtctatc tatataggtt
     5521 tgaaatcgat taaggggctc agcaaggact tggcgctttg gattattgaa catagacctt
     5581 attctaacat tgaagatttt atagctaaat tacctgagaa ttatctgaaa cttcctctgc
     5641 tagaaccttt ggtaaaagtt ggtcttttcg attcatttga aaaaaatcgt caaaaagtat
     5701 ttaataactt agctaatcta tttgaatttg tgaaagagtt gggaagtttg tttggagatg
     5761 ctatttatag ttggcaggaa tcggaagatt ggacggaaca agaaaaattt tatatggaac
     5821 aagagctttt agggataggt gtcagcaaac atccactaca agctattgca agtaaggcta
     5881 tttacccgat taccccaatc ggaaatttgt cagaaaatag ctatgctatt attttggttg
     5941 aagttcagaa aataaaagtg attcgtacca aaaagggtga aaatatggcc ttcttacagg
     6001 cagatgatag taagaaaaaa ttggatgtca ctctcttttc agacttatat cgtcaggttg
     6061 gacaggaaat aaaagaggga gccttctact atgtaaaagg aaaaatacaa tcacgtgatg
     6121 gccgtttgca aatgattgca caagaaataa gagaagcagt tgctgaacgc ttttggatac
     6181 aggtgaaaaa tcatgaatcg gatcaagaaa tttcacgtat tttagaacaa tttaaaggcc
     6241 caatcccagt catcatccgg tatgaagagg aacagaaaac catcgtttct ccccatcatt
     6301 ttgtagctaa atccaatgaa ttagaggaga aattgaatga aatcgttatg aaaacgattt
     6361 atcgctaaaa atacggaaaa tagaagaatt ttaaaatcaa atgtgatata atcaagaaga
     6421 atgttaaaag aaaaaggagc ataaaccaat atgaaacgta ttgctgtttt gactagtggc
     6481 ggagacgcac ctggtatgaa cgctgctatc cgtgcagttg ttcgtcaagc aatttcagaa
     6541 ggaatggaag tgtttggtat ctatgacgga tatgctggta tggttgccgg tgaaattcat
     6601 cccctagatg cagcttcagt aggagacatc atttctcgtg gtggtacttt tcttcactca
     6661 gctcgttacc cagagttcgc tcaacttgaa gggcaactta aagggattga gcaattgaaa
     6721 aaacacggaa ttgaaggtgt agttgttatc ggtggtgacg gatcttacca cggcgctatg
     6781 cgtttgactg aacatggctt cccagctatt ggtcttccag gtacaatcga taacgatatc
     6841 gttggtactg actttacaat cggttttgac acagcggtta ctactgccat ggacgctatc
     6901 gataagattc gtgatacatc atcaagtcac cgtcgtactt ttgtaatcga agttatggga
     6961 cgtaacgctg gtgatatcgc tctttgggct ggtattgcaa ctggtgctga tgaaatcatc
     7021 atccctgaag caggcttcaa gatggaagat atcgtagcaa gcatcaaagc tggttatgaa
     7081 tgtggtaaaa aacacaatat catcgtctta gctgaaggtg tgatgtcagc ggctgaattt
     7141 ggtcaaaaac ttaaagaagc tggagataca agcgaccttc gtgtaacaga acttggacat
     7201 attcaacgtg gtggttctcc aactgcgcgt gaccgtgttt tggcgtcacg tatgggtgca
     7261 catgctgtta aacttcttaa agaaggtatc ggtggtgttg cggttggtat tcgtaacgaa
     7321 aaaatggttg aaaatccaat tcttggtact gcagaagaag gggcattgtt tagccttact
     7381 gcagaaggta agattgtggt taacaacccg cacaaagccg atattgagct atctagcttg
     7441 aataagagct tgtcataatt tacaatttag ttaataagac caaaaaggtc atatatataa
     7501 aggagtcaca aaaatcatga acaaacgtgt aaaaatcgtt gcaactttgg gtcctgcggt
     7561 agaaatccgt ggtggtaaaa aattcggtga ggacggatac tggggtgaaa aacttgatgt
     7621 tgaagcttca gctaaaaaca ttgctaaatt gattgaagct ggtgctaaca cattccgatt
     7681 caacttctca cacggcgacc accaagaaca aggtgagcgt atggcaactg ttaaacttgc
     7741 ggaaaaaatt gcaggtaaaa aagttggttt ccttcttgat acaaaaggac ctgaaatccg
     7801 tacagaattg ttcgaaggtg aagctaaaga atattcatac aaaactggtg aaaaaattcg
     7861 tgttgcaact aaacaaggaa tcaaatcaac tcgtgaagtg attgcgttga acgttgctgg
     7921 tgctcttgat atctatgatg atgttgaagt tggtcgtcaa gttttggttg acgatggtaa
     7981 acttggtctt cgtgtggttg ctaaagatga tgcaactcgt gaatttgaag ttgaagttga
     8041 aaacgatggt atcatcgcta aacaaaaagg tgtgaacatc cctaacacta aaattccttt
     8101 cccagctctt gctgaacgcg ataacgacga tatccgtttc ggtcttgaac aaggtatcaa
     8161 cttcatcgca atttcattcg tacgtactgc aaaagatgtg aacgaagttc gtgcaatctg
     8221 tgaagaaact ggaaacggac atattcaatt gttcgctaaa atcgaaaacc aacaaggtat
     8281 cgataactta gacgaaatca tcgaagcagc tgatggtatt atgattgctc gtggtgatat
     8341 gggtatcgaa gtaccgttcg aaatggttcc agtttatcaa aaaatgatta tcaagaaagt
     8401 caatgctgca ggtaaagttg ttatcactgc aacaaacatg cttgaaacaa tgactgaaaa
     8461 accacgtgca actcgttcag aagtatcaga cgtattcaac gctgttatcg acggaactga
     8521 cgctacaatg ctgtcaggcg agtctgcaaa cggtaaatac ccactcgagt cagtaactac
     8581 aatggctaca atcgacaaga acgctcaagc tcttcttaat gaatacggac gtcttgattc
     8641 agattcattt gagcgtaact ctaagacaga agtaatggct tctgctgtta aagatgctac
     8701 taactcaatg gatatcaaat tggttgtaac tcttactaag acaggtcata ctgcacgttt
     8761 gatttctaaa taccgtccaa atgctgacat cttagcattg acatttgacg aattgacaga
     8821 acgtggcttg atgttgaact ggggtgttat cccaatgttg acagatgctc catcttcaac
     8881 tgacgatatg ttcgaaatcg ctgaacgtaa agcggtagaa gcaggtctcg ttgagtcagg
     8941 cgatgatatc gttatcgttg ctggtgtgcc agtaggagaa gctgttcgca caaacacaat
     9001 gcgtatccgc acagtacgtt aagaaaaata taaaaaccta tcatatccag ctttctagcc
     9061 tgtatgatgg gctctttttt tgtccagggg gcaaaacttt cataatggat t
//
LOCUS       DAVBDG010000071         9016 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBDG010000071 DAVBDG010000000
VERSION     DAVBDG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.072"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(357..1964)
                     /locus_tag="VQ526_001761"
     CDS             complement(357..1964)
                     /locus_tag="VQ526_001761"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2555459.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2354..2941)
                     /locus_tag="VQ526_001762"
     CDS             complement(2354..2941)
                     /locus_tag="VQ526_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2555460.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(3069..3518)
                     /locus_tag="VQ526_001763"
     CDS             complement(3069..3518)
                     /locus_tag="VQ526_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555461.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3523..3912)
                     /locus_tag="VQ526_001764"
     CDS             complement(3523..3912)
                     /locus_tag="VQ526_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HET2555462.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3940..4566)
                     /locus_tag="VQ526_001765"
     CDS             complement(3940..4566)
                     /locus_tag="VQ526_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2555463.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4580..5143)
                     /locus_tag="VQ526_001766"
     CDS             complement(4580..5143)
                     /locus_tag="VQ526_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2555464.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5308..5542)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5596..6099)
                     /locus_tag="VQ526_001767"
     CDS             complement(5596..6099)
                     /locus_tag="VQ526_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2555465.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6292..7122)
                     /locus_tag="VQ526_001768"
     CDS             complement(6292..7122)
                     /locus_tag="VQ526_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2555466.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(7107..8789)
                     /locus_tag="VQ526_001769"
     CDS             complement(7107..8789)
                     /locus_tag="VQ526_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555467.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETSELQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
BASE COUNT         2724 a         2063 c         1644 g         2585 t
ORIGIN      
        1 ggattaaatt aaaaaagtat aatgtattga atctataaca gtacatcttg actgctaaaa
       61 tatttctata aattaatttg acttttctga tagagatgtt cacatcttat ttcaattcac
      121 tatacaatcc agttcctaaa actttgctag aaattgattt ggctttccga attaatttgt
      181 tcaaatttta tttcatttca atatactgat aaatccattc actagcacag ggcaagaggg
      241 agtcgctatt gatttcaacg gtagcaggta catacagagg actcctacct cagatatact
      301 gcaatatcat cgtatgcaaa aaacctctga gatttttctc aaaggttctg attttgctaa
      361 ttgctgttct caaccgctgc agtaacaaag gcagtgtaga gttcttctgg tcggtttgga
      421 cggcttgaca gttcagggtg atactgacaa gctacaaaga atttattttc aggaatttcc
      481 acgatttcta ccaaacgatt gtctggagaa actcctgaaa agacaaaacc tgctgcctca
      541 aactgctcac ggaaggcatt attaaactca taacggtgac ggtgacggcg ttgcaccact
      601 tcttgattgt gataagcagc agcagcctta gagccacgtt tcaacttaga cggataaagt
      661 cccaaacgaa gggttccacc catatcctca atatcaatct gatcacgcat gatatcaatg
      721 atagggtatt ttgtttctgg tgcaagctct gcagaattgg caccttcaag acctaaaacg
      781 tgacgagcaa actcgataca tgtcaactgc attcccaagc agactcccaa cattggaaca
      841 tcattttcac gcgcatagcg gatggcttgg attttccctt ctgtaccacg ttgaccaaaa
      901 ccacctggta cgatgatccc gtccgcatca gacaagagtt ctgctacatt ctctgctgtc
      961 acatcattgg cattgaccca attgatttta acttctacat cattgacata gccagagtgt
     1021 ttcaaggctt cgaccactga gatataggca tcttgcaact ccacatactt accaacaagg
     1081 gaaatcttaa cttgtttctt gaggttcatg accttgtcca ccatggctga ccattctgtc
     1141 atatccgctg ctggtgcgtc taatttcaaa tgatcacaaa caatttggtc catcccttgt
     1201 gcctgcaagt tcagtggaat ttggtaaagg tgttcaacat ccaacgattc gataacggct
     1261 tctggtgcca catcacagaa ctgggccagt ttatttttaa ttccttgacc agctggctct
     1321 tctgtacgaa taaccaacat atttggttgg attcccaagc cacgcaattc tttgacagag
     1381 tgttgggttg gtttggtttt catttcacca gcagccttga ggtaaggaag caaggttgta
     1441 tggatataca tgacattatc cgcacccaca tctgccttca tctgacgaag agcctctagg
     1501 aatggcaagg actcgatatc tcctactgtt ccaccaacct ctgtgataat gacatcagag
     1561 tcggtcgtta gagcggcacg cttgattttt tctttcaaag catctgtgat atgaggaatg
     1621 acttgaacag ttgccccaag gtattctcca cggcgttctt tacgaagaac ttcactgtaa
     1681 attttcccag ttgtcacgtt ggaatatttg ttgagattga tatcgatgaa acgttcatag
     1741 tgacccaagt ccaaatctgt ctcagctccg tcatctgtca caaaaacttc cccgtgctgg
     1801 taaggactca tggttcccgg atcaatattg atataagggt caaacttttg aatggttact
     1861 ttgagaccac gatttttcaa gagacggcct agactcgctg ccacaatccc tttcccaatg
     1921 gacgatacca caccaccagt tacaaaaata tatttcgtag acatagattc ctctttctaa
     1981 aatgctcaag gtcttgttaa ctacgagggg atatagaaaa caagacccta ctaataagaa
     2041 taatctggga cgactgcacc agattcacaa ctaatactct tcgaaaatct cttcaaaccg
     2101 cgtcaacgtc gccttgccgt agatatgtgt tcctgacttt gtcagtctta tctacaacct
     2161 caaaacagtg ttttgagcag cctacggcta gtttcctagc ttgctctttg attttcattg
     2221 agtataaaat acaagaagat aacttcttga aaaaacaaaa atagctccct aagaactagg
     2281 gagccccgac ctctaaaaga ggtgcccaaa caatatgata cctaaaaata ggataattgt
     2341 caatagctaa ctcttattcc tcgctggttt cagcgtcgtc atctgaaaac tcgtcgtcgt
     2401 cttcgttgag atccacatct tctcccaagt catctggagc gatttcgttg atttctgcat
     2461 cataagcttc cacttcattt ttttcatcat ctggattttc atcatcgtat gaaagagctg
     2521 gatctgcttc gtatgcatct tcgtcttctg gatcatctgc attgtagtca atggcatctg
     2581 aatcaccatc cataaaggca ttgacacgtt ttttcttagc ttttggtgct acttcatcgt
     2641 cgtcattttc ttcaagagcg atgatttctt cgtcgatttc gtccacacca taccatgaac
     2701 gaagacccca tttgttgtcc ccaagtgaga tgaagctacc gtcaaagttc aactctgtgt
     2761 agaacaaagg caaagcttcg cggatatcgc tgtttgatgt tccaaggtag ttttgaattt
     2821 cgtttacaag atcgctaaaa tgcatctcat gatcgcgacc acgaagttcc aagatagcac
     2881 gcgctacctc aatcatagat agttcacttt tttcttgccc agcaaatact tctaattcca
     2941 aagcgtttct cctcatttat actactatcg ccagagcgaa cagactctga cctcatttta
     3001 tcatttactc tttattttac gataattttg cggaatagtc aaaggttaag ggggagaaag
     3061 tggcaggatt agactaattc caatataaaa ctccttcctt tttctgttgc tccattttcc
     3121 acaaatccaa gcgacttgaa acacctccta gaagcatgat tgtaggtgta gatttccttg
     3181 actctcaatt ctttccatcc ttttactcga gccaattcaa tcaaagcact tagaatcttt
     3241 tttccaagtc ctcgatgttg gtaagcggaa ttcccaatca caatggggag attatcctga
     3301 gatagtgtaa tatccccaat tggaaaccat tctcccttct ccttgacttc aatccaaaaa
     3361 agctcaccat gccgatccaa ataggaatac atggcttcca aggtcgcttg actgtaagga
     3421 agcttcaccc catctacgag gtaaaccaag ttcacatcct gataccaagc aaaagcttcc
     3481 tcacaataat tgaccttata ataaggaaca aaacacaatg cactaccttt tcaacaaaag
     3541 agttgctgct tgattaaaac catcacacca gttataccat tttgcttcat acccatcttg
     3601 agctaggata cgatcttcta aatcaaaaac agagtaaatc tttctttcct cgcaagcttg
     3661 cgcatagaga tgatatagtt catcaccacc atctctatcc cactcagcag aaatcgtatc
     3721 ccgacctgcc aataaagcct gataagccct gtgatgccca tctgtaatca gcaaacaatc
     3781 tccaaaggca agaatactga ttggatcgac ttggattgtt tctgccgact ggtaaagcat
     3841 ctgaatatct tgcaacttct tttctgataa atatagttga gtcggatgaa gatctgctat
     3901 attgactttc atttctttct cctcaaggga attcgatact cacttctgtt tgcctttaaa
     3961 tcgccattgg aagcggagct tgtcatagaa gggaaactcg ataaacagga ctcccaagcc
     4021 cacacagaga ctggcaagga cgtctgatgg gtaatgaact cccagataga ctcttgatac
     4081 cagcacactg actaggtaga ggccaaggac gatttgtacg atttttctcc agactggatc
     4141 tttaatccgc tgactaagaa taacaatcaa agtacctacc atcaaggtta cagccagaga
     4201 atgcccactt gggaaggaaa atcccttctc ctccaccaga tgtaagatag ctggtcgtgg
     4261 gcgctggtag atatttttaa aggtcacgat taaaagacct gccaaagcca gatttcccag
     4321 catgaagaaa ctttctatct tccatcgctt acgataaaag acaaaagctg taatgacaac
     4381 ccaagtgata atcactggga tatcaatcag acgtgtgagg gatcgaaaaa gaatagtcaa
     4441 ataatctggt aagtctcctc gaatggcagt ctgaatcgat tggtcaaaat tgaccaacat
     4501 ttcagggtaa aatttgacca tatagccaag aataacgaaa agtaaaaggg caaaactgcc
     4561 cttcattaaa aatgtttgtt tatctttcat agtgttttaa ggttggtttc aaaaggacgt
     4621 acaacaacca gaatgaaacg gaaaagatta caccctcaat caagttaaaa ggtaatacca
     4681 tggtcattag gtagttggaa agtcccaaaa tttttccaat atcaaagtta gcaaacttag
     4741 cgtacaaagg aacagcataa acatagttga gaaccaacat ggccaaggtt aaaccaacag
     4801 ttccagctag agagcctagt aggaaacgaa gggttgtccg ttcctttttc caaatcaaag
     4861 caaatacgat gacaaaaact cccaaagcta cgatattcat cggcaaacca atgtaagtat
     4921 tcactccttg gctgttaaga agcaatttca atagtgagcg aagcaagagc actcctagag
     4981 cagcaggcaa atccatgacc accagaccca caaggactgg caagatacta aattcgatct
     5041 tgaggaaaga tgccgctggt aaaagcggaa agtcaaagta catcagcaca aatgagatgg
     5101 ctgataaaat tgcaatggtc gaaagtcgac gtgtgtttgt cataacaggt tcctccaatt
     5161 ttctataaaa tcagaagaag ttggaaagga ttcctctatc tattctcact ttttatatcc
     5221 caaaagttcc ctcttactct attaaagaaa aacaaagcaa gtggttacaa tccggctata
     5281 aatctatcaa aacagacaaa gctattcttt cgtcttctcc catccagact atactgtcgg
     5341 ttgtggaatc tcaccacatc agcttgcgct cgcggacttc tttaaagaga aggaaataga
     5401 gatttttctt ttagactagg cgacaagctg taggcataac ttctggttag gcaaagcttg
     5461 tcaacaaagt ataaaaggaa aagatcattt cctagttaaa gtcaccgccg gtcgggaatc
     5521 acaccctgcc ctgaagactc ttttatcata acaaaaaacg cttgcaagcg caagcatttt
     5581 gttcattttc attttttatt tcaatttatc cacttcatag gtatggacga gctcaagacc
     5641 tgcaaagttc tgctggcgaa gagcctcata gacaatcatg cagacggtat tagacacatt
     5701 gagactgcgg acatgttcat cattcatggg aatacggaga gccttctcag gatgttctcg
     5761 cataaaatct tcaggtaagc ccttgtcttc acgtccaaag agaaaataat ggtcctcgtc
     5821 agtcgataaa tccacctcag aatagacttt ttccgcgaat ttcgaaatca gatagagttt
     5881 tcccttcatc tgagatatga aatcttccaa actctcgtaa aaataaatct ctagcttatc
     5941 ccagtaatcc aatccagccc gcttcatctt gcggtcatca ataggaaagc ccataggctt
     6001 gatgatgtgg aggggagaat tggtcgcagc gcaagtacgc gcgatattgc ccgtattttg
     6061 tggaatttga ggttcaaata atacaatgtg atttgtcatg acttgcttcc tttcaccatt
     6121 gcaaaaaaat agccacactg cccggagtca agctcagcaa acagcgtggt taaggcatcg
     6181 ttaacttacc tcacaacagg tttgaagtaa atcagcgaaa ctactttctt agtataacac
     6241 tttcagaatc attgtcaata gaaatgactt gattttttca attttttcaa gctatttcca
     6301 agggttgtaa aatcgtccct gattctgcaa gataagtagt aaactaacta ctaaaaacaa
     6361 ggttgccaag agcaaggtaa tatagtctcc ttttttcaag gcctgataac tataccatgt
     6421 gcgttttttc tctttcccaa agcggcgaag ctccatggca gtcgcaatgg tatcaatgcg
     6481 ttctagcgag ctaaaaatca agggcgtaat aatgagcaga ttgcctttga ttcgttgcat
     6541 aagagaagct ttcttggata attccatccc acgcgcctcc tgagacatct tgatagtaaa
     6601 gaattcttcc tgcaaatctg gaatatagcg caaggtcagg ctgacagaat aagcaatctt
     6661 atagggcaca ccaatttgat ttaaactgga agcaaactga ctaggatggg ttgtcatcaa
     6721 aaagataata gccagaggaa tggtgcaaag atacttaatg gccaaattta gcagataaaa
     6781 gagctcctgg ctggttagag tgtagacacc gattccctgc caaatcacac ttctctctcc
     6841 ataaagtcca accccatact cgggagaaaa gagatagacc atcaaaacgt ttaaaacggc
     6901 aaataccgtc gcaaaaacgg ctacaaagga aacatcttta aagcgaattt ctgataaata
     6961 gaggagaaag actgaaaaga tggcaatcag caagagcatt ctggtatcat agctaatcat
     7021 ggccgccaat gataccagaa tgaaaaagag aagtttccca gctcctgaca agcgatgaat
     7081 cacagtatct ctatgctggt aaccgattaa tttagcttgc atccctctct cctttctttg
     7141 taaaatgccg ttaaatccag tggatccaca tctagtttct tagccaagtt aaagatggag
     7201 gtttctttta gattggcttt tactaacagc tcaggatcgc tcaacagact ggctggaaca
     7261 gtatcggcaa tcaattctcc atccaccatg acaaggaccc ggtctgaata atccagcatc
     7321 aattgcatat catgggtaat catgacaatg gtatgccctt tttgatgtaa ctcttcgaga
     7381 aattccataa tctcagtata gttcttctga tcttgacctg cagtcggttc atctaggaga
     7441 ataatttcag ctcctaagac caaaattgaa gcaatggtga cacgtttttt ctgaccaaat
     7501 gacagggcag aaataggcca attacggaat tcataaagtc cacagatttt caaggtttca
     7561 tagactctcg tttcaatttc cttctcatcc acacctcgca aacggagccc tagagccacc
     7621 tcatcaaaaa tcatattggt tgaaatcatt tgattaggat tttgtagcac atatcctact
     7681 cgttccgccc gctctgcaac agaatcgcct tttatatcct gtttttccca aagatagcgt
     7741 ccttccgtct gaataaagct acttatagcc ttggctagag ttgatttccc tgctccattt
     7801 tttccgacaa tagcaatctt ttcacccttt ttaatatcta aatgtaggga ttttaaaatc
     7861 ggtctatcat cataagaaaa agatacttcc tctagtctaa agagtgactg caattctgag
     7921 gtttctttta ccagttccgt ctgtaactgg acttgccctt ttgagataga caagttatcc
     7981 agatctgcta attgttcttc cttgactaag tccacaccta attgacggag agtggttaga
     8041 taaaggggtt ctcgaattcc attttgagtc aataaatcag tcgcaagcaa ctggtcaggg
     8101 ctcccattaa aaaggatacg accatcgttt atcaagacaa tccgatccac agggcgatgc
     8161 agaacgtcct ccaaacggtg ctcgataatc agagtcgtcg tcccctcttc cttatgaatc
     8221 tggtcaatca attcgataat atcctgacct gacttgggat ctagattggc gagtggctca
     8281 tcaaacaaga gaatcggact ttcatcaatc aagacaccag ccagactgac tcgctgcttt
     8341 tgtccacctg acaaatcctg aggacgctga tccagtaaag gaagaaggtc cagcttttca
     8401 gcccatttat aaacacgacc tttcatctca tctagggctg tcacatcatt ttccagagca
     8461 aacgccaaat cttctgccac agacaagcca ataaactgcc catctgtatc ctgcaaaact
     8521 gtgctaacca gatgagactt atcatagatg ctcatatcaa aggctacttg accctttatc
     8581 aaaaattctc catatgtctg acccttgtaa atattgggaa taatcccatt caaacactga
     8641 cccaaggtag atttacctga cccagatggt ccaacaatta agactttctc tcccttgtaa
     8701 atggtcaagt ctatcccttg caaggtcggt tcttgttgtg tttcataccg gaaagagaaa
     8761 tccttccact caattatagc ttctttcatc ttactctctt cattcgcttc ttagacttct
     8821 attttatcat aaatcaagcc cttcttgcag tctcttctct taaaatctta gcgccaaaaa
     8881 gattcctatc ctagcttact tgcctaacta atctataaac atcgaaaaag actagttgcc
     8941 cagccttccc catcatttta tactcttcga aaatctcttc aaaccacgtc agcttcgcct
     9001 tgccgtaggt atggtt
//
LOCUS       DAVBDG010000072         8864 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBDG010000072 DAVBDG010000000
VERSION     DAVBDG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.073"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(221..850)
                     /locus_tag="VQ526_001770"
     CDS             complement(221..850)
                     /locus_tag="VQ526_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2555468.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(895..1134)
                     /locus_tag="VQ526_001771"
     CDS             complement(895..1134)
                     /locus_tag="VQ526_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555469.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(1284..1550)
                     /locus_tag="VQ526_001772"
                     /pseudo
     CDS             complement(1284..1550)
                     /locus_tag="VQ526_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1575..1886)
                     /locus_tag="VQ526_001773"
     CDS             complement(1575..1886)
                     /locus_tag="VQ526_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2555470.1"
                     /translation="MDWDFADTCWEKDPREYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHSELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(1811..2053)
                     /locus_tag="VQ526_001774"
     CDS             complement(1811..2053)
                     /locus_tag="VQ526_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2555471.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRILENTNMWLLTI"
     gene            complement(2054..2686)
                     /locus_tag="VQ526_001775"
     CDS             complement(2054..2686)
                     /locus_tag="VQ526_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555472.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(2700..3695)
                     /locus_tag="VQ526_001776"
     CDS             complement(2700..3695)
                     /locus_tag="VQ526_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2555473.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(3692..4390)
                     /locus_tag="VQ526_001777"
     CDS             complement(3692..4390)
                     /locus_tag="VQ526_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2555474.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQSKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4467..5477)
                     /locus_tag="VQ526_001778"
     CDS             complement(4467..5477)
                     /locus_tag="VQ526_001778"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2555475.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(5461..6468)
                     /locus_tag="VQ526_001779"
     CDS             complement(5461..6468)
                     /locus_tag="VQ526_001779"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2555476.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6455..7408)
                     /gene="mvaD"
                     /locus_tag="VQ526_001780"
     CDS             complement(6455..7408)
                     /gene="mvaD"
                     /locus_tag="VQ526_001780"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2555477.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(7390..8268)
                     /gene="mvk"
                     /locus_tag="VQ526_001781"
     CDS             complement(7390..8268)
                     /gene="mvk"
                     /locus_tag="VQ526_001781"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2555478.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8390..8512)
                     /locus_tag="VQ526_001782"
     CDS             complement(8390..8512)
                     /locus_tag="VQ526_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
                     /protein_id="HET2555479.1"
                     /translation="MTTGWFQVNGRWYYAYSSGALAVNTIVDGYSVNYNGEWVR"
BASE COUNT         2588 a         2074 c         1579 g         2623 t
ORIGIN      
        1 atttctaaca atgttttaga agtagaggtg tactattata gtttcaatct actatacaag
       61 acgaaattga agaaatcttt ccattataaa acgcatatta tcaaggtttt taaacaccta
      121 ataatatgcg ttttttccta ttttaaagac ttttatccac ctattataca aagttaagta
      181 tccatcacag tttacaatta gccttcatta cctaacccat tcaccatcct tcaagagagt
      241 aacctttaag aaccgaataa ataaatggca aatttcgttc atttaattta actgcactgt
      301 taatttgatt agctccatct cctaaagtat gcactcctac tacacggtga ctagcatcat
      361 aaactgtaga tccactagac ccctctaaag tatcaacttg gtaatccaag aacatgccat
      421 catcacttaa aacttgtttt ttatctgtat acatttgatg aattttaaaa ttatatgata
      481 gatagcctgt gatagtcaca gttattcctg tcaaattttt ttgactagta ggaagaccca
      541 aagtccctaa ttttgcacca atgggctctt ctagaattaa taaagccaag tcatattccc
      601 ttgcatcctt agaatttaaa tttctaaatt ccttcaaata acgaacttcc tttactttga
      661 tctttccaaa tagttcttga cttggactaa cagccggaag aacataaata tcatccgctt
      721 ctttcccata gtcatgtctg tagtagttgt gagccgctgt aattaacaca ttatctttaa
      781 taaaattcgc acttcccctc ccttctacat tcattccatc ggctactcca taatacttag
      841 ataaaaccat agctgaagtc gaatacggat actgtaaagt attatcaatt ttaatcaaat
      901 catcattacc gataatactt ctgattgctt ttggtagtat gaaccatacg ttggtgaaat
      961 ctcagataat gaagaatcat tagactctgg acctttttct agtgtctcac ttacctcata
     1021 ttcttcacct gtactagaaa taacactcaa agcagatact gtcgataact ggctagccaa
     1081 taaagtactc gcaataattg aaatacccaa ttttttataa acagttttct tcattattgt
     1141 atcctcctaa tgtaattata gcgtactatt ctaaatttct taatctacta tagaatcaag
     1201 aaatctacca ccttctttaa ataccctcca ttatcacata aacaggtaaa cttttcaatt
     1261 aatgactgcg cttttcaatc acgctagagg tacttgcttg cttctttgat actaagttca
     1321 gccattcttt ccttgttttt ctcaataaag catgttaccc aagtgggatt cgttttggag
     1381 tagtctcgca gagtccagcc aatggcttta ttgataaaaa attctgtttg gttcaagtta
     1441 tgaagtagaa tcttttccat taattgagta ttggtcttct cttttcttaa caactggtgg
     1501 tcaatagcga cacgtctcag ccagatatta tctgataggc tccattttat actcaatgaa
     1561 aatcaaagag caaactagga agctagccgt agattgctca aaacactgtt ttggggttgc
     1621 agatagagct gacgtggttt gaagagattt tcgaagagta ttaagattat ttcttctagt
     1681 tcagagtgtt catacaccaa actccctact actcgatcta ggatatctac cgtgtcccac
     1741 caggattttg tcacgactaa ctgctctagc ttaggcaaat cggtttcctt tagataagac
     1801 tgcattgctt tcaaatagtt agcagccaca tattggtatt ctctaggatc cttttcccag
     1861 caagtgtctg caaaatccca atcgataatc tttgtttttt tcgcttctgg aaaatatttt
     1921 tatagagttt atttctttca ggcaccgcaa tacctagaaa agaaaattga tggcgcatat
     1981 aggcttccat ggaccttgct tttttagagt cttttgctgc ttctagctcc tcaagtaaat
     2041 ctgctaaact catctaaaac tcctcttgcc ccaccaaatg gtgctgaaag gcatagacag
     2101 ccgcctgggt acgatcgctg acttcaagtt tggcaagaat attggacacg tgggtcttga
     2161 ccgtcttgag agagataaag aggtcatctg cgatgcgctg attttcgtag cccttggcga
     2221 tgagttggag aacatctcgc tcacgcgcag tcaattcttc atgaagttcc atatgattgc
     2281 ggtggtattc aaccttcttg ctaacctctt gctcaatggc cagctcgcca gcagctacct
     2341 tactgacggc atgaagcaat tcatctgcac tagaagtctt gagcatatag cctttggcac
     2401 cagcatctaa gactggcatg attttttcat tgtccaaata agaggtcaca atcaaaatct
     2461 tggcttcagg ccattcttta aggattgcta aggtcgcgtc aatcccattc atctcaggca
     2521 tgacaatatc catgacaatg acatctggac gcagttccaa ggccaagtca atcccttgag
     2581 acccgttgga cgcctcaccc acaacttcta catcgtcttg gaggtcaaag tagcttttca
     2641 agcccaatcg gaccatttca tggtcatcta ctagtaaaat tttcatcttt actcctttat
     2701 cattccttat ctaacagggg aatacggata tcaaccgcca gcccttgctt gggagctgtc
     2761 aagagttgaa ctgttccagc catatcttca acccgctcct tgatatttcg cagtccataa
     2821 ctcaagtcgt ctaagctccc taactggaaa ccaatcccat tgtccaccac cttcagttgc
     2881 aattcaacat ctgtctgata gaggtagaca tctaggcaag atgcctgggc atggcggagg
     2941 gtattgctaa tcaactcttg caggatacgg aagatatgct cctcgatttt cttaggcaat
     3001 ttcgtcatat tctgcttgag actaacccta agatcactct tgtcctcaag ctcttttaag
     3061 agaatttgaa tcccttctat caagctcttc tgctccagtt caactggtcg caaatgcaag
     3121 agcaaaaccc gcaaatcctt ctgggcagtt tctaaaatag ctgtgacact ctgcaactgg
     3181 atctgcatct tttctctatc caatttcaaa gcctgctgac tgatacccga taaaatcatg
     3241 tgggccgcaa acaactcctg actgactgta tcgtgcaaat cccgagcaat tcgcttccgt
     3301 tccttctcga tgatttcctc ttcctgagca aggctatgat tttcagcttt ttgaagagct
     3361 tctgtcaaaa ggttaagttt acctgataag gacttgaaac tggcatccaa atctggatct
     3421 gcaacctgaa ccacttcttg ccctgccaat aaacgcttga gattagcctg catttttctt
     3481 agagaaagct cttcgatccc tcgccaaaac agggctaaga gacaggttat ggacatgctg
     3541 aaaaccaaca ataaaaagac aaatttttct gttttttcga catcgtgcaa aaagatagac
     3601 cagtcaaaat caagtatttc cagcaagctg tgggagaaaa aaaagacaaa caggaaggag
     3661 gtgagagcaa taatgacata ggcttgtttt ttcatcctct aaccacctcc acatcaccaa
     3721 tcatagtggt caagaaaatc ttgacactct tgttactctt gagatagtct tttgtttctt
     3781 gatgatagtg ttcattgcgg agggctcgct tggactggtt gaaaaaagtc aaatccccat
     3841 agagacagtt aacgctgaga ctgacttcca catctacagg tacgatgatt ttggtcgttc
     3901 ctaccatctt tctgaggata atgacattgt catgattggt taagatgacc ctctccagat
     3961 gaatagtgtc cttgcccatg aagcgaaaga gattgatatc atcgaattgg caagtctggt
     4021 agcttgaaaa atgatgaaga tttccaaacc aacgattttt ctccttctta accgtcacga
     4081 cctcttcaaa aaccaaattg gtctgctctt tttcctggtt catcatcggg taaagaagaa
     4141 agaggctata gataaccgca acaaaaatag ctagaatcac aaaaggattg agcataacga
     4201 tgaaaaagaa gagaatggtt gccgctacta aaagaagatt atttccctct ttaccagtgt
     4261 agtagcgaat caaaagcaaa aagaggaata gtatcagcag aaaacgcgaa aaatgctctg
     4321 ataccatcaa aatcagagct cctgtcagaa gacaggcttc gataaataaa aagattttaa
     4381 attttctcat aggttcatcc tctcccttct attttatcac aattcaaaaa agtcacctca
     4441 gtctgaggat ggaaaaaagg cgctggttac gcctttttca tctgatcctt tgcttctttt
     4501 aattttccat aaagaagata gtctactttt tgtagatctg ctatggtggc acagttaagg
     4561 gaacacataa tcaagcgtag gtctgctttc cagccttgga caatgccaat cacttcttca
     4621 actgtgtagg tttcaaccaa ttccagaacg gttcgtgaca atcccacaga cttagcacca
     4681 aaaaccaagc acttaatcat atccagcgga ttccgaaccc ctccactaac caagagttcg
     4741 accttatctt tccattcttg ggcattgaga agggcctgca tggtagactg accccattga
     4801 ttgaggtaat cacgctggcc actacgacgg ttttcgatat aggcaaagct ggtgccacca
     4861 cgacccgata ggtccactgt acgaacaccg aattcatagg ctctttcgat tgtcttggca
     4921 tccattccaa agcccacttc cttgaggaca ataggaacgg gaatttgctc gctataatct
     4981 gctagatgcg attgccagct tctaaacttc ctttctccct cgggcatgag taattcctgc
     5041 atgacattga catgcacttg caatagaaca ggattcatct cttctacagt ctgaagccct
     5101 aactcgacag gcttgtccaa tccaatattg gttccaagga ggagattggg atgactagac
     5161 ttgacagaaa aagaatcatc cgttggattt ttgagggctg cgctataaga acccgttaca
     5221 aataaaatac cacaggattc cgccacctga gccagctttt gattgatttc tcttccctta
     5281 ttacttccac cagtcatggc attgatataa aaaggaaagt cccactttcg accagcaaac
     5341 tctgtcgaaa gatcgatttc atccagattg taaagaggca aggaagaatg aatcagctcc
     5401 acctcatcaa agctattata ggaacttttc tgctcaaggg catagaggat atgctcgtcc
     5461 ttacgatttg tcgtcatgtc ctatcctttc ttgatataag agctcaatcc ccagatcggc
     5521 ccaacgattt tttaaggttt cggttgattg cgcatcaaaa ctcaaggcga tgccacagtc
     5581 accaccacca gcaccactac tcttggcaac ggcctgcaaa tcttgactgg cttctttcaa
     5641 ctgtctaagc aaaggcgtgt aaatatctgt actcaagcct tctaaaagct tgctggctac
     5701 ttctacttgc tcgataactt tttcggcttt cccctgctcc aaggcttcga ccaaagaaac
     5761 caccgtttct tttgaggaac ttaaaaaatt ttgattgata ttttgcttga tttgctggac
     5821 catgtgactc gatacagcca cttccttggt ccatcccact aagaaatcac attctaaagt
     5881 tggtttcact tgtgagataa aaaatcccca atcacgctcc agaactgtcg ccaagttttc
     5941 ttcttctaac caagcagcca ccttctggcg atcaaatgac tggtagagaa ccaaatcctc
     6001 tgccacaata caggcaaggt cgcccatgga accattgtct cctcgcttga gcaagacagc
     6061 gctagtcagc ttgaacaaga gattctgatc aaccgaaaga ttatagagag ccagtaaagc
     6121 cttgacaacc aagacaacga cgctgccact agaacctaga ccaaactttt tcccttctcg
     6181 ttccattttg ccacagattt ttagggaaaa aggtcttaaa ttctgaccgc gaacagcgag
     6241 gaagtctccc atcaaagcaa tcgtttcttg aatcaagctg tagtcaggat taggccttaa
     6301 gtccactgcg aaatcaaaca tatctgaata gatacggtag ctgtcagaaa aagcaatttc
     6361 agccctcata tagatgggaa tatcctttat caaagctaac tgccctggct ctaaaatagc
     6421 atattcacct gcccaataga gttttccgca agttttaaca gcaatcatct tgactcaaat
     6481 cctttgtttt tgacacaatc aagcgataac gctgaccgaa aatttctgat aaatgctcca
     6541 agtctttctc ctgacagaag accttaacat tgggaccagc atccatggta aagtagcagg
     6601 cctctccttt ctcacgaagc tggcgaacaa agtccatagc ctcataagag gcatccgtca
     6661 gataagaaaa ggctggacta gcagtctttg tcgtagcatg catagccagg gcatttttct
     6721 ccgttaattc tccaatcttg gcaaaatcat tttccttgag ataaatcagc atatcctgat
     6781 agtccttctc agactgacga acccagtcgt cgaaagtcgt cgaggtttcc acacaaagtt
     6841 tcatcccgtc acggctagag attggttttt tcttgtcctc tagcaccaac ataatcatag
     6901 ctagtttcaa gtctgtctct acagggtaaa tttctccact atccttatcc caggctccta
     6961 gtggtccata aaaactccga gaagaagagc ctgaggcaaa cttggcttcc tgcgctaact
     7021 gacttctatc caatccaagc ttgaaataag cattacaagc cttgaccagg gcggacaaac
     7081 cactagaact tgaggacagg cccgctgccg taggcatatt gttttgagta tcgatacgga
     7141 caaagccctc accagctgga cgataacggt caataatctt actcatcttg gcatgctcga
     7201 cctcattttg tagctgacca ttgatgtaaa attcgtcagc tgttacattg gctggtaaag
     7261 gcgacaaggt cgtctctgta tacatatttt ccaaagttag agaaatactg ctagtagcag
     7321 gcaccatctc tttttctttt ttctttcccc aatatttgat aatagcaata tttgcgtagg
     7381 aacgtactgt tacaggctct ctatccatgt ctgaacagct cctttctctt ctaatctttc
     7441 tgctagttct tgtgcgtgtg tcaaattggt taccaaggct atgatacaac ctcctagccc
     7501 accaccgctc atcttggcac ccagagcacc atggctaaga gtcgtttcaa ccaaaaagtc
     7561 tgcctcaggg ctactgactc caatttcttt taaatgtaaa tgcgcttgac tgaggatttg
     7621 tcccagtcct tcagcatctt tttgtgaaat cgcaacttct gcttgctggg ttaattctcc
     7681 caaggcatgc aaaaacggta gggcatcctt gcccttattt tgaaccactt ggatggcttc
     7741 acgagtatga ccataaacac ccgtatcggc aatcaccaaa taggcggata aatccatctc
     7801 aagttctgta aatcctacgt tcttgataaa gcgaataggt tggtcactga gacaggtctt
     7861 agcatccaaa ccactaggat tcatatgggc aatcatttca gctcgattga ccaagatttc
     7921 tagtacatca tgaggcagat cagcctgata gtagtcaaat accgcacgaa tggccgctat
     7981 gctgatagcc gctgacgaac ccatcccccg tttctcaggg atagccgagt caatctcaca
     8041 acgaatgcag gcttctgtga tatccaaata ctccagtgag gcataaaccg ccatggacaa
     8101 ggtatcctcc tcataaaggc gccaaggact ctctgcagga actaccttac aggtcacctc
     8161 cacctccaaa agaggcaggg aaatggcagg ataaccgtaa acgaccgcat gttcccctat
     8221 taaaattatc ttactatgtg cctgaccgac accaactttt tttgtcattt tttcctttta
     8281 ctagacgaaa aaacgtctta tttttcatac aagtattaat tctttcctat ctattttatt
     8341 atattttcac aaaaaaagcg attgtttcca ttcacaatcg cttctttcat taccgaaccc
     8401 attcaccatt ataattgaca gaatagccat ctacaatcgt attcactgcc aaggcacctg
     8461 agctataagc gtagtaccat ctgccattga cctggaacca acctgtcgtc atagaacgat
     8521 gaaagaaact ccataccatt aagtaaagag gaaagtcatg agggagcatg cgtcattgac
     8581 aacctatttt agtgacgtac aaagtttcat taacaagtac tcgtttcggc catttatagg
     8641 tgcggtgttt gcagaaatag ggttcaatct tcgcccattc ttgatcgttt aaatcagtat
     8701 catatgcttt gcgtgtcata actctagttt aacatttttt tgtgaataca ggttctaaat
     8761 aatcgaccac gaaaatttct taagtggaaa acgccttatg aagtatgcta cgggaaactt
     8821 atgcacttaa tttgacaact caatatgtaa aaatatatac tata
//
LOCUS       DAVBDG010000073         8669 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBDG010000073 DAVBDG010000000
VERSION     DAVBDG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.074"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            192..1211
                     /gene="galE"
                     /locus_tag="VQ526_001783"
     CDS             192..1211
                     /gene="galE"
                     /locus_tag="VQ526_001783"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2555480.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTVTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1213..2166
                     /locus_tag="VQ526_001784"
     CDS             1213..2166
                     /locus_tag="VQ526_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2555481.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(2228..2434)
                     /locus_tag="VQ526_001785"
     CDS             complement(2228..2434)
                     /locus_tag="VQ526_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2555482.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            2486..2962
                     /locus_tag="VQ526_001786"
     CDS             2486..2962
                     /locus_tag="VQ526_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2555483.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            2971..3642
                     /locus_tag="VQ526_001787"
     CDS             2971..3642
                     /locus_tag="VQ526_001787"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2555484.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            3773..4111
                     /locus_tag="VQ526_001788"
     CDS             3773..4111
                     /locus_tag="VQ526_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2555485.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            4114..4743
                     /locus_tag="VQ526_001789"
     CDS             4114..4743
                     /locus_tag="VQ526_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2555486.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            4983..6134
                     /locus_tag="VQ526_001790"
     CDS             4983..6134
                     /locus_tag="VQ526_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2555487.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            6452..7201
                     /gene="truA"
                     /locus_tag="VQ526_001791"
     CDS             6452..7201
                     /gene="truA"
                     /locus_tag="VQ526_001791"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2555488.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQIDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IALILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            7191..7964
                     /locus_tag="VQ526_001792"
     CDS             7191..7964
                     /locus_tag="VQ526_001792"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2555489.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKVFVYRAIAQADQYGVRQYEANKNN"
     gene            7942..8403
                     /locus_tag="VQ526_001793"
     CDS             7942..8403
                     /locus_tag="VQ526_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2555490.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLLDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMAGMIVGFILSQSIKKIK"
BASE COUNT         2437 a         1626 c         1948 g         2658 t
ORIGIN      
        1 aggttgtgta tagaactgac gaagtcagta accataccca tggcaaggcg acgttgacgt
       61 ggtttgaatt ggattttcga agagtataag cattttactg aaattttatc aggtagttta
      121 ggatggcaaa tgacattccc tgtcaaaaat ggtaaaataa gaaaaaataa tctgagaatc
      181 gaggaaaaaa gatgcaagaa aagattttgg taactggtgg tgccggtttt attggaaccc
      241 acactgttat tgaattgatc caagcaggcc atcaagttgt tgtggtggat aatcttgtca
      301 atagcaatcg taagagttta gaagttgttg aaagaatcac aggagttgag attcctttct
      361 atgaggcaga tattcgtgac acagttaccc tcagagatat tttcaagcag gaagaaccga
      421 ctggtgtcat tcactttgct ggtttgaagg ctgttggcga atcaacacgt atccctcttg
      481 cctactatga caacaatatc gctggaactg tcagcctttt gaaagccatg gaagaaaaca
      541 actgtaaaaa catcatcttc agttcttctg cgacagttta cggggatccg cacacagtgc
      601 ccatcttgga agatttccca ctttcagtga ccaacccata cggtcgtacc aagctcatgc
      661 tagaggaaat tttgaccgat atttacaaag cagactcaga atggaatgtt gtcttgcttc
      721 gttactttaa cccaatcgga gcccatgaga gtggtgattt gggagaaaat ccaaacggta
      781 ttccaaacaa tctcttgcca tatgtgactc aagtagccgt tggaaaatta gagcaagtgc
      841 aagtgtttgg agacgattac gatacggaag atggaacagg tgttcgtgac tatatccacg
      901 ttgtcgattt ggctaagggt cacgttgcag ctttgaaaaa aatccaaaaa ggttcaggac
      961 taaacgttta taaccttgga actggtaaag gttactcagt tcttgaaatt atccaaaaca
     1021 tggaaaaagc ggtgggacgt cctattcctt accgcatcgt agaacgtcgc ccaggtgata
     1081 tcgctgcctg ctactcagac ccagcaaaag ctaaagcaga actcggttgg gaagcagaac
     1141 tcgacatcac ccaaatgtgt gaagacgcat ggcgttggca aagcaagcat ccaaatggat
     1201 ttgaagacta agatgatgat ttcaatcatc gtcccttgtt taaacgaaga ggaagtactt
     1261 cctctttttt atcaggctct ggaagcttta cttccagatt tggaaacaga aatcgagtat
     1321 gtctttgtcg atgatggatc aagtgatggg accttggaac tcttaaaggc ctatcgggag
     1381 caaaatccgg cagtccatta tatttctttc tctcgaaatt ttggtaaaga agcagccctt
     1441 tatgcaggct tgcaatatgc gacaggagat ttggtggtgg tgatggatgc agacctccaa
     1501 gatcctccta gtatgttgtt tgagatgaaa aatgtactag acaaaaatgt agacttggac
     1561 tgcgttggga cacggagaac tagtcgggag ggagaaccct tctttcgcag tttctgtgct
     1621 gttctctttt atcgcctcat gcaaaaaatc agcccagtag ctctgccgtc gggtgtccgt
     1681 gattttcgta tgatgagaag gtctgtggtc gatgccattt taagcttgac tgagtccaat
     1741 cgtttttcta agggactctt tgcctgggtc ggctttaaaa cccactatct ggactatcca
     1801 aatgtcgaaa ggcaggctgg caagaccagt tggagtttta ggcaactctt tttttactcc
     1861 attgaaggga ttgttaattt ttcagatttc cctttgacta tagcctttgt agctggtctc
     1921 ctatcttgtt ttctttctct gctgatgacc ttttttgttg tggttcggac cctcattttg
     1981 ggcaatccga catctggttg gacctctctg atggctgtta ttctctttct tggaggcatt
     2041 caactcttga ccattgggat tctcggtaag tatatcagta agatttattt agagactaaa
     2101 aaaagaccac tttatcttat caaagaaaaa agtgaccttc ctgattttac agaaaaaata
     2161 aagtgaaaag actataattt tacatggaaa tgtgctaaac tagagggagt ggattatcgc
     2221 cattgattta ggcttcctgg tttccaatca gggcgcgagt tgggcaattt ttaacagcct
     2281 ctaagacatc ctgactagga gaaatttctt tttccagttg gtcagggtca tcgtaaaaac
     2341 gcacgattcc attatcgtgg taatcaaata aatcagaata agtttggcaa agtccacagg
     2401 cgatacatcg ttcaggtata agtgtgattt tcatgttcat atttatattg taataagaaa
     2461 agtaaaaaaa aacaaggagt aaggtatggc aaaagaaccg tggcaagaag atatttatga
     2521 tcaagaagaa tcaagagcag ggcgtcggca tcgaaaccac ggaggggctg ataggatggc
     2581 taatcgtatt ttgacgatcc tagctagtat tttctttgta attgtggtgg tgatggtcat
     2641 cgttctcatc tatctatcat cgggggggag taatcgcaca gcagccttaa aagactttca
     2701 tgattctgat gcaagtgtag tacaaatctc atcttcaagc agttctcagc ctgagcagag
     2761 ttcagagcca gaatctactt ctagtagttc agaagaagct gctaatcctg aaggaacgat
     2821 taaagttctc gcaggagaag gggaagcagc tattgccgct cgtgcaggaa tctccattgc
     2881 tcagttagag gccttgaatc ctgggcacat ggctacagga tcttggtttg ctaatccagg
     2941 tgatgttata aaaataaaat aggagccgat atgaaaacaa ttcaaattgc tattgatggt
     3001 cctgcttcca gcggtaagag tacggtcgca aagattattg ctaaggattt tggattcacc
     3061 taccttgata caggagctat gtatcgtgca gcgacctata tggctcttaa gaaccaatta
     3121 ggagttgaag aagtcgaagc ccttctagcc ttgttggacc agcatccaat cagctttgga
     3181 cgttcagaaa ctggagacca gcttgttttt gtaggagatg tggatattac ccatcctatc
     3241 cgtgaaaatg aagtgaccaa tcatgtttct gctattgcag caattcctga agtgcgtgag
     3301 aaactggttt ctctccaaca agaaattgcc cagcaaggcg ggattgtcat ggatggtcgc
     3361 gatattggaa ctgttgtatt gccacaagca gaattgaaaa ttttcctagt agcttctgtt
     3421 gatgagagag cagagcgtcg ttacaaggaa aatattgcca agggaattga aacagacctt
     3481 gaaaccctaa aaaaggaaat tgctgcgcgt gactacaagg atagtcatcg tgagacttct
     3541 cctctcaaac aagcagagga tgctgtctac cttgatacaa ctggtttgaa cattcaagaa
     3601 gtagttgaaa aaatcaaagc agaagctgaa aaaagaatgt aaatgtagaa aagccgatag
     3661 attgatatcg gtttttttct aaatttgtca aaatgctgat tttagggtat aatagttgct
     3721 gttcgagaaa ttttgatttt caaagaatag tgaatgatga taaggagaaa ccatgaacaa
     3781 tttaccaaat tgtcccaaat gtaactcaga gtatgtctac gaagacggtg ccctactggt
     3841 ttgcccagag tgtgctcatg agtggaatcc tgctgaagtt gcagaagtag aagaaggtct
     3901 tgtcgctatc gatgccaacg gaaataaatt gactgatggt gatacagtaa ctctcatcaa
     3961 ggacttgaaa gtaaaaggtg cgccaaaaga tttgaaacaa gggacgcgcg tgaaaaatat
     4021 ccgcatcgta gaaggcgacc acaatatcga ctgtaagatt gatggttttg gtgccatgaa
     4081 acttaaatca gagtttgtga ggaagattta agcatgtcaa aacactataa acttgtattt
     4141 tatagccgta tcttcttgtt tctagcggct tttacgggag tttatcttga aattactagg
     4201 catggtggtt ttgggatgct tctctattac acggttctgt ccaacctctt ggtaactatt
     4261 tttacccttt atcttctaaa ggttatgagc cgtgtaggtg aaaattggca aaggccaagt
     4321 ctcttgcgct taaaaggtgg ggtcaccatg agcatcatga ttacctgtgt gatttaccat
     4381 ttcctcttgg cgcccattgc gactaatttc tataccctag aaaatttcct ttgccactat
     4441 atcgttccca tctggttttt agcggatacc ctcttttttg acaaacaggg tcaatacaag
     4501 atttgggatc cagcagtgtg gaccatttta ccctttctgt atatgatgtt tgctcttttt
     4561 aatggcttgg ttctaaaact caatattcca aatgccaagg ataatccttt cccttacttc
     4621 tttttgaatg tgaacaaggg ttggaatgtt gtgtttaagt ggtgtctgat tatctttgtt
     4681 gcctatatgg tagcaggatt tattttctac tttatcaagc aaatcaagag aaagtcatcc
     4741 taatactctt cgaaaatctc ttcaaaccac gtcagtttcc atctgcaacc tcaaaacact
     4801 gttttgaaca acctgcggct agcttcctag tttgctcttt gattttcatt gagtataaga
     4861 tacaagggat tcattttcga atttaagaag gagtctacaa accaatgagg cttcttcttt
     4921 tcttgcttat ttgataacta tcaaaaaagt agaagattaa gagaaatata gaaagagatt
     4981 tcatgaaaca atttttagaa cgggccagca ttttggctct ctccctcgtt ttgattacct
     5041 ccttttccat ttcgagtgcc ctaccagcca tgtttgacta ttatcagggt tattctaagg
     5101 aacaaattga gctcttggtg agcttgcctt cctttggaat tatgatgatg ttactgctaa
     5161 atggtttctt agaaaaaata tttcctgagc gcttacagat tagtttgggc ttgctgattt
     5221 tatcattgag cggtacagct cccttctggt accaagccta tccctttgtc tttggaacac
     5281 ggcttctctt tggtttgggt cttgggatga tcaatgccaa ggccatttct attatcagtg
     5341 aacgctacca aggaaaaagg cgaattcaga tgttagggct acgcgcttct gcagaggtcg
     5401 ttggagcttc tctcattacc ttggccgtcg gtcagttgtt ggcctttggt tggacagcta
     5461 tctttctagc ctatagtgct ggatttttgg tgctgcccct ttatctgctc tttgtccctt
     5521 atggaaaatc aaagaaagaa gtcaagaaaa gagcgaagga agcaagtcgt ttaactcgag
     5581 aaatgaaagg cttgattttt accttagcta tcgaagcggc agttgtagtt tgtaccaata
     5641 cagctattac catccgtatt ccaagtttga tggtggaaag aggattgggg gatgcccagt
     5701 tatctagttt tgttcttagt atcatgcagt tgatcgggat tgtggctggg gtgagttttt
     5761 ctttcttgat ttctatcttt aaagagaaac tgctcctctg gtctggtatt acctttggct
     5821 tggggcaaat tgtgattgcc ttgtcttcat ccttgtgggt ggtagtagca ggaagtgttc
     5881 tggctggatt tgcctatagt gtagtcttga cgacggtctt tcaacttgtc tctgaacgaa
     5941 ttccagctaa actcctcaat caagcaactt catttgctgt attaggctgt agtttcggag
     6001 cctttacgac cccatttgtt ctaggtgcaa ttggcttact aactcacaat gggatgttgg
     6061 tctttagtat cttaggaggt tggttgattg taatctctat ctttgtcatg tacctacttc
     6121 agaagagagc ttaggattga ttcctaagtt tttcttttga tactcaatga aaatcaaaga
     6181 gcaaactata gttgattgag tttggaatag tacgctgtag ctgctaaaat atttctagaa
     6241 attaatttga ctttcctaat agagttattc atatcttatt tcaatctact atagaaagct
     6301 agccgcaggt tgctcaaaat agtgttttga ggttgcagat ggaaactgac gtggtttgaa
     6361 gagattttcg aagagtatga gatttttata cccatacaat atttgtttta ggtccttgtt
     6421 tacagactaa tttcttgata aaatagaggt tatgacgaga tataaagcaa ctatttccta
     6481 tgatggttat gcctttgctg gctttcagcg ccagtctcat gcgcgtagcg ttcaggaaga
     6541 aattgaaaaa accttgacca gattaaataa agggcaagcc attactgttc acggtgctgg
     6601 taggacagat agtggggttc atgccctggg acaggtcatt cattttgacc tgccttatca
     6661 gattgatgag gagaaactcc gttttgcctt ggatacccag tctcctgaag atattgatgt
     6721 gatttcgatt gagcttgtgg cagatgattt tcattgccgt tatgccaagc atagcaagac
     6781 ctatgagttt attgtggata gaggacgtcc caaaaatccg atgcgccgtc actatgccac
     6841 ccactttccc tacccactcg atgtggaacg aatgcagatt gcaatcaaaa agctagaggg
     6901 aacccatgat tttaccggtt ttacagcctc tgggactagt gtagaggata aggttcgcac
     6961 catcacagaa gctagtttaa tagtcgatga gacaggacaa tttttgacct ttaccttttc
     7021 cggaaatggt ttcttgtata aacagattcg caatatggtg gggactcttc tcaaaatcgg
     7081 taacaaccgc atgccagtag agcagattgc tctcatcttg gagaagaagg acaggcaact
     7141 tgcaggtccc actgcagcac caaatggttt gtatttaaag gagattcgtt atgaagaata
     7201 atcgtatttt agcactttct ggaaatgata tttttagtgg tggtggactg tcagctgatt
     7261 tggctaccta taccttgaac ggcttgcatg ggtttgtagc agtgacttgt ttgacagcct
     7321 tgacagaaaa aggatttgaa gtctttccaa ctgatgatac catttttcaa catgaattag
     7381 atagcttgcg tgatgtggaa tttgggggaa ttaagattgg tcttctccct actgtcagtg
     7441 tggctgagaa ggccttggac tttatcaaac aacgcccagg agtacctgtg gtgttggatc
     7501 ctgtcttggt ctgcaaggaa acgcatgatg tagctgtcag tgagctctgc caagagttga
     7561 ttcgcttttt cccttatgtc agtgtgatta cgcctaatct cccagaagca gaattattat
     7621 ccggtcagga aattaaaacc ttggaagaca tgaaaactgc agcgcagaaa ttgcatgatt
     7681 taggagctcc agcagtcatt atcaagggag gcaatcgtct tagtcaggac aaggctgtgg
     7741 atgtctttta tgatggacag acctttacta tcctagaaaa tccagttatc caaggccaaa
     7801 atgctggtgc aggttgtacc tttgcctcta gcattgccag tcacttggtt aaaggtgata
     7861 aacttttgcc agcagtagaa agttctaagg ttttcgttta tcgtgctatt gcacaagcag
     7921 atcagtatgg agtaagacaa tatgaagcaa acaaaaacaa ctaaaatcgc ccttgtatcc
     7981 ctattaaccg ccctttctgt ggttctaggt tatttcttaa aaatcccaac acctacagga
     8041 attctaactc ttttagatgc tggtgtcttc tttgcggcct tttactttgg tagtcgtgaa
     8101 ggagcggtag tcggaggact agcaagtttc ttgcttgacc tcttatcagg ctaccctcag
     8161 tggatgtttt ttagcttggt caaccatggc ttgcagggat ttttcgcagg atttaaagga
     8221 aaaagtcagt ggttaggcct tattttagca actattgcca tggtaggggg ctacgccttg
     8281 ggttctactt tgatgaatgg ctgggcagca gccctcccag aaattctacc aaattttatg
     8341 caaaatatgg cagggatgat tgtaggattt attcttagtc aaagtatcaa gaagattaag
     8401 taatactctt cgaaaatctc ttcaaaccac gtcggcttcc atctgcaacc tcaaaacact
     8461 gttttgagca gcctgcggct agctttctag tttgcacttt gattttcatt gagtataaaa
     8521 agatcttggg aaaagactca atttcagtag aaaaggaagt aaatcttccc acattaaaac
     8581 gcatattacc aaggttctta tgaactgcac cccaaaagtt agacagaaaa aatctaactt
     8641 ttggggtgtt tttattatga aattgagtt
//
LOCUS       DAVBDG010000074         8230 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBDG010000074 DAVBDG010000000
VERSION     DAVBDG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.075"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            89..1027
                     /gene="msrB"
                     /locus_tag="VQ526_001794"
     CDS             89..1027
                     /gene="msrB"
                     /locus_tag="VQ526_001794"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2555491.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            1085..2809
                     /locus_tag="VQ526_001795"
     CDS             1085..2809
                     /locus_tag="VQ526_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555492.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            2811..4559
                     /locus_tag="VQ526_001796"
     CDS             2811..4559
                     /locus_tag="VQ526_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555493.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEVSREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            4816..6075
                     /locus_tag="VQ526_001797"
     CDS             4816..6075
                     /locus_tag="VQ526_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2555494.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETATTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    6151..6286
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            6325..6825
                     /locus_tag="VQ526_001798"
     CDS             6325..6825
                     /locus_tag="VQ526_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2555495.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            6901..7269
                     /gene="rplL"
                     /locus_tag="VQ526_001799"
     CDS             6901..7269
                     /gene="rplL"
                     /locus_tag="VQ526_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2555496.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            7456..7629
                     /locus_tag="VQ526_001800"
     CDS             7456..7629
                     /locus_tag="VQ526_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555497.1"
                     /translation="MQKKGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVEILVLEGQS"
     gene            7626..8222
                     /locus_tag="VQ526_001801"
     CDS             7626..8222
                     /locus_tag="VQ526_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2555498.1"
                     /translation="MKRITASQYQTSERYYKLPKLLFESERYKNMKLEVKVAYSILKD
                     RLELSLNSGWIDEEGAVYLVFSNSKLMKLLGCSKSKLLSIKKTLREYGLIDEVQQSSS
                     EKGRMANKIYLGELEHEIPLVLHSDGASVKNRLGQSQKQTGPFLNSAPSETEESETDS
                     LFIEDEEERDTQPILKRKVEKVTKYDRDYIWGLVQDQF"
BASE COUNT         2423 a         1609 c         1814 g         2384 t
ORIGIN      
        1 aaccatacct acggtaaggt gacgctgacg tggtttgaag agattttcga agagtattag
       61 ttaaaaacgt gataaaggag aaataaaaat ggcagaaatt tatctagcag gtggttgttt
      121 ttggggccta gaggaatatt tttcacgcat ttctggagtg ctagaaacca gtgttggcta
      181 cgctaatggt caagtcgaaa cgaccaatta ccagttgctc aaggaaacag accatgcaga
      241 aacggtccaa gtgatttacg atgagaagga agtgtcactc agagagattt tactttatta
      301 tttccgagtt atcgatcctc tatctatcaa tcaacaaggg aatgaccgtg gtcgccaata
      361 tcgaactggg atttattatc aggatgaagc agatttgcca gctatctaca cagtggtgca
      421 ggagcaggaa cgcatgctgg gtcgaaagat tgcagtagaa gtggagcaat tacgccacta
      481 cattctggct gaagactacc accaagacta tctcaggaag aatccttcag gttactgtca
      541 tatcgatgtg accgatgctg ataagccatt gattgatgca gcaaactatg aaaagcctag
      601 tcaagaggtg ttgaaggcca gtctatctga agagtcttat cgtgtcacac aagaagctgc
      661 tacagaggct ccatttacca atgcctatga ccaaaccttt gaagagggga tttatgtaga
      721 tattacgaca ggtgagccac tcttttttgc caaggataag tttgcttcag gttgtggttg
      781 gccaagtttt agccgtccga tttccaaaga gttgattcat tattacaagg atctgagcca
      841 tggaatggag cgaattgaag ttcgttctcg ttcaggcagt gctcacttgg gtcatgtttt
      901 cacagatgga ccgcgggagt taggcggcct ccgttactgt atcaattctg cttctttacg
      961 ctttgtggcc aaggatgaga tggaaaaagc aggatatggc tatctattgc cttacttaaa
     1021 caaataaaac agagagtggg gcttcccact ttcttcattt ctagaatatg aatagaaggg
     1081 atttatgaaa cacctattat cttacttcaa accctacatc aaggaatcaa ttttagcccc
     1141 cttgttcaag ctgttagaag ctgtttttga gctcttggtt cccatggtga ttgctgggat
     1201 tgttgaccaa tctttacctc agggagatca aggtcatctc tggatgcaga ttggcctgct
     1261 ccttatcttt gcagtaattg gcgttttagt ggccttgata gctcaatttt actcagcaaa
     1321 ggcagcagta ggttttgcta aggaattgac aaacgatctt tatcgtcata ttctttcctt
     1381 gccaaaggac agcagagacc gtctgacaac ttctagtttg gttactcgct tgacttcgga
     1441 tacctaccag attcagactg gtatcaatca attcctgcgt ctctttttac gagcgcccat
     1501 tatcgttttt ggtgccattt ttatggctta tcgaatctca gctgagttga ctttctggtt
     1561 cttagtcatg gttgccattt tgaccattgt cattgtaggg ttatctcgat tggtcaatcc
     1621 tctctacagt agtctcagaa agaaaacgga ccaactggtt caggaaacgc gccagcaatt
     1681 gcaagggatg cgggttattc gtgcttttgg tcaagaaaaa cgagagttac agatttttca
     1741 aacccttaac caagtttatg ctagattaca agaaaagaca ggtttcttgt ctagtttatt
     1801 aacacctctg acctatctga ttgtcaatgg aactcttctc gttattatct ggcaaggcta
     1861 tatttcaatt caaggaggag tactcagtca aggtgctctc attgctctta tcaattacct
     1921 cttacagatt ttggtggaat tggtcaagct agccatgctg atcaattccc tcaaccagtc
     1981 ctatatctca gccaagagaa tcgaggaggt ctttgcggaa tctcccgaaa acatccattc
     2041 agaattagaa caaaagcaag ttaccagtgg tcgggtttta caagtccaag aattgacttt
     2101 tacctatcct gatgcggccc agccttctct gagagacatt tcctttgata tgactcaagg
     2161 acaaatcctt ggtatcattg gggggactgg ttctggtaaa tcaagcttgg tgcaactctt
     2221 acttggactt tatccagtag acaaggggaa cattgacctt tatcaaaatg gacgtagtcc
     2281 tcttaatttg gagcagtggc ggtcttggat tgcctatgta cctcaaaagg tcgaactctt
     2341 taagggaact attcgttcca acttgacttt aggtttaaat caagaagtga ctgatcaaga
     2401 actctggcaa gccttggaga ttgcgcaagc taaggatttt gtcagtgata aggaaggact
     2461 cttggatgct ctagttgaag caggagggcg aaatttctca ggtggacaaa aacaaaggct
     2521 gtctatcgca cgagcagtct tgcgccaggc tccgtttctc atcctagatg atgcgacctc
     2581 agcactggac accattacag agtccaagct cttgaaagct attagagaaa attttccaaa
     2641 catgagctta attttgatct ctcaacgaac ctcgacttta cagatggcgg accagattct
     2701 cctcttggaa aaaggtgagt tgctagctgt tggcaagcac gatgacttga tgaaatccag
     2761 tcaagtctat cgtgaaatca atgcatccca acatggaaag gaggactaga atgaaacgac
     2821 aaactgtaaa ccagacgctc aaacgtttag tcgtagattt agcaaaccat cccttcctcc
     2881 ttttcctagc ctttctagga actattgccc aagttggctt atcaatttac ctacctattc
     2941 tgattgggca ggtcattgac caagtcctag tggctggttc atcaccagtt ttttggcaga
     3001 tttttctcca gatgctcttg gtggtaatag gaaatactct ggtacaatgg gccaatcctc
     3061 tcctctataa tcgtctaatc ttctcttata ccagagattt acgagagcga atcatccata
     3121 aactccatcg gttaccgatt gcttttgcgg atcggcaagg caatggagag atggtcagtc
     3181 gtgtaaccac ggatatcgaa cagttggcag ctggcttgac catgattttt aaccaatttt
     3241 tcattggtgt tttgatgatt ttggttagta ttctagccat gctccaaatt catctcctca
     3301 tgactctctt agtcttgctg ttgacgccac tgtccatggt gatttcacgc tttattgcca
     3361 agagatccta tcatctcttc cagaagcaaa cagagacgag gggaattcag actcagttga
     3421 ttgaagaatc gcttagccag cagactataa tccagtcctt caatgctcaa acagaattta
     3481 tccaaagatt gcgtgaggct catgacaact actcaggcta ttctcagtca gccatctttt
     3541 attcttcaac ggtcaatcct tcgactcgct ttgtaaatgc actcatttat gcccttttag
     3601 ctggagtagg agcttatcgt atcatgatgg gttcaacctt gaccgtcggt cgtttagtga
     3661 cttttttgaa ctatgttcag caatacacca agccctttaa cgatatttct tcagtgctag
     3721 ctgagttgca aagtgctctg gcttgcgtag agcgtatcta tggggtctta gatagccctg
     3781 aagtggctga aacaggtaag gaagtcttga ccagtgacca agttaaggga gctatttcct
     3841 ttaaacatgt ctcttttggc taccatcctg aaaaaatttt gattaaggag ttgtctatcg
     3901 atattccagc tggtagtaag gtagccatcg ttggtccgac aggtgctgga aaatcaactc
     3961 ttatcaatct ccttatgcgt ttttatccca ttagctcggg agatatcttg ctggatgggc
     4021 aatccattta tgattataca cgagtatcat tgagacagca gtttggtatg gtgcttcaag
     4081 aaacctggct cacacaaggg accattcatg ataatattgc ctttggcaat cctgaagtca
     4141 gtcgagagca agtaattgct gctgccaaag cagctaatgc agactttttc atccaacagt
     4201 tgccacaggg atacgatacc aagttggaaa atgctggaga atctctctct gtcggccaag
     4261 ctcagctctt gaccatagcc cgagtctttc tggctattcc aaagattctt atcttagacg
     4321 aggcaacttc ttccattgat acacggacag aagtgctggt acaggatgcc tttgcaaaac
     4381 tcatgaaggg ccgcacaagc ttcatcattg ctcaccgttt gtcaaccatt caggatgcgg
     4441 atttaattct tgtcttagta gatggtgata ttgttgaata tggtaaccat caagaactca
     4501 tggatagaaa gggtaagtat taccaaatgc aaaaagctgc ggcttttagt tctgaataag
     4561 ccattctctt ttgaaagttt atggacgaaa aaagttgcct tcgagtgact tttttgttac
     4621 aatagctaga aaaattgttc actgtaatac tcaatgaaaa ttaaagagca aactaggaag
     4681 ctagccgtag gttgctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagt
     4741 aaccacacct acggcaaagt gaatctgaag tggtttgaag agagtacaac ttgtctttta
     4801 gaaaaggagc ctataatgaa agtctttcag catgtaaata tcgtgacttg tgatcaagat
     4861 ttccatgttt atcttgatgg aatcttagca gtcaaggatt ctcaaatcgt ctatgtcggt
     4921 caagataagc cagcgttttt agagcaagct gagcagatta tagactatca gggagcttgg
     4981 attatgcctg gtttggtcaa ttgtcacacc cattctgcaa tgacaggtct gagagggatc
     5041 cgagatgaca gcaatctcca tgaatggctc aatgactata tctggccagc agaatctgag
     5101 tttactcccg acatgactac caatgcggtc aaagaagccc taacagagat gctccagtca
     5161 ggaacaacaa cctttaacga tatgtataat cccaatggtg tggatatcca gcaaatttat
     5221 caggtggtga aaacttccaa gatgcgttgt tatttttctc cgactctctt ttcttcagag
     5281 acagcaacaa ctgctgagac tataagcaga actcgatcca tcatagatga aatcttaaaa
     5341 tataaaaatc caaatttcaa ggttatggta gcacctcatt ctccgtatag ctgcagtaga
     5401 gacttgctgg aagcgagttt ggaaatggca aaagagctaa atattccgct ccatgtccat
     5461 gtggcggaga ccaaggagga gtcaggaatt atcctcaaac gatacggcaa acgccccctt
     5521 gcttttcttg aagaactggg ttatttagat catccgtccg tatttgctca cggggtcgaa
     5581 ttaaacgaga gagaaattga acgcttggca tcttctcaag tggctatcgc ccacaatcct
     5641 atcagtaacc tcaaactggc atcaggaatt gctccaatta tccagctcca aaaagcggga
     5701 gtagcagtcg gaattgcgac tgactcggtt gcttccaata acaatctaga tatgtttgag
     5761 gaaggaagga ctgcagctct tcttcagaag atgaaaagtg gggatgccag ccagtttcca
     5821 atcgaaacag ctctcaaggt actgacaatc gaaggggcta aggtccttgg aatggaaaat
     5881 cagataggaa gtctggaagt cggcaagcaa gcagattttc tggtcattca accacaaggg
     5941 aaaattcatc tccaacctca ggaaaatatg ctgtctcacc tggtttatgc agttaaatct
     6001 agtgatgtag atgatgttta tatcgccgga gaacaggttg ttaagcaagg tcaagtcctg
     6061 acagtagaac tttaaaagaa aaatcacgaa aaattttaaa aaaagtttgc aaaaatcttg
     6121 cattcttttt ttgactatgc tatacttata tacggtttaa aaaaactgcc taagacagta
     6181 ggggagctcg actcataaat atcctaccga ggacaaaacg tatcatgtaa aaagaagcgt
     6241 attgtacttt cgtgtctagg tttgggcgcg tttttctttt tgaaaaattc cccaagcaaa
     6301 ataattacgg aggtgaacac actaatgagt gaagcaatta ttgctaaaaa agcggaacta
     6361 gttgacgtag tagctgaaaa aatgaaagct gctgcatcta tcgtcgttgt agacgctcgt
     6421 ggtttgacag ttgagcaaga tacagttctt cgtcgtgagc ttcgtggaag cgaagttgag
     6481 tataaagtta ttaaaaactc aatcttgcgt cgtgcagctg aaaaagctgg tcttgaagat
     6541 cttgcatctg tatttgttgg accatctgca gtagcatttt ctaatgaaga tgttatcgca
     6601 ccagcgaaaa tcttgaacga cttttctaaa aacgctgaag cacttgaaat taaaggtggt
     6661 gcaatcgaag gcgctgtcgc atctaaagaa gagattcttg cacttgcaac tcttccaaac
     6721 cgcgaaggac ttctttctat gctcctttct gtacttcaag cgccagtgcg caacgttgct
     6781 cttgcagtca aagcggttgc agaaagcaaa gaagacgcgg cttaatctta agctacacag
     6841 cgtagcctag ctacgaaaaa aactattata aaatttaaaa cttatttgga ggaaataaca
     6901 atggcattga acattgaaaa cattattgct gaaattaaag aagcttcaat ccttgaattg
     6961 aacgaccttg taaaagctat cgaagaagaa tttggtgtaa ctgcagctgc tcctgtagct
     7021 gttgctgcag ctgatgcagc tgatgctggt gctgctaaag attcattcga cgttgaattg
     7081 acatctgcag gcgacaaaaa agttggcgtt atcaaagttg tacgtgaaat tactggtctt
     7141 ggtcttaaag aagctaaaga acttgttgac ggtgcaccag cacttgttaa agaaggcgtt
     7201 gcaactgcag aagctgaaga aatcaaagct aaattggaag aagctggagc ttcagttact
     7261 cttaaataat agagtatcgc aatttaattg cggaatccct atttaccagt ctttgagagc
     7321 gataagcgat tgatagaaac tgataaattg atttggtttc ctgccaaaaa ttctttggca
     7381 ggatttttgt tttaataaac tttcgggggt agacactaga ttgtctaccc ctttttttgt
     7441 gtttcggagg taattatgca aaagaaagga cagtcatcac gtcaattcgt gaagagtcat
     7501 ctcatgacta tcatgggagt tgatattgta aaagcaactc agttagttga tgaaatggaa
     7561 caagtaggtt taatccgatt tgatgaattt ggaaatgttg aaattttagt cttggaggga
     7621 caatcatgaa gcgtattacc gctagtcaat atcagacatc agagcgttat tataagctcc
     7681 ccaaactttt atttgagagt gaacggtata agaatatgaa actagaagta aaggtggctt
     7741 attctatttt aaaagatcgt ttagaattat ctctcaatag tggttggata gatgaagaag
     7801 gggcggtcta tttagtattt tctaactcta aactgatgaa gcttttaggc tgttcaaagt
     7861 caaaattact ctccatcaag aaaactttac gtgagtatgg cctaattgat gaagtccaac
     7921 agtcttccag tgaaaaaggt cgtatggcta ataaaattta cttaggggaa ttagaacatg
     7981 agatacccct agtcttacat tcagacgggg ctagtgttaa aaacagatta gggcagtctc
     8041 aaaaacagac ggggccgttt ttaaattcag cccctagtga gactgaagaa agtgagactg
     8101 attctttatt tattgaggat gaggaggaga gggatactca acctatcttg aaaagaaaag
     8161 tagaaaaggt cacaaaatat gatcgagatt atatttgggg attggtacaa gatcaatttt
     8221 gacgagaggg
//
LOCUS       DAVBDG010000075         7965 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBDG010000075 DAVBDG010000000
VERSION     DAVBDG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.076"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            25..339
                     /locus_tag="VQ526_001802"
                     /pseudo
     CDS             25..339
                     /locus_tag="VQ526_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            516..1175
                     /locus_tag="VQ526_001803"
     CDS             516..1175
                     /locus_tag="VQ526_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2555499.1"
                     /translation="MKNLTKIKFKENGEFNHFSGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1200..2267
                     /locus_tag="VQ526_001804"
     CDS             1200..2267
                     /locus_tag="VQ526_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2555500.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            2370..3380
                     /locus_tag="VQ526_001805"
     CDS             2370..3380
                     /locus_tag="VQ526_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555501.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3392..5083
                     /locus_tag="VQ526_001806"
     CDS             3392..5083
                     /locus_tag="VQ526_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2555502.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5086..5796
                     /locus_tag="VQ526_001807"
     CDS             5086..5796
                     /locus_tag="VQ526_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2555503.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            5892..6083
                     /locus_tag="VQ526_001808"
     CDS             5892..6083
                     /locus_tag="VQ526_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555504.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKC"
     gene            6368..7369
                     /locus_tag="VQ526_001809"
     CDS             6368..7369
                     /locus_tag="VQ526_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2555505.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         2669 a         1076 c         1515 g         2705 t
ORIGIN      
        1 aaaaagggaa aaatgagata aaagatccat gtagtagatt tagcaatagg acatataaaa
       61 gctttagaaa aagtatctga aaaaacagat gtttatatct ataacctagg ctcgggagaa
      121 ggcactagtg tattacaact tgtaaataca tttgaaagtg ttaataagat ccctattcct
      181 tataaaatag ttccaagacg ttcaggagac gttgcaattt gttatgcaaa tgcagacaaa
      241 gcatataagg aattaaattg gaggacaaca aaatcgattg aagacatgtg tagagataca
      301 tggaattggc aatcaaaaaa tcccaatgac tataattgat gatactgtct atatgagtgc
      361 tgtattttgg aaaaattact taacctttta atgaaatgca tatttaatga aagataaata
      421 aaaaaagttt gacagcgctt gacaaaggaa acggtttcat tgtataatgt aaacatggtg
      481 acaccgatgt caaatataaa aaagaaggtc tttatatgaa aaacttaaca aaaattaagt
      541 tcaaagagaa tggggaattt aatcatttct ctgggaatac agttgtagca aatctttata
      601 ctaaacaaga tttgatggaa gttgttgata ttattcaatc acgttataga gaattaccat
      661 ttatcgataa gtttacttta actccaagga attctattca catgactgta attgaattgt
      721 tgtgccatga aaatcgtgaa acggagtttt ggagcagtaa tcttcctcta gatacacctt
      781 tacaggaaat acatgattac tttgctaaac aacttgaaat ttttccattg ttggatgaag
      841 aaattcatat gcgtataact gaaatgggaa aacaaaacat actagttgaa cctgcagatg
      901 aagcttctgc aaagagatta gaagaaattc gtacttatgt ctcagaaaaa gcaggtgttt
      961 gtttccctaa tcatgataga tatcaattcc atatttcaat tgggtatctt cggattcctc
     1021 taaccgaaga ggaggaagaa gagtttacta aagtcagagc agaattaact gaaattttat
     1081 tagagaagat tccaactatt actgtaaacc gtattgacta tactgtattt gaagatatga
     1141 gacaatttgt tccatatcac gaaaaattta aataaaataa taaaaaagga gaacttatca
     1201 tgaaaaaaat gaaagtttgg tctactgtac ttgcaacggg agttgctctt actacacttg
     1261 ctgcttgctc tggaggttca aattctacga ctgcttcttc atctgaagaa aaagctgata
     1321 aaagtcaaga attagtaatc tattcgaact cagtctcaaa tggtcgtggt gattggttaa
     1381 ctgctaaagc aaaagaagct ggttttaata taaaaatggt tgatatcgct ggcgctcaat
     1441 tagcagaccg tgttattgct gagaagaata atgcagttgc agatatggta tttggaattg
     1501 gtgctgttga ctcaaataaa attagagatc aaaaattact agtacagtac aagcctaaat
     1561 ggttagataa aattgatcaa tctttatcag ataaagataa ttattataat cctgtgattg
     1621 ttcaaccatt agttttaatt ggggcgcctg atgtaaaaga aatgcctaaa gattggactg
     1681 aattaggtag taagtataaa ggtaaatatt caatttatgg tcttcaaggt ggtacaggac
     1741 gggcaattct agcaagtatc ttagttcgtt accttgatga aaaaggagat ttaggcgtat
     1801 cagaaaaagg atgggaagtt gcaaaagaat actttgcaaa tgcctatact cttcaaaaag
     1861 gtgaaagctc agttgttaaa gcgttagata aagaaagccc aattcaatac ggtatgatct
     1921 ggggttctgg agcgttaatc ggacaaaaag aacaaaatgt tgaattcaaa gttatgactc
     1981 ctgagattgg tgtaccattt gtaactgaac aaactatggt tttaagcact agtaaaaaac
     2041 aagcgttagc taaagaattt attgattggt ttggtcaatc agaaattcaa gtagaatata
     2101 gtaaaaaatt tggctctatc cctgccaata aagatgccct aaaagagtta cctgaagata
     2161 cgaaaaaatt tgttgatcaa gtgaaaccac aaaatattga ctgggaagct gttggaaagc
     2221 atttggatga atgggtagaa aaagctgaat tagaatacgt acaataagaa ataagagtta
     2281 ttttttatgt ccaagtctct ctattatttc taaatttatg gaagaaataa agagaggctt
     2341 ttggtttagt aatagaggga gttagtgaga tgattaaatt tgataatatt caaattaaat
     2401 atggtgattt tgttgcaatt gataatctga atttagatat acatgaaggg gaattcttta
     2461 catttcttgg gccttcagga tgtggtaaat caactacttt gagagcattg gtaggttttt
     2521 tagatccatc atcaggaagt attgaagtta atggaacaga tgtcactcat ttggaacctg
     2581 aaaagcgtgg aattggtatt gtatttcaat cttatgcgct atttccaact atgactgttt
     2641 ttgataatat tgcatttggt ttaaaagtta agaaggtagc tccagatgtt attaaagcta
     2701 aagtatcagc agtggcagca aaaattaaga tctctgatca acagttacag cgtaatgtat
     2761 cagaattatc tgggggtcaa caacaaaggg tagcattggc tcgtgctctg gttcttgaac
     2821 ctaaaattct ttgtctagat gaaccattgt caaaccttga cgcaaaatta cgtgtagatt
     2881 tgagaaaaga gttgaaaaga cttcaaaaag agttaggtat tactacttta tatgttactc
     2941 atgatcaaga ggaagccttg actttatctg atagaattgc agtctttaac aatggataca
     3001 tcgaacaggt cggtacacca gtagagattt atcataattc tcaaactgaa tttgtatgtg
     3061 attttattgg agatattaat gttttgaccg atgaaacagt ccacgaagta ttactgaaaa
     3121 atacaagcgt tttcttagaa gataaaaaag gatacattcg attagagaaa gttcgattca
     3181 atcgtgaaac tgaacaagat ttcattctaa aagggacaat tattgatgtt gagttttctg
     3241 gagttacaat tcactataca ataaaagttt ctgaaagtca gattcttaat gtaacaagta
     3301 ttgatagtca ggctgctatt agatctgtcg gagaaagtgt ggaattattt atcacaccat
     3361 cagacgttct gcaattttaa ggaggtgcag tatgcgtcat aaattaaatt taaaagattg
     3421 gcttattcgt ttagggttaa tctggttctt agtaacattt attatttatc caaactttga
     3481 tctagtagtg aatgtatttg taaaaggagg agaattttcc cttgatgctg tacatcgtgt
     3541 tctaaaatct cagagggcac ttcagagtat tatgaacagt tttaagttag cattttcact
     3601 cattattaca gttaatgtcg taggtattct ttgtgttcta tttacagagt actttgatat
     3661 taaaggtgct aaaattttaa aattaggtta tatgacctct ttaatttatg gaggagtggt
     3721 tttagcgact ggatataaat ttgtctatgg tccttatgga ttgattacaa aatttttaca
     3781 aaatgttatc ccttctttag actctaactg gtttattggg tatggtgcag tcttattcat
     3841 tatgacattt tcaggaactg ctaatcatac attgttttta acaaatacaa ttcgaagcgt
     3901 tgactatcac actattgagg ctgctcgaaa tatgggagca aaaccattta ctgttttccg
     3961 aaaagtagtg ttaccaacct taattccaac tctatttgca cttactatta tggtttttct
     4021 tagtggttta tctgcagtag cagcacccat gattgttggt ggtaaagaat ttcaaactat
     4081 aaatccaatg attattacat ttgcagggat ggggaattct cgtgatttag ctgccttact
     4141 tgcaattatt ttaggtattg caactacaat tttgcttact atcatgaata agatagaaaa
     4201 aggtggaaat tatatttcta tctctaagac taaagcgcct cttaaaaaac aaaaaattgc
     4261 gtctaagcct tggaatatca ttgctcacat tgtagcatat ggattgttca cagttttcat
     4321 gcttccacta atttttatag tattatactc atttacagat ccagttgcaa ttcaaacagg
     4381 taacttaaca ttatcaaact ttactttaga aaattatcgc ttattcttta gtaatagtgc
     4441 ggcattctct ccattcttgg tcagctttat ttattctatt attgctgcga caacagcaac
     4501 aattctcgca gttgtatttg ctcgtgttgt cagaaaacat aaatctcgtt ttgatttctt
     4561 atttgaatat ggtgctctac ttccttggtt actaccaagt acacttttag cagtaagttt
     4621 attatttact tttaatcagc cacaatttct tgtcttgaat cagattttgg taggtagttt
     4681 ggtaattcta cttattgcat atatagttgt aaaaatccca ttttcttata gaatggtacg
     4741 tgctatttta tttagtgttg atgatgagat ggaagatgca gcaagaagta tgggtgcttc
     4801 acctttttat actatgatga aggttatcat tccatttatt ttaccggttg ttctctctgt
     4861 tattgcttta aactttaact ctttattaac tgacttcgac ttatctgtat tcctttacca
     4921 tcccctagct caaccattag gtattacgat tcgatctgca ggtgatgaaa cagcaacatc
     4981 taatgcacaa gctctggtat ttgtttatac aattgttctg atgattattt ctggaacggt
     5041 attatacttc acacaaagac cggggcgtaa agtaaggaaa taatcatgac agccactagt
     5101 cttgggttat caaatattga aatagttgtc aggattgttt tatcagtagt cattggtagt
     5161 ataattggtt tagagagagg gagcaaatcc cagcctgcag gcatccgaac ttatagtatt
     5221 gtttgtctag ctgcatgttt gattatgatg acgaatgaat acgtatctta taaatttggg
     5281 acaggagatc ctacacgatt aggagctcaa gttatatcag gtgtgggttt tctaggcgct
     5341 ggaacgattc ttattacaga taaaaagaaa attacaggtc tgacaactgc agcaggcatt
     5401 tgggcttcgg caggaattgg attagctatt ggagtaggtt tttatgaggg agctctttta
     5461 gtagctattt ctgtttgggg tgtgatatcc atgttccaac cactaaaaaa atatctgcaa
     5521 aatcgttcta aaatgattga attgtatata gtggttaaat ctacagaagc ctataatcgt
     5581 gtattggttt attgtgcaga aaatggtatc agaatgaccg attcaagaac agcgtttgga
     5641 gatgttaatt ctgacagaat tgaatacttt gatgttccag ataaaaaaat agcgtcattt
     5701 attactctgg aattatcagg tagatttgaa catcttcgac tgatggaaga aattgctaat
     5761 attgtaggtg ttatttatgt cgaagaaatt agctgaaatt tagtaaacaa agggacagat
     5821 gtttttgtcc ttttgttttt tagatgtttt aatcatctaa tttttgagta gatttttgct
     5881 cagaggtttt tatgaaagat tataaaatca attttgattt aggtaaaata gaatattttg
     5941 ataataattg tttgatacaa gtatacaaat ttatttcttt ttatgatatt tgtgaaatgg
     6001 tgtttgcctt tcatttacct cctgatgagt tgataacaaa tgttatcttt aaagaaaaaa
     6061 ttaactctat gttaaaatgt taaattgaca ggctattata tgtttttata aatcctacac
     6121 attttactga aaaagttaat ctacaatttt atggtagttt tttctcatat gaatttattt
     6181 gtcgtgaagt tggaaacatt cttaaaaata aaggtgtgaa atgcaattta aacttttttg
     6241 aagggaaaga atatttgtaa aattaatttt gtgaatttgt ggatcttaaa taatttacta
     6301 aattctcttg ttaaaaaaaa ttaaatattg tatagtaata ttagaatgtt cgtaatggag
     6361 gactgaaatg aaaaatataa atgggaaaaa ggtaactatt tatgatattg cacgtttatc
     6421 aggtttttct ccaaaaacag tttcacgagt tattaatggt ggagtaaatg ttaaagaaga
     6481 aacttatcaa gcgattcaaa aagttataga ggaattgtcg tatattccaa atgcgtatgc
     6541 aaaaaattta actaaaaaag aagctataaa tatattgatt tctgtaaaaa aaatcgactc
     6601 ttttccttta atttggtttc atacgctgtt agataaagtg ttacggacgt gtaaagaatt
     6661 tggtgttaat gctatagttg agtactttgg agaggaggat acaattagta attcaattat
     6721 atcaagcaca ggtagcctag tagatggtgt tattgttttt tatgaaagtg tagatgatat
     6781 cagaattcag tatttaaaga agaaccatat gccttttctt gttttcggtg aatctcaaac
     6841 gtctggagta gtctatgtat ctaataataa ttttcaagct acttatgata tgatgaaagc
     6901 tgtaactgaa gaaaaattta aaaatatgtg gttgcttatg ggaggagaat cccatgttaa
     6961 taaggatcgt gaaagaggtg tgcgttcttt tctgaatgat aaaaattact ttatggattt
     7021 aaaagtcatt tatggcttat ctacaattga ttcagtttat tcttatgcaa tgcagcactt
     7081 gactacccaa aattatccgg acattatttt tgtttcagga gatgaaaagg ttcaaggctt
     7141 gattcgtgcg tgctatgaga aaggaattct tattccagat gatatttcta tcattggatt
     7201 tgataatatt cctatttctc agtattatac tccagctttg tctacaattg ctcccaatta
     7261 tgttaaattg gctaaagaaa tgatagaagg tgttttagca attattaaag gggaaagtgt
     7321 cacatctgtt gaagtttctc ctaaatttgt taggagacag agtttctagt attgtttaat
     7381 tcttgaaaat cctagtggaa aagatggagt gttctaaaat aagttctgtt tagcctccat
     7441 tcagtttcta ttgagttgga tccaatttct aacgagtggg acatgttcat gttcgatcta
     7501 aggaagacat ggtcacattc gaatgatatt ttcaaaaaaa tcgtaaaaaa gtcttgacaa
     7561 agaaaaaaca aagtattaaa ataagttcaa caaatcagaa aagtaactat ttttgatctt
     7621 cagggagcct gtggtgattg tgaataggtg gttggaagta gtgaaagtgg gctgattttg
     7681 aaaatgaatt tgaaacaatg aaaattcggt gcgcacacct tacagtgcag cttgttgtta
     7741 aacaaggcag agatgtaaag ggatagtccc tttataattg aggtggcacc gcgttaccaa
     7801 cgccctcaca cggaagtata ttctgtgtgt gggctttttt ctatccgtcg tttgctttat
     7861 cttttattag ggccttaact cgctttgatg aacttgagtt ctatctacag ctccttgcct
     7921 cctactaaaa gtaaacaaaa aggctggact aatacgaaaa aagct
//
LOCUS       DAVBDG010000076         7763 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBDG010000076 DAVBDG010000000
VERSION     DAVBDG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.077"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(70..903)
                     /locus_tag="VQ526_001810"
     CDS             complement(70..903)
                     /locus_tag="VQ526_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2555506.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(917..1783)
                     /locus_tag="VQ526_001811"
     CDS             complement(917..1783)
                     /locus_tag="VQ526_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2555507.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIIAIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFFQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(1797..3056)
                     /locus_tag="VQ526_001812"
     CDS             complement(1797..3056)
                     /locus_tag="VQ526_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2555508.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(3148..5310)
                     /locus_tag="VQ526_001813"
     CDS             complement(3148..5310)
                     /locus_tag="VQ526_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2555509.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLFYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            5418..6278
                     /locus_tag="VQ526_001814"
     CDS             5418..6278
                     /locus_tag="VQ526_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2555510.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            6275..7210
                     /locus_tag="VQ526_001815"
     CDS             6275..7210
                     /locus_tag="VQ526_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2555511.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(7336..7423)
                     /locus_tag="VQ526_001816"
     tRNA            complement(7336..7423)
                     /locus_tag="VQ526_001816"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7387..7389),aa:Ser,seq:gct)
     gene            complement(7430..7503)
                     /locus_tag="VQ526_001817"
     tRNA            complement(7430..7503)
                     /locus_tag="VQ526_001817"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7467..7469),aa:Ile,seq:gat)
     gene            complement(7539..7609)
                     /locus_tag="VQ526_001818"
     tRNA            complement(7539..7609)
                     /locus_tag="VQ526_001818"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7575..7577),aa:Gly,seq:tcc)
     gene            complement(7629..7701)
                     /locus_tag="VQ526_001819"
     tRNA            complement(7629..7701)
                     /locus_tag="VQ526_001819"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7666..7668),aa:Phe,seq:gaa)
BASE COUNT         2458 a         1656 c         1418 g         2231 t
ORIGIN      
        1 aacctcaaaa cagtgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
       61 cattgagtat tacttcactg ccccgttgct cattcctgaa atgatatggc gttggaagaa
      121 gagatagaca atggtgatac tgataatgcc gaccacgtaa gaggcaaagc ttggtccgta
      181 gtcgttgaaa tattggcctg cgtagttgta ttggaacaaa ggcagagtcc acattttgga
      241 atcccggttc aagacaagga gtggcaacat gaagtcattc cagaaccaaa gggcattgat
      301 gatcatggtt gtcgcatgca tcggtttcat cattgggaag atgatgcgga aataggttgt
      361 aaattgatta gccccatcga tctctgctgc ttcatccaga ctttctggaa tcgagatttt
      421 gatatagcca acatagagaa agagggtctg tggaatcgca taggtcaagt agagcaagat
      481 caaaccaaag gtattagcca aaccgagttt actcatcata accgtaatcg gaatcatgat
      541 gacttggaaa ggtacgaaga ttccgaggat taagagggta tacatgatgg taaaggcttt
      601 tcttttactc atattgcgag cgatggagta ggctgccata gggataaaga tcattactgc
      661 aagtaaagac aagacagtga tgacgacaga gttccaataa tagcctccaa tcccatcagc
      721 taagagacgg ctaaagttgt cccatgtgaa gttggttgga aagccaaaga aattatctac
      781 aatatcctta gtgggtttga aggaactaaa gagggtagca aggagcggca ctaaaatcag
      841 aaccgatcct agaatcaata gaatgtattt gccaatcagg gcttttcttt catcttgttt
      901 catcatgctt ctcctcttaa atttcaaatt tcttagatac tctcaattgg atgatcgaaa
      961 tcactacaat taagaagaac aagattacgg caatggcatt ggcataaccg aattggttgt
     1021 ttttaaaggc atagttataa accaagagcc caagtgaggt tgtggcattg tttggaccac
     1081 caccggtcat ggcaaagact tggtcaaagg cagtcagccc accttttagg gctaggataa
     1141 agaccataga gacacttggt agcaagtaag gcaattcaat gttccagaaa acttgcttgc
     1201 tagtcgcacc atcaatcctt gctgcctctg taatctcagt tggaatagat tgcaaaccag
     1261 ctaggaagat gatgatgggc atagccaccc cttgccaaag aaggacaaag acagccgcaa
     1321 agattgctcc ccacttagtc cctaaaagac tggtttggaa aaattcaata tgaagggcat
     1381 ttccaatcgc tggaagaccg tagttgaaga cttgcttgaa gatcaaagcc actgtcaaac
     1441 cagataaaac agctgggaag aagaaccaag cacggaagaa ggtttggcct ttgattttag
     1501 aattcaagac acgcgcaatg aagatcccga gtgcaatctc accaaccacc atggcaatcg
     1561 caatgattgc ggtaaagcca atcgcattca tgaattttgg atccatgaag aggagcttaa
     1621 agttgtttaa gccaacaaat ttgtagttat aagtcaatcc tgtccagttg gtaaaactgt
     1681 aaaaggctcc ttgaaacatc ggcacataga agaaaattgc ttgtaacaag agggggatga
     1741 ccacaaaagc ccatgcccaa tatttttgta atactttttt catagtctct ctactcctaa
     1801 tccacatccg ctttcatcgg gttaaagaag gcattcaaat cattgaccat gccttgttta
     1861 tcaccggtca agacatagtt catggtcaag gtatggaagt ctgcttcact ggtccagtat
     1921 tgttgcaacc agaccaagtg acgatccgta aaggcatatt cggtcatacc agcaagcggt
     1981 gaatcttctc ctgcttgttt gaccccttcg atcgctgttg gagatccgtc cacatcgtag
     2041 tatttttgca tgacttctgg acgggtcata tattccacaa aggcattggc ttcttttgga
     2101 tgtttggtgg tggctgagat agaccatgcc aagtctcccg caccaacggt taagctttgt
     2161 cctttttctt ttcctggaat catgaaggtc ccaatcttaa agttcggttt ttgttcatta
     2221 atcgctgtga tcgcccaaga cccatttggt gtcatgagga catccccacg tgcgaaggct
     2281 ccgataacat cggtatagcc agcaccttcc cagttctttt gcttagagcc attgatgcga
     2341 aggatgtcca tgaccttgat atcatctttc ataatcggat ccgacaattt aatggcattt
     2401 ggttgagaat aacgaaggta ttgatttgct tcttttcctc cacctgttgc tgtcgcaaag
     2461 gctaattgat tgtaaccatt gagtgtccaa gcatctgcac ctgcaattcc aaatggtgtt
     2521 tgtcctttag caacgatatc tttgactaac tgttcaaatt catcccaggt ttcaggaacc
     2581 ttcaagccca gttcttcgaa tttatctttg ttgtagtaaa ttccataagc attagctgta
     2641 aaaggaacgt tgtaaacttt ttcgtttaca gcatattttt cagcgtagcc atttttcacg
     2701 cgtttcaggt agtctttgtt gctcaaatct tcaaaaacac ctgcttttgc ccattcttgc
     2761 agttcgatgg actgtgggta aatattgacc acatcaggca catctcctgc gagaacgcgt
     2821 gtcttcaata cttcaccagc atttggtaca ttgacgactt tgaccttgat cttagggttt
     2881 tccttctcaa aatcacgagt gatttcttcc aaggttttgg tcatttcttt tttctggttg
     2941 aaatactcga tggtcactgt gccatccgca gatttaccat agttggagca agcgccgagc
     3001 ccaaacaaag ctaaacctgt agttgcaaga agtccgattt ttttatacca ttccattaga
     3061 aagcctcctt tataaattta tacaccctta ttgaactgca ccccaaaagt tagacagaat
     3121 aaatctaact tttggggtca gtacatatca tagttttcta aaaatatact gtctactcaa
     3181 aaaatctcct tgggataaga taacagttaa gcccgcatac attagttctg cacctgagta
     3241 aacttcgcca ttttcctgta atttatatag tccctcttca tccaaatctt ttaattttaa
     3301 agttgtttcc atggtctcta caacagataa aacgcgaacg taggttacaa tcgtttgatt
     3361 tccgtaatta aattgtacag ctgcttcatt ggatacagta tcaggattaa ttagtctata
     3421 ctgctgtcct aactgaacta ctggtcgtaa ttctttatac aagttcacct gattagcaat
     3481 cgtagctttc tcttcatctg ataaatttgt caaatcaagt tcatagccca aatttcccat
     3541 cattgctaca aggccacgtg tttctaatgg tgtcattcgt cccatctgat gattcggtac
     3601 tgctgacaca tgagccccca tagaaatggt tggatagaga taggatgaac cgtattgaat
     3661 tggtaaacgt gcaatggcat cagtattatc actagcccag acttgtggga aatagcgcat
     3721 cataccaaga tcatttcgtc caccaccacc agagcaggac tcaaagagaa tatggctgtg
     3781 cttctctgtc agataagaaa cgagttcata aagccccagc atgtactgat gagattgcat
     3841 ctgtgtctct agataagtta atccattccc tagcttagtg atattgcggt tcatatccca
     3901 tttaatgtaa tcaatatcat gataaaatag gagttgatct aagacacttt tcaagtattc
     3961 tactacctga ggattggcaa gattaagtac taattgattc cgagaataag tatgctcata
     4021 gccaggaacc tgaatagccc agtcaggatg ttgacgatac aaatcactat ctacagaaat
     4081 catttcgggt tctaaccaaa gtccaaactg caaacctctt tcatggatag ctgaaatcag
     4141 actttctaga cttccaccca gtttttcctc attaacaacc caatcaccta aagcacgatt
     4201 atcatcaaaa cgattgccaa accaaccatc atctaataca aaaagttcaa tgccaacttt
     4261 cttagcttca tctgctaact ctaacagttt ttctctctga aagtcaaagt aagtagcttc
     4321 ccagttattg attagaattg gacgttcttt tttagaaaat tcacttagca taatgtgctt
     4381 cagtacaaaa ttctgacttt catgactaat accagttaat ccctgatctg aatgagtcac
     4441 taaagctacc ggtgtttcaa agtattcctc aggagctaac ttccaagaaa agttttctgg
     4501 attaatgcca atagccaccc gaacttcatt caattgattt ttttgaacaa aagcttcaaa
     4561 gttgccacta tacattagtt gaatagcaaa cacattccca gcatcctctg tgactccttg
     4621 ttcgcatagt agaagagctg gtgtttgagc atgaccagaa gcacctcggt ttgaactaat
     4681 cgaaaagatt ccttgttcta cctgttgacg tctaacagtc ttttcacgag cataagcacc
     4741 ctgcagagtt actatttcgt aatctgcagc tggaaaatca gccataaaag aaaaatcttt
     4801 atggatgaca acttcctgat tactattatt atctaattta ctgtagctag caatagtcgc
     4861 atcattatta aaagtagtat aatacaaagt cagactaagt tgagccttag aatcttctaa
     4921 cattaagaca agagtctctg tatcgtccat gctatgtgga gaaggtaagc cctgtggacc
     4981 attctgacct tttaaaatct ttgcttctac aaatcgaaag tctgttactt cagttacact
     5041 atgctgaacc tgtatggttg gtttcctaaa atctcctaag ccatgttgtc caaaaatctg
     5101 tcgctgagta tctaaactaa aggttcgatt agtagccgtt ggatttcctg aaaaggcatg
     5161 gtctcgttca taaacactat tggaaccttt atagttctta atagtctttc ctaaatgttt
     5221 caaaagtaag tagccatttc gattttcaat aatcaaactt agatttttac tctcaacata
     5281 aaatagatta ttctctatcc taactcccat ttacttcacc tcatcacttt attgattata
     5341 ttttatcacc tgaaatcgct ttccaaaata gaaaaatgtc tcaagaatat ggtaaaatgt
     5401 taggtaggag gtagcacatg ttagtttttt cagaatacca gactggaaca atcgaccttg
     5461 ccctaagctt ttatggatat gaggaatgca cacctaatta ctcttttggt ccagccattc
     5521 gtgatacata cgttctacat tacattacta aaggacaagg aaaatttcat tacaagggta
     5581 aaattgttga tttaaaagaa ggagatttct ttctattaaa accagaggaa ctaacctttt
     5641 atcaagcaga tagtaaagaa ccttgggcct actactggtt aggaatcact ggagggaaag
     5701 cccctgatta ttttgctctt tcccaaattt ctgatcaatc ctatctcatc caatctgaaa
     5761 cttgtcatac ccagactact gcaaaactca tctcagacat tgtccgcttc gctcagatta
     5821 caaaatcaag tgaattagct caactccata tcatgggaca acttcatgaa ctgatgtttc
     5881 atctgggaac tattgctccc aatcagaaaa aaaagaatat ttcatcaacc caccaactct
     5941 atcttgaatg caaacgatta attgatagcc actatcctca atcacttaca attcaagatt
     6001 tagcaaaaga actatccgtt cacagaagct acttatcaag cgtattcaaa gaatttaata
     6061 ccttatcacc caaagaatac ctactctacg ttcgaatgca ccgagctaga caacttctcg
     6121 aaaataccca agagtccatc aaggtaattg catactcggt aggtttttca gatccactcc
     6181 atttttcgaa agcttataaa caatacttta atcagactcc aagtcataca agaaaagaat
     6241 actctcaata ccaactagta agaaaggcaa cattatgaaa tcctaccaag ctgtctacca
     6301 aatcctatct aaagaaaccg actatatcag cggagaaaaa atcgcagaaa aactatccct
     6361 aagccgaaca gcaatttgga aagccatcaa gcgactagaa caagaaggca ttgaaattga
     6421 tagtatcaaa aatagaggat ataaactgat gaatggtgac cttattcttc cagagattct
     6481 agaagaaaat cttccaatta aagtcagctt taaacccgaa acaaaatcaa cacaactaga
     6541 tgcaaaagaa gcaattgatt taggccatga agcaaatacc ctctatctag cttcctatca
     6601 aacagcaggc cgaggccgtt ttcaacgttc cttctactca ccacaaggtg gtatttatat
     6661 gacactccat cttaaaccaa atctccccta tgacaaatta ccatcctaca cactacttgt
     6721 agctggagct gtctacaaag ccattaagaa cctaacttta atagatgtcg acataaaatg
     6781 ggtcaatgat atctatctaa acaatcataa aattggagga atccttactg aagcaatgac
     6841 ctctgtagaa actggcttag tcacagatat cattattgga gtaggtatca atttcactat
     6901 taaagacttc cctcaggaat taaaagaaaa agctgccagc ttatttaaag ctacagctcc
     6961 tataacaagg aatgaattga tcatagaaat ctggcgtgct ttcttcgaaa caccagcaga
     7021 agagctatta tacctataca aaaaacagtc attcattcta ggaaaagaag tcactttcac
     7081 actagagcaa aaagactaca agggacttgc taaagacatc tcagaaaatg gaaaactttt
     7141 agttcaatgt gataacggaa aagaaatctg gctaaatagt ggcgaaattt ctctcaatag
     7201 ttggaagtaa aataacacaa ttataatata aacgatataa aaataacttc agattagtaa
     7261 ttcaattaag ttttacggat ctgaagtttt attggctcta aaaataaaaa agagagttac
     7321 agactctcat taaaacggag aataagggat tcgaaccctt gcgccagtta cccgacctaa
     7381 cgatttagca aaccgtcctc ttcagcctct tgagtaattc tccaattaat gggcacgagt
     7441 ggactcgaac caccgacctc acgcttatca ggcgtgcgct ctaaccaact gagctacgcg
     7501 cccaagttaa aaaacttggt aatttgaaca aagttcaaag cgggtgacga gaatcgaact
     7561 cgcgacaaca gcttggaagg ctgtagtttt accactaaac tacacccgca taaatactat
     7621 caataaaatg gcgcgagacg gaatcgaacc gccgacacat ggagcttcaa tccattgctc
     7681 taccaactga gctaccgagc cttattgcgg gagcaggatt tgaacctacg accttcgggt
     7741 tatgagcccg acgagctacc gag
//
LOCUS       DAVBDG010000077         7730 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBDG010000077 DAVBDG010000000
VERSION     DAVBDG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.078"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            447..632
                     /locus_tag="VQ526_001820"
                     /pseudo
     CDS             447..632
                     /locus_tag="VQ526_001820"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1244..1528
                     /locus_tag="VQ526_001821"
     CDS             1244..1528
                     /locus_tag="VQ526_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555512.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1521..1759
                     /locus_tag="VQ526_001822"
                     /pseudo
     CDS             1521..1759
                     /locus_tag="VQ526_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1855..2073
                     /locus_tag="VQ526_001823"
     CDS             1855..2073
                     /locus_tag="VQ526_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555513.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(2445..4769)
                     /gene="pflB"
                     /locus_tag="VQ526_001824"
     CDS             complement(2445..4769)
                     /gene="pflB"
                     /locus_tag="VQ526_001824"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2555514.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            5164..6225
                     /gene="dinB"
                     /locus_tag="VQ526_001825"
     CDS             5164..6225
                     /gene="dinB"
                     /locus_tag="VQ526_001825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2555515.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(6372..7217)
                     /locus_tag="VQ526_001826"
     CDS             complement(6372..7217)
                     /locus_tag="VQ526_001826"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2555516.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            7473..7730
                     /locus_tag="VQ526_001827"
                     /pseudo
     CDS             7473..7730
                     /locus_tag="VQ526_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2519 a         1462 c         1456 g         2293 t
ORIGIN      
        1 tgacgaagtc agtaacatct atacggtaag gcgacgctga cgtggtttga agagattttc
       61 gaaaagtatt agttaaatct tgtttaatat ctctacacca tactatttta catatattat
      121 ttagcgccac atagtactga actctctcca aaaacggtta tgcctctttg aatagggcgt
      181 tatcacaaga aaagcatctc cacgtttcaa cttcatatgg ctcaaaaaca atcaattgat
      241 gctaaaacct gtacctagat gtttcggttc ataaaaccat gaaactgtaa aagtggatga
      301 aattgatagc gatagtcaaa tcaagaggca tcataactct aaaaagtcac aatatataag
      361 ttcatcctcg gaaaaatatc attctaattg ttgaaatgga tatataaaca ggggtattaa
      421 cattaaatgc tcatgaaaca acgaatacag gtatcaaaac tatgacaaaa caagtcccta
      481 aatttactaa agacactgct caactttaca cctgtaaatg gttgttgtat aataaagtta
      541 caaagatgta cgaccacact gttgtaaatc atagtgttcg cgaatatatt actgatagca
      601 tttctacaaa tacaattgaa gaggctggat aggctttaaa cgaaatatgt cagtgttttg
      661 gcattcctag ccttaatatc atttaaagaa ttgtatagat aaaatttttt ccaatacaga
      721 cactcgtaac aactgcttca tttttctacc aacatattta ggaacaggat aagatacaag
      781 aggattaatc cataaatcag ttctatacca atctaagaca aataagctaa aaaaacgatt
      841 gataataagc aaatagattc caaattttct ctatctgctc attttaataa acaatactag
      901 tgtaactatc cttccagtca gaaacttgtc aaatcacacc gaaaactctt ctaaaattta
      961 tctcgttagg caatcaagca aaaactcgac gatagtacaa acattatcat acagaattga
     1021 ctgcctaaat tatatacttt agtaaggttt tcggataaga aaaaaggttc attttacatt
     1081 tctaaacatt cttttctaag atgaaaaaca gaatttttcg attgtgattt aaagcaacaa
     1141 gaagattttc agtatcatcc tatagatacg agctaattaa gaaaaactac atttttgaat
     1201 ataaactaca ataatataaa ctaaatttta taggaggaag acaatggatt ggtacgatta
     1261 tatgatacag gcatccaaac aatcacaatt caacgcaagc cattggtttc gctatttgcg
     1321 aaaagttatt tttgaagact attcttattt aacaaaccaa gatgtagaaa agttgctaga
     1381 ctccaaagaa ctaacccgtt ttcaaaaaat tagcttgaag tatgcctttc aagagcatac
     1441 tccaactcat aaatatgtga tttcattaaa taaacctgct aagttaacca atgttcaaaa
     1501 attgatggag aaatacaaac atggataaaa tgaaaccggt cttccaagcc ctaaataaga
     1561 aattaattca ggaaaatctg actttaacaa ttatggttat gtcttagaat atcatggttt
     1621 acgtgccaca caagatgttg atgcttttta tgaccaaaat cagaaaatca atgaaattat
     1681 tgctcgtgta ggaaaacagt ttaatcttaa catccacgaa gagttatggc tcaacaatca
     1741 tgttgcaaag caaatatgaa caaacaacct cccttaagtt tatgcgaatc cttgtactca
     1801 tttgagaatc tgaccgtttt agtagtccct attgagtatg tgttaggaat gaagatgatg
     1861 agtatcagag agcaggattt gcaagatatt ggtgcaatta ttaaatacaa aaactttcat
     1921 tcaccattcg atacctttaa atatttaaaa gacatgggat ttgatactat tgacctctct
     1981 gttctattag aaggttttag ttatgcttat ggtatggatt ggttagaaaa attttttaaa
     2041 gaaaatcaag ataaacttcg cgaattttat taacagtatc aaacaagatg attgtcaatt
     2101 agccagtcgt cttattttta tgatgaaatg aaatgaaata aaatcggaac aaatcactta
     2161 gagaattcca gtcaatttct agcactgttt gagccgtttc agcggactat agtaaaatga
     2221 aataagaata ggacaaatcg atcaggacag tcaaatcgat ttctaagaat gttttagaag
     2281 taaaggtgta ctattctagt ttcaatctat tatatttcag attcggtcca ctgttccttc
     2341 ccaaaaacat tgcatattgc gccgaaggaa ttgctcatac tatcccaagc acaaaaccct
     2401 cagtcgtttg accgagggtt cctttttatt attcaagaac ttgattagct caatgcatcc
     2461 aaggcgtcat ccattgaaag aacttcgtgg aagacacgtt gtgtcaattc agttttttgt
     2521 tctggagtga ggtatttagt gtttacacag tatccagaga tacgtacgat aacgtcttcg
     2581 cctgacatga ttttttcgta aacatcgttc aagtccataa cgttcaagtt aacgtgttgt
     2641 ccaccgtttt cgaagtaacc atcaaggatt gttaccaagt tatcaacttg ttcatcacga
     2701 gtcttaccaa gagcgcgagg tgatacttgt gtagtcagtg agataccgtc agctgcataa
     2761 ctaaagtcaa ggctagaaag tgagttcaag ttttgcaacc aaccaccttt agctttgtta
     2821 gatgggttag cacctggtga gaagaattca agtttagaca agttcacaga accatcttcg
     2881 ttgaggtata cacctttgtg aactggtgag ttaccagttt gtttagagta agcaacgtta
     2941 gatgtgattg tcaaaagtga tactgtagct tctgcgtctt tgtatagttt gtggctacgt
     3001 agacgagttg tgtaagcttc gatcaaccat tctgccaatt cgtttgaacg tgggtcatct
     3061 tcaccccagc gtgggtagtc accgattgtt tcgtaatcgt agatgtagcc atcttcgtca
     3121 cggattggtt taactgtagc gtatttgata gctgacaatg tatcaacagt gttagcaaat
     3181 ccacagatac cgaatcccat gttggcacgt tgtttagttg gcaagaaggc catttgaaca
     3241 gcttcgtagt tgtacctatc agtcatgtag tggatgatgt tcaaggcatc tacgtaagtg
     3301 tcagtcaacc agtcaagaga tttttcaaag ttcgctttaa ctgattcgaa ttcaagaact
     3361 tcgtcacgaa taggatcgat gtcaaatact ttgtagtctt tgtgaacatc gtcgtaacca
     3421 ccattcaaac cagtaagaag ggctttaaga acgtttacac gagcaccgaa gtactggatg
     3481 ttgtggcgtt gttcttcatt ttctggatca agtggagaca cacagcatga gatacagctc
     3541 atttcaccat atccgtcttt agccattgtt gttacacctt cgtattggat agaagagtgt
     3601 ttgtggctca tgtgcataca gtagcgacgg aagttgtatg gcaatttgtc agtccaaaga
     3661 actgtcaagt ttggttctgg tgagttaccg atgttgtcaa gagtgttcaa gaaacggtag
     3721 tccatcttag taacacggtg acgaccgtcg ttacccatac cagccataga agttgtgata
     3781 aaggttgggt cacctgagta caattggtca taagcttttg tacgagcaaa tttaactgta
     3841 cgaagtttca taacgaaatc atcaacgaat tcttggattt ctgattcagt aaatgtacca
     3901 cgagcaaggt cacgttctgc aaagatgtcc aatacgattg gtacacgacc tagagatgta
     3961 gcagcaccgt taatcacacg gcagacagcc atgaaagcaa tgttaaccca ttggattgct
     4021 tctttcacgt tcatcgctgg tttgcgaaca tcaaccccgt aaaggtcacc caggcgaaca
     4081 acttgttgca atgcttggta ttgaaggttt acttcttcac gaagacggat tgtttcttca
     4141 tcgatttctt tgattgcatt ccagtcgttt actttttctt gcatcaagta gtctgcaccg
     4201 taaagagcaa gacgtgcgta aacaccgatg atacgtccgc gtgagtatgc atctggaaga
     4261 ccagttacag tgtgtgcatg acgagcgcga cgaatatttg aagtgtaggc acggaaaata
     4321 ccgtcgttaa ctgttgttac atatttagtg aagatttcgt gaacagctgg gtctggttcg
     4381 tatccatttt cttttaaagt agtttcagcc atacggatac caccttttgg catgaagttc
     4441 aatttgaaga gttcatcatt ttggatacca aagataactt cattttcttt gtcgataaat
     4501 ccagcaggga tatcagcgat agatgttgga cgagtgtcca ttgggaaacg agtttcttcg
     4561 tagtgtgctt tagtttcttc tacaattttc ttgatgtgaa gtgaacgctc tgttggtcct
     4621 gcaaggaagc tttcgtctcc atcataaggt gtgtagttag cttgtacaaa gcgtgataca
     4681 cttgcttttt ctttccaatc tacgcctttg aagccttccc aagctttgtc aaaaatatct
     4741 tgtgcttcaa caaccgtctt aacaaccata ttaatgtcct cttttttcta gtaacagtca
     4801 tctgttacat tcataagaca agtataccat aaagtaatcg atttcaacaa gtgcaaaatc
     4861 cctattttta cactttcttt tctaaaacag tctatatttt atttcaaact gtattatatt
     4921 tttgaaaaaa ataaagtcct tttttctttt tttcagaaaa aagggtataa taaaagaaaa
     4981 taagcagtaa cactcaatgg aaatcgaaaa gcaaactagg aagctagccg caggctgctc
     5041 aaagcactgc tttgaggttg taaatagaac tgacgaagtc agctcaaaat actgttttga
     5101 ggttgcagat ggaagctgac gtggtttgaa gagattttcg aagagtataa aaaggtgcta
     5161 ggcatgttga tttttccttt gttaaatgat ttgtcaagaa aaatcatcca tattgacatg
     5221 gatgcctttt ttgctgcggt ggaaatcaag gataatccta aactcagagg aaaacctgtc
     5281 attattggaa gcgaccctcg gcaaacaggt ggacggggag tcgtttctac ctgtagctat
     5341 gaggctcgag cttttggtgt ccattctgcc atgagttcca aggaagctta tgaacgttgt
     5401 ccccaggccg tctttatctc aggaaattat gagaaataca aagctgtggg actccagatt
     5461 cgagctattt ttaagcgcta tacagatttg attgaaccca tgagcattga cgaagcctat
     5521 ttggatgtga cagaaaataa actcggtatc aagtcagcgg tcaaaattgc tcgcctcatt
     5581 caaaaagata tctggcaaga actccatcta actgcttctg ccggtatttc ctacaacaag
     5641 ttcttagcta aaatggcgag tgattatcaa aaacctcatg gtttgacagt gattctacct
     5701 gaacaggctg agaattttct caaacaaatg gatatttcca aatttcatgg agtaggaaaa
     5761 aagacagtag aacgtcttca tcaaatgggc gtttttactg gtgctgattt acttgaagtt
     5821 cctgaggtga ccctaataga ccgttttggt agactaggct atgatctgta tcgaaaggct
     5881 cgtggcattc acaactctcc agtcaaatcc aatcgcatcc gtaaatcaat cggcaaggag
     5941 aaaacctacg ggaagattct ccgtgctgag gaagatatca aaaaagagct gactcttcta
     6001 tcagaaaaag tcgctctcaa tctacatcaa caagaaaaag ctggaaaaat tgtcattttg
     6061 aaaatccgct acgaggactt ttcaactctt accaaacgaa aaagtcttgc tcaaaaaaca
     6121 caagatgcta gtcagataag ccaaatagcc ctgcaactct atgaagaatt aagcgagaaa
     6181 gaaagaggtg tccgcctatt ggggattacc ctgactggat tttaaagctc aatgaaaatc
     6241 aaagagcaaa ctaggaagct agccgcaggc tgctcaaaac actgttttga ggttgcagat
     6301 aaagctgacg cggtttgaag agattttcta agagtataaa accttgaaga gttgcccctt
     6361 caaggttttt cttatacaaa taaacggaca aaactgtaaa gtagcaaaac actaccaagc
     6421 acgatacggt atttaccaaa aagggtgaag tcgtgttttt tcacatagct ggtcaagaag
     6481 cgaatagcca ccatgctgac cgcaaaagct actcccatcg cgaccaagag caaaaacaat
     6541 tgcccaaagc tcaagagttc tccggctttc acaaatttga aaatctttaa ggcactagct
     6601 ccaaacataa cgggaatccc aagatagaag gtaaattctg tcacaactga acgactggtt
     6661 ccatttaaca aaccaccgac aatcgttgca cctgaacggc tagtccctgg taaaagagca
     6721 agaacttgga agagtccgat atagaaagcg gtcgtataag gaagcttgtc caactctgtt
     6781 acacttggct cgatagcacg cgctttattg cgcttttcca aatagatgaa ggcaaccccg
     6841 tagataatca acatgagagc aactgaaacc atgttatgga agtgggtatc aaaccaatca
     6901 tcaaatttaa agacaccaag taaaggcaaa gtggcaatca agaccttcaa ccatagtctc
     6961 caagtcttac gaacttcctg tttgtcctta gtcggtttaa aaggattgag cttgttaaaa
     7021 taaatcacca taactgctaa aatagcacca agctgaatca cgacattaaa catggacata
     7081 aaggcttcat tttgattttg gtattggata aactcctctg ctaaaatcaa gtgacctgta
     7141 ctggaaatcg gcaaccattc cgtaattcct tcaacaatcc cgaagaagat agattttaaa
     7201 atttcaataa gatacatata tttctcctta tattatattt aacgcagtta gctactgcca
     7261 aagcccaagt ggtatacttg gaataagcca ctgtggatta gttcatttcc tccaatcgct
     7321 tgacgatttc cgaaagggtc taacaacagc tttttgtttc aattggtaat gagtttgata
     7381 acgcatagac gttttatctc gtctaaggta acaaggcaaa aagttcgtgg ataaccaagt
     7441 agacttgaaa tactatatct ggaggtactc ccatgcttta tgtgggcatt gatgtcgcta
     7501 aaaataaaca cgatgttaca gccttgaatg ttcgaggaaa actgttctta aaccactcac
     7561 tttttcaaat aataaagctg gttttgaact cttagatctg tcttttcgac agctcaacca
     7621 agactgtctc atcgctctta aacttctttc tgaccccaat cgtgaacaat ttcaacacga
     7681 taatcggcaa gtagaactaa aaatactggc tagacatatt catcgtctca
//
LOCUS       DAVBDG010000078         7339 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBDG010000078 DAVBDG010000000
VERSION     DAVBDG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.079"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..116
                     /locus_tag="VQ526_001828"
     CDS             <1..116
                     /locus_tag="VQ526_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2555517.1"
                     /translation="TPEQAKQILEYGVRGIVVGGAITRPKEITERFVASLK"
     gene            135..1652
                     /locus_tag="VQ526_001829"
     CDS             135..1652
                     /locus_tag="VQ526_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2555518.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            1777..3105
                     /locus_tag="VQ526_001830"
     CDS             1777..3105
                     /locus_tag="VQ526_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2555519.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            3358..4224
                     /locus_tag="VQ526_001831"
     CDS             3358..4224
                     /locus_tag="VQ526_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2555520.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            4240..5079
                     /locus_tag="VQ526_001832"
     CDS             4240..5079
                     /locus_tag="VQ526_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2555521.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            5351..5803
                     /locus_tag="VQ526_001833"
     CDS             5351..5803
                     /locus_tag="VQ526_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2555522.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            5900..6244
                     /locus_tag="VQ526_001834"
     CDS             5900..6244
                     /locus_tag="VQ526_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555523.1"
                     /translation="MVSSKHAARASFLDGQGITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            6528..7172
                     /locus_tag="VQ526_001835"
     CDS             6528..7172
                     /locus_tag="VQ526_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2555524.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
BASE COUNT         2252 a         1301 c         1492 g         2294 t
ORIGIN      
        1 atacaccaga acaagccaaa caaatccttg aatatggagt gcgaggcatc gttgttggtg
       61 gcgccattac tagaccaaaa gagattacag aacgcttcgt tgctagtctt aaataagatg
      121 tgagggggag ttttatgttt aaagttttac aaaaagttgg aaaagctttt atgttaccta
      181 tagctatact tcctgcagca ggtctacttt tggggattgg tggtgcactt tcaaacccaa
      241 ccacgatagc aacttatcca atactagaca atagtatttt tcaatcaata ttccaagtaa
      301 tgagctctgc aggagaggtt gtattcagta atttgtcact acttctctgt gtgggattat
      361 gtattggctt agcgaaacga gataaaggaa ccgctgcgtt agcaggagta actggttact
      421 tagttatgac tgcaacgatc aaagctttgg taaaactttt tatggcagaa ggatctgcaa
      481 ttgatactgg agttattgga gcattagttg tcggaatagt tgccgtatat ttgcacaacc
      541 gatataacaa tattcaatta ccttccgctt taggattctt tggaggttca cgcttcgttc
      601 ctattgttac atcgttctct tctatcttga ttggctttgt cttctttgtt atttggccac
      661 ctttccaaca acttcttgtt tctacaggtg gatatatttc tcaggcgggt ccaattggaa
      721 cttttctata tggattttta atgagacttt ctggagcagt aggcttacat catataattt
      781 accctatgtt ttggtatact gaacttggtg gtgttgagac tgttgcagga caaacagtgg
      841 ttggagctca aaaaatattt tttgctcaat tagccgatcc ggcccattct ggattattta
      901 cagaaggaac aaggtttttt gcaggtcgtt tctcaacaat gatgttcggt ttaccggctg
      961 cctgtttagc gatgtaccat agtgttccta aaaatcgtcg taaaaaatac gcgggtttgt
     1021 tttttggagt tgctttaaca tcttttatta ccggtattac agaaccaatt gaatttatgt
     1081 ttctattcgt cagtccggtt ctatatgttg ttcacgcatt ccttgatggt gttagcttct
     1141 ttattgcaga cgtcttaaat atttcaatag gaaacacatt ttcaggaggt gtaatcgatt
     1201 tcactttatt tggaattttg caggggaacg ctaagacgaa ttgggttctt cagattccaa
     1261 ttggacttat ttggagtgtt ttgtattata ttatttttag atggttcatt actcaattca
     1321 acgttctaac gccaggacga ggagaagaag tagattctaa agaaatttct gaatccgcag
     1381 attcaacttc aaatactgca gattatttaa aacaggatag cctacaaatt atcagagcct
     1441 tgggtggatc aaataatata gaagatgtag atgcttgtgt gacacgttta cgtgtagctg
     1501 taaaagaagt taatcaagtt gataaagcac ttttaaaaca aattggtgca gttgatgtct
     1561 tagaagtgaa gggtggcatt caagcaatct atggagcaaa agcaatctta tataaaaata
     1621 gtattaatga aattttaggt gtagatgatt aagtacttac tgacttaata aaaaacagag
     1681 gagagtgatg gatgagtagg atgaaatgaa atcgcataca agaaataaag aactcattat
     1741 ccaagttgga tacgcttatt acataggaga atacaaatga aatttagaaa attagcttgt
     1801 acagtacttg cgggtgctgc ggttcttggt cttgctgctt gtggcaattc tggcggaagt
     1861 aaagatgctg ccaaatcagg tggtgacggt gccaaaacag aaatcacttg gtgggcattc
     1921 ccagtattta cccaagaaaa aactggtgac ggtgttggaa cttatgaaaa atcaatcatc
     1981 gaagcgtttg aaaaagcaaa cccagatata aaagtgaaat tggaaaccat cgacttcaag
     2041 tcaggtcctg aaaaaatcac aacagccatc gaagcaggaa cagctccaga cgtactcttt
     2101 gatgcaccag gacgtatcat ccaatacggt aaaaacggta aattggctga gttgaacgac
     2161 ctcttcacag atgaatttgt taaagatgtc aacaatgaaa acatcgtaca agcaagtaaa
     2221 gctggagaca aggcttatat gtatccgatt agttctgccc cattctacat ggcaatgaac
     2281 aagaaaatgt tagaagatgc tggagtagca aaccttgtaa aagaaggttg gacaactgat
     2341 gattttgaaa aagtattgaa agcacttaaa gacaagggtt acacaccagg ttcattgttc
     2401 agttctggtc aagggggaga ccaaggaaca cgtgccttta tctctaacct ttatagcggt
     2461 tctgtaacag atgaaaaagt tagcaaatat acaactgatg atcctaaatt cgtcaaaggt
     2521 cttgaaaaag caactagctg gattaaagac aatttgatca ataatggttc acaatttgac
     2581 ggtggggcag atatccaaaa ctttgccaac ggtcaaacat cttacacaat cctttgggca
     2641 ccagctcaaa atggtatcca agctaaactt ttagaagcaa gtaaggtaga agtggtagaa
     2701 gtaccattcc catcagacga aggtaagcca gctcttgaat accttgtaaa cggatttgca
     2761 gtattcaaca ataaagacga caagaaagtc gctgcatcta agaaattcat ccagtttatc
     2821 gcagatgaca aagagtgggg acctaaagac gtagttcgta caggtgcttt cccagtccgt
     2881 acttcatttg gaaaacttta tgaagacaaa cgcatggaaa caatcagcgg ctggactcaa
     2941 tactactcac catactacaa cactattgat ggatttgctg aaatgagaac gctttggttc
     3001 ccaatgttgc aatctgtatc aaatggtgac gaaaaaccag cggatgcttt gaaagccttc
     3061 actgaaaaag caaacgaaac aatcaaaaaa gctatgaaac aatagtcctt agttattcta
     3121 tgaaaaagta attttttaaa gaacctaaga gtgtataccc ccttttccct ctacacagat
     3181 agtgtaagaa aagggggctt ttgtttaaaa tgtaagaaac tgtcacgaaa ttaaaatgaa
     3241 gttcttacat aagcgaatca taaaaaattt cattttgatt ttaaaacagt tcaagaaagt
     3301 caaaaaatta ttctatttga aagagaggtg ccgactgtga aagtcaataa aatccgtatg
     3361 cgggaaacag tgatttccta cgctttccta gcaccagtat tattcttctt tgtcatcttt
     3421 gtgttggctc cgatggtgat gggcttcatt acaagtttct ttaactactc aatgactaaa
     3481 tttgagtttg taggcttgga taactatatc cgtatgttta aagatcctgt ctttacaaaa
     3541 tctctgatta acacagttat tttggttatt ggatctgtac cagttgttgt tctattctca
     3601 ctctttgtag catctcagac ctatcatcaa aatgtcattg ccagatcctt ctaccgtttc
     3661 gtcttcttcc ttcctgttgt aacgggtagt gttgccgtga cagttgtttg gaaatggatt
     3721 tatgacccac tatcagggat tctaaacttt gtccttaagt caagccacat catcagccaa
     3781 aacatttctt ggttgggaga taaaaactgg gcattgatgg cgattatgat tattctcttg
     3841 accacttcag ttggtcagcc catcatcctt tatatcgctg ccatggggaa tattgacaat
     3901 tcactggttg aagcggcgcg tgttgatggt gcaactgagt ttcaagtttt ctggaagatt
     3961 aaatggccaa gccttcttcc aacaactctt tatattgcaa tcatcacaac aattaactca
     4021 ttccagtgtt tcgccttgat tcagcttttg acatctggtg gtccaaacta ctcaacaagt
     4081 accttgatgt actaccttta cgaaaaagcc ttccaattga cagaatacgg ctatgccaac
     4141 acaattggtg tcttcttggc agtcatgatt gctatcgtaa gctttgttca atttaaagta
     4201 cttggaaacg acgtagaata ctaaagaaag gagacagcta tgcaatctac agaaaaaaaa
     4261 ccattaacag cctttactgt tatttcaaca atcattttgc tcttgttgac tgtgctgttc
     4321 atctttccat tctactggat tttgacaggg gcattcaaat cacaacctga tacaattgtt
     4381 attcctcctc agtggttccc taaaatgcca accatggaaa acttccaaca actcatggtg
     4441 cagaaccctg ccttgcaatg gatgtggaac tcagtattta tctcattggt aaccatgttc
     4501 ttagtttgtg caacctcatc tctagcaggt tatgtattgg ctaaaaaacg tttctatggt
     4561 caacgcattc tatttgctat ctttatcgct gctatggcgc ttccaaaaca agttgtcctt
     4621 gtaccattgg tacgtatcgt caacttcatg ggaatccacg atactctctg ggcagttatc
     4681 ttgcctttga ttggatggcc attcggtgtc ttcctcatga aacaatttag tgaaaatatt
     4741 cctacagagt tgcttgaatc agctaaaatc gacggttgtg gtgagattcg taccttctgg
     4801 agtgtagcct tcccgattgt gaaaccaggg tttgcagccc ttgcaatctt taccttcatc
     4861 aatacttgga atgactactt catgcaattg gtaatgttga cttcacgtaa caatttgacc
     4921 atctcacttg gggttgcgac catgcaggct gaaatggcaa ccaactatgg tttgattatg
     4981 gcaggagctg cccttgctgc tgttccaatc gtcacagtct tcctagtctt ccaaaaatcc
     5041 ttcacacagg gtattactat gggagcggtc aaaggataat actctgagaa aatctcttca
     5101 aactacgtca gcgtcacctt gccatactta agtattgcct gcggttagct tcctagtttg
     5161 ttcttcaatt ttcattgagt ataggaaaat caatctatca agatacagaa gtatatttta
     5221 tagatttaga gaatatagag gttataagtg tctacaaaat ggagggtatg cagttacttt
     5281 atgaagtttt gtcagacact tataaactta agaatggttt tagttaacta tcagaaacga
     5341 aggaaagagt atgatttttg acgatttgaa aaacatcacc ttttacaaag ggattcatcc
     5401 caatttagac aaggctatcg actatctcta ccaacatcgt aaagattcat tcgaattagg
     5461 aaagtatgag attgatggag ataaagtctt tctagttgtt caggaaaatg tcctcaatca
     5521 agttgagaat aatcaatttg aacaccataa gaactatgca gatttgcatt tgctggtaga
     5581 aggacatgaa tattcgagct acggttcacg tatcaaagac gaggcagtag cattcgacga
     5641 agcgactgac attggctttg ttcattgtca tgaacactac ccactcttgt tgggttatca
     5701 taattttgcg attttcttcc caggtgagcc acatcagcca actggctatg caggtatgga
     5761 agaaaaggtt cgaaaatatc tctttaaaat tttgattgat taaaaatagg atgaattgtt
     5821 tttttgtaaa gctttgacaa tactctacca tgaaattgat ctttgtgagg tagagaaatg
     5881 agaataaaat atttaaaaat tggtatcttc taagcatgct gcaagagcta gtttcttaga
     5941 tggacagggg attacagttg atgagatgtc ctggataatt aatggcattg tgaatgcatt
     6001 gattggtaga tacataaaat taggtactta tgcagctaag tatggtatta gtatagcacg
     6061 ctcgatctta agtagggcag ctgcaactgc agcagcaaga gtaggattac tgaccaagat
     6121 ttctggatgg attttacgag tagctgtgaa tgtagctgat gtatatggta attttgccaa
     6181 caatattgct gcagcttggg atgcatatga taaaattcct aacaatggtc gtataaactt
     6241 ttaaaatgcg agaatgaaag cactttgtat ttttttattg aatatgttag cttggacagt
     6301 gcttgcaatg ataattcgtg gagggctaga tggatttgat aggcatactt ggagtactat
     6361 tttaattgct tcgctgttcg gggtatatga ttataagccc atagataaaa atagaaaaaa
     6421 gtccaaaaga aaaaagaaaa aatagatttg ttcatggtag ggacttatga aagctttact
     6481 gacaaaaaag aaaacagttt acaaagaaaa atgatggagg agcaaacatg gcacaaaaag
     6541 gagtaagcct tatcaaggca gcatttgata cagataactt tctcatgcgt tttagtgaga
     6601 aggtcttgga catcgtgaca gccaatcttc tttttgtcgt ctcttgtttg cccatcgtga
     6661 cgattggagt ggctaaaatc agcctctacg agaccatgtt cgaagttaag aagagcagac
     6721 gggtgcctgt ttttaaaatc tatctaagat ctttcaagca aaatctgaaa ctaggtcttc
     6781 agttaggtct gcttgagttg ggcattgttt cattaagcct cctagatctt tatctctttt
     6841 ggggtcaaac agctctgccc tttcaattgc tgaaagctat ttgtctaggc attctgattt
     6901 tccttacgat tgtgatgttg gctagctatc caatcgcggc acgttatgat ttaacttgga
     6961 aagaaattct tcaaaaagga ttgatgttgg ctagttttaa ctttccttgg ttcttcctca
     7021 tgttagccat tcttgtcctc attgtgatgg ttctttatct gtctgccttc agtctactct
     7081 taggtggctc agtcttcctg ctttttgggt ttggactatt ggtctttatc cagactggat
     7141 tgatggagaa aattttcgca aaataccaat aggagcctta tttctgaaac tactttcaaa
     7201 ggctccaaac gctattctat aagcgagaaa ctaaaatcta agtgtctata agatattgaa
     7261 agccttttcg gtaaagtgta tactaaagaa gtaaagaaaa atctgcttta gcagaaaata
     7321 aaaataatag gagaaaatc
//
LOCUS       DAVBDG010000079         7074 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBDG010000079 DAVBDG010000000
VERSION     DAVBDG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.080"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(295..1986)
                     /locus_tag="VQ526_001836"
     CDS             complement(295..1986)
                     /locus_tag="VQ526_001836"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2555525.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2122..2568
                     /gene="argR"
                     /locus_tag="VQ526_001837"
     CDS             2122..2568
                     /gene="argR"
                     /locus_tag="VQ526_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2555526.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDKWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2619..5153
                     /gene="mutS"
                     /locus_tag="VQ526_001838"
     CDS             2619..5153
                     /gene="mutS"
                     /locus_tag="VQ526_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2555527.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5300..6994
                     /gene="patA"
                     /locus_tag="VQ526_001839"
     CDS             5300..6994
                     /gene="patA"
                     /locus_tag="VQ526_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HET2555528.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDNEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1925 a         1381 c         1667 g         2101 t
ORIGIN      
        1 ttggttaggt gaattagtga agcgtttagg caagtgtctc tggttacgac gtcatggcct
       61 ctaaatcgat tatatttagg ggtcatgact agtgaagcag ttagctagtt cgcatataag
      121 cggctagcgt ctaacaatta ggaactttag ttccaataac tttaagatta cgacgtttta
      181 ggacataaat cgatcatatt tatgtcctaa aactagtgaa gcgcctagcc aaagtccgaa
      241 taggatttgg cgttagttac ttagattgct ttgcaatcaa gtaactttgg cgatttacat
      301 cttctctggc gcttctactc caagcaagcg aagggcttct tttagaacga ctgcggttgc
      361 gtagctgagg gctagacggc tgtcgcgttc tgggctttca tccaagatac gtgtatgtgc
      421 atagtatttg ttaaaggatt gagctaggct aattgcaaat ttagcaatga tagaaggttc
      481 aaagttatct gccgcacggt tgataatacg tgggaagtct tgaatgagtt taatgatttc
      541 ccagctttca gtatcattca agctatagtt gccagctgtt tctggtttga aatcggcttt
      601 gcgtaagata gattggatac gagcgtaggc atattgaacg taaggtccag tttcaccctc
      661 gaaggatacc atagcttcta ggtcgaagtc gtatccattt gtacggtcgg ttttgaggtc
      721 atagaattta atggctccaa ccccaacagc atgtgctact tggtctttgt tttctagttc
      781 aggattttta gcctcgattt ggaccttggc acggctaaca gcctctgcaa cagtaggctc
      841 tagcaagatg acattccctt tacgagtaga gagcttcttc ccttcctttg taaccaaacc
      901 aaaaggaacg tgagtaatgt cgtcactcca gtcgtagccc atctcttgca agacagcttt
      961 gagctgttta aagtgggcag attgttcttg accaacgaca tagatagatt tagcaaattg
     1021 gtattcgttt ttacggtaaa gggctgcagc caagtcacgt gtgatataga gagttgcacc
     1081 atcagacttc ttgatgaggg ctggatgttc aattccatat ttctcaagat tcacaacttg
     1141 ggcaccttct gattcaagaa gtagtccttt ttcagaaaga atgtctacaa ctgcatccat
     1201 cttatcattg tagaaggctt ctccgttata gctgtcaaat tcaaccttca attcattgta
     1261 aaggcggtta aattccacta aactttcatc gcggaaccat tgccaaagag cgagagcttc
     1321 ctcatctcca ttttcaagtt tacggaacca ttcgcgcgct tcttcatcca agctagggtc
     1381 attttcagct tcagcgttga tgcggacata gagtttaagg agttcatcga ttggatgagc
     1441 ttttacagct tcttcgtcgc cccatttttt gtaggcaaca atcaacatcc caaattgttt
     1501 accccagtct cccaaatggt tgaccttgac cgtttgataa ccgatttttt ggaaaatatg
     1561 tgacaagcta tctccgataa cagttgaacg caggtggcca atagaaaatg gtttagcgat
     1621 attcggacta gacatgtcga taacaacatt ttcttgtttg ccaatatttt ggtcagcata
     1681 gtgttctttt tcagttgtaa cagcttgcaa tacttgagca gaaatggcag atttatcaag
     1741 gaaaaagtta acgtaaggtc ctgttgcgac aactttttca aaggcttggc tgttcatttt
     1801 ttcagccagt tcagccgcaa tcatttgtgg tgctttacgt tcgactttgg caagagaaaa
     1861 agcagggaaa gcgatgtcac ccatttctga gtttttaggg gtttccagta actttaaaat
     1921 agcctcttgg tccaggctat caatgatgct agccaattcg ctagcaatca attcttttgt
     1981 attcattaag agctcctttt tggacttttc tactatttta acacaatttt aaagaaagaa
     2041 gaaaaaattt ttgaaatctc ttgttttttt ggtataatat ggttataaat atagttataa
     2101 atatgcacgc aagaggattt tatgagaaaa agagatcgtc atcagttaat aaaaaaaatg
     2161 attactgagg agaaattaag tacacaaaaa gaaattcaag atcggttgga ggcgcacaat
     2221 gtttgtgtga cgcagacaac cttgtctcgt gatttgcgcg aaatcggctt gaccaaggtc
     2281 aagaaaaatg atatggtgta ttatgtacta gtaaatgaga cagaaaagat tgatttggtg
     2341 gaatttttgt ctcatcattt agaaggtgtt gcaagagcag agtttacctt ggtgcttcat
     2401 accaaattgg gagaagcctc tgttttggca aatattgtag atgtaaacaa ggataaatgg
     2461 attttaggaa cagttgctgg tgccaatacc ttattggtta tttgtcgaga tcagcacgtt
     2521 gccaaactca tggaagatcg tttgctagat ttgatgaaag ataagtaagg tcttgggagt
     2581 tgctctcaag acttattttt gaaaaggaga gacagaaaat ggcgatagaa aagctatcac
     2641 ccggcatgca acagtatgtg gatattaaaa agcaatatcc agatgctttt ttgctctttc
     2701 ggatgggtga tttttatgaa ttattttatg aggatgcggt caatgctgcg cagattctgg
     2761 aaatttcctt aacgagtcgc aacaagaatg ccgacaatcc gatccctatg gcgggtgttc
     2821 cctatcattc tgcccaacag tatatcgatg tcttgattga gcagggttat aaggtggcta
     2881 tcgcagagca gatggaagat cctaaacaag cagttggggt tgttaaacga gaggttgttc
     2941 aggtcattac gccagggaca gtggtcgata gcagtaagcc ggacagtcag aataattttt
     3001 tggtttccat agaccgcgaa ggcaatcaat ttggcctagc ttatatggat ttggtgacgg
     3061 gtgactttta tgtgacaggt cttttggatt tcacgctggt ttgtggggaa atccgtaacc
     3121 tcaaggctcg agaagtggtg ttgggttatg acttgtctga ggaagaagaa caaatcctca
     3181 gccgccagat gaatctggta ctctcttatg aaaaagaaag ctttgaagac cttcatttat
     3241 tggatttgcg attggcaacg gtggagcaaa cggcatctag taagctgctc cagtatgttc
     3301 atcggactca gatgagggaa ttgaaccacc tcaaacctgt tatccgctac gaaattaagg
     3361 atttcttgca gatggattat gcgaccaagg ctagtctgga tttggttgag aatgctcgct
     3421 caggtaagaa acaaggcagt cttttctggc ttttggatga aaccaaaacg gctatgggga
     3481 tgcgtctctt gcgttcttgg attcatcgcc ccttgattga taaggaacga atcgtccaac
     3541 gtcaagaagt agtgcaggtc tttctcgacc atttctttga gcgtagtgac ttgacagaca
     3601 gtctcaaggg tgtttatgac attgagcgct tggctagtcg tgtttctttt ggcaaaacca
     3661 atccaaagga tctcttgcag ttggcgacta ccttgtctag tgtgccacgg attcgtgcga
     3721 ttttagaagg gatggagcaa cctactctag cctatctcat cgcacaactg gatgcaatcc
     3781 ctgagttgga gagtttgatt agcgcagcga ttgctcctga agctcctcat gtgattacag
     3841 atgggggaat tatccggact ggatttgatg agactttaga caagtatcgt tgcgttctca
     3901 gagaagggac tagctggatt gctgagattg aggctaagga gcgagaaaac tctggtatca
     3961 gcacgctcaa gattgactac aataaaaagg atggctacta ttttcatgtg accaattcgc
     4021 aactgggaaa tgtgccagcc cactttttcc gcaaggcgac gctgaaaaac tcagaacgct
     4081 ttggaaccga agaattagcc cgtatcgagg gagatatgct tgaggcgcgt gagaagtcag
     4141 ccaacctcga atacgaaata tttatgcgca ttcgtgaaga ggtcggcaag tacatccagc
     4201 gtttacaagc tctagcccaa ggaattgcga cggttgatgt cttacagagt ctggcggttg
     4261 tggctgaaac ccagcatttg attcgacctg agtttggtga cgattcacaa attgatatcc
     4321 ggaaagggcg ccatgctgtc gttgaaaagg ttatgggggc tcagacctat attccaaata
     4381 cgattcagat ggcagaagat accagtattc aactggttac agggccaaac atgagtggga
     4441 agtctaccta tatgcgtcag ttagccatga cggcggttat ggcccagctg ggttcctatg
     4501 ttcctgctga aagcgcccat ttaccgattt ttgatgcgat ttttacccgt atcggagcag
     4561 cagatgactt ggtttcgggt cagtcaacct tcatggtgga gatgatggag gccaataatg
     4621 ccatttcgca tgcgaccaag aactctctca ttctctttga tgaattgggg cgtggaactg
     4681 caacttatga cgggatggct cttgctcagt ccatcatcga atatatccat gagcacatcg
     4741 gagctaagac cctctttgcg acccactacc atgagttgac tagtctggag tctagtttac
     4801 aacacttggt caatgtccac gtggcaactt tggagcagga tgggcaggtc accttccttc
     4861 acaagattga accgggacca gctgataaat cctacggtat ccatgttgcc aagattgctg
     4921 gcttgccagc agacctttta gcaagggcgg ataagatttt gactcagcta gagaatcaag
     4981 gaacagagag tcctcctccc atgagacaaa ctagtgctgt cactgaacag atttcactct
     5041 ttgatagggc agaagagcat cctatcctag cagaattagc taaactggat gtgtataata
     5101 tgacacctat gcaggttatg aatgtcttag tagagttaaa acagaaacta taaaaccaag
     5161 actcactagt taatctagct gtatcaagga gacttctttg acaattctcc acttttttgc
     5221 tagaataaca tcacacaaac agaatgaaaa ggagctgacg cattgtcgct cccttttgtc
     5281 tattttttaa ggagaaagta tgctgattca gaaaataaaa acctacaagt ggcaggccct
     5341 ggcttcgctc ctgatgacag gtttgatggt tgctagttca cttctgcaac cgcgttatct
     5401 gcaggaagtc ttaggcgccc tccttactgg gaaatatgaa gctatttata gtatcggggc
     5461 ttggttgatt ggtgtggccg tagtcggtct agttgctggt ggactcaatg ttgtcctcgc
     5521 agcctatatt gcccaaggag tttcatccga ccttcgggag gatgccttcc gtaaaattca
     5581 aaccttttct tatgctgata ttgaacaatt taatgcggga aatctagtcg ttcgaatgac
     5641 aaatgatatc aaccagattc agaacgttgt catgatgacc ttccagattc ttttcagact
     5701 tcccctcttg ttcatcggtt cgtttatcct agcggttcaa accttacctt ctctgtggtg
     5761 ggtgattgtt ctcatggtag tcttgatttt tggtttgact gctgtcatga tgggaatgat
     5821 ggggcctcgt tttgccaagt ttcaaaccct tcttgagcgc atcaatgcca ttgccaagga
     5881 aaatttacgt ggcgttcgtg tggtcaagtc ctttgtccaa gaaaaagagc aatttgctaa
     5941 gtttacagag gtctcagacg agcttcttgg tcaaaacctt tacattggtt atgccttttc
     6001 agtagtggaa ccctttatga tgttggttgg ttacggggcg gtcttcctct ctatttggct
     6061 ggtcgcggga atggttcagt cggatccgtc tgttgttggt tccatcgctt cttttgttaa
     6121 ttacctaagc cagattatct ttaccattgt tatggttgga tttttgggaa attctgtcag
     6181 ccgtgccatg atttccatgc gtcgtattcg agaaattctt gacgcagagc cagctatgac
     6241 cttcaaggat atcccagatg aagagttggt tggaagcctt agctttgaaa atgtgacctt
     6301 tacctatcca atggacaatg aaccgatgct gaaagatgtg agctttacta ttgaacctgg
     6361 tcaaatggtt ggtgtagttg gagcgactgg tgcaggaaag tcaaccttgg ctcaattgat
     6421 tccacgtctc tttgatccac agaacggggc cattaaaatc ggtggcaagg atattcgaga
     6481 agtgagtgaa ggaaccctgc gtaaaacagt ttccatcgtt ctccaacgtg ccattctttt
     6541 tagtggaacg attgcagata acttgagaca ggggaaggga aatgctactc tatttgaaat
     6601 ggagcgcgca gccaatattg cccaggctag tgaattcatt catcgtatgg agaaaacctt
     6661 tgaaagtcca gttgaagaac ggggaaccaa tttctctggt ggacaaaaac aaaggatgtc
     6721 gattgcgcgt gggattgtca gcaatccacg tattctgatt tttgacgatt cgacctcagc
     6781 cttggatgcc aaatcagagc gcttggtgca agaagctttg aataaggact tgaaggggac
     6841 gacaaccatt attattgctc aaaaaattag ctcggttgtc catgcagaca agatcttggt
     6901 tctaaatcaa ggacgattga ttggtcaagg cacgcatgca gacttggttg ccaacaatgc
     6961 cgtttaccgt gaaatctatg aaacacagaa atgaaagaca aactataaga aaagtcaata
     7021 gttttatcta aattatttct tatttcaatt tgatgatttg gcgatgattt taga
//
LOCUS       DAVBDG010000080         6991 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBDG010000080 DAVBDG010000000
VERSION     DAVBDG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.081"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            226..6654
                     /locus_tag="VQ526_001840"
     CDS             226..6654
                     /locus_tag="VQ526_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2555529.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDNNTSNEEARIKE
                     DSNQSQGDNAHSSANKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSSLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANHADEKG
                     MISYYLVDPDKDASKYQKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEESSVEGE
                     ASLEINKTISTIREFENKDLKKLIKKKYKEEDDFVTGGKRTVELDYKYDDKGNITAYE
                     DESALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEIK
                     ATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRFFVKDDDRIKAETKIRMPEKNKETK
                     AEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYA
                     LKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQA
                     VLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSN
                     VEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWEL
                     RVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGN
                     VYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNN
                     GFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGE
                     VSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDA
                     GYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQR
                     EDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGI
                     FLGLKKKNQD"
BASE COUNT         2764 a          945 c         1346 g         1936 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg caggttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttatataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaagcatct ctgataaaga aaaagtagta
      361 gtagataagg aaacagaaaa taaagagaaa cataaagata ttcataatgc tatagaaact
      421 tcaaaggata ctgaagaaaa gaaaacaaca attattgagg aaaaagaagt tgttagtaaa
      481 aatcctgtga tagacaataa cactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatctcaag gagataatgc acactcgtct gcaaataaaa acacagaaaa tcccaaaaaa
      601 gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccagtcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttagggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag ccaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgagg atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacactgatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccagatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgca gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctattcgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcat gctgatgaaa aggggatgat ttcctattat
     4201 ctagtagatc ctgacaaaga cgcttctaaa tatcaaaagc ttggtgaaat ttctgaagat
     4261 aaactcaaaa atgcaaagag cccagaggaa aataccaata ataatcaagc taaggatgaa
     4321 gattcaaaac cagatgaaga aagttcagtt gagggagaag ctagtcttga aataaataaa
     4381 actatttcaa caattagaga gtttgaaaat aaagacctaa agaaactcat taaaaagaaa
     4441 tataaggaag aagacgactt tgtaactggt ggaaaaagaa cagtagaact tgattataaa
     4501 tatgacgata aaggaaatat aactgcctat gaagatgaaa gtgctctaga atatgaaact
     4561 gaaaaatttg acgaaataaa atcaaaaatt tatggtgttc taagcccatc taaagatgga
     4621 cactttgaaa ttcttggaaa gataagcaat gtttctaaaa atgccaaggt atattatgga
     4681 aatagctata aatcgataga aatcaaagcg actaagtatg attcacattc aaaaacgatg
     4741 atatttgatt tatacgctaa tattaatgat attgtagatg gattagcttt tgctggagat
     4801 atgcggtttt ttgttaaaga tgatgatcga ataaaagctg aaactaaaat tagaatgcct
     4861 gaaaaaaata aggaaactaa agcagaatat ccctatgtat caagttatgg gaatgtaatc
     4921 gaattaggag aaggagacct ttcaaaaaac aaaccagaca atttaactaa aatggaatct
     4981 ggtaaaatct attctgattc agaaaaacaa caatatctgt taaaggataa catcattcta
     5041 agaaaaggct atgcactaaa agtgactacc tataatcctg gaaaaacgga tatgttagaa
     5101 ggaaatggag tctatagcaa ggaagatata gcaaaaatac aaaaggccaa tcctaatcta
     5161 agagtccttt cagaaacaac aatttatgct gatagtagaa atgttgaaga tggaagaagt
     5221 acccaagctg tattaatgtc ggctttggac ggctttaaca ttataaggta tcaagtgttt
     5281 acatttaaaa tgaacgataa aggggaagct atcgataaag acggaaatct tgtgacagat
     5341 tcttctaaac ttgtattatt tggtaaggat gataaagaat acactggaga ggataagtcc
     5401 aatgtagaag ctataaaaga agatggctcc atgttattta ttgataccaa accagtaaac
     5461 ctttcaatgg ataagaacta ctttaatcca tctaaatcta ataaaattta tgtacgaaat
     5521 ccagaatttt atttaagagg taagatttct gataagggtg gttttaactg ggaattgaga
     5581 gttaatgaat cggttgtaga taattattta atctacggag atttacacat tgataacact
     5641 agagatttta atattaagct gaatgttaaa gacggtgaca tcatggactg gggaatgaaa
     5701 gactataaag caaacggatt tccagataag gtaacagata tggatggaaa tgtttatctt
     5761 caaactggct atagcgattt gaatgctaaa gcagttggag tccactatca gtttttatat
     5821 gataatgtta aacccgaagt aaacattgat cctaagggaa atactagtat cgaatatgct
     5881 gatggaaaat ctgtagtctt taacatcaat gataaaagaa ataatggatt cgatggtgag
     5941 attcaagaac aacatattta tgtaaatgga aaagaatata catcatttga tgatattaaa
     6001 caaataacag acaagacact aaacattaag attgttgtaa aagattttgc aagaaataca
     6061 accgtaaaag aattcatttt aaataaagat acgggagagg taagtgaatt aaaacctcat
     6121 agggtaactg tgaccattca aaatggaaaa gaaatgagtt caacgatagt gtcggaagaa
     6181 gattttattt tacctgttta taagggtgaa ttagaaaaag gataccaatt tgatggttgg
     6241 gaaatttctg gtttcgaagg taaaaaagac gctggctatg ttattaatct atcaaaagat
     6301 acctttataa aacctgtatt caagaaaata gaggagaaaa aggaggaaga aaataaacct
     6361 acttttgatg tatcgaaaaa gaaagataac ccacaagtaa accatagtca attaaatgaa
     6421 agtcacagaa aagaggattt acaaagagaa gatcattcac aaaaatctga ttcaactaag
     6481 gatgttacag ctacagttct tgataaaaac aatatcagta gtaaatcaac tactaacaat
     6541 cctaataagt tgccaaaaac tggaacagca agcggagccc agacactatt agctgccgga
     6601 ataatgttta tagtaggaat ttttcttgga ttgaagaaaa aaaatcaaga ttaagataaa
     6661 agctatagaa aaaaatggtt tatgtactga gattagatag tgaggtgatg acatagtttt
     6721 gtgaaaatag ccatttataa ctcaattatt tagtttactt tactttacta gtgatactat
     6781 ttggagttat taatggactt agtttatata actaatgaat tgattgaaag ggttagtatt
     6841 gacaatattg gtcatattga ctagaaaata gagtctatca aaatttaaag gctaatagag
     6901 gtgatgagac aatttcggct ctttgtcaac tgtagtgggt tgaagtcagc taagctcgag
     6961 aaaggacaaa ttttgtcctt tcttttttga t
//
LOCUS       DAVBDG010000081         6936 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBDG010000081 DAVBDG010000000
VERSION     DAVBDG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.082"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(291..1433)
                     /locus_tag="VQ526_001841"
     CDS             complement(291..1433)
                     /locus_tag="VQ526_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2555530.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPRADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(1599..2219)
                     /locus_tag="VQ526_001842"
     CDS             complement(1599..2219)
                     /locus_tag="VQ526_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2555531.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(2223..3503)
                     /locus_tag="VQ526_001843"
     CDS             complement(2223..3503)
                     /locus_tag="VQ526_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2555532.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVAMA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(3564..5048)
                     /locus_tag="VQ526_001844"
     CDS             complement(3564..5048)
                     /locus_tag="VQ526_001844"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2555533.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(5124..6401)
                     /locus_tag="VQ526_001845"
     CDS             complement(5124..6401)
                     /locus_tag="VQ526_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2555534.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(6508..6591)
                     /locus_tag="VQ526_001846"
     tRNA            complement(6508..6591)
                     /locus_tag="VQ526_001846"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6555..6557),aa:Leu,seq:caa)
     gene            complement(6601..6672)
                     /locus_tag="VQ526_001847"
     tRNA            complement(6601..6672)
                     /locus_tag="VQ526_001847"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6638..6640),aa:Gln,seq:ttg)
     gene            complement(6686..6758)
                     /locus_tag="VQ526_001848"
     tRNA            complement(6686..6758)
                     /locus_tag="VQ526_001848"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6723..6725),aa:His,seq:gtg)
     gene            complement(6773..6843)
                     /locus_tag="VQ526_001849"
     tRNA            complement(6773..6843)
                     /locus_tag="VQ526_001849"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6809..6811),aa:Trp,seq:cca)
     gene            complement(6848..6928)
                     /locus_tag="VQ526_001850"
     tRNA            complement(6848..6928)
                     /locus_tag="VQ526_001850"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6892..6894),aa:Tyr,seq:gta)
BASE COUNT         2100 a         1625 c         1229 g         1982 t
ORIGIN      
        1 gtgtactatt ctagtttcaa tctactataa aatagattgc aagaatgttg cttagattat
       61 tacaagatga taactttcca ttttccattc aaattgatac tcaatgaaaa tcaaaaagca
      121 aactaggaag ctagtcgcag gttgctcaaa gcacagcttt gaggtttcag ataaaactga
      181 cgtggtttga agagattttc gaagagtatg acaaagaaaa agcgaacgag aatcgcttcc
      241 catcacgaaa gctagatctc attcgtcaaa atgatgttac caagtcgggt ttatccattc
      301 attgaaacgt gaacgtgatc atagtgattt tctgtcacac taccacggtc tggcattggg
      361 ttccaagtgt tagctggccc atatttgcta tcgaatggag catagaaacg ttgtttccag
      421 atgatgtaac taatgccacg gctggccatg ttttgaatag catattccgc aatcttatcc
      481 cctaattctg aacgttctgg taccataaag tcgatagcca aaccttttcc gtgatctcca
      541 ctgtctcctg gacgataacc gctaaaggat gtaatgccaa acaagttagc aatttcttct
      601 ttaaaggcag ctgtttgtgg ttgaagacct gcattttcag attttgctac tgcaagtcca
      661 gcataatcgg gcgcagctgg agcctcgtaa gttgttgaaa ttattttcgt ctcttctggt
      721 tgataagtag aaactgctgc tgtagctcca tttgatgatg ctacttcttt tgcttctgaa
      781 cttgttgtag ttgcttccac tgctgatgca gcttgagggc ttgcttgtgt ttcctgcttc
      841 tcagctggag tcgttccctg aggagcttct tctgcaactg cactgcttgt ttcggccgtt
      901 tgctcctctg ggacagaagt gcccgtagat ggtgccactt cttcagaagc aatcactgtc
      961 tttgtaactt ctgaacttga tgctacttct tttggagctt ctgcaattgg ttgagaaagg
     1021 tctgcaacct gaacagtttg atcatcaacg gtcacttgat tagtggtcaa atctgctgtc
     1081 gcagttgtca cttcttcact agagtctgct cgaggtgttt ggatttcaac ttctgttact
     1141 tcttctgctt cattgacagt cgttgtcaaa acagtttctg ggaaaatcaa gtccatatta
     1201 gtgattttgt tcagattcgc aagcactgtg acatctacac ccaaggcttc tgcaatggtg
     1261 ctcaaagtat caccatactg tacggtataa cttgttttgt tgtccgtttt agtcaaatcg
     1321 ttttggattt gctcaacact acgtgcagtc caaagaactt cttctgcttg agttgccaat
     1381 actggggcaa atgacaaggc tactgttgac gctaataaca ttcttttctt cattccttca
     1441 aattcctttc aaatgagtac ctgtctatca taacacaaaa aatgtagaaa aaaagccctc
     1501 tcgggcctat ttaacagaac tgtctattcc ttgtaacaaa ctagattact tgaaatagtt
     1561 tgttaggtct ctgtcacaaa actgacacaa tctttttatc acttagtctc aaaaatacgg
     1621 gaaatatctg ctaacgcttg aatcctgtga ttcccttcat aagtagactc taaaaagttg
     1681 atactttgaa tcccactatt ctgggcaaat tccacatcca gagtccgatc ccctatataa
     1741 taagtattat cagaattcaa ctgatactta tctagcagat aggtagccgc ttctggactt
     1801 ggcttccgca caaagccact ctgactggtt aaaatctctg taaaatagga ttccaccccc
     1861 aagtccttga gaatggtaaa agcgttgttc cccttatgag tatatataaa ctgctgaatt
     1921 cctgattcgt ctgcccaagc tagcacctca cgcgcacctg gcatcaaaac tacctgagca
     1981 ttcttctcag ccagactctg ggcacgcacc tgatttagca cctcaacatc cagatttcta
     2041 tcttctgcca cccgcacaag caaattttgc accgaatact tgaagataaa ctctctcacc
     2101 ttctccttat cataaggaat agaaaactga gcaaaagtct cctcaatccc tgataaaatc
     2161 gcttcgtaag agtccaataa agtcccgtct aaatcccaaa taaaagctgt tttttgcatt
     2221 tcctatcctt tcaagctcat atagcgctgg taacgatagc gtagaaataa ccagcgaaaa
     2281 ccattatcca agagagaccc tgcccaaata ccaggcaagc cccaaccaag cacaatcccc
     2341 atcagatatc ctgtcccaat gcggatacac cacatgccta tacttgtcgc ataaaaaggg
     2401 aggcgtgcat ttcctaatcc ctgccagact gccgtataga tgactgttcc tgtcgtcata
     2461 ggggtaccaa gtagtgaaaa cagtgtcact agaacactag cctccaccgc tagagaatca
     2521 gtcgtataga gatgagttaa tggtacaccc aagacatata tactaaagga caggggcaac
     2581 atgaggaaca gagaaagcca aaaggtttgt ttactcaaac tagcaactct tttccaatca
     2641 tcctctccaa ctgctcgggc caacagcatg accgttgcca tagcgacacc aaaggcaggc
     2701 atatagttaa actgggtcaa gacttctccg attgcattcc cagcaactgc ctccgtccca
     2761 aaagaaacga ccaaggcaat gatcactaca tctccagctc tcatcataag tcgctctcca
     2821 gctgctggta aagccaaggt caacagttcc ttatctaaac caaaagttag cttcccataa
     2881 ggcagtttta attgtgacca caaaatcaca agaccaacca aacgagacag aattgtcccc
     2941 caagcaacac cagctatccc catatccaga acaaaaatag ctagacttga aaaaagaata
     3001 ttcaaggcat tggataaaaa actaacatag agaggcagac gtggattatg cgttgcacga
     3061 atcaaggctc ccaaactagt cattaaacct aagagaacaa tcgatccgcc taccaaagat
     3121 agatacagtc caccactctc agctacatcc ctctccgtcc ccaaaagtcc tatcatctct
     3181 ttcccagcga agatggacaa aaatcctaaa aggaaactta atagtaaggt aatcttcaac
     3241 gcctcagtca catgataggc caacttcgac tggtctttct gccctatgct ttttgaaata
     3301 acactggaaa tagcagctcc cagagcgatg aaaatcgcct gataaatggt gataatatta
     3361 ccagctactg aaacccctga aatagctatc aatcctaaat gagcaaccaa ataactgtcc
     3421 accattccca ttagcatctg caaaaagttt tcacccatag ctggcaatgc aatattaaga
     3481 atgtctttat ttttcttaaa caatctctcc tcctgatgaa aagaaactca gttggtttcc
     3541 caaccgagtt tgctccctct gtcttaaagt cctaaataag cctcaaccgc tgcttgcatg
     3601 tcagcagctg ccactgttgt cttgtgacga attggagcta tttcaagccc atcaactgct
     3661 ggtggcactg caacgcctga gatttcatgt aattgagcca aggcttcaaa gtctgtcaaa
     3721 cctgcttttc cagttacagc ttctactgca actactggga acttgtatgg actagctgtt
     3781 gaagcaatca ctgtcttagt tacatctcca gtaaccgctt ggtattttct ataaactgct
     3841 gaggcaaccg ccgtatgtgg atcctcaata taagaatctg actcataaac acgcttgatt
     3901 tctgccgctg tttcttcctc agtcgcatat tcagctgcaa agaggtccaa aatctctgca
     3961 tcaaagtctg tcaacttata ttgtccttgc gtgttcaagg cattcataag ttcagctgtc
     4021 ttttcagcat tatttcccaa aagatggaaa atcaagcgct ccaagtttga agataccaag
     4081 atatccatag atgggctggt tgttacctta aactcacgtt ttttgtcata gacacgtgtt
     4141 ttaaagaagt ctgtcaagac attattgtca tttgaagcac agattagctt gccaactggt
     4201 aggccgattt gtttggcata aaaggcagcc aagatatttc caaagtttcc tgttggtact
     4261 gtgaagttaa ccttttcacc agctacaatt tcaccagtct taaccaattg agcgtaagca
     4321 taaacataat aaacaatttg tggcaccaga cgaccaatgt tcatagagtt agctgatgaa
     4381 aattgcaact tgttggtagt caatttttca cgaagagcca cgtcgttaaa catgtgcttc
     4441 acatttgttt gcgcatcgtc aaaattacca tcaatagcaa taacatgagt attgtcacca
     4501 ttctgagtgg tcatttgcaa ctcttgtacc ttgctgacac caccctttgg ataaaagacg
     4561 ataatctcag taccaggcac atccgcaaac cccgccatag cagctttccc cgtgtcacca
     4621 gatgtcgctg tcaagataac aatcttgttc tccaaaccat gtttcttagc agcagtcgtc
     4681 ataaagtatg gcaaaataga caaggccata tccttaaagg caatcgttga accatggaaa
     4741 agttccaaat tgtattgccc atctaatttc actaatggtg caatagctgg agtatcaaat
     4801 ttgctatcgt aggcattgtt gatacagtag tccaactcct cagctgtaaa gtcatctaaa
     4861 aatgctgaca aaactagctt agcaacttcc tggtaagaag catctttcaa tttgtcaaag
     4921 tccaaatcta cctttggata agtaaccggt gtaaacaaac cgccgtccgt cgccaaacct
     4981 tgcaaaattg cttggctggc agttactgta ttgttggcat cacgcgttga ttgataaact
     5041 aatgtcatta ctctctatcc tttatctata gtctcgtcca ttatatcatg atttttcaga
     5101 aaattctccc tttataagag aaattatagg ctcaattctt ctctcaaagg ctgtaaatag
     5161 gtcatttctt ccttatttct caccactagt ccctcctcag ctagtaggag taagtctttg
     5221 gaaaattcaa taatcgtagt ttcttcctca tctgtaagat ttttcttaga aaattgtctc
     5281 cttaaagact tgtaatcata accaaatact ttaaagaaag gtgctgtttc taagtaagct
     5341 tctaacttgt ctaaattaac caataatccc aagtgaaaag ctgcagaagc aaaagtccta
     5401 tcaagtggct gtgtacacac actacgaaac tcaactgttc ctcgagtcgt taaatcttgg
     5461 tactggtaac tacgatgagt ttcaaaatcc ttctcttggg ggtaaataat aacctcatcc
     5521 ccattcagag caaatgcttg gatttcggac gtagccaaat agtccccagc ctgaatagga
     5581 taaaaataat aggtctgccc atcacgttcc gcagtaaaaa tcgcagaatg atttagatag
     5641 tcaaaaaaat cagcttcatc attaaggagt ctagcattga ccccaacatt ctctggatag
     5701 ataccatgca tagattcttc ccagaaaata tcccttgaaa ttttcgtatc ccaatccgca
     5761 cccgaaaatt cagagtttgc aaataaataa gccttagccg cttcaatttg agtaaaagca
     5821 ttaatcaccc gtaagtagtt ggtttttgaa atatccagct gaacctggct cccacagata
     5881 aaagtaccat attcagggaa atgatgtaaa tctgatttaa taatatttct actcaaattc
     5941 aaataatcca tcaacatctg atagcgtgga taagccactg gacaattctc atttttatcc
     6001 cagttgggat ggataccaca gccaacaata gcatgatttg attcagctaa ctttctctga
     6061 attacattca tataattatt gaaacgattt tcgacctctt gaatcgtttc agccttacca
     6121 aatgcaaact caatcgttgt ataggaaact tcaaataaaa tagcatcctg acttatcgga
     6181 tctactaact ggatcagatt gccaaaatca tctacctttg ctacggtgag atccaaagta
     6241 gaaactaaat aatgaaatag atcctttata acttcaacat ctgtagcatc cccttctaaa
     6301 cttgcaacag gatactccaa ctcaattccg acaaacaatt caggattttc tttaatattt
     6361 ttcaaatacc gatgttttaa taaatcgata gaacaagaca tactacccta cactttctta
     6421 atctaaataa aatttgaata actataatta taccaaaaat aatataaaaa aggaacgaag
     6481 aataacaacg ttcctaacac tatactatac cggcggccgg ggtcgaaccg gcacgtcctt
     6541 gcggacactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caaatagtaa
     6601 ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt
     6661 ggctataccc caataatata aaataggcga gtgatgggga tcgaacccac gcatgccaga
     6721 gccacaatct ggtgtgttaa ccacttcacc acacccgcca taattctatt aacacgggca
     6781 gtaggaattg aacccacact gaaggttttg gagaccttag ttctaccttt aaactatgcc
     6841 cgtaaaatgg aaggggaggg attcgaaccc ccgaacccga aggagcggat ttacagtccg
     6901 ccgcgtttag cctcttcgct acccttccaa aatata
//
LOCUS       DAVBDG010000082         6928 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBDG010000082 DAVBDG010000000
VERSION     DAVBDG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.083"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..474
                     /locus_tag="VQ526_001851"
     CDS             <1..474
                     /locus_tag="VQ526_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET2555535.1"
                     /translation="KMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEIQ
                     LALIATKPKLSYLSTLIRYEELYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIF
                     ERIQIYQRDRGCVYDAKENTTSAADVLQELLFGEWSQVEKDMLKSGEERLKDLTN"
     gene            950..1738
                     /locus_tag="VQ526_001852"
     CDS             950..1738
                     /locus_tag="VQ526_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555536.1"
                     /translation="MTTTKNPWNQLSNVDINGEQAILATEDVELIKKYTSTKHYKNLK
                     DDIYRLQLGFCPQQFVGDIQNADIIVLSKNPGYTPEFKTLYDHDKNYQKTLLNNLQLK
                     GNLYFHAFDLDTNEFGYWAKKFKVWFDDVDNLQDLKEKLPWFSKHVALAEYFPYYSTK
                     YDDKLNDFISKEGYFPTQKFLFNLIRERVLDDNDPVIIIITRSYNKWYDAIPQLKEYK
                     NCYETSNPSNPSLKPENLLKVKRYSAKKEVEKVLEDSLKKLEHK"
     gene            1977..2225
                     /locus_tag="VQ526_001853"
     CDS             1977..2225
                     /locus_tag="VQ526_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555537.1"
                     /translation="MNYQKLNDITGATKNEKDKYYVYGLYEEGKQLPFYIGKGEGTRL
                     ISHIDEALTEINQEENIQISKKIQIIRKHKGKIIPVII"
     gene            2396..3115
                     /gene="catA"
                     /locus_tag="VQ526_001854"
                     /pseudo
     CDS             2396..3115
                     /gene="catA"
                     /locus_tag="VQ526_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
     gene            complement(3911..4834)
                     /locus_tag="VQ526_001855"
     CDS             complement(3911..4834)
                     /locus_tag="VQ526_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HET2555538.1"
                     /translation="MEKYTEKKQRNQVFQKFIKRHIGENQMDLVEDCNTFLSFVADKT
                     LEKQKLYKANSCKNRFCPVCAWRKARKDALGLSLMMQYIKQQEKKEFIFLTLTTPNVM
                     SDELENEIKRYNNSFRKLIKRKKVGSVIKGYVRKLEITYNKKRDDYNPHFHVLIAVNK
                     SYFTDKRYYISQQEWLDLWRDVTGISEITQVQVQKIRQNNNKELYEMAKYSGKDSDYL
                     INQKVFDAFYKSLKGKQVLVYSGLFKEAKKKLKNGDLDYLKEIDPTEYIYQIFYIWKQ
                     KEYLASELYDLTEQEKREINHKMIDEIEEEQ"
     gene            5038..5868
                     /locus_tag="VQ526_001856"
                     /pseudo
     CDS             5038..5868
                     /locus_tag="VQ526_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            6136..6591
                     /locus_tag="VQ526_001857"
     CDS             6136..6591
                     /locus_tag="VQ526_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555539.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGNSHVNNRQEHYNRGSGNLQP
                     EEWNKTAKQLYNAIMWLIILWDAKPDTPLFDFKDEVIKYKTYEPFDIKIKRVNTTIKN
                     GSQGKTLTEMINGYRADNDIRDEICNFDLLKNKIRDMKDQQGNTMESYF"
BASE COUNT         2468 a         1051 c         1085 g         2324 t
ORIGIN      
        1 aaaatggtag agtctttagt aagagaattg agtcatttgg gatacaatct tttgatagag
       61 ggaactttac gaactattga tgttccaaag aaaacagcac aactcttgaa aagtagggga
      121 tatgaaatac aattagcctt gattgcgaca aagcctaagc tgtcctatct gagcaccctt
      181 atccgatacg aagaactgta cgctattaac ccaaatcaag cacgcgcaac tccaaaagaa
      241 catcatgatt tcattgtaaa tcatctagtt gataatacac gacaattgga agaactagct
      301 atctttgaaa gaattcaaat ttaccaacga gatagaggtt gtgtatatga tgcaaaagaa
      361 aatacaactt cagcggcaga tgttcttcaa gagttactgt ttggggagtg gagtcaagta
      421 gagaaggata tgcttaaatc tggagaagaa agattgaaag atttaactaa ttgaaatggt
      481 tgttagatct tgagttttaa aataaagcct attaaaaatt gctatctctt agattatggt
      541 aaaaaaatgg cttttaatta aactaacaaa gtgagtataa ataaaagtaa taaatctcac
      601 cgtcattctt ttttaaatta atcgttgttc tcaaaatatt tccaatttat cggacttttt
      661 gtactagtct tatacttttt agttaattct atctggccta tagtattgaa aataatttgt
      721 agacttggca atgagaattc gttacagttt agtagagccg acgtattccg aacatcattt
      781 acggcggtgt gaaaggtgat ataaataata ttatatctat tgatattcaa aatcgtttcc
      841 tcactcagat tcttttataa tcgagaatat attaataaaa tctatttttg tatttaatat
      901 taaaacatcg taaaatatag taattctaca aaaagaaagg aaaaataaaa tgactacaac
      961 taaaaatccc tggaatcaat tatctaacgt cgatataaat ggagagcaag ctattcttgc
     1021 taccgaagat gttgaactaa tcaaaaaata cacctctaca aaacactaca aaaatctaaa
     1081 agatgatatc taccgtttac aattaggttt ttgtccgcaa caatttgtgg gggatattca
     1141 aaatgctgat atcattgttt tatctaaaaa tcctggctac acccctgaat ttaaaacact
     1201 ttatgaccac gataaaaact accaaaagac tcttttaaat aatctacagc taaaaggaaa
     1261 cctctatttt catgcttttg acctagatac gaatgaattt ggttactggg ctaaaaagtt
     1321 taaagtatgg tttgatgatg tagacaattt acaggattta aaagaaaagc ttccttggtt
     1381 ttcaaaacac gttgctttag ctgaatattt tccatattac tctactaagt acgatgataa
     1441 attaaatgat tttataagca aagaaggtta tttcccaaca caaaagtttt tattcaattt
     1501 aattagagaa cgtgttttgg atgacaatga tcctgtaatc attatcatca cacgatctta
     1561 caataagtgg tacgatgcaa ttcctcaatt aaaagagtat aaaaattgtt atgaaacgag
     1621 taatcctagt aatccttctt taaaacctga gaatttatta aaagtcaaac gatattctgc
     1681 taaaaaagag gttgaaaaag tattagaaga ttcattgaaa aagctagagc ataaataaaa
     1741 cagtctcttt ttaatgctat cgtaaatact gtattgtgta gttattagta aacctatata
     1801 ttgtccccaa atttatttag attattctct aggattttac ttgattttaa agaaaatata
     1861 gtttaacata taaatagttc agaatcagaa tgaaggtaga ataagaggat tattttaatt
     1921 aatttttaat agtatggagc agaactttta taatatattt aaataggagt aaaaaaatga
     1981 attatcaaaa attaaatgat attactggtg ctaccaaaaa tgaaaaagat aaatactatg
     2041 tttacggact ctatgaagaa ggtaagcaac tacctttcta tattggtaaa ggagagggaa
     2101 ctcgcctaat atcacatatc gatgaagcgc ttacagagat aaatcaagaa gaaaacatac
     2161 aaattagtaa aaaaattcaa attattagaa aacataaggg caaaattata cctgtaatca
     2221 tttaaagcac ccattagttc aacaaacgaa aattggataa agtgggatat ttttaaaata
     2281 tatatattta tgttacagta atattgactt ttaaaaaagg attgattcta atgaagaaag
     2341 cagacaagta agcctcctaa attcacttta gataaaaatt taggaggcat atcaaatgaa
     2401 ctttaataaa attgatttag acaattggaa gagaaaagag atatttaatc attatttgaa
     2461 ccaacaaacg acttttagta taaccacaga aattgatatt agtgttttat accgaaacat
     2521 aaaacaagaa ggatataaat tttaccctgc atttattttc ttagtgacaa gggtgataaa
     2581 ctcaaataca gcttttagaa ctggttacaa tagcgacgga gagttaggtt attgggataa
     2641 gttagagcca ctttatacaa tttttgatgg tgtatctaaa acattctctg gtatttggac
     2701 tcctgtaaag aatgacttca aagagtttta tgatttatac ctttctgatg tagagaaata
     2761 taatggttcg gggaaattgt ttcccaaaac acctatacct gaaaatgctt tttctctttc
     2821 tattattcca tggacttcat ttactgggtt taacttaaat atcaataata atagtaatta
     2881 ccttctaccc attattacag caggaaaatt cattaataaa ggtaattcaa tatatttacc
     2941 gctatcttta caggtacatc attctgtttg tgatggttat catgcaggat tgtttatgaa
     3001 ctctattcag gaattgtcag ataggcctaa tgactggctt ttataatatg agataatgcc
     3061 gactgtactt tttacagtcg gttttctaat gtcactaacc tgccccgtta gttgaagaag
     3121 gtttttatat tacagctcca gatccatatc cttctttttc tgaaccgact tctccttttt
     3181 cgcttcttta ttccaattgc tttattgacg ttgagcctcg gaacccttaa caatcccaaa
     3241 acttgtcgaa tggtcggctt aataactcac gctatgccga cattcgtctg caagtttagt
     3301 taagggttct tctcaacgca caataaattt tctcggcata aatgcgtggt ctaattttta
     3361 tttttaataa ccttgatagc aaaaaatgcc attccaatac aaaaccacat acctataatc
     3421 gataaccaca taacagtcat aaaaccactc ctttttaaca aactttatca caagaaatat
     3481 ttaaatttta aatgccttta ttttgaattt taaggggcat tttaaagatt taggggtaaa
     3541 tcatatagtt ttatgcctaa aaacctacag aagcttttaa aaagcaaata tgagccaaat
     3601 aaatatattc taattctaca aacaaaaatt tgagcaaatt cagtgtcgat tttttaagac
     3661 actgcccagt tacatgcaaa ttaaaatttt catgattttt tatagttcct aacagggtta
     3721 aaatttgtat aacgaaagta taatgtttat ataacgttag tataataaag cattttaaca
     3781 ttatactttt gataatcgtt tatcgtcgtc atcacaataa cttttaaaat actcgtgcat
     3841 aattcaacag ctgacctccc aataactaca tggtgttatc gggaggtcag ctgttagcac
     3901 ttatattttg ttattgttct tcctcgattt cgtctatcat tttgtgatta atttctcttt
     3961 tttcttgttc tgttaagtca taaagttcac tagctaaata ctctttttgt ttccaaatat
     4021 aaaaaatttg atagatatat tcggttggat caatttcttt taagtaatct aaatccccat
     4081 tttttaattt ctttttagcc tctttaaata atcctgaata aactaatacc tgtttacctt
     4141 taagtgattt ataaaatgca tcaaagactt tttgatttat taaataatca ctatctttac
     4201 cagaatactt agccatttca tataattctt tattattatt ttgtcttatt ttttgaactt
     4261 gaacttgtgt tatttctgaa atgcccgtta catcacgcca taaatctaac cattcttgtt
     4321 ggctaatata atatctttta tctgtgaaat acgatttatt tactgcaatt aacacatgaa
     4381 aatgaggatt ataatcatct ctttttttat tatatgtaat ctctaactta cgaacatatc
     4441 cctttataac actacctact ttttttctct ttataagttt tctaaaagaa ttattataac
     4501 gttttatttc attttctaat tcatcactca ttacattagg tgtagtcaaa gttaaaaaga
     4561 taaactcctt tttctcttgc tgcttaatat attgcatcat caaagataaa cccaatgcat
     4621 cttttctagc ttttctccaa gcacagacag gacaaaatcg atttttacaa gaattagctt
     4681 tatataattt ctgtttttct aaagttttat cagctacaaa agacagaaat gtattgcaat
     4741 cttcaactaa atccatttga ttctctccaa tatgacgttt aataaatttc tgaaatactt
     4801 gatttctttg ttttttctca gtatactttt ccatgttata acacataaaa acaacttagt
     4861 tttcacaaac tatgacaata aaaaaagttg ctttttcccc tttctatgta tgttttttac
     4921 tagtcattta aaacgataca ttaataggta cgaaaaagca actttttttg cgcttaaaac
     4981 cagtcatacc aataacttaa gggtaactag cctcgccggc aatagttacc cctattatta
     5041 aatacagcta atccagaggt caaggattat cttttaaagg ttgcgactta ttggattgaa
     5101 gagtttgata tcgatgcttg gcgtttggat gtggctaatg agattgacca tcagttttgg
     5161 agggattttc gcaagacagt tctagctaaa aagcctgatc tttatatttt aggagaagtc
     5221 tggcatacgt ctcagccttg gctaaatgga gatgagttcc acgcggtcat gaattatcct
     5281 ttatctgata gcatcaagga ctatttctta cgaggagtta agaagacaga ccagttcatc
     5341 gatgaaatca atgaccaatc tatgtattac aagcagcaga tttcagaagt tatgtttaat
     5401 ctcttggatt cgcatgatac agagcgaatc ttgtggacgg ccaatgagga tgttcaactg
     5461 gtcaaatcag ccttagcctt tctctttcta caaaaaggaa caccgtgtat ctattacgga
     5521 acagagttat ccttaactgg aggtccagat ccagattgcc gtcgttgtat gccttgggaa
     5581 cgcgtctcaa gtgataatga tatgctgaac tttatgaaga agctgattga gattcggaaa
     5641 cccgcgtcag caatcattca gcatggcaag tatagtctcc aagaaattaa acaagatgta
     5701 gtagcattgg aatggaaatg cgatggacaa attcttaaag ctatctttaa ccagtccaaa
     5761 gaagattatc ttttagagaa ggaaacagta gcactagcaa gcaattgcca agaattagag
     5821 aatcagcttg tcatctctcc aaaaggattt gtgattttca aaaactagtt gatgaaggtt
     5881 atgatttttt actcaacttg tatagtataa taaggctagt tactaaactt gtaaaggaga
     5941 acttaaatga attgtagagg acatgaaaca agacaaagaa ttgttagaga ttttgaagtt
     6001 cagcctaaag cacatattaa gctgttagca aatcaacaaa aacatagtga tgcaggagca
     6061 actattgaag atgaatatta tgtatttatc gctgagagta aaattgatgg caagaaggaa
     6121 gttattcagt gtggcatggg tgcggcaagg gattttttag aactaattaa tcacaaaggg
     6181 ctacctcttt ttaatccgct tgtaggtaat tctcatgtaa ataatagaca agaacattac
     6241 aatagaggga gtggaaattt acaacctgaa gagtggaata aaactgcaaa acagctttat
     6301 aatgcgataa tgtggttgat tatcttatgg gatgctaaac cggatacacc tttatttgat
     6361 tttaaagacg aagtaattaa gtataaaaca tatgagcctt ttgatatcaa gataaaaaga
     6421 gtgaatacca ctataaagaa tggtagtcaa gggaaaactc taactgagat gattaatggc
     6481 tacagagcgg ataacgatat tagagatgaa atttgtaact ttgatcttct gaaaaataaa
     6541 attcgtgata tgaaagatca acaaggaaat acaatggaat cttactttta gttattgttg
     6601 aattttgggt attctataaa atatcccaat tgagatttaa atagtagact atacaatata
     6661 gttaaaatat cagtaaaaac aacactttat tgagatgttg gatacgcttt actaatgacc
     6721 taataataca agtataaata tttatggctc tttaggtggc ttttttagaa gtaaagttta
     6781 ggctagaaaa aaagtataac gcaccaaaat tggtgcgtta tactttttta tgctataatt
     6841 gaattataaa aataaaggag tttgccatga ttggaaagaa cataaaatcc ttacgtaaaa
     6901 cacatgactt aacacaaccc gaatttgc
//
LOCUS       DAVBDG010000083         6806 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBDG010000083 DAVBDG010000000
VERSION     DAVBDG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.084"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..207)
                     /locus_tag="VQ526_001858"
     CDS             complement(<1..207)
                     /locus_tag="VQ526_001858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555540.1"
                     /translation="MENMTNHLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFF"
     gene            complement(275..916)
                     /locus_tag="VQ526_001859"
     CDS             complement(275..916)
                     /locus_tag="VQ526_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555541.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(918..2947)
                     /locus_tag="VQ526_001860"
                     /pseudo
     CDS             complement(918..2947)
                     /locus_tag="VQ526_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(3216..4115)
                     /locus_tag="VQ526_001861"
     CDS             complement(3216..4115)
                     /locus_tag="VQ526_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2555542.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(4187..4747)
                     /gene="rnmV"
                     /locus_tag="VQ526_001862"
     CDS             complement(4187..4747)
                     /gene="rnmV"
                     /locus_tag="VQ526_001862"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2555543.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAHVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(4747..5520)
                     /locus_tag="VQ526_001863"
     CDS             complement(4747..5520)
                     /locus_tag="VQ526_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2555544.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKNLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAAATTANAERIFGLDSK"
     gene            complement(5825..6718)
                     /locus_tag="VQ526_001864"
     CDS             complement(5825..6718)
                     /locus_tag="VQ526_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2555545.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLETTPGGDKGGNTGRSDANANAGGGSQAGGPVHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVAVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
BASE COUNT         1907 a         1620 c         1175 g         2104 t
ORIGIN      
        1 aaaaaaatca gcttcttgat aacttcgatt ttcgggtagg gtcaaatcca catcctcctc
       61 cacatcatcc actgtcagtt caaccgtttg aggtaaattt ttcaaacgat aatgcttttg
      121 cctttgccct tcactactag caaaatcaag caaatagcca tgcactcgga tttgaaccaa
      181 aaggagatga ttggtcatat tttccatgat gaggatatct tataaaccac ctctggcact
      241 aggataatca gataccctgc gaatacggat tttttcacac atgagcaagt ttcctcatat
      301 caatgatttc atcagcctta ttccagacta ggggattatg ggtcgcaatg atgataattc
      361 gattttcatc tttcaaatcc accaagagat tcataacctc ctctgaattt tcaggatcaa
      421 gagctgctgt tggttcatct gccaaaatca agggtggatt tttcaaaata gttttcgcaa
      481 gggcaactcg ttgggcctct cccccagata aagtataaat tttttgttct aaatccaaat
      541 accctagatt aactttttct aaagccccca cttgcctttc caaacgttct acttttgaga
      601 ttttctgacc aacaaaaccc aaatccaaat tttctttgat tgattggttt tctaagaggc
      661 caaaattttg aaagagatag cccatctgat ctcgaaaata ctcacgagtg ggaatggttt
      721 ttaaatcttt cccctgatag agaacccttc ctccatctat cttttctagc tttcccaaga
      781 tattcagcag cgtcgtcttt ccgcttccac tctttccgat taaggcataa accttgccct
      841 tctccagctt gagattcaaa ccagagaaaa tcgcgcggtc attaaatttc ttttccaatc
      901 cttgaatatc aatcatctta ttttcctttc aatactgcca tgctaccagc ttcttctttt
      961 ttgtcctgcc ttactaaaat caagagggcg ttaagcgtaa acaaagctac aactagagcg
     1021 ctaatcaaac catctcttgt caaaatacta gataggacta ggccaagcaa gagaactcct
     1081 ccttgcgcca gtaaatactt gccatgaagc tcatagattg tcataccagc aagacgttta
     1141 atcataagct cccgtctgaa ctgctcaaaa tagagaagat tcatggaatc aaataacaag
     1201 atagccgtag acagggtcaa aatcgtccca ataaggagag agtaaaactc tacccgtttg
     1261 tcatttagta ccttggcaaa aatagctggc tttttaccaa gtaagaaacc ttgtgataca
     1321 gaccttgctc ttttagagct gtgatgctgt ctccatatcg atctagtttc aaggcactat
     1381 tagccgtagt tccccacaac atccctgcaa caggtcttgt tccagtcgct tgcggcgtta
     1441 gaactacaat aatcggatct tttaggtact ggcgtgttgt cttgtaccca tcattataga
     1501 ggaaacgttc ctgtcctgtt tctgtaaaag ctagacttac ctgtgggagg tagtatttct
     1561 gactcgtcac ttctacactt tcagatgaaa agttttgcag gtaatctgta aacaatcctt
     1621 ggtagtaggc agactgctct cgcaagctct caggcaagat cagcccaaac tctccctcag
     1681 acaagttttg catcttgtcc ataaactctg aagaaacggt aatcttttct tctatcagat
     1741 agcgtggtga gacatagata acattccctg acggtgtgta gtcactgaga cgattgccat
     1801 ctaggtccac ttctgctccg tctgagaaat tgtcaacatt gctcataata taaaaagagt
     1861 ctgtattggc taaccgttct tcagtaaatg tctgccactc acgattatcc ttacgcgttc
     1921 cttcttcatc ggcaaatgca ctagaccaac caaaggatag acggtaacgg tctgaggact
     1981 ggctccattt attgctagct ctctccattt cctgcatttc acggtagtgg ggtaggagag
     2041 ctgtcgcact cgatccgacc accaatacag ctaagagttg ccccaccatc atcaatgtca
     2101 tcatacgttt gagagggagt ttccctttca atagatccac cagactattt tcctgtaaac
     2161 caagtagata gacgacactt agtaaggtag aaatccctgc cagggtcaat ccataaagta
     2221 gaagagcaat gatgaccagt tgcaccgttg ccataaacaa gacaccttga taccagagaa
     2281 tccccaatcc caaaagactg gataccagca ctgagcagat aaactgtctc acatcttcta
     2341 acactggtct gagagcaact ccaaacaagc tctcaccagc tattaagcga atccctgcct
     2401 gacgaaggga tttgatccga taaatcaggg tcagactcat aaaggtcagc agaaaaatag
     2461 ctaaactcag tagcaccata ggggtggccg ccaatagtaa gactatcgaa aatggatctt
     2521 ctccattcga aacaaagcct tgatagccaa gttctttcaa ggtggtctgt aagctcactc
     2581 catccaaact tcctgacaca atcaagtaag accctaataa atcactcgtt tctgcactct
     2641 ccttggagga aatggtcaga ccttctggaa gctctccctc accataagtg gcataggtaa
     2701 agagtgtttc tccagcttca tttggctcaa cgattcgacg ggcgatgaga ctattatgct
     2761 ctaccgccaa gtgactcaaa acctctgtca cctcttcgcg actagctact ggttgattta
     2821 tcatcattcc tgtcacatca acagaaggat agttaccaaa taaaatctct tctcgatggt
     2881 caacggaagt tatcaccata taaagcgata ccaataccat tgaaatcaaa ataaataaac
     2941 gtttcattat ttcctccaaa aagttacttg gtcacttagt gagaaggtga ccacagtcac
     3001 tgcgatgtta agaagcttat taagttcttt ctactagcaa catcttcaaa tacattatat
     3061 ccgttttttc aagaactatc aaggacatca atgtccttaa aatctaacca tttcgtgtca
     3121 tttttagctt atatctaaaa aaacagccct aaagacgaac tttagaactg ttttgatgcg
     3181 agaacaagga catttttgtc actgattcac tatgtttata aatatttttt gacatcctct
     3241 tgccattcct ctgtcaattt ctttactaga aattcatctc caattagctg cgcaaatgtt
     3301 ttagaagact gataaaatga ttcagctaaa ctaaaatctc tttcttctct taatgcttgc
     3361 ttccacaaaa acataaatac aagaggtttc ttttgataat cttgagtttt atccataatt
     3421 tcttgtaaac acgatagata atcattaaaa caatcagtta ctcctacctt ttcaagacaa
     3481 cacattcctg aaataagagt atctctaatt ataaagaagt tttctaattt agttaccttt
     3541 ttctgttcca aaagaaagag cattagcttt tcgatttgat ttataaattt ggcaagatct
     3601 tttctaatga gcatttgata aaaaaacaaa cgaatcaaca acatatcatt tgttgaataa
     3661 acttcttttt ctatgatctg aggcatatgt tcctcaagta ttaactctgc atattcaggc
     3721 cttctagttc tagaagtatc caaacacgcc tgcaatactt cacatgctaa ttgttcttct
     3781 ttaggaagat tatcaaagta tgttttataa atctcttcca aacacgcttc cttcttatcg
     3841 tactcctctt ctttaccata gattggttca cgtaaaatct ggtatttcaa ttctaaataa
     3901 gcaggaggaa gagctgaaaa atccggcata aggctataaa ctggaactcc tagccggcga
     3961 gcaatatagt ctaacttggg caaactcggt tgcgaagctc cactttcaat tcgcgacagt
     4021 tgacgaactg tcagttcttg ctcatctcca caaaaatctg gacgactaat tcctttttct
     4081 atccgaatct ctttaatttt ttgaccaatt aacatcagac gcctcctctt ctacctgctt
     4141 cgaaaaatac tgtgaaactt aaaaaattgt aacggctaca tctttcctac tcataagatt
     4201 tcatagcttc ttccacttct gccaaagtaa ccccaaacaa ctctaggcgt ttgaggagtt
     4261 gcttgccgtt ggaatagccg attcggagag tctctccgag atattctcta cgcttacggc
     4321 tgtctgcccc tgctagaaaa ccaaggcgaa tcaaatcgct acgactaatg tcaaactgac
     4381 tctcatgttc aaattgttct gtcacatgag ctagagccgt tttcaggtct tcatagctgg
     4441 catgctcaat tcccagagaa cgccccttgg tcttggactt gggaacagct tcatctcgct
     4501 tgagaaaggc atgctgaact gttggaatga ccgtcatgat catgcgccga atccgttccc
     4561 cattaaaatc tgggtctgta aagacaatga ctccatgacg ttggtgcagg cgctgaatcc
     4621 gctctatatc ctgatcattg atggcagaac ctcgagtctc ataggtctcc acatcgaaat
     4681 aacgcttgag attgaccgta tcatcacgcc cttcaaccac aattacttgg gaaattttct
     4741 ctttcattac ttgctgtcca atccaaaaat gcgttctgca tttgcagtcg ttgccgccgc
     4801 tagctcttct gtcgtcatac cacgcaagtc agcgataaag tcgaccacat agcgagtata
     4861 ggctgtttta ttttcacggc cacgcttggg tacaggtgct aagtaaggcg catctgtttc
     4921 caccaacatc ttgtccaaag gtaactcttt agctgcttct tggaggtcag ttgccttctt
     4981 gaaagtcacc actcctgaga aggaaatggt cataccaaga tccacaaact tctctgccca
     5041 ctcaagcgtc cctgaaaatg aatgcatgat accaccacga ggaccaacgc cctcactctt
     5101 gataatctca taggtatctt ccagcgcatc acgggtatgg acaacaaaag gcaaatccaa
     5161 gttcttagat agctgaatct gacggcgaaa aacctgctcc tgcacctctt tgggcgctgt
     5221 catccaatgg tagtctaagc caatttcacc taaagccaca accttggaat gttttaactt
     5281 atccaacaag taagcctcaa cttcctctgt ataagtacca gcttctgtag gatgccaacc
     5341 aatagtcgca tagagctgct catactcatc taccaactcc aaggcatgct caatcgtcgg
     5401 tttatcaaaa ccaacaatat tcatctgtgt cacacccatc tcagcagcca aggcaatttc
     5461 ttctgcctca cgacctgcaa attcttctac attcaagtgt gtatgtgtat caaaaatcat
     5521 ctcttctaac ctcgttttct atcttctatt ataccaaaaa agaggagggg cacctaattt
     5581 ttcggtttcc cctcctctct tcaataaaga gctattctgc tatcttttct atccgatatt
     5641 gcccatctcc tattccacag ttagagacag aagagattgg ctacattgat agaatattcc
     5701 tattcagcta aagtatgtga ttatcttggg ttctcacttt tctcacccat cactaggatt
     5761 gatgacttct caaaatcccc cttataatct aactttaagt agagaataga attttctagc
     5821 tgttctattt tctacgtcta gtctttcttc ctagcaaagc aaaaccactt gttgccacca
     5881 ctagaccaag actagcaagt gctgcacttg atgctgaacc tgtgttaggt aattgtttgc
     5941 cagcaggtga ctggtaggta acatcttgca ttgttcctgc agttgcttgc ggtgcacttg
     6001 ttgtcttagg agcagcatta ggagccactg gtctcttagg aactgcaacc atggcaatgg
     6061 cttgcactcc aatcgtttct gcttccttca catcttccat agttttcgca ttttcaatct
     6121 ctttgagagc tgcttgtttt tctgcttcca ctcttgctaa aagttctgct ttttctttat
     6181 cagaaagcgg tgcgcctttg atttcatcct gcttgttctt ggctgctttt tcaatggcat
     6241 ttttagctga ttctttttca gtagctaatt gcttagaagc ttgtgattga gctgagtttt
     6301 gtgaacctgt gtgaactggt ccacctgcct ggctaccacc gcctgcattc gcattagcat
     6361 ctgatcttcc agtattgcct cccttatctc cacctggtgt agtttctagc ttatactctg
     6421 gtgtagcttc ttgagttaca gattcacctt tcgcagattg ttgaactgta tcttttccta
     6481 gaatcgtcac tactgaacca tctgggaatg tgattgttgc tgtaccatct ccagctacat
     6541 tgattgtcgc tccgtctaat gctgaaccat ttgcttgtaa aatagcaacc ttaacttttt
     6601 cttcatctgt taaatgagtt atatctttta catctactct ttgagcaggt gctttaacag
     6661 ttgatttttc agcagtcggt ttagtactat cttgtgcttt tggatcttgt gctaccaatt
     6721 gatttcctgg aattgtaata gctgaaccat ctttaaagac gattgtcgct gtaccatctc
     6781 cagctacttc aactttatag taaaat
//
LOCUS       DAVBDG010000084         6765 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBDG010000084 DAVBDG010000000
VERSION     DAVBDG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.085"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(112..375)
                     /locus_tag="VQ526_001865"
                     /pseudo
     CDS             complement(112..375)
                     /locus_tag="VQ526_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            573..2339
                     /gene="patB"
                     /locus_tag="VQ526_001866"
     CDS             573..2339
                     /gene="patB"
                     /locus_tag="VQ526_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2555546.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2478..3167
                     /locus_tag="VQ526_001867"
     CDS             2478..3167
                     /locus_tag="VQ526_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2555547.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3171..3350
                     /locus_tag="VQ526_001868"
     CDS             3171..3350
                     /locus_tag="VQ526_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555548.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3337..4686
                     /locus_tag="VQ526_001869"
     CDS             3337..4686
                     /locus_tag="VQ526_001869"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2555549.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4810..6270
                     /locus_tag="VQ526_001870"
     CDS             4810..6270
                     /locus_tag="VQ526_001870"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2555550.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYESLGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6284..6718
                     /locus_tag="VQ526_001871"
     CDS             6284..6718
                     /locus_tag="VQ526_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2555551.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2114 a         1300 c         1460 g         1891 t
ORIGIN      
        1 aaggtggatg atggctacat tgtaatgttt tccttattct aacttagtct taagataggc
       61 cttaaaagca ggtgaaaagc gagggcatgc tttggcagct tgtatgagtg cctaccgcag
      121 atgaggggaa ccccgtttga tcattcttcc agctaaatca atctgatctg actgataaat
      181 agaagaatcc agtccagcga aagcttgtaa ttgagcagga ttatcaaagg catgaatatt
      241 ttgaatctcg gctaaaatga ccgcccctaa acgattccca atcccagtaa ccgtcgtgat
      301 gatcgagttg aactcagcca tcaagtcatt gacacatgtt tccgccttgt caatgagcct
      361 cttgtaatgt ttgatgtttt cattacacga gataaaacgt ttatgcgtta tcaaactcat
      421 gaccaattga aacaaaaagc tgtggttaga tcctttcgga aattgtcaag cgattggagg
      481 aaatgaacta atccacagcg gcttattcca agtataccac ttgggctttg gcagtagcta
      541 actgcgctaa atataatata aggaggagta aaatgaagac agttcaattt ttttggcatt
      601 attttaaggt ttacaagttc tcatttgtag ttgtcatcct gatgattgtt ctggcgactt
      661 ttgcccaagc cctctttcca gtcttttctg gacaagcggt gacgcagcta gccaatttag
      721 ttcaagctta tcaaaatggc aatccagaac ttgtatggca aagcctatca ggaatcatgg
      781 tcaatcttgg cctgctggtt ttggttctat ttatctctag tgtaatatac atgtgtctca
      841 tgacgcgcgt gattgcagaa tcgaccaacg agatgcgcaa aggcctcttt ggtaagcttg
      901 ctcagttgac ggtttctttc tttgaccgtc gacaagatgg cgatatcctg tctcatttta
      961 ccagtgattt ggataatatc ctccaagcct ttaacgaaag cttgattcag gtcatgagca
     1021 atattgtttt atacattggt ctgattcttg tcatgttttc gagaaatgtg acgctggctc
     1081 tcatcaccat tgccagcacc ccattggctt tccttatgct gattttcatc gtgaaaatgg
     1141 cacgcaaata caccaacctc cagcagaaag aggtagggaa gctcaacgcc tatatggatg
     1201 agagcatctc aggccaaaaa gccgtgattg tgcaaggaat tcaagaggat atgatggcag
     1261 gatttcttga acaaaatgag cgcgtgcgca aggcaacctt taaaggaaga atgttctcag
     1321 gaattctttt ccctgtcatg aatgggatga gcctgattaa tacagccatc gtcatctttg
     1381 ctggttcggc tgtacttttg aatgataagt ctattgaaac aagtacagcc ctaggtttga
     1441 ttgttatgtt tgcacaattt tcacagcagt actaccagcc tattatccaa gttgcagcga
     1501 gttggggaag ccttcagttg gcctttactg gagctgaacg aattcaggaa atgtttgatg
     1561 cagaggagga aatccgacct gaaaaggctc caaccttcac taagttgcaa gaaagtgttg
     1621 aaatcagtca tatcgatttt tcatacttgc ctgataaacc tattttgaaa gatgtcagca
     1681 tttctgcccc taaaggccag atgacagcag ttgttgggcc gacaggttca ggaaaaacga
     1741 ctattatgaa cctcatcaat cgcttttatg atgttgatgc tggtggtatt tattttgatg
     1801 gtaaagacat tcgtggctat gacttagata gtcttagaag caaggtggga attgtattgc
     1861 aagattcggt cttgtttagc ggaacgattc gagacaatat ccgatttggt gtgccagatg
     1921 ctagtcagga aatggttgag gtagcagcaa aagcaaccca cattcacgac tatatcgaaa
     1981 gtttgcctga taagtacgat actcttattg atgatgacca gagcatcttt tcaacagggc
     2041 agaagcaatt gatttcaatc gctcgaaccc tgatgacaga tccagaagtt ctcattctcg
     2101 atgaagcaac ttcaaacgta gatacggtga cagaaagcaa gattcagcat gccatggagg
     2161 tggttgtagc aggtagaact agtttcgtca ttgcccaccg cttgaaaacc attctcaatg
     2221 cagatcagat tattgtcctt aaagatggag aagtcattga acgtggtaac caccatgaac
     2281 ttttgaagct aggtggcttt tattcagaac tctatcacaa tcaatttgtt ttcgaatgag
     2341 aaagaagttg tcctatgtgg gcagcttttt cttgtccata aaaaatgttt atcacagcct
     2401 taaaaaaaac atattagacg aaagtcattt tgagtgatat gataggacta tcgttagcat
     2461 tcgaaaggag aggcatcatg gctagaacgg ttgtaggagt tgctgcaaat ctatgtcccg
     2521 tagacgcaga aggcaaaatc attcattcat ctgtatcttg tagattcgca gagatcattc
     2581 gtcaagtcgg tggtctccct ttagtcattc ctgttggtga tgagtcagtt gtacgtgatt
     2641 atgtggaaat gattgacaaa ctcattttga caggaggcca aaatgttcat cctcagtttt
     2701 atggagagaa aaagaccgtc gagagcgatg attacaatct ggtccgtgac gaatttgaat
     2761 tggcactctt gaaggaagcg cttcgtcaga ataaaccaat tatggcaatc tgtcgcggtg
     2821 tccaacttgt caatgttgcc tttggtggaa ccctcaatca agaaatcgaa ggtcactggc
     2881 aaggactacc tttcgggaca tctcactcta ttgagacagt agaaggaagc gtggtggcta
     2941 agctatttgg gaaagaaagt caggtcaatt cagtccatcg tcaaagcatt aaagatttgg
     3001 cacccaattt ccgtgtaact gctattgatt caagagacca aactattgaa gcgattgagt
     3061 ctatcgacga gcaccgcatt atcggtttac agtggcatcc agagtttctg gttaatgaag
     3121 aagatggcaa tttagaatta tttgagtatt tattgaatga actgtaacga ttggaacatc
     3181 tggtcgttct ttcttttatt ttttcaaaat ttttagaatg tggtatttta cgcaaacgtt
     3241 tgaattctga taaaaattgg agaaattcga caaaaaaact tgaaaaaaac gaaggtaagc
     3301 gttatgatag aaaagaagaa atattggagg aagaacatgt cacatattaa atttgattat
     3361 tcaaaagttt tagacaaatt tgttgcacca catgaagtgg aatacatgca atcacaagta
     3421 acagcagcag atgaattgat ccgtaaagga actggtgctg gtagcgactt cttgggatgg
     3481 ttggaccttc ctgaaaaata cgaccgcgaa gaatttgacc gcatcttgaa agctgctgag
     3541 caaatcaagt cagacagcga tgttttggtt gtgatcggta ttggtggatc ttaccttggt
     3601 gcaaaagcag caatcgactt cttgaatcac cattttgcta acttgcaaac aaaagaagaa
     3661 cgcaaagctc cacaaatcct ttacgcagga aactcaatct catctactta ccttgctgac
     3721 ttggtagagt atgttgcaga caaagacttc tcagtaaacg taatttctaa atcaggtaca
     3781 acaactgaac cagcgattgc tttccgtgtc tttaaagaac tcttggttaa gaaatacggt
     3841 caagaagaag ctaacaaacg tatctatgca acaactgacc gccaaaaggg tgctgttaag
     3901 gttgaagcag acgctaacgg ttgggaaaca tttgttgttc cagatgatat cggtggacgc
     3961 ttctcagtat tgacagccgt tggtttgctt ccaatcgcag catcaggagc tgacatcaaa
     4021 gctcttatgg aaggtgcgaa tgcagctcgc aaagactaca cttcagacaa aatctctgaa
     4081 aacgaagctt accaatacgc agctgttcgt aacatccttt atcgtaaagg ctatgcaact
     4141 gagatcttgg taaactatga gccatcactt caatacttct cagaatggtg gaaacaattg
     4201 gctggtgaat cagaaggaaa agaccaaaaa ggtatctacc caacttcagc caacttctca
     4261 actgacttgc actcacttgg tcaatttatc caagaaggaa ctcgtatcat gtttgaaaca
     4321 gttgtccgtg ttgacaaacc tcgtaaaaac gtgcttattc ctactttgga agaagacctt
     4381 gacggacttg gttaccttca aggaaaagac gttgactttg taaacaaaaa agcaactgac
     4441 ggtgttcttc ttgcccacac agatggtgat gtaccaaaca tgtatgtgac tcttccagag
     4501 caagacgctt tcactcttgg ttacactatc tacttcttcg aattggcaat tgccctttca
     4561 ggttacttga atgctatcaa cccatttgac caaccaggtg ttgaagctta taaacgtaac
     4621 atgtttgccc ttcttggaaa accaggattt gaagaattga gcaaagaact taacgcacgt
     4681 ctataataga agaaaagagt ggtttaccca ctctttttac tctctttatc catagaaatt
     4741 ggactcagtc aagacttgtg atataatata gaaagcaaaa aggcagatgc ctagataata
     4801 ggagaaacta tgtcaaaaga tatccgcgta cgttacgcac caagtccaac aggactacta
     4861 cacatcggaa atgctcgtac agcattgttt aattacttgt atgcgcgcca tcatggtgga
     4921 acatttctca tccgtatcga agatactgac cgtaaacgcc atgtcgagga tggtgaacgt
     4981 tcacaacttg aaaaccttcg ctggttaggc atggattggg atgaaagtcc agaatcacat
     5041 gagaattatc gccagtctga gcgtttagac ttgtatcaaa aatacattga ccaactatta
     5101 gctgaaggaa aagcctataa atcttacgtt acagaagaag agttggcagc tgaacgcgaa
     5161 cgccaagaag tagctggcga aacaccacgc tacatcaatg aataccttgg tatgagtgaa
     5221 gaagaaaaag cagcttacat cgcagaacgt gaagcagcag ggatcatccc aactgttcgt
     5281 ttggctgtca atgagtcagg tatctacaag tggcatgata tggtcaaagg cgatatcgaa
     5341 tttgaaggtg gcaatatcgg tggtgactgg gttatccaaa agaaagacgg ttacccaact
     5401 tacaactttg ccgttgccat cgatgaccac gatatgcaaa tctctcatgt tatccgtgga
     5461 gatgaccata ttgctaatac accaaaacag cttatggtct atgaatctct tggttgggaa
     5521 gctccagagt tcggtcacat gaccttgatt atcaactctg agactggtaa gaagttatct
     5581 aaacgtgata ctaatacact tcaatttatc gaagattacc gaaagaaagg ttaccttcca
     5641 gaagcagtct ttaactttat tgctcttctt ggttggaacc caggtggtga agatgagatt
     5701 ttctctcgtg aagaactcat taaacttttt gatgaaaacc gcctcagcaa gtcaccagca
     5761 gcctttgatc agaaaaaact agactggatg agcaatgatt atatcaagaa tgcagacctt
     5821 gaaaccatct ttgaaatggc aaaaccattc ttagaggaag caggccgttt gactgacaag
     5881 gctgaaaaat tagttgagct ctataaacca caaatgaaat cagtagatga gattatccca
     5941 ttgacagatc ttttcttctc agatttccca gaattgacag aagcagagcg cgaagtcatg
     6001 acgggtgaaa cagttccaac agttcttgaa gcattcaaag caaaacttga agcgatgaca
     6061 gatgataaat ttgtgacaga aaatatcttc ccacaaatta aagcagttca aaaagaaaca
     6121 ggtattaaag ggaaaaatct tttcatgcct attcgtatcg cagtttcagg cgaaatgcat
     6181 gggccagaat taccagatac aattttcttg cttggacgtg aaaaatcaat tcagcatatc
     6241 gaaaacatgc taaaagaaat ctctaaataa agaggataca ataatggaca tctgggaaaa
     6301 gatgtacgaa gaagcacaga aactatacaa tccacatgaa gtatcagact ttgtttatgc
     6361 caatcatgtt gtagctgcag tagaagcaga agatggacaa atatttacag gattctgtat
     6421 ggagggaacc tgtggtgttt tccatctctg cgcagaacgg gcggccctct tcaatatgta
     6481 ccaattttca ggacaaacta aggttaagaa agtattagcc tttcgagaca aaccacctta
     6541 tggtggaagt tcagccatgc cttgcggtgc ttgtagagaa ttccttttag agcttaatgc
     6601 tgaaaataaa gatgcagaat tcatgatgga ctataatata agaaaaacag ttaaagtcgc
     6661 agaactaatc ccttattggt ggggagaaga acgtgcttct aagtttaatg agcgatagaa
     6721 gagtcagtat aattttggct ctttttacta taagttgaaa aaata
//
LOCUS       DAVBDG010000085         6734 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBDG010000085 DAVBDG010000000
VERSION     DAVBDG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.086"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(354..533)
                     /locus_tag="VQ526_001872"
     CDS             complement(354..533)
                     /locus_tag="VQ526_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555552.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(675..824)
                     /locus_tag="VQ526_001873"
     CDS             complement(675..824)
                     /locus_tag="VQ526_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555553.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            1106..1573
                     /locus_tag="VQ526_001874"
     CDS             1106..1573
                     /locus_tag="VQ526_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2555554.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            1606..1703
                     /gene="ffs"
                     /locus_tag="VQ526_001875"
     ncRNA           1606..1703
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQ526_001875"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1760..2203
                     /locus_tag="VQ526_001876"
     CDS             1760..2203
                     /locus_tag="VQ526_001876"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2555555.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            2205..2720
                     /locus_tag="VQ526_001877"
     CDS             2205..2720
                     /locus_tag="VQ526_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2555556.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            2734..4095
                     /gene="radA"
                     /locus_tag="VQ526_001878"
     CDS             2734..4095
                     /gene="radA"
                     /locus_tag="VQ526_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2555557.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFFIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            4168..4665
                     /locus_tag="VQ526_001879"
     CDS             4168..4665
                     /locus_tag="VQ526_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2555558.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            4690..4884
                     /locus_tag="VQ526_001880"
     CDS             4690..4884
                     /locus_tag="VQ526_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555559.1"
                     /translation="MKKSSILLLCIGLQYETIYYTDGSRSGAEYGLMGISIFLALFYM
                     IPALYFLFRIGKNGNCQRRF"
     gene            4866..5489
                     /locus_tag="VQ526_001881"
     CDS             4866..5489
                     /locus_tag="VQ526_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2555560.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSTFLNA
                     FESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            5634..6602
                     /locus_tag="VQ526_001882"
     CDS             5634..6602
                     /locus_tag="VQ526_001882"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2555561.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
BASE COUNT         1863 a         1156 c         1616 g         2099 t
ORIGIN      
        1 tggtactacc tcaaaccaga cggaacactg gcagataagc cagacttcac agtagagcca
       61 gatggcttga ttacagttaa ataaatagaa aggaaacttt ctaaattgtt ctttcaccgc
      121 aggctcaggc ttgcggtttt tttgtttgtc tgaatcaaga aagcgtctaa ccaaccgaca
      181 ccaatgtcgg tagcaaaata aatggtttgc ctaaaaaatt agagttaagt tctatttttc
      241 aaaaacacgc attttgaacg attagaaaca gaaatctaaa tcctattgtt caaaaaaacg
      301 tttacatgaa aaatagggag gtgttttgtt aaaaataaaa acagtgatcg aaatcactgc
      361 ttatcagttg tagcaaattc ataaagtttt tctgctgtga gaagtgccat tttgtccatg
      421 cttgtttttc cttttctgag gtcagaaaca gtagtccatg gaactccagc accttgggaa
      481 atagcagatg tagacatcga actgtctaac aattcttgaa taacttctct catcttattt
      541 gtccttttta ttttttagat aaatatatac attgattgca attataaaaa tagctattgc
      601 actaaccatt gcatttcctc tttccatttg ataaaataga ggtgtaaggg gctttcgccc
      661 caacctctta gcgtttacct ttttctttga cgggatttcg gtttacgctt tttgttttgc
      721 cttgcgaccg ttattgcggt cactagactt gcgatagcag ttaccgtttc aggaatattg
      781 tctattgcct tttcaagtaa cctgagccaa tcttctttgt tcaacttcat cacccccttt
      841 ccttatcttg attatattat atcacggtac accgagaaag tcaagcgttt tgatgaagtt
      901 ttttttaatt ttttcaaaaa aaaatagacc ttgtccagag gtcggggagt tggagggggc
      961 accctccaaa agcattgatt taataaggtt ttattttacc tttttcataa taatctccct
     1021 atagagtcac cgcattcggt ggcttttttt gtgttgggat tcatgatata ataataaaat
     1081 cgataagtag gaaaagagaa aagagatgta ttatacgctt gaagaaaaag aagtctttat
     1141 gagggaggct ttgagagagg ctgagattgc tcttgaacac gatgaaattc caattggttg
     1201 tgtgattgtc aaagatgggg aaatcattgg tcgtgggcat aatgcgcgtg aggaattaca
     1261 gcgagcggtt atgcatgcgg aaattatggc tatagaggat gcgaacttga gtgaggagag
     1321 ctggcgcttg ctggattgca cactttttgt gaccattgaa ccttgtgtca tgtgtagtgg
     1381 agcgattggg cttgcccgca ttccaaatgt ggtctatggg gctaaaaacc agaaatttgg
     1441 cgctgctggg agtttgtaca atatcttgac agatgagcgt ctcaatcatc gtgtggaggt
     1501 tgaaatagga attttggaag atgaatgcgc agctatcatg caggattttt ttagaaatag
     1561 acggaaaaaa taattttgct tttaaaatga ataggaatgt gatataataa atagtggagc
     1621 aacagttctg cgtgaagcgg gtcaggggag gaatccagca gccctaagcg atttgaattg
     1681 tgtgctcttt ttttcgtgct ttttccgaat aaataagata gaataatcta gaataaatga
     1741 taatagaaaa gagaaaatta tgaaaattcg tggttttgaa ttggtttcga gttttacaga
     1801 tgaaaattta ttgcccaagc gtgagacagc gcatgcggct ggttacgact taaaggttgc
     1861 tgtgcgtaca gttgttgcgc caggagagat tgtcttggtt ccgacagggg ttaaggctta
     1921 tatgcagccg actgaggttc tctacctcta tgatcgttct tcaaatcctc gtaagaaggg
     1981 cttggtttta attaactcag ttggggtcat tgatggggat tattatggaa atcctggaaa
     2041 tgaagggcat atttttgcgc agatgaagaa tatcacagat caagaggttg ttcttgaagt
     2101 tggggaacgt attgtccagg ctgtttttgc tactttctta attgcagatg gagatgcagc
     2161 tgatggcgtt cgaactggtg gatttggatc gacagggcac tagaatgaag attatctttg
     2221 tacgtcatgg ggagccagat taccgtgagt tagaggagcg ttcttatata ggatttggga
     2281 tagatttggc acccttgtct gagatgggac ggcagcaagt ccagaaattg agcaaaaatc
     2341 ctttactctc gtcagctgaa ataatcgtat cttctgcagt cacaagagct ttagaaacgg
     2401 cttcgtatgt ggtctgtgct acgggtcttc ctttaagagt agagccttta ttacatgaat
     2461 ggcaggtcta taaaacagga atagaaaact ttgaaacagc tagaagactg tttttagaaa
     2521 acaaggggga gttgcttcct aatagtccta ttcaatatga gacagctacg gaaatgaagt
     2581 ctcggtttct agaatgtatg tctaagtatc gagaacatca gactgtggta gttgttgctc
     2641 atcgaatgct catgcgccag tttgtgccaa atgagaagat tgatttttgc caagtgattg
     2701 agtgtgagtt agagatatag aaagaggttt gtcatcgcaa agaaaaaagc gacatttgta
     2761 tgtcaaaatt gtgggtataa ttcccctaaa tatctgggac gttgccccaa ctgtgggtct
     2821 tggtcttctt ttgtggaaga ggttgaggtt gccgaggtca agaatgcgcg tgtgtccttg
     2881 acaggtgaga aaaccaagcc catgaaacta gctgaggtga cttccatcaa tgtcaatcga
     2941 accaagacgg agatggagga attcaaccgt gtgcttggag gcggagtggt accaggaagt
     3001 ctcgtcctca tcggtgggga tcctgggatt gggaaatcaa ctcttctcct acaagtctca
     3061 acccagttgt cccaagtggg gacagttctc tatgtcagtg gggaggagtc tgcccagcag
     3121 attaaattac gtgcagagcg cttaggtgat attgatagtg agttttatct ctatgcagag
     3181 accaatatgc agagtgttcg tgcagaagtg gagcgtatcc agccagactt cttcattatt
     3241 gactctatcc agaccattat gtctcctgag atttcagggg tgcaggggtc tgtttctcag
     3301 gtgcgtgaag tgaccgctga actcatgcag ttggccaaga ccaataacat tgccatcttt
     3361 atcgtaggtc atgtgaccaa agaaggaacc ttggctgggc ctcgtatgtt ggagcatatg
     3421 gtggatacgg tgctttactt tgaaggggag cgtcaccaca cctttcgtat tttgagagcg
     3481 gtcaaaaatc gttttggttc cactaatgag attgggattt ttgagatgca gtcgggcggc
     3541 ttggttgagg tactcaatcc gagtcaagtt ttcctagaag agcgtttgga tggggcgact
     3601 ggttcctcca tcgttgtaac catggaaggg acgcgtccga ttttggcgga ggttcaggct
     3661 ttagtaacac cgaccatgtt tggaaatgcc aagcgtacta cgacaggact tgattttaac
     3721 cgtgctagct tgattatggc tgttttggaa aaacgggcag ggcttctctt gcaaaatcag
     3781 gatgcctatc tcaaatctgc tggtggtgtt aaattggatg aacctgccat tgacttggct
     3841 gttgcagttg ctattgcttc gagctacaaa gacaaaccaa ctaatcctca ggaatgtttt
     3901 gtcggagaac tgggcttgac aggagagatt cggcgcgtga atcgtattga gcaacgcatc
     3961 aacgaagctg ctaaactggg ctttactaag atttatgtac ctaagaattc cttgacagga
     4021 atcactctgc ctaaggaaat tcaggtcatt ggcgtgacaa cgattcagga agttttgaaa
     4081 aaggtctttg cataatccgt gacaaattct cttaaaaatg ataagatagg agaaatattt
     4141 gactatcaaa ttttcaagga gggaatcgtg tcgtattttg aacagtttat gcaagccaat
     4201 caggcttatg ttgccctaca tgggcagtta aatctgccac ttaaacccaa aacaagagta
     4261 gctattgtga cctgtatgga ctctcgtctg cacgttgcgc aagctctggg cttggcactt
     4321 ggggatgctc atatcttgcg gaatgcaggt ggtcgagtga ctgaagacat gattcgttcg
     4381 ctagttattt cccagcaaca aatggggaca agagagattg tggtattgca ccatacagac
     4441 tgtggtgctc agacctttga aaatgaacct tttcaggagt atttaaaaga ggaattaggt
     4501 gtagatgtgt cagaccagga tttcttgccc ttccaagata tagaagagag tgtacgcgag
     4561 gatatgcaac tgcttatcga gtctccccta ataccagacg atgtcattat ctctggtgcc
     4621 atttacaatg ttgatacagg aagtatgaca gtcgtagaat tataaatact tcatttagaa
     4681 agaaagtgta tgaagaaaag cagtatttta ttgctatgta ttggtttaca gtatgaaacc
     4741 atctactata cggacggttc gaggtcaggt gcggaatatg gactaatggg aatttctatc
     4801 tttctagctc tcttttacat gattccggct ctttattttc tcttccgtat tgggaaaaat
     4861 gggaattgcc aaagaaggtt ttgattctgt ctttattggg agggatgttc ctttcaggct
     4921 ggttgtctag ttttgctaat acttatatcc atgatttact gggggttctt ttcccagata
     4981 gtacattttt aaatgccttt gaaagtgcta ttgtggctcc tttggtagaa gaacccttga
     5041 aattattgcc acttgttttt gttttggctt tgattcctgt gcgaaaatta aaatctttgt
     5101 ttttacttgg aattgcttcc ggtttgggat tccaaatgat tgaggatatt ggttatattc
     5161 gtacggattt gccagagggc tttgacttta ctatttcgcg aattttagag cgtatcatct
     5221 caggaattgc ctctcactgg actttttcag gtctagctgt agtaggtgtt tacttgcttt
     5281 acagagccta taaaggacag aaggttggca agaaacaggg ccttattttt ctaggtttag
     5341 ccttgggaac tcacttcttg tttaactctc cttttgtgga gttggaaaca gagttgcctt
     5401 tagcgattcc agtggttacg gctattgctc tctatggttt ttatcatgct tattgctttg
     5461 ttgagaaaca caatgagttg atgacctaga atatttttca aaagaatgat gcaagggtac
     5521 aaatatggtg cccttctttt atttttgatt gaaaaatagt gcaaaaagcg ctacaatggt
     5581 agatggaaaa tcttgtgaaa ggcacaagtt atacatatat accggaggaa atcatgtctt
     5641 tttctgattt aaagctgttt gccctttctt ctaataaaga attggcagaa cgtgtggcgc
     5701 aggagattgg gatagagttg gggaaatcaa gtgttcgcca attttcagat ggagagattc
     5761 aggtcaacat tgaagaatca atccgtggga aacacgtctt tatcttacaa tcaactagtt
     5821 cgcctgtaaa tgacaatctg cttgaaattt tgattatggt agatgctttg aagcgtgcga
     5881 gtgcagaatc tgtcaatgtt gtcatgcctt actatgggta tgcacgtcag gatagaaagg
     5941 cgagagcgcg tgagccaatc acttcaaaac ttgtcgcaaa tatgcttgaa gtagctggag
     6001 tggatcgttt attgaccatc gacttgcatg ctgcgcaaat tcaaggattc tttgatattc
     6061 ctgtggatca tttgatgggt gctcctctga ttgcagatta ttttgagcgt cgtggtatgg
     6121 ttggttctga ctatgtggtt gtcagcccgg accatggagg ggtgactcgt gctcgtaagt
     6181 tggcagaatt tttgaaaaca tctatcgcta ttattgataa acgtcgtagc gttgataaga
     6241 tgaatactag tgaagttatg aatatcatcg gtaaggttga aggcaagact tgtatcttga
     6301 ttgatgatat gattgatacc gctggaacga tttgtcatgc ggcagatgct cttgcggaag
     6361 ctggtgctgt tgaagtctat gcaagctgta cgcacccagt tctttctggt cctgctacgg
     6421 acaatatcca aaaatcagct attaagaaat tggttgtttt ggataccatc tatctgccag
     6481 aagagcgttt gattgataag attgagcaga tttcaatcgc tcatctccta ggggatgcta
     6541 tcgtacgtat tcatgaaaaa cgaccacttt ctccactttt cgatattgag aaaaaaattt
     6601 aatgaccaag cctgagatga ttctcaggtt ttttcgtctt ttttccgaat aaatagatag
     6661 tagcggtgaa tctagtaaag ctagatttaa aactgtgata taataaaaag agaagaaata
     6721 tgactgtacg tcat
//
LOCUS       DAVBDG010000086         6532 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBDG010000086 DAVBDG010000000
VERSION     DAVBDG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.087"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(58..384)
                     /locus_tag="VQ526_001883"
     CDS             complement(58..384)
                     /locus_tag="VQ526_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2555562.1"
                     /translation="MVEYISRKYFLTEEEFQAEKICNQSLVELFQKKYSWHSLNDFGS
                     STYDKNYASIFYEYWRSFLTVDKLVENLGSVQAVLDSYHLWANTEKTFPLLDWFVQQK
                     LIEKEI"
     gene            complement(351..635)
                     /locus_tag="VQ526_001884"
     CDS             complement(351..635)
                     /locus_tag="VQ526_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2555563.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMTLISGSKRGWLNILVASIS"
     gene            complement(908..1750)
                     /locus_tag="VQ526_001885"
     CDS             complement(908..1750)
                     /locus_tag="VQ526_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2555564.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(2006..3397)
                     /locus_tag="VQ526_001886"
     CDS             complement(2006..3397)
                     /locus_tag="VQ526_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2555565.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKMEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEAAILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(3632..4009)
                     /locus_tag="VQ526_001887"
                     /pseudo
     CDS             complement(3632..4009)
                     /locus_tag="VQ526_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            4242..5219
                     /locus_tag="VQ526_001888"
     CDS             4242..5219
                     /locus_tag="VQ526_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2555566.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFRKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            5216..6298
                     /locus_tag="VQ526_001889"
     CDS             5216..6298
                     /locus_tag="VQ526_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2555567.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     SEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(6362..>6532)
                     /locus_tag="VQ526_001890"
     CDS             complement(6362..>6532)
                     /locus_tag="VQ526_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2555568.1"
                     /translation="IEQVLKEIRKRGFKNKAFQTLEDVMNQLQDIIQGLEKEVIKPIV
                     NRRWTRMLFESR"
BASE COUNT         1951 a         1454 c         1091 g         2036 t
ORIGIN      
        1 tctgccactg atgcagtgaa atgtcaattt cttagacatt gtttactcct ttagttttta
       61 tatttctttt tcaattaatt tctgctgaac aaaccaatct aacaagggaa aagttttttc
      121 tgtatttgcc cataaatgat aagaatctaa gaccgcttgt acactaccta aattttctac
      181 caacttatct actgtcaaaa agctgcgcca gtattcataa aaaatacttg catagttctt
      241 atcataagtc gaagaaccaa aatcattcaa tgaatgccaa ctatacttct tctgaaaaag
      301 ttctacgaga gattgattac aaattttttc cgcttgaaat tcctcttctg tcaagaaata
      361 cttgcgacta atatattcaa ccatcccctc ttcgaaccag atataagagt cataaccatt
      421 cgtctattat ttgtataggc tggaacagcg ctttccctaa taatctgtgt agcagcatta
      481 aaatctgacc aaataacggc ttgcggtaaa tcacagactg caagttcatt ctttaaaaaa
      541 gattgataat ctttcaactt ttcagtattt cttactacaa aatcacgaaa tgtagctagt
      601 tgactatcgt cttttacaag ataaaggttg tccacggctc tcctttcttt cacacaatag
      661 actgaataag caaaagaacc acatctgaca atcctaggtt gatttaacct aggggcgtca
      721 gaaacgcggt tccaccctaa tttattactt cattgttttt gaaaatacta cagaaagcgc
      781 cagctcatta ttttgcccta cttggctccc actgtcccaa gctcgcttga agaacgccat
      841 aagactttct ttcctgctga ctattatatc aaaaattaga ataatgtaca attcttttta
      901 tgattttcta gaaatccaca tcatcaactc gtggagcacc cgattgaaca gcaatcgttt
      961 ttaaggtttc tctctcctca tgactcagtt caattccaaa gcaatcaaga ttagcttgaa
     1021 tacgagaggt tgtgacagat tttggaagtg gtaaaaatcc ttctgccaag ctccaggcca
     1081 aggctatctg agcaacagac tttccgtgat ttgctgctat ttcttggact tgcttgctat
     1141 caaacagttc tccttgtcca aaaggccccc aagcttccaa taaaattccc ttttcacgac
     1201 agtaagctac gacttcctct tgatacacac ctggcgccaa gcgaacttga ttgaccgcag
     1261 gaacgatagt tgcagtttca agcaaggcat ccaaatgatg gggaagaaaa ttgctaacgc
     1321 cgatagcacg gattttccct tcttgataga ggtcttccat cgctctccaa acttccgcat
     1381 tgcgagtttt ccatgcgtca ttttctctga gcggttttgg gttcggccaa tgaatcaaat
     1441 acaaatccaa ataatccaag cccagttttt ctatagattt ttccaaagct tgacgagttt
     1501 gctcataggt ttgctgacta ttccaaagct tggtagttac gaacatttct tcacgtggaa
     1561 ctccgctatc tttgattgct tgaccaacac tttcttcatt ctgataaatc gccgccgtat
     1621 caatatgacg ataaccagcc ttcaaggctt ctaacactgc acgataggct tcttctccat
     1681 ccttagcctt aaaagttcca aatcccaata ctggaatttc tactccatta tttaattgat
     1741 aggtattcat atgcttctcc ttctatttta tactctcttc aaaccacatc aacgtcgcct
     1801 tgccgtaggt attgttactg acttcgtcag ttctatccac aacctcaaaa cactgttttg
     1861 agcaacctgc agctagtttc ctagtttgct ctttgatttt cattgagtat tattcatcaa
     1921 ttatagcata aaacattttt taagtagtac tatcttttgt aaaaaataag tctatcacat
     1981 atctgacaga cctatttttt cttgcttatt catcacgact ataatacagc tcatgagctt
     2041 ttaggcgagt attgagcatc tctggtatgg tcacaaaggt ataaccttga tttttcaaat
     2101 actcaatgac ccttggcaag gcattgactg tcggactgtg aatatcatgc atcaaaacga
     2161 tagagccgtt agctacttga tgctgaattt ccgtcaaaat agctgcttca tttttactct
     2221 tccagtccag actatccaca tcccacataa taaagctcaa atcgaggcta ttgcgaatat
     2281 catctgtaat agctccataa ggcggacgca tgagtttgga actagaaccc agcactttag
     2341 ttagcacatc ctcagtatca gtaatctgct ttttagcttc atcaagagat agttgcgaga
     2401 gaataggatg gctccaacta tggtttccaa caacatgacc ttcagatttt atcctcttca
     2461 ccaagtcctc attcccagaa acatttttcc caagcacaaa gaaagtcgct ttaatatcat
     2521 atttagctag ggtctctaat acctgcgggg tcgttgctgg atttggacca tcatcaaagg
     2581 ttagagcgac aactttttga tgtttcttat caaagtaaga ttggtacaag gccgcatctt
     2641 tttcgagtaa gtacgaagat tggataacat caaagaaagc agatactggc aaggctatct
     2701 cttctaaatt ttcaaccaca ggacttggat aaaggataat ctgactatcc ttgtaatcaa
     2761 aattccatgc agacaagtct tggtcagaga agttttttac aatctgctca ctctggtctt
     2821 gctccatttt tttatcctct atgaaggagg tcaactcttt tatcagctgt tccttagcct
     2881 tactagcatc tgaaaacagt tggtcaagtg taaaaggttg cccatcttct gttaagtgaa
     2941 cctttcctag actggtcttt tcagtctctt caatcttaaa agcaactaaa tcatagattt
     3001 gtttggtcac attacgatta acgactccct ttaaacctga ctcttcttgc tctgtatagt
     3061 agaaaacaag attgtcctta ctttctagct tgtccttaac atcttgattt atcagctccc
     3121 taacagagga aatcactttc tccccttgga gaggataata ggcaatcact tcggcttgcc
     3181 cctgacgaaa atgctccttc tgatttccct cactcaattg atcatctttc tcttttttga
     3241 gcgattcaat cttttgctca aaactttttt gctggtagat cttaaaagca ataaatccgc
     3301 ctaataggca aatagaaatt gctaaaatac caatcaaagc caataataca tgtctcgttt
     3361 tcccgtgtct gccacgtcct agtctacttt tattcatatt tatatcataa caaatttgag
     3421 caataatttc aatgatacaa tttggcaatc cacaattcct ctagcaagcc aaagcatctt
     3481 acatcaactc caacatccgt atctgtagag attatgtagt tttctatcta tggaatttct
     3541 agtagattga agctagaata gtagacctat acttctaaaa cattgttaga aatcgatttg
     3601 actgtcctga tcgatttgtc ctgttctcat ttcattttac tataaaacta tgaaaagcta
     3661 ggtcatctga gaagtaatta ctatcgacaa ttgcgatagc gtatactagt gcgatttgtt
     3721 tgtagctctg gtgggtatca ccttcacgtt ggcgaatttt ttctaggttt tgattaacct
     3781 aactgcacag ataagcccat aggcgattga tgaaaaaatt ctgatgatga acctgaattt
     3841 cgatgataac ttggattcca ttatctaact ccgccaagac gtctatacta gtatagaaat
     3901 cctgcgctga gtagggcatg gaaggcaaga cgtgaatgtt acttccctcc aaaatcgtca
     3961 cattttttgc tggcaagtcc agcatatcgc ggataaattg acaaatcatt agttgggctg
     4021 atacccggat gtctgagaat cctccttttc ctccttctat tatagcacat ttttaaatct
     4081 aggtttgcta gattctatgc ttctatctat ttattcggaa aagaaagctt ttcttcagaa
     4141 gaaagctaat agcttatttg aaattttctc ctctctgttg gatagtcccg tctatctatg
     4201 ttataatgaa agaaggattt aaaatcggaa aaggagtatt tatgctaaaa ttaggtgtca
     4261 tcggaactgg cgctatcagc catcatttca tagaagcagc ccataccagt ggagaatacc
     4321 aactggtcgc aatctattct agaaaactag aaactgcagc aacctttgct tctcgctatc
     4381 agaatatcca actattcgat caattagaag tcttcttcaa atcttctttt gatttagtct
     4441 atatcgccag tccaaactcc ttacattttg ctcaggcaaa agctgccttg tctgctggta
     4501 aacatgttat tctcgaaaag ccagctgtca ctcaaccaca agaatggttt gatttgattc
     4561 aaacagctga aaaaaataac tgttttatct ttgaagcagc tcgtaattac cacgagaaag
     4621 cctttactac tatcaaaaac tttttagcag ataagcaagt gttaggagca gatttcaact
     4681 atgccaaata ctcttccaag atgcctgact tgttgactgg acaaacacca aatgtctttt
     4741 cagaccgttt tgctggtgga gccctcatgg atttggggat ttatcccctc tatgctgctg
     4801 ttcgtctttt tagaaaagcc aatgacgcga cttaccatgc tcaacagctt gacaatagca
     4861 ttgacctaaa tggagatggt atcctcttct acccagacta tcaagttcac atcaaggctg
     4921 gaaaaaacat cacttccaat cttccttgcg agatttatac aacagatgga accttgactc
     4981 tcaacacgat tgagcatatt cgctcagcta tttttaccga ccaccaagga aatcaagtcc
     5041 agcttcctat ccaacaggct cctcatacga tgactgagga agtcgctgca tttgcacaca
     5101 tgatccagca accagatctg aatctctacc aaacttggct gtatgatgca ggatctgttc
     5161 atgagctact atataccatg cgccagactg ctggcattag atttgaggca gaaaaatgaa
     5221 aaccaaacta ccgactgaat ggcaagaact gagtgaccaa ctcggtttcc aagaattcac
     5281 ccccattcaa actcaactat ttgagcccct tcttgctgga gaaaacctcc taggagtgag
     5341 ccaaaccgga actggtaaga ccctagcgta tctcctacca agtcttctca gactacaaaa
     5401 gaaaaaagcc caacaactct tgattctagc accaaatacg gaacttgctg gacagatttt
     5461 tgatgtatgt aaaacgtggg cagaagctat cggattgact gctcaactct tcctttcagg
     5521 ttcaagtcag aaacgacaaa ttgaacgcct taaaaagggg ccagaaattc tgattggaac
     5581 tcctggccgt atctttgaac tcattaaact taaaaaaatc aagatgatga atgtggaaac
     5641 catcatcctg gatgaatttg accaattact agatgattct cagattcact ttgtagaaaa
     5701 aatcactcac tacgcacctc gtgaccacca actggtctac atgagtgcca cgaccaagtt
     5761 tgaccaagaa aagattgtgc cgaatacacg cactattgat ctctctgacc aaaaattgga
     5821 caatatccag catttctaca tgcaggtaga ccaacgtcat cgagtggata tgctacgaaa
     5881 actggctcat gtagaggatt tccgtggtct agtcttcttc aatagcctgt cagaccttgg
     5941 gaatgccgag gaaaaactac agtatcgtga tatattgact gtttccctcg ctagtgatgt
     6001 taatgttaaa tttagaaaaa tcatcttaga aaagtttaaa gataaccaac taaccctgct
     6061 tcttgcaact gaccttttgg ctcgtggaat tgatatcgat agcctagaat gtgtcgtaaa
     6121 ctttgatatt cctagagata gcgaaaccta cacacaccgt gctggccgta ctggtcgtat
     6181 gggcaaggaa ggatatgtta tcactctcgt cactcattca gaagaaatca aaaaacttaa
     6241 aaagtttgca agtatccgag aaattgtcct aaaaaaccaa gaactctata tcaaataaag
     6301 ccccattgct ctatacacat ccagtgtatg gagctttttt ataagcaatt caattttata
     6361 ctcatctgct ttcaaaaagc attctagtcc atctccgatt aacgatgggc tttatcacct
     6421 ccttctccag tccttgtatg atatcttgaa gttgattcat gacatcttcc aaagtttgaa
     6481 aggctttatt cttaaatcca cgtttacgaa tctctttcaa cacttgttca at
//
LOCUS       DAVBDG010000087         6488 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBDG010000087 DAVBDG010000000
VERSION     DAVBDG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.088"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..483
                     /locus_tag="VQ526_001891"
     CDS             <1..483
                     /locus_tag="VQ526_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET2555569.1"
                     /translation="KMVEYLVTELSHFEYNLLIEGTLRTIDVPKKTAQLLKNKGYKVQ
                     LALIAAKPKLSYLSTLIRYEELYAINPNQARATPKEHHDFIVNHLVENTRQLEELAIF
                     ERIQIYQRDRSCVYDSGTDEGSAADVLQECLFGKLSKVEEEMMKMGREQLRALKVKGE
                     "
     gene            487..1818
                     /locus_tag="VQ526_001892"
     CDS             487..1818
                     /locus_tag="VQ526_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002911647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555570.1"
                     /translation="MAGKKNNATSSWSGYNHQGKVGIFLALKELSELLQKDEDFCNYS
                     VQFEKEDGEDIDIVQGDIVISRHQVKAKTKGKYPNDYVDVLIGVKKKVENNIEIIGGF
                     NTDGVNENSRYLHTVCYVEGFGIPKKNFEFLFQNQRGAKPRFVENPYRIKLYQYPDGK
                     KYCGLSDVNESKIDGFCEAEIKKLLAKLSPNLKNDDEHIKETLFELKDLLCTKIRKAH
                     ETGRGSYPVINFSEIYTIIISIQKREKRAIHTARNMLERYWNLNSNKQNINKQNIDAS
                     LITDILNLPDKGFQQFLIDLHPQKDIKGALDNQTIFELLDQDIFEEIFCEFYKNINQD
                     FFDISELRYNSTKSSFRLSLINKNCKNGEVGELVQRIRNNEQFLKASFDVDYLVNGRI
                     NSPLFEQRQLDNGLEPPYSQRPSDRNRLFSNHLEFIDIDKTVKKLIGEQYE"
     gene            1811..2533
                     /locus_tag="VQ526_001893"
     CDS             1811..2533
                     /locus_tag="VQ526_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555571.1"
                     /translation="MNDFLASIMDSNGYNLIEGISLELFGNEHEFFLIEHFEIGEIQD
                     FFKTEKLENLISQFQKVDNKIQKNTSLFILIKVDNIHEFYNLYLNKIASIEEDEYYFR
                     KYVIFYTEEGLVELKPDADSLLQYIQENDDDSDEESLFDKFEKNMFFDDSYFIAMQLI
                     IKLPFISLPHSNDHFETIEDRIESRIKTAELLQQEKQVTQILELFNSDKIGSQLEDAT
                     IIYSLQQILEDGACENQEDFTL"
     gene            2505..5165
                     /locus_tag="VQ526_001894"
     CDS             2505..5165
                     /locus_tag="VQ526_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002911649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2555572.1"
                     /translation="MKIKKILLYNFKNFRHETVIDFSDNMTFLVGPNGFGKTTIFDAI
                     ELGLTGDISRINQNNPVTGENIKYNKPFFQNDISEPVIIKLWLEKSNVNQLVLVRKLI
                     NDRPDGGILYAPKRSIGQFKLFRQEEATDENFSSLDNDIKLSEIDQTTIDNFLEIDGE
                     YKLAKIFNLFNYIQQEETTFFLKKSEKDRSNDLSFLVKTDEIEDRISKIEKVNKAIND
                     NLKSLTEQQEKLTQQELDNIPYQRLFNQQVFQFDKESPFSVDNLSQSDDFKVTIQKII
                     EFKQSFSISEYQRKLERDNRKKEINTIDSALRFAILFSLIQRPNYQWQWEKYTIENPR
                     FFEYVLLENYLQTFETITHEYRRKQQLNQYWEDLSTDINQMTAQTFQYVQNDRLSNDF
                     ESLKVQLTSYQTLRESVGQADKNLSDLRQLRRKLGEKFDELRQHNHVDEDRCPFCNNQ
                     FDSFVDLKSSYDNYTDYLTEISSRDSQRLQEVQLSLNSRIQQVKQKITDEINSLTTNI
                     DKKLLDRLQELSGSYQSYSKYLEDFKTFIQSYTNMAPYQLENLEFDNYNHQYKSSLQE
                     FRSKLVVDDDVYRVLDINSLENIKGQLGDLREEFPELQFETYQLESSSYSKINMAMIN
                     SRLHELKQAIHLAIDEKYAINENLIADAENIFPTYFQSKVEVLEDIRIGDLEEKKLYI
                     DKQYKLVQNQQFQDLSRRIKILEKTAERLKEIHTIYKEEVKKFKIGIVKQLRIPFFIY
                     SAKMLQNYQQGMGIFLTYKKTTSSDNETVAIIRFKSDTKNDHDVMNQLSTGQLAVVSL
                     AFTLSLNTMFKLSDNLNFLMIDDPIQDMDAMNVLSFIEILRHGIIDRYQIILSTYSDH
                     NALFMGYKFANSYPKVDIDYKNVRELKGSL"
     gene            5532..5891
                     /locus_tag="VQ526_001895"
     CDS             5532..5891
                     /locus_tag="VQ526_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555573.1"
                     /translation="MGQEIKSIRKQFRITRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELISE
                     VNQVQPLSREFREKYMG"
     gene            5901..6266
                     /locus_tag="VQ526_001896"
     CDS             5901..6266
                     /locus_tag="VQ526_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HET2555574.1"
                     /translation="MVYRYRTNLKKVFLTGPELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQEQLQELSKGISELITG
                     VEKDFQVEVNRLREFYGSH"
     gene            6253..>6488
                     /locus_tag="VQ526_001897"
     CDS             6253..>6488
                     /locus_tag="VQ526_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HET2555575.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPEKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFTNNDKLYESRNDRQEKYQQT"
BASE COUNT         2403 a          869 c         1218 g         1998 t
ORIGIN      
        1 aaaatggtag agtatctagt aacagaattg agtcattttg aatacaatct tttgatagag
       61 ggaactttac gaacgattga tgttccaaag aaaacagcac aactcttgaa aaataaggga
      121 tataaagtac aattagcctt gattgcggca aagcctaagc tgtcctattt gagcaccctt
      181 atccgatacg aagaactgta cgctatcaat ccaaatcaag cacgcgcaac tccaaaagaa
      241 catcatgatt tcattgtaaa tcatctagtt gaaaatacac gacagttgga agaactagct
      301 atctttgaaa gaattcaaat ttatcaacga gatagaagtt gtgtatatga ttcaggaaca
      361 gatgaaggtt cagctgctga tgttctccaa gaatgccttt ttggaaaatt gagtaaggtg
      421 gaagaggaga tgatgaagat gggaagggaa cagttgaggg cgttgaaagt taaaggagag
      481 taatgagtgg ctgggaaaaa gaataatgcg acatctagtt ggagtgggta taatcatcaa
      541 ggtaaggttg gtatttttct tgcgttgaaa gaactaagtg agttgcttca aaaagatgaa
      601 gatttctgta actactctgt tcaatttgaa aaagaagatg gtgaagatat agacatagta
      661 cagggtgaca tcgttatttc aagacatcaa gttaaagcaa agacaaaagg aaagtatccg
      721 aatgactatg tggatgtatt gataggagtt aaaaagaaag tggagaataa tattgaaatc
      781 ataggaggat ttaatacgga tggtgttaat gaaaattcca gatacttaca tacagtttgt
      841 tatgttgaag ggtttggaat tcccaaaaaa aattttgaat ttttatttca aaatcagaga
      901 ggagcgaagc caagatttgt tgaaaatcct tatagaataa aattatatca gtatcccgat
      961 ggcaaaaaat attgtggatt gagtgatgtt aatgaatcta aaatagatgg attctgtgag
     1021 gcagaaataa aaaaattgct tgcgaaactt agtcccaatc tcaaaaatga tgatgagcat
     1081 attaaagaaa ctttatttga attgaaagat ttactctgta ctaaaatcag aaaggcacat
     1141 gaaactggaa gaggtagtta tcctgttata aatttttcag agatttatac tatcatcatt
     1201 tctattcaaa aaagagaaaa gagagcaatt catacagcta gaaatatgtt ggagagatat
     1261 tggaatctca acagtaataa gcaaaatatt aataagcaaa atattgatgc ttctttgatt
     1321 actgatattc taaatttacc cgataaagga tttcaacagt ttcttattga tttacatcct
     1381 caaaaagata taaagggggc attggataat caaaccattt ttgaattact tgatcaagac
     1441 atttttgaag aaatattttg tgaattttat aaaaatatta atcaggattt ttttgatatt
     1501 tctgagctac gttataattc aacgaagtca agctttcgac tgagtttaat taataagaat
     1561 tgcaaaaatg gagaggtggg ggaacttgtt caaagaatta gaaataatga gcagtttcta
     1621 aaggcgagct ttgacgtaga ttatcttgta aatggtcgaa taaactcacc tttatttgag
     1681 cagaggcaat tagataatgg tttggagcca ccatatagcc aacgaccttc tgataggaat
     1741 cgtctatttt caaatcatct tgaatttatt gatattgata aaacggtaaa aaaattaata
     1801 ggagaacaat atgaatgatt ttttagctag tattatggac tctaacggat acaatctgat
     1861 tgagggtatc tctttggagc tatttggtaa cgaacatgaa ttttttctta ttgaacattt
     1921 tgagatagga gaaattcaag atttttttaa aactgagaaa ttagagaatt tgatttctca
     1981 gtttcaaaaa gtagataata aaatccaaaa gaatacctcc ttatttattt taataaaagt
     2041 agataatatt catgaatttt acaaccttta cttaaataaa atagcatcca ttgaggagga
     2101 cgaatactat ttcaggaagt atgttatttt ttataccgaa gaaggtcttg tagaactaaa
     2161 gcctgatgct gattctttac ttcaatacat tcaagaaaac gacgatgatt cagatgaaga
     2221 aagtttattt gataaatttg agaaaaacat gttttttgat gatagctatt ttattgctat
     2281 gcaactgatt attaagttac cctttatttc tctccctcat tccaatgacc actttgaaac
     2341 aattgaagat agaatagagt caagaatcaa aaccgcggaa ttacttcagc aggaaaaaca
     2401 agtaactcag attttagagt tgtttaatag tgataagatt ggttcacaac ttgaagatgc
     2461 taccataata tatagtttac aacaaatttt ggaggatgga gcttgtgaaa atcaagaaga
     2521 ttttacttta taattttaag aattttagac atgaaacggt tattgatttt agtgataaca
     2581 tgactttctt agttggacca aacggatttg gaaagacgac tatttttgat gcaatagagc
     2641 taggtttgac aggtgatatt tctcgaatca atcaaaataa tccagtgact ggagaaaata
     2701 tcaagtataa taaacctttc ttccaaaatg atatttctga accagtgatt ataaaattat
     2761 ggttggaaaa atcaaatgtt aatcaattgg ttcttgttcg aaagttgata aatgataggc
     2821 cagacggtgg aattttatac gctccgaaga ggtctatcgg tcagtttaag ttgtttcggc
     2881 aggaagaagc aactgatgaa aactttagta gcttagataa tgatattaaa ttgtctgaaa
     2941 tagaccagac tacaattgat aattttttag agattgatgg ggaatataaa cttgctaaga
     3001 tttttaatct gtttaactat attcagcaag aagaaacgac atttttcttg aaaaaatctg
     3061 agaaagatag aagtaacgat ctctcatttt tggtaaaaac tgatgagata gaagatagaa
     3121 taagtaagat tgaaaaagta aataaagcta tcaacgataa tctgaaatcc cttactgagc
     3181 aacaagagaa acttactcag caagaactgg ataacatccc ctatcaaagg ttgtttaatc
     3241 aacaggtctt tcagtttgat aaagaaagtc cgttttctgt agacaacctc agtcagtcag
     3301 atgattttaa agttactatt caaaagatta ttgaatttaa acagagtttt tctatttctg
     3361 aatatcaacg aaagttggaa agagataacc gaaagaaaga aatcaatact attgatagcg
     3421 ctttacgctt tgctatttta ttttcactta ttcaaagacc taattatcaa tggcagtggg
     3481 aaaaatacac tatagagaat ccgagattct ttgaatacgt acttttagaa aattaccttc
     3541 aaacatttga gactatcact catgaatata ggcgaaaaca acaattaaat caatactggg
     3601 aggatctatc tactgatatt aatcaaatga cagcgcagac ttttcagtat gttcaaaatg
     3661 ataggttatc aaatgatttt gaatcactta aagttcaatt aacaagctac cagacgctaa
     3721 gagaaagtgt tggacaagca gataaaaatt taagtgattt acgacagtta cggagaaaat
     3781 taggtgaaaa atttgatgag ctaagacaac ataatcatgt tgatgaagat agatgtccat
     3841 tttgtaacaa tcagtttgat tctttcgttg acttgaagag ttcttatgat aattatacag
     3901 attacttaac tgaaatttca tctagagata gtcagcgatt acaagaagtt caattatcat
     3961 tgaattcaag aatccaacaa gtgaagcaaa aaatcactga tgaaataaac agtcttacta
     4021 ccaacataga taaaaaacta ttagatagat tacaagaatt gagcggttcg taccaaagtt
     4081 attctaaata tcttgaggat tttaaaactt ttattcagtc gtatactaat atggcacctt
     4141 atcaactgga gaatttagaa tttgataact ataatcatca gtataaatct agcttacaag
     4201 agtttcgatc taaattggta gttgatgatg atgtttatag ggttttagat attaatagtc
     4261 tggaaaatat aaagggacaa ctgggcgact taagagaaga atttcctgaa ttgcagtttg
     4321 aaacatacca attagaatca agttcatatt cgaagataaa tatggcgatg attaatagtc
     4381 gattgcatga attaaaacag gctatccact tagcaattga cgaaaagtat gccattaatg
     4441 agaatttgat agcagatgct gagaatatat ttcctacata ttttcaaagt aaagtggaag
     4501 tattagagga tattcgtatt ggagatctag aggagaagaa actctatata gataagcagt
     4561 ataaactagt tcaaaatcaa cagttccaag acttatcacg tcgaataaaa atattggaaa
     4621 aaactgcaga acggttaaaa gaaattcata ctatatataa agaagaggtg aaaaagttca
     4681 aaataggcat tgttaaacaa ctacgcatcc cattcttcat atactcagca aaaatgttgc
     4741 aaaattatca acaaggtatg gggattttct taacgtacaa gaaaacaacg agtagtgata
     4801 atgaaacggt agcaattatt agatttaaat ccgacactaa aaatgatcat gatgtaatga
     4861 atcagctaag tacggggcag ttagctgttg tgtcgcttgc atttacttta tctctgaata
     4921 ctatgtttaa gttatctgat aatttaaatt tcctgatgat agatgatcct attcaggata
     4981 tggatgcaat gaatgtattg tcctttattg agattttgcg tcacgggatt atagatagat
     5041 atcaaattat tttatcaacg tatagtgacc ataatgctct atttatggga tataagtttg
     5101 caaattcata tccaaaagtt gacattgact ataagaatgt ccgtgaatta aaaggttctt
     5161 tataaactgt ggtgagttaa ttaaaagagt aataattttc ttagagagca tcagctctct
     5221 tttttcttac cttatttttg atgatgtgat accttttcca aatcttccct gttatcctaa
     5281 tatcactcta agaaaatcaa tcctcaattt ttaactgggg tttgggggcg gagccaccaa
     5341 gttatcttat cgttggctgt caaaactaga aggttttgat aggctggcga tatgattttt
     5401 gggatattgt ggacacaata tctgagctcg caaagaccga acaagaagaa agtgaggtct
     5461 ttaggaagcg tactgacaat gtgaggtaga cttacactgt cagacaagta gaatgttgaa
     5521 gggatatcag aatgggacaa gaaattaagt caatccgaaa gcaatttaga attacgagac
     5581 aagaagaaaa acagataaaa gaaatgatga gggaacaaaa agtggatagt ttctcagaat
     5641 ttcttcgtca aaatttattg aaaaagaatt atcaggatag aatttttgaa agttggttct
     5701 ccctttggca gtctcaaaag tttgaacaga ttagtcgaga tgtgtatgaa gttctggttg
     5761 tcgcaagaga aaatcatcaa gtgactcaag agcacgtctc aatcttattg acttgcgttc
     5821 aagaattgat ttcagaagtg aatcaagtgc agccactcag tcgtgagttc cgtgaaaaat
     5881 atatgggtta ggaggatgta atggtttatc gctatcgtac caatctcaaa aaagtatttc
     5941 taactggtcc agaattacac caattgaatg aacggattgc taagagcaat tgtcaaaatt
     6001 tctcagtcta tgccagaaaa gttttgctta atcccaatat gtcatttgtc acgattaaca
     6061 ctgatactta tgaccagtta gtttttgaat tgagacggat tggaaataat attaatcaaa
     6121 ttgcgcgcgc tatcaatcag agccatctga tttctcagga acaattacaa gaattgagta
     6181 agggaattag tgagttaatt actggagtag agaaagattt tcaagttgaa gttaatagac
     6241 tgagggaatt ttatggtagt cactaaacac tttgcgacac atggtaaaaa ataccgtaga
     6301 cgtttgatta aatatatcct caatccagaa aaaacggata atctaaaatt agtatctgat
     6361 tttggaatga gtaattactt agactttcct agccatgcag aaatggtaga gatgtacaat
     6421 gtcaacttca ccaataatga caagttgtac gaatccagaa atgaccgaca agaaaaatat
     6481 caacaaac
//
LOCUS       DAVBDG010000088         6452 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBDG010000088 DAVBDG010000000
VERSION     DAVBDG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.089"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            84..1067
                     /locus_tag="VQ526_001898"
     CDS             84..1067
                     /locus_tag="VQ526_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2555576.1"
                     /translation="MFGESFWDEHVKYYYRWARNGSESNDASYAQVVSLYDDTSISVS
                     NNETDKVLTGSLYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAA
                     ETSKIAQFQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPS
                     QTKVVTEELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIY
                     INIMQRSKEIGVMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVS
                     HFYPSITKVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1244..2251
                     /gene="gap"
                     /locus_tag="VQ526_001899"
     CDS             1244..2251
                     /gene="gap"
                     /locus_tag="VQ526_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2555577.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2318..3154)
                     /locus_tag="VQ526_001900"
     CDS             complement(2318..3154)
                     /locus_tag="VQ526_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2555578.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3280..5475
                     /locus_tag="VQ526_001901"
     CDS             3280..5475
                     /locus_tag="VQ526_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2555579.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVDLTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5528..5680
                     /gene="rpmG"
                     /locus_tag="VQ526_001902"
     CDS             5528..5680
                     /gene="rpmG"
                     /locus_tag="VQ526_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2555580.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5690..5866
                     /gene="secE"
                     /locus_tag="VQ526_001903"
     CDS             5690..5866
                     /gene="secE"
                     /locus_tag="VQ526_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2555581.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5921..>6452
                     /gene="nusG"
                     /locus_tag="VQ526_001904"
     CDS             5921..>6452
                     /gene="nusG"
                     /locus_tag="VQ526_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2555582.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2003 a         1127 c         1397 g         1925 t
ORIGIN      
        1 aatacctttt atgaggctct tttgtcctgc tcttgtttca attgactata gttcacggat
       61 acagttggga aagaagctat taaatatttg gagagtcctt ttgggatgaa catgtcaaat
      121 attactatag atgggcaaga aatggatctg agtcaaacga tgcttcttat gctcaagtag
      181 tgagtcttta tgatgataca agtatctctg ttagtaataa tgagacggac aaagtgttga
      241 ctggatccct ctatactgaa acaaatgaac agggattaac gattccaagc agtttactaa
      301 aaaattggaa tgaacagaca ggaaaaaatt tgacaactaa tgatcttatt ggcaaatcag
      361 tctcagccag cattgtagaa agtgctgccg aaactagtaa gattgctcaa tttcaaacga
      421 agattgtacg tgtaatcaat gatgaagatg acatggagga cagcaacagt ttcatgctgt
      481 ctcatcaaat ggaaacgatt ttgaaagagg ctggatttac gaaagctgta tcttatttta
      541 tcttggaact caaagatcca tcacagacaa aagtagtaac agaagaatta cagaaaaata
      601 agaagtatac tgtgctttct caacagagag ttcttgatat tgtgattacc tttattcgtg
      661 ttattcaggg attattgatt gtgctttcat cacaagctat tgtggtagca gcggttatga
      721 ttggtatcat tatttacatc aatatcatgc aacgttccaa ggaaataggt gtcatgaaag
      781 cagttggtta tcagaatcgt ggtgtcaaag gaatttttat ttacgaggct atctggattg
      841 taggcatcgc cttgctgctg gcatttttgg ttgcacaagg ggtgggaagt ttggcgaatg
      901 cgattgtaag tcacttttac ccatccatca ctaaggtttt tgaattaaat cttttatctg
      961 ttttaggaac tctagttttc gctctattac ttggttatgt ctcagcctac ttcccggcgc
     1021 gtaagattag taaaatggat cctgtagaat cgttacgcta tgaataataa gggattcctt
     1081 gatttacagt cttaaaaaga aactcacata gaaaaacaat ggccatactt gcaattcaac
     1141 tcaaatagtt gtataatagg tttgttgaaa ggtgatttgt agtgattcaa gttactcttt
     1201 tcacaataaa aatttcatga ttttcataag gaggaaatca ctaatggtag ttaaagttgg
     1261 tattaacggt ttcggacgta tcggtcgtct tgctttccgt cgtatccaaa acgtagaagg
     1321 tgttgaagtt acacgcatca acgaccttac agatccagtt atgcttgcac acttgttgaa
     1381 atacgacaca actcaaggtc gtttcgacgg tactgttgaa gttaaagaag gtggatttga
     1441 agttaacggt aaattcgtta aagtttctgc tgaacgtgat ccagagcaaa tcgactgggc
     1501 tactgacggt gtagaaatcg ttcttgaagc tactggtttc tttgctaaga aagaagcagc
     1561 tgaaaaacac cttaaaggtg gagctaaaaa agttgttatc actgctcctg gtggaaacga
     1621 cgttaaaaca gttgtattca acactaacca cgacgttctt gacggtactg aaacagttat
     1681 ctcaggtgct tcatgtacta caaactgctt ggctccaatg gctaaagctc ttcaagataa
     1741 ctttggtgtt gttgaaggat tgatgactac tatccacgct tacactggtg accaaatgat
     1801 ccttgacgga ccacaccgtg gtggtgacct tcgccgtgct cgcgctggtg ctgcaaacat
     1861 cgttcctaac tcaactggtg ctgctaaagc tatcggtctt gtaatcccag aattgaacgg
     1921 taaacttgac ggatctgcac aacgcgttcc aactccaact ggatcagtta ctgaattggt
     1981 agcagttctt gaaaagaacg ttactgttga tgaagtgaac gcagctatga aagcagcttc
     2041 aaacgaatca tacggttaca cagaagatcc aatcgtatct tcagatatcg taggtatgtc
     2101 ttacggttca ttgtttgacg caactcaaac taaagttctt gacgttgacg gtaaacaatt
     2161 ggttaaagtt gtatcatggt acgacaacga aatgtcatac actgcacaac ttgttcgtac
     2221 tcttgaatac ttcgcaaaaa ttgctaaata attcttgagt tgatagaaag caaggctttg
     2281 tggtcttgct ttttatatag aaaaatggat gacacgatca tccattcttt tttaattctt
     2341 tttcaaatgt atttgaaagg gtagtgaaag ttagcctctc taaagtaagt gggtgggtaa
     2401 aggaaagtcg gaaggcatga agcataagcc ggcttgtctt tgatttacta ttatagagag
     2461 ggtctcccag gataggaaga ttatgatgca aaaggtgcac acgaatctga tgggttcgcc
     2521 ctgtctttag cttgcaatga gccaaggaag tcttgtttga gaattgcttt aatctgctta
     2581 catgcgtttc agcatattgc ccattttttg catcaactat tctttttcta cgatcatggc
     2641 gatcacgtcc aattttgtct ctgaaaacaa gttcttttct gttgatattt ccatcaacta
     2701 gagcccaata ttctctagaa atctcttttt tctccaataa gcgattgaga atgggcagga
     2761 taaaaggatt tttggcaaag agaactaagc cactggtttc catgtccaga cgatgaacga
     2821 catagcaggt ttggccaaca taggtactga catggttaag aagggcaatt tcgtttggtt
     2881 gattaccatg cgttttcatc ccctctggtt tgtttacaat aatcaagtgt tgatcttgat
     2941 aaacttcctg cactaagtct gggttgcccc aagggatcgt cttttgggga taatcttcct
     3001 cgtcaaaagt caactggcaa acatctccag gatttacgat ttcgttccag tggacttctt
     3061 cttgatttat caaaatatgt ttcttgattc tcaaaaaatg acggattttt ctagggatga
     3121 ggagttgttc ctcaagtaat tgctttaccg tcatttgagg tatagaggcg ggtaatgtaa
     3181 atgtgaattg catacagata ttgtaacaaa aaaagcccta tttggatagg aaatagctaa
     3241 attcttgtct tcctatgatg aagatgataa aataaacgca tgaaattaga taaattattt
     3301 gagaaatttc tttctctttt taaaaaagaa acaagtgaac tagaggactc tgattctact
     3361 atcttacgtc gctctcgtag tgatcgaaaa aaattagccc aagtaggtcc gattcgaaaa
     3421 ttctggcgtc gttatcatct aacaaagatt atccttatac taggtttgag tgcaggcttg
     3481 ctagttggaa tctatttgtt tgctgtagcc aagtcgacca atgtcaatga tttgcaaaat
     3541 gccttgaaaa ctcggactct tatttttgac cgtgaagaaa aagaggctgg tgccttgtct
     3601 ggtcaaaagg gaacctatgt tgatctgact gatatcagta aaaacttgca gaatgctgtt
     3661 attgcgacag aagaccgttc tttctataaa aatgacggga ttaactatgg ccgtttcttc
     3721 ttggctattg tcactgctgg acgttcaggt ggtggctcta ccattaccca acagctggct
     3781 aaaaacgcct atttatcaca ggatcaaact gttgagagaa aagcgaaaga atttttcctt
     3841 gccttagaat taagcaaaaa atatagtaag gagcaaattc taaccatgta ccttaacaac
     3901 gcttattttg gaaatggtgt gtggggtgta gaagatgcga gtaagaaata ctttggagtt
     3961 tctgcatcag aagtgagtct ggatcaagct gcgactctgg cagggatgct caaggggccg
     4021 gaactgtata atcccttgaa ttccgtagaa gattctacta atcggcgcga tactgtcttg
     4081 cagaatatgg ttgcagcagg atatattgat aaaaaccaag aaaccgaagc tgctgaagtt
     4141 gatatgactt cgcaattgca cgataagtat gaaggaaaaa tctcagatta ccgttacccc
     4201 tcttattttg atgcggtggt taatgaagct gtttccaagt ataatctaac agaggaagag
     4261 attgtcaata atggctaccg catttacaca gagctggacc aaaactacca agcaaatatg
     4321 cagattgttt atgaaaacac atcgctattt ccgagggcag aggatggaac gtttgctcaa
     4381 tcaggaagtg tagctctcga accgaaaaca gggggagttc gtggagttgt cggtcaagtt
     4441 gctgacaatg ataaaactgg attccggaat ttcaactatg caacccaatc aaagcgtagt
     4501 cctggttcta caattaagcc tttagttgtt tatacgccag cagttgaagc aggctgggct
     4561 ttgaataagc agttggataa ccataccatg cagtatgaca gctataaggt tgataactat
     4621 gcagggatca aaacgagtcg agaagttcct atgtatcaag ccttggcaga atcgcttaat
     4681 ctacctgctg ttgccactgt taatgatttg ggtgtcgaca aggcttttga ggcaggcgaa
     4741 aaattcggac tcaacatgga aaaggtcgac cgtgttcttg gtgtcgcctt gggaagcggt
     4801 gttgaaacca accctcttca aatggctcaa gcatacgctg cctttgcaaa tgaaggttta
     4861 atgcctgaag ctcattttat tagtagaatt gaaaatgcta gtggacaagt tattgcgaat
     4921 cataaaaatt cacaaaaacg ggtgattgat aagtctgtag ctgacaagat gaccagtatg
     4981 atgttgggga ctttcaccaa cggtaccggt attagttcat cgcctgcaga ctatgtcatg
     5041 gcagggaaaa ctggaacaac tgaagcagtt ttcaatccgg agtacacaag tgaccagtgg
     5101 gtaattggtt atactccgga tgtagtgatt agccactggc ttggctttcc gaccactgat
     5161 gaaaatcact atctagctgg ctctacttca aacggtgcag ctcatgtctt tagaaacatt
     5221 gccaatacca ttttacctta tacgccagga agtaccttta cggttgaaaa tgcttataag
     5281 caaaatggaa ttgcaccagc caatacaaaa agacaagtac aaaccaatga taatagccag
     5341 acagatgata atttgtctga tattcgaggg cgtgcgcaaa gtctagtaga tgaggctagc
     5401 cgggctatct cagatgcgaa gattaaggaa aaggctcaaa caatatggga ttcgatagtc
     5461 aatctatttc gctaagatgc ttgtcaaagc ctagctttct tgttataatg gataagatgg
     5521 aggcgttatg gcactaaaaa aagcaagcct agcttgtgcg gtttgtggtt cgagaaacta
     5581 ttcaatcaag atcagcggaa accccaagcc tacacgacta gaagtaaata aattttgtaa
     5641 gcattgtggc aagtacacta cacacagaga aacgagatag gagagagcga tgcgttttat
     5701 tggagatatt tttagacttc ttaaagacac aacatggcca actcgcaagg aaagctggag
     5761 agattttcgt tctatcatgg aatacacagc tttctttgta gtaattattt acatttttga
     5821 ccagttgatt gtttcaggtt tgattcgatt tattaacatt ttttagaaga ttagtggagt
     5881 taattacact agaaatcttc tatttatgaa aggaaatatc atggatagtt ttgataaagg
     5941 atggtttgtt ttacaaactt attctggtta tgaaaataag gtgaaagaaa atctattaca
     6001 acgtgcacaa acctacaata tgttggataa tattctacgc gttgaaattc caacacaaac
     6061 agtgcaagtt gaaaaaaatg gaaagagaaa agaagtagaa gaaaatcgct ttccaggtta
     6121 tgttcttgta gaaatggtca tgacagatga agcttggttt gttgttcgaa acacaccaaa
     6181 tgttacagga tttgtcggat cacacgggaa cagatcaaaa ccaactccat tattggaaca
     6241 agaaattcgt gacattttgg tatctatggg acaaactgtt caagaatttg atttcgatgt
     6301 tgagattggt caaactgtac gtattattga tggtgctttt gcagactaca ctggtaagat
     6361 tacagaaatt gataataaca aagtgaaaat gattatctct atgtttggta atgacacagt
     6421 tgcagaagta aacctaaacc aaatcgcaga at
//
LOCUS       DAVBDG010000089         6311 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBDG010000089 DAVBDG010000000
VERSION     DAVBDG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.090"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            129..329
                     /locus_tag="VQ526_001905"
     CDS             129..329
                     /locus_tag="VQ526_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555583.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDQK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            371..775
                     /locus_tag="VQ526_001906"
     CDS             371..775
                     /locus_tag="VQ526_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555584.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1006..1713
                     /gene="ccdA2"
                     /locus_tag="VQ526_001907"
     CDS             1006..1713
                     /gene="ccdA2"
                     /locus_tag="VQ526_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2555585.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1724..2281
                     /gene="sdbB"
                     /locus_tag="VQ526_001908"
     CDS             1724..2281
                     /gene="sdbB"
                     /locus_tag="VQ526_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2555586.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDASNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAETAFKEMN"
     gene            2656..4047
                     /locus_tag="VQ526_001909"
     CDS             2656..4047
                     /locus_tag="VQ526_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2555587.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIIAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLILLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4190..5107
                     /gene="adcAII"
                     /locus_tag="VQ526_001910"
     CDS             4190..5107
                     /gene="adcAII"
                     /locus_tag="VQ526_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2555588.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDSDVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLEADPQNDKTYLENLEENMSVLAEELK"
     gene            5115..>6311
                     /locus_tag="VQ526_001911"
     CDS             5115..>6311
                     /locus_tag="VQ526_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2555589.1"
                     /translation="MKINKKYVAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSD
                     LSASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISS
                     LLRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRI
                     ARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPSPQPAPSNPIDEKLVKEAVRKVGDGY
                     VFEENGV"
BASE COUNT         1917 a         1159 c         1343 g         1892 t
ORIGIN      
        1 aaatcgaatt ctaacaatgt tttagaagtc catatgtact attctagttt caatatatca
       61 taactgttcc tagtcaacct gcaagcccta tcccagctcc aagctcagct ccgactcaag
      121 atcctagagt ggtagcaagc gcttcggcta gctcaacgag cacacaagct caagagcaag
      181 ttgacaagtc tgaacttcgt gccttgagtc aagagctaga ccaacgcttg aaagctttgg
      241 caacagtatc tgatcagaaa atcgatgcaa ccaaggctgt cctcctagat gctcaaaaag
      301 ctccggaaga tagtgccttg acagagtaag gtctgcatac tgcagtagag tctgtgaagg
      361 ctgggctgcc ttggactctc tcaaagatgt gaaagcaaat gctagcgaca gcaagcctgc
      421 acaggacaag aaggatgcaa aacaaggaac ggaagatagt aaggattcag ataagatgac
      481 tgaaacaaac tcagttccgg caggagtgat tgtggtcagt ctacttgccc tcctaggcgt
      541 gattgccttc tggctgattc gccgtaagaa agagtcagaa atccagcaat taagcacgga
      601 attgatcaag gttctaggac agctagatgc agaaaaagcg gataaaaaag tccttgccaa
      661 agcccaaaac cttctccaag aaacccttga tttcgtgaaa gaagaaaatg gctcagcaga
      721 gacagaaact aaactagtag aggagcttaa agcaatcctt gacaaactca agtaaaacat
      781 gatttatgaa atgtcagacc taaaagacat tttcgttcac aaaagcagcg atgagtaatc
      841 cttgtcgttg ctttttggct attttggggg ataagatccg ccttgactag caaaagaaaa
      901 gattggtcca atagtgcccg atacgatttt gcgtagcaga aagcttacaa ataaaattga
      961 atatagtaaa ataggattag aattattaag aaagttggtt ctttattgga aacgatagta
     1021 tttttaatct ctgtttttct agcaggtgtt ttatcctttt tttctccttg tatttttcct
     1081 cttctgccag tctatgctgg gattttattg gatgatcaag aaagtgcaaa aagcttttct
     1141 ttgtttggga gaaaggttct ctggtcaggc ttgattcgaa cactttgctt tatcgctggt
     1201 atctctctca ttttctttat tctaggcttt ggtgctggtt actttggtca tattctctat
     1261 gcaaattggt ttcgatatgg catgggagct attattatca ttttgggtct tcaccagatg
     1321 gaaatttttc atttgaagaa attagaagtt caaaaaagtt ttacctttaa aaaatcagat
     1381 tctaatcgtt attggtcagc ttttttactt ggtattacct ttagctttgg ttggacacct
     1441 tgtattggtc cagttttaag ttctgtttta gcacttgcgg cttctggagg caatggcgct
     1501 tggcaaggag cgatttatac tctcatttac actctgggca tggcccttcc tttcttggta
     1561 ttggcactag cttcaggtct agtcatgcca tattttagta aaatcaagcg tcatatgatg
     1621 ctactaaaga aaattggtgg tttcctcatt gttttaatgg gaattttgtt actattagga
     1681 caagtaaatg ttctagctgg aatttttgaa taaaggagga caaatgaaaa aagtaatgtt
     1741 tgctggctta agtctcttgt cattagttgt attgatggcc tgtggtgagg aagaaactaa
     1801 aaagactcaa gcagcacaac agccaaaaca acaaacgact gtacaacaaa ttgctgttgg
     1861 aaaagatgct tcaaacttca cattgcaatc catggatggc aaagaagtta agttatctga
     1921 ttttaagggt aaaaaggttt acttgaagtt ttgggcttca tggtgcggtc catgcaagaa
     1981 aagtatgcca gagttgatgg aactagcggc gaaaccagat cgtgatttcg aaattcttac
     2041 tgtcattgca ccaggaattc aaggtgaaaa aactgttgag caattcccac aatggttcca
     2101 ggaacaagga tataaggata tcccagttct ttatgatacc aaagcaacca ccttccaagc
     2161 ttatcaaatt cgaagcattc ctacagaata tttaattgat agccaaggaa agattggaaa
     2221 gattcaattt ggtgctatca gtaatgcgga tgcagaaaca gcatttaaag aaatgaacta
     2281 gaatcgataa aatctgatta caagatgtag tcagattttg gtaacttcta aaactaactt
     2341 ccagtttccc acaacctagc ttttagtatg agaaaaacgc gtgacatcag tggaaatccg
     2401 tcttagacta acttccactg gttttctttt tcttgatata taagggttca aaagcgaaaa
     2461 gactcagaaa aaagtgcacg acaaatagcc ctaaaacaga gtcgttttag agtaacttcc
     2521 agttttggga gagagatgga agttactttg agaagttacg gtagtcagat ttttttagaa
     2581 aaacatttta taaatattca catatctcct atatttatgg taaaatagaa tcatcagttt
     2641 attttggagt caaagatgaa tatatttaga acaaagaatg ttagtttaga taaaacagag
     2701 atgcataggc atttgaagtt atgggatttg attttgctgg gtattggagc catggtaggg
     2761 acaggtgtct ttacaatcac aggtactgca gctgctacac ttgctggtcc agcactagtg
     2821 atttcaatcg ttatttctgc cttgtgtgtg ggattatcag ccctcttttt tgcagaattt
     2881 gcctcgcgag tacccgctac aggaggtgcc tatagttacc tctatgctat cttaggagaa
     2941 ttccctgcct ggttggctgg ttggttaacc atgatggagt tcatgacagc catatcaggc
     3001 gtagcttcgg gttgggcagc ttattttaaa ggcttgctct ctcaatacgg gatagccctt
     3061 cctcaggctt taaatggtac ctttaatcct caagcaggga catttgttga tttattgcct
     3121 attcttgtct tggtcttggt gacttcgctt gttttactaa atgccaaggc agccttacgc
     3181 tttaattcga ttctagttat tttgaaattt tccgctttag ctctctttgt tttagtagga
     3241 atttggaata ttaagtttga taattggagc aattttgctc cttacggttt tggacaaatc
     3301 tatggagcta gtactggtat tatagctggt gcgtccttga tgttcttcgg ttttttggga
     3361 tttgaatcca tctctatggc tgtagatgaa gtcaagactc ctcaaaaaaa tattcctaga
     3421 gggattgtct tatcgctttc tatcgtaacc attctctatg ccttggtgac ccttgttttg
     3481 actggtgtgg ttcactatag tcatttaaat gtcgatgatg ccgttgcctt tgcccttcgt
     3541 agtgttggga ttagttgggc agccaactat gtgtcattag tggctatctt gaccttgatt
     3601 acagtttgta tttcgatgac ctatgcccta tcgcgtatga tttacagttt agcacgtgat
     3661 ggcttagtgc ctgctgcctt taaggaactg acgaagacta gcaagatacc aaagaatgct
     3721 actatcttga caggtctagc ttcagcagta gcagcaggaa tgttcccgct agccagtatt
     3781 gcagcctttt taaatatttg taccttagcc tacttgatcc tgctggctta tggtctgatt
     3841 cgcttacgga aagaaaaagg aatgcccaaa gctggggaat ttaaaacacc attggtacct
     3901 ctgttaccaa ttttatcgat catcatttgt ttatccttca tgttacagta taatatgaat
     3961 acctggattg ctttcctagt tgcgttgtta gtaggaagta ttatttactt cacttatggc
     4021 tataagcatt ctaccataga agaataaagt gaaagtctat tgagttgttg acactcagta
     4081 gatttttata tgtgaaagaa atttcaaatt ttttctaaaa atatcttgac agattaaatt
     4141 ttcaggagta gaatatttac tagttaatta aatggttaag gagttgttca tgaagaaaca
     4201 aaatttattt ttagtcctgt taagtgtctt tcttttgtgc ttgggggctt gtggtcaaaa
     4261 ggaaagtcag acaggaaagg ggatgaaaat tgtgaccagt ttttatccta tctacgctat
     4321 ggttaaggaa gtatctggtg acttgaatga tgttcggatg attcagtcaa gtagtggtat
     4381 tcactccttt gaaccttcgg caaatgatat cgcagccatc tatgattcag atgtctttgt
     4441 ttaccattct catacactcg aatcttgggc aggaagtctg gatccaaatc taaaaaaatc
     4501 caaagtgaag gtcttagagg cttctgaggg aatgaccttg gaacgtgtcc ctggactaga
     4561 ggatgtggaa gcaggggatg gagttgatga aaaaacgctc tatgaccctc acacatggct
     4621 agatcctgaa aaagctggag aagaagccca aattatcgct gataaacttt cagaggtgga
     4681 tagtgagcat aaagagactt atcagaaaaa tgcgcaagcc tttatcaaaa aagctcagga
     4741 attgactaag aaattccaac caaaatttga aaaagcgact cagaaaacat ttgtaacaca
     4801 acatacagcc ttttcttatc tagcgaagag atttgggctt aatcaacttg gtattgcagg
     4861 catctctcct gaacaagaac caagtccacg acaattaaca gaaattcagg aatttgttaa
     4921 aacctataag gttaaaacga tttttacaga aagtaatgct tcttcaaaag tagctgaaac
     4981 tcttgtcaaa tcaacaggtg tgggtcttaa aactctgaat cctttagagg cagacccaca
     5041 aaatgacaag acctatttag aaaatctgga agaaaatatg agtgttctag cagaagaatt
     5101 aaagtgagga aagaatgaaa atcaataaaa aatatgtagc aggttcagtg gcagttcttg
     5161 ccctaagtgt ttgttcctat gaacttgggc gttaccaagc tggtcaggat aagaaagagt
     5221 ctaatcgagt tgcttatata gatggtgatc aggctggtca aaaggcagaa aatttgacac
     5281 cagatgaagt cagtaagagg gaggggatca acgccgaaca aatcgtcatc aagattacgg
     5341 atcaaggtta tgtgacctct catggagacc attatcatta ctataatggc aaggtccctt
     5401 atgatgccat catcagtgaa gagctcctca tgaaagatcc gaattatcag ttgaaggatt
     5461 cagacattgt caatgaaatc aagggtggtt atgtcattaa ggtaaacggt aaatactatg
     5521 tttaccttaa ggatgcagct catgcggata atattcggac aaaagaagag attaaacgtc
     5581 agaagcagga acacagtcat aatcacgggg gtggttctaa cgatcaagca gtagttgcag
     5641 ccagagccca aggacgctac accacagatg atgggtatat cttcaatgca tctgatatca
     5701 ttgaggacac gggtgatgct tatattgtcc ctcacggcaa tcacttccac tatattccta
     5761 agagtgattt gtctgccagt gaattagctg ctgcccaagc ctattggaat gggaagcagg
     5821 gatctcgtcc ttcttcaagt tctagtcata atgcaaatcc agctcaacca agattgtcag
     5881 agaaccacaa tctgactgtc actccaactt atcatcaaaa tcaaggggaa aacatttcaa
     5941 gccttttacg tgaattgtat gctaaaccct tatcagaacg ccatgtggaa tctgatggcc
     6001 ttattttcga cccagcgcaa atcacaagtc gaaccgccag aggtgtagct gtccctcatg
     6061 gtaaccatta ccactttatc ccttatgaac aaatgtctga attggaaaaa cgaattgctc
     6121 gtattattcc ccttcgttat cgttcaaacc attgggtacc agattcaaga ccagaacaac
     6181 caagtccaca accgactcca gaacctagtc caagtccgca accagctcca agcaatccaa
     6241 ttgatgagaa attggtcaaa gaagctgttc gaaaagtagg cgatggttat gtctttgagg
     6301 agaatggagt t
//
LOCUS       DAVBDG010000090         5652 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBDG010000090 DAVBDG010000000
VERSION     DAVBDG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.091"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..1172)
                     /locus_tag="VQ526_001912"
     CDS             complement(<1..1172)
                     /locus_tag="VQ526_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2555590.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVASQSKAEK
                     DYDAAKRDAENAKKALEEAKRAQKKYEDDQKKTEEKAKKEKEASKEEQAANLKYQQEL
                     VKYASEKDSVKKAKILKEVEEAEKEHKKKRAEFEKVRSEVIPSAEELKKTRQKAEEAK
                     AKEAELIKKVEEAEKKVTEAKQKLDAERAKEVALQAKIAELENEVYRLETELKGIDES
                     DSEDYVKEGLRAPLQSELDAKRTKLSTLEELSDKIDELDAEIAKLEKNVEYFKKTDAE
                     QTEQYLAAAEKDLADKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPAPAPKPEKTDDQQAEEDYARRSEEEYNRLPQQQPPKAEKPAPAPKPEQPVPA"
     gene            complement(1722..1961)
                     /locus_tag="VQ526_001913"
     CDS             complement(1722..1961)
                     /locus_tag="VQ526_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555591.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     DLHKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            complement(3092..3820)
                     /locus_tag="VQ526_001914"
     CDS             complement(3092..3820)
                     /locus_tag="VQ526_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555592.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFITELIIFHL
                     IGKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICADTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEFELFKNSK"
     gene            complement(3817..4170)
                     /locus_tag="VQ526_001915"
     CDS             complement(3817..4170)
                     /locus_tag="VQ526_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2555593.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGEAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYYIGYGVLNRGCDIN
                     GNPYPWDGFISATVK"
     gene            complement(4226..5128)
                     /locus_tag="VQ526_001916"
     CDS             complement(4226..5128)
                     /locus_tag="VQ526_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555594.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESDLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(5336..>5652)
                     /locus_tag="VQ526_001917"
     CDS             complement(5336..>5652)
                     /locus_tag="VQ526_001917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET2555595.1"
                     /translation="DGGFSIPRWPVATAINIAFNDVLGGGAISLVRNYIRNYGLRRVT
                     SAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGNAITTIWANNDVNRRDPNLNA
                     LW"
BASE COUNT         1811 a         1077 c          785 g         1979 t
ORIGIN      
        1 gcaggaactg gttgctctgg ttttggtgct ggagctggtt tttctgcttt tggcggttgc
       61 tgttggggca agcggttata ttcttcttct gatctacgag catagtcttc ttcagcttgt
      121 tgatcatctg ttttttctgg ttttggtgct ggagctggtt gaggagccgg cgctggtttt
      181 ggttgttcag ctggttgctc tggttttggt gctggagctg gagtttcttc agctggtttt
      241 tctggctcat taactgcttt cttaaggtca gcttcagttt tctccaattc agctttttta
      301 tcagctaagt ctttttcagc tgcagcaagg tattgttcag tttgctcagc atcggttttt
      361 ttgaaatatt ctacattttt ttcaagtttt gcaatttcag cgtctaactc atcaatctta
      421 tcactcaact cttcaagtgt tgatagttta gttcgtttgg catccaattc agattgaaga
      481 ggagcacgga gaccttcttt aacataatct tctgagtcag attcatcaat ccctttgagt
      541 tctgtttcta gtctataaac ttcattttcc aactcagcga ttttggcttg aagagcaact
      601 tctttagcac gttcagcatc caatttttgt ttggcttcag taactttttt ctcagcttct
      661 tctacttttt taataagttc tgcttctttt gctttagcct cttctgcttt ttgtctagtc
      721 ttttttaact cttccgcgct aggaattacc tctgatctaa ctttctcaaa ttctgctcgt
      781 tttttcttat gctctttctc agcttcctcc acttctttca gaattttagc tttttttact
      841 gaatcttttt cactagcata tttaaccaac tcttgttgat atttcagatt tgcagcttgt
      901 tcctctttag aagcttcttt ctcttttttc gctttctcct cagttttctt ctgatcatcc
      961 tcatattttt tctgcgcacg ttttgcttct tctaaagctt ttttcgcatt ctcagcgtct
     1021 ctcttcgctg catcatagtc tttttcagct ttagactgac tagctacggg ggcttcttct
     1081 gctcttacaa cagtaggctg agacgcaaca aaaccagccc ctaagatagc gacgctggct
     1141 agacttgtta aaatcatttt tttcttattc atctaaattt acctcttttc tgatagtcat
     1201 ttttagacta tacttatatt aagtatactc cccccgtaaa tatttgtcaa atgttttata
     1261 ttacattttt attacaaatt tctatatcat aagcttctac tccaaaaaga taaagacact
     1321 acttttgccc ttttcgattc tctttttaaa catagcatca tcttccttca ttcctataaa
     1381 aacatttccg atgagagagc ttgtttttta atttcgttaa acaacaaaaa acgcaagctt
     1441 aagcctgcgg acaatcttta tagccatttc atttctttca catctgtttt acgaacttta
     1501 gcatatttct tagcgcttgt aaaatgtaga gtaactggtt taaatactcc gcttttgtct
     1561 cttcgtaaac ctaaaactgc ggatctttta tttttatcct caaaaaatat gacgtccaat
     1621 ttcatagtat ttttagataa atccttagct acgatgcaac aacttgctag cttagatata
     1681 gaagatatcg ccgataaact cacataaaga tatcctttcg attacatcat tgtacatagg
     1741 atatttctaa aatcttctaa aataaaaaca ccagcttgaa tagcaggttt tgtttgacta
     1801 gcataatctc gtgtgatttt gtgtaagtca aataaatgat gtaaatcagt caagcaaaaa
     1861 ttcactttga aagtctctaa atacttataa ttcgttgata cttgacatgt ttttccattg
     1921 aatctttctt gataatccaa aatacaatca agtaatttca acgacaaact ccatattttc
     1981 tcaaaataaa aagcacgggg cagttctcac tactgcccaa agtggttgat tagagtcggc
     2041 ttctctaact ccctatgttg tgcttattag gcttatcgca agggaacagt ttttagatct
     2101 gccaagccac atagtgtgct tactactatg tgtccatgtc accatgaatt gccgaacggt
     2161 ttaacgagaa agtatagtag attgaaacaa gatatgaaca aatcgattag gaaagtcaaa
     2221 ttaatttcta gaaatatttt aacagtcata acggactatt ccagattcaa tctactatat
     2281 tatttttaga tttatcccaa ctcaattatg acatcttttt tcaaaagtca atatatctca
     2341 ctcttttcaa cgacaagaaa gaggctgata atcgaccaac ctctgttttt agatacagtt
     2401 caagtccaaa ctattcttta tagaaaagtc cgataaattc gagcaatagt taacatactt
     2461 acaaatataa ctcccaaaat agataacact attttcacaa aactcagttc cgaacctatg
     2521 acatatatat gaaaaagagg agctagtcct acacatatat acaataagta gccaacaagt
     2581 tttatctcac caattcctgc aaaaaaatgt ttcatgtaac gattctccat ttactctatt
     2641 aaatcagttt tattttagac tatcataaaa caatcacctc ttcaataaaa accattggcg
     2701 aatactattc tgaagcattg atagctcaga tttctcagaa tagagctatc ctaactatcg
     2761 agcagctcaa atacatatat atgagcaatt ctaagcctag aattgctata aaataaatgt
     2821 tttcgggcta ttctaagtct ttgactgctt aaaattttac attctgaagg attctaaacc
     2881 tagaatagct caaaatcacg agaactaagg tattcgaaac ttcagagtaa tacaatcccc
     2941 ataggaattc tccattctta ttaagcatga ttaaaatgta atctaacaag tgatcctcga
     3001 ttgtgtttgt agcacttagt tacctagata agactatttc cctttatcat atctgatttt
     3061 aagccgttct aagatttaac ctatgtttct attacttgct atttttaaat aattcgaatt
     3121 ccttttcttt gataaatgga actgaaaaat tagaaaaaag aagtaaaacc gaaaacaaat
     3181 agcctgcttt ttgaatcact aaaagatatc ctaaaaataa gtaagctata acatccaatg
     3241 cgaaaagaaa aaatattctc ctcttcatta aagtacttgt gtcaaataat ctgatgaata
     3301 aaaattttat cgtggtatct gcacaaattt ttatcaaaaa gataagagtc agtacatgat
     3361 tatcaactgt accataaaga atagatggaa atatagttaa tataaaacta gaaatctcta
     3421 aatattttat ccaaggagat tgagacacct tgcttctaaa ctgactaatt ttcttatatt
     3481 cgttagtcaa tttaatatca aaatctttcc caattaaatg aaaaattatc aactctgtaa
     3541 taaaaacact tattaatacg gtataagtca aaaaactttc tgggaaaaac ttattgatta
     3601 aaatggggaa actcattaac agaatcgaat ttatggctag acagatagat actattttat
     3661 ttttcacaga atatagaggt agtgatacta ttgagtagat aaaagctaaa aaaataagca
     3721 aaaaatactc cgtgtcattt ataatcgccc tgaatgataa aaatatatac atccaacata
     3781 cgataatgtg tatttttcga atcttatcta cattactcat tttactgtcg cagatatgaa
     3841 tccatcccaa ggataagggt tcccgttaat atcacaacct ctattaagaa caccatatcc
     3901 aatatagtaa gctattttcc ctgctgctgt agccaataca cctgctgctg caccaataag
     3961 ccagcctcca agacctccaa ccttaatata gtttattagt tttttggtta aaactgcagc
     4021 tccagctaat gctataaaag ttcctgaact catagctaaa gctgcgcaaa ttccttgaca
     4081 agcacgcttg cttaaataag cttccccatc aagatacatc atttcttctt catcaatcac
     4141 aacataatta tttggtaata caagttccat ttgttttctc ctccttagtt gaaattgttt
     4201 catttgtttt acaaatagga cgaacttaaa ctatcttttt attactcagt aataatttcc
     4261 gagatttcat tttatgatac tctctattca tacagaaatc aaaattttgc cttaaccaac
     4321 ttgtataata gaaaaaatct aattgattga ttctgatgag ctgtattcta attttatcaa
     4381 ccttttcttg ccagttgtta ttgttactaa tagaaatttc atacagcagt aactcgacaa
     4441 gacctgtaca cagaagggtg ttatgagaat cttcgatttt tttcaaaata tcctcaaaaa
     4501 gttcactatc cgttaaaaag gtggattggt tcatcgcatc atacagctga agagacatta
     4561 agtcagaata acttagctct gatttttgta tcttatcctt taacgttttc gtcaaatcac
     4621 tttcaaacga atcttctttt tgccatttgt tttgattaaa aagaaattcc gtcttattaa
     4681 aaacgagatg attgatatcc attatagtcc agaaaaatat tgtagtaagc atgatgattg
     4741 caaaaggaat ttttaaaata aatactagga taatagcaat ttcaagaata aaactccata
     4801 taatcctaga tcttaaatca tcaggcgaca aacttttacg atggaatgaa aaatagggcg
     4861 agaacctcac atttactttc ttcctgccta taaaaataaa aggaaaaatc gtaataagag
     4921 cttctttctt gtacaaatct ttcaatgcgt atagtatgtc taaaactatt tttgataaaa
     4981 tcaaactaca gaaagcaaga ctcatactaa tcacaaaatc aaggggagat tctagattca
     5041 atagtaagtt attcactagg acatgcaact ctagccacaa gacaaaaaga gggagcatta
     5101 ataaaaaaat gattagttta cgtatcataa ttttacagtg tccttatcat tttcttaatt
     5161 ttggataaat tatcattgct atgattaaaa tcaaaaaaac gacattcatc caaataatca
     5221 acttttttat ttcttcgcta acaaaatcat aggctaaaaa agttatggat gatataaata
     5281 gacatacaaa aaaaataata acaaaaattt tttccatttt aatacccata tctagttacc
     5341 acaaggcgtt taaatttggg tctctcctat ttacatcatt attggcccag attgttgtaa
     5401 tagcattgcc aattgacatc caagctgcaa atccattaat agcagacagt gcaaatcctg
     5461 ccactctatt tgcaactcgt accccaacat atcttgcagc tgctccagca attgcgcttg
     5521 taactcgccg caaaccataa ttacgaatat aatttctaac tagactgatt gctcctccac
     5581 ctaagacatc attaaaagct atattaatgg ctgttgcaac aggccatctc ggaatagaaa
     5641 atcccccatc aa
//
LOCUS       DAVBDG010000091         5503 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBDG010000091 DAVBDG010000000
VERSION     DAVBDG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.092"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..189)
                     /locus_tag="VQ526_001918"
     CDS             complement(<1..189)
                     /locus_tag="VQ526_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555596.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTL"
     gene            complement(649..1377)
                     /locus_tag="VQ526_001919"
     CDS             complement(649..1377)
                     /locus_tag="VQ526_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2555597.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1535..2944
                     /locus_tag="VQ526_001920"
     CDS             1535..2944
                     /locus_tag="VQ526_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2555598.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2962..4257
                     /locus_tag="VQ526_001921"
     CDS             2962..4257
                     /locus_tag="VQ526_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2555599.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVNRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4262..4570
                     /locus_tag="VQ526_001922"
     CDS             4262..4570
                     /locus_tag="VQ526_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2555600.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4567..4875
                     /locus_tag="VQ526_001923"
     CDS             4567..4875
                     /locus_tag="VQ526_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2555601.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1867 a          925 c          971 g         1740 t
ORIGIN      
        1 taaagttgac tttcctgtac catttttacc agtaattaat gtcacgcctg aactcgaagt
       61 atcaaaagaa atattatcaa acaatatacg atgatccacc ctatacgaaa tattttctac
      121 tttcattttt tctccttttg aactaatagc aaaagaaaca gttctgtttt catactcttt
      181 ccccttcatc tatattattc gtaatgaaaa ataaaaaaat aaaatttttt taaatgatta
      241 tgattcagaa tttattcaat atattaaaac aatatttgaa caaataaatt gggagattga
      301 aattcatttc tagcaatttg ttagaagtca cagtctacta ttctaacctc agtatactat
      361 atgtgttaca agaaaagact caaaaacttc tcgtcagtgt aagaatattt gaaacactat
      421 ttaaaactaa ataactataa gaagtagatc aggtgaacaa tttcagtacc tactctataa
      481 gctatacgaa gatatttttc aacatgatgg ataaaacgat cactctttta aagccatctt
      541 atttagaaaa gagaaaccat ttaagcatag atgggacctt cttgccctgt tttaaatttt
      601 tcttaaacta gagtctgtaa aagattggct tcattttcac attttctcct aggaggaatg
      661 tcgtaatgca aaagaattaa attcaaataa atcatagcga tgacgactaa tcgagtactc
      721 aaacggagtt ccgttatcca aataagccac ttgttctact tcaaataccg gttccgttgg
      781 aaggagctgc aagtaatgtt gctcattttc tgaactagaa gaagctcgta agatttttgt
      841 agcactggca atatgcaatc ctaactttcc ttcaatgtaa ttgtaaatcg atttttgtaa
      901 aatatcttca gtaatacctg gaataacatc ggtactcata taagtttgtt ccagaacata
      961 aggtttaccg tcaataatac gcaggcggta aatattataa actggactct gcaaagcgac
     1021 ctgtagtttt tctgctaaaa attcatttgc aaattctaat ttgaagtgaa taatcctcga
     1081 gtcaattgta gaatgagcat cttcagatat ttttgtcagt ccccggatat ctctttctgg
     1141 aacgattaat tttctttttg agctgccacg agagagaaca aaggttccct gcccacgttt
     1201 tctaaaaatt aagccctcag aaactaacaa gtctaaagct tttttgatcg tcattcgact
     1261 acaggcaaac tgtttagcta atgtcatttc atcaggtaat tgttcgttca tggtatattt
     1321 attacaaaga atcttttcac gaatctcatt gtaaataaat aaatattttg tactcattta
     1381 atctggttct ctctataata ttctaatagt agtataacaa agttcaagaa atgaggcaaa
     1441 cacaagatat gtatcaacaa tttttaaaaa tgtctagaca aattttaaaa ttatgctata
     1501 ctttatgtat agaataatag aaaggaggtt gagcatgaaa aaaaagttag tatttcctaa
     1561 tctgttttgg tggggagcag cttctagcgg acctcagaca gaaggtcaat atggaaaagt
     1621 acatgaaaat gtgatggact actggttcaa aacgcatcca gaagattttt tcgataatgt
     1681 cggacctctt gtagccagta acttttttca tacttacacc gaagatttcc acttgatgaa
     1741 ggaaattgga gttaattctt tccgcacttc catccaatgg agtcgactca tcaagaattt
     1801 agagacaggt gagcctgatc caaaaggtat tgctttctac aatgccatca ttgaagaagc
     1861 taaaaagaac cagatggatc ttgtgatgaa tttacatcat tttgatttac cagtggaact
     1921 tcttcaaaaa tacggtggtt gggaaagcaa acatgtagtg gagttattcg tgaagtttgc
     1981 caagactgct ttcacatgct ttggagataa ggttcattac tggacaactt tcaatgagcc
     2041 aatggtcatt ccagaagcag gatacttata tgctttccat tatccaaatc taaaaggaaa
     2101 gggaaaagag gccgtacaag tcatctataa tctaaacctt gctagtgcaa aagtgattca
     2161 actatatcgc tcattagaac ttgatggaaa gattgggatt attttaaact tgacacctgc
     2221 ttatccaaga agtaattctc cagaagactt agaagcaagt cgatttacag atgacttctt
     2281 taacaaagtc ttcttgaatc cagctgttaa aggaactttc ccagaaagat tggtaaaaca
     2341 gctagagaga gatggcgtgt tatggagtca taccgaaaaa gagcttcaac tgatgaaatc
     2401 aaatacggtt gattttcttg gagtaaacta ctaccatcca aaacgtgttc aagcacaagc
     2461 aaatcctgag gaatatcaga cgccctggat gccagaccaa tacttcaaag agtatgaatg
     2521 gccggagcgt cgcatgaatc catatcgtgg ttgggaaatt tttccgaaat ccatttatga
     2581 tattgctatg attgtgaagg aagaatatgg taatatccca tggtttatca gtgaaaacgg
     2641 aatgggtgtt gaaaacgaag cacggtttat cgatgaaaat ggagttatcg atgacgtgta
     2701 tcgtattgaa ttttatgaag aacatttaag atggctacat aaagccattg aagagggaag
     2761 tcactgtttt ggataccacg cttggaccgc atttgattgc tggtcttgga ataatgcata
     2821 taagaatcgt tacggattta tctccgttga tttagaaacg caaaagagaa ccatcaagag
     2881 ctcaggaaga tggtatcgca aagtaagtga caataacggt tttgaagtag aaattgagga
     2941 gtaaagagag gagatattca tatgaaaaac tcaaaattta tagaccaatt tgccaccttt
     3001 gctggtaaac tagggaacca aattcattta aaaaccctaa gagatgcatt cgtaacagta
     3061 atgccattat atattttggc aggtttaatc gttcttttga acaacacggt atttaagtgg
     3121 attttccaag gggatacatt aacaagattc caatattggg gaataacaat tgcaaacggt
     3181 actttaagta tttcaggtat gattattgct gtaatggttg gttatttctt agctaaaaac
     3241 agagatttcg aaaacccgtt agcagcatca atgctatcat tagtttcttt aattgtgatg
     3301 atgccaaata cagtttctgt agttcctgac ggagcaaaag atgctgtaaa catttcaggt
     3361 gttctttcat tcaacaacac aggtacaggc gcaatgttcg ccggggttat cgtagcgatt
     3421 attgcaacag aattattcat tgaattatca aatgttaaag ctttacaaat gaaccttggt
     3481 gaaaatattc caccagctgt taatagatca tttagcgtat tacttccagt catgaccgtc
     3541 atctccttat ttggggttgt ttcagcatta ttattcaata taactggaat gaacttaatc
     3601 tcaatcatta caatctttat tcaagaacca attcgtcata ttggtacaag cttaatcggg
     3661 gtcattatta tttactcttt aggaaatatg ttatggctat ttggtattca ccaagcagtt
     3721 atttacagtg ccatcctaga accattacta ttaattaaca ttactgaaaa catcactgca
     3781 gcaaataatg gacaagccat tccacacatc atcaacctat cacaaataca aacattcgct
     3841 ttaatgggtg gtagtggatc tacattatgt ttattaatag caacattctt agtgagtcgc
     3901 aatgctgtct ctaaaaacgt ggctaaatta tcttttggac ctggtatctt caatatcaat
     3961 gaaccagtat tattcggtta cccaatcgtt tataacattt cattagctat tccatttatc
     4021 acagttccag tccttggtat tttaatcagc tacttagcaa cagttacagg attcatgagt
     4081 cctgcattta tacaagttcc ttggactaca ccagtattct taaatgcatg gttagcaaca
     4141 gcaggggacg tgagagcagt tctagttcaa ttcatcatct ttgcacttgg agttcttcta
     4201 tacattccat ttatcaaagt taatgacaaa gttgttgaac aagaaatgga aggttaagat
     4261 tatgaaacaa attttattag tatgtaatgc gggaatgtcg acaagtatgc ttgttaaaaa
     4321 aatgcaacaa agtgcgactg aacgtgggat tgaaatctct atccaagcaa aatcaatgac
     4381 agaagctaaa aagaatatcc atgaagcaga tgtcattcta attgggccac aaattcgtta
     4441 tgagttactt gctgtgaaag aaattgcagg aaatatccct gtagatacca ttgatatgcg
     4501 ggattacgga atgatgaatg gagcaaaagt tctggaacaa gcacttgcat ggataggaga
     4561 aataagatga cagaagaaat ggaatatatc tgttttcagc tcattgccaa tagtggagca
     4621 gccaaatcat cctttattga agccatccaa ctagctaagg caggaaattt aaaagaagcg
     4681 aaaattaaag ttgaagaagc ggaagactcc ttagtagaag cgcacaagat tcactctaat
     4741 ctcatacaaa aagaagctac tggagaaaaa ataggattct ccttgctatt tatgcatgca
     4801 gaagaccaaa tggcttcaac agagattatt cagttgctat ctaaagaatt catcgatctc
     4861 tatcaaaata aataggaaca ctgtcattcc catctgtctt tattaatgac gactacattg
     4921 taggaacctc caaaagttgg acaaaatgat tgaaaggatt ggatcctgca ttacacaaga
     4981 tccaatcctt ttcttttact ctagttttta taatatgttg aatctagaat agtccactgt
     5041 agtttctaaa aaattgctgg aaatggatgt taagctccaa tttatttgtt tatatcttat
     5101 ttcagtttac tatactagct tattattcta gtgaaaaatt gaatgagcca ctatttattt
     5161 aatatgtatg atgagggaat gactcaaaaa cttcttgtcg atataagaat atctgagata
     5221 gtattttaaa acgaaaggac tataaaagct catttccttc gcacttattt tatagagatc
     5281 tatcattata ctctttagaa atcaaaaagc aacacacctt gtcatcgttt tatcgtaaat
     5341 ctgttaatga gggggctagt cttatctgca atctcaaatc agtgttttga gctgaatggc
     5401 actcgttttc tagtttgctc tttgatttta attgggcctt agtaaatact actcttaaat
     5461 taaactatag tgaattgaaa taagatgtga acaactctat cag
//
LOCUS       DAVBDG010000092         5218 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBDG010000092 DAVBDG010000000
VERSION     DAVBDG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.093"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..84
                     /locus_tag="VQ526_001924"
     CDS             <1..84
                     /locus_tag="VQ526_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET2555602.1"
                     /translation="LLELTEETSMLSHLKKEVETHYQPKKL"
     gene            complement(374..844)
                     /locus_tag="VQ526_001925"
     CDS             complement(374..844)
                     /locus_tag="VQ526_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET2555603.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(810..1616)
                     /locus_tag="VQ526_001926"
     CDS             complement(810..1616)
                     /locus_tag="VQ526_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2555604.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(1823..2464)
                     /locus_tag="VQ526_001927"
     CDS             complement(1823..2464)
                     /locus_tag="VQ526_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555605.1"
                     /translation="MIELENITKTIGGKVILDHLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSSTLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(2461..4569)
                     /locus_tag="VQ526_001928"
     CDS             complement(2461..4569)
                     /locus_tag="VQ526_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2555606.1"
                     /translation="MKKISHLCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     VLASRMEKRSLVELLKGGIL"
     gene            complement(4624..4908)
                     /locus_tag="VQ526_001929"
     CDS             complement(4624..4908)
                     /locus_tag="VQ526_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2555607.1"
                     /translation="MRKLVKIGVASLMVCSILASTSVLAVWVDGGQWNYGVGWSGNFG
                     YSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
BASE COUNT         1624 a         1158 c          836 g         1600 t
ORIGIN      
        1 ctcctagagc tgaccgagga aacttctatg ctgtctcatc tcaaaaaaga agtagagacc
       61 cattatcaac caaagaaatt ataaaaaaag tcgagggatt tcctcgactc tttacatatt
      121 atttgttcgt tagttcttaa taccagccat tgttaagcca gaagttttta gctctgccaa
      181 gccacatagt gtgtttacta ctatgtgtcc atgtcaccat gaatcgccga acgatttaac
      241 gagaaagtat gacttttacg tttatcccaa ctcaattatg acattttttt caaaagtcaa
      301 tatatctcac tttttcaacg acaagaaaga ggctaccaat cgaccaacct cttattctga
      361 acccatcact ccatcacttt ttagcttcat tcgctttctt agcgactgca atctggtatt
      421 cgacttggtc attcccctta ccggtacagc catgagcaat tgtagtcgct cctgtctgat
      481 gcgctatttc aactaatttt ttagaaataa gagggcggct caaggcagat accaaggggt
      541 acttttgttc ataataggca tgtgactgaa gagccactag cacataatct gtagcaaatt
      601 cgtccttgac atcaatgaca taagattcga ctgccccaac cttgagagcc ttatcatgga
      661 tgaagtctaa gtctttccct tcacccacat ccatacaaac tgaaacaaca tcatagtctt
      721 tctttaacca ggtaatagca actgatgtgt ccaaaccgcc agaataggcc aaaattactt
      781 tttctttact catgttcttt tccttctttc tatttttcaa tggatgcttt aaaggcatcc
      841 tcaatgagtt tgtctaattc cccagactcc ttcaactttt gaatggtttt atcgactgcc
      901 tctttcaatt ccttgctatc ttttttcatg gctaccgcgt aggaatcatc ttgctctttt
      961 tcaaaattga ggtctgcgat tgctaaatca ggattatttt ccacaaatcc cttggaaaca
     1021 ggttcttcaa agataacggc atccacttgt cctgatttta aatctgtgat taaattccca
     1081 tttttaggca gagatacgag ggaagaattt tgtagcaaat ctttcgccat cgtctcttga
     1141 atcgaacctt tctgcgctcc aacctttttc tgcgccaagt cgtttacaga ctgataagta
     1201 gtcaagtcag attttttgac aatgagttta ttttttgcag tatagtaggg aatggaaaag
     1261 tcaaacacct tgctccgttc atctgtctta gaaacacctg atatggcaag gtcggctttt
     1321 cctgattgaa cactagccag tacattatca aaactcatgg gagatagttc caattcgaca
     1381 cctagttctg ttgcgatagc cttggctaat tcgatatctg aacccacaat ctgatttttc
     1441 ccatcaacca ctttttggta ttcaaatgga gcaaaatctg gattgagggc tacaaccaat
     1501 tttcctttgg acttgatggc ttcaattcca gccgattgat tgttgctaca agcaaatagt
     1561 agggggagca tcactaaacc aaacatcgtc atcaacacct tcttcatctt attcataaca
     1621 aagtctcctt tatgtttatt ctttttggtt gtataaataa tacacctatc ttcatccttt
     1681 gtcaagccta aattggaaaa attttcaatt tgaaacaaaa aaacctagaa atactacata
     1741 aaaatgtcat ttctaggcgt atttatgcta ttcctctctg aaaaacatga gtattcagtc
     1801 agtcaaaaag agctgaacaa actcatttcc cctcgcccaa ttcaatgatc cgatgacatt
     1861 gttgggctac ataagcatcg tgggtcacaa taatgactgt tttcccctcg cgattcatct
     1921 ctaagagaaa cttcaagacc aaatctctat tttcaggatc tagcgaacct gttggttcat
     1981 cggctaaaat cagctggctg ggttttaaga tggctctagc aactgcaatt cgttgttgtt
     2041 cgccaccaga caactcggaa accttttgat gcaaagtaga tgacaaacct actctctcta
     2101 aaatctcttc cacctttttg agcttgtctt tcttagacaa tttcacatat ttcagcgcca
     2161 gcatgagatt gtactcgacc gtttcatcat caatcagggc aaaattttga aacagataag
     2221 agatatgttc acggattatt gtttgcgact tagcagaatt gaccgctaga tttgtctgac
     2281 caaaaatctc ataccgtcca ctataatcac catctatcaa acctaataaa tttaacaagg
     2341 tcgacttccc actaccactt tttccaacaa tagctaccaa atccccctga tcaatcctga
     2401 gagacaagtg atccaaaatc acttttcccc caatggtttt ggtaatattt tccaactcaa
     2461 tcataagatg ccccctttca ataactctac tagacttctt ttctccatcc tagaagccaa
     2521 gactagcaca aatagtatat ccaaacatgt aaagcctgca aacagcagaa gtggcaagaa
     2581 cgcatgggca aagaaaatca agactagaag agggaaacta tagcccagca agagcagaac
     2641 gaggagagga cggtagcgat cgaccagttt ccaccccata aacttcttgg taatgatatc
     2701 cctgcgcttc aataagaaag ttattactag taagaagtag gaaatcatca tgctaaggag
     2761 accaaacaaa gcaaagagta ggttaaaatt ccgaacagca tctcgataag aatccacttt
     2821 ctcttgttga atggcttgaa tagatgaaaa ttttaaataa tttccatctg acaatttctc
     2881 aactaactct gtaatctctt tttgatgttg aaccgtattt tcaattttaa tcggattatt
     2941 taagccagtt gttgacaggg aggctttctc atcccacatc atatcagaat cattgaccaa
     3001 gctaataatt ggattggaga gattttcctt tcgcttatca ctatatggga aaaatgacca
     3061 atctccttca taataggcaa tctcgacatc catctcctct atcgttcgtt tttgctgctc
     3121 ttcatacttc atcgaatgaa aggcaattaa cttccccaag agctgatttt tatcttcttc
     3181 acctttcgta cttgctggca tcaaaataac ttttttaata ccggtatctg gtagcttgaa
     3241 tcccttgctc tttagaaaat tgcgattggc atagtaaaca tccaccgtat ctgttaactg
     3301 atattgctga atctgttctg attggacaaa attttttaca ggaagactgc tactctgcac
     3361 atagcccgcc tgcgtttttt ctaccaaatc ctgataaaat cgatagaaat aatccaatgc
     3421 cttccctgag cctgccaact gctcttgcca caagttatca ttgagttgga aagtttctaa
     3481 ggtcagataa tttccttggc tgatccactg tttctggtaa tccaactctt tgttttcttg
     3541 ctccaaactt tttcctaccc caactagcaa gactgtcagt aaaatggtag ttccaatttt
     3601 catcaaataa ttaaagagta gaccaaattt gaaagatgaa aaacctttca gcagagagct
     3661 gattgtcatt ttttggatta agaggtaagt caaccaactg ataaagagat aaagctgcaa
     3721 cagcaaaaaa tgagacaacc acagcatagg aaacaaatct tttggcttat aatcaagcaa
     3781 gaaaaacacg cctagattga tcacaagagc cccacctagg aggaggtaaa ggttgccttt
     3841 tacaacatca gctaaaacag ccctatcttg aaaaccaagt aatttttgta ccccaactct
     3901 tttcatctcc atcatcggtt gatacactgt cactaacaca agaagcaaaa tagccaagac
     3961 aaaaacaatg gcagataaaa gcaaatctcg atttatgact tccactgcac ttttgtaggt
     4021 cggctctagc aaggtagcct ggtctatctt gaaaaaatcg ctccatttct gtacaatcct
     4081 atccttgtcc atctcttgtg tagaagttat cgtatagcga ccatttaaac tacgagatgt
     4141 atccttgata taggtttgaa aagtcataag ctgaataggt ttggctttta gaaaggtcgg
     4201 aatcgtacca agtttattgg aaatttcttt attactatag actccttcac catctgtggt
     4261 aaaatcaaga gaagaaatcc caaactcttg gtaggggaag gtatctttat caaaaacacc
     4321 agacttgacc acctcatcac cactgtctgt tttgatgatg gagactttat actcctttga
     4381 tacatcctca aaaaatcgaa gaacagacgc tgcaggttcg ttaatatctt tcaaatacaa
     4441 atccaaagaa tctacagtct ttcccatttc ttgaatccga accacttctc gtgtatagtt
     4501 tacattaaaa acaaaaaaag tggtacacag aagcaggagt aacatacaaa gatgactgat
     4561 ttttttcata gagatacctc ccgtgaagaa actccctctc caaaagggga gggagaattt
     4621 taattaaaat ccatagaaat attccattcc cgttggtggg aatttgaaga ttgaagcccg
     4681 agcccatgcc tcagcttctg cacgatcctt agagaattta tttccatctt taacagttgc
     4741 tgtatgagag cgagtagaat gtaaataatc agaataacca aaatttcctg accatcctac
     4801 tccatagttc cattgaccac catcaaccca tactgccaaa acacttgtag aagcaagtat
     4861 actacatacc attaaagatg caacaccaat tttaactaat tttctcatga tttatttctc
     4921 ctattatcga gagctcttcc gtaaaaacag ctatagtaat ttatcaacaa gtatcgtagt
     4981 ccattggact caccttcctt ctatctcatt tcatttttta acaacgaact tactcttaac
     5041 cgagagaatg gtgcgcttct gtatcaggtc cttgaacaac cagagtagta ttaagcacac
     5101 tctaactatt actcaagaat cagtccaata aacaatatgg atagcgcttt caaattttta
     5161 aactcccatc tcatcctttt atctatataa taagtgaaaa tataataact gtcaagta
//
LOCUS       DAVBDG010000093         5159 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBDG010000093 DAVBDG010000000
VERSION     DAVBDG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.094"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(134..268)
                     /gene="rpmH"
                     /locus_tag="VQ526_001930"
     CDS             complement(134..268)
                     /gene="rpmH"
                     /locus_tag="VQ526_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2555608.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(413..1627)
                     /locus_tag="VQ526_001931"
     CDS             complement(413..1627)
                     /locus_tag="VQ526_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2555609.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLGIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1783..2076)
                     /locus_tag="VQ526_001932"
     CDS             complement(1783..2076)
                     /locus_tag="VQ526_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555610.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLIYPGFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            2238..2690
                     /locus_tag="VQ526_001933"
     CDS             2238..2690
                     /locus_tag="VQ526_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2555611.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIVHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2725..4113)
                     /locus_tag="VQ526_001934"
     CDS             complement(2725..4113)
                     /locus_tag="VQ526_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2555612.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            4179..5141
                     /locus_tag="VQ526_001935"
     CDS             4179..5141
                     /locus_tag="VQ526_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2555613.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
BASE COUNT         1500 a         1193 c         1009 g         1457 t
ORIGIN      
        1 cagaggtgta ctattctagt ttcaatctac tataacatcg cttccgaatc catttgcatc
       61 tactgaactt ttttacaaaa ataaaaatca gtagactcga gttcctactg atttttgata
      121 gaattcgttt ggattatgca gccaaaactt tgcgtccttt acgacgacga gctgccaata
      181 cgcgacgacc atttttagtt gacatacggt tacggaatcc gtgtttgcgc gcacgacgaa
      241 gtttacttgg ttgataagta cgtttcacga tgaatacctc ctcatagatt ttgtattcgt
      301 ttagccggct ataaagtgat cagttactaa acatacttta ctattctatc tgattctttc
      361 acttttgtca atagctctag gcaaatgttt ccagcttttt cgaatgcaag ccctatctac
      421 gatactgttt caagaaacga gtcatctttt cttggatttg ggctaactca tcaacacgag
      481 gaaggtaaac gatacggaag tggtctggtt cctgccagtt aaagcctcga ccatgaacca
      541 agagaacctt ttcctgcttc aagaaatcaa ggacaaactg ctcatcatca tcgatacggt
      601 acatattgcg gtcgattttt gggaagatat agagccccgc cttgggttta acggcagaca
      661 aacctggaat atcttgaatg gcattataga tgaaatttct ttgctcgtag attcgtccac
      721 caggaagaag caattcatcg actgattggt gaccccccaa ggaagtttgt acgacttgtt
      781 gggccaaaac gttagagcaa aggcgcatat tggacagcat attgagccct tcgatatagc
      841 ccttaacatg agtcttaggg ccagacaaga ccatccaacc cacacggaaa cctgctatgc
      901 ggtgggattt tgacagacca ttcatgctga cacagaagac atctggtgcc aagctcgcca
      961 caggcgtatg cacatgtccg tccattacca tgcggtcata gatttcatcc gcaaagataa
     1021 tcaaatcgtt ttgacgggca atctcaataa tccccagcaa gagttcctta ggataaaggg
     1081 ctccagttgg gttatttgga ttgataagga caattgcctt ggtattggaa gtgatttttg
     1141 acttgatatc gtcaatatct gggtaccatt ccacagcttc atcacagata tagtgaacgg
     1201 catttcctcc agctaggctg acagccgccg tccatagagg atagtctggc attggcacta
     1261 agacctcatc cccattgtcc aaaagcccct gcatggacat gacaatcaac tcactgacac
     1321 catttccaag gtaaatatca tcaatatcta cgtttgggaa tttcttcagc tggcaatact
     1381 gcatgatggc cttacgggct gagaaaatcc ctttggagtc agaatagcct tcactatcac
     1441 gcgcattcat aatcaagtca tgaatgacct cgtctggcgc tgtaaagcca aattctgctg
     1501 gatttcctgt attcagacgt aaaatctttt ctccattcgc tcgcatccgc atggcttctt
     1561 ccaatacagg accacggata tcataagcaa catgctctaa cttactagac ttgttatatt
     1621 ctttcatctt gtttcctcaa ttcatcagga tagtaacatt ataccactag aaagagaatg
     1681 cgacaagttt actgaaatgt gttactagtt tcgtatagaa gaaaaacctt gctaaaggcg
     1741 aggttaagaa cgtagagata gcaggtattt tgcaaagaca catcacatac caaaagataa
     1801 acctagaaaa agcaagccta gaaaggagtc ataaagaaga ttaaacatga ggaaaagccc
     1861 ccatatcatc aagtaatccc aacgcccact tcgttttgca actagaaata agattagatg
     1921 gtagagtaca tgaaagacta aaaatccaat aaaacctgga taaataagcg gtaccaaact
     1981 ctctaattgc cagatcatga tcacttctac caagactgaa actaggataa ttccataata
     2041 caggaaattt gatttttgaa aagagttttt catcattctc ccttccttca cttctttcct
     2101 gctatccttt cttttatttt atcatatatt tatatagatt ttaagcccta atattctttt
     2161 gaaaaatact ttttttgctt tacttctata gtgaaaaggt ttacaattat agtaagaaaa
     2221 tttcaggagg tttcattatg gcacaacgtt accaaaatat catggtcgca atcgatggtt
     2281 ctaaggaagc ggacttggct tttgtcaagg gagttcattc tgctctacga aacgacgcta
     2341 aactcaccat cgtacatgtc attgacacac gcgctctcca aagcgtatcc acctttgatg
     2401 ctgaagttta cgaagaactc caagtcgacg ctgaaagtct gatgaaagag tacgaaaaac
     2461 gtgctaaaga tgctggggta gcagatgttc atatcgtcat tgaaatggga aatccaaaga
     2521 ccctgctagc acgtactatt ccagatgccg aggaagtgga cctcatcctc gttggcgcaa
     2581 ctggtctcaa cgcctttgaa cgcctcttag tcggctcttc atctgaatac atactccgcc
     2641 atgctaaggt cgatttgctg gttgtgagag aacaagaaaa aaccttataa tcacaaagaa
     2701 aaggagcccc tagctccttt ttgtttacga tttatttctc tctttatggc gttcgtaagc
     2761 cttgagctgg cgctgcagtt cctttttaat agcaggttct ggagcatatt tttcttccca
     2821 attatctggt tttaagattt tatgggtcac tggatcaaaa tgagccttgc catctggaaa
     2881 aattttcccc atattggcct gatggacaat atcaaaaata cgttctgggt ccacccccat
     2941 caagacaaaa ctgccgtagg tgaagtaaag cgtgtcaatc aaggcatcca cttgccctat
     3001 caaatcttgc tgagcaggtg tcttcttggc tactttacct gctgccttat caagggcctg
     3061 atgaagttgc gatacagctt gaccaaaatc ttcttcagaa ggactggctg ctcgaacaaa
     3121 ctccaccaat tcttctattt taaaaccagc cctatgggtt gcaccctcta aatcccaagc
     3181 tcgaggttct tcttgggttc gttcatccat catgtggtgg aaggtcttga ccttattgaa
     3241 atgatagtca cggctgacaa agactttttc tgaagacaaa acaccaaaat gttccaaggc
     3301 cttgtggatc ccatcttgtt gattgctagt ggtaatgtgc ttggcagctt ccttgacact
     3361 gctactacca tttcccatag cgaccgacat accgacgcca gccagcattt caaggtcatt
     3421 atctgagtca ccaaaagcca tgacttggtt gaggtcaaag ccatattctt tcccaactcg
     3481 gcgaatgcct tctaatttag aatttccctg attgatgaca tccgatgcaa aaggattgct
     3541 acgtgtcaat ttcaagtctt caaaatcagc tgccgccttc tcagattctt ctggtgtcat
     3601 cagcatcaaa acttggtaga taggctgatt catcaggtga agcaggtcct cttccttttg
     3661 gggaacaacc ttgctgacca tgcgattaaa agactgactc accgtccgag ttaaaacaga
     3721 gggaacgaag cgactaattc gttgggaaaa agaacccaga ccaaaggaca tgattttaga
     3781 acccaacatg gcatccttgg tccctagagc aatctccgtg ccctcttttt tagcatagct
     3841 aattagatgg cgcaaatgta acttggaaat agggctcgtg aacaagactc tgtctttact
     3901 aaagatatac tggccattat aggttaccgc aaaatccaga tccaaatcgt ccatcaattc
     3961 cttaacaaaa aaaggtcctc gtcctgtcgc tacgccaact agtacccctt gttctttgac
     4021 aatcttaatc gcgtccttag tggatttcaa aacactcttg cgattgttga ccaaggttcc
     4081 atcgatatca aaaaaaacag ctttgacttc catcctattc caatctcccc ttttgtgata
     4141 caatgattat accacatttc agaaagagtt agtaaatcat gcctaagaaa atccttgttt
     4201 tacatacggg tggaactatt tccatgcagg ccgatgcttc tggcgctgtt gtgacgagtt
     4261 cagataatcc catgaaccat gtgtccaacc cacttgaagg aatccaagtc cacgccttgg
     4321 acttttttaa ccttccaagt ccccatatca aacccaaaca tatgctggtc ctctaccaga
     4381 aaattaaaga ggaagcagat aactacgatg gagtggtgat cacacacgga accgatactt
     4441 tagaggaaac agcctatttc cttgatacca tggaagttcc ccatatgcct atcgttctaa
     4501 caggagccat gcgtagctcc aatgagctcg gtagtgatgg tgtttataat tacctaagtg
     4561 ctttacgagt ggccagcgat gacagggctg ctgacaaagg agttttggtc gttatgaacg
     4621 atgaaatcca cgctgccaag tatgtcacca aaacacatac gactaatgtc agcaccttcc
     4681 agactccaac acatggcccc ctcggtctca tcatgaaaca ggaaatcctc tacttcaaaa
     4741 cagctgaacc tcgtgttcgc tttgaccttg atcacataca aggtttagtc cctatcatct
     4801 cggcttatgc tggtatgaca gatgagctga ttgatatgct ggatttagaa cacttggacg
     4861 gtttgattat ccaagccttc ggagctggta atattcccaa agaaacggct caaaaattag
     4921 aaagccttct gcaaaaagga atcccagtcg ctctggtatc acgatgcttt aacggtattg
     4981 ccgagcctgt ttatgcctac cagggtgggg gcgtacagtt gcaaaaagca ggcgttttct
     5041 ttgttaaaga actcaacgcc caaaaagctc gcttgaaact cctcatcgcc ctcaatgccg
     5101 gactaacagg acaggctttg aaagactata tggaaggcta atactcttcg aaaatctct
//
LOCUS       DAVBDG010000094         5135 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBDG010000094 DAVBDG010000000
VERSION     DAVBDG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.095"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(61..660)
                     /locus_tag="VQ526_001936"
     CDS             complement(61..660)
                     /locus_tag="VQ526_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555614.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTHPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(662..1759)
                     /locus_tag="VQ526_001937"
     CDS             complement(662..1759)
                     /locus_tag="VQ526_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2555615.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLVYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKASLTQDV
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTISDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(1759..2496)
                     /locus_tag="VQ526_001938"
     CDS             complement(1759..2496)
                     /locus_tag="VQ526_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555616.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(2498..3382)
                     /locus_tag="VQ526_001939"
     CDS             complement(2498..3382)
                     /locus_tag="VQ526_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555617.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPNIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(3369..3563)
                     /locus_tag="VQ526_001940"
     CDS             complement(3369..3563)
                     /locus_tag="VQ526_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555618.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLCQFIRGALEFGFM
                     IFVVFPMGMEWLKGESHDGG"
     gene            complement(3560..3766)
                     /locus_tag="VQ526_001941"
     CDS             complement(3560..3766)
                     /locus_tag="VQ526_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555619.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
     gene            complement(4355..4428)
                     /locus_tag="VQ526_001942"
     tRNA            complement(4355..4428)
                     /locus_tag="VQ526_001942"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4392..4394),aa:Pro,seq:tgg)
     gene            complement(4444..4517)
                     /locus_tag="VQ526_001943"
     tRNA            complement(4444..4517)
                     /locus_tag="VQ526_001943"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4481..4483),aa:Arg,seq:acg)
     gene            complement(4527..4612)
                     /locus_tag="VQ526_001944"
     tRNA            complement(4527..4612)
                     /locus_tag="VQ526_001944"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4576..4578),aa:Leu,seq:taa)
     gene            complement(4620..4691)
                     /locus_tag="VQ526_001945"
     tRNA            complement(4620..4691)
                     /locus_tag="VQ526_001945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4657..4659),aa:Gly,seq:gcc)
     gene            complement(4714..4786)
                     /locus_tag="VQ526_001946"
     tRNA            complement(4714..4786)
                     /locus_tag="VQ526_001946"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4751..4753),aa:Thr,seq:tgt)
     gene            complement(4805..4886)
                     /locus_tag="VQ526_001947"
     tRNA            complement(4805..4886)
                     /locus_tag="VQ526_001947"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4850..4852),aa:Leu,seq:tag)
     gene            complement(4892..4964)
                     /locus_tag="VQ526_001948"
     tRNA            complement(4892..4964)
                     /locus_tag="VQ526_001948"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4929..4931),aa:Lys,seq:ttt)
     gene            complement(4989..5061)
                     /locus_tag="VQ526_001949"
     tRNA            complement(4989..5061)
                     /locus_tag="VQ526_001949"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5026..5028),aa:Asp,seq:gtc)
     gene            complement(5064..5135)
                     /locus_tag="VQ526_001950"
     tRNA            complement(5064..5135)
                     /locus_tag="VQ526_001950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5101..5103),aa:Val,seq:tac)
BASE COUNT         1626 a         1188 c          877 g         1444 t
ORIGIN      
        1 gtattgttcc attcactacc gtcaatttta gcacatagtc ttcatgaaaa tattatatca
       61 tcataaccaa ccagattctt ttgcgatatt agctgcctct gttcgattac cagcatccag
      121 ttttgaaaga atattggtga catagtttcg aatagttccg tttgagaggt aaagctgatc
      181 tgcgatttct tggttggata agccccgggc gattcccttt aaaacagcaa tctcttgttc
      241 tgtcaacgga ttaggatgcg tcatcaccac ttccatcaat tcaggcgaat actccttgcg
      301 cccttcgagg acggtgtgca aggtttgcat gaggtctgca atgcttcttt ctttcaagac
      361 ataagcatcc actccagcct tgaccgcacg ttcaaaatac cctggacgct tgaaggtcgt
      421 caccacaacc acctttgttt caagcctttc tgatcgtatc cactccaaga cttcaagacc
      481 tgtcttaaca ggcatttcta cgtcaaggat ggcgatatct acagactcct tttctaatag
      541 ttggattgct tcttgcccat tcttggcttg aaggacagac tccacatctg gttgaagcgt
      601 gagcaattga cacatggcat ctcgcaacat actttgatct tctgcgacta atactttcat
      661 cttagtttct ctccttataa ggtagtcgaa cttgcacttc cgttggatgt ttctgactga
      721 ttacacttac ttctcctgaa aatggaaaaa cacgatctcg gactgtatgg agctcatctc
      781 cttttagaaa agcaaagccg cagccatcat cacttattgt taaaatgagt tccttctctg
      841 tccgttctaa ttttaagtaa gctttagacg ctttggcatg tttgatgata ttggtcacca
      901 actcaagcaa aatcatagaa gccattgact ccacatcctg ggtcaagcta gccttgtcta
      961 agtgattctc aacttgaacc tcaattccag caatttctaa catctttttc acagtctcta
     1021 gttcggatgt caaagttcta gacttaagat tttccacaat ggttcgcact tcattcatgg
     1081 aatccttgct gatctggtga atttctttta attccttttc cacctgtgga taagcctcca
     1141 tctgaaataa ctgcaaggct agatcagttt taacactaag catagcaaag gtatgcccca
     1201 gactatcatg caaatcttga ccgatacgat tgcgttcatt ttcagcaagc aatagattta
     1261 tctgggcatt ttgcttaacc tgtgcttctt tcaaatcctc cacaattcga atccgaacca
     1321 agccaaaagt cattaaatcg acaaaagcaa gaattacaag tagatagact agaaactcaa
     1381 cttcgattct ctgaaaaatc aacagttgac ccacaacaag gacttgagca agaagaaaag
     1441 tccagacatg taaagacttt aaaccaccta cgctgaaatg atagcttaag agattggata
     1501 ggaaaaagaa aaaccagata taattaacag ccacaaaggc agtattccca actacataag
     1561 tcagcaggat gccccaatat agccaagata gacgctggct cttagttgtt aaaacaccca
     1621 aatacgccac tacaaataga atatcaatca ataaatgcca ggcagaaagc cacccagtca
     1681 ctacaggtag gatggggaaa accataaaaa ttaaactgat ccaaaacata taatgtatgc
     1741 ttttcagtct ttcaagcatt aagcattctc cttatgacct tgaaggtaaa tggtcaaacc
     1801 aaacaaaact actgaaaaaa caagtaaata aactgtggct gatagattga tgccaccctc
     1861 atttaagaag gtcttgagca actccatcaa ctgataggtc gggagacact tacctactac
     1921 ttgcatccag tctggaaata aagaaatggg catccagagt ccgcctaaaa cagccaatcc
     1981 tagataaaga agattgccca caacagacat cagctgacta gttggtaaga gagtcaaggt
     2041 caagccaagt gctacaaaag caatactccc cacaatcagc aaaatcgcag ctccaaccca
     2101 ttttactaga ggcatgtcta cacctcttac aaaatgtcca actgtaaaaa caaccagaat
     2161 tgagaccaaa taatcaacca gcatacttgt tatctttgat agataatatt ctaccatatt
     2221 tacagggcta tgacgcaatg ttttctgcca gttgttcatc ttatcggtat gtaaaacagc
     2281 tggaaatgag aggatagttg tcgacatcat ggaaaaggca gtcatggaga tgaggtaatc
     2341 acgcataaaa ttcgcgagtt cacctggtgt gtcctgatag ataccagaaa aaaataaata
     2401 gaaagccgtc ggcatcccta ccgacaatag ataatagatt aattgtcgtt tggtcaataa
     2461 aaattctatc ttactaagtg ctatccatcg tttcatctta gttatctccc ttctgcgtct
     2521 cttcaaagat tgtattcaac aaactacgat tattaacttc gatttcttgc atcctacatc
     2581 ctgcttgggc taatagttcc cagaaagcat ctgcttctcg agtgactact tgtaaagaat
     2641 cttgctttag ggtccagttt tcaaccaaat ttgactgctc tacgacttcc ttgtaagcta
     2701 taggaaggat aaagtgcttt tctatttcct cgctacgcat agctagaggc gtcgtatctc
     2761 gaatcaactc tcccttattt aagagcaaaa tgcggtctgc agtgtgttcc acttcctcaa
     2821 tataatggga cgaatagaga atggtgactc cttgcgcttt taactcctgg acaatttccc
     2881 aaaaacgttg acgagttgag gtatccatgg acgcagtagg ctcatctaaa aaaacaatat
     2941 ttggtcgccc aattaaggtc aagacgaaag aaaagagacg cttttgaccg cctgataatt
     3001 tttctgccaa ctgttctttt tgttgcttga caaactgcaa tagttgatca atttcctgat
     3061 tgctcaaagg atttggatag atacgttgaa agaaagcaat caactcttta acttttaatt
     3121 tctgaacgat tacattttct tgagggaggt aagccctagt atagtctaac ttagaactcg
     3181 tcactggtaa accttgaata gatacttgac cgcttgtgac cagtttatct ccaagcaaac
     3241 aatctaagag tgttgtcttt ccggcaccat ttggaccaat taaggcaaca cattcaccat
     3301 cgttgatttc aaaggagatg ttccgcaaaa tttccttgtc ttttattttc ttactcaatt
     3361 tctcaacttt aaccaccgtc atgagactct cctttcaacc actccattcc catagggaaa
     3421 acgacgaaaa tcataaatcc aaactccaaa gcaccacgaa tgaattggca aagcaaggct
     3481 tgatcaaacc aatcagtaaa catttccact aaccatacca agagtgacag gccgataaag
     3541 aaatagatga tccctctctt cattcttcaa actccttttt cacatctctg actaatttca
     3601 aaccttctct aacaagccaa gacatgattc caaagccagc aaagaccttc caaggaaaat
     3661 ggtagaaatc ctcatccaat cccgaaaaca tgagataggt cataactcct gctactacta
     3721 aactcattgc aataatcatt ttatttctca tctcttcttc ccccatttca tactacaatt
     3781 atagtctttt aaaatcagag gaggcagaag cttctgtcac tagaaaatat gacaaatgtc
     3841 ataaaaaatt ctgttcaaaa caagcaagat acattataca ataaaacaca atcagaaaaa
     3901 tctaaggcaa cttcctcaaa agagatatca aactcaattc acaccataat gtaaactaat
     3961 acttatttaa aatcaaaaag agtagaaatt tttatcagac aaacacatat atagtgtatt
     4021 gaatctataa cagtaggcct taaatactaa aatatttcta taaattaatt taactttcct
     4081 gatagagctg ttcatatctt atttcaattc tctatattat acgttgaaca aaacccttct
     4141 attgctttct taaaaattta taagagttat aaaatctgtt aaatttcaat gtgtatacct
     4201 aaactacggt atttattgaa aagactggag acaaaaagta tacgctgcca aaatgaatta
     4261 ctgaaaatca aaaaagagag aaccaaactg attccctctt aatgtatata atatctagtt
     4321 ttaaaaatac acactcacat atctctgtaa tgaatcggga agacaggatt cgaacctgcg
     4381 acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag ttaaatagaa
     4441 aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg tactctatcc
     4501 agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg tacgatcgtt
     4561 accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg gcctctctaa
     4621 gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt ctaccactga
     4681 actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt gaacacgcga
     4741 ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat ttgttatatc
     4801 ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc taaatctggt
     4861 gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga accagtgacc
     4921 cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat aacttgcgtt
     4981 atcttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag ggcgacgtga
     5041 taaccgctac actacgggac ctttgggagt taacgggatc gaaccgctga ccctctgctt
     5101 gtaaggcaga tgctctccca gctgagctaa actcc
//
LOCUS       DAVBDG010000095         4996 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBDG010000095 DAVBDG010000000
VERSION     DAVBDG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.096"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            298..1843
                     /locus_tag="VQ526_001951"
     rRNA            298..1843
                     /locus_tag="VQ526_001951"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1895..1967
                     /locus_tag="VQ526_001952"
     tRNA            1895..1967
                     /locus_tag="VQ526_001952"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1928..1930,aa:Ala,seq:tgc)
     gene            2092..4993
                     /locus_tag="VQ526_001953"
     rRNA            2092..4993
                     /locus_tag="VQ526_001953"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1408 a         1017 c         1468 g         1103 t
ORIGIN      
        1 cgccttttca cggcggtaac acgggttcga atcccgtacg gactatggta tgttgcggtt
       61 ggaacacttg atgaaaaaaa gtttaaaaaa gcttaaaaat cttcaaaaaa gtgttgacaa
      121 gcgaaagcgg ctgtgatata ctaatatagt tgtcgcttga gagaagcgag tgacaaagac
      181 ctttgaaaac tgaacaagac gaaccaatgt gcagggcgct acaacataag ttgtagtact
      241 gaacaatgaa aaaaacaata aatctgtcag tgacagaaat gagtgagaac tcaaactttt
      301 aatgagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagta
      361 gaacgctgaa ggaggagctt gcttctctgg atgagttgcg aacgggtgag taacgcgtag
      421 gtaacctgcc tggtagcggg ggataactat tggaaacgat agctaatacc gcataagagt
      481 agatgttgca tgacatttgc ttaaaaggtg cacttgcatc actaccagat ggacctgcgt
      541 tgtattagct agttggtggg gtaacggctc accaaggcga cgatacatag ccgacctgag
      601 agggtgatcg gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagta
      661 gggaatcttc ggcaatggac ggaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt
      721 ttcggatcgt aaagctctgt tgtaagagaa gaacgagtgt gagagtggaa agttcacact
      781 gtgacggtat cttaccagaa agggacggct aactacgtgc cagcagccgc ggtaatacgt
      841 aggtcccgag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg ttagataagt
      901 ctgaagttaa aggctgtggc ttaaccatag taggctttgg aaactgttta acttgagtgc
      961 aagaggggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac
     1021 cggtggcgaa agcggctctc tggcttgtaa ctgacgctga ggctcgaaag cgtggggagc
     1081 aaacaggatt agataccctg gtagtccacg ctgtaaacga tgagtgctag gtgttagacc
     1141 ctttccgggg tttagtgccg tagctaacgc attaagcact ccgcctgggg agtacgaccg
     1201 caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta
     1261 attcgaagca acgcgaagaa ccttaccagg tcttgacatc cctctgacga ctctagagat
     1321 agagttttcc ttcgggacag aggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt
     1381 gagatgttgg gttaagtccc gcaacgagcg caacccctat tgttagttgc catcatttag
     1441 ttgggcactc tagcgagact gccggtaata aaccggagga aggtggggat gacgtcaaat
     1501 catcatgccc cttatgacct gggctacaca cgtgctacaa tggctggtac aacgagtcgc
     1561 aagccggtga cggcaagcta atctcttaaa gccagtctca gttcggattg taggctgcaa
     1621 ctcgcctaca tgaagtcgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg
     1681 ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg
     1741 tgaggtaacc gtaaggagcc agccgcctaa ggtgggatag atgattgggg tgaagtcgta
     1801 acaaggtagc cgtatcggaa ggtgcggctg gatcacctcc tttctaagga taaggaactg
     1861 cgcattggtc ttgtttagtc ttgagaggtc ttgtggggcc ttagctcagc tgggagagcg
     1921 cctgctttgc acgcaggagg tcagcggttc gatcccgcta ggctccattg gtgagagatc
     1981 accaagtaat gcacattgaa aattgaatat ctatatcaaa tagtaacaag aaaataaacc
     2041 gaaaacgctg tagtattaat aagagtttat gactgaaagg tcagaaaata aggttaagtt
     2101 aataagggcg cacggtggat gccttggcac taggagccga cgaaggacgt gacaaacgac
     2161 gatatgcctt gggtagctgt aagtaagcga tgatccatgg atttccgaat gggggaaccc
     2221 aacaggtaat acctgttacc cacatctgtt aaggatgtga ggaggaagac gcagtgaact
     2281 gaaacatcta agtagctgca ggaagagaaa gcaaaagcga ttgccttagt agcggcgagc
     2341 gaaacggcag aagggcaaac cgaagagttt actcttcggg gttgtaggac tgcaatgtgg
     2401 actcaaagat tatagaagaa tgatttggga agatcagcca aagagagtaa tagcctcgta
     2461 tttaaaatag tctttgtacc tagcagtatc ctgagtacgg cgggacacgt gaaatcccgt
     2521 cggaatctgg gaggaccatc tcccaaccct aaatactccc tagtgaccga tagtgaacca
     2581 gtaccgtgag ggaaaggtga aaagcacccc gggaggggag tgaaatagaa cctgaaaccg
     2641 tgtgcctaca acaagttcga gcccgttaat gggtgagagc gtgccttttg tagaatgaac
     2701 cggcgagtta cgttatgatg cgaggttaag ttgaagagac ggagccgtag ggaaaccgag
     2761 tctgaatagg gcgccttagt atcatgacgt agacccgaaa ccatgtgacc tacccatgag
     2821 caggttgaag gtgcggtaag acgcactgga ggaccgaacc agggcacgtt gaaaagtgct
     2881 tggatgactt gtgggtagcg gagaaattcc aaacgaactt ggagatagct ggttctctcc
     2941 gaaatagctt tagggctagc gtcgacatta gagattcttg gaggtagagc actgtttggg
     3001 tgaggggtcc atcccggatt accaatctca gataaactcc gaatgccaat gaattatggt
     3061 cggcagtcag actgcgagtg ctaagatccg tagtcgaaag ggaaacagcc cagaccacca
     3121 gctaaggtcc caaaataatt gttaagtgga aaaggatgtg gggttgcaca gacaactagg
     3181 atgttagctt agaagcagct attcattcaa agagtgcgta atagctcact agtcgagtga
     3241 ccctgcgccg aaaatgtacc ggggctaaaa caatttaccg aagctgtgga tacctttata
     3301 ggtatggtag gagagcgttc tatgtgtgat gaaggtatac cgtgaggagt gctggaacgc
     3361 atagaagtga gaatgccggt atgagtagcg aaagacaggt gagaatcctg tccaccgtaa
     3421 gactaaggtt tccaggggaa ggctcgtccg ccctgggtta gtcgggacct aaggagagac
     3481 cgaaaggtgt atccgatgga caacaggttg atattcctgt actagagtat gtagtgatgg
     3541 agggacgcag taggctaact aaagcagacg attggaagag tctgtctaag cagtgaggtg
     3601 tgaattgagt caaatgctta attctataac attgagctgt gatggggagc gaagtttagt
     3661 agcgaagtta gtgacgtcac actgccaaga aaagcttcta gcgtttaaac atactctacc
     3721 cgtaccgcaa accgacacag gtagtcgagg cgagtagcct caggtgagcg agagaactct
     3781 cgttaaggaa ctcggcaaaa tgaccccgta acttcgggag aaggggtgct gactttaagt
     3841 cagccgcagt gaataggccc aagcaactgt ttatcaaaaa cacagctctc tgctaaatcg
     3901 taagatgatg tatagggggt gacgcctgcc cggtgctgga aggttaagag gagtgcttag
     3961 cgtaagcgaa ggtatgaatt gaagccccag taaacggcgg ccgtaactat aacggtccta
     4021 aggtagcgaa attccttgtc gggtaagttc cgacccgcac gaaaggcgta atgatttggg
     4081 cactgtctca acgagagact cggtgaaatt ttagtacctg tgaagatgca ggttacccgc
     4141 gacaggacgg aaagacccca tggagcttta ctgcagtttg atattgagtg tctgtaccac
     4201 atgtacagga taggtaggag tctaagagat cgggacgcca gtttcgaagg agacgctgtt
     4261 gggatactac ccttgtgtta tggccactct aacccagata ggtgatccct atcggagaca
     4321 gtgtctgacg ggcagtttga ctggggcggt cgcctcctaa aaggtaacgg aggcgcccaa
     4381 aggttccctc agaatggttg gaaatcattc gcagagtgta aaggtataag ggagcttgac
     4441 tgcgagagct acaactcgag cagggacgaa agtcgggctt agtgatccgg tggttccgta
     4501 tggaagggcc atcgctcaac ggataaaagc taccctgggg ataacaggct tatctccccc
     4561 aagagttcac atcgacgggg aggtttggca cctcgatgtc ggctcgtcgc atcctggggc
     4621 tgtagtcggt cccaagggtt gggctgttcg cccattaaag cggcacgcga gctgggttca
     4681 gaacgtcgtg agacagttcg gtccctatcc gtcgcgggcg taggaaattt gagaggatct
     4741 gctcctagta cgagaggacc agagtggact taccgctggt gtaccagttg tcttgccaaa
     4801 ggcatcgctg ggtagctatg tagggaaggg ataaacgctg aaagcatcta agtgtgaaac
     4861 ccacctcaag atgagatttc ccatgattat atatcagtaa gagccctgag agatgatcag
     4921 gtagataggt tagaagtgga agtgtggcga cacatgtagc ggactaatac taatagctcg
     4981 aggacttatc caaagt
//
LOCUS       DAVBDG010000096         4926 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBDG010000096 DAVBDG010000000
VERSION     DAVBDG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.097"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            47..408
                     /locus_tag="VQ526_001954"
                     /pseudo
     CDS             47..408
                     /locus_tag="VQ526_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
     gene            1453..2445
                     /locus_tag="VQ526_001955"
     CDS             1453..2445
                     /locus_tag="VQ526_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2555620.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQNNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINKEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2447..3265
                     /locus_tag="VQ526_001956"
     CDS             2447..3265
                     /locus_tag="VQ526_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2555621.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3267..4052
                     /locus_tag="VQ526_001957"
     CDS             3267..4052
                     /locus_tag="VQ526_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555622.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4091..4369
                     /locus_tag="VQ526_001958"
     CDS             4091..4369
                     /locus_tag="VQ526_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2555623.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4354..4551
                     /locus_tag="VQ526_001959"
     CDS             4354..4551
                     /locus_tag="VQ526_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555624.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1415 a          810 c         1033 g         1668 t
ORIGIN      
        1 ggctagtttc ctagtttgct ctttggtttt cattgagtat aacacattgt tagaagttgg
       61 tttaaatttc ctaatcagtt tgttcacatt taccttcgat atattatatc ccatagttaa
      121 ggttggtcat acagatgatt atagtcatgg agccgtaaaa cttagtgttt ctttagttga
      181 caaagatgcc atgaaaaaat atctgtaact gtaataggat attttgaaat aaatatagat
      241 gaaaatatca ccgatattct atacgtaaat ggtactgcta ttctttgtcc ttatttacgt
      301 tctattgttt caatagtttc ggcaattgat agcagtgaag caatgttgct acctaccatt
      361 aatgttttag agttactaga taaatctcaa ccttttgaag aagaataatt tattagctca
      421 ctaaattgag ggtaaggaaa agtaaaagca gtaagaaaaa tgtcttgcat tatacagcaa
      481 ccttttggga atgagtagat ggattgaata aaatttgatt aagagtggat gatttatctg
      541 tagattatta ttggacagtt agtcttgaag tagtctaaga attaggttat aatcagtaga
      601 agccttgcta ataatgagga ggttagttta tgtatagtag actgaatcta aaatagtacg
      661 aaacaattgc taaaacattt atagaaatta attttacttt cccaatcgat ttgttctcat
      721 cttatttcaa tccgctatat attatggtat cgaatcttca tcagagtgat aaaattaatc
      781 aattaatatc tgattacaaa cagaatatga aagcttttta tatcactatt gaaaaattta
      841 tacgagatga tgaaagcctt aagtgttatt ttataaaggt tatttcaagt cgttccaagg
      901 taacaagtct agatcagatt gaagctgata aaacgataca aagaaaatat tcaagtgagc
      961 taaaaaaatt tattggattt tataatgaga ttatttgtga ggaaaatagt ttcctacatg
     1021 tacgaaagag gtggtcgagt tggtttaggt agtcggtgcg tgagttgata attctcaggg
     1081 tatggacttc tttttcatga atgaggtaaa agagcaggta ttgtttagag acaatcattc
     1141 tgagcatatt ttctggatag agggagtatc cgattttatg atcaaagtta ataccgccct
     1201 ctggtgagaa gatgagtagg ttggtaattt aaactattaa acagaatttt tgattaaaag
     1261 tattatttta tgagagaaat cctaatttta caatccatag gcaaacgctt gcatttcgtt
     1321 ttttattgga ctataatagg ttggtataaa gccttttgta gtaataaaat gtagaaggtg
     1381 tagaaagtaa ggatttagaa tatttgtagt taaaaacaca atgttgctat tccttacgat
     1441 agggagatag atatggcaat gatagaagtg gaacatcttc agaaaaattt tgtgaagact
     1501 gttaaggaac cgggcttgaa gggggctttg cgctccttta ttcatcctga aaagcagacc
     1561 tttgaagcgg tcaaggattt gacctttgag gttccaaaag ggcagatttt aggatttatc
     1621 ggggcaaatg gtgctgggaa gtcgacaacc attaaaatgc tgacaggaat tttgaaacca
     1681 acatctggtt tttgtcggat taacggcaag attccccaga acaatcggca agattatgtc
     1741 aaagatattg gcgtagtctt tggacaacgc acccagctat ggtgggattt ggctctgcaa
     1801 gagacctaca ctgtcttaaa agagatttat gatgtgccaa actcgctctt tcataagcgc
     1861 atggactttt tgaatgaagt cttggatttg aaggacttta tcaaggatcc cgtgcggact
     1921 ctttcactgg gacaacggat gcgggcggat attgcggcct ccttgctcca caatcccaag
     1981 gttctttttt tagatgagcc gaccattggt ttggacgttt cggttaagga taatattcgt
     2041 cgggcaatta ctcagatcaa taaagaggaa gaaactacca ttcttttgac cactcacgat
     2101 ttgagtgata ttgagcaact ttgtgatcgg attttcatga ttgacaaggg gcaagagatt
     2161 tttgatggaa cggtgagcca actcaaggag acctttggta agatgaagac tctctctttt
     2221 gaactgctac caggtcaaag tcatctcgtc tctcactatg aaggtctgtc tgatatgacc
     2281 attgatagac aaggaaacag cctcaacatt gaatttgata gttctcgcta ccagtcagct
     2341 gacattatca agcaaaccct atctgatttt gaaatccgcg atttgaagat ggtggatacg
     2401 gatattgagg atattatccg tcgcttctac cgaaaggagc tctaggatga tcaaattgtg
     2461 gagacgttat aaacccttta tcaatgcagg ggttcaggag ttgattactt accgagtcaa
     2521 ctttattctc tatcggattg gcgatgtcat gggggctttt gtggcctttt atctctggaa
     2581 ggctgtcttt gattcttcgc aagagtcttt gattcagggc ttcagtatgg cggatatcac
     2641 cctctacatc atcatgagtt ttgtgaccaa tcttctgact agatccgatt cgtcctttat
     2701 gattggggag gaggtcaagg atggctccat tatcatgcgt ttgttgcgac cagtgcattt
     2761 tgcggcctcc tatcttttca ccgagcttgg ttccaagtgg ttgattttta tcagcgttgg
     2821 ccttccattt ttaagtgtca ttgtcttgat gaaaatcata tcgggtcaag gtattgtaga
     2881 ggtgctagga ttaactgtcc tttatctttt tagcttaacg ctcgcctatc tgattaactt
     2941 tttctttaat atttgctttg gattttcagc ctttgtgttt aaaaatcttt ggggttccaa
     3001 cctacttaag acttccatag tggcttttat gtcggggagt ttgattccct tggcattctt
     3061 tccaaaggtt gtttcagata ttctctcctt tttgcctttt tcatccttga tttatactcc
     3121 agttatgatc attgttggaa aatacgatgc cagtcagatt cttcaggcac tccttttgca
     3181 gttcttctgg ctcttagtga tggtgggatt gtctcagttg atttggaaac gggtccagtc
     3241 ctttatcacc attcaaggag gttagtatga aaaaatatca acgaatgcat ctgattttta
     3301 tcagacaata catcaaacaa atcatggaat ataaggtgga ttttgtggtt ggtgtcttgg
     3361 gagtctttct ggctcaaggc ttgaatctct tgtttctcaa tgtcatcttt caacatattc
     3421 cattcctaga aggctggacc tttcaagaga tagctttcat ttatggattt tccttgattc
     3481 ccaagggaat ggaccatctc ttttttgaca atctctgggc actagggcaa cgcctagtcc
     3541 gaaaaggaga gtttgacaag tatctgactc gtcccatcaa tcctctcttt cacatcctag
     3601 ttgaaacctt tcagattgat gccttgggtg aactcttagt cggtggtatt ttattgggaa
     3661 caacagtgac cagcattgtt tggactcttc caaaattcct gcttttccta gtttgtattc
     3721 cttttgcgac cttgatttat acttctctta aaatcgcaac agccagtatc gccttttgga
     3781 ctaagcagtc aggcgccatg atttacatct tctatatgtt caatgacttt gctaagtatc
     3841 cgatttctat ttacaattct cttcttcgtt ggttgattag ctttatcgtg cctttcgcct
     3901 ttacagccta ctatccagct agctatttct tacaggaaaa ggatgtgttc tttaacgtag
     3961 gaggtttgat gttgatttct ctggttttct ttgttatttc ccttaaactt tgggataagg
     4021 gcttagattc ctacgaaagt gcgggttcgt aaaagctaaa gtaagactaa aatcaagaaa
     4081 gaaacttatg atgtttgtaa ttgaagaagt caaggatgaa aatcaaaaaa aggcagttgt
     4141 agctgaggtt ttgaaggatt tgccagaatg gtttggaatc ccagaaagca cacaagccta
     4201 tatagaagga accacgacac tgcaagtttg gaccgcctat caggagagtg atttgactgg
     4261 atttgtaagc ttatcctatt cgagtgaaga ttgtgcagag attgattgtc tcggcgtaaa
     4321 aaagcttatc aaggtagaaa aattgggagc caattgcttg ctactttaga gagtgaagct
     4381 cgtaaaaaag ttggttatct gcaggtcaaa acagtagcag aaggttctaa taaagattat
     4441 gatcgaacaa atgactttta tcgaggtctt ggctttaaaa agttagagat ttttcctcaa
     4501 ctatggaatc cgcaaaatcc ttgtcagatt ttgattaaaa agcttgaata atattatttg
     4561 acatctattc tcagagtgct atactgtaag tgtaatcgcc gatttagctc agttggtaga
     4621 gcaaggcact cgtaaagcct aggttatagg tagataaacg actgaggatt tgaaaaaata
     4681 gataggtaga agataaccgt taagccttat tcttagcggt tatttatatt gtttaatagc
     4741 gctgatattt catcaattat gcctgttttc gtgtttctgg tagttgttca agtttattgc
     4801 tactattttt gatggtatga atgtgcttat aatgtatccc ggttaacgaa agttttggac
     4861 ttatactctt agaaaatctc ttcaaaccac gtcaacgtcg ccttgccgtg cgtatggtta
     4921 ctgact
//
LOCUS       DAVBDG010000097         4725 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBDG010000097 DAVBDG010000000
VERSION     DAVBDG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.099"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..217)
                     /locus_tag="VQ526_001960"
     CDS             complement(<1..217)
                     /locus_tag="VQ526_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2555625.1"
                     /translation="MNDEASKQLTDARFKRLVDVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIA"
     gene            complement(597..1457)
                     /locus_tag="VQ526_001961"
     CDS             complement(597..1457)
                     /locus_tag="VQ526_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2555626.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(1473..2750)
                     /locus_tag="VQ526_001962"
     CDS             complement(1473..2750)
                     /locus_tag="VQ526_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2555627.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(2743..3294)
                     /locus_tag="VQ526_001963"
     CDS             complement(2743..3294)
                     /locus_tag="VQ526_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555628.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(3310..4569)
                     /locus_tag="VQ526_001964"
     CDS             complement(3310..4569)
                     /locus_tag="VQ526_001964"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2555629.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
BASE COUNT         1460 a         1080 c          758 g         1427 t
ORIGIN      
        1 ccgcaatttg ttcataagtt cgatattctc gcacatattg aagagtggcc ataagaaggt
       61 cttctaggct taatttaggt tttcttccac cttttgcatg tttaagttga taagctgttt
      121 ttaatacagc taacatctct tcaaaagtgg tacgctgaac atcaacaaga cgcttaaatc
      181 gtgcatcagt tagttgttta cttgcttcat cattcataga actactatac catgttttgt
      241 ttcgcaggaa gtataatata aaaaaataaa tttaagattg gcaatacgaa cgttaaacag
      301 aattccattt ctctttttag aatcaaaacg acaatattgt ggaatctcta caggaacaat
      361 agactgcgac actaaaatat cctcccttag ttttacttta ctaatagtat ataggaggag
      421 agccctgaat cctagaccct ttgttatgat gcgcttacaa acagagttgg aaaaaatctt
      481 aaaagcagaa aaacgcatga tatcagcccc taacacttga tacaatgcgt tttattatga
      541 aaagatctac tggatttttt atcaaaataa acttccagta gtcttttcac tttttattaa
      601 taagttcctt cttcaccttg gctagtcaag ataacaggtc catctttcgt aatgacaaat
      661 tggtgttcat actgacatga caatccaccg tcaatggtct tatgcgccca accagttttc
      721 atatctgtat caatttccca atcgcctgta ttgatcattg gttcaatggt taagaccatt
      781 ccttcacgaa gacggagtcc acgacctgca ataccatagt taggaaccat tggttcttcg
      841 tgcatagttg ggccaacacc atgaccaacc aaatcacgca ctacaccgta accacgactt
      901 tcagcgtatt cttgaatagc cgcaccgata tcaccgatac gatttccaac aacagcttgc
      961 tcaataccct tgtacatagc ttctttggtt acatccatca agtttttgac ttcttcggac
     1021 ggtgtaccaa cagcataagc ccaacatgag tctgctaaac caccagaata gctctgagta
     1081 tattttttca tttgttcaac attgttgaag tttaattttg agacattcag gtcagattta
     1141 gcaatgggac ctcccaaaac catatcaact ttgagcaaat caccatcttt caagatataa
     1201 tgacgaggga aagcgtgagc cacttcatcg ttaagagagc aacaggtagc ataaggatag
     1261 tccatcatgg caccgtcaac cccaatctga agtggaagga aattttcttc tttacaacga
     1321 cggcggacat attcttcaac ttcccacata tctacgcctg gcttaatcaa atcacgtaag
     1381 cctatatgaa tacttgctag aaaatcacca gccttgtcca tagcttcgat ttcacgagct
     1441 gattttaatg ttatcatctt ttctcctagt ttctaattaa ttttaacagt cacatttgct
     1501 tttgaaacaa tctggtgacc atgataaata tcgtaatcaa ttatagctga ccgtctcgta
     1561 tgatgaataa tccgtgcctg aatgcgcaat atatcatcta tctgaacagc ctgcaaaaag
     1621 tagatcagca tctgctcgat aatgagattg cgaccactat taacaactaa atcttgggtc
     1681 atgtgggtca gaatttctgc caatacacca ttagccaaaa ctccattttt ttctagcata
     1741 aagggttcca ctgtaatgac tacttcatca tggtgataag agagcttttg tccaatctgc
     1801 tcagaaaaag ttggtagagc cgaaacttgg gaacggctca tcttctccat gacatctcgt
     1861 cgcgtcacaa cgccaagcaa agtttgattg cttcgaacaa ctggtaccat ttcaaagtct
     1921 tctgcgatca tccgttgact cacattggca atatttgtcg ataatccaac caaaaataga
     1981 ctacgagaca taaccttatc aattgtcgtg cttggtgatt tatcaccagc gtctctcatg
     2041 gttacaacgc caacaacgac ctgatgttga ttgataacag ggaaacggct gctacgattc
     2101 ttacgaacca agtccaaata atctttaact gtatctgtct ctctcagaaa accatactca
     2161 tgactagggc gataaagttt ctcaactgtc agaatatcag tcttgatttg gacatttgac
     2221 aaggctttat tgatcatggt cgcgacggta aaggtatcat gcttacttct tagaacagga
     2281 atcccttttt gattggccag tttaagcaca tcatcatgaa cctgaaatcc ccctgtaacc
     2341 agaactgcat tttcattttc caaggctagc aactgaatac gggttcggtc tccgacaatc
     2401 aagagccccc catcatgaag gtaagacaag atattttgtt cagtcatggc accaattgaa
     2461 aacttactaa attctctctc taaaccttct tgcccagcca gaacctcaga agaagtcact
     2521 tctgcaattt cagcaaacgt taatctctct atagcaactt tctgggattt aacacgaatt
     2581 gttccacttc taggacgggt ctccacaatt ccacggtttt cagcttcttt aatagcccga
     2641 taggctgttc catcactaac tcctagatga ttcgaaatgc tacgaacact gaccctttta
     2701 cctactggta attcctccaa atagcttaga atttcctgat gcttactcat tgaactctcc
     2761 ttttacatac cgaaattcaa gataatcccg catttttctt gtgtaacgat cacttccctt
     2821 aaactgacgg aacaaactaa atccagactt aagagcaact ctttggctag cttcattttc
     2881 aaggtgggta atgatagata attgttttaa gccaaattcc tcaaaagaaa gctgacaaat
     2941 ttttctaaca acctctgtca taaatccttg cgaccaagca tcttttctca aaaaatagcc
     3001 aagctcagct tcttttttga tttcatctaa cttctcaaat ttaatagaac caatcatttg
     3061 ttgatttttc tggtcacaaa ttgcccacac tcccaaaggg gacttcataa agtaattggc
     3121 cagtgcatat tgactttctt ccagacttgc ctgcgttggg aaaataaatt gaagattttc
     3181 tggatttgaa gctatctcgc ggaagtcctg actatcacta aaaaagaaag gacgcaaata
     3241 caagcgatcc gtttcaaaaa aagaaaacat tgctaatttg gtccaaatat tcataaacac
     3301 ctctacggtt taatcctcaa caagtctaat atccgctcct aaattacgta atttttcgat
     3361 aatatcagaa taaccacgta agataaactc gatattggta atttcagttt taccttcagc
     3421 cataagccca gcaatgacta gtgcagcccc agctcttaag tcggtcgctt taacactggc
     3481 cccacgtaaa tcacgtccac ccgtgtacaa aatatgacca tttgttgtcg aaatatccgc
     3541 atccatcttt gctagttcaa aaacatgatt tacacgtttt tcgtaaatcg tatcgacaat
     3601 tgtaccacga ccattcgctc ttagtaaaag aggggtaagc ggttgttgca aatcagttgc
     3661 aaagcctggg taaggagctg tcttaatatt gattgctttc aaattagact gttcctcgac
     3721 aaaaatgctg tcttcagata cagtcattct cactcccatt tcttccaact tagcaataaa
     3781 cccttccagg tgttcgtaaa gaacattatt tatacgaatt cctttaccaa ctgcagcagc
     3841 taaagatata tatgttccag cttcaatgcg gtctggaatc acctgatgac gtgtcccatg
     3901 taatctttca acaccatcaa taatgatgat attagttcct gccccacgga tatgggcacc
     3961 catattattc aagagagtag ctacatcaat aatctcaggt tcacgggctg cattttcaat
     4021 aatagtacga ccatttgctt taaccgcagc aatcatcgta ttaatcgttg ctcccacact
     4081 aaccgtatcc atgtaaatac ttgcaccatg aagtcctgta tctttagcag ataacttcat
     4141 gttatctccc tcgtagctag cagtggcacc catagcttca aacgccttaa ggtgtaagtc
     4201 aatcggacga ggaccaagat cacatcctcc cggtagacca actgtcgctt caccaaaacg
     4261 gcctaagagg ctcccataaa aatagtaaga tgcacgaaga ctgttaattt taccataagg
     4321 cattggaata ttttgaacac ctcttggatc aatctccaag acatcgtcat aacgcttaac
     4381 agtagctccc atcaattcca tgatttcgac aagactggct acatccgaaa tatctggaac
     4441 acaatccaaa gtcaccacat catcagccaa gataatagct ggaattaagg ccacaacact
     4501 atttttagca ccactaatag taatttcacc ttgcagtggt aatccaccat tgataacaat
     4561 ttttctcatt ctaagttttc aatactcttt caagatttct aataaggtct tcaaaataaa
     4621 gtatatcata aatttatcct tttttccatc cttttcaatt ttattggata aatcagagtt
     4681 gacgagttag taactgaatt actaatactc aataaaaatc aaaga
//
LOCUS       DAVBDG010000098         4452 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBDG010000098 DAVBDG010000000
VERSION     DAVBDG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.100"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(55..288)
                     /locus_tag="VQ526_001965"
     CDS             complement(55..288)
                     /locus_tag="VQ526_001965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555630.1"
                     /translation="MFSQLRMREEQALLAQDYALETARAGGIEQGLERGKVEGREERK
                     LFAFLDIVRQDLLTSEVASQQLGMSVSEFEALL"
     gene            complement(293..397)
                     /locus_tag="VQ526_001966"
     CDS             complement(293..397)
                     /locus_tag="VQ526_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2555631.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGG"
     gene            complement(612..962)
                     /locus_tag="VQ526_001967"
     CDS             complement(612..962)
                     /locus_tag="VQ526_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2555632.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            1218..3851
                     /gene="polA"
                     /locus_tag="VQ526_001968"
     CDS             1218..3851
                     /gene="polA"
                     /locus_tag="VQ526_001968"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2555633.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3936..4373
                     /locus_tag="VQ526_001969"
     CDS             3936..4373
                     /locus_tag="VQ526_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2555634.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLKAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         1215 a          830 c         1068 g         1339 t
ORIGIN      
        1 aggctccata atatccatag gggatttacc cactatggat attatggagc cattttacaa
       61 cagtgcctca aattcagata ctgacatacc taattgctgg ctggcaacct cagaagtcag
      121 aagatcttgg cgtactatgt ctaggaaggc aaaaagtttc ctttcttccc ttccttcaac
      181 tttcccacgc tctaagcctt gttcaatacc tcccgcccta gcagtttcca aggcatagtc
      241 ctgcgctaac aaggcttgtt cttctcgcat acgtagttga ctaaacattt tcctatcctc
      301 ctcggaccag ctcttgtagt ccagcagttg atttgcttgg gtaatggctc gttgcggttg
      361 ctgggtaaag ggcttgttgc cgaaaaactc caaccatggc ttgcgaacct tgtctttgct
      421 ggtttctctg tattttttaa ttccaagaat gccatcttaa ccagatggtt ttcctgtcca
      481 ttgttggtaa tcgccaatac ctcacctgtt gtgtcttcgc gcatactaaa gctatgaaaa
      541 gccaggtcat ctgagaaata attactatcc acgatagcaa tgacgtaaac aggagcgatg
      601 tgtttgtaga tctagtgagt atcaccttct cgctgacgaa ttttttcaag attttgatta
      661 acctgactgc acaggtaagc ccacaagtga ttgatgaaaa aattctgatg atggacttga
      721 atctcaataa ttacttgagt accgttatcc aactccgcca agacgtctat actggtataa
      781 aaatcctgca ccgagtaagg catggagagt aatacgtgaa tatcgcttcc ctccaaaatg
      841 gtcacatttt ttgctggcaa gtccagcata tcgcgaataa attgacaagt gatttctgga
      901 ttgctgaata ttttcttagc aatcaaatca ttagttgggc tgatgcccgg atgtctgaga
      961 atcctcctat tcctccttct atagtgagtt gaaataagat gtgaataact ctatcaggaa
     1021 agtcaaatta atttatagaa atattttagc agtcaaggtg tactattata gattcaatat
     1081 actatattat agcacatttt taaatctagg tttactaaat tctctggctc tatctattta
     1141 ttcggaaaaa gtttgaaaaa tacttgctct agctcttccc aatggtattt tttgattctt
     1201 tcctttataa tgggtgtatg gataagaaaa aattattatt gattgatggg tcttctgtag
     1261 cttttcgggc gttttttgcg ctgtatcagc agttggaccg ttttaagaat gcggctggtt
     1321 tgcataccaa tgcgatttat ggttttcagt tgatgttgag tcatttgttg gagcgggttg
     1381 agccgagtca tattttggtg gcttttgatg cgggaaagac gaccttccgg acagagatgt
     1441 atgcggacta taagggtggt cgggccaaga ctcctgatga gtttcgtgag caatttcctt
     1501 tcattcgtga gttgctggat catatgggga ttcgtcacta tgagctggct cagtatgagg
     1561 cggatgacat cattgggacg ctggataagc tagcagagca ggatggtttt gatattacca
     1621 ttgtcagtgg ggacaaggat ttgattcagc tgacggatga gcatacggtg gttgaaattt
     1681 ccaagaaagg tgtggctgag tttgaggcct ttacgccaga ttacctcatg gaagaaatgg
     1741 gcctcacacc agctcagttt atcgatctca aggcgctcat gggtgataag tcggataata
     1801 tccctggggt gaccaaagtc ggtgaaaaga cgggtattaa gctcttgctg gagcatggtt
     1861 cgcttgaggg gatttatgaa aatattgatg gaatgaagac ttctaagatg aaggaaaatc
     1921 tcatcaatga caaggaacag gcctttttat ctaaaacact agcgaccatt gataccaagg
     1981 caccgattgc gattggttta gaggacttgg tctatagtgg tccagatgtt gaaaatcttg
     2041 ggaaattcta cgatgagatg ggcttcaaac agctaaagca ggctttaaat gtgtcgtcag
     2101 ctgatgtgtc tgagagtttg gattttacta ttgttgacca aatcagtcaa gatatgctga
     2161 gtgaagagtc tatcttccac tttgagcttt ttggtgagaa ttaccatacg gataatttgg
     2221 ttggatttgc ctggtcttgt ggggataagc tctatgccac agacaagctt gagctgttgc
     2281 aagacccgat tttcaaggat ttcttagaaa aaacatctct gagagtttat gactttaaga
     2341 aggttaaagt tcttttgcaa cgttttggtg tggatttgca ggcgcctgct tttgacatcc
     2401 gtttggctaa atacctcctt tcgactgtgg aggacaatga aattgcgacc atcgctagtc
     2461 tttatggtca gacttacttg gttgatgatg aaactttcta cggtaagggt gttaaaaagg
     2521 ccattcctga acgtgagaaa ttcttggaac acttagcttg taaacttgct gttttggtag
     2581 aaacagagcc tattttactt gaaaaactca gcgaaaatgg gcaattagag cttctttatg
     2641 atatggagca acctctggct tttgtccttg ccaagatgga aattgctggg attatggtca
     2701 agaaagagac cttgcttgag atgcaggctg aaaatgagct tgtcattgaa aaactgactc
     2761 aagagattta cgagctggct ggtgaggagt ttaatgtcaa ctcgcctaag cagttgggcg
     2821 tgcttctctt tgagaaattg ggacttcctc tagaatacac taagaaaacc aagacaggtt
     2881 attcgacagc agtggatgtg ttggagcgtc tcgctcctat tgctccgatt gttaagaaaa
     2941 tcctggatta ccgtcaaatt gctaagattc aatctactta tgtaattggc ttgcaggact
     3001 ggattttggc tgatggaaag attcatactc gctatatgca ggatttgacc cagaccgggc
     3061 gtttgtctag tgtggatcca aacttgcaaa atattcctgc ccgattggaa caagggcgct
     3121 tgattcggaa ggcttttgtg ccagagtggg aggatagtgt gctactcagc tctgactatt
     3181 cacagattga attgcgcgtt ttggcgcata tttctaagga tgagcacttg attaaggcct
     3241 tccaagaggg ggcagatatc catacttcga cagccatgcg ggtctttggc attgagcgtc
     3301 ctgataatgt gactgcaaat gatcgtcgca atgccaaggc agttaacttt ggagtggttt
     3361 atgggatttc agactttggc ttgtctaata atttgggaat tagtcgtaag gaagccaaag
     3421 cctacattga tacctacttt gaacgttttc cagatattaa aaactacatg gatgaagtgg
     3481 tgcgggaggc gcgtgataag ggctatgtag agaccctctt taagcgtcgc cgtgagttgc
     3541 cagatatcaa ttcgcgcaac ttcaatattc gtggttttgc ggagcgaact gctatcaact
     3601 cacctatcca gggttcggca gcagatattc tcaagattgc catgattcag ctggataaag
     3661 ccttggttgc aggtggttat cagactaaga tgctgttaca agtgcacgat gaaatcgtcc
     3721 ttgaagtgcc taaatctgaa ttggtagaga tgaaaaaatt ggtgaaacaa accatggaag
     3781 aagccattca actcagtgtt cctcttatcg cagatgagaa tgaaggggca acctggtacg
     3841 aggctaaata aaaagggggc tagtcctcct tttttgtagt agaattctgc aaaccttttc
     3901 aaaatgtgct atactgatga aaaaggaggt tttctatgag tcaagaattt atcaatccaa
     3961 gtgatggcgt gattcgtcag tatctcgcaa cgagtaaaac ccttgctgtg gtgggtttgt
     4021 ctgaccgtga agaaacaacc agcaatcgag tgacaaagga aatgcaggct cggggctata
     4081 aaatcatccc agtcaatccc aaggcggcag gtggcgaaat cttgggtgaa aaggcttatg
     4141 ctagcctcgc tgaaattcct tttcctgtag atattgtcaa tgtttatcgt cgcagtgaat
     4201 ttctgcccga tgtggcgcgt gattttctca aagctgatgc caagattttt tgggcacagc
     4261 taggacttga aagtttaaaa gcgaaagaga ttttgcgtga tggtggatgt gatgatatcg
     4321 tgatgaatcg ctgtatcaag agagaacata ctcgcttgat tgaggaagca taagaaaaag
     4381 gtagctgatg ggctaccttt tgtgttatac tcaatgaaaa tcaaagagca aactaggaaa
     4441 ctagccgcgg gc
//
LOCUS       DAVBDG010000099         4445 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBDG010000099 DAVBDG010000000
VERSION     DAVBDG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.101"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(45..278)
                     /locus_tag="VQ526_001970"
     CDS             complement(45..278)
                     /locus_tag="VQ526_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2555635.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(284..1276)
                     /gene="plsX"
                     /locus_tag="VQ526_001971"
     CDS             complement(284..1276)
                     /gene="plsX"
                     /locus_tag="VQ526_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2555636.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDIGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1273..2043)
                     /gene="recO"
                     /locus_tag="VQ526_001972"
     CDS             complement(1273..2043)
                     /gene="recO"
                     /locus_tag="VQ526_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2555637.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2040..3209)
                     /locus_tag="VQ526_001973"
     CDS             complement(2040..3209)
                     /locus_tag="VQ526_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2555638.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKVVILNYPANPTGITYSREQLETLAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQTIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3358..4368
                     /locus_tag="VQ526_001974"
     CDS             3358..4368
                     /locus_tag="VQ526_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2555639.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNALESATIVKLT
                     RTLAIIPITIFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
BASE COUNT         1168 a         1148 c          837 g         1292 t
ORIGIN      
        1 atttctaaaa accatctact tccgttgact tggaactccg attgctattt tccttgaatg
       61 attttaacca catctcctac gttttggagt tggtcaattt cttcatcgct gatttcgata
      121 ctaaattcat cttccagagt caagataaac tccatcaagt caacagaatc cgcatccaaa
      181 tcgtctttca gactcaagga ttctgtcacg acaaagtcct ctccctgtcg ctcttggata
      241 atggtcacaa tacggtcaaa aatttctttt tctgtcatct cttttattct cctgaaaatt
      301 cacgcgcagt ctgggcaacc acgtctgttt ctagcatggt acggatctga cgaatcgtac
      361 tataaacagc cttggcatcg cttgagccat gagtcttgac aacaggtgcc ttaacaccaa
      421 acaagaccgc tccaccaata tctgaataat tgagttgttt tttcaaacct ctgaggctgt
      481 ccttgagaag gagggcacct agtttcgctc gaagaccacc acctgtaata gctgtcttga
      541 gcaagcccat gattcccata gctgtccctt cgatggattt gagcacagcg tttcccgtga
      601 aaccatctgc cacaacaaca tctgcaacgc cattcatcaa atcacgcgct tccacatttc
      661 cgataaagtt caaactttca tcagccacta gtaattcata agtttcctta cgaagcggat
      721 cgcccttgct actctctgtt ccgttattga gcaagccaac acgtggttgc gcaatgccac
      781 gaacattttt agcatagaag gatcctagaa ccgcgtattg atggaggtgc tgggctgtat
      841 tttccgcatt ggcaccgagg tctagcatgt caaatccctt cccatctaca gtcggcaaag
      901 tcgacataag cccaggacgg tcgatattct tgatacgacc cacgatgaag aatccagcag
      961 ccaacaaggc acctgtattc ccagccgaaa ggacagcgtc tgcttcacca tctttgacag
     1021 ccttggctgc caataccata ctggcatttt tcttattcct aatagctctc gtaggttcat
     1081 catccgaatc aatcttctca tccgtatgga taatgctgac gcgctctgtc gctgtcagat
     1141 attgcttgat tttagcttca tctccgtaaa gttgaacctc gatatctgaa aagtcagata
     1201 gggcttgatt gacaccctca acaatggcct gaggtgcgta atcgcccccc atggcatcta
     1261 ctgcgatttt tttcatttct tttcctcttt tagtaattgt ccccagtctg ctagggaatc
     1321 aataaatttc tttgatttta ggtgaatccc aacgtactct tcatataatt gatccataaa
     1381 ttggcgtagc tcttgcttga ttccaggctt gagcgaaatg gtctccaaag tctcaaaatc
     1441 aatagcttga aattgattga gcagataggg gatattggga ttgagatgac aacgtctctt
     1501 atcctcatga taatgctctg gacagaggca ggctccatat ttgaaagaaa agtcaaaagc
     1561 ctgaccaacc cgatggcaga agacacactc attaaaattg aggctgattc caaatcgagt
     1621 caaaatttga atttcaaaaa tattggtcaa aacctgataa tccaagcctg cttccatcaa
     1681 ctccaaagtc ttttgcaaaa aagcaaacaa gggagcatcc tgctgattgt cctgcaaact
     1741 agcatctgca agagctgcca cataggtcgc ataggccatg acaaagaggt cactattaat
     1801 cttgggaaaa gtcatgacct catgatagtc ttcgatgtaa ctgagtccgt catcattgat
     1861 tcgcaagaga aatcgtgcca gcaccaaggg ctgaataaca ggcgccagct tagactgacc
     1921 agcgtgtttg acaaaaaaca tgcgtttgcc aacctgctct gtaaaaattt tgacgagctt
     1981 gtcatcctca cgaaaattgc gattgtaaag caccaagcct tgactcgtga tagactgaat
     2041 catgcttctc tcatgtactc ctcaagtcgt ttcatggctt ctttgatagt ctccatgctg
     2101 gctgcataag ataggcggac gtagccttcc ccgtaacgtc caaaggctgc accagggata
     2161 aaggcaacgg ccttcttctg agcaaaatcc ttcagaaaag caaaggagtc ttgattgtag
     2221 cccgctggaa ttttagcaaa aatatagaag gcaccgtctg gtttgataat ctcaaaacca
     2281 agagcagtca ttttttcgat gatatagtcc cgacgttgga tatattcctt cttcatgggc
     2341 tccgcatcgt ttttaccagc cgtcaaggct tctaccgcag catgttgcgc catggtattt
     2401 gcggcagtga ccaagtactg gtgactcttg attaactggg ctgtgaaggt cgcaggagcg
     2461 aaaatcagcc ccaaacgcca acctgtcatg gcatgcgatt tagacaaacc attgataata
     2521 atagtctggt ctctcaacat cgttcctaga gacacatggg cttcgcctgt gtaggtcaat
     2581 tctgagtaaa cctcatcaca gacaacaaaa atttcatact tgcgtaaaac agctgccaag
     2641 gtctctaact gctctcgact gtaggtaatt cctgtcggat tggctggata gttgagaata
     2701 accaccttga gcttatcacc ctgctccaaa atggccttct ccaacatctc aggagtcaag
     2761 acaaaaccat tttcagtcgt atcaatctca acaatttctg ccccaactaa gttaacaatc
     2821 ggttcatagc ctggataagc aggagctggc aaaagtacct tgtctccctc ttccaaaata
     2881 gccgtcaaag tcgcagataa agcctctgtc gccccaattg taaccaagat ttcattttca
     2941 ggagcatagt ccagttggta cttttcctta acaaagtcac tagctgcctg acgtagagtc
     3001 agcagaccac tcatccctgt atagtaggat tggttctgat caatcgctcg cttggccgcc
     3061 tccttgacat ggtctggcgt tgtaaaatca ggttccccca aggtcaaacg caagacccca
     3121 ggaatctccg aaatagcctg gtcaaactga cgaatcaacg aaacttgaat tttatctaac
     3181 tgtttattaa agcgcttagt taagtccata gatacctcct actttcaaaa cgtatctcta
     3241 ttatactaca aaagcctccc gaccgcaaaa atacattaga gaatagcttt ccactttcta
     3301 ttttactttt cctctttaca ggtctataat ggtaacagat tctatttgga ggtttttatg
     3361 tcatttctat caaaaaatgg agcaggcatc ttggtctgcc ttctcatttc tatcgtatct
     3421 tggtacttag gaggattctt ccctgtgatt ggcgcacccg tttttgcgat ttttatgggc
     3481 atgctcctac atccctttct ctcgtcttat aaacaactgg atgctggatt gacctttagt
     3541 tccaagaaat tgctccagta tgccgttatc ttgcttggtt ttggtctcaa tatctctcaa
     3601 gtcttcgcag ttgggcaatc ttcactccct gtcatccttt ccaccatttc aatagccttg
     3661 attgttgcct acctcttcca gcgcttcttt gccctggata caaaactggc taccttgatt
     3721 ggagtaggtt cttctatctg tgggggttct gccattgcag cgacagcgcc tgttatccat
     3781 gctaaggaaa aggaagtagc ccaagccatc tccgttatct ttttcttcaa tgtcttggct
     3841 gcgctcatct ttccaaccct cggcacctgg cttcatctat ccaatgacgg cttcgccctc
     3901 tttgcaggaa ctgcggtcaa tgatacttcc tctgtaaccg ccacagctag cgcttgggac
     3961 agtctctacc agaccaatgc cctcgaatcc gcaaccattg tcaaactcac gcgcacccta
     4021 gctatcatcc ccatcactat ctttctctcc tactggcaaa gtcgcgagca aaaaaataag
     4081 caaggcttac aactgaaaaa agtcttccca ctttttatcc tttacttcat ccttgcatcg
     4141 ctcctaacca cccttctgac ctctctcggt gtgtctagta gtttctttac ccctctcaaa
     4201 caactctcta aattcctcat tatcatggcc atgagtgcca tcggtctcaa aactaatctg
     4261 gtcgctatgg tcaaatccag tggaaaatcc attgttctcg gagccgtctg ctggattgcc
     4321 atcatcctca ccagccttgg tatgcagacc cttatcggca ttttctaata ctcttcgaaa
     4381 atctcttcaa accacgtcaa cgtcgccttg ccgtatatat gttactgact tcgtcagttc
     4441 tatct
//
LOCUS       DAVBDG010000100         4403 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBDG010000100 DAVBDG010000000
VERSION     DAVBDG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.102"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            468..1589
                     /gene="mnmA"
                     /locus_tag="VQ526_001975"
     CDS             468..1589
                     /gene="mnmA"
                     /locus_tag="VQ526_001975"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2555640.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1730..2185
                     /locus_tag="VQ526_001976"
     CDS             1730..2185
                     /locus_tag="VQ526_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2555641.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2195..4108
                     /gene="mnmG"
                     /locus_tag="VQ526_001977"
     CDS             2195..4108
                     /gene="mnmG"
                     /locus_tag="VQ526_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2555642.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         1274 a          895 c         1073 g         1161 t
ORIGIN      
        1 aggtgccctt gcagtcaaca caactgtaga tggctatgaa gtcaatgcca atggtgaatg
       61 ggtttaagcc gattaaatta aatcatgtta agaacatttg acattttaat tttgaaacaa
      121 agataaggtt cgattgaata aatttatgtt cgtattcttt aggtacctat cttatgattt
      181 caggaaatgt cattaaaaaa acgactcctt ttctctaact tgaaaaatag attagagaaa
      241 atgggttgtt ttatttatta tagttatttg aatgaagata agaagaaggt atactcacat
      301 cattcacata atctgtatat tgactataag ttttaaaaaa caatttttaa gctcttcctt
      361 gtcttctcta accaagcgtg ttataatgaa tactgctcaa gcgaccttca atcgtgaagc
      421 acacacgacc ttcaatcgtg aataaacgaa tagatgggag acttaccatg agtgataact
      481 ctaaaacacg tgttgtcgtg gggatgagtg gtggtgttga ttcgtcggtg acggctcttt
      541 tgctcaagga gcagggctac gatgtgatcg gtatcttcat gaagaactgg gatgacacag
      601 atgaaaacgg cgtctgtacg gcgaccgaag attacaagga tgtggttgcg gtggcagacc
      661 agattggcat tccctactac tctgtcaatt ttgaaaaaga gtactgggac cgcgtttttg
      721 agtatttcct agcggaatac cgtgcagggc gcacgccaaa tccggacgtt atgtgcaaca
      781 aggaaatcaa gttcaaggcc tttttggact atgccatgac cttgggggca gactatgtag
      841 cgactgggca ttatgctcga gtggcgcgtg atgaggacgg cactgttcac atgcttcgtg
      901 gcgtggacaa tggcaaggac cagacctatt tcctcagcca actttcgcaa gaacaacttc
      961 aaaaaaccat gttcccacta ggacatttgg aaaagcctga agttcgaaaa ctagcagaag
     1021 aagcaggcct ttcgactgct aagaagaaag actcgacagg gatttgcttt atcggagaaa
     1081 agaactttaa aaacttcctc agcaactacc tgccagctca gcctggtcgc atgatgactg
     1141 tggatggtcg cgatatgggc gagcatgcag gtctgatgta ctatacaatc ggtcagcgtg
     1201 gcggactcgg tatcggtggg caacacggcg gtgacaatgc cccttggttc gttgtcggaa
     1261 aagatctaag caagaatatt ctctatgtag gacaaggatt ctaccatgat tcgctcatgt
     1321 caactagcct agaagccagt caagtccact ttactcgtga aatgccagaa gagtttacgc
     1381 tagaatgtac ggctaaattc cgttaccgtc agcctgactc taaggtgacc gtccatgtca
     1441 aaggagacaa ggcagaggtc atctttgcgg aaccacaacg cgcgattaca ccaggccagg
     1501 cagttgtctt ttacgatggc gaagagtgtc taggtggtgg tttgattgac aatgcttacc
     1561 gcgatggaca agtttgtcag tacatttaga ttgacaaatt ttctcaattt gctacaataa
     1621 taacagcaat agaaatgatg gtcaaagctc atggatgttg caggcttttt tgtcctgcac
     1681 ttctttggag ttttgactgt ttttgtgtcg tttaagggaa aggacaagaa tgactcaaca
     1741 agactttcgg acaaaagtag acaatacggt ttttggtgtt cgggcgacag ctttgattct
     1801 tcaaaatcac aagcttctag tcaccaaaga caagggcaag tattacacta tcggcggtgc
     1861 gattcaagtc aatgaaagca cggaagacgc ggtagtccgt gaagtgaagg aagaactggg
     1921 tgtcaaagct caagctgggc agctagcttt tgtggttgaa aatcgttttg aacaggacgg
     1981 tgtttcctat cacaatatcg agtttcatta tctggtggac ttacttgagg atgccccatt
     2041 gaccatgcag gaagatgaga aaaggcagcc ctgtgagtgg attgacttgg ataagcttga
     2101 ggatatccag ctagttccag cctttttaaa aacggcccta ccagattggg aaggccaact
     2161 aagacacatt catcttgagg aataggagag aaacatgact tatcatttta ctgaagaata
     2221 cgatattatt gtaattggtg cgggacacgc tggggttgag gcttccttgg ccgctagccg
     2281 tatgggctgt aaggtcctgc ttgcgaccat caatattgaa atgctggctt tcatgccttg
     2341 taatccctct atcggtggtt ctgctaaggg gattgtcgta cgtgaagtcg atgccctcgg
     2401 tggcgagatg gccaagacca ttgacaagac ttacatccag atgaagatgc tcaacacagg
     2461 gaagggccca gccgttcgtg cccttcgtgc gcaggctgat aaggaacttt actctaagga
     2521 aatgcgcaag acagttgaaa atcaagaaaa tctgaccctt cgtcaaacca tgattgatga
     2581 gattttggtg gaagatggca aggttgtcgg tgtgcgtaca gccacccatc aagaatatgc
     2641 tgctaaggct gttattgtga cgacagggac tgctctccgt ggggaaatta tcatcggaga
     2701 cctcaagtac tcatcaggtc ctaaccacag cttggcttct attaacctag ctgacaatct
     2761 caaggaactg ggtctcgaaa tcggtcgttt caagacagga acccctccac gtgtcaaggc
     2821 ttcttctatc aattacgatg tgacggaaat tcagccagga gacgaagtgc ctaatcattt
     2881 ctcatacact tcacgtgatg aggattatgt caaagatcaa gtgccatgct ggttgaccta
     2941 taccaatggt accagtcatg agattatcca aaacaacctc caccgtgcgc ctatgtttac
     3001 aggtgtggtc aagggagtgg ggcctcgtta ctgtccgtcg attgaagaca agattgtgcg
     3061 ctttgcggac aaggaacgtc accaactctt ccttgagcca gaagggcgca atactgagga
     3121 agtctatgtt caaggacttt caaccagtct gcctgaggat gtccagcgtg acttggttca
     3181 ttccatcaaa ggtttggaaa atgcagagat gatgcggaca ggttatgcta ttgagtatga
     3241 tatggtcttg cctcatcagt tgcgtgcgac tttggaaacc aagaaaatct caggtctctt
     3301 cactgctggt cagacaaatg gaacatcagg ttatgaagaa gctgctggcc aagggattat
     3361 cgcgggtatc aatgcggctc tgaaaatcca aggtaaacct gagttgattc taaaacgaag
     3421 tgacggttat atcggggtga tgatcgacga cttggtgacc aagggaacca ttgaacctta
     3481 ccgtctcttg accagtcgtg ctgaataccg tctcattctt cgtcatgaca atgctgatat
     3541 gcgcttgact gagatgggac gcgagattgg ccttgtggat gatgaacgct gggctcgttt
     3601 tgaaatcaag aaaaatcaat ttgataatga gatgaaacgc ctagacagta tcaaactcaa
     3661 gccagtcaag gaaaccaatg ctaaggttga ggaaatgggc ttcaagccgt tgacagatgc
     3721 ggtgacagcc aaagaattcc ttcgccgtcc agaagtttct taccaagatg tggtggcctt
     3781 catcggacca gctgcagaag acttggatga caagattatc gaattgattg aaacagaaat
     3841 caagtacgaa ggctatattt caaaagccat ggatcaggtt gccaagatga aacgtatgga
     3901 agaaaaacgc attccagcca atattgactg ggatgacatc gattctattg cgacggaagc
     3961 tcgtcagaag ttcaaactca tcaatccaga aaccatcggc caagccagcc gtatttcggg
     4021 agtaaaccca gcagatattt ctattttgat ggtgtatctg gaaggtaaaa atcgtagtat
     4081 ttctaaaact cttcaaaaat caaaatgata cgtcgtcggc ttcttacgaa tgagttcaaa
     4141 gcttggcttt gattcatctc cagcctccca tagttccccg aactatggga gctaactcaa
     4201 gttatgcatt ttcctagcct tctggtctga ttttcattga gttttaatct caaaagaaag
     4261 catagagaaa aacagctccg aagacggagc tgttttgttg acttgtactc aatgaaaatc
     4321 aaagagcaaa ctaggaagct agccgtaggt tgctcaaagc actgctttga ggttgtagat
     4381 agaactgacg aagtcagctc aaa
//
LOCUS       DAVBDG010000101         4385 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBDG010000101 DAVBDG010000000
VERSION     DAVBDG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.103"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(73..1176)
                     /locus_tag="VQ526_001978"
     CDS             complement(73..1176)
                     /locus_tag="VQ526_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2555643.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYVASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            complement(1193..1990)
                     /locus_tag="VQ526_001979"
     CDS             complement(1193..1990)
                     /locus_tag="VQ526_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2555644.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            complement(1977..2654)
                     /locus_tag="VQ526_001980"
     CDS             complement(1977..2654)
                     /locus_tag="VQ526_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2555645.1"
                     /translation="MISKRLELVASFVPQGAILLDVGSDHAYLPIELVERGKIESAIA
                     GEVVEGPYQSAVKNVEAHGLEEKIQVRLANGLAAFEEEDRVSVITIAGMGGRLIARIL
                     EEGLDKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            3204..3672
                     /locus_tag="VQ526_001981"
                     /pseudo
     CDS             3204..3672
                     /locus_tag="VQ526_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3666..4196
                     /locus_tag="VQ526_001982"
     CDS             3666..4196
                     /locus_tag="VQ526_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HET2555646.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPSWKMDLFSEEEKRIYFQTTCEVS
                     SKDSAINKYKIEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDI
                     KNEAYMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSDIHKLGI"
     gene            complement(4271..>4385)
                     /locus_tag="VQ526_001983"
     CDS             complement(4271..>4385)
                     /locus_tag="VQ526_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2555647.1"
                     /translation="KQLEQAIVDYIDYYNNKRIKVKLKGLSLVQYRTKSFG"
BASE COUNT         1361 a          924 c          805 g         1295 t
ORIGIN      
        1 cacaacctca aagcagtgct ttgagtaacc cgcggctagt ttcctagttt gttctttgat
       61 tttcattgag tattattcgc tttttactcg tttgacatag ttttcaattg ggtaatttag
      121 agggtccaag gtcaactcct tgtcttggat cagttgggct agatggtaac caatgatagg
      181 accagttgtg aggcctgatg aacctagtcc actggctaca tagacacctg ccaattctgg
      241 cacctgccca aagaagggag agaaatcact ggtataggca cggattccca cacgctcagc
      301 ccttgaagtc gctctagcta aaactgggta atgaggcaag gccgcctcct ccatttgttg
      361 gagcaagggt tcatctaccg tcaaatcaaa tcccatgtca ttttcatggg tagcgcccaa
      421 ggataatttc ccacctgcaa agggaatcaa atcccactcc ccttctggca tgacaacagg
      481 gtaatcttcc atgtcttggg caagctgata atctcgtagt tgtccttttt gaggacggac
      541 atccacttca taacctaaag gctctaacat gtcccccaac caagctcccg ttgccaaaat
      601 aacctgatca aacacctctt cgccaatctg gtagcctgat gataagagtg tcagagtcac
      661 tttttctttg accagcttga catgactggc ttctagcaga cgagttacca agagttggcc
      721 atctactctc gctccaccag aagcatagag caggcggtca aatccctgca aaccagggaa
      781 taattcatta gctgaagctt ggtccagaat ggctaattgc cctatcaagg gagattcttc
      841 tctgcgctgg agggccagtt gataaagttc ttccaacttg gattcatcct ttttcaagag
      901 aaagactccc gaacgctggt aaaagtcgat tttttgtcct gatttctcta aatcagctaa
      961 taaatccaca taaaagtcag cccccaagcg cgccatcttg taccaggctt tattacggcg
     1021 tttggaaaac caaggactga taattcctgc tgcggccttg gtggcttgac cttgctcatg
     1081 gtcaaaaacg gtcacctcta ggtcactttc tctcgagagg tagtaggcag ctgttgctcc
     1141 cacaattcct gctccaataa tggcaacttt tttcattgtc ttcactttct aactagatat
     1201 gacggaaagg attggtcgat gcttgactag gtaaaatctc tagttcccag ccttttttct
     1261 ctttccattg agtaaggagt gctgcgattt tttccacaaa aagcacttcg atatagtgac
     1321 ctggatccaa tgctaacaag ccatcagaca gcatatcctg agcagtatgg tagtagatat
     1381 caccagtgat atagacatct gccccctttg ccaaagcatc cttatagaaa gactgcccgc
     1441 ttccaccaca aattgctact cttgaaatag gcttctgcaa atcattctct tgataatgca
     1501 ccattcgaag gctatctagg tcaaagactt gcttgacctg ttgggccaat tcccaaaatg
     1561 tctgaggctg aatattccca atacgtccaa ttccacgttc tggacctgtt tcctgcagat
     1621 aagtcgtctc ctcgattcct agcatctgac aaaaccagtc attgagccca ttttcaacga
     1681 tatcaatatt ggtatggctg acataaactg cgatgtcatg cttaatcagg tcgatgtaaa
     1741 tctgattttg cggacggctg gcaagcaagt ccttgatagg acgaaagata ggcgcgtgct
     1801 tgacgataat caagtccaca cccttttcaa tggcctctgc cactgtctct tcacgaatat
     1861 cgagggcaac catgaccctt tggataccct tgtctaaagt gccaatttgc agaccacggc
     1921 tgtctccctc catagaaaat tcctgagggc aaaaggcttc ataagcttgg atcacttcac
     1981 ttgctaacat ggagcacctc cttgatagct tgaatcttat ctactagaac ttgacgttct
     2041 tccagatttt tttctgggat ttgtccgagg gcgaactcta gcttctcagc ttctttttgc
     2101 catttttgga caaatactgg actgacttct ttggacaaga agggaccaaa gcgaacatca
     2161 ctggctgata gcttcatttg tcctgcttcc accaccaaaa tctcataaaa ctttccagct
     2221 tcttctaaga tgctttctgc tacaatctgg aatccatgat cctgtagcca gatacgcaag
     2281 tcgtcttcac gattattggg ctggagaatc aaacgctcta cgttagctaa cttgtccaag
     2341 ccttcttcta aaatcctagc aatcaaacgg ccgcccatcc ctgcgatggt aatgacagac
     2401 actcggtctt cctcttcaaa agctgccaaa ccattggcta aacggacttg gattttttct
     2461 tccaagccgt gggcctcaac atttttgaca gcagactgat aaggtccctc cacaacttca
     2521 cctgcaatgg cactttcgat ttttcctctt tcgactaact ctataggcaa ataagcatgg
     2581 tcactcccca catctagtaa aatggctccc tgcggcacaa aggaagctac caactctaat
     2641 ctctttgaaa tcatcttctc tctctttcca aaactctatt acctcttatt ataccacatt
     2701 tcaatcttca acttcccagt aatataagca cctctggcga aagaagcttc aatgtcctaa
     2761 agtaataagt gaatccaatt gaaagatttt aaacaatttg caaaaatgtc aaaaaaataa
     2821 aaaataaaca gtttattcag aaaattcttg acatataaaa acacatggta gaatataatt
     2881 agaaagttag aaaaaataaa agtttgacta aaatttgtat ttgaaggtgg tgttcagata
     2941 agaaatttag tcagacgaac cacgaatttg ctctatgctt tctggaattt atcataacag
     3001 gaggatacag tcatggaaca aacattgttt gaattagaac tacttccaga ggaagatatc
     3061 attgtcacag gtctccctaa gtattgttct tttacttgtt taattacagg tcgctagtta
     3121 tattttatat aaaataagta gctttactta cggaataggc tagtgctgtg tctctagcct
     3181 attttaataa ttaggagttt gttatggatt tattagagaa agaatgttta aaatgtgata
     3241 aaaatttcca acagggtgat atttggaatt actattattt atcagataag gtgcctgcac
     3301 aagggtggaa aatacacata agctcccaaa taaaagacgc tgtaaatatt tttaagattg
     3361 tgtataaact atcccaacta aataattgta gctttaaagt tgttaaaaat ttagaggaat
     3421 taaaaagaat taattcccct agggaaatga gccctactgc taacaaattt ataactctat
     3481 atcctaagtc agaatctgaa gctaagagta tgatttgtaa tcttacgaat agactgtcag
     3541 aatttaaggc tccaaaaata ctatctgact atcaatgtgg aatgcattct ccagttcatt
     3601 atagatatgg ggctttttta aaaaaacaag cttatgatga aaaaaataaa aaagtcatct
     3661 atttattgct agatgaaaaa aggaagaact atgtagaaga taagagacaa aatttcccta
     3721 gtcttcctag ctggaaaatg gatttatttt cagaagaaga aaaaaggatt tattttcaaa
     3781 ccacatgtga agtttcaagt aaggactctg caataaataa atacaaaata gaaaaaatta
     3841 ttaagagatc aaataaaggg aatgtttatc gggcaattag aaaatcggac gggcaaaaag
     3901 taatcatcaa gcagtcaaga ccttttgtga attatgatgc cgaaggagaa tggacagcac
     3961 ttgatgatat aaaaaatgag gcttacatgc taaaaaaatt agccgataaa agttatacta
     4021 ctaatttaac tgatgaattt tatatcgtgg atgattattt tcttgttcag gaacaagttg
     4081 acgggttaaa ctttgaagaa tttattcgag aaacagaaca ctccttaaat attagagaaa
     4141 aaacactgga taacattgtg aacatagtaa gcgacatcca taaactggga atataaaatt
     4201 gtagatatag ctcctacaaa ttttatatat accaaaaatg tactgccccc caaaaagtta
     4261 gacaattaat ttatccgaag gatttagttc tgtattgcac aaggctaagt ccttttagtt
     4321 ttaccttaat tcgtttattg ttgtagtaat caatatagtc tacaatagct tgttccaatt
     4381 gctta
//
LOCUS       DAVBDG010000102         4287 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBDG010000102 DAVBDG010000000
VERSION     DAVBDG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.104"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..141
                     /locus_tag="VQ526_001984"
     CDS             <1..141
                     /locus_tag="VQ526_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2555648.1"
                     /translation="LLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSC
                     FN"
     gene            complement(134..307)
                     /locus_tag="VQ526_001985"
     CDS             complement(134..307)
                     /locus_tag="VQ526_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2555649.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(466..2073)
                     /locus_tag="VQ526_001986"
     CDS             complement(466..2073)
                     /locus_tag="VQ526_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2555650.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2226..3710
                     /locus_tag="VQ526_001987"
     CDS             2226..3710
                     /locus_tag="VQ526_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2555651.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3805..4287
                     /locus_tag="VQ526_001988"
     CDS             3805..4287
                     /locus_tag="VQ526_001988"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2555652.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1241 a          980 c          816 g         1250 t
ORIGIN      
        1 cttttacctc ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat
       61 atcaaaaagc acctcaaaaa ggtattacca agttgcaata ccttttatga ggctcttttg
      121 tcttgttctt gtttcaattg actatattat cagtgtaata gtctaataga tagttaaatg
      181 tagttggatt tccactaaag gttgatgagt aagtttttgt atctgaacct gattggccgc
      241 aagcagctaa aagcaaaaca gatgcaaaag tcaggcctgc actaaggaca cgcttcttta
      301 tgttcatctt ctttctcctt aatagtggga atttgtaaag ttaattgaat ttcgagaatg
      361 aaagtttata aaccttggtt ataaaaaaca aaggatttct gctttttata cagtcctccc
      421 ttgtttttat atgatttcaa ttttaaattt ttctgcaaaa aatatttata gtaattccac
      481 acagaaagca tcccatggaa ctaagatttg tttttcaaag acttcttgag ctagggtgtt
      541 ttcaatcaag acagatttga cttttccttc tactgtcaag tcttgctctt cattggacaa
      601 gttagccaca actaggaagc gacggtcgcc atccttacgt atataagcaa agaccttatc
      661 agccgtatca agcaattcaa agtcagctcg aactagccag ctattctcct tgcgaatttg
      721 gaccagtttc tgataggtat agaaaataga atctggattt gccagcgctt cttggacatt
      781 gatcatctcg taatttggat taaccgccaa ccaaggttga cctgttgaga aaccagcgtt
      841 tttgctctcg tcccattgca taggggtacg ggcattgtca cgtccaataa cacggatact
      901 gtccatgatt tcttcaatcg gaacaccttt ttcaagagcc tcacgcgcat agttgagaga
      961 ttcaatatct tctacttgat ccagcgtttc aaagggatag ttggtcatgc caatttcctc
     1021 accttggtag atataaggag ttcctctcat gagatgaagc aagatggcaa aggctttggc
     1081 agatttttcg cggtattctt ggtcatttcc ccagattgag acaatacgag ggaggtcatg
     1141 gttgttccag aagagggaat tccagccgtc ctcaactcct aactctgtct gccatttgtt
     1201 gaagatttct tttaacttag cgatattcag ctctttttga tagtgccatt taggctgacc
     1261 ttcctgatac tgaagaccga tatgttcaaa ctggaagacc atagacaatt cttgcccctt
     1321 tggatcagag tagagcttgg caatctctgg cgttgctccc caagtctccc ctactgtcaa
     1381 gagatcctta tctccaaagg tcgcctgatt catttccttg agatagggat ggagcatagg
     1441 accattattg actaccttct cgtcaggaat tttgccaatc atgtcaataa catccatacg
     1501 gaaaccacca atacccttat caatccagaa gttcatcatc tcataaattt tctggcgaag
     1561 tttttcattt tcccagttga gatccggctg tttcttgctg aaaaagtgga gatagtattg
     1621 acctgacttt tcatcgtatt cccaagcaga cccactaaag atagaatcta ggtcattggg
     1681 ttcatcgcgc cagatatagt agtctcgctc agggctatta ggattttcac aggcctctac
     1741 aaaccaggca tgctcatccg aggtatgatt gaccaccaag tccatgatga tacggatatc
     1801 acgcttctta gcttctgcaa tcagttgatc catatcctcc atggttccaa aaatcgttgc
     1861 aatcgcttga taatcagcaa tatcgtagcc attatcatcc atagggctgt cataaacggg
     1921 agaaagccaa attgctgtga ttcctagctt ggctagatag tccaacttac tggtaattcc
     1981 tggcaaatcg ccaattccat ctccattact atccataaaa ctctttggat agacttgata
     2041 gactacggca ttgtgccacc atttttcttg catcttctta cctcattatt ttcataatct
     2101 atagtctatg atagcacttt ttaaaatttt gtgcaagcgt ttgcttaatc tttttaattt
     2161 cttttttagc tccgtagttt cctaacttcc aattttttat tataatagag tccagaggta
     2221 aaaaaatgaa aaaacaagct tttagttctg aacaatattt gaatttacaa cgcgaccata
     2281 ttttggagcg cattaaccaa tttgacggca agctctactt agagtttggc ggtaaaatgt
     2341 tagaagattt ccacgctgct cgtgtccttc ctggttatga acctgacaac aaaatcaagc
     2401 tcttgcaaga attgaaagag caggttgagg ttgtgattgc cattaatgct agcaacattg
     2461 aacattccaa agcacgtggc gacttaggca tttcttatga ccaagaagtt cttcgtttga
     2521 ttgacaaatt caatgaatta gggatttttg ttggttccgt tgtcattaca caatacgctg
     2581 gccaacccgc tgcagatgcc ttccgcaatc aacttgagaa aaacggaatt gattcttatc
     2641 ttcattatcc aatcaaagga tatccgacgg atatggatca catcatttcc ccagaaggca
     2701 tgggcaaaaa cgactacatc aaaaccagtc gcaacttgat tgtcgtaacc gctcctggac
     2761 ccggttctgg aaaattggca acgtgtatgt ccaatatgta ccacgaccaa atcaatggca
     2821 tcaagtctgg ctacgctaaa tttgaaacct tccctgtttg gaatcttccc cttcatcacc
     2881 cagttaactt ggcttatgaa gctgccacag ctgaccttga tgatgtcaac atgattgacc
     2941 ccttccatct tcaaacctat ggagaaacca ctgtcaacta caaccgtgat atcgaaatct
     3001 tcccagtgct caagcgcatg ttggaacgta ttctcggaaa atcaccatac gcttcaccga
     3061 cagatatggg tgtcaacatg gttggtttcg ctattacaga tgacgaggct gctgtcgaag
     3121 cttctaaaca agaaatcatc cgccgttact atcaaacagt tcttgacttc aaagctgaaa
     3181 aagttggcga agctgccgtc aagaaaattg agttactcat gaacgacctc ggtatcacac
     3241 ctgcagaccg taaggttgct gtcgttgcgc gccaaaaagc agaagaaact ggtggaccag
     3301 ccctagcctt tgaattgcca aatggggaaa tcatcactgg taagaactca gaactctttg
     3361 gccctacagc cgctgccttg atcaatgcca tcaaaaaatc agctgacatc gctaaagaag
     3421 taaaactaat cgagcctgaa gttgttaagc caatccaagg tcttaaaatc gatcatctcg
     3481 gtagccgcaa tccacgcctt cattcaaatg aaatcctgat tgcacttgct atcacagcta
     3541 cagaaaatcc tgatgctgcc cgcgctatgg aagaactcgg caacctcaaa ggaagcgaag
     3601 cccactcaac catcatcttg actgatgaag acaagaatgt ccttcgtaaa ctgggtatca
     3661 acgtaacctt tgacccatac taccaatacg accgcttata tcgtaagtaa agatttcaac
     3721 ctctccagcc tcggagaggt tttctctctg tctcaggagc aacctttatg atataatgag
     3781 agaagaaaac tgaaaggatt taccatgtca aaagaagtta ttgtcgaaag ttttgaactt
     3841 gaccacacca ttgtcaaagc accctatgtt cgcttgattg gggaagaaac aggaccaaaa
     3901 ggagacatca tctccaatta tgatattcgc ttggtgcaac caaacgaaga ctctatccct
     3961 actgccggcc ttcacactat cgagcacctc ttagccaaac tcatccgtac ccgcattgac
     4021 ggcatgattg actgttcacc atttggttgc cgcacaggct tccacatgat tatgtgggga
     4081 cgtcacacca gtgctaaaat cgcagctgtt atcaaggatt cgctcaagga aatcgctgaa
     4141 actactactt gggaagatgt cccagggaca accatcgaat cttgcggaaa ctacaaggac
     4201 cacagcctct tctctgctaa agaatgggca aaactcatct tggaacaagg gatttcagat
     4261 gatgcctttg aacgtcatgt gatttaa
//
LOCUS       DAVBDG010000103         4222 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBDG010000103 DAVBDG010000000
VERSION     DAVBDG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.105"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(9..1298)
                     /locus_tag="VQ526_001989"
     CDS             complement(9..1298)
                     /locus_tag="VQ526_001989"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2555653.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRAYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVSQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1395..2624)
                     /locus_tag="VQ526_001990"
     CDS             complement(1395..2624)
                     /locus_tag="VQ526_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET2555654.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SILCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYASGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFFDNS"
     gene            complement(2817..3668)
                     /gene="rgg"
                     /locus_tag="VQ526_001991"
     CDS             complement(2817..3668)
                     /gene="rgg"
                     /locus_tag="VQ526_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HET2555655.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3792..4133
                     /locus_tag="VQ526_001992"
     CDS             3792..4133
                     /locus_tag="VQ526_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2555656.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         1341 a          878 c          832 g         1171 t
ORIGIN      
        1 tttgattttc attgagtata aaatcctagt ttttcaaaga tttctgagaa gttttggctg
       61 attgtctcaa gtgacacttg cacttcttct cgggtttggt tgttcttgac tgtcacttgt
      121 ctgctttcga cttcgctctc tcctagggtg atgagggtct tagccgcaaa gacatcggct
      181 gacttgaact gagctttgag cttacggttg aggtaatcac gctctgcttt gaaaccttgt
      241 tggcgaagag cttggactag ttctagggct ttgacatttg ctccatcacc caagactgcg
      301 atatagacat ctagggcatt ttcgataggg agggccacgc cttgtttttc aaggatgaga
      361 agcaggcgct ctacaccaag cccaaaacca aatccagcag tttcagggcc tccaaagtaa
      421 gcaaccaaac catcgtagcg accacccgca cagactgtca agtcattgcc ctcaatctct
      481 gtgataaact cgaaaatggt gtggttgtag tagtccagac cacgcaccat attggtatcg
      541 atgatgtaat ctactccaag attttccaac atctgactca cagcatcaaa atgagtttgg
      601 ctttcttcat caagaaagtc caagatagac ggcgcattct ctactgccac cttgtcttct
      661 ttttccttag agtccaagac acgaagagga ttttcctcca agcgacgttg gctatcctta
      721 gacaaggtct ccttgagcgg tgtcaaatag tcaatcaagg cttggcggta ggctgcacgg
      781 ctctcaggat ttccaagagt gttgaggtgc aatttgacac cttgaatacc gatttccttc
      841 aaaaaatggg ctgccataac gattgtttcc acatcggtag ctggattgct agaaccaaaa
      901 cactcaacac caatctggtg gaattggcgc aagcgccctg cctgtggacg ctcataacgg
      961 aacataggtc ccatgtagta gaacttgctt ggcttttgca cttctggggc gaagagttta
     1021 ttttccacat aggcacggac aacgggcgca gttccttctg gacggagggt aatatggcgg
     1081 tcacccttgt catagaaatc gtacatttcc ttggttacaa tatctgttgt atctccgaca
     1141 gagcggctga taacctcgta atgctcgaaa ataggcgtac gcacttctgc gtagttgtag
     1201 cgcttgaaaa tttcacgggc aaagccctca acgtactgcc acttagcaga ctcagcaggt
     1261 aaaatatcct gcgttccttt tggtttttgt aatttcatag ggaatcctct ttaaacttaa
     1321 tagtcttatt ttaccataaa tagagggatt aaaacagtaa gaaaaaaatt aggatttaga
     1381 tatcattttt gagattaaga attgtcaaaa aaaatagcta gcaaggaaag accaacaaat
     1441 agcatccaag tcaactgtat attccatacg gctactagtg aaaaacaagc tgttcccaca
     1501 ggtatggata aggtaaacaa tagacctaaa aaattactag tacgagctag aacctctgga
     1561 gctagatttt tcatgagcat ggcactaatc tttggttgaa ctttaccaga cgcatacaga
     1621 gtaaagaaga gaaatagcaa accaagcacg acttgattga ataaattagc caaaccaact
     1681 agactaagcc ctacggtcgc ccacatcatc aatctaggca aggactgctt cccaaaataa
     1741 tcattgcccg taaggctact gatgatgact gctactaaaa cacagaattg attgataaat
     1801 agtgcctctg tataagaaaa attcaagaga gaatggctca aaaagaagat attataaatt
     1861 ccacccaaag cgccacccaa ggaattaata agcaagacag caaagagcat aaaaccaaag
     1921 tttttctgtc cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt taggaactgg
     1981 tctttgatag aaagcttctc attttttaag ttttcaccat cagcagatga cattgacagg
     2041 ctcaatttgc tttttcctaa aaagaggata gtggctgata ctaggaaaaa gcaggcattg
     2101 attcccgcaa cgagagaaaa attgttgacc gatagagcta agagccagac tccgaaagct
     2161 tgaccaccaa tagctgaaat ataggtgatg aactgtgaaa aagaataagc ctccatcaga
     2221 tcatcttcag ctactttttc cttaataaga ggcatacgca ggccacctgc aaaatcactg
     2281 atgatatcac taatgacatt gatcaaacac aggatagaaa aggcaaagag actagcttgc
     2341 tgaacaacta gggctgctag aaaaaataga accgcctgaa acaaaccgct atagaccatc
     2401 catttgacct tgtccctcgt gtaatctgcc cgaatccctg caaaaactgt aaagagggtc
     2461 ggaagaatca tgacaatatt cgccatagca acagcaaaag atgcttgtga caaggtcgat
     2521 gcatagacga taaagaccag gttgaaaatc gaagcaccaa aagcattgaa gaagcgtgat
     2581 aaagtcaaaa ttctataagc tggatttcta aacaatagtt tcatagataa tctcccttct
     2641 tgttcgaacc tctatatgaa gtatgactat agtatagcac ttagaaattt gagagggaga
     2701 actcttgaat atatgcaaca aataaaatga gcaaaaagaa acgatagaac ttttgtaatg
     2761 acctgagaga gggcaatagt tctatcgctt caaatttttt cttggtttgc agatattcaa
     2821 cattcggtcg tatttctata gtattcggca gatttattac agccaagcat ctcaaaaata
     2881 cggacagcat cctccatctt tttctggcct tccttgactc taccttgctt gctatcaagg
     2941 agaccttctg cccacagata aacaattcgg aaataggtct cattttcctt gtagaaatgc
     3001 tcttcgataa cacgtttaaa ataataggca ttggtaaatt cttcacactc aatactagct
     3061 aaaaagccgt tcaatagtat agtatgaaaa aggtttcgat tgccagacat ttccattaga
     3121 aaatcagatt tacgtaccat ttctcgtaca tatctagtaa aaagagaaac agataaaaat
     3181 ggagaactga ctgaaaataa attgagttca tagattcccc agatctcggt agaaaacaaa
     3241 taatcatgaa ggacttttcc ttcctctgct gttaagtcta ccctttcatc tatgctcttc
     3301 atataagact tgataataat ggcatttaga atatgtttct gtttgttgtg agaatgggca
     3361 tgcttttgat actccctgcg atataagtcc tcaagaggtg ctatattctt tggttccaag
     3421 acatctgtaa tttcttttct caactcagaa tctgtatcat actggaaacc tctcgccaga
     3481 aagaggatct cctccacact ggcagatata ttttccagag caaatagaaa cttttccacc
     3541 gaaagctcac tctgacctgt ttcaaaacgg gacaacatag acggcgaaaa ttgtcctccg
     3601 gttgcttgtc tcagtgagat atttcttgac tctcgtaatt gtctaaagac ttttccaatc
     3661 tgctccatag acttcccctt gattccgtat tttcttcatt ttatcatatt tttcagaaaa
     3721 ttaatcaaaa gcttgccaaa ttgtcagaat tatgagaaaa tagaggatat ttatcacatg
     3781 gagggactgc tatgagagac gatatcaaaa tcaatgaccg tgctttggcc ttgcaagacc
     3841 aaattatcga aaaactagag aaagtttttg atacagatgt ggaattggat gtttacaatc
     3901 taggtctgat ttatgaaatc aatctggatg aaacggggct ctgcaagatt gtcatgacct
     3961 tcaccgatac tgcctgtgat tgcgccgaaa gcctgcctat tgaaattgtg gcaggtctga
     4021 aacaaatcga gggtatcaaa gatatcaagg ttgaagttac ctggtcgcct gcttggaaaa
     4081 tcacacgaat cagtcgctat ggccgtattg cccttggact accacctcgt taagcaggcc
     4141 aatcactttt aaagatgaaa aaagcaagcc gaaaatggct tgctttttga tactcaatga
     4201 aaatcaaagg gcaaactaga aa
//
LOCUS       DAVBDG010000104         4172 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBDG010000104 DAVBDG010000000
VERSION     DAVBDG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.106"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            52..693
                     /locus_tag="VQ526_001993"
     CDS             52..693
                     /locus_tag="VQ526_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2555657.1"
                     /translation="MRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAGA
                     FSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLDAAYIQNGECKTSAQLLT
                     NKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYRI
                     PATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(735..2723)
                     /locus_tag="VQ526_001994"
     CDS             complement(735..2723)
                     /locus_tag="VQ526_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2555658.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2725..3483)
                     /locus_tag="VQ526_001995"
     CDS             complement(2725..3483)
                     /locus_tag="VQ526_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2555659.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3911..4102
                     /locus_tag="VQ526_001996"
     CDS             3911..4102
                     /locus_tag="VQ526_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555660.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1263 a          821 c          827 g         1261 t
ORIGIN      
        1 tcagaaacaa atcacgaaat cgattcaaat tttgcaggtc gtttaaatat cctgcgcgca
       61 ggtgttcttg gtgctaacga tggaattatt tccattgctg gtgtggttat cggggttgcc
      121 agtgccacga ccaatatctg gattatcttt ttatcaggat ttgcggctat cttagctggt
      181 gccttttcaa tggctggtgg agaatatgta tccgtttcaa ctccaaaaga taccgaggaa
      241 gctgccgttt cgcgagaaaa actcttgtta gaccaagata gggaactagc caaaaaatcc
      301 ctcgatgctg cttatatcca aaatggagaa tgcaaaactt ctgcccaact cttgaccaat
      361 aagatctttc ttaaaaatcc actcaaggct ctggtagagg aaaaatatgg gattgagtat
      421 gaagaattta ccaatccttg gcacgctgcc atttctagct tcgttgcctt tttccttaga
      481 agtttgcctc caatgctgtc agtgaccatt tttccaagtg attaccgcat ccctgctact
      541 gtccttattg tcggtgtggc ccttcttctc actggttaca ctagtgccag acttggaaag
      601 gatccgacta gaacagctat gattcggaac cttgctattg gtctcttgac catgggagtt
      661 accttcctgc tcgaacaact tttcagcatt tagaatacaa gaaatacctc gattttgaag
      721 tcgaggtatc ttttttacat ttgcacaatc ttgcgataac ttcttgaagt aatcatgaaa
      781 atcagcacat aggcgatgag gaagatagcg cagatagaca aggtcacaat caacatcata
      841 gtcgtatcca gtacaccaat cacttttaaa atcaggctaa gcatatggta ggcaaaggcg
      901 agatgtatga aggcaaagag caaaggaagg aagaaaacag ttaaaacctg tttgttgatg
      961 gtttgcttga tttgcttttg gtccaaacca actttctgca agataataaa gcgttcacgg
     1021 tcttcgtagc cttcagaaat ttgtttgtag tagatgacca gaacagttcc gaccataaag
     1081 ataatggata ggaaaatacc gataaagaag acaccaccaa agagggcact catctgagaa
     1141 ctagcatctg ctagattgct accataaaca tagctacctt ctgtgtctaa ttgagcatta
     1201 aattgattga ggtagttttc atactcctca gcgaccttga gttgttcttc ttcgctggca
     1261 tctacattca taccaccgta aaactgatta tagatagccg aatctgggaa ttgatccaaa
     1321 aaggcttgta aatcaggaac aacaaggtaa ttgtaatcag tagtcaagat attaaactta
     1381 tttggaacat ggttcacaat gaaatcttta ttaaattctt ctttgacaga aaattgatga
     1441 tcatttagag ttagagcttt ctgtcctttc agtccgtcat ttttggcaaa gagaccgacc
     1501 tcatttcctg atagagacag tttttgacca gtcatatttt cataatcttt ttggtcaaat
     1561 accatgaaaa ctgtttttgg ttggacacgg ttttgtcctt tttcaaaaat agttaacttg
     1621 gttccttctt gattcgcaac agcaaagtaa gtgtaacgaa gcacttcttt ctctttaatc
     1681 ttataacctt tgtcacttgc aaactggctc aagagtttgt ccaaatcttc tttttcaaca
     1741 ttttgccctg aaaccccaaa atcatgagga tttagaactt ttttaaagga ttctgcggaa
     1801 ttgaaaatgc ttgtcgctgc tgacatggtt accaaaacca ttgttgacaa aatagcgata
     1861 gttgctagtc caactgcatt tttcttcata cggaaaatca agttagaaac agatatgaga
     1921 ttattaggtt ggtaatagta tttcttattt ttctttaaga tttgaaggaa aacggtaatc
     1981 cctgcattga acaaaagata agtaccaaag ataaccagta aaacagctat gaagaaggtt
     2041 gttaaggctg taagaggatc ttttaccgta agggcaagat aatatccaat ccctaaactt
     2101 atggaaccaa gaatagtttg gagaggaagg aagcgacctt ttttctctcc gctagctttc
     2161 tcacgtgaga gctggagggc attcatacgg gcgattcgaa gggcattcag gaacatgagg
     2221 cctaggaaaa tcaaaccgaa gacaagaagt actgtaatga caactttcat ctggaaggta
     2281 gcgaccagtt ccaccttcaa tttcatcagt ttgagtagga aagcgaaaat caacttgtca
     2341 aacaaggctc caataccgat tcctgctcca acagttagaa tcccaaatag cactaactcc
     2401 ttaaaggtca tactgattag atggcgcttc tccaagccta acatgccata tatacccagt
     2461 tccttggaac ggtttttcat gacaaaacta ttggcataga ggacgataat ggctgacgca
     2521 agggtaacga caaacatacc aaatccaagt gttgcttgaa tggtggttcc tccacggatt
     2581 tccgcaatct ttggattgaa ggttagggag taaaagagat aggtgatggt gactgccaag
     2641 agaacagcca gtgcaaaggg atagtagagt ttgcggtttt taatcaagtt cgataccgct
     2701 aacttattgg ttaatcgaaa catactaatt cacctcgctt gccatgacag tcaaggtatc
     2761 agagatttct tggaacatct gacgttctgt cttctctcca cggtagattt gattgtaaag
     2821 aatgccgtct ttgataaaga gaacacgctt ggccctgcta gcagctgctg ttgagtgggt
     2881 taccatgagg atggtttgcc cacgctcatt gatttcatta aagacatcaa gtaaggctgc
     2941 agatgacttg gaatcaaggg ctcctgttgg ctcgtccgca aggagaattt caggttctgt
     3001 gatgatggcg cgggctactg ctacacgctg tttctgacca ccagaaatct cgtaagggta
     3061 cttctcttgc aattggttga tacccagatt ttcagctgtc atcaccaatt tcttcatcat
     3121 ctccgttata ggtcttcttg acaagacaag cggaagcaag atattgtcct taacagacag
     3181 agtatctagc aagttaaagt cttggaagac aaatcctagc ttttcacgcc ggaaactaga
     3241 agcctgtgaa tttttaatag ttgctgtatc agttccgttt aggtaaacct gcccacgact
     3301 tggtttatcc agcatagcta ggatattgag cagagtggat ttaccagaac cagactcacc
     3361 catgatagca acgtagtcac ccttttctac ggtgaagtga atatccttga gggcctctac
     3421 ttggttgccc tgaaaacgtg ttttataaat tttttgaacg tgttttacat ctaaaagtgt
     3481 catgagaatc tcctttctta atatcccatc aaatgaaatg tagcttgcat catagtgcat
     3541 cccagctgaa cgcgctcaat ttctttgtga cttggttttc tagttttctt atgtaagatt
     3601 tgtttcatga tgttactcct ttgttcttta tgagtctagt ttacatcaaa aaaagaccgc
     3661 ttgccataac ctaaccttac aaaagagact ttaatcttac atttttgtaa gattgggtga
     3721 aatataagca aaattactta gaaaatgatt tgatttccta tttttaaagt tgtaaaatat
     3781 aagtgtgaaa aggcttactt tgaaaaggag gaagcgggaa ctaacatttt agtaaggtta
     3841 cctagataac ataccattca atttaacact tgcaagtcgt tcaacctata aggaaagaag
     3901 gtgcgcttta atgaaacaca aagaacatat actcatcgga cttctctatc tgctctctcc
     3961 attcatcggt cagctcttag ttgaacacac acactttatc ggtacagaat ttaccggcac
     4021 tgcttatgtt atttgctggt tatcagttgt tatcagcatc caccattttt ccaaaaatgt
     4081 attgtctcag cagcaaaaat agattaaaaa actgagttta aattactgct tttataataa
     4141 aatgaaataa gaacaggaca aatcgatcag ga
//
LOCUS       DAVBDG010000105         4002 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBDG010000105 DAVBDG010000000
VERSION     DAVBDG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.107"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..410)
                     /locus_tag="VQ526_001997"
     CDS             complement(<1..410)
                     /locus_tag="VQ526_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2555661.1"
                     /translation="MLLVLVSLVHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSGLVVQEFLGDYSGYV"
     gene            complement(486..1336)
                     /gene="rfbD"
                     /locus_tag="VQ526_001998"
                     /pseudo
     CDS             complement(486..1336)
                     /gene="rfbD"
                     /locus_tag="VQ526_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1402..2451)
                     /gene="rfbB"
                     /locus_tag="VQ526_001999"
     CDS             complement(1402..2451)
                     /gene="rfbB"
                     /locus_tag="VQ526_001999"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2555662.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPDHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2461..3057)
                     /locus_tag="VQ526_002000"
     CDS             complement(2461..3057)
                     /locus_tag="VQ526_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2555663.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3058..3915)
                     /gene="rfbA"
                     /locus_tag="VQ526_002001"
     CDS             complement(3058..3915)
                     /gene="rfbA"
                     /locus_tag="VQ526_002001"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2555664.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1119 a          924 c          725 g         1234 t
ORIGIN      
        1 acatagccag aataatctcc taggaattct tgtactacta aaccactccg acgctgatca
       61 tggtggtaca gcgtgattgc ttgattctca gctttcccag acaaaaaagt ccaatagtag
      121 gtcagatggc tatcactctc tagaacccga taagaggttt catccgcatg aagaagaggt
      181 tgttctaaca acttttctcg taaaaggtta taaagggact ctaaatagta ttgacttgcc
      241 ttgatatgcc aattagagat ttccttacgt gtgattggta aacccatcct agcccaatct
      301 tcttcttggc gataattggg taccttcaga ttaaacttct gatggatggt gtgagcgata
      361 atagaaactg agccaaagct atgcaccaaa gagaccagca ccaacaacaa gataattgta
      421 catcttaagc tcctttggag ttcattatct gcctctttta ttagactaga aaatcattct
      481 actacttatc tcacttcttg tttgtaaaat tcttgcaatg catcttgcca agttggaata
      541 acaaatccag tagctttggc tttggccagg ctcatcgttg agtttagcgg acgttgagct
      601 ttggctggaa attgactgga tctactggct tgacttcgac atctgtatct ttcaaaattt
      661 caactgcaaa atcataccat gttgtgtctt ctgtcgcatc atttgacaaa tgataataac
      721 caaattcctt acgattttca gctaggtaga tcatgaattc agccaaggta cgagtccaag
      781 tcggacgacc gtactggtca tttacaactg ttaaagtctt atgagttttc gcaagatttt
      841 gcatggtaaa aacgaagttt ttgccataat ttccaaatac ccaggcagta cggataatat
      901 agaaattaga cacatgcttc tcaactaact cttcccccat acgcttagtg cgtccatatt
      961 ctgtctgtgg atctggtcgg tcatcaactt cccactcttg tccaactggt ttcttaccgt
     1021 caaagaccta gtccgtagaa atataaacta gagttgcacc atgcttttca gatgcttttg
     1081 cgacattttt tgtccccatc acattgatgg cgaagtccaa ctcttttcct tcatcctctg
     1141 ctgcatcaac agcggtgtag gctgcacaat ggtagactaa agtcggtttc acctcttcaa
     1201 aaactttctc aaccatttct tcattggtaa tgtccatctc agccacatct actgccacgt
     1261 attcttcatt acgttcatcc aataaatagc gaagttccgt tcctaattgg ccatttgccc
     1321 ctgtaattaa aatcatctct agacctttct aatatgtaac tcttcccaat atagcaataa
     1381 aaagcagcga tttcctgctt tttatactgt aataatctcc tgagtcttag cataattggc
     1441 ttcaacagct tctttttctg ctttccacca ttcttggtta tctgtatacc acttgattgt
     1501 tgccttgagc ccagcttcaa agttggtaaa ttcaggtttc caccccaact catcacggag
     1561 cttgctggca tcaatcgcat agcgaaggtc atgtcctgca cggtcagtca catgatcata
     1621 ggcatccgta gcttgtccca tttccttaag gataagttcc aaaacttcct tattgttctt
     1681 ctcaccatca gccccaatca agtaggtttc accgatttgc ccttttgtca agattgtcca
     1741 aactcctgaa gaatggtcat tggtatgaat ccagtcacga acgttcttac cttcaccgta
     1801 aagttttggc ttaattccac ttaggatgtt agtaatctga cgtgggatga atttttcgat
     1861 atgttgataa ggaccgtagt tatttgaaca gttggaaatc gttgccttga ctccaaaaga
     1921 acgcacccag gctttgacaa tcaagtctga tgcggccttg gttgatgagt acggagagct
     1981 tgggttgtat tttgtttcag cagtaaattt ctcacctggt ccttcaccat gatctggcaa
     2041 atcttctcgt aaagggagat ccccataaac ttcatctgtc gatacatggt ggaagcgaat
     2101 atcatactta cgagcagctt ctaaaagagt atagattcca atgaagttag tatgaataaa
     2161 tggcgatgga tcattgagcg aattatcatt gtggctttcc gctgcataat gaacgatagc
     2221 atctgcttga gcaaccaact tgtctaccaa ctccgcatca gcaatgtcac caacaactaa
     2281 ctcaacacga ttgcctaaaa tttcctcaat attcgcgcgg tttccagcat aagtcaactt
     2341 atctaggact gtcacgtgaa catctggaaa gttctcgtaa acataatgga caaagttaga
     2401 accgataaag ccagctccac ctgtcacgat aatatttttg tattcagtca tattctttcc
     2461 ttattccaaa tcttcttttt tcaaaggctt cacgtcctta agtaggggat gatgtttgtc
     2521 tgcttcagat acgtctgctt cttccagatt ttcccattca ataccaaggc ttggatcagc
     2581 gtagttcaca aaggcatact tgggtttgag ttcaagagcc cagtaatcat tgaccagata
     2641 gctatatgac actgtatctg ataaaacttg gaagccatta gctacgcctc gaggaacaaa
     2701 gattcccttg cttgcatcaa ttactgtctg ataggtattc ccaaaggttt caccctcgcg
     2761 tagatcaacc caagaaccca gaactttccc tccatctgct acagagatgt acttatccca
     2821 aggctctgcg tggaggcctc gaaggacatt tttacgtgag aaggatacat tgttttgcaa
     2881 ttttccttct gcaaagaaag actctggaaa tccaagtgga agcacttttt ccttttggaa
     2941 attttcttta aaccagccac gattatctcc atgaacgggg atatcaaact ccaacatgcc
     3001 tggaatagct tcaaccttgc gtgccgcaag cgtcttaccg aaaaaattat ctgtcatcta
     3061 tgcttctcca atcaaacgga gcagatactg tccgtattca tttttcttaa gtggttgggc
     3121 taaggccaat acatcttctc gactgatata gcccatacgg taagcaattt cttctaagtt
     3181 tgctacctga acattttgca tccgttgcac tgtttcgatg tactgtgaag cctctagtaa
     3241 actttcatga gttccagtat tcagccaagc aaagccacgt cccataagct caacagacaa
     3301 atcaccacga tctaggtaag ccttgtttac atctgtaatt tccagttcac cacgagggct
     3361 tggtttaata ctcttggcaa tttcgacaac atcgttatca tagaaataca gtcctgtcac
     3421 tgcatagttg gagcgaggat actccggttt ttcttctatg gaaatagcat tcatgtctgt
     3481 atcaaactca accacaccaa aacgctctgg atccttcact tggtagccaa aaacagtcgc
     3541 acctttctct ttcttggctg ctttttgaag cattgtgctc agaccaggac catggtagat
     3601 attgtcgcct aaaatcaagg caacgctatc gtcagaaata aactcttccc caattgccaa
     3661 accatctgta cttggttgct ctgcataaga aagtttgatc ccaaactcag atccgtcttg
     3721 aagaagctct tgaaatcgat gtaaatcctg tggagtggaa ataatcaaaa tatccctaat
     3781 cccagccaac atcaatgttg aaagtgggta gtaaatcatc ggtttatcat aaaccggcat
     3841 aagttgtttt gacgcagcgc gagtcaaagg atataaacgt gtccccgaac cacctgctag
     3901 aataatacct ttcatagtag ggtacctttc tttgttattt tagagtcaat tactattaat
     3961 tgtgtactgc tctaaaaaga aaacaatcat cccatgtttt at
//
LOCUS       DAVBDG010000106         3984 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBDG010000106 DAVBDG010000000
VERSION     DAVBDG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.108"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(382..915)
                     /locus_tag="VQ526_002002"
     CDS             complement(382..915)
                     /locus_tag="VQ526_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2555665.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1004..1780)
                     /gene="recX"
                     /locus_tag="VQ526_002003"
     CDS             complement(1004..1780)
                     /gene="recX"
                     /locus_tag="VQ526_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2555666.1"
                     /translation="MKITKLEKKKRLYLMELDGQQTSYITEDTIVRFMLSRDKVISKE
                     ELTEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPSRALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYSKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            complement(1790..2566)
                     /locus_tag="VQ526_002004"
     CDS             complement(1790..2566)
                     /locus_tag="VQ526_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HET2555667.1"
                     /translation="MNSKTPRFSILDFPEQLRSYIKGANLSDSSSHSSATVLYIDSGY
                     YLKIDQKGRLAQETKIASWFETEGLGTPVITYLSANKDFLLTKEAIGSNALAFLNQPE
                     RLCQTLAQALNKLHSLKPQSFPSENHLKRYKEKALKNYQKGTFYNKTLLPQFHIHSRE
                     EAYQLIQEKGYILKADAFIHGDACLPNFILKDASHFSCFIDLGLAGFSDRHIDLFWAI
                     WSLHYNLQDATYAELFLDYYGREYVNMDKLRLVAAFEAFR"
     gene            2623..3978
                     /gene="rlmD"
                     /locus_tag="VQ526_002005"
     CDS             2623..3978
                     /gene="rlmD"
                     /locus_tag="VQ526_002005"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2555668.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKENHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1266 a          698 c          745 g         1275 t
ORIGIN      
        1 atttcatttt actatactat tacgaaatta gatatataaa tgaaaacttg cctcaaggta
       61 ggtttttttg ctagtagaaa agcgatagac agaaattgtt ggactcttaa aaaaatgaag
      121 ttttgctcaa aagagcttct taaattaaaa cgagcaatta tcagttcaaa tctgtttcaa
      181 aagaagaacg gataaattaa tcgatgcttt gtttttgagt taggaagtta agcatgccaa
      241 actgagtcgt aggaataaat ctctttctag taatgtgttg taactctgct ataatggatt
      301 tattcctttt tgtgtttaca caatttattt tatactacca aaaaaacaag gtcaggattt
      361 tgttcctgac ctttgacaac tttaccgatt ctttagttct acatagcgct tgtaccaaat
      421 gtttacatag gcttctgaga aaggaccacg tccattgtta atccaatcaa caagaatttt
      481 gacatgttct tttaaaatat agtccaagtc atcagaataa ttcattttgc gtttgtgacg
      541 ctcgtactct tcaacgtcca agagacgttt ttccccatct gtaaaaattt taacatccaa
      601 atcgtaatca atatacttca gtgcttcttc atccagatag taggggctag ccatattgca
      661 atagtaagaa gttccattat cacgaatcat ggcaatgata ttaaaccaat atttcttgtg
      721 aaagtaaaca atagccggtt ctcgagtgac ccaacgacga ccatcacttt cggtaacaag
      781 tgtatgatcg ttgacaccaa taatggcgtt ttctgttgtt tttagtacca tggtgtcccg
      841 ccaagttcgg tggagactcc catcatgctt ataactttga attgtaataa agtcgccttc
      901 ttttggaagc ttcataacta accaactttc tacaatttat aagtttatca tttactattg
      961 taccataaaa ttacctaaaa tctgtgaatt tcacttggaa atattaaaga tattctctaa
     1021 gagcgcttgc tatatccgaa aaatcgtagc cctttcgtgc taaaacttga gttaaacgct
     1081 gcttcagttc gtatccttca tactttcgag catacttact atattgttta tcaagttcct
     1141 tgaagatgag ttcttgagtc gtttcttggt caacttggct atctaagtcg tcaaaggcaa
     1201 ttttagcatc agaataagag aagcccttgt tagtcaagtt ctggataatc ttatcttgca
     1261 gggcacgaga tggaagtttt ccctcatatt ttttcaatag tttattagct acacgttgag
     1321 caacttcaga aaaatcaaat tctttcaagt tctcttctat agtagatttt gaaatccctt
     1381 tttgagctag tttctgagtc agcacataag gacccttgtc tcctgaaagt tgattggtat
     1441 tgatgatagc ataagcgtac tgaccatcat taatccactt atcttcttta agattagcaa
     1501 taacttgaga aacgatgttt ttatcaatat cgtatttttt cagatattct ctgacttctt
     1561 tttcagtgcg tgctttaaag gataagtggt agagagcgag attcttacca taagaaaact
     1621 gggcaaaatc ctgaatctcg gtcagttcct ctttactaat gaccttatct cgagacagca
     1681 taaaacggac aatggtatct tccgtgatat aagaggtttg ctgtccatct agttccatca
     1741 agtagagacg ttttttcttt tcaagttttg tgattttcat aatttccttt catctaaatg
     1801 cttcgaaagc tgcaacaagt cgtagtttat ccatatttac atattctcta ccgtaatagt
     1861 cgagaaataa ttcagcatac gtagcatctt gtaggttgta atgaagggac caaattgccc
     1921 aaaagaggtc gatatgtcgg tcactaaaac cagccaatcc caagtcaata aagcaagaaa
     1981 aatgagaggc atccttcaag atgaaatttg gcaggcaggc atctccatga ataaaagcat
     2041 cagctttgag aatgtaacct ttctcttgta tcagttggta agcttcttca cgactgtgaa
     2101 tgtgaaattg aggcagaaga gtcttgttat aaaaagtccc cttctgatag ttttttaagg
     2161 ctttttcttt ataacgcttc agatgatttt ccgacggaaa agattgtggt ttaagactgt
     2221 gaagtttgtt taatgcttga gccagggttt gacagagtct ttcgggttga tttaaaaaag
     2281 cgagagcgtt gctgccaatg gcttctttag tcagaaggaa gtccttattt gctgataaat
     2341 aggtaatcac tggagttccc aatccttcag tttcgaacca acttgcaatc ttagtttctt
     2401 gagctaatct gcctttttga tctattttta aatagtaacc tgaatctata tataggacag
     2461 ttgcacttga atgagaagaa ctatcagaga gattagctcc cttgatatag cttcgtaatt
     2521 gttcagggaa atctagtata ctaaagcgag gtgtttttga attcatgctt ttattgtaac
     2581 atattcttaa atatcttaga aactccatgg tataatagac atatgaatct gaaagtgaaa
     2641 caaaaaatac cattaaaaat caagcgcatg ggaattaacg gtgagggaat cggcttttac
     2701 caaaaaacat tagtctttgt accaggagct ctcaaaggcg aagatatcta ttgtcagatt
     2761 acttctatta gacgcaactt tgttgaagca aaattactga aggtcaacaa gaagtctaaa
     2821 tttcgaattg tgccatcttg tactatttat aatgaatgcg gaggctgcca aatcatgcac
     2881 ctgcattatg ataagcagct ggagttcaag acggacttac ttcatcaagc gctgaaaaaa
     2941 tttgctcctg caggatatga aaattatgaa attcgtccaa ctattggaat gcaggaacca
     3001 aaatattaca gagctaagtt acaatttcag actcgaaaat ttaaaaatca ggtcaaggcg
     3061 ggcttatatg cacaaaactc tcactattta gtagagttga aagactgcct ggtacaagat
     3121 aaggaaaccc aagtgattgc taatcgctta gcagaattac ttacttatca ccagattcca
     3181 atcacggatg agagaaaagt tctaggtgtc cgtactatta tggtccgacg cgcgagaaag
     3241 accggacagg ttcagattat tattgttaca aaccgccagc ttaatttaac tcaattggta
     3301 aaagagttgg ttaaagattt cccagaagtt gtgacagtag ctgttaatac aaatacagct
     3361 aaaaccagtg agatatatgg tgaaaagaca gagattatct gggggcaaga gagtattcaa
     3421 gaaggtgtac tcaattatga attttcacta tcccctcgag ctttttatca actaaatcct
     3481 gagcaaacag aagtcctcta tagcgaagca gtaaaagcgc tggatgttga taaagaaaac
     3541 catttgattg acgcttattg tggagttgga acgattggat ttgcctttgc aaagaaagta
     3601 aaaacactca gaggtatgga tattattcca gaagctattg aagatgccaa gcgaaatgct
     3661 aaaagaatgg gatttgacaa tactcattat gaagctggaa cggcagaaga gattattcct
     3721 cgttggtaca aggaaggcta ccgagcagat gctttgattg ttgacccacc acgtacaggt
     3781 ctggatgata agttattaga tactattctt acttatgtac cagaaaaaat ggtttatatt
     3841 tcttgtaatg tttcgacctt ggctcgtgat ttggtacgct tagtagaagt ctatgatctt
     3901 cattatatcc agtcggtcga tatgttccca catacagctc gaactgaagc tgttgtaaaa
     3961 ttaataaaaa aagtttaaaa aagg
//
LOCUS       DAVBDG010000107         3523 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBDG010000107 DAVBDG010000000
VERSION     DAVBDG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.109"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..236)
                     /locus_tag="VQ526_002006"
     CDS             complement(<1..236)
                     /locus_tag="VQ526_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HET2555669.1"
                     /translation="MVVTKYFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFTNNDKLYESRNDRQEKHQQT"
     gene            complement(223..521)
                     /locus_tag="VQ526_002007"
                     /pseudo
     CDS             complement(223..521)
                     /locus_tag="VQ526_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480038.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
     gene            complement(597..956)
                     /locus_tag="VQ526_002008"
     CDS             complement(597..956)
                     /locus_tag="VQ526_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555670.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIVE
                     VNQAQPLSREFCEKYMG"
     gene            complement(1775..2932)
                     /locus_tag="VQ526_002009"
     CDS             complement(1775..2932)
                     /locus_tag="VQ526_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555671.1"
                     /translation="MRGFNNKIKSVYQELTNSKEKFGSFHKTLIHLHTPVSYDYKLFS
                     NWTATKYRKITEDELYDIFFENKKIKVDKTIFFSNFDKVVFSSSKEYISFLMLAEAII
                     KNGIEIVVVTDHNTTKGIKKLQMAVSIIMKNYPIYDIHPHILHGVEISAADKLHIVCI
                     YDYEQESWVNQWLSENIISKKDGSYQHSLTIMKDFNNQKIVNYIAHFNSYDILKKGSH
                     LSGAYKRKIFSKENTRFLEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYS
                     DYSKDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQV
                     VIMESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            complement(2960..>3523)
                     /locus_tag="VQ526_002010"
     CDS             complement(2960..>3523)
                     /locus_tag="VQ526_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET2555672.1"
                     /translation="SFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLVTKLSSLRY
                     NLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPKLSYLSTLIRYEELYAINPNQ
                     ARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLHE
                     LLFGEWSQVEKEMLRVGEKRLNELLEK"
BASE COUNT         1043 a          693 c          506 g         1281 t
ORIGIN      
        1 gtttgttggt gtttttcttg tcggtcattt ctggactcgt acaacttgtc gttattagta
       61 aagttaacat tgtacatttc taccatttct gcatggctag gaaaatctaa gtaattactc
      121 atgccaaaat cagatactaa ttttagatta tctgttttat ctggattgag gatatattta
      181 atcaaacgcc tacgatattt ttttccgtgc gtcgcaaagt atttagtaac taccatgaaa
      241 ctccttcagt cttttcacct ccacttgaaa ttctttgtcc acttccttaa ttaactctcc
      301 gactccttta ctcaattctt gtaattggtc ctgagaaatc agatggcttt gattaatcgc
      361 acgcgcaatt tgattgatgt tatttccaat cctcctcaat tcaaacacta actgttcata
      421 agtatcagtg ttaatcgtga caaatgacat attgggatta agcaaaactt ttctggcgta
      481 gactgagaaa ttttgacagt gactcttagt aatacgatca tttaattaat tcaattctga
      541 atcagttaga aatatttttt tagattagta cgataacgat aaaccatagc agtctcctat
      601 cccatatatt tttcacagaa ttcacgactg agtggctgag cttgattgac ttctacaatt
      661 aattcttgaa cacaagtcaa taagattgag acgtgctctt gagtcacttg atgattctct
      721 cttgcgataa tcaaaaccaa atgcacatct cgactgatct gttcaaactt ttgagactgc
      781 caaagggaaa accattcttt ggcagctaac tgtttgtcat cagtcatcaa aattttatcc
      841 ctgacgaatg gtgaaaagtg acgataatta tcaacctcca tcattttttc aatctgtttt
      901 agctcatctg cggttagatt tacctctttt cgaatgtcac ggtactgttc agacatctct
      961 tttcctcact ttcatccttt ctcgggtaaa ccccgacccc gtgtgtcata cgggaaattt
     1021 cttaaagtcc ttgttcggtc tttgcgagct caggcattgt ggtcacaata cccaaaaaat
     1081 catatcgcca gctaaccaaa accttccagt tttgacagct aaccataaga taacttggtg
     1141 gctacgcccc caaactccca ggtaaaattc ttagttggat ttttaaaagt tgagaaaagt
     1201 atagcagaag aaagttgggg aagttatcac aaatcaagta gggactaatc tctaacacca
     1261 ctgcatgtac cgtcctatat ttatgatcag ctcccatcgt ttacattttt aggtcactgt
     1321 cttttcatac caggaattag aattgacaaa tttaattaga gaggtattca tttaattgtt
     1381 cacttcttcg ctccatttcc attacagaaa tttcttcaat actatgtgtt cagctgactc
     1441 ccatgattcg ttgtactacg tcctaaacgc tcacggaata agccgtacat ctttcctagt
     1501 ttactctcgt aatttacgta tataggttac gacacctatt tggattttag agttcctcat
     1561 ccgctatata tggcttcaag tcacgattct gtttgtagaa ccttgatttc gctaattcct
     1621 tcctatcccc cctacctcac gatattcagg cttaggagtc gctattggtt tcatctgcag
     1681 caaatatcca cagaggactt ggacctcaga tgtacactat gcctgtcgaa caaagaaaaa
     1741 agtaggctaa atttctaacc tactttttct gactctactc tgataaagcc gtctcatata
     1801 tagacatttt atgcttgaag gaatcttttc ctccctctaa ttgattgatg atatgatttt
     1861 ttatatattt ttcactaaca tatccctgtg attcaatcca tccgttaact ccatctgact
     1921 ccataataac aacttgatct gtcatagaat ttgttacaat tgtagcatta tgtgttgcta
     1981 aaataatttg acgttgagct ttcacatctc taaactgctg aactaaatgc ctgtaaatat
     2041 aacgattgtc tagattgtct tcaggctgat caataatcaa tggtctgaag tctttagaat
     2101 agtcactata tgctaataaa aaatcaagca tggctacaac tttttgtccc aaacttaata
     2161 caccaacttc tttatagaga atatcaagtt gttgcgaaga ttctttcgag ttaatattaa
     2221 actccaaaaa tcgtgtattt tctttagaaa aaatttttcg tttatatgca cctgataagt
     2281 gagaaccttt tttcaaaatg tcataactat tgaaatgagc aatatagtta actatttttt
     2341 gattattgaa atccttcatt atagtcagtg aatgttgata acttccatct ttcttactta
     2401 taatattttc acttaaccat tgattaaccc atgattcttg ttcataatca tatatacata
     2461 caatatgcaa tttatctgct gcactaattt ctactccatg taaaatatga ggatgtatat
     2521 cataaatcgg ataatttttc attatgattg agactgccat ttgtaacttt ttaatacctt
     2581 tggtagtatt atgatcagtt actacaacta tttctattcc attttttatg attgcctctg
     2641 ctaacataag aaaactaata tattcttttg aactagaaaa aacaacctta tcaaaattac
     2701 taaaaaaaat tgtcttatca acttttattt tcttattttc aaaaaatata tcatatagtt
     2761 catcttcagt aatttttcta tatttcgttg cagtccaatt agaaaatagc ttgtaatcat
     2821 aagaaacagg tgtatgcaaa tgaattaaag tcttgtgaaa gctaccgaat ttctctttgg
     2881 aatttgttag ttcttgataa acagacttta tcttgttatt aaaccctctc atttctactc
     2941 tcctaaaaat atcaattgtt tatttttcaa gtaattcatt aagtctcttt tcccctaccc
     3001 tcaacatctc cttttctacc tgactccact ctccaaacaa taactcatga agaacatctg
     3061 ctgctgaagt tgtattttct tttgaatcat atacacaact tctatctcgt tggtaaattt
     3121 gaattctttc aaaaatagct agttcttcca attgtcgtgt attatcaact agatgattta
     3181 caatgaaatc atgatgttct tttggtgttg cgcgtgcttg atttggattg atagcgtaca
     3241 gttcttcgta tcgaataaga gtgctcagat aggacagctt aggctttgtc gcaatcaagg
     3301 ctaattgtac ttcatatccc ttatttttca agagttgtgc cgttttcttt ggaacatcaa
     3361 ctgttcgtaa agttccctct atcaaaagat tgtatctcaa actactcaat tttgttacta
     3421 aagactctac catttttcct gcaaaatctt tggtatattc tacactgtct ttgccatatt
     3481 cttgctgcag ttctaaatag tgtggatgct gagaacgaaa act
//
LOCUS       DAVBDG010000108         3395 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBDG010000108 DAVBDG010000000
VERSION     DAVBDG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.110"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..323
                     /locus_tag="VQ526_002011"
     CDS             <1..323
                     /locus_tag="VQ526_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HET2555673.1"
                     /translation="DGGGVGRNWWNSRGSFATVLDVGLAIYSGGATIYSAYAIKKAIS
                     ANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTGFSLGGAIAYGADWADGSLDG
                     YIFA"
     gene            697..1755
                     /locus_tag="VQ526_002012"
     CDS             697..1755
                     /locus_tag="VQ526_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555674.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIGTVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILIFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKR"
     gene            2070..3046
                     /locus_tag="VQ526_002013"
                     /pseudo
     CDS             2070..3046
                     /locus_tag="VQ526_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1115 a          439 c          604 g         1237 t
ORIGIN      
        1 ttgatggggg tggtgttggt cgtaactggt ggaatagtag aggtagtttt gcaacagttt
       61 tggatgtagg tttggccatc tatagtggtg gtgcaacaat ttattctgct tatgcgataa
      121 aaaaagctat ctcagctaat agaggggcta ttacgagaac attacgtagt ttaataatta
      181 aacatgtagg tagtgcagct ggccatttag tcaatactgc actaaacgtt gcactaactg
      241 ttactggatt ttcactaggt ggagcaatcg catatggggc tgattgggct gacggtagct
      301 tagatggtta tatttttgct taaaatgaaa catttttttg caggaattgg tgaaattcat
      361 tttttgttat ttttattata tctaagcatg tctgctagtc caattattgg actagtgatt
      421 ggatatacta aaataaatct aataaattct attattatag ctcttggtat gatattggca
      481 ttaattttgg tattatcaag agtgtataga aaatattttt atagagacga tgatgactaa
      541 agtttttatc aataatttgg gctccttgtc aactgtagtg ggttgaagaa aagctaacgt
      601 ctggggaagg ggttaaagct ttgctttatc aaatattgac ttacagagtt gctctattgt
      661 attgatgtat tcttagtaaa taatatggtg ttttgaatga aagatgatca gaaatattta
      721 ttagcgggac tttattcgct tctagttgca atattttatt ttccattgat tgagtccaag
      781 gggatttttg taagtatatt gatggcggtt ttacttttat atttaatata cttcattgga
      841 acagtcattc atattgtgat aattaagttt ataagaaaaa aatcttttaa gtatttggtt
      901 ctatatccat ttacttatga tgggagctgg agatttcaac ctataaactt actctacttt
      961 ccagaaatgg tgagagatgt cattccgatt aatttagttc aagaatattg ccaaggacaa
     1021 ccttacgggc tgttaaaaaa gatgctgaaa agaattcgat tgtcacgaga gatagcttta
     1081 ctcctggcta ccattatcgt ctattttttt acgcatagaa ttctacctct ttcagtattt
     1141 acgttcatgt tttcttacat attgatattt gcacaatctt atttgggtgg taatactgct
     1201 tggattggaa atagaagatt aataatagat gatgaatttg aaaaaatctt gctttcaaaa
     1261 agttatataa aagaaatatc atctgcacga tactcggaat acttgacatg tgaatacaaa
     1321 aaccttacac caatcatatt gttagctatt tttgaaaatt tacttgactc ctacttgctt
     1381 cagaatcaga gtgaggttga cttagatatt ttttataaag ttttaccttt gctttataaa
     1441 gaaaaatata ctatgggatt caattacttt gtaagtttaa attatttact atataaggtt
     1501 ggatttttgg gaatcattta cgataatgaa gcattaggag atttgtcaaa acaatattta
     1561 aacagaaata tttcggaatt acaagatggt tctttcgagg gcggtataca agatgctgtt
     1621 gcctctaaac aaatagtagt tataaatgaa tttatagctt gtttaaattc cagatgcatg
     1681 ccatcccaat atgatagatt tttttataaa gatagacctt atattttttc cagaaagagt
     1741 cctattaaga ggtgaaaatt tgttacattt ggattaacaa gtgtcctagg gattagaaat
     1801 aactctagta tagctagata tttttgagta actagaaata tcattaggct acccatatga
     1861 ttgccagaaa agatggctga tgaatcttat ttatactgtt gcagtaggcg ttgtctagga
     1921 taaataagct atcttgatgg gggagattgg aataatttta gtagaaacat taaaggatta
     1981 tgggcaagta gcacaggatt aggtgatgca atgtgctaag ttggcttggt gttagcagta
     2041 gtattattta acaatggaaa gtagactaaa tgaaaaataa acgttatttt tttgacacaa
     2101 ttttaatgat tttactactt ataagcacga tattttgtgt cagcccagta tttataaaat
     2161 tggacatctt aggaactccc tctcatgcca tcttaacctt tgtattggcg ataccgttat
     2221 tctatatttt gtctcaatgc ttacatactt tgctattact agtttcctca atcttttgta
     2281 aattaagacc aatttatttt tattttatat ttgtgatcat tataggagct aggaaatact
     2341 atcgaattct ctttcaccaa ctaatgggat ttagtccagg aatagcagtg ttttataagg
     2401 agagtcaaac aactaagaat ctgtttaaat tttactactt cttgtacttt acaacactaa
     2461 tttcatacta ttttttcttt acttttgtgt atgacaaacc gttgcttttg cctcttattc
     2521 cattttctat tatcatagca ttagtacaaa aactttatag aattgagaat cagcaattat
     2581 ttctgttaaa atcaaaggta ctaacaatac tggaatctaa gaagaattgt gagtttaatc
     2641 ttcaggatta tcatgaaatt tggaaattgc aatcaaaatc tgagttgcca tgcgtggctt
     2701 tatcttatat cagtttgatc aagccatatc tttcagagag cgtgcgtgaa caaattgatt
     2761 tattagaagt taagcgattt aaaaaaataa atcatccaat atcactttat ggtatgttgg
     2821 atgttataaa attaaatcta tatttacggc attataatga aaaaataaat atgaatcaat
     2881 gttaaagaaa attttagagg taagaccaga ctttgtttta atcgagcaga atattgatga
     2941 tagtttaaat agctcgcaac cattatcttt atctttagct atttcagaaa tccaactttt
     3001 gcttgaagtg tatataggga tcaaacatgt ttcaattagg agataaaact agaataacat
     3061 ttgctattat aggggcaagt tgcatattag tcagtatgta tcttatacct ctcattaaca
     3121 cggataataa attttgggaa atacttttat ttataatagg tgtattgcta attggaattg
     3181 cgttactaag tcaaaaaaaa taaattttga aatagtatta gtgagttcgc aatcatttat
     3241 tgctagtggt ttagaactgt taagtttcaa gcaataattg gttttaaacc ttaggtaacc
     3301 tataaaaaca aataaggtag agaaaggata tcttatttag gtaactaagt actataaaca
     3361 cagtcgacaa tcacttgtta ggaaaccatc ctaac
//
LOCUS       DAVBDG010000109         3391 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBDG010000109 DAVBDG010000000
VERSION     DAVBDG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.111"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..463)
                     /locus_tag="VQ526_002014"
     CDS             complement(<1..463)
                     /locus_tag="VQ526_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555675.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
     gene            complement(453..650)
                     /locus_tag="VQ526_002015"
     CDS             complement(453..650)
                     /locus_tag="VQ526_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555676.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(654..1238)
                     /locus_tag="VQ526_002016"
     CDS             complement(654..1238)
                     /locus_tag="VQ526_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555677.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKKFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGDKANMDVVLVFSKLSDGEKKQLLQARVPFVDFKG
                     NLFFPTLGLVLNANDTEVPKELIPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(1636..2061)
                     /locus_tag="VQ526_002017"
     CDS             complement(1636..2061)
                     /locus_tag="VQ526_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555678.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2190..2549)
                     /locus_tag="VQ526_002018"
     CDS             complement(2190..2549)
                     /locus_tag="VQ526_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555679.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            complement(2595..2801)
                     /locus_tag="VQ526_002019"
     CDS             complement(2595..2801)
                     /locus_tag="VQ526_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555680.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLER"
     gene            complement(2788..2949)
                     /locus_tag="VQ526_002020"
     CDS             complement(2788..2949)
                     /locus_tag="VQ526_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2555681.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT         1079 a          689 c          425 g         1198 t
ORIGIN      
        1 gaccacaatt actcaatacc gaatacccct gaatggtttc tttttcatgc accaatatat
       61 tataataatc ttcatccaat aataaggctg ataaactagc atcttggtca aaatgtaagg
      121 gaatttctcg accgtccttc tttaatggat attctggtaa gatactaaat agttcaatca
      181 tagaaggaaa ctcagaatta gttgttgtaa atcgaaaaag ctgcgctttc tcatctttct
      241 gacttccttg atactctccc aattctaaaa aatgattcaa ggtagtataa aattccttat
      301 tttttacttc atcaatgatg accatatcat aatcttttgt tgtgcgactt ttaaatcctt
      361 gcgaatccaa tacgatagag gtagcagttc ccccaatcag aacataatag ttctgaaaat
      421 ccgcaaacat ttcttgaaaa ataactttcg tattagctgg catcatcttc tcccagatac
      481 tgtaatatca tattttctag tgcttcactc tcttcctcta tacgtgggtc atcatcatct
      541 tttaaggtca aataaagaga aatcggatct acaaattgtt tatcatgatt atttttaaaa
      601 tcattccaaa actcagatac aaaaggacga tatttccata tctctagcat ctttcatttt
      661 aaaacatgct gagaaagtgg caaggataac tgattgaatt ttctctgcca tatgacatag
      721 ctagtatttt catccgtttc agctaaaaaa gttgaatgcg acaaagcata agcaccacca
      781 tatagaaggt tagaaacaga ttttatctgc tttatatcgc catctggcaa taaaaaggca
      841 atccacgtta attgttcgct aggtattaat tccttaggga cttcagtatc attcgcattc
      901 agtactagtc ccaatgtagg gaagaagagg tttcccttaa agtctacaaa cggaactcta
      961 gcttgaagta attgcttttt ttcaccgtct gataacttcg aaaacaccaa aacaacatcc
     1021 atattggctt tatcacccat agtgcgagcc tgagtaacaa atgaactcaa actccccctt
     1081 ctcttttctt taatcaataa aaatgattcc ttatgaaaag tcatttgagt atactcaaac
     1141 ttttttacaa aactgattgg taaagtcaaa gtcagttcct caatactgac atcaacataa
     1201 aaggattgaa aaattgacat tatctttttg acattcatgt tttttcctta ttctatcatt
     1261 tttattatat tttatcattc ggtataataa aatataagtt ttttgataaa ataaccaaaa
     1321 actactcgct atcccctctc acattaccct acatatcgtt tgacatgcga ctgatagttc
     1381 aggaaaactt caaggagaac ttttctctca tccactatgc aggacttact atttcacttc
     1441 tactcctata ggctcaattt gacatttttc tttttcgaat tgctcatatg cctcctgaat
     1501 agtgagttcg atttatccaa atttattttt gtctattttg atgaatactt ccacgaagaa
     1561 tgaatttgca ccaattttcc ttctcttttt aagcatatta ttccagatat gattgccctc
     1621 ttgttcagta ataaattacg ctttaaacgc ttcaatcagt atatctcctg ttgtcatatg
     1681 ttctaaagaa aattcttcta catatggttt aacatctctt aggttattac ttcctaatat
     1741 cccattatgc tttttcgcta aggaaataga tgccccttct cctttaccaa taatcttgtt
     1801 actatcatga tttcttgtta aatctctata taatgcgtat tcttcagttc caatgtctat
     1861 gctcacaatc tcagctgaac ccttagctac caactgatct atcctagatt ttaaatgggg
     1921 aattgtaggt atattgattt catcatacac ctcttgtgga ataacaattt tacccaaata
     1981 gagcttttct aaaagatgtt cagtgccaac ccataaaaat actgaaatac aatctgcatc
     2041 aataaataca cgactagtca aacgacaact cccccctctt catctagccc atatacaata
     2101 tcatatctga aagcatctag taacagttcc tcatacttcc cttgcgaaat tctgttattt
     2161 tctaaaagtt gttcagttga attaatatat tatcctaatg ccatttcttt tttactttct
     2221 gacaaaggac gatataaact tgtatcatag cctaatcttg aagctgtctc tataacacta
     2281 atatccatat ttttaatttc ttctgcatca aggtatccat cattcctcaa tctatataac
     2341 atagctttat gactgatacc ataaaactga cccaatttta taatatcttc tacttcaaga
     2401 tgagttctat tggcattttc tctgatttcc tcaaccatcc tatacagtga agatgggaaa
     2461 attaaaaaat aagaagcaaa ctgatccgct tttctttcag tttcatctcc ttcaccaatc
     2521 aagataagac tgactgaact cttcttcacc tcatcataat aaagatgata cagtccatgt
     2581 gctaaagaaa atctttacct ttctaatggc atgtctgaat tgactgcaat gagactgaac
     2641 tgagttcctt tataacagac tccgctaata ttctttccga gtccataaaa taccagcgtc
     2701 aaattttcta tcttttgtac caatttaaaa atatctatcg gcgattcacc atcagctccc
     2761 aacttttttc taagatttga agctttatta cttaaatcca ttcgtgatat tcctttcacc
     2821 tttttccatt ttggcaatca tgctttgatt caaagacaaa tactcagcaa tcttatcttg
     2881 agtaagattt gaagatattc taagttcttt tattcttttt ccaacatcac atacatttat
     2941 cacatacatt tatcatattc atacacctct cataaaaaga atagcacact cttgtcataa
     3001 ttttttaaat aaaaaaatta tgacaaaaca aggaagcaat ttattgatgc tgcttaaaaa
     3061 tctaaaattg atgagttcct atcaaaaatc gtatcttcaa cctcaaaaca gtacttaaag
     3121 ctatccgact cggtttacat tgtcaaattt agattttatt tgagcataac tttctagttt
     3181 gctttctgat ttttgtttaa tatagtagca aacattgaag gaaatatctc cacaaaaaaa
     3241 cgcatactat taagcttttt caagacctaa taatatgcgc tgttctgatt tgaaagacat
     3301 tccattatta ttttactgta atcaagccat ctggctctac tgtgaattct ggcttgtctg
     3361 ccagtgttcc gtctggtttg aggtagtacc a
//
LOCUS       DAVBDG010000110         3387 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBDG010000110 DAVBDG010000000
VERSION     DAVBDG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.112"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            320..1666
                     /gene="gdhA"
                     /locus_tag="VQ526_002021"
     CDS             320..1666
                     /gene="gdhA"
                     /locus_tag="VQ526_002021"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2555682.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGIICVSEGANMPSDLDAIKVYKENGILYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDDRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2162..2332)
                     /locus_tag="VQ526_002022"
     CDS             complement(2162..2332)
                     /locus_tag="VQ526_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555683.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2345..2986)
                     /locus_tag="VQ526_002023"
     CDS             complement(2345..2986)
                     /locus_tag="VQ526_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2555684.1"
                     /translation="MTIESGQRIALVGQPGSGKSTLSKIPSGLYKIDTGKVLFDGVNI
                     NQIDKKILSQNLGVVPQDSFLLNRSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMP
                     MKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIKS
                     QGCTQIIVAHRLSTIKDADIIVVMKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
BASE COUNT         1068 a          641 c          562 g         1116 t
ORIGIN      
        1 cttttttatt ttagtctagt ctaaatattg cttttctcca aaatggtcat agttttactg
       61 gcaaatctaa catatcacgg ataaattaac aagtgatttc tgaattgcta aacattttct
      121 tttcttatag catactttaa gattttgtct ttgagaaaga tatttccaag aaaaacgttc
      181 gtttttttat ctattttcaa aaaaaccaaa aaactgaatt gaaagaattt ttagaaaatt
      241 tctgtttttt ctttacagtg aaaaaaaatt ctgctataat gtcttatgta ataaaatatg
      301 acaataaaag gagaacgata tgacatctgc taaagaatat atccaaagcg tgtttgaaac
      361 tgtgaaagct cgtaacgggc acgaggctga attcctccaa gctgttgaag aatttttcaa
      421 cactttagaa cctgtatttg aaaaacaccc tgagtatatc gaagaaaata tcttggcacg
      481 tattactgag cctgagcgcg tggtttcttt ccgtgttcct tgggttgacc gtgatggaaa
      541 aattcaagta aaccgtggtt accgtgttca attcaactca gctgttggac catacaaagg
      601 tggacttcgt ttccacccaa ctgtaaacca agggatcttg aaattcctcg gatttgaaca
      661 aatctttaaa aatgtcttga ctggacttcc tatcggtgga ggtaaaggtg gatcagactt
      721 cgatcctaaa ggtaaaacag atgctgaagt gatgcgcttc tgccaaagct tcatgactga
      781 attgcaaaaa cacatcggac catcacttga cgtacctgct ggtgatatcg gtgttggtgg
      841 acgtgaaatt ggttaccttt acggtcaata caaacgtctt aaccaatttg atgctggtgt
      901 cttgactggt aaacctcttg gatttggtgg tagcttgatt cgtccagaag caactggtta
      961 cggtttggtt tactatactg aagaaatgct caaagctaac ggtaacagct ttgctggtaa
     1021 gaaagtcgtt atttcaggtt ctggtaacgt tgctcaatat gctcttcaaa aagcaactga
     1081 actcggtgca actgttattt ctgtttctga ctcaaatggt tatgtcatcg atgaaaatgg
     1141 tatcgacttc gatcttttgg ttgatgttaa agaaaaacgt cgtgctcgtt tgactgagta
     1201 tgcagctgaa aaagcaactg caacctacca cgaaggtact gtatggactt acgctggaaa
     1261 ctatgacatt gctcttccat gtgccactca aaacgaaatc aacggtgaag cagctaaacg
     1321 tttggttgct caaggcatta tctgtgtatc tgaaggtgcc aacatgccga gcgaccttga
     1381 tgccatcaaa gtttacaaag aaaatggtat cttatacgga cctgcaaaag ctgccaacgc
     1441 tggtggtgta gccgtttcag ctcttgaaat gagccaaaac agtcttcgcc tctcatggac
     1501 tcgtgaagaa gttgatgacc gtctcaaaga catcatgaca aacatcttca acacagctaa
     1561 aacgacttca gaaacatacg gtcttgataa agactacctt gcaggagcta acattgctgc
     1621 ctttgaaaat gtagcaaacg ctatgattgc ccaaggtatt gtttaagatt tatttgctta
     1681 atcctcaatc actacgattg gggattttta gttgagttag acagttggga aattctaatt
     1741 aaatgaaata aaaactgaac aaattgattg cggaattcaa accaatttct aacatatttc
     1801 tagaaattaa tttgactttt ctaatcgatt tgttcatatc ttatttcaat ttactatact
     1861 tacttaaccc tttttatact caatgaaaat caaagagcaa actaggaagc gagtcgcagg
     1921 ctgtacttga gtacggcaag gcaaagctga cgtggtttga agagattttc aaagagtatt
     1981 aaacaaaaag aaaaaacgct actcctaaga atagcgattt aatcatgttt ttaagctact
     2041 actgtagttg ctctattatt taagtgttat gaataacttc aatatcttta aagctttttg
     2101 ttagatttat aatttcaact atagccatct ctactcctta gtttacagca aaaaattatg
     2161 cttaattctt attgtttttg taaacaatca gcgaacccaa tactattcca ttcaacaatc
     2221 ctaaagaaat accatattca actggaaaat ctaaaaaaat tgcaaacact attccaatca
     2281 tcacccctag tgaggctgtt gttaaaatta catattcatt ttctttcttc attttcttta
     2341 cacctcattt cctttttgta tataagctgt agtactctcc accaagatcc attaagtact
     2401 tatgatttcc tgactcaaca atcttaccac ctttcattac aacaataata tccgcatcct
     2461 taatcgttga caatctatga gctacaatta tttgagtaca gccctgactt tttatatact
     2521 ttgttattct ttcctcatta atagtgtcta aagcactagt tgcttcatct aaaattacaa
     2581 tactaggatt atttattaat gcacgtgcca gtgctatccg ttgtctttga ccacctgaaa
     2641 tatttgaacc catctctgag atgatagtat taaatttcat cggcatagcc atgatttcat
     2701 catagatttg aactgcttta caaacttcct ctatcttttg tgaagtaact tcgtgcttta
     2761 aagttatgtt atcaagaata cttctgttca ataaaaacga atcctgtgga actactccta
     2821 aattttgact taatattttt ttatctattt gattaatatt tacaccatca aataaaactt
     2881 ttcctgtatc aatcttatat aatcctgatg gtatttttga taatgtactt tttcctgacc
     2941 ccggttgacc gactagtgca attctctgtc ctgactcaat agtcaaattt aaattttcta
     3001 ttacaggagc tgagtctttg gaataagaga aagataggtc cttaatatct attctacttc
     3061 aaacgtttct aaactatact ccggaaaata atttaaccaa taactatctt tcatcgtatc
     3121 ctccaaccag tactaagagt ctgggacaat agtctcttat ctccaaaaaa gaagcggatt
     3181 tgccgttgct tttttgcatg gttgcgatag tcttggtaaa atagaattgc ccaataaacc
     3241 atttagaagg gctatcccat gcatattcac tataacacaa atcaaacaac tttaccacta
     3301 gaaatcagtt ctttcttgcc gcaagaccat ctcgtcttta ctattgaaaa agtggtgaat
     3361 accgatgaaa aggaggtaaa ccatctg
//
LOCUS       DAVBDG010000111         3376 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBDG010000111 DAVBDG010000000
VERSION     DAVBDG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.113"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..83)
                     /locus_tag="VQ526_002024"
     CDS             complement(<1..83)
                     /locus_tag="VQ526_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2555685.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFKE"
     gene            226..537
                     /locus_tag="VQ526_002025"
     CDS             226..537
                     /locus_tag="VQ526_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555686.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            541..1257
                     /locus_tag="VQ526_002026"
     CDS             541..1257
                     /locus_tag="VQ526_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2555687.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1387..2202)
                     /locus_tag="VQ526_002027"
     CDS             complement(1387..2202)
                     /locus_tag="VQ526_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2555688.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2199..3104)
                     /locus_tag="VQ526_002028"
     CDS             complement(2199..3104)
                     /locus_tag="VQ526_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2555689.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMFFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
BASE COUNT         1124 a          579 c          698 g          975 t
ORIGIN      
        1 atctctttaa aagtcgtgcg ttgaacacca acaagacgct taaatcgtgc atcggttagt
       61 tgtttacttg cttcataatt catagaacta ttataccata ttttgtttcg caggaagtct
      121 aatgctaaaa tcttatcagt gagaagattt cttaaagttt attctttgta gtttcatctg
      181 tattagtaca taatttaact tagagaaaat aggaggacta atcgaatgga attaaaagat
      241 tttacagaaa aagaacagga aatgattaag aaaaggctta caatgtctaa tattagtgac
      301 aaagaaacta ctgagaagat tctggcgcta gtcccacaag acttgattaa gcgaatcccg
      361 ttttttgtca gaaaacatgc tacaacacgt acgattaaac gcatttcaat tgaataccct
      421 gaactctacg ctgtagctca aacaagtggt gagattccag aaaaaaaacg cgaagaattg
      481 cgtcagatta tcacaactat ctttgaacaa aagatgaata agcatagtat taagtagaga
      541 atgaaaaaat attttattgg cggtttggga agcaatgcct atcatagcaa ggattttctt
      601 caagaactag attcgcaggt ctattttcta aatccatatg aaaagcatct tcgagatgaa
      661 acagaattga aatcatggtt taaaaatgag attgtagagg aagaatctat ctgtctgata
      721 ggtcattcta ttggaggcga tttagctcgt tatttcgcat cggaatttga agaagtaaag
      781 aaactgattc ttttggatgg tggctatcta gatttagata agattttacc tttggataca
      841 gagttagagg aaactaaaaa ttatatcaaa tctcaaatta tttcggactt agatgttctt
      901 acttctaaag aaaaatctga agcaaagcat tggtcagaaa atatggagaa agctgtaaga
      961 cagtcctatc actggaatgt tgagtataat agatatgagt tggctataaa ttatgaaaat
     1021 atagaagcga tactccgcct acggagaaaa atacaagctt ttaagagaga agtgggagat
     1081 accttgttta ttagtcctcg ctatcctaat gaagctacat ggagagagga agccctaaaa
     1141 gaattgccag actattttga tactattttt ttagaaaact ttggccatga gctttatact
     1201 caagcaccta aagaaatcgc tagtctaatg aatgagtggc tcgcttattt tctatgagct
     1261 acttgtattg ctggaaatgg tataagatga aaatctacaa gtcactaatt ttcatataac
     1321 caaaaaacga acaggctctt tttctgttat tcagaagaga acttgttcgt ttttatcttg
     1381 attttcttaa aaagctctat atacataggg attttctggt ttatcgactt tggtaaagct
     1441 gtcgatttcc aaggttggaa ggttttgttt gagttcttta tggtagtgat tgaccagttt
     1501 tcctttggct gaaatccaag tgttgttttc atactgccgg gtattgccct tggtcagcaa
     1561 tccatagaca ccagaatctg cgatgcagtg aataatgccg aagcggaaca gaaattggct
     1621 attggcatga ctggggtcat tatagacaaa gcctgtgaac tggattgtct taccctcaaa
     1681 ttcatctgga tagtcgtaga tagcctccat gacctccata tagttttcat tagtgatctg
     1741 aatactatct tgggataagt atttatccgc cgccgttcgc atttcctttt cataggctga
     1801 ttttgaaaaa taagaactgg tatctggttt caaatattgg cttgtcgtcc cttcgcttgt
     1861 ctgaatggct agatccgttc cttccgatag ggggaaatga taacctttag cagaaacggt
     1921 ctgagaatcc aagctaacag ttgggaaagt taagccgatg acaagtggaa tagccagaag
     1981 agaaatactc gtcatcttgg ctaatcggct gttcagatga ctgtgggttt tgacttgctt
     2041 catccagata tacaattgaa cgatagccaa gataaaagaa agcaccatgg agatataggc
     2101 cagataggaa tagtgcatgt tgatgtactg gtttagtttg cccgacagat ggaggtaaat
     2161 agtcagttca aaatagccag ctaaaactaa aaatcgaatc ataggatgac tccaatcaag
     2221 agagaataga ctaagacgac aagagttaca attgttatga agtgactgat aaatcgtgct
     2281 ttcaagtaat ttttcatcat gagaatattt ttgatatcca gcattggacc aatgacgaga
     2341 aaggccagaa ctggtgccaa accgaaactc gagagaagag aagcacctat aaaggcatcc
     2401 gcctcactac agagcgaaag aagaaaggct aaaatcatca agagcaggat ggcaaaaaga
     2461 ggggtcgcac tgatagaggt cagaatccga gtcggaacgt agacctgtat tatagaagca
     2521 aagaggcagc caaataccaa ataacgcccc gtatcaaaaa attcatcaat ggcctgcaca
     2581 aagacttgaa aaactttttt tgcagaactc aagtaagaaa aatcatgctc atgacaagcc
     2641 agacgatttt ctttctgaat cggttcttgc cagaaaaatc ctagaaaaat tcctagtatt
     2701 acagccacaa gaatggaacc cagagctcgt aatagggcga catggaagga gttgccaaag
     2761 gcagaatagg tcgcaaaaag aacaatggga ttgataacag gtgctgtcac aagaaaagga
     2821 acggccgtgt aacttggaac ctttttttcc agaaaacgat tgatgatggg gacgattcca
     2881 cattcacaag aagggaaaaa cataccgacg aaggtcccaa aaaagattct cccccaacga
     2941 tttcgaggga gaaaatgata aaccttgtca ggtgtgatat aaacttcaat cagccctgag
     3001 acaatgcttc ctatcagaac gaagggaagg gcttcaatga taatggagag aaaaatggct
     3061 ccagtttgaa gtacactaga tgggagttgt tgaaaagcag tcatctagtt tttcttttct
     3121 gctttggctt ttttctcttt gtcatcaggg aaagtaactt gtttcaaatc agggagttta
     3181 gcaaactctt caaacatctt gtccaagtcg tctcgttttg taaattttac agccataggt
     3241 gtacctcgat tctaaattct acctctatta tactattatt ttgcggaaaa gggagttttt
     3301 taatttgttt tcgatagttg atactcttcg aaaatctctt caaaccacgt cagcttcgcc
     3361 ttaccgtagt acggtt
//
LOCUS       DAVBDG010000112         3347 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBDG010000112 DAVBDG010000000
VERSION     DAVBDG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.114"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(192..824)
                     /locus_tag="VQ526_002029"
     CDS             complement(192..824)
                     /locus_tag="VQ526_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2555690.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(846..1718)
                     /gene="sdaAA"
                     /locus_tag="VQ526_002030"
     CDS             complement(846..1718)
                     /gene="sdaAA"
                     /locus_tag="VQ526_002030"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2555691.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1727..2398)
                     /gene="sdaAB"
                     /locus_tag="VQ526_002031"
     CDS             complement(1727..2398)
                     /gene="sdaAB"
                     /locus_tag="VQ526_002031"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2555692.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2640..3212
                     /locus_tag="VQ526_002032"
     CDS             2640..3212
                     /locus_tag="VQ526_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2555693.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3264..>3347)
                     /locus_tag="VQ526_002033"
     CDS             complement(3264..>3347)
                     /locus_tag="VQ526_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET2555694.1"
                     /translation="LLELTEETSMLSHLKKEVEIYYQAKER"
BASE COUNT          979 a          680 c          725 g          963 t
ORIGIN      
        1 gtatatggtt actgacttcg tcagttctat ccacaacctc aaaacagtgt tttgagctga
       61 cttcgtcagt tccatctaca accttaaaac agtgttttga gcagcccgca gctagtttcc
      121 tagtttgctc tttgattttc attgagtatt acttcatttt cttctgaaat agagttgtcg
      181 cccagtctat gctattgaaa atacgccaaa acttctaagg gtttgtgagc gatataatca
      241 ggttgatagt ttagtagatc tgcttgctct ccaaatcccc aagtgacggc caatttctga
      301 atacctgttt ctcgagctcc cagcatatca aacttggtat ctccgatgat gatggcttgt
      361 tctggtgcta gttgatgtgt ctgtaaggct tggtgaatga catctgcctt atggggtgct
      421 tcagggctag aaccataaat accatcaaag aaatgatgga tttccaagtt tttggccatg
      481 tcttgagcag tagatgtatt ctttgtcgtg gtgatgtaga gtggataact gctcgataac
      541 tcctcaagca agtctataat ctgaggaaag agttgagctt catagatgcc ttttgcctta
      601 tagtaagaac gatatatctg catggcttca gaaatttggt ctttggacag gcaggtcgca
      661 aaactacttt cgagaggtgg tcccataaaa ccacgaatag ttttggcatc agggctaggc
      721 acccccagct ctttaaaggt ataggtaaag gcattgtgaa tcccgataga actatcaacg
      781 agggttccat ccaaatcgaa aaaaatcgct gtgatagagg tcatggtttc tcctatttga
      841 taagcttatt ctccgaaaat ctctttttgg aggcgacgac cagtaggggt ggtagcgagt
      901 ccaccttcag ctgtttcacg aaaggcagtt ggcatgcttg ctcctacttg gtacatggca
      961 tcgatcactt catccacagg gattttagat tcgatacctg ccaaggccat gtctgctgcg
     1021 atgaaagcaa agctagctcc catggcatta cgtttgacac agggaacttc gaccaaacct
     1081 gcaacagggt cacagatgag gcctagcata tttttaatga caaaggcaat agcttgactg
     1141 gcctgataag gtgttccacc tgcagccaga gtcaaggcgg cagcactcat agcagaggct
     1201 gaaccaactt cagcttgaca cccaccctca gcacctgaga tggaggcatt gtttgcgatg
     1261 actagtccaa aggcaccagc agcaaagagg aaatccaatt gttgctcgtg gctgaggtct
     1321 aatttttcaa tagcagcagt gagaacggaa gggagacaac cggcacttcc tgcggtagga
     1381 gtagcgcaga ccaagcccat tttagcattg tgttcattga ctgcgatagc atttcgggca
     1441 gcagagagaa tcgtgtaatc tgataaagtt ttttcgtttt tgatgtagtg atccaatttg
     1501 gcagcatctc cacctgtcag gccactacga gatttatttt cattgaggcc aagttggaca
     1561 gaggctttca taacttccag attgcgttcc atgagaagga agacttcttc acgttcgcga
     1621 ccggtcaatt caaactctgt tgtaatcatg agttctgcga catttccttg aaagtccaga
     1681 tctgcttgct cgaccaattc tttgatagaa taaaacatgc ttcctcctat ttaaagaaat
     1741 tgacattgtg gagatgaggg atttttcgaa tttcttcgat agcctcatca cagttgcgac
     1801 tgtcaacttc gataatcata atggcttttt caccagcttt ttcacgagtg acattcatct
     1861 gggcgatatt gataccatag cgggaaagcg cctctgtaac aagggcaatc atacctggaa
     1921 tatcttgatg aacgatgatg atagtcggtg tattcatatt gagagagacg gcaaaaccat
     1981 tgagttcggt tacctgaata tttcctccac cgatagaaat accagtcacg ctgatggtct
     2041 tgtgggcatt tttaacagta attttagtgg tgttagggtg aggggcattg ctgtctttct
     2101 gaatggtcca gacaatcttg ataccacgct tgtgggcaat ttccagacta tttggaattt
     2161 caggatcatc tgtatccatt cctaaaatac ctgcaacaag ggctaggtct gttccgtgac
     2221 cacgataggt cttggcaaat gagttaaaaa gttggaattc aacttctgtc ggagtatcat
     2281 caaaaatgga agagacaatc ttcccaatac gaacagcacc agcggtatgg ctactagatg
     2341 ggccaatcat aactggtccg atgatatcaa agacagattg aaaacgaagt gatttcatca
     2401 gtttcccctt ataaaaattc ttatctctat tatatcaaag aatgaggggc ttggctttaa
     2461 ttgtggatga aaacctttct aatacctcaa atagcataaa aatagtatct tttatgacaa
     2521 aaaacacctt atttagggaa ataaaaaata attttgtaat atttctacat aaaagtgtca
     2581 agaaacggta atatttaaag ggtatgatag aactatagaa agaaggagaa ttttcgaata
     2641 tgaaatcaat aactaaaaag attaaagcaa ctcttgcagg agtagctgcc ttgtttgcag
     2701 tattttctcc atcatttgta tctgctcaag aatcatcaac ttacactgtt aaagaaggtg
     2761 atacactttc agaaatcgct gaaactcaca acacaacagt tgaaaaattg gcagaaaaca
     2821 accacattga taacattcat ttgatttatg ttgatcaaga gttggttatc gatggccctg
     2881 tagcgcctgt tgcaacacca gcgccagcta cttatgcggc accagccgct caagatgaaa
     2941 ctgtttcagc tccagtagta agtgaaacag ttgtttcaac tgtaagcgga tctgaagcag
     3001 aagccaaaga atggatcgct caaaaagaat caggtggtag ctatacagct acaaatggac
     3061 gttatatcgg acgttaccaa ttaacagatt catacctgaa cggtgactac tcagctgaaa
     3121 accaagaacg tgtagcagat gcctacgttg caggacgtta cggttcatgg actgctgcta
     3181 aaaacttctg gcttaacaat ggctggtatt aagaactaac gaacaaataa tatgtaaaga
     3241 gtcgaggaaa tccctcggct tttttatctt tccttcgctt ggtaatagat ctctacttct
     3301 tttttgagat gagacagcat agaagtttcc tcggttagct ccagaag
//
LOCUS       DAVBDG010000113         3242 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBDG010000113 DAVBDG010000000
VERSION     DAVBDG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.115"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            118..2247
                     /locus_tag="VQ526_002034"
     CDS             118..2247
                     /locus_tag="VQ526_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2555695.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            2234..2683
                     /locus_tag="VQ526_002035"
     CDS             2234..2683
                     /locus_tag="VQ526_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2555696.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTQRYRCGLCRGKLLLINQPED"
     gene            2742..3014
                     /locus_tag="VQ526_002036"
     CDS             2742..3014
                     /locus_tag="VQ526_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2555697.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT          947 a          633 c          734 g          928 t
ORIGIN      
        1 acaacctcaa aacagtattt tgagcagcct acggctagct tcctagtttg ctttttgatt
       61 tttattgagt atgaaaagga ttggagaaag ccttccagct tgtggtataa tagatttatg
      121 gataaaaaat atgaaaaaat ctctcaggat ttgggagtga cgttaaagca aattgatacc
      181 gttctaagtt tgacagctga aggggcgact attcccttta tcgcgcgtta tcgcaaggac
      241 atgactggta gtctggatga ggtggcgatt aaggctatta ttgatttgga taaaagtctg
      301 acaaatctca atgaccgtaa ggaagctgtc ttagctaaga ttcaagaaca aggtaagttg
      361 accaaggaat tggaagaagc tatcttagtt gccgaaaaat tagcagacgt tgaagaactc
      421 tatcttcctt ataaggaaaa gcgtcgtacc aaggcaacca ttgcccgtga agctggactc
      481 tttcctcttg ctcgcttgat tttgcagaat atagttgact tagagaaaga agctgaaaag
      541 ttcgtctgtg aaggatttgc gactggcaag gaagccttga ccggtgcagt tgatattttg
      601 gtcgaagcct tatcggaaga tgtgaccttg cgttctatga cttatcagga agtgctgaga
      661 cactctaaac tcacttctca agccaaggat gaaagtcttg atgaaaagca ggtttttcag
      721 atttattatg atttttcaga gacagttgga actatgcaag gctatcgtac cttggctctc
      781 aatcgtgggg agaaacttgg tgtcttgaag atcggttttg aacatgcgac ggaccgtatt
      841 cttgccttct ttgctactcg tttcaaggtg aaaaatgctt atattgatga agttgttcag
      901 caatccgtta agaaaaaggt cttgcctgct atcgagcgtc gtattcggac agaattaact
      961 gagaaagctg aagaaggagc tatccaactt ttttctgaca atctgcgcaa tctcctcttg
     1021 gttgctccac tgaaagggcg cgtggttctt ggatttgacc cagcctttcg tacaggtgcc
     1081 aagttagctg tcgtggatgc aacaggaaaa atgctgacaa ctcaggttat ttatcctgtt
     1141 aaaccagcat cagctcgtca aatcgaagaa gccaagaaag atttagcaga tttaattggt
     1201 caatacggtg tagagattat tgccattgga aatggaacgg ccagtcgtga aagtgaagct
     1261 tttgtagcgg aagttctgaa agatttccct gaagtcagct atgttatcgt taatgaaagt
     1321 ggtgcttctg tctattctgc cagcgaactt gctcgtcagg agtttccaga cttgaccgtt
     1381 gaaaaacgct ctgccatttc tatcgcccgt cgtttgcaag atcctcttgc ggaattggtc
     1441 aaaatcgatc ctaagtcaat tggtgtcggt caataccaac acgatgtcag tcagaagaaa
     1501 ctatctgaga gtctggactt tgttgtcgat acagtggtta accaagttgg tgtcaatgtc
     1561 aatacagcta gcccagctct tctttcacac gtagctggac tcaacaaaac tatctctgaa
     1621 aatattgtca aataccgcga ggaagaagga aaaatcactt cacgcgccca aatcaagaaa
     1681 gttcctcgtc tgggagccaa ggcctttgag caggctgctg gtttccttcg tatccctgaa
     1741 agtagcaata tccttgataa tacaggagtt cacccagaga attacactgc agttaaggaa
     1801 ctcttcaaac gtttagatat taaagaccta aacgaagaag cccaaagcaa actcaagtcc
     1861 ctttcagtca aggaaatggc tcaagagcta gacctcggtc cagaaaccct taaagatatc
     1921 attgcagatc ttctcaaacc aggtcgagat ttccgtgatt cctttgacgc acctgtgctt
     1981 cgccaagatg tcctagatat caaagaccta gtggtaggtc agaagctaga aggtgttgtg
     2041 cgtaacgttg ttgacttcgg tgccttcgtt gatatcggga ttcacgagga cggcttgatt
     2101 catatttctc atatgagtcg caagtttatc aaacacccta gtcaagtggt gtcagttgga
     2161 gatttggtga ccgtttgggt caagaaaatt gatactgaac gtgaaaaagt taatctgtcg
     2221 ctcctcgctc caaatgaaac tgactgatta tgtcaagcag gtttcactag aagacttcgg
     2281 gagagctttt atccatcatg tccagtggaa taggcgtcta cgttcgacag gtgggcgatt
     2341 tttccccaaa gatgggcatc tggattttaa tcccaaggtt tatcagaaac tgggtatgga
     2401 ggtttttagg aaaattgtcc gacatgaact ctgtcattat cacctttatt tccaagggaa
     2461 gggctatcaa cacaaggatc gggattttaa ggaacttttg aaagcagtgg atggattaag
     2521 gtttgtacca tccttgccca atagtaactc taaaccactc aagctttatc gttgtcaatc
     2581 ctgccagcaa aggtatcagc gcaagcgtag gattgatacc caacgctatc gctgtggact
     2641 ttgtcgaggt aaattgcttc tgataaatca gcctgaggac tgatgaaagc cgagcctccc
     2701 cgtgatatac tatgtctaac ctagcagaaa gaggaaatat catgaacagt aaattttata
     2761 aaatgagacg caatcgcatg atttcaggag ttctggcagg tctgtcagac aagtggaatt
     2821 ttgatgttac cctagttcgt ttcttgtttg ccatttttac agttgcaaac tttggacttg
     2881 gcgtgattat ctatatcatc ctagcttcta tcatgcctac caaggaagaa atcgaagcag
     2941 aaatgtacgg aacaggccca cgtaaacgca aagaagccca agccattgac gacaatgaag
     3001 gctggttttg gtgaggttct acagaagatt tggaataagc aaaaatcttt aaatcaatag
     3061 tctaaactgc ttgatttaaa gcttttttac ttttatactc aataaaaatc aaagtgcaaa
     3121 ctaggaagcc agccacaggc tgtaatttag tcggcaatgt gaagttgaca tagtttgcac
     3181 tttgattttt gaatagtatt aataatattt cgccagcttc atccacaaac tatagtggat
     3241 tg
//
LOCUS       DAVBDG010000114         3232 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBDG010000114 DAVBDG010000000
VERSION     DAVBDG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.116"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(180..791)
                     /gene="rpsD"
                     /locus_tag="VQ526_002037"
     CDS             complement(180..791)
                     /gene="rpsD"
                     /locus_tag="VQ526_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2555698.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(986..2038)
                     /locus_tag="VQ526_002038"
     CDS             complement(986..2038)
                     /locus_tag="VQ526_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2555699.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2035..2733)
                     /locus_tag="VQ526_002039"
     CDS             complement(2035..2733)
                     /locus_tag="VQ526_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2555700.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2900..>3232)
                     /locus_tag="VQ526_002040"
     CDS             complement(2900..>3232)
                     /locus_tag="VQ526_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2555701.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGLLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT          902 a          733 c          617 g          980 t
ORIGIN      
        1 attataccac attgtgtact atttttggtt cattttacta tatcactttg gactatatcg
       61 gtctagttat gtctcaacga ttatagctgg atactaagag aaatcaaaaa agcactaagg
      121 gagaacgtcc caaagtgctt aatatcaagg cctcaaagcc tgtcttattc aataaaatat
      181 tacaacatct tgttgtagaa ttcaacgaca agtgcttcgt tgatttctgg gttgatttcg
      241 tcgcgttctg gcaagcgagt caatgaacct tccaactttt cagcgtcgaa tgatacgaat
      301 gctggacgtc caagagtagc ttctactgct tcaaggattg ctggaacttt caatgatttt
      361 tcacgaactg agatcacttg acctggagtt acgcggtatg atgggatatc aacgcgtttc
      421 ccgtcaacaa ggatgtgacc gtggtttacg aattgacgag cttgacgacg agtagtcgcg
      481 agaccaagac ggtaaacaac attatccaaa cgacgttcca aaagaagcat aaagttgaaa
      541 cctaggattc cacctttgat ttttgtagct tgtacgaaca agttacggaa ttgtttttca
      601 cctacaccgt aagtgaaacg aagtttttgt ttttcagcca attgcaaacc gtattctgac
      661 aatttagaac ggttgtttgg tccgtgttgt cctggtacgt agttacgacg tgccaattct
      721 ttacctgtac ctgtaagtga aaggccaagg cgacgagctt gtttccaaga tggtcctgta
      781 taacgtgaca tatgaatgtc ctcctgatat aaataatatt tcggtggaaa tagtcactta
      841 gaaagccctg attcgtgcag atgcccttcg cctaaacagc caaggttact tatcataaga
      901 cacctgttga cgagcttcat gctttcctgc tgatatttca cacaaaatcc attctaccat
      961 gaatagctgg atttgtaaag gggttttagg ctttattttc actaccagag aggttgagaa
     1021 cgagggtaaa ggtacttcct agaccgtact ggctgctgac tgtgatttcc ccacccaatt
     1081 gatgggccaa ttcacgcgca atcgcaagtc ctaatccatg accacctgtc ttcatgttac
     1141 gcgaagtttc gacacgataa aggcgtttga aaatattttc caaatcctct ggggcaatgc
     1201 cctgcccttc atcggtcaca ctgattgaaa gctggtcctt ctccagctta gccaccactt
     1261 ccagcttggt tcctggagca gaatatttaa aagcgttatc gaccagattc accaagatac
     1321 gagaaagctt agcataatct ccctcaatcc gggcagactc tgggattacc tgcaagtgga
     1381 catctcttct ctcctgctca atcaaaaact gaaattcact catgcactca attaagagct
     1441 tgtccagaaa aatactgtct ttactggtag tttccacctg atttctagct gtgtttaggg
     1501 tcaaaaaatt caactcctca accagtttat tgagcctctc cgtctggcgt ccaatggttg
     1561 ctagataatg agcttgctcc gactccttga taatcccatc caaaatccct tctaccgtcg
     1621 cttggatcga agtgatagga gtcttaatat catgcgacaa ctgagcaatc atcaagccct
     1681 tttctcgttc gctttcttcc aaggaatcaa agcttacctg caaatcatgg gacatctcat
     1741 taaaagtttg ccctaattgc tgaaattcta caggaccttg aacctccaaa tttgaaggaa
     1801 aatccttggc cgctacccgc ttggcatgct ccttgagttt gcccaacgac gtaaagactg
     1861 gcaataggag aaagagacta atcccagcac cgacaaggct ggcaacgatg gtcatcccaa
     1921 gcaaaaagta aatctcgcct ttcgcaatca gcattttttg aacagcccaa aaaacaacca
     1981 aaatggttaa gagggttgaa ataatatatc caaccaaaat ataacttttt agtttcatgt
     2041 ttgtcctctc ggtttctcta tcttatatcc caacccccaa actgtcttaa tagtgggcgt
     2101 ttggtcacta ctatattttg ccagctcctg tcgaagagca tggatatgca cattcaaggt
     2161 attggtgtca tccacgtagt cttctttcca gatcttttca tagaggtctg tcttggagaa
     2221 aactcgctct ggattgctag ctaaaatcca cagcaattca aatgatttaa cagttaaatc
     2281 cagcatttct tctcctattt gaacttcatg actactatga ttcattttta gattgccaag
     2341 ggaaatcagc tctgtttcgc ccccacgatg aaggcggcgc aaaatattgt ggacacgcaa
     2401 aaccagttca cgtgggctaa aaggcttagc aataaaatca tctgctccca agctcaggcc
     2461 gtaaatcttg tcctgttcac tggtcttagc agtaataaat aggaaaggct gctctggtga
     2521 taagtattga acctcactga ttaaatcata accatccatc cgaggcatca tgacatctgt
     2581 gataatcaaa tcaatcggtt ttttcttgaa cagctctagc gcttccagtc catcatgggc
     2641 taccaagacc tgataacctg cctgaattaa gtatctctga tgaatatctg tgatttctac
     2701 ctcgtcgtca acgagtaaaa ttgtctttcc catctgtctc tcctttgata aaaacagtgc
     2761 tatactgctt taagtataac actattttta ttgttttacg atgattttaa tctgctattg
     2821 aaagaaagtc ctgattgctg attgagttgt taaactaatc ttagtcagcc cactatccat
     2881 catttatttc cttagctgat tagttttctt cttttttgtt tttcaaacct agaccaccga
     2941 gtaaacctgc aagcgcaagc ccaagtaaac caatacttgc cattgatgtt tgagtctcac
     3001 cagtatttgg tagcatagct ttatcctctg acatcatcgt atcagacatc ttgttagcag
     3061 aagcagccat gttttcacct gccatcgtgt tggtagaact tgtcatggtg tcagcaggca
     3121 tgctatctgt agtacctgta gcatgattgt gattcatcgg agtcacgcca gaaccagagt
     3181 tagaaggtga taatgaacca tttgctgtgt ctgatgtttc tttaacattt at
//
LOCUS       DAVBDG010000115         3113 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBDG010000115 DAVBDG010000000
VERSION     DAVBDG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.117"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            27..542
                     /locus_tag="VQ526_002041"
     CDS             27..542
                     /locus_tag="VQ526_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2555702.1"
                     /translation="MLCIGELTILQILYCASLISFLWMYGQRKQVVKVNMKTRMKWLG
                     IGFVSLLIISLCFSLIHAQGTTNQANLIGLQHQVPWFSFLLFLINASMVEEFLYREIL
                     WNLVRKLDIRVALTSVLFALVHHPGTILAWCLYVSLGIFLGMVRYKSDLWGSMGLHLV
                     WNLSVYVLFFL"
     gene            672..1262
                     /locus_tag="VQ526_002042"
     CDS             672..1262
                     /locus_tag="VQ526_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HET2555703.1"
                     /translation="MVDKREKLMNSFNQYGFLTFKQVIDENLHYKTLLKMVTEGKIDA
                     EEKGLYRLPDIYLDEWFVIQYRFPKGIFSLETALWLHGLSLTIPFNMTMSFPYGTNTK
                     NIKEADICPIILRSHYSEGIIEIERLPGQFIKVYEIERVLVECLRPVHQVDLQIIAPA
                     FKKYFQQNQIHLHKLFYYAQLFKVTDKLQSYTEVLS"
     gene            1262..2098
                     /locus_tag="VQ526_002043"
     CDS             1262..2098
                     /locus_tag="VQ526_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HET2555704.1"
                     /translation="MFSNANSFKAKIKNISKDKGIPAQQVQQHYLIEQVLKLISTSSY
                     RDSFIVKGGYLIGQMIGLDKRTTMDLDVTLKGTEMSRENLIDIFEEILCSKTDGFSFS
                     VDKLEPIRQDDEYGGFSLKLNATFDTLKEVVFIDITTGDKITPREITYSMTSIFTNES
                     IKIWTYNLETVLAEKLETIISRGLASTRPRDRYDLFTLYKLRKEEINLEVLKNALENT
                     AKKRKSKDTIYNWEEQVRGIEISDYQKELWIRYQRQFKYAKDISFDNSVQVIREIMQQ
                     IF"
     gene            2381..2599
                     /locus_tag="VQ526_002044"
                     /pseudo
     CDS             2381..2599
                     /locus_tag="VQ526_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2899..3113)
                     /locus_tag="VQ526_002045"
                     /pseudo
     CDS             complement(2899..3113)
                     /locus_tag="VQ526_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1073 a          449 c          558 g         1033 t
ORIGIN      
        1 tattggtatc cctatactta ctgaaattgc tttgtattgg tgagttaacc atactacaga
       61 ttctctattg tgcgtcgctc atttcttttt tatggatgta tggtcaacga aaacaagtgg
      121 tcaaggtcaa tatgaaaact aggatgaaat ggcttggtat tggattcgtt agcctactga
      181 ttataagcct atgttttagt ctgattcatg ctcaaggaac cacgaatcaa gcaaacttaa
      241 ttggccttca acatcaggtt ccttggtttt catttttatt gttcttaatc aatgcgagta
      301 tggttgaaga atttctgtat cgagaaattt tatggaactt ggtcagaaaa ttagatattc
      361 gagttgcttt gacaagtgtt ttatttgcct tagtacatca tccaggaacc attctagctt
      421 ggtgtttata tgtttcactt gggatctttt tagggatggt gcgctataaa tcggacttat
      481 ggggaagtat gggtctacat ttggtgtgga acctatcagt ctatgtctta ttttttcttt
      541 aataatagct ggtctgtctt acgtatggat taattttgaa ataaaaaaac taaatgaatt
      601 gattttaaag aaataaaatg ctaacatata tagaagatgt tagcatttta tttctaagaa
      661 aggagaatgg tatggttgat aaaagagaga aactgatgaa ctctttcaat cagtatggtt
      721 ttttaacttt taaacaagta atagatgaaa atttacacta caaaacctta ttaaaaatgg
      781 ttacagaagg aaaaatcgat gctgaagaaa aagggttata tcgcttacct gatatttatt
      841 tagatgagtg gtttgtcatt cagtatcgat ttccaaaagg aatcttttct ttggagacag
      901 cactttggtt acatggttta tctttgacta tcccttttaa catgacaatg agttttcctt
      961 atggtacgaa taccaaaaac attaaggaag cagatatatg tcctattatc ttacgctctc
     1021 actatagtga aggaattatt gaaatagagc gtcttcctgg tcaatttatt aaagtttatg
     1081 aaattgaacg agttttggtt gaatgtctaa gaccagttca tcaggtggat cttcaaatta
     1141 ttgcaccagc gtttaagaaa tattttcaac agaatcaaat tcatttacac aaattatttt
     1201 attatgccca actatttaaa gtaactgata agttacaatc ttatacggag gtgctatctt
     1261 aatgttttca aatgcgaata gctttaaagc aaaaatcaaa aatatttcaa aagataaggg
     1321 aattccagct caacaagtac aacaacatta tttaattgag caagtgttaa agctgatttc
     1381 tactagttct tatagagatt ccttcattgt aaaaggaggg tatctaatag gtcaaatgat
     1441 tggactagat aagcgaacca caatggattt agatgtcact ctgaagggaa ccgaaatgag
     1501 cagagaaaat ttgattgata tctttgaaga gattctttgt tctaaaactg atggtttttc
     1561 attttcagta gataagctag aacctattcg ccaagatgat gaatatggag gattttcatt
     1621 aaaattaaat gcaacttttg atacattaaa ggaggttgtt tttattgata ttactactgg
     1681 tgataagatc acaccaagag aaattactta ttcaatgact tctatcttta ctaatgaaag
     1741 catcaagata tggacatata atctagaaac tgtacttgct gaaaagttag aaacaatcat
     1801 tagtagggga ttagcttcaa cacgcccacg tgatcgatat gatcttttca ccttgtataa
     1861 acttagaaaa gaagagatta atcttgaagt attaaaaaat gcacttgaaa atacagcaaa
     1921 aaaacgtaaa agtaaagata ctatttataa ttgggaagaa caagtgagag gaattgaaat
     1981 ttctgattat cagaaagagc tatggattcg ttatcaacgc caatttaagt atgctaaaga
     2041 tatctcattt gataactctg ttcaggttat tagggaaatt atgcaacaaa tattttagta
     2101 caaaagtaac tcattcgtaa agcagtgagt tgcttttttt attccaacaa cctcaaacat
     2161 gtgataaaaa tttcaactac tcttatctat aataaagcta cctgttcttt gaaaattgaa
     2221 tccactccgt cttgattagc gtgcctgtgt aagtagggaa tgagagtatt tccctgtgaa
     2281 gggcaactgg cacaagacgt gtgtgagaat tttctaacag acacggagtt tatcgtttcc
     2341 agactcaaac gatgcgacaa actagataaa ggagttaatc atgaaggtag taaacttgta
     2401 tgatttgaaa caaatgggaa ataaaggtgg ttgtaccatc caattgattc atcactttcc
     2461 ttttggtatg ggcttaggac atctcaaaaa agactacatt gaatttaaac gtgttggtat
     2521 ctttgatggg aaagcagtag aagttactct tcgagaacct tattcgagag atctactgca
     2581 ggttgtcaag tcaataaagt aactgacacc acgagtataa ttattagaaa cgtggaatct
     2641 tattaaattt tataataaat atattaaatg aaaaattatt agatataaac ttatgtaata
     2701 aaacattgag gaaataataa aaatctatgg gtaatgttct gaaagtactt ttaggttagg
     2761 agagaaatat aatgattttt gaaaattgat tcaaaaaata gcgaattgta gaaaacgctt
     2821 aacaaatggt gtataataga cttccagcga aacaaaatat gctacaataa acctatgaat
     2881 tataaagcaa ccttatactc atctgtcttc acaaagcatt ctagtccatc tccgattaac
     2941 gatggacttt atcatctcct tctccagtcc ttgtatgaca tcttccaaag ttcgaaaggc
     3001 tttattctta aatccacgtt tacgaatctc tttccacact tattcaatgg ggttcatttc
     3061 tggtgtgtat ggaggaataa atgcaaagcc aatattagtc ggaatcttta agg
//
LOCUS       DAVBDG010000116         3107 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBDG010000116 DAVBDG010000000
VERSION     DAVBDG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.118"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..177
                     /locus_tag="VQ526_002046"
     CDS             <1..177
                     /locus_tag="VQ526_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2555705.1"
                     /translation="KLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSC
                     NTFYEAFLSCSCFN"
     gene            complement(212..1009)
                     /locus_tag="VQ526_002047"
                     /pseudo
     CDS             complement(212..1009)
                     /locus_tag="VQ526_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1274..1510
                     /locus_tag="VQ526_002048"
     CDS             1274..1510
                     /locus_tag="VQ526_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2555706.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            1741..3027
                     /locus_tag="VQ526_002049"
     CDS             1741..3027
                     /locus_tag="VQ526_002049"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2555707.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT          910 a          447 c          677 g         1073 t
ORIGIN      
        1 aagttagaac ttttatgcga agagtttggg cataaacttt tacctcttcc tccctactca
       61 cctgagtaca atcctattga gaaaacatgg gctcatatca aaaagcacct caaaaaggta
      121 ttaccaagtt gcaatacctt ttacgaggct tttttatcct gctcttgttt caattgacta
      181 tattagaggc gagacatttt tcggttcttt gtcaactgta gtgggttgaa gaaagcgaag
      241 atctagaaag gacaaatttc gtcctttctt ttttgaagtt ttcaaagttc ctaaaaccaa
      301 aggcattgtg cttgataagt ttgatgagat tattggtggc ttccagtttg gcgttggaat
      361 aaggtaattg aagggcgttg acgattttct ctttatcttt gaggaaggtt ttaaacagag
      421 tctgaaaaag aggtggaaaa gcaagagctg atagagatta tagtggtgtt tcaagtcttc
      481 ggaatagctc aaaagtttat ctagaatttc tttattagtc aagtgcatac gaaaagtagg
      541 gcgataaaat cgtttatcac tcagtttctg actatcttgt tgaatgagct ttcagtatcg
      601 cttgatagcc ttgtattcat gggatttcgg atgatggctt gtgttctgct ctcaagaaca
      661 gttatgatat tgagcttatc aaagtcttga gcaataaagc tcatttccat ctcccgattg
      721 aaacagtcac tccccggact gtttcaacgt cctaggacat aatctcagga agacgcgaaa
      781 aatcatgctc aaagtgaaaa tcattgttct tgcgaatgac agttgaagtt gaaatagaca
      841 actgatgatc aatgtcggtc atagaagtct ttttaattag cttctgagca atcttttggt
      901 tgatgataca aggaatttga tgattcttct tgacgataga agtttcagcg accatcattt
      961 ttgaacagtg atagcactta aaacggcctt ttctaagaag aattctagtt tgaatttttt
     1021 atactagaaa atcagaacca taatacctat ataaaaatat tatagttcta ataggattta
     1081 cccaaaagtt ttaaggcggt ctttttagaa ctttaatcgt ttgaaattta ggtagcaaat
     1141 ttgtttctat tttgtcaact tttcctattt ttatcttgtt gaggctggta ttttaacaat
     1201 tcaggaattg atagtgaatg tgtaaaattt tttgttagaa taagtttata aaaaagaaaa
     1261 ggagtatttg attatgttac aaaaaattta tgagcagatg gctaatttct atgatagtat
     1321 tgaagaagag tatggtccta catttggtga taattttgac tgggaacatg ttcattttaa
     1381 atttttaatt tattatttag tgagatatgg cattggttgt cgtagggatt ttatcgttta
     1441 ccattatcgt gttgcttatc gtttgtatct tgaaaaattg gtaatgaatc ggggttttat
     1501 ttcttgttga ggtaatttta gtaaatttcc gaactaattt actcttttat ggaaagataa
     1561 tagtaaatag ctagtaattt ttctaaatca ttttttaata gttggaaata gcaaatcttt
     1621 ctattgtttc ttcttgataa aaaggcgatt ttttattata ataaattgta agatataatt
     1681 gcaggtgaga gtcctgccat gtatgtgaga aaggaagagc ctgagggctc agacaaaatt
     1741 atgacttcag ttgttgttgt aggtacccaa tggggtgatg aaggtaaagg gaagattaca
     1801 gacttccttt cagcgaatgc agaagtgatt gcacgttacc aaggtggtga taatgctggt
     1861 cacacgattg tgattgacgg taagaaattt aagttgcact tgattccatc tgggattttc
     1921 ttccctgaaa aaatatctgt cattgggaat ggtatggttg taaatcctaa atctcttgta
     1981 aaagagttga gctatcttca tgaggaaggt gtaacaactg ataacttacg tatttctgat
     2041 cgtgcgcatg ttattttgcc ttatcatatc gagttggatc gcttgcaaga agaagctaag
     2101 ggcgacaata agattggtac gacaattaag ggaattggtc cagcttatat ggacaaggct
     2161 gctcgtgttg gaattcgtat tgcagatctt ttagataaag atattttccg tgagcgttta
     2221 gaacgtaacc ttgctgaaaa gaatcgtctt tttgaaaaat tgtatgacag taaagcgatt
     2281 gttttcgatg atatttttga agaatattac gaatatggtc aacaaatcaa gaaatacgtg
     2341 atagatacat ctgttatctt gaatgatgcg cttgataatg gcaaacgtgt gctttttgaa
     2401 ggtgcacaag gtgttatgct agatatcgac caaggtactt atccatttgt tacgtcatca
     2461 aaccctgtag ctggtggtgt gacaattggt tctggtgtcg gtccaagcaa gattgacaag
     2521 gttgtaggtg tatgtaaagc ttatacgagt cgtgtaggag atggtccttt cccaactgag
     2581 ttgtttgatg aagtgggaga acgtatccgt gaagtgggtc atgaatatgg tacaacaact
     2641 ggtcgtccac gtcgtgtagg ttggtttgac tcagttgtga tgcgtcatag ccgtcgtgtt
     2701 tctggtatta ctaacctttc tttgaactct attgatgttt tgagcggttt ggatactgtg
     2761 aaaatctgtg tggcctatga tcttgacggt caacgtattg actactatcc agctagtctt
     2821 gagcaattga aacgttgcaa gcctatctat gaagagttgc caggttggtc agaagatatt
     2881 accggagttc gcaatttgga agatcttcct gagaatgcgc gtaactatgt tcgtcgtgtg
     2941 agtgaattgg ttggcgttcg tatttctact ttctcagtag gtcctggtcg tgaacaaaca
     3001 aatattttag aaagtgtttg gtcctaagag atttttaaga tttgtttaag ataggtcggg
     3061 tatactatag acagttataa gaagacctcc taacttgttg taacaaa
//
LOCUS       DAVBDG010000117         2914 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBDG010000117 DAVBDG010000000
VERSION     DAVBDG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.119"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(64..1005)
                     /locus_tag="VQ526_002050"
     CDS             complement(64..1005)
                     /locus_tag="VQ526_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2555708.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(983..2746)
                     /gene="aspS"
                     /locus_tag="VQ526_002051"
     CDS             complement(983..2746)
                     /gene="aspS"
                     /locus_tag="VQ526_002051"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2555709.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVVNATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EGTAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          847 a          691 c          579 g          797 t
ORIGIN      
        1 aaccatatat acggtaaggc gacgctgacg tggtttgaag agattttcga agagtattaa
       61 tcactaatta tctatctcaa caaatcttcc tagaatatga acattttccg agacagagac
      121 aaaggagctt ggatccactt gtgtcataat ctgtttaaat tcattaaact ctgcacgtgt
      181 aatgacagtg attaaaactg cctttctctc gtgattatag gttccttctg catcatggat
      241 catggttgct ccgcggtgca attttttatg gattttttca attaccttct ctggatgatt
      301 tgtcacaatc atggcctgca tacgcttttg cttagtaaag actgcgtctg tcacacgact
      361 agagacaaag atggtaatca tagaataaag agcgtatttc caaccaaagg tcaaacctgc
      421 tatcagcatg atagttccat ttaccaagaa agaaatacta ccgacattct tacccgtttt
      481 cttgcgaata gtcaggctga cgatatccgt cccaccactg gagatattgt ttcgaagagc
      541 aaaaccaatc cccaaaccca taacaacacc cccaaaaagg gcattgataa tgggatcctc
      601 cgtcaaggtt gccacaggga caaactggat aaagaaggaa ctcatagata ccgtgataaa
      661 ggtaaagacg gtgaacttat ggccaatctg ataccaagct aagaccatca aagggaagtt
      721 aatggcgtaa aagcttagcg aaatcggaat atgaaaacca aaccagtgat tactcaaggc
      781 agagataatc tgtgccagac ctgttgcacc actcgaatac acatgccctg gttggaaaaa
      841 gaaattaact gctactgctg ataaaaaacc atagaccaga gaggccgaaa tcttctcatc
      901 atacttttct cgagagatac tttgtaagac acgtaaaatt tttatctgat aagcaaagcg
      961 gcgcagataa tagcgccacc gcttaattcg ttttgtttgt ttcatcttct tctacttgta
     1021 agctgagttc ctctagttgt ttgagagcga ctgttgatgg agcttgtgtc attgggtcag
     1081 ttgccttgtt gttcttagga aaggcaatga cttcacggat attttcttct ccagcaagca
     1141 acatgacaaa acggtcaagc ccgatagcca aaccaccgtg tggtgggaaa ccatagtcca
     1201 tggcttcaag aaggaaacca aactggtcat tggcttcttc agttgagaaa ccaagagcct
     1261 tgaacatgcg ttcttgaagg tctttttggt tgatacgaag gctaccacca ccaagctcat
     1321 aaccgttcaa gacgatatcg taagcaatgg cacgaacctt agccaaatca ccttctaatt
     1381 cgtgagcagt cccttcctgt ggaagtgtga atggatggtg ggcgctcatg tagcggcctt
     1441 cttcttcaga ccattcaaac atcggccagt caaccaccca aaggaagttg aacttatcat
     1501 tatcaatcaa gccaagctct ttagcaatac gtccacgaag ggcacccagt gttgcattaa
     1561 ccacttcaag cgtatccgcc acaaagagaa ccaagtcctt atcttcaaga gcaagcgctg
     1621 ttgtcaattc ttcttggata ccagtcaaga acttggcaac tggtccgttt aattctccat
     1681 caaccacctt gacccaagca agacctttgg caccatactg tttggctact tccgtcatct
     1741 tgtcgatgtc tttacgtgaa tagttgtccg cagctccttt gaccacaatc gcttttacag
     1801 caggtgcttc tgaaaagact ttaaagtcta cacctttgac cacttctgtc aagtcctgaa
     1861 gcaacatgtc aaaacgagta tctggcttgt cagaaccgta aagagccata gcatcatcgt
     1921 atttcatacg agggaacggt agcgttactt cgatgccttt tgtttccttc atcacgcgcg
     1981 cgatcaagcc ttctgtaata tcttggattt ctttctcagt aaggaaggac gtttccaagt
     2041 cgacctgagt aaattcaggc tggcggtctc cacgcaagtc ctcgtcacgg aaacatttaa
     2101 cgatttggta gtaacggtca aaaccagcat tcatcaagag ctgtttcgtg atttgtggac
     2161 tttgaggaag agcgtaaaaa tgccccttat taacacgaga cggcactaaa taatcacgcg
     2221 ccccttcagg cgttgactta gaaaggaatg gtgtctccac gtcgataaac tccaactcat
     2281 ccaaatagtt gcggatagag tgggtcacct tggcacgaag tttaagattt tccaacattt
     2341 ctggacgacg aaggtcaagg taacggtaac gcaaacgtgt atcgtcattt gcctcaatgc
     2401 catccttaat ctcaaatggt gttgtcttag ctgtgttaag cacaataaga gctgtcacgt
     2461 ttaactcaac cgcaccagtt ggcaacttat cattggcttg ctcacgcgca gcgacctgac
     2521 cagtcacctc aataacaaat tcgctacgaa ggctttcagc tgttgccata acctctgcag
     2581 atactttttc agggttgata accaactgca tgattccttc acggtcacga agatcgataa
     2641 agatcaaacc accaaggtca cgacgacggc caacccatcc tttcaaggtt atttcttgtc
     2701 cgatgtgttc ctcacgaaca cgaccagcat acatactacg tttcattatt tctctcctct
     2761 tttattctgt tactatttta ccataaaagc gcaggctctt catgaaaatc atcagaaaag
     2821 tttgccagtc tttaaaagtc aggtgaaagc cctaaaaatt agcgctaatg ctcttcgaaa
     2881 atctcttcaa accacgtcag cgtcgcctta ccgt
//
LOCUS       DAVBDG010000118         2891 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBDG010000118 DAVBDG010000000
VERSION     DAVBDG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.120"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            525..896
                     /locus_tag="VQ526_002052"
     CDS             525..896
                     /locus_tag="VQ526_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555710.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQIIAYI
                     GAILCLTIMLLLIWGLKYYKK"
     gene            1525..1908
                     /locus_tag="VQ526_002053"
     CDS             1525..1908
                     /locus_tag="VQ526_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2555711.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            1960..2649
                     /locus_tag="VQ526_002054"
     CDS             1960..2649
                     /locus_tag="VQ526_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2555712.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            2691..>2891
                     /locus_tag="VQ526_002055"
     CDS             2691..>2891
                     /locus_tag="VQ526_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555713.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWL"
BASE COUNT          819 a          435 c          530 g         1107 t
ORIGIN      
        1 ttaattagaa aaatattttc aaaatcttcc aaaaaggata cggatagtac tctctaaaaa
       61 ataaatttct cccctgtatt tgctagctta atttacaggg gagtttcttt aatattgttt
      121 cgcctgtcaa aatgatatgt gatagcagga tgaaaagtct acaaacaagt tggtatcaat
      181 acaatataaa gaggaaagta tgtttttcat gcttgctttt ttaattttta caattcaaga
      241 ggttttgatg accatttatg atttgagtga tccaaggtca aaataagtgc tatactagca
      301 gtgtaaatgt tcttgctcaa ctaagataaa tcgcacattt aggaggaaat cttatgaaga
      361 aaaaaatact gattattttc gttctgtatc tgatcatgtc catctttctt tatccgctta
      421 gggagagtat ttggtataat ctattttata ccatagccta tatgattgcg gttatgatct
      481 atttttcttt aattaaaaag aaagaaaaga aatgaaagat tttcttgatt aaaaagaatc
      541 atattctttg tgagattgtt cctgggtcag ttgcctttgc ttgtttctac ttatctattt
      601 ctatttcgtc agttttccaa tttcttttag tggttattaa cttagcttct attttggtca
      661 ctgtttacct cactagggaa atgcggataa aagagtttga agatgatgat ttggttagtc
      721 ctagaaccaa tcaacttatg ttcatcggct tgacaggctt tatgtctatt atttgtttgt
      781 atagagggat cacagcagga gaatcctacc aacaaataat cgcttatatt ggcgctattc
      841 tctgcttgac catcatgctt ctgctcattt ggggattgaa gtattataaa aagtagaggc
      901 taatgcagtt tttgcaattt aagatgtctt gatgacaatt caagatttgg atgaaaaact
      961 ttgacaaata gtgctatact gaaattgtca aagttgcaac gggtgctgct acattatgtt
     1021 aaaaatcgct cttgattgat aagttggatt tgttaggaaa ggagtaaata attatggata
     1081 agaaaaagat agtttcgact ataatatgta tagtatttct tgtagtgtca gtagataatt
     1141 tttttagaga cttaaccccg ttactgttca ttttaaatat tattggcctg tcttgctttt
     1201 cagtgctaac atacattaac ataaaagaga tactcttaaa tataagtaaa tgatgatttg
     1261 gttagtccta gaaccaatca acttatgttt atcggcttgg cagattttat gtttgtcatt
     1321 tgtttgtata gaggtatttc agaaacagaa ttctatcaac aactaatcgc ttatataggt
     1381 gtattctctg cttgtttatc acgcttctgc tcatgtgggg cttgaagtat tataaaaagt
     1441 agctttctat ccttttctga caggatattt gagtagaagg ctctattaaa ttgttagtga
     1501 aataaatgaa tggaggtatt taatatgaaa tacagattat tttttgttat tttcttgagt
     1561 agtgtgttgg atattctttt agggacattt ttacaaatct ctatcgtatc tattgggtgg
     1621 cttgttcttt acagtggttt gtttgaagca ggagttttcc ttcttgctaa taaaggggtg
     1681 gcggtaaaaa tcaaggaagt agatattcga aatcgcttta aatttatttt tggaaaaacc
     1741 ttatggtttc aaattctttt gctcatcttt ttgataatca aactttatct tggtttggat
     1801 gcgaggttga ttttattcta tggacatatt ttcattgtct ttaatgcctt aatgtatcta
     1861 ttatctagta gtcaggttag ccttaaaaaa aacaaactgt cttcttaatc ttacagcagt
     1921 agagcctgac aaggtgaggt catagtaggg aggatttcta tgaaaaagta tcaacttcta
     1981 ttcaaaataa gtgcagtttt ctcttactta tttttcgtat ttggtctttc tcagatgacg
     2041 cttattattc aaaattattg gcaattttct tcccagattg gcaatttcgt ctggattcaa
     2101 aatttcttga gtttgctatt tagcggagtc atgatttgga ttctggttaa gacaggtcat
     2161 ggttatctct ttcacattcc aagaaaaaaa tggctttggt attcgatttt gacagtatta
     2221 gtggtagtgc tccagatctc ttttaacgtt cagacagcta aacatgttca gtcaactgct
     2281 gaaggttggg ctgtattgat cggttatagt gggaccaact ttgctgagct aggtatctat
     2341 ataactttgt tctttctgac tccacttatg gaagagctaa tctatagagg attactgcaa
     2401 cacgcctttt ttaagcattc gagatttggc cttgatttgc ttcttccgtc aattttattt
     2461 gctcttcctc atttttcaag cctgcctagt ttgttagata tcttcatctt tgcaacatct
     2521 ggcatcatct ttgctagttt gacccgctat accaagagca tttatccttc ctatgcggtg
     2581 catgtgatca ataatatttt cgcaacatta ccatttttgc tgactttttt acatagggtc
     2641 ttggggtaaa aaaacaaaag gcttgctttt cagccataga ggagattatc atgtataaac
     2701 acttattttt cctagattcc aaaactttag atcggttgac accctatatt ctagtcttgg
     2761 cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg gtggttttta
     2821 atttcctaaa ttccatgcta gctttaatgg ctatattcat aggggctggc tatgtggtcg
     2881 gattttggtt a
//
LOCUS       DAVBDG010000119         2806 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBDG010000119 DAVBDG010000000
VERSION     DAVBDG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.121"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            61..777
                     /locus_tag="VQ526_002056"
     CDS             61..777
                     /locus_tag="VQ526_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2555714.1"
                     /translation="MKKRYLVVTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            1086..1511
                     /gene="rplK"
                     /locus_tag="VQ526_002057"
     CDS             1086..1511
                     /gene="rplK"
                     /locus_tag="VQ526_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2555715.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            1719..2408
                     /gene="rplA"
                     /locus_tag="VQ526_002058"
     CDS             1719..2408
                     /gene="rplA"
                     /locus_tag="VQ526_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2555716.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT          939 a          529 c          634 g          704 t
ORIGIN      
        1 aagattagtc aaacgtttat catcagaagt agataaacta ttagaaataa aggagtagaa
       61 atgaagaaaa gataccttgt cgtgacagcc ttgctagcct tgagtctagc agcttgttca
      121 caagaaaaag caaaaaatga agatggcaca gctaagacag aacagacagc caaagctgat
      181 gggacagtcg gcagtaagtc tcaaggggct gcccagaaaa aagcagaagt ggtcaataaa
      241 ggtgattact acagcatcca aggaaaatac gacgaaatca tcgtagccaa taaacattat
      301 cctttgtcga aagactataa tccgggggaa aatccaacag ccaaggctga gttggtcaaa
      361 ctcatcaaag cgatgcaaga ggcaggtttc cctattagtg atcattacag tggttttaga
      421 agttatgaaa ctcagaccaa gctctatcaa gattatgtca accaagatgg aaaggcagca
      481 gctgaccgtt actctgcccg tcctggctat agcgaacacc agacaggctt ggcctttgat
      541 gtgattggga ctgatggtga tttggtgaca gaagaaaaag cagcccaatg gctcttggat
      601 catgcagctg attatggctt tgttgtccgt tatctcaaag gcaaggaaaa ggaaacaggc
      661 tatatggctg aggaatggca cctgcgttat gtaggaaaag aagctaaaga aattgctgca
      721 agtggtctca gtttggaaga atactatggc tttgaaggcg gagactacgt cgattaatac
      781 tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac ctactgactg cgtcggttct
      841 attcacaacc tcaaaacagt gttttgagct gacttcgtca gttttatctg caatctcaaa
      901 gctgtacttt gagcagccta cagctagctt cctagtttgc tctttgattt tcattgagta
      961 taaaaaataa acttttctct tgcaattcca gataaatagt gtataatgga tgggtatgtg
     1021 aaaaacatac ttgtgggagg taaaaatctc taattaccgc caaaaccaca aaggaggatt
     1081 taaaaatggc taaaaaagtc gaaaaacttg taaaattgca aatccctgct ggtaaagcta
     1141 caccagctcc accggttgga cctgctcttg gtcaagctgg tatcaacatc atgggattca
     1201 caaaagagtt caacgctcgt acagctgacc aagctggtat gatcattcca gttgttatct
     1261 cagtttacga agataaatca tttactttcg ttacgaaaac accaccagct gctgttcttt
     1321 tgaaaaaagc tgcaggtgtt gaaaaaggat caggtacacc taataaaact aaagttgcta
     1381 cagttactcg tgcacaagta caagaaattg cagaaactaa gatgccagat ttgaacgcag
     1441 caaacgtaga gtctgcaatg cgtatgatcg aaggtactgc tcgttctatg ggattcactg
     1501 ttgttgacta atcaataaca cagtagaaaa tctcacagca gtctattagt tgcttttcat
     1561 actaggcaag tgactgaggc ttgtacttgg gtacagcaag ggagcttaag gccgtaggaa
     1621 gagaaaaata gtagactgaa aacccgcaag acttcatcat ttcgagaagt gacgtgggag
     1681 atgaaaatcg attgaaccac ttacaaggag aatagaaaat ggctaaaaaa agcaaacaac
     1741 ttcgtgctgc tcttgagaaa atcgacagca caaaagcata cagcgtagaa gaagctgtag
     1801 cacttgcaaa agaaactaac tttgcaaaat ttgacgcaac tgtagaagtt gcttacaact
     1861 tgaacatcga cgttaaaaaa gctgaccaac aaatccgtgg agcaatggta ttgccaaacg
     1921 gtactggtaa aacttcacgt gttcttgttt tcgcacgtgg tgcaaaagct gaagaagcaa
     1981 aagctgctgg tgcagacttt gttggtgaag atgaccttgt tgctaaaatc aacgacggtt
     2041 ggttggattt cgacgtagtt atcgctacac ctgatatgat ggctcttgtt ggacgtcttg
     2101 gacgtgtcct tggaccacgt aacttgatgc caaaccctaa aactggtact gtaacaatgg
     2161 atgttgctaa agcagttgaa gagtctaaag gtggtaaaat cacttaccgt gctgaccgtg
     2221 caggtaacgt tcaagcaatc atcggtaaag tatcatttga agctgaaaaa ttggttgaaa
     2281 actttaaagc tttcaacgaa acaatccaaa aagcaaaacc agctacagct aaaggaactt
     2341 acgtaacaaa cttgactatc acaactactc aaggtgttgg tatcaaagtt gacgtaaact
     2401 cactttaatc agtagttaaa gtaatgtaaa aaagttgaag acgctatgtc tcaacttttt
     2461 ttgatatacg acgggcatgt cgtatatctg aggtgtaaat cctcggtggg cacctactac
     2521 tggtgaaccc aatagcgatt cccaagtctg actatcgtga ggtagcggat taaaacggtc
     2581 tggggataga ccgttttaag tctgacgctg gaaataagaa tcgtcagagg aagggatagc
     2641 gaaatcgtgg ctctacgaac aggaacgtga taataaggcc tatatagcgg ataaggtatc
     2701 tggctggggc gaccactatc aattatagta aaatgaaata agagtaggac gaatcgatca
     2761 gggcagccaa atcgatttct aacaatgttt tagaagaaga gatgta
//
LOCUS       DAVBDG010000120         2804 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBDG010000120 DAVBDG010000000
VERSION     DAVBDG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.122"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(304..1614)
                     /locus_tag="VQ526_002059"
     CDS             complement(304..1614)
                     /locus_tag="VQ526_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2555717.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1851..2723
                     /locus_tag="VQ526_002060"
     CDS             1851..2723
                     /locus_tag="VQ526_002060"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2555718.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          839 a          486 c          654 g          825 t
ORIGIN      
        1 tcctgatcta tttgtcctgt tcttatttca tttcactata tctcaaattg agtatgacga
       61 agtgcgctcc catgtcctgg gaacgcactt tcttcatatt tttcatattc ttgaatccat
      121 cgataaagac tattgggatg aatttttaaa gttgaactaa tcatttttac aggatgagat
      181 ttacagcaga gagtttgaag gctttatcaa aggtttttct tggcataatg acttttcctc
      241 gtttccactt aattttgtgt ctactttatt ataccaagtc cacccttaag ttagataata
      301 aatctaactt aaggaagcta gaaggatgag aatccaggtg gtcaagagtc ccaaacttaa
      361 gctgatgggg acacccagaa taatttgctt tttgaaggca aggccacgtt cctctatatt
      421 gggaagtgag agttgaatga gagaaccagc tgatgaaaag ggtgagatat tagtagatag
      481 agcgccaata acggtggctg ttgtgagtaa gtgaatatca atctgaggat tttgagcact
      541 gatgatagca atgatgggaa agagggctgg agctacaacg gatagggtgg aactaaagag
      601 tgacatcact ccggctatca cacaaaagaa cagaggtaac cagaaatgag gaatggttgt
      661 tgtcatgagg tgccctatca gtgtgactaa acctgacttg accgctagag acattagtaa
      721 gctcatgccg cagagcatga taattgtagc ccagggaacc ttagctaaaa tggcttcttg
      781 cttccctaat ttgagcctta aggcgaggca gaccatgagt attgagacaa agccaatatc
      841 aaatgttttt tgataagtag ctatccaggc gatgtttggg aaaatgagat gcaacaaggg
      901 aaaaagccaa accaaaacca tgctgctgat catgagcaag gtggtttgtc tttgaacctt
      961 gctgaggagt ggtggttggt caatagtcaa ggatgagttt gttcttccct tactatagtg
     1021 actgtaacag gataataaaa gcaagacgat gagtgggtag ataatgctga cgataaagat
     1081 atgattgcca agtgaaaaag cttgctcttc ccatcccatt tgcttaaaca ggccttgaaa
     1141 gacaatgcct gagctactgg ttatcaaatt agcccctcct gaagctcccc aattgacggc
     1201 ttgagctcca atcaaagggt gtttgtccgc tttttgacag agggtaatcg ctagaggaca
     1261 gcaaacggcc atagtagtga aaaatccagc acctaaagca gacaaaaggg ttgccatcag
     1321 gtataaaatc atgtagaggg cgttagggtg ggtgcgtgtg cggtagagaa tgtgttgagc
     1381 caaaacatca agagtaccgt tagttgttgc aacgttataa aagagagaga cgctaaaaat
     1441 ggtaaaaaag agtgaggttg gccaaaaatg aagaagttct ttggggctta atcccatgag
     1501 agtggttgcg atgaggtaag aaaaagcaat agccagcagg ccaatattga ttttggtgcg
     1561 gtaaccaatt ccaatggcta gagcaatggc gctaatcatt attaaatgaa tcattgaatt
     1621 gtcctttcag ttagaatatg gaaataggtt ggcaattttg gcgctcaaaa gaccacctgt
     1681 ttcctattgg tacttgacta ttatacacaa aaaaaataca attgtcttga caattgtatt
     1741 gacacttgtt agaattaaaa atgaggtatt caggcttgtg atttcctatg gaagttaata
     1801 gtaattgcct ctagtgctta caagtgatat taaaaataga ggacttagtg atgtcaatca
     1861 tttcaactga tttaacccct tttcaaatag atgatacatt gaaagcagcc ttgcgagaag
     1921 atgttcattc cgaagattac agtaccaatg ccatttttga tcatcatggc caagccaagg
     1981 tgtcgctttt tgccaaggaa gctggtgttt tagcgggact aaccgttttt caaagggttt
     2041 ttaccctatt tgatgccgag gtgaccttcc agaatcctca tcaatttaag gatggggatc
     2101 gtttgactag tggcgatttg gttttagaaa tcataggctc ggtgagaagt ctcttaacat
     2161 gtgaacgcgt tgccttgaat tttttacaac atttatcagg gattgcttcg atgacagctg
     2221 cttatgtaga agccttaggc gatgattgca ttaaggtatt tgatactcga aaaactactc
     2281 ctaatttacg tctttttgag aaatatgccg tgagagttgg cggtggctat aatcatcgct
     2341 ttaatttatc agatgctatc atgctaaaag acaatcacat tgcggcagta ggtagtgttc
     2401 agaaggcaat tgctcaagcg cgtgcctatg ccccttttgt gaaaatggtc gaggtggaag
     2461 tggaaagcct tgctgctgcc gaagaagctg cggcggcggg tgctgatatt atcatgttgg
     2521 ataatatgtc attggaacag attgaacagg ccattaccct aattgcagga cgttctcgga
     2581 ttgaatgttc tggaaatatt gatatgacca ctattagccg ttttcgtggt ttagcgattg
     2641 attacgtctc cagtggtagt ttaacccata gtgctaagag tcttgatttt tccatgaagg
     2701 gtttaaccta ccttgatgtc taagttgtaa aataaactaa ctttttaaag gatgtctttc
     2761 ctctagaacg agttttatgt cagatagttt aaacgcctct tcaa
//
LOCUS       DAVBDG010000121         2709 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBDG010000121 DAVBDG010000000
VERSION     DAVBDG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.123"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(482..707)
                     /locus_tag="VQ526_002061"
                     /pseudo
     CDS             complement(482..707)
                     /locus_tag="VQ526_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1555..2427
                     /locus_tag="VQ526_002062"
     CDS             1555..2427
                     /locus_tag="VQ526_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2555719.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(2573..2709)
                     /locus_tag="VQ526_002063"
                     /pseudo
     CDS             complement(2573..2709)
                     /locus_tag="VQ526_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          807 a          474 c          524 g          904 t
ORIGIN      
        1 aaataagata tgtacaaatt gattagaaag tcaaatgaat ttctacaaat gttttagtaa
       61 tcgcaatcta cttgtctaga ttcgatctga tatattttcg atttaatgat atggtattta
      121 aaacctccaa agtagcttgc tccattcttt tacttacgtg agtgtagatg ttatttactg
      181 ttttagcgtt tttgtgttcc actctaacca ttatagcatt cttctcagct agtgtactaa
      241 ggagtgtgtg cctgaaaata tgggaactaa ggggctggtt tatcggtttc tctagtttag
      301 tatttgcctt ttgcaaagtg atgttaaatg cctttctcta aattcacata tcactattgt
      361 ttaacaaaat ctaatctact ttaggtcact tattcttttt ttgaaatgta gaatgaactt
      421 tttcaaagtt tttcgaatct tttaaaatct gtttgtttta tatcgccatt ctcccccttt
      481 tttaattctc cctatatagc ctgatagctt tcccgatggt acgaatatgg ttgctttcgt
      541 ctaggtggat gtcggggtat tcgggattga gtttttttga ggcagccttg gcggagtttc
      601 ttgacatagt tagtgccgtc tacttggaag atgccgatgg tattatagtc aatctgtggg
      661 gtattcttga taaataggta gtcgctgttt cttatctttg gctccatgga cttgctgacg
      721 acataagcga ttgggtcgta gtcgtctggg ataatggaaa ctccataact aaatcgtcgt
      781 cctgcatcga gcggctacct gcagagataa actacctaac acgagagtaa gtagtctgtc
      841 tgtagtcgtc cagtctgatg atttttacga tacttcgttt ttctgatcat acagttgcct
      901 ctcggcatag gtcagaactt taccttgtct gggtggtgcc cgttggtcgt agatagattg
      961 gatatcgcta ggagaatcct tttgaactgg aggaaagagg gcatcaatca agctactgaa
     1021 tactttaact aagtcaaata tagtattttt cttagtagac ctaacccttt tttcataatt
     1081 tctaatggtg tttttactta tacctatctt agtacccaat tcttattgag tccaaccatt
     1141 actagtctat attgttttat agttgattga gtttggaata gtacgctgta gctgctaaaa
     1201 catttctagg aattaatttg cctttcctaa tagagttgtt catatcttat ttcaatctat
     1261 tatatttttc gcctctaaca atcgcaatct cttctttatc tacgaacgaa atcgctttct
     1321 atttttgtaa gtaaagcatc acacgaaatc cacgaaaatg aaaacctttg ttgtgttttc
     1381 gtaaaaaatt tgttgacaga gcacgaaacg cctactataa ttaaatcaag tttaaggaaa
     1441 taacaaaaaa acaaatcgga gggaaagacg atgaacacat taaatgagaa agtaatcaat
     1501 atctgtaaag cagtagttaa agaaacttta atccaagaca tttaggagaa agaaatgaag
     1561 aaatcaagaa aattagccac attaggaatc tgttcagcct tgtttttagg cttggctgct
     1621 tgtcaacaac aacatgctac ttctgagggt acgaatcaaa ggcaaagcag ttcagcgaaa
     1681 gttccatgga aagcttcata caccaaccta aacaaccagg taagtacaga agaggtcaaa
     1741 tctctcttat cagctcactt ggatccaaat agtgttgatg cattttttaa tctcgttaat
     1801 gactataata ccattgtcgg ctcaactggc ttatcaggag atttcacttc ctttactcac
     1861 accgaatacg atgttgagaa aatcagtcat ctctggaatc aaaagaaggg cgatttcgtt
     1921 gggaccaact gccgtatcaa tagttattgt cttttgaaaa attcagtcac cattccaaag
     1981 cttgaaaaga atgaccagtt gcttttccta gataatgatg cgattgataa aggaaaggtc
     2041 tttgattcac aagataagga agagtttgat attctatttt cgagagttcc aactgaggca
     2101 actacagatg tcaaggttca cgctgaaaag atggaaacat tcttctcaca atttcaattc
     2161 aatgaaaaag ctcgaatgct gtctgtagtc ttgcacgaca atctggatgg cgagtatctg
     2221 tttgtaggcc acgttggggt cttagtacct gctgatgacg gtttcttatt tgtagagaaa
     2281 ttgactttcg aagagcccta ccaagcgatt aaatttgcta gtaaggaaga ttgctacaag
     2341 tatttgggca ccaagtatgc ggattataca ggcgagggac tggctaagcc ttttatcatg
     2401 gataatgata agtgggttaa actttaatag tgttatgata tgacagtaat cttaggattt
     2461 ttatttctat ttttgataag ttggtgggtt atttcaatcg ttaatagcca gaaaccacgc
     2521 aaaaatgaaa catttattgg ctatgtccaa agatacgata ttgatgggaa tggctatcaa
     2581 tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat cgtaacacca ctttgaacaa
     2641 gagttacttc aacccattag ctccgacgga ttaagttgct ttcgtgaata ccaaaaacag
     2701 ccgcaattt
//
LOCUS       DAVBDG010000122         2644 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBDG010000122 DAVBDG010000000
VERSION     DAVBDG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.124"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            434..2539
                     /locus_tag="VQ526_002064"
     CDS             434..2539
                     /locus_tag="VQ526_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2555720.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTSEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
BASE COUNT          848 a          477 c          611 g          708 t
ORIGIN      
        1 tcctgttctt atttcatttt aatatatagt tttgagatgg ttgatagaac tgacgaagtc
       61 agctcaaaac atggttttga ggttgtagat aaggagaagc tgatgtggtt tgaagaggtt
      121 ttcgaagagt gtaaaaaaat attggtcagt taaagccaaa actcttgacc ttttctgacc
      181 aatgtagtat attatgaacg taaggtaaat gaaagcctta ctagaacact tatttaaagt
      241 aaaatagaaa gaggtgggtc tatgtttcat ctagaaatct tcagaagtaa agatggtcta
      301 ctcctgcttg aaaaagaaaa accagaaata gtacgtaggg tagcggttta actagtctaa
      361 attttttaaa acaatggtca aagatagtca atatcagtaa tcataactaa gtaaacaaaa
      421 agaggtaaag aatatgaata acaactttaa taattttaat aacatggatg atttatttaa
      481 ccaattgatg ggtggtatgc gaggatatag ttctgaaaat cgccgttact taattaatgg
      541 acgcgaagtc acatctgagg aatttgctca ctatcgtgcg actggtcaat taccaggaaa
      601 tgcagaaact gatgtgcaaa tgccacaaca ggcatcaggt atgaaacaag acggtgtcct
      661 tgcaaaacta ggtcgaaact tgacagcaga agcgcgtgag ggtaagttgg atcctgttat
      721 cggacgaaac aaggaaattc aagaaacatc tgaaatcctc tcacgccgca ccaagaacaa
      781 tcctgttttg gtcggagatg caggtgttgg taagacagca gttgtcgaag gtctagcgca
      841 agccattgtg aacggagatg ttcctgctgc tatcaagaac aaggaaatta tttctattga
      901 tatctcaggt cttgaggctg gtactcaata ccgtggtagc tttgaagaaa atgtccaaaa
      961 cttagtcaat gaagtgaaag aagcagggaa tattatcctc ttctttgatg aaattcacca
     1021 aattcttggt gctggtagca ctggtggaga cagtggttct aaaggacttg cggatattct
     1081 caagccagct ctctctcgtg gagaattgac agtgattggg gcaacaactc aagacgaata
     1141 ccgtaacacc atcttgaaga atgctgctct tgctcgtcgt ttcaacgaag tgaaggtcaa
     1201 tgctccttca gcagagaata cttttaaaat tcttcaaggc attcgtgacc tctatcaaca
     1261 acatcacaat gtcatcttgc cagacgaagt cttgaaagca gcggtggatt attctgttca
     1321 atacattcct caacgtagct tgccagataa ggctattgac cttgtcgatg taacagctgc
     1381 tcacttggcg gctcaacatc cagtaacaga tgtgcatgct gttgaacgag aaatcgaaac
     1441 ggaaaaagac aagcaagaaa aagcagttga agcagaagat tttgaagcag ctctaaacta
     1501 taaaacgcgc attgcagaat tggaaaggaa aatcgaaaac cacacagaag atatgaaagt
     1561 gactgcaagt gtcaacgatg tggctgaatc tgtggaacga atgacaggta tcccagtatc
     1621 gcaaatggga gcttcagata tcgaacgttt gaaagatatg gctcatcgct tgcaagacaa
     1681 ggtgattggt caagataagg ccgtagaagc tgtagctcgt gctatccgtc gtaaccgtgc
     1741 tggttttgat gaaggaaatc gcccaatcgg cagcttcctc tttgtagggt ctactggggt
     1801 tggtaagacg gagcttgcta agcaattggc actcgatatg tttggaaccc aggatgcgat
     1861 tatccgtttg gatatgtctg aatacagtga ccgcacagct gtttctaagc taattggtac
     1921 aacagcaggc tatgtgggtt atgatgacaa tagcaatacc ttaacagaac gtgttcgtcg
     1981 caatccatac tctatcattc tcttggatga aattgaaaag gctgaccctc aagttattac
     2041 ccttctcctc caagttctag atgatggtcg tttgacagat ggtcaaggaa atacagtaaa
     2101 cttcaagaac actgtcatta ttgcgacctc aaatgctgga tttggctatg aagccaactt
     2161 gacagaagat gcggataaac cagaattgat ggaccgtttg aaacccttct tccgtccaga
     2221 attcctcaac cgctttaatg cagtcatcga gttctcacac ttgactaagg aagacctttc
     2281 taagattgta gatttgatgt tggctgaagt taaccaaacc ttggctaaga aagacattga
     2341 cttggtagtc agtcaagcgg ctaaagatta tatcacagaa gaaggttacg acgaagtcat
     2401 gggggttcgt cctctccgtc gagtggttga acaagaaatt cgtgataagg tgacagactt
     2461 ccacttggat catttagatg ctaaacatct ggaagcagat atggaagatg gcgttttggt
     2521 tattcgtgag aaagcctaag acagaatttt gaggataaaa aagaaggagc cagctgaaaa
     2581 aaactggttc ctttctaggt acgacgggca tgtcgtgtag taggttgaaa ctagaatagt
     2641 acac
//
LOCUS       DAVBDG010000123         2624 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBDG010000123 DAVBDG010000000
VERSION     DAVBDG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.125"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            203..517
                     /locus_tag="VQ526_002065"
     CDS             203..517
                     /locus_tag="VQ526_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555721.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            527..733
                     /locus_tag="VQ526_002066"
     CDS             527..733
                     /locus_tag="VQ526_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2555722.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            733..930
                     /locus_tag="VQ526_002067"
     CDS             733..930
                     /locus_tag="VQ526_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555723.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            933..1667
                     /locus_tag="VQ526_002068"
     CDS             933..1667
                     /locus_tag="VQ526_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2555724.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            1679..2320
                     /locus_tag="VQ526_002069"
     CDS             1679..2320
                     /locus_tag="VQ526_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2555725.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2338..2568
                     /locus_tag="VQ526_002070"
     CDS             2338..2568
                     /locus_tag="VQ526_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555726.1"
                     /translation="MKRVILVAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          775 a          428 c          537 g          884 t
ORIGIN      
        1 acggcaaggt gaagctgacg tggtttgaag agattttaga agagtattaa tgatagataa
       61 agtggtcagg aacttactcc tgaccttttt cttttgcaaa atgacaaaaa tcataaactt
      121 tttgacaact ataattgtca aaaagaaaaa gatatgttac aatgaattca agttaagaaa
      181 taggaagcag aaaggagtta taatgtgggt actaggattt atactattta tgattttctt
      241 ttattctaat aattctaaaa aaatcaagaa actagagaat aaaatcaaaa aacttgagcg
      301 aaaagagaaa ggaaacgcag aaatgtcgag attattacaa gaaatgattg gaaaggaacc
      361 aattataacg ggagtgtata ttgggccaga taactgggaa gttgtggatg ttgatgagga
      421 atgggtcaaa ctacgtagtg ttgataatac gggaaaagaa aaattcaagt tgcaacgtat
      481 tgaagatatc caaaccgttg aatttgacgg agagtaggtg tgtaacgtgc aactaaaaaa
      541 tcgtctaaaa gagcttcgag ctcgcgatgg tctcaatcaa accgacctag ccaaactggc
      601 aggggtttcc agacagacca ttagcctact agaacgggat gagtacaccc catccattat
      661 cattgccctg aaaatatctc aaattttcaa cgaaacagtc gaatcggtat ttcgtttgga
      721 ggaggacgag tgatgaacaa gtataaagtg atttattatg tagtggtcat agctttatta
      781 gtcagcgtat ttctactgat tgggatggac ctaagctggt ttaatcccta tcaaagcgac
      841 caatttgttt gggtctactt tgctctcatc ccagtaactg aatggattga aaagaaatcc
      901 aaaaatctag caagtgaaaa aggagaatga atatgaaaaa gaaacgtaga ttgttatttt
      961 tgatgtctat tgtcttgggg gggttcttgg gcatgtttgt agggatgttt aaggcacgtg
     1021 tcgaatccca cgaaattatt ttagatgtaa aagccttgat gccatggata tcagctattt
     1081 gtttactgat aggtttcatt agtatgtttt tgactttcaa tttcttaaag aaaagcagaa
     1141 aatttcattc cttgtatcaa gaggaaatgg atgacgatct gaatgaaacc tattatgtgc
     1201 aaatgtatcg gaatcttgag tttggaacca ttgcttttaa tattacaggt gtagcgattc
     1261 cattggctat ttttatttca ttaagtgagg tgattatatt gcatacaaac cctcaaacat
     1321 ttttcctttc tttcttactc tttgtggtat tcttagtcgc tcaaaaatct ctttttaaaa
     1381 ccattgcgat tgttcgtcag tttgatttgg aatttttcgc tacaccaaag gatgtcttga
     1441 actatataaa ttcttatgat gaaggggagc gtcaggctaa tttggaacag agttttcgaa
     1501 ttttattcca attacaccaa tatgtcttac cagccttata tatttttctt attatcattt
     1561 ctttcttgac aggagagatt cagttactag ctttcttgct tgtaggagcc atccatgttt
     1621 atatcaatgt gatgcagtta cctatggtaa aacgttattt caaataaagg agttatctat
     1681 gaaattactt aaaaaccttg gctggattct tctagccctt ctatcctttt tatttatcta
     1741 tggctttatt caggggctcg cgactgcgtc gcttgcttta ggcgcttcac cctatgctgt
     1801 tactctactc tatgtggcct tggctggagt atatgtgtac ggcatttaca aatggtatca
     1861 gaaggctcct gttcatattg agaagagcgg ctttaatcga tttatttggc ttcctgtctt
     1921 ggtttggttc ttatctctgg tcgttcaatt cttcttgcca gatgatcctt cagtaaatca
     1981 acaaatagcg acagacttga ccttgtctca accacttttc tcattctttg ctgtggttat
     2041 ttttgctcct ttgacggaag agattgtttt tagagggatg ttagcacgct atctctttcc
     2101 taagcaggac aatagtaaac gaaccctgat ttttcttctg gtatctagtc ttctatttgc
     2161 cttgattcat tttccaggtg atgtgcaaca attttttgtc tattttagcc ttggttttag
     2221 tttgggtttg gcttacatta gcagaaaagg tctggtctac agtatttctc ttcacgcttt
     2281 gaataattta gtcggctttt tgatgattct catgctataa tagagtcagg aggtcacatg
     2341 aaacgagtaa ttttagtagc agtgattcag gcagtcgttc tatttttcat cattggagcg
     2401 ctagcttatg ccttcaaagg cgatttcttc tataactatc tagcagttgt ctttgctcct
     2461 attgcaggtg tactgcgttt tgggacggct tacataacgg aaattgtctt gcctcgaaag
     2521 gcagccgaaa tcgctgaaaa gcgtaaagca ggcaaaaatt caaaataaaa gagtccggtg
     2581 aagttcatcg gatttttgta ttatactcaa tgaaaatcaa agag
//
LOCUS       DAVBDG010000124         2462 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBDG010000124 DAVBDG010000000
VERSION     DAVBDG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.126"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            143..1501
                     /gene="dcm"
                     /locus_tag="VQ526_002071"
     CDS             143..1501
                     /gene="dcm"
                     /locus_tag="VQ526_002071"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HET2555727.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQT
                     EGEIEYHDIKEVTDHDFRQFRGQVDIICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTFDELGFDVEWQVLNSKDFGVPQNRE
                     RVFIIGHSRKKGTRLLFPFRREGQATNSETLKALGNLNPSKSGVSGKVYYSEGLAPTL
                     VRGKGEGFKVAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFK
                     ETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLE
                     RPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYQVAGVLIDGQFYRLRIRR
                     ITPKECFRLHGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDES
                     IK"
     gene            1485..1934
                     /locus_tag="VQ526_002072"
     CDS             1485..1934
                     /locus_tag="VQ526_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555728.1"
                     /translation="MKALNKESILDCDELETELHDAEIKQLDEQLFLMPNYPCEFEVT
                     FLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDHQNLVFVLYGQSYRAEG
                     KEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNLWKVSHD"
     gene            1927..2307
                     /locus_tag="VQ526_002073"
     CDS             1927..2307
                     /locus_tag="VQ526_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555729.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQKYGIEVTPHSISEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELCIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSS"
     gene            2320..>2462
                     /locus_tag="VQ526_002074"
     CDS             2320..>2462
                     /locus_tag="VQ526_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2555730.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
BASE COUNT          830 a          380 c          530 g          722 t
ORIGIN      
        1 tatattctgt ttggaataac cgtgttagaa aaggaggtgg ttaaatgtcg ccaatcgagt
       61 ggatattagt tatgctcatt ttaatttcaa gtggtgtgac actttggaca ttgatagttg
      121 atcagaaagg ggtaatcaag aaatgagatt tattgattta ttttcaggta tcggtggttt
      181 tcgacttgga atggaaagtg tcggacacga gtgtattgga ttttgtgaga ttgataaatt
      241 tgctagagaa tcttataagt ccatttttca aacggaagga gaaatagaat atcatgacat
      301 taaagaggta acagatcatg actttagaca atttagaggg caagtggaca tcatctgcgg
      361 gggatttcct tgccaagctt tttcacttgc aggaagacga ttgggatttg aagatactag
      421 aggaactttg ttctttgaaa ttgctcgggc ggccaaacaa atccaaccac gttttctttt
      481 tcttgaaaat gttaaaggcc tactcaatca cgataaggga cggacgttca ccacaatcct
      541 taccacattt gatgagttgg ggtttgatgt tgagtggcag gtgcttaaca gtaaggattt
      601 tggcgttccc caaaacagag agagggtgtt tattatcgga cattctagaa agaaaggtac
      661 tagactctta tttcctttca gacgagaagg tcaagcaact aactctgaga ctttgaaagc
      721 actaggaaat ttgaatccat caaaaagtgg cgtgagcggt aaagtgtatt attcagaagg
      781 tcttgcgcca actttagttc gtggaaaagg agaaggattt aaggttgcta ttccttgtat
      841 gacaccagat agattagaca aaagacaaaa tggtagacgc tttaaggaaa accaagagcc
      901 gatgtttact ttaaatactc aggatcgtca tggcattgtc gttgttggag atttaccgac
      961 tagttttaag gagacaggtc gtgtctatgg aagtgagggc ttatctccaa cactgactac
     1021 gatgcaagga ggagataaaa tccccaaaat actaattcca gaaccaatcc aatttctaaa
     1081 agtacgggaa gcaacgaaaa aaggatatgc tcaagcagag attggggatt caatcaattt
     1141 ggaaagacca agttctcagt atcgtcgtgg tagagttggc aaaggtatag ctaatacgtt
     1201 aacaactagt ggtcaaatgg gagtagtagt ggctagctat gaaggagaag ataaacaagt
     1261 ttaccaagta gctggtgttt taatagatgg acaattttac cgtttgagaa tacgacgaat
     1321 cactcctaaa gagtgttttc gattgcatgg ctttcctgat tgggcttttg aagctgctag
     1381 aaaagtctct agtaatagtc agctctataa acaagctggt aatagtgtaa ccgttcctgt
     1441 gattgccgca atcgcaaaga aattaaaaga aatagaggaa aaagatgaaa gcattaaata
     1501 aagaatcaat actagattgt gatgaattag aaacagaatt acatgatgca gaaatcaaac
     1561 agctggatga acaattattt ttgatgccca attatccatg tgagtttgaa gtaacatttt
     1621 tagatgatta ccataaaaaa cacaactacc cactatttta cgagtcctat cttcaaaacg
     1681 ttatggaatt ccttgaaagt caagacataa agaatggggt tgatgccttt gtagatgatc
     1741 atcaaaatct cgtttttgtt ttatatggac aaagctatcg agcagaggga aaagagggaa
     1801 tacttacaac tcaagtaact gtaaaagctt atgatgaaga caagaaaccg attaacttcg
     1861 caaatttatt agattcctta atcgtctcag aatatcaaat ggaaccgaat ctttggaagg
     1921 tctcccatga ttgatctcta tctaagtaaa aatagccaaa gaaatcaact tcttttggat
     1981 ttctttcaaa agtatggcat agaggtcact cctcattcta tttctgaaat gacaaaggac
     2041 aaattaattg aaatgatgag ctattcttca gattgttttg agtttctatc tcccaattta
     2101 ttacgattta agaaccgtga caatctaaga ttaacggatt tcattgaaat gatattaaaa
     2161 aatcctgaac tatgcatcag gcttcctctt gcggtttcaa ataagagagt ttatccaaat
     2221 ctgaatttgg aagaggctag agctctattg ccaagagata cgaaacaatt gatttacatg
     2281 gcacaaacac attatttatc tagttaaagg agaagcaata tggctgaacg attttgggag
     2341 aacttgtcca ttattttggc tgaaagaaat attagctgga ttgagttaac cagaaaaatg
     2401 tttgcgggag agtttcacta tccaagtgaa ttgaatcgtt tgtatcaaaa gatacgccac
     2461 ta
//
LOCUS       DAVBDG010000125         2462 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBDG010000125 DAVBDG010000000
VERSION     DAVBDG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.127"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            143..1501
                     /gene="dcm"
                     /locus_tag="VQ526_002075"
     CDS             143..1501
                     /gene="dcm"
                     /locus_tag="VQ526_002075"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HET2555731.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARKSYKSIFQT
                     EGEIEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIAR
                     ATKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
                     RVFIIGHSRKKGTRLLFPFRREGQATNSEILKTLGNLNPSKSGMSGKVYYSEGLAPTL
                     VRGKGEGFKVAIPCMTPDRLEKRQNGRRFKDNQEPMFTLNTQDRHGIIVVGDLPTSFK
                     ETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLE
                     RPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGVLIDGQFYRLRIRR
                     ITPKECFRLQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIAKKLKEVEEKDES
                     FK"
     gene            1485..1934
                     /locus_tag="VQ526_002076"
     CDS             1485..1934
                     /locus_tag="VQ526_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555732.1"
                     /translation="MKALNKESILDCDELETELHDAEIRQLDEQIFLMPNYPCEFEVT
                     FLDDYHKKHNYPLFYESYLQNIMEFLESQDIKNGADAFVDDHQNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKQPINFANLLDSLIVSEYQMEPNLWEVSHD"
     gene            1927..2307
                     /locus_tag="VQ526_002077"
     CDS             1927..2307
                     /locus_tag="VQ526_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555733.1"
                     /translation="MIDLYLSKNNHRNQLLLEFFQNYGIEVSCHSISEMTRDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELTIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            2320..>2462
                     /locus_tag="VQ526_002078"
     CDS             2320..>2462
                     /locus_tag="VQ526_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2555734.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
BASE COUNT          823 a          379 c          538 g          722 t
ORIGIN      
        1 tgtattctgt ttggaataac cgtgttagaa aaggaggtgg ttaaatgtcg ccaatcgagt
       61 ggatattagt tatgctcatt gtcatttcaa gtggagtgac tctttggaca ttgatagtcg
      121 atcaggaagg ggtgatcaag aaatgaggtt tattgattta ttttcaggta tcggtggctt
      181 tcgacttgga atggaaagtg tcggacacga gtgtattgga ttttgtgaga ttgataaatt
      241 tgctaggaaa tcttataagt ccatttttca aacggaagga gaaatagaat ttcatgacat
      301 acgagatgtt tcagatgacg aatttaaaaa acttagaggg aaagtcgatg tcatctgtgg
      361 gggattccct tgtcaagcat tttcaatcgc aggaagacga ttgggatttg aagatactag
      421 aggaactttg ttctttgaaa ttgctcgagc gaccaaacaa atccaaccac gttttctttt
      481 tcttgaaaat gttaaaggcc tactcaatca cgataaggga cggacgttca ccacaatcct
      541 taccacactt gatgagttgg ggtttgatgt tgagtggcag gtgcttaaca gtaaggattt
      601 tggcgttccc caaaacaggg agagggtgtt tattatcgga cattctagaa agaaaggtac
      661 cagactctta tttcctttca gacgagaagg tcaagcaact aactctgaga ttttaaaaac
      721 attagggaat ttgaatccat caaaaagtgg aatgagcggt aaagtctatt attcagaagg
      781 tcttgcgcca accttagttc gtggtaaagg agaaggattt aaagttgcga ttccttgtat
      841 gacaccagat agattagaaa aaagacaaaa tggtagacgt ttcaaggata atcaagagcc
      901 aatgtttact ttaaatactc aggatcgcca tggtattatc gttgttggag atttaccgac
      961 tagttttaag gagacaggtc gtgtctatgg aagtgagggc ttatctccaa cactgactac
     1021 gatgcaaggt ggagataaaa tccccaaaat actgattcca gaaccaatcc aatttctaaa
     1081 agtacgggaa gcaacgaaaa aaggatatgc tcaagcagag attggggatt caatcaattt
     1141 agaaagacca agttctcagt atcgtcgtgg tagagttgga aaaggtatag cgaatacgtt
     1201 aacaactagt gggcaaatgg gagtagtagt ggctagctat gaaggggaag ataagcaagt
     1261 ttatcatgta gccggtgtct taattgatgg gcaattttac cgtttgagga tacgaagaat
     1321 cacccctaaa gagtgtttta gactgcaggg atttcctgat tgggcttttg aagctgctag
     1381 aaaagtctct agtaatagtc agctctataa acaagctggt aatagtgtaa ccgttcctgt
     1441 gattgccgca atcgcaaaga aattaaaaga agtagaggaa aaagatgaaa gctttaaata
     1501 aagagtcaat actagattgt gatgaattag aaacagaatt acatgatgca gaaatcagac
     1561 agctggatga acaaatattt ttgatgccca attatccatg tgagtttgag gtgacatttt
     1621 tagatgatta ccataaaaaa cacaactacc ccctatttta cgaatcctat cttcaaaaca
     1681 ttatggaatt ccttgaaagt caggatataa agaacggagc tgatgccttt gtagatgatc
     1741 atcaaaatct tgtttttgtt ttatatggac aaggctatcg agccgaggga aaagagggaa
     1801 tacttacaac ccaagtaact gtaaaagctt atgatgaaga caagcaaccg attaacttcg
     1861 caaatttatt agattcctta atcgtatcag aatatcaaat ggaaccgaat ctttgggagg
     1921 tttcccatga ttgacctcta tctaagtaaa aataaccata gaaatcaact tcttttagaa
     1981 ttcttccaaa actatggcat tgaggtatcc tgtcattcga tttctgaaat gacaagggat
     2041 aaattaattg agatgatgag ctattcttca gattgttttg agtttttatc tcctaattta
     2101 ttacgattta agaaccgtga caatttaaga ctaacggatt tcattgaaat gatattaaaa
     2161 aatcctgaac taactattag acttcctctt gcggtttcaa ataagagagt ttatccaaat
     2221 ctgaatttgg aagaggctag agctttattg ccaagagata cgaaacaatt gatttacatg
     2281 gcacaaacac attatttatc taactaaagg agaatcaata tggctgaacg attttgggag
     2341 aacttgtcca ttattttggc tgaaagaaat atcagctgga ttgagttaac cagaaaaatg
     2401 tttgcgggag agtttcacta tccaagtgaa ttgaatcgtt tgtatcaaaa gattcgccac
     2461 ta
//
LOCUS       DAVBDG010000126         2364 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBDG010000126 DAVBDG010000000
VERSION     DAVBDG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.128"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..1523
                     /gene="phtA"
                     /locus_tag="VQ526_002079"
     CDS             <1..1523
                     /gene="phtA"
                     /locus_tag="VQ526_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2555735.1"
                     /translation="FDPAQITSRTARGVAVPHGDHYHFIPYSHMSELEERIARIIPLR
                     YRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSNSSLVSQLVRKVGEGYVFEEK
                     GISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKENVAPRDQEFYDKAYNLLTEA
                     HKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDLLAFLAPITHPERLGKPNSQI
                     EYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYVTPHMGHSHWIGKDSLSDKEK
                     VAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVKGEKRIPLVRLPYMVEHTVEV
                     KNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLFATIKYYVEHPDERPHSNDGW
                     GNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVPQVETEKVEAQLKEAEVLLAK
                     VTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKLLALLKGSNPSSVSKEKIN"
     gene            1681..>2364
                     /locus_tag="VQ526_002080"
     CDS             1681..>2364
                     /locus_tag="VQ526_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2555736.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAA"
BASE COUNT          831 a          431 c          476 g          626 t
ORIGIN      
        1 ttttcgaccc agcgcaaatc acaagtcgaa cagctagagg tgttgcagtg ccacacggag
       61 atcattacca cttcatccct tactctcaca tgtctgaatt ggaagaacga atcgctcgta
      121 ttattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaaggcca gaacaaccaa
      181 gtccacaacc gactccggaa cctagtccag gcccgcaacc tgcaccaaat cttaaaatag
      241 actcaaattc ttctttggtt agtcagctgg tacgaaaagt tggggaagga tatgtattcg
      301 aagaaaaggg catctctcgt tatgtctttg cgaaagattt accatctgaa actgttaaaa
      361 atcttgaaag taagttatca aaacaagaga gtgtttcaca cactttaact gctaaaaaag
      421 aaaatgttgc tcctcgtgac caagaatttt atgataaagc atataatctg ttaactgagg
      481 ctcataaagc cttgtttgaa aataagggtc gtaattctga tttccaagcc ttagacaaat
      541 tattagaacg cttgaatgat gaatcgacta ataaagaaaa attggtagat gatttattgg
      601 cattcctagc accaattacc catccagagc gacttggcaa gccaaattct caaattgagt
      661 atactgaaga cgaagttcgt attgctcaat tagctgataa gtatacaacg tcagatggtt
      721 acatttttga tgaacatgat ataatcagtg atgaaggaga tgcatatgta acgcctcata
      781 tgggccatag tcactggatt ggaaaagata gcctttctga taaggaaaaa gttgcagctc
      841 aagcctatac taaagaaaaa ggtatcctac ctccatctcc agacgcagat gttaaagcaa
      901 atccaactgg agatagtgca gcagctattt acaatcgtgt gaaaggggaa aaacgaattc
      961 cactcgttcg acttccatat atggttgagc atacagttga ggttaaaaac ggtaatttga
     1021 ttattcctca taaggatcat taccataata ttaaatttgc ttggtttgat gatcacacat
     1081 acaaagctcc aaatggctat accttggaag atttgtttgc gacgattaag tactacgtag
     1141 aacaccctga cgaacgtcca cattctaatg atggatgggg caatgccagt gagcatgtgt
     1201 taggcaagaa agaccacagt gaagatccaa ataagaactt caaagcggat gaagagccag
     1261 tagaggaaac acctgctgag ccagaagtcc ctcaagtaga gactgaaaaa gtagaagccc
     1321 aactcaaaga agcagaagtt ttgcttgcga aagtaacgga ttctagtctg aaagccaatg
     1381 caacagaaac tctagctggt ttacgaaata atttgactct tcaaattatg gataacaata
     1441 gtatcatggc agaagcagaa aaattacttg cgttgttaaa aggaagtaat ccttcatctg
     1501 taagtaagga aaaaataaac taatgaaaaa tgaaagtctc gataaagagg ctttcatttt
     1561 tattatgtat atatgtaaaa ttcttgacaa gcaatattaa aaagagtaaa ctattaacta
     1621 gttaattaac cggtttatta ctttatagtg aatcaaatat acttaagaaa agaggaaaga
     1681 atgaaaatca ataaaaaata tctagctggg tcagtagcta cacttgtttt aagtgtctgt
     1741 gcttatgaac taggtttgca tcaagctcaa actgtaaaag aaaataatcg tgtttcctat
     1801 atagatggaa aacaagcgac gcaaaaaacg gagaatttga ctcctgatga ggttagcaag
     1861 cgtgaaggaa tcaatgccga acaaatcgtc atcaaaataa cagaccaagg ctatgtcact
     1921 tcacatgggg accactatca ttattacaat ggtaaggttc cttatgacgc tatcatcagt
     1981 gaagaattgc tgatgaagga tccaaactac cagctaaagg acgaggatat tatcagtgaa
     2041 atcaagggtg gttatgtgat taaggtagat ggaaaatact atgtttacct taaggatgca
     2101 gctcatgcgg ataatgtccg tacaaaagaa gaaatcaatc gacaaaaaca agagcatagt
     2161 caacatcgtg aaggtgggac tccaagaaac gatggtgctg ttgccttggc acgttcacag
     2221 ggacgctaca ccacagatga tggttatatc tttaatgctt ctgatatcat tgaagatact
     2281 ggtgatgctt atatcgttcc tcacggcgac cattaccatt acattcctaa gaatgagtta
     2341 tcagctagcg agttagctgc tgca
//
LOCUS       DAVBDG010000127         2120 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBDG010000127 DAVBDG010000000
VERSION     DAVBDG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.129"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            69..608
                     /locus_tag="VQ526_002081"
     CDS             69..608
                     /locus_tag="VQ526_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2555737.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTALLRASQF"
     gene            675..1085
                     /locus_tag="VQ526_002082"
     CDS             675..1085
                     /locus_tag="VQ526_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2555738.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1201..>2120
                     /gene="pavB"
                     /locus_tag="VQ526_002083"
     CDS             1201..>2120
                     /gene="pavB"
                     /locus_tag="VQ526_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2555739.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKSVDININGLVAKETVEKAVK"
BASE COUNT          647 a          406 c          471 g          596 t
ORIGIN      
        1 acgttgacgc ggtttgaaga gattttcgaa gagtataaga aaaaatcagt cccctaaagg
       61 agcagattat gaagttattg tctatcgcca tttctagcta taatgcagca gcctatcttc
      121 attactgtgt ggagtcgcta gtgattggtg gtgagcaagt tgggattttg attatcaatg
      181 acgggtctca ggatcagact caggaaatcg ctgagtgttt agctagcaag tatcctaata
      241 tcgttagagc catctatcag gaaaataaat gccatggcgg tgcggtcaat cgtggcttgg
      301 cagaggcttc tgggcgctat tttaaagtag ttgacagtga tgactgggtg gatcctcgtg
      361 cctacttgaa aattcttgaa accttgcagg aacttgagag caagggtcaa gaggtggatg
      421 tctttgtgac caattttgtc tatgaaaagg aagggcaatc tcgtaagaag agtatgagtt
      481 acgattcagt cttgcctgtt cggcagattt ttggctggga ccaggtcgga aatttctcca
      541 aaggccagta taccatgatg cactcgctga tttatcggac agctttgttg cgtgctagcc
      601 agttctaact gcctgaacat actttttatg tcgataatct ctttgtcttt acgccccttc
      661 agcaggtcaa gaccatgtac tatctgcctg tcgatttcta tcgttatttg attgggcgtg
      721 aggaccagtc tgtcaatgag caagtgatga ttaagtgcat tgaccagcaa ctcaaggtca
      781 atcgactctt ggtagaccaa cttgatttgt cccaagtgag tcatcccaaa atgcgagaat
      841 atctgctgaa tcatattgaa atcacgacgg tgatttccag taccctgctc aaccgatctg
      901 gaacagcgga gcatctggca aaaaaacgcc aattgtggac ctatattcag cagaaaaatc
      961 cagaagtctt tcaggctatt cgtaagacca tgttgagccg tttgaccaaa cattctgtct
     1021 tgccagatcg caaactgtcc aatgtcgtct atcaaatcac caaatctgtt tatggattta
     1081 attaatataa gtgttttata agagggattt aagaaaaatt ttaacttttt cttagtcctt
     1141 tttaatttca ggagattata ctagagtcat caaataaaga aagactctaa ggagaatcct
     1201 atgaaattca atccaaatca aagatatact cgttggtcta ttcgccgtct cagtgtcggt
     1261 gttgcctcag ttgttgtggc tagtggcttc tttgtcctag ttggtcagcc aagttctgta
     1321 cgtgccgatg tggtcaatcc gaccccaggt caagtcttac ctgaagagac atcgggaacg
     1381 aaagagggtg acttatcaga aaaaccagga gacaccgttc tcactcaagc gaaacctgag
     1441 ggcgttactg gaaatacgaa ttcacttccg acacctacag aaagaactga agtgagcgag
     1501 gaaacaaact cttctagtct ggatacactt tttgaaaaag atgaagaagc tcaaaaaaat
     1561 ccagagctaa cagatgtctt aaaagaaact gtagatacag ctgatgtgga tgggacacaa
     1621 gcaagtccag cagaaactac tcctgaacaa gtaaaaggtg gagtgaaaga aaatacaaaa
     1681 gacagcatcg atgttcctgc tgcttatctt gaaaaagctg aagggaaagg tcctttcact
     1741 gccggtgtaa accaagtaat tccttatgaa ctattcgctg gtgatggtat gttaactcgt
     1801 ctattactaa aagcttcgga taatgctcct tggtctgaca atggtactgc taaaaatcct
     1861 gctttacctc ctcttgaagg attaacaaaa gggaaatact tctatgaagt agacttaaat
     1921 ggcaatactg ttggtaaaca aggtcaagct ttaattgatc aacttcgcgc taatggtact
     1981 caaacttata aagctactgt taaagtttac ggaaataaag acggtaaagc tgacttgact
     2041 aatctagttg ctactaaaag tgtagacatc aacatcaatg gattagttgc taaagaaact
     2101 gtcgaaaaag ctgttaaaga
//
LOCUS       DAVBDG010000128         2076 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBDG010000128 DAVBDG010000000
VERSION     DAVBDG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.130"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..1445)
                     /locus_tag="VQ526_002084"
     CDS             complement(<1..1445)
                     /locus_tag="VQ526_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2555740.1"
                     /translation="MKEVDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVI"
BASE COUNT          605 a          468 c          382 g          621 t
ORIGIN      
        1 ttaataacgt tgatatgatt gacctctgga tcttccgtcc cttggaggga gcaaccacgt
       61 tccttggcga attggatata gtctacaatt tctcgtgtaa tgcgttcacc aggagccaag
      121 ataggaatcc ctggaggata gcacatgaca aattctccac agacctgtcc aacagactca
      181 tctaaggtca aactttttct ctctgaatag aaggcttctt gtggagacag caccaactcg
      241 ggctgaatat attctccagc tattaagtcc ttcccatctc gtgaatagag tctcttaata
      301 tcagccagag caccaaccaa gcgctcgatg tcttggatgc ggtcgccgat ggaaatatag
      361 gccaagatat tgccgatatc accaaactcg atctgaatgt cgtattcgtc tcgcaagagg
      421 tcataaacct cgatacctgt taagcctatt ccctgagtgt aaactgacaa cttggttaca
      481 tcaaaatcac agaccgacac accatctatt aactcttttg agtaagcata gtaaccgcca
      541 atggcattga tttcatgacg agcgtactcg gatagctcaa tgacttcctc aaacgactct
      601 ttaccacgaa gggccaagtt gcgacgtgaa atatccaaac tagccatcaa taagtaagag
      661 gcagatgtag actgggtcag gtttataatc tgacgaacgt actcgggatt catctgctcc
      721 ccgattaaaa gaagcgaact ttgtgtcaaa ctcccaccag acttatgcat ggaaactgct
      781 gccatatcag ctcctgcatc catagcagaa attggaagtt taccagtgaa atgcaaatgc
      841 gctccatggg cttcatctac taaaaccatc atgccagctt catgagccat ttctgtcaac
      901 ccctttaggt ctgaacagat gccgtagtaa gtaggattgt tgattaggat agccttagca
      961 tctggatggt cctttatggc ctgtgctact cggtcatttt caagacctaa agcgatacca
     1021 atcttaggat ctacactcat ctcgatatag atgggaatgg caccacatag aaccaacgca
     1081 ttgatagcag atttatggac atttcgtggc agaataatct tatctcctgc cttgcaggtt
     1141 gccagaatca tagtctgcac cgatgaagtt gttccaccaa tcattagaaa ggcatggcta
     1201 gctccaaaag catctgcagc cagctcctct gcatcacgaa taatcgaaat aggatggcct
     1261 aaattatcca agggtttcat cgaattgaca tcaatgccta cacatttttc tcctaagagt
     1321 tcgacaagtt ctggatttcc ccgtccacgc ttgtgacctg gaacatcaaa gggaacaatc
     1381 cttttcttgc gtaacttcac caaggcctca taaattgggg cttggttttg atctacctct
     1441 ttcaaaacat actcctttca atatttttag gaccttatga aaactaagga actaaaggtt
     1501 gactcatgaa aaaagagcag gttttcacct gctctgcaat cttctgacgt gtttattttg
     1561 gtttctgttg agtatgtctg ttcccgatgt ttcctaactc tctagtagca aatattacgt
     1621 actttattga tcgtttcaca agatgacgtg tgctttcacc acactaaaaa tttatgaatt
     1681 aggacaagtg tcctaacatc aacttataaa agtaggcttt taaccctatc aataatgtgg
     1741 cttttcaacc acgcttcggc attcaaccct gcctgtccgt aggcattttt tactcgggtt
     1801 tatatttgct agaaaacatc atctcatttt ctaagcttta ttactatatc atgtttttaa
     1861 gaacttgtaa agtatttttt gataaaaaac aaaaatataa tagattgaaa ctagaatagt
     1921 acacttctgc ttctaaaaca ttattagaaa tagattttac tgtcctgatc gatttgttct
     1981 gttcttgttt cattttacta tatgttcgta aatgaaagtt ctataatgaa gttagccacc
     2041 cagccctcct aaaaattaga tggttcaact agctga
//
LOCUS       DAVBDG010000129         1899 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBDG010000129 DAVBDG010000000
VERSION     DAVBDG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.131"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(421..1617)
                     /gene="tuf"
                     /locus_tag="VQ526_002085"
     CDS             complement(421..1617)
                     /gene="tuf"
                     /locus_tag="VQ526_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2555741.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          537 a          365 c          377 g          620 t
ORIGIN      
        1 ttttgatggt ttggatttct tctttagttg atttcatatc actattgtat aatgcttttt
       61 gattttagtc tagtattata ctcttcgaaa atctcttcaa accgcgtcag cttcgccttg
      121 tcgtatagat gttactgact tcgtcagttc tatctacaac ctaaaaacag tgttttgagc
      181 agtctgtagc taactttcta tagtaaaatg aaataagaac aagacaaatc gatcaggaca
      241 gtcaaatcga tttctaacaa tgttttagaa gtaaatatgt actattctag tttcaatata
      301 ttatagtttg ctctttgatt ttcattgagt attagttcaa tatctaaaaa aagaaccttg
      361 ccaagcaaga ttcttttagt gtctgactta aataattgtt cttctgggaa ctaaatcgaa
      421 ttaagcttcg atttctgtaa ccatacctga accaacagta cgtccaccct cacggataga
      481 gaatgtagta ccttgttcta cggcgattgg gtgaatcaac tcaacgtcga ttgtcacgtt
      541 atcaccaggc attaccattt cagtacctgc tggaagttcg attgaacctg taacgtcagt
      601 agtacggaag tagaattgtg gacggtagtt gttgaagaat ggagtgtgac gtccaccttc
      661 ttctttagta aggatgtaga cttcaccttt gaatttagtg tgtgggttga ttgaacctgg
      721 tttagcgata acttgtccac gttcgatttc atcacgttga acaccacgaa gaaggacacc
      781 tacgttatct ccagcaagac cttcgtcaag ttgtttacgg aacatttcaa caccagtaac
      841 aactgctttt tgagtttctt ctttgatacc aacgatttcg atttcgtcgt tgactttaac
      901 gataccacgg tcgatacgtc ctgaagcaac tgtaccacgt ccagtgattg agaatacgtc
      961 ctcgactgga agaagcaatg gtttgtcagt gtcacgttct ggttctggga tatactcatc
     1021 aactgtgttc atcaattcca taacgatgtc ttcgtattta gagtcacctt caagagcttt
     1081 aagtgctgaa ccttggataa ctggaagatc gtcacctggg aagtcgtatt ctgacaatag
     1141 gtcacggatt tccatttcaa ccaattcaag caattcttcg tcgtcaacca agtcaacttt
     1201 gttcatgaag acgataaggt gtttaacacc aacctgacgt gaaagaagga tgtgctcacg
     1261 agtttgtggc attggtccgt cagttgaagc tactacaagg atagctccgt ccatttgagc
     1321 agcaccagtg atcatgtttt taacgtagtc cgcgtgtcct ggagcgtcga tgtgagcgta
     1381 gtgacgtttt tcagtttcgt actcaacgtg cgcagtgttg atagtgatac cgcgttcgcg
     1441 ttcttctgga gcagcatcga tagacgcata gtctttaggt tggttaactg atgaaggcaa
     1501 gcgacgtgcc aaaacagttg tgatagctgc agttagggta gttttaccgt ggtcaacgtg
     1561 tccgatagta ccaatgttaa cgtgtggttt actacgatcg tatttttctt ttgccatttg
     1621 agtaaaagcc tccaataaaa tatattttat agatagacag taggcaatac agtctaactt
     1681 tccttactat tttatcaaat ttcagctaaa ttgcaagtgt tttacaacgt ttttgtgaat
     1741 tattcttggt aaaaatttaa ctaactaata gaaagtacta aatagactta tccttcaagg
     1801 agttttcagc attatccatc cactcacttg ctgaaaatgt aaacattctc tggtttatat
     1861 agtaaaatga aacaagaata gggcaaatcg atcaggaca
//
LOCUS       DAVBDG010000130         1455 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBDG010000130 DAVBDG010000000
VERSION     DAVBDG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.132"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..559
                     /locus_tag="VQ526_002086"
     CDS             <1..559
                     /locus_tag="VQ526_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2555742.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="VQ526_002087"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="VQ526_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HET2555743.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          504 a          208 c          266 g          477 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagggat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgc
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcggggg ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg tcgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtga
//
LOCUS       DAVBDG010000131         1423 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBDG010000131 DAVBDG010000000
VERSION     DAVBDG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.133"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(339..1319)
                     /locus_tag="VQ526_002088"
     CDS             complement(339..1319)
                     /locus_tag="VQ526_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2555744.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVDDQVYNWLCGSEIPFKYLK"
BASE COUNT          429 a          317 c          262 g          415 t
ORIGIN      
        1 tttttgaaaa gtattaacac ctacagacaa gcctgacgat agcaagaact accctactcg
       61 ataggtatcg gcttttgctt tctgaaaaaa attattttaa gcaatttgac aaatctagca
      121 acaaaaaatt ttataaatac aatagattga aactagaata gtacaccttt gcttctaaaa
      181 cattgttaga aatcgatttg actgtcctga tcgatttgtc ctgtttttat ttcattttac
      241 tatatttcca tgataaaacg catagtatca agttttctta atcccctgat actatgcgta
      301 tttgtaattt ttaagatttt gtgcttagag tcgactcctt attttagata tttaaaagga
      361 atctcactcc cacagagcca gttgtagact tggtcatcga caaatacatt catggcttcg
      421 tgagcatact caggcatgat gcgataggtt ttatcgcagg tcagacgatt ataaatcgca
      481 aactgggtaa tgggatagca aacatcgtcg tccaagcccg taatcatctt aacctcacct
      541 tggatacgat gggcaagatt tttgacatcg atataggcaa gggtcgccat gatttcctcc
      601 tctgtttcat ggaaggggtc gtgaaacttg aaataacgga aaagttcgtc gtaagcctcg
      661 ctagtattac caatctcaat cacccgtctg aagtctgaca agaagggata aatggcaact
      721 gttttctgaa ttcgaggatt gagcgctgct gcaactagag ctagagcccc tccttgtgag
      781 gcaccatagc tagaaagacg cttctcatca acctgagaca gactagcaac aatttcgacc
      841 aactggtaaa tatccagata aacatcctta taaaagaggt ggtcccgacc ttccatagca
      901 ccacggataa tatgcccctt cacggtattt cctaaaggag aacgcaagcc gtcttgtgag
      961 taacctgact ggccccgcac atccatggaa acaacaccgt aaccagctac ggtgaagccc
     1021 agcatgtcgg cccagtccca gccacgtccc atataaccat gaaaatggaa gattagtgga
     1081 accttctcct cactctttgg aagaacaatg cgtgcataga cctttccttc cttgcttcct
     1141 tcaaatgtta actcatagca cttgacttga ggaatgtgga aatctctttc ctccaagtgg
     1201 taggatggaa gcgtggaaac atttttcact tccccatccc agaaagcatc aaagtcttcc
     1261 ggaacctcat cccttcctct ataggtctta atttcttcta gcaaagctgg atttttcata
     1321 gcatgctcct tttattttgt aatcgttacc acaactgtag catatctatg taatcaatgc
     1381 aagaaagaag gataaataga aagtgaattt agattcttta ctt
//
LOCUS       DAVBDG010000132         1402 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBDG010000132 DAVBDG010000000
VERSION     DAVBDG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.134"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            283..1293
                     /gene="ccpA"
                     /locus_tag="VQ526_002089"
     CDS             283..1293
                     /gene="ccpA"
                     /locus_tag="VQ526_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2555745.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
BASE COUNT          438 a          229 c          317 g          418 t
ORIGIN      
        1 tcctgttctt atttcatttt actataactt taggagggct tagtttcctc ctttttgttt
       61 agtctaaggt ggtagcaagg agctaaacct aggttttcta tgaaattttt tcaatcaatc
      121 ttcaaaaaac acttgaaagt gtttacaatt agtgatagaa tggaataagt agaaatatga
      181 aaacgaaatt ttcacatctt cacttttgta agtagatata gagcttcaat cagcacctct
      241 acttgtaaaa gaatatgtga gatagaaagg acaggaaaag gaatgaatgc agatgataca
      301 gtaaccattt atgatgtcgc tcgtgaagca ggtgtttcca tggcgacggt cagccgtgtg
      361 gtcaatggca ataaaaatgt aaaagagaat acccgtaaaa aagtgctaga ggtaattgat
      421 cgtttggatt atcgtccaaa tgcagttgcg cgtggtcttg caagtaaaaa gacaaccact
      481 gtcggtgtcg tgattccaaa tattaccaat ggttattttt cgagtttggc taaggggatt
      541 gatgatattg cagaaatgta caagtacaat attgtcctag ctaatagcga tgaagataac
      601 gagaaagaag tttctgttgt caataccctc ttttcaaagc aggtagatgg cattatctat
      661 atgggttatc acttgacaga taaaattcgc tcagaatttt cgcgctcacg tactccgatt
      721 gttctcgcag gaactgtcga tgttgagcac cagttgccaa gtgtcaatat tgactataag
      781 caagcaacaa ttgatgcagt gagttacctt gctaaagaaa atgagcgtat tgctttcgtt
      841 agcggtccgc tagtggatga catcaatggt aaggttcgtt tagttggcta caaggaaacc
      901 ttgaaaaaag caggaatcac ttatagtgag ggtttggtat ttgaatctaa atatagctat
      961 gatgatggtt acgccttagc agagcgtttg atttcatcaa atgcaactgc agcagttgtg
     1021 acaggtgatg agttggcagc aggagtcttg aacggtttgg ctgataaagg tgtttctgtg
     1081 ccagaagatt ttgaaattat tactagtgat gattcacaaa tctcacgctt tacccgtcca
     1141 aacttgacaa cgattgccca acctctttat gaccttggtg ctattagtat gcgtatgttg
     1201 accaagatta tgcataagga agagttggaa gaacgtgaag ttctcttacc tcatggtttg
     1261 acagaacgta gctcaacacg aaaacgtaaa tagaaaaaat cagggaatcg agaagatttc
     1321 ctgattttgt gctcttaaac aagagatgtt gacttaatga ataccataga gcggactagg
     1381 cggctggtta aaggctgcgc aa
//
LOCUS       DAVBDG010000133         1132 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBDG010000133 DAVBDG010000000
VERSION     DAVBDG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.135"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQ526_002090"
     CDS             84..491
                     /locus_tag="VQ526_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2555746.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKID"
     gene            656..955
                     /locus_tag="VQ526_002091"
     CDS             656..955
                     /locus_tag="VQ526_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2555747.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
BASE COUNT          395 a          226 c          195 g          316 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat ttaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaggtgc cgaaatgaac tgcaccccaa aagttagaca attaatttat ccgaaggatt
     1081 tagttctgta ttgcacaggg ctaagtcctt ttagttttac cttaattctc tt
//
LOCUS       DAVBDG010000134         1049 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBDG010000134 DAVBDG010000000
VERSION     DAVBDG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.136"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            2..547
                     /locus_tag="VQ526_002092"
     CDS             2..547
                     /locus_tag="VQ526_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2555748.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKINQLWK"
     gene            complement(495..>1049)
                     /locus_tag="VQ526_002093"
     CDS             complement(495..>1049)
                     /locus_tag="VQ526_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2555749.1"
                     /translation="KDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTE
                     IPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPA
                     THNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPIS
                     NHEEMKRIISKVDLFFQKVSQLFE"
BASE COUNT          354 a          150 c          166 g          379 t
ORIGIN      
        1 tatgaattgg gttgttataa aaataaaaga tattttttca atgaatacag gtctttctta
       61 caagaagggc gatttaagca ttaataataa aggtgttaga attatacgtg gtggtaatat
      121 taagccttta gaattttctc tgttggataa tgattactac attgatacac aattcatctc
      181 ctctgaacaa gtttatttaa aacataatca gctaataaca cctgtatcaa cctctttaga
      241 acatattgga aagtttgcaa gaatcgataa agactatgat ggtgttgtgg ctggtggatt
      301 tattttccaa ttaacaccat tcgaaagttc agagattatt tcaaaatttc tattatttaa
      361 cttgtcttct ccgttatttt ataaacaatt gaaagcaata actaaactat caggtcaagc
      421 tttatataat attcctaaaa ctacactgag cgagctatta attccgttag ctccttttga
      481 ggaacaggaa cttattactc aaaaagttga gaaacttttt gaaaaaataa atcaactttg
      541 gaaatgattc ttttcatctc ttcatgatta gaaataggga ttaataattc ggagatactg
      601 gtactattta atgttttccc tttgatagca tcttttgaat cacctaaagt agagataagt
      661 ggcaaaaata tcattaagta atctctgata atattttctt tattagcata ggggaatatc
      721 gatataatgg cttcattatg agtggcagga atatccaata tggcaacttt tccaatagat
      781 aatttaaaac tcattaataa agttccttta ggtgaaatgt ctattttctt tgattttaat
      841 gctaatttag aaatagattc tctcgtatta gttacataac cagatatagg catatctgat
      901 atagataccc aaggtatttc agttccccaa aaagtagctt cactgcgtgg aggagttttt
      961 cctattctga agttaactag gctagcaaat ttaatatatc tccatgcttc tgggatttca
     1021 tatataggat aagaggttgt ttcgtcttt
//
LOCUS       DAVBDG010000135         1039 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBDG010000135 DAVBDG010000000
VERSION     DAVBDG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.137"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(748..1008)
                     /locus_tag="VQ526_002094"
     CDS             complement(748..1008)
                     /locus_tag="VQ526_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555750.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELELVKSTILQNDDISLDQNEIFEILKNFFDG"
BASE COUNT          348 a          197 c          164 g          330 t
ORIGIN      
        1 tatatatatg cacagacata tgaaatcttt ttgatacagc cttcttttta catagagaga
       61 cagcagttat gatatattta tccattcaca acatcttctg taccaatact acaataattg
      121 gtacattttg attacacaat cgtttcaaaa aatatgatgg aagactgagt attttatagt
      181 aattcccttc ctatcaacta gatcaaccag ttgagtgttg aatgttgaaa caccctagcc
      241 tcagcaaagg actatgatca gcatttccta gccaatagga cgctccaaat cacctattct
      301 aagtccatca ctagcttggt cagtaagata cagactgcaa ggcaagactg acgtggttga
      361 tgagattttg gaagagtatt acaatcatag attcaccagt agacttgacc gcagatttaa
      421 ctatagtaga ttgaaactag aatagtacac ttctgcttct aaaacattgt tagaaatcga
      481 tttgactgtc ctgatcaatt tgtcctgttc tcgtttcatt ttactatact aaaaaaccac
      541 ctgaaacaaa tcaagtgact agaggataaa aacaaagact gggcaaaaag ttttgaaaag
      601 caaaaaacgc ataatatcag gtcttgaaaa accttgatac tatacgtttg attgtgggaa
      661 gattgacttc attttctcct gaaattgaag ttttttctag tttccaggta tagacgaatt
      721 caatatagcc atctcgctac aattatttta accatcgaaa aaatttttca gaatttcaaa
      781 aatttcattt tgatctaatg aaatgtcatc attttgaaga atagttgact tcacaagctc
      841 taattctatc acaaagcggt gcatgtctgc ttttaccgca ctacttgatg ttatagccga
      901 taacttttca tctcctagca aggaagctgt caaacgggta aggtcattca aatgcttttt
      961 aatggactta cttaaacctt gagcacctgt agcagagcgt tccctcatat ccagccaagc
     1021 tttagcctta aaaataatt
//
LOCUS       DAVBDG010000136          963 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBDG010000136 DAVBDG010000000
VERSION     DAVBDG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.138"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..464
                     /locus_tag="VQ526_002095"
     CDS             <1..464
                     /locus_tag="VQ526_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2555751.1"
                     /translation="YAMFDKEQKRGYKQSARNLANWHYNDKEDSYTHPDGWYYRFHHT
                     KYQKTQTDFQQKIKVYYADEPESAPQKGLYMNERYQNLKAKECQALLSPQGRQIFAQR
                     KIDVEPVFGQIKACLGYKRCNLRGKRQVRIDMRLVLMANNLLKYNKRTTQN"
     gene            complement(614..808)
                     /locus_tag="VQ526_002096"
                     /pseudo
     CDS             complement(614..808)
                     /locus_tag="VQ526_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(835..>963)
                     /locus_tag="VQ526_002097"
     CDS             complement(835..>963)
                     /locus_tag="VQ526_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2555752.1"
                     /translation="LKINEDLNIGSVRSPLTPVTEEDRPVVEEAAALIRETKERFL"
BASE COUNT          319 a          182 c          188 g          274 t
ORIGIN      
        1 aatatgccat gtttgataag gaacaaaaga gaggctataa acagtcagct agaaacttag
       61 cgaattggca ctataatgac aaggaggata gctatacaca tcctgatggc tggtattatc
      121 gttttcacca taccaaatat cagaaaacac agacagactt tcaacaaaaa atcaaggttt
      181 actacgccga cgaacctgaa tcagcccctc aaaagggact gtatatgaac gaacgctatc
      241 aaaacttgaa agctaaagaa tgtcaggcgc ttttatctcc ccaaggtaga cagattttcg
      301 ctcaacgcaa gattgatgtg gaacctgtct ttgggcagat aaaggcttgt ttgggttaca
      361 agagatgtaa tctgagaggg aagcgtcaag tgagaattga catgagattg gtacttatgg
      421 ccaataacct cctaaaatac aataagagaa cgactcaaaa ttaaaaagct agagttccac
      481 aattggaaat ctttagcttt tttgtgattg agaactattt tatcctagac tcttttgatt
      541 tttaaagaga acttaaatca ttctctaatt aaagtaggaa gaatacacta atttagtaaa
      601 gtaaatatta aatttatttt attttaaaat gatagtaggc tccaagcata tcagcagtgt
      661 tttgatgatg agcaaattct aatcttgtgt tctcagccaa acttggaacc aaggcttctt
      721 tcaaggctgc acgaatcttt ggtttgagga tagcttcttg tcccatgatg ccacccccga
      781 gaatgaccac ctctggattg gcaacgtagt gcgtcataaa tacctcctta tggattagag
      841 gaagcgctcc ttggtttcac gaatcaaggc agcagcttct tctacaactg gacgatcttc
      901 ttcagtcact ggtgtcaatg gtgaacgaac agatccaata ttcaagtctt cattgatctt
      961 caa
//
LOCUS       DAVBDG010000137          941 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVBDG010000137 DAVBDG010000000
VERSION     DAVBDG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.139"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(370..768)
                     /locus_tag="VQ526_002098"
     CDS             complement(370..768)
                     /locus_tag="VQ526_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2555753.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          295 a          189 c          134 g          323 t
ORIGIN      
        1 tcattatttc cattttcttt tggatttcac tctattctga aaaacttgtg tatatttact
       61 gaagctagca agtcttacct gtaaatttaa tgaaagcaac acaaaatccg agaggggaat
      121 ctcggattaa tagatagaga gtttttagtt taaataaatt gtttaaaata tcaacaacat
      181 cacttctttt cttaacctga taagtcttga ttcctaattt tggggctacg attatattgt
      241 cctcaatatc gtctagaaag acacaatttc taggttataa ctggtattta tcgatagtta
      301 ctcatataca tcagtccacc tccatactta tgtgcgagcc tctctttgta ttatacctcc
      361 atactcacct tacagattct tttggtaata atatctttgc ctaatgtaga gatagtcttg
      421 caaagaaaaa acttccttgt agccatgttt ctgataaaag tccggtgcct ggaactggta
      481 agtattgaca aaggcaaaac aacaatttcg attcttagct tcactttctg cctgttgcaa
      541 tagttttgaa ccgattcctt gccctcgcag ttcctctttt acaaacaaat actcgatttc
      601 tagccaattt ccaaaagtct ctgctatcaa acctgccagg agattgccct tttcatcttc
      661 gacataaaga ttaagtggct cactttcagc ctcttctctt tttgaacggt tataaacacg
      721 aatcagattc cctatttctt gcgatttatg tgattcctta ttttccaatc taatgaaatg
      781 aaataaaaca tgcgcaaatc gattaaggaa tttaatctaa tttctaacaa tgtcttagaa
      841 atcaaagtgt actattttaa cttcaatgca ctatacatct aatactcaat gaaaatcaaa
      901 gagcaaacta ggaaactagc cgcaggttgc tcaaaacact g
//
LOCUS       DAVBDG010000138          934 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVBDG010000138 DAVBDG010000000
VERSION     DAVBDG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.140"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..110)
                     /locus_tag="VQ526_002099"
     CDS             complement(<1..110)
                     /locus_tag="VQ526_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2555754.1"
                     /translation="MKLSYDDKVQIYELRKQGYSLEKLSNKFGINNSNLR"
     gene            352..692
                     /gene="tnpB"
                     /locus_tag="VQ526_002100"
                     /pseudo
     CDS             352..692
                     /gene="tnpB"
                     /locus_tag="VQ526_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(725..934)
                     /locus_tag="VQ526_002101"
                     /pseudo
     CDS             complement(725..934)
                     /locus_tag="VQ526_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          284 a          181 c          159 g          310 t
ORIGIN      
        1 tacctaagat tagaattgtt tatcccaaat ttatttgaaa gcttctctaa gctatatcct
       61 tgttttctaa gttcatagat ctgaacttta tcatcataac tcaatttcat aataaaaaca
      121 ccccaaaagt tagatttttt ctgtctaact tttggggggc agttcattct acacccacta
      181 ttatgcgttt ttatcatttt gaaatctact gctaatcaat gaagaagcaa agatcaaact
      241 aggcagctag ttacaagtta cgtaagcgcg tcataacaag gtatctatcc taattcccca
      301 acttccagta tactagataa aaaataatta tcggaggaaa ggtattgtac tatgataatt
      361 caactaagtg atttaggtca agttcacctt gtttgtagca agatagatat gaggcaggga
      421 atagactcat tagcctatgt agttaaaacc cactttgaat tggatccttt ctccggtcaa
      481 gtctttctct tttgtggtgg acgtaaagac cgctttaaag ccctttactg ggatggtcaa
      541 ggattttggc tactatataa acgctttgag aacggcagac tgacttggcc cagtacagaa
      601 aaggatgtca aagctctcac acctgaacaa gtagactggc ttatgaaggg cttttctatc
      661 actccaaaaa taaatccatc agaaagtcgt gatttctatt gaaatgagga ctttcttttt
      721 atactcatct gctttcaaaa agcattctag tccatctccg attaacgatg ggctttatca
      781 cctccttctc cagtccttgt atgatatctt aaagttgatt catgacatct tccaaagttt
      841 gaaaggcttt atttttaaat ccacgtttac gaatctcttt ccacacttgt tcaatggggt
      901 tcatctctgg tgtgtatgga ggaataaatg caaa
//
LOCUS       DAVBDG010000139          824 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVBDG010000139 DAVBDG010000000
VERSION     DAVBDG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.141"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(244..555)
                     /locus_tag="VQ526_002102"
     CDS             complement(244..555)
                     /locus_tag="VQ526_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555755.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(570..>824)
                     /locus_tag="VQ526_002103"
     CDS             complement(570..>824)
                     /locus_tag="VQ526_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET2555756.1"
                     /translation="IRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNP
                     MELLTIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          260 a          148 c          139 g          277 t
ORIGIN      
        1 agacggattg ttttgacaat tctaaccttt tagactttgc ataataactg ttaaacagtt
       61 gaaaattaac ccagttttaa caagtagtca aaataccaga taaaggaaaa aagtctttaa
      121 aatcttaaaa acgcatagta tcaggtattg aacaatcttg atattatgcg ttttattgtg
      181 ggaagattta cttcattttc tcttggaatt gagttttctc ctacttcaaa actagatgat
      241 ttgctattta aaatagcgtt ccttatgatt ttggcaacct ggattaaagc gagaaaaaca
      301 aaaggggcaa cttaagcttt ctttatattg cttatatgtt agtagtttta gacaaacacc
      361 acataaaata ggcttatcct gtatcaaaga taagggatac ggctcaaaag ggtgattttc
      421 taatgaatca tgacaccgat aacaagcata atacttttta caatcatagc attgtaaagc
      481 aataatatcc agcttgctat ggtaatgaat gcatcttgtt tgcttatcca ctaagaaacc
      541 ctgtgcctct atcatttcct tttcctttct taagctcgaa ctgtgacgct ggttccatct
      601 tccttataaa gattgatgag tccttctttt aatgcacgaa ccatgtctcc tgattgaacg
      661 ggttgaggca ccttaatagt caatagttcc attggatttg gagcgcggtc gattttattg
      721 cctttggcat catgcaaatc ttcaatatag gtttcaaaat gacggaaacc tggaccataa
      781 aactcaactt ggtccccttc gttaatgaca tttcgttgac gaat
//
LOCUS       DAVBDG010000140          818 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVBDG010000140 DAVBDG010000000
VERSION     DAVBDG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.142"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(171..>818)
                     /locus_tag="VQ526_002104"
     CDS             complement(171..>818)
                     /locus_tag="VQ526_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HET2555757.1"
                     /translation="KTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSETL
                     YQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIKE
                     MKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLHV
                     VGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          216 a          175 c          177 g          250 t
ORIGIN      
        1 tcaactagat gtaacttaca aaacccctga cctcatgagc cactttcttc ctcctcatga
       61 ggtcagtttt actttctgct gttccagtat cgtttttcct cgctagattt cctcaaaagg
      121 gcagactcct cccttggtgc gtcacacgat tttttcatct cgactgttct ttaatgcatc
      181 attaacgacg cttttcttct aggtggttca taaggaacag gaagattcag gttgactttt
      241 ctaatcctag aataaagtgc tgaaaacaat tcggaatagg catagagact agacaatttg
      301 aggagctgct tgcgtcctgt tcgaacacat tttcccacca cgtgaagaaa aagatggcgg
      361 aagcgtttga ttgttaaagt ttggaagtca cctccagcta gatgtttgag aaaaagatag
      421 agattgtagg cgatacagct catcatcata cgaacttcgt ttttgattaa ggttgaacta
      481 tccgttttat cgccaaaaaa tccctccttc atctccttga tgaaattctc ggcttgacca
      541 cgtccacgat aaagctgaaa ctggtcttgg cttgttccac tcgtcatatt tgtaacgaga
      601 gaaataacat cgtagaacaa gtttccttct tttcgttcag agaactggca gacacgacgc
      661 ttgtgcgacc aagatcctgc ttgatagaga gtttctgagt aggcggagtg gggcaagatg
      721 gttaagtcct catcctgtgg gcaagggagg gaaaggtctc caagacggct cagaacagta
      781 tttttcttga gttttatgag gtagtattgc cctgtttt
//
LOCUS       DAVBDG010000141          758 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVBDG010000141 DAVBDG010000000
VERSION     DAVBDG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.143"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..>758
                     /locus_tag="VQ526_002105"
     CDS             <1..>758
                     /locus_tag="VQ526_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2555758.1"
                     /translation="DGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTS
                     SIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLI
                     ASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLS
                     EQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDE
                     SVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQ"
BASE COUNT          297 a          110 c          120 g          231 t
ORIGIN      
        1 tgatggaagt actcaagaaa ttgatgttcc ttatgatatt cctgatactt gggagtgggt
       61 gaggataaaa tcaatttatt ggaattttgg gcaaaataag ccagagaaat cctttaggta
      121 tatagatacg tctagtattg atagaaaaaa gaacataatc aactacaaaa atctacaata
      181 tctttcacct gaacaagcgc cttcccgtgc cagaaaatta gtttcgcaga atagtgtctt
      241 attttcaaca gttagaccat atctaaaaaa tattgctgta gttagagaac ttaaagagta
      301 tttgatagct agtacagcat ttattgtttt ggatacttta cttaacgaaa catatttgaa
      361 atattattta ttatcagata attttatcaa tcgtgttaat aataaatcta ctggaacaag
      421 ttatcctgca atcaatgatt ataattttaa tctattatta attgctctcc ccccactatc
      481 cgaacaacaa cgaatagtag aagcaatcga atcagcttta gaaaaagtag atgaatatgc
      541 tgaaagttat aatagactag aacagctaga taaagaattt ccagataaac taaaaaaatc
      601 tattcttcaa tatgctatgc aaggaaaatt agttgaacaa gacccaaatg atgaatcagt
      661 cgaagtttta cttgaaaaaa tacgagcaga aaaacaaaaa ctctttgaag aaggcaagat
      721 taaaaagaaa gatttggaca tttctattgt ttcccaag
//
LOCUS       DAVBDG010000142          729 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVBDG010000142 DAVBDG010000000
VERSION     DAVBDG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.144"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(1..729)
                     /locus_tag="VQ526_002106"
                     /pseudo
     CDS             complement(1..729)
                     /locus_tag="VQ526_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          236 a          123 c          150 g          220 t
ORIGIN      
        1 ttagaatagt gtagttgaag ggcgttgaca atcttttctt tatctttgag gaaggtttta
       61 aagacagtct gaaaaatagg atgaacctgc ttaagattgt cctcaataag tccgaaaaat
      121 ttctctggtt ccttattctg gaaatgaaaa agcaagagct gatagagttg atagtggtgt
      181 ttcaagtctt ccgaataact caaaagcttg tttaaaatct ctttattggt tcaatgcata
      241 cgaaaagtag gacgataaaa atgtttatca ctcagtttac gactatccta ttgaatgagt
      301 ttccagtagc gcttgatagc cttgtattca tgggattttc gatgaaactg atttatgatt
      361 tgaacacgca cacgactcat agcacggcta agatgttgta caatgtgaaa gcgatccaac
      421 acgattttag cattcgggag tgaaacagtc tgggagactg tttcagcctg agcctagaaa
      481 tttgaaagcg aaactgttta gccaagtcat agtaagggct aaacatatcc atagtaatga
      541 ttttgacgcg acatcggaca actctatcgt agcgaagaaa gtgatttcga atgatagctt
      601 gtgttctacc ctcaagaaca gtgatgatat tgagattgtt aaaatcttgc gcaatgaagc
      661 tcatctttcc ctttgtaaaa gcatactcat cccaagacat aatctcagga agacaagaaa
      721 aatcatgtt
//
LOCUS       DAVBDG010000143          728 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVBDG010000143 DAVBDG010000000
VERSION     DAVBDG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.145"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..>728
                     /locus_tag="VQ526_002107"
     CDS             <1..>728
                     /locus_tag="VQ526_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2555759.1"
                     /translation="CLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHY
                     HNQKFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLPYPRKGKALKKQASTNFKP
                     AGQSIAQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAE
                     VVNQALKLILKQHKILSITADNGTEFNRLSDVFSEEHIYYAHPYASWERGTNENHNRF
                     IRRWLPKGTKKMTPKEVAFIEKWI"
BASE COUNT          240 a          148 c          159 g          181 t
ORIGIN      
        1 aatgtcttgg aaaagggcgc ttcaaagagg tttattctgc cgactacgct caacagtctt
       61 atgaaaacaa tcgcaagcgc tcggtcaaga aatcaagctt gaccaaggaa ctaaaggaaa
      121 agattctcca ctatcataac caaaaatttt cgcctgaaat gatggtgatg gctaaagggg
      181 ttaacgtggg aatttcaacc atttactatt ggattcatca tggaaaattg gggttaagca
      241 agcaggattt gccttaccct agaaaaggaa aagcgcttaa gaaacaggcc agcaccaact
      301 ttaaacctgc tggtcaatcc atcgcacagc ggcctgaagc tatcaatctt cgcttggaga
      361 atgggcatta tgagattgat acggttctac ttacgagagc gaaaaactac tgcttgcttg
      421 tcttgacgga tcgaaagagt agacatcaga tcatccgatt gattccaaat aaaagtgctg
      481 aggtggtcaa tcaggctcta aaactcatct taaaacaaca caagattctt tccatcacgg
      541 cagataatgg aacggaattc aatcgcttgt ctgatgtatt ttctgaggag cacatttatt
      601 atgcgcaccc ctatgcctct tgggaaaggg gaactaatga gaatcacaac aggttcattc
      661 gtagatggtt acctaaggga accaagaaaa tgactcccaa agaagtcgca ttcatcgaaa
      721 agtggatt
//
LOCUS       DAVBDG010000144          664 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVBDG010000144 DAVBDG010000000
VERSION     DAVBDG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.146"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            330..>664
                     /locus_tag="VQ526_002108"
     CDS             330..>664
                     /locus_tag="VQ526_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2555760.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKNLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFF"
BASE COUNT          227 a          100 c          123 g          214 t
ORIGIN      
        1 atgagttcac taatctggag atttttcaat agacttcgtt attgggcggt taggatatga
       61 tcactgactt cgtcagtctt atctacaacc tcaaaacagt gttttgagca acctgcggct
      121 agcttcctag tttactcttt gattttcatt gagtattaga acagaaaaaa tgcttggagt
      181 atttgtttgt gtgtttattt ttatataaca aactataaac aaaacaaaaa tataaaaaaa
      241 gagacaaaaa agaacagaaa gtaattgaca acggtttcat atagtgttat acttatggca
      301 taaagttaat ggaaagaagg gaaaaactta tggtattaga ttatttcttt gacaaaaacc
      361 ttgtgttttg cttagaagcg gataatcaag aacatctctt tgatcaggtt gcaactttat
      421 tggaagaacg agaaattgta actccaactt atcgtgaagc cttgatcacg cgtgaaaagt
      481 catttccaac tggtttagat atggaatttc taggaaagaa cttgccaaat gtagcgattc
      541 ctcacacaga tattgttcat aatctagctg agaaagtggt ggttgttcga ttagaaaaac
      601 cagtaacttt tcacaatatg atagctcctg ataaggaagt agaagtatcc ctactcttct
      661 ttat
//
LOCUS       DAVBDG010000145          622 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVBDG010000145 DAVBDG010000000
VERSION     DAVBDG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.147"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
BASE COUNT          222 a           63 c          115 g          222 t
ORIGIN      
        1 tacttgacag ttattatatt ttcgcttatg cttattatat agataaaagg acgggatagg
       61 agtttaaaaa tttgaaagcg ctatccataa taataacgca taattttctc cttttcgtga
      121 aaaaagttaa cgtaatttta aaatgagctt aataagaggt actatgtagg tagaaaataa
      181 agataggaga aaaagtatga ataaaaaaat attttgtatt ctggtatgta ttatatcatt
      241 gattagtcta gctataatct ttccatgggg ctggtcgata taatgtaagg gagcttgagt
      301 tgttagtgga ggataaaatg ttaagagtaa tagtagttat tcaatcttta gtaataatac
      361 tgcttgctgt tgctttatta ttatctcgga aaaactttga ttcgttaaat gttgttctta
      421 taatgatgat cgtatcacaa ttgttgttat tagtatattt gataaaatca gggaatcagt
      481 gattagctgt tatttgaatt aagtaaactg ttaagcttca agcagtaact ggttttaaat
      541 atttaaaata agataaagga aggaagtcta atttaggtaa ctaaacaaag cctgcgatca
      601 cttgttagga aactatccta ac
//
LOCUS       DAVBDG010000146          604 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVBDG010000146 DAVBDG010000000
VERSION     DAVBDG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.148"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(21..500)
                     /locus_tag="VQ526_002109"
     CDS             complement(21..500)
                     /locus_tag="VQ526_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2555761.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          161 a          140 c           81 g          222 t
ORIGIN      
        1 attttttttt gcatgactta ctagtgggta cggatagtaa actccttaaa cacaatgcgt
       61 aagtctctta atactctttg acgccctcga aatcgttcat tgcttaagac gcgttctagt
      121 tcttcttgtt tctctttact ttgtttgttt ctataatctc ttagtgtttc atgaaagaga
      181 taataatcat ctagccagag acccccctca cttatacgat gactgatctc tcccacttct
      241 tcatagggtt ctttatcgta tctacgcttc tgactttcct gcttacggat atagtctaaa
      301 attcgatttc gaaacttggt tttgaaatat ttccttaaac gtggaatatc gtctaccagt
      361 ccttcttctc tactaatcaa ttcatgtaag cagagcatgc cttcttggtc ccaatccgac
      421 aattcccata aatgaaggta atattcattt ctacacttat aaacagtccc ttggacttct
      481 tcatacaatt ctttaatcat aattttcccc tttctagata cagtctaaca gattagaaaa
      541 cacatttgtt ccattttctc cattctgttc cctttagctc ctaaactgca caaaaaagag
      601 aggc
//
LOCUS       DAVBDG010000147          598 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVBDG010000147 DAVBDG010000000
VERSION     DAVBDG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.149"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(171..244)
                     /locus_tag="VQ526_002110"
     tRNA            complement(171..244)
                     /locus_tag="VQ526_002110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(208..210),aa:Ile,seq:gat)
     gene            complement(279..349)
                     /locus_tag="VQ526_002111"
     tRNA            complement(279..349)
                     /locus_tag="VQ526_002111"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(315..317),aa:Gly,seq:tcc)
     gene            complement(355..427)
                     /locus_tag="VQ526_002112"
     tRNA            complement(355..427)
                     /locus_tag="VQ526_002112"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(392..394),aa:Val,seq:tac)
     gene            complement(432..547)
                     /gene="rrf"
                     /locus_tag="VQ526_002113"
     rRNA            complement(432..547)
                     /gene="rrf"
                     /locus_tag="VQ526_002113"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          167 a          163 c          128 g          140 t
ORIGIN      
        1 gaggatgtgg gattcgaacc cacgcacgct tttacacgcc tgacggtttt caagaccgtt
       61 cccttcagcc ggacttgggt aatcctccaa aatagtttat acgggaacaa gcccgtgtcc
      121 tcttagtgaa cagataatat tccaatcccc tagtaatgga ctaaacacta tgggcacgag
      181 tggactcgaa ccaccgacct cacgcttatc aggcgtgcgc tctaaccacc tgagctacgc
      241 gcccaagtca aaaacttggt acaaagaaca aagttcaaag cgggtgacga gaatcgaact
      301 cgcgacaaca gcttggaagg ctgtagtttt accactaaac tacacccgct aaaatgggag
      361 ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc agctgagcta
      421 aactccctag agctaagcga cttccctatc tcacaggggg caacccccaa ctacttccgg
      481 cgttctaggg cttaacttct gtgttcggta tgggtacagg tgtatctcct aggctatcgt
      541 cacttaactc tgagtaatac ctactcaaaa ttgaatatct attcaattca agaaaacc
//
LOCUS       DAVBDG010000148          589 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVBDG010000148 DAVBDG010000000
VERSION     DAVBDG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.150"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            68..589
                     /locus_tag="VQ526_002114"
                     /pseudo
     CDS             68..589
                     /locus_tag="VQ526_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT          190 a          107 c          129 g          163 t
ORIGIN      
        1 ggaaagtcaa attaatttat agaaatattt tagcagccaa ggtgtactgt tatagattca
       61 atacactata gactgtaatc aaacaacgat ttggcgaaat gtaaaaaaat atgaggagtt
      121 cggactcgac tctctccttc aagaaacacg tggtggtcgt aaccatgcat atatgacggt
      181 tgaggaagag aaagcctttc ttgcccgtca tttgaaggct gcagaggcag gagaatttgt
      241 tacaattgat gccttatttc aggcttataa aaaggagtta ggtcgttcct acacacgtga
      301 tgccttctat caactgttga agcgccatgg ttggcgaaat attatgccac gtccagaaca
      361 tcctaagaaa gcagacgctc aaaccattgt cgcgtctaaa aataaaattt caattcaaga
      421 agaaaagaaa gcgctttaaa accagtagac gttttcataa ggttcgcttg atgtaccaag
      481 atgaggctgg cttcggtaga atcagtaaac tgggatcttg ttggtctcca ataggagtag
      541 gtccacatgt ccatagtcac tatatacgag aatttcgcta ttgttatgg
//
LOCUS       DAVBDG010000149          536 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVBDG010000149 DAVBDG010000000
VERSION     DAVBDG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.151"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..534)
                     /locus_tag="VQ526_002115"
     CDS             complement(<1..534)
                     /locus_tag="VQ526_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2555762.1"
                     /translation="MEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAAEQGGAVGIR
                     ANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIEVIALDCTKR
                     ERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTLSGYTSYSPK
                     VDGPDFELIKKLCDAGVD"
BASE COUNT          141 a          133 c          103 g          159 t
ORIGIN      
        1 atctacaccg gcatcacaga gtttcttaat caattcaaaa tctggaccgt ctacttttgg
       61 actgtaggat gtgtagcctg ataaggttgt tccgacaaag tcaattcctg cttctacagc
      121 tgctagccct tcttcgaaga tactagtatc agccatcaaa agctgattag gatatttctc
      181 cttaacctga cgaatgaact cttgaatttc caaaccatcg tagcgttcac gcttggtaca
      241 atccagagca atcacctcga tgtccagttc tgccaattca tcaacttctt tcatagtagc
      301 cgtgatgaag ggttcctgag gtggataatc acgtttgata atcccaatga ttggaagttt
      361 agtgacttcc ttaatttcct tgatatcgcg aacactgttt gctcggatac cgactgctcc
      421 accttgctca gccgctttga ccagcaaggg aatcacccct cccgcttctg tataaagcgg
      481 ttcatgagga agagcctgac aagaaacgat gattccatct ttgatttgct caatca
//
LOCUS       DAVBDG010000150          500 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVBDG010000150 DAVBDG010000000
VERSION     DAVBDG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.152"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..>500
                     /locus_tag="VQ526_002116"
     CDS             <1..>500
                     /locus_tag="VQ526_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2555763.1"
                     /translation="SMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPN
                     TDYVIYNIPQLAGVALTPSLYTEMLKNPRVIGVKNSSMPVQDIQTFVSLGGEDHIVFN
                     GPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQYAINAIIGKL
                     TSAHGN"
BASE COUNT          136 a          112 c          110 g          142 t
ORIGIN      
        1 agtatggaac ttgctcgcca tgctgaaagc ttgggagtag atgctattgc aacgattcca
       61 ccaatttact tccgcttgcc agaatactca gttgccaaat actggaacga tattagttct
      121 gcagctccaa acacagacta cgtgatttac aacattcctc aattggcagg ggttgctttg
      181 actccaagcc tttacacaga aatgttgaaa aatcctcgtg ttatcggtgt taagaactct
      241 tctatgccag ttcaagatat ccaaaccttt gtcagccttg gcggggaaga ccatattgtc
      301 tttaacggtc cagatgaaca gttcctaggt ggccgcctca tgggtgctaa ggctggtatc
      361 ggtggtactt atggtgctat gccagaactc ttcttgaaac tcaatcagtt gattgcggat
      421 aaggacctag aaacagcgcg tgaattgcag tatgctatca acgcaatcat tggtaaactc
      481 acttctgctc atggaaatat
//
LOCUS       DAVBDG010000151          481 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVBDG010000151 DAVBDG010000000
VERSION     DAVBDG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.153"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            1..481
                     /locus_tag="VQ526_002117"
                     /pseudo
     CDS             1..481
                     /locus_tag="VQ526_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          185 a           81 c          104 g          111 t
ORIGIN      
        1 ataaagtctg acatgaaggc tagactaaag atagagtttc tcttgaatac ggtctcccaa
       61 gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat actattgttg
      121 agaaaccaag agggagagta cctaaaatgg gacgtaagcc aagaacgaga cctgaagaga
      181 ggacagaatt agaacgtctt caagcagaaa atgagtacct gagagcggag aatgccatcc
      241 taaaaaagtt aagagaactc cgattgaagg aggaaaaaga gaaagaagaa agatagaaat
      301 tgttcaagaa ttaatgactg agttttcgtt agatcttctt ctaaaagcca ttaaactagc
      361 tcgttggacc tactactatc acttgaaaca gttagacaaa ccagataagg accaagagct
      421 taaagctgaa attcaattca tttttatcga acacaaggga aattatactt atcgccgggt
      481 t
//
LOCUS       DAVBDG010000152          476 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVBDG010000152 DAVBDG010000000
VERSION     DAVBDG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.154"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(372..446)
                     /locus_tag="VQ526_002118"
                     /pseudo
     CDS             complement(372..446)
                     /locus_tag="VQ526_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
BASE COUNT          170 a           79 c           69 g          158 t
ORIGIN      
        1 tatgtgcttc aagtatataa aaaggcgtaa aaatacgcct atagataatg gggttgaaat
       61 aggtttattg ttgatgagat tgtagataat tcaatttttt acttccaatc gaatattcaa
      121 agcctccacc ttttctgcct gtaattgttc atcataaaat tcaatatctt caggattttc
      181 cccttgacaa cctcggcaga aatattcttc cgctcgatca ggattcaaaa atcgacaagc
      241 acaaacaaaa cagtcgccat catcatttat tgagataata tagtagattg aaataagatg
      301 taaacaaatc gattaggaaa gttaaattaa tttctagaaa tttttagcag atgtagtgta
      361 ctattctagt ctcaatttac tatggcttca aatatatctt tcgaaaaaat atttacagat
      421 gtgtaatttt gaagcttgca aaagttagcc ccaattattc acccctaatc taaaaa
//
LOCUS       DAVBDG010000153          461 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVBDG010000153 DAVBDG010000000
VERSION     DAVBDG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.155"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(1..461)
                     /locus_tag="VQ526_002119"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="VQ526_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          151 a           85 c           97 g          128 t
ORIGIN      
        1 acgactcata gcacggctaa gatgttgtac aatatgaaag cgatcaagaa cgattttagc
       61 attcgggagt gaaacagcct gggagactgt ttcagcctga gcctagaaat ttgaaagcga
      121 agctgtttag ccaagtcata gtaagggcta aacatatcca tagtaatgat tttgacgcga
      181 catcggacgg ctctatcata tttaagaaag tgatctcgga tgacagcttg tgttctacct
      241 tcaagaacag tgatgatatc gagcttttca aaatcttgcg caatgaagct catctttccc
      301 tttgtaaaag catactcatc ccaagacata atctcaggaa gacaagaaaa atcatgttta
      361 aagtgaaaat cattgagctt tcgaatgaca gttgaagttg aaatggccag ctgatgggca
      421 atatcggtca tagaattctt ttcaattaac ttttgcgcaa t
//
LOCUS       DAVBDG010000154          453 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVBDG010000154 DAVBDG010000000
VERSION     DAVBDG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.156"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..>453
                     /locus_tag="VQ526_002120"
     CDS             <1..>453
                     /locus_tag="VQ526_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET2555764.1"
                     /translation="EARFDKLIEIKRLTASKIQDILSVAPRSIGTTSPAREFEIIEII
                     KHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILAEIRNIHAFDNPAQLQAF
                     AGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACARFSPAFKAY"
BASE COUNT          119 a          101 c          102 g          131 t
ORIGIN      
        1 ttgaggcaag atttgataag ctgatagaaa ttaagcgatt gaccgcttct aaaattcagg
       61 atatcctctc agtcgcacct cgttctatcg gaacaacatc tcctgctcgt gaattcgaaa
      121 tcatcgaaat catcaaacat tacaagaggc tcattgacaa ggcggaaaca tgtgtcaatg
      181 acttgatggc tgagttcaac tcggtcatca cgacggttac tgggattggg ggtcgtttag
      241 gggcggtcat tttagccgag attcgaaata ttcatgcctt tgataatcct gctcaattac
      301 aagctttcgc tggactggat tcttctattt atcagtcagg tcagattgat ttagctggaa
      361 gaatgatcaa acggggttcc cctcatctgc ggtgggcact catacaagct gccaaagcat
      421 gcgctcgctt ttcacctgct tttaaggcct atc
//
LOCUS       DAVBDG010000155          432 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVBDG010000155 DAVBDG010000000
VERSION     DAVBDG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.157"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..>432
                     /locus_tag="VQ526_002121"
     CDS             <1..>432
                     /locus_tag="VQ526_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2555765.1"
                     /translation="GGCSQSCRWKYDLYDMPFGKERKSLQGEIPEEFSMSAVDMSMID
                     HISDMIENGVDSLKIEGRMESIHYVSTVTNCYKAAVDAYLESPEKFEAIKQDLVDEMW
                     KVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVVSY"
BASE COUNT          124 a           73 c          110 g          125 t
ORIGIN      
        1 tggtggatgt tctcagtcat gccgttggaa gtacgacctt tacgatatgc catttgggaa
       61 agaacgtaag agtttgcagg gtgagattcc agaagaattt tcaatgtcag ccgttgatat
      121 gtctatgatt gaccatatct cagatatgat tgaaaatggt gtggacagtc taaaaatcga
      181 aggacgtatg gagtctattc actatgtatc aacagtaacc aactgctaca aggcggctgt
      241 ggatgcctat cttgaaagtc ctgaaaagtt tgaagctatc aaacaagact tggtggacga
      301 gatgtggaag gttgcccaac gtgaactggc tacaggattt tactatggta caccatctga
      361 aaatgagcag ttgtttggtg ctcgtcgtaa aatccctgag tacaagtttg tcgctgaagt
      421 ggtttcttat ga
//
LOCUS       DAVBDG010000156          398 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVBDG010000156 DAVBDG010000000
VERSION     DAVBDG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.158"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            184..>398
                     /locus_tag="VQ526_002122"
     CDS             184..>398
                     /locus_tag="VQ526_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET2555766.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAA"
BASE COUNT          148 a           48 c           96 g          106 t
ORIGIN      
        1 gtaaaaaagt gattagaaaa catctttttt aaaaatagag atgattttga aacaaaaaag
       61 ctaattcaag acgtttctat gccaattcaa gatttggatg aaaaaaatta atagatactg
      121 ttatactaaa cttgtcaagt ttgtaacaag acaaatatta aaaataaaaa agaggtattc
      181 gttatgaata caaaaaagat gtcacaattt gaaattatgg atactgagat gcttgcttgc
      241 gttgaaggtg gcggatgcaa ttggggagat tttgccaaag caggtgttgg aggaggagca
      301 gtacgaggtc ttcagctagg aattaaaaca agaacatggc aaggtgcagc aactggtgct
      361 gcgggaggag ctatacttgg aggtgtggcc tatgcagc
//
LOCUS       DAVBDG010000157          340 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVBDG010000157 DAVBDG010000000
VERSION     DAVBDG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.159"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            268..340
                     /locus_tag="VQ526_002123"
                     /pseudo
     CDS             268..340
                     /locus_tag="VQ526_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          106 a           63 c           91 g           80 t
ORIGIN      
        1 tctgaggtgt aagtcctcgg tgggcacctg ttaccggtga acccaatagc gattcccaag
       61 cctgactatc gtgaggtagc ggattaaaac ggtctgggga tagaccgttt taaatctgac
      121 gctggaaata agaatcgtca gaggaaggga tagcgaactc gtggctctac gaacaggaac
      181 gtgatagtaa ggcgtatata gcggataagg gggcctcaaa ctctaaagtt caaaaaggta
      241 gtcgtaacct atatgcgtaa atcacgagag caactgaatt tgtccattcg aggatggata
      301 aactatttct cattgggaaa tatgaaaagt atagtcgcca
//
LOCUS       DAVBDG010000158          326 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVBDG010000158 DAVBDG010000000
VERSION     DAVBDG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.160"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..>326
                     /locus_tag="VQ526_002124"
     CDS             <1..>326
                     /locus_tag="VQ526_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2555767.1"
                     /translation="FDPAQITSRTANGVAVPHGDHYHFIPYSQLSPLEEKLARIIPLR
                     YRSNHWVPDSRPEQPSPQPTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYV
                     FEENGV"
BASE COUNT           98 a           85 c           63 g           80 t
ORIGIN      
        1 ttttcgaccc agcgcaaatc acaagtcgaa ccgccaatgg tgttgctgta ccgcacggag
       61 accattatca ctttattcct tattcacaac tgtcaccttt ggaagaaaaa ttggctcgta
      121 ttattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaagacca gaacaaccaa
      181 gtccacaacc gactccggaa cctagtccaa gtccgcaacc tgcaccaaat cctcaaccag
      241 ctccaagcaa tccaattgat gagaaattgg tcaaagaagc tgttcgaaaa gtaggcgatg
      301 gttatgtctt tgaggagaat ggagtt
//
LOCUS       DAVBDG010000159          297 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVBDG010000159 DAVBDG010000000
VERSION     DAVBDG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.161"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            116..>297
                     /locus_tag="VQ526_002125"
     CDS             116..>297
                     /locus_tag="VQ526_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HET2555768.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCR"
BASE COUNT           95 a           57 c           49 g           96 t
ORIGIN      
        1 cttagatcct ggatggtttc ttttttcacc caatgggtgt tttttactag acaaaaaaga
       61 gtttcccctt tatggtataa gtgtagaaaa aaacacaaaa agaaaggaaa ctcacatgaa
      121 cagtttacca aatcatcact tccaaaacaa gtctttttac caactatctt tcgatggagg
      181 tcatttaacc cagtatggtg gtcttatctt ttttcaggaa cttttttccc agttgaaact
      241 aaaagagcgg atttctaagt atttagtaac gaatgaccaa cgccgctact gtcgtta
//
LOCUS       DAVBDG010000160          289 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVBDG010000160 DAVBDG010000000
VERSION     DAVBDG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.162"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(1..227)
                     /locus_tag="VQ526_002126"
                     /pseudo
     CDS             complement(1..227)
                     /locus_tag="VQ526_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           86 a           61 c           60 g           82 t
ORIGIN      
        1 acatagccag aataatctcc taggaattct tgtactaccg aaccactccg actctgatca
       61 tgatggtaca gcgtgattgc ttgattctca actttcccag acaaaaaggt ccaatagtag
      121 gtcagatggc tatgactctc tagaacccga taagaagttt catccgcatg aagtaagggc
      181 tgagtcaata gtctctctcg caagaggtta taaaggggct ttaggaatag gagctttcac
      241 aagcttatcc agatgattat cttttactcg ttatggacaa tgctatatg
//
LOCUS       DAVBDG010000161          272 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVBDG010000161 DAVBDG010000000
VERSION     DAVBDG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.163"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
BASE COUNT           96 a           60 c           40 g           76 t
ORIGIN      
        1 cttaccatct agccatttgc agacgaaata caagataatt ccagctataa catctgctat
       61 aatagtaaga gcaagctctg gtataaggct cattagtttc acctcctctc acgaacccat
      121 aggaacgtaa tcggtaaccg atgacaaaag tattatacca cactaaattt agatcatcaa
      181 cctcacttaa tgcttgaact attgtagatt atatgctaaa atgtagttaa ttgataaggt
      241 tcattagaac accaaagccc ctaactatgg cc
//
LOCUS       DAVBDG010000162          267 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVBDG010000162 DAVBDG010000000
VERSION     DAVBDG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.164"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>267)
                     /locus_tag="VQ526_002127"
     CDS             complement(<1..>267)
                     /locus_tag="VQ526_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2555769.1"
                     /translation="ELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEGVAYNAHYRAHGY
                     HPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLL"
BASE COUNT           91 a           49 c           60 g           67 t
ORIGIN      
        1 aagagaagtt gattaaaccg ttctaaaaca ggtgtgataa agctgtctgc ctcttcagaa
       61 caataacgat taccaggacg aagctgggca ttgaaacaat aacctgtctt cccctcgaaa
      121 gcataaagag gatgatagcc atgagcacga tagtgggcgt tataagcaac accttcttgc
      181 ttgccataag ttgtgaaatg ggtagaatcg atatctacaa tgagttggtt tagctggtga
      241 aactgtaaaa agaattcgac caattca
//
LOCUS       DAVBDG010000163          263 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVBDG010000163 DAVBDG010000000
VERSION     DAVBDG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.165"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            1..73
                     /locus_tag="VQ526_002128"
     tRNA            1..73
                     /locus_tag="VQ526_002128"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Met,seq:cat)
     gene            87..160
                     /locus_tag="VQ526_002129"
     tRNA            87..160
                     /locus_tag="VQ526_002129"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Ile,seq:cat)
     gene            171..260
                     /locus_tag="VQ526_002130"
     tRNA            171..260
                     /locus_tag="VQ526_002130"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:207..209,aa:Ser,seq:tga)
BASE COUNT           53 a           66 c           76 g           68 t
ORIGIN      
        1 gcggtgtagc tcagctggct agagcgtccg gttcataccc gggaggtcgg gggttcgatc
       61 cccttcgccg ctataatgat cttgtcggac ctttagctca gctggttaga gctctcggct
      121 cataaccgag tggtcgtagg ttcaagtcct acaaggtcca tttgaatatt ggaggattac
      181 ccaagtccgg ctgaagggaa cggtcttgaa aaccgtcagg cgtgtaaaag cgtgcgtggg
      241 ttcgaatccc acatcctcct ttt
//
LOCUS       DAVBDG010000164          251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVBDG010000164 DAVBDG010000000
VERSION     DAVBDG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.166"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..>251
                     /locus_tag="VQ526_002131"
     CDS             <1..>251
                     /locus_tag="VQ526_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2555770.1"
                     /translation="PIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDI
                     TSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKG"
BASE COUNT           84 a           51 c           60 g           56 t
ORIGIN      
        1 ccgattcgtc atccagaacg tttaggaaaa ccaaatgcgc aaattaccta cactgatgat
       61 gagattcaag tagccaagtt ggcaggcaag tacacaacag aagacggtta tatctttgat
      121 cctcgtgata taaccagtga tgagggggat gcctatgtaa ctccacatat gacccatagc
      181 cactggatta aaaaagatag tttgtctgaa gctgagagag cggcagccca ggcttatgct
      241 aaagagaaag g
//
LOCUS       DAVBDG010000165          224 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVBDG010000165 DAVBDG010000000
VERSION     DAVBDG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.167"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>224)
                     /locus_tag="VQ526_002132"
     CDS             complement(<1..>224)
                     /locus_tag="VQ526_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2555771.1"
                     /translation="EGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGLYVNGSSG
                     ECIYQSVEDRKLILEEVMAVAKGKLTIIAHV"
BASE COUNT           67 a           55 c           40 g           62 t
ORIGIN      
        1 acatgggcaa taatggtcaa tttacctttg gctaccgcca tgacttcttc caaaatcaac
       61 ttgcgatctt caacgctttg gtagatacat tcaccagaag aaccattgac ataaagacct
      121 tgaacacctt tatcaatgaa gtattgaacc aaggcacgcg tacgttctgg gcttacttct
      181 ccttgatcat cataacatgc gtagaaggct ggaatgacac cttc
//
LOCUS       DAVBDG010000166          206 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVBDG010000166 DAVBDG010000000
VERSION     DAVBDG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.168"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            25..>206
                     /locus_tag="VQ526_002133"
     CDS             25..>206
                     /locus_tag="VQ526_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET2555772.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSK"
BASE COUNT           81 a           39 c           30 g           56 t
ORIGIN      
        1 aaacaactca ttagaaagat tcatatggaa caattacatt ttatcacaaa actgctcgat
       61 attaaagacc caaacatcaa gattctagat atcatcaata tggataccca caaagaaatt
      121 atcgctaagc tggattatga ggctccatct tgccctgatt gtggaagtct aatgaagaaa
      181 tatgactttc aaaaaccgtc taagat
//
LOCUS       DAVBDG010000167          200 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVBDG010000167 DAVBDG010000000
VERSION     DAVBDG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.denovo.169"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
BASE COUNT           81 a           49 c           30 g           40 t
ORIGIN      
        1 gaaatctgtc aaattcaaga acctatcgcc gacaaaatag cgataacact tcaagaacaa
       61 actgggtacc ttctgtaagc aatccaggaa ctacaaatac taacacaagc aacaacagca
      121 acactaacag tcaagcaagt caaagtaatg acattgatag tctcttgaaa cagctctaca
      181 aactgccttt gagtcaacgc
//
LOCUS       DAVBDG010000168        18170 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.guided.039, whole genome shotgun sequence.
ACCESSION   DAVBDG010000168 DAVBDG010000000
VERSION     DAVBDG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.guided.039"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..564
                     /locus_tag="VQ526_002135"
     CDS             <1..564
                     /locus_tag="VQ526_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555773.1"
                     /translation="KEQVKDLPTNARYAVYQGKSKVKENVRDLTSSISQTKADRASGR
                     KEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQYHDEQTSKQSNIQTSYK
                     ESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTTADRTSQRPITKERPST
                     VQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            561..1562
                     /locus_tag="VQ526_002136"
     CDS             561..1562
                     /locus_tag="VQ526_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HET2555774.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            1559..2491
                     /locus_tag="VQ526_002137"
     CDS             1559..2491
                     /locus_tag="VQ526_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET2555775.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            2736..2852
                     /locus_tag="VQ526_002138"
     CDS             2736..2852
                     /locus_tag="VQ526_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HET2555776.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            2868..4787
                     /gene="tet(M)"
                     /locus_tag="VQ526_002139"
     CDS             2868..4787
                     /gene="tet(M)"
                     /locus_tag="VQ526_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HET2555777.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            4906..5073
                     /locus_tag="VQ526_002140"
     CDS             4906..5073
                     /locus_tag="VQ526_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HET2555778.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(5133..5486)
                     /locus_tag="VQ526_002141"
     CDS             complement(5133..5486)
                     /locus_tag="VQ526_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2555779.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            5991..6413
                     /locus_tag="VQ526_002142"
     CDS             5991..6413
                     /locus_tag="VQ526_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2555780.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYQHRTSGLALIKKFMEEFEE"
     gene            6410..6640
                     /locus_tag="VQ526_002143"
     CDS             6410..6640
                     /locus_tag="VQ526_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2555781.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            7101..7304
                     /locus_tag="VQ526_002144"
     CDS             7101..7304
                     /locus_tag="VQ526_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HET2555782.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            7386..8603
                     /locus_tag="VQ526_002145"
     CDS             7386..8603
                     /locus_tag="VQ526_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2555783.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(9008..11821)
                     /locus_tag="VQ526_002146"
     CDS             complement(9008..11821)
                     /locus_tag="VQ526_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2555784.1"
                     /translation="MKDKREIIRARKAFRRSLKDEKKFLKQGKKEVRKQKKDSAVLDE
                     KAWKNEIKQKLENMREASKARVKQANEDYNHILQNSPPSLLNRKELRDRRLPHARKRL
                     KIAKKQFKEAKVEAKEERKESRKERKTNQKFFYGQETKVRSNFFLQGKNLEELKAKKE
                     VKAAKENLKSTKQAYKFKKISRKAKTFLYVLGREGGELASENEDLESYRTLHETIRKG
                     KRYSRLSYNLGKASVKTGQATGRFTKKRLTNTKERYHHFKDGKGWKLTKDNPSSFKNR
                     YRKLKKQGLLSVRNIYQKLKAAFSFFTFVAGNPVTWIVGGIVFLLLLMMSFFLGFSSA
                     SLIQQDEFELTKAYTHLTWEDAEHTRTNDKGITYYTKVDDVMGYMNFKFHDYELHKPV
                     HLFSSESYKDYLSTLWHDLNDGDDLKSMQDLYETPKYKLSRDDQEEIKELKEEGVYAS
                     MQELDNPFEGKSNEDSLTMTYRYGYYDLDGKPTLQEYILLEAKAHQTIVAPMDGVVSL
                     DGDDVILTNGKGENESRLTLYSIHNGRAIEGTRVLTGDIIGETPDDTGLKVSYQKYKN
                     KKEKLVYVNPQFYFPKVIQLQTTILPAIGQFGGDEFERAKHIYEFLKSQGASPQAIAA
                     ILGNWSVESSINPKRAEGDYLSPPVGATDSSWDDESWLAIGGPAIYSGAYPNILHRGL
                     GLGQWTDTADGSTRHTALLNYARTQNKKWYDLDLQLDFMLHGDSPYYQSWLKDFFKNT
                     GSAANLAQLFLTYWEGNSGDKLLERQTRATEWYYQIEKGFSQTNGGQAKSDPQSLEGV
                     RGDLYDHSVPGGGDGMAYAYGQCTWGVAARMNQLGLKLKGRNGEKISIINTMGNGQDW
                     VATASSLGGETGSTPRAGAIVSFVGGTHGTPASYGHVAFVEKVYDDGSFLVSETNYGG
                     NPNYTFRKISQADSAISFAYTTK"
     gene            complement(11833..14190)
                     /locus_tag="VQ526_002147"
     CDS             complement(11833..14190)
                     /locus_tag="VQ526_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2555785.1"
                     /translation="MKRKSNTLKKQKTSMTNKKEEVKVKKEEVLPSTANTLSYQALYQ
                     NGLMQVKEDYFSQSYLLGDVNYQTVGLEDKGAIIEKYSDLINSLDDKTNFQLTIFNKR
                     LNLEKFRQSVLYEEKEDGYDSYRKELNRMMNQNLDSGENNFSAVKLISFGRKDSNPKQ
                     AYRSLSQIGEYFKSGFSEIDARFESLAGEERVNLLADMLRGEHHLPFSYRDLTKSGQT
                     TRHFIAPNLLDFKNKNYLQINDRLLQIVYVRDYGMELGDQFIRDLMQGDLELIVSLHA
                     QSSTKADAMKKLRTKKTLMESQKIGEQQKLARTGIYLEKVGHVLESNIDEAEELLKTM
                     TETGDKLFQTVFLIGVFGQDEEELKQALDTIQQVAGSNDLMIDKLPYMQEAAFNSLLP
                     FGCDFLEGVSRSLLTSNIAVNSPWTSVDLQDRSGKYYGINQISSNIITIDRSLLNTPS
                     GLILGTSGAGKGMATKHEIITTKIKESGENTEIIIVDPEAEYSVIGRAFGGEMIDIAP
                     DSQTYLNVLDLSEENMDEDPVKVKSEFLLSFIGKLLDRKMDGREKSIIDRVTRLTYQS
                     FKEPSLEEWVFVLSQQPEEEAQNLALDMELYVEGSLDIFSHKTNIQTGSNFLIYNVKK
                     LGDELKQIALMVVFDQIWNRVVRNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRV
                     RKYGASPTGITQNVETLLLDPNGRRIIANSEFMILLKQAKNDREELVQLLGLSKELEK
                     YLVNPEKGAGLIKAGSVVVPFKNKIPQGTQLFDIMSTDPDKMASN"
     gene            complement(14141..14500)
                     /locus_tag="VQ526_002148"
     CDS             complement(14141..14500)
                     /locus_tag="VQ526_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HET2555786.1"
                     /translation="MNTRVFKDISKYQHRAWLGFTTRQIIFVLPAFIVTIIVLGLNLF
                     FWQFGDWFVYGFVFAFTIPLMLFGVYKPNDLYFEHYLKYRLHFELTVPLRTITGKKGP
                     EHEKKIKYIKETKNFND"
     gene            complement(14554..15408)
                     /locus_tag="VQ526_002149"
     CDS             complement(14554..15408)
                     /locus_tag="VQ526_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HET2555787.1"
                     /translation="MNLSLVSPFVYLASEKISAENLFEGFNVDLQSTVDLIKSLSSYN
                     PTVWTYMSSITKSVMQPLGVAILSVVLILEFSKMAKKIANSGGAMTFEALAPMLISYI
                     MVAVVITNTTVIVEAIIGIASHAIEQVASIVAHGGAKYDTISGLKGSGFIGRMIVGFF
                     ALLIWLVRIVSAAMVNLLVSIRFIQLYLMIPFAPLTIPTFLSDEWKSIGIGYLKNIMV
                     YAVQGVLIFLIVSLVPLFESAGKIAVSNGAGVLQSLAIMFGSLVQAILLIIALVGSQR
                     TARSILGM"
     gene            complement(15425..15667)
                     /locus_tag="VQ526_002150"
     CDS             complement(15425..15667)
                     /locus_tag="VQ526_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555788.1"
                     /translation="MITHFKGFVYGVDASAMFAQAMSLLQKGLIAVGAFLVVVGIVNL
                     ATNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQISI"
     gene            complement(15688..17565)
                     /locus_tag="VQ526_002151"
     CDS             complement(15688..17565)
                     /locus_tag="VQ526_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HET2555789.1"
                     /translation="MYSGKKFLLFSLLGILLGYLFHRLTLLYDSYTGNTLDKWTHLLM
                     EGQDQVLQSPWNVSFTGKSSVFFLLGFVMMLLVYLYLETGKKQYREGVEYGSARFGTL
                     KEKKLFYGKEFSHDTILAQDVRLTLLDKKPPQYDRNKNIVVIGGSGSGKTFRFVKPNL
                     IQMNSSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMKNSDGFNPFRYIETENDLN
                     RMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQK
                     EYQNLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYG
                     TENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGKEKS
                     MVYLVIPDNDSTFRFLSALFFSTVFQTLTRQADIDFKGQLPLHVRFYLDEFPNIGEIP
                     DFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLVYLGGNDEDTFK
                     FMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHECLVRIANM
                     PVFKSKKYNSTKHPNWKYLANQETDERWWNYQINPLNQRQENHLEGLRIRDLTFESSL
                     K"
     gene            complement(17565..18053)
                     /locus_tag="VQ526_002152"
     CDS             complement(17565..18053)
                     /locus_tag="VQ526_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555790.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYSIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     TKDRSILDNHLDHLLEKMVNNPEKLADFTMDKSLDDAIDEAKSQITFRQEGAVKQKEM
                     VR"
BASE COUNT         5745 a         3474 c         3343 g         5608 t
ORIGIN      
        1 aaagaacagg ttaaagattt gccgaccaat gcaagatatg cagtatatca aggaaaatcc
       61 aaagtaaaag agaatgtccg tgatttaacc agtagtattt ctcaaaccaa agcggacaga
      121 gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa ccattgcgaa gcgtcgctct
      181 gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt ctgttcatga aagaccgact
      241 acaagacaag aacaatatca tgatgaacag acctcaaaac agtctaatat tcagacttca
      301 tataaggaat ctcaacaagc caaacaagag cgtccagcag ttaagtccga tttttcaagt
      361 ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa ccgttcaaaa gccagcaact
      421 tcaaccacta cagcagatag aacttcacaa cgtccaatca caaaagaacg tccgtctact
      481 gttcaaagag taccactaca aaatacaaga agtagaccac caatcaaaac cgccaccatt
      541 aagaaagtcg gtaagaaacc atgaagttga aaactttagt gattggtggt tctggattat
      601 tcttgatggt cttctcactg cttctgtttg ttgccatttt attttcagat gaacaggaca
      661 gcggaatttc caatattcat tatggaggtg tgaatgtttc cgcagaagtg ctggctcata
      721 agcctatggt agaaaaatat gccaaagaat atggcgttga agaatatgtc aacatacttc
      781 ttgcgattat acaggtggaa tcgggcggta ctgcggaaga tgttatgcag tcctcggaat
      841 ccctcggtct tccacctaat tcattgagta cagaagaatc cattaagcaa ggtgtgaagt
      901 atttcagtga attattagcc agtagcgaaa ggctcagtgt agatttagaa tcggttatcc
      961 agtcctacaa ttatggtggt ggtttcttag ggtatgtggc taatcgtgga aataaatata
     1021 cctttgaact ggctcaaagt ttctcaaaag agtattcagg tggcgaaaaa gtgtcttacc
     1081 ccaatcccat agccatacct atcaatgggg gctggcgata caactatggc aatatgtttt
     1141 atgtgcaact ggtaacgcag tatcttgtca caacagagtt tgatgatgat acggtacaag
     1201 ccatcatgga cgaagcactg aaatatgagg gctggcgata cgtttacggt ggagcttccc
     1261 cgactacttc ttttgattgt agcggactga cacaatggac gtatggaaaa gctggaatta
     1321 acttaccacg aaccgcacaa cagcaatatg atgtgaccca gcatatccca ctatcggaag
     1381 cacaagctgg cgatttggtt ttctttcatt ctacctataa cgctggctct tatattactc
     1441 atgttgggat ataccttggc aataaccgta tgtttcatgc aggcgaccca atcggttatg
     1501 ccgacttaac aagcccctac tggcaacagc atttagtggg agcaggacga atcaaacaat
     1561 gagaaaggaa gatttaatga tgaaatttag aaaaaatcag aataaagaaa aacagatacc
     1621 aaaggaaaag aaacctcgtg tctataaggt caatcctcat aaaaaggttg tgattgcctt
     1681 gtgggtactt ttagggctta gtttcagctt tgcgatattc aagcacttta cagctataga
     1741 tactcatact attcacgaaa caactatcat agaaaaggaa tacgttgata ctcatcatgt
     1801 agaaaatttt gtagagaact ttgcgaaagt ctactattca tgggagcaat ccgataagtc
     1861 cattgataat cgaatggaaa gtctaaaagg ctatctgaca gatgaacttc aagctctcaa
     1921 tgttgataca gtacgcaaag atattcctgt atcgtcttct gtaagaggat ttcagatatg
     1981 gacggtagag ccaactggcg acaatgagtt taatgtaacc tacagtgtag accagctcat
     2041 tacagaggga gaaaatacaa agaccgtcca ctctgcttat atagtgagtg tctatgtaga
     2101 tggttctgga aatatggtac tggttaagaa tccgaccatt accaacatac ctaagaaatc
     2161 aagttataaa ccaaaagcca ttgaaagtga ggggacggtt gattccatta caaccaatga
     2221 aatcaatgag tttttaacga cgttcttcaa gctctatcct acagcgacag ccagtgaact
     2281 ttcctactat gtgaatgacg ggatattaaa accaatcgga aaagagtaca tctttcaaga
     2341 actggtaaat cctattcaca atcgtaagga taatcaagtc acggtatcgc tgacagtgga
     2401 gtatatcgac cagcagacca aagcaacgca ggtatctcaa tttgatttgg tacttgaaaa
     2461 gaacgggagt aattggaaga ttatagaata acaaatattg gtacattatt acagctattt
     2521 tgtaatcacg tactctcttt gataaaaaat tggagattcc ttgacaaata tgctcttacg
     2581 tgctattatt taagtatcta tttaaaagga gttaataaat atgcggcaag gtattcttaa
     2641 ataaactgtc aatttgatag tgggaacaaa taattggatg tcctttttta ggagggctta
     2701 gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat ccggagaata
     2761 tctgtatgct ttgtatgcct atggttatgc ataaaaatcc cagtgataaa agtatttatc
     2821 actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta
     2881 atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat
     2941 ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata
     3001 atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt
     3061 gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag
     3121 tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg
     3181 tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct
     3241 tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta
     3301 aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt
     3361 gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc
     3421 ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa
     3481 gcataagatt tcataattgt tccctgttcc ctgtttatca cggaagtgca aaaaacaata
     3541 tagggattga taaccttata gaagtgatta cgaataaatt ttattcatca acacatcgag
     3601 gtcagtctga actttgcgga aaagttttca aaattgagta ttcggaaaaa agacagcgtc
     3661 ttgcatatat acgtctttat agtggcgtac tgcatttgcg agattcggtt agaatatcgg
     3721 aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa
     3781 tcgataaggc ttattccggg gaaattgtta ttttgcagaa tgagtttttg aagttaaata
     3841 gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat cccctccctc
     3901 tgctgcaaac gactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac
     3961 ttttagaaat ctccgacagt gacccgcttc tgcgatatta tgtggattct gcgacacatg
     4021 aaatcatact ttctttctta gggaaagtac aaatggaagt gacttgtgct ctgctgcaag
     4081 aaaagtatca tgtggagata gaaataaaag agcctacagt catttatatg gaaagaccgt
     4141 taaaaaaagc agagtatacc attcacatcg aagttccacc gaatcctttc tgggcttcca
     4201 ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt
     4261 ctcttggata cttaaatcaa tcatttcaaa atgcagttat ggaagggata cgctatggct
     4321 gtgaacaagg attgtatggt tggaatgtga cggactgtaa aatctgtttt aagtatggct
     4381 tatactatag ccctgttagt accccagcag attttcggat gcttgctcct attgtattgg
     4441 aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt tttaaaattt
     4501 atgcgccaca ggaatatctt tcacgagcat acaccgatgc tcctaaatat tgtgcgaaca
     4561 tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc cctgctcggt
     4621 gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt gtttgtttaa
     4681 cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa
     4741 atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt attttatgtt
     4801 gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa agatttgaat
     4861 taaagtgtaa aggaggagat agttattata aactacaagt ggatattgtg tcctgtatgt
     4921 ggaaataaaa cacgattaaa gataagggaa gatactgaat taaaaaaatt ccccctctat
     4981 tgtccgaaat gcagacaaga aaatttaatt gaaataaagc agttcaaagt aactgtgatt
     5041 acagagccag acgcaaagac gcagagccga taaaatgaga ttaatacaat ctcattttat
     5101 cggctctttc cgttatgtat ggattctttt aattagtctt cgatgtttct tgcttcgttg
     5161 ataccgctgg ctaaagattc cattaaggat agttctttgt ctgtaaagct atccatgtat
     5221 ttctctatct gtaatcgtcg ggtgcttttt accaagttat tagcaggtaa gaaaaattca
     5281 tcaacggaaa catgaagtaa cgatacaagg tcataaagaa cttgtatgct ggggtgttgc
     5341 cctttatttt caatattagt taagtaccgt gggtcaattt caatcaatgc tcccacttgt
     5401 tcacgagtta aacctcgttt caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa
     5461 tcatatttat cttctttttt acgcatagta gaccacctct atacatttta ttgttcctac
     5521 tgaattaaaa acaggtatag aaaaacgtgt tatatggttt ataggtttat atttaataaa
     5581 aagcactact aaacgccaat aaaaaaaacc gttatatggt agtgctattt acgctgttaa
     5641 aatattgtat attacttcca aatggcggtt tgttggaggt caacgtcgcc atgaagtaca
     5701 tcatatacaa taaatttcct tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat
     5761 agataagtac gtccaccaat gtggttttat aaatcatata gatagaataa cagaagcatg
     5821 taaacagaga aataaatctg tttatatgct tttttggcta ttcagaactt ttttacaaag
     5881 tttatttatc agtaatgcaa caaatccccc tttcacattg ggactaagag tgaaaggaga
     5941 taaacgagca aggctcactt cctttcctag acagaaaggg ggtgagaaac atgaaaccat
     6001 cttcttttca gaccacaata gaaaatcagt ttgactatat ctgtaaacgt gctatggaag
     6061 acgagcgaaa gaattatatg ctttatcttt caaggattgc aaagcgtgag gtgtcctttt
     6121 cggatgttgg cgattatctt gttagccagt ttgcgacaac agataactat tcaactgact
     6181 ttcagatttt tacactcaat gggttatcag taggcgttga aaatgatttg ttgagtgaag
     6241 cattacgtga gttgccagac aagaaacgtg aaattctact gctgttttac tttatggaca
     6301 tgagcgattc agaaattgca gacctgttga aattgaaccg ttctactgtc tatcagcata
     6361 gaaccagtgg actagcctta attaaaaagt ttatggagga atttgaagaa tgaaaacaca
     6421 atatcctatg attccctttc ctctcattgt aaaggcaaca gatggcgata ccgaagcgat
     6481 taaccagatt ctacatcatt acagagggta cataacgaag cgttccctac gacttatgaa
     6541 agatgaatat ggcaatcaaa gtatggtcgt tgatgaagtc ttacgtggaa gaatggaaac
     6601 cagactgatt acaaagattt tgtcatttga aattaagtaa tatcctctct cctttcgtgg
     6661 aagcgtgcta aaccattcca cgcttcccga acagggaggt ttgttattcc accaaagcat
     6721 attgagcttt caatgtgttt tgataggcta acgagccatt gttctttgaa aactgaataa
     6781 aagtaatcga atacgtttcg ataagaaaag agccaacgga actaaccgcc atgacctatc
     6841 ttataaagat agcgagcgat tcatgttagt gatccgagaa gcaatcttta gcaggattgc
     6901 ctgcaacgac attcttatcg tgataatgat actcccatac agtcaatagt ccgagcgtga
     6961 taaaaccgtc gcaggcaatg agtatggcta catgagaacc atgcaggggt ggaactcccg
     7021 tgagctttgc taaagctgtt cgattgctgg taaaacaact tttatgaaat ccaaataagt
     7081 gatttggaaa ggaggatttt atgaagcaga ctgacattcc tatttgggaa cgttataccc
     7141 taaccattga agaagcgtca aaatattttc gtattggcga aaacaagcta cgacgcttgg
     7201 cagaggaaaa taaaaatgca aattggctga ttatgaatgg caatcgtatt cagattaaac
     7261 gaaaacaatt tgaaaaaatt atagatacat tggacgcaat ctagcgtcgc caaagggtct
     7321 tgtatatgat aaaatagtat taagtcgtat caaggctctt tccataaagg aaaggagcaa
     7381 atgccatgtc agaaaaaaga cgtgacaata aaggtcgaat cttaaagact ggagagagcc
     7441 aacgaaaaga cggaagatac ttatacaaat atatagattc atttggagaa ccgcaatttg
     7501 tttactcgtg gaaacttgtg gctacagacc gagtaccagc aggaaagcgt gattgtatct
     7561 cacttagaga gaaaatcgca gagttacaga aagacattca tgatggtatt gatgttgtag
     7621 gaaagaaaat gacactctgc cagctttacg caaaacagaa cgctcaaaga ccaaaggtta
     7681 gaaaaaacac tgaaactgga cgcaaatatc ttatggatat tttgaagaaa gacaagttag
     7741 gtgtaagaag tattgacagt attaagccat cagacgctaa agaatgggct attagaatga
     7801 gtgaaaatgg ttatgcttat caaaccatca ataactacaa acgttcttta aaggcttcat
     7861 tctatattgc tatacaagat gattgtgttc ggaagaatcc atttgacttt caactgaaag
     7921 cagttcttga tgatgatact gtccctaaga ccgtactaac agaagaacag gaagaaaaac
     7981 tgttagcctt tgcaaaagct gataaaacct acagcaaaaa ttatgatgaa attctgatac
     8041 tcttaaaaac aggtcttcgt atttcagagt ttggtggttt gacacttcca gatttagatt
     8101 ttgagaatcg tcttgtcaat atagaccatc agctattgag agatactgaa attgggtact
     8161 acattgaaac accaaagacc aaaagtggcg aacgtcaagt tcctatggtt gaagaagcct
     8221 atcaagcatt taagcgagtg ttagcgaatc gaaagaatga taagcgtgtt gagattgatg
     8281 gatatagtga tttcctcttt cttaatagaa agaactatcc aaaagtggca agtgattaca
     8341 acggcatgat gaaaggtctt gttaagaaat acaataagta taacgaggat aaattgccac
     8401 acatcactcc acatagtttg cgacatacat tctgtaccaa ctatgcaaat gcaggaatga
     8461 atccaaaggc attacagtac attatgggac atgctaatat agccatgacg ctgaactatt
     8521 acgcacatgc aacattcgat tctgcaatgg cagaaatgaa acgcttgaat aaagagaagc
     8581 aacaggagcg tcttgttgct tagtagtaca aatgaattta ctacttattt accacttctg
     8641 acagctaaga catgaggaaa tatgcaaaga aacgtgaagt atcttcctac agtaaaaata
     8701 ctcgaaagca catagaataa ggctttacga gcatttaaga aaatataaaa agataattag
     8761 aaatttatac tttgtttcaa ataaaaaaga tgacttactg aacctgtaag ccacctaatc
     8821 agtcggttta ttctggtgtc tgccaccgct tggcccgtac gtccaagatt gctatcggat
     8881 tttgttctgg tgtccgccac cgcttggccc ttatgtccaa gattactatc agattgacca
     8941 tgagccgacc actcatttat agacatattg tatctttaaa tagctacaat ttcaagtgta
     9001 aactcttcta tttggtcgta taagcaaaac tgatggcact atctgcttga gagatttttc
     9061 taaaggtata gttagggttg cccccatagt tggtttcaga cacaaggaaa gaaccatcat
     9121 catagacctt ctctacaaaa gccacatgac catagctggc tggtgtacca tgtgtacctc
     9181 ctacaaaaga aacaatagca cctgctcttg gtgtagagcc cgtttcccca ccaagacttg
     9241 aagctgtcgc aacccagtct tgaccatttc ccatggtatt aatgattgaa atcttttctc
     9301 catttctacc ttttaatttt aagcctaact ggttcatacg agccgcaaca ccccatgtac
     9361 attgtccata agcataggcc ataccatctc caccaccagg aacagaatga tcatacaagt
     9421 ccccacgaac cccttcaagg gattgtggat cactttttgc ctgtcctcca tttgtttgac
     9481 taaagccttt ttcaatttgg taataccatt ccgttgctct ggtttgtctt tccagtagtt
     9541 tgtcaccaga atttccctcc caataggtca gaaagagttg ggccagattg gctgcactgc
     9601 ctgtattttt aaagaaatcc tttaaccaac tttgatagta aggactatcc ccatgaagca
     9661 taaaatcaag ttgtaggtct aaatcatacc atttcttatt ttgggtgcgt gcataattta
     9721 acaaggctgt atgccgtgtt gacccatctg cggtatctgt ccattgccct aaacctagac
     9781 ctctatgaag aatattagga taagcaccac tataaatggc tggacctcca atcgctaacc
     9841 agctttcatc atcccatgag gaatcggtag cgccaacagg aggagataaa taatctcctt
     9901 cagctcgttt aggattaata gaagactcta ccgaccaatt tcctaaaatt gccgcaatgg
     9961 cttggggact tgccccttga gatttcaaaa actcataaat atgttttgct cgttcaaact
    10021 catccccacc aaactgacca atggctggta agatagtggt ctgaagttga atgacttttg
    10081 gaaaataaaa ttgcggattg acatacacca atttttcttt cttgttttta tacttttgat
    10141 aggaaacttt caaacctgta tcgtctggtg tttcaccaat aatatcaccc gttaggactc
    10201 ttgtcccctc aatcgcacgg ccattatgaa tagaatacaa ggtcaatcga ctctcattct
    10261 ctccttttcc gttagtgaga ataacatcat ctccgtctag agatacaaca ccatccattg
    10321 gtgcgacaat tgtttggtga gccttcgctt ctagtagaat atactcctga agagtaggtt
    10381 ttccgtctaa atcatagtat ccataacgat aagtcatggt tagactatct tcgttgcttt
    10441 tcccctcaaa tggattgtcc aattcctgca tggaagcata gacaccctct tcttttagtt
    10501 cctttatttc ctcttgatcg tctctcgata gtttatactt aggagtttca tagaggtctt
    10561 gcatggattt caaatcatcc ccatcgttta aatcatgcca caaagtagac agataatcct
    10621 tgtaagattc tgaactaaat aagtgaactg gtttgtgtaa ctcatagtca tggaatttaa
    10681 agttcatata ccccatcaca tcatcaactt ttgtgtaata agtaattcct ttgtcatttg
    10741 tgcgagtatg ttctgcatct tcccaagtta ggtgggtata agcttttgtt aattcaaatt
    10801 catcttgttg aatcaaacta gcagatgaaa atcctaaaaa gaagctcatc ataagtaaaa
    10861 gaagaaagac tattcctcca actatccagg ttacaggatt tccagccaca aatgtaaaga
    10921 aggaaaaggc tgcttttaat ttttgataga tatttcggac acttaaaaga ccttgtttct
    10981 ttaattttcg ataccgattt ttaaaggaac ttggattatc tttagttagt ttccatcctt
    11041 ttccatcctt aaaatgatgg tatcgctctt ttgtgttggt cagtcttttc ttggtaaaac
    11101 gacctgttgc ttgtcctgtt ttgacactag cttttccaag attataagaa agccgactgt
    11161 agcgtttccc ttttctaatg gtctcatgaa gtgtgcgata actttctaaa tcttcatttt
    11221 ctgaagctaa ctctccacct tcacgtccaa ggacataaag aaaggttttg gctttcctac
    11281 tgattttttt gaacttatag gcttgcttag tagattttag attctctttt gcggctttga
    11341 cttctttctt agcttttaat tcttctagat tcttcccttg aaggaaaaaa ttggatctta
    11401 ctttcgtttc ctgaccgtag aaaaattttt gattggtttt tctttcttta cgactctctt
    11461 ttctttcttc ttttgcttca accttagctt ccttaaattg tttcttggct attttcaatc
    11521 gtttcctagc atgaggcaat cgtctatctc ttaattcttt ccgattcaaa agtgatggag
    11581 gactattttg aagaatatga ttgtagtctt catttgcttg ttttactcta gcctttgaag
    11641 cttctctcat attctccagc ttttgcttta tctcattttt ccatgctttt tcatccagta
    11701 cagcggaatc ttttttctgt tttctcacct ccttctttcc ttgttttaag aatttcttct
    11761 catcttttag acttcttcta aatgcctttc gggcacgtat gatttctctc ttatccttca
    11821 tttaccttcc ccttaattag aagccatttt atcaggatct gtactcatga tatcaaacaa
    11881 ttgtgtacct tggggaatct tatttttaaa gggaacgaca actgaaccag cttttattag
    11941 tcctgcccct ttttctggat tgactaggta tttttcgagt tcttttgaca agcctaagag
    12001 ttgaactagt tcttctcggt cattttttgc ttgcttgagg agaatcataa attcactatt
    12061 tgcaataatc cgtctaccat ttggatctaa caataaggtt tcgacatttt gggttatccc
    12121 agtcggactg gctccatatt ttctgacacg actccacaat ttaaagaaga aatcactggc
    12181 atatttatct aataagagaa gctgcatttc atcaaaataa atccaggtct tcttccctaa
    12241 tttttggttc cgaacaacac gattccatat ctgatcaaaa accaccataa gggcgatttg
    12301 tttcagctca tctcctaact ttttaacatt atagattaag aaattagatc ctgtctgaat
    12361 attggtctta tgagaaaaaa tatcaagaga accttcgaca tacagttcca tatcaagtgc
    12421 taaattctgc gcttcttctt ctggttgttg actcaagaca aagacccatt cttccaaaga
    12481 aggctcttta aatgactgat aggtaagtct ggtaactcgg tcaataatcg atttttctct
    12541 cccatccatt tttctatcca ataacttgcc gataaaggat aaaagaaatt ctgattttac
    12601 ctttacagga tcttcatcca tattttcttc agacaagtca aggacattga gataggtttg
    12661 ggaatcaggc gcaatatcaa tcatttctcc cccaaaagcc cggccaatga cactatattc
    12721 tgcctctgga tccacgatga taatttcggt attttcacca gattccttga ttttggtcgt
    12781 gataatctca tgcttggttg ccatcccttt cccagctcca gatgttccta aaatcaaacc
    12841 agacggtgta tttaataggc tgcgatcaat ggtaataata ttgcttgata tttgattgat
    12901 accataatat ttcccactgc ggtcttgtaa gtctactgaa gtccatggag agttcactgc
    12961 tatattggac gttaataaac tccgtgatac gccctctaaa aaatcacaac caaatggcag
    13021 caaactatta aaagctgctt cttgcatata tggaagttta tcaatcatta ggtcatttga
    13081 gccggccact tgttggatag tgtctagggc ttgtttgagt tcttcttcat cctgaccaaa
    13141 aacaccgatc aagaagactg tttgaaatag tttatctcct gtctctgtca tggtttttaa
    13201 gagttcctca gcttcatcga tattactttc taatacatga cctacttttt ccaaatagat
    13261 acctgtacga gctagttttt gttgttcccc aatcttttgg gattccatca aggttttctt
    13321 tgttcgtagt ttcttcatgg catccgcctt agtcgaactt tgggcatgga ggctcacaat
    13381 caattccaaa tctccttgca tgaggtctcg gataaactga tcccctaatt ccataccgta
    13441 gtctctcaca tagacaatct gcaataagcg gtcattgatt tgtaggtaat tcttgttttt
    13501 aaagtccaag agattaggtg ctatgaagtg acgagttgtc tgaccagatt tcgttaaatc
    13561 acggtaagaa aaaggaagat ggtgttctcc tctaagcata tcggccaaca agttcacccg
    13621 ttcttctcca gccaaagatt caaatcgtgc atcaatttct gagaaaccac tcttgaaata
    13681 ttcgcctatt tgagacaagg aacgataggc ttgtttggga ttagaatcct ttctaccaaa
    13741 gctaatcagt ttcacagccg aaaagttatt ttctccactg tctaaatttt gattcatcat
    13801 ccgattcaat tctttacgat agctatcata cccatcttct ttttcctcat acaaaacact
    13861 ttgtctgaat ttttctaaat tcaatctttt attaaagata gtcaattgga agttggtttt
    13921 gtcgtctaaa gagttaatca aatcagaata cttctcaatg attgccccct tatcttctaa
    13981 accaacggtc tggtaattga catcaccaag taaatagctt tgtgaaaaat aatcttcttt
    14041 tacctgcatc agaccgtttt gatacaaggc ttgataagaa agagtattag ccgttgaggg
    14101 taacacttcc tcttttttaa ctttaacttc ttccttttta ttagtcattg aagttttttg
    14161 tttctttaat gtatttgatt ttcttttcat gttcaggtcc tttctttcct gtaattgtgc
    14221 gtaggggaac cgttaattca aaatgaagac ggtatttcaa ataatgttca aaatataaat
    14281 cattgggttt atagactcca aatagcatga ggggaatagt aaaggcaaac acaaaaccgt
    14341 aaacaaacca atctccaaat tgccagaaaa agagattcaa gcccaaaaca ataattgtga
    14401 caataaaggc tggtaaaaca aagatgattt gtcttgtggt gaagcctagc caagccctgt
    14461 gttggtattt tgagatgtct ttaaagacac gtgtattcat gactttcctt tctaaaaagg
    14521 ctaagaagca atcacttcct agcctttatc taattacata cctaagattg agcgagccgt
    14581 acgttgagaa ccaacgaggg caataatcag taagatagct tgtaccaaac taccaaacat
    14641 aatcgcaagt gattgcaaga ctcctgcacc atttgaaaca gctattttcc cagcagattc
    14701 aaataaagga acaagagaaa caatcagaaa aatgagaacc ccttgtaccg catagaccat
    14761 aatatttttt aaatagccaa taccaatgga cttccattca tcgcttaaaa atgttggaat
    14821 cgtaagaggg gcaaatggaa tcatgaggta gagttgaata aatcgaatgg ataccaaaag
    14881 attgaccatg gctgcactta ctatccgaac aagccaaatg aggagggcga aaaagcccac
    14941 aatcatacgg ccaataaatc ctgaaccttt taatccagag attgtatcat actttgcccc
    15001 accgtgagcc acaatcgaag caacttgttc aatagcgtga ctcgcaatcc cgatgatagc
    15061 ttctacaata acggtagtgt tggtaattac aactgcgacc ataatataac taatcaacat
    15121 cggagctaat gcctcaaagg tcatcgctcc accagagtta gcaattttct ttgccatctt
    15181 cgaaaattct aagatgagaa caactgataa aatcgcaact ccaagaggtt gcatgacact
    15241 tttagtaata ctagacatat aagtccaaac ggttggattg tagctagata gagatttaat
    15301 cagatctacc gtagattgta aatctacatt aaatccttca aataaatttt cagctgatat
    15361 tttttcagat gcaaggtaaa caaagggtga gactaaacta agattcatgt cattgtttat
    15421 cctcctaaat tgaaatttga gttacaaagg ctccagcagc cccgaccata actccaccga
    15481 caatttccag aatagcattc cgaacacctg gtccaccatc tttaatgttg gttgcaagat
    15541 tgacaatccc cacaacaacg agaaaggcac caacggcaat caatcccttc tgtaacaaag
    15601 acatagcttg tgcaaacatg gcactagcgt ctactccata gacaaaacct ttaaaatgcg
    15661 taatcatcta tttcctcttt tctattttta ttttaaacta gattcaaaag ttaaatcacg
    15721 aattctaagg ccttcaagat gattttcttg tctttgattt aaaggattga tttgatagtt
    15781 ccaccaccgt tcatcggttt cttgattggc taggtacttc cagtttggat gcttagtaga
    15841 attgtatttt ttgcttttaa agacaggcat attggcaatt cgaaccaggc attcatgccg
    15901 tttcatattt ccgacttcat caggtgtcat tagatcacga gcaatctttt gatgagaaag
    15961 ggatcctgaa cctgtctggc caaaggaacg actagtattt cgaacatcaa tggtttgttt
    16021 accgagtaaa ccactcataa atttaaaggt atcttcatca ttcccaccta agtagactaa
    16081 gctatcacag ttcccaagaa tggttttcca agcttctttt tctttataga gcccttgaag
    16141 ttgggcaata ttttgtagaa taggaacgag actcatgttc cgagaacgga ctgttgaggt
    16201 ttgttcagca aaatctggaa tttctccgat atttgggaac tcatctaagt aaaatctcac
    16261 atgaagaggc aattgcccct taaaatcaat atctgcttgt cttgtcaggg tttgaaatac
    16321 ggttgaaaaa aagagggctg aaagaaagcg aaaggtacta tcgttatctg ggataactaa
    16381 gtaaaccatt gatttttcct tgccccatgt cttcatatca agggtatctc ttttggtcaa
    16441 atccatgaca ctttgaatat tgaagagggc aaatttagca gtggttacag ctataacaga
    16501 atccagggtc ttatccttat aattttgaaa atctgcccaa tttcgcatgg taaaattttc
    16561 agtcccatac tttttagcat aattttcaaa taaaatttct aagacacttt tttcttggtt
    16621 ttcacccttg gataagtgtt taatgagttt tgagatttca gcaaaacttg gataacgccc
    16681 tcgttttttt cgctcttcca cttctttttt ttgacgtttc aacaagtttt ggtattcttt
    16741 ttgacttaaa cgactttctt ctatgagctg ttctcttgtt ttgggtggat tatagaaatc
    16801 gaccaagtag gaggctaaag ctcgtaccaa agtcatagaa gcttcatccc aaaatggatc
    16861 actacgggat ccagagcctt tggtattatt aaaataaacc gtcagcatgc gattcaaatc
    16921 attttctgtc tctatatagc gaaaaggatt gaaaccatct gagttcttca tattgactaa
    16981 atctaacacc tttacttggt aaccattttc taaaaagagt ttacctgttt tctcggccaa
    17041 atgatcttta ggatccacta caatattaga actattcatc tgaatcagat tgggtttcac
    17101 aaagcgaaat gtcttcccac ttcctgaacc tccgatcacc acaatattct tatttctatc
    17161 atattggggt ggttttttat ctaacaatgt caaacgaaca tcttgtgcta agatcgtatc
    17221 atgagaaaat tccttaccgt aaaagagctt cttttctttt agagttccaa aacgggcgct
    17281 cccgtattct accccttctc ggtattgttt tttaccagtc tctagataga gataaaccag
    17341 taacatcatc acaaaaccta gtagaaaaaa aacacttgat tttccagtaa aagaaacatt
    17401 ccatggcgac tgaagaactt gatcttgacc ttccatcaga agatgagtcc atttatctaa
    17461 agtatttcca gtataggaat catacaaaag cgtcaaacga tgaaaaagat agcctagtaa
    17521 gatacctaac agtgagaata gtaggaattt ctttccactg tacatcatct caccatctct
    17581 ttctgtttga cggccccttc ttgtctaaag gttatttggg atttagcttc atcaattgca
    17641 tcgtctaaag acttatccat ggtaaaatca gctaatttct ctggattatt aaccattttt
    17701 tctaacagat ggtctaaatg attgtctaga attgaacggt ctttcgtgta gaaatgcaga
    17761 gaatcccctt gccaagcgat ggctaaagga atctcttctt tttctaaaaa agctttaaat
    17821 ttctctatat caattggttt gtctaaaaaa tcttttttca gattaatcgt atcaatcgaa
    17881 taaggagatt gtagcaattc ttctaatttc tgcaccccta tcttataggc cgaatcctgt
    17941 gctaaagcct gacgtctaga ccattctaga atctttaaaa gactttttac agtaaataaa
    18001 agactacgct ctgcatattg aactgccatt cgttcctgtt gttcagagga catctgatgc
    18061 ctccttctta acaaatagca gctttccttc tttataacga taagctatca atttctgacg
    18121 ttgtttaatc gacttgacaa cctgcagtag atctctcgaa taaggttctc
//
LOCUS       DAVBDG010000169         4730 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 744628.1
            SAMEA1919797-rid20182873.guided.098, whole genome shotgun sequence.
ACCESSION   DAVBDG010000169 DAVBDG010000000
VERSION     DAVBDG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919797
            Sequence Read Archive: ERR298080
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980245.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980245.1
            Genome Coverage       :: 161x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,156
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 61 of 103
            Pseudo Genes (incomplete)         :: 39 of 103
            Pseudo Genes (internal stop)      :: 31 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919797-rid20182873.guided.098"
                     /strain="744628.1"
                     /db_xref="taxon:1313"
     gene            <1..99
                     /locus_tag="VQ526_002153"
     CDS             <1..99
                     /locus_tag="VQ526_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PezT"
                     /protein_id="HET2555791.1"
                     /translation="ADVLQELLFGEWSQVEKDMLKSGEERLKDLTN"
     gene            575..1363
                     /locus_tag="VQ526_002154"
     CDS             575..1363
                     /locus_tag="VQ526_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555792.1"
                     /translation="MTTTKNPWNQLSNVDINGEQAILATEDVELIKKYTSTKHYKNLK
                     DDIYRLQLGFCPQQFVGDIQNADIIVLSKNPGYTPEFKTLYDHDKNYQKTLLNNLQLK
                     GNLYFHAFDLDTNEFGYWAKKFKVWFDDVDNLQDLKEKLPWFSKHVALAEYFPYYSTK
                     YDDKLNDFISKEGYFPTQKFLFNLIRERVLDDNDPVIIIITRSYNKWYDAIPQLKEYK
                     NCYETSNPSNPSLKPENLLKVKRYSAKKEVEKVLEDSLKKLEHK"
     gene            1602..1850
                     /locus_tag="VQ526_002155"
     CDS             1602..1850
                     /locus_tag="VQ526_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2555793.1"
                     /translation="MNYQKLNDITGATKNEKDKYYVYGLYEEGKQLPFYIGKGEGTRL
                     ISHIDEALTEINQEENIQISKKIQIIRKHKGKIIPVII"
     gene            2021..2740
                     /gene="catA"
                     /locus_tag="VQ526_002156"
                     /pseudo
     CDS             2021..2740
                     /gene="catA"
                     /locus_tag="VQ526_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
     gene            complement(3536..4459)
                     /locus_tag="VQ526_002157"
     CDS             complement(3536..4459)
                     /locus_tag="VQ526_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HET2555794.1"
                     /translation="MEKYTEKKQRNQVFQKFIKRHIGENQMDLVEDCNTFLSFVADKT
                     LEKQKLYKANSCKNRFCPVCAWRKARKDALGLSLMMQYIKQQEKKEFIFLTLTTPNVM
                     SDELENEIKRYNNSFRKLIKRKKVGSVIKGYVRKLEITYNKKRDDYNPHFHVLIAVNK
                     SYFTDKRYYISQQEWLDLWRDVTGISEITQVQVQKIRQNNNKELYEMAKYSGKDSDYL
                     INQKVFDAFYKSLKGKQVLVYSGLFKEAKKKLKNGDLDYLKEIDPTEYIYQIFYIWKQ
                     KEYLASELYDLTEQEKREINHKMIDEIEEEQ"
BASE COUNT         1677 a          720 c          649 g         1684 t
ORIGIN      
        1 gcagatgttc ttcaagagtt actgtttggg gagtggagtc aagtagagaa ggatatgctt
       61 aaatctggag aagaaagatt gaaagattta actaattgaa atggttgtta gatcttgagt
      121 tttaaaataa agcctattaa aaattgctat ctcttagatt atggtaaaaa aatggctttt
      181 aattaaacta acaaagtgag tataaataaa agtaataaat ctcaccgtca ttctttttta
      241 aattaatcgt tgttctcaaa atatttccaa tttatcggac tttttgtact agtcttatac
      301 tttttagtta attctatctg gcctatagta ttgaaaataa tttgtagact tggcaatgag
      361 aattcgttac agtttagtag agccgacgta ttccgaacat catttacggc ggtgtgaaag
      421 gtgatataaa taatattata tctattgata ttcaaaatcg tttcctcact cagattcttt
      481 tataatcgag aatatattaa taaaatctat ttttgtattt aatattaaaa catcgtaaaa
      541 tatagtaatt ctacaaaaag aaaggaaaaa taaaatgact acaactaaaa atccctggaa
      601 tcaattatct aacgtcgata taaatggaga gcaagctatt cttgctaccg aagatgttga
      661 actaatcaaa aaatacacct ctacaaaaca ctacaaaaat ctaaaagatg atatctaccg
      721 tttacaatta ggtttttgtc cgcaacaatt tgtgggggat attcaaaatg ctgatatcat
      781 tgttttatct aaaaatcctg gctacacccc tgaatttaaa acactttatg accacgataa
      841 aaactaccaa aagactcttt taaataatct acagctaaaa ggaaacctct attttcatgc
      901 ttttgaccta gatacgaatg aatttggtta ctgggctaaa aagtttaaag tatggtttga
      961 tgatgtagac aatttacagg atttaaaaga aaagcttcct tggttttcaa aacacgttgc
     1021 tttagctgaa tattttccat attactctac taagtacgat gataaattaa atgattttat
     1081 aagcaaagaa ggttatttcc caacacaaaa gtttttattc aatttaatta gagaacgtgt
     1141 tttggatgac aatgatcctg taatcattat catcacacga tcttacaata agtggtacga
     1201 tgcaattcct caattaaaag agtataaaaa ttgttatgaa acgagtaatc ctagtaatcc
     1261 ttctttaaaa cctgagaatt tattaaaagt caaacgatat tctgctaaaa aagaggttga
     1321 aaaagtatta gaagattcat tgaaaaagct agagcataaa taaaacagtc tctttttaat
     1381 gctatcgtaa atactgtatt gtgtagttat tagtaaacct atatattgtc cccaaattta
     1441 tttagattat tctctaggat tttacttgat tttaaagaaa atatagttta acatataaat
     1501 agttcagaat cagaatgaag gtagaataag aggattattt taattaattt ttaatagtat
     1561 ggagcagaac ttttataata tatttaaata ggagtaaaaa aatgaattat caaaaattaa
     1621 atgatattac tggtgctacc aaaaatgaaa aagataaata ctatgtttac ggactctatg
     1681 aagaaggtaa gcaactacct ttctatattg gtaaaggaga gggaactcgc ctaatatcac
     1741 atatcgatga agcgcttaca gagataaatc aagaagaaaa catacaaatt agtaaaaaaa
     1801 ttcaaattat tagaaaacat aagggcaaaa ttatacctgt aatcatttaa agcacccatt
     1861 agttcaacaa acgaaaattg gataaagtgg gatattttta aaatatatat atttatgtta
     1921 cagtaatatt gacttttaaa aaaggattga ttctaatgaa gaaagcagac aagtaagcct
     1981 cctaaattca ctttagataa aaatttagga ggcatatcaa atgaacttta ataaaattga
     2041 tttagacaat tggaagagaa aagagatatt taatcattat ttgaaccaac aaacgacttt
     2101 tagtataacc acagaaattg atattagtgt tttataccga aacataaaac aagaaggata
     2161 taaattttac cctgcattta ttttcttagt gacaagggtg ataaactcaa atacagcttt
     2221 tagaactggt tacaatagcg acggagagtt aggttattgg gataagttag agccacttta
     2281 tacaattttt gatggtgtat ctaaaacatt ctctggtatt tggactcctg taaagaatga
     2341 cttcaaagag ttttatgatt tatacctttc tgatgtagag aaatataatg gttcggggaa
     2401 attgtttccc aaaacaccta tacctgaaaa tgctttttct ctttctatta ttccatggac
     2461 ttcatttact gggtttaact taaatatcaa taataatagt aattaccttc tacccattat
     2521 tacagcagga aaattcatta ataaaggtaa ttcaatatat ttaccgctat ctttacaggt
     2581 acatcattct gtttgtgatg gttatcatgc aggattgttt atgaactcta ttcaggaatt
     2641 gtcagatagg cctaatgact ggcttttata atatgagata atgccgactg tactttttac
     2701 agtcggtttt ctaatgtcac taacctgccc cgttagttga agaaggtttt tatattacag
     2761 ctccagatcc atatccttct ttttctgaac cgacttctcc tttttcgctt ctttattcca
     2821 attgctttat tgacgttgag cctcggaacc cttaacaatc ccaaaacttg tcgaatggtc
     2881 ggcttaataa ctcacgctat gccgacattc gtctgcaagt ttagttaagg gttcttctca
     2941 acgcacaata aattttctcg gcataaatgc gtggtctaat ttttattttt aataaccttg
     3001 atagcaaaaa atgccattcc aatacaaaac cacataccta taatcgataa ccacataaca
     3061 gtcataaaac cactcctttt taacaaactt tatcacaaga aatatttaaa ttttaaatgc
     3121 ctttattttg aattttaagg ggcattttaa agatttaggg gtaaatcata tagttttatg
     3181 cctaaaaacc tacagaagct tttaaaaagc aaatatgagc caaataaata tattctaatt
     3241 ctacaaacaa aaatttgagc aaattcagtg tcgatttttt aagacactgc ccagttacat
     3301 gcaaattaaa attttcatga ttttttatag ttcctaacag ggttaaaatt tgtataacga
     3361 aagtataatg tttatataac gttagtataa taaagcattt taacattata cttttgataa
     3421 tcgtttatcg tcgtcatcac aataactttt aaaatactcg tgcataattc aacagctgac
     3481 ctcccaataa ctacatggtg ttatcgggag gtcagctgtt agcacttata ttttgttatt
     3541 gttcttcctc gatttcgtct atcattttgt gattaatttc tcttttttct tgttctgtta
     3601 agtcataaag ttcactagct aaatactctt tttgtttcca aatataaaaa atttgataga
     3661 tatattcggt tggatcaatt tcttttaagt aatctaaatc cccatttttt aatttctttt
     3721 tagcctcttt aaataatcct gaataaacta atacctgttt acctttaagt gatttataaa
     3781 atgcatcaaa gactttttga tttattaaat aatcactatc tttaccagaa tacttagcca
     3841 tttcatataa ttctttatta ttattttgtc ttattttttg aacttgaact tgtgttattt
     3901 ctgaaatgcc cgttacatca cgccataaat ctaaccattc ttgttggcta atataatatc
     3961 ttttatctgt gaaatacgat ttatttactg caattaacac atgaaaatga ggattataat
     4021 catctctttt tttattatat gtaatctcta acttacgaac atatcccttt ataacactac
     4081 ctactttttt tctctttata agttttctaa aagaattatt ataacgtttt atttcatttt
     4141 ctaattcatc actcattaca ttaggtgtag tcaaagttaa aaagataaac tcctttttct
     4201 cttgctgctt aatatattgc atcatcaaag ataaacccaa tgcatctttt ctagcttttc
     4261 tccaagcaca gacaggacaa aatcgatttt tacaagaatt agctttatat aatttctgtt
     4321 tttctaaagt tttatcagct acaaaagaca gaaatgtatt gcaatcttca actaaatcca
     4381 tttgattctc tccaatatga cgtttaataa atttctgaaa tacttgattt ctttgttttt
     4441 tctcagtata cttttccatg ttataacaca taaaaacaac ttagttttca caaactatga
     4501 caataaaaaa agttgctttt tcccctttct atgtatgttt tttactagtc atttaaaacg
     4561 atacattaat aggtacgaaa aagcaacttt ttttgcgctt aaaaccagtc ataccaataa
     4621 cttaagggta actagcctcg ccggcaatag ttacccctat tattaaatac agctaatcca
     4681 gaggtcaagg attatctttt aaaggttgcg acttattgga ttgaagagtt
//
